SMED30004411
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004411 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of SMED30004411 vs. TrEMBL
Match: A0A504YQH4 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_04651 PE=4 SV=1) HSP 1 Score: 78.1814 bits (191), Expect = 9.423e-12 Identity = 36/70 (51.43%), Postives = 47/70 (67.14%), Query Frame = 1 Query: 1063 LIGIKSCPDWCSTVETECPYFTPID--GNGGEPAFLCDESHYHHNARYRL--NTNASCGADMPWNHCCYS 1260 L+ ++ C WC+ VET CPYF P D NGGEPAFLCDE HYHH++RY++ ++ C A+ CCYS Sbjct: 126 LVDVEPCVSWCTRVETLCPYFNPADPTANGGEPAFLCDEGHYHHSSRYQVIYRSDQLCEAE-----CCYS 190
BLAST of SMED30004411 vs. TrEMBL
Match: A0A4E0S2X1 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_002828 PE=4 SV=1) HSP 1 Score: 78.1814 bits (191), Expect = 3.062e-11 Identity = 36/70 (51.43%), Postives = 47/70 (67.14%), Query Frame = 1 Query: 1063 LIGIKSCPDWCSTVETECPYFTPID--GNGGEPAFLCDESHYHHNARYRL--NTNASCGADMPWNHCCYS 1260 L+ ++ C WC+ VET CPYF P D NGGEPAFLCDE HYHH++RY++ ++ C A+ CCYS Sbjct: 463 LVDVEPCVSWCTRVETLCPYFNPADPTANGGEPAFLCDEGHYHHSSRYQVIYRSDQLCEAE-----CCYS 527
BLAST of SMED30004411 vs. TrEMBL
Match: A0A095CEC9 (Uncharacterized protein OS=Schistosoma haematobium OX=6185 GN=MS3_09629 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 2.876e-10 Identity = 45/122 (36.89%), Postives = 66/122 (54.10%), Query Frame = 1 Query: 1072 IKSCPDWCSTVETECPYFTPID--GNGGEPAFLCDESHYHHNARYR-----LNTNASCGADMPWNHCCY--------STELLDETQSTSNYRFFSQFSPNSE--TNQKSCISEQKNCLYKLI 1386 ++ C WC+ VET CPY P D NGGEPAFLCDESHYHH++RY+ +NT C ++ CC+ S + D+ Q ++ F +S + E + SC + CL++L+ Sbjct: 412 VQPCISWCTQVETVCPYLNPADPTSNGGEPAFLCDESHYHHSSRYQAVYWDVNT---CESE-----CCFGVSDSILLSVSIPDKKQYSTPTDFLLSYSSDVEESNDNDSCSHLRDRCLHRLL 525
BLAST of SMED30004411 vs. TrEMBL
Match: A0A430QGL0 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0008880 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 2.939e-10 Identity = 45/122 (36.89%), Postives = 66/122 (54.10%), Query Frame = 1 Query: 1072 IKSCPDWCSTVETECPYFTPID--GNGGEPAFLCDESHYHHNARYR-----LNTNASCGADMPWNHCCY--------STELLDETQSTSNYRFFSQFSPNSE--TNQKSCISEQKNCLYKLI 1386 ++ C WC+ VET CPY P D NGGEPAFLCDESHYHH++RY+ +NT C ++ CC+ S + D+ Q ++ F +S + E + SC + CL++L+ Sbjct: 412 VQPCISWCTQVETVCPYLNPADPTSNGGEPAFLCDESHYHHSSRYQAVYWDVNT---CESE-----CCFGVSDSILLSVSIPDKKQYSTPTDFLLSYSSDVEESNDNDSCSHLRDRCLHRLL 525
BLAST of SMED30004411 vs. TrEMBL
Match: G4VFV3 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_160820 PE=4 SV=1) HSP 1 Score: 73.9442 bits (180), Expect = 6.109e-10 Identity = 30/49 (61.22%), Postives = 37/49 (75.51%), Query Frame = 1 Query: 1063 LIGIKSCPDWCSTVETECPYFTPID--GNGGEPAFLCDESHYHHNARYR 1203 L+ ++ C WC+ VET CPY P D NGGEPAFLCDESHYHH++RY+ Sbjct: 447 LVIVQPCISWCTQVETVCPYLNPADPTSNGGEPAFLCDESHYHHSSRYQ 495
BLAST of SMED30004411 vs. Planmine SMEST
Match: SMESG000061558.1 (SMESG000061558.1) HSP 1 Score: 780.785 bits (2015), Expect = 0.000e+0 Identity = 495/496 (99.80%), Postives = 495/496 (99.80%), Query Frame = 1 Query: 82 MTNFKSDHNGYQKIPINQLKLITCNXXXXXXXXXXXXXXXXPCNICFILLFIIQISSTGLMLISVGLSFKMISAEVQLVNQSSKYIKNLILNSGNISTTNQTNNIETNXXXXXXXXXXXXXXXXXXPTDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYIXXXXXXXXXXXXXXXXXXXXKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYRFFSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVXXXXXXXXXXXXXXXXXXXXXHAVSISELXXXXXXXXXXXXXXXXXXXXXNFSIR 1569 MTNFKSDHNGYQKIPINQLKLITCNYLYNSSSSISNFKKLFPCNICFILLFIIQISSTGLMLISVGLSFKMISAEVQLVNQSSKYIKNLILNSGNISTTNQTNNIETNRDFYRLFLRRSRRLSDDLPTDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYISLTLPNSNSNSDSSESANLNKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYR FSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVNNSLGNNNNNENSFQNNPSNSHAVSISELSNNQLQIMISISSSFFLYFYMNFSIR Sbjct: 1 MTNFKSDHNGYQKIPINQLKLITCNYLYNSSSSISNFKKLFPCNICFILLFIIQISSTGLMLISVGLSFKMISAEVQLVNQSSKYIKNLILNSGNISTTNQTNNIETNRDFYRLFLRRSRRLSDDLPTDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYISLTLPNSNSNSDSSESANLNKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYRIFSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVNNSLGNNNNNENSFQNNPSNSHAVSISELSNNQLQIMISISSSFFLYFYMNFSIR 496
BLAST of SMED30004411 vs. Planmine SMEST
Match: SMESG000061558.1 (SMESG000061558.1) HSP 1 Score: 739.184 bits (1907), Expect = 0.000e+0 Identity = 479/496 (96.57%), Postives = 479/496 (96.57%), Query Frame = 1 Query: 82 MTNFKSDHNGYQKIPINQLKLITCNXXXXXXXXXXXXXXXXPCNICFILLFIIQISSTGLMLISVGLSFKMISAEVQLVNQSSKYIKNLILNSGNISTTNQTNNIETNXXXXXXXXXXXXXXXXXXPTDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYIXXXXXXXXXXXXXXXXXXXXKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYRFFSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVXXXXXXXXXXXXXXXXXXXXXHAVSISELXXXXXXXXXXXXXXXXXXXXXNFSIR 1569 MTNFKSDHNGYQKIPINQLKLITCNYLYNSSSSISNFKKLFPCNICFILLFIIQISST EVQLVNQSSKYIKNLILNSGNISTTNQTNNIETNRDFYRLFLRRSRRLSDDLPTDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYISLTLPNSNSNSDSSESANLNKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYR FSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVNNSLGNNNNNENSFQNNPSNSHAVSISELSNNQLQIMISISSSFFLYFYMNFSIR Sbjct: 1 MTNFKSDHNGYQKIPINQLKLITCNYLYNSSSSISNFKKLFPCNICFILLFIIQISST----------------EVQLVNQSSKYIKNLILNSGNISTTNQTNNIETNRDFYRLFLRRSRRLSDDLPTDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYISLTLPNSNSNSDSSESANLNKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYRIFSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVNNSLGNNNNNENSFQNNPSNSHAVSISELSNNQLQIMISISSSFFLYFYMNFSIR 480
BLAST of SMED30004411 vs. Planmine SMEST
Match: SMESG000061558.1 (SMESG000061558.1) HSP 1 Score: 518.079 bits (1333), Expect = 0.000e+0 Identity = 321/322 (99.69%), Postives = 321/322 (99.69%), Query Frame = 1 Query: 604 MLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYIXXXXXXXXXXXXXXXXXXXXKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYRFFSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVXXXXXXXXXXXXXXXXXXXXXHAVSISELXXXXXXXXXXXXXXXXXXXXXNFSIR 1569 MLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYISLTLPNSNSNSDSSESANLNKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYR FSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVNNSLGNNNNNENSFQNNPSNSHAVSISELSNNQLQIMISISSSFFLYFYMNFSIR Sbjct: 1 MLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKTEVRYSSVIFCEDCEMAYKKWLCTNQFDIYISLTLPNSNSNSDSSESANLNKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYSTELLDETQSTSNYRIFSQFSPNSETNQKSCISEQKNCLYKLIQSFYLVNNSLGNNNNNENSFQNNPSNSHAVSISELSNNQLQIMISISSSFFLYFYMNFSIR 322
BLAST of SMED30004411 vs. Planmine SMEST
Match: SMESG000041479.1 (SMESG000041479.1) HSP 1 Score: 78.9518 bits (193), Expect = 9.849e-16 Identity = 68/267 (25.47%), Postives = 123/267 (46.07%), Query Frame = 1 Query: 463 TDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKT-EVRYSSVIFCEDCEMAYKKWLCTNQFDIYIXXXXXXXXXXXXXXXXXXXXKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYS 1260 T+FS T R IC + F L+ +L +C + +S VI++ + + + + SS+ F L++ L+ ++N K + +I + +++ C +++ L+E D + + +Q+F + + + + T E + + + C YK WLC+ F + + + S ++ E Q + + C C+ VET+CPYF P+ NGGEP FLCD +HYH A L + C + +CC S Sbjct: 23 TNFSQFATDQIARQICDFPNKCATFHILNNFQLGFCHL-KLSDVIWTSVWNNVQNL-TSSIRYFHRCTNLLYE-----LQFIDNSLNGK-TILVSDLIMEYINSKSKCNSIIMGLIELDEQARQYLQQFQDIVTVHQSYHVTYEAKSTKEVQFRQCLKTYKNWLCSELFSKFALVDKNYTFSGNEDPEF--------RQIKYVKLPKCARKCAQVETDCPYFQPLIQNGGEPGFLCDSNHYHSGAMESLMKSEQC---ITTRYCCKS 270
BLAST of SMED30004411 vs. Planmine SMEST
Match: SMESG000041479.1 (SMESG000041479.1) HSP 1 Score: 79.337 bits (194), Expect = 1.103e-15 Identity = 68/267 (25.47%), Postives = 123/267 (46.07%), Query Frame = 1 Query: 463 TDFSLVLTRDNIRSICGTNSLVNRFEYLSRVKLRYCDIFHVSSVIYSMLVSEFETVNVSSLFPFDYINPSLHDIPTIMLRMLENGYIAKLNNSLHKIIDNHFSTEENCVNVLNKLLEEDNRIERDVQRFVESLIKNNCSNKT-EVRYSSVIFCEDCEMAYKKWLCTNQFDIYIXXXXXXXXXXXXXXXXXXXXKNSKTQSALIGIKSCPDWCSTVETECPYFTPIDGNGGEPAFLCDESHYHHNARYRLNTNASCGADMPWNHCCYS 1260 T+FS T R IC + F L+ +L +C + +S VI++ + + + + SS+ F L++ L+ ++N K + +I + +++ C +++ L+E D + + +Q+F + + + + T E + + + C YK WLC+ F + + + S ++ E Q + + C C+ VET+CPYF P+ NGGEP FLCD +HYH A L + C + +CC S Sbjct: 82 TNFSQFATDQIARQICDFPNKCATFHILNNFQLGFCHL-KLSDVIWTSVWNNVQNL-TSSIRYFHRCTNLLYE-----LQFIDNSLNGK-TILVSDLIMEYINSKSKCNSIIMGLIELDEQARQYLQQFQDIVTVHQSYHVTYEAKSTKEVQFRQCLKTYKNWLCSELFSKFALVDKNYTFSGNEDPEFR--------QIKYVKLPKCARKCAQVETDCPYFQPLIQNGGEPGFLCDSNHYHSGAMESLMKSEQC---ITTRYCCKS 329 The following BLAST results are available for this feature:
BLAST of SMED30004411 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004411 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004411 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30004411 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004411 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004411 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004411 ID=SMED30004411|Name=SMED30004411|organism=Schmidtea mediterranea sexual|type=transcript|length=1814bpback to top protein sequence of SMED30004411-orf-1 >SMED30004411-orf-1 ID=SMED30004411-orf-1|Name=SMED30004411-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=497bp MTNFKSDHNGYQKIPINQLKLITCNYLYNSSSSISNFKKLFPCNICFILLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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