PIGA protein
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004543 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of PIGA protein vs. TrEMBL
Match: A0A4E0QVR4 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_009859 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 3.209e-18 Identity = 47/124 (37.90%), Postives = 75/124 (60.48%), Query Frame = 2 Query: 110 MSESPPINPLGSSLSIGRYHRSDSNASRLSTVSQKSCNISSEANRKSAP-TTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAEL 478 M+E N SL S+ + + ++ + S + + P +T RRVSS+ L++L + E+ D+HPYGRGGIYEG LYS +K + V+I++WL+F S++++ S+A+VFVAEL Sbjct: 45 MAEETAPNKTAGSLG--------SDQTMIKSILKSSLGLDTMTGISDIPLSTVDQRRVSSITLEMLMEREEIDVHPYGRGGIYEGILYSHKKEEELDFVVILVWLIFSSSVLMISIAVVFVAEL 160
BLAST of PIGA protein vs. TrEMBL
Match: A0A2H1BUQ6 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_14327 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 4.152e-18 Identity = 47/124 (37.90%), Postives = 75/124 (60.48%), Query Frame = 2 Query: 110 MSESPPINPLGSSLSIGRYHRSDSNASRLSTVSQKSCNISSEANRKSAP-TTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAEL 478 M+E N SL S+ + + ++ + S + + P +T RRVSS+ L++L + E+ D+HPYGRGGIYEG LYS +K + V+I++WL+F S++++ S+A+VFVAEL Sbjct: 48 MAEETAPNKTAGSLG--------SDQTMIKSILKSSLGLDTMTGISDIPLSTVDQRRVSSITLEMLMEREEIDVHPYGRGGIYEGILYSHKKEEELDFVVILVWLIFSSSVLMISIAVVFVAEL 163
BLAST of PIGA protein vs. TrEMBL
Match: A0A4S2LFB0 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008011 PE=4 SV=1) HSP 1 Score: 87.8113 bits (216), Expect = 7.292e-18 Identity = 40/101 (39.60%), Postives = 69/101 (68.32%), Query Frame = 2 Query: 182 NASRLSTVSQKSCNISSE--ANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAEL 478 N L ++ + S + S+ A +A +T RR+SS+ L++L + E+ DIHPYGRGGIYEG LY++++ V + +++W +FF +L++ +++I+FVAEL Sbjct: 57 NPEALKSILKMSLGLGSQVSAPESTAISTETQRRLSSVTLEMLMEREEIDIHPYGRGGIYEGILYTRKEGEPVDMLGLVVWFIFFGSLVMMAVSIIFVAEL 157
BLAST of PIGA protein vs. TrEMBL
Match: A0A1S8WY07 (PIGA protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_04731 PE=4 SV=1) HSP 1 Score: 82.0333 bits (201), Expect = 2.337e-14 Identity = 37/99 (37.37%), Postives = 66/99 (66.67%), Query Frame = 2 Query: 182 NASRLSTVSQKSCNISSE--ANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVA 472 N L ++ + S + + A +A +T RR+SS+ L++L + E+ DIHPYGRGGIYEG LY++++ V + +++W +FF +L++ +++I+FVA Sbjct: 438 NPEALKSILKMSLGLGPQVLAPESTAISTETQRRLSSVTLEMLMEREEIDIHPYGRGGIYEGILYTRKEEEPVDMLGLVVWFIFFGSLVMMAVSIIFVA 536
BLAST of PIGA protein vs. TrEMBL
Match: A0A430PY64 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0009122 PE=4 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 3.373e-13 Identity = 39/110 (35.45%), Postives = 63/110 (57.27%), Query Frame = 2 Query: 155 IGRYHRSDS---NASRLSTVSQKSCNISSEANRKSAPTTPKHR-RVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVA 472 +G H S S N +L T +++ + ++ +H+ R+S LA+++L + ED D+HPYGRGGIYEG LY R ++ ++ +W VF S++ I + IV VA Sbjct: 33 VGCCHHSHSDSENLEKLKTALRQALGLEPNLPKQCVMMDLEHQNRLSLLAIEMLIEQEDIDVHPYGRGGIYEGILYRNRPKEKINIILAFIWFVFVSSVAIMAGTIVTVA 142
BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000081746.1 (SMESG000081746.1) HSP 1 Score: 65.4698 bits (158), Expect = 1.169e-13 Identity = 30/80 (37.50%), Postives = 48/80 (60.00%), Query Frame = 2 Query: 248 SAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRK-STFVSPVMIILWLVFFSALIIGSLAIVFVAELYR 484 + P K R SS+ALD+L ++++DIHP+GRGG + G LY +K + I +W++F + GS I+ +AE+ R Sbjct: 33 TNPNKSKQHRFSSVALDLLRVNDNEDIHPFGRGGKFTGVLYGNQKIKNRIDHSKIFVWILFIGFALTGSALIIILAEIDR 112
BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000079358.1 (SMESG000079358.1) HSP 1 Score: 64.3142 bits (155), Expect = 5.509e-13 Identity = 41/101 (40.59%), Postives = 57/101 (56.44%), Query Frame = 2 Query: 224 ISSEANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTF-VSPVMIILWLVFFSALIIGSLAIVFVAELYRHSKNATAIDPNQS 523 ISS K PK R S++A+DIL +ED DIHPYGRGG +EG LY+ RK + + WL L++ SL I+F+AE +R N + N++ Sbjct: 46 ISSYNKDKLYSNVPKVPRFSTVAMDILRSTEDHDIHPYGRGGRFEGVLYTGRKIRIRLESNKWLAWLTLIGLLLMLSLTIIFLAEQHRDMVNGVNLFDNET 146
BLAST of PIGA protein vs. Planmine SMEST
Match: SMESG000048041.1 (SMESG000048041.1) HSP 1 Score: 56.225 bits (134), Expect = 3.779e-10 Identity = 28/85 (32.94%), Postives = 41/85 (48.24%), Query Frame = 2 Query: 230 SEANRKSAPTTPKHRRVSSLALDILTQSEDKDIHPYGRGGIYEGSLYSKRKSTFVSPVMIILWLVFFSALIIGSLAIVFVAELYR 484 +E S T RR S +A+ +L ++ D HPYGRG +G MII+W +F L+ + I+ +AELYR Sbjct: 39 TEKGGGSNSTILNIRRFSDIAITMLKDKDETDEHPYGRGKTVDGCFNRSSSGKDSDQSMIIVWFIFVGFLLGSCVMIIILAELYR 123 The following BLAST results are available for this feature:
BLAST of PIGA protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of PIGA protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of PIGA protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of PIGA protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of PIGA protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of PIGA protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of PIGA protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of PIGA protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004543 ID=SMED30004543|Name=PIGA protein|organism=Schmidtea mediterranea sexual|type=transcript|length=656bpback to top protein sequence of SMED30004543-orf-1 >SMED30004543-orf-1 ID=SMED30004543-orf-1|Name=SMED30004543-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=182bp FHRTFIFGSLHLFVSFVLKRDMEQFQDDVKNNSMLPMSESPPINPLGSSLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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