SMED30004541

Overview
NameSMED30004541
Smed IDSMED30004541
Length (bp)2509
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30004541 (SMED30004541) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30004541 (SMED30004541) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30004541 (SMED30004541) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30004541 (SMED30004541) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004541

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30004541h1SMcG0012223 SmedASXL_016797SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30004541h1SMcG0012223 dd_Smed_v4_13044_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004541h1SMcG0012223 dd_Smed_v4_13044_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004541h1SMcG0012223 dd_Smed_v4_13044_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30004541h1SMcG0012223 dd_Smed_v4_13044_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30004541h1SMcG0012223 dd_Smed_v4_13044_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30004541 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001525:2923..6886 -12373
Homology
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BLAST of SMED30004541 vs. Planmine SMEST
Match: SMESG000056767.1 (SMESG000056767.1)

HSP 1 Score: 985.712 bits (2547), Expect = 0.000e+0
Identity = 472/495 (95.35%), Postives = 472/495 (95.35%), Query Frame = 1 
Query:   55 MSFVSKWFQKCHAARLSKYNQEVINKEHFIDKYPKFPFSTNFLNEIMILTYGYENSIADDDSFPVSHIQWNEKIFYGYKEISYLKLLDMKCESSLKSANSKNLEDIHVDRNHSPSIDESTFFNAKEPSGSNLAETVKDVRCWFPLWIDGVCKIVCQLHQPQRTVITGAVLSYSHSQKLFTTLYCSYKFTDGIDIEFMGKYVKFLQEMPLELLGKFDGDNWLQFALKLSLLSRNGFFACSSKPITKLSGCRRSQFINNIIGRAQLKKQLKRPRNSERPVNYLSICEELETIASAIQESARDWVTMFQAFLLRHGENVSEKFAGIDSQNPVARLLIXXXXXXXXXXXXXXSLFHSRPVDLDTSQESISYENFSGGEKCNSTFTNLDGKLVLWSVTRNPIGQFCISGMKLTNGRQRFIEIDPVAIGSVMNSGKAVIADKCCYELLGPMYPLSIRLAVPSASNIDYIWPLLTHFTNGLPLHWKCFAQAIDVLCKCSPLR 1539
            MSFVSKWFQKCHAARLSKYNQEVINKEHFIDKYPKFPFSTNFLNEIMILTYGYENSIADDDSFPVSHIQWNEKIFYGYKEISYLKLLDMKCESSLKSANSKNLEDIHVDRNHSPSIDESTFFNAKEPSGSNLAETVKDVRCWFPLWIDGVCKIVCQLHQPQRTVITGAVLSYSHSQKLFTTLYCSYKFTDGIDIEFMGKYVKFLQEMPLELLGKFDGDNWLQFALKLSLLSRNGFFACSSKPITKLSGCRRSQFINNIIGRAQLKKQLKRPRNSERPVNYLSICEELETIASAIQESARDWVTMFQAFLLRHGENVSEKFAGIDSQNPVA                       RPVDLDTSQESISYENFSGGEKCNSTFTNLDGKLVLWSVTRNPIGQFCISGMKLTNGRQRFIEIDPVAIGSVMNSGKAVIADKCCYELLGPMYPLSIRLAVPSASNIDYIWPLLTHFTNGLPLHWKCFAQAIDVLCKCSPLR
Sbjct:    1 MSFVSKWFQKCHAARLSKYNQEVINKEHFIDKYPKFPFSTNFLNEIMILTYGYENSIADDDSFPVSHIQWNEKIFYGYKEISYLKLLDMKCESSLKSANSKNLEDIHVDRNHSPSIDESTFFNAKEPSGSNLAETVKDVRCWFPLWIDGVCKIVCQLHQPQRTVITGAVLSYSHSQKLFTTLYCSYKFTDGIDIEFMGKYVKFLQEMPLELLGKFDGDNWLQFALKLSLLSRNGFFACSSKPITKLSGCRRSQFINNIIGRAQLKKQLKRPRNSERPVNYLSICEELETIASAIQESARDWVTMFQAFLLRHGENVSEKFAGIDSQNPVA-----------------------RPVDLDTSQESISYENFSGGEKCNSTFTNLDGKLVLWSVTRNPIGQFCISGMKLTNGRQRFIEIDPVAIGSVMNSGKAVIADKCCYELLGPMYPLSIRLAVPSASNIDYIWPLLTHFTNGLPLHWKCFAQAIDVLCKCSPLR 472

HSP 2 Score: 291.582 bits (745), Expect = 2.382e-130
Identity = 156/157 (99.36%), Postives = 156/157 (99.36%), Query Frame = 1 
Query: 2020 GHSVGMSSQLPMKKGQLQNQRVKKKKCSFAIPLIXXXXXXXXXXXXXXGPAIRGVEREPQLNYRKKAVKNSQQHRARSSLFDVDFTPSRRHSLDYSQYIVEYMCLNDMVQTTRSGRKSLPPLDPRFDLEYKIENEKVFLLSNGEKFELASRVARSHR 2490
             HSVGMSSQLPMKKGQLQNQRVKKKKCSFAIPLISMSRSDFSRSRSVSGPAIRGVEREPQLNYRKKAVKNSQQHRARSSLFDVDFTPSRRHSLDYSQYIVEYMCLNDMVQTTRSGRKSLPPLDPRFDLEYKIENEKVFLLSNGEKFELASRVARSHR
Sbjct:  583 SHSVGMSSQLPMKKGQLQNQRVKKKKCSFAIPLISMSRSDFSRSRSVSGPAIRGVEREPQLNYRKKAVKNSQQHRARSSLFDVDFTPSRRHSLDYSQYIVEYMCLNDMVQTTRSGRKSLPPLDPRFDLEYKIENEKVFLLSNGEKFELASRVARSHR 739

HSP 3 Score: 195.282 bits (495), Expect = 2.382e-130
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 2 
Query: 1640 RPHESSSMLFLTPIPAXXXXXXXXXXXXHTISDTNTILMMPDVRLDRLPIVVEKAVDRSSDANIHQSPTTRSILDISPVLPQRPVVRNHEEPPVSSVAVTTINTRKRRTESSKS 1981
            RPHESSSMLFLTPIPASRSRRNSQSSNSHTISDTNTILMMPDVRLDRLPIVVEKAVDRSSDANIHQSPTTRSILDISPVLPQRPVVRNHEEPPVSSVAVTTINTRKRRTESS S
Sbjct:  472 RPHESSSMLFLTPIPASRSRRNSQSSNSHTISDTNTILMMPDVRLDRLPIVVEKAVDRSSDANIHQSPTTRSILDISPVLPQRPVVRNHEEPPVSSVAVTTINTRKRRTESSHS 585
[Close]
BLAST of SMED30004541 vs. Planmine SMEST
Match: SMESG000016241.1 (SMESG000016241.1)

HSP 1 Score: 379.793 bits (974), Expect = 1.230e-116
Identity = 199/376 (52.93%), Postives = 250/376 (66.49%), Query Frame = 1 
Query:  457 TVKDVRCWFPLWIDGVCKIVCQLH----QPQRTVITGAVLSYSHSQKLFTTLYCSYKFTDGIDIEFMGKYVKFLQEMPLELLGKFDGDNWLQFALKLSLLSRNGFFACSSKPITKLSGCRRSQFINNIIGRAQLKKQLKRPRNSERPVNYLSICEELETIASAIQESARDWVTMFQAFLLRHGENV--SEKFAGIDSQNPVARLLIXXXXXXXXXXXXXXSLFHSRPVDLDTSQESISYENFSGGEKCNSTFTNLDGKLVLWSVTRNPIGQFCISGMKLTNGRQRFIEIDPVAIGSVMNSGKAVIADKCCYELLGPMYPLSIRLAVPSASNIDYIWPLLTHFTNGLPLHWKCFAQAIDVLCKCSPLRYSSISLDDC 1566
            TV+ +  WFP+WIDGVCK+V Q+     Q  +T ITGAVLSY HS+KLFTTL CSY FTDGIDIEFMGKYVKFLQE PLELLGKFDGDNWL+FA+KLSLLSRNGFFACSS+   K      +  +  ++ +  +KK        E+P +YLS+C+ELETIA+ I ++  +W+  F+  L ++G+NV  +EK   I   N                         S+PVDLDT   +IS ++F+GGEK +ST  N + KLVLWSVT N  GQ CISG +LT+  + F EI P  I SV N+ +AVIA+KCCYEL+GPMY LS+RLA P A N++Y+WP  T+FT+ LP +WK F  AID LCKCSPLR  S    D 
Sbjct:  478 TVRIIHRWFPIWIDGVCKLVGQIQNSSNQSHKTAITGAVLSYCHSEKLFTTLDCSYIFTDGIDIEFMGKYVKFLQEKPLELLGKFDGDNWLKFAIKLSLLSRNGFFACSSESFKKSLQTNPNSVLLKVLCKTPVKKIYSGTPKVEQPPHYLSVCKELETIANLIGKNG-NWLHGFEKLLQKYGQNVILNEKLKSIMDSN------------------------LSKPVDLDT---TISLDDFNGGEKFSSTLVNFESKLVLWSVTVNTNGQLCISGFQLTDSNKLFNEIKPTPISSVTNNRRAVIANKCCYELVGPMYTLSLRLAYPEAVNMEYLWPFQTYFTSCLPSNWKAFILAIDTLCKCSPLRKESCEAADV 825
[Close]
BLAST of SMED30004541 vs. Planmine SMEST
Match: SMESG000016241.1 (SMESG000016241.1)

HSP 1 Score: 380.178 bits (975), Expect = 2.828e-116
Identity = 199/376 (52.93%), Postives = 250/376 (66.49%), Query Frame = 1 
Query:  457 TVKDVRCWFPLWIDGVCKIVCQLH----QPQRTVITGAVLSYSHSQKLFTTLYCSYKFTDGIDIEFMGKYVKFLQEMPLELLGKFDGDNWLQFALKLSLLSRNGFFACSSKPITKLSGCRRSQFINNIIGRAQLKKQLKRPRNSERPVNYLSICEELETIASAIQESARDWVTMFQAFLLRHGENV--SEKFAGIDSQNPVARLLIXXXXXXXXXXXXXXSLFHSRPVDLDTSQESISYENFSGGEKCNSTFTNLDGKLVLWSVTRNPIGQFCISGMKLTNGRQRFIEIDPVAIGSVMNSGKAVIADKCCYELLGPMYPLSIRLAVPSASNIDYIWPLLTHFTNGLPLHWKCFAQAIDVLCKCSPLRYSSISLDDC 1566
            TV+ +  WFP+WIDGVCK+V Q+     Q  +T ITGAVLSY HS+KLFTTL CSY FTDGIDIEFMGKYVKFLQE PLELLGKFDGDNWL+FA+KLSLLSRNGFFACSS+   K      +  +  ++ +  +KK        E+P +YLS+C+ELETIA+ I ++  +W+  F+  L ++G+NV  +EK   I   N                         S+PVDLDT   +IS ++F+GGEK +ST  N + KLVLWSVT N  GQ CISG +LT+  + F EI P  I SV N+ +AVIA+KCCYEL+GPMY LS+RLA P A N++Y+WP  T+FT+ LP +WK F  AID LCKCSPLR  S    D 
Sbjct:  531 TVRIIHRWFPIWIDGVCKLVGQIQNSSNQSHKTAITGAVLSYCHSEKLFTTLDCSYIFTDGIDIEFMGKYVKFLQEKPLELLGKFDGDNWLKFAIKLSLLSRNGFFACSSESFKKSLQTNPNSVLLKVLCKTPVKKIYSGTPKVEQPPHYLSVCKELETIANLIGKNG-NWLHGFEKLLQKYGQNVILNEKLKSIMDSN------------------------LSKPVDLDT---TISLDDFNGGEKFSSTLVNFESKLVLWSVTVNTNGQLCISGFQLTDSNKLFNEIKPTPISSVTNNRRAVIANKCCYELVGPMYTLSLRLAYPEAVNMEYLWPFQTYFTSCLPSNWKAFILAIDTLCKCSPLRKESCEAADV 878
[Close]
BLAST of SMED30004541 vs. Planmine SMEST
Match: SMESG000016241.1 (SMESG000016241.1)

HSP 1 Score: 337.035 bits (863), Expect = 1.559e-100
Identity = 178/338 (52.66%), Postives = 224/338 (66.27%), Query Frame = 1 
Query:  559 VLSYSHSQKLFTTLYCSYKFTDGIDIEFMGKYVKFLQEMPLELLGKFDGDNWLQFALKLSLLSRNGFFACSSKPITKLSGCRRSQFINNIIGRAQLKKQLKRPRNSERPVNYLSICEELETIASAIQESARDWVTMFQAFLLRHGENV--SEKFAGIDSQNPVARLLIXXXXXXXXXXXXXXSLFHSRPVDLDTSQESISYENFSGGEKCNSTFTNLDGKLVLWSVTRNPIGQFCISGMKLTNGRQRFIEIDPVAIGSVMNSGKAVIADKCCYELLGPMYPLSIRLAVPSASNIDYIWPLLTHFTNGLPLHWKCFAQAIDVLCKCSPLRYSSISLDDC 1566
            +LSY HS+KLFTTL CSY FTDGIDIEFMGKYVKFLQE PLELLGKFDGDNWL+FA+KLSLLSRNGFFACSS+   K      +  +  ++ +  +KK        E+P +YLS+C+ELETIA+ I ++  +W+  F+  L ++G+NV  +EK   I   N                         S+PVDLDT+   IS ++F+GGEK +ST  N + KLVLWSVT N  GQ CISG +LT+  + F EI P  I SV N+ +AVIA+KCCYEL+GPMY LS+RLA P A N++Y+WP  T+FT+ LP +WK F  AID LCKCSPLR  S    D 
Sbjct:  559 LLSYCHSEKLFTTLDCSYIFTDGIDIEFMGKYVKFLQEKPLELLGKFDGDNWLKFAIKLSLLSRNGFFACSSESFKKSLQTNPNSVLLKVLCKTPVKKIYSGTPKVEQPPHYLSVCKELETIANLIGKNG-NWLHGFEKLLQKYGQNVILNEKLKSIMDSN------------------------LSKPVDLDTT---ISLDDFNGGEKFSSTLVNFESKLVLWSVTVNTNGQLCISGFQLTDSNKLFNEIKPTPISSVTNNRRAVIANKCCYELVGPMYTLSLRLAYPEAVNMEYLWPFQTYFTSCLPSNWKAFILAIDTLCKCSPLRKESCEAADV 868
The following BLAST results are available for this feature:
BLAST of SMED30004541 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004541 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000056767.12.382e-13095.35SMESG000056767.1[more]
SMESG000016241.11.230e-11652.93SMESG000016241.1[more]
SMESG000016241.12.828e-11652.93SMESG000016241.1[more]
SMESG000016241.11.559e-10052.66SMESG000016241.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004541 ID=SMED30004541|Name=SMED30004541|organism=Schmidtea mediterranea sexual|type=transcript|length=2509bp
TTGAAATCAAAGTCTTTCTAAAAACTTTAATTTAATAAAATTTATATAAT
TAAAATGAGCTTTGTTTCGAAGTGGTTTCAAAAATGCCATGCTGCAAGAC
TTTCAAAATACAACCAAGAAGTAATCAATAAAGAACATTTTATAGATAAA
TATCCCAAGTTTCCATTTTCAACAAATTTTCTGAATGAAATAATGATTCT
TACATACGGCTATGAAAATTCAATTGCTGATGATGATAGTTTTCCTGTCT
CTCATATCCAATGGAATGAGAAAATTTTTTATGGATATAAGGAAATAAGC
TACCTCAAACTTCTAGATATGAAATGCGAATCTTCTCTAAAATCAGCCAA
TAGTAAAAATCTTGAAGATATTCATGTAGACAGAAACCACTCACCGAGTA
TTGACGAGTCGACTTTTTTCAATGCAAAGGAACCGTCGGGTTCAAATCTA
GCAGAAACAGTGAAAGATGTCCGATGTTGGTTTCCACTGTGGATTGACGG
GGTCTGTAAAATCGTTTGTCAACTCCATCAGCCACAAAGAACTGTTATTA
CCGGGGCAGTTCTCAGTTATTCCCATTCTCAGAAATTATTCACAACACTA
TATTGCAGCTATAAATTCACTGACGGAATCGACATTGAATTCATGGGGAA
ATATGTCAAATTCCTCCAGGAGATGCCTTTGGAATTATTGGGAAAGTTTG
ACGGCGACAATTGGCTGCAATTTGCTCTTAAACTTTCTTTGCTGAGTCGG
AATGGATTCTTTGCGTGTAGCAGTAAACCAATCACCAAATTGTCAGGCTG
CCGGCGCTCACAGTTTATCAATAATATTATAGGAAGAGCCCAATTGAAAA
AGCAATTGAAAAGGCCGCGCAATTCAGAACGTCCCGTGAATTACCTGAGT
ATTTGTGAAGAGCTAGAAACTATTGCTAGCGCTATTCAAGAATCAGCACG
CGATTGGGTGACAATGTTCCAGGCGTTCCTGCTGAGACACGGTGAAAATG
TGAGCGAAAAATTCGCCGGCATCGATTCACAGAATCCCGTTGCAAGGTTA
TTGATTATAGCAATTGCATGTTGTCCCGAGTTACCGTGATGCTGTCTCTC
TCTATTTCATTCTAGACCGGTGGACCTCGACACGAGCCAGGAATCGATTT
CCTATGAGAATTTCAGTGGCGGGGAGAAATGCAACTCAACATTCACAAAT
CTCGATGGGAAACTCGTGCTCTGGTCGGTGACAAGGAATCCGATTGGCCA
GTTCTGCATTTCCGGAATGAAACTAACAAATGGCCGTCAGCGGTTCATTG
AGATCGATCCGGTTGCGATCGGGTCGGTGATGAACAGTGGCAAGGCAGTG
ATTGCCGATAAATGCTGCTACGAATTGCTGGGCCCCATGTACCCGTTATC
GATCCGGCTGGCAGTTCCCAGCGCATCAAATATAGATTATATATGGCCCT
TGCTCACGCATTTCACCAATGGTCTCCCCCTGCATTGGAAATGTTTCGCT
CAGGCCATTGATGTGTTGTGCAAATGTTCTCCTCTCAGGTACTCATCTAT
TTCATTGGATGATTGTCAGAGGGTGTTGGGGAATTTGTCCCCCTGCAACC
TATCGTACCGCCGTACCATTTCAATATTCATTGTCCTACAGGCCGCACGA
GTCCTCATCGATGCTATTTCTGACTCCAATTCCAGCATCGAGATCCAGGC
GGAATTCTCAGTCCTCCAACTCACACACGATATCTGATACCAATACCATT
CTCATGATGCCCGATGTGAGACTCGATAGACTGCCAATCGTCGTGGAAAA
GGCAGTAGACAGATCCAGTGATGCAAATATCCATCAATCGCCAACAACAC
GGAGCATACTCGACATTTCTCCCGTGTTACCCCAGAGGCCTGTTGTCAGG
AACCACGAGGAACCGCCTGTTTCATCGGTCGCTGTGACCACTATTAACAC
CAGAAAACGGAGGACCGAATCAAGTAAATCATTTTTTTCGCTATATTTCA
TTGGTTTTTTCGTTATTAAGGCCATTCAGTCGGAATGTCAAGCCAGCTAC
CAATGAAAAAAGGACAGCTACAAAATCAAAGAGTTAAAAAGAAAAAATGT
AGCTTTGCAATTCCTTTGATATCAATGAGTAGATCGGATTTCAGTCGATC
GCGATCAGTTTCAGGGCCAGCAATTCGAGGTGTGGAAAGAGAGCCGCAAT
TGAATTATAGGAAAAAAGCTGTGAAAAATTCGCAACAACATAGAGCGAGA
TCCAGTTTGTTTGATGTGGACTTTACACCGAGCAGGCGGCACAGTCTCGA
CTACTCCCAGTACATCGTGGAATATATGTGCCTCAATGACATGGTGCAGA
CCACCAGGAGCGGCAGGAAGAGTTTGCCCCCGCTTGACCCCCGTTTCGAC
TTGGAATATAAAATAGAAAATGAAAAAGTATTCCTCCTATCAAATGGCGA
AAAATTTGAGTTGGCGAGTCGCGTTGCACGTTCTCACAGATGATAATTTT
ATTCCCATA
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protein sequence of SMED30004541-orf-1

>SMED30004541-orf-1 ID=SMED30004541-orf-1|Name=SMED30004541-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=153bp
MSSQLPMKKGQLQNQRVKKKKCSFAIPLISMSRSDFSRSRSVSGPAIRGV
EREPQLNYRKKAVKNSQQHRARSSLFDVDFTPSRRHSLDYSQYIVEYMCL
NDMVQTTRSGRKSLPPLDPRFDLEYKIENEKVFLLSNGEKFELASRVARS
HR*
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protein sequence of SMED30004541-orf-2

>SMED30004541-orf-2 ID=SMED30004541-orf-2|Name=SMED30004541-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=345bp
MSFVSKWFQKCHAARLSKYNQEVINKEHFIDKYPKFPFSTNFLNEIMILT
YGYENSIADDDSFPVSHIQWNEKIFYGYKEISYLKLLDMKCESSLKSANS
KNLEDIHVDRNHSPSIDESTFFNAKEPSGSNLAETVKDVRCWFPLWIDGV
CKIVCQLHQPQRTVITGAVLSYSHSQKLFTTLYCSYKFTDGIDIEFMGKY
VKFLQEMPLELLGKFDGDNWLQFALKLSLLSRNGFFACSSKPITKLSGCR
RSQFINNIIGRAQLKKQLKRPRNSERPVNYLSICEELETIASAIQESARD
WVTMFQAFLLRHGENVSEKFAGIDSQNPVARLLIIAIACCPELP*
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protein sequence of SMED30004541-orf-3

>SMED30004541-orf-3 ID=SMED30004541-orf-3|Name=SMED30004541-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=157bp
MKLTNGRQRFIEIDPVAIGSVMNSGKAVIADKCCYELLGPMYPLSIRLAV
PSASNIDYIWPLLTHFTNGLPLHWKCFAQAIDVLCKCSPLRYSSISLDDC
QRVLGNLSPCNLSYRRTISIFIVLQAARVLIDAISDSNSSIEIQAEFSVL
QLTHDI*
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protein sequence of SMED30004541-orf-4

>SMED30004541-orf-4 ID=SMED30004541-orf-4|Name=SMED30004541-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=196bp
MVSPCIGNVSLRPLMCCANVLLSGTHLFHWMIVRGCWGICPPATYRTAVP
FQYSLSYRPHESSSMLFLTPIPASRSRRNSQSSNSHTISDTNTILMMPDV
RLDRLPIVVEKAVDRSSDANIHQSPTTRSILDISPVLPQRPVVRNHEEPP
VSSVAVTTINTRKRRTESSKSFFSLYFIGFFVIKAIQSECQASYQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000099neuron
PLANA:0000429neoblast
PLANA:0002109X1 cell