EF-hand domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004423 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Match: CG13449 (gene:FBgn0036520 transcript:FBtr0332094) HSP 1 Score: 47.7506 bits (112), Expect = 1.771e-6 Identity = 44/183 (24.04%), Postives = 80/183 (43.72%), Query Frame = 1 Query: 139 DFEIEPYTSLSFSQNLQRNG-RFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKK----------------------HKTNENFIPFRRFELAINDVSICAVGHEDISMLLEV----SQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQKEGVEKADFHSLKFKLKNVNI 606 D ++ P T+ + ++ RF +P+D+ L+ MTP +YL +Y V +R+ LY+ VF K +K E + ++ A+ DV E++ L + +Q DF + G+ + A+RL + D D + +EKADF+S++ + N + Sbjct: 13 DDKVSPETTSKMPRKFSKSATRFDVPMDLSMLKNMTPWDYLSKYVWVSQQRKQLYKRVFLKYLSRIEEPESELAAMGNDELPEYKYRERTLVWQSLPQALEDVLEFHGTEENVQGTLRMLGYDAQASGDLDF--RAWCGIVSFAERLALADESGSD----DSCDELEKADFNSMEQIMPNFTV 189
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Match: CG13449 (gene:FBgn0036520 transcript:FBtr0075590) HSP 1 Score: 46.595 bits (109), Expect = 9.733e-6 Identity = 44/183 (24.04%), Postives = 80/183 (43.72%), Query Frame = 1 Query: 139 DFEIEPYTSLSFSQNLQRNG-RFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKK----------------------HKTNENFIPFRRFELAINDVSICAVGHEDISMLLEV----SQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQKEGVEKADFHSLKFKLKNVNI 606 D ++ P T+ + ++ RF +P+D+ L+ MTP +YL +Y V +R+ LY+ VF K +K E + ++ A+ DV E++ L + +Q DF + G+ + A+RL + D D + +EKADF+S++ + N + Sbjct: 375 DDKVSPETTSKMPRKFSKSATRFDVPMDLSMLKNMTPWDYLSKYVWVSQQRKQLYKRVFLKYLSRIEEPESELAAMGNDELPEYKYRERTLVWQSLPQALEDVLEFHGTEENVQGTLRMLGYDAQASGDLDF--RAWCGIVSFAERLALADESGSD----DSCDELEKADFNSMEQIMPNFTV 551
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: C3ZHU2 (EF-hand domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_89390 PE=4 SV=1) HSP 1 Score: 100.523 bits (249), Expect = 1.530e-20 Identity = 60/165 (36.36%), Postives = 95/165 (57.58%), Query Frame = 1 Query: 133 MQDFEIEPYTSLSFSQN---LQRN-GRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKK-HKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPE--DEKILDQKEGVEKADFHSLKFKLKNVNI 606 +Q+FE++ ++ Q L R RF+LP+D+R LE M+P EYL Y +V RR+ LY+ F K + + I + + A+ + + V E + +L+V Q + T F LF VCALA+R++++ FV E D+ + QKE VE ADF +L++KL V + Sbjct: 587 VQEFEVKRPKQQAWLQGRLALSRQISRFELPMDMRLLEGMSPIEYLSNYCIVSKRRQNLYKQSFTKADRDRDGIISVKELDKALKQIHVDGVTTEQVQKILDVIQATDDTKFDAKLFTAVCALAERMLYTYFVTEDTDDIMYSQKERVEDADFCALEWKLHGVKV 751
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1S3KHV3 (uncharacterized protein LOC106181848 OS=Lingula unguis OX=7574 GN=LOC106181848 PE=4 SV=1) HSP 1 Score: 93.5893 bits (231), Expect = 1.009e-19 Identity = 46/140 (32.86%), Postives = 83/140 (59.29%), Query Frame = 1 Query: 187 QRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPED--EKILDQKEGVEKADFHSLKFKLKNV 600 ++ RF+LP+D++ LE M+P +YL +Y V +R A+Y+ F KH+ + F++ + +NDV + E + + Q+ E T LF G+ AL +RL++++F+ ED + + QKE +E ADF +L++K + + Sbjct: 47 KQASRFELPMDMKLLETMSPQDYLREYCKVSSKRTAIYKKFFDKHREKTFNVTFQKLDTVLNDVLAHPISKETFQDVCSLVQLDENTSIDYKLFAGIAALCERLLYTRFITEDTADMVEYQKERIECADFTALEWKFQGI 186
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A3S1B4L8 (Uncharacterized protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_012476 PE=4 SV=1) HSP 1 Score: 95.5153 bits (236), Expect = 3.718e-19 Identity = 51/142 (35.92%), Postives = 86/142 (60.56%), Query Frame = 1 Query: 190 RNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTNENFIPFRR-FELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQ--KEGVEKADFHSLKFKLKNVNI 606 ++ RF+LP+D++ LEKM+P +YL +Y V+ RR+ LYQ +F+KH+ I + LA+ +V + A+ ++ L ++ I E T LF G+ ALA+R+++ ++ ED + KE VE ADF +L++KL V + Sbjct: 246 QSSRFELPMDMKSLEKMSPCDYLRKYCVISNRRKTLYQKIFQKHRERGGHILGKNPLSLALQEVLVNALSERHLAELCDILDIDETTSVDLQLFSGMAALAERILYPDYITEDTSECTEYHKEKVECADFCALQWKLHGVQV 387
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1S3K3G3 (serine/arginine repetitive matrix protein 1 OS=Lingula unguis OX=7574 GN=LOC106178440 PE=4 SV=1) HSP 1 Score: 96.6709 bits (239), Expect = 3.723e-19 Identity = 47/140 (33.57%), Postives = 83/140 (59.29%), Query Frame = 1 Query: 187 QRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPED--EKILDQKEGVEKADFHSLKFKLKNV 600 ++ RF+LP+D++ LE M P +YL +Y V +R A+Y+ F KH+ + F++ + +NDV +G E + + Q+ E T LF G+ AL +RL++++F+ ED + + QKE +E ADF +L++K + + Sbjct: 565 KQASRFELPMDMKLLETMNPQDYLREYCKVSSKRTAIYKKFFDKHREKTFNVTFQKLDTVLNDVLAHPIGKETFQDVCSLVQLDENTSIDYKLFAGIAALCERLLYTRFITEDTADMVEYQKERIECADFTALEWKFQGI 704
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A7STV8 (Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g247384 PE=4 SV=1) HSP 1 Score: 92.0485 bits (227), Expect = 1.234e-17 Identity = 45/142 (31.69%), Postives = 86/142 (60.56%), Query Frame = 1 Query: 187 QRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKK-HKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQ-KEGVEKADFHSLKFKLKNVNI 606 ++ RF+LP+D++ LE M+ +YL QY ++ RR+ALY+ +F+K K + I +R + A+ +V + ++ + + E+ + + T F+ F + A ++R++F+ FV E+ + L+ +E +E+ADF SLK KL + Sbjct: 754 KQTSRFELPMDVKVLESMSAMDYLTQYCIISTRRKALYKNIFQKTDKDRDGIISYRELDKALKEVHVDSINTSQVRQIAEMVEADDKTKFNLKQFSAIAAFSERILFADFVTEETRGLNNTREIIEEADFCSLKSKLDGFKV 895
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Match: EDO32837 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STV8]) HSP 1 Score: 92.0485 bits (227), Expect = 1.707e-21 Identity = 45/142 (31.69%), Postives = 86/142 (60.56%), Query Frame = 1 Query: 187 QRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKK-HKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQ-KEGVEKADFHSLKFKLKNVNI 606 ++ RF+LP+D++ LE M+ +YL QY ++ RR+ALY+ +F+K K + I +R + A+ +V + ++ + + E+ + + T F+ F + A ++R++F+ FV E+ + L+ +E +E+ADF SLK KL + Sbjct: 754 KQTSRFELPMDVKVLESMSAMDYLTQYCIISTRRKALYKNIFQKTDKDRDGIISYRELDKALKEVHVDSINTSQVRQIAEMVEADDKTKFNLKQFSAIAAFSERILFADFVTEETRGLNNTREIIEEADFCSLKSKLDGFKV 895
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000004207.1 (SMESG000004207.1) HSP 1 Score: 325.094 bits (832), Expect = 1.543e-114 Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 1 Query: 133 MQDFEIEPYTSLSFSQNLQRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQKEGVEKADFHSLKFKLKNVNI 606 MQDFEIEPYTSLSFSQNLQRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQKEGVEKADFHSLKFKLKNVNI Sbjct: 1 MQDFEIEPYTSLSFSQNLQRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTNENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDEKILDQKEGVEKADFHSLKFKLKNVNI 158
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000043291.1 (SMESG000043291.1) HSP 1 Score: 53.1434 bits (126), Expect = 4.022e-8 Identity = 37/139 (26.62%), Postives = 71/139 (51.08%), Query Frame = 1 Query: 199 RFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTN-ENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDE-KILDQKEGV-EKADFHSLKFKLKNVNI 606 +F LP ++ L+ YL +YS + R A YQ + K++ + E +P + L IND+ + + + L ++ ++KE + VCA+A+R+ F+ + +D + +G EK DF ++ KL+N ++ Sbjct: 177 KFNLPTNLELLKDDDTLSYLEKYSWICDSRLAYYQKEYSKYRDHRETSLPLSKLFLLINDILANTLTEKQFTTLCQILELKENSTIQFKHIFHVCAVAERMFFTMNLSQDSGNAFEINKGFQEKIDFTNIIHKLQNYSV 315
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000043291.1 (SMESG000043291.1) HSP 1 Score: 52.7582 bits (125), Expect = 5.180e-8 Identity = 37/139 (26.62%), Postives = 71/139 (51.08%), Query Frame = 1 Query: 199 RFQLPLDIRKLEKMTPSEYLCQYSVVLLRREALYQTVFKKHKTN-ENFIPFRRFELAINDVSICAVGHEDISMLLEVSQIKEGTDFSESLFRGVCALADRLIFSKFVPEDE-KILDQKEGV-EKADFHSLKFKLKNVNI 606 +F LP ++ L+ YL +YS + R A YQ + K++ + E +P + L IND+ + + + L ++ ++KE + VCA+A+R+ F+ + +D + +G EK DF ++ KL+N ++ Sbjct: 193 KFNLPTNLELLKDDDTLSYLEKYSWICDSRLAYYQKEYSKYRDHRETSLPLSKLFLLINDILANTLTEKQFTTLCQILELKENSTIQFKHIFHVCAVAERMFFTMNLSQDSGNAFEINKGFQEKIDFTNIIHKLQNYSV 331 The following BLAST results are available for this feature:
BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004423 ID=SMED30004423|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=705bpback to top protein sequence of SMED30004423-orf-1 >SMED30004423-orf-1 ID=SMED30004423-orf-1|Name=SMED30004423-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=170bp MQDFEIEPYTSLSFSQNLQRNGRFQLPLDIRKLEKMTPSEYLCQYSVVLLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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