signal peptidase complex subunit 1

Overview
Namesignal peptidase complex subunit 1
Smed IDSMED30004516
Length (bp)464
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of signal peptidase complex subunit 1 (SMED30004516) t-SNE clustered cells

Violin plots show distribution of expression levels for signal peptidase complex subunit 1 (SMED30004516) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of signal peptidase complex subunit 1 (SMED30004516) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for signal peptidase complex subunit 1 (SMED30004516) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004516

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30004516SMESG000027186.1 dd_Smed_v4_992_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004516SMESG000027186.1 dd_Smed_v4_992_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004516SMESG000027186.1 dd_Smed_v4_992_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004516SMESG000027186.1 dd_Smed_v4_992_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004516SMESG000027186.1 Contig44261newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004516SMESG000027186.1 Contig44261uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Human
Match: SPCS1 (signal peptidase complex subunit 1 [Source:HGNC Symbol;Acc:HGNC:23401])

HSP 1 Score: 56.9954 bits (136), Expect = 8.303e-11
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQE 352
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V E+ T  +
Sbjct:    3 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQESSTDDK 88
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Human
Match: SPCS1 (signal peptidase complex subunit 1 [Source:HGNC Symbol;Acc:HGNC:23401])

HSP 1 Score: 56.6102 bits (135), Expect = 4.231e-10
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQE 352
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V E+ T  +
Sbjct:   70 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQESSTDDK 155
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Human
Match: SPCS1 (signal peptidase complex subunit 1 [Source:HGNC Symbol;Acc:HGNC:23401])

HSP 1 Score: 45.0542 bits (105), Expect = 1.568e-6
Identity = 18/51 (35.29%), Postives = 28/51 (54.90%), Query Frame = 2 
Query:  200 WGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQE 352
            +GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V E+ T  +
Sbjct:   16 YGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQESSTDDK 66
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Celegans
Match: spcs-1 (Probable signal peptidase complex subunit 1 [Source:UniProtKB/Swiss-Prot;Acc:O44953])

HSP 1 Score: 46.595 bits (109), Expect = 3.213e-7
Identity = 33/88 (37.50%), Postives = 53/88 (60.23%), Query Frame = 2 
Query:  101 IESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPF-YRKNSLNWQKVLENETQQETGD 361
            ++ +  H+DF GQK AE+ +Q I+ I GIIGF+ G++ QQ+S  +  +      +  +++ PWPF +RKN + W    E    QE+GD
Sbjct:   12 LQKLSSHIDFQGQKVAERTYQVILTIAGIIGFLVGFWTQQLSYAMFTVLGASAFTALIILPPWPFLFRKNPIVWHTPAEP---QESGD 96
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Fly
Match: Spase12 (gene:FBgn0040623 transcript:FBtr0085435)

HSP 1 Score: 68.9366 bits (167), Expect = 8.546e-16
Identity = 35/80 (43.75%), Postives = 52/80 (65.00%), Query Frame = 2 
Query:  107 SIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQ 346
             I  H+DF GQ  AE+  + II   GI+G ++G + QQ SQT+ +L  GFVLS  + + PWP YR+N+L WQK ++ + +
Sbjct:    3 DIQTHMDFAGQGKAERWSRFIITFFGIVGLVYGAFVQQFSQTVYILGAGFVLSSLITIPPWPLYRRNALKWQKPIDTDAK 82
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Zebrafish
Match: spcs1 (signal peptidase complex subunit 1 [Source:ZFIN;Acc:ZDB-GENE-050809-130])

HSP 1 Score: 63.5438 bits (153), Expect = 1.356e-13
Identity = 42/86 (48.84%), Postives = 57/86 (66.28%), Query Frame = 2 
Query:  104 ESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQETGD 361
            +SIP H+D+ GQK AE+IFQ II+++  IGFI+G   QQ   T+ ++  GF +S  L + PWP YRK+ LNWQ  L  ET  ET +
Sbjct:    6 KSIPTHMDYKGQKLAEQIFQGIILVSAAIGFIYGLIVQQFGWTVYIMLAGFTVSCLLTLPPWPMYRKSPLNWQPALP-ETPAETRE 90
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Xenopus
Match: mrpl44 (mitochondrial ribosomal protein L44 [Source:Xenbase;Acc:XB-GENE-1000272])

HSP 1 Score: 48.9062 bits (115), Expect = 1.236e-7
Identity = 31/72 (43.06%), Postives = 47/72 (65.28%), Query Frame = 2 
Query:  104 ESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNW 319
             S+   LDF GQK AE+IFQ II+ + ++GF++GY  +Q   T+ ++  GF +S  L + PWP YR++ L W
Sbjct:   20 ASLSLSLDFKGQKLAEQIFQGIILFSAVLGFLYGYVIEQFGWTVYIVIAGFAVSCLLTLPPWPMYRRHPLKW 91
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Mouse
Match: Spcs1 (signal peptidase complex subunit 1 homolog (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:1916269])

HSP 1 Score: 55.8398 bits (133), Expect = 1.577e-10
Identity = 35/90 (38.89%), Postives = 58/90 (64.44%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQ-QETGD 361
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V +  T+ +++GD
Sbjct:    3 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQDLGTEDKKSGD 92
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Mouse
Match: Spcs1 (signal peptidase complex subunit 1 homolog (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:1916269])

HSP 1 Score: 55.4546 bits (132), Expect = 7.277e-10
Identity = 35/90 (38.89%), Postives = 58/90 (64.44%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQ-QETGD 361
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V +  T+ +++GD
Sbjct:   62 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQDLGTEDKKSGD 151
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Mouse
Match: Spcs1 (signal peptidase complex subunit 1 homolog (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:1916269])

HSP 1 Score: 55.4546 bits (132), Expect = 7.277e-10
Identity = 35/90 (38.89%), Postives = 58/90 (64.44%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQ-QETGD 361
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V +  T+ +++GD
Sbjct:   62 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQDLGTEDKKSGD 151
[Close]
BLAST of signal peptidase complex subunit 1 vs. UniProt/SwissProt
Match: sp|Q9VAL0|SPCS1_DROME (Signal peptidase complex subunit 1 OS=Drosophila melanogaster OX=7227 GN=Spase12 PE=1 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 8.566e-15
Identity = 35/80 (43.75%), Postives = 52/80 (65.00%), Query Frame = 2 
Query:  107 SIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQ 346
             I  H+DF GQ  AE+  + II   GI+G ++G + QQ SQT+ +L  GFVLS  + + PWP YR+N+L WQK ++ + +
Sbjct:    3 DIQTHMDFAGQGKAERWSRFIITFFGIVGLVYGAFVQQFSQTVYILGAGFVLSSLITIPPWPLYRRNALKWQKPIDTDAK 82
[Close]
BLAST of signal peptidase complex subunit 1 vs. UniProt/SwissProt
Match: sp|Q3T134|SPCS1_BOVIN (Signal peptidase complex subunit 1 OS=Bos taurus OX=9913 GN=SPCS1 PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 5.481e-10
Identity = 33/86 (38.37%), Postives = 55/86 (63.95%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQE 352
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V ++ T+ +
Sbjct:    3 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYLAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQDSSTEDK 88
[Close]
BLAST of signal peptidase complex subunit 1 vs. UniProt/SwissProt
Match: sp|B0FWK4|SPCS1_PIG (Signal peptidase complex subunit 1 OS=Sus scrofa OX=9823 GN=SPCS1 PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.035e-9
Identity = 33/86 (38.37%), Postives = 55/86 (63.95%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQE 352
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V ++ T+ +
Sbjct:    3 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQDSGTEDK 88
[Close]
BLAST of signal peptidase complex subunit 1 vs. UniProt/SwissProt
Match: sp|Q5RF96|SPCS1_PONAB (Signal peptidase complex subunit 1 OS=Pongo abelii OX=9601 GN=SPCS1 PE=2 SV=2)

HSP 1 Score: 56.6102 bits (135), Expect = 1.740e-9
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQE 352
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V E+ T  +
Sbjct:   70 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQESSTDDK 155
[Close]
BLAST of signal peptidase complex subunit 1 vs. UniProt/SwissProt
Match: sp|Q9Y6A9|SPCS1_HUMAN (Signal peptidase complex subunit 1 OS=Homo sapiens OX=9606 GN=SPCS1 PE=1 SV=5)

HSP 1 Score: 56.6102 bits (135), Expect = 2.032e-9
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQE 352
            + + S+P  +D+ GQK AE++FQ II+ + I+GFI+GY  +Q   T+ ++  GF  S  L + PWP YR++ L W  V E+ T  +
Sbjct:   70 EHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLLTLPPWPIYRRHPLKWLPVQESSTDDK 155
[Close]
BLAST of signal peptidase complex subunit 1 vs. TrEMBL
Match: A0A267GKY1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009815g1 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.318e-17
Identity = 44/93 (47.31%), Postives = 57/93 (61.29%), Query Frame = 2 
Query:   89 IQDRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENET--QQETGD 361
            + D    IP H+DF GQ+ AE+ FQ ++ I G+ G  WGY CQQ SQT+L+LA GF LS  L + PWP YR++ L WQ V       + ETG 
Sbjct:    4 LTDLTSRIPTHMDFEGQRRAERTFQFVLAIFGLAGLAWGYICQQFSQTVLILAAGFALSALLTLPPWPMYRRSQLRWQPVRPAAATGEAETGS 96
[Close]
BLAST of signal peptidase complex subunit 1 vs. TrEMBL
Match: A0A267F4N6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017032g2 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.406e-17
Identity = 44/93 (47.31%), Postives = 58/93 (62.37%), Query Frame = 2 
Query:   89 IQDRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENET--QQETGD 361
            + D    IP H+DF GQ+ AE+ FQ ++ I G+ G  WGY CQQ SQT+L+LA GF LS  L + PWP YR++ L+WQ V       + ETG 
Sbjct:    4 LTDLTSRIPTHMDFEGQRRAERTFQFVLAIFGVAGLAWGYICQQFSQTVLILAAGFALSALLTLPPWPMYRRSQLHWQPVRPAAATGEAETGS 96
[Close]
BLAST of signal peptidase complex subunit 1 vs. TrEMBL
Match: A0A2H1V8V7 (SFRICE_004166 OS=Spodoptera frugiperda OX=7108 GN=SFRICE_004166 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 5.305e-17
Identity = 41/84 (48.81%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQ 346
            D   SIP H+D++GQ  AEK+++ II +  I+GF+WGY  QQ SQ++ +L  GF+L+  L V PWP YR+N LNWQ    NE +
Sbjct:    2 DFFTSIPTHIDYVGQAKAEKLYRAIITLFSIVGFVWGYIVQQFSQSVYILGAGFILAAILTVPPWPMYRRNPLNWQNPRSNEEK 85
[Close]
BLAST of signal peptidase complex subunit 1 vs. TrEMBL
Match: A0A5N4A7E3 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_12853 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 5.785e-17
Identity = 42/87 (48.28%), Postives = 56/87 (64.37%), Query Frame = 2 
Query:   95 DRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQET 355
            D I SIP H+DF GQ  AEK  + II + G +G IWGY  QQ SQT+ +LA GF+L+  L + PWP YR+  L WQKV   ++ + +
Sbjct:    2 DFINSIPTHMDFEGQSRAEKYSRVIITLFGAVGVIWGYIIQQFSQTVYILAAGFLLAAILTIPPWPIYRRRPLQWQKVRSGDSGEPS 88
[Close]
BLAST of signal peptidase complex subunit 1 vs. TrEMBL
Match: A0A1D2AIC2 (Signal peptidase complex subunit 1 (Fragment) OS=Ornithodoros brasiliensis OX=888526 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 9.555e-17
Identity = 43/79 (54.43%), Postives = 57/79 (72.15%), Query Frame = 2 
Query:  101 IESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLEN 337
            ++S+P H+DF GQ+ AEKIFQ + V+  +IG  WGY  QQ S T+++L VGFVLS  L + PWPFYR+ SL WQKV + 
Sbjct:   18 LKSLPTHMDFEGQRRAEKIFQFVTVLFAVIGLFWGYAVQQFSYTVIILGVGFVLSCLLTLPPWPFYRRKSLAWQKVRDE 96
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Cavefish
Match: spcs1 (signal peptidase complex subunit 1 [Source:NCBI gene;Acc:103040433])

HSP 1 Score: 57.7658 bits (138), Expect = 2.129e-11
Identity = 36/76 (47.37%), Postives = 52/76 (68.42%), Query Frame = 2 
Query:  104 ESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVL 331
            +SIP H+D+ GQK AE+IFQ II+++ +IGFI+G   +Q   T+ ++  GF +S  L + PWP YR+N L WQ  L
Sbjct:    6 KSIPTHMDYKGQKLAEQIFQGIILVSAVIGFIYGLIIEQFGWTVYIMLAGFTVSCLLTLPPWPMYRQNPLTWQPAL 81
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Nematostella
Match: EDO38482 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCR5])

HSP 1 Score: 70.0922 bits (170), Expect = 1.521e-16
Identity = 40/84 (47.62%), Postives = 56/84 (66.67%), Query Frame = 2 
Query:  104 ESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQET 355
            +SIP HLD+ GQK AEK+F  II+  G+ GF+WGYY +Q   T+++L  GFV+S  +V+ PWP +RK+ L WQK    +   E 
Sbjct:    7 KSIPTHLDYEGQKLAEKLFHIIIIFFGVAGFLWGYYVEQFGATVMILIAGFVVSCLVVLPPWPCFRKHPLQWQKPRPEKQNPEA 90
[Close]
BLAST of signal peptidase complex subunit 1 vs. Ensembl Medaka
Match: spcs1 (signal peptidase complex subunit 1 [Source:NCBI gene;Acc:101160314])

HSP 1 Score: 58.9214 bits (141), Expect = 7.976e-12
Identity = 40/84 (47.62%), Postives = 57/84 (67.86%), Query Frame = 2 
Query:  104 ESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQET 355
            +SIP H+D+ GQK AE+IFQ II+I+ +IGF++G   +Q   T+ ++  GF +S  L + PWP YRKN L WQ V+  ET  E+
Sbjct:    6 KSIPTHMDYKGQKLAEQIFQGIILISAVIGFVYGLIVEQFGWTVYIVLGGFAVSCLLTLPPWPMYRKNPLPWQPVVP-ETSAES 88
[Close]
BLAST of signal peptidase complex subunit 1 vs. Planmine SMEST
Match: SMESG000027186.1 (SMESG000027186.1)

HSP 1 Score: 169.474 bits (428), Expect = 4.651e-55
Identity = 98/98 (100.00%), Postives = 98/98 (100.00%), Query Frame = 2 
Query:   71 MNYFQNIQDRIESIPHHLDFIGQKHAEKXXXXXXXXXXXXXXXWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQETGDV 364
            MNYFQNIQDRIESIPHHLDFIGQKHAEKIFQCIIVITGIIGFIWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQETGDV
Sbjct:    1 MNYFQNIQDRIESIPHHLDFIGQKHAEKIFQCIIVITGIIGFIWGYYCQQISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQETGDV 98
The following BLAST results are available for this feature:
BLAST of signal peptidase complex subunit 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SPCS18.303e-1139.53signal peptidase complex subunit 1 [Source:HGNC Sy... [more]
SPCS14.231e-1039.53signal peptidase complex subunit 1 [Source:HGNC Sy... [more]
SPCS11.568e-635.29signal peptidase complex subunit 1 [Source:HGNC Sy... [more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spcs-13.213e-737.50Probable signal peptidase complex subunit 1 [Sour... [more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Spase128.546e-1643.75gene:FBgn0040623 transcript:FBtr0085435[more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spcs11.356e-1348.84signal peptidase complex subunit 1 [Source:ZFIN;Ac... [more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrpl441.236e-743.06mitochondrial ribosomal protein L44 [Source:Xenbas... [more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Spcs11.577e-1038.89signal peptidase complex subunit 1 homolog (S. cer... [more]
Spcs17.277e-1038.89signal peptidase complex subunit 1 homolog (S. cer... [more]
Spcs17.277e-1038.89signal peptidase complex subunit 1 homolog (S. cer... [more]
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BLAST of signal peptidase complex subunit 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VAL0|SPCS1_DROME8.566e-1543.75Signal peptidase complex subunit 1 OS=Drosophila m... [more]
sp|Q3T134|SPCS1_BOVIN5.481e-1038.37Signal peptidase complex subunit 1 OS=Bos taurus O... [more]
sp|B0FWK4|SPCS1_PIG1.035e-938.37Signal peptidase complex subunit 1 OS=Sus scrofa O... [more]
sp|Q5RF96|SPCS1_PONAB1.740e-939.53Signal peptidase complex subunit 1 OS=Pongo abelii... [more]
sp|Q9Y6A9|SPCS1_HUMAN2.032e-939.53Signal peptidase complex subunit 1 OS=Homo sapiens... [more]
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BLAST of signal peptidase complex subunit 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GKY11.318e-1747.31Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F4N61.406e-1747.31Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A2H1V8V75.305e-1748.81SFRICE_004166 OS=Spodoptera frugiperda OX=7108 GN=... [more]
A0A5N4A7E35.785e-1748.28Uncharacterized protein OS=Photinus pyralis OX=705... [more]
A0A1D2AIC29.555e-1754.43Signal peptidase complex subunit 1 (Fragment) OS=O... [more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spcs12.129e-1147.37signal peptidase complex subunit 1 [Source:NCBI ge... [more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO384821.521e-1647.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of signal peptidase complex subunit 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
spcs17.976e-1247.62signal peptidase complex subunit 1 [Source:NCBI ge... [more]
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BLAST of signal peptidase complex subunit 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000027186.14.651e-55100.00SMESG000027186.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004516 ID=SMED30004516|Name=signal peptidase complex subunit 1|organism=Schmidtea mediterranea sexual|type=transcript|length=464bp
AATAATGTTTGAGATATCTGTTGTTTGAACTATTTAATAAATAAATATTC
AACGTTTATATATTAAAATTATGAATTATTTTCAAAATATCCAAGATAGA
ATAGAAAGCATTCCTCATCATTTGGATTTTATTGGACAAAAACATGCTGA
AAAAATATTTCAATGTATCATAGTTATTACTGGGATAATTGGATTTATCT
GGGGATATTATTGTCAACAGATATCTCAAACATTATTAGTACTGGCAGTT
GGATTTGTTCTAAGCTTATTTTTGGTTGTTATACCATGGCCGTTCTATAG
AAAAAATTCTTTAAATTGGCAGAAAGTTTTAGAAAATGAAACCCAACAGG
AAACCGGTGATGTTAAAAAAGAAAAACCAGCCAATAAACCTAAAAAGAAT
AAAAAATCGGAAAAAAATATCTGATTTAAAAGAATTTTTATATTAATAAA
TGTTTTGGTTATTG
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protein sequence of SMED30004516-orf-1

>SMED30004516-orf-1 ID=SMED30004516-orf-1|Name=SMED30004516-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp
MNYFQNIQDRIESIPHHLDFIGQKHAEKIFQCIIVITGIIGFIWGYYCQQ
ISQTLLVLAVGFVLSLFLVVIPWPFYRKNSLNWQKVLENETQQETGDVKK
EKPANKPKKNKKSEKNI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR037713Spc1
IPR009542SPC12
Vocabulary: molecular function
TermDefinition
GO:0008233peptidase activity
Vocabulary: cellular component
TermDefinition
GO:0005787signal peptidase complex
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0006465signal peptide processing
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009542Microsomal signal peptidase 12kDa subunitPFAMPF06645SPC12coord: 18..86
e-value: 1.0E-25
score: 89.6
IPR009542Microsomal signal peptidase 12kDa subunitPANTHERPTHR13202MICROSOMAL SIGNAL PEPTIDASE 12 KDA SUBUNITcoord: 9..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 92..117
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..117
NoneNo IPR availableCDDcd18585ABC_6TM_CydCcoord: 33..78
e-value: 0.00323404
score: 33.6074
NoneNo IPR availableTMHMMTMhelixcoord: 55..77
NoneNo IPR availableTMHMMTMhelixcoord: 29..48
IPR037713Signal peptidase complex subunit Spc1PANTHERPTHR13202:SF0SIGNAL PEPTIDASE COMPLEX SUBUNIT 1coord: 9..112