SMED30004453
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004453 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13Note: Hover over icons to view figure legend
Alignments
SMED30004453 aligns in the following genomic locations:
Homology
BLAST of SMED30004453 vs. TrEMBL
Match: I1ZIC2 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 232.261 bits (591), Expect = 1.910e-71 Identity = 131/294 (44.56%), Postives = 180/294 (61.22%), Query Frame = 1 Query: 64 MKHIIISAKKLRQAMSDHYCTDHVVELQLIAAVLLEKDIGDPYKEYDLSAAPKFTKYFNRSWNLTTMAKKKNEQKKTNVLKYIQWKYDPVSYSVDANVLKTIYCIYSFYSKNRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPQNRNEGKGANVRAWLDWVYSDSQKTLPNHIFHSIAFIFRTYKRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNILAD 945 M H I+ A++LR+AM + Y DHVVELQLI AVLLE I E + A +FT YFN+ WNL T +K N+ KK V+ YI+WKY+ + V +TI IYSFYSKNR V + I + +++F +VE +L LL+C C HPS+ F D + E+Y L T+ NLDHIVE QII+ VL + ++++I +AAKFA YFN DWNF+I+P+ N KG+ V+ WL +T P I I +TY+ NR V +I+NDNS+ ++R LF VE L N+L + Sbjct: 1 MGHTIL-ARELREAMKEQYSIDHVVELQLIVAVLLENGID----EKQIEIATEFTNYFNKEWNLQTKPRKDNQSKKVAVINYIKWKYNSLIGEPSTFVFETISEIYSFYSKNRTLVSNEIKGVKEPLKQKFLQVEKALINLLECRNCKHPSLKF-DFEIYEKEIYKHLSTKNNLDHIVEFQIIADVLAKNDIKNIDVAAKFAKYFNKDWNFKIIPREENYNKGSIVKNWL----KSDLRTKPG----VINAISQTYQSNRETVKFFIKNDNSLNQSERDLFIKVEITLNNLLTN 280
BLAST of SMED30004453 vs. Planmine SMEST
Match: SMESG000038305.1 (SMESG000038305.1) HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0 Identity = 271/292 (92.81%), Postives = 280/292 (95.89%), Query Frame = 1 Query: 64 MKHIIISAKKLRQAMSDHYCTDHVVELQLIAAVLLEKDIGDPYKEYDLSAAPKFTKYFNRSWNLTTMAKKKNEQKKTNVLKYIQWKYDPVSYSVDANVLKTIYCIYSFYSKNRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPQNRNEGKGANVRAWLDWVYSDSQKTLPNHIFHSIAFIFRTYKRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL 939 MK IIISAKKLRQAMS+HYCTDHVVELQLIAAVLLEKDIGDPYK+YDLSAAPKFTKYFNRSWNLTTM KK+NEQKKTNVLKYI+WKYDPVS SVD NVLKTIY IYSFY +NRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVP+N N+ KGANV AWLDWVYS+SQKTLP +IFHSI FIFRTY RNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL Sbjct: 1 MKDIIISAKKLRQAMSNHYCTDHVVELQLIAAVLLEKDIGDPYKKYDLSAAPKFTKYFNRSWNLTTMTKKENEQKKTNVLKYIRWKYDPVSCSVDDNVLKTIYYIYSFYFENRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPENINKRKGANVHAWLDWVYSNSQKTLPIYIFHSIGFIFRTYNRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL 292
BLAST of SMED30004453 vs. Planmine SMEST
Match: SMESG000038305.1 (SMESG000038305.1) HSP 1 Score: 573.163 bits (1476), Expect = 0.000e+0 Identity = 271/292 (92.81%), Postives = 280/292 (95.89%), Query Frame = 1 Query: 64 MKHIIISAKKLRQAMSDHYCTDHVVELQLIAAVLLEKDIGDPYKEYDLSAAPKFTKYFNRSWNLTTMAKKKNEQKKTNVLKYIQWKYDPVSYSVDANVLKTIYCIYSFYSKNRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPQNRNEGKGANVRAWLDWVYSDSQKTLPNHIFHSIAFIFRTYKRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL 939 MK IIISAKKLRQAMS+HYCTDHVVELQLIAAVLLEKDIGDPYK+YDLSAAPKFTKYFNRSWNLTTM KK+NEQKKTNVLKYI+WKYDPVS SVD NVLKTIY IYSFY +NRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVP+N N+ KGANV AWLDWVYS+SQKTLP +IFHSI FIFRTY RNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL Sbjct: 1 MKDIIISAKKLRQAMSNHYCTDHVVELQLIAAVLLEKDIGDPYKKYDLSAAPKFTKYFNRSWNLTTMTKKENEQKKTNVLKYIRWKYDPVSCSVDDNVLKTIYYIYSFYFENRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPENINKRKGANVHAWLDWVYSNSQKTLPIYIFHSIGFIFRTYNRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL 292
BLAST of SMED30004453 vs. Planmine SMEST
Match: SMESG000038305.1 (SMESG000038305.1) HSP 1 Score: 567 bits (1460), Expect = 0.000e+0 Identity = 271/292 (92.81%), Postives = 280/292 (95.89%), Query Frame = 1 Query: 64 MKHIIISAKKLRQAMSDHYCTDHVVELQLIAAVLLEKDIGDPYKEYDLSAAPKFTKYFNRSWNLTTMAKKKNEQKKTNVLKYIQWKYDPVSYSVDANVLKTIYCIYSFYSKNRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPQNRNEGKGANVRAWLDWVYSDSQKTLPNHIFHSIAFIFRTYKRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL 939 MK IIISAKKLRQAMS+HYCTDHVVELQLIAAVLLEKDIGDPYK+YDLSAAPKFTKYFNRSWNLTTM KK+NEQKKTNVLKYI+WKYDPVS SVD NVLKTIY IYSFY +NRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVP+N N+ KGANV AWLDWVYS+SQKTLP +IFHSI FIFRTY RNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL Sbjct: 1 MKDIIISAKKLRQAMSNHYCTDHVVELQLIAAVLLEKDIGDPYKKYDLSAAPKFTKYFNRSWNLTTMTKKENEQKKTNVLKYIRWKYDPVSCSVDDNVLKTIYYIYSFYFENRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPENINKRKGANVHAWLDWVYSNSQKTLPIYIFHSIGFIFRTYNRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNIL 292
BLAST of SMED30004453 vs. Planmine SMEST
Match: SMESG000035867.1 (SMESG000035867.1) HSP 1 Score: 236.498 bits (602), Expect = 1.071e-76 Identity = 132/294 (44.90%), Postives = 181/294 (61.56%), Query Frame = 1 Query: 64 MKHIIISAKKLRQAMSDHYCTDHVVELQLIAAVLLEKDIGDPYKEYDLSAAPKFTKYFNRSWNLTTMAKKKNEQKKTNVLKYIQWKYDPVSYSVDANVLKTIYCIYSFYSKNRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPQNRNEGKGANVRAWLDWVYSDSQKTLPNHIFHSIAFIFRTYKRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNILAD 945 M H I+ A++LR+AM + Y DHVVELQLI AVLLE I E + A +FT YFN+ WNL T +K N+ KK V+ YI+WKY+ + V +TI IYSFYSKNR V + I + +++F +VE +L LL+C C HPS+ F D + E+Y L T+ NLDHIVE QII+ VL + ++++I +AAKFA YFN DWNF+I+P+ N KG+ V+ WL +T P I I +TY+ NR V +I+NDNS+ ++R LFF VE L N+L + Sbjct: 1 MGHTIL-ARELREAMKEQYSIDHVVELQLIVAVLLESGID----EKQIEIATEFTNYFNKEWNLQTKPRKDNQSKKVAVINYIKWKYNSLIGEPSTFVFETISEIYSFYSKNRTLVSNEIKGVKEPLKQKFLQVEKALINLLECRNCKHPSLKF-DFEIYEKEIYKHLSTKKNLDHIVEFQIIADVLAKNDIKNIDVAAKFAKYFNKDWNFKIIPREENSNKGSIVKNWL----KSDLRTKPG----VINAISQTYQSNRETVKFFIKNDNSLNQSERDLFFKVEITLNNLLTN 280
BLAST of SMED30004453 vs. Planmine SMEST
Match: SMESG000038300.1 (SMESG000038300.1) HSP 1 Score: 235.728 bits (600), Expect = 2.008e-76 Identity = 132/294 (44.90%), Postives = 181/294 (61.56%), Query Frame = 1 Query: 64 MKHIIISAKKLRQAMSDHYCTDHVVELQLIAAVLLEKDIGDPYKEYDLSAAPKFTKYFNRSWNLTTMAKKKNEQKKTNVLKYIQWKYDPVSYSVDANVLKTIYCIYSFYSKNRYQVLDAIDNDEIGHRKRFREVEYSLFMLLKCSRCPHPSINFGDIRLETSELYHCLQTQYNLDHIVELQIISTVLTEKNVRDIHIAAKFAGYFNDDWNFQIVPQNRNEGKGANVRAWLDWVYSDSQKTLPNHIFHSIAFIFRTYKRNRSIVINYIRNDNSITGNDRRLFFNVEKDLYNILAD 945 M H I+ A++LR+AM + Y DHVVELQLI AVLLE I E + A +FT YFN+ WNL T +K N+ KK V+ YI+WKY+ + V +TI IYSFYSKNR V + I + +++F +VE +L LL+C C HPS+ F D + E+Y L T+ NLDHIVE QII+ VL + ++++I +AAKFA YFN DWNF+I+P+ N KG+ V+ WL +T P I I +TY+ NR V +I+NDNS+ ++R LFF VE L N+L + Sbjct: 1 MGHTIL-ARELREAMKEQYSIDHVVELQLIVAVLLENGID----EKQIEIATEFTNYFNKEWNLQTKPRKDNQSKKVAVINYIKWKYNSLIGEPSTFVFETISEIYSFYSKNRTLVSNEIKGVKEPLKQKFLQVEKALINLLECRNCKHPSLKF-DFEIYEKEIYKHLSTKKNLDHIVEFQIIADVLAKNDIKNIDVAAKFAKYFNKDWNFKIIPREENYNKGSIVKNWL----KSDLRTKPG----VINVISQTYQSNRETVKFFIKNDNSLNQSERDLFFKVEITLNNLLTN 280 The following BLAST results are available for this feature:
BLAST of SMED30004453 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004453 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004453 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of SMED30004453 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004453 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004453 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004453 ID=SMED30004453|Name=SMED30004453|organism=Schmidtea mediterranea sexual|type=transcript|length=960bpback to top protein sequence of SMED30004453-orf-1 >SMED30004453-orf-1 ID=SMED30004453-orf-1|Name=SMED30004453-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=305bp MAFQLERFTKMKHIIISAKKLRQAMSDHYCTDHVVELQLIAAVLLEKDIGback to top Annotated Terms
The following terms have been associated with this transcript:
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