60S ribosomal protein L8

Overview
Name60S ribosomal protein L8
Smed IDSMED30004484
Length (bp)854
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 60S ribosomal protein L8 (SMED30004484) t-SNE clustered cells

Violin plots show distribution of expression levels for 60S ribosomal protein L8 (SMED30004484) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 60S ribosomal protein L8 (SMED30004484) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 60S ribosomal protein L8 (SMED30004484) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30004484

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30004484h1SMcG0020811 SmedASXL_018659SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30004484h1SMcG0020811 SmedASXL_018659SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30004484h1SMcG0020811 dd_Smed_v4_108_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30004484h1SMcG0020811 dd_Smed_v6_108_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
progenitor cellSMED30004484h1SMcG0020811 dd_Smed_v6_108_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004484h1SMcG0020811 dd_Smed_v6_108_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 60S ribosomal protein L8 vs. Ensembl Human
Match: RPL8 (ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368])

HSP 1 Score: 375.941 bits (964), Expect = 4.142e-132
Identity = 186/254 (73.23%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVF+AH KHRKGAA+LRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Human
Match: RPL8 (ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368])

HSP 1 Score: 375.941 bits (964), Expect = 4.142e-132
Identity = 186/254 (73.23%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVF+AH KHRKGAA+LRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Human
Match: RPL8 (ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368])

HSP 1 Score: 375.941 bits (964), Expect = 4.142e-132
Identity = 186/254 (73.23%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVF+AH KHRKGAA+LRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Human
Match: RPL8 (ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368])

HSP 1 Score: 333.569 bits (854), Expect = 1.353e-115
Identity = 171/254 (67.32%), Postives = 205/254 (80.71%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVF+AH KHRKGAA+LRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A ++                     SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVI---------------------SSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 233          
BLAST of 60S ribosomal protein L8 vs. Ensembl Human
Match: RPL8 (ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:10368])

HSP 1 Score: 305.834 bits (782), Expect = 3.690e-105
Identity = 152/205 (74.15%), Postives = 184/205 (89.76%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMN 668
            MGRVI  QRKG GSVF+AH KHRKGAA+LRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MN
Sbjct:    1 MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMN 205          
BLAST of 60S ribosomal protein L8 vs. Ensembl Celegans
Match: rpl-2 (60S ribosomal protein L8 [Source:UniProtKB/Swiss-Prot;Acc:Q9XVF7])

HSP 1 Score: 373.244 bits (957), Expect = 2.277e-131
Identity = 174/257 (67.70%), Postives = 214/257 (83.27%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKNTKQ 824
            MGR I  QRKG G +FK+H KHRKGA+KLR  DYAER+G++KG+V+DIIHDPGRGAPLA + F+D YKYK +  T +AAEGM TGQF++CG KA+I +GN++P+ ++PEGT +CN+E KSGDRG IARASGN A ++AHN +T KTR++LPSG KK++ S  RAMIG+VAGGGR +KP+LKAGR+YHKYKAKRN WPRVRGV MNPV+HPHGGGNHQHIG PSTV R    G+KVGL+AARRTG +RG K +K TK+
Sbjct:    1 MGRRIRIQRKGAGGIFKSHNKHRKGASKLRPLDYAERHGYIKGLVKDIIHDPGRGAPLAIIAFRDPYKYKTVKTTVVAAEGMHTGQFIHCGAKAQIQIGNIVPVGTLPEGTTICNVENKSGDRGVIARASGNYATVIAHNPDTKKTRIRLPSGAKKVVQSVNRAMIGLVAGGGRTDKPLLKAGRSYHKYKAKRNSWPRVRGVAMNPVEHPHGGGNHQHIGHPSTVRRDASAGKKVGLIAARRTGRIRGGKPVKFTKE 257          
BLAST of 60S ribosomal protein L8 vs. Ensembl Fly
Match: RpL8 (gene:FBgn0261602 transcript:FBtr0072924)

HSP 1 Score: 377.867 bits (969), Expect = 3.359e-133
Identity = 178/250 (71.20%), Postives = 212/250 (84.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK 803
            MGRVI  QRKG GSVFKAH K RKGAAKLR+ D+AER+G+++G+V+DIIHDPGRGAPLA V F+D Y+YK   + FIA EGM TGQFVYCG+KA + +GNV+P+  +PEGT++CNLEEK+GDRGR+AR SGN A ++AHN +T KTRVKLPSG KK++ S+ RAM+GIVAGGGRI+KP+LKAGRAYHKYK KRN WP+VRGV MNPV+HPHGGGNHQHIG  STV R T  GRKVGL+AARRTG +RG K
Sbjct:    1 MGRVIRAQRKGAGSVFKAHVKKRKGAAKLRSLDFAERSGYIRGVVKDIIHDPGRGAPLAVVHFRDPYRYKIRKELFIAPEGMHTGQFVYCGRKATLQIGNVMPLSQMPEGTIICNLEEKTGDRGRLARTSGNYATVIAHNQDTKKTRVKLPSGAKKVVPSANRAMVGIVAGGGRIDKPILKAGRAYHKYKVKRNSWPKVRGVAMNPVEHPHGGGNHQHIGKASTVKRGTSAGRKVGLIAARRTGRIRGGK 250          
BLAST of 60S ribosomal protein L8 vs. Ensembl Fly
Match: RpL8 (gene:FBgn0261602 transcript:FBtr0072925)

HSP 1 Score: 377.867 bits (969), Expect = 3.359e-133
Identity = 178/250 (71.20%), Postives = 212/250 (84.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK 803
            MGRVI  QRKG GSVFKAH K RKGAAKLR+ D+AER+G+++G+V+DIIHDPGRGAPLA V F+D Y+YK   + FIA EGM TGQFVYCG+KA + +GNV+P+  +PEGT++CNLEEK+GDRGR+AR SGN A ++AHN +T KTRVKLPSG KK++ S+ RAM+GIVAGGGRI+KP+LKAGRAYHKYK KRN WP+VRGV MNPV+HPHGGGNHQHIG  STV R T  GRKVGL+AARRTG +RG K
Sbjct:    1 MGRVIRAQRKGAGSVFKAHVKKRKGAAKLRSLDFAERSGYIRGVVKDIIHDPGRGAPLAVVHFRDPYRYKIRKELFIAPEGMHTGQFVYCGRKATLQIGNVMPLSQMPEGTIICNLEEKTGDRGRLARTSGNYATVIAHNQDTKKTRVKLPSGAKKVVPSANRAMVGIVAGGGRIDKPILKAGRAYHKYKVKRNSWPKVRGVAMNPVEHPHGGGNHQHIGKASTVKRGTSAGRKVGLIAARRTGRIRGGK 250          
BLAST of 60S ribosomal protein L8 vs. Ensembl Zebrafish
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:393686])

HSP 1 Score: 374.4 bits (960), Expect = 1.047e-131
Identity = 185/254 (72.83%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVFKAH KHRKGAAKLR  D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQF+YCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET K+RVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK +++
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRHIDFAERHGYIKGIVKDIIHDPGRGAPLAKVMFRDPYRFKKRTELFIAAEGIHTGQFIYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKSRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQD 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Zebrafish
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:393686])

HSP 1 Score: 374.4 bits (960), Expect = 1.047e-131
Identity = 185/254 (72.83%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVFKAH KHRKGAAKLR  D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQF+YCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET K+RVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK +++
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRHIDFAERHGYIKGIVKDIIHDPGRGAPLAKVMFRDPYRFKKRTELFIAAEGIHTGQFIYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKSRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQD 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Zebrafish
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:393686])

HSP 1 Score: 194.897 bits (494), Expect = 8.048e-63
Identity = 91/124 (73.39%), Postives = 109/124 (87.90%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRG 425
            MGRVI  QRKG GSVFKAH KHRKGAAKLR  D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQF+YCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRHIDFAERHGYIKGIVKDIIHDPGRGAPLAKVMFRDPYRFKKRTELFIAAEGIHTGQFIYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRG 124          
BLAST of 60S ribosomal protein L8 vs. Ensembl Xenopus
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:394521])

HSP 1 Score: 396.356 bits (1017), Expect = 3.766e-140
Identity = 186/254 (73.23%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVFKAH KHRKGAAKLRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + F+AAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC +EEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVAFRDPYRFKKRTELFVAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCVEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAIVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Xenopus
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:394521])

HSP 1 Score: 162.54 bits (410), Expect = 1.170e-49
Identity = 75/100 (75.00%), Postives = 88/100 (88.00%), Query Frame = 3
Query:  516 KKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:   46 KKVISSANRAIVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 145          

HSP 2 Score: 76.6406 bits (187), Expect = 1.008e-16
Identity = 38/54 (70.37%), Postives = 44/54 (81.48%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGR 215
            MGRVI  QRKG GSVFKAH KHRKGAAKLRA D+AER+G++KGIV+ +I    R
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRAVDFAERHGYIKGIVKKVISSANR 54          
BLAST of 60S ribosomal protein L8 vs. Ensembl Mouse
Match: Rpl8 (ribosomal protein L8 [Source:MGI Symbol;Acc:MGI:1350927])

HSP 1 Score: 375.941 bits (964), Expect = 2.843e-132
Identity = 186/254 (73.23%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVF+AH KHRKGAA+LRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Mouse
Match: Rpl8 (ribosomal protein L8 [Source:MGI Symbol;Acc:MGI:1350927])

HSP 1 Score: 375.941 bits (964), Expect = 2.843e-132
Identity = 186/254 (73.23%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVF+AH KHRKGAA+LRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. UniProt/SwissProt
Match: sp|P41116|RL8_XENLA (60S ribosomal protein L8 OS=Xenopus laevis OX=8355 GN=rpl8 PE=2 SV=3)

HSP 1 Score: 398.282 bits (1022), Expect = 2.903e-140
Identity = 187/254 (73.62%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVFKAH KHRKGAAKLRA D+AERNG++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + F+AAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC +EEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRAIDFAERNGYIKGIVKDIIHDPGRGAPLAKVAFRDPYRFKKRTELFVAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCVEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAIVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. UniProt/SwissProt
Match: sp|Q6PBF0|RL8_XENTR (60S ribosomal protein L8 OS=Xenopus tropicalis OX=8364 GN=rpl8 PE=2 SV=3)

HSP 1 Score: 396.356 bits (1017), Expect = 1.995e-139
Identity = 186/254 (73.23%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVFKAH KHRKGAAKLRA D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + F+AAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC +EEK GDRG++ARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVAFRDPYRFKKRTELFVAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCVEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAIVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. UniProt/SwissProt
Match: sp|Q9U9L2|RL8_ANOGA (60S ribosomal protein L8 OS=Anopheles gambiae OX=7165 GN=RpL8 PE=2 SV=2)

HSP 1 Score: 379.793 bits (974), Expect = 8.214e-133
Identity = 182/250 (72.80%), Postives = 213/250 (85.20%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK 803
            MGRVI  QRKG GSVF+AHTK RKG  KLR  DYAER+G+LKG+V+ IIHDPGRGAPLA V F+D Y+++   Q FIAAEGM TGQFVYCG++A++ +GNVLPI  +PEGT+VCNLEEK+GDRG++AR SGN A ++AHN +T +TRVKLPSG KK+L S+ RAMIGIVAGGGRI+KP+LKAGRAYHKYK KRNCWP+VRGV MNPV+HPHGGGNHQHIG  STV R T PGRKVGL+AARRTG +RG K
Sbjct:    1 MGRVIRAQRKGAGSVFRAHTKKRKGEPKLRHLDYAERHGYLKGVVKQIIHDPGRGAPLAVVNFRDPYRFRLSKQLFIAAEGMYTGQFVYCGRRAQLQIGNVLPIGLMPEGTIVCNLEEKTGDRGKLARTSGNYASVIAHNPDTKRTRVKLPSGAKKVLPSANRAMIGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPKVRGVAMNPVEHPHGGGNHQHIGKASTVKRGTPPGRKVGLIAARRTGRIRGGK 250          
BLAST of 60S ribosomal protein L8 vs. UniProt/SwissProt
Match: sp|Q9V3G1|RL8_DROME (60S ribosomal protein L8 OS=Drosophila melanogaster OX=7227 GN=RpL8 PE=1 SV=1)

HSP 1 Score: 377.867 bits (969), Expect = 3.367e-132
Identity = 178/250 (71.20%), Postives = 212/250 (84.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK 803
            MGRVI  QRKG GSVFKAH K RKGAAKLR+ D+AER+G+++G+V+DIIHDPGRGAPLA V F+D Y+YK   + FIA EGM TGQFVYCG+KA + +GNV+P+  +PEGT++CNLEEK+GDRGR+AR SGN A ++AHN +T KTRVKLPSG KK++ S+ RAM+GIVAGGGRI+KP+LKAGRAYHKYK KRN WP+VRGV MNPV+HPHGGGNHQHIG  STV R T  GRKVGL+AARRTG +RG K
Sbjct:    1 MGRVIRAQRKGAGSVFKAHVKKRKGAAKLRSLDFAERSGYIRGVVKDIIHDPGRGAPLAVVHFRDPYRYKIRKELFIAPEGMHTGQFVYCGRKATLQIGNVMPLSQMPEGTIICNLEEKTGDRGRLARTSGNYATVIAHNQDTKKTRVKLPSGAKKVVPSANRAMVGIVAGGGRIDKPILKAGRAYHKYKVKRNSWPKVRGVAMNPVEHPHGGGNHQHIGKASTVKRGTSAGRKVGLIAARRTGRIRGGK 250          
BLAST of 60S ribosomal protein L8 vs. UniProt/SwissProt
Match: sp|P41569|RL8_AEDAL (60S ribosomal protein L8 OS=Aedes albopictus OX=7160 GN=RpL8 PE=3 SV=1)

HSP 1 Score: 377.481 bits (968), Expect = 5.579e-132
Identity = 183/250 (73.20%), Postives = 210/250 (84.00%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK 803
            MGRVI  QRKG GSVF AHTK RKG  KLRA DYAER+G+LKG V+ IIHDPGRGAPLA V F+D YK+    Q FIAAEGM TGQFVYCGK+A++ +GNVLPI  +PEGT+VCNLEEK+GDRG++AR SGN A ++AHN +T +TRVKLPSG KK+L S+ RAM+GIVAGGGRI+KP+LKAGRAY KYK KRNCWP+VRGV MNPV+HPHGGGNHQHIG  STV R T PGRKVGL+AARRTG +RG K
Sbjct:    1 MGRVIRAQRKGEGSVFTAHTKKRKGEPKLRALDYAERHGYLKGTVKQIIHDPGRGAPLAVVHFRDPYKFNVRKQLFIAAEGMYTGQFVYCGKRAQLQIGNVLPIGLMPEGTIVCNLEEKTGDRGKLARTSGNYASVIAHNPDTKRTRVKLPSGAKKVLPSANRAMVGIVAGGGRIDKPILKAGRAYRKYKVKRNCWPKVRGVAMNPVEHPHGGGNHQHIGKASTVKRGTPPGRKVGLIAARRTGRIRGGK 250          
BLAST of 60S ribosomal protein L8 vs. TrEMBL
Match: A0A1I8I4N7 (Ribosomal_L2_C domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013601g1 PE=4 SV=1)

HSP 1 Score: 401.364 bits (1030), Expect = 7.521e-139
Identity = 183/254 (72.05%), Postives = 226/254 (88.98%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI +QRKG GSVF++HTK RKGAA+LR  D+AERNG++KG+VRDIIHDPGRGAPLAKV F+D Y+Y+++ +TF+AAEG+ TGQF+YCGKKAK+ VGN+LP+ ++PEGT++C +EEK+GDRGR+A+ASGN A +VAHN ET ++R+KLPSG KK++ S+CRAM+GI+AGGGRI+KP++KAGRAY KYK KRNCWP+VRGVCMNPVDHP GGGNHQHIG PST+ R T  GRKVGL+AARRTG LRGTKVI N
Sbjct:    1 MGRVIRSQRKGAGSVFRSHTKRRKGAARLRPIDFAERNGYIKGVVRDIIHDPGRGAPLAKVAFRDPYRYRQVTETFVAAEGVHTGQFIYCGKKAKLQVGNILPLGAMPEGTIICCIEEKAGDRGRLAKASGNYATLVAHNPETKRSRMKLPSGAKKIVPSACRAMVGIIAGGGRIDKPIMKAGRAYWKYKVKRNCWPKVRGVCMNPVDHPFGGGNHQHIGKPSTIRRDTSAGRKVGLIAARRTGRLRGTKVIVN 254          
BLAST of 60S ribosomal protein L8 vs. TrEMBL
Match: B6RB82 (Ribosomal protein l OS=Haliotis discus discus OX=91233 PE=2 SV=1)

HSP 1 Score: 400.593 bits (1028), Expect = 1.259e-138
Identity = 191/252 (75.79%), Postives = 221/252 (87.70%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVI 809
            MGRVI +QRKG GSVFKAHTKHRKGAAKLRA DYAER+G++KGI++DIIHDPGRGAPL KV F+D Y+YK   +TFIAAEGM TGQFVYCGKKA + VGNVLP+ S+PEGT +C+LEEK+GDRG++ARASGN A +++HN +T +TRVKLPSG KK + S+ RAM+GIVAGGGRI+KPMLKAGRAYHKYKAKRNCWP+VRGV MNPV+HPHGGGNHQHIG  STV R T  GRKVGL+AARRTG LRGTK +
Sbjct:    1 MGRVIRSQRKGPGSVFKAHTKHRKGAAKLRAVDYAERHGYIKGIIKDIIHDPGRGAPLCKVVFRDPYRYKMKNETFIAAEGMYTGQFVYCGKKANLQVGNVLPVGSMPEGTTICSLEEKTGDRGKLARASGNYATVISHNPDTRRTRVKLPSGSKKAIPSTNRAMVGIVAGGGRIDKPMLKAGRAYHKYKAKRNCWPKVRGVAMNPVEHPHGGGNHQHIGKASTVRRDTSAGRKVGLIAARRTGRLRGTKQL 252          
BLAST of 60S ribosomal protein L8 vs. TrEMBL
Match: A0A2H9TJB0 (Ribosomal_L2_C domain-containing protein OS=Paramicrosporidium saccamoebae OX=1246581 GN=PSACC_02363 PE=4 SV=1)

HSP 1 Score: 400.208 bits (1027), Expect = 2.005e-138
Identity = 180/255 (70.59%), Postives = 222/255 (87.06%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKNT 818
            MGRVI  QRKG G +FKAHTKHR+GAA  R  D+AER G+++G+VR+I+HDPGRGAPLAKVEF+D Y+Y+K+++TF+A EGM TGQFVYCGKKA ++VGN+LP+QS+PEGT+VCN+EEK+GD G +AR SGN A ++ HN + G TR+K+PSG KKL+S  CRAMIG+VAGGGRI+KP+LKAGRAY+KYKAKRNCWP+VRGV MNPVDHPHGGGNHQH+G  +TVSR   PG+KVGL+AARRTGLLRGTK ++ T
Sbjct:    1 MGRVIRTQRKGHGGIFKAHTKHRQGAAAFRRLDFAEREGYIRGVVREIVHDPGRGAPLAKVEFRDPYRYRKVVETFVAIEGMYTGQFVYCGKKATLSVGNILPLQSMPEGTIVCNVEEKAGDGGSVARCSGNYATVIGHNVDDGTTRIKMPSGSKKLVSGRCRAMIGLVAGGGRIDKPLLKAGRAYYKYKAKRNCWPKVRGVAMNPVDHPHGGGNHQHVGHATTVSRFAPPGQKVGLIAARRTGLLRGTKKVRET 255          
BLAST of 60S ribosomal protein L8 vs. TrEMBL
Match: A0A1L8HF43 (Ribosomal_L2_C domain-containing protein OS=Xenopus laevis OX=8355 GN=XELAEV_18012399mg PE=3 SV=1)

HSP 1 Score: 399.438 bits (1025), Expect = 3.742e-138
Identity = 188/254 (74.02%), Postives = 223/254 (87.80%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVFKAH KHRKGAAKLRA D+AERNG++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + F+AAEG+ TGQFVYCGKKA++N+GNVLP+ ++PEGT+VC +EEK GDRG+IARASGN A +++HN ET KTRVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRAIDFAERNGYIKGIVKDIIHDPGRGAPLAKVAFRDPYRFKKRTELFVAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCVEEKPGDRGKIARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAIVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. TrEMBL
Match: A0A4D8Y4P8 (Ribosomal protein L2 OS=Perkinsus sp. BL_2016 OX=2494336 GN=C9890_0659 PE=3 SV=1)

HSP 1 Score: 399.053 bits (1024), Expect = 4.723e-138
Identity = 186/255 (72.94%), Postives = 219/255 (85.88%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKNT 818
            MGRVI  QRKG G +FKAHTKHRKGAA+LRA DYAER+G+++G+V++IIHDPGRGAPLAKVEF+DAYKYK    TFIA EGM TGQFVYCG KA + +GNV+P+  +PEGT++CN+EEK GDRG +ARASGN A ++ HN +   TR+KLPSG KKL+SS CRA +GIVAGGGRI+KP++KAGRAY+KYKAKRNCWP+VRGV MNPVDHPHGGGNHQHIG  STVSR   PG+KVGLVAARRTGLLRGTK ++ T
Sbjct:    1 MGRVIRAQRKGHGGIFKAHTKHRKGAAELRALDYAERHGYIRGLVKEIIHDPGRGAPLAKVEFRDAYKYKMHTSTFIATEGMYTGQFVYCGAKATLTIGNVMPVGQMPEGTIICNVEEKVGDRGAVARASGNYATVIGHNVDEKVTRIKLPSGAKKLVSSDCRATVGIVAGGGRIDKPIMKAGRAYYKYKAKRNCWPKVRGVAMNPVDHPHGGGNHQHIGHASTVSRFAPPGQKVGLVAARRTGLLRGTKKVRET 255          
BLAST of 60S ribosomal protein L8 vs. Ensembl Cavefish
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:103039806])

HSP 1 Score: 384.8 bits (987), Expect = 7.972e-136
Identity = 181/257 (70.43%), Postives = 222/257 (86.38%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKNTKQ 824
            MGRVI  QRKG GSVFKAH K RKGAAKLR  D+AER+G++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQF++CGK+A++N+GNV+P+ ++PEGT+VC LEEK GDRG++ARASGN A +++HN ET K+RVKLPSG KK++SS+ RA++GIVAGGGRI+KP+LKAGRAYHKYK KRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK +++ + 
Sbjct:    1 MGRVIRAQRKGAGSVFKAHVKLRKGAAKLRHIDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFIFCGKRAQLNIGNVMPVGNMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKSRVKLPSGSKKVVSSANRAVVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQDKEN 257          
BLAST of 60S ribosomal protein L8 vs. Ensembl Cavefish
Match: mrpl2 (mitochondrial ribosomal protein L2 [Source:NCBI gene;Acc:103037714])

HSP 1 Score: 48.521 bits (114), Expect = 3.050e-6
Identity = 44/162 (27.16%), Postives = 78/162 (48.15%), Query Frame = 3
Query:  135 KLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFV----YCGKKAKI-NVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGR 605
            +LR E   E    ++ +V ++ +DP R A +A V   +  ++       IA E M  G  +      G+ A + N G+  P+ ++P GT++ NLE   G   +  RA+G   +++      G   V+LPS  +  +  +C A +G V+     ++ + KAGR
Sbjct:  110 RLRYEAGKEGEQIVEKVV-EVRYDPCRSADIALVAGGNRKRW------IIATENMQAGDLIKTSSVIGRMAVLANEGDAHPLGALPVGTLINNLELYPGKGAQYIRAAGTCGVLL--RKVNGTAIVQLPSKHQIQVLETCVATVGKVSNIDHNKRVIGKAGR 262          
BLAST of 60S ribosomal protein L8 vs. Ensembl Sea Lamprey
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:393686])

HSP 1 Score: 274.633 bits (701), Expect = 6.602e-94
Identity = 136/190 (71.58%), Postives = 167/190 (87.89%), Query Frame = 3
Query:  240 FKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVI 809
            F+D Y++KK  + FIAAEG+ TGQFVYCGKKA+++VGNVLP+  +PEGT+VC +EEKSGDRG++ARASGN A +++HN +T K+RVKLPSG KK++SS+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTKV+
Sbjct:    2 FRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLSVGNVLPVGVMPEGTIVCCVEEKSGDRGKLARASGNYATVISHNPDTHKSRVKLPSGAKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKVV 191          
BLAST of 60S ribosomal protein L8 vs. Ensembl Sea Lamprey
Match: mrpl2 (mitochondrial ribosomal protein L2 [Source:ZFIN;Acc:ZDB-GENE-050809-12])

HSP 1 Score: 53.1434 bits (126), Expect = 1.668e-8
Identity = 42/161 (26.09%), Postives = 79/161 (49.07%), Query Frame = 3
Query:  138 LRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFV----YCGKKAKI-NVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGR 605
            L A    E    ++  V ++ +DP R A +A V      ++       IA + M  G  +    + G+ A + + G+  P+ ++P GT++ NLE K G   ++ RA+G   +++      G   ++LPS  +  ++ +C A +G V+     ++P+ KAGR
Sbjct:  115 LMAGRLDENKKVMEEKVMEVRYDPLRSADIALVAGGTHKRW------IIATQSMQPGDVIKTSGFIGRMAVLAHEGDAYPVGALPVGTLINNLEVKPGAGAQLIRAAGTCGLLI--RKVEGTAIIQLPSKHQIQVAETCMATVGRVSRVEHNKEPIGKAGR 267          
BLAST of 60S ribosomal protein L8 vs. Ensembl Yeast
Match: RPL2A (Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication [Source:SGD;Acc:S000002104])

HSP 1 Score: 346.28 bits (887), Expect = 1.687e-121
Identity = 168/250 (67.20%), Postives = 208/250 (83.20%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK 803
            MGRVI NQRKG GS+F +HT+ R+GAAKLR  DYAER+G+++GIV+ I+HD GRGAPLAKV F+D YKY+   + FIA EG+ TGQF+Y GKKA +NVGNVLP+ S+PEGT+V N+EEK GDRG +ARASGN  II+ HN +  KTRV+LPSG KK++SS  R +IG++AGGGR++KP+LKAGRA+HKY+ KRN WP+ RGV MNPVDHPHGGGNHQHIG  ST+SR  V G+K GL+AARRTGLLRG++
Sbjct:    1 MGRVIRNQRKGAGSIFTSHTRLRQGAAKLRTLDYAERHGYIRGIVKQIVHDSGRGAPLAKVVFRDPYKYRLREEIFIANEGVHTGQFIYAGKKASLNVGNVLPLGSVPEGTIVSNVEEKPGDRGALARASGNYVIIIGHNPDENKTRVRLPSGAKKVISSDARGVIGVIAGGGRVDKPLLKAGRAFHKYRLKRNSWPKTRGVAMNPVDHPHGGGNHQHIGKASTISRGAVSGQKAGLIAARRTGLLRGSQ 250          
BLAST of 60S ribosomal protein L8 vs. Ensembl Yeast
Match: RPL2B (Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures [Source:SGD;Acc:S000001280])

HSP 1 Score: 346.28 bits (887), Expect = 1.687e-121
Identity = 168/250 (67.20%), Postives = 208/250 (83.20%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK 803
            MGRVI NQRKG GS+F +HT+ R+GAAKLR  DYAER+G+++GIV+ I+HD GRGAPLAKV F+D YKY+   + FIA EG+ TGQF+Y GKKA +NVGNVLP+ S+PEGT+V N+EEK GDRG +ARASGN  II+ HN +  KTRV+LPSG KK++SS  R +IG++AGGGR++KP+LKAGRA+HKY+ KRN WP+ RGV MNPVDHPHGGGNHQHIG  ST+SR  V G+K GL+AARRTGLLRG++
Sbjct:    1 MGRVIRNQRKGAGSIFTSHTRLRQGAAKLRTLDYAERHGYIRGIVKQIVHDSGRGAPLAKVVFRDPYKYRLREEIFIANEGVHTGQFIYAGKKASLNVGNVLPLGSVPEGTIVSNVEEKPGDRGALARASGNYVIIIGHNPDENKTRVRLPSGAKKVISSDARGVIGVIAGGGRVDKPLLKAGRAFHKYRLKRNSWPKTRGVAMNPVDHPHGGGNHQHIGKASTISRGAVSGQKAGLIAARRTGLLRGSQ 250          
BLAST of 60S ribosomal protein L8 vs. Ensembl Yeast
Match: RML2 (Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant allele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor [Source:SGD;Acc:S000000776])

HSP 1 Score: 73.9442 bits (180), Expect = 1.530e-15
Identity = 68/234 (29.06%), Postives = 109/234 (46.58%), Query Frame = 3
Query:   63 VILNQRKGRGSVFKAHTKHRKGAAKLRAE--DYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKK----------------AKINV-----GNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGG 695
            V+  +  GR +  K   +H+ G  + R    D+    G  + + R I +DPGR + +A ++     +    +   IA +G+  G  V   ++                A ++V     GN LPI  IP GT++ N+       G+  R++G  A ++A   E  K  V+L SG+ + +S    A IG+V+      + + KAGR+  ++   R   P VRGV MN  DHPHGGG
Sbjct:  125 VVRKKHGGRNNSGKITVRHQGGGHRNRTRLIDFNRWEGGAQTVQR-IEYDPGRSSHIALLKHNTTGE----LSYIIACDGLRPGDVVESFRRGIPQTLLNEMGGKVDPAILSVKTTQRGNCLPISMIPIGTIIHNVGITPVGPGKFCRSAGTYARVLAKLPEKKKAIVRLQSGEHRYVSLEAVATIGVVSNIDHQNRSLGKAGRS--RWLGIR---PTVRGVAMNKCDHPHGGG 348          
BLAST of 60S ribosomal protein L8 vs. Ensembl Nematostella
Match: EDO40963 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5T9])

HSP 1 Score: 380.948 bits (977), Expect = 9.432e-135
Identity = 180/258 (69.77%), Postives = 217/258 (84.11%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLR-GTKVIKNTKQ 824
            MGRVI  QRKG GS+F +HTKHRKGAA LR  DYAER+G++KG+V++IIHDPGRGAPLA V F+D Y+YK   + F+A EGM TGQF+YCGKKA + +GN LP+  +PEGT++ ++EEK+GDRGR+AR SGN A +++HN E  +TRVKLPSG KK++ SS RA++GIVAGGGRI+KPMLKAGRAYHKYKAKRNCWPRVRGV MNPV+HPHGGGNHQHIG PSTV R T  GRKVGL+AARRTGLLR G K++K  K+
Sbjct:    1 MGRVIRGQRKGAGSIFTSHTKHRKGAANLRVHDYAERHGYIKGVVKEIIHDPGRGAPLAVVVFRDPYRYKLRKELFVATEGMYTGQFIYCGKKAALQIGNCLPVGIMPEGTIISSVEEKTGDRGRLARTSGNYATVISHNVEKKRTRVKLPSGIKKVIPSSNRALVGIVAGGGRIDKPMLKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPHGGGNHQHIGHPSTVRRDTPAGRKVGLIAARRTGLLRGGQKILKGDKE 258          
BLAST of 60S ribosomal protein L8 vs. Ensembl Nematostella
Match: EDO48947 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI04])

HSP 1 Score: 55.4546 bits (132), Expect = 2.094e-9
Identity = 47/149 (31.54%), Postives = 75/149 (50.34%), Query Frame = 3
Query:  174 LKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGK-----KAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGR 605
            +K  V  + +DP R A +A V    + + K +    IA + +  G  +   +      A +  G+  P+  +P GTVV N+E   G   ++ARA+G SA ++    ET    V+LPS  +K +SS C A +G V+   R  + + KAGR
Sbjct:   41 IKDKVLQVGYDPCRSARIALVAGNGSNQEKLV----IAPDEIQVGDVMTASRGKPESLALLKPGDAYPLCFLPIGTVVHNIELYPGKGAQLARAAGTSAQLIRKTNETAV--VRLPSKVEKEVSSKCLASVGRVSNIERKNRVIGKAGR 183          
BLAST of 60S ribosomal protein L8 vs. Ensembl Medaka
Match: rpl8 (ribosomal protein L8 [Source:NCBI gene;Acc:100125472])

HSP 1 Score: 374.015 bits (959), Expect = 1.381e-131
Identity = 184/254 (72.44%), Postives = 222/254 (87.40%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKN 815
            MGRVI  QRKG GSVFKAH KHRKGAAKLR  D+AERNG++KGIV+DIIHDPGRGAPLAKV F+D Y++KK  + FIAAEG+ TGQF+YCGKKA++N+GNVLP+ ++PEGT++C LEEK GDRG++ARASGN A +++HN ET K+RVKLPSG KK+++S+ RA++G+VAGGGRI+KP+LKAGRAYHKYKAKRNCWPRVRGV MNPV+HP GGGNHQHIG PST+ R    GRKVGL+AARRTG LRGTK ++ 
Sbjct:    1 MGRVIRGQRKGAGSVFKAHVKHRKGAAKLRHIDFAERNGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFIYCGKKAQLNIGNVLPVGTMPEGTIICCLEEKPGDRGKLARASGNYATVISHNPETKKSRVKLPSGAKKVIASANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQE 254          
BLAST of 60S ribosomal protein L8 vs. Ensembl Medaka
Match: mrpl2 (mitochondrial ribosomal protein L2 [Source:NCBI gene;Acc:101171275])

HSP 1 Score: 51.6026 bits (122), Expect = 3.016e-7
Identity = 42/145 (28.97%), Postives = 70/145 (48.28%), Query Frame = 3
Query:  186 VRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFV----YCGKKA-KINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGR 605
            V ++ +DP R A +A V   +  ++       IA E M  G  +      G+ A   N G+  P+ ++P GT+V NLE + G      RA+G S +++      G   V+LPS Q+  +  +C   +G V+     ++ + KAGR
Sbjct:  119 VVEVRYDPCRSADIALVAGGNRKRW------IIATETMQAGNIIKTSGVIGRMAVSANEGDAYPLGALPVGTLVNNLEVQPGRGAEYIRAAGTSGVLL--RKVNGTAIVQLPSKQQVQVLETCMVTVGRVSNVDHNKRVIGKAGR 255          
BLAST of 60S ribosomal protein L8 vs. Planmine SMEST
Match: SMESG000017087.1 (SMESG000017087.1)

HSP 1 Score: 524.628 bits (1350), Expect = 0.000e+0
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 3
Query:   54 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKNTKQ 824
            MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKNTKQ
Sbjct:    1 MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIHDPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGNVLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKLPSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVRGVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTKVIKNTKQ 257          
The following BLAST results are available for this feature:
BLAST of 60S ribosomal protein L8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPL84.142e-13273.23ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPL84.142e-13273.23ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPL84.142e-13273.23ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPL81.353e-11567.32ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:... [more]
RPL83.690e-10574.15ribosomal protein L8 [Source:HGNC Symbol;Acc:HGNC:... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpl-22.277e-13167.7060S ribosomal protein L8 [Source:UniProtKB/Swiss-... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
RpL83.359e-13371.20gene:FBgn0261602 transcript:FBtr0072924[more]
RpL83.359e-13371.20gene:FBgn0261602 transcript:FBtr0072925[more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpl81.047e-13172.83ribosomal protein L8 [Source:NCBI gene;Acc:393686][more]
rpl81.047e-13172.83ribosomal protein L8 [Source:NCBI gene;Acc:393686][more]
rpl88.048e-6373.39ribosomal protein L8 [Source:NCBI gene;Acc:393686][more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl83.766e-14073.23ribosomal protein L8 [Source:NCBI gene;Acc:394521][more]
rpl81.170e-4975.00ribosomal protein L8 [Source:NCBI gene;Acc:394521][more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rpl82.843e-13273.23ribosomal protein L8 [Source:MGI Symbol;Acc:MGI:13... [more]
Rpl82.843e-13273.23ribosomal protein L8 [Source:MGI Symbol;Acc:MGI:13... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P41116|RL8_XENLA2.903e-14073.6260S ribosomal protein L8 OS=Xenopus laevis OX=8355... [more]
sp|Q6PBF0|RL8_XENTR1.995e-13973.2360S ribosomal protein L8 OS=Xenopus tropicalis OX=... [more]
sp|Q9U9L2|RL8_ANOGA8.214e-13372.8060S ribosomal protein L8 OS=Anopheles gambiae OX=7... [more]
sp|Q9V3G1|RL8_DROME3.367e-13271.2060S ribosomal protein L8 OS=Drosophila melanogaste... [more]
sp|P41569|RL8_AEDAL5.579e-13273.2060S ribosomal protein L8 OS=Aedes albopictus OX=71... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8I4N77.521e-13972.05Ribosomal_L2_C domain-containing protein OS=Macros... [more]
B6RB821.259e-13875.79Ribosomal protein l OS=Haliotis discus discus OX=9... [more]
A0A2H9TJB02.005e-13870.59Ribosomal_L2_C domain-containing protein OS=Parami... [more]
A0A1L8HF433.742e-13874.02Ribosomal_L2_C domain-containing protein OS=Xenopu... [more]
A0A4D8Y4P84.723e-13872.94Ribosomal protein L2 OS=Perkinsus sp. BL_2016 OX=2... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl87.972e-13670.43ribosomal protein L8 [Source:NCBI gene;Acc:1030398... [more]
mrpl23.050e-627.16mitochondrial ribosomal protein L2 [Source:NCBI ge... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl86.602e-9471.58ribosomal protein L8 [Source:NCBI gene;Acc:393686][more]
mrpl21.668e-826.09mitochondrial ribosomal protein L2 [Source:ZFIN;Ac... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
RPL2A1.687e-12167.20Ribosomal 60S subunit protein L2A; homologous to m... [more]
RPL2B1.687e-12167.20Ribosomal 60S subunit protein L2B; homologous to m... [more]
RML21.530e-1529.06Mitochondrial ribosomal protein of the large subun... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO409639.432e-13569.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO489472.094e-931.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rpl81.381e-13172.44ribosomal protein L8 [Source:NCBI gene;Acc:1001254... [more]
mrpl23.016e-728.97mitochondrial ribosomal protein L2 [Source:NCBI ge... [more]
back to top
BLAST of 60S ribosomal protein L8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000017087.10.000e+0100.00SMESG000017087.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004484 ID=SMED30004484|Name=60S ribosomal protein L8|organism=Schmidtea mediterranea sexual|type=transcript|length=854bp
AAAGTTGTTTAAATTACTGATTCATATTAAAAAAGAATAATTTAATTGTA
AAGATGGGTCGAGTTATTTTGAATCAAAGAAAAGGTAGAGGATCAGTTTT
CAAGGCACATACCAAGCATCGTAAAGGAGCAGCTAAATTGAGAGCTGAAG
ATTATGCTGAAAGAAATGGATTTTTGAAAGGTATTGTTAGAGATATTATT
CATGATCCAGGAAGAGGAGCCCCATTGGCTAAAGTTGAATTCAAAGACGC
TTATAAATACAAGAAAATAATGCAAACTTTTATTGCTGCCGAAGGAATGT
CAACTGGTCAATTTGTATATTGTGGAAAGAAAGCCAAGATTAATGTTGGT
AATGTTTTACCAATTCAATCAATCCCAGAAGGTACCGTTGTTTGCAATCT
TGAAGAGAAATCTGGAGATAGAGGACGTATTGCACGTGCCAGTGGAAATT
CAGCTATTATTGTAGCACACAATACTGAAACTGGTAAAACACGTGTCAAA
TTGCCAAGTGGTCAAAAGAAACTATTGTCATCTTCATGTCGTGCTATGAT
TGGTATTGTTGCTGGTGGTGGAAGAATTGAAAAACCAATGCTTAAAGCTG
GTCGTGCTTACCATAAATACAAGGCCAAGAGAAACTGCTGGCCAAGAGTT
AGAGGTGTCTGCATGAACCCAGTTGACCATCCTCACGGTGGTGGTAACCA
TCAACATATTGGTACCCCGTCAACTGTTTCAAGACACACTGTTCCAGGTA
GAAAGGTTGGTTTGGTTGCTGCCAGAAGAACCGGTTTATTGAGAGGAACC
AAAGTTATTAAAAATACCAAACAATAACTTTTGAAATATAAATTTCTGGA
TTTT
back to top

protein sequence of SMED30004484-orf-1

>SMED30004484-orf-1 ID=SMED30004484-orf-1|Name=SMED30004484-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=258bp
MGRVILNQRKGRGSVFKAHTKHRKGAAKLRAEDYAERNGFLKGIVRDIIH
DPGRGAPLAKVEFKDAYKYKKIMQTFIAAEGMSTGQFVYCGKKAKINVGN
VLPIQSIPEGTVVCNLEEKSGDRGRIARASGNSAIIVAHNTETGKTRVKL
PSGQKKLLSSSCRAMIGIVAGGGRIEKPMLKAGRAYHKYKAKRNCWPRVR
GVCMNPVDHPHGGGNHQHIGTPSTVSRHTVPGRKVGLVAARRTGLLRGTK
VIKNTKQ*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000120progenitor cell
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR002171Ribosomal_L2
IPR022669Ribosomal_L2_C
IPR014726Ribosomal_L2_dom3
IPR022666Rbsml_prot_L2_RNA-bd_dom
IPR012340NA-bd_OB-fold
IPR014722Rib_L2_dom2
IPR008991Translation_prot_SH3-like_sf
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
GO:0005737cytoplasm
GO:0015934large ribosomal subunit
GO:0030529intracellular ribonucleoprotein complex
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
GO:0003723RNA binding
GO:0019843rRNA binding
Vocabulary: biological process
TermDefinition
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002171Ribosomal protein L2SMARTSM01383Ribosomal_L2_2coord: 11..90
e-value: 4.8E-30
score: 115.8
IPR002171Ribosomal protein L2PIRSFPIRSF002158RPL2p_RPL2a_RPL8e_RPL2ocoord: 1..257
e-value: 3.0E-68
score: 227.7
IPR002171Ribosomal protein L2PANTHERPTHR13691RIBOSOMAL PROTEIN L2coord: 1..253
IPR022669Ribosomal protein L2, C-terminalSMARTSM01382Ribosomal_L2_C_2coord: 96..231
e-value: 1.2E-72
score: 257.4
IPR022669Ribosomal protein L2, C-terminalPFAMPF03947Ribosomal_L2_Ccoord: 97..223
e-value: 3.5E-47
score: 159.7
IPR022666Ribosomal Proteins L2, RNA binding domainPFAMPF00181Ribosomal_L2coord: 11..90
e-value: 3.1E-16
score: 59.1
IPR014726Ribosomal protein L2, domain 3GENE3DG3DSA:4.10.950.10coord: 170..247
e-value: 5.7E-43
score: 147.1
IPR014722Ribosomal protein L2, domain 2GENE3DG3DSA:2.30.30.30coord: 90..169
e-value: 2.8E-36
score: 125.1
NoneNo IPR availableGENE3DG3DSA:2.40.50.140coord: 1..86
e-value: 9.4E-40
score: 136.6
NoneNo IPR availablePANTHERPTHR13691:SF1660S RIBOSOMAL PROTEIN L8coord: 1..253
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILYSSF50249Nucleic acid-binding proteinscoord: 1..97
IPR008991Translation protein SH3-like domain superfamilySUPERFAMILYSSF50104Translation proteins SH3-like domaincoord: 99..245