Thymidylate synthase-like Protein

Overview
NameThymidylate synthase-like Protein
Smed IDSMED30004470
Length (bp)1810
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Thymidylate synthase-like Protein (SMED30004470) t-SNE clustered cells

Violin plots show distribution of expression levels for Thymidylate synthase-like Protein (SMED30004470) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Thymidylate synthase-like Protein (SMED30004470) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Thymidylate synthase-like Protein (SMED30004470) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004470

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30004470h1SMcG0017834 SmedASXL_004854SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30004470h1SMcG0017834 dd_1260dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Smed sexual biotypeSMED30004470h1SMcG0017834 Contig40031newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004470h1SMcG0017834 Contig40031uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30004470h1SMcG0017834 dd_Smed_v4_1260_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004470h1SMcG0017834 dd_Smed_v4_1260_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004470h1SMcG0017834 dd_Smed_v4_1260_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30004470h1SMcG0017834 dd_Smed_v4_1260_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Human
Match: TYMS (thymidylate synthetase [Source:HGNC Symbol;Acc:HGNC:12441])

HSP 1 Score: 378.637 bits (971), Expect = 1.556e-127
Identity = 186/291 (63.92%), Postives = 230/291 (79.04%), Query Frame = 3 
Query:  843 PVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            P   H E+QYL  +++IL  G +  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++GSTN  EL++K V IWDANGS +FL S+G   RE GDLGPVYGFQWRHFGA+Y  M +DYS QGVDQ+  VI+ IK NP  +  ++ AWNP+DLPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+H+YLNHI  +  QL REPRPFPKL+  R+V++I+DF+ EDF IE Y+PHP I M+MA+
Sbjct:   24 PRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV 313

HSP 2 Score: 128.257 bits (321), Expect = 2.753e-32
Identity = 73/175 (41.71%), Postives = 103/175 (58.86%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGA---DKECE---NGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
             E DL  G  Y  QWR FGA   D E +    GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG FIH+ GD    LN     ++ L  E   FPK++I+  V ++++F  +DF +E
Sbjct:  127 EEGDL--GPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIE 299
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Human
Match: TYMS (thymidylate synthetase [Source:HGNC Symbol;Acc:HGNC:12441])

HSP 1 Score: 320.472 bits (820), Expect = 2.837e-105
Identity = 168/291 (57.73%), Postives = 206/291 (70.79%), Query Frame = 3 
Query:  843 PVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            P   H E+QYL  +++IL  G +  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++GSTN  EL++K V IWDANGS +FL S+G   RE GDLGPVYGFQWRHFGA+Y  M +D                                  LPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+H+YLNHI  +  QL REPRPFPKL+  R+V++I+DF+ EDF IE Y+PHP I M+MA+
Sbjct:   24 PRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESD----------------------------------LPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV 279

HSP 2 Score: 98.5969 bits (244), Expect = 2.691e-22
Identity = 61/167 (36.53%), Postives = 86/167 (51.50%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGADKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
             E DL  G  Y  QWR FGA+                   ++ +  L L+A  P         H L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG FIH+ GD    LN     ++ L  E   FPK++I+  V ++++F  +DF +E
Sbjct:  127 EEGDL--GPVYGFQWRHFGAEY------------------RDMESDLPLMALPP--------CHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIE 265
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Human
Match: TYMS (thymidylate synthetase [Source:HGNC Symbol;Acc:HGNC:12441])

HSP 1 Score: 223.787 bits (569), Expect = 1.331e-68
Identity = 111/172 (64.53%), Postives = 137/172 (79.65%), Query Frame = 3 
Query: 1200 FGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            FG +      DYS QGVDQ+  VI+ IK NP  +  ++ AWNP+DLPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+H+YLNHI  +  QL REPRPFPKL+  R+V++I+DF+ EDF IE Y+PHP I M+MA+
Sbjct:   59 FGMQARYSLRDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV 230

HSP 2 Score: 119.783 bits (299), Expect = 4.087e-30
Identity = 60/144 (41.67%), Postives = 89/144 (61.81%), Query Frame = 3 
Query:  369 NGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHC--LLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
             GVDQ++ +++ +K    DRR+++ AWNP      AL  C  L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG FIH+ GD    LN     ++ L  E   FPK++I+  V ++++F  +DF +E
Sbjct:   73 QGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIE 216
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Celegans
Match: tyms-1 (Thymidylate synthase [Source:UniProtKB/TrEMBL;Acc:Q9N588])

HSP 1 Score: 346.28 bits (887), Expect = 2.713e-115
Identity = 167/287 (58.19%), Postives = 206/287 (71.78%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
             +E +YL+ V  IL  GT+  DRT  GT+S+FG Q  + LRN T+PLLTTKRV+ +G++EELLWF+ GST+G  L  KNV IW+ NG   FL ++G   RE GDLGPVYGFQWRHFGAKY   H DYS QGVDQ+  VI  IK+ P  +  ++ AWNP DL  M LPPCH M QF+V+  ELSC+LYQRSGDMGLGVPFN+ASY +LT+M+A V GLKPG   HTLGD+HVY NH+  +  QL REP  FPK++F R V  I+DF  +   ++DY  HPKI MDMA+
Sbjct:   26 QDEYKYLKQVEQILREGTRRDDRTGTGTISIFGMQSKYCLRNGTIPLLTTKRVYWKGVLEELLWFISGSTDGKLLMEKNVKIWEKNGDRAFLDNLGFTSREEGDLGPVYGFQWRHFGAKYVDCHTDYSGQGVDQLAEVIRQIKEQPDSRRIIMSAWNPSDLGQMVLPPCHTMCQFYVDNGELSCQLYQRSGDMGLGVPFNLASYGLLTHMIAKVCGLKPGTLVHTLGDAHVYSNHVDALKIQLDREPYAFPKIRFTRDVASIDDFTSDMIALDDYKCHPKIPMDMAV 312

HSP 2 Score: 100.908 bits (250), Expect = 3.661e-23
Identity = 60/167 (35.93%), Postives = 92/167 (55.09%), Query Frame = 3 
Query:  297 ESDLIKGENYNSQWRRFGADK-ECEN-----GVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFAL--THCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFV 764
            E DL  G  Y  QWR FGA   +C       GVDQ+  ++  +K++   RR+++ AWNPS   +  L   H + QFYV + +LSC+LYQ SGD+   + F+ A   L T+M+  + GL+PG  +H+ GD     N   + ++ L  E   FPKI+    V+ +++F 
Sbjct:  127 EGDL--GPVYGFQWRHFGAKYVDCHTDYSGQGVDQLAEVIRQIKEQPDSRRIIMSAWNPSDLGQMVLPPCHTMCQFYVDNGELSCQLYQRSGDMGLGVPFNLASYGLLTHMIAKVCGLKPGTLVHTLGDAHVYSNHVDALKIQLDREPYAFPKIRFTRDVASIDDFT 291
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Fly
Match: Ts (gene:FBgn0024920 transcript:FBtr0343784)

HSP 1 Score: 370.933 bits (951), Expect = 1.364e-124
Identity = 187/307 (60.91%), Postives = 231/307 (75.24%), Query Frame = 3 
Query:  801 NFESPGNWHCLCESPVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFV--EGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            N ESP       ++PV   +E+ YL L+R+I+ NG + +DRT+VGT+S+FG QM F +RN + PLLTTKRVF R + EELLWF+ G T+   L AKNVHIWD N S EFL  +G   R  GDLGPVYGFQWRHFGA+Y T   DYS +G+DQ+  VI+ I+ NP  +  ++ AWNP D+P MALPPCHC+ QF+V  +  ELSC+LYQRS DMGLGVPFNIASYA+LT+M+AHVTGLKPG+F HT+GD+HVYLNH+  + +QL R PRPFPKL  KRQV  IEDF+ EDF I DY+PHPKI MDMA+
Sbjct:   21 NGESPSKQ----QAPVN-RDEMHYLDLLRHIIANGEQRMDRTEVGTLSVFGSQMRFDMRN-SFPLLTTKRVFFRAVAEELLWFVAGKTDAKLLQAKNVHIWDGNSSREFLDKMGFTGRAVGDLGPVYGFQWRHFGAQYGTCDDDYSGKGIDQLRQVIDTIRNNPSDRRIIMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTHMIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFEDFQIVDYNPHPKIQMDMAV 321

HSP 2 Score: 115.161 bits (287), Expect = 5.440e-28
Identity = 63/169 (37.28%), Postives = 94/169 (55.62%), Query Frame = 3 
Query:  315 GENYNSQWRRFGA------DKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDK--LSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDL---TNEFPKIKIIGSVSEVENFVLKDFHL 782
            G  Y  QWR FGA      D     G+DQ+  +++ ++    DRR+++ AWNP    + AL   HCL QFYV++ +  LSC+LYQ S D+   + F+ A   L T+M+ H+TGL+PG F+H+ GD    LN     +  L      FPK+ I   V ++E+F  +DF +
Sbjct:  138 GPVYGFQWRHFGAQYGTCDDDYSGKGIDQLRQVIDTIRNNPSDRRIIMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTHMIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFEDFQI 306
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Fly
Match: Ts (gene:FBgn0024920 transcript:FBtr0077679)

HSP 1 Score: 370.933 bits (951), Expect = 1.364e-124
Identity = 187/307 (60.91%), Postives = 231/307 (75.24%), Query Frame = 3 
Query:  801 NFESPGNWHCLCESPVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFV--EGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            N ESP       ++PV   +E+ YL L+R+I+ NG + +DRT+VGT+S+FG QM F +RN + PLLTTKRVF R + EELLWF+ G T+   L AKNVHIWD N S EFL  +G   R  GDLGPVYGFQWRHFGA+Y T   DYS +G+DQ+  VI+ I+ NP  +  ++ AWNP D+P MALPPCHC+ QF+V  +  ELSC+LYQRS DMGLGVPFNIASYA+LT+M+AHVTGLKPG+F HT+GD+HVYLNH+  + +QL R PRPFPKL  KRQV  IEDF+ EDF I DY+PHPKI MDMA+
Sbjct:   21 NGESPSKQ----QAPVN-RDEMHYLDLLRHIIANGEQRMDRTEVGTLSVFGSQMRFDMRN-SFPLLTTKRVFFRAVAEELLWFVAGKTDAKLLQAKNVHIWDGNSSREFLDKMGFTGRAVGDLGPVYGFQWRHFGAQYGTCDDDYSGKGIDQLRQVIDTIRNNPSDRRIIMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTHMIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFEDFQIVDYNPHPKIQMDMAV 321

HSP 2 Score: 115.161 bits (287), Expect = 5.440e-28
Identity = 63/169 (37.28%), Postives = 94/169 (55.62%), Query Frame = 3 
Query:  315 GENYNSQWRRFGA------DKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDK--LSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDL---TNEFPKIKIIGSVSEVENFVLKDFHL 782
            G  Y  QWR FGA      D     G+DQ+  +++ ++    DRR+++ AWNP    + AL   HCL QFYV++ +  LSC+LYQ S D+   + F+ A   L T+M+ H+TGL+PG F+H+ GD    LN     +  L      FPK+ I   V ++E+F  +DF +
Sbjct:  138 GPVYGFQWRHFGAQYGTCDDDYSGKGIDQLRQVIDTIRNNPSDRRIIMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTHMIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFEDFQI 306
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Zebrafish
Match: tyms (thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-040426-59])

HSP 1 Score: 377.481 bits (968), Expect = 4.462e-127
Identity = 185/286 (64.69%), Postives = 221/286 (77.27%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            +E  YL LV  IL NG K  DRT  G +S+FG Q  +SLR D  PLLTTKRVF +GI+EELLWF++GSTN  +L+ K V IWDANGS EFL   G   RE GDLGPVYGFQWRHFGA+Y  MH DYS +GVDQ+  VI+ IK NP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRSGD+GLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+H+Y NHI  + +Q+ REPRPFPKL+ KR+V++I DF  EDF I DY+PHP I M MA+
Sbjct:   34 DERGYLSLVEFILQNGAKKGDRTGTGVISVFGTQARYSLR-DQFPLLTTKRVFWKGILEELLWFIKGSTNAKDLSEKGVRIWDANGSREFLDKNGFTDREEGDLGPVYGFQWRHFGAEYKDMHTDYSGEGVDQLQKVIDTIKSNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVSNGELSCQLYQRSGDIGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHIYTNHIEPLKEQIQREPRPFPKLRIKRKVEQINDFCAEDFEIYDYNPHPTIKMQMAV 318

HSP 2 Score: 117.857 bits (294), Expect = 8.624e-29
Identity = 67/173 (38.73%), Postives = 98/173 (56.65%), Query Frame = 3 
Query:  297 ESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFAL--THCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL 782
            E DL  G  Y  QWR FGA+ +         GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV++ +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG F+H+ GD     N     +  +  E   FPK++I   V ++ +F  +DF +
Sbjct:  133 EGDL--GPVYGFQWRHFGAEYKDMHTDYSGEGVDQLQKVIDTIKSNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVSNGELSCQLYQRSGDIGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHIYTNHIEPLKEQIQREPRPFPKLRIKRKVEQINDFCAEDFEI 303
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Xenopus
Match: cybb (cytochrome b-245, beta polypeptide [Source:Xenbase;Acc:XB-GENE-978453])

HSP 1 Score: 369.392 bits (947), Expect = 5.460e-124
Identity = 182/286 (63.64%), Postives = 224/286 (78.32%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            +E+QYL  +++IL++G +  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++G TN  EL+AK V IWDANGS EFL   G  +RE GDLGPVYGFQWRHFGA+Y   + DYS QGVDQ+ NVI  IK NP  +  ++ +WNPKD+ LMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AHVTGLKPG+F HTLGD+HVYLNH   +  QL R PRPFPKLK  R+V+ I+DF  +DF+IE Y+PHP I M+MA+
Sbjct:   22 DELQYLDQIKHILEHGHRKEDRTGTGTVSVFGMQARYSLR-DQFPLLTTKRVFWKGVLEELLWFIKGCTNSKELSAKGVKIWDANGSREFLDKQGFSNREEGDLGPVYGFQWRHFGAEYKDTYTDYSGQGVDQLRNVIETIKNNPDDRRIIMCSWNPKDISLMALPPCHTLCQFYVIDKELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHVTGLKPGDFVHTLGDAHVYLNHTEPLKLQLQRTPRPFPKLKILRKVENIDDFTADDFLIEGYNPHPSIKMEMAV 306

HSP 2 Score: 123.635 bits (309), Expect = 9.664e-31
Identity = 71/183 (38.80%), Postives = 99/183 (54.10%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHC--LLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            D Q      E DL  G  Y  QWR FGA+ +         GVDQ+  ++  +K    DRR+++ +WNP      AL  C  L QFYV D +LSC+LYQ SGD+   + F+ A   L TYM+ H+TGL+PG F+H+ GD    LN     ++ L      FPK+KI+  V  +++F   DF +E
Sbjct:  112 DKQGFSNREEGDL--GPVYGFQWRHFGAEYKDTYTDYSGQGVDQLRNVIETIKNNPDDRRIIMCSWNPKDISLMALPPCHTLCQFYVIDKELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHVTGLKPGDFVHTLGDAHVYLNHTEPLKLQLQRTPRPFPKLKILRKVENIDDFTADDFLIE 292
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Xenopus
Match: cybb (cytochrome b-245, beta polypeptide [Source:Xenbase;Acc:XB-GENE-978453])

HSP 1 Score: 369.007 bits (946), Expect = 7.110e-124
Identity = 182/286 (63.64%), Postives = 224/286 (78.32%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            +E+QYL  +++IL++G +  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++G TN  EL+AK V IWDANGS EFL   G  +RE GDLGPVYGFQWRHFGA+Y   + DYS QGVDQ+ NVI  IK NP  +  ++ +WNPKD+ LMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AHVTGLKPG+F HTLGD+HVYLNH   +  QL R PRPFPKLK  R+V+ I+DF  +DF+IE Y+PHP I M+MA+
Sbjct:   24 DELQYLDQIKHILEHGHRKEDRTGTGTVSVFGMQARYSLR-DQFPLLTTKRVFWKGVLEELLWFIKGCTNSKELSAKGVKIWDANGSREFLDKQGFSNREEGDLGPVYGFQWRHFGAEYKDTYTDYSGQGVDQLRNVIETIKNNPDDRRIIMCSWNPKDISLMALPPCHTLCQFYVIDKELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHVTGLKPGDFVHTLGDAHVYLNHTEPLKLQLQRTPRPFPKLKILRKVENIDDFTADDFLIEGYNPHPSIKMEMAV 308

HSP 2 Score: 124.02 bits (310), Expect = 8.299e-31
Identity = 71/183 (38.80%), Postives = 99/183 (54.10%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHC--LLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            D Q      E DL  G  Y  QWR FGA+ +         GVDQ+  ++  +K    DRR+++ +WNP      AL  C  L QFYV D +LSC+LYQ SGD+   + F+ A   L TYM+ H+TGL+PG F+H+ GD    LN     ++ L      FPK+KI+  V  +++F   DF +E
Sbjct:  114 DKQGFSNREEGDL--GPVYGFQWRHFGAEYKDTYTDYSGQGVDQLRNVIETIKNNPDDRRIIMCSWNPKDISLMALPPCHTLCQFYVIDKELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHVTGLKPGDFVHTLGDAHVYLNHTEPLKLQLQRTPRPFPKLKILRKVENIDDFTADDFLIE 294
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Xenopus
Match: cybb (cytochrome b-245, beta polypeptide [Source:Xenbase;Acc:XB-GENE-978453])

HSP 1 Score: 253.832 bits (647), Expect = 1.495e-79
Identity = 140/287 (48.78%), Postives = 182/287 (63.41%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMV-AHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            +E+QYL  +++IL++G +  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++G TN  EL+AK V IWDANGS EFL   G  +RE GDLGPVYGFQWRHFGA+Y   + DYS QGVDQ+ NVI  IK NP  +  ++ +WNPK      +  C+C   F                       F +      + ++  H     PG+F HTLGD+HVYLNH   +  QL R PRPFPKLK  R+V+ I+DF  +DF+IE Y+PHP I M+MA+
Sbjct:   24 DELQYLDQIKHILEHGHRKEDRTGTGTVSVFGMQARYSLR-DQFPLLTTKRVFWKGVLEELLWFIKGCTNSKELSAKGVKIWDANGSREFLDKQGFSNREEGDLGPVYGFQWRHFGAEYKDTYTDYSGQGVDQLRNVIETIKNNPDDRRIIMCSWNPK-----VICKCNCCSPFC----------------------FWLLKQCSRSQLINRHGEPTSPGDFVHTLGDAHVYLNHTEPLKLQLQRTPRPFPKLKILRKVENIDDFTADDFLIEGYNPHPSIKMEMAV 282
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Mouse
Match: Tyms (thymidylate synthase [Source:MGI Symbol;Acc:MGI:98878])

HSP 1 Score: 379.407 bits (973), Expect = 4.576e-128
Identity = 187/287 (65.16%), Postives = 229/287 (79.79%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            H E+QYL+ V +IL  G K  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++GSTN  EL++K V IWDANGS +FL S+G   R+ GDLGPVYGFQWRHFGA+Y  M +DYS QGVDQ+  VI+ IK NP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGL+PG+F HTLGD+H+YLNHI  +  QL REPRPFPKLK  R+V+ I+DF++EDF IE Y+PHP I M+MA+
Sbjct:   22 HGELQYLRQVEHILRCGFKKEDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGAEYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIEGYNPHPTIKMEMAV 307

HSP 2 Score: 126.716 bits (317), Expect = 6.039e-32
Identity = 71/175 (40.57%), Postives = 102/175 (58.29%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
             E DL  G  Y  QWR FGA+ +         GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG F+H+ GD    LN     ++ L  E   FPK+KI+  V  +++F ++DF +E
Sbjct:  121 QEGDL--GPVYGFQWRHFGAEYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIE 293
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Mouse
Match: Tyms (thymidylate synthase [Source:MGI Symbol;Acc:MGI:98878])

HSP 1 Score: 184.882 bits (468), Expect = 9.207e-55
Identity = 82/121 (67.77%), Postives = 98/121 (80.99%), Query Frame = 3 
Query: 1200 FGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHI 1562
            FG +      DYS QGVDQ+  VI+ IK NP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGL+PG+F HTLGD+H+YLNHI
Sbjct:   53 FGMQARYSLRDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHI 173

HSP 2 Score: 98.5969 bits (244), Expect = 2.095e-23
Identity = 49/114 (42.98%), Postives = 69/114 (60.53%), Query Frame = 3 
Query:  342 RFGADKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFAL--THCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLN 677
            R+        GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG F+H+ GD    LN
Sbjct:   58 RYSLRDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLN 171
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Mouse
Match: Tyms (thymidylate synthase [Source:MGI Symbol;Acc:MGI:98878])

HSP 1 Score: 135.961 bits (341), Expect = 3.808e-37
Identity = 71/146 (48.63%), Postives = 86/146 (58.90%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNP 1292
            H E+QYL+ V +IL  G K  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++                                   GDLGPVYGFQWRHFGA+Y  M +DYS QGVDQ+  VI+ IK NP
Sbjct:   22 HGELQYLRQVEHILRCGFKKEDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIK----------------------------------EGDLGPVYGFQWRHFGAEYKDMDSDYSGQGVDQLQKVIDTIKTNP 132
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Mouse
Match: Tyms (thymidylate synthase [Source:MGI Symbol;Acc:MGI:98878])

HSP 1 Score: 79.7221 bits (195), Expect = 7.507e-18
Identity = 39/67 (58.21%), Postives = 50/67 (74.63%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLR 1055
            H E+QYL+ V +IL  G K  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++
Sbjct:   22 HGELQYLRQVEHILRCGFKKEDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIK 87
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Mouse
Match: Tyms (thymidylate synthase [Source:MGI Symbol;Acc:MGI:98878])

HSP 1 Score: 79.7221 bits (195), Expect = 2.686e-17
Identity = 39/67 (58.21%), Postives = 50/67 (74.63%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLR 1055
            H E+QYL+ V +IL  G K  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++
Sbjct:   22 HGELQYLRQVEHILRCGFKKEDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIK 87
[Close]
BLAST of Thymidylate synthase-like Protein vs. UniProt/SwissProt
Match: sp|P07607|TYSY_MOUSE (Thymidylate synthase OS=Mus musculus OX=10090 GN=Tyms PE=1 SV=1)

HSP 1 Score: 379.407 bits (973), Expect = 3.201e-127
Identity = 187/287 (65.16%), Postives = 229/287 (79.79%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            H E+QYL+ V +IL  G K  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++GSTN  EL++K V IWDANGS +FL S+G   R+ GDLGPVYGFQWRHFGA+Y  M +DYS QGVDQ+  VI+ IK NP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGL+PG+F HTLGD+H+YLNHI  +  QL REPRPFPKLK  R+V+ I+DF++EDF IE Y+PHP I M+MA+
Sbjct:   22 HGELQYLRQVEHILRCGFKKEDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGAEYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIEGYNPHPTIKMEMAV 307

HSP 2 Score: 126.716 bits (317), Expect = 4.224e-31
Identity = 71/175 (40.57%), Postives = 102/175 (58.29%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
             E DL  G  Y  QWR FGA+ +         GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG F+H+ GD    LN     ++ L  E   FPK+KI+  V  +++F ++DF +E
Sbjct:  121 QEGDL--GPVYGFQWRHFGAEYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIE 293
[Close]
BLAST of Thymidylate synthase-like Protein vs. UniProt/SwissProt
Match: sp|P04818|TYSY_HUMAN (Thymidylate synthase OS=Homo sapiens OX=9606 GN=TYMS PE=1 SV=3)

HSP 1 Score: 378.637 bits (971), Expect = 7.472e-127
Identity = 186/291 (63.92%), Postives = 230/291 (79.04%), Query Frame = 3 
Query:  843 PVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            P   H E+QYL  +++IL  G +  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++GSTN  EL++K V IWDANGS +FL S+G   RE GDLGPVYGFQWRHFGA+Y  M +DYS QGVDQ+  VI+ IK NP  +  ++ AWNP+DLPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+H+YLNHI  +  QL REPRPFPKL+  R+V++I+DF+ EDF IE Y+PHP I M+MA+
Sbjct:   24 PRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV 313

HSP 2 Score: 128.257 bits (321), Expect = 1.322e-31
Identity = 73/175 (41.71%), Postives = 103/175 (58.86%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGA---DKECE---NGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
             E DL  G  Y  QWR FGA   D E +    GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG FIH+ GD    LN     ++ L  E   FPK++I+  V ++++F  +DF +E
Sbjct:  127 EEGDL--GPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIE 299
[Close]
BLAST of Thymidylate synthase-like Protein vs. UniProt/SwissProt
Match: sp|Q89940|TYSY_EHV2 (Thymidylate synthase OS=Equine herpesvirus 2 (strain 86/87) OX=82831 GN=70 PE=3 SV=1)

HSP 1 Score: 377.481 bits (968), Expect = 1.011e-126
Identity = 185/288 (64.24%), Postives = 223/288 (77.43%), Query Frame = 3 
Query:  852 VHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
             H E QYL  VR IL NG +  DRT VGT+S+FG Q  +SLR    PLLTTKRVF RG++EELLWF+RGST+ NEL+A+ V IWDANGS +FL   GLGHRE GDLGPVYGFQWRHFGA Y     DY  QGVDQ+ ++I  IK+NP  +  V+ AWNP DLP MALPPCH + QF+V G ELSC+LYQRSGDMGLGVPFNIASY++LTYMVAH+TGL+PG+F H LGD+HVYLNH+  +  QL+R PRPFP+L+  R+V+ I+DF+ EDF +E YHPH  I M+MA+
Sbjct:    3 THCEHQYLNTVREILANGVRRGDRTGVGTLSVFGDQAKYSLRGQ-FPLLTTKRVFWRGVLEELLWFIRGSTDSNELSARGVKIWDANGSRDFLARAGLGHREPGDLGPVYGFQWRHFGAAYVDSKTDYRGQGVDQLRDLIGEIKRNPESRRLVLTAWNPADLPAMALPPCHLLCQFYVAGGELSCQLYQRSGDMGLGVPFNIASYSLLTYMVAHLTGLEPGDFIHVLGDAHVYLNHVEPLKLQLTRSPRPFPRLRILRRVEDIDDFRAEDFALEGYHPHAAIPMEMAV 289

HSP 2 Score: 123.635 bits (309), Expect = 3.471e-30
Identity = 71/168 (42.26%), Postives = 97/168 (57.74%), Query Frame = 3 
Query:  315 GENYNSQWRRFGA---DKECE---NGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHC--LLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            G  Y  QWR FGA   D + +    GVDQ+  L+  +K+    RRL+L AWNP+     AL  C  L QFYVA  +LSC+LYQ SGD+   + F+ A   L TYM+ H+TGL PG FIH  GD    LN     ++ LT     FP+++I+  V ++++F  +DF LE
Sbjct:  108 GPVYGFQWRHFGAAYVDSKTDYRGQGVDQLRDLIGEIKRNPESRRLVLTAWNPADLPAMALPPCHLLCQFYVAGGELSCQLYQRSGDMGLGVPFNIASYSLLTYMVAHLTGLEPGDFIHVLGDAHVYLNHVEPLKLQLTRSPRPFPRLRILRRVEDIDDFRAEDFALE 275
[Close]
BLAST of Thymidylate synthase-like Protein vs. UniProt/SwissProt
Match: sp|P45352|TYSY_RAT (Thymidylate synthase OS=Rattus norvegicus OX=10116 GN=Tyms PE=1 SV=1)

HSP 1 Score: 376.711 bits (966), Expect = 3.627e-126
Identity = 185/287 (64.46%), Postives = 228/287 (79.44%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            H E+QYL+ V +I+  G K  DRT  GT+S+FG Q  +SLR D  PLLTTKRVF +G++EELLWF++GSTN  EL++K V IWDANGS +FL S+G   R+ GDLGPVYGFQWRHFGA Y  M +DYS QGVDQ+  VI+ IK NP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRSGDMGLGVPFNIASYA+LTYM+AH+TGL+PG+F HTLGD+H+YLNHI  +  QL REPRPFPKL+  R+V+ I+DF++EDF IE Y+PHP I M+MA+
Sbjct:   22 HGELQYLRQVEHIMRCGFKKEDRTGTGTLSVFGMQARYSLR-DEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGADYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVETIDDFKVEDFQIEGYNPHPTIKMEMAV 307

HSP 2 Score: 127.487 bits (319), Expect = 2.173e-31
Identity = 71/175 (40.57%), Postives = 102/175 (58.29%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
             E DL  G  Y  QWR FGAD +         GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV + +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG F+H+ GD    LN     ++ L  E   FPK++I+  V  +++F ++DF +E
Sbjct:  121 QEGDL--GPVYGFQWRHFGADYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVETIDDFKVEDFQIE 293
[Close]
BLAST of Thymidylate synthase-like Protein vs. UniProt/SwissProt
Match: sp|O76511|TYSY_DROME (Thymidylate synthase OS=Drosophila melanogaster OX=7227 GN=Ts PE=1 SV=2)

HSP 1 Score: 370.933 bits (951), Expect = 1.367e-123
Identity = 187/307 (60.91%), Postives = 231/307 (75.24%), Query Frame = 3 
Query:  801 NFESPGNWHCLCESPVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFV--EGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            N ESP       ++PV   +E+ YL L+R+I+ NG + +DRT+VGT+S+FG QM F +RN + PLLTTKRVF R + EELLWF+ G T+   L AKNVHIWD N S EFL  +G   R  GDLGPVYGFQWRHFGA+Y T   DYS +G+DQ+  VI+ I+ NP  +  ++ AWNP D+P MALPPCHC+ QF+V  +  ELSC+LYQRS DMGLGVPFNIASYA+LT+M+AHVTGLKPG+F HT+GD+HVYLNH+  + +QL R PRPFPKL  KRQV  IEDF+ EDF I DY+PHPKI MDMA+
Sbjct:   21 NGESPSKQ----QAPVN-RDEMHYLDLLRHIIANGEQRMDRTEVGTLSVFGSQMRFDMRN-SFPLLTTKRVFFRAVAEELLWFVAGKTDAKLLQAKNVHIWDGNSSREFLDKMGFTGRAVGDLGPVYGFQWRHFGAQYGTCDDDYSGKGIDQLRQVIDTIRNNPSDRRIIMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTHMIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFEDFQIVDYNPHPKIQMDMAV 321

HSP 2 Score: 115.161 bits (287), Expect = 5.453e-27
Identity = 63/169 (37.28%), Postives = 94/169 (55.62%), Query Frame = 3 
Query:  315 GENYNSQWRRFGA------DKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDK--LSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDL---TNEFPKIKIIGSVSEVENFVLKDFHL 782
            G  Y  QWR FGA      D     G+DQ+  +++ ++    DRR+++ AWNP    + AL   HCL QFYV++ +  LSC+LYQ S D+   + F+ A   L T+M+ H+TGL+PG F+H+ GD    LN     +  L      FPK+ I   V ++E+F  +DF +
Sbjct:  138 GPVYGFQWRHFGAQYGTCDDDYSGKGIDQLRQVIDTIRNNPSDRRIIMSAWNPLDIPKMALPPCHCLAQFYVSEKRGELSCQLYQRSADMGLGVPFNIASYALLTHMIAHVTGLKPGDFVHTMGDTHVYLNHVEPLKEQLERTPRPFPKLIIKRQVQDIEDFRFEDFQI 306
[Close]
BLAST of Thymidylate synthase-like Protein vs. TrEMBL
Match: D2A1S6 (Thymidylate synthase-like Protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC007732 PE=3 SV=1)

HSP 1 Score: 400.208 bits (1027), Expect = 1.953e-132
Identity = 190/287 (66.20%), Postives = 225/287 (78.40%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            HEE QYL  ++ I+D G K  DRT VGT S+FG QM +SLR++  PLLTTKRVF RG++EELLWF++GSTN NEL  KNVHIWDAN + EFL S+GL  RE GDLGPVYGFQWRHFGA+Y  MH DY+NQG+DQ+ +VI  I+  P  +  ++ AWNP D+P MALPPCHC++QFFV   ELSC LYQRS DMGLGVPFNIASY++LTYM+AHVTGLKPG F HTLGDSHVYLNH+  + +QL R PRPFP LK KRQV  IEDF  +DF +  Y PHPK+SM MA+
Sbjct:   44 HEEYQYLSHIKLIIDKGVKKSDRTGVGTFSIFGAQMRYSLRDNVFPLLTTKRVFWRGVVEELLWFIKGSTNANELKEKNVHIWDANSTREFLDSVGLNEREEGDLGPVYGFQWRHFGAEYKGMHWDYANQGIDQLAHVIETIRNKPNDRRIIMCAWNPVDIPEMALPPCHCLVQFFVANGELSCLLYQRSADMGLGVPFNIASYSLLTYMIAHVTGLKPGEFIHTLGDSHVYLNHVEPLQEQLKRTPRPFPTLKIKRQVSSIEDFVFDDFELSGYEPHPKLSMPMAV 330

HSP 2 Score: 126.716 bits (317), Expect = 1.892e-28
Identity = 72/173 (41.62%), Postives = 98/173 (56.65%), Query Frame = 3 
Query:  297 ESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL 782
            E DL  G  Y  QWR FGA+ +         G+DQ+  ++  ++ K  DRR+++ AWNP    E AL   HCL+QF+VA+ +LSC LYQ S D+   + F+ A   L TYM+ H+TGL+PG FIH+ GD    LN     +  L      FP +KI   VS +E+FV  DF L
Sbjct:  145 EGDL--GPVYGFQWRHFGAEYKGMHWDYANQGIDQLAHVIETIRNKPNDRRIIMCAWNPVDIPEMALPPCHCLVQFFVANGELSCLLYQRSADMGLGVPFNIASYSLLTYMIAHVTGLKPGEFIHTLGDSHVYLNHVEPLQEQLKRTPRPFPTLKIKRQVSSIEDFVFDDFEL 315
[Close]
BLAST of Thymidylate synthase-like Protein vs. TrEMBL
Match: A0A1S3IGQ1 (thymidylate synthase OS=Lingula unguis OX=7574 GN=LOC106163878 PE=3 SV=1)

HSP 1 Score: 397.127 bits (1019), Expect = 1.976e-131
Identity = 190/287 (66.20%), Postives = 228/287 (79.44%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            HEE  YL  +++I+D+G+K  DRT  GT+S+FG QM +SLR+   PLLTTKRVF RG+ EELLWF++GSTN  EL+ KN+HIWD NGS +FL   GL HRE GDLGPVYGFQWRHFGA+Y  MHADY+ QGVDQ+  VIN IK NP  +  ++ AWNP DLP MALPPCH  +QF+V   ELSC++YQRSGDMGLGVPFNIASY++LTYM+AHVTGLKPG F HTLGD+HVY+NHI  + +QLSREPRPFPKL  KR V  I+DF  EDF +E Y PHPKI+M+MA+
Sbjct:   36 HEEYNYLDAIQHIIDHGSKKEDRTGTGTLSVFGMQMRYSLRDGVFPLLTTKRVFWRGVAEELLWFVKGSTNAKELSDKNIHIWDQNGSRDFLDKSGLKHREEGDLGPVYGFQWRHFGAEYKDMHADYTGQGVDQLQEVINRIKTNPDCRRIIMSAWNPPDLPKMALPPCHSFVQFYVCNGELSCQMYQRSGDMGLGVPFNIASYSLLTYMIAHVTGLKPGEFVHTLGDAHVYVNHIAALKEQLSREPRPFPKLTIKRNVQNIDDFLYEDFELEGYKPHPKIAMEMAV 322

HSP 2 Score: 121.709 bits (304), Expect = 1.016e-26
Identity = 69/183 (37.70%), Postives = 105/183 (57.38%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHC--LLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            D   +K   E DL  G  Y  QWR FGA+ +         GVDQ++ ++N +K     RR+++ AWNP    + AL  C   +QFYV + +LSC++YQ SGD+   + F+ A   L TYM+ H+TGL+PG F+H+ GD    +N   + +  L+ E   FPK+ I  +V  +++F+ +DF LE
Sbjct:  128 DKSGLKHREEGDL--GPVYGFQWRHFGAEYKDMHADYTGQGVDQLQEVINRIKTNPDCRRIIMSAWNPPDLPKMALPPCHSFVQFYVCNGELSCQMYQRSGDMGLGVPFNIASYSLLTYMIAHVTGLKPGEFVHTLGDAHVYVNHIAALKEQLSREPRPFPKLTIKRNVQNIDDFLYEDFELE 308
[Close]
BLAST of Thymidylate synthase-like Protein vs. TrEMBL
Match: E9GKZ0 (Thymidylat_synt domain-containing protein OS=Daphnia pulex OX=6669 GN=DAPPUDRAFT_304293 PE=3 SV=1)

HSP 1 Score: 393.66 bits (1010), Expect = 1.624e-130
Identity = 188/287 (65.51%), Postives = 226/287 (78.75%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            HEE QYL L++N+L NG    DRT  GT S+FG QM FSLR+   PLLTTKRVF RG++EELLWF+RGSTN NEL  K++HIWD N S EFL S+GL  RE GDLGP+YGFQWRHFGA YD MH+DYS QGVDQ+ N+IN I+ NP  +  ++ AWNP  LPLMALPPCHC+ QF+V   ELSC++YQRS DMGLGVPFNIASY++LT M+AHVTGL PG F HTLGD+HVY NHI  +++QL R+PRPFP LK  R+V  I+DF+ +DF++E Y PHPKI MDMA+
Sbjct:    6 HEEHQYLNLIKNLLQNGNVKGDRTGTGTRSIFGAQMRFSLRSGRFPLLTTKRVFWRGLMEELLWFVRGSTNANELREKDIHIWDGNSSREFLDSLGLKDREEGDLGPIYGFQWRHFGANYDNMHSDYSCQGVDQLKNLINTIRTNPDDRRMILTAWNPSALPLMALPPCHCLAQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCMIAHVTGLNPGEFIHTLGDAHVYNNHISALEKQLERQPRPFPTLKIIRKVSDIDDFKADDFVLEGYDPHPKIPMDMAV 292

HSP 2 Score: 127.487 bits (319), Expect = 5.620e-29
Identity = 78/184 (42.39%), Postives = 104/184 (56.52%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGA-------DKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            D   +K   E DL  G  Y  QWR FGA       D  C+ GVDQ++ L+N ++    DRR++L AWNPS     AL   HCL QFYVA+ +LSC++YQ S D+   + F+ A   L T M+ H+TGL PG FIH+ GD     N   + E  L  +   FP +KII  VS++++F   DF LE
Sbjct:   98 DSLGLKDREEGDL--GPIYGFQWRHFGANYDNMHSDYSCQ-GVDQLKNLINTIRTNPDDRRMILTAWNPSALPLMALPPCHCLAQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCMIAHVTGLNPGEFIHTLGDAHVYNNHISALEKQLERQPRPFPTLKIIRKVSDIDDFKADDFVLE 278
[Close]
BLAST of Thymidylate synthase-like Protein vs. TrEMBL
Match: A7RH38 (Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g227987 PE=3 SV=1)

HSP 1 Score: 392.889 bits (1008), Expect = 5.373e-130
Identity = 187/287 (65.16%), Postives = 223/287 (77.70%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            H+E+QYL ++R I++NGT   DRT  GT S+FG QM FSLR+   PLLTTKRVF RG+ EELLWF++G TN   LA KN+HIWDANGS  FL   GL HRE GDLGPVYGFQWRHFGAKY  MH DY+ QGVDQ+  VI+ IK NP  +  +++AWNP D+PLMALPPCH  +QF+V   ELSC+LYQRSGDMGLGVPFNIASY++LTYM+AHV GLKPG+F H+LGD+HVYLNH   +++QL REPR FP L  KRQVD I+ F  +DF I  Y PHPKI M+MA+
Sbjct:   20 HDELQYLDMIRYIMENGTVKTDRTGTGTKSVFGAQMRFSLRDGVFPLLTTKRVFWRGVAEELLWFVKGCTNAKVLADKNIHIWDANGSRGFLDRQGLSHREEGDLGPVYGFQWRHFGAKYVDMHTDYTGQGVDQLAQVIDKIKNNPDDRRIIMLAWNPSDIPLMALPPCHAFVQFYVANGELSCQLYQRSGDMGLGVPFNIASYSLLTYMIAHVCGLKPGDFVHSLGDAHVYLNHFEALEEQLKREPRAFPSLVIKRQVDDIDQFTFDDFEIVGYKPHPKIKMEMAV 306

HSP 2 Score: 120.553 bits (301), Expect = 2.130e-26
Identity = 72/182 (39.56%), Postives = 100/182 (54.95%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGA---DKECE---NGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL 782
            D Q +    E DL  G  Y  QWR FGA   D   +    GVDQ+  +++ +K    DRR++++AWNPS     AL   H  +QFYVA+ +LSC+LYQ SGD+   + F+ A   L TYM+ H+ GL+PG F+HS GD    LN   + E  L  E   FP + I   V +++ F   DF +
Sbjct:  112 DRQGLSHREEGDL--GPVYGFQWRHFGAKYVDMHTDYTGQGVDQLAQVIDKIKNNPDDRRIIMLAWNPSDIPLMALPPCHAFVQFYVANGELSCQLYQRSGDMGLGVPFNIASYSLLTYMIAHVCGLKPGDFVHSLGDAHVYLNHFEALEEQLKREPRAFPSLVIKRQVDDIDQFTFDDFEI 291
[Close]
BLAST of Thymidylate synthase-like Protein vs. TrEMBL
Match: A0A164RGQ7 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daphnia magna OX=35525 GN=APZ42_027308 PE=3 SV=1)

HSP 1 Score: 391.734 bits (1005), Expect = 9.268e-130
Identity = 189/287 (65.85%), Postives = 224/287 (78.05%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            HEE QYL L+RN++ NG    DRT  GT S+FG QM FSLR+   PLLTTKRVF RG++EELLWF+RGSTN  EL  K+V IWD N S EFL S+GL  RE GDLGP+YGFQWRHFGAKYD M++DYS QGVDQ+ N+I  I+ NP  +  ++ AWNP  LPLMALPPCHC+ QF+V   ELSC++YQRS DMGLGVPFNIASY++LT M+AHVTGL PG F HTLGD+HVY NHI  +++QL REPRPFP LK  RQV  I+DF+ EDF++E Y PHPKI MDMA+
Sbjct:    4 HEEHQYLNLIRNLIQNGNVKGDRTGTGTRSIFGAQMRFSLRHGKFPLLTTKRVFWRGLMEELLWFVRGSTNAKELQQKDVRIWDGNSSREFLDSLGLKDREEGDLGPIYGFQWRHFGAKYDNMYSDYSGQGVDQLKNLIETIRTNPDDRRMILTAWNPTALPLMALPPCHCLAQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCMIAHVTGLNPGEFIHTLGDAHVYSNHISALEKQLEREPRPFPILKITRQVPDIDDFKAEDFVLEGYDPHPKIPMDMAV 290

HSP 2 Score: 120.939 bits (302), Expect = 9.153e-27
Identity = 73/183 (39.89%), Postives = 100/183 (54.64%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            D   +K   E DL  G  Y  QWR FGA  +         GVDQ++ L+  ++    DRR++L AWNP+     AL   HCL QFYVA+ +LSC++YQ S D+   + F+ A   L T M+ H+TGL PG FIH+ GD     N   + E  L  E   FP +KI   V ++++F  +DF LE
Sbjct:   96 DSLGLKDREEGDL--GPIYGFQWRHFGAKYDNMYSDYSGQGVDQLKNLIETIRTNPDDRRMILTAWNPTALPLMALPPCHCLAQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCMIAHVTGLNPGEFIHTLGDAHVYSNHISALEKQLEREPRPFPILKITRQVPDIDDFKAEDFVLE 276
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Cavefish
Match: tyms (thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-040426-59])

HSP 1 Score: 370.548 bits (950), Expect = 1.039e-124
Identity = 183/286 (63.99%), Postives = 219/286 (76.57%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            +E  YL  ++ IL NG K  DRT  G +S+FG Q  +SLR D  PLLTTKRVF +GI+EELLWF++GSTN  EL+ K V IWDANGS +FL   G   RE GDLGPVYGFQWRH+GA+Y  MH DYS +GVDQ+  VI+ IK NP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRSGD+GLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+H+YLNHI  +  QL REPRPFPKLK +R V+RI+DF  EDF I  Y P+P I M MA+
Sbjct:   21 DERGYLNQIQYILQNGVKKGDRTGTGVVSVFGLQARYSLR-DQFPLLTTKRVFWKGILEELLWFIKGSTNAKELSEKGVKIWDANGSRDFLDKSGFTEREEGDLGPVYGFQWRHYGAEYKDMHTDYSGKGVDQLQKVIDTIKSNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVSEGELSCQLYQRSGDIGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHIYLNHIEPLKVQLQREPRPFPKLKIRRTVERIDDFTAEDFEICGYDPYPTIKMQMAV 305

HSP 2 Score: 124.02 bits (310), Expect = 4.454e-31
Identity = 69/174 (39.66%), Postives = 101/174 (58.05%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL 782
             E DL  G  Y  QWR +GA+ +         GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV++ +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG F+H+ GD    LN     ++ L  E   FPK+KI  +V  +++F  +DF +
Sbjct:  119 EEGDL--GPVYGFQWRHYGAEYKDMHTDYSGKGVDQLQKVIDTIKSNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVSEGELSCQLYQRSGDIGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHIYLNHIEPLKVQLQREPRPFPKLKIRRTVERIDDFTAEDFEI 290
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Cavefish
Match: tyms (thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-040426-59])

HSP 1 Score: 371.318 bits (952), Expect = 1.257e-124
Identity = 183/286 (63.99%), Postives = 219/286 (76.57%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            +E  YL  ++ IL NG K  DRT  G +S+FG Q  +SLR D  PLLTTKRVF +GI+EELLWF++GSTN  EL+ K V IWDANGS +FL   G   RE GDLGPVYGFQWRH+GA+Y  MH DYS +GVDQ+  VI+ IK NP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRSGD+GLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+H+YLNHI  +  QL REPRPFPKLK +R V+RI+DF  EDF I  Y P+P I M MA+
Sbjct:   46 DERGYLNQIQYILQNGVKKGDRTGTGVVSVFGLQARYSLR-DQFPLLTTKRVFWKGILEELLWFIKGSTNAKELSEKGVKIWDANGSRDFLDKSGFTEREEGDLGPVYGFQWRHYGAEYKDMHTDYSGKGVDQLQKVIDTIKSNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVSEGELSCQLYQRSGDIGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHIYLNHIEPLKVQLQREPRPFPKLKIRRTVERIDDFTAEDFEICGYDPYPTIKMQMAV 330

HSP 2 Score: 124.405 bits (311), Expect = 4.540e-31
Identity = 69/174 (39.66%), Postives = 101/174 (58.05%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL 782
             E DL  G  Y  QWR +GA+ +         GVDQ++ +++ +K    DRR+++ AWNP      AL   H L QFYV++ +LSC+LYQ SGD+   + F+ A   L TYM+ HITGL+PG F+H+ GD    LN     ++ L  E   FPK+KI  +V  +++F  +DF +
Sbjct:  144 EEGDL--GPVYGFQWRHYGAEYKDMHTDYSGKGVDQLQKVIDTIKSNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVSEGELSCQLYQRSGDIGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHIYLNHIEPLKVQLQREPRPFPKLKIRRTVERIDDFTAEDFEI 315
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Sea Lamprey
Match: tyms (thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-040426-59])

HSP 1 Score: 369.392 bits (947), Expect = 6.978e-125
Identity = 179/287 (62.37%), Postives = 218/287 (75.96%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            H+E +YL LVR +L  G    DRT  GT S+FG Q  +SLR    PLLTTKRVF +G++EELLWF++GSTN  EL+AK V IWDANGS  FL   G   RE GDLGPVYGFQWRHFGA Y  MHADY+NQG+DQ+ +VI+ I+ NP  +  ++ AWNPKD+PLMALPPCH + QF+V   ELSC+LYQRS D+GLGVPFNIASYA+LTYM+AH+TGLKPG+F HTLGD+HVY+NH+  +  QL REPRPFP L  +R V  I+DF+ +DF + DY PH  I MDMA+
Sbjct:   28 HDEHEYLDLVRQVLKRGRLKGDRTGTGTRSMFGMQARYSLRGQ-FPLLTTKRVFWKGVLEELLWFIKGSTNAKELSAKGVKIWDANGSRAFLDRQGFPEREEGDLGPVYGFQWRHFGADYTDMHADYANQGLDQLQHVIDTIRTNPDDRRIIMCAWNPKDVPLMALPPCHALCQFYVADGELSCQLYQRSADLGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHVYVNHVEPLKTQLQREPRPFPTLHIRRTVTSIDDFRSDDFELRDYDPHAAIKMDMAV 313

HSP 2 Score: 122.094 bits (305), Expect = 4.866e-31
Identity = 71/183 (38.80%), Postives = 98/183 (53.55%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGADKE------CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            D Q      E DL  G  Y  QWR FGAD           G+DQ++ +++ ++    DRR+++ AWNP      AL   H L QFYVAD +LSC+LYQ S DL   + F+ A   L TYM+ HITGL+PG F+H+ GD    +N     +  L  E   FP + I  +V+ +++F   DF L 
Sbjct:  119 DRQGFPEREEGDL--GPVYGFQWRHFGADYTDMHADYANQGLDQLQHVIDTIRTNPDDRRIIMCAWNPKDVPLMALPPCHALCQFYVADGELSCQLYQRSADLGLGVPFNIASYALLTYMIAHITGLKPGDFVHTLGDAHVYVNHVEPLKTQLQREPRPFPTLHIRRTVTSIDDFRSDDFELR 299
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Yeast
Match: CDC21 (Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; human homolog TYMSOS can complement yeast cdc21 temperature-sensitive mutant at restrictive temperature [Source:SGD;Acc:S000005600])

HSP 1 Score: 337.806 bits (865), Expect = 7.122e-113
Identity = 173/296 (58.45%), Postives = 212/296 (71.62%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGP-QMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFV----EGD-----ELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            EE QYL L + I+D G    DRT  GT+SLF P Q+ FSLR+DT PLLTTK+VF RGII ELLWFL G T+ N L+ + V IWD NGS E+L  +G   R+ GDLGPVYGFQWRHFGAKY T   DY+ QG+DQ+  VI+ +K NP  +  ++ AWNP D   MALPPCH   QF+V    EG+      LSC LYQRS DMGLGVPFNIASYA+LT M+A V  ++PG F HTLGD+HVY +HI  + +Q++R PRPFPKLK KR V  I+DF++ DF IEDY+PHP+I M M++
Sbjct:    9 EEEQYLDLCKRIIDEGEFRPDRTGTGTLSLFAPPQLRFSLRDDTFPLLTTKKVFTRGIILELLWFLAGDTDANLLSEQGVKIWDGNGSREYLDKMGFKDRKVGDLGPVYGFQWRHFGAKYKTCDDDYTGQGIDQLKQVIHKLKTNPYDRRIIMSAWNPADFDKMALPPCHIFSQFYVSFPKEGEGSGKPRLSCLLYQRSCDMGLGVPFNIASYALLTRMIAKVVDMEPGEFIHTLGDAHVYKDHIDALKEQITRNPRPFPKLKIKRDVKDIDDFKLTDFEIEDYNPHPRIQMKMSV 304

HSP 2 Score: 100.908 bits (250), Expect = 5.901e-24
Identity = 61/177 (34.46%), Postives = 96/177 (54.24%), Query Frame = 3 
Query:  315 GENYNSQWRRFGAD-KECEN-----GVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHCLL--QFYVA---------DDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHLE 785
            G  Y  QWR FGA  K C++     G+DQ++ +++ LK   +DRR+++ AWNP+  ++ AL  C +  QFYV+           +LSC LYQ S D+   + F+ A   L T M+  +  + PG FIH+ GD     +   + +  +T     FPK+KI   V ++++F L DF +E
Sbjct:  114 GPVYGFQWRHFGAKYKTCDDDYTGQGIDQLKQVIHKLKTNPYDRRIIMSAWNPADFDKMALPPCHIFSQFYVSFPKEGEGSGKPRLSCLLYQRSCDMGLGVPFNIASYALLTRMIAKVVDMEPGEFIHTLGDAHVYKDHIDALKEQITRNPRPFPKLKIKRDVKDIDDFKLTDFEIE 290
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Nematostella
Match: EDO49153 (Thymidylate synthase [Source:UniProtKB/TrEMBL;Acc:A7RH38])

HSP 1 Score: 392.889 bits (1008), Expect = 7.430e-134
Identity = 187/287 (65.16%), Postives = 223/287 (77.70%), Query Frame = 3 
Query:  855 HEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            H+E+QYL ++R I++NGT   DRT  GT S+FG QM FSLR+   PLLTTKRVF RG+ EELLWF++G TN   LA KN+HIWDANGS  FL   GL HRE GDLGPVYGFQWRHFGAKY  MH DY+ QGVDQ+  VI+ IK NP  +  +++AWNP D+PLMALPPCH  +QF+V   ELSC+LYQRSGDMGLGVPFNIASY++LTYM+AHV GLKPG+F H+LGD+HVYLNH   +++QL REPR FP L  KRQVD I+ F  +DF I  Y PHPKI M+MA+
Sbjct:   20 HDELQYLDMIRYIMENGTVKTDRTGTGTKSVFGAQMRFSLRDGVFPLLTTKRVFWRGVAEELLWFVKGCTNAKVLADKNIHIWDANGSRGFLDRQGLSHREEGDLGPVYGFQWRHFGAKYVDMHTDYTGQGVDQLAQVIDKIKNNPDDRRIIMLAWNPSDIPLMALPPCHAFVQFYVANGELSCQLYQRSGDMGLGVPFNIASYSLLTYMIAHVCGLKPGDFVHSLGDAHVYLNHFEALEEQLKREPRAFPSLVIKRQVDDIDQFTFDDFEIVGYKPHPKIKMEMAV 306

HSP 2 Score: 120.553 bits (301), Expect = 2.946e-30
Identity = 72/182 (39.56%), Postives = 100/182 (54.95%), Query Frame = 3 
Query:  270 DVQKIKCLNESDLIKGENYNSQWRRFGA---DKECE---NGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALT--HCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL 782
            D Q +    E DL  G  Y  QWR FGA   D   +    GVDQ+  +++ +K    DRR++++AWNPS     AL   H  +QFYVA+ +LSC+LYQ SGD+   + F+ A   L TYM+ H+ GL+PG F+HS GD    LN   + E  L  E   FP + I   V +++ F   DF +
Sbjct:  112 DRQGLSHREEGDL--GPVYGFQWRHFGAKYVDMHTDYTGQGVDQLAQVIDKIKNNPDDRRIIMLAWNPSDIPLMALPPCHAFVQFYVANGELSCQLYQRSGDMGLGVPFNIASYSLLTYMIAHVCGLKPGDFVHSLGDAHVYLNHFEALEEQLKREPRAFPSLVIKRQVDDIDQFTFDDFEI 291
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Nematostella
Match: EDO29274 (Thymidylate synthase [Source:UniProtKB/TrEMBL;Acc:A7T438])

HSP 1 Score: 178.718 bits (452), Expect = 1.324e-52
Identity = 94/201 (46.77%), Postives = 121/201 (60.20%), Query Frame = 3 
Query: 1026 IIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQ 1628
            II ELLWFLRG +N   L  +   IW+               RE GDLGPVYG QWR++    D  H       +DQI  V+  +K NP  +  +V AWN  DLP MAL PCH   QF+V   +LSC+LYQRS D+ LGVPFNIASYA+LT+M+A    L+ G+F  T GD H+Y NH   ++ QL+R+P  +P L+ KR+
Sbjct:    1 IILELLWFLRGDSNVRWLQERGCTIWNEWA------------REDGDLGPVYGVQWRNWPTP-DGGH-------IDQIAEVVKQLKSNPDSRRIIVSAWNVADLPQMALMPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLQVGDFVWTGGDCHIYNNHFAQVETQLARQPFAYPTLQIKRR 181

HSP 2 Score: 76.6406 bits (187), Expect = 5.279e-16
Identity = 55/170 (32.35%), Postives = 80/170 (47.06%), Query Frame = 3 
Query:  315 GENYNSQWRRFGADKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFAL--THCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL---EHGEKI 800
            G  Y  QWR +         +DQI  +V  LK     RR+++ AWN +   + AL   H   QFYVAD KLSC+LYQ S D+   + F+ A   L T+ML     L+ G F+ + GD     N     E  L  +   +P ++I      +  +  +DF +   +H E I
Sbjct:   37 GPVYGVQWRNWPTPDG--GHIDQIAEVVKQLKSNPDSRRIIVSAWNVADLPQMALMPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLQVGDFVWTGGDCHIYNNHFAQVETQLARQPFAYPTLQIKRRPDSIFGYEYEDFEVVGYQHHEPI 204
[Close]
BLAST of Thymidylate synthase-like Protein vs. Ensembl Medaka
Match: tyms (thymidylate synthetase [Source:NCBI gene;Acc:101158062])

HSP 1 Score: 357.451 bits (916), Expect = 7.089e-120
Identity = 178/286 (62.24%), Postives = 217/286 (75.87%), Query Frame = 3 
Query:  858 EEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            +E  YL LV++IL NG +  DRT  G +S+FG Q+ +SLR    PLLTTKRVF +GI+EELLWF++GST+  EL+ K V IWDANGS  FL ++G   RE GDLGPVYGFQWRHFGA+Y  MH     +GVDQ+ N+I+ IKKNP  +  ++ AWNPKDLPLMALPPCH + QF+V   ELSC+LYQRS DMGLGVPFNIASYA+LTYM+AH+TGL+PG+F HTLGD+HVY+NH   + +QL R+ RPFPKLK KR V  I+DF  EDF I  Y PHP I M MA+
Sbjct:    9 DERGYLDLVKHILQNGRRKGDRTGTGVLSVFGAQVRYSLRQ--FPLLTTKRVFWKGILEELLWFIKGSTSAKELSDKGVKIWDANGSRAFLDNLGFTDREEGDLGPVYGFQWRHFGAEYKDMH----TKGVDQLQNIIDTIKKNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVCDGELSCQLYQRSADMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHVYVNHAEPLQEQLQRDVRPFPKLKIKRTVANIDDFTAEDFEICGYSPHPAIKMQMAV 288

HSP 2 Score: 124.02 bits (310), Expect = 3.055e-31
Identity = 69/170 (40.59%), Postives = 100/170 (58.82%), Query Frame = 3 
Query:  294 NESDLIKGENYNSQWRRFGADKE--CENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFAL--THCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNE---FPKIKIIGSVSEVENFVLKDFHL 782
             E DL  G  Y  QWR FGA+ +     GVDQ++ +++ +KK   DRR+++ AWNP      AL   H L QFYV D +LSC+LYQ S D+   + F+ A   L TYM+ HITGL+PG F+H+ GD    +N     +  L  +   FPK+KI  +V+ +++F  +DF +
Sbjct:  106 EEGDL--GPVYGFQWRHFGAEYKDMHTKGVDQLQNIIDTIKKNPEDRRIIMCAWNPKDLPLMALPPCHALCQFYVCDGELSCQLYQRSADMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHVYVNHAEPLQEQLQRDVRPFPKLKIKRTVANIDDFTAEDFEI 273
[Close]
BLAST of Thymidylate synthase-like Protein vs. Planmine SMEST
Match: SMESG000063577.1 (SMESG000063577.1)

HSP 1 Score: 1095.11 bits (2831), Expect = 0.000e+0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 3 
Query:   87 MKYPNSIDSKIQLLNQIEQWKKSYCKSGKIIGLQFRLNIDDYKLPIFRLEDFYSIANNLVKDVQKIKCLNESDLIKGENYNSQWRRFGADKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNEFPKIKIIGSVSEVENFVLKDFHLEHGEKINFESPGNWHCLCESPVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVXXXXXXXXXXXXXXXYHPHPKISMDMAL 1715
            MKYPNSIDSKIQLLNQIEQWKKSYCKSGKIIGLQFRLNIDDYKLPIFRLEDFYSIANNLVKDVQKIKCLNESDLIKGENYNSQWRRFGADKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNEFPKIKIIGSVSEVENFVLKDFHLEHGEKINFESPGNWHCLCESPVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVDRIEDFQIEDFIIEDYHPHPKISMDMAL
Sbjct:    1 MKYPNSIDSKIQLLNQIEQWKKSYCKSGKIIGLQFRLNIDDYKLPIFRLEDFYSIANNLVKDVQKIKCLNESDLIKGENYNSQWRRFGADKECENGVDQIEWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHCLLQFYVADDKLSCKLYQSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNWSTEMDLTNEFPKIKIIGSVSEVENFVLKDFHLEHGEKINFESPGNWHCLCESPVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDTLPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTSIGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKKNPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMGLGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLSREPRPFPKLKFKRQVDRIEDFQIEDFIIEDYHPHPKISMDMAL 543
The following BLAST results are available for this feature:
BLAST of Thymidylate synthase-like Protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
TYMS1.556e-12763.92thymidylate synthetase [Source:HGNC Symbol;Acc:HGN... [more]
TYMS2.837e-10557.73thymidylate synthetase [Source:HGNC Symbol;Acc:HGN... [more]
TYMS1.331e-6864.53thymidylate synthetase [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tyms-12.713e-11558.19Thymidylate synthase [Source:UniProtKB/TrEMBL;Acc... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Ts1.364e-12460.91gene:FBgn0024920 transcript:FBtr0343784[more]
Ts1.364e-12460.91gene:FBgn0024920 transcript:FBtr0077679[more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tyms4.462e-12764.69thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cybb5.460e-12463.64cytochrome b-245, beta polypeptide [Source:Xenbase... [more]
cybb7.110e-12463.64cytochrome b-245, beta polypeptide [Source:Xenbase... [more]
cybb1.495e-7948.78cytochrome b-245, beta polypeptide [Source:Xenbase... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tyms4.576e-12865.16thymidylate synthase [Source:MGI Symbol;Acc:MGI:98... [more]
Tyms9.207e-5567.77thymidylate synthase [Source:MGI Symbol;Acc:MGI:98... [more]
Tyms3.808e-3748.63thymidylate synthase [Source:MGI Symbol;Acc:MGI:98... [more]
Tyms7.507e-1858.21thymidylate synthase [Source:MGI Symbol;Acc:MGI:98... [more]
Tyms2.686e-1758.21thymidylate synthase [Source:MGI Symbol;Acc:MGI:98... [more]
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BLAST of Thymidylate synthase-like Protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P07607|TYSY_MOUSE3.201e-12765.16Thymidylate synthase OS=Mus musculus OX=10090 GN=T... [more]
sp|P04818|TYSY_HUMAN7.472e-12763.92Thymidylate synthase OS=Homo sapiens OX=9606 GN=TY... [more]
sp|Q89940|TYSY_EHV21.011e-12664.24Thymidylate synthase OS=Equine herpesvirus 2 (stra... [more]
sp|P45352|TYSY_RAT3.627e-12664.46Thymidylate synthase OS=Rattus norvegicus OX=10116... [more]
sp|O76511|TYSY_DROME1.367e-12360.91Thymidylate synthase OS=Drosophila melanogaster OX... [more]
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BLAST of Thymidylate synthase-like Protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
D2A1S61.953e-13266.20Thymidylate synthase-like Protein OS=Tribolium cas... [more]
A0A1S3IGQ11.976e-13166.20thymidylate synthase OS=Lingula unguis OX=7574 GN=... [more]
E9GKZ01.624e-13065.51Thymidylat_synt domain-containing protein OS=Daphn... [more]
A7RH385.373e-13065.16Predicted protein OS=Nematostella vectensis OX=453... [more]
A0A164RGQ79.268e-13065.85Bifunctional dihydrofolate reductase-thymidylate s... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tyms1.039e-12463.99thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
tyms1.257e-12463.99thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tyms6.978e-12562.37thymidylate synthetase [Source:ZFIN;Acc:ZDB-GENE-0... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
CDC217.122e-11358.45Thymidylate synthase; required for de novo biosynt... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO491537.430e-13465.16Thymidylate synthase [Source:UniProtKB/TrEMBL;Acc... [more]
EDO292741.324e-5246.77Thymidylate synthase [Source:UniProtKB/TrEMBL;Acc... [more]
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BLAST of Thymidylate synthase-like Protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tyms7.089e-12062.24thymidylate synthetase [Source:NCBI gene;Acc:10115... [more]
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BLAST of Thymidylate synthase-like Protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000063577.10.000e+0100.00SMESG000063577.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004470 ID=SMED30004470|Name=Thymidylate synthase-like Protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1810bp
ATTTGAGCGGGAAGTTTAAATGAATAGGAACGGCCTTGTGCATATATAAA
TTCAAACAAAGCCATCTTTCTTAAAACTTTAATAAAATGAAATATCCCAA
TAGCATCGATTCCAAGATACAATTACTTAATCAGATCGAACAATGGAAAA
AATCGTATTGCAAATCTGGAAAAATTATTGGATTGCAATTTCGCCTCAAT
ATTGATGACTATAAACTTCCAATTTTCCGATTAGAAGATTTTTATTCAAT
CGCAAATAATTTAGTGAAAGATGTACAAAAGATCAAATGTTTAAATGAAT
CTGATTTGATCAAAGGCGAAAACTACAATTCACAATGGCGTCGATTTGGT
GCTGATAAAGAATGCGAAAATGGAGTAGATCAGATTGAATGGCTGGTCAA
TATTTTAAAAAAGAAAAATTTTGACCGGAGATTATTGTTAGTGGCTTGGA
ATCCCAGTGGAAATGAGGAGTTTGCGTTGACACATTGCTTGCTGCAGTTC
TACGTGGCTGATGATAAGTTGTCGTGCAAATTGTACCAGTCATCAGGTGA
CCTAGAAAACATAATGCGTTTTGATAGTGCCAGATCAGTTCTGTTCACAT
ATATGCTTTGCCATATTACTGGGTTGCGTCCAGGAATGTTCATACATTCT
TTCGGTGACATAGATTGCGATCTGAACGGTAATTGGAGCACAGAAATGGA
TTTGACCAATGAGTTCCCTAAGATTAAGATAATCGGAAGTGTTAGCGAGG
TTGAAAATTTTGTTTTAAAGGATTTTCATTTGGAACATGGTGAAAAAATA
AATTTCGAATCACCGGGAAATTGGCACTGTTTGTGTGAGTCTCCTGTGGG
TGTCCACGAGGAGGTGCAATATTTGCAGTTAGTTAGGAATATCCTGGACA
ACGGCACTAAATTGATCGATCGAACCAAGGTGGGCACAATGTCTCTATTT
GGCCCCCAAATGTTGTTCAGTTTGCGAAACGATACGCTGCCACTATTGAC
CACTAAGAGAGTATTCGTGCGGGGCATAATAGAGGAGCTGCTCTGGTTTC
TACGGGGATCGACCAATGGTAATGAGCTGGCAGCGAAAAATGTACACATT
TGGGACGCCAACGGCTCAGTTGAGTTCCTCACTAGCATAGGATTAGGACA
TCGGGAAGCGGGGGACCTCGGACCCGTTTACGGTTTCCAGTGGAGACATT
TTGGCGCTAAATACGACACTATGCACGCGGACTACTCGAATCAGGGCGTG
GACCAAATACATAACGTGATCAATGCGATCAAGAAGAATCCAAGGGGTAA
AAATCACGTGGTGATGGCCTGGAACCCCAAAGATCTACCGTTAATGGCGC
TGCCGCCTTGTCATTGCATGATGCAATTCTTTGTCGAGGGGGATGAGCTG
TCGTGCAAGTTGTACCAGAGGTCAGGGGACATGGGATTGGGAGTGCCGTT
CAATATTGCCAGTTACGCCGTTCTCACGTATATGGTCGCCCACGTCACCG
GACTGAAACCTGGGAATTTCTTTCACACTCTGGGGGATTCTCACGTATAT
TTGAATCACATTGGCGTTATTGATCAGCAGCTCTCAAGAGAACCGCGTCC
GTTTCCCAAGCTGAAATTCAAGCGTCAAGTTGATCGAATTGAAGATTTCC
AAATAGAAGACTTCATTATCGAGGACTATCACCCCCATCCTAAAATTTCA
ATGGATATGGCTCTATAATTCTCGGCACCGAGAATCTAGCTAGTCTGATT
TATGTTTTGATTGAGAACTTTTTATAAATCTTTACTGAATAAATTATTTT
GGTAAAAAAA
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protein sequence of SMED30004470-orf-1

>SMED30004470-orf-1 ID=SMED30004470-orf-1|Name=SMED30004470-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=544bp
MKYPNSIDSKIQLLNQIEQWKKSYCKSGKIIGLQFRLNIDDYKLPIFRLE
DFYSIANNLVKDVQKIKCLNESDLIKGENYNSQWRRFGADKECENGVDQI
EWLVNILKKKNFDRRLLLVAWNPSGNEEFALTHCLLQFYVADDKLSCKLY
QSSGDLENIMRFDSARSVLFTYMLCHITGLRPGMFIHSFGDIDCDLNGNW
STEMDLTNEFPKIKIIGSVSEVENFVLKDFHLEHGEKINFESPGNWHCLC
ESPVGVHEEVQYLQLVRNILDNGTKLIDRTKVGTMSLFGPQMLFSLRNDT
LPLLTTKRVFVRGIIEELLWFLRGSTNGNELAAKNVHIWDANGSVEFLTS
IGLGHREAGDLGPVYGFQWRHFGAKYDTMHADYSNQGVDQIHNVINAIKK
NPRGKNHVVMAWNPKDLPLMALPPCHCMMQFFVEGDELSCKLYQRSGDMG
LGVPFNIASYAVLTYMVAHVTGLKPGNFFHTLGDSHVYLNHIGVIDQQLS
REPRPFPKLKFKRQVDRIEDFQIEDFIIEDYHPHPKISMDMAL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000101muscle cell
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002142parenchyma
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR012262DHFR-TS
IPR036926Thymidate_synth/dCMP_Mease_sf
IPR000398Thymidylate_synthase
IPR023451Thymidate_synth/dCMP_Mease
Vocabulary: molecular function
TermDefinition
GO:0004146dihydrofolate reductase activity
GO:0004799thymidylate synthase activity
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006730one-carbon metabolic process
GO:0006231dTMP biosynthetic process
GO:0032259methylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000398Thymidylate synthasePRINTSPR00108THYMDSNTHASEcoord: 476..493
score: 55.56
coord: 420..435
score: 61.17
coord: 438..464
score: 79.43
coord: 301..322
score: 70.6
coord: 394..413
score: 40.21
IPR000398Thymidylate synthaseTIGRFAMTIGR03284TIGR03284coord: 261..542
e-value: 9.0E-98
score: 325.0
coord: 62..234
e-value: 3.4E-32
score: 109.8
IPR012262Bifunctional dihydrofolate reductase/thymidylate synthasePIRSFPIRSF000389DHFR-TScoord: 60..243
e-value: 1.1E-33
score: 114.2
coord: 236..543
e-value: 1.5E-149
score: 496.5
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilyGENE3DG3DSA:3.30.572.10coord: 250..543
e-value: 1.6E-131
score: 439.9
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilyGENE3DG3DSA:3.30.572.10coord: 47..237
e-value: 2.0E-32
score: 114.2
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilySUPERFAMILYSSF55831Thymidylate synthase/dCMP hydroxymethylasecoord: 70..235
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilySUPERFAMILYSSF55831Thymidylate synthase/dCMP hydroxymethylasecoord: 257..542
IPR023451Thymidylate synthase/dCMP hydroxymethylase domainPFAMPF00303Thymidylat_syntcoord: 77..234
e-value: 1.5E-25
score: 89.9
coord: 261..542
e-value: 6.2E-107
score: 356.7
IPR023451Thymidylate synthase/dCMP hydroxymethylase domainCDDcd00351TS_Pyrimidine_HMasecoord: 261..492
e-value: 2.4282E-103
score: 307.666
NoneNo IPR availablePANTHERPTHR11548:SF2THYMIDYLATE SYNTHASEcoord: 256..542
NoneNo IPR availablePANTHERPTHR11548:SF2THYMIDYLATE SYNTHASEcoord: 66..234
NoneNo IPR availablePANTHERPTHR11548FAMILY NOT NAMEDcoord: 256..542
NoneNo IPR availablePANTHERPTHR11548FAMILY NOT NAMEDcoord: 66..234