Snail-1

Overview
NameSnail-1
Smed IDSMED30004536
Length (bp)824
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Snail-1 (SMED30004536) t-SNE clustered cells

Violin plots show distribution of expression levels for Snail-1 (SMED30004536) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Snail-1 (SMED30004536) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Snail-1 (SMED30004536) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30004536

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30004536h1SMcG0011242 dd_Smed_v4_15506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004536h1SMcG0011242 dd_Smed_v4_15506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30004536h1SMcG0011242 dd_Smed_v4_15506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30004536h1SMcG0011242 dd_Smed_v4_15506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
prepharyngeal regionSMED30004536h1SMcG0011242 dd_Smed_v6_15506_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
whole organismSMED30004536h1SMcG0011242 KY964486smed_ncbi_20240424PMID:28893948
Abnave et al., 2017
whole organism asexual adult fluorescence in situ hybridization evidence
neoblastSMED30004536h1SMcG0011242 KY964486smed_ncbi_20240424PMID:28893948
Abnave et al., 2017
whole organism asexual adult fluorescence in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Snail-1 vs. Ensembl Human
Match: SNAI2 (snail family transcriptional repressor 2 [Source:HGNC Symbol;Acc:HGNC:11094])

HSP 1 Score: 140.584 bits (353), Expect = 9.899e-40
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C
Sbjct:  158 SFSCKYCDKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGC 264          
BLAST of Snail-1 vs. Ensembl Human
Match: SNAI1 (snail family transcriptional repressor 1 [Source:HGNC Symbol;Acc:HGNC:11128])

HSP 1 Score: 140.198 bits (352), Expect = 1.072e-39
Identity = 64/105 (60.95%), Postives = 76/105 (72.38%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCA 767
            C  C K++ S  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C R+FSR SLL KH    C+
Sbjct:  156 CKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSLLHKHQESGCS 260          
BLAST of Snail-1 vs. Ensembl Human
Match: SNAI3 (snail family transcriptional repressor 3 [Source:HGNC Symbol;Acc:HGNC:18411])

HSP 1 Score: 137.502 bits (345), Expect = 2.264e-38
Identity = 63/104 (60.58%), Postives = 76/104 (73.08%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            C  C K++ S  ALKMH RTH  PC C  CGKAFSR WLL GH+R HTGE+PY C+ C RAFADRSNLRAH+QTH+  K+Y+C +C ++FSR SLL +H    C
Sbjct:  185 CKYCDKEYTSLGALKMHIRTHTLPCTCKICGKAFSRPWLLQGHVRTHTGEKPYACSHCSRAFADRSNLRAHLQTHSDAKKYRCRRCTKTFSRMSLLARHEESGC 288          
BLAST of Snail-1 vs. Ensembl Human
Match: SCRT1 (scratch family transcriptional repressor 1 [Source:HGNC Symbol;Acc:HGNC:15950])

HSP 1 Score: 126.331 bits (316), Expect = 1.459e-33
Identity = 59/114 (51.75%), Postives = 76/114 (66.67%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            + + ++  CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K +QC +C +SF+ +S L KH    C K
Sbjct:  216 LDSQLARRCPTCGKVYVSMPAMAMHLLTHDLRHKCGVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHFQCKRCKKSFALKSYLNKHYESACFK 329          
BLAST of Snail-1 vs. Ensembl Human
Match: SCRT1 (scratch family transcriptional repressor 1 [Source:HGNC Symbol;Acc:HGNC:15950])

HSP 1 Score: 126.331 bits (316), Expect = 1.459e-33
Identity = 59/114 (51.75%), Postives = 76/114 (66.67%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            + + ++  CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K +QC +C +SF+ +S L KH    C K
Sbjct:  216 LDSQLARRCPTCGKVYVSMPAMAMHLLTHDLRHKCGVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHFQCKRCKKSFALKSYLNKHYESACFK 329          
BLAST of Snail-1 vs. Ensembl Celegans
Match: snai-1 (SNAIl family zinc finger 2 transcription factor homolog [Source:UniProtKB/TrEMBL;Acc:O45103])

HSP 1 Score: 130.954 bits (328), Expect = 2.393e-37
Identity = 59/110 (53.64%), Postives = 78/110 (70.91%), Query Frame = 3
Query:  435 NSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
              +   CP C K + S  ALKMH +TH+ PC+C  CGK+FSR WLL GHLR HTGE+P+ C  C R FADRSNLRAH+QTH+  K+++C +C +SF+R  + ++H  QCC
Sbjct:   66 GKLQQSCPHCKKVYRSPGALKMHLKTHSLPCVCNDCGKSFSRPWLLKGHLRTHTGEKPFGCEFCGRCFADRSNLRAHLQTHSGEKKHRCSRCGQSFARVQVRQRHE-QCC 174          
BLAST of Snail-1 vs. Ensembl Celegans
Match: ces-1 (CES-1; CEll death Specification [Source:UniProtKB/TrEMBL;Acc:G5ECN8])

HSP 1 Score: 120.168 bits (300), Expect = 2.065e-32
Identity = 57/104 (54.81%), Postives = 70/104 (67.31%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQC 761
            +CP C + + S  AL MH  THN    C  CGK FSR+WLL GHLR HTG RP+ C  C ++FADRSNLRAHM THT  KR++CDKC R F+ ++ L +H   C
Sbjct:  166 QCPHCDRVYVSMPALSMHILTHNASHECNVCGKRFSRLWLLQGHLRSHTGLRPFSCAHCGKSFADRSNLRAHMLTHTGDKRFECDKCGRRFALRAYLNRHLETC 269          
BLAST of Snail-1 vs. Ensembl Celegans
Match: scrt-1 (SCRaTch (Zinc finger transcriptional repressor) homolog [Source:UniProtKB/TrEMBL;Acc:O01830])

HSP 1 Score: 102.834 bits (255), Expect = 1.204e-26
Identity = 47/90 (52.22%), Postives = 61/90 (67.78%), Query Frame = 3
Query:  510 THNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVD 779
            T +P C C  CGK FSR WLL GHLR HTGE+P+ C  C + FAD+SNLRAH+QTH+  K ++C +C +SF+ +S L KH    C   +D
Sbjct:   89 TCSPVCQCKVCGKRFSRQWLLQGHLRTHTGEKPFQCEICSKRFADKSNLRAHIQTHSGTKPHKCPRCGKSFALKSYLSKHEESKCLPRMD 178          
BLAST of Snail-1 vs. Ensembl Celegans
Match: che-1 (Transcription factor che-1 [Source:UniProtKB/Swiss-Prot;Acc:Q966L8])

HSP 1 Score: 83.5741 bits (205), Expect = 3.587e-19
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGK  + ++ L  H+R H    P +CP C + FS+   L  H R HTGERPY C +C++AF D S L  H++THT  K Y C  C   F++   L +H
Sbjct:  109 CQTCGKAFSQAANLTAHKRIHTGEKPFMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLRTHTGHKPYVCSICMMKFTQSGNLHRH 209          

HSP 2 Score: 75.485 bits (184), Expect = 3.515e-16
Identity = 35/76 (46.05%), Postives = 48/76 (63.16%), Query Frame = 3
Query:  522 PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            P  C  CGKAFS+   L+ H RIHTGE+P++C  C R F+  S+L  H +THT  + Y C +C ++F+  S L KH
Sbjct:  106 PFRCQTCGKAFSQAANLTAHKRIHTGEKPFMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKH 181          

HSP 3 Score: 72.0182 bits (175), Expect = 7.400e-15
Identity = 36/77 (46.75%), Postives = 46/77 (59.74%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTH 677
            CP+C +  + SS+L  H+RTH    P  C QC KAF+    L+ HLR HTG +PYVC+ C   F    NL  HM+TH
Sbjct:  137 CPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLRTHTGHKPYVCSICMMKFTQSGNLHRHMKTH 213          
BLAST of Snail-1 vs. Ensembl Celegans
Match: che-1 (Transcription factor che-1 [Source:UniProtKB/Swiss-Prot;Acc:Q966L8])

HSP 1 Score: 84.3445 bits (207), Expect = 6.535e-19
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGK  + ++ L  H+R H    P +CP C + FS+   L  H R HTGERPY C +C++AF D S L  H++THT  K Y C  C   F++   L +H
Sbjct:  168 CQTCGKAFSQAANLTAHKRIHTGEKPFMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLRTHTGHKPYVCSICMMKFTQSGNLHRH 268          

HSP 2 Score: 75.8702 bits (185), Expect = 7.608e-16
Identity = 35/76 (46.05%), Postives = 48/76 (63.16%), Query Frame = 3
Query:  522 PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            P  C  CGKAFS+   L+ H RIHTGE+P++C  C R F+  S+L  H +THT  + Y C +C ++F+  S L KH
Sbjct:  165 PFRCQTCGKAFSQAANLTAHKRIHTGEKPFMCPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKH 240          

HSP 3 Score: 72.0182 bits (175), Expect = 1.533e-14
Identity = 36/77 (46.75%), Postives = 46/77 (59.74%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTH 677
            CP+C +  + SS+L  H+RTH    P  C QC KAF+    L+ HLR HTG +PYVC+ C   F    NL  HM+TH
Sbjct:  196 CPVCNRPFSQSSSLVTHRRTHTGERPYPCAQCEKAFTDSSTLTKHLRTHTGHKPYVCSICMMKFTQSGNLHRHMKTH 272          
BLAST of Snail-1 vs. Ensembl Fly
Match: sna (gene:FBgn0003448 transcript:FBtr0080739)

HSP 1 Score: 140.198 bits (352), Expect = 9.023e-39
Identity = 66/107 (61.68%), Postives = 75/107 (70.09%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            +H C  CGK + +  ALKMH RTH  PC CP CGKAFSR WLL GH+R HTGE+P+ C  C R+FADRSNLRAH QTH   K+Y C  C +SFSR SLL KHS   C
Sbjct:  279 THSCEECGKLYTTIGALKMHIRTHTLPCKCPICGKAFSRPWLLQGHIRTHTGEKPFQCPDCPRSFADRSNLRAHQQTHVDVKKYACQVCHKSFSRMSLLNKHSSSNC 385          

HSP 2 Score: 47.7506 bits (112), Expect = 5.246e-6
Identity = 26/80 (32.50%), Postives = 40/80 (50.00%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTT 683
            +CP+CGK  +    L+ H RTH    P  CP C ++F+    L  H + H   + Y C  C ++F+  S L  H  ++ T
Sbjct:  307 KCPICGKAFSRPWLLQGHIRTHTGEKPFQCPDCPRSFADRSNLRAHQQTHVDVKKYACQVCHKSFSRMSLLNKHSSSNCT 386          
BLAST of Snail-1 vs. Ensembl Fly
Match: wor (gene:FBgn0001983 transcript:FBtr0080740)

HSP 1 Score: 140.198 bits (352), Expect = 8.763e-38
Identity = 66/104 (63.46%), Postives = 73/104 (70.19%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            C  C K + S  ALKMH RTH  PC CP CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAHMQTH+  K+Y C  C +SFSR SLL KH    C
Sbjct:  382 CKNCDKTYVSLGALKMHIRTHTLPCKCPICGKAFSRPWLLQGHIRTHTGEKPFSCQHCNRAFADRSNLRAHMQTHSDVKKYSCPTCTKSFSRMSLLAKHLQSGC 485          
BLAST of Snail-1 vs. Ensembl Fly
Match: esg (gene:FBgn0001981 transcript:FBtr0080734)

HSP 1 Score: 134.806 bits (338), Expect = 3.281e-36
Identity = 64/103 (62.14%), Postives = 73/103 (70.87%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHS 752
            S  C  C K + S  ALKMH RTH  PC C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+Y C  C ++FSR SLL KHS
Sbjct:  343 SFSCKDCDKTYVSLGALKMHIRTHTLPCKCNLCGKAFSRPWLLQGHIRTHTGEKPFSCQHCHRAFADRSNLRAHLQTHSDIKKYSCTSCSKTFSRMSLLTKHS 445          
BLAST of Snail-1 vs. Ensembl Fly
Match: Kah (gene:FBgn0035144 transcript:FBtr0072563)

HSP 1 Score: 124.405 bits (311), Expect = 1.417e-32
Identity = 57/114 (50.00%), Postives = 75/114 (65.79%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            I++  +  CP C K + S  A  MH RTHN  C C  CGK FSR WLL GH+R HTGE+P+ C  C++AFAD+SNLRAH+QTH+  K + C +C ++F+ +S L KH    C K
Sbjct:  144 IMDKKARHCPYCEKVYVSMPAYSMHVRTHNQGCECQFCGKRFSRPWLLQGHIRTHTGEKPFKCGVCEKAFADKSNLRAHIQTHSNTKPHTCARCGKAFALKSYLYKHEESSCMK 257          
BLAST of Snail-1 vs. Ensembl Fly
Match: scrt (gene:FBgn0004880 transcript:FBtr0333136)

HSP 1 Score: 121.324 bits (303), Expect = 8.833e-31
Identity = 58/107 (54.21%), Postives = 70/107 (65.42%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            +C  CGK + S  AL MH  TH     C  CGK FSR WLL GHLR HTGE+PY C  C +AFADRSNLRAHMQTH+  K ++C +C ++F+ +S L KH    C K
Sbjct:  499 KCHTCGKAYVSMPALAMHLLTHKLSHSCGVCGKLFSRPWLLQGHLRSHTGEKPYGCAHCGKAFADRSNLRAHMQTHSVDKNFECKRCHKTFALKSYLNKHLESACLK 605          
BLAST of Snail-1 vs. Ensembl Zebrafish
Match: snai1a (snail family zinc finger 1a [Source:ZFIN;Acc:ZDB-GENE-990415-255])

HSP 1 Score: 148.673 bits (374), Expect = 3.697e-43
Identity = 70/111 (63.06%), Postives = 81/111 (72.97%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQ-CCAKLVDV 782
            C  C K++NS  ALKMH R+H  PC+CP CGKAFSR WLL GH+R HTGERP+ C  C RAFADRSNLRAH+QTH   K+YQC  C R+FSR SLL+KHS   CC    +V
Sbjct:  149 CKHCPKEYNSLGALKMHIRSHTLPCVCPTCGKAFSRPWLLRGHIRTHTGERPFSCPHCNRAFADRSNLRAHLQTHADVKKYQCSTCSRTFSRMSLLQKHSAAGCCPSTANV 259          
BLAST of Snail-1 vs. Ensembl Zebrafish
Match: snai1a (snail family zinc finger 1a [Source:ZFIN;Acc:ZDB-GENE-990415-255])

HSP 1 Score: 146.747 bits (369), Expect = 1.894e-42
Identity = 67/100 (67.00%), Postives = 77/100 (77.00%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHS 752
            C  C K++NS  ALKMH R+H  PC+CP CGKAFSR WLL GH+R HTGERP+ C  C RAFADRSNLRAH+QTH   K+YQC  C R+FSR SLL+KHS
Sbjct:  149 CKHCPKEYNSLGALKMHIRSHTLPCVCPTCGKAFSRPWLLRGHIRTHTGERPFSCPHCNRAFADRSNLRAHLQTHADVKKYQCSTCSRTFSRMSLLQKHS 248          
BLAST of Snail-1 vs. Ensembl Zebrafish
Match: snai1b (snail family zinc finger 1b [Source:ZFIN;Acc:ZDB-GENE-980526-514])

HSP 1 Score: 144.05 bits (362), Expect = 2.090e-41
Identity = 69/108 (63.89%), Postives = 80/108 (74.07%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQ-CCAKL 773
            C  C K++NS  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGERP+ C  C RAFADRSNLRAH+QTH+  K+YQC  C R+FSR SLL KH+L  CC  L
Sbjct:  149 CKHCPKEYNSLGALKMHIRSHTLPCVCSTCGKAFSRPWLLRGHIRTHTGERPFSCPHCNRAFADRSNLRAHLQTHSEVKKYQCGSCSRTFSRMSLLHKHTLSGCCPAL 256          
BLAST of Snail-1 vs. Ensembl Zebrafish
Match: snai2 (snail family zinc finger 2 [Source:ZFIN;Acc:ZDB-GENE-030326-6])

HSP 1 Score: 140.969 bits (354), Expect = 2.909e-40
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C
Sbjct:  147 SFSCKYCEKEYVSLGALKMHIRTHTLPCVCKMCGKAFSRPWLLQGHIRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGC 253          
BLAST of Snail-1 vs. Ensembl Zebrafish
Match: snai3 (snail family zinc finger 3 [Source:ZFIN;Acc:ZDB-GENE-061013-757])

HSP 1 Score: 135.961 bits (341), Expect = 4.768e-38
Identity = 64/107 (59.81%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKL 773
            C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C  +
Sbjct:  176 CKYCEKEYVSLGALKMHIRTHTLPCVCKLCGKAFSRPWLLQGHIRTHTGEKPFTCPHCSRAFADRSNLRAHLQTHSEIKKYQCRNCFKTFSRISLLTKHEEAGCCPM 282          
BLAST of Snail-1 vs. Ensembl Xenopus
Match: snai1 (snail family zinc finger 1 [Source:NCBI gene;Acc:394880])

HSP 1 Score: 141.354 bits (355), Expect = 1.825e-39
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C R+FSR SLL KH    C
Sbjct:  226 SFSCKYCEKEYVSLGALKMHIRSHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCTHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSRTFSRMSLLHKHEETGC 332          
BLAST of Snail-1 vs. Ensembl Xenopus
Match: srpk2 (SRSF protein kinase 2 [Source:Xenbase;Acc:XB-GENE-990797])

HSP 1 Score: 139.428 bits (350), Expect = 2.477e-39
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C
Sbjct:  156 SFSCKYCEKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGC 262          
BLAST of Snail-1 vs. Ensembl Xenopus
Match: SNAI3 (snail family zinc finger 3 [Source:NCBI gene;Acc:100488776])

HSP 1 Score: 134.42 bits (337), Expect = 2.370e-37
Identity = 64/104 (61.54%), Postives = 73/104 (70.19%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            C  C K ++S  ALKMH RTH  PC C  CGKAFSR WLL GH+R HTGE+P+ C  C R FADRSNLRAH+QTH+  KRYQC  C ++FSR SLL KH    C
Sbjct:  171 CQHCAKAYSSLGALKMHIRTHTLPCTCRICGKAFSRPWLLQGHIRTHTGEKPFSCFHCGRGFADRSNLRAHLQTHSEIKRYQCPGCGKTFSRISLLAKHRDGSC 274          
BLAST of Snail-1 vs. Ensembl Xenopus
Match: SCRT1 (scratch family transcriptional repressor 1 [Source:HGNC Symbol;Acc:HGNC:15950])

HSP 1 Score: 123.635 bits (309), Expect = 1.591e-33
Identity = 56/107 (52.34%), Postives = 75/107 (70.09%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            + + ++ +CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K Y+C +C ++F+ +S L KH
Sbjct:  132 LDSKLAKKCPTCGKVYVSMPAMAMHLLTHDLKHKCETCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHYKCKRCSKTFALKSYLNKH 238          
BLAST of Snail-1 vs. Ensembl Xenopus
Match: ENSXETT00000046689.1 (zinc finger protein 84 [Source:NCBI gene;Acc:496795])

HSP 1 Score: 99.7525 bits (247), Expect = 4.106e-23
Identity = 48/103 (46.60%), Postives = 65/103 (63.11%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSL 755
            C +CGK  + SS L  HQ  H    P +C +CGKAF+R   L+ H R HTGE+PY C+ C + F D+SNL +H +TH   K+Y+C +C R F++ S L KH L
Sbjct:  420 CNVCGKSFSCSSTLIKHQIIHTGERPYVCLECGKAFTRGSHLAIHQRTHTGEKPYSCDDCGKGFTDKSNLASHQRTHKGVKQYECKECGRKFTQSSTLLKHQL 522          

HSP 2 Score: 81.2629 bits (199), Expect = 8.291e-17
Identity = 45/101 (44.55%), Postives = 56/101 (55.45%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGK    +S L +H + H  + P  C  CGK+FS    L  H  IHTGERPYVC  C +AF   S+L  H +THT  K Y CD C + F+ +S L  H
Sbjct:  392 CNECGKSFFCNSRLVLHMKAHTGDRPFGCNVCGKSFSCSSTLIKHQIIHTGERPYVCLECGKAFTRGSHLAIHQRTHTGEKPYSCDDCGKGFTDKSNLASH 492          

HSP 3 Score: 80.1073 bits (196), Expect = 1.946e-16
Identity = 42/103 (40.78%), Postives = 56/103 (54.37%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            + C  CGK     S L  HQRTH       C +CG+ F++   L  H  IHTGE+P+ C+ C + F+  SNL  H++ HT  + Y C  C RSF+  S L KH
Sbjct:  474 YSCDDCGKGFTDKSNLASHQRTHKGVKQYECKECGRKFTQSSTLLKHQLIHTGEKPFACSECGKLFSRSSNLIIHLRMHTGERPYSCTVCERSFAHSSHLVKH 576          

HSP 4 Score: 80.1073 bits (196), Expect = 2.369e-16
Identity = 44/103 (42.72%), Postives = 56/103 (54.37%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSL 755
            C  CGK     S L  H RTH    P  C +CGK+F     L  H R HTGE+P+VCN C ++F   S L  HM+ HT  + + C+ C +SFS  S L KH +
Sbjct:  336 CSYCGKTFTDRSNLVSHLRTHTGEKPYSCIECGKSFPYRSALVLHKRTHTGEKPFVCNECGKSFFCNSRLVLHMKAHTGDRPFGCNVCGKSFSCSSTLIKHQI 438          

HSP 5 Score: 78.5666 bits (192), Expect = 6.217e-16
Identity = 41/94 (43.62%), Postives = 55/94 (58.51%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSF 722
            ++C  C K+   S+ L +H RTH    P IC  CGK F+    L  HLR HTGE+PY C  C ++F  RS L  H +THT  K + C++C +SF
Sbjct:  306 YKCTQCDKRFTRSANLVVHYRTHTGEKPYICSYCGKTFTDRSNLVSHLRTHTGEKPYSCIECGKSFPYRSALVLHKRTHTGEKPFVCNECGKSF 399          

HSP 6 Score: 77.411 bits (189), Expect = 1.910e-15
Identity = 36/73 (49.32%), Postives = 47/73 (64.38%), Query Frame = 3
Query:  531 CPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C QC K F+R   L  H R HTGE+PY+C+ C + F DRSNL +H++THT  K Y C +C +SF  +S L  H
Sbjct:  308 CTQCDKRFTRSANLVVHYRTHTGEKPYICSYCGKTFTDRSNLVSHLRTHTGEKPYSCIECGKSFPYRSALVLH 380          

HSP 7 Score: 76.2554 bits (186), Expect = 4.035e-15
Identity = 43/106 (40.57%), Postives = 62/106 (58.49%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAF---SRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            + C  CGK     SAL +H+RTH    P +C +CGK+F   SR+ L   H++ HTG+RP+ CN C ++F+  S L  H   HT  + Y C +C ++F+R S L  H
Sbjct:  362 YSCIECGKSFPYRSALVLHKRTHTGEKPFVCNECGKSFFCNSRLVL---HMKAHTGDRPFGCNVCGKSFSCSSTLIKHQIIHTGERPYVCLECGKAFTRGSHLAIH 464          

HSP 8 Score: 73.9442 bits (180), Expect = 2.310e-14
Identity = 39/79 (49.37%), Postives = 47/79 (59.49%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTH 677
            +EC  CG+K   SS L  HQ  H    P  C +CGK FSR   L  HLR+HTGERPY C  C+R+FA  S+L  H + H
Sbjct:  502 YECKECGRKFTQSSTLLKHQLIHTGEKPFACSECGKLFSRSSNLIIHLRMHTGERPYSCTVCERSFAHSSHLVKHQKLH 580          

HSP 9 Score: 73.559 bits (179), Expect = 3.715e-14
Identity = 41/101 (40.59%), Postives = 48/101 (47.52%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGK     S L +HQRTH    P  C  CGK F+    L+ H R H G + Y C  C R F   S L  H   HT  K + C +C + FSR S L  H
Sbjct:  448 CLECGKAFTRGSHLAIHQRTHTGEKPYSCDDCGKGFTDKSNLASHQRTHKGVKQYECKECGRKFTQSSTLLKHQLIHTGEKPFACSECGKLFSRSSNLIIH 548          

HSP 10 Score: 68.5514 bits (166), Expect = 1.790e-12
Identity = 32/73 (43.84%), Postives = 43/73 (58.90%), Query Frame = 3
Query:  531 CPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C +CGK F +   L  H + HTGE+PY CN C   FA++S L  H +TH+  K Y C  C ++FS+ S L  H
Sbjct:  173 CLECGKTFIQSSHLKSHQKTHTGEKPYACNECGARFAEKSTLVKHQRTHSGEKPYVCLICDKAFSQNSHLTMH 245          

HSP 11 Score: 68.1662 bits (165), Expect = 2.038e-12
Identity = 37/88 (42.05%), Postives = 49/88 (55.68%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKC 710
            C  CGK    SS LK HQ+TH    P  C +CG  F+    L  H R H+GE+PYVC  C +AF+  S+L  H +TH  ++   C +C
Sbjct:  173 CLECGKTFIQSSHLKSHQKTHTGEKPYACNECGARFAEKSTLVKHQRTHSGEKPYVCLICDKAFSQNSHLTMHQRTHIVKE--TCSEC 258          

HSP 12 Score: 51.6026 bits (122), Expect = 6.253e-7
Identity = 22/53 (41.51%), Postives = 32/53 (60.38%), Query Frame = 3
Query:  591 HTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            H G+R Y C  C + F   S+L++H +THT  K Y C++C   F+ +S L KH
Sbjct:  165 HKGDRRYTCLECGKTFIQSSHLKSHQKTHTGEKPYACNECGARFAEKSTLVKH 217          

HSP 13 Score: 51.2174 bits (121), Expect = 9.745e-7
Identity = 36/125 (28.80%), Postives = 54/125 (43.20%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCG--------------------------KAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C +C K  + +S L MHQRTH     C +CG                          +  +++  LS  + I   E+ Y C +C + F   +NL  H +THT  K Y C  C ++F+ +S L  H
Sbjct:  229 CLICDKAFSQNSHLTMHQRTHIVKETCSECGIIVTNISPYSKIHSAQLQSECSECSQRLVAKLDALSNRI-ISKREKIYKCTQCDKRFTRSANLVVHYRTHTGEKPYICSYCGKTFTDRSNLVSH 352          
BLAST of Snail-1 vs. Ensembl Mouse
Match: Snai1 (snail family zinc finger 1 [Source:MGI Symbol;Acc:MGI:98330])

HSP 1 Score: 142.124 bits (357), Expect = 1.335e-40
Identity = 65/105 (61.90%), Postives = 77/105 (73.33%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCA 767
            C  C K++ S  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGE+P+ C+ C RAFADRSNLRAH+QTH+  KRYQC  C R+FSR SLL KH    C+
Sbjct:  156 CKYCNKEYLSLGALKMHIRSHTLPCVCTTCGKAFSRPWLLQGHVRTHTGEKPFSCSHCNRAFADRSNLRAHLQTHSDVKRYQCQACARTFSRMSLLHKHQESGCS 260          
BLAST of Snail-1 vs. Ensembl Mouse
Match: Snai2 (snail family zinc finger 2 [Source:MGI Symbol;Acc:MGI:1096393])

HSP 1 Score: 140.198 bits (352), Expect = 8.534e-40
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C
Sbjct:  159 SFSCKYCDKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGC 265          
BLAST of Snail-1 vs. Ensembl Mouse
Match: Snai3 (snail family zinc finger 3 [Source:MGI Symbol;Acc:MGI:1353563])

HSP 1 Score: 140.584 bits (353), Expect = 1.032e-39
Identity = 65/104 (62.50%), Postives = 76/104 (73.08%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            C  C K++ S  ALKMH RTH  PCIC  CGKAFSR WLL GH+R HTGE+PY C+ C RAFADRSNLRAH+QTH   K+Y+C  CP++FSR SLL +H    C
Sbjct:  180 CRYCDKEYASLGALKMHIRTHTLPCICKVCGKAFSRPWLLQGHIRTHTGEKPYTCSHCSRAFADRSNLRAHLQTHVGTKKYRCAVCPKAFSRMSLLARHEEAGC 283          
BLAST of Snail-1 vs. Ensembl Mouse
Match: Scrt2 (scratch family zinc finger 2 [Source:MGI Symbol;Acc:MGI:2139287])

HSP 1 Score: 128.257 bits (321), Expect = 8.669e-35
Identity = 61/117 (52.14%), Postives = 78/117 (66.67%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVD 779
            + + ++ +CP CGK + S  AL MH  THN    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K Y+C +C +SF+ +S L KH    C K  +
Sbjct:  186 LDSQLARKCPTCGKAYVSMPALAMHVLTHNLRHKCGVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHYRCRQCDKSFALKSYLHKHCEAACVKAAE 302          
BLAST of Snail-1 vs. Ensembl Mouse
Match: Scrt1 (scratch family zinc finger 1 [Source:MGI Symbol;Acc:MGI:2176606])

HSP 1 Score: 126.331 bits (316), Expect = 9.505e-34
Identity = 61/123 (49.59%), Postives = 78/123 (63.41%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN*KTP 797
            + + ++  CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K +QC +C +SF+ +S L KH    C K       TP
Sbjct:  216 LDSQLARRCPTCGKVYVSMPAMAMHLLTHDLRHKCGVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHFQCKRCKKSFALKSYLNKHYESACFKGGASGPATP 338          
BLAST of Snail-1 vs. UniProt/SwissProt
Match: sp|P19382|SNAI1_XENLA (Protein snail homolog Sna OS=Xenopus laevis OX=8355 GN=snai1 PE=1 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 8.940e-40
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C R+FSR SLL KH    C
Sbjct:  149 SFSCKYCEKEYVSLGALKMHIRSHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCTHCNRAFADRSNLRAHLQTHSDVKKYQCKSCSRTFSRMSLLHKHEETGC 255          
BLAST of Snail-1 vs. UniProt/SwissProt
Match: sp|Q02085|SNAI1_MOUSE (Zinc finger protein SNAI1 OS=Mus musculus OX=10090 GN=Snai1 PE=1 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 9.339e-40
Identity = 65/105 (61.90%), Postives = 77/105 (73.33%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCA 767
            C  C K++ S  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGE+P+ C+ C RAFADRSNLRAH+QTH+  KRYQC  C R+FSR SLL KH    C+
Sbjct:  156 CKYCNKEYLSLGALKMHIRSHTLPCVCTTCGKAFSRPWLLQGHVRTHTGEKPFSCSHCNRAFADRSNLRAHLQTHSDVKRYQCQACARTFSRMSLLHKHQESGCS 260          
BLAST of Snail-1 vs. UniProt/SwissProt
Match: sp|Q3MHQ4|SNAI2_BOVIN (Zinc finger protein SNAI2 OS=Bos taurus OX=9913 GN=SNAI2 PE=2 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.308e-39
Identity = 67/109 (61.47%), Postives = 79/109 (72.48%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQ-CCA 767
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C+ C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    CCA
Sbjct:  158 SFSCKYCDKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCSHCSRAFADRSNLRAHLQTHSDVKKYQCKSCSKTFSRMSLLHKHEESGCCA 266          
BLAST of Snail-1 vs. UniProt/SwissProt
Match: sp|O43623|SNAI2_HUMAN (Zinc finger protein SNAI2 OS=Homo sapiens OX=9606 GN=SNAI2 PE=1 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 4.754e-39
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C
Sbjct:  158 SFSCKYCDKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGC 264          
BLAST of Snail-1 vs. UniProt/SwissProt
Match: sp|O95863|SNAI1_HUMAN (Zinc finger protein SNAI1 OS=Homo sapiens OX=9606 GN=SNAI1 PE=1 SV=2)

HSP 1 Score: 140.198 bits (352), Expect = 5.148e-39
Identity = 64/105 (60.95%), Postives = 76/105 (72.38%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCA 767
            C  C K++ S  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C R+FSR SLL KH    C+
Sbjct:  156 CKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQGHVRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSLLHKHQESGCS 260          
BLAST of Snail-1 vs. TrEMBL
Match: A0A221SAL3 (Snail-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 491.115 bits (1263), Expect = 9.096e-175
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 3
Query:   39 MESTFENYELVHQMVPNVAKFNVIQMIVSSIRNQMTNSTCKPIDIMNNNQKFEIIRRATYLYHYFVSQQERVPFRQPLYPAWNLKLINYPSPSTFPSLLNSENPNFLTTESIADTXXXXXXXXXXXXXIFIVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN 785
            MESTFENYELVHQMVPNVAKFNVIQMIVSSIRNQMTNSTCKPIDIMNNNQKFEIIRRATYLYHYFVSQQERVPFRQPLYPAWNLKLINYPSPSTFPSLLNSENPNFLTTESIADTDSGLPSSDSSGKSIFIVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN
Sbjct:    1 MESTFENYELVHQMVPNVAKFNVIQMIVSSIRNQMTNSTCKPIDIMNNNQKFEIIRRATYLYHYFVSQQERVPFRQPLYPAWNLKLINYPSPSTFPSLLNSENPNFLTTESIADTDSGLPSSDSSGKSIFIVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN 249          
BLAST of Snail-1 vs. TrEMBL
Match: A0A504YF64 (Protein escargot OS=Fasciola gigantica OX=46835 GN=FGIG_10337 PE=4 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 3.403e-41
Identity = 73/112 (65.18%), Postives = 81/112 (72.32%), Query Frame = 3
Query:  432 VNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCA 767
             +  ++EC LC K +N +SALKMH RTH  PC C  CGK+FSR WLL GH R HTGERPY CN C R+FADRSNLRAHMQTH   KRY C  CPRSFSR  LL KH +QC A
Sbjct:  483 TDGFTYECKLCHKTYNQASALKMHVRTHTLPCRCAHCGKSFSRKWLLKGHERTHTGERPYACNLCTRSFADRSNLRAHMQTHQREKRYSCPNCPRSFSRMGLLNKHMIQCRA 594          
BLAST of Snail-1 vs. TrEMBL
Match: A0A183B5V0 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS14585 PE=4 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 4.044e-41
Identity = 72/111 (64.86%), Postives = 80/111 (72.07%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQC 761
              + I++EC LC K +  +SALKMH RTH  PC C  CGK+FSR WLL GH R HTGERPY CN C R+FADRSNLRAHMQTH   KRY C  CPRSFSR  LL KH +QC
Sbjct:  492 TTDGITYECKLCQKTYTQASALKMHVRTHTLPCRCSHCGKSFSRKWLLKGHERTHTGERPYACNLCTRSFADRSNLRAHMQTHQREKRYSCPNCPRSFSRMGLLNKHMIQC 602          
BLAST of Snail-1 vs. TrEMBL
Match: A0A4E0RCV1 (Protein escargot OS=Fasciola hepatica OX=6192 GN=D915_004057 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 4.649e-41
Identity = 73/112 (65.18%), Postives = 80/112 (71.43%), Query Frame = 3
Query:  432 VNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCA 767
                ++EC LC K +N +SALKMH RTH  PC C  CGK+FSR WLL GH R HTGERPY CN C R+FADRSNLRAHMQTH   KRY C  CPRSFSR  LL KH +QC A
Sbjct:  454 TEGFTYECKLCHKTYNQASALKMHVRTHTLPCRCAHCGKSFSRKWLLKGHERTHTGERPYACNLCTRSFADRSNLRAHMQTHQREKRYSCPNCPRSFSRMGLLNKHMIQCRA 565          
BLAST of Snail-1 vs. TrEMBL
Match: A0A183QHE8 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 1.481e-40
Identity = 71/106 (66.98%), Postives = 78/106 (73.58%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQC 761
            S+ C LC K ++ +SALKMH RTH  PC C  CGK+FSR WLL GH R HTGERPY CN C R+FADRSNLRAHMQTH   KRY C  CPRSFSR  LL KH +QC
Sbjct:  484 SYACKLCSKVYSQASALKMHVRTHTLPCRCTHCGKSFSRKWLLKGHERTHTGERPYSCNVCSRSFADRSNLRAHMQTHQREKRYSCPYCPRSFSRMGLLNKHMIQC 589          
BLAST of Snail-1 vs. Ensembl Cavefish
Match: snai1b (protein snail homolog Sna-like [Source:NCBI gene;Acc:103022191])

HSP 1 Score: 144.436 bits (363), Expect = 1.501e-41
Identity = 67/108 (62.04%), Postives = 79/108 (73.15%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLV 776
            C  C K++NS  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGERP+ C  C RAFADRSNLRAH+QTH+  K+YQC  C R+FSR SLL KH++  C   V
Sbjct:  157 CKHCPKEYNSLGALKMHIRSHTLPCVCTTCGKAFSRPWLLRGHIRTHTGERPFSCPHCNRAFADRSNLRAHLQTHSEVKKYQCGTCSRTFSRMSLLHKHTVSSCCPSV 264          
BLAST of Snail-1 vs. Ensembl Cavefish
Match: snai2 (snail family transcriptional repressor 2 [Source:NCBI gene;Acc:111191588])

HSP 1 Score: 140.969 bits (354), Expect = 2.827e-40
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C
Sbjct:  149 SFSCKYCEKEYVSLGALKMHIRTHTLPCVCKMCGKAFSRPWLLQGHIRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGC 255          
BLAST of Snail-1 vs. Ensembl Cavefish
Match: ENSAMXT00000011311.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000047.1:155469:161795:1 gene:ENSAMXG00000011013.2 transcript:ENSAMXT00000011311.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 136.732 bits (343), Expect = 2.268e-38
Identity = 64/107 (59.81%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKL 773
            C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C  +
Sbjct:  171 CKYCDKEYVSLGALKMHIRTHTLPCVCKLCGKAFSRPWLLQGHIRTHTGEKPFSCPHCSRAFADRSNLRAHLQTHSDIKKYQCRSCSKTFSRISLLSKHEEAGCCPM 277          
BLAST of Snail-1 vs. Ensembl Cavefish
Match: SCRT1 (transcriptional repressor scratch 1-like [Source:NCBI gene;Acc:103031082])

HSP 1 Score: 125.561 bits (314), Expect = 3.272e-34
Identity = 57/114 (50.00%), Postives = 77/114 (67.54%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            + + ++ +CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K ++C +C ++F+ +S L KH    C K
Sbjct:  157 LDSQLAKKCPTCGKTYVSMPAMAMHLLTHDLKHKCDVCGKAFSRPWLLQGHMRSHTGEKPFACAHCGKAFADRSNLRAHMQTHSAFKHFRCKRCSKTFALKSYLNKHYESACFK 270          
BLAST of Snail-1 vs. Ensembl Cavefish
Match: scrt1a (transcriptional repressor scratch 1-like [Source:NCBI gene;Acc:103041010])

HSP 1 Score: 125.176 bits (313), Expect = 5.052e-34
Identity = 58/110 (52.73%), Postives = 75/110 (68.18%), Query Frame = 3
Query:  441 ISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            ++ +CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K Y+C +C ++F+ +S L KH    C K
Sbjct:  159 MAKKCPTCGKVYVSMPAMAMHLLTHDLKHKCDVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHYKCKRCNKTFALKSYLNKHYESACFK 268          
BLAST of Snail-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007831.1 (pep scaffold:Pmarinus_7.0:GL477655:47469:47963:1 gene:ENSPMAG00000007084.1 transcript:ENSPMAT00000007831.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.2117 bits (243), Expect = 2.452e-26
Identity = 46/100 (46.00%), Postives = 64/100 (64.00%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            +C +C K    SS L +H RTH  P  CP CGKAF++   L  H R HTGE+P+ C+ C++AF+  SNL+ H +THT  K ++C  C ++F+  S LRKH
Sbjct:    7 KCTVCEKAFAQSSYLTIHLRTHTKPFRCPLCGKAFAKSSDLQSHQRTHTGEKPFKCSVCEKAFSQSSNLKKHQRTHTGEKPFKCTVCEKAFTESSPLRKH 106          

HSP 2 Score: 65.855 bits (159), Expect = 3.955e-14
Identity = 34/80 (42.50%), Postives = 50/80 (62.50%), Query Frame = 3
Query:  510 THNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            T   P  C  C KAF++   L+ HLR HT  +P+ C  C +AFA  S+L++H +THT  K ++C  C ++FS+ S L+KH
Sbjct:    1 TGEKPFKCTVCEKAFAQSSYLTIHLRTHT--KPFRCPLCGKAFAKSSDLQSHQRTHTGEKPFKCSVCEKAFSQSSNLKKH 78          
BLAST of Snail-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000467.1 (pep scaffold:Pmarinus_7.0:GL480927:176958:178556:1 gene:ENSPMAG00000000421.1 transcript:ENSPMAT00000000467.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.0485 bits (227), Expect = 1.120e-21
Identity = 48/106 (45.28%), Postives = 61/106 (57.55%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPC-------ICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGK+ +  S LK HQR H  P        +C  CGK F+++  L  H RIHTGERPYVC  C RAFA  S L+ H +TH   K Y C +C   F++ S++ KH
Sbjct:  266 CEKCGKRFSDVSNLKAHQRIHADPFSKMAYMFVCKDCGKRFAKVTTLKNHRRIHTGERPYVCLECGRAFAQNSTLKNHQKTH-GEKSYVCSECGERFTKISVMNKH 370          

HSP 2 Score: 85.1149 bits (209), Expect = 3.043e-19
Identity = 47/98 (47.96%), Postives = 56/98 (57.14%), Query Frame = 3
Query:  486 SALKMHQRTHN--PPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFAD--------RSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            S L+ HQRTH+   P  C  CGK FS    L  H RIHTGERPY+C+ C R FA          SNL+ H +THT  K Y C+KC + FS  S L+ H
Sbjct:  185 SNLRRHQRTHSGEKPYACEDCGKTFSLTSTLKNHQRIHTGERPYICSHCGRGFAQISSLTVHRISNLKNHQRTHTGEKPYSCEKCGKRFSDVSNLKAH 282          

HSP 3 Score: 82.0333 bits (201), Expect = 3.766e-18
Identity = 44/105 (41.90%), Postives = 59/105 (56.19%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADR----SNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGKK  S S LK H+R H    P +C +CG+ F+ +  L  H R H+ E PY C  C+ AF  +    SNLR H +TH+  K Y C+ C ++FS  S L+ H
Sbjct:  114 CKACGKKFASKSLLKRHRRRHAFEMPRVCTECGRGFASISRLLVHQRTHSDEMPYACGECEAAFPLKSMLMSNLRRHQRTHSGEKPYACEDCGKTFSLTSTLKNH 218          

HSP 4 Score: 74.7146 bits (182), Expect = 1.140e-15
Identity = 43/114 (37.72%), Postives = 61/114 (53.51%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFS--------RMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHT---TRKRYQ--CDKCPRSFSRQSLLRKH 749
            C  CGK  + +S LK HQR H    P IC  CG+ F+        R+  L  H R HTGE+PY C +C + F+D SNL+AH + H    ++  Y   C  C + F++ + L+ H
Sbjct:  202 CEDCGKTFSLTSTLKNHQRIHTGERPYICSHCGRGFAQISSLTVHRISNLKNHQRTHTGEKPYSCEKCGKRFSDVSNLKAHQRIHADPFSKMAYMFVCKDCGKRFAKVTTLKNH 315          

HSP 5 Score: 68.5514 bits (166), Expect = 1.459e-13
Identity = 40/107 (37.38%), Postives = 55/107 (51.40%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSG----HLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSL 755
            C  CG+   S S L +HQRTH+   P  C +C  AF    +L      H R H+GE+PY C  C + F+  S L+ H + HT  + Y C  C R F++ S L  H +
Sbjct:  142 CTECGRGFASISRLLVHQRTHSDEMPYACGECEAAFPLKSMLMSNLRRHQRTHSGEKPYACEDCGKTFSLTSTLKNHQRIHTGERPYICSHCGRGFAQISSLTVHRI 248          
BLAST of Snail-1 vs. Ensembl Sea Lamprey
Match: zgc:113030 (zgc:113030 [Source:ZFIN;Acc:ZDB-GENE-050522-528])

HSP 1 Score: 87.8113 bits (216), Expect = 4.063e-21
Identity = 48/101 (47.52%), Postives = 60/101 (59.41%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGK    +S LK H RTH+   P +C  CGKAFS    L  H + HTGERP++C  C  AFA +S+L+ H  THTT K   CDKC +SF  ++ L  H
Sbjct:    6 CDECGKGFFYTSHLKTHMRTHSGERPFVCQDCGKAFSTNSNLKHHQK-HTGERPFICKLCGTAFAHKSSLKNHQNTHTTEKSLTCDKCGKSFVNRANLNAH 105          

HSP 2 Score: 78.5666 bits (192), Expect = 1.152e-17
Identity = 42/97 (43.30%), Postives = 57/97 (58.76%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWL---LSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSF 722
            H C  CGKK    S LK+HQ+TH  +   +C +CGK F  ++L   L  H + H+GER YVC  C +AF   S+L+ H Q H  ++ Y CD+C  SF
Sbjct:  115 HACEQCGKKFMMISYLKIHQKTHTLDGKHVCDECGKKF--IYLSSNLKVHQKGHSGERAYVCTECAKAFICMSHLKEHQQMHAGQRTYVCDQCGSSF 209          

HSP 3 Score: 75.8702 bits (185), Expect = 1.071e-16
Identity = 38/100 (38.00%), Postives = 57/100 (57.00%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQR-THNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  CGK  +++S LK HQ+ T   P IC  CG AF+    L  H   HT E+   C++C ++F +R+NL AH++ H   K + C++C + F   S L+ H
Sbjct:   34 CQDCGKAFSTNSNLKHHQKHTGERPFICKLCGTAFAHKSSLKNHQNTHTTEKSLTCDKCGKSFVNRANLNAHIKRHAAEKEHACEQCGKKFMMISYLKIH 133          

HSP 4 Score: 67.3958 bits (163), Expect = 9.724e-14
Identity = 31/74 (41.89%), Postives = 45/74 (60.81%), Query Frame = 3
Query:  528 ICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            +C +CGK F     L  H+R H+GERP+VC  C +AF+  SNL+ H Q HT  + + C  C  +F+ +S L+ H
Sbjct:    5 MCDECGKGFFYTSHLKTHMRTHSGERPFVCQDCGKAFSTNSNLK-HHQKHTGERPFICKLCGTAFAHKSSLKNH 77          

HSP 5 Score: 66.6254 bits (161), Expect = 1.740e-13
Identity = 33/92 (35.87%), Postives = 49/92 (53.26%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSF 722
            C LCG      S+LK HQ TH       C +CGK+F     L+ H++ H  E+ + C +C + F   S L+ H +THT   ++ CD+C + F
Sbjct:   61 CKLCGTAFAHKSSLKNHQNTHTTEKSLTCDKCGKSFVNRANLNAHIKRHAAEKEHACEQCGKKFMMISYLKIHQKTHTLDGKHVCDECGKKF 152          

HSP 6 Score: 53.1434 bits (126), Expect = 1.148e-8
Identity = 30/71 (42.25%), Postives = 41/71 (57.75%), Query Frame = 3
Query:  447 HECPLCGKKH-NSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRS 650
            H C  CGKK    SS LK+HQ+ H+     +C +C KAF  M  L  H ++H G+R YVC++C  +F   S
Sbjct:  143 HVCDECGKKFIYLSSNLKVHQKGHSGERAYVCTECAKAFICMSHLKEHQQMHAGQRTYVCDQCGSSFLSNS 213          

HSP 7 Score: 44.669 bits (104), Expect = 6.671e-6
Identity = 18/50 (36.00%), Postives = 31/50 (62.00%), Query Frame = 3
Query:  600 ERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            ER ++C+ C + F   S+L+ HM+TH+  + + C  C ++FS  S L+ H
Sbjct:    1 ERTFMCDECGKGFFYTSHLKTHMRTHSGERPFVCQDCGKAFSTNSNLKHH 50          
BLAST of Snail-1 vs. Ensembl Sea Lamprey
Match: ZNF436 (zinc finger protein 436 [Source:HGNC Symbol;Acc:HGNC:20814])

HSP 1 Score: 89.3521 bits (220), Expect = 5.104e-21
Identity = 47/105 (44.76%), Postives = 62/105 (59.05%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKR--YQCDKCPRSFSRQSLLRKH 749
            H C  CG++  S   L  H RTH    P +C +CG+AF+ +  L  H R HTGERP+VC +C RAF   SNLRAH + H  ++R  + C +C   F+R   LRKH
Sbjct:  209 HACSECGRRFLSVRELAQHARTHTGERPHVCAECGRAFAAVSTLRKHARTHTGERPHVCPQCGRAFTSSSNLRAHARAHAGQRRPQHACTQCGCRFARAGALRKH 313          

HSP 2 Score: 85.1149 bits (209), Expect = 1.661e-19
Identity = 47/106 (44.34%), Postives = 62/106 (58.49%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH----NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHS 752
            H C LCGK    ++ LK H + H    N P  C +CG+ F  +  L+ H R HTGERP+VC  C RAFA  S LR H +THT  + + C +C R+F+  S LR H+
Sbjct:  179 HVCELCGKAFPHATNLKQHLKRHTEDKNKPHACSECGRRFLSVRELAQHARTHTGERPHVCAECGRAFAAVSTLRKHARTHTGERPHVCPQCGRAFTSSSNLRAHA 284          

HSP 3 Score: 84.3445 bits (207), Expect = 3.361e-19
Identity = 44/103 (42.72%), Postives = 59/103 (57.28%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            H CP CG+     S+L  H RTH    P  C  CG++FS    L  H R+HTGERPY C  C RAF+  ++L+ H   H+  K + C  C ++FS ++ LRKH
Sbjct:    4 HACPQCGRCFALRSSLTKHARTHTGERPYACAACGRSFSVASSLVKHRRVHTGERPYACAACGRAFSQLTSLQKHQGRHSAEKPHACATCGKAFSHRAFLRKH 106          

HSP 4 Score: 65.855 bits (159), Expect = 8.594e-13
Identity = 35/99 (35.35%), Postives = 55/99 (55.56%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTR-------KRYQCDKCPRSF 722
            C  CG+  + +S+L  H+R H    P  C  CG+AFS++  L  H   H+ E+P+ C  C +AF+ R+ LR H +TH  R       +R+ C++C  +F
Sbjct:   34 CAACGRSFSVASSLVKHRRVHTGERPYACAACGRAFSQLTSLQKHQGRHSAEKPHACATCGKAFSHRAFLRKHEKTHAERVAGLVKERRFACERCCATF 132          

HSP 5 Score: 60.077 bits (144), Expect = 8.325e-11
Identity = 33/80 (41.25%), Postives = 45/80 (56.25%), Query Frame = 3
Query:  495 KMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKR--YQCDKCPRSF 722
            + HQ TH    P +C  CGKAFS    L  H   H+G+RP+VC  C +AF   +NL+ H++ HT  K   + C +C R F
Sbjct:  139 QRHQTTHTGERPFVCELCGKAFSHHSTLRKHQSTHSGDRPHVCELCGKAFPHATNLKQHLKRHTEDKNKPHACSECGRRF 218          

HSP 6 Score: 58.5362 bits (140), Expect = 3.217e-10
Identity = 35/110 (31.82%), Postives = 53/110 (48.18%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNPPCI---------CPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            H C  CGK  +  + L+ H++TH              C +C   F        H   HTGERP+VC  C +AF+  S LR H  TH+  + + C+ C ++F   + L++H
Sbjct:   88 HACATCGKAFSHRAFLRKHEKTHAERVAGLVKERRFACERCCATFFDNQSRQRHQTTHTGERPFVCELCGKAFSHHSTLRKHQSTHSGDRPHVCELCGKAFPHATNLKQH 197          
BLAST of Snail-1 vs. Ensembl Sea Lamprey
Match: ZNF436 (zinc finger protein 436 [Source:HGNC Symbol;Acc:HGNC:20814])

HSP 1 Score: 89.3521 bits (220), Expect = 6.112e-21
Identity = 47/105 (44.76%), Postives = 62/105 (59.05%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKR--YQCDKCPRSFSRQSLLRKH 749
            H C  CG++  S   L  H RTH    P +C +CG+AF+ +  L  H R HTGERP+VC +C RAF   SNLRAH + H  ++R  + C +C   F+R   LRKH
Sbjct:  235 HACSECGRRFLSVRELAQHARTHTGERPHVCAECGRAFAAVSTLRKHARTHTGERPHVCPQCGRAFTSSSNLRAHARAHAGQRRPQHACTQCGCRFARAGALRKH 339          

HSP 2 Score: 82.0333 bits (201), Expect = 2.409e-18
Identity = 44/105 (41.90%), Postives = 60/105 (57.14%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKM--HQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            H CP CG+    S  L++  H RTH    P  C  CG++FS    L  H R+HTGERPY C  C RAF+  ++L+ H   H+  K + C  C ++FS ++ LRKH
Sbjct:    1 HACPQCGRCFAESRDLRLTKHARTHTGERPYACAACGRSFSVASSLVKHRRVHTGERPYACAACGRAFSQLTSLQKHQGRHSAEKPHACATCGKAFSHRAFLRKH 105          

HSP 3 Score: 74.7146 bits (182), Expect = 9.394e-16
Identity = 47/133 (35.34%), Postives = 62/133 (46.62%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH-------------------------------NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHS 752
            H C LCGK    ++ LK H + H                               N P  C +CG+ F  +  L+ H R HTGERP+VC  C RAFA  S LR H +THT  + + C +C R+F+  S LR H+
Sbjct:  178 HVCELCGKAFPHATNLKQHLKRHTGERSAAAACCLFSACCNVIKHLRADTAKDKNKPHACSECGRRFLSVRELAQHARTHTGERPHVCAECGRAFAAVSTLRKHARTHTGERPHVCPQCGRAFTSSSNLRAHA 310          

HSP 4 Score: 67.0106 bits (162), Expect = 4.014e-13
Identity = 45/131 (34.35%), Postives = 59/131 (45.04%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGER-----------------------------PYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHS 752
            C LCGK  +  S L+ HQ TH+   P +C  CGKAF     L  HL+ HTGER                             P+ C+ C R F     L  H +THT  + + C +C R+F+  S LRKH+
Sbjct:  152 CELCGKAFSHHSTLRKHQSTHSGDRPHVCELCGKAFPHATNLKQHLKRHTGERSAAAACCLFSACCNVIKHLRADTAKDKNKPHACSECGRRFLSVRELAQHARTHTGERPHVCAECGRAFAAVSTLRKHA 282          

HSP 5 Score: 65.855 bits (159), Expect = 9.825e-13
Identity = 35/99 (35.35%), Postives = 55/99 (55.56%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTR-------KRYQCDKCPRSF 722
            C  CG+  + +S+L  H+R H    P  C  CG+AFS++  L  H   H+ E+P+ C  C +AF+ R+ LR H +TH  R       +R+ C++C  +F
Sbjct:   33 CAACGRSFSVASSLVKHRRVHTGERPYACAACGRAFSQLTSLQKHQGRHSAEKPHACATCGKAFSHRAFLRKHEKTHAERVAGLVKERRFACERCCATF 131          

HSP 6 Score: 58.5362 bits (140), Expect = 3.254e-10
Identity = 35/110 (31.82%), Postives = 53/110 (48.18%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNPPCI---------CPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            H C  CGK  +  + L+ H++TH              C +C   F        H   HTGERP+VC  C +AF+  S LR H  TH+  + + C+ C ++F   + L++H
Sbjct:   87 HACATCGKAFSHRAFLRKHEKTHAERVAGLVKERRFACERCCATFFDNQSRQRHQTTHTGERPFVCELCGKAFSHHSTLRKHQSTHSGDRPHVCELCGKAFPHATNLKQH 196          

HSP 7 Score: 55.4546 bits (132), Expect = 3.517e-9
Identity = 28/65 (43.08%), Postives = 38/65 (58.46%), Query Frame = 3
Query:  501 HQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRK 689
            HQ TH    P +C  CGKAFS    L  H   H+G+RP+VC  C +AF   +NL+ H++ HT  +
Sbjct:  140 HQTTHTGERPFVCELCGKAFSHHSTLRKHQSTHSGDRPHVCELCGKAFPHATNLKQHLKRHTGER 204          
BLAST of Snail-1 vs. Ensembl Yeast
Match: AZF1 (Zinc-finger transcription factor; involved in diauxic shift; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000005639])

HSP 1 Score: 81.2629 bits (199), Expect = 7.039e-18
Identity = 46/133 (34.59%), Postives = 75/133 (56.39%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQC--DKCPRSFSR-------QSLLRKHSLQC-CAKLVDVN*KTPWKNL 809
            HECP C +  + ++ L++H R+H    P +C  CGK F++   L  H R+HTGE+PY C+ C + F+ + NL AH+ TH   K + C  + C ++F++       Q+   K +L    AKL ++N   P +N+
Sbjct:  593 HECPYCHRLFSQATHLEVHVRSHIGYKPFVCDYCGKRFTQGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAHLVTHQKLKPFVCKLENCNKTFTQLGNMKAHQNRFHKETLNALTAKLAEMN---PSENI 722          

HSP 2 Score: 70.4774 bits (171), Expect = 3.166e-14
Identity = 34/87 (39.08%), Postives = 46/87 (52.87%), Query Frame = 3
Query:  489 ALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            A+K H+        CP C + FS+   L  H+R H G +P+VC+ C + F    NLR H + HT  K Y CD C + FSR+  L  H
Sbjct:  589 AVKKHE--------CPYCHRLFSQATHLEVHVRSHIGYKPFVCDYCGKRFTQGGNLRTHERLHTGEKPYSCDICDKKFSRKGNLAAH 667          
BLAST of Snail-1 vs. Ensembl Yeast
Match: CRZ1 (Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium [Source:SGD;Acc:S000004972])

HSP 1 Score: 70.0922 bits (170), Expect = 3.931e-14
Identity = 32/82 (39.02%), Postives = 45/82 (54.88%), Query Frame = 3
Query:  531 CPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCD---------KCPRSFSRQSLLRKH 749
            C  CGK F+R + L  HLR HT ERP++C+ C +AFA + + + H   HT +KRY C           C + F+R   L +H
Sbjct:  571 CDVCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRHEDLHTGKKRYVCGGKLKDGKPWGCGKKFARSDALGRH 652          

HSP 2 Score: 58.5362 bits (140), Expect = 2.708e-10
Identity = 34/90 (37.78%), Postives = 43/90 (47.78%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCN---------RCQRAFADRSNLRAHMQTHTTRK 689
            C +CGKK      LK H RTH    P IC  CGKAF+R      H  +HTG++ YVC           C + FA    L  H +T + R+
Sbjct:  571 CDVCGKKFTRPYNLKSHLRTHTNERPFICSICGKAFARQHDRKRHEDLHTGKKRYVCGGKLKDGKPWGCGKKFARSDALGRHFKTESGRR 660          
BLAST of Snail-1 vs. Ensembl Yeast
Match: ZAP1 (Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains [Source:SGD;Acc:S000003592])

HSP 1 Score: 68.1662 bits (165), Expect = 2.174e-13
Identity = 38/107 (35.51%), Postives = 56/107 (52.34%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQC 761
            ++C  C +  +S   L  H RTH+   P  C  C K F+    L  H+R HTGE+P  C  C + F + SNL  H++TH  +K+Y+C  C +SF     L    ++C
Sbjct:  768 YKCKTCKRCFSSEETLVQHTRTHSGEKPYKCHICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKHIKTH--QKKYKCSDCSKSFDDLGKLNSQKVKC 872          

HSP 2 Score: 67.0106 bits (162), Expect = 5.041e-13
Identity = 35/86 (40.70%), Postives = 48/86 (55.81%), Query Frame = 3
Query:  492 LKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            LK+H +    P  C  C + FS    L  H R H+GE+PY C+ C + FA  S+L+ H++THT  K  QC  C + F+  S L KH
Sbjct:  759 LKVHSKY--KPYKCKTCKRCFSSEETLVQHTRTHSGEKPYKCHICNKKFAISSSLKIHIRTHTGEKPLQCKICGKRFNESSNLSKH 842          

HSP 3 Score: 54.6842 bits (130), Expect = 5.932e-9
Identity = 25/75 (33.33%), Postives = 40/75 (53.33%), Query Frame = 3
Query:  525 CICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C+   C + F +   L  HL++H+  +PY C  C+R F+    L  H +TH+  K Y+C  C + F+  S L+ H
Sbjct:  740 CLWHDCHRTFPQRQKLIRHLKVHSKYKPYKCKTCKRCFSSEETLVQHTRTHSGEKPYKCHICNKKFAISSSLKIH 814          

HSP 4 Score: 46.2098 bits (108), Expect = 2.931e-6
Identity = 28/81 (34.57%), Postives = 40/81 (49.38%), Query Frame = 3
Query:  525 CICPQCGKAFSRMWLLSGHLR-IHT--GERPYVC--NRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHS 752
            C    C K+FS    L+ HL  +H   G+  Y C  + C R F  R  L  H++ H+  K Y+C  C R FS +  L +H+
Sbjct:  707 CQWDGCNKSFSSAQELNDHLEAVHLTRGKSEYQCLWHDCHRTFPQRQKLIRHLKVHSKYKPYKCKTCKRCFSSEETLVQHT 787          
BLAST of Snail-1 vs. Ensembl Yeast
Match: PZF1 (Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA [Source:SGD;Acc:S000006390])

HSP 1 Score: 58.9214 bits (141), Expect = 1.964e-10
Identity = 26/78 (33.33%), Postives = 46/78 (58.97%), Query Frame = 3
Query:  525 CICPQCGKAFSRMWLLSGH-LRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSL 755
            C    C KAF+R  +L+ H L +H G R + C++C ++F  +S+L  H+ TH+  K +QC  C +  + +  L++H +
Sbjct:   51 CDYDGCDKAFTRPSILTEHQLSVHQGLRAFQCDKCAKSFVKKSHLERHLYTHSDTKPFQCSYCGKGVTTRQQLKRHEV 128          
BLAST of Snail-1 vs. Ensembl Yeast
Match: MSN4 (Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes, inducing gene expression; involved in diauxic shift [Source:SGD;Acc:S000001545])

HSP 1 Score: 53.5286 bits (127), Expect = 1.306e-8
Identity = 29/86 (33.72%), Postives = 45/86 (52.33%), Query Frame = 3
Query:  438 SISH-----ECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLR-IHTGERPYVCNRCQRAFADRSNLRAHMQTH 677
            +ISH     + P+  +K  S + +  +    N P  C  C KAF R   L  H+R +H+ ERP+ C  C++ F+   NL  H++TH
Sbjct:  539 TISHNNNNGKVPVQPRKRKSITTIDPNNYDKNKPFKCKDCEKAFRRSEHLKRHIRSVHSTERPFACMFCEKKFSRSDNLSQHLKTH 624          
BLAST of Snail-1 vs. Ensembl Nematostella
Match: SNAILB (Predicted protein; Snail [Source:UniProtKB/TrEMBL;Acc:Q6WGJ9])

HSP 1 Score: 141.354 bits (355), Expect = 1.022e-40
Identity = 66/103 (64.08%), Postives = 76/103 (73.79%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQC 761
            C  C K ++S  ALKMH RTH  PC C  CGKAFSR WLL GH+R HTGE+PY C +CQRAFADRSNLRAH+QTH+  K+Y C +C +SFSR SLL KH   C
Sbjct:  156 CKYCEKLYDSLGALKMHIRTHTLPCKCKICGKAFSRPWLLQGHVRTHTGEKPYKCTQCQRAFADRSNLRAHLQTHSDVKKYSCKQCSKSFSRMSLLLKHEGSC 258          
BLAST of Snail-1 vs. Ensembl Nematostella
Match: EDO40180 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S823])

HSP 1 Score: 117.087 bits (292), Expect = 1.309e-32
Identity = 58/114 (50.88%), Postives = 74/114 (64.91%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            +   ++  C  CGK + S  AL MH  TH     C  CGK+FSR WLL GH R HTGERPY C  C +AFADRSNLRAH+QTH   K+Y+C++C R+F+ +S L KH    C++
Sbjct:   40 LDGKLAKRCHQCGKAYVSMPALAMHVLTHKLLHKCEICGKSFSRPWLLQGHKRSHTGERPYSCPECNKAFADRSNLRAHLQTHNPLKQYKCERCDRTFALKSYLNKHLESACSR 153          
BLAST of Snail-1 vs. Ensembl Nematostella
Match: EDO47708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLK7])

HSP 1 Score: 94.7449 bits (234), Expect = 2.774e-24
Identity = 46/106 (43.40%), Postives = 64/106 (60.38%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQ 758
            H+C +C K  N SS LK H RTH+     +C  CGK F +   L  H+ IHTGE+P+ C +C RAF   SNL+ HM THT    YQC  C + F +++ L++H++ 
Sbjct:   38 HKCEVCNKSFNRSSTLKTHVRTHSEEKAFVCDICGKGFHQKGNLRNHIMIHTGEKPFQCTKCHRAFNKMSNLKFHMHTHTDNLPYQCKTCRKKFEKKTDLKQHNMD 143          

HSP 2 Score: 75.8702 bits (185), Expect = 3.767e-17
Identity = 40/101 (39.60%), Postives = 57/101 (56.44%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQ--RTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C +CGK    SS L  H+   T   P  C  C K+F+R   L  H+R H+ E+ +VC+ C + F  + NLR H+  HT  K +QC KC R+F++ S L+ H
Sbjct:   12 CNICGKGFRLSSTLCRHKIIHTQKKPHKCEVCNKSFNRSSTLKTHVRTHSEEKAFVCDICGKGFHQKGNLRNHIMIHTGEKPFQCTKCHRAFNKMSNLKFH 112          

HSP 3 Score: 60.077 bits (144), Expect = 2.159e-11
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = 3
Query:  498 MHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSL 755
            M   T + P +C  CGK F     L  H  IHT ++P+ C  C ++F   S L+ H++TH+  K + CD C + F ++  LR H +
Sbjct:    1 MPDHTESRPFVCNICGKGFRLSSTLCRHKIIHTQKKPHKCEVCNKSFNRSSTLKTHVRTHSEEKAFVCDICGKGFHQKGNLRNHIM 86          
BLAST of Snail-1 vs. Ensembl Nematostella
Match: EDO40036 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8B6])

HSP 1 Score: 90.5077 bits (223), Expect = 7.958e-22
Identity = 46/101 (45.54%), Postives = 61/101 (60.40%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C  C +     S+L  H+R H    P  C +C KAFS+   L  H+R+HTGE+PY C  C+RAFA   NL+ HM+THT  K Y+CD C + FS+ S L +H
Sbjct:  119 CEFCQRSFTEYSSLAKHRRAHTGERPYKCKECDKAFSQSGGLKVHMRVHTGEKPYPCPLCERAFAKGYNLKTHMRTHTGEKPYKCDFCSKLFSQHSTLMQH 219          

HSP 2 Score: 83.9593 bits (206), Expect = 1.792e-19
Identity = 43/104 (41.35%), Postives = 58/104 (55.77%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
             H+C  CGK+ NS + LK H R H    P  C  C ++F+    L+ H R HTGERPY C  C +AF+    L+ HM+ HT  K Y C  C R+F++   L+ H
Sbjct:   88 EHKCDTCGKQFNSFANLKGHLRIHTGEKPYTCEFCQRSFTEYSSLAKHRRAHTGERPYKCKECDKAFSQSGGLKVHMRVHTGEKPYPCPLCERAFAKGYNLKTH 191          

HSP 3 Score: 80.8777 bits (198), Expect = 2.714e-18
Identity = 42/108 (38.89%), Postives = 59/108 (54.63%), Query Frame = 3
Query:  432 VNSISHECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
               + ++C  C +K      L  H R H P     C  CGK F+    L GHLRIHTGE+PY C  CQR+F + S+L  H + HT  + Y+C +C ++FS+   L+ H
Sbjct:   56 TGDVPYQCHHCPEKLKLRCHLNYHLRIHAPKKEHKCDTCGKQFNSFANLKGHLRIHTGEKPYTCEFCQRSFTEYSSLAKHRRAHTGERPYKCKECDKAFSQSGGLKVH 163          

HSP 4 Score: 66.2402 bits (160), Expect = 5.190e-13
Identity = 32/79 (40.51%), Postives = 46/79 (58.23%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTH 677
            ++C  C K  + S  LK+H R H    P  CP C +AF++ + L  H+R HTGE+PY C+ C + F+  S L  H+Q H
Sbjct:  145 YKCKECDKAFSQSGGLKVHMRVHTGEKPYPCPLCERAFAKGYNLKTHMRTHTGEKPYKCDFCSKLFSQHSTLMQHVQRH 223          

HSP 5 Score: 55.0694 bits (131), Expect = 4.700e-9
Identity = 29/84 (34.52%), Postives = 46/84 (54.76%), Query Frame = 3
Query:  498 MHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            +++ T + P  C  C +       L+ HLRIH  ++ + C+ C + F   +NL+ H++ HT  K Y C+ C RSF+  S L KH
Sbjct:   52 IYRNTGDVPYQCHHCPEKLKLRCHLNYHLRIHAPKKEHKCDTCGKQFNSFANLKGHLRIHTGEKPYTCEFCQRSFTEYSSLAKH 135          

HSP 6 Score: 53.5286 bits (127), Expect = 1.481e-8
Identity = 29/103 (28.16%), Postives = 52/103 (50.49%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTHNPPCI---CPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            +C +C ++  S + L+ H  +H        C  C  AF+  + L  H+  +TG+ PY C+ C      R +L  H++ H  +K ++CD C + F+  + L+ H
Sbjct:    5 QCEVCKEEFTSETKLRKHLSSHKVEVKRLHCDGCKVAFAFQYQLDKHIYRNTGDVPYQCHHCPEKLKLRCHLNYHLRIHAPKKEHKCDTCGKQFNSFANLKGH 107          
BLAST of Snail-1 vs. Ensembl Nematostella
Match: EDO45330 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RT54])

HSP 1 Score: 88.1965 bits (217), Expect = 8.949e-22
Identity = 48/103 (46.60%), Postives = 59/103 (57.28%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            H+C +C K  N SS LK H RTH+     IC  CGK F +   L  H  IHTGE+PY C  C++AF   SNL+ HM  HT    Y+C  C  SFSR+  L+ H
Sbjct:   38 HKCHICNKAFNRSSTLKTHIRTHSEVKEYICEVCGKGFHQKGNLRNHSLIHTGEKPYKCALCEKAFNKLSNLKFHMHVHTDNSPYRCKYCKVSFSRRCDLKSH 140          

HSP 2 Score: 68.1662 bits (165), Expect = 2.489e-14
Identity = 38/101 (37.62%), Postives = 54/101 (53.47%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            C +CGK    SS L  H+  H  + P  C  C KAF+R   L  H+R H+  + Y+C  C + F  + NLR H   HT  K Y+C  C ++F++ S L+ H
Sbjct:   12 CGVCGKGFRLSSTLCRHKIIHTTDRPHKCHICNKAFNRSSTLKTHIRTHSEVKEYICEVCGKGFHQKGNLRNHSLIHTGEKPYKCALCEKAFNKLSNLKFH 112          

HSP 3 Score: 66.2402 bits (160), Expect = 1.405e-13
Identity = 32/78 (41.03%), Postives = 45/78 (57.69%), Query Frame = 3
Query:  522 PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSL 755
            P +C  CGK F     L  H  IHT +RP+ C+ C +AF   S L+ H++TH+  K Y C+ C + F ++  LR HSL
Sbjct:    9 PFVCGVCGKGFRLSSTLCRHKIIHTTDRPHKCHICNKAFNRSSTLKTHIRTHSEVKEYICEVCGKGFHQKGNLRNHSL 86          

HSP 4 Score: 50.0618 bits (118), Expect = 7.322e-8
Identity = 25/74 (33.78%), Postives = 40/74 (54.05%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTH--NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHM 668
            C +CGK  +    L+ H   H    P  C  C KAF+++  L  H+ +HT   PY C  C+ +F+ R +L++H+
Sbjct:   68 CEVCGKGFHQKGNLRNHSLIHTGEKPYKCALCEKAFNKLSNLKFHMHVHTDNSPYRCKYCKVSFSRRCDLKSHI 141          
BLAST of Snail-1 vs. Ensembl Medaka
Match: snai1b (protein snail homolog Sna [Source:NCBI gene;Acc:101160236])

HSP 1 Score: 148.288 bits (373), Expect = 4.818e-43
Identity = 70/107 (65.42%), Postives = 79/107 (73.83%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH--SLQCCA 767
            C  C KK+ S  ALKMH R+H  PC+CP CGKAFSR WLL GH+R HTGERP+ C  C RAFADRSNLRAH+QTH+  K+YQC  C R+FSR SLL KH  S  CCA
Sbjct:  159 CKHCSKKYTSLGALKMHIRSHTLPCVCPTCGKAFSRPWLLRGHIRTHTGERPFACQHCNRAFADRSNLRAHLQTHSEVKKYQCSSCSRTFSRMSLLHKHCASGSCCA 265          
BLAST of Snail-1 vs. Ensembl Medaka
Match: snai1a (protein snail homolog Sna [Source:NCBI gene;Acc:101157208])

HSP 1 Score: 142.124 bits (357), Expect = 9.629e-41
Identity = 68/109 (62.39%), Postives = 79/109 (72.48%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQ-CCAKLV 776
            C  C K++ S  ALKMH R+H  PC+C  CGKAFSR WLL GH+R HTGERP+ C  C RAFADRSNLRAH+QTH   K+YQC  C R+FSR SLL+KHS   CC+  V
Sbjct:  148 CKHCPKEYTSLGALKMHIRSHTLPCVCTTCGKAFSRPWLLRGHIRTHTGERPFSCPHCNRAFADRSNLRAHLQTHAEVKKYQCGVCSRTFSRMSLLQKHSSSGCCSTSV 256          
BLAST of Snail-1 vs. Ensembl Medaka
Match: snai2 (snail family transcriptional repressor 2 [Source:NCBI gene;Acc:101173257])

HSP 1 Score: 139.813 bits (351), Expect = 7.452e-40
Identity = 65/107 (60.75%), Postives = 76/107 (71.03%), Query Frame = 3
Query:  444 SHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            S  C  C K++ S  ALKMH RTH  PC+C  CGKAFSR WLL GH+R HTGE+P+ C  C RAFADRSNLRAH+QTH+  K+YQC  C ++FSR SLL KH    C
Sbjct:  150 SFSCKYCEKEYVSLGALKMHIRTHTLPCVCKICGKAFSRPWLLQGHIRTHTGEKPFSCPHCNRAFADRSNLRAHLQTHSDVKKYQCKNCSKTFSRMSLLHKHEESGC 256          
BLAST of Snail-1 vs. Ensembl Medaka
Match: scrt1a (transcriptional repressor scratch 1 [Source:NCBI gene;Acc:101155400])

HSP 1 Score: 125.176 bits (313), Expect = 9.548e-34
Identity = 58/110 (52.73%), Postives = 75/110 (68.18%), Query Frame = 3
Query:  441 ISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            ++ +CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K Y+C +C ++F+ +S L KH    C K
Sbjct:  181 MAKKCPTCGKIYVSMPAMAMHLLTHDLKHKCDVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHYKCKRCNKTFALKSYLNKHYESACFK 290          
BLAST of Snail-1 vs. Ensembl Medaka
Match: ENSORLT00000047079.1 (scratch family transcriptional repressor 1 [Source:NCBI gene;Acc:101167481])

HSP 1 Score: 124.405 bits (311), Expect = 1.075e-33
Identity = 57/114 (50.00%), Postives = 77/114 (67.54%), Query Frame = 3
Query:  429 IVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAK 770
            + + ++ +CP CGK + S  A+ MH  TH+    C  CGKAFSR WLL GH+R HTGE+P+ C  C +AFADRSNLRAHMQTH+  K ++C +C ++F+ +S L KH    C K
Sbjct:  156 LDSKLAKKCPTCGKVYVSMPAMAMHLLTHDLKHKCDVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHFKCKRCNKTFALKSYLNKHYESACFK 269          
BLAST of Snail-1 vs. Planmine SMEST
Match: SMESG000076673.1 (SMESG000076673.1)

HSP 1 Score: 457.603 bits (1176), Expect = 2.652e-165
Identity = 234/236 (99.15%), Postives = 235/236 (99.58%), Query Frame = 3
Query:   78 MVPNVAKFNVIQMIVSSIRNQMTNSTCKPIDIMNNNQKFEIIRRATYLYHYFVSQQERVPFRQPLYPAWNLKLINYPSPSTFPSLLNSENPNFLTTESIADTXXXXXXXXXXXXXIFIVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN 785
            MVPNVAKFNVIQMIVSSIRNQMTNSTCKPIDIMNNNQKFEIIRRATYLY+YFVSQQERVPFRQPLYPAWNLKLINYPSPSTFPSLLNSENPNFL TESIADTDSGLPSSDSSGKSIFIVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN
Sbjct:    1 MVPNVAKFNVIQMIVSSIRNQMTNSTCKPIDIMNNNQKFEIIRRATYLYYYFVSQQERVPFRQPLYPAWNLKLINYPSPSTFPSLLNSENPNFLPTESIADTDSGLPSSDSSGKSIFIVNSISHECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN 236          
BLAST of Snail-1 vs. Planmine SMEST
Match: SMESG000064846.1 (SMESG000064846.1)

HSP 1 Score: 147.517 bits (371), Expect = 1.890e-42
Identity = 68/105 (64.76%), Postives = 78/105 (74.29%), Query Frame = 3
Query:  447 HECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQC 761
            + C  C K + S SALKMH RTH  PC C  CGKAFSRMWLL+GH+R HTGE+P+ C+ C RAFADRSNLRAHMQTH+  KRYQCD C ++FSR  LL KH   C
Sbjct:  198 YSCRQCNKVYFSLSALKMHVRTHTLPCKCTICGKAFSRMWLLTGHMRTHTGEKPFSCSECDRAFADRSNLRAHMQTHSQVKRYQCDLCHKTFSRMGLLTKHKSSC 302          
BLAST of Snail-1 vs. Planmine SMEST
Match: SMESG000041786.1 (SMESG000041786.1)

HSP 1 Score: 124.02 bits (310), Expect = 5.763e-33
Identity = 60/105 (57.14%), Postives = 71/105 (67.62%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCC 764
            +CP C K + S  AL MH  TH+    C  CGKAFSR WLL GH R HTGE+PY C  C RAFADRSNLRAHMQTH++ K Y+C+ C R+F+ +S L KH    C
Sbjct:  263 KCPHCDKIYVSMPALSMHILTHDLRHQCDICGKAFSRPWLLQGHRRAHTGEKPYGCAHCGRAFADRSNLRAHMQTHSSMKTYECNACKRNFALKSYLNKHLESSC 367          
BLAST of Snail-1 vs. Planmine SMEST
Match: SMESG000067393.1 (SMESG000067393.1)

HSP 1 Score: 108.997 bits (271), Expect = 1.185e-28
Identity = 49/86 (56.98%), Postives = 61/86 (70.93%), Query Frame = 3
Query:  522 PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVD 779
            P +C  CGK F+R WLL GH+R HTGERP+ CN C + FAD+SNLRAH+QTH+T K +QC KC R FS +S L KH    C K ++
Sbjct:  119 PSLCNFCGKTFTRPWLLQGHVRTHTGERPFKCNICGKTFADKSNLRAHIQTHSTVKPFQCSKCCRCFSLKSYLAKHKASSCDKKLN 204          
BLAST of Snail-1 vs. Planmine SMEST
Match: SMESG000000679.1 (SMESG000000679.1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.975e-20
Identity = 43/103 (41.75%), Postives = 65/103 (63.11%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHS 752
            +C LCG+ +   S LK+H RTH+   P  C  C K FS++  L+ H RIH+GE+P+ C+ C R F+  S++  HM+TH+  + Y+C  C ++FS  S L KH+
Sbjct:  318 QCHLCGRSYARPSTLKIHMRTHSGERPFKCNVCSKTFSQVANLTAHKRIHSGEKPFSCHICCRTFSQSSSVTTHMRTHSGERPYRCHHCSKAFSDSSTLTKHT 420          

HSP 2 Score: 75.0998 bits (183), Expect = 4.574e-15
Identity = 37/102 (36.27%), Postives = 57/102 (55.88%), Query Frame = 3
Query:  450 ECPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            +C +C K  +  + L  H+R H+   P  C  C + FS+   ++ H+R H+GERPY C+ C +AF+D S L  H + H+  K Y+C  C   FS+   L +H
Sbjct:  346 KCNVCSKTFSQVANLTAHKRIHSGEKPFSCHICCRTFSQSSSVTTHMRTHSGERPYRCHHCSKAFSDSSTLTKHTRVHSGEKPYKCRICLLKFSQSGNLNRH 447          

HSP 3 Score: 68.1662 bits (165), Expect = 7.541e-13
Identity = 30/78 (38.46%), Postives = 47/78 (60.26%), Query Frame = 3
Query:  516 NPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKH 749
            + P  C  CG++++R   L  H+R H+GERP+ CN C + F+  +NL AH + H+  K + C  C R+FS+ S +  H
Sbjct:  314 DEPNQCHLCGRSYARPSTLKIHMRTHSGERPFKCNVCSKTFSQVANLTAHKRIHSGEKPFSCHICCRTFSQSSSVTTH 391          

HSP 4 Score: 62.3882 bits (150), Expect = 7.855e-11
Identity = 31/77 (40.26%), Postives = 43/77 (55.84%), Query Frame = 3
Query:  453 CPLCGKKHNSSSALKMHQRTHNP--PCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAFADRSNLRAHMQTH 677
            C +C +  + SS++  H RTH+   P  C  C KAFS    L+ H R+H+GE+PY C  C   F+   NL  HM+ H
Sbjct:  375 CHICCRTFSQSSSVTTHMRTHSGERPYRCHHCSKAFSDSSTLTKHTRVHSGEKPYKCRICLLKFSQSGNLNRHMRVH 451          
The following BLAST results are available for this feature:
BLAST of Snail-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SNAI29.899e-4060.75snail family transcriptional repressor 2 [Source:H... [more]
SNAI11.072e-3960.95snail family transcriptional repressor 1 [Source:H... [more]
SNAI32.264e-3860.58snail family transcriptional repressor 3 [Source:H... [more]
SCRT11.459e-3351.75scratch family transcriptional repressor 1 [Source... [more]
SCRT11.459e-3351.75scratch family transcriptional repressor 1 [Source... [more]
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BLAST of Snail-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
snai-12.393e-3753.64SNAIl family zinc finger 2 transcription factor ho... [more]
ces-12.065e-3254.81CES-1; CEll death Specification [Source:UniProtKB... [more]
scrt-11.204e-2652.22SCRaTch (Zinc finger transcriptional repressor) ho... [more]
che-13.587e-1941.58Transcription factor che-1 [Source:UniProtKB/Swis... [more]
che-16.535e-1941.58Transcription factor che-1 [Source:UniProtKB/Swis... [more]
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BLAST of Snail-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sna9.023e-3961.68gene:FBgn0003448 transcript:FBtr0080739[more]
wor8.763e-3863.46gene:FBgn0001983 transcript:FBtr0080740[more]
esg3.281e-3662.14gene:FBgn0001981 transcript:FBtr0080734[more]
Kah1.417e-3250.00gene:FBgn0035144 transcript:FBtr0072563[more]
scrt8.833e-3154.21gene:FBgn0004880 transcript:FBtr0333136[more]
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BLAST of Snail-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
snai1a3.697e-4363.06snail family zinc finger 1a [Source:ZFIN;Acc:ZDB-G... [more]
snai1a1.894e-4267.00snail family zinc finger 1a [Source:ZFIN;Acc:ZDB-G... [more]
snai1b2.090e-4163.89snail family zinc finger 1b [Source:ZFIN;Acc:ZDB-G... [more]
snai22.909e-4060.75snail family zinc finger 2 [Source:ZFIN;Acc:ZDB-GE... [more]
snai34.768e-3859.81snail family zinc finger 3 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Snail-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
snai11.825e-3960.75snail family zinc finger 1 [Source:NCBI gene;Acc:3... [more]
srpk22.477e-3960.75SRSF protein kinase 2 [Source:Xenbase;Acc:XB-GENE-... [more]
SNAI32.370e-3761.54snail family zinc finger 3 [Source:NCBI gene;Acc:1... [more]
SCRT11.591e-3352.34scratch family transcriptional repressor 1 [Source... [more]
ENSXETT00000046689.14.106e-2346.60zinc finger protein 84 [Source:NCBI gene;Acc:49679... [more]
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BLAST of Snail-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Snai11.335e-4061.90snail family zinc finger 1 [Source:MGI Symbol;Acc:... [more]
Snai28.534e-4060.75snail family zinc finger 2 [Source:MGI Symbol;Acc:... [more]
Snai31.032e-3962.50snail family zinc finger 3 [Source:MGI Symbol;Acc:... [more]
Scrt28.669e-3552.14scratch family zinc finger 2 [Source:MGI Symbol;Ac... [more]
Scrt19.505e-3449.59scratch family zinc finger 1 [Source:MGI Symbol;Ac... [more]
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BLAST of Snail-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P19382|SNAI1_XENLA8.940e-4060.75Protein snail homolog Sna OS=Xenopus laevis OX=835... [more]
sp|Q02085|SNAI1_MOUSE9.339e-4061.90Zinc finger protein SNAI1 OS=Mus musculus OX=10090... [more]
sp|Q3MHQ4|SNAI2_BOVIN1.308e-3961.47Zinc finger protein SNAI2 OS=Bos taurus OX=9913 GN... [more]
sp|O43623|SNAI2_HUMAN4.754e-3960.75Zinc finger protein SNAI2 OS=Homo sapiens OX=9606 ... [more]
sp|O95863|SNAI1_HUMAN5.148e-3960.95Zinc finger protein SNAI1 OS=Homo sapiens OX=9606 ... [more]
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BLAST of Snail-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A221SAL39.096e-175100.00Snail-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV... [more]
A0A504YF643.403e-4165.18Protein escargot OS=Fasciola gigantica OX=46835 GN... [more]
A0A183B5V04.044e-4164.86Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A4E0RCV14.649e-4165.18Protein escargot OS=Fasciola hepatica OX=6192 GN=D... [more]
A0A183QHE81.481e-4066.98Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
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BLAST of Snail-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
snai1b1.501e-4162.04protein snail homolog Sna-like [Source:NCBI gene;A... [more]
snai22.827e-4060.75snail family transcriptional repressor 2 [Source:N... [more]
ENSAMXT00000011311.22.268e-3859.81pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
SCRT13.272e-3450.00transcriptional repressor scratch 1-like [Source:N... [more]
scrt1a5.052e-3452.73transcriptional repressor scratch 1-like [Source:N... [more]
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BLAST of Snail-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000007831.12.452e-2646.00pep scaffold:Pmarinus_7.0:GL477655:47469:47963:1 g... [more]
ENSPMAT00000000467.11.120e-2145.28pep scaffold:Pmarinus_7.0:GL480927:176958:178556:1... [more]
zgc:1130304.063e-2147.52zgc:113030 [Source:ZFIN;Acc:ZDB-GENE-050522-528][more]
ZNF4365.104e-2144.76zinc finger protein 436 [Source:HGNC Symbol;Acc:HG... [more]
ZNF4366.112e-2144.76zinc finger protein 436 [Source:HGNC Symbol;Acc:HG... [more]
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BLAST of Snail-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
AZF17.039e-1834.59Zinc-finger transcription factor; involved in diau... [more]
CRZ13.931e-1439.02Transcription factor, activates transcription of s... [more]
ZAP12.174e-1335.51Zinc-regulated transcription factor; binds to zinc... [more]
PZF11.964e-1033.33Transcription factor IIIA (TFIIIA); essential DNA ... [more]
MSN41.306e-833.72Stress-responsive transcriptional activator; activ... [more]
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BLAST of Snail-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
SNAILB1.022e-4064.08Predicted protein; Snail [Source:UniProtKB/TrEMBL... [more]
EDO401801.309e-3250.88Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO477082.774e-2443.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO400367.958e-2245.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO453308.949e-2246.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Snail-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
snai1b4.818e-4365.42protein snail homolog Sna [Source:NCBI gene;Acc:10... [more]
snai1a9.629e-4162.39protein snail homolog Sna [Source:NCBI gene;Acc:10... [more]
snai27.452e-4060.75snail family transcriptional repressor 2 [Source:N... [more]
scrt1a9.548e-3452.73transcriptional repressor scratch 1 [Source:NCBI g... [more]
ENSORLT00000047079.11.075e-3350.00scratch family transcriptional repressor 1 [Source... [more]
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BLAST of Snail-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000076673.12.652e-16599.15SMESG000076673.1[more]
SMESG000064846.11.890e-4264.76SMESG000064846.1[more]
SMESG000041786.15.763e-3357.14SMESG000041786.1[more]
SMESG000067393.11.185e-2856.98SMESG000067393.1[more]
SMESG000000679.11.975e-2041.75SMESG000000679.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004536 ID=SMED30004536|Name=Snail-1|organism=Schmidtea mediterranea sexual|type=transcript|length=824bp
TAGCAATCAATCCTAAAGTCGAATAAATTTTCAAAATTATGGAATCTACT
TTCGAAAATTATGAGTTAGTTCATCAAATGGTACCGAATGTGGCCAAGTT
CAATGTTATTCAGATGATTGTCAGCTCGATTAGAAATCAAATGACAAATT
CGACTTGTAAACCGATAGATATCATGAATAATAATCAAAAATTTGAAATC
ATTCGCAGAGCAACATATCTTTATCATTATTTTGTATCACAACAAGAACG
AGTACCTTTTCGCCAGCCGTTGTATCCGGCCTGGAATCTGAAATTAATTA
ACTATCCGTCTCCTAGCACGTTCCCGTCGTTGTTAAATTCTGAAAATCCA
AACTTTCTGACAACAGAATCCATTGCTGATACAGATAGTGGTCTTCCAAG
CTCCGACAGTTCTGGGAAATCAATATTCATAGTGAATTCAATATCTCACG
AATGTCCCTTGTGCGGTAAGAAACACAACAGTTCAAGTGCTTTGAAAATG
CATCAGCGGACCCATAATCCGCCATGTATTTGCCCTCAATGTGGCAAAGC
GTTCTCTCGAATGTGGTTACTTTCGGGTCATCTGAGAATCCATACTGGTG
AAAGGCCATATGTTTGCAATAGATGTCAGAGAGCGTTTGCAGACCGATCT
AATTTACGAGCTCATATGCAAACGCATACGACTCGTAAACGTTACCAGTG
TGATAAATGTCCCCGTTCATTTAGCCGACAGTCCTTGTTGCGCAAGCATT
CATTACAGTGCTGTGCAAAACTTGTCGATGTTAACTAAAAAACTCCGTGG
AAGAATCTATCGAATTGTCGAATA
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protein sequence of SMED30004536-orf-1

>SMED30004536-orf-1 ID=SMED30004536-orf-1|Name=SMED30004536-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
MESTFENYELVHQMVPNVAKFNVIQMIVSSIRNQMTNSTCKPIDIMNNNQ
KFEIIRRATYLYHYFVSQQERVPFRQPLYPAWNLKLINYPSPSTFPSLLN
SENPNFLTTESIADTDSGLPSSDSSGKSIFIVNSISHECPLCGKKHNSSS
ALKMHQRTHNPPCICPQCGKAFSRMWLLSGHLRIHTGERPYVCNRCQRAF
ADRSNLRAHMQTHTTRKRYQCDKCPRSFSRQSLLRKHSLQCCAKLVDVN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000136whole organism
PLANA:0000419prepharyngeal region
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR036236Znf_C2H2_sf
IPR015880Znf_C2H2-like
IPR007087Znf_C2H2
IPR013087Znf_C2H2_type
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
GO:0003676nucleic acid binding
GO:0003677DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013087Zinc finger C2H2-typeSMARTSM00355c2h2final6coord: 219..239
e-value: 16.0
score: 11.4
coord: 191..213
e-value: 3.9E-4
score: 29.8
coord: 137..159
e-value: 0.006
score: 25.8
coord: 163..185
e-value: 0.048
score: 22.8
IPR013087Zinc finger C2H2-typePFAMPF00096zf-C2H2coord: 219..237
e-value: 1.9E-5
score: 24.8
coord: 164..185
e-value: 0.0021
score: 18.4
coord: 191..213
e-value: 2.8E-6
score: 27.5
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 163..185
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 193..213
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 137..159
score: 10.637
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 163..190
score: 14.856
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 219..237
score: 9.723
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 191..218
score: 13.63
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 213..240
e-value: 5.3E-7
score: 31.5
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 136..159
e-value: 1.6E-5
score: 26.6
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 160..187
e-value: 2.1E-11
score: 45.4
NoneNo IPR availablePFAMPF12874zf-metcoord: 138..157
e-value: 0.0039
score: 17.5
NoneNo IPR availableGENE3DG3DSA:3.30.160.60coord: 188..212
e-value: 6.2E-14
score: 53.5
NoneNo IPR availablePANTHERPTHR24377FAMILY NOT NAMEDcoord: 137..238
NoneNo IPR availablePANTHERPTHR24377:SF772IP01015P-RELATEDcoord: 137..238
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 136..174
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 204..238
IPR036236Zinc finger C2H2 superfamilySUPERFAMILYSSF57667beta-beta-alpha zinc fingerscoord: 162..213