E3 ubiquitin-protein ligase MIB2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004508 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Human
Match: MIB1 (mindbomb E3 ubiquitin protein ligase 1 [Source:HGNC Symbol;Acc:HGNC:21086]) HSP 1 Score: 281.952 bits (720), Expect = 6.457e-80 Identity = 206/732 (28.14%), Postives = 361/732 (49.32%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V S + + +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL+++ ++ A++++GD A+H A D+ + ++L AD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV GA++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DP+L + L+ + ++S ++ ++ S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANIVRSG-DAAQGAEGGTSQ--------FQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASG--ERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSR--------------------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 57.7658 bits (138), Expect = 1.087e-7 Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQY------DTRQ-------DHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + D Q ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397 HSP 3 Score: 51.6026 bits (122), Expect = 8.285e-6 Identity = 40/176 (22.73%), Postives = 75/176 (42.61%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXK------VMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGK 751
G+ YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + + V + + E +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSK 399
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 229.565 bits (584), Expect = 5.349e-62 Identity = 185/723 (25.59%), Postives = 325/723 (44.95%), Query Frame = 2
Query: 686 DGDMYVSRLGMEWMINPKCIGKRNENKFK--------------------SEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDR-LELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN----LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAES--VNALLD-AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQ----STASSNEGSDK---PEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
DG V R+G + ++ KC+G+ + S+ + GD V+ D ++++Q+ HGGW M G+ V I GDV+V + ++P LTK F GD VR+I + +KRLQ GHGEW + M LG+VGKV V +G+L+V + GQ+ +P C+ A + ++ D+ + + + + + D + + +GN L+ +R+ + V T +G L +A G +++++LL++ RA ++ ++ G+TALH A ++ + +L++ G +A N+ + T + +A G +++ R L R D+++ ++ +DT LH A+ S V L + D+ A N++ F H + +G+ + L R ++V+++K+D F ALHLA NNH ++L++ + D+N+RN LQ+ LH+AV Q ++ + +LV+ G ++ D GDT LH+A++ H + + G G G Q AS GS + +A +L + A N ++P+D K A+R + A GG A PG Q L T + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 219 DGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-------------------------FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKN--QGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALC-ERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGA-GGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRER-------------QAGGG--AAPGPR------QTLGTPN-TVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 889 HSP 2 Score: 61.6178 bits (148), Expect = 7.006e-9 Identity = 36/144 (25.00%), Postives = 67/144 (46.53%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQY--DTR-----------QDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D + ++ Q HGGWN M E + G +G V+R+ ++ +Q+ +TR +G V +I + + KRLQ +GEW+DDM LG+ ++++ F DGN+++ G+ + + ++
Sbjct: 282 DTDVLREMQEGHGGWNPRMAEFI-----------------GQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVA 408
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 230.335 bits (586), Expect = 5.809e-62 Identity = 190/744 (25.54%), Postives = 332/744 (44.62%), Query Frame = 2
Query: 623 GWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFK--------------------SEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDR-LELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN----LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAES--VNALLD-AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQ----STASSNEGSDK---PEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
GW ++ G V V DG V R+G + ++ KC+G+ + S+ + GD V+ D ++++Q+ HGGW M G+ V I GDV+V + ++P LTK F GD VR+I + +KRLQ GHGEW + M LG+VGKV V +G+L+V + GQ+ +P C+ A + ++ D+ + + + + + D + + +GN L+ +R+ + V T +G L +A G +++++LL++ RA ++ ++ G+TALH A ++ + +L++ G +A N+ + T + +A G +++ R L R D+++ ++ +DT LH A+ S V L + D+ A N++ F H + +G+ + L R ++V+++K+D F ALHLA NNH ++L++ + D+N+RN LQ+ LH+AV Q ++ + +LV+ G ++ D GDT LH+A++ H + + G G G Q AS GS + +A +L + A N ++P+D K A+R + A GG A PG Q L T + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 290 GW----DVETGRSVASV-TWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-------------------------FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKN--QGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRAL-CERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGA-GGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRER-------------QAGGG--AAPGPR------QTLGTPN-TVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 976 HSP 2 Score: 61.6178 bits (148), Expect = 6.787e-9 Identity = 36/144 (25.00%), Postives = 67/144 (46.53%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQY--DTR-----------QDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D + ++ Q HGGWN M E + G +G V+R+ ++ +Q+ +TR +G V +I + + KRLQ +GEW+DDM LG+ ++++ F DGN+++ G+ + + ++
Sbjct: 369 DTDVLREMQEGHGGWNPRMAEFI-----------------GQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVA 495
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 229.18 bits (583), Expect = 1.244e-61 Identity = 190/744 (25.54%), Postives = 332/744 (44.62%), Query Frame = 2
Query: 623 GWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFK--------------------SEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDR-LELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN----LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAES--VNALLD-AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQ----STASSNEGSDK---PEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
GW ++ G V V DG V R+G + ++ KC+G+ + S+ + GD V+ D ++++Q+ HGGW M G+ V I GDV+V + ++P LTK F GD VR+I + +KRLQ GHGEW + M LG+VGKV V +G+L+V + GQ+ +P C+ A + ++ D+ + + + + + D + + +GN L+ +R+ + V T +G L +A G +++++LL++ RA ++ ++ G+TALH A ++ + +L++ G +A N+ + T + +A G +++ R L R D+++ ++ +DT LH A+ S V L + D+ A N++ F H + +G+ + L R ++V+++K+D F ALHLA NNH ++L++ + D+N+RN LQ+ LH+AV Q ++ + +LV+ G ++ D GDT LH+A++ H + + G G G Q AS GS + +A +L + A N ++P+D K A+R + A GG A PG Q L T + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 304 GW----DVETGRSVASV-TWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-------------------------FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKN--QGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRAL-CERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGA-GGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRER-------------QAGGG--AAPGPR------QTLGTPN-TVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 990 HSP 2 Score: 61.6178 bits (148), Expect = 7.741e-9 Identity = 36/144 (25.00%), Postives = 67/144 (46.53%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQY--DTR-----------QDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D + ++ Q HGGWN M E + G +G V+R+ ++ +Q+ +TR +G V +I + + KRLQ +GEW+DDM LG+ ++++ F DGN+++ G+ + + ++
Sbjct: 383 DTDVLREMQEGHGGWNPRMAEFI-----------------GQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVA 509
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Human
Match: MIB2 (mindbomb E3 ubiquitin protein ligase 2 [Source:HGNC Symbol;Acc:HGNC:30577]) HSP 1 Score: 226.868 bits (577), Expect = 4.894e-61 Identity = 177/674 (26.26%), Postives = 310/674 (45.99%), Query Frame = 2
Query: 773 SEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDR-LELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN----LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAES--VNALLD-AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQ----STASSNEGSDK---PEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
S+ + GD V+ D ++++Q+ HGGW M G+ V I GDV+V + ++P LTK F GD VR+I + +KRLQ GHGEW + M LG+VGKV V +G+L+V + GQ+ +P C+ A + ++ D+ + + + + + D + + +GN L+ +R+ + V T +G L +A G +++++LL++ RA ++ ++ G+TALH A ++ + +L++ G +A N+ + T + +A G +++ R L R D+++ ++ +DT LH A+ S V L + D+ A N++ F H + +G+ + L R ++V+++K+D F ALHLA NNH ++L++ + D+N+RN LQ+ LH+AV Q ++ + +LV+ G ++ D GDT LH+A++ H + + G G G Q AS GS + +A +L + A N ++P+D K A+R + A GG A PG Q L T + + L + C++C + + V F C H C+ CA + +C C+ + K+R
Sbjct: 304 SQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHS-------------------------FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKN--QGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRAL-CERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGA-GGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRER-------------QAGGG--AAPGPR------QTLGTPN-TVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLR 925 HSP 2 Score: 61.6178 bits (148), Expect = 6.626e-9 Identity = 36/144 (25.00%), Postives = 67/144 (46.53%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQY--DTR-----------QDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D + ++ Q HGGWN M E + G +G V+R+ ++ +Q+ +TR +G V +I + + KRLQ +GEW+DDM LG+ ++++ F DGN+++ G+ + + ++
Sbjct: 318 DTDVLREMQEGHGGWNPRMAEFI-----------------GQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVA 444
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 128.257 bits (321), Expect = 1.231e-29 Identity = 77/252 (30.56%), Postives = 131/252 (51.98%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
G PL IA + GH +V +LL+EK AN+N + + + LH+A + + + +LL+++GA ++++ + +TP+ AA +G + LL + A +S LH+A + H ++ LL A + V H+ A G+ R+ ++ L R NS+ + F LH+A N + +LLKY+A I + T LH+A +N + L+++GA DV G+TPLH+A R + D V+
Sbjct: 227 GFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVR 475 HSP 2 Score: 124.02 bits (310), Expect = 2.151e-28 Identity = 96/311 (30.87%), Postives = 145/311 (46.62%), Query Frame = 2
Query: 1325 QIMGQSDHSDESDE------PDNDRTKTFFNALKI----GNLNEVRKLVDSVG--TSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRN-ADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMD 2218
+ Q DH D + P +D T + L + G++ + L+D S L G PL IA I VV+LL++ RA I A E+G T LH+A I L+ +GA+ + + TP+ LAA A D+ R+L RN A + E T LH+A + + + V LL AGA+ A N++ H+ A+ G E + + L K + ++K F LHLA + +R+LL+ ++I N T LH+A +L+E GA NG TPLHIA + + M+
Sbjct: 331 HMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI--RNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQME 637 HSP 3 Score: 122.865 bits (307), Expect = 5.272e-28 Identity = 94/327 (28.75%), Postives = 156/327 (47.71%), Query Frame = 2
Query: 1430 RKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDIT-RLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN-------EGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
R VD V L PL +A + GH+ V KLL+++ A+ N++ NG T LHIA +K++ K+ +LL+ A + A + +TP+ +AA G+++I LL N D+ E T LHLA + + V L+ GA + A + H+ ++ GN I+ + L+ N+ D ++ LH+A E +LL + AD + T LH+A L +++L+E+G +D+ N TPLH+A ++ K LL +G A+ N ++A LL+ KA + K++ TP+
Sbjct: 349 RAPVDDVTVDYLT----PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN----DKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLS 663 HSP 4 Score: 121.324 bits (303), Expect = 1.558e-27 Identity = 83/266 (31.20%), Postives = 135/266 (50.75%), Query Frame = 2
Query: 1436 LVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
L+D TLL +G PL +A G+++VV+LL+E+ ++ + +N T LH+A + K+ LL+ GA A N TP+ +AA M+I L + AD + + T LHL+ + H E L++ G+D+ A N A HL AQ + + ++ L G +I NS+ + + LH+A ++ L++ AD+ + T LH A Q + NC++ L+E GA + A G TPL IA R+ + V+
Sbjct: 543 LLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK-ADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQI-LYNNGAEI-NSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVE 805 HSP 5 Score: 110.538 bits (275), Expect = 3.398e-24 Identity = 79/305 (25.90%), Postives = 139/305 (45.57%), Query Frame = 2
Query: 1481 PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK---KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
PL IA G+ D+V LL++ AN NA + + LHIA ++ + ++ +L++ AD TP+ LA+ G++++ RLL R + I T LH+A + + LL+ GA +A + H+ A++ I L+ + N++ F LHL+ H+ +L++ +D+ + NN TA+H+ + ++ +IL GAEI+ G TPLH+A ++ VK + G +G T + YLL+ A + + +TP+
Sbjct: 494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 6 Score: 110.153 bits (274), Expect = 4.538e-24 Identity = 81/279 (29.03%), Postives = 141/279 (50.54%), Query Frame = 2
Query: 1382 TKTFFNALKIGNLNEVRKLVDSVGT---STLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+ +F A + G+L +V +L+ + GT ++ G+ L +A GH +VV+ LI+++A ++A G+TALHIA + I +L+ GA+VN Q+ N TP+ +AA ++ + L A+ ++ + T L +A++ H V LL D+ +R A H+ A++ + + L+ E V S+ F LH+A HE ++LL+ A++N + + + LH+A N +L+ +GA ID + TPLH A R
Sbjct: 34 SASFLRAARAGDLEKVLELLRA-GTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYL-LKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVR------LPALHIAAKKDDTTAATLLLQNEHNPDVTSKSG--FTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAAR 302 HSP 7 Score: 56.6102 bits (135), Expect = 1.057e-7 Identity = 40/117 (34.19%), Postives = 62/117 (52.99%), Query Frame = 2
Query: 1880 AFHLVAQRGN-ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+F A+ G+ E++LE+ R G I N+ + +LHLA H +R L+K +A ++ TALHIA + ILVE GA ++V NG TPL++A + + + VK
Sbjct: 36 SFLRAARAGDLEKVLELL--RAGTDI-NTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVK 149
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 128.257 bits (321), Expect = 1.231e-29 Identity = 77/252 (30.56%), Postives = 131/252 (51.98%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
G PL IA + GH +V +LL+EK AN+N + + + LH+A + + + +LL+++GA ++++ + +TP+ AA +G + LL + A +S LH+A + H ++ LL A + V H+ A G+ R+ ++ L R NS+ + F LH+A N + +LLKY+A I + T LH+A +N + L+++GA DV G+TPLH+A R + D V+
Sbjct: 227 GFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVR 475 HSP 2 Score: 124.02 bits (310), Expect = 2.151e-28 Identity = 96/311 (30.87%), Postives = 145/311 (46.62%), Query Frame = 2
Query: 1325 QIMGQSDHSDESDE------PDNDRTKTFFNALKI----GNLNEVRKLVDSVG--TSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRN-ADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMD 2218
+ Q DH D + P +D T + L + G++ + L+D S L G PL IA I VV+LL++ RA I A E+G T LH+A I L+ +GA+ + + TP+ LAA A D+ R+L RN A + E T LH+A + + + V LL AGA+ A N++ H+ A+ G E + + L K + ++K F LHLA + +R+LL+ ++I N T LH+A +L+E GA NG TPLHIA + + M+
Sbjct: 331 HMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI--RNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQME 637 HSP 3 Score: 122.865 bits (307), Expect = 5.272e-28 Identity = 94/327 (28.75%), Postives = 156/327 (47.71%), Query Frame = 2
Query: 1430 RKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDIT-RLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN-------EGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
R VD V L PL +A + GH+ V KLL+++ A+ N++ NG T LHIA +K++ K+ +LL+ A + A + +TP+ +AA G+++I LL N D+ E T LHLA + + V L+ GA + A + H+ ++ GN I+ + L+ N+ D ++ LH+A E +LL + AD + T LH+A L +++L+E+G +D+ N TPLH+A ++ K LL +G A+ N ++A LL+ KA + K++ TP+
Sbjct: 349 RAPVDDVTVDYLT----PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN----DKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLS 663 HSP 4 Score: 121.324 bits (303), Expect = 1.558e-27 Identity = 83/266 (31.20%), Postives = 135/266 (50.75%), Query Frame = 2
Query: 1436 LVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
L+D TLL +G PL +A G+++VV+LL+E+ ++ + +N T LH+A + K+ LL+ GA A N TP+ +AA M+I L + AD + + T LHL+ + H E L++ G+D+ A N A HL AQ + + ++ L G +I NS+ + + LH+A ++ L++ AD+ + T LH A Q + NC++ L+E GA + A G TPL IA R+ + V+
Sbjct: 543 LLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK-ADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQI-LYNNGAEI-NSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVE 805 HSP 5 Score: 110.538 bits (275), Expect = 3.398e-24 Identity = 79/305 (25.90%), Postives = 139/305 (45.57%), Query Frame = 2
Query: 1481 PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK---KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
PL IA G+ D+V LL++ AN NA + + LHIA ++ + ++ +L++ AD TP+ LA+ G++++ RLL R + I T LH+A + + LL+ GA +A + H+ A++ I L+ + N++ F LHL+ H+ +L++ +D+ + NN TA+H+ + ++ +IL GAEI+ G TPLH+A ++ VK + G +G T + YLL+ A + + +TP+
Sbjct: 494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 6 Score: 110.153 bits (274), Expect = 4.538e-24 Identity = 81/279 (29.03%), Postives = 141/279 (50.54%), Query Frame = 2
Query: 1382 TKTFFNALKIGNLNEVRKLVDSVGT---STLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+ +F A + G+L +V +L+ + GT ++ G+ L +A GH +VV+ LI+++A ++A G+TALHIA + I +L+ GA+VN Q+ N TP+ +AA ++ + L A+ ++ + T L +A++ H V LL D+ +R A H+ A++ + + L+ E V S+ F LH+A HE ++LL+ A++N + + + LH+A N +L+ +GA ID + TPLH A R
Sbjct: 34 SASFLRAARAGDLEKVLELLRA-GTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYL-LKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVR------LPALHIAAKKDDTTAATLLLQNEHNPDVTSKSG--FTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAAR 302 HSP 7 Score: 56.6102 bits (135), Expect = 1.057e-7 Identity = 40/117 (34.19%), Postives = 62/117 (52.99%), Query Frame = 2
Query: 1880 AFHLVAQRGN-ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+F A+ G+ E++LE+ R G I N+ + +LHLA H +R L+K +A ++ TALHIA + ILVE GA ++V NG TPL++A + + + VK
Sbjct: 36 SFLRAARAGDLEKVLELL--RAGTDI-NTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVK 149
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 127.872 bits (320), Expect = 1.451e-29 Identity = 77/252 (30.56%), Postives = 131/252 (51.98%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
G PL IA + GH +V +LL+EK AN+N + + + LH+A + + + +LL+++GA ++++ + +TP+ AA +G + LL + A +S LH+A + H ++ LL A + V H+ A G+ R+ ++ L R NS+ + F LH+A N + +LLKY+A I + T LH+A +N + L+++GA DV G+TPLH+A R + D V+
Sbjct: 227 GFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVR 475 HSP 2 Score: 124.02 bits (310), Expect = 2.516e-28 Identity = 96/311 (30.87%), Postives = 145/311 (46.62%), Query Frame = 2
Query: 1325 QIMGQSDHSDESDE------PDNDRTKTFFNALKI----GNLNEVRKLVDSVG--TSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRN-ADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMD 2218
+ Q DH D + P +D T + L + G++ + L+D S L G PL IA I VV+LL++ RA I A E+G T LH+A I L+ +GA+ + + TP+ LAA A D+ R+L RN A + E T LH+A + + + V LL AGA+ A N++ H+ A+ G E + + L K + ++K F LHLA + +R+LL+ ++I N T LH+A +L+E GA NG TPLHIA + + M+
Sbjct: 331 HMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI--RNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQME 637 HSP 3 Score: 122.479 bits (306), Expect = 6.112e-28 Identity = 94/327 (28.75%), Postives = 156/327 (47.71%), Query Frame = 2
Query: 1430 RKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDIT-RLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN-------EGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
R VD V L PL +A + GH+ V KLL+++ A+ N++ NG T LHIA +K++ K+ +LL+ A + A + +TP+ +AA G+++I LL N D+ E T LHLA + + V L+ GA + A + H+ ++ GN I+ + L+ N+ D ++ LH+A E +LL + AD + T LH+A L +++L+E+G +D+ N TPLH+A ++ K LL +G A+ N ++A LL+ KA + K++ TP+
Sbjct: 349 RAPVDDVTVDYLT----PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN----DKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLS 663 HSP 4 Score: 120.939 bits (302), Expect = 1.984e-27 Identity = 83/266 (31.20%), Postives = 135/266 (50.75%), Query Frame = 2
Query: 1436 LVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
L+D TLL +G PL +A G+++VV+LL+E+ ++ + +N T LH+A + K+ LL+ GA A N TP+ +AA M+I L + AD + + T LHL+ + H E L++ G+D+ A N A HL AQ + + ++ L G +I NS+ + + LH+A ++ L++ AD+ + T LH A Q + NC++ L+E GA + A G TPL IA R+ + V+
Sbjct: 543 LLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK-ADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQI-LYNNGAEI-NSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVE 805 HSP 5 Score: 110.153 bits (274), Expect = 4.402e-24 Identity = 79/305 (25.90%), Postives = 139/305 (45.57%), Query Frame = 2
Query: 1481 PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK---KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
PL IA G+ D+V LL++ AN NA + + LHIA ++ + ++ +L++ AD TP+ LA+ G++++ RLL R + I T LH+A + + LL+ GA +A + H+ A++ I L+ + N++ F LHL+ H+ +L++ +D+ + NN TA+H+ + ++ +IL GAEI+ G TPLH+A ++ VK + G +G T + YLL+ A + + +TP+
Sbjct: 494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 6 Score: 109.768 bits (273), Expect = 5.446e-24 Identity = 81/279 (29.03%), Postives = 141/279 (50.54%), Query Frame = 2
Query: 1382 TKTFFNALKIGNLNEVRKLVDSVGT---STLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+ +F A + G+L +V +L+ + GT ++ G+ L +A GH +VV+ LI+++A ++A G+TALHIA + I +L+ GA+VN Q+ N TP+ +AA ++ + L A+ ++ + T L +A++ H V LL D+ +R A H+ A++ + + L+ E V S+ F LH+A HE ++LL+ A++N + + + LH+A N +L+ +GA ID + TPLH A R
Sbjct: 34 SASFLRAARAGDLEKVLELLRA-GTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYL-LKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVR------LPALHIAAKKDDTTAATLLLQNEHNPDVTSKSG--FTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAAR 302 HSP 7 Score: 56.225 bits (134), Expect = 1.292e-7 Identity = 40/117 (34.19%), Postives = 62/117 (52.99%), Query Frame = 2
Query: 1880 AFHLVAQRGN-ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+F A+ G+ E++LE+ R G I N+ + +LHLA H +R L+K +A ++ TALHIA + ILVE GA ++V NG TPL++A + + + VK
Sbjct: 36 SFLRAARAGDLEKVLELL--RAGTDI-NTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVK 149
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 127.872 bits (320), Expect = 1.451e-29 Identity = 77/252 (30.56%), Postives = 131/252 (51.98%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
G PL IA + GH +V +LL+EK AN+N + + + LH+A + + + +LL+++GA ++++ + +TP+ AA +G + LL + A +S LH+A + H ++ LL A + V H+ A G+ R+ ++ L R NS+ + F LH+A N + +LLKY+A I + T LH+A +N + L+++GA DV G+TPLH+A R + D V+
Sbjct: 227 GFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVR 475 HSP 2 Score: 124.02 bits (310), Expect = 2.516e-28 Identity = 96/311 (30.87%), Postives = 145/311 (46.62%), Query Frame = 2
Query: 1325 QIMGQSDHSDESDE------PDNDRTKTFFNALKI----GNLNEVRKLVDSVG--TSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRN-ADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMD 2218
+ Q DH D + P +D T + L + G++ + L+D S L G PL IA I VV+LL++ RA I A E+G T LH+A I L+ +GA+ + + TP+ LAA A D+ R+L RN A + E T LH+A + + + V LL AGA+ A N++ H+ A+ G E + + L K + ++K F LHLA + +R+LL+ ++I N T LH+A +L+E GA NG TPLHIA + + M+
Sbjct: 331 HMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI--RNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQME 637 HSP 3 Score: 122.479 bits (306), Expect = 6.112e-28 Identity = 94/327 (28.75%), Postives = 156/327 (47.71%), Query Frame = 2
Query: 1430 RKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDIT-RLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN-------EGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
R VD V L PL +A + GH+ V KLL+++ A+ N++ NG T LHIA +K++ K+ +LL+ A + A + +TP+ +AA G+++I LL N D+ E T LHLA + + V L+ GA + A + H+ ++ GN I+ + L+ N+ D ++ LH+A E +LL + AD + T LH+A L +++L+E+G +D+ N TPLH+A ++ K LL +G A+ N ++A LL+ KA + K++ TP+
Sbjct: 349 RAPVDDVTVDYLT----PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN----DKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLS 663 HSP 4 Score: 120.939 bits (302), Expect = 1.984e-27 Identity = 83/266 (31.20%), Postives = 135/266 (50.75%), Query Frame = 2
Query: 1436 LVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
L+D TLL +G PL +A G+++VV+LL+E+ ++ + +N T LH+A + K+ LL+ GA A N TP+ +AA M+I L + AD + + T LHL+ + H E L++ G+D+ A N A HL AQ + + ++ L G +I NS+ + + LH+A ++ L++ AD+ + T LH A Q + NC++ L+E GA + A G TPL IA R+ + V+
Sbjct: 543 LLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK-ADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQI-LYNNGAEI-NSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVE 805 HSP 5 Score: 110.153 bits (274), Expect = 4.402e-24 Identity = 79/305 (25.90%), Postives = 139/305 (45.57%), Query Frame = 2
Query: 1481 PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK---KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
PL IA G+ D+V LL++ AN NA + + LHIA ++ + ++ +L++ AD TP+ LA+ G++++ RLL R + I T LH+A + + LL+ GA +A + H+ A++ I L+ + N++ F LHL+ H+ +L++ +D+ + NN TA+H+ + ++ +IL GAEI+ G TPLH+A ++ VK + G +G T + YLL+ A + + +TP+
Sbjct: 494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 6 Score: 109.768 bits (273), Expect = 5.446e-24 Identity = 81/279 (29.03%), Postives = 141/279 (50.54%), Query Frame = 2
Query: 1382 TKTFFNALKIGNLNEVRKLVDSVGT---STLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+ +F A + G+L +V +L+ + GT ++ G+ L +A GH +VV+ LI+++A ++A G+TALHIA + I +L+ GA+VN Q+ N TP+ +AA ++ + L A+ ++ + T L +A++ H V LL D+ +R A H+ A++ + + L+ E V S+ F LH+A HE ++LL+ A++N + + + LH+A N +L+ +GA ID + TPLH A R
Sbjct: 34 SASFLRAARAGDLEKVLELLRA-GTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYL-LKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVR------LPALHIAAKKDDTTAATLLLQNEHNPDVTSKSG--FTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAAR 302 HSP 7 Score: 56.225 bits (134), Expect = 1.292e-7 Identity = 40/117 (34.19%), Postives = 62/117 (52.99%), Query Frame = 2
Query: 1880 AFHLVAQRGN-ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+F A+ G+ E++LE+ R G I N+ + +LHLA H +R L+K +A ++ TALHIA + ILVE GA ++V NG TPL++A + + + VK
Sbjct: 36 SFLRAARAGDLEKVLELL--RAGTDI-NTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVK 149
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 127.872 bits (320), Expect = 1.520e-29 Identity = 77/252 (30.56%), Postives = 131/252 (51.98%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
G PL IA + GH +V +LL+EK AN+N + + + LH+A + + + +LL+++GA ++++ + +TP+ AA +G + LL + A +S LH+A + H ++ LL A + V H+ A G+ R+ ++ L R NS+ + F LH+A N + +LLKY+A I + T LH+A +N + L+++GA DV G+TPLH+A R + D V+
Sbjct: 227 GFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVV-QGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADP--NSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVR 475 HSP 2 Score: 123.635 bits (309), Expect = 3.150e-28 Identity = 96/311 (30.87%), Postives = 145/311 (46.62%), Query Frame = 2
Query: 1325 QIMGQSDHSDESDE------PDNDRTKTFFNALKI----GNLNEVRKLVDSVG--TSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRN-ADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMD 2218
+ Q DH D + P +D T + L + G++ + L+D S L G PL IA I VV+LL++ RA I A E+G T LH+A I L+ +GA+ + + TP+ LAA A D+ R+L RN A + E T LH+A + + + V LL AGA+ A N++ H+ A+ G E + + L K + ++K F LHLA + +R+LL+ ++I N T LH+A +L+E GA NG TPLHIA + + M+
Sbjct: 331 HMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI--RNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKG--FTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQME 637 HSP 3 Score: 122.479 bits (306), Expect = 7.086e-28 Identity = 94/327 (28.75%), Postives = 156/327 (47.71%), Query Frame = 2
Query: 1430 RKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDIT-RLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN-------EGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
R VD V L PL +A + GH+ V KLL+++ A+ N++ NG T LHIA +K++ K+ +LL+ A + A + +TP+ +AA G+++I LL N D+ E T LHLA + + V L+ GA + A + H+ ++ GN I+ + L+ N+ D ++ LH+A E +LL + AD + T LH+A L +++L+E+G +D+ N TPLH+A ++ K LL +G A+ N ++A LL+ KA + K++ TP+
Sbjct: 349 RAPVDDVTVDYLT----PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGE--TPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANS--NATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNN----DKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLS 663 HSP 4 Score: 120.939 bits (302), Expect = 1.923e-27 Identity = 83/266 (31.20%), Postives = 135/266 (50.75%), Query Frame = 2
Query: 1436 LVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
L+D TLL +G PL +A G+++VV+LL+E+ ++ + +N T LH+A + K+ LL+ GA A N TP+ +AA M+I L + AD + + T LHL+ + H E L++ G+D+ A N A HL AQ + + ++ L G +I NS+ + + LH+A ++ L++ AD+ + T LH A Q + NC++ L+E GA + A G TPL IA R+ + V+
Sbjct: 543 LLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK-ADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQI-LYNNGAEI-NSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVE 805 HSP 5 Score: 110.153 bits (274), Expect = 4.638e-24 Identity = 79/305 (25.90%), Postives = 139/305 (45.57%), Query Frame = 2
Query: 1481 PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK---KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
PL IA G+ D+V LL++ AN NA + + LHIA ++ + ++ +L++ AD TP+ LA+ G++++ RLL R + I T LH+A + + LL+ GA +A + H+ A++ I L+ + N++ F LHL+ H+ +L++ +D+ + NN TA+H+ + ++ +IL GAEI+ G TPLH+A ++ VK + G +G T + YLL+ A + + +TP+
Sbjct: 494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLL-LERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADP--NAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIA 795 HSP 6 Score: 109.768 bits (273), Expect = 5.360e-24 Identity = 81/279 (29.03%), Postives = 141/279 (50.54%), Query Frame = 2
Query: 1382 TKTFFNALKIGNLNEVRKLVDSVGT---STLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+ +F A + G+L +V +L+ + GT ++ G+ L +A GH +VV+ LI+++A ++A G+TALHIA + I +L+ GA+VN Q+ N TP+ +AA ++ + L A+ ++ + T L +A++ H V LL D+ +R A H+ A++ + + L+ E V S+ F LH+A HE ++LL+ A++N + + + LH+A N +L+ +GA ID + TPLH A R
Sbjct: 34 SASFLRAARAGDLEKVLELLRA-GTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYL-LKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLENDSKGKVR------LPALHIAAKKDDTTAATLLLQNEHNPDVTSKSG--FTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAAR 302 HSP 7 Score: 56.225 bits (134), Expect = 1.202e-7 Identity = 40/117 (34.19%), Postives = 62/117 (52.99%), Query Frame = 2
Query: 1880 AFHLVAQRGN-ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+F A+ G+ E++LE+ R G I N+ + +LHLA H +R L+K +A ++ TALHIA + ILVE GA ++V NG TPL++A + + + VK
Sbjct: 36 SFLRAARAGDLEKVLELL--RAGTDI-NTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVK 149
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Fly
Match: mib1 (gene:FBgn0263601 transcript:FBtr0333578) HSP 1 Score: 271.166 bits (692), Expect = 5.903e-76 Identity = 201/699 (28.76%), Postives = 336/699 (48.07%), Query Frame = 2
Query: 791 GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRL-ELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECV----EATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDS----------------VGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVK-IVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK-----------------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKT-----CIICYDRPINVTF-GCGHVA-CDVCANNLYECHICRESIENKIRL 2743
GD V + D++ ++ LQ HGGW + M C V I D+ V + +NP +LTK ++PT A + F GD V++ ++ IK LQ+GHGEW +AMQ LGK+G+V+ V + DLKV++ NP V +T SD P I + +L+ I+ + + S D T+ F A G + + V + + G L A GHI+V+++L+ ++ ++++GD A+H A D+ + ++L GAD+NA+N + T + +A + G +++ + L T S+ +SE DT LH A+ H E ++ LLD GADI NN FNA H A +GN +++ L + IV +KDD + ALHLA NNH +L+ KA+++ +N NLQTALH+AV ++++ +K+LV+ GA++++ D +GDTPLH A+R H++ +K+ + G + ++N +A +L A +KN+ +TP+D C DP+L + L R + + E L + L A S+ + + E+ Q + + + + C++C D + F CGHV+ C+ CA + +C ICRE++ ++ ++
Sbjct: 232 GDKVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKV----SSPTTAPPE--------------FQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSS-ERLSAILKKLFEPNVS----GDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLT-LGCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLLMGLRNANN-KKASASIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVKCYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAA--SSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKI 903 HSP 2 Score: 96.6709 bits (239), Expect = 5.415e-20 Identity = 54/171 (31.58%), Postives = 87/171 (50.88%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSR-LGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSP 1075
KV + + E++++LQ HGGW + M + +V I +D D+ V+ G W NP + K + + + GDIV+IC D++ IK LQ+ HG W ++M+ GKI +VQ + D+KV V YNP+ + K + A+ + S L + K+F P
Sbjct: 234 KVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEP 404 HSP 3 Score: 64.6994 bits (156), Expect = 3.818e-10 Identity = 22/62 (35.48%), Postives = 45/62 (72.58%), Query Frame = 2
Query: 2561 EELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
+++++L+Q++QD++++ C +C+DR N+ F CGH C +C + + C ICR+++E +I L+
Sbjct: 1054 DDVQKLKQQLQDIKEQTMCPVCFDRIKNMVFLCGHGTCQMCGDQIEGCPICRKTVEKRILLF 1115 HSP 4 Score: 60.077 bits (144), Expect = 1.058e-8 Identity = 40/141 (28.37%), Postives = 66/141 (46.81%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQ-SLGSCAIVKGPSGNVYRLNSELLIQYDTR----QDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTS 532
D+E ++ Q HGGW M ECL+ +V +V SGN + N +L + + + ++G V + + E K LQ +GEW+D M LGK + Q + D ++K++ ++ N A+ CK S
Sbjct: 240 DLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAV----CKVAS 376
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Fly
Match: mib1 (gene:FBgn0263601 transcript:FBtr0075520) HSP 1 Score: 271.166 bits (692), Expect = 1.275e-75 Identity = 201/699 (28.76%), Postives = 336/699 (48.07%), Query Frame = 2
Query: 791 GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRL-ELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECV----EATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDS----------------VGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVK-IVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK-----------------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKT-----CIICYDRPINVTF-GCGHVA-CDVCANNLYECHICRESIENKIRL 2743
GD V + D++ ++ LQ HGGW + M C V I D+ V + +NP +LTK ++PT A + F GD V++ ++ IK LQ+GHGEW +AMQ LGK+G+V+ V + DLKV++ NP V +T SD P I + +L+ I+ + + S D T+ F A G + + V + + G L A GHI+V+++L+ ++ ++++GD A+H A D+ + ++L GAD+NA+N + T + +A + G +++ + L T S+ +SE DT LH A+ H E ++ LLD GADI NN FNA H A +GN +++ L + IV +KDD + ALHLA NNH +L+ KA+++ +N NLQTALH+AV ++++ +K+LV+ GA++++ D +GDTPLH A+R H++ +K+ + G + ++N +A +L A +KN+ +TP+D C DP+L + L R + + E L + L A S+ + + E+ Q + + + + C++C D + F CGHV+ C+ CA + +C ICRE++ ++ ++
Sbjct: 343 GDKVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKV----SSPTTAPPE--------------FQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSS-ERLSAILKKLFEPNVS----GDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLT-LGCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLLMGLRNANN-KKASASIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVKCYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAA--SSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKI 1014 HSP 2 Score: 96.6709 bits (239), Expect = 6.260e-20 Identity = 54/171 (31.58%), Postives = 87/171 (50.88%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSR-LGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSP 1075
KV + + E++++LQ HGGW + M + +V I +D D+ V+ G W NP + K + + + GDIV+IC D++ IK LQ+ HG W ++M+ GKI +VQ + D+KV V YNP+ + K + A+ + S L + K+F P
Sbjct: 345 KVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEP 515 HSP 3 Score: 65.0846 bits (157), Expect = 3.339e-10 Identity = 22/62 (35.48%), Postives = 45/62 (72.58%), Query Frame = 2
Query: 2561 EELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
+++++L+Q++QD++++ C +C+DR N+ F CGH C +C + + C ICR+++E +I L+
Sbjct: 1165 DDVQKLKQQLQDIKEQTMCPVCFDRIKNMVFLCGHGTCQMCGDQIEGCPICRKTVEKRILLF 1226 HSP 4 Score: 60.077 bits (144), Expect = 1.160e-8 Identity = 40/141 (28.37%), Postives = 66/141 (46.81%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQ-SLGSCAIVKGPSGNVYRLNSELLIQYDTR----QDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTS 532
D+E ++ Q HGGW M ECL+ +V +V SGN + N +L + + + ++G V + + E K LQ +GEW+D M LGK + Q + D ++K++ ++ N A+ CK S
Sbjct: 351 DLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAV----CKVAS 487
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0336840) HSP 1 Score: 210.305 bits (534), Expect = 9.240e-56 Identity = 162/541 (29.94%), Postives = 259/541 (47.87%), Query Frame = 2
Query: 791 GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLE--LVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIM--GQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVD--SVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDA-GADIRAVNNKNFNAFHLVAQRGN----ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPED-----LAIYLLKKKARTDIKNKN 2362
GD V++C ++ + +LQQ HGGW M K+ V I GD++V + ++P L K V F GD V +IN+ +K+++LQKGHGEW+ M+ LGK+ KV V +GDL+++ + G + LNP+CV+ S + + + L+ + S + G+L+ VR+ +D + G + +A + G++D+V LI K AN+NA ++ GD+ALH A ++ +L+ GA+VN N++ + + + A + R L NA+++I +S DTALH A+ + E V L +A D NN+ FN H A +GN RIL L R+ +VN +KDD FAALHLA N H + L+ + +A+++IRNN QT +AVSQ + ++ LV +++ D +GD +H+ V S + I + S S +PED + IYL + R ++ N N
Sbjct: 256 GDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVK-------------------------VVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPLATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVREL-LQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRIL--LLSRQ---LVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNNAN 765 HSP 2 Score: 108.227 bits (269), Expect = 1.726e-23 Identity = 129/541 (23.84%), Postives = 230/541 (42.51%), Query Frame = 2
Query: 449 GNVKIKYR----GRTFRINSKAIL--TDNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSR--LGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV--DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVK----VFSPGDKVRLINNKHKIKR-LQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSD--CESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN-LNEVRKLVDSVGTSTLLEGVY--PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLIN-KGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAG-ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVF 2005
GNV +KY G + + +L T+ + + ++ +F G++V + + + LQ+ HGGW M + + + +I GD+ V W +P + K R GD+V I +D K++QLQ+ HG W+ M GKICKV + GD+++ D E NP KC + +P A + RS+++ L + V +P + + K+ R +GH ++V Q L++NP V+ C Q V+ ++ ++ + + + D+ + + + GN +R L+ L + L I + V+ L++ AN+N ++ GDTALH A+ K+ ++ +LL N D +NN + AA G++ R + +++ + ALHLA N HA+ V L+ G A++ NN+ F L +G+ ++E ++ VN++ +D A+HL V
Sbjct: 213 GNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL-------VKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNP----KCVKLERSPLATAAE--RSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVR---------------------------QYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGD----SALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSC--DVNAKDEDGDNAMHLCVI 707 HSP 3 Score: 82.8037 bits (203), Expect = 9.784e-16 Identity = 104/469 (22.17%), Postives = 197/469 (42.00%), Query Frame = 2
Query: 1493 AVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKG-ADVNAQNNNKITPIMLAASAGSMD-ITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLV----AQRGNERILE---MFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV----RIHSMDTVKKRGKLLGIS-GIFQSTASSNEGSDK------PEDLA--IYLLKKKARTDIKNKNDKT-----------------------------PMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
A+ G + ++L+ R +N ++++G ALH+A ++ + L+ +G A+++ +NN + TP +LA S G I RL+ S D++ + + D A+HL V + L + A+ + + F+L + R +R++ ++L R G ++ + ++ A++ + + H +++ + + NLQ + VS + + GAE +NG P +A + + + + K + I G ST S++ G K P++++ + KK + + + T PM +P + RM K + + + T +G ++A P + L + D L L ++ + E+ C IC +R +V F CGH AC CA L CH+CR++I KI LY
Sbjct: 605 ALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLS--CDVNAKDEDGDNAMHLCVIK------KSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRV---ELNNANASIFEWITDRH--IRQLIFGQQGEAESLPRNLQA---LEVSAGSADGEESSASAGAE-----SNGAGPGSVAAPPPPQRQTFELMPKPNDIPAIGVGASPSTPSASPGVKKLNSDATPQNVSPQVAPRKKAPKPPVTTSSSSTALEAGAAGPSTSPVIVPGPHECIVCNEILPMVRFEPCQHQIACEECGIRMKKCLRCAVAIERRLTV--SGRVVALPTSTSSPSDPTRLPSGDL-LRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY 1049 HSP 4 Score: 53.5286 bits (127), Expect = 1.141e-6 Identity = 33/123 (26.83%), Postives = 64/123 (52.03%), Query Frame = 2
Query: 149 QREHGGWNANMNECLT--GPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY--RGRTFRINSKAI 505
Q+ HGGWN M E L+ G + G + N + + L++ + ++G VT+I +A + ++LQ +GEW + M + LGK ++++ + DG+++I+ G + +N K +
Sbjct: 272 QQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVS---FRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCV 391
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0081142) HSP 1 Score: 210.305 bits (534), Expect = 9.240e-56 Identity = 162/541 (29.94%), Postives = 259/541 (47.87%), Query Frame = 2
Query: 791 GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLE--LVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIM--GQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVD--SVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDA-GADIRAVNNKNFNAFHLVAQRGN----ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPED-----LAIYLLKKKARTDIKNKN 2362
GD V++C ++ + +LQQ HGGW M K+ V I GD++V + ++P L K V F GD V +IN+ +K+++LQKGHGEW+ M+ LGK+ KV V +GDL+++ + G + LNP+CV+ S + + + L+ + S + G+L+ VR+ +D + G + +A + G++D+V LI K AN+NA ++ GD+ALH A ++ +L+ GA+VN N++ + + + A + R L NA+++I +S DTALH A+ + E V L +A D NN+ FN H A +GN RIL L R+ +VN +KDD FAALHLA N H + L+ + +A+++IRNN QT +AVSQ + ++ LV +++ D +GD +H+ V S + I + S S +PED + IYL + R ++ N N
Sbjct: 256 GDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVK-------------------------VVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPLATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVREL-LQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRIL--LLSRQ---LVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNNAN 765 HSP 2 Score: 108.227 bits (269), Expect = 1.726e-23 Identity = 129/541 (23.84%), Postives = 230/541 (42.51%), Query Frame = 2
Query: 449 GNVKIKYR----GRTFRINSKAIL--TDNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSR--LGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV--DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVK----VFSPGDKVRLINNKHKIKR-LQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSD--CESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN-LNEVRKLVDSVGTSTLLEGVY--PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLIN-KGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAG-ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVF 2005
GNV +KY G + + +L T+ + + ++ +F G++V + + + LQ+ HGGW M + + + +I GD+ V W +P + K R GD+V I +D K++QLQ+ HG W+ M GKICKV + GD+++ D E NP KC + +P A + RS+++ L + V +P + + K+ R +GH ++V Q L++NP V+ C Q V+ ++ ++ + + + D+ + + + GN +R L+ L + L I + V+ L++ AN+N ++ GDTALH A+ K+ ++ +LL N D +NN + AA G++ R + +++ + ALHLA N HA+ V L+ G A++ NN+ F L +G+ ++E ++ VN++ +D A+HL V
Sbjct: 213 GNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL-------VKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNP----KCVKLERSPLATAAE--RSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVR---------------------------QYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGD----SALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSC--DVNAKDEDGDNAMHLCVI 707 HSP 3 Score: 82.8037 bits (203), Expect = 9.784e-16 Identity = 104/469 (22.17%), Postives = 197/469 (42.00%), Query Frame = 2
Query: 1493 AVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKG-ADVNAQNNNKITPIMLAASAGSMD-ITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLV----AQRGNERILE---MFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV----RIHSMDTVKKRGKLLGIS-GIFQSTASSNEGSDK------PEDLA--IYLLKKKARTDIKNKNDKT-----------------------------PMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
A+ G + ++L+ R +N ++++G ALH+A ++ + L+ +G A+++ +NN + TP +LA S G I RL+ S D++ + + D A+HL V + L + A+ + + F+L + R +R++ ++L R G ++ + ++ A++ + + H +++ + + NLQ + VS + + GAE +NG P +A + + + + K + I G ST S++ G K P++++ + KK + + + T PM +P + RM K + + + T +G ++A P + L + D L L ++ + E+ C IC +R +V F CGH AC CA L CH+CR++I KI LY
Sbjct: 605 ALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLS--CDVNAKDEDGDNAMHLCVIK------KSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRV---ELNNANASIFEWITDRH--IRQLIFGQQGEAESLPRNLQA---LEVSAGSADGEESSASAGAE-----SNGAGPGSVAAPPPPQRQTFELMPKPNDIPAIGVGASPSTPSASPGVKKLNSDATPQNVSPQVAPRKKAPKPPVTTSSSSTALEAGAAGPSTSPVIVPGPHECIVCNEILPMVRFEPCQHQIACEECGIRMKKCLRCAVAIERRLTV--SGRVVALPTSTSSPSDPTRLPSGDL-LRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY 1049 HSP 4 Score: 53.5286 bits (127), Expect = 1.141e-6 Identity = 33/123 (26.83%), Postives = 64/123 (52.03%), Query Frame = 2
Query: 149 QREHGGWNANMNECLT--GPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY--RGRTFRINSKAI 505
Q+ HGGWN M E L+ G + G + N + + L++ + ++G VT+I +A + ++LQ +GEW + M + LGK ++++ + DG+++I+ G + +N K +
Sbjct: 272 QQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVS---FRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCV 391
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Fly
Match: mib2 (gene:FBgn0086442 transcript:FBtr0336841) HSP 1 Score: 210.305 bits (534), Expect = 9.240e-56 Identity = 162/541 (29.94%), Postives = 259/541 (47.87%), Query Frame = 2
Query: 791 GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLE--LVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIM--GQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVD--SVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDA-GADIRAVNNKNFNAFHLVAQRGN----ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPED-----LAIYLLKKKARTDIKNKN 2362
GD V++C ++ + +LQQ HGGW M K+ V I GD++V + ++P L K V F GD V +IN+ +K+++LQKGHGEW+ M+ LGK+ KV V +GDL+++ + G + LNP+CV+ S + + + L+ + S + G+L+ VR+ +D + G + +A + G++D+V LI K AN+NA ++ GD+ALH A ++ +L+ GA+VN N++ + + + A + R L NA+++I +S DTALH A+ + E V L +A D NN+ FN H A +GN RIL L R+ +VN +KDD FAALHLA N H + L+ + +A+++IRNN QT +AVSQ + ++ LV +++ D +GD +H+ V S + I + S S +PED + IYL + R ++ N N
Sbjct: 256 GDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVK-------------------------VVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPLATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVREL-LQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRIL--LLSRQ---LVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNNAN 765 HSP 2 Score: 108.227 bits (269), Expect = 1.726e-23 Identity = 129/541 (23.84%), Postives = 230/541 (42.51%), Query Frame = 2
Query: 449 GNVKIKYR----GRTFRINSKAIL--TDNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSR--LGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV--DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVK----VFSPGDKVRLINNKHKIKR-LQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSD--CESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN-LNEVRKLVDSVGTSTLLEGVY--PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLIN-KGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAG-ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVF 2005
GNV +KY G + + +L T+ + + ++ +F G++V + + + LQ+ HGGW M + + + +I GD+ V W +P + K R GD+V I +D K++QLQ+ HG W+ M GKICKV + GD+++ D E NP KC + +P A + RS+++ L + V +P + + K+ R +GH ++V Q L++NP V+ C Q V+ ++ ++ + + + D+ + + + GN +R L+ L + L I + V+ L++ AN+N ++ GDTALH A+ K+ ++ +LL N D +NN + AA G++ R + +++ + ALHLA N HA+ V L+ G A++ NN+ F L +G+ ++E ++ VN++ +D A+HL V
Sbjct: 213 GNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAAL-------VKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNP----KCVKLERSPLATAAE--RSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVR---------------------------QYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGD----SALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSC--DVNAKDEDGDNAMHLCVI 707 HSP 3 Score: 82.8037 bits (203), Expect = 9.784e-16 Identity = 104/469 (22.17%), Postives = 197/469 (42.00%), Query Frame = 2
Query: 1493 AVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKG-ADVNAQNNNKITPIMLAASAGSMD-ITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLV----AQRGNERILE---MFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV----RIHSMDTVKKRGKLLGIS-GIFQSTASSNEGSDK------PEDLA--IYLLKKKARTDIKNKNDKT-----------------------------PMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
A+ G + ++L+ R +N ++++G ALH+A ++ + L+ +G A+++ +NN + TP +LA S G I RL+ S D++ + + D A+HL V + L + A+ + + F+L + R +R++ ++L R G ++ + ++ A++ + + H +++ + + NLQ + VS + + GAE +NG P +A + + + + K + I G ST S++ G K P++++ + KK + + + T PM +P + RM K + + + T +G ++A P + L + D L L ++ + E+ C IC +R +V F CGH AC CA L CH+CR++I KI LY
Sbjct: 605 ALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLS--CDVNAKDEDGDNAMHLCVIK------KSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRV---ELNNANASIFEWITDRH--IRQLIFGQQGEAESLPRNLQA---LEVSAGSADGEESSASAGAE-----SNGAGPGSVAAPPPPQRQTFELMPKPNDIPAIGVGASPSTPSASPGVKKLNSDATPQNVSPQVAPRKKAPKPPVTTSSSSTALEAGAAGPSTSPVIVPGPHECIVCNEILPMVRFEPCQHQIACEECGIRMKKCLRCAVAIERRLTV--SGRVVALPTSTSSPSDPTRLPSGDL-LRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY 1049 HSP 4 Score: 53.5286 bits (127), Expect = 1.141e-6 Identity = 33/123 (26.83%), Postives = 64/123 (52.03%), Query Frame = 2
Query: 149 QREHGGWNANMNECLT--GPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY--RGRTFRINSKAI 505
Q+ HGGWN M E L+ G + G + N + + L++ + ++G VT+I +A + ++LQ +GEW + M + LGK ++++ + DG+++I+ G + +N K +
Sbjct: 272 QQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVS---FRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCV 391
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 281.182 bits (718), Expect = 8.908e-80 Identity = 213/741 (28.74%), Postives = 366/741 (49.39%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK---------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++++N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ L+ + + P HI S + K + ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGSSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV------APAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 905 HSP 2 Score: 171.4 bits (433), Expect = 4.399e-43 Identity = 185/739 (25.03%), Postives = 309/739 (41.81%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEG---VYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALLDAG---ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + T A G+R L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S+ V+ L ++ HS + + D D + +A G+ V +++ G PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ G D++ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + M LG G + +A+S +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ + L + +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 259 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGER-----LSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAASQ---NGHVDVLKLLL---KHSVDLEAEDKDGDRAVHHA-SFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTLIMG--------LGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSR---------------------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 861 HSP 3 Score: 67.0106 bits (162), Expect = 9.901e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 970 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1030 HSP 4 Score: 56.225 bits (134), Expect = 2.288e-7 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQDHK-------------IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + + +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397 HSP 5 Score: 51.2174 bits (121), Expect = 7.637e-6 Identity = 53/241 (21.99%), Postives = 99/241 (41.08%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVM------LIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNS---MEFCSGKICKVQSILPSGDVKVN 937
G YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + M + + + +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K + Q+ ++K+ + Q++ G +N +G + KV+ IL DV VN
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQL---LKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 459
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 280.411 bits (716), Expect = 2.189e-79 Identity = 213/748 (28.48%), Postives = 368/748 (49.20%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVE-----------ATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK---------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V A+ + P + +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++++N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ L+ + + P HI S + K + ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGSSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVP--AERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 916 HSP 2 Score: 166.392 bits (420), Expect = 1.354e-41 Identity = 185/745 (24.83%), Postives = 308/745 (41.34%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSA------LEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEG---VYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALLDAG---ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + T A G S L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S+ V+ L ++ HS + + D D + +A G+ V +++ G PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ G D++ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + M LG G + +A+S +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ + L + +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 259 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAASQ---NGHVDVLKLLL---KHSVDLEAEDKDGDRAVHHA-SFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTLIMG--------LGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSR---------------------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 872 HSP 3 Score: 67.0106 bits (162), Expect = 9.444e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 981 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1041 HSP 4 Score: 56.225 bits (134), Expect = 2.003e-7 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQDHK-------------IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + + +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397 HSP 5 Score: 51.2174 bits (121), Expect = 7.527e-6 Identity = 39/176 (22.16%), Postives = 73/176 (41.48%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVM------LIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGK 751
G YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + M + + + +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTK 399
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Zebrafish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 278.1 bits (710), Expect = 1.171e-78 Identity = 213/749 (28.44%), Postives = 366/749 (48.87%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN----------------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK---------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++++N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ L+ + + P HI S + K + ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGSSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV------APAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTVRTEKCLKLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 913 HSP 2 Score: 172.94 bits (437), Expect = 1.201e-43 Identity = 185/739 (25.03%), Postives = 310/739 (41.95%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEG---VYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALLDAG---ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + T A G+R L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S+ V+ L ++ HS + + D D + +A G+ V +++ G PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ G D++ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + + K LG G + +A+S +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ + L + +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 259 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGER-----LSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAASQ---NGHVDVLKLLL---KHSVDLEAEDKDGDRAVHHA-SFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTVRTEKCLKLIMGLGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSR---------------------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 869 HSP 3 Score: 67.0106 bits (162), Expect = 1.072e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 978 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1038 HSP 4 Score: 55.8398 bits (133), Expect = 2.332e-7 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQDHK-------------IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + + +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397 HSP 5 Score: 51.2174 bits (121), Expect = 7.651e-6 Identity = 53/241 (21.99%), Postives = 99/241 (41.08%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVM------LIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNS---MEFCSGKICKVQSILPSGDVKVN 937
G YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + M + + + +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K + Q+ ++K+ + Q++ G +N +G + KV+ IL DV VN
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQL---LKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 459
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Zebrafish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:ZFIN;Acc:ZDB-GENE-050628-1]) HSP 1 Score: 179.874 bits (455), Expect = 4.200e-46 Identity = 182/679 (26.80%), Postives = 296/679 (43.59%), Query Frame = 2
Query: 773 SEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSM-EFCSGKICKVQSILPSGDVKVNVDRL-ELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCES--------EPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAV-KNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLG---ISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKL--SVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
S ++GD V+ ++ ++Q+Q+ HGGW M E G + I GDV+V + ++P LTK + S L + V LI V A LG+VGKV V +GDL+V GQ NP C+ A + ++ T+++ L +++ QS E D P + A G+ +VR+L+ D V +G L +A + GH++VVK+L++ ++I AK+E+GD ALH F I D + + Q+ + + +A + + I + + +S DT LH A+ K+F + + D NN+ FN H A +GN+ EM L R ++ + +K+D F+ALHLA NNH +LLK + DINIRNN QT L +AV+Q ++ + +LV +GA+++ D +GDT +H ++ + TV + G + S N + +A +L ++ A N K+P+D S + L S A R+ V+ G G +S + + T + L + + C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 198 SHSFQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEIRDGAL-----ITDRGDVRVQYSNIIRWTFHPGALTKARRTHTHTHTHTHTHTHQSYLAQ-----------VCLIG---------------VGAELLALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPGEVDANLMTVENASESSSTVISVLEKLLSQST---EQDNPG----RLVIEAAH-GSAAKVRELLQKHPDKVDIKN--QGKTALQVAAHQGHVEVVKVLLQANSSIEAKDEDGDAALHYTA----FGIADSRVITLNKAHYQHLKGLCSLHMAENR-TEHIVFQCVHVCVCVVCVQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILLKEGRCDINIRNNRNQTPLQLAVTQGHMALVALLVMEGADVNAEDEDGDTAMHTSLSRQQLTTVISSAEGDGAVLYGRLCSSGLMGNTELNVGAAIACFLAQEGADISYANHKGKSPLDLVTDSSVQTLIRSFAEKHRLH--------------AVSCG-----TGSSSSSSSLRRVHTTPNTMTNL--AVPSVTGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIRCQVTITKKIR 808 HSP 2 Score: 87.8113 bits (216), Expect = 4.075e-17 Identity = 158/769 (20.55%), Postives = 277/769 (36.02%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGM-EWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGN-QANPTAARGQRRRSSA----LEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLL-INKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAG-ADIRAVNNKNFNAFHLVAQRGNERILEMF---------------------LKREGVKIVNSQKDDKFAALH-----LAVFNNHE-------GCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR-IHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
KV + ++++ +Q+ HGGW N + E + I GD+ V + W +P + K + Q L Q V + G++ KV + GD++V +NP L+ G AN SS LEKL + + RL+ ++ HG + + KV + G +++ + ++ ++ +++ Q++ S E+ + D D + A I + R + + L+G+ L +A N V + + + ++ GDT LH A+ KD I ++L + D QNN + AA G+ T ++ + + + +ALHLA N H + LL G DI NN+N L +G+ ++ + L R+ + V S + A L+ + N E C L + ADI+ N+ ++ L + ++ EK V+ G + ++R +H+ + ++G + S +LA+ +L + + + M C R I KI Q Q + NS QH LE+L+ R + ME+ TC IC D I + F CGH +C C++ L C ICR++I +I+L+
Sbjct: 206 KVKCLLEVDILRQMQEGHGGW----NPKMAEIRDGALITDRGDVRVQYSNIIRWTFHPGALTKARRTHTHTHTHTHTHTHQ--------SYLAQVCLIGVGAELLALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPGEVDANLMTVENASESSSTVISVLEKLLSQSTEQDNPGRLV--------IEAAHGSAAKVRELLQKHPDKV-DIKNQGKTALQVAAHQGHV-----------------------EVVKVLLQANSSIEAKDEDGD-AALHYTAFGIA---DSRVITLNKAHYQHLKGLCSLHMAENRTEHIVFQCVHVCVCVVCVQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSRARQLADVKKEDGFSALHLAALNNHRDVAEILLKEGRCDINIRNNRNQTPLQLAVTQGHMALVALLVMEGADVNAEDEDGDTAMHTSLSRQQLTTVISSAEGDGAVLYGRLCSSGLMGNTELNVGAAIACF--LAQEGADISYANHKGKSPLDLVTDSSVQTLIRSFAEKHRLHAVSCGTGSSSSSSSLRRVHTTPNTMTNLAVPSVTGPSECLICS--------ELALLVLFSPCQHSVACEECAHRMKKCI-------------RCQVTITKKIRQDQTEVECSPS----------SENSEQH-----HLLEQLQSRYRQMEERITCPICIDNHIRLVFQCGHASCIDCSSALKTCPICRQTIRERIQLF 888
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 132.494 bits (332), Expect = 8.538e-31 Identity = 107/367 (29.16%), Postives = 181/367 (49.32%), Query Frame = 2
Query: 1391 FFNALKIGNLNEVRKLVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV---RIHSMDTVKKRGKLL------GISGIFQST---------------ASSNEGSDKPE------------DLAIYLLKKKARTDIKNKNDKTPM 2377
+ A + +L+ VR L+++ + ++ +G PL +A+ GH VV LL+E + K + ALHIA +KD K LL+ + + ++ + TP+ +AA G++++ LL +R A + + T LH+A K + V LLD G+ I A H A+ G+E+++EM L R G I++ K+ + LH+A +H C+++LL++ A ++ N+ TALH+A + K++V+K A + NG TPLHIA R+ M+ + K G L G++ I + AS N + + E D+ YLL+ A+ DIK K+D+T +
Sbjct: 145 LYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLL-LNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDR-GAPILSKTKNG-LSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTAL 504 HSP 2 Score: 122.094 bits (305), Expect = 1.658e-27 Identity = 77/241 (31.95%), Postives = 128/241 (53.11%), Query Frame = 2
Query: 1484 LMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGA--DIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV 2200
L +A + GH V K++++K+AN NAK NG T LHIA +K++ K+ +LL+ GA + A + +TPI +AA G +I + L T A + N +TALH+A + + V LL GA DI+A +++ A H+ ++ G I++ L++ + N+ + LHL+ H+ +LL+ + ++ T LH+A L +L++K A D +G TPLH+A
Sbjct: 372 LHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQL-THHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQ--TALHIASRLGKLEIVQQLLQKGALP--NAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAA 607 HSP 3 Score: 117.857 bits (294), Expect = 2.676e-26 Identity = 93/331 (28.10%), Postives = 157/331 (47.43%), Query Frame = 2
Query: 1409 IGNLNEVRKLVDSVGT--STLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV---RIHSMDTVKKRGKLLGI---SGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPM 2377
+G+ N V++L + +T + G L +A G IDVV+ L++ A ++ K ++ TALHIA + K +I L+ KGA NA + TP+ L+A G +I LL + + LS + T LH+A K E N LL A A H+ A N+R+ + L + +S + + LH+A N LL+Y A+ N + LH+A + ++ + +L+ K A +++ + NG TPLH+A + + + G + SG + + G+ K +A +LL+ +A+ + K KN TP+
Sbjct: 444 MGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALL-LEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASP--HSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMK---MANFLLENQAKPNAKTKNGYTPL 768 HSP 4 Score: 116.316 bits (290), Expect = 9.534e-26 Identity = 88/362 (24.31%), Postives = 178/362 (49.17%), Query Frame = 2
Query: 1328 IMGQSDHSDESDEPDNDRTKTFFNALKI----GNLNEVRKLVDSVGTSTLLE--GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK---KRGKL---LGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPM 2377
++ Q+DH+ + + +K+ F L I GN+N L++ + + PL +A G+ ++VKLL+++ + I AK ++G T LH + ++ ++L+++GA + ++ N ++P+ +A ++ +LL NA + ++ TALH+A H + ++D A+ A F H+ ++ +++E+ LK + + + +H+A F HE ++ L + A N N +TALH+A ++ ++ L++ GA++D+ + T LHIA R+ ++ V+ ++G L SG S+ EG +++A LL++ + K TP+
Sbjct: 221 LLLQNDHNADVE------SKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLL-LQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGAS--LQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGH---QEIAALLLEQGSSLSAATKKGFTPL 570 HSP 5 Score: 114.39 bits (285), Expect = 3.760e-25 Identity = 82/284 (28.87%), Postives = 143/284 (50.35%), Query Frame = 2
Query: 1373 NDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLE-----GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+D ++ A + GNL K++D + T + G+ L +A GH++VV L++ AN++A + G+TALHIA + ++ L+ GA+VNAQ+ N TP+ +AA +D+ R L N+ SI + T L +A++ H + V+ LL D +R A H+ A++ + + + L+ + V S+ F LH+A + +LL A ++ N T LH+A + N +K+L+++G++I+ +G TPLH R
Sbjct: 40 SDSNASYLRAARAGNL---EKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFL-LENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVR------LPALHIAARKDDTKSAALLLQNDHNADVESKSG--FTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGAR 311 HSP 6 Score: 113.62 bits (283), Expect = 6.378e-25 Identity = 81/298 (27.18%), Postives = 143/298 (47.99%), Query Frame = 2
Query: 1400 ALKIGNLNEVRKLVD--SVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNE 2287
A ++G L V++L+ ++ + G PL ++ GH ++ LL+E+ ++++A + G T LH+A + + ++ +LL+ K A +A + +TP+ +AA + + LL + A T LH+A K E LL+ GA+ V + + HL AQ G+ ++ + L ++ VN + LHLA ++ G VLL + A+I+ + + T LH+A + L+E A+ + NG TPLH A ++G I+ + Q AS NE
Sbjct: 507 ASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVA-LLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQAN--VNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAA---------QQGHTHIINMLLQYGASPNE 792 HSP 7 Score: 111.694 bits (278), Expect = 2.348e-24 Identity = 76/248 (30.65%), Postives = 127/248 (51.21%), Query Frame = 2
Query: 1394 FNALKIGNLNEVRKLV-DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKIL 2134
+ L++ NL +K D+ G S G+ PL +A + + V LL+++ A+ ++ +NG T LHIA +K++ +I L+ GA+ N I+P+ LAA GS+D+ LL T + A++++GN T LHLA ++ A LL+ GA+I A + H+ GN ++ L+ + N++ + + LH A H + +LL+Y A N N TAL IA Y++ + L
Sbjct: 576 YGQLEVANLLLQKKAAPDAAGKS----GLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLT-KQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKP--NAKTKNGYTPLHQAAQQGHTHIINMLLQYGASPNELTLNGNTALSIARRLGYISVVDTL 816
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182]) HSP 1 Score: 275.404 bits (703), Expect = 1.117e-77 Identity = 207/732 (28.28%), Postives = 358/732 (48.91%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L GD+V I +++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V S + + +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV A++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DP+L + L+ + + S ++ G H S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLLIGDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSG-DAAQGAEGGTSP--------FQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATG--ERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNLCKALAKCHKEKSSGQV----------------------GSH----SPSMISNDSETLEE-------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 166.777 bits (421), Expect = 1.469e-41 Identity = 198/799 (24.78%), Postives = 324/799 (40.55%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEG---VYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKI------------------------------------TPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNS---------------QKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK-----KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNK---NDKTPMDFCDPSLKEKLSVANARRMSKEIETK-ILQAQGPTTVAAGGLMAHP-GDHIDRNSAQHL-KTKD-----EELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + + + GD+VQIC D+++IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +++ + + A G+R L +L K+F + L N+ +K G V KV+ + + Q +++N +C T S+ ++ L + HS + + D D + +A G+ V +++ G PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + I +L+ GADV NNN T + LAA +++ LL NA+L I N TALHLAV+ H + V L+ A A + + H + L + V V++ Q +K +A +A F L AD+ IRN Q+ L + N K L + E +P I+ +++ KR L G G A+ + S + + I + ++RT I+ +DK P A M K ++ + +++ + P + G D I + Q L K KD ++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 259 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATGER-----LSQLLKKLFETQESGDL--NEELVKAAANG-------------DVAKVEDL---------LKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLK------HSVDVEAEDKDGDRAVHHA-AFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMGLGTQGAEKKSAASIACF---------LAANGADLTIRNKKGQSPLDLCPDP---NLCKALAKCHKEKSSGQVGSHSPSMISNDSETLEECMVCSDLKRDTLFGPCG---HIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFVMCCSGKGTEDVTDDIAGGNIQALQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1006 HSP 3 Score: 55.8398 bits (133), Expect = 3.412e-7 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQD-------------HKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
++E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 ELEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAV 397
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Xenopus
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:100170182]) HSP 1 Score: 275.018 bits (702), Expect = 2.578e-77 Identity = 209/743 (28.13%), Postives = 361/743 (48.59%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVE-----------ATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L GD+V I +++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V AT + + +V +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV A++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DP+L + L+ + + S ++ G H S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLLIGDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSG-DAAQGAEGGTSP--------FQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATGGKLDYNLSFK-LVERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNLCKALAKCHKEKSSGQV----------------------GSH----SPSMISNDSETLEE-------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 875 HSP 2 Score: 66.6254 bits (161), Expect = 1.682e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 986 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1046 HSP 3 Score: 55.8398 bits (133), Expect = 3.718e-7 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQD-------------HKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
++E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 ELEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAV 397
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 270.396 bits (690), Expect = 3.150e-76 Identity = 209/761 (27.46%), Postives = 355/761 (46.65%), Query Frame = 2
Query: 686 DGDMYVSRLGMEWMINPKCI----------------GKRNE--NKFKSEK--LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVD-RLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEAT----DSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDA-GADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGS---DKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKL--SVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDH---------IDRNSAQH-----------------------------LKTKDEE--LEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
DG V R+G + ++ KCI GK E K SE+ + GD V+ D++ ++++Q+ HGGW M G+ V I GDV+V + ++P LTK F GD VR+++N +K+LQ GHGEW + M LG++GKV V +GD++V + GQ NP C+ + D++ + + + N L I+ + S + D + + GN +VR+++ + +G L +A + G+++VVK+L++ ANI+ +++ GDTALH A ++ + +L++KGA+ NN K T + +A + G ++ ++L N +++ +S DT LH A+ + L + D NN+ FN H A +GN + L+R ++V+S+K+D F ALHLA NNH+ + +L+K + D+N+RNN QT LH+AV+Q +++ + +LV +GA+++ D +GDTP+HI + ++ + + G S + + AS G+ + +A +L A + N K+P+D L + A R + I I T + A P + + QH L+ D++ +EEL+ R + ME+ TC IC D I + F CGH +C C+ +L C ICR+ I +I+++
Sbjct: 222 DGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTK-------------------------QNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKL----LSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNP-NCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFRYQRAILPYIHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQGGLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIF 951 HSP 2 Score: 65.4698 bits (158), Expect = 4.588e-10 Identity = 39/144 (27.08%), Postives = 74/144 (51.39%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQYD------------TRQD-HKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D+E ++ Q HGGWN M E + G +G V+R+ ++ +QY+ T+Q+ +G V +++N + K+LQV +GEW+DDM + LG+ ++I+ F DG++++ G+++ N + +
Sbjct: 285 DVEILREMQEGHGGWNPKMAEFI-----------------GQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTS 411
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 244.588 bits (623), Expect = 3.452e-67 Identity = 193/719 (26.84%), Postives = 338/719 (47.01%), Query Frame = 2
Query: 686 DGDMYVSRLGMEWMINPKCI----------------GKRNE--NKFKSEK--LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVD-RLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEAT----DSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDA-GADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGS---DKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVT-FGCGH-VACDVCANNLYECHICRESIENKIR 2740
DG V R+G + ++ KCI GK E K SE+ + GD V+ D++ ++++Q+ HGGW M G+ V I GDV+V + ++P LTK F GD VR+++N +K+LQ GHGEW + M LG++GKV V +GD++V + GQ NP C+ + D++ + + + N L I+ + S + D + + GN +VR+++ + +G L +A + G+++VVK+L++ ANI+ +++ GDTALH A ++ + +L++KGA+ NN K T + +A + G ++ ++L N +++ +S DT LH A+ + L + D NN+ FN H A +GN + L+R ++V+S+K+D F ALHLA NNH+ + +L+K + D+N+RNN QT LH+AV+Q +++ + +LV +GA+++ D +GDTP+HI + ++ + + G S + + AS G+ + +A +L A + N K+P+D +++ L + I+ PT S + + T + L E C++C + + V+ F C H + C+ C+ + +C C+ I K+R
Sbjct: 205 DGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTK-------------------------QNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKL----LSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNP-NCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIVFPVQDLLCF--------HVSHAIISLDSPTVSC---------------SLRRVHTTPNTMTNLSPCSAASPAE--CLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLR 867 HSP 2 Score: 158.303 bits (399), Expect = 6.229e-39 Identity = 186/765 (24.31%), Postives = 316/765 (41.31%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLG-MEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQ-ANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAM--QFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLL---EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLIN-KGADVNAQNNNKITPIMLAASAGS-MDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAG-ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNH--------EGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPL-------------HIAVRIHSMDTVKKRGKLLGI-------SGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
KV + + E+++ +Q+ HGGW M FIG+ + +I + GD+ V W +P + K+N GD+V++ D++ +K+LQ HG W + M G+I KV + GD++V+V +NP LT + + AN + + L + K+ S QK E ++ + G KV+ + KV I Q T S +V L Q D D DE D T + GN V +++ S G + L L IAVN G +VV++L IN ++ GDT LH A+ D I ++L D QNN + +A G+ + ++++L +R + + TALHLA N H E V L+ G D+ NN+N HL +G+ ++ + + EG VN++ +D +H+ H EG LL K + + N++ + A++ C L GA+I+ + G +PL H++ I S+D+ L + + + +A+S +LA+ + + I C+ +RRM K I+ ++ A+ + ++ + + + + + +EEL+ R + ME+ TC IC D I + F CGH +C C+ +L C ICR+ I +I+++
Sbjct: 262 KVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQN-------SFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLS-----------------QKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQG---------RTALQVASHLGYMEVVKVLLQANANIDLRD--DEGD-----TALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQL-VDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVT-EGAD-VNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLT-KLEASGLPGNIELNVGSAMA-----CF--LALSGADINYANHRGKSPLDLIVFPVQDLLCFHVSHAIISLDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSI----------VCEEC---------SRRMKKCIKCQVCIARK---------LRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIF 947 HSP 3 Score: 65.4698 bits (158), Expect = 3.578e-10 Identity = 39/144 (27.08%), Postives = 74/144 (51.39%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQYD------------TRQD-HKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D+E ++ Q HGGWN M E + G +G V+R+ ++ +QY+ T+Q+ +G V +++N + K+LQV +GEW+DDM + LG+ ++I+ F DG++++ G+++ N + +
Sbjct: 268 DVEILREMQEGHGGWNPKMAEFI-----------------GQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTS 394
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Xenopus
Match: coa7 (cytochrome c oxidase assembly factor 7 (putative) [Source:Xenbase;Acc:XB-GENE-6258829]) HSP 1 Score: 244.202 bits (622), Expect = 3.917e-67 Identity = 194/719 (26.98%), Postives = 339/719 (47.15%), Query Frame = 2
Query: 686 DGDMYVSRLGMEWMINPKCI----------------GKRNE--NKFKSEK--LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVD-RLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEAT----DSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLL--EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDA-GADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGS---DKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVT-FGCGH-VACDVCANNLYECHICRESIENKIR 2740
DG V R+G + ++ KCI GK E K SE+ + GD V+ D++ ++++Q+ HGGW M G+ V I GDV+V + ++P LTK F GD VR+++N +K+LQ GHGEW + M LG++GKV V +GD++V + GQ NP C+ + D++ + + + N L I+ + S + D + + GN +VR+++ + +G L +A + G+++VVK+L++ ANI+ +++ GDTALH A ++ + +L++KGA+ NN K T + +A + G ++ ++L N +++ +S DT LH A+ + L + D NN+ FN H A +GN + L+R ++V+S+K+D F ALHLA NNH+ + +L+K + D+N+RNN QT LH+AV+Q +++ + +LV +GA+++ D +GDTP+HI + ++ + + G S + + AS G+ + +A +L A + N K+P+D L A++ +++E PT S + + T + L E C++C + + V+ F C H + C+ C+ + +C C+ I K+R
Sbjct: 205 DGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTK-------------------------QNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKL----LSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNP-NCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILER-ARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVEL-----DSPTVSC---------------SLRRVHTTPNTMTNLSPCSAASPAE--CLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLR 870 HSP 2 Score: 159.458 bits (402), Expect = 2.378e-39 Identity = 184/762 (24.15%), Postives = 313/762 (41.08%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLG-MEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQ-ANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAM--QFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLL---EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLIN-KGADVNAQNNNKITPIMLAASAGS-MDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAG-ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNH--------EGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPS---LKEKLSV--------------ANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
KV + + E+++ +Q+ HGGW M FIG+ + +I + GD+ V W +P + K+N GD+V++ D++ +K+LQ HG W + M G+I KV + GD++V+V +NP LT + + AN + + L + K+ S QK E ++ + G KV+ + KV I Q T S +V L Q D D DE D T + GN V +++ S G + L L IAVN G +VV++L IN ++ GDT LH A+ D I ++L D QNN + +A G+ + ++++L +R + + TALHLA N H E V L+ G D+ NN+N HL +G+ ++ + + EG VN++ +D +H+ H EG LL K + + N++ + A++ C L GA+I+ + G +PL + G++ + F D P + ++ T + +P P+ + +L+V +RRM K I+ ++ A+ + ++ + + + + + +EEL+ R + ME+ TC IC D I + F CGH +C C+ +L C ICR+ I +I+++
Sbjct: 262 KVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQN-------SFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLS-----------------QKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQG---------RTALQVASHLGYMEVVKVLLQANANIDLRD--DEGD-----TALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQL-VDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVT-EGAD-VNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLT-KLEASGLPGNIELNVGSAMA-----CF--LALSGADINYANHRGKSPLDLIA----------DGRITHLVKNFAQKFREQVELDSP---TVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARK---------LRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIF 950 HSP 3 Score: 65.4698 bits (158), Expect = 4.358e-10 Identity = 39/144 (27.08%), Postives = 74/144 (51.39%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQYD------------TRQD-HKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D+E ++ Q HGGWN M E + G +G V+R+ ++ +QY+ T+Q+ +G V +++N + K+LQV +GEW+DDM + LG+ ++I+ F DG++++ G+++ N + +
Sbjct: 268 DVEILREMQEGHGGWNPKMAEFI-----------------GQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTS 394
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 283.108 bits (723), Expect = 1.801e-80 Identity = 208/736 (28.26%), Postives = 361/736 (49.05%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL+++ ++ A++++GD A+H A D+ + ++L AD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV GA++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DPSL + L+ + ++S ++ ++ S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANIVRSG-DAAQGAEGGTSQ--------FQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKV------APAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSR--------------------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 57.7658 bits (138), Expect = 7.336e-8 Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQY------DTRQ-------DHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + D Q ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397 HSP 3 Score: 51.6026 bits (122), Expect = 5.093e-6 Identity = 40/176 (22.73%), Postives = 75/176 (42.61%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXK------VMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGK 751
G+ YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + + V + + E +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSK 399
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 283.108 bits (723), Expect = 1.801e-80 Identity = 208/736 (28.26%), Postives = 361/736 (49.05%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL+++ ++ A++++GD A+H A D+ + ++L AD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV GA++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DPSL + L+ + ++S ++ ++ S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANIVRSG-DAAQGAEGGTSQ--------FQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKV------APAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSR--------------------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 57.7658 bits (138), Expect = 7.336e-8 Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQY------DTRQ-------DHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + D Q ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397 HSP 3 Score: 51.6026 bits (122), Expect = 5.093e-6 Identity = 40/176 (22.73%), Postives = 75/176 (42.61%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXK------VMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGK 751
G+ YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + + V + + E +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K
Sbjct: 224 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSK 399
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Mouse
Match: Mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:MGI Symbol;Acc:MGI:2443157]) HSP 1 Score: 223.402 bits (568), Expect = 7.106e-62 Identity = 155/547 (28.34%), Postives = 277/547 (50.64%), Query Frame = 2
Query: 1169 GKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
GKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL+++ ++ A++++GD A+H A D+ + ++L AD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV GA++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DPSL + L+ + ++S ++ ++ S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 2 GKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKV------APAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSR--------------------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 498 HSP 2 Score: 130.568 bits (327), Expect = 8.742e-31 Identity = 179/682 (26.25%), Postives = 289/682 (42.38%), Query Frame = 2
Query: 884 GKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALL---DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL------KYKADINIRNNNLQTALHIAVSQK----YLNCMKILVEKGAEIDVTDANGDTPL----------------------HIAVRIHSM-----DTVK--------KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNK---NDKTPMDFCDPSLKEKLSVANARRMSKEIETK-ILQAQGPTTVAAGGLMAH-PGDHIDRNSAQHL-KTKD-----EELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
GK+ +VQ I D+KV V YNP ++K + + A G+R L +L K+F + L N+ +K G V ++ +L K VDVNG +C T S+ V+ L ++ Q+ E+++ D DR G + EV + + PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + I +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ +A DI+ VN + A HL +R + +I+ + + R G K+ + Q D LH A+ ++ LR L K A N L L ++K + C L GA++ + + G +PL + R SM +T++ KR L G G A+ + S + + I + ++RT I+ +DK P A M K ++ + +++ + P GG + P D I + L K KD ++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 2 GKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSAISNASGER-----LSQLLKKLFETQESGDL--NEELVKAAANGD---VAKVEDLL----KRPDVDVNG---------------QCAGHTAMQAASQ---NGHVDILKLLLKQNVDV-EAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQT--ALHLAVERQHTQIVRLLV-RAGAKL-DIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACF--LAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCG---HIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCCGGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY 641
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Mouse
Match: Mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:2679684]) HSP 1 Score: 204.912 bits (520), Expect = 4.991e-54 Identity = 149/577 (25.82%), Postives = 269/577 (46.62%), Query Frame = 2
Query: 1067 FSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN----LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVK-NFHAESVNALLD--AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHS-MDTVKKRGKLLGISGIFQSTASSNEGSDKP--------EDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
F GD VR+I + +KRLQ GHGEW + M LG+VGKV V +G+L+V + GQ+ +P C+ A + ++ D+ + + + + + D + +GN L+ +R+ + V T +G L +A G +++V+LL++ RA+++ ++ G+T LH ++ + +L++ G V+A+N + T + +A G +++ ++L R D+++ ++ DT LH A+ A S+ +L G D+ A N++ F H + +G+ + L R ++V+++K+D F ALHLA NNH +VL++ + D+N+RN LQ+ LH+AV Q +L + +LV+ G ++ D GDT LH+A++ H + V R G G Q S + S P +A +L + A N ++P+D K A+R + +H+ + + L + C++C + + + F C H C+ CA + +C C+ I K+R
Sbjct: 292 FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKN--QGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILC-ERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILAR-ARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAG--GDPGPLQ-LLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVP--------------------PGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLR 841 HSP 2 Score: 52.373 bits (124), Expect = 3.394e-6 Identity = 29/129 (22.48%), Postives = 51/129 (39.53%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D + ++ Q HGGWN M E +G V +I + + KRLQ +GEW+DDM LG+ ++++ F DGN+++ G+ + + ++
Sbjct: 268 DTDVLRDMQEGHGGWNPRMAE--------------------------------------HNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVA 358
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Mouse
Match: Mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:MGI Symbol;Acc:MGI:2679684]) HSP 1 Score: 128.257 bits (321), Expect = 1.845e-31 Identity = 91/373 (24.40%), Postives = 169/373 (45.31%), Query Frame = 2
Query: 686 DGDMYVSRLGMEWMINPKCIGKRNENKFKSEKL--------------------RKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDR-LELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGN----LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLL 1729
DG V R+G + ++ +C+G+ + E L ++GD V+ D ++ +Q+ HGGW M G++ V I GDV+V + ++P LTK F GD VR+I + +KRLQ GHGEW + M LG+VGKV V +G+L+V + GQ+ +P C+ A + ++ D+ + + + + + D + +GN L+ +R+ + V T +G L +A G +++V+LL++ RA+++ ++ G+T LH ++ + +L++ G V+A+N + T + +A G +++ ++L
Sbjct: 30 DGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNS-------------------------FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKN--QGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKIL 375 HSP 2 Score: 79.7221 bits (195), Expect = 2.823e-15 Identity = 82/333 (24.62%), Postives = 145/333 (43.54%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVS-RLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLT--KCEGNQANPTAARGQRRRSS---ALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQ--LTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEG---VYPLMIAVNGGHIDVVKLL 1531
KV + +++V++++Q+ HGGW M FIG+ + +I GD+ V W +P + K N GD+V++ D+ +K+LQ HG W + M G++ KV + G+++V V ++P L + E + A R + +SS AL+KL + P RL+ ++ LG V +++D+ L +PE V+ T + + + + Q L +++ Q+ S D PD D T + +GN E +++ S G + L +AV G ++VVK+L
Sbjct: 87 KVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNS-------FWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLV-------------------VEAALGNV--ARALDL------------LRRHPEQVD-TKNQGRTALQVAAYLGQVELVRLLLQARAS--MDLPD-DEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKIL 375 HSP 3 Score: 66.2402 bits (160), Expect = 6.713e-11 Identity = 75/332 (22.59%), Postives = 138/332 (41.57%), Query Frame = 2
Query: 1025 RRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQ-KLNLNPECVEATDSDCESEPDIQTMVNQLTQIMG-QSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHID-----VVKLLIEKRANINAKEENGDTALHIAVQKDKFKI-----------------------CDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMF 1930
+RR SA + + F GDKV+ + + ++ +Q+GHG W M +G++G V + GD++V+ + + +P + +S D+ ++ L + Q+ H + +D+ A +G + +V K+ G L +AV G +V E+ AN++ E + ++V DK + DLL V+ +N + T + +AA G +++ RLL +R A + + + E +T LH E+ LL AG + A N A H+ QRG ++++
Sbjct: 72 QRRVSA----DGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVG--DVVRVIGDLDTVKRLQAGHGEWTDD----------MAPALGRVGKVVKVF----------GDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGR-TALQVAAYLGQVELVRLLLQAR-ASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKIL 375 HSP 4 Score: 58.5362 bits (140), Expect = 2.138e-8 Identity = 36/144 (25.00%), Postives = 66/144 (45.83%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLN--SELLIQY--DTRQDHK-----------IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT 511
D + ++ Q HGGWN M E + G G V+R+ ++ +Q+ +TR +G V +I + + KRLQ +GEW+DDM LG+ ++++ F DGN+++ G+ + + ++
Sbjct: 93 DTDVLRDMQEGHGGWNPRMAEFI-----------------GQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVA 219
BLAST of E3 ubiquitin-protein ligase MIB2 vs. UniProt/SwissProt
Match: sp|Q80SY4|MIB1_MOUSE (E3 ubiquitin-protein ligase MIB1 OS=Mus musculus OX=10090 GN=Mib1 PE=1 SV=1) HSP 1 Score: 283.108 bits (723), Expect = 1.260e-79 Identity = 208/736 (28.26%), Postives = 361/736 (49.05%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL+++ ++ A++++GD A+H A D+ + ++L AD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV GA++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DPSL + L+ + ++S ++ ++ S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANIVRSG-DAAQGAEGGTSQ--------FQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKV------APAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSR--------------------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 57.7658 bits (138), Expect = 5.132e-7 Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQY------DTRQ-------DHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + D Q ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of E3 ubiquitin-protein ligase MIB2 vs. UniProt/SwissProt
Match: sp|Q86YT6|MIB1_HUMAN (E3 ubiquitin-protein ligase MIB1 OS=Homo sapiens OX=9606 GN=MIB1 PE=1 SV=1) HSP 1 Score: 281.952 bits (720), Expect = 3.101e-79 Identity = 206/732 (28.14%), Postives = 361/732 (49.32%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASS---------NEGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V S + + +L+Q++ + + ES + + + K N G++ +V L+ V + G + A GH+D++KLL+++ ++ A++++GD A+H A D+ + ++L AD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV GA++D+ D +GDTPLH A+R H++ +++ + + + + S +G++K +A +L A I+NK ++P+D C DP+L + L+ + ++S ++ ++ S + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANIVRSG-DAAQGAEGGTSQ--------FQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASG--ERLSQLLKKLFETQESGDLNEELVKAAAN----GDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSR--------------------------SPSMISNDSETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 863 HSP 2 Score: 57.7658 bits (138), Expect = 5.220e-7 Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQY------DTRQ-------DHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + D Q ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of E3 ubiquitin-protein ligase MIB2 vs. UniProt/SwissProt
Match: sp|Q804S5|MIB1_DANRE (E3 ubiquitin-protein ligase mib1 OS=Danio rerio OX=7955 GN=mib1 PE=1 SV=1) HSP 1 Score: 281.182 bits (718), Expect = 6.724e-79 Identity = 213/741 (28.74%), Postives = 366/741 (49.39%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK---------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++++N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ L+ + + P HI S + K + ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGSSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV------APAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 905 HSP 2 Score: 171.4 bits (433), Expect = 3.320e-42 Identity = 185/739 (25.03%), Postives = 309/739 (41.81%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEG---VYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALLDAG---ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + T A G+R L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S+ V+ L ++ HS + + D D + +A G+ V +++ G PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ G D++ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + M LG G + +A+S +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ + L + +E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 259 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGER-----LSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAASQ---NGHVDVLKLLL---KHSVDLEAEDKDGDRAVHHA-SFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTLIMG--------LGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSR---------------------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 861 HSP 3 Score: 67.0106 bits (162), Expect = 7.474e-10 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 970 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1030 HSP 4 Score: 56.225 bits (134), Expect = 1.727e-6 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQDHK-------------IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + + +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397
BLAST of E3 ubiquitin-protein ligase MIB2 vs. UniProt/SwissProt
Match: sp|Q6GNY1|MIB1_XENLA (E3 ubiquitin-protein ligase mib1 OS=Xenopus laevis OX=8355 GN=mib1 PE=2 SV=1) HSP 1 Score: 274.248 bits (700), Expect = 1.646e-76 Identity = 204/731 (27.91%), Postives = 360/731 (49.25%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN----------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
+ +G + R+G E M + KC+ G++N N+ L GD+V I +++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA+G +S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V S + + +L+Q++ + + ES + + + K N + ++++ K D V + G + A GH+D++KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L + S+ +SE DT LH A+ + + LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV A++D+ D +GDTPLH A+R H++ +++ + + + +T + +G++K +A +L A ++NK ++P+D C DP+L + L+ + + S ++ G H S + E LEE C++C D + FG CGH+A C +C+ + +C +C+E ++++ ++
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLLIGDLVNIDLELEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSG-DAAQGAEGGTSP--------FQVGDLVQICYDIERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATG--ERLSQLLKKLFETQESGDLNEELVKAAANG-DVAKVDDLLKRQD-VDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEGTVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKKL-LDFSCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTTWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLAVRNKKGQSPLDLCPDPNLCKTLAKCHKEKSSGQV----------------------GSH----SPSMINNDSETLEE-------------CMVCSDLKRDTLFGPCGHIATCSLCSPRVKKCLLCKEQVQSRTKI 864 HSP 2 Score: 160.999 bits (406), Expect = 5.218e-39 Identity = 197/800 (24.62%), Postives = 321/800 (40.12%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEG---VYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKI------------------------------------TPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNS----------------QKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK-----KRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNK---NDKTPMDFCDPSLKEKLSVANARRMSKEIETK-ILQAQGPTTVAAGGLMAHP-GDHIDRNSAQHL-KTKD-----EELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + + + GD+VQIC DI++IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +++ + + A G+R L +L K+F + L N+ +K G V+ + K + VDVNG +C T S+ ++ L + HS + + D D + +A G+ V +++ G PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + I +L+ GADV NNN T + LAA +++ LL +A+L I N TALHLAV+ H + V L+ A A + + H + L + V V++ Q +K +A +A F L AD+ +RN Q+ L + N K L + E +P I +++ KR L G G A+ + S + + + + ++RT I+ +DK P A M K ++ + +++ + P + G D I + L K KD ++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 259 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDIERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAVSRVASVGSAISNATGER-----LSQLLKKLFETQESGDL--NEELVKAAANGDVAKVDDLL-------KRQDVDVNG---------------QCAGHTAMQAASQNGHVDILKLLLK------HSVDVEAEDKDGDRAVHHA-AFGDEGTVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLLDFSCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTTTWEPSKNTLIMGLGTQGAEKKSAASIACF---------LAANGADLAVRNKKGQSPLDLCPDP---NLCKTLAKCHKEKSSGQVGSHSPSMINNDSETLEECMVCSDLKRDTLFGPCG---HIATCSLCSPRVKKCLLCKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFVMCCSGKGTEDVADDIAGGNIPALQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1007 HSP 3 Score: 56.225 bits (134), Expect = 1.619e-6 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQD-------------HKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
++E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + ++G V + + E K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 ELEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSPFQVGDLVQICYDIERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVCGTSWTYNPAAV 397
BLAST of E3 ubiquitin-protein ligase MIB2 vs. UniProt/SwissProt
Match: sp|Q9VUX2|MIB_DROME (E3 ubiquitin-protein ligase mind-bomb OS=Drosophila melanogaster OX=7227 GN=mib1 PE=1 SV=3) HSP 1 Score: 271.166 bits (692), Expect = 1.278e-74 Identity = 201/699 (28.76%), Postives = 336/699 (48.07%), Query Frame = 2
Query: 791 GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRL-ELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECV----EATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDS----------------VGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVK-IVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK-----------------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKT-----CIICYDRPINVTF-GCGHVA-CDVCANNLYECHICRESIENKIRL 2743
GD V + D++ ++ LQ HGGW + M C V I D+ V + +NP +LTK ++PT A + F GD V++ ++ IK LQ+GHGEW +AMQ LGK+G+V+ V + DLKV++ NP V +T SD P I + +L+ I+ + + S D T+ F A G + + V + + G L A GHI+V+++L+ ++ ++++GD A+H A D+ + ++L GAD+NA+N + T + +A + G +++ + L T S+ +SE DT LH A+ H E ++ LLD GADI NN FNA H A +GN +++ L + IV +KDD + ALHLA NNH +L+ KA+++ +N NLQTALH+AV ++++ +K+LV+ GA++++ D +GDTPLH A+R H++ +K+ + G + ++N +A +L A +KN+ +TP+D C DP+L + L R + + E L + L A S+ + + E+ Q + + + + C++C D + F CGHV+ C+ CA + +C ICRE++ ++ ++
Sbjct: 343 GDKVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKV----SSPTTAPPE--------------FQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSS-ERLSAILKKLFEPNVS----GDATEEFVKAAANGFAARCEEYLAGAAQPSTSSASPSSGPDVNVNGVFAGHTALQAASQNGHIEVIQVLLRHAVDVEIEDKDGDRAVHHAAFGDEAAVIEILAKAGADLNARNKRRQTSLHIAVNKGHLNVVKTLLT-LGCHPSLQDSEGDTPLHDAISKEHDEMLSLLLDFGADITLNNNNGFNALHHAALKGNPSAMKILLTKTNRPWIVEEKKDDGYTALHLAALNNHVEIAELLVHMGKANMDRQNVNLQTALHLAVERQHVQIVKLLVQDGADLNIPDKDGDTPLHEALRHHTLSQLKQLQDVEGFGKLLMGLRNANN-KKASASIACFLAANGADLTLKNRKQQTPLDLCPDPNLCKTLVKCYNERKTDDSELPGNVAGTSSSARARAASGSLNQSSSVNMPLSSLAA--SSTFPAASSSSIFALNGIANEMSQSLHEDPPKSSASLDECLVCSDAKRDTVFKPCGHVSCCETCAPRVKKCLICRETVSSREKI 1014 HSP 2 Score: 96.6709 bits (239), Expect = 6.275e-19 Identity = 54/171 (31.58%), Postives = 87/171 (50.88%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSR-LGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSP 1075
KV + + E++++LQ HGGW + M + +V I +D D+ V+ G W NP + K + + + GDIV+IC D++ IK LQ+ HG W ++M+ GKI +VQ + D+KV V YNP+ + K + A+ + S L + K+F P
Sbjct: 345 KVTVDLDLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAVCKVASSTASDGSCAPVIPSSERLSAILKKLFEP 515 HSP 3 Score: 65.0846 bits (157), Expect = 3.347e-9 Identity = 22/62 (35.48%), Postives = 45/62 (72.58%), Query Frame = 2
Query: 2561 EELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
+++++L+Q++QD++++ C +C+DR N+ F CGH C +C + + C ICR+++E +I L+
Sbjct: 1165 DDVQKLKQQLQDIKEQTMCPVCFDRIKNMVFLCGHGTCQMCGDQIEGCPICRKTVEKRILLF 1226 HSP 4 Score: 60.077 bits (144), Expect = 1.163e-7 Identity = 40/141 (28.37%), Postives = 66/141 (46.81%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQ-SLGSCAIVKGPSGNVYRLNSELLIQYDTR----QDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTS 532
D+E ++ Q HGGW M ECL+ +V +V SGN + N +L + + + ++G V + + E K LQ +GEW+D M LGK + Q + D ++K++ ++ N A+ CK S
Sbjct: 351 DLEIVQSLQHGHGGWTDGMFECLSNAGMVVGIDEDHDIVVAYNSGNRWTFNPAVLTKVSSPTTAPPEFQVGDIVKICSDVESIKILQRGHGEWADAMQLTLGKIGRVQQVYHDNDLKVEVGNTSWTYNPLAV----CKVAS 487
BLAST of E3 ubiquitin-protein ligase MIB2 vs. TrEMBL
Match: A0A0L8FX87 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22005289mg PE=4 SV=1) HSP 1 Score: 310.071 bits (793), Expect = 1.161e-88 Identity = 242/790 (30.63%), Postives = 393/790 (49.75%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GME---WMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCE-----GNQANPTAARGQRRR--------SSALEKLEVKV---------FSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECV-EATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL-DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPED----LAIYLLKKKARTDIKNKNDKTPMD-FCDPSLKEKL----SVANARRMSKEIETK-------ILQAQGPTTVAAGGLMAHPGDHI-------DRNSAQH-------LKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
+V + + +K LQ+ HGGW M FIG+ V IV+ + + D + + G+ W NPK + K +K GD V+I DDI+++K+ Q NHGGW M GKI V+ P GD+ V+V ++NP+ TK G + + T+ + S L+ ++ + F G+ V++I + K+K Q+ HG WV+ M +LGK +VK V NG L V + G+ ++NP+C + + S +SE D +++ + + Q+ S + D F G+ VR+ + D + G L +A GH D+V +L+E A++ ++++GDTAL+ + + +I +LL++KGA NA N N+ T + +A + S+ R+L +A ++I +S DTALH A++ + E ++ L+ D+ D + N + FN H A RGN L++ IVN +KDD F ALHLA N H LL +A+INI+N++ QT L +A +Q ++ +++L+ KGAE++V D +G+ LH+AV ++ K K++ S G D+ E +A YL+ K A KN+ D P+D D SL EKL S++ + +IE + +L + TV + P H+ R +H + K + ++RI+++E+ TC IC DRP N+ F CGH C C +L ECH+C + I+ K+ LY
Sbjct: 15 RVSMYEDINTIKLLQEGHGGWNPFMANFIGQ-VGIVQGVNNVDDVIVKFEGLSKNLWRFNPKALTK-------IDKFSVGDAVKILDDIEQVKRYQINHGGWSPDMTSVLGKIGSVKIAFPDGDLFVSVSGKIFLFNPLCCTKVPQSMKVGEEDDATSIMEVMEQFFNLKTGFSGLLQSMQGIMGDSERQDDQFLVGETVKIIEDIQKVKAYQEKHGGWVDDMVSILGKTARVKLVLPNGALLVSVSGKMCSINPKCCRKVSRSVDDSESDSESVDDMMQQLFNMSFGATVLD--------GFVKEAAKGHTRNVREFIKNHRDEIDKKC--SGKTALQVASYKGHKDIVLMLLEAGADLELQDDDGDTALNFSAIGGETEIMELLLSKGAKTNALNKNRHTALHIAVNKESVKCVRIL-VKHSAPINIQDSLGDTALHDAIRKENEEIIDMLISDSRIDFKIRNKRGFNTLHYAAIRGNNFATVSILQK-CQDIVNVKKDDGFTALHLAALNGHREVANTLLSVGRAEINIKNSSGQTPLFLAATQGHIGLIELLILKGAEVNVEDEDGNICLHLAVSRQNVSISKDDLKII-------ENIRSQLGLDESERAEVVIACYLVHKGANPYYKNRQDVMPLDIMSDQSLIEKLVKVASLSTEQPCGAQIEERRSNNKDCMLCSNKQATV-----LFQPCCHVLICEHCNVRVPVKHCLQCKVLIADKITIHDPQQKRIEELEERVTCPICMDRPRNMAFECGHTVCKECGESLKECHMCSKPIQKKLNLY 772 HSP 2 Score: 129.413 bits (324), Expect = 7.520e-27 Identity = 153/611 (25.04%), Postives = 263/611 (43.04%), Query Frame = 2
Query: 317 IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQD-GNVKIKYRGRT---FRINSKAILTDNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCI----------------------------------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVG--TSTLLEGVY-PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKG-ADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAG-ADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAV 2002
+G V++ E+ K LQ +G W+ M N +G+ V I+Q + +V +K+ G + +R N KA + + F G+ V ++ + E +K Q +HGGW M +G+ + DGD++VS G ++ NP C G +++ + ++ G+ V+I +DIQK+K Q+ HGGWV+ M GK +V+ +LP+G + V+V NP K + S +++ + ++F+ +++ +K KGH N +F I + ++ +C T S + +V L + +D + D+ D T N IG E+ +L+ S G T+ L + + L IAVN + V++L++ A IN ++ GDTALH A++K+ +I D+LI+ D +N + AA G+ T + +++ + TALHLA N H E N LL G A+I N+ L A +G+ ++E+ + + G + VN + +D LHLAV
Sbjct: 12 VGDRVSMYEDINTIKLLQEGHGGWNPFMANFIGQ-VGIVQGVNNVDDVIVKFEGLSKNLWRFNPKA----------LTKIDKFSVGDAVKILDDIEQVKRYQINHGGWSPDMTSVLGKIGSVKIAFPDGDLFVSVSGKIFLFNPLCCTKVPQSMKVGEEDDATSIMEVMEQFFNLKTGFSGLLQSMQGIMGDSERQDDQFLVGETVKIIEDIQKVKAYQEKHGGWVDDMVSILGKTARVKLVLPNGALLVSVSGKMCSINPKCCRKVS------RSVDDSESDSESVDDMMQQLFNMSFGATVLDG--FVKEAAKGHTR--NVREF--------------------IKNHRDEIDKKCSGKTALQVASYKGHKDIVLMLLE--AGADLELQDDDGD-----TALNFSAIGGETEIMELLLSKGAKTNALNKNRHTALHIAVNKESVKCVRILVKHSAPINIQDSLGDTALHDAIRKENEEIIDMLISDSRIDFKIRNKRGFNTLHYAAIRGNNFATVSILQKCQDIVNVKKDDGFTALHLAALNGHREVANTLLSVGRAEINIKNSSGQTPLFLAATQGHIGLIELLILK-GAE-VNVEDEDGNICLHLAV 572 HSP 3 Score: 116.701 bits (291), Expect = 7.171e-23 Identity = 150/572 (26.22%), Postives = 245/572 (42.83%), Query Frame = 2
Query: 791 GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLE---LVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAV---------------NGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKI---CDLLINKGADVNAQNNNKITPIMLAASAGSMDITRL--------LATSRNADLSIGNSEND--------TALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILV-EKGAEIDVTDANGDTPLHI-AVRIHSMDTVKKRGKLLGISGIFQS---TASSNEGSDKPEDLAIYLLK-KKARTDIKNKNDKTPM 2377
GD V + +DI IK LQ+ HGGW M G++ VQ + DV V + L +NP LTK + FS GD V+++++ ++KR Q HG W M VLGK+G VK +GDL V + G+ NP C C P Q+M +G+ D + E + FFN LK G ++ + +G S + + + V +GG +D + ++ K A + NG AL ++V I C +++ D + ++ + +M S T L +RN I N ++ TAL +A H + V LL+AGAD+ ++ A + A G I+E+ L + G K N+ ++ ALH+AV C+R+L+K+ A INI+++ TALH A+ ++ + +L+ + + + + G LH A+R ++ TV K I + + TA + ++A LL +A +IKN + +TP+
Sbjct: 13 GDRVSMYEDINTIKLLQEGHGGWNPFMANFIGQVGIVQGVNNVDDVIVKFEGLSKNLWRFNPKALTK-------------------------IDKFSVGDAVKILDDIEQVKRYQINHGGWSPDMTSVLGKIGSVKIAFPDGDLFVSVSGKIFLFNPLC-------CTKVP--QSMK------VGEEDDATSIMEV----MEQFFN-LKTGFSGLLQSMQGIMGDSERQDDQFLVGETVKIIEDIQKVKAYQEKHGGWVDDMVSILGKTARVKLVLPNG--ALLVSVSGKMCSINPKCCRKVSRSVDDSESDSESVDDMMQQLFNMSFGATVLDGFVKEAAKGHTRNVREFIKNHRDEIDKKCSGKTALQVASYKGHKDIVLMLLEAGADLELQDDDGDTALNFSAIGGETEIMELLLSK-GAK-TNALNKNRHTALHIAVNKESVKCVRILVKHSAPINIQDSLGDTALHDAIRKENEEIIDMLISDSRIDFKIRNKRGFNTLHYAAIRGNNFATVSILQKCQDIVNVKKDDGFTALHLAALNGHREVANTLLSVGRAEINIKNSSGQTPL 535 HSP 4 Score: 81.2629 bits (199), Expect = 8.810e-12 Identity = 71/261 (27.20%), Postives = 117/261 (44.83%), Query Frame = 2
Query: 122 KDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSC--AIVK--GPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVM-------------------LIRNSE-------------VMKNLQK------DHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSE 778
+D+ TIK+ Q HGGWN M G +VQ + + IVK G S N++R N + L + D +G V ++++ E+ KR Q+ +G WS DM ++LGK + F DG++ + G+ F N T + + E+ +VM ++ +SE +++++QK HGGW + M +G+ + +L +G + VS G INPKC K + + SE
Sbjct: 20 EDINTIKLLQEGHGGWNPFMAN-FIGQVGIVQGVNNVDDVIVKFEGLSKNLWRFNPKALTKIDK---FSVGDAVKILDDIEQVKRYQINHGGWSPDMTSVLGKIGSVKIAFPDGDLFVSVSGKIFLFNP-LCCTKVPQSMKVGEEDDATSIMEVMEQFFNLKTGFSGLLQSMQGIMGDSERQDDQFLVGETVKIIEDIQKVKAYQEKHGGWVDDMVSILGKTARVKLVLPNGALLVSVSGKMCSINPKCCRKVSRSVDDSE 275
BLAST of E3 ubiquitin-protein ligase MIB2 vs. TrEMBL
Match: A0A498NZI9 (E3 ubiquitin-ligase mib1 isoform X1 OS=Labeo rohita OX=84645 GN=ROHU_002127 PE=4 SV=1) HSP 1 Score: 305.064 bits (780), Expect = 7.397e-84 Identity = 242/883 (27.41%), Postives = 419/883 (47.45%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVM------LIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVE-----------ATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK---------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
G YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + M + + + +K LQ+ HG W E M +G+ + +I D D+ V G RN L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V A+ + P + +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N K TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A+++I+N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ L+ + + P HI S + K + ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 214 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMYETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGCSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEN------------GNRNPGG-----LQIGDLVNIDLDLEIVQSLQHGHGGWTDGMYETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGCSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVP--AERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRKQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSANLDIQNANQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGAELTIRNKKGQSPLDLCPDPSLCKALTKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 1053 HSP 2 Score: 118.242 bits (295), Expect = 4.681e-23 Identity = 142/582 (24.40%), Postives = 240/582 (41.24%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVK---------IMGQKLN--LNPECVEATDSDCESEPD--IQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGT---------STLLEGVYPL--MIAVNGGHIDVVKLLIEKRANINAKEE-NGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIM-----LAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFA--ALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGI 2251
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV+ +G +N L+ E V++ D +T+ T DH P +R FN + N VR + G L++ Y + + + GH + + ++ + ++ D+ L + V + + K A + N ++ + A S + +L T + D+ N+ + A A+ + L D+ A +Q G+ +L++ LK V+ + +DK A+H A F + + VL + AD+N RN QT LHIAV++ +L +K L++ G + D+ GDTPLH A+ KKR +L +
Sbjct: 186 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMYETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGCSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVENGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMYETLTTTGTVCGIDEDHDIVVQYPSGNRWT--FNPAVLTKANVVRSGEVAAGAEGGCSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDI------NEELVKAAANGDLAKVEDILKRPDVDVNG-QCAGHTAMQAASQNGHVDVLKLLLKHN----VDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRKQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAIS-------KKRDDMLSV 729 HSP 3 Score: 67.0106 bits (162), Expect = 2.188e-7 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 1165 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1225
BLAST of E3 ubiquitin-protein ligase MIB2 vs. TrEMBL
Match: A0A1S3II44 (E3 ubiquitin-protein ligase MIB2 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106164494 PE=4 SV=1) HSP 1 Score: 297.745 bits (761), Expect = 6.060e-83 Identity = 223/705 (31.63%), Postives = 343/705 (48.65%), Query Frame = 2
Query: 779 KLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCE------SEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNL-NEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLD-AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMD-FCDPSLKEKL-------------SVANARRMSKEIE------------------------TKILQAQGP--TTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
+ R GD V++ D+ +K +Q+ HGGW M GK+ V I GDV+V EG Q T G AL K V+ FS D V++ N H++++LQ GHGEW ++M+ LGK+GKV V +GDL+V + Q NP C E + + + LT ++ Q D DR I N+ N V K D V + G L +A + GHI++VKLL+ AN+ ++E+GDTALH A ++ +I + L++KGA++NA NN + + +A + +D R+L D++I +S DTALH + + E ++ L + G D N + FN H A +GN+ E L + ++ + +KDD F+ALHLA N H+ +LL + +ADI IRNN QT L +AVSQ + +++LV KGA+++V D +GDT LH+A+ ++ T +L + I + + ++ +A YL ++ A KN KTP+D DP ++E+L N+ K E K L+ P + VAAGG P ++S+ + + +EL+ ++Q+MED C IC DR ++ F CGH AC +CA L CH+CR+ I +I L+
Sbjct: 247 QFRVGDKVKVDLDLDILKAMQEGHGGWNPKMAEFIGKVGTVHRITERGDVRVQY---------------EGCQNRWTFHAG------ALTK--VQQFSVADIVKISENLHEVRKLQVGHGEWTDSMKAALGKIGKVLKVYADGDLRVAVNKQTWTFNPACCTVLPHGAEDLNNSMASHEREDRTTTLTALLEQLFELQLPDASGPDRLVREAAQGHIDNVKNIVTKFPDKVDQKS--AGKTALQVASHQGHIEIVKLLLSSGANLELQDEDGDTALHYASFGNQPEITEYLLSKGANINALNNGGCSTLHVAVNKQHVDCVRVL-LRHTCDVNIQDSYGDTALHDGIGKDNQEIIDLLCNYPGIDFTLRNKRGFNVLHHAALKGNKLATERILLK-ARQVADVKKDDGFSALHLAALNGHKEVAEILLTQGQADIEIRNNRKQTPLLLAVSQGHTALLELLVSKGADVNVQDEDGDTCLHLALMRQNVATELDNSPIL--AAIRSQLGMTEDDTNTGTAIACYLAQQGASLTNKNAQGKTPLDVITDPKVQEQLKQFAGVQTSTPANPAENSMPSCKVCEDDPADVKFMPCGHQISCSDCCAKMKKCLECGVPIQSKVAAGGTPIKPSSVKRQDSSNDF--SESKFQELQAKMQEMEDTVMCSICLDRKKDLVFLCGHSACHICAQPLRNCHMCRKPITKRINLF 920 HSP 2 Score: 75.485 bits (184), Expect = 5.293e-10 Identity = 42/140 (30.00%), Postives = 75/140 (53.57%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGME--WMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLT 979
KV + + +++K +Q+ HGGW M FIG+ + +I + GD+ V G + W + + K ++ DIV+I +++ ++++LQ HG W +SM+ GKI KV + GD++V V++ +NP T
Sbjct: 253 KVKVDLDLDILKAMQEGHGGWNPKMAEFIGKVGTVHRITERGDVRVQYEGCQNRWTFHAGAL-------TKVQQFSVADIVKISENLHEVRKLQVGHGEWTDSMKAALGKIGKVLKVYADGDLRVAVNKQTWTFNPACCT 385 HSP 3 Score: 62.003 bits (149), Expect = 6.509e-6 Identity = 34/125 (27.20%), Postives = 61/125 (48.80%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECL--TGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRIN 493
D++ +K Q HGGWN M E + G + G + N + ++ L + Q + V + EN E ++LQV +GEW+D M LGK ++++ + DG++++ +T+ N
Sbjct: 259 DLDILKAMQEGHGGWNPKMAEFIGKVGTVHRITERGDVRVQYEGCQNRWTFHAGALTKV---QQFSVADIVKISENLHEVRKLQVGHGEWTDSMKAALGKIGKVLKVYADGDLRVAVNKQTWTFN 380
BLAST of E3 ubiquitin-protein ligase MIB2 vs. TrEMBL
Match: A0A1S3IJ21 (E3 ubiquitin-protein ligase MIB2 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106164494 PE=4 SV=1) HSP 1 Score: 296.204 bits (757), Expect = 2.632e-82 Identity = 226/711 (31.79%), Postives = 347/711 (48.80%), Query Frame = 2
Query: 779 KLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCE------SEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNL-NEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLD-AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMD-FCDPSLKEKL-------------SVANARRMSKEIE------------------------TKILQAQGP--TTVAAGG--LMAHPGDHIDRNSAQHLKTKDE----ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
+ R GD V++ D+ +K +Q+ HGGW M GK+ V I GDV+V EG Q T G AL K V+ FS D V++ N H++++LQ GHGEW ++M+ LGK+GKV V +GDL+V + Q NP C E + + + LT ++ Q D DR I N+ N V K D V + G L +A + GHI++VKLL+ AN+ ++E+GDTALH A ++ +I + L++KGA++NA NN + + +A + +D R+L D++I +S DTALH + + E ++ L + G D N + FN H A +GN+ E L + ++ + +KDD F+ALHLA N H+ +LL + +ADI IRNN QT L +AVSQ + +++LV KGA+++V D +GDT LH+A+ ++ T +L + I + + ++ +A YL ++ A KN KTP+D DP ++E+L N+ K E K L+ P + VAAGG L A G I +S + + ++ + +EL+ ++Q+MED C IC DR ++ F CGH AC +CA L CH+CR+ I +I L+
Sbjct: 247 QFRVGDKVKVDLDLDILKAMQEGHGGWNPKMAEFIGKVGTVHRITERGDVRVQY---------------EGCQNRWTFHAG------ALTK--VQQFSVADIVKISENLHEVRKLQVGHGEWTDSMKAALGKIGKVLKVYADGDLRVAVNKQTWTFNPACCTVLPHGAEDLNNSMASHEREDRTTTLTALLEQLFELQLPDASGPDRLVREAAQGHIDNVKNIVTKFPDKVDQKS--AGKTALQVASHQGHIEIVKLLLSSGANLELQDEDGDTALHYASFGNQPEITEYLLSKGANINALNNGGCSTLHVAVNKQHVDCVRVL-LRHTCDVNIQDSYGDTALHDGIGKDNQEIIDLLCNYPGIDFTLRNKRGFNVLHHAALKGNKLATERILLK-ARQVADVKKDDGFSALHLAALNGHKEVAEILLTQGQADIEIRNNRKQTPLLLAVSQGHTALLELLVSKGADVNVQDEDGDTCLHLALMRQNVATELDNSPIL--AAIRSQLGMTEDDTNTGTAIACYLAQQGASLTNKNAQGKTPLDVITDPKVQEQLKQFAGVQTSTPANPAENSMPSCKVCEDDPADVKFMPCGHQISCSDCCAKMKKCLECGVPIQSKVAAGGQELNALSGTPIKPSSVKRQDSSNDFSESKFQELQAKMQEMEDTVMCSICLDRKKDLVFLCGHSACHICAQPLRNCHMCRKPITKRINLF 928 HSP 2 Score: 75.485 bits (184), Expect = 5.051e-10 Identity = 42/140 (30.00%), Postives = 75/140 (53.57%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGME--WMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLT 979
KV + + +++K +Q+ HGGW M FIG+ + +I + GD+ V G + W + + K ++ DIV+I +++ ++++LQ HG W +SM+ GKI KV + GD++V V++ +NP T
Sbjct: 253 KVKVDLDLDILKAMQEGHGGWNPKMAEFIGKVGTVHRITERGDVRVQYEGCQNRWTFHAGAL-------TKVQQFSVADIVKISENLHEVRKLQVGHGEWTDSMKAALGKIGKVLKVYADGDLRVAVNKQTWTFNPACCT 385 HSP 3 Score: 62.3882 bits (150), Expect = 6.312e-6 Identity = 34/125 (27.20%), Postives = 61/125 (48.80%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECL--TGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRIN 493
D++ +K Q HGGWN M E + G + G + N + ++ L + Q + V + EN E ++LQV +GEW+D M LGK ++++ + DG++++ +T+ N
Sbjct: 259 DLDILKAMQEGHGGWNPKMAEFIGKVGTVHRITERGDVRVQYEGCQNRWTFHAGALTKV---QQFSVADIVKISENLHEVRKLQVGHGEWTDSMKAALGKIGKVLKVYADGDLRVAVNKQTWTFN 380
BLAST of E3 ubiquitin-protein ligase MIB2 vs. TrEMBL
Match: A0A0S7GYI9 (MIB1 (Fragment) OS=Poeciliopsis prolifica OX=188132 GN=MIB1 PE=4 SV=1) HSP 1 Score: 290.041 bits (741), Expect = 1.533e-80 Identity = 213/735 (28.98%), Postives = 360/735 (48.98%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
I +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A ++I+N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S +++T+ + L + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 196 IWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGSSQ---------FHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKI------APSGSAVSNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKSSGQVDTR---------------------------SPSLNSNIESLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 861
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 281.952 bits (720), Expect = 3.065e-80 Identity = 214/741 (28.88%), Postives = 365/741 (49.26%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK---------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V + P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A ++I+N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ L+ + + P HI S + K + + ++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 196 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVARSGEVAAGAEGGSSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVT------KVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKDQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 905 HSP 2 Score: 170.629 bits (431), Expect = 6.195e-43 Identity = 183/736 (24.86%), Postives = 309/736 (41.98%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRNENKFK---------SEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALLDAGA---DIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + T A G+R L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S Q + +++ + + E+++ D DR G++ EV + + PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ G+ DI+ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + M LG G + +A+S +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ + L + +E LEE C++C D + FG CGH+A C +C+ + +C IC++ ++++ ++
Sbjct: 259 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGER-----LSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAAS----QNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTLIMG--------LGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSR---------------------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKDQVQSRTKI 861 HSP 3 Score: 56.6102 bits (135), Expect = 1.292e-7 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQDHK-------------IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + + +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 257 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 397 HSP 4 Score: 51.9878 bits (123), Expect = 3.346e-6 Identity = 53/241 (21.99%), Postives = 99/241 (41.08%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVM------LIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNS---MEFCSGKICKVQSILPSGDVKVN 937
G YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + M + + + +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K + Q+ ++K+ + Q++ G +N +G + KV+ IL DV VN
Sbjct: 224 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQL---LKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 459
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Cavefish
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:352910]) HSP 1 Score: 281.952 bits (720), Expect = 3.899e-80 Identity = 214/741 (28.88%), Postives = 364/741 (49.12%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETK---------ILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A ++I+N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ L+ + + P HI S + K + + ++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 193 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVARSGEVAAGAEGGSSQ---------FMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKV------APAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKDQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 902 HSP 2 Score: 170.244 bits (430), Expect = 6.664e-43 Identity = 183/736 (24.86%), Postives = 309/736 (41.98%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRNENKFK---------SEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALLDAGA---DIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + T A G+R L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S Q + +++ + + E+++ D DR G++ EV + + PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ G+ DI+ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + M LG G + +A+S +A +L A I+NK ++P+D C DPSL + L+ + + S ++ ++ + L + +E LEE C++C D + FG CGH+A C +C+ + +C IC++ ++++ ++
Sbjct: 256 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGER-----LSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAAS----QNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGSASLDIQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTLIMG--------LGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSR---------------------------SPSLNSNNETLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKDQVQSRTKI 858 HSP 3 Score: 67.0106 bits (162), Expect = 8.672e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 967 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1027 HSP 4 Score: 56.6102 bits (135), Expect = 1.267e-7 Identity = 39/148 (26.35%), Postives = 67/148 (45.27%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYDTRQDHK-------------IGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + + +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 254 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 394 HSP 5 Score: 51.9878 bits (123), Expect = 3.630e-6 Identity = 53/241 (21.99%), Postives = 99/241 (41.08%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVM------LIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNS---MEFCSGKICKVQSILPSGDVKVN 937
G YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + M + + + +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K + Q+ ++K+ + Q++ G +N +G + KV+ IL DV VN
Sbjct: 221 GTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVARSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQL---LKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 456
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 263.462 bits (672), Expect = 9.208e-74 Identity = 200/673 (29.72%), Postives = 331/673 (49.18%), Query Frame = 2
Query: 782 LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV-DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCES--------EPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAV-KNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV---RIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
++GD V+ ++ ++Q+Q+ HGGW M +I V I GDV+V + + ++P LTK V F+ G+ VR++++ +KRLQ GHGEW ++M LG+VGKV V +GDL+V GQ NP C+ + ++ T+++ L +++ Q S E D P + A G+ +VR+LV D V +G L +A + GH++VVK+L++ ++I AK+E+GD ALH ++ +I LL++KGA+VN NN+ T + +A + G D+ R+L T +AD+++ +S DT LH A+ K+F + + D NN+ FN H A +GN+ EM L R ++ + +K+D F+ALHLA NNH +L+K + D NIRNN QT L +AV+Q ++ + +LV +GA+++ D GDT +H A+ ++ +M + +R + S N + +A +L ++ A + N K+P+D S +L R S++ + LQA T ++ + H N+ +L + C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 313 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTK-------------------------VNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQ---STEQDNPG----RLVIEAAH-GSATKVRELVQKHPDKVDIKN--QGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNTELNVGAAIACFLAQEGADINYANHKGKSPLDLVTDSTTLQL----IRSFSEKHRLQQLQAITCGTSSSSSSLRRV--HTTPNTMTNLA------------MPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIR 930 HSP 2 Score: 64.3142 bits (155), Expect = 5.652e-10 Identity = 34/130 (26.15%), Postives = 68/130 (52.31%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNE--CLTGPCQLVQSLGSCAIVKGPSGNV-YRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
+++ ++ Q HGGWN M E C G + G + S N+ + + L + +T +G V ++++ E KRLQ +GEW+D M + LG+ ++++ + DG++++ + G+T+ N +
Sbjct: 324 EVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQY--SNNIRWTFHPGALTKVNT---FAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 448 HSP 3 Score: 50.447 bits (119), Expect = 9.363e-6 Identity = 46/201 (22.89%), Postives = 88/201 (43.78%), Query Frame = 2
Query: 356 FKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCI----GKRNENKFKSEKLRK--GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV 940
+++Q +G W+ M + + + + G+V+++Y LT TF GE V ++ + E +K LQ HG W + M +G+ +++K+ DGD+ V+ G W NP C+ G+ + N +E + ++ + + + Q N G V +E G KV+ ++ KV++
Sbjct: 328 LRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALT---------KVNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQSTEQDNPGRLV--IEAAHGSATKVRELVQKHPDKVDI 517
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 263.462 bits (672), Expect = 1.212e-73 Identity = 200/673 (29.72%), Postives = 331/673 (49.18%), Query Frame = 2
Query: 782 LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV-DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCES--------EPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAV-KNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV---RIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
++GD V+ ++ ++Q+Q+ HGGW M +I V I GDV+V + + ++P LTK V F+ G+ VR++++ +KRLQ GHGEW ++M LG+VGKV V +GDL+V GQ NP C+ + ++ T+++ L +++ Q S E D P + A G+ +VR+LV D V +G L +A + GH++VVK+L++ ++I AK+E+GD ALH ++ +I LL++KGA+VN NN+ T + +A + G D+ R+L T +AD+++ +S DT LH A+ K+F + + D NN+ FN H A +GN+ EM L R ++ + +K+D F+ALHLA NNH +L+K + D NIRNN QT L +AV+Q ++ + +LV +GA+++ D GDT +H A+ ++ +M + +R + S N + +A +L ++ A + N K+P+D S +L R S++ + LQA T ++ + H N+ +L + C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 319 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTK-------------------------VNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQ---STEQDNPG----RLVIEAAH-GSATKVRELVQKHPDKVDIKN--QGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNTELNVGAAIACFLAQEGADINYANHKGKSPLDLVTDSTTLQL----IRSFSEKHRLQQLQAITCGTSSSSSSLRRV--HTTPNTMTNLA------------MPSAPGPSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIR 936 HSP 2 Score: 64.3142 bits (155), Expect = 6.011e-10 Identity = 34/130 (26.15%), Postives = 68/130 (52.31%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNE--CLTGPCQLVQSLGSCAIVKGPSGNV-YRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
+++ ++ Q HGGWN M E C G + G + S N+ + + L + +T +G V ++++ E KRLQ +GEW+D M + LG+ ++++ + DG++++ + G+T+ N +
Sbjct: 330 EVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQY--SNNIRWTFHPGALTKVNT---FAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 454
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Cavefish
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:103034310]) HSP 1 Score: 261.536 bits (667), Expect = 2.640e-73 Identity = 197/676 (29.14%), Postives = 326/676 (48.22%), Query Frame = 2
Query: 782 LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV-DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCES--------EPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAV-KNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV---RIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKL--SVANARRMSKEIETKILQAQ-GPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGH-VACDVCANNLYECHICRESIENKIR 2740
++GD V+ ++ ++Q+Q+ HGGW M +I V I GDV+V + + ++P LTK V F+ G+ VR++++ +KRLQ GHGEW ++M LG+VGKV V +GDL+V GQ NP C+ + ++ T+++ L +++ Q S E D P + A G+ +VR+LV D V +G L +A + GH++VVK+L++ ++I AK+E+GD ALH ++ +I LL++KGA+VN NN+ T + +A + G D+ R+L T +AD+++ +S DT LH A+ K+F + + D NN+ FN H A +GN+ EM L R ++ + +K+D F+ALHLA NNH +L+K + D NIRNN QT L +AV+Q ++ + +LV +GA+++ D GDT +H A+ ++ +M + +R + S N + +A +L ++ A + N K+P+D S +L S + R + + + P T+ + + PG C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 258 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTK-------------------------VNTFAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSGQPGEVDANLMTAENPSESGSTVISVLEKLLSQ---STEQDNPG----RLVIEAAH-GSATKVRELVQKHPDKVDIKN--QGKTALQVAAHQGHMEVVKVLLQANSSIEAKDEDGDAALHYTAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEILTVVPNIDFTQQNNRGFNLLHHAALKGNKLATEMILSR-ARQLADVKKEDGFSALHLAALNNHRDVAEILVKEGRCDANIRNNRNQTPLQLAVTQGHVALVALLVSEGADVNAEDEEGDTAMHTALSRQQLTNMLSTSEREASALYCRLSSSGLLGNTELNVGAAIACFLAQEGADINYANHKGKSPLDLVTDSTTLQLIRSFSEKHRYVVSSSSSLRRVHTTPNTMTNLAMPSAPG------------------------------PSECLICSELALLVLFSPCQHSVACEECAHRMKKCIKCQVTITKKIR 866 HSP 2 Score: 63.929 bits (154), Expect = 7.127e-10 Identity = 34/130 (26.15%), Postives = 68/130 (52.31%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNE--CLTGPCQLVQSLGSCAIVKGPSGNV-YRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
+++ ++ Q HGGWN M E C G + G + S N+ + + L + +T +G V ++++ E KRLQ +GEW+D M + LG+ ++++ + DG++++ + G+T+ N +
Sbjct: 269 EVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQY--SNNIRWTFHPGALTKVNT---FAVGELVRVLDDIESVKRLQAGHGEWTDSMASALGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 393 HSP 3 Score: 63.1586 bits (152), Expect = 1.091e-9 Identity = 30/72 (41.67%), Postives = 42/72 (58.33%), Query Frame = 2
Query: 2531 NSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
NS QH LE+L+ R + ME+ TC IC D I + F CGH +C C+ L C ICR++I +I+L+
Sbjct: 880 NSEQH-----NLLEQLQSRYRQMEERITCPICIDNQIKLVFQCGHASCIDCSAALKTCPICRQTIRERIQLF 946
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008505.1 (pep scaffold:Pmarinus_7.0:GL477211:28706:49738:-1 gene:ENSPMAG00000007672.1 transcript:ENSPMAT00000008505.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 178.333 bits (451), Expect = 1.269e-46 Identity = 161/589 (27.33%), Postives = 273/589 (46.35%), Query Frame = 2
Query: 740 CIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDV-KVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDC-----ESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVG--TSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGA-DIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIA--------------------VRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDF-CDPSLKEKLS 2413
C+G + N+ + GD V+ D Q + LQQ+HGG+ +M + + KV ++ +GDV V + + +YNP L K + Q + Q R+S + + +FSP + LIN+ W+ ++ LG+VG+V V +GDL+V + G N NP CV D SE ++ + ++ +D + K + GN+ V+KLV + EG L +A + GHIDV + L++ A+ ++ G+TALH A ++ + LL++ A VN++N+ T + LAA G ++ R L D++ +S ++ +H A+ + E + AL D+ N F L A +G++ ++ L + K+V+ QK D FA LHLA +N + +L+K ++ +N R QTAL +A + +++ + +LV + ++DV D G+T LH+ V+IH V + +L SG+F S G E LA +L+K+ A N K P D+ P L++ L+
Sbjct: 226 CLGD-SSNRTSTMGFCLGDTVRCGVDGQTLMVLQQDHGGFSVAMALIAARPGKVAGLIDNGDVLVVYSNGGKYIYNPKCLIKVKMAQC---ISHLQLRQSFWI--VIALIFSP-SCIALINDDSI---------NWMKMVKPTLGRVGRVVKVLPSGDLRVALGGHIWNFNPACVSTADPKSLDLAETSEDSAADILKERVILVWDTDLNK----------KQLISEAAQGNVGNVQKLVQLFPGQINAQQEGRTALHVAAHQGHIDVARALLKAGASTEIGDDKGNTALHYAAHGNEAAMAGLLVSHRARVNSRNHAGRTALHLAARKGYGEVARTLC-EHACDVNTQDSTKNSPIHDAIAENYEEVIEALTSVKTLDLERQNESGFTPLQLAAFKGSKLAVQRILAKS-PKLVDEQKSDGFAPLHLAAYNGRLDVVHILIKQGRSAVNARTCGGQTALMLAAGRGHVDVVFLLVREACKLDVEDEGGNTALHLVLKEADLFSTDCHLLQDDSTIVQIHYATRVLRINRLRD-SGLF-GAGSIYAG----EALACFLVKEGANLRQTNARGKAPFDYILKPELRKTLT 780
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 122.479 bits (306), Expect = 1.501e-28 Identity = 75/260 (28.85%), Postives = 132/260 (50.77%), Query Frame = 2
Query: 1418 LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIA 2197
L + R VD + L+ + L +A + GH K+L++K AN N + NG T LHIA +K++ ++ +LL+ GA + A + +TP+ ++A G+++I +L A + N +TALH+A + E V +LL GA + A + H+ A+ G I+++ L+ ++ + + LH+A HE +LL++ A + + T LH+A + +L+++ A D NG TPLH+A
Sbjct: 58 LMQYRAPVDDI----TLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQG-GASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASP--DAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVA 310 HSP 2 Score: 114.005 bits (284), Expect = 7.329e-26 Identity = 91/309 (29.45%), Postives = 143/309 (46.28%), Query Frame = 2
Query: 1469 EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK---KRGKLLGIS---GIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPM 2377
+G+ PL A GH VV++L+E+ A I AK +NG + LH+A Q D + +L+ A V+ + +T + +AA G ++L + A+ ++ T LH+A K + LL GA I AV H+ A GN I+ + L +G N+ ALH+A +R LL+ A ++ + QT LHIA L +++L++ A D +NG TPLH+A R D + G L ++ G +S G ++A LL++ A D KN TP+
Sbjct: 5 DGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVL-LDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILL--QGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGK---MEVASLLLQRFASPDAAGKNGLTPL 307 HSP 3 Score: 106.301 bits (264), Expect = 1.602e-23 Identity = 74/270 (27.41%), Postives = 124/270 (45.93%), Query Frame = 2
Query: 1400 ALKIGNLNEVRKLVDSVGT--STLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
A ++G L V+ L+ + + + G PL +A GH DV +L+E A++ + G T LH+A + K ++ LL+ + A +A N +TP+ +A+ + + LL + A T LH+A K E LL+ GA+ A+ + HL AQ G+ + + L R VN + LHLA + +L+K+ A +N T L +A + + L++ GA+++ NG TPLH A +
Sbjct: 211 AARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVA-LLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLAR--YADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQ 477 HSP 4 Score: 104.76 bits (260), Expect = 5.379e-23 Identity = 75/256 (29.30%), Postives = 125/256 (48.83%), Query Frame = 2
Query: 1469 EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR---IHSMDTVK 2227
+G PL +A G ++V LL+++ A+ +A +NG T LH+A D K+ LL+++GA +A N TP+ +AA ++I L A+ + + LHL + HA+ + LL AD+ + HL AQ + E+ +K VN+ + L +A + + LL++ AD+N + N T LH A Q + + + +L++ GA + NG+T L IA R I +DT+K
Sbjct: 269 KGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTL-LEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGA--HVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLLKCGARPNDVTVNGNTALAIARRLGYISVVDTLK 521 HSP 5 Score: 74.7146 bits (182), Expect = 7.881e-14 Identity = 58/213 (27.23%), Postives = 109/213 (51.17%), Query Frame = 2
Query: 1871 NFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV---RIHSMDTVKKRG---KLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAA 2491
H A+ G+E+++E+ L+ +G I+ K+ + LH+A +H C++VL++Y+A ++ + TALH+A + K+L++KGA +V NG TPLHIA RI M+ + K G + + SG+ S+ G+ ++ + LL+ A + N +T + + + ++ V + R +++ K + Q P +AA
Sbjct: 6 GLTPLHCAARSGHEQVVEILLE-QGAPILAKTKNG-LSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGN---LNIVLILLQGGASPNATNVRGETALHMAARAGQTEV-VRSLLRNGAQVDAKAREEQTPLHIAA 212
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 121.324 bits (303), Expect = 2.209e-28 Identity = 87/297 (29.29%), Postives = 140/297 (47.14%), Query Frame = 2
Query: 1352 DESDEPDNDRTKTFFNALKIG---NLNEVRKLVDSVGTS---TLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTV 2224
D EP N R F L I N +V +L+ G S T G+ P+ +A GH++VV+LL++ A+ NA G+T LH+A + + ++ L+ GA V + TP+ +A+ G +I +LL R +D + + T L+ A + H + + LL+ GADI V K F H+ ++ G + + L++ + LH+A +H+ +LL A + N T LHIA + L K+L+E GAE ++ G PLH+A + D V
Sbjct: 358 DRGGEP-NARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLL-LQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMV 652 HSP 2 Score: 120.939 bits (302), Expect = 2.766e-28 Identity = 81/306 (26.47%), Postives = 143/306 (46.73%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCD 2389
G PL IA + G+++V LL+ A ++ NG T LH+A ++ + LL+++GA ++ + + +TP+ AA +G + +L R+A + + LH+A + H V LL A + V H+ A G+ R+ ++ L R G N++ + F LH+A N + +LLK+ A I+ + T +H+A +LN +++L++ GA + T+ G+TPLH+A R ++ VK LL+ AR +K + D+TP+
Sbjct: 205 GFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEML-LERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEP--NARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKT------------------------------LLQSGARVGVKAREDQTPLHIAS 477 HSP 3 Score: 114.39 bits (285), Expect = 3.134e-26 Identity = 79/251 (31.47%), Postives = 125/251 (49.80%), Query Frame = 2
Query: 1466 LEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLL---ATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEID--VTDANGDTPLHIAVR 2203
L+ + PL +A + GH V KLL+++ NA+ NG T LHIA +K++ K+ +LL+ GA ++ + +TPI +A+ G +++ +LL S NA N +T LH+A + E V LL +GA + ++ H+ ++ G I+++ L+R N+ L+ A H +LL+ ADI+ T LHIA L +L+EK A D V NG TPLH+A
Sbjct: 335 LDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNAT----NVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDP--NAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATH 579 HSP 4 Score: 111.309 bits (277), Expect = 3.423e-25 Identity = 84/284 (29.58%), Postives = 140/284 (49.30%), Query Frame = 2
Query: 1373 NDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLE-----GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+D +F A + GNL +L+D + + G+ L +A GH+DVV L+++ A I+ + G+TALHIA + I +L+ GA++NAQ+ N TP+ +AA ++I + L A+ S+ + T L +A++ H + V LL D +R A H+ A++ + + L+ E V S+ F LH+A + +LL A ++ N T LH+A + N +K+L+++GA+ID +G TPLH A R
Sbjct: 9 SDAATSFLRAARNGNL---ERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFL-LDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVR------LPALHIAARKDDTNAAALLLQHEHNADVTSKSG--FTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAAR 280 HSP 5 Score: 102.834 bits (255), Expect = 1.318e-22 Identity = 86/333 (25.83%), Postives = 156/333 (46.85%), Query Frame = 2
Query: 1409 IGNLNEVRKLVDSVGTS--TLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLD--AGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV---RIHSMDTVKKRGKLLGISGIFQST---ASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPM 2377
+G+LN V+ L+ + +S T + G PL +A G ++VVK L++ A + K T LHIA + K +I LL+ +G+D NA + TP+ A+ G D+ LL AD+S + T LH+A K E LL+ A AD + H+ ++++ + L + +S + + LH+A N +VLL+ A+ N+ LH+A + + + + +L+ + A I + G T +H+A +++ + + K + + T +S+ G+ K + +LL+ +A + K +N TP+
Sbjct: 413 MGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLL-LENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASP--HSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIK---MVNFLLRNRALVNAKTRNGYTPL 739 HSP 6 Score: 92.0485 bits (227), Expect = 3.272e-19 Identity = 90/373 (24.13%), Postives = 157/373 (42.09%), Query Frame = 2
Query: 1547 NINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGH 2665
+IN +NG ALH+A ++ + L+ + A+++ T + +AA AG DI R+L A+++ + T L++A + H E V LLD GA+ F + Q+G+E+++ + L+ + ++ + ALH+A + +LL+++ + ++ + + T LHIA +N +L+ GA +D T NG TPLH+A KRG ++ LL + A+ D K ++ TP+ S EK+ M E + IL GL S H+ T+ E L ++ + + Y P++V CGH
Sbjct: 36 DINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVL-VEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESD------TKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAA---------KRGN---------------------NNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVI-----EMLLERDAPILAK------TKNGL-----------SPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGH 349 HSP 7 Score: 75.0998 bits (183), Expect = 5.676e-14 Identity = 50/141 (35.46%), Postives = 76/141 (53.90%), Query Frame = 2
Query: 1424 EVRKLVDSVGTSTLL---EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND-TALHLAVKNFHAESVNALL 1834
EV K++ +G T + +GV PL +A GH D+V LL+ + A+I+ + G TA+H+A Q+DK + ++L ADVN TP+ +A+ G++ + L RN L + N T LH A + H VNALL
Sbjct: 617 EVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLL--RNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 755 HSP 8 Score: 53.9138 bits (128), Expect = 1.759e-7 Identity = 39/117 (33.33%), Postives = 63/117 (53.85%), Query Frame = 2
Query: 1880 AFHLVAQRGN-ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+F A+ GN ER+++ +G +N+ + ALHLA H + LLK +A+I+ TALHIA + +++LVE GA I+ NG TPL++A + + ++ VK
Sbjct: 14 SFLRAARNGNLERLMDHL---KGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVK 127
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 120.939 bits (302), Expect = 3.422e-28 Identity = 81/305 (26.56%), Postives = 143/305 (46.89%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
G PL IA + G+++V LL+ A ++ NG T LH+A ++ + LL+++GA ++ + + +TP+ AA +G + +L R+A + + LH+A + H V LL A + V H+ A G+ R+ ++ L R G N++ + F LH+A N + +LLK+ A I+ + T +H+A +LN +++L++ GA + T+ G+TPLH+A R ++ VK LL+ AR +K + D+TP+
Sbjct: 212 GFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEML-LERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEP--NARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKT------------------------------LLQSGARVGVKAREDQTPLHIA 483 HSP 2 Score: 120.553 bits (301), Expect = 4.629e-28 Identity = 87/297 (29.29%), Postives = 142/297 (47.81%), Query Frame = 2
Query: 1352 DESDEPDNDRTKTFFNALKIG---NLNEVRKLVDSVGTS---TLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTV 2224
D EP N R F L I N +V +L+ G S T G+ P+ +A GH++VV+LL++ A+ NA G+T LH+A + + ++ L+ GA V + TP+ +A+ G +I +LL R +D + + T L+ A + H + + LL+ GADI V K F H+ ++ G + + L++ + + + LH+A +H+ +LL A + N T LHIA + L K+L+E GAE ++ G PLH+A + D V
Sbjct: 365 DRGGEP-NARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLL-LQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYAS--ADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMV 657 HSP 3 Score: 116.701 bits (291), Expect = 7.253e-27 Identity = 78/249 (31.33%), Postives = 124/249 (49.80%), Query Frame = 2
Query: 1466 LEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLL---ATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
L+ + PL +A + GH V KLL+++ NA+ NG T LHIA +K++ K+ +LL+ GA ++ + +TPI +A+ G +++ +LL S NA N +T LH+A + E V LL +GA + ++ H+ ++ G I+++ L+R N+ L+ A H +LL+ ADI+ T LHIA L +L+EK A D NG TPLH+A
Sbjct: 342 LDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNAT----NVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDP--NAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATH 584 HSP 4 Score: 111.694 bits (278), Expect = 2.522e-25 Identity = 83/290 (28.62%), Postives = 141/290 (48.62%), Query Frame = 2
Query: 1373 NDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLE-----GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALL--DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKRE------GVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVR 2203
+D +F A + GNL +L+D + + G+ L +A GH+DVV L+++ A I+ + G+TALHIA + I +L+ GA++NAQ+ N TP+ +AA ++I + L A+ S+ + T L +A++ H + V LL D +R A H+ A++ + + L+ E ++N + F LH+A + +LL A ++ N T LH+A + N +K+L+++GA+ID +G TPLH A R
Sbjct: 8 SDAATSFLRAARNGNL---ERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFL-LDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLESDTKGKVR------LPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAAR 287 HSP 5 Score: 108.997 bits (271), Expect = 1.510e-24 Identity = 77/252 (30.56%), Postives = 124/252 (49.21%), Query Frame = 2
Query: 1469 EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTV 2224
+G PL IA G ++V LL+EK A+ + K +NG T LH+A D K+ LL++KGA ++ N TP+ +AA +++ ++L A+ ++ + LHLA + HA+ V LL A I A HL AQ + E+ K + VN+ + LH+A + + LL+ +A +N + N T LH A Q + + + L++ A + ANG+T L IA R+ + V
Sbjct: 541 KGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVL-LELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKA--DVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTALAIARRLGYISVV 789 HSP 6 Score: 74.7146 bits (182), Expect = 6.200e-14 Identity = 50/141 (35.46%), Postives = 76/141 (53.90%), Query Frame = 2
Query: 1424 EVRKLVDSVGTSTLL---EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND-TALHLAVKNFHAESVNALL 1834
EV K++ +G T + +GV PL +A GH D+V LL+ + A+I+ + G TA+H+A Q+DK + ++L ADVN TP+ +A+ G++ + L RN L + N T LH A + H VNALL
Sbjct: 622 EVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLL--RNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 760 HSP 7 Score: 73.1738 bits (178), Expect = 1.929e-13 Identity = 90/360 (25.00%), Postives = 148/360 (41.11%), Query Frame = 2
Query: 1640 GADVNAQNNNKITPIMLAASAGSMDI-TRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTD--------ANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTA---------SSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGH 2665
G D+N N N + + LA+ G +D+ T LL R A++ +TALH+A + V L++ GA+I A + F ++ AQ + I++ L + V ++ D F L +A+ HE + +LL+ +R ALHIA + N +L++ DVT +G TPLHIA +M+ LL + TA ++ G++ ++ LL + A+ D K ++ TP+ S EK+ M E + IL GL S H+ T+ E L ++ + + Y P++V CGH
Sbjct: 33 GMDINTCNQNGLNALHLASKEGHVDVVTELL--KREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATE--DGFTPLAVALQQGHEQVVGLLLESDTKGKVR----LPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVAT---LLLNLGAAVDFTARNGITPLHVAAKRGNN---NMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVI-----EMLLERDAPILAK------TKNGL-----------SPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGH 356 HSP 8 Score: 54.299 bits (129), Expect = 1.404e-7 Identity = 39/117 (33.33%), Postives = 63/117 (53.85%), Query Frame = 2
Query: 1880 AFHLVAQRGN-ERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+F A+ GN ER+++ +G +N+ + ALHLA H + LLK +A+I+ TALHIA + +++LVE GA I+ NG TPL++A + + ++ VK
Sbjct: 13 SFLRAARNGNLERLMDHL---KGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVK 126
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Sea Lamprey
Match: ankdd1a (ankyrin repeat and death domain containing 1A [Source:ZFIN;Acc:ZDB-GENE-070615-8]) HSP 1 Score: 115.546 bits (288), Expect = 3.163e-27 Identity = 78/250 (31.20%), Postives = 128/250 (51.20%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGAD--VNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGA--DIRAVNNKNFN---AFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAV 2200
G+ PL+IA GH++V+K L+ ++++ +NG LH A Q+ + + D + + N + TP +LAA G + + LL + A + + E +TA+HLA K+ H S+ LL+ G + VN++N N A HL A+ G+ + M L E + +N+ ++ ALH+A + HE ++L+ DIN + + LH+AV Y + LVE A I++ D T LHIA
Sbjct: 68 GMNPLLIAAWFGHLEVLKRLVNGGGRVSSQNKNGLNILHCAAQRGHVETVKYTLESLEDLGIEGKTNAEKTPYLLAAEYGQLAVLELLMDYKCAQ-DVSDGEKNTAIHLAAKSGHMLSLEHLLEKGPGRTLDFVNSQNANGNTALHLAAEGGHLECVRMLLDCEAICDINALNNESMNALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAEANINLVDCRNQTALHIAA 316 HSP 2 Score: 69.707 bits (169), Expect = 1.465e-12 Identity = 54/207 (26.09%), Postives = 100/207 (48.31%), Query Frame = 2
Query: 1343 DHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTL-------LEGVYPLMIAVNGGHIDVVKLLIEKRA--NINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLK 1936
D+ D D ++ A K G++ + L++ TL G L +A GGH++ V++L++ A +INA ALH+A + +I LLI++ D+NA + + +P+ LA I L + A++++ + N TALH+A + ES + LL AG D+ ++ + + + A+ +++M +K
Sbjct: 157 DYKCAQDVSDGEKNTAIHLAAKSGHMLSLEHLLEKGPGRTLDFVNSQNANGNTALHLAAEGGHLECVRMLLDCEAICDINALNNESMNALHMAAKHGHEQIAKLLIDETIDINAISTKQASPLHLAVQNNYPIIVTFLVEAE-ANINLVDCRNQTALHIAAETGFQESADLLLAAGTDLTLLDKQKKSCLEVAARANQVNLVDMIIK 362 HSP 3 Score: 63.5438 bits (153), Expect = 1.264e-10 Identity = 60/223 (26.91%), Postives = 101/223 (45.29%), Query Frame = 2
Query: 1637 KGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKR-EGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAE-----IDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNE 2287
K V+A+N T + AA +G D +LL R A++ G+ L +A H E + L++ G + + N N H AQRG+ ++ L+ E + I +K L LA L +L+ YK ++ + TA+H+A ++ ++ L+EKG ++ +ANG+T LH+A ++ V+ LL I A +NE
Sbjct: 24 KKVKVDAKNMRDQTALHFAAGSGQEDAVKLL-LERKANVDKGDKYGMNPLLIAAWFGHLEVLKRLVNGGGRVSSQNKNGLNILHCAAQRGHVETVKYTLESLEDLGIEGKTNAEKTPYL-LAAEYGQLAVLELLMDYKCAQDVSDGEKNTAIHLAAKSGHMLSLEHLLEKGPGRTLDFVNSQNANGNTALHLAAEGGHLECVR---MLLDCEAICDINALNNE 241
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Yeast
Match: AVO2 (Component of a complex containing the Tor2p kinase and other proteins; complex may have a role in regulation of cell growth [Source:SGD;Acc:S000004672]) HSP 1 Score: 64.6994 bits (156), Expect = 3.073e-11 Identity = 49/178 (27.53%), Postives = 82/178 (46.07%), Query Frame = 2
Query: 1988 LHLAVFNNHEGCLRVLLK-YKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYL----LKKKARTDIKNKND-----KTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAA 2491
+HLA+ HE L +LL+ + IN R N + +HIA Y C+ +L+ GA++ V D NGDTPLH+ + S+ +K LL + ++G+ KP D+A + K ++K K K P F P + NA+ ++ + +L P ++ +
Sbjct: 79 VHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMK---MLLNEGEVSLDDNVRDKGNWKPIDVAQTFEVGNIYSKVLKEVKKKGPPLGAGKKPSSFRTP-------ILNAKATFEDGPSPVLSMNSPYSLYS 246 HSP 2 Score: 60.8474 bits (146), Expect = 5.326e-10 Identity = 55/194 (28.35%), Postives = 98/194 (50.52%), Query Frame = 2
Query: 1715 ITRLLATSRNADLSIG-NSEND-TALHLAVKNFHAESVNA--LLDAGADIRAV--NNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGA---EIDVTDANGDTPLHIAV-----RIHS--MDTVKKRGKLLG 2248
+ RLL RN DL +SEN ++LH A ++H + L+ G D + K HL +G+E+ L + L+ + + +N + ++ A +H+A N++ CL +L+ AD+ + + N T LH+ + ++CMK+L+ +G + +V D P+ +A I+S + VKK+G LG
Sbjct: 21 VKRLL--RRNPDLLTNIDSENGWSSLHYA--SYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQ-QFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLLNEGEVSLDDNVRDKGNWKPIDVAQTFEVGNIYSKVLKEVKKKGPPLG 209 HSP 3 Score: 47.7506 bits (112), Expect = 5.983e-6 Identity = 26/81 (32.10%), Postives = 43/81 (53.09%), Query Frame = 2
Query: 1490 IAVNGGHIDVVKLLIEKRAN-INAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLL 1729
+A+ GH + LL+++ IN + ENG +HIA D ++ LLI GAD+ + N TP+ + GS+ ++L
Sbjct: 81 LALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKML 161
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464]) HSP 1 Score: 61.2326 bits (147), Expect = 8.319e-11 Identity = 46/157 (29.30%), Postives = 79/157 (50.32%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEK--RANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLD-AGADIRAVNNKNFNAFHLVAQRGNERILEMFL 1933
G P IA + G+++VVK L ++ + ++N G T LH+AV K F++ LI GA V ++ P+ AAS GS+ + LL + ++ + + T L A+ H ++ L++ GA+ V+NK A + NE++ + FL
Sbjct: 72 GWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAKAEDVAL---NEQVKKFFL 225 HSP 2 Score: 57.3806 bits (137), Expect = 1.557e-9 Identity = 49/163 (30.06%), Postives = 70/163 (42.94%), Query Frame = 2
Query: 1361 DEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLL----EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKG-ADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSI----GNSENDTALHLAVKNFHAESV 1822
D PD+ F A +GNL V+ L D L +GV L +AV +V + LIE A++ K++ LH A K+ +LL G + VN Q+ TP+ A + G D LL A+ + G D AL+ VK F +V
Sbjct: 66 DYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAKAEDVALNEQVKKFFLNNV 228
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374]) HSP 1 Score: 56.225 bits (134), Expect = 1.970e-8 Identity = 40/109 (36.70%), Postives = 63/109 (57.80%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRA-NINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKI--TPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALH 1789
G L IA + G DVVK +IE+ +IN ++ G+TALH A + +I +LLI GADVN ++ TP++ A++ G +D+ + L + AD +I N++ TA
Sbjct: 330 GRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLKN-GADPTIRNAKGLTAFE 437
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Yeast
Match: AKR1 (Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant [Source:SGD;Acc:S000002672]) HSP 1 Score: 53.1434 bits (126), Expect = 1.804e-7 Identity = 55/231 (23.81%), Postives = 91/231 (39.39%), Query Frame = 2
Query: 1577 TALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLK---REGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGI 2260
T LH A ++ + D L+++GADVNA+ AG++ + T LH A + + V+ LL GAD +++ FN HL N ++ L +G+ ++ + +L A + + LLK+ A I I + T LH + + +K L++ GA+ G IA M+TV + L SG
Sbjct: 111 TGLHWASINNRLSVVDFLVSQGADVNAR-------------AGAL--------------------HATPLHWAARYGYVYIVDFLLKHGADPTMTDDQGFNLLHLSVNSSNIMLVLYVLFNVVSKGLLDIDCRDPKGRTSLLWAAYQGDSLTVAELLKFGASIKIADTEGFTPLHWGTVKGQPHVLKYLIQDGADFFQKTDTGKDCFAIA---QEMNTVYSLREALTHSGF 305
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560]) HSP 1 Score: 53.1434 bits (126), Expect = 1.853e-7 Identity = 44/176 (25.00%), Postives = 79/176 (44.89%), Query Frame = 2
Query: 1472 GVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLI------NKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKF 1981
G L A G+I +V LL++ A+ K+E G +H +V + ++ N D+++++NN TP++ AA G LL + ++ ++ ALH A+ + L+ +GA+ NN+ + F L G + + E L+ G + +QKD F
Sbjct: 118 GATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLL-KFGSTVAWTDNRGFNALHCALVGGDQRVICDLILSGANFYERNNQKQDCFDLAEGMGTKSLFEQALQHHGYDRLGNQKDKLF 292 HSP 2 Score: 52.7582 bits (125), Expect = 2.584e-7 Identity = 53/207 (25.60%), Postives = 89/207 (43.00%), Query Frame = 2
Query: 1391 FFNALKIGNLNEVRKLVDSVGTST---LLEGVYPLMIAVNGGHIDVVKLLIEKRANIN-AKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSR-----NADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERIL-EMFLKREGVKIVNSQKDDKF 1981
F A K G+L V+ +V+S ++ + L A V K L+ + AN N A G TALH A + I DLL+ GAD ++ + + + + ++ + + N D+ ++ N T L A +V LL G+ + +N+ FNA H G++R++ ++ L N+QK D F
Sbjct: 54 FIEAAKDGDLKVVKDVVESGAVDINNDRIDELSGLHWACINNRFSVAKFLLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLILSGANFYERNNQKQDCF 260
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Nematostella
Match: EDO43690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXV8]) HSP 1 Score: 84.7297 bits (208), Expect = 7.632e-18 Identity = 73/267 (27.34%), Postives = 120/267 (44.94%), Query Frame = 2
Query: 1484 LMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQT-ALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK----------KRGKLLGI 2251
+ AV+ G +D VK L+ K A+IN + +G TALH A + F + +L++KGA ++ ++N T + AAS GS++I + L++ GAD+ AVNN+ L + E +L ++ +G+ + DD A + N+ + L K IN + + ALH+A S+ YL + +L++ G EI+ DA+ TPLH A D + R K++GI
Sbjct: 53 FLSAVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKY----------------------------------LIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDV-----DDARAVEEKIMLNDAQSWLN-----KNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELLSENGADFNARNKMVGI 275 HSP 2 Score: 61.6178 bits (148), Expect = 3.804e-10 Identity = 58/230 (25.22%), Postives = 107/230 (46.52%), Query Frame = 2
Query: 1388 TFFNALKIGNLNEVRKLVDSVGTSTL--LEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNS-ENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRN 2068
F +A+ G+++EV+KL+ ++G+ L A + D+VK+L++K A+++ ++ G T+LH A +I LI GADV A NN P+ LA D+ ++ D+ + E L+ A + +N + + +A H+ + +G +++ + L+ GV+I N++ D + LH A + +L + AD N RN
Sbjct: 52 VFLSAVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCIN---------ESKDKTEASALHVASSKGYLKVIGLLLQL-GVEI-NAKDADNWTPLHAAAHWGQKDACELLSENGADFNARN 270
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57]) HSP 1 Score: 87.0409 bits (214), Expect = 8.120e-18 Identity = 69/246 (28.05%), Postives = 112/246 (45.53%), Query Frame = 2
Query: 1517 VVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGN---------SENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
++ +I RA I ++++ DT L IA +K+ L+ GAD+ A++ N + AA+ G M+ + +I + N L L + S LLD GA I + N+++ H A+ G R + LKR+ I+N + LHLA + H ++ LL I+ RN L T L A ++ + IL+++ A +D TD TPLH+A R ++ VK
Sbjct: 162 IICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLEAALEHQKPVWTICDDVCLNGLVGGSNKLILVLRI------SSQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPA-IINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVK 400 HSP 2 Score: 75.0998 bits (183), Expect = 5.540e-14 Identity = 59/228 (25.88%), Postives = 106/228 (46.49%), Query Frame = 2
Query: 1577 TALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNS-----------ENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
T LH+A + L+ GAD+ + + ++TP+ A G++ + +LL + A+L G S E TALHLAV+ H + L+ GA++ AV FH E I M +K +K ++ K +K L F + + +++++A I R+ + T L IA + +++ ++ L++ GA+I D N + A M+T++
Sbjct: 6 TPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVA--AELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARECIFVM-IKVSKLKYLSFCKSEKIKREILLYFFDVVIIICYIIRFRACIECRDKDKDTPLMIAAEKNHVDTVRTLLQHGADIAAKDINDRNSVFRAAAEGCMETLE 230 HSP 3 Score: 58.9214 bits (141), Expect = 6.692e-9 Identity = 35/119 (29.41%), Postives = 67/119 (56.30%), Query Frame = 2
Query: 1481 PLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLD 1837
PL +A GH VVK L+ + I+A+ T L A + ++ ++L+++ A V+ + K TP+ LA+ G +++ +LL SR A++++ +S L +A+++ H E A++D
Sbjct: 320 PLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLL-LSRKANITLTDSAGRNCLDIAIESSHKEVAVAIID 437 HSP 4 Score: 51.2174 bits (121), Expect = 1.422e-6 Identity = 37/151 (24.50%), Postives = 71/151 (47.02%), Query Frame = 2
Query: 1469 EGVYPLMIAVNGGHIDVVKLLIEK-RANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERI 1918
E + P+ A G + V L+++ A IN ++ +T LH+A + L+ +G ++A+N TP+ AA+ G +++ +L +A + + T LHLA + H E V LL A+I ++ N + + ++ +
Sbjct: 282 EDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANIL-LDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEV 431
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Nematostella
Match: EDO39113 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB41]) HSP 1 Score: 75.8702 bits (185), Expect = 9.084e-16 Identity = 47/159 (29.56%), Postives = 79/159 (49.69%), Query Frame = 2
Query: 1469 EGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKF---KICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLK 1936
+G PL A H+D+V++L++ A+++ + + + KF + C +L+ GADVN N T + A G + LL D+++ + +N T+LH+A + LL GA+I A +N F AFHL A+ G E +L+ L+
Sbjct: 28 DGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAIESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLR 186 HSP 2 Score: 66.6254 bits (161), Expect = 1.743e-12 Identity = 55/213 (25.82%), Postives = 91/213 (42.72%), Query Frame = 2
Query: 1517 VVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNN---NKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKG 2146
V KLL N+N + +G + LH A + I +L++ GADV+ + + +I PI+ AA ++ ES LL AGAD+ N A H +RG+ +++E+ L R+G VN D +LH+A ++ LL A+I ++N+ TA H+A + ++ L+ G
Sbjct: 11 VRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDVRGSPSIQRIPPIICAAKFNAI----------------------------------ESCRVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLL-RDGQMDVNVVDRDNTTSLHVAAMIGSNPIIKTLLFCGANITAKDNDGFTAFHLAAREGEEEVLQTLLRTG 188 HSP 3 Score: 54.6842 bits (130), Expect = 1.975e-8 Identity = 53/180 (29.44%), Postives = 79/180 (43.89%), Query Frame = 2
Query: 1691 AASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKG-AEIDVTDANGDTPLHIAVRIHSMDTVK 2227
A G D R L +L+ +++ + LH A H + V LLDAGAD+ RG+ I + I+ + K FN E C RVLL+ AD+NI N QTALH A + + +++L+ G +++V D + T LH+A I S +K
Sbjct: 2 ACRDGRKDCVRKLLRGNLTNLNTLDADGFSPLHYATTTNHVDIVQMLLDAGADVDV--------------RGSPSIQRI------PPIICAAK-----------FNAIESC-RVLLRAGADVNITNCYGQTALHHATRRGHSKMVELLLRDGQMDVNVVDRDNTTSLHVAAMIGSNPIIK 149
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Nematostella
Match: EDO38725 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC11]) HSP 1 Score: 73.1738 bits (178), Expect = 1.604e-14 Identity = 68/259 (26.25%), Postives = 111/259 (42.86%), Query Frame = 2
Query: 1388 TFFNALKIGN-LNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND-TALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYL-NCMKILVEKGAEI 2155
T F+AL N V + + + ++ + PL +A G ++V +LL+EK A ++ K + G+T LH+A F L++KG ++ ++ + TP + A +AG + NA L + EN + LHLA A V AL AG + + +A H+ A G +++ LL+Y ADIN R++N T L A + CM++L + GA I
Sbjct: 7 TGFSALHHATRYNRVEAMAELIRNGSIEDLSTPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAGR---------TENAMLLLEKDENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQ-----------------------------------LLRYGADINARDSNRWTPLMCAAKTGSVKTCMELL-QSGASI 220 HSP 2 Score: 60.077 bits (144), Expect = 4.746e-10 Identity = 57/226 (25.22%), Postives = 102/226 (45.13%), Query Frame = 2
Query: 1550 INAKEENGDTALHIAVQKDKFKICDLLINKGA--DVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDT 2221
+N ++ G +ALH A + ++ + LI G+ D++ TP+ +A GSM++ RLL + A L N + +T LH+A +++ LL G I + + F G + E ++ + +++ + LHLA + L + N R+ Q+ALH+A + + L+ GA+I+ D+N TPL A + S+ T
Sbjct: 1 LNKMDDTGFSALHHATRYNRVEAMAELIRNGSIEDLS-------TPLHVACKKGSMEVGRLL-VEKGAALDKKNDKGETPLHMAACIGSFDALKFLLSKGVAIDERDFSHRTPFLSAVAAG---------RTENAMLLLEKDENQKSPLHLACAAGRALHVEALTRAGLSTNERDEEGQSALHMAALSGFRKVVTQLLRYGADINARDSNRWTPLMCAAKTGSVKT 209 HSP 3 Score: 53.1434 bits (126), Expect = 1.146e-7 Identity = 32/91 (35.16%), Postives = 48/91 (52.75%), Query Frame = 2
Query: 1955 VNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVK 2227
+N D F+ALH A N + L++ N +L T LH+A + + ++LVEKGA +D + G+TPLH+A I S D +K
Sbjct: 1 LNKMDDTGFSALHHATRYNRVEAMAELIR-----NGSIEDLSTPLHVACKKGSMEVGRLLVEKGAALDKKNDKGETPLHMAACIGSFDALK 86
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Nematostella
Match: EDO49485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG84]) HSP 1 Score: 73.559 bits (179), Expect = 1.661e-14 Identity = 60/211 (28.44%), Postives = 106/211 (50.24%), Query Frame = 2
Query: 1583 LHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRG-NERILEMFLKREGVKIVNSQKDDK--FAALHLAVFNNHEGCLRVLLKYKA-DINIRNNN--LQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIA 2197
LH+A + C L+ G DVNA++NN TP+ AA +G+++ LL S+ D + + LH A + + V +L+ AG+++ + K H A + + +E L+ G N+ K DK ++A+H A N H+ + +LL A D+ R++ T LH+A + +++L++ +++V D G TPL +A
Sbjct: 4 LHLACLGGFTECCKKLLFYGVDVNAKDNNDRTPLHCAACSGNVNCLELL-MSQAGDFNCIDKLGRNPLHYAAGHTNFRCVMSLVTAGSNVNKPDTKACTPLHYAAASDLDAKCVEHLLRNRG----NASKRDKQGYSAVHYASANGHKLAIEMLLDVSANDLLNRSSGSPCVTPLHLAAFHGHKPALEVLIKSFNDLEVRDDRGRTPLDLA 209
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Medaka
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101158637]) HSP 1 Score: 281.182 bits (718), Expect = 7.119e-80 Identity = 214/741 (28.88%), Postives = 367/741 (49.53%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKI------LQAQGPTTVAAG---GLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A ++I+N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DP+L + L+ + + S ++ T+ +++ V + + P HI S + K + ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 197 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGSSQ---------FHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKI------APSGSAVSNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNLCKALAKCHKEKSSGQVGTRSPSLNSNMESLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 906 HSP 2 Score: 169.474 bits (428), Expect = 1.461e-42 Identity = 183/736 (24.86%), Postives = 310/736 (42.12%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALL---DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + + + A G+R L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S Q + +++ + + E+++ D DR G++ EV + + + PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ +A DI+ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + M LG G + +A+S +A +L A I+NK ++P+D C DP+L + L+ + + S ++ T+ + L + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 260 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSNASGER-----LSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAAS----QNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTLIMG--------LGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPNLCKALAKCHKEKSSGQVGTR---------------------------SPSLNSNMESLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 862 HSP 3 Score: 67.0106 bits (162), Expect = 8.169e-11 Identity = 25/61 (40.98%), Postives = 44/61 (72.13%), Query Frame = 2
Query: 2564 ELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
++++L+Q++QD++++ C +C DR N+ F CGH C +C + + EC ICR++IE +I LY
Sbjct: 960 DVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1020 HSP 4 Score: 56.6102 bits (135), Expect = 1.256e-7 Identity = 41/149 (27.52%), Postives = 68/149 (45.64%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYD--------------TRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + Q H +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 258 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFH-VGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 398 HSP 5 Score: 51.2174 bits (121), Expect = 5.324e-6 Identity = 53/241 (21.99%), Postives = 101/241 (41.91%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXK------VMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNS---MEFCSGKICKVQSILPSGDVKVN 937
G+ YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + V + + + +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K + + Q+ ++K+ + Q++ G +N +G + KV+ IL DV VN
Sbjct: 225 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSNASGERLSQL---LKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 460
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Medaka
Match: mib1 (mindbomb E3 ubiquitin protein ligase 1 [Source:NCBI gene;Acc:101158637]) HSP 1 Score: 280.026 bits (715), Expect = 3.609e-79 Identity = 214/741 (28.88%), Postives = 367/741 (49.53%), Query Frame = 2
Query: 677 ILKDGDMYVSRLGMEWMINPKCI----------------GKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSME---FCSGKICKVQS------ILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVN----QLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV--DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFL-KREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLL-KYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSN--------EGSDK--PEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKI------LQAQGPTTVAAG---GLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGHV-ACDVCANNLYECHICRESIENK 2734
+ +G + R+G E M + KC+ G++N N+ L+ GD+V I D++ ++ LQ HGGW + M +G +C + PSG+ +NP +LTK ++ AA + S F GD V++ + +IK LQ+GHGEW AM LGKVG+V+ + + DLKV++ G NP V P + N +L+Q++ + + ES + + + K N G+L +V ++ V + G + A GH+DV+KLL++ ++ A++++GD A+H A D+ + ++L GAD+NA+N + TP+ +A + G + + + L S+ +SE DT LH A+ + ++ LL+AGAD+ NN FNA H A RGN + + L K IV+ +KDD + ALHLA NNH +L+ + A ++I+N N QTALH+AV +++ +++LV A++DV D +GDTPLH A+R H++ +++ + +S + S N +G++K +A +L A I+NK ++P+D C DP+L + L+ + + S ++ T+ +++ V + + P HI S + K + ++++Q + C++C D+ V F CGH+ AC+ CA+ + +C CR +E +
Sbjct: 197 LWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNR-NPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGN--------RWTFNPAVLTKANVVRSGEVAAGAEGGSSQ---------FHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKI------APSGSAVSNASGERLSQLLKKLFETQESGDINEELVKAAAN----GDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKTL-LDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPNLCKALAKCHKEKSSGQVGTRSPSLNSNMESLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLI--CKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERR 906 HSP 2 Score: 169.474 bits (428), Expect = 1.464e-42 Identity = 183/736 (24.86%), Postives = 310/736 (42.12%), Query Frame = 2
Query: 590 EVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRL-GMEWMINPKCIGKRN---------ENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSEND--TALHLAVKNFHAESVNALL---DAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFG-CGHVA-CDVCANNLYECHICRESIENKIRL 2743
E++++LQ HGGW + M + + I +D D+ V G W NP + K N + S + GD+VQIC DI +IK LQ+ HG W +M GK+ +VQ I D+KV V YNP +TK + + + A G+R L +L K+F + + N+ +K G + ++ +L K VDVNG +C T S Q + +++ + + E+++ D DR G++ EV + + + PL IAVN GH+ VVK L++ + + ++ GDT LH A+ K + + +L+ GADV NNN + AA G+ R+L + + ++D TALHLA N H E L+ +A DI+ VN + A HL +R + +I +R+L++ +A +++++ + T LH A+ L+ ++ L D+ D + P + M LG G + +A+S +A +L A I+NK ++P+D C DP+L + L+ + + S ++ T+ + L + E LEE C++C D + FG CGH+A C +C+ + +C IC+E ++++ ++
Sbjct: 260 EIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSNASGER-----LSQLLKKLFETQESGDI--NEELVKAAANGD---LAKVEDIL----KRPDVDVNG---------------QCAGHTAMQAAS----QNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLQRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQ--TALHLAVERQHTQI-----------------------------------VRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQ------DMQDVSKVEPWEPSKNTLIMG--------LGTQGAEKKSAAS---------IACFLAANGADLTIRNKKGQSPLDLCPDPNLCKALAKCHKEKSSGQVGTR---------------------------SPSLNSNMESLEE-------------CMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKI 862 HSP 3 Score: 56.9954 bits (136), Expect = 9.321e-8 Identity = 41/149 (27.52%), Postives = 68/149 (45.64%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNECLTGPCQLVQSLGS-CAI-------VKGPSGNVYRLNSELLIQYD--------------TRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
D+E ++ Q HGGW M E LT + G+ C I V+ PSGN + N +L + + + Q H +G V + + + K LQ +GEW++ M LGK + Q + D ++K++ G ++ N A+
Sbjct: 258 DLEIVQSLQHGHGGWTDGMFETLT-------TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFH-VGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAV 398 HSP 4 Score: 52.373 bits (124), Expect = 2.622e-6 Identity = 53/241 (21.99%), Postives = 101/241 (41.91%), Query Frame = 2
Query: 254 GNVYRLNSELLIQYDTRQDH---KIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKY-RGRTFRINSKAILTDNCKKTSMXXXXXXXXXXK------VMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNS---MEFCSGKICKVQSILPSGDVKVN 937
G+ YR + +L + + ++ +IG V + + E + LQ +G W+D M L + +D ++ ++Y G + N + N ++ + V + + + +K LQ+ HG W E M +G+ + +I D D+ V G W NP + K + + Q+ ++K+ + Q++ G +N +G + KV+ IL DV VN
Sbjct: 225 GSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSNASGERLSQL---LKKLFETQES--GDINEELVKAAANGDLAKVEDILKRPDVDVN 460
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 263.077 bits (671), Expect = 5.647e-74 Identity = 209/691 (30.25%), Postives = 341/691 (49.35%), Query Frame = 2
Query: 782 LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV-DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESE------PDIQ--TMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAV-KNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDL---------------------AIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGH-VACDVCANNLYECHICRESIENKIR 2740
++GD V+ ++ ++Q+Q+ HGGW M +I V I GDV+V + + ++P LTK V F G+ VR+++ +KRLQ GHGEW ++M VLG+VGKV V +GDL+V GQ NP C+ A D ++ P+ T+++ L +++ QS E D P + A G+ +VR+LV D V +G L +A + GH++VVK L++ ++ K+E+GDTALH A ++ +I LL++KGA+VN NN+ T + +A + G D+ R+L T +AD+++ +S DT LH A+ K+F + ++ D N++ FN H A +GN+ E L R ++V+ +KDD F+ALHLA NNH VL+K + DIN+RNN QTAL +AV+Q + +++LV++GA++++ D +GDT +H+A+ + V +L + +T ++EG P L A +L ++ A + N K+P+D L + ++ + S++ + LQA T G + S + + T + L E C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 249 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTK-------------------------VNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKLLSQST---EQDNP----CRLVIEAAH-GSAVKVRELVQKHPDKVDIKN--QGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQLANV-----MLSPAVGTGATEDNSEGC-SPTTLHGRLSSSGLLGNGELSVGTAIACFLAQEGADINYANHKGKSPLDL----LADSGALQLIKSFSEKHRLQRLQA---ITCGQG---------LSSASLRRVHTTPNTMTNLVLPTPPGPSE--CLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 878 HSP 2 Score: 63.5438 bits (153), Expect = 9.674e-10 Identity = 34/130 (26.15%), Postives = 67/130 (51.54%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNE--CLTGPCQLVQSLGSCAIVKGPSGNV-YRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
+++ ++ Q HGGWN M E C G + G + S N+ + + L + +T +G V +++ E KRLQ +GEW+D M +LG+ ++++ + DG++++ + G+T+ N +
Sbjct: 260 EVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQY--SNNIRWTFHPGALTKVNT---FGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 384
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 263.462 bits (672), Expect = 1.168e-73 Identity = 209/691 (30.25%), Postives = 341/691 (49.35%), Query Frame = 2
Query: 782 LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV-DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESE------PDIQ--TMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAV-KNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDL---------------------AIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGH-VACDVCANNLYECHICRESIENKIR 2740
++GD V+ ++ ++Q+Q+ HGGW M +I V I GDV+V + + ++P LTK V F G+ VR+++ +KRLQ GHGEW ++M VLG+VGKV V +GDL+V GQ NP C+ A D ++ P+ T+++ L +++ QS E D P + A G+ +VR+LV D V +G L +A + GH++VVK L++ ++ K+E+GDTALH A ++ +I LL++KGA+VN NN+ T + +A + G D+ R+L T +AD+++ +S DT LH A+ K+F + ++ D N++ FN H A +GN+ E L R ++V+ +KDD F+ALHLA NNH VL+K + DIN+RNN QTAL +AV+Q + +++LV++GA++++ D +GDT +H+A+ + V +L + +T ++EG P L A +L ++ A + N K+P+D L + ++ + S++ + LQA T G + S + + T + L E C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 318 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTK-------------------------VNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKLLSQST---EQDNP----CRLVIEAAH-GSAVKVRELVQKHPDKVDIKN--QGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQLANV-----MLSPAVGTGATEDNSEGC-SPTTLHGRLSSSGLLGNGELSVGTAIACFLAQEGADINYANHKGKSPLDL----LADSGALQLIKSFSEKHRLQRLQA---ITCGQG---------LSSASLRRVHTTPNTMTNLVLPTPPGPSE--CLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 947 HSP 2 Score: 63.5438 bits (153), Expect = 9.491e-10 Identity = 34/130 (26.15%), Postives = 67/130 (51.54%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNE--CLTGPCQLVQSLGSCAIVKGPSGNV-YRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
+++ ++ Q HGGWN M E C G + G + S N+ + + L + +T +G V +++ E KRLQ +GEW+D M +LG+ ++++ + DG++++ + G+T+ N +
Sbjct: 329 EVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQY--SNNIRWTFHPGALTKVNT---FGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 453
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Medaka
Match: mib2 (mindbomb E3 ubiquitin protein ligase 2 [Source:NCBI gene;Acc:101175479]) HSP 1 Score: 263.462 bits (672), Expect = 2.033e-73 Identity = 209/691 (30.25%), Postives = 341/691 (49.35%), Query Frame = 2
Query: 782 LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNV-DRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESE------PDIQ--TMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLV----DSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAV-KNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDL---------------------AIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTF-GCGH-VACDVCANNLYECHICRESIENKIR 2740
++GD V+ ++ ++Q+Q+ HGGW M +I V I GDV+V + + ++P LTK V F G+ VR+++ +KRLQ GHGEW ++M VLG+VGKV V +GDL+V GQ NP C+ A D ++ P+ T+++ L +++ QS E D P + A G+ +VR+LV D V +G L +A + GH++VVK L++ ++ K+E+GDTALH A ++ +I LL++KGA+VN NN+ T + +A + G D+ R+L T +AD+++ +S DT LH A+ K+F + ++ D N++ FN H A +GN+ E L R ++V+ +KDD F+ALHLA NNH VL+K + DIN+RNN QTAL +AV+Q + +++LV++GA++++ D +GDT +H+A+ + V +L + +T ++EG P L A +L ++ A + N K+P+D L + ++ + S++ + LQA T G + S + + T + L E C+IC + + V F C H VAC+ CA+ + +C C+ +I KIR
Sbjct: 374 FQQGDKVKCLLEVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQYSNNIRWTFHPGALTK-------------------------VNTFGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACLSAQPVDVDANLMTTENPNESGSTVISVLEKLLSQST---EQDNP----CRLVIEAAH-GSAVKVRELVQKHPDKVDIKN--QGKTALQVAAHQGHMEVVKALLQANCSVEVKDEDGDTALHYAAFGNQAEIARLLLSKGANVNLLNNSMCTALHIAVNKGFTDVVRVL-TEHSADVNLQDSYGDTPLHDAIAKDFRSIIEMLVVVPSIDFTQQNHRGFNLLHHAALKGNKLATEKILAR-ARQLVDVKKDDGFSALHLAALNNHRDVAEVLVKEGRCDINVRNNRNQTALQLAVTQGHTELVQLLVDEGADVNMEDEDGDTAMHVALLRPQLANV-----MLSPAVGTGATEDNSEGC-SPTTLHGRLSSSGLLGNGELSVGTAIACFLAQEGADINYANHKGKSPLDL----LADSGALQLIKSFSEKHRLQRLQA---ITCGQG---------LSSASLRRVHTTPNTMTNLVLPTPPGPSE--CLICSELALLVLFCPCQHSVACEECAHRMKKCIKCQVTITKKIR 1003 HSP 2 Score: 63.5438 bits (153), Expect = 9.410e-10 Identity = 34/130 (26.15%), Postives = 67/130 (51.54%), Query Frame = 2
Query: 125 DMETIKIKQREHGGWNANMNE--CLTGPCQLVQSLGSCAIVKGPSGNV-YRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAI 505
+++ ++ Q HGGWN M E C G + G + S N+ + + L + +T +G V +++ E KRLQ +GEW+D M +LG+ ++++ + DG++++ + G+T+ N +
Sbjct: 385 EVDILRQMQEGHGGWNPKMAEYICRIGTVHRITDRGDVRVQY--SNNIRWTFHPGALTKVNT---FGVGELVRVLDEVETVKRLQAGHGEWTDSMTPVLGQVGKVLKVYADGDLRVAFGGQTWTFNPACL 509
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Planmine SMEST
Match: SMESG000017279.1 (SMESG000017279.1) HSP 1 Score: 1786.54 bits (4626), Expect = 0.000e+0 Identity = 879/894 (98.32%), Postives = 885/894 (98.99%), Query Frame = 2
Query: 68 MGGAESVENSNIQVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK 2749
MGG ESVENSNIQVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSMEMEETFEEGEKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAAR QRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVN GHIDV+KLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVN LLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDT LHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKND+TPMDFCDPSLKEKLSVANARRMSKEIETKILQAQG TTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDE+TCIIC+DR NVTFGCGHVACDVCANNLYECHICRE+I NKIRLY+
Sbjct: 1 MGGVESVENSNIQVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSMEMEETFEEGEKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARAQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNEGHIDVIKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNVLLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTALHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDRTPMDFCDPSLKEKLSVANARRMSKEIETKILQAQGSTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDERTCIICFDRQNNVTFGCGHVACDVCANNLYECHICRENIGNKIRLYR 894
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Planmine SMEST
Match: SMESG000017281.1 (SMESG000017281.1) HSP 1 Score: 1026.54 bits (2653), Expect = 0.000e+0 Identity = 545/908 (60.02%), Postives = 675/908 (74.34%), Query Frame = 2
Query: 68 MGGAESVENSNIQVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQY--DTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMG-QSDHSDESDE-PDNDRTKT---------FFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK 2749
MG + S + VVI KD+ETI Q HGGWN +MNECL GPC+LVQ +GS AIV+GPSGNVYRLNSELL +Y DT Q+ G V LI++ ++F++LQV +GEW+++M +I+GKKV++I +F DG+VKIKY G TF IN KAI + N K E+ F+ GEKV+LI N + MK LQ++HGGW +L+ L+ G+EVEIV+I +DGDMYVS+ GMEWMINP+CI K KLRKGD V + DDIQK KQLQQNHGGWV+ M +GK K+++ILP+GDVKVNV+ L LVYNP+LLTK + K ++K+F+PGDKVRLIN+K K+K LQK HGEWV++M+F LGK GKVKS+ +GDL+VKIM Q L LNPECVEATDSD +S+PDI +M+ L+++M SD SD+ D+ D+D KT F A+K G LN VR L +SVG + L+G+YPLMIAVN GHIDVVKLLIEKRAN+NAK+++GDTALH+AV K++ KICDLLINKGADVNAQNNNKITPIMLAAS+GS++ITRLLATSRNADLSIG+S+NDTALHLAV+ SV LLDAGADI A+N K FN FHL A +G+ +ILE+FLKREGVKIV+SQK+DKFAA+HL NNHE CLRVLL YKADINIRNN LQTALHIAV QK+LNC+KILVE AEI++ D++GDTPLHIA+++ ++ KK G++ G+ + EGSD P+D+AIYLLK A D+KNK TPM+ C D LK ++ N +K+ +++ + A A + ++K++DEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK
Sbjct: 1 MGASLSGLKAGDDVVIAIKDLETIIQMQMAHGGWNDSMNECLIGPCKLVQGIGSDAIVRGPSGNVYRLNSELLSKYGSDTSQESISGYVVELIQDEKQFRQLQVGHGEWNNNMKDIIGKKVQVIHKFLDGDVKIKYNGSTFVINPKAIASKNEKPPVSEVCHKFKIGEKVVLIGNVDTMKKLQENHGGWSDLLMLYAGKEVEIVEIFEDGDMYVSKFGMEWMINPECI------DVKDNKLRKGDTVILSDDIQKTKQLQQNHGGWVSRMSVFAGKCGKIEAILPTGDVKVNVNGLGLVYNPLLLTKSD--------------------KSDLKMFAPGDKVRLINDKSKVKLLQKNHGEWVSSMEFFLGKKGKVKSLFADGDLRVKIMNQNLTLNPECVEATDSDSDSDPDIFSMMEHLSKLMNLDSDQSDQEDKGKDSDDGKTDGNQEEVMKLFFAVKNGELNNVRNLCESVGVNARLKGIYPLMIAVNEGHIDVVKLLIEKRANLNAKDKDGDTALHLAVPKNQIKICDLLINKGADVNAQNNNKITPIMLAASSGSVEITRLLATSRNADLSIGDSDNDTALHLAVRKSRVNSVEILLDAGADINALNKKLFNGFHLAALQGHAKILELFLKREGVKIVDSQKEDKFAAIHLVALNNHEDCLRVLLAYKADINIRNNILQTALHIAVLQKHLNCVKILVENYAEINLRDSDGDTPLHIAMKVKQIEAFKKMGRMFGMGSLMGILGDMTEGSDTPDDIAIYLLKHGAGLDMKNKKGLTPMEMCNDDLLKARILSLNIPNSTKQ---DLIEKEPNPGTKAERFEAPIAPIRQLSPPPNIKSRDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLYK 879
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Planmine SMEST
Match: SMESG000031930.1 (SMESG000031930.1) HSP 1 Score: 484.182 bits (1245), Expect = 3.399e-160 Identity = 288/647 (44.51%), Postives = 397/647 (61.36%), Query Frame = 2
Query: 815 DIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQ--RRRSSALEKLE-VKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQKLNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLEGVYPLMIAVNGGHIDVVKLLIEKRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNERILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKYKADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC-DPSLKEKLSVANARRMSKEIETKILQAQGPTTVAAGGLMAHPGDHIDRNSAQHLKTKDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRL 2743
D+ I + Q+NHGGW + M+ C CK+ +N D G+ A G R S L + VK G V+LI ++ + K LQ HGEW M+ +LGK +V + +GD K+ + ++P+ +++ E + + + +D S +DE RT F ALK GNL+EV +LVDSVG +T L+G+YPL+IA N GH D+V LL+EK+ANIN +++GDTALH+AV +++FK+CDLL+ KGADVNAQNN KITP M+A GS DI LL TS NADLSIG+S+ DTALHLAV + ESV ALL+AGADI +N K FNA+HL A G+E+ILE+FLKR+G K+VNSQK+DKFAA+H+A NNHE CL VL+KYKAD+N++N L+TALHIAV +KYL+ +K LVE AE+D+ D NG+T LH+A++++ ++ G L ++ + EG DK ED+AIYL++ A DIKN KTP+D C D SLK ++ R K TK L+ HP D ++ + EL ELRQ++++MEDEK C IC+DR + V F CGH+ CD C++N+ ECH+C E I +KI++
Sbjct: 20 DLSTIIKKQKNHGGWNDLMKECLTGPCKL----------INDD---------------GSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKS-----EIVENTELKSDDSDDSDDSNDRSLITDE----RTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVNLLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSIGDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHLAAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENKAELDLKDKNGNTALHVAMKVNQTES--GFGNFLKMTSALGVLGNIGEGLDKLEDIAIYLIENGASIDIKNNKGKTPLDICNDNSLKTRILSLVERDSQKHHITK------------KELLEHPVQPSDA------RSNEAELMELRQKLKEMEDEKICKICHDRRLKVIFECGHMCCDDCSDNISECHLCSEKILHKIKI 612 HSP 2 Score: 162.155 bits (409), Expect = 1.702e-41 Identity = 79/156 (50.64%), Postives = 106/156 (67.95%), Query Frame = 2
Query: 68 MGGAESVENSNIQVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQSLGSCAIVKGPSGNVYRLNSELLIQYDTRQDHKIGSFVTLIENAEEFKRLQVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILTDNCKKTSM 535
MG S + +VVI +D+ TI KQ+ HGGWN M ECLTGPC+L+ GS A V GPSG +YRLNSELL+ + + + HKIGSFV LI++ EEFK LQ +GEW +DM NILGK+VE+IQ F+DG+ KI + F ++ K I ++ + T +
Sbjct: 1 MGANISSFDIRKKVVIANQDLSTIIKKQKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTEL 156 HSP 3 Score: 61.2326 bits (147), Expect = 3.108e-9 Identity = 100/442 (22.62%), Postives = 183/442 (41.40%), Query Frame = 2
Query: 608 QKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCIGKRNENKFKSEKLRK-GDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTK--CEGNQANP---------------TAARGQR----RRSSALEKLEVKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGD--LKVKIMGQKLNLNPECV-EATDSDCESEPDIQ-TMV------NQLTQIMGQSDHSDESDEPDNDRTKTFFNALKIGNLNEVRKLVDSVGTSTLLE----GVYPLMIAVNGGHIDVVKLLIEKRAN--INAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAES 1819
QK+HGGW +LM + +++ + GM + +N + + FKS K K G VQ+ D ++ K LQ HG W M GK +V I GD K++ + + +P + E + T R R ++ L ++ V S G L + +GH + VN +L K + D +GD L + + + L + + D + ++ I TM+ + +++ S+++D S D+DR A+ L V L+++ LL Y L A GH ++++ +++ +N+++E+ A+H+A + +L+ AD+N QN T + +A + + L ++ A+L + + +TALH+A+K ES
Sbjct: 28 QKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVP-----FKSVKKHKIGSFVQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTIKSEIVENTELKSDDSDDSDDSNDRSLITDERTMRFLVALKTGNLSEVLRLVDSVGVNTNLKGMYPLLIAANEGHEDLVN---LLLEKKANINIADKDGDTALHLAVHENRFKLCDLLLKKGADVNAQNNKKITPTMIAVLKGSQDILELLITSNNADLSI-GDSDRDTALHLAVNSSRLESVEALLNAGADIYLLNKKLFNAYHL--AAISGHEKILEIFLKRDGEKLVNSQKEDKFAAIHMAAMNNHEHCLPVLMKYKADLNLQNIELRTALHIAVKRKYLSFVKHLVENK-AELDLKDKNGNTALHVAMKVNQTES 457 HSP 4 Score: 55.0694 bits (131), Expect = 2.383e-7 Identity = 38/127 (29.92%), Postives = 62/127 (48.82%), Query Frame = 2
Query: 368 QVQNGEWSDDMGNILGKKVEIIQQFQDGNVKIKYRGRTFRINSKAILT-DNCKKTSMXXXXXXXXXXKVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYVSRLGMEWMINPKCI 745
Q +G W+D M L ++I + G +R+NS+ ++ + KK + V LI++ E K LQ HG W E M +G+EVE+++I KDGD +S ++++PK I
Sbjct: 28 QKNHGGWNDLMKECLTGPCKLINDDGSDATVMGPSGMIYRLNSELLVPFKSVKKHKIGSF--------VQLIQDEEEFKLLQNTHGEWKEDMRNILGKEVEVIQIFKDGDYKISHNNEIFIVHPKTI 146
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Planmine SMEST
Match: SMESG000030607.1 (SMESG000030607.1) HSP 1 Score: 224.172 bits (570), Expect = 4.509e-60 Identity = 183/663 (27.60%), Postives = 306/663 (46.15%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYV-SRLGMEWMINPKCIGK-----RNENKFKSEK--------LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLE--VKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQK-------LNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEP------------------------DNDRTKTFFNALKIGNLNEVRKLVD--SVGTSTLLEGVYPLMIAVNGGHIDVVKLLIE---KRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNE---RILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
+V++ + ++++NLQ HGGW + M + E + +I +DGD+ V G ++ NP C+ K EN S++ LR G +I + + + + S +P D YNP + PT+ Q R SS L + SPG V++ ++ IK +Q G G W +M +G VGK+ + DL ++ + LN + S S P++ Q ++ + + +P D + F A G+L++V +L+ SV +++ L A GH+++V+ LI+ + +IN K+ +GD A+H A D I +L N GAD+N++N TP+ +A + G +++ + L ++A + + + DT LH ++ H + AL+ A +DI NN FN+ H A RGN RI+ L R+ IV+ +KDD + ALHLA NNH +L+K AD+N++N NLQT LH+AV +++ N +K+LV+ GA+I + D +GDTPLH A+R H++ +++ L + + + E +A YL+ + IKNK +TP+D C
Sbjct: 250 QVVIDLDFDIVRNLQVGHGGWADCMMECLSMEGTVTQIDEDGDVQVIFSSGAQYTFNPVCLTKTTRRDHGENSLSSQRSTITVPITLRPGAT-----NISRTRSTESDD--------------VPTSSTVPDSDTTR--------YNPHNPRR----STLPTSVYAQHRDSSQSRPLSHAPESLSPGCWVKISSDLELIKTVQCGLGMWNESMAVCVGTVGKIVQ-SLGHDLWRVVLSRTSGSSESTFVLNSALLARIRSP-MSSPEVSVSATQPRTMLLSQRSTGSTSQPIEAVEAASTGTLSHVLRSIFEAHTTGDAKELFVKAAANGDLDKVLELLKKRSVDVNSMFARHSALHAASQNGHVEIVQALIKYGGDQLDINLKDPDGDAAVHHAALGDHKIIIQILKNAGADINSRNRRNQTPLHIAVNKGHLEVLKALLELKSALQTNQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQW--IVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVDSGADISIPDKDGDTPLHEALRHHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSAAH-IACYLVSNGSDIHIKNKKGQTPLDLC 876 HSP 2 Score: 68.1662 bits (165), Expect = 2.757e-11 Identity = 28/64 (43.75%), Postives = 42/64 (65.62%), Query Frame = 2
Query: 2555 KDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
KD++ ++L Q+ QD+++ C +C DR + F CGH +C +C + L C ICRE+I NKI LY
Sbjct: 1230 KDKDYKKLEQQYQDIKEMTMCPVCMDRVRKMVFMCGHCSCQLCGDKLETCPICRETITNKILLY 1293
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Planmine SMEST
Match: SMESG000030607.1 (SMESG000030607.1) HSP 1 Score: 221.09 bits (562), Expect = 4.654e-59 Identity = 183/663 (27.60%), Postives = 305/663 (46.00%), Query Frame = 2
Query: 566 KVMLIRNSEVMKNLQKDHGGWCELMNLFIGEEVEIVKILKDGDMYV-SRLGMEWMINPKCIGK-----RNENKFKSEK--------LRKGDIVQICDDIQKIKQLQQNHGGWVNSMEFCSGKICKVQSILPSGDVKVNVDRLELVYNPMLLTKCEGNQANPTAARGQRRRSSALEKLE--VKVFSPGDKVRLINNKHKIKRLQKGHGEWVNAMQFVLGKVGKVKSVDVNGDLKVKIMGQK-------LNLNPECVEATDSDCESEPDIQTMVNQLTQIMGQSDHSDESDEP------------------------DNDRTKTFFNALKIGNLNEVRKLVD--SVGTSTLLEGVYPLMIAVNGGHIDVVKLLIE---KRANINAKEENGDTALHIAVQKDKFKICDLLINKGADVNAQNNNKITPIMLAASAGSMDITRLLATSRNADLSIGNSENDTALHLAVKNFHAESVNALLDAGADIRAVNNKNFNAFHLVAQRGNE---RILEMFLKREGVKIVNSQKDDKFAALHLAVFNNHEGCLRVLLKY-KADINIRNNNLQTALHIAVSQKYLNCMKILVEKGAEIDVTDANGDTPLHIAVRIHSMDTVKKRGKLLGISGIFQSTASSNEGSDKPEDLAIYLLKKKARTDIKNKNDKTPMDFC 2386
+V++ + ++++NLQ HGGW + M + E + +I +DGD+ V G ++ NP C+ K EN S++ LR G +I + + + + S +P D YNP + PT+ Q R SS L + SPG V++ ++ IK +Q G G W +M +G VGK+ + DL ++ + LN + S S P++ Q ++ + + +P D + F A G+L++V +L+ SV +++ L A GH+++V+ LI+ + +IN K+ +GD A+H A D I +L N GAD+N++N TP+ +A + G +++ + L + S+ + + DT LH ++ H + AL+ A +DI NN FN+ H A RGN RI+ L R+ IV+ +KDD + ALHLA NNH +L+K AD+N++N NLQT LH+AV +++ N +K+LV+ GA+I + D +GDTPLH A+R H++ +++ L + + + E +A YL+ + IKNK +TP+D C
Sbjct: 250 QVVIDLDFDIVRNLQVGHGGWADCMMECLSMEGTVTQIDEDGDVQVIFSSGAQYTFNPVCLTKTTRRDHGENSLSSQRSTITVPITLRPGAT-----NISRTRSTESDD--------------VPTSSTVPDSDTTR--------YNPHNPRR----STLPTSVYAQHRDSSQSRPLSHAPESLSPGCWVKISSDLELIKTVQCGLGMWNESMAVCVGTVGKIVQ-SLGHDLWRVVLSRTSGSSESTFVLNSALLARIRSP-MSSPEVSVSATQPRTMLLSQRSTGSTSQPIEAVEAASTGTLSHVLRSIFEAHTTGDAKELFVKAAANGDLDKVLELLKKRSVDVNSMFARHSALHAASQNGHVEIVQALIKYGGDQLDINLKDPDGDAAVHHAALGDHKIIIQILKNAGADINSRNRRNQTPLHIAVNKGHLEVLKALLELK-CLASLQDYDGDTPLHDSITRRHDKLTLALVQANSDITVCNNAGFNSLHHAALRGNPNATRIILENLPRQW--IVDEKKDDGYTALHLAALNNHADVAELLIKVGSADLNVQNINLQTPLHLAVERQHSNMVKLLVDSGADISIPDKDGDTPLHEALRHHTLSQLRQMQDLQDMGKLLMGLSLGGEKRSAAH-IACYLVSNGSDIHIKNKKGQTPLDLC 875 HSP 2 Score: 68.1662 bits (165), Expect = 2.925e-11 Identity = 28/64 (43.75%), Postives = 42/64 (65.62%), Query Frame = 2
Query: 2555 KDEELEELRQRIQDMEDEKTCIICYDRPINVTFGCGHVACDVCANNLYECHICRESIENKIRLY 2746
KD++ ++L Q+ QD+++ C +C DR + F CGH +C +C + L C ICRE+I NKI LY
Sbjct: 1229 KDKDYKKLEQQYQDIKEMTMCPVCMDRVRKMVFMCGHCSCQLCGDKLETCPICRETITNKILLY 1292 The following BLAST results are available for this feature:
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase MIB2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004508 ID=SMED30004508|Name=E3 ubiquitin-protein ligase MIB2|organism=Schmidtea mediterranea sexual|type=transcript|length=2842bpback to top protein sequence of SMED30004508-orf-1 >SMED30004508-orf-1 ID=SMED30004508-orf-1|Name=SMED30004508-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=895bp MGGAESVENSNIQVVIGTKDMETIKIKQREHGGWNANMNECLTGPCQLVQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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