PIGA protein

Overview
NamePIGA protein
Smed IDSMED30004543
Length (bp)656
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004543

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30004543SMESG000067725.1 SmedASXL_015540SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30004543SMESG000067725.1 dd_Smed_v4_5228_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of PIGA protein vs. TrEMBL
Match: A0A4E0QVR4 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_009859 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 3.209e-18
Identity = 47/124 (37.90%), Postives = 75/124 (60.48%), Query Frame = 2 
Query:  110 MSESPPINPLGSSLSIGRYHRSDSNASRLSTVSQKSCNISSEANRKSAP-TTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAEL 478
            M+E    N    SL         S+ + + ++ + S  + +       P +T   RRVSS+ L++L + E+ D+HPYGRGGIYEG LYS +K   +  V+I++WL+F S++++ S+A+VFVAEL
Sbjct:   45 MAEETAPNKTAGSLG--------SDQTMIKSILKSSLGLDTMTGISDIPLSTVDQRRVSSITLEMLMEREEIDVHPYGRGGIYEGILYSHKKEEELDFVVILVWLIFSSSVLMISIAVVFVAEL 160
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BLAST of PIGA protein vs. TrEMBL
Match: A0A2H1BUQ6 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_14327 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 4.152e-18
Identity = 47/124 (37.90%), Postives = 75/124 (60.48%), Query Frame = 2 
Query:  110 MSESPPINPLGSSLSIGRYHRSDSNASRLSTVSQKSCNISSEANRKSAP-TTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAEL 478
            M+E    N    SL         S+ + + ++ + S  + +       P +T   RRVSS+ L++L + E+ D+HPYGRGGIYEG LYS +K   +  V+I++WL+F S++++ S+A+VFVAEL
Sbjct:   48 MAEETAPNKTAGSLG--------SDQTMIKSILKSSLGLDTMTGISDIPLSTVDQRRVSSITLEMLMEREEIDVHPYGRGGIYEGILYSHKKEEELDFVVILVWLIFSSSVLMISIAVVFVAEL 163
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BLAST of PIGA protein vs. TrEMBL
Match: A0A4S2LFB0 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008011 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 7.292e-18
Identity = 40/101 (39.60%), Postives = 69/101 (68.32%), Query Frame = 2 
Query:  182 NASRLSTVSQKSCNISSE--ANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAEL 478
            N   L ++ + S  + S+  A   +A +T   RR+SS+ L++L + E+ DIHPYGRGGIYEG LY++++   V  + +++W +FF +L++ +++I+FVAEL
Sbjct:   57 NPEALKSILKMSLGLGSQVSAPESTAISTETQRRLSSVTLEMLMEREEIDIHPYGRGGIYEGILYTRKEGEPVDMLGLVVWFIFFGSLVMMAVSIIFVAEL 157
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BLAST of PIGA protein vs. TrEMBL
Match: A0A1S8WY07 (PIGA protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_04731 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.337e-14
Identity = 37/99 (37.37%), Postives = 66/99 (66.67%), Query Frame = 2 
Query:  182 NASRLSTVSQKSCNISSE--ANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVA 472
            N   L ++ + S  +  +  A   +A +T   RR+SS+ L++L + E+ DIHPYGRGGIYEG LY++++   V  + +++W +FF +L++ +++I+FVA
Sbjct:  438 NPEALKSILKMSLGLGPQVLAPESTAISTETQRRLSSVTLEMLMEREEIDIHPYGRGGIYEGILYTRKEEEPVDMLGLVVWFIFFGSLVMMAVSIIFVA 536
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BLAST of PIGA protein vs. TrEMBL
Match: A0A430PY64 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0009122 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 3.373e-13
Identity = 39/110 (35.45%), Postives = 63/110 (57.27%), Query Frame = 2 
Query:  155 IGRYHRSDS---NASRLSTVSQKSCNISSEANRKSAPTTPKHR-RVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVA 472
            +G  H S S   N  +L T  +++  +     ++      +H+ R+S LA+++L + ED D+HPYGRGGIYEG LY  R    ++ ++  +W VF S++ I +  IV VA
Sbjct:   33 VGCCHHSHSDSENLEKLKTALRQALGLEPNLPKQCVMMDLEHQNRLSLLAIEMLIEQEDIDVHPYGRGGIYEGILYRNRPKEKINIILAFIWFVFVSSVAIMAGTIVTVA 142
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BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000081746.1 (SMESG000081746.1)

HSP 1 Score: 65.4698 bits (158), Expect = 1.169e-13
Identity = 30/80 (37.50%), Postives = 48/80 (60.00%), Query Frame = 2 
Query:  248 SAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRK-STFVSPVMIILWLVFFSALIIGSLAIVFVAELYR 484
            + P   K  R SS+ALD+L  ++++DIHP+GRGG + G LY  +K    +    I +W++F    + GS  I+ +AE+ R
Sbjct:   33 TNPNKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDR 112
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BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000079358.1 (SMESG000079358.1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.509e-13
Identity = 41/101 (40.59%), Postives = 57/101 (56.44%), Query Frame = 2 
Query:  224 ISSEANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTF-VSPVMIILWLVFFSALIIGSLAIVFVAELYRHSKNATAIDPNQS 523
            ISS    K     PK  R S++A+DIL  +ED DIHPYGRGG +EG LY+ RK    +     + WL     L++ SL I+F+AE +R   N   +  N++
Sbjct:   46 ISSYNKDKLYSNVPKVPRFSTVAMDILRSTEDHDIHPYGRGGRFEGVLYTGRKIRIRLESNKWLAWLTLIGLLLMLSLTIIFLAEQHRDMVNGVNLFDNET 146
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BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000048041.1 (SMESG000048041.1)

HSP 1 Score: 56.225 bits (134), Expect = 3.779e-10
Identity = 28/85 (32.94%), Postives = 41/85 (48.24%), Query Frame = 2 
Query:  230 SEANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAELYR 484
            +E    S  T    RR S +A+ +L   ++ D HPYGRG   +G              MII+W +F   L+   + I+ +AELYR
Sbjct:   39 TEKGGGSNSTILNIRRFSDIAITMLKDKDETDEHPYGRGKTVDGCFNRSSSGKDSDQSMIIVWFIFVGFLLGSCVMIIILAELYR 123
The following BLAST results are available for this feature:
BLAST of PIGA protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0QVR43.209e-1837.90Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A2H1BUQ64.152e-1837.90Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A4S2LFB07.292e-1839.60Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A1S8WY072.337e-1437.37PIGA protein (Fragment) OS=Opisthorchis viverrini ... [more]
A0A430PY643.373e-1335.45Uncharacterized protein OS=Schistosoma bovis OX=61... [more]
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BLAST of PIGA protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PIGA protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000081746.11.169e-1337.50SMESG000081746.1[more]
SMESG000079358.15.509e-1340.59SMESG000079358.1[more]
SMESG000048041.13.779e-1032.94SMESG000048041.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004543 ID=SMED30004543|Name=PIGA protein|organism=Schmidtea mediterranea sexual|type=transcript|length=656bp
ATTTCATAGAACTTTTATTTTTGGAAGTTTACATTTATTTGTCAGTTTTG
TACTAAAACGAGACATGGAACAATTTCAAGATGATGTGAAAAATAATTCA
ATGTTACCAATGTCTGAAAGTCCTCCAATAAACCCTCTTGGTAGCTCATT
GTCAATTGGTAGATATCATAGATCTGATAGTAATGCTTCTCGGCTTTCCA
CGGTTTCTCAAAAAAGTTGCAATATTTCTTCAGAAGCTAATAGAAAATCT
GCTCCTACAACTCCAAAACATAGAAGGGTTTCATCATTAGCATTGGACAT
TCTCACTCAATCAGAAGATAAAGACATCCATCCGTATGGTCGAGGAGGTA
TTTATGAAGGATCATTGTATAGTAAAAGAAAAAGCACTTTTGTCTCTCCA
GTTATGATAATTTTGTGGCTTGTATTCTTTAGTGCTTTAATTATTGGATC
ACTGGCAATTGTTTTTGTTGCAGAGCTTTACCGGCACTCCAAGAATGCCA
CCGCAATCGATCCCAATCAATCTCCATGGAAACCGAAGTGTCACTGACAA
CCGAAAATGCATTAAAATAAGACAACATTACATTAGTTGATAATAAAACA
TTGAAATTGATAAATAAATAAATGAAAATATAAACAACATTTAATGCGAA
AAAAAA
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protein sequence of SMED30004543-orf-1

>SMED30004543-orf-1 ID=SMED30004543-orf-1|Name=SMED30004543-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=182bp
FHRTFIFGSLHLFVSFVLKRDMEQFQDDVKNNSMLPMSESPPINPLGSSL
SIGRYHRSDSNASRLSTVSQKSCNISSEANRKSAPTTPKHRRVSSLALDI
LTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGS
LAIVFVAELYRHSKNATAIDPNQSPWKPKCH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006506GPI anchor biosynthetic process
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..90
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..83
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.548
NoneNo IPR availableTMHMMTMhelixcoord: 135..157