DNA polymerase

Overview
NameDNA polymerase
Smed IDSMED30004407
Length (bp)3559
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA polymerase (SMED30004407) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA polymerase (SMED30004407) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA polymerase (SMED30004407) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA polymerase (SMED30004407) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30004407

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30004407h1SMcG0006535 SmedASXL_017047SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30004407h1SMcG0006535 SmedASXL_017048SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30004407h1SMcG0006535 SmedASXL_017048SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30004407h1SMcG0006535 dd_Smed_v4_12966_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30004407h1SMcG0006535 dd_Smed_v6_12966_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
neoblastSMED30004407h1SMcG0006535 SmWIOct06_038736GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30004407h1SMcG0006535 SMED_07764_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30004407h1SMcG0006535 SMED_07764_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30004407h1SMcG0006535 SmWIOct06_028601GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30004407h1SMcG0006535 SmWIOct06_037845GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30004407h1SMcG0006535 SmWIOct06_019067GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30004407h1SMcG0006535 SmWIOct06_013095GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175])

HSP 1 Score: 1131.32 bits (2925), Expect = 0.000e+0
Identity = 537/906 (59.27%), Postives = 690/906 (76.16%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++TV +P LVA  +R+L +G+ +   G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y +R + EK + CQLE DV W  ++ H  E  W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ WS FIR +DPD+ITGYN QNFDLPYLI+RA  L V+ F  LGR+  + S I +S  QSKQ GRR  K V M GR+  D+  +LLR++KLRSY+LNAVSFHFL EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPLSYLLSRGQQ+KV+SQLLR+A  E  ++P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GLTED +I+TP G  FVK+SV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY   AKV+YGDTDSVMC FGVS+V EAMALG+EAA  +SG F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+   LR+LLI RD   A++HAQ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI +       Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K      VG L  +   +  CIGC+ VL   + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q++++ R  
Sbjct:  198 MFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLK-EKATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-TAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG 1100          

HSP 2 Score: 75.485 bits (184), Expect = 5.377e-13
Identity = 40/120 (33.33%), Postives = 58/120 (48.33%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRA-SQHQYAENLICKVEYCLKAS 547
            +W R     + P+  PLIFQQL+ID+Y      +    P S     V R +G    G S+  H+HGF PY Y   PPGF  EH G+ ++ LN  + + +    +     +  VE C + S
Sbjct:   78 RWLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRES 197          
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175])

HSP 1 Score: 1131.32 bits (2925), Expect = 0.000e+0
Identity = 537/906 (59.27%), Postives = 690/906 (76.16%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++TV +P LVA  +R+L +G+ +   G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y +R + EK + CQLE DV W  ++ H  E  W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ WS FIR +DPD+ITGYN QNFDLPYLI+RA  L V+ F  LGR+  + S I +S  QSKQ GRR  K V M GR+  D+  +LLR++KLRSY+LNAVSFHFL EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPLSYLLSRGQQ+KV+SQLLR+A  E  ++P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GLTED +I+TP G  FVK+SV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY   AKV+YGDTDSVMC FGVS+V EAMALG+EAA  +SG F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+   LR+LLI RD   A++HAQ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI +       Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K      VG L  +   +  CIGC+ VL   + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q++++ R  
Sbjct:  198 MFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLK-EKATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-TAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG 1100          

HSP 2 Score: 75.485 bits (184), Expect = 5.377e-13
Identity = 40/120 (33.33%), Postives = 58/120 (48.33%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRA-SQHQYAENLICKVEYCLKAS 547
            +W R     + P+  PLIFQQL+ID+Y      +    P S     V R +G    G S+  H+HGF PY Y   PPGF  EH G+ ++ LN  + + +    +     +  VE C + S
Sbjct:   78 RWLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRES 197          
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175])

HSP 1 Score: 1131.32 bits (2925), Expect = 0.000e+0
Identity = 537/906 (59.27%), Postives = 690/906 (76.16%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++TV +P LVA  +R+L +G+ +   G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y +R + EK + CQLE DV W  ++ H  E  W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ WS FIR +DPD+ITGYN QNFDLPYLI+RA  L V+ F  LGR+  + S I +S  QSKQ GRR  K V M GR+  D+  +LLR++KLRSY+LNAVSFHFL EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPLSYLLSRGQQ+KV+SQLLR+A  E  ++P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GLTED +I+TP G  FVK+SV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY   AKV+YGDTDSVMC FGVS+V EAMALG+EAA  +SG F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+   LR+LLI RD   A++HAQ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI +       Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K      VG L  +   +  CIGC+ VL   + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q++++ R  
Sbjct:  198 MFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLK-EKATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-TAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG 1100          

HSP 2 Score: 75.485 bits (184), Expect = 5.377e-13
Identity = 40/120 (33.33%), Postives = 58/120 (48.33%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRA-SQHQYAENLICKVEYCLKAS 547
            +W R     + P+  PLIFQQL+ID+Y      +    P S     V R +G    G S+  H+HGF PY Y   PPGF  EH G+ ++ LN  + + +    +     +  VE C + S
Sbjct:   78 RWLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRES 197          
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175])

HSP 1 Score: 1117.45 bits (2889), Expect = 0.000e+0
Identity = 537/932 (57.62%), Postives = 690/932 (74.03%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKG--------------------------FYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++TV +P LVA  +R+L +G+ +   G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y +R + EK + CQLE DV W  ++ H  E  W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ WS FIR +DPD+ITGYN QNFDLPYLI+RA  L V+ F  LGR+  + S I +S  QSKQ GRR  K V M GR+  D+  +LLR++KLRSY+LNAVSFHFL EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPLSYLLSRGQQ+KV+SQLLR+A  E  ++P  +S+ G+++TGATVIEP+KG                          +YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GLTED +I+TP G  FVK+SV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY   AKV+YGDTDSVMC FGVS+V EAMALG+EAA  +SG F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+   LR+LLI RD   A++HAQ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI +       Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K      VG L  +   +  CIGC+ VL   + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q++++ R  
Sbjct:  198 MFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLK-EKATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGVRPQDRAGAAWELLALTPGRGCSPPRYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-TAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG 1126          

HSP 2 Score: 75.485 bits (184), Expect = 4.901e-13
Identity = 40/120 (33.33%), Postives = 58/120 (48.33%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRA-SQHQYAENLICKVEYCLKAS 547
            +W R     + P+  PLIFQQL+ID+Y      +    P S     V R +G    G S+  H+HGF PY Y   PPGF  EH G+ ++ LN  + + +    +     +  VE C + S
Sbjct:   78 RWLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRES 197          
BLAST of DNA polymerase vs. Ensembl Human
Match: POLD1 (DNA polymerase delta 1, catalytic subunit [Source:HGNC Symbol;Acc:HGNC:9175])

HSP 1 Score: 1076.62 bits (2783), Expect = 0.000e+0
Identity = 518/906 (57.17%), Postives = 669/906 (73.84%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++TV +P LVA  +R+L +G+ +   G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y +R + EK + CQLE DV W  ++ H  E  W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ WS FIR +DPD+ITGYN QNFDLPYLI+RA  L V+ F  LGR+  + S I +S  QSKQ GRR  K V M GR+  D+  +LLR++KLRSY+LNAVSFHFL EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPLSYLLSRGQQ+KV+SQLLR+A  E  ++P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GLTED +I+TP G  FVK+SV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK                        SVT FGR+MI+ TK++VES +   NGY   AKV+YGDTDSVMC FGVS+V EAMALG+EAA  +SG F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+   LR+LLI RD   A++HAQ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI +       Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K      VG L  +   +  CIGC+ VL   + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q++++ R  
Sbjct:  198 MFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLK-EKATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-TAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALK------------------------SVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG 1076          

HSP 2 Score: 75.485 bits (184), Expect = 4.813e-13
Identity = 40/120 (33.33%), Postives = 58/120 (48.33%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRA-SQHQYAENLICKVEYCLKAS 547
            +W R     + P+  PLIFQQL+ID+Y      +    P S     V R +G    G S+  H+HGF PY Y   PPGF  EH G+ ++ LN  + + +    +     +  VE C + S
Sbjct:   78 RWLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRES 197          
BLAST of DNA polymerase vs. Ensembl Celegans
Match: F10C2.4 (DNA polymerase delta catalytic subunit [Source:UniProtKB/Swiss-Prot;Acc:P90829])

HSP 1 Score: 989.564 bits (2557), Expect = 0.000e+0
Identity = 494/915 (53.99%), Postives = 644/915 (70.38%), Query Frame = 3
Query:  726 DIYGF-SQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLAS-FDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLP--DYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKA--NGYV-HDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVL--RDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++Y F   + K  F+KV+    +L    +  L  G+ +   G  N+ + ++++I+  V F+  + + GC WIE+P     +   +EK S CQ+EV V    +I H ++ EW  IAPIR LS DIEC GR+G FP+A  DP+IQI+N++   GE+EPF++N F L TCAP+VG+++I    E  LLE W+ F+RE+DPDIITGYN  NFDLPY+++RA  L++   S LGR K   S + ++   SKQMG R+NK++ + GRI FD+  ++LRD+KLRSY+LN+VS+ FL EQKEDV H II DLQ GD++TR RLA YCLKD+ L LRL++KLM ++NY+EMARVTGVP+++LL++GQQIK+LS +LR+    +F LP  +  S DG+ + GATVI+PI+GFY++PIATLDFASLYPSIM+AHNLCYTTLLKS +     G+  +DYI+TP G YF   S ++GLLP IL  ++ ARK+AK ++  E D FKR V +GRQLALKISANSVYGFTGA  G+LPCL IS SVTAFGR+MID TK  VE  + K   +G    DAKVIYGDTDSVM  FGV TV +AM +G +AA+ +S  F  PIKLEFEKVY PYLLI+KKRYAG+Y+TKPD  DKMDCKG+ETVRRDNCPLVA +L  CL KLLI RD+ +AL  A+  I+DLLCN+IDIS LIISKELTK+ ++Y +KQ H ELA ++KKRD GSAP+LGDRVPYV          Y +AEDP +VL NNI +DT +YL NQL  PL RIFEPI G + A + L+ GEH   +   QS VG L+ +T     C+GCK+VL   + E ++C HC+P    I+         LE  F RLWTECQ C+ ++  +V CS RDCPI+Y R+KV+ EL     ++ER  
Sbjct:  165 NLYYFRGADTKVPFVKVSGSTEAL-HKARMELKNGVNLMGKGPVNVGNLYESNINVIVMFLAKTNIVGCGWIEIPAGKCRILSNSEKSSRCQIEVTVPVKNLIVHESDGEWAGIAPIRTLSLDIECIGRRGVFPEAIKDPIIQIANLVKIEGEAEPFVRNCFVLGTCAPVVGSNIIQCVNEKVLLEKWAEFVREVDPDIITGYNILNFDLPYILDRAKVLSLPQVSHLGRQKEKGSVVRDAAISSKQMGSRVNKSIDIHGRIIFDVLQVVLRDYKLRSYTLNSVSYQFLSEQKEDVEHNIIPDLQRGDEQTRRRLAQYCLKDAYLPLRLLDKLMSIINYIEMARVTGVPMNFLLTKGQQIKILSMMLRRCKQNNFFLPVIEANSGDGEGYEGATVIDPIRGFYNEPIATLDFASLYPSIMIAHNLCYTTLLKSPQ-----GVENEDYIRTPSGQYFATKSKRRGLLPEILEDILAARKRAKNDMKNEKDEFKRMVYNGRQLALKISANSVYGFTGATVGKLPCLEISQSVTAFGRKMIDMTKLEVERIYKKGALDGKCPADAKVIYGDTDSVMVKFGVETVAQAMEIGLDAAKEVSKIFTPPIKLEFEKVYSPYLLINKKRYAGLYFTKPDVHDKMDCKGLETVRRDNCPLVAKVLGVCLEKLLIERDQQSALDFAKRTISDLLCNKIDISLLIISKELTKSGDKYQAKQAHVELAARMKKRDAGSAPRLGDRVPYVFVAAAKNVPAYERAEDPTFVLQNNIPLDTKHYLTNQLAKPLARIFEPILG-DRAEKILVEGEHTRVRTVVQSKVGGLAAFTTKSATCLGCKSVLPRAESENAVCKHCEPKLPTIFASRMNTMHELENHFGRLWTECQNCAKTMQDKVNCSARDCPIYYMREKVRNELSEASAVIERFG 1072          
BLAST of DNA polymerase vs. Ensembl Celegans
Match: rev-3 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A0A0K3AX87])

HSP 1 Score: 228.794 bits (582), Expect = 2.283e-61
Identity = 179/631 (28.37%), Postives = 309/631 (48.97%), Query Frame = 3
Query: 1281 SNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRT-RHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPD----YQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLL-------------KSKESIR----EFGLTEDDYIK---------TPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYT-------KAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPI 3056
            + +I + D D++ GY T      +   R          LLG   SM   + ++     ++  +        GR+   ++ ++  D  LR+Y L +   + L ++   + +  +     G+    R+ + ++ LK S L + L+ ++   +   EMARV G+    + +RG Q++V S LLR A   +FV P      ++  G       ++EP    Y  P+  LDF SLYPS+++A+N CY+T+L             +++E I     ++  ++DD +K         +P  S FVK S ++G++P +L +++ AR   K  +    +   +++LD RQLALK+ AN  YG+T A   GR+PC  ++ ++   GRE ++ + EMV+       G    A+VIYGDTDS+  L   ++V EA  +G+     ++     P+ L+ EKVY   +L +KKRYAG  Y   +    +D KG+ETVRRD CP+VA +L   L  L+ S++    +++  +V+ +L   + + S+ +  KE  + D    +  P  ++A+   ++ P      G+RVPYVI        +Y+        A +P Y       I+T YYL   +   L R+ + I
Sbjct:  307 AKWIVQYDVDVMIGYETIRLSWGFFFRR--------IKLLGSRISMDRALIDAYEDHIEVDDQEITVAPPKGRLLVSVWKVVRSDLALRNYDLGSAVANVLRKKIPMLDNAALMRRIKGERSAIRNDVHLHLLKLSSLNISLLTEMNWFLKNAEMARVYGIQFHEVWTRGSQLRVESMLLRLAHRMNFVAPSITHLQRNMMGSPEQLQLILEPQSKVYFDPVIVLDFQSLYPSMVIAYNYCYSTILGKIGNLVQMNDESRNREEIVLGAIKYHPSKDDIVKLVAYKEVCASPLASMFVKKSKREGVMPLLLREILAARIMVKSAMKRTKNKKLKRILDARQLALKLVANVSYGYTAANWSGRMPCAELADAILGKGRETLERSIEMVQ------RGDYGGAEVIYGDTDSMFVLVRGASVEEAFEIGRRIVDDVTNSNPDPVVLKLEKVYKGCVLETKKRYAGWMYEHENDEGSLDAKGIETVRRDTCPIVAEVLEKSL-GLIFSQNWKTFITYLNTVVLNL--PQENFSKFVFCKEY-RGDYSARAMVPQKKIAEARIRKCPSHITLRGERVPYVIIDGVTGSTVYSCVRSIDQFARNPEY------KINTFYYLNAHILAALRRVTDLI 913          
BLAST of DNA polymerase vs. Ensembl Celegans
Match: rev-3 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A0A0K3AX87])

HSP 1 Score: 229.18 bits (583), Expect = 6.827e-61
Identity = 179/631 (28.37%), Postives = 309/631 (48.97%), Query Frame = 3
Query: 1281 SNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRT-RHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPD----YQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLL-------------KSKESIR----EFGLTEDDYIK---------TPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYT-------KAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPI 3056
            + +I + D D++ GY T      +   R          LLG   SM   + ++     ++  +        GR+   ++ ++  D  LR+Y L +   + L ++   + +  +     G+    R+ + ++ LK S L + L+ ++   +   EMARV G+    + +RG Q++V S LLR A   +FV P      ++  G       ++EP    Y  P+  LDF SLYPS+++A+N CY+T+L             +++E I     ++  ++DD +K         +P  S FVK S ++G++P +L +++ AR   K  +    +   +++LD RQLALK+ AN  YG+T A   GR+PC  ++ ++   GRE ++ + EMV+       G    A+VIYGDTDS+  L   ++V EA  +G+     ++     P+ L+ EKVY   +L +KKRYAG  Y   +    +D KG+ETVRRD CP+VA +L   L  L+ S++    +++  +V+ +L   + + S+ +  KE  + D    +  P  ++A+   ++ P      G+RVPYVI        +Y+        A +P Y       I+T YYL   +   L R+ + I
Sbjct:  591 AKWIVQYDVDVMIGYETIRLSWGFFFRR--------IKLLGSRISMDRALIDAYEDHIEVDDQEITVAPPKGRLLVSVWKVVRSDLALRNYDLGSAVANVLRKKIPMLDNAALMRRIKGERSAIRNDVHLHLLKLSSLNISLLTEMNWFLKNAEMARVYGIQFHEVWTRGSQLRVESMLLRLAHRMNFVAPSITHLQRNMMGSPEQLQLILEPQSKVYFDPVIVLDFQSLYPSMVIAYNYCYSTILGKIGNLVQMNDESRNREEIVLGAIKYHPSKDDIVKLVAYKEVCASPLASMFVKKSKREGVMPLLLREILAARIMVKSAMKRTKNKKLKRILDARQLALKLVANVSYGYTAANWSGRMPCAELADAILGKGRETLERSIEMVQ------RGDYGGAEVIYGDTDSMFVLVRGASVEEAFEIGRRIVDDVTNSNPDPVVLKLEKVYKGCVLETKKRYAGWMYEHENDEGSLDAKGIETVRRDTCPIVAEVLEKSL-GLIFSQNWKTFITYLNTVVLNL--PQENFSKFVFCKEY-RGDYSARAMVPQKKIAEARIRKCPSHITLRGERVPYVIIDGVTGSTVYSCVRSIDQFARNPEY------KINTFYYLNAHILAALRRVTDLI 1197          
BLAST of DNA polymerase vs. Ensembl Celegans
Match: pola-1 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:C7IVS4])

HSP 1 Score: 182.956 bits (463), Expect = 2.323e-46
Identity = 212/791 (26.80%), Postives = 359/791 (45.39%), Query Frame = 3
Query:  864 SFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKI----ICHSTEEWPSIAPIRILS-------------------FDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPF-IKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRS----KITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPD-----------YQSKDGDE-------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDS-FDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQ--SVIADLL--------CNRIDISQLIISKELTKTDEEYTS--KQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNN-IGIDTDYYLKNQLTNPLLRIFEPI 3056
            +F+T +    R +++    G  WIEL    Y       K S C+ E +V  +K+       S++  P    +  L+                   F+ +C     S    D+ P   ++      G S P+ I+        +  V +       E  LL  +   I E +PD+I G++  +  +  L++R  KL +  +S + R+K  RS    KI  S+S   ++           GR+  D  L  +   K RS+ L  +S   L  ++ ++    I  L   D +    L  +   D  L LR++ +L  +  Y++++++ G   S  +  G+  +    LL      D + PD            +S++GDE       ++G  V+EP KG Y+  I  LDF SLYPSI+  +N+CYTTL  SK+S        D+ +  P      +S+  +G+LP  + +L+E R+  K  +  E +  K++ +D RQ+ALK++ANS+YG  G Q  R     +++ VTA GRE++  +K++VE       GY     V+YGDTDS+M       +V A  LG E  ++++ K    ++L+ + V +  +L+ KK+        PD+  +  + KG++ VRRD   L        + K+L     +A+L+  +  S I DLL           + +    ISK+LT+  E+Y     Q H+ +AQ++ K    +  +  D V YVI   G  +    +A     + +N+ + ID  YYL  Q+   + R+ EPI
Sbjct:  457 TFNTSVTPLERLLIEKKFMGPGWIEL----YNYSDPKAKTSNCKYEFEVDMEKMRNIKYLESSDAPPPTIKLLALNVVTTLNDRKDNEICMISMLFNPKCDLAHPSGDSKDLKPKCLVTKPP---GGSLPYDIQKRLETEKLSKFVKSVA----NEKALLTLFLAMINEDEPDMIVGHDL-SATIALLVSRLEKLKLPNWSRISRLK--RSINIGKIGHSKSGQWEL---------TAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLY-VDSKDLISLINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDS--------DEQLSVP------QSTDIEGVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEKM-----GY----SVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVN-KCHRLLELDLDGV-FKRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKIL-----DASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSGKFNL-RHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPI 1192          
BLAST of DNA polymerase vs. Ensembl Celegans
Match: pola-1 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:C7IVS4])

HSP 1 Score: 182.956 bits (463), Expect = 2.484e-46
Identity = 212/791 (26.80%), Postives = 359/791 (45.39%), Query Frame = 3
Query:  864 SFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKI----ICHSTEEWPSIAPIRILS-------------------FDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPF-IKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRS----KITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPD-----------YQSKDGDE-------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDS-FDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQ--SVIADLL--------CNRIDISQLIISKELTKTDEEYTS--KQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNN-IGIDTDYYLKNQLTNPLLRIFEPI 3056
            +F+T +    R +++    G  WIEL    Y       K S C+ E +V  +K+       S++  P    +  L+                   F+ +C     S    D+ P   ++      G S P+ I+        +  V +       E  LL  +   I E +PD+I G++  +  +  L++R  KL +  +S + R+K  RS    KI  S+S   ++           GR+  D  L  +   K RS+ L  +S   L  ++ ++    I  L   D +    L  +   D  L LR++ +L  +  Y++++++ G   S  +  G+  +    LL      D + PD            +S++GDE       ++G  V+EP KG Y+  I  LDF SLYPSI+  +N+CYTTL  SK+S        D+ +  P      +S+  +G+LP  + +L+E R+  K  +  E +  K++ +D RQ+ALK++ANS+YG  G Q  R     +++ VTA GRE++  +K++VE       GY     V+YGDTDS+M       +V A  LG E  ++++ K    ++L+ + V +  +L+ KK+        PD+  +  + KG++ VRRD   L        + K+L     +A+L+  +  S I DLL           + +    ISK+LT+  E+Y     Q H+ +AQ++ K    +  +  D V YVI   G  +    +A     + +N+ + ID  YYL  Q+   + R+ EPI
Sbjct:  457 TFNTSVTPLERLLIEKKFMGPGWIEL----YNYSDPKAKTSNCKYEFEVDMEKMRNIKYLESSDAPPPTIKLLALNVVTTLNDRKDNEICMISMLFNPKCDLAHPSGDSKDLKPKCLVTKPP---GGSLPYDIQKRLETEKLSKFVKSVA----NEKALLTLFLAMINEDEPDMIVGHDL-SATIALLVSRLEKLKLPNWSRISRLK--RSINIGKIGHSKSGQWEL---------TAGRMMLDSKLAAMELVKSRSFDLTELSQQILGAERREIYANEIPQLY-VDSKDLISLINWSWNDLLLSLRIVVRLNALPLYIQISQIVGGIASRTMMGGRAERNEYLLLHAFEKADLIAPDKYNSSESKKKKEESQEGDEKKSGKAQYSGGLVLEPKKGLYETLILLLDFNSLYPSIIQEYNICYTTLEYSKDS--------DEQLSVP------QSTDIEGVLPREIRKLVECRRDVKSLMKSERNEAKKKQMDIRQMALKLTANSMYGCLGFQYSRFYAKPLAALVTAKGREILMHSKDLVEKM-----GY----SVVYGDTDSIMINTNSVDLVAAKKLGNEIKKAVN-KCHRLLELDLDGV-FKRMLLLKKKKYAALTINPDTKVEAKELKGLDIVRRDWSQLAKETGTAVVDKIL-----DASLTRDEMVSSIDDLLREIRAKLDSGTVPLEMFQISKQLTRNPEQYADVKAQCHAAVAQRLNKSGKFNL-RHNDIVEYVICEDGTDNPATQRAYHRTEMAENSALKIDLLYYLAQQIHPVVSRLVEPI 1192          
BLAST of DNA polymerase vs. Ensembl Fly
Match: DNApol-delta (gene:FBgn0263600 transcript:FBtr0075522)

HSP 1 Score: 1135.94 bits (2937), Expect = 0.000e+0
Identity = 545/915 (59.56%), Postives = 700/915 (76.50%), Query Frame = 3
Query:  723 LDIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKI---SFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDE-FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLL--KSKESIREFGLTEDDYI-KTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRD--DEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERL 3437
            L+I+G++ + K+ ++K++V +P  VA   R+L + + +    + +  +F+ +IDF +RFMVD+ + GC+WIELP+  + +R    K    S CQ+EVDV +D+ I H  E EW  +AP RILSFDIECAGRKG FP+A IDPVIQI+N+++R GE EPFI+N+FTLN CAPI+G+ V+ H  E ++L+ WS F+RE+DPDI+TGYN  NFD PYL+NRA  L V+ F  LGRIK++RS I E   QSKQMGRR N+ V+  GR+ FDL  +LLRD+KLRSY+LNAVS+HFL+EQKEDV H IITDLQNGD++TR RLA+YCLKD+ L LRL+ KLM +VNY+EMARVTGVPL  LL+RGQQIKVLSQLLRKA  + F++P Y S+  DE + GATVIEP +G+Y  PI+TLDFASLYPSIMMAHNLCYTTL+   ++E +R+    +DD + +TP  +YFVKS V++GLLP IL  L+ ARK+AK +L +ETDPFKR+VLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVTA+GR MI+ TK  VES + +ANGY ++A VIYGDTDSVM  FGV T+  +M LG+EAA+ +S KF+ PIKLEFEKVYYPYLLI+KKRYAG+Y+T+PD++DKMDCKG+ETVRRDN PLVANL+N+CL+KLLI RD + A+++ + VIADLLCNRIDIS L+I+KEL KTD  Y +KQ H ELA K+KKRDPG+AP+LGDRVPYVI         Y KAEDP+YVL+N++ ID  YYL+ QL+ PLLRIFEPI G   A   LL GEH  ++    S VG L+ +   K  C+GCK+++    ++  +C HC+P    +Y +E    + LE  FSRLWTECQRC  SLH+EV+CSNRDCPIFY R+KV+ +L +Q++ + R 
Sbjct:  176 LNIHGYNGDKKQRYIKISVTLPRFVAAASRLLKKEVIMSEIDFQDCRAFENNIDFDIRFMVDTDVVGCNWIELPMGHWRIRNSHSKPLPESRCQIEVDVAFDRFISHEPEGEWSKVAPFRILSFDIECAGRKGIFPEAKIDPVIQIANMVIRQGEREPFIRNVFTLNECAPIIGSQVLCHDKETQMLDKWSAFVREVDPDILTGYNINNFDFPYLLNRAAHLKVRNFEYLGRIKNIRSVIKEQMLQSKQMGRRENQYVNFEGRVPFDLLFVLLRDYKLRSYTLNAVSYHFLQEQKEDVHHSIITDLQNGDEQTRRRLAMYCLKDAYLPLRLLEKLMAIVNYMEMARVTGVPLESLLTRGQQIKVLSQLLRKAKTKGFIMPSYTSQGSDEQYEGATVIEPKRGYYADPISTLDFASLYPSIMMAHNLCYTTLVLGGTREKLRQQENLQDDQVERTPANNYFVKSEVRRGLLPEILESLLAARKRAKNDLKVETDPFKRKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISGSVTAYGRTMIEMTKNEVESHYTQANGYENNAVVIYGDTDSVMVNFGVKTLERSMELGREAAELVSSKFVHPIKLEFEKVYYPYLLINKKRYAGLYFTRPDTYDKMDCKGIETVRRDNSPLVANLMNSCLQKLLIERDPDGAVAYVKQVIADLLCNRIDISHLVITKELAKTD--YAAKQAHVELAAKMKKRDPGTAPKLGDRVPYVICAAAKNTPAYQKAEDPLYVLENSVPIDATYYLEQQLSKPLLRIFEPILGDN-AESILLKGEHTRTRTVVTSKVGGLAGFMTKKTSCLGCKSLMPKGYEQACLCPHCEPRMSELYQKEVGAKRELEETFSRLWTECQRCQESLHEEVICSNRDCPIFYMRQKVRMDLDNQEKRVLRF 1087          

HSP 2 Score: 69.3218 bits (168), Expect = 2.111e-11
Identity = 34/91 (37.36%), Postives = 48/91 (52.75%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNYEIK--------ETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ 469
            +W+R     + P    L FQQLD++NY  +        +  P  V R +G    GNS+  HVHGF PY Y+E P  F + HC + +K L+Q
Sbjct:   60 RWSRPPPPELDPSKHNLEFQQLDVENYLGQPLPGMPGAQIGPVPVVRMFGVTMEGNSVCCHVHGFCPYFYIEAPSQFEEHHCEKLQKALDQ 150          
BLAST of DNA polymerase vs. Ensembl Fly
Match: DNApol-zeta (gene:FBgn0002891 transcript:FBtr0088831)

HSP 1 Score: 280.796 bits (717), Expect = 6.757e-77
Identity = 226/756 (29.89%), Postives = 361/756 (47.75%), Query Frame = 3
Query: 1254 TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFV--LPDYQSKD---GDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIR-----EFG-------------LTEDDYIK-TPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQ-VLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDE-EYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDN-NIGIDTDYYLKNQLTNPLLRIFEPIFGKELA------TRKLLHGEHVLSKFKQQSNVGA---LSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKEL 3407
            +EAE  E         D DI  GY  +     Y+I+RA  L      LL R+ +   K+ +   + ++    ++  + + GRI  D++ ++  +  L SY+   V +H L ++      + +T+   G   TR  +  Y L+     L L+++L ++    EMA++ G+    +LSRG Q +V S +LR A  ++ V   P  Q++      E+  A ++EP   FY  P+  LDF SLYPS+++A+N C++T L   E +      EFG             L E D +  +PCG  FVK  V++G+LP +L ++++ R+  K+ + +  D    Q +L  RQL LK+ AN  YG+T A   GR+P + +  SV + GRE ++   ++VE      N      +V+YGDTDS+  L       EA  +G+E A++++     P+KL+ EKVY P +L +KKRY G  Y   D    + + KG+ETVRRD CP VA +L   LR L  ++D +   ++       LL  R ++  LI +KE    +  + T+  P  EL +K  ++DP   P+ G+RVP++I       ++      P  +L N    I+  YY+   +  PL R    + G  +        RKLL    V +  +     GA   +S+Y       I C    R  +  IC  C  N        S     LE  +      CQ C G L   + C + DCP+ Y  +  ++EL
Sbjct: 1376 SEAEAFEALLALCERWDADIYAGYEIEMSSWGYVIDRAKHLCFNIAPLLSRVPTQ--KVRDFVDEDREQFTDLDVEMKLCGRILLDVWRLMRSEIALTSYTFENVMYHILHKRCPWHTAKSLTEW-FGSPCTRWIVMEYYLERVRGTLTLLDQLDLLGRTSEMAKLIGIQFYEVLSRGSQFRVESMMLRIAKPKNLVPLSPSVQARAHMRAPEYL-ALIMEPQSRFYADPLIVLDFQSLYPSMIIAYNYCFSTCLGRVEHLGGSSPFEFGASQLRVSRQMLQKLLEHDLVTVSPCGVVFVKREVREGILPRMLTEILDTRQMVKQSMKLHKDSSALQRILHSRQLGLKLMANVTYGYTAANFSGRMPSVEVGDSVVSKGRETLERAIKLVE------NNEEWKVRVVYGDTDSMFVLVPGRNRAEAFRIGEEIAKAVTEMNPQPVKLKLEKVYQPCMLQTKKRYVGYMYETADQEQPVYEAKGIETVRRDGCPAVAKMLEKVLRILFETQDVSKIKAYVCRQFTKLLSGRANLQDLIFAKEFRGLNGYKPTACVPALELTRKWMQKDPRHVPRRGERVPFIIVNGPPGMQLIRLVRSPHDILANEGHKINAIYYITKAIIPPLNRCLL-LIGANVHDWFASLPRKLLMTPAVGTANELAGARGAKSTISQYFSTTSCVIDCG---RQTKAGICPDCLKNATTCVVVLSDKTARLERGYQLTRQICQACCGRLGS-LQCDSLDCPVLYVLEGKRREL 2116          
BLAST of DNA polymerase vs. Ensembl Fly
Match: DNApol-alpha180 (gene:FBgn0259113 transcript:FBtr0084115)

HSP 1 Score: 169.859 bits (429), Expect = 3.335e-42
Identity = 195/821 (23.75%), Postives = 370/821 (45.07%), Query Frame = 3
Query:  780 PVPSLVATTKRVLVEGMTIPHYGYHNLAS-FDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTEEWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNII-VRFGESEPFIKNIFTLNTCA---PIV---------------GAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLE----EQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPD-----------------------YQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAI-ETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFD---KMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALS--HAQ--SVIADLLCNRIDISQLIISKELTKTDEEY--TSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKA---EDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFG 3062
            P+P+L A  K             Y+++A  F    +   RF++D  + G  W++  +  + V P    +S+C  EV +   K +    ++     P  +    +    R    P    + +  IS +   RF    P  +  F  + CA   P V                  V  H +E  LL  +    ++ID D+I  +++ +  L  + ++   L +  +S +GR++            S+  G+R+ +  H  GR+  D+        + RSY L  +    L+    E+ E  A  ++   + G+  T+  L    ++D+  +LRLM +L ++   +++  + G  ++  L  G+  +    LL     +++++PD                        Q+K    + G  V+EP++G Y+K +  +DF SLYPSI+  +N+C+TT+ +  ++        D+    P       S  + G+LP  L +L+E+RK+ K+ +A  +  P  +     RQ+ALK++ANS+YG  G    R    ++++ VT  GRE++  T+++V+           +  V+YGDTDS+M    ++   +   +G    QS++ K    ++L+ + V+   LL+ KK+YA +  +K    +   + + KG++ VRRD   L   +    L ++L  +     L   HAQ   +   +    + +   +I+K+LT+T +EY  ++  PH ++A ++  R+     + GD V YVI   G  +    +A   ++     D  + +DT+YYL +Q+   + R+ E + G
Sbjct:  480 PLPNLSADKK-------------YNSIAHIFGATTNALERFLLDRKIKGPCWLQ--VTGFKVSPTP--MSWCNTEVTLTEPKNVELVQDKGKPAPPPPLTLLSLNV--RTSMNPKTSRNEICMISMLTHNRFHIDRPAPQPAFNRHMCALTRPAVVSWPLDLNFEMAKYKSTTVHKHDSERALLSWFLAQYQKIDADLIVTFDSMDCQLNVITDQIVALKIPQWSRMGRLRL-----------SQSFGKRLLE--HFVGRMVCDVKRSAEECIRARSYDLQTLCKQVLKLKESERMEVNADDLLEMYEKGESITK--LISLTMQDNSYLLRLMCELNIMPLALQITNICGNTMTRTLQGGRSERNEFLLLHAFHEKNYIVPDKKPVSKRSGAGDTDATLSGADATMQTKKKAAYAGGLVLEPMRGLYEKYVLLMDFNSLYPSIIQEYNICFTTVQQPVDA--------DELPTLP------DSKTEPGILPLQLKRLVESRKEVKKLMAAPDLSPELQMQYHIRQMALKLTANSMYGCLGFAHSRFFAQHLAALVTHKGREILTNTQQLVQK---------MNYDVVYGDTDSLMINTNITDYDQVYKIGHNIKQSVN-KLYKQLELDIDGVFGCLLLLKKKKYAAIKLSKDSKGNLRREQEHKGLDIVRRDWSQLAVMVGKAVLDEVLSEKPLEEKLDAVHAQLEKIKTQIAEGVVPLPLFVITKQLTRTPQEYANSASLPHVQVALRM-NRERNRRYKKGDMVDYVICLDGTTNAAMQRAYHLDELKTSEDKKLQLDTNYYLGHQIHPVVTRMVEVLEG 1241          
BLAST of DNA polymerase vs. Ensembl Fly
Match: DNApol-epsilon255 (gene:FBgn0264326 transcript:FBtr0084408)

HSP 1 Score: 52.373 bits (124), Expect = 3.135e-6
Identity = 54/171 (31.58%), Postives = 74/171 (43.27%), Query Frame = 3
Query:  954 YTVRPQTEKISFCQLEVDVQWDKIICHSTEEWPSIA----------PIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFG----ESEPFIKNI--FTLNTCAPIVGAHVI-PHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRAN--KLNVK---GFSLL 1400
            Y VR   +   FC      QW  I C S  E P+I           P+ +L+FDIE       FPDA  D V+ IS +I   G      E    N+  F         G  ++   + E +LL+ + + I E+ P II  YN   FD P++  RA    L++K   GFS L
Sbjct:  226 YHVRVSIDLRIFCG-----QWYNIRCRSGVELPTITCRPDILDRPEPV-VLAFDIETTKLPLKFPDAQTDQVMMISYMIDGQGYLITNREIISSNVDDFEYTPKPEFEGNFIVFNEENEMQLLQRFFDHIMEVRPHIIVTYNGDFFDWPFVETRAAVYDLDMKQEIGFSKL 390          
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-060429-1])

HSP 1 Score: 1153.66 bits (2983), Expect = 0.000e+0
Identity = 557/916 (60.81%), Postives = 695/916 (75.87%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRP-----QTE-----KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            +Y +  N    FL++T+ +P LVA  KR+L +G    ++   +  +F+ +IDF +RFMVDS + GC WIELP   Y +R      QT+     K+S CQ EVDV WD +I H  E EW  IAP+R+LSFDIECAGRKG FP+ ++DPVIQI++++ R GE EPFI+ +FTL +CA IVG+ ++    E  +L+ W+ F+R +DPDIITGYN QNFDLPYL+NRA  L V  F  LGR+   +S + +S  QSKQMGRR NK ++M GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ KLM V+NY+EMARVTGVPL+YLLSRGQQIKV+SQLLR+A  +D V+P  +++ G+++TGATVIEP KG+Y  PIATLDF+SLYPSIMMAHNLCYTTLL+ K  I + GL  DD+IKTP G  FVKSSV+KGLLP IL  L+ ARK+AK EL  ETDPFK+QVLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  DAKVIYGDTDSVM   GV+TV EAM  GKEAA+ +S  F+ PIKLEFEKVYYPYLLI+KKRYAG+Y+ +  +  DKMDCKG+ETVRRDNCPLVANL+NTCL+ +LI RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRD GSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL+ PLLRIFEPI G+  A   LL G+H   K    S VG L  +   +  CIGC+ VL+ D  ++C  CK  E  +Y +E  +  +LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q++++ R  
Sbjct:  191 MYNYHGNKPHDFLRITMAMPRLVAPAKRLLEQGFKFANFATQSYQAFEANIDFEIRFMVDSNVVGCCWIELPKGKYQLREERREGQTDSKYPGKVSLCQYEVDVAWDSLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEPEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKLMLQSWAEFMRTVDPDIITGYNIQNFDLPYLLNRAATLKVNMFPYLGRVWGCKSVLKDSSFQSKQMGRRENKTINMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYTGATVIEPEKGYYSVPIATLDFSSLYPSIMMAHNLCYTTLLQ-KSQIEKLGLGPDDFIKTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESRYTLDNGYQADAKVIYGDTDSVMVKLGVATVQEAMNQGKEAAEWVSSHFVPPIKLEFEKVYYPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQNILIDRDPDGAVTHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGKQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLSKPLLRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-VAVCDFCKKRESELYQKEIAHLSTLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLVSRFG 1104          

HSP 2 Score: 72.4034 bits (176), Expect = 3.237e-12
Identity = 42/135 (31.11%), Postives = 66/135 (48.89%), Query Frame = 2
Query:  116 DEDDVSMDEWNHEPEEICFEMESNTETVKETDEFS-----KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            +E+    DE + E E    E ++  + +   D FS     +W R +   + P+   LIFQQ+D+D Y          +++   P  + R +G   NGNS+  H+HGF PY YV  P GF   H  +F++ LN  +
Sbjct:   35 EEELALFDEMDMEVES--GEGQAGHDVIPVGDLFSTDLNPRWKRPHASPLHPKSDTLIFQQIDLDYYLGAAVAGMPGQVQGKVP--IVRMFGVTDNGNSVCCHIHGFAPYFYVPAPNGFTNAHLADFQRELNSVV 165          
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55])

HSP 1 Score: 312.768 bits (800), Expect = 4.446e-87
Identity = 229/751 (30.49%), Postives = 368/751 (49.00%), Query Frame = 3
Query: 1257 EAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDL--VLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDF--VLPDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIR-----EFGLT------------EDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKREL-AIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSK-QPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFEPI------FGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKEL 3407
            E EL E  SN IR+ DPDI+ GY  Q     YL+ RA+ L V     L R+    S+      +  + G      +H+ GRI  +++ I+  +  L +Y+   V+FH L ++    +H+ ++D     D   HR  V     S +   ++L+ +  ++    E+ARV G+   ++L+RG Q +V S +LR A   ++  V P  Q +           V+EP   FY   +  LDF SLYPSI++A+N CY+T L   E++      +FG               +D   +P G  FVKSSV++G+LP +L ++++ R   K+ + A   D    ++LD RQL LK+ AN  +G+T A   GR+PC+ +  S+    RE ++   +MV  +  K  G    A+V+YGDTDS+  L   +T  +A  +G E A++++     PIKL+FEKVY P +L +KKRY G  Y   D  + + D KG+ETVRRD CP V+ +L   L+ L  SRD +    + Q     ++  +  +  L  +KE   +         P  EL +++   D    P++G+RVPYVI        +      P+ VL D ++ ++  YY+  Q+  PL RIF  I      +  EL   +         + ++ +  G +S+Y      C  C  +    +  +CA C+     +     +  +  E +  +L   C+ CSGS  ++V C + DCPI Y   +V + L
Sbjct: 2214 EKELFEEVSNIIRKYDPDILVGYEVQMHSWGYLLQRASTLGVNLCQQLSRVPG-DSQQNHFIPEKDEYGAETMTEIHITGRIILNIWRIMKTEAALNNYTFENVAFHLLHQRYPLYSHRTLSDWF---DTHLHRWKVMDHYVSRVCGTVQLLQQQDIIGRTSELARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAAQCIPLVMEPESRFYSNSVIVLDFQSLYPSIVIAYNYCYSTCLGHIENLGTYDEFKFGCASLRVPPDLLYQLRNDITVSPNGVAFVKSSVRRGVLPRMLEEILKTRIMVKQSMKAYRNDKALSRLLDARQLGLKLIANVTFGYTSANFSGRMPCVEVGDSIVHKARETLERAIKMVNDT--KKWG----ARVVYGDTDSMFVLLKGATKEQAFKIGNEIAEAVTATNPKPIKLKFEKVYLPCILQTKKRYVGYMYESVDQENPVFDAKGIETVRRDGCPGVSKILERSLKLLFESRDISQVKQYVQRQCVKVVEGKASMQDLTFAKEYRGSGSYRPGACVPALELTRRMMAFDRRLEPRVGERVPYVIVYGSPGVPLIQLVRRPLEVLQDPSLRLNATYYITKQILPPLARIFNLIGVDVFSWYHELPRVQRAWSFTSSGRGEEGTKKGTISQYFTTL-HCPVCDELT---QLGVCARCRAEPQCVAVHLHQDLRLWESQQDQLLKICRSCSGSAERQVPCVSLDCPILYKLSRVNRNL 2950          
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55])

HSP 1 Score: 311.612 bits (797), Expect = 1.020e-86
Identity = 229/751 (30.49%), Postives = 368/751 (49.00%), Query Frame = 3
Query: 1257 EAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDL--VLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDF--VLPDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIR-----EFGLT------------EDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKREL-AIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSK-QPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFEPI------FGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKEL 3407
            E EL E  SN IR+ DPDI+ GY  Q     YL+ RA+ L V     L R+    S+      +  + G      +H+ GRI  +++ I+  +  L +Y+   V+FH L ++    +H+ ++D     D   HR  V     S +   ++L+ +  ++    E+ARV G+   ++L+RG Q +V S +LR A   ++  V P  Q +           V+EP   FY   +  LDF SLYPSI++A+N CY+T L   E++      +FG               +D   +P G  FVKSSV++G+LP +L ++++ R   K+ + A   D    ++LD RQL LK+ AN  +G+T A   GR+PC+ +  S+    RE ++   +MV  +  K  G    A+V+YGDTDS+  L   +T  +A  +G E A++++     PIKL+FEKVY P +L +KKRY G  Y   D  + + D KG+ETVRRD CP V+ +L   L+ L  SRD +    + Q     ++  +  +  L  +KE   +         P  EL +++   D    P++G+RVPYVI        +      P+ VL D ++ ++  YY+  Q+  PL RIF  I      +  EL   +         + ++ +  G +S+Y      C  C  +    +  +CA C+     +     +  +  E +  +L   C+ CSGS  ++V C + DCPI Y   +V + L
Sbjct: 2028 EKELFEEVSNIIRKYDPDILVGYEVQMHSWGYLLQRASTLGVNLCQQLSRVPG-DSQQNHFIPEKDEYGAETMTEIHITGRIILNIWRIMKTEAALNNYTFENVAFHLLHQRYPLYSHRTLSDWF---DTHLHRWKVMDHYVSRVCGTVQLLQQQDIIGRTSELARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAAQCIPLVMEPESRFYSNSVIVLDFQSLYPSIVIAYNYCYSTCLGHIENLGTYDEFKFGCASLRVPPDLLYQLRNDITVSPNGVAFVKSSVRRGVLPRMLEEILKTRIMVKQSMKAYRNDKALSRLLDARQLGLKLIANVTFGYTSANFSGRMPCVEVGDSIVHKARETLERAIKMVNDT--KKWG----ARVVYGDTDSMFVLLKGATKEQAFKIGNEIAEAVTATNPKPIKLKFEKVYLPCILQTKKRYVGYMYESVDQENPVFDAKGIETVRRDGCPGVSKILERSLKLLFESRDISQVKQYVQRQCVKVVEGKASMQDLTFAKEYRGSGSYRPGACVPALELTRRMMAFDRRLEPRVGERVPYVIVYGSPGVPLIQLVRRPLEVLQDPSLRLNATYYITKQILPPLARIFNLIGVDVFSWYHELPRVQRAWSFTSSGRGEEGTKKGTISQYFTTL-HCPVCDELT---QLGVCARCRAEPQCVAVHLHQDLRLWESQQDQLLKICRSCSGSAERQVPCVSLDCPILYKLSRVNRNL 2764          
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: pola1 (polymerase (DNA directed), alpha 1 [Source:ZFIN;Acc:ZDB-GENE-030114-9])

HSP 1 Score: 193.356 bits (490), Expect = 2.103e-49
Identity = 219/852 (25.70%), Postives = 386/852 (45.31%), Query Frame = 3
Query:  765 LKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTEEWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNII-----VRFGESEPFIKNIFTL----NTCA-PIVGAHVIPHK--------TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRR--INKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQ-----------SKDGDE-------------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETD--PFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAG-MYYTKPDS--FDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQS---VIADLLCN-RIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDN-----NIGIDTDYYLKNQLTNPLLRIFEPIFGKE---LATRKLLHGEHVLSKFKQQSN 3131
            +K +  +PSL +  K     G T  H    N +S +        F+++  + G  W+++     + +P    IS+C++E   Q   +I    +  P   P+ ++S  ++        P    + ++ I+ ++     +      P  +  F +    N C  P      +  K        TE  LL  +   + +IDPD++ G++   FDL  L+ R N   V  +S +GR++         ++   ++G R    +     GR+  D+ +      + +SY+L  +    L+ ++  V  + I +  +      + L +  + D+ L+L++M +L V+   +++  + G  +S  L  G+  +    LL      DF++PD Q            ++GD              + G  V++P  GFYDK I  LDF SLYPSI+   N+C+TT+ +   + R+   TE+D  + P        S++ G+LP  + +L+E R++ K +L  E D  P      D RQ ALK++ANS+YG  G    R     +++ VT  GRE++  TKEMV+           + +VIYGDTDS+M     + + E   LG +    ++ K    ++++ + V+   LL+ KK+YA  M   + D     K + KG++ VRRD C L     N  + ++L  ++++  + + Q     I + + N  I ++   I K LTK  ++Y  K+  PH  +A  I  +  G   + GD V YVI     +D     A    Y L+       + +DT YYL  Q+   + RI EPI G +   +AT   L   H  ++ +Q+ +
Sbjct:  452 VKYSAEIPSLPSDLK-----GTTFSHVFGTNTSSLE-------HFLLNRKIRGPCWLDVTTPQLSNQP----ISWCKVEAVAQKSDLISVVKDLSP--PPLVVMSISLKTVQN----PKTHQNEIVSIAALVHHTFPLDKAPPRPPFQTHFCVVSKPNDCVFPYDFKDAVRKKNGIVEIAGTERALLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLR---------RANMPKLGGRSGFAEKSATCGRLVCDIEISAKELIRCKSYNLTELVSQILKAERPVVPPENIRNFYSDSTHLLYLLELTWM-DAKLILQIMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEYLLLHAFHERDFIVPDKQFFKKAQQDLGEDEEGDSGKGKSNKTRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVERGATNTRK--KTEEDEDEIP---ELPDQSLEMGILPKEIRKLVERRRQVK-QLMKEPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKEMVQR---------MNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVN-KLYKLLEIDIDGVFKSLLLLKKKKYAALMVEPQGDGKYTTKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQNRDTIIENIQKHLIEIGEKVANGNIPLNMFEIHKSLTKEPQDYPDKKSLPHVHVALWINSQG-GKKVKAGDTVSYVI----CQDGSNLAASQRAYTLEQLQKQPGLNLDTQYYLSQQVHPVVGRICEPIEGIDAVLIATWLGLDPSHFRAQQQQRED 1250          
BLAST of DNA polymerase vs. Ensembl Zebrafish
Match: pola1 (polymerase (DNA directed), alpha 1 [Source:ZFIN;Acc:ZDB-GENE-030114-9])

HSP 1 Score: 192.586 bits (488), Expect = 3.626e-49
Identity = 219/852 (25.70%), Postives = 386/852 (45.31%), Query Frame = 3
Query:  765 LKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTEEWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNII-----VRFGESEPFIKNIFTL----NTCA-PIVGAHVIPHK--------TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRR--INKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQ-----------SKDGDE-------------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETD--PFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAG-MYYTKPDS--FDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQS---VIADLLCN-RIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDN-----NIGIDTDYYLKNQLTNPLLRIFEPIFGKE---LATRKLLHGEHVLSKFKQQSN 3131
            +K +  +PSL +  K     G T  H    N +S +        F+++  + G  W+++     + +P    IS+C++E   Q   +I    +  P   P+ ++S  ++        P    + ++ I+ ++     +      P  +  F +    N C  P      +  K        TE  LL  +   + +IDPD++ G++   FDL  L+ R N   V  +S +GR++         ++   ++G R    +     GR+  D+ +      + +SY+L  +    L+ ++  V  + I +  +      + L +  + D+ L+L++M +L V+   +++  + G  +S  L  G+  +    LL      DF++PD Q            ++GD              + G  V++P  GFYDK I  LDF SLYPSI+   N+C+TT+ +   + R+   TE+D  + P        S++ G+LP  + +L+E R++ K +L  E D  P      D RQ ALK++ANS+YG  G    R     +++ VT  GRE++  TKEMV+           + +VIYGDTDS+M     + + E   LG +    ++ K    ++++ + V+   LL+ KK+YA  M   + D     K + KG++ VRRD C L     N  + ++L  ++++  + + Q     I + + N  I ++   I K LTK  ++Y  K+  PH  +A  I  +  G   + GD V YVI     +D     A    Y L+       + +DT YYL  Q+   + RI EPI G +   +AT   L   H  ++ +Q+ +
Sbjct:  464 VKYSAEIPSLPSDLK-----GTTFSHVFGTNTSSLE-------HFLLNRKIRGPCWLDVTTPQLSNQP----ISWCKVEAVAQKSDLISVVKDLSP--PPLVVMSISLKTVQN----PKTHQNEIVSIAALVHHTFPLDKAPPRPPFQTHFCVVSKPNDCVFPYDFKDAVRKKNGIVEIAGTERALLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLR---------RANMPKLGGRSGFAEKSATCGRLVCDIEISAKELIRCKSYNLTELVSQILKAERPVVPPENIRNFYSDSTHLLYLLELTWM-DAKLILQIMCELNVLPLALQITNIAGNVMSRTLMGGRSERNEYLLLHAFHERDFIVPDKQFFKKAQQDLGEDEEGDSGKGKSNKTRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVERGATNTRK--KTEEDEDEIP---ELPDQSLEMGILPKEIRKLVERRRQVK-QLMKEPDLNPDLYLQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKEMVQR---------MNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVN-KLYKLLEIDIDGVFKSLLLLKKKKYAALMVEPQGDGKYTTKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQNRDTIIENIQKHLIEIGEKVANGNIPLNMFEIHKSLTKEPQDYPDKKSLPHVHVALWINSQG-GKKVKAGDTVSYVI----CQDGSNLAASQRAYTLEQLQKQPGLNLDTQYYLSQQVHPVVGRICEPIEGIDAVLIATWLGLDPSHFRAQQQQRED 1262          
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744])

HSP 1 Score: 1155.58 bits (2988), Expect = 0.000e+0
Identity = 557/917 (60.74%), Postives = 701/917 (76.44%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE----------KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+       F+K+T+ +P L+A  KR+L +G+    +  H   +++ +IDF +RFMVD+ + GC+WIELP   Y +R +++          K+S  Q+EVD+ W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ D DPVIQI+N+++R GE + FI+N+FTL TC+ IVG+ V+    E  LL+ W++F+R IDPDIITGYN QNFD+PYLINRA  L V  F  LGRI+SM+S I +S  QSKQMGRR NK ++  GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRLM KLM V+NY+EMARVTGVPLSYLLSRGQQIKV+SQLLR+A  +D V+P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+S  SI ++GL+ +D+IKTP G  FVK+S++KGLLP IL  L+ ARK+AK EL  ETDPFK++VLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  DAKVIYGDTDSVMC  GV TVVEAM +G+EAA+ +S  F  PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  ++ DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD   A+ HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL  PLLRIFEPI G+  A   LL GEH   K    + VG L  +   +  CIGCK  L  D  ++C +CK  E  +  +E      LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q++++ R  
Sbjct:  181 NMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKYRLRKESQDEGPSKENPSKVSLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSG-SIDKYGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATLNHD-GAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRFG 1095          

HSP 2 Score: 66.2402 bits (160), Expect = 3.755e-10
Identity = 36/116 (31.03%), Postives = 55/116 (47.41%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNYEIKETN--------PRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ-TLKQRASQHQYAENLICKVEYCLK 541
            KW R  + +  P+   L FQQ+++D+Y     +        P  + R +G  + GNS+  H+HGF PY YV    GF +E   +F+K LN   +K   S        +  V+ C K
Sbjct:   64 KWLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHK 179          
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.000e+0
Identity = 557/917 (60.74%), Postives = 701/917 (76.44%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE----------KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+       F+K+T+ +P L+A  KR+L +G+    +  H   +++ +IDF +RFMVD+ + GC+WIELP   Y +R +++          K+S  Q+EVD+ W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ D DPVIQI+N+++R GE + FI+N+FTL TC+ IVG+ V+    E  LL+ W++F+R IDPDIITGYN QNFD+PYLINRA  L V  F  LGRI+SM+S I +S  QSKQMGRR NK ++  GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRLM KLM V+NY+EMARVTGVPLSYLLSRGQQIKV+SQLLR+A  +D V+P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+S  SI ++GL+ +D+IKTP G  FVK+S++KGLLP IL  L+ ARK+AK EL  ETDPFK++VLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  DAKVIYGDTDSVMC  GV TVVEAM +G+EAA+ +S  F  PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  ++ DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD   A+ HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL  PLLRIFEPI G+  A   LL GEH   K    + VG L  +   +  CIGCK  L  D  ++C +CK  E  +  +E      LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q++++ R  
Sbjct:  191 NMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKYRLRKESQDEGPSKENPSKVSLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSG-SIDKYGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATLNHD-GAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRFG 1105          

HSP 2 Score: 66.2402 bits (160), Expect = 3.448e-10
Identity = 51/190 (26.84%), Postives = 76/190 (40.00%), Query Frame = 2
Query:   53 FQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNHEPEEICFEMESNTETVKETDEFS------------------KWARQNMDVVKPEIMPLIFQQLDIDNYEIKETN--------PRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ-TLKQRASQHQYAENLICKVEYCLK 541
            F++ K  A     S+ +    D DD   D  +   EE+ F  E   E   E  E                    KW R  + +  P+   L FQQ+++D+Y     +        P  + R +G  + GNS+  H+HGF PY YV    GF +E   +F+K LN   +K   S        +  V+ C K
Sbjct:    3 FKRKKGQASGLPASQTKKLRGDWDD---DAPSQFEEELAFMDEVEAEMAMELSEGQLSADILPVGKLISDNIPPKWLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHK 189          
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744])

HSP 1 Score: 1121.69 bits (2900), Expect = 0.000e+0
Identity = 548/911 (60.15%), Postives = 686/911 (75.30%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIE----LPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+       F+K+T+ +P L+A  KR+L +G+    +  H   +++ +IDF +R+        C W      LP+    V       S  Q+EVD+ W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ D DPVIQI+N+++R GE + FI+N+FTL TC+ IVG+ V+    E  LL+ W++F+R IDPDIITGYN QNFD+PYLINRA  L V  F  LGRI+SM+S I +S  QSKQMGRR NK ++  GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRLM KLM V+NY+EMARVTGVPLSYLLSRGQQIKV+SQLLR+A  +D V+P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+S  SI ++GL+ +D+IKTP G  FVK+S++KGLLP IL  L+ ARK+AK EL  ETDPFK++VLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  DAKVIYGDTDSVMC  GV TVVEAM +G+EAA+ +S  F  PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  ++ DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD   A+ HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL  PLLRIFEPI G+  A   LL GEH   K    + VG L  +   +  CIGCK  L  D  ++C +CK  E  +  +E      LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q++++ R  
Sbjct:  191 NMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRYS-----HQCVWHHYRQYLPVGNRIV-------SLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSG-SIDKYGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATLNHD-GAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRFG 1087          

HSP 2 Score: 66.2402 bits (160), Expect = 3.500e-10
Identity = 51/190 (26.84%), Postives = 76/190 (40.00%), Query Frame = 2
Query:   53 FQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNHEPEEICFEMESNTETVKETDEFS------------------KWARQNMDVVKPEIMPLIFQQLDIDNYEIKETN--------PRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ-TLKQRASQHQYAENLICKVEYCLK 541
            F++ K  A     S+ +    D DD   D  +   EE+ F  E   E   E  E                    KW R  + +  P+   L FQQ+++D+Y     +        P  + R +G  + GNS+  H+HGF PY YV    GF +E   +F+K LN   +K   S        +  V+ C K
Sbjct:    3 FKRKKGQASGLPASQTKKLRGDWDD---DAPSQFEEELAFMDEVEAEMAMELSEGQLSADILPVGKLISDNIPPKWLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHK 189          
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744])

HSP 1 Score: 1108.21 bits (2865), Expect = 0.000e+0
Identity = 543/917 (59.21%), Postives = 682/917 (74.37%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE----------KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+       F+K+T+ +P L+A  KR+L +G+    +  H   +++ +IDF +RFMVD+ + GC+WIELP   Y +R +++          K+S  Q+EVD+ W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ D DPVIQI+N+++R GE + FI+N+FTL TC+ IVG+ V+    E  LL+ W++F+R IDPDIITGYN QNFD+PYLINRA  L V  F  LGRI+SM+S I +S  QSKQMGRR NK ++  GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRLM KLM V+NY+EMARVTGVPLSYLLSRGQQIKV+SQLLR+           Q    +E      +   + +YD PIATLDF+SLYPSIMMAHNLCYTTLL+S  SI ++GL+ +D+IKTP G  FVK+S++KGLLP IL  L+ ARK+AK EL  ETDPFK++VLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  DAKVIYGDTDSVMC  GV TVVEAM +G+EAA+ +S  F  PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  ++ DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD   A+ HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL  PLLRIFEPI G+  A   LL GEH   K    + VG L  +   +  CIGCK  L  D  ++C +CK  E  +  +E      LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q++++ R  
Sbjct:  191 NMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKYRLRKESQDEGPSKENPSKVSLAQIEVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQVR---------QHNPQNEQLSCYCVS-CREYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSG-SIDKYGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATLNHD-GAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRFG 1095          

HSP 2 Score: 66.6254 bits (161), Expect = 2.887e-10
Identity = 51/190 (26.84%), Postives = 76/190 (40.00%), Query Frame = 2
Query:   53 FQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNHEPEEICFEMESNTETVKETDEFS------------------KWARQNMDVVKPEIMPLIFQQLDIDNYEIKETN--------PRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ-TLKQRASQHQYAENLICKVEYCLK 541
            F++ K  A     S+ +    D DD   D  +   EE+ F  E   E   E  E                    KW R  + +  P+   L FQQ+++D+Y     +        P  + R +G  + GNS+  H+HGF PY YV    GF +E   +F+K LN   +K   S        +  V+ C K
Sbjct:    3 FKRKKGQASGLPASQTKKLRGDWDD---DAPSQFEEELAFMDEVEAEMAMELSEGQLSADILPVGKLISDNIPPKWLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHK 189          
BLAST of DNA polymerase vs. Ensembl Xenopus
Match: plscr2 (phospholipid scramblase 2 [Source:Xenbase;Acc:XB-GENE-5819744])

HSP 1 Score: 1095.88 bits (2833), Expect = 0.000e+0
Identity = 539/907 (59.43%), Postives = 674/907 (74.31%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+       F+K+T+ +P L+A  KR+L +G+    +  H   +++ +IDF +RFMVD+ + GC+WIELP   Y            +L+VD+ W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ D DPVIQI+N+++R GE + FI+N+FTL TC+ IVG+ V+    E  LL+ W++F+R IDPDIITGYN QNFD+PYLINRA  L V  F  LGRI+SM+S I +S  QSKQMGRR NK ++  GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRLM KLM V+NY+EMARVTGVPLSYLLSRGQQIKV+SQLLR+           Q    +E      +   + +YD PIATLDF+SLYPSIMMAHNLCYTTLL+S  SI ++GL+ +D+IKTP G  FVK+S++KGLLP IL  L+ ARK+AK EL  ETDPFK++VLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  DAKVIYGDTDSVMC  GV TVVEAM +G+EAA+ +S  F  PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  ++ DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD   A+ HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL  PLLRIFEPI G+  A   LL GEH   K    + VG L  +   +  CIGCK  L  D  ++C +CK  E  +  +E      LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q++++ R  
Sbjct:  194 NMYGYHGKKIIPFMKITMALPRLIAPAKRLLEQGLRFGRHPTHCYQAYEANIDFEIRFMVDNDIVGCNWIELPAGKY------------RLQVDISWADLISHPAEGDWQKIAPLRVLSFDIECAGRKGVFPEPDKDPVIQIANMVLRQGEKDAFIRNVFTLGTCSSIVGSQVLCFDREDALLKAWADFVRTIDPDIITGYNIQNFDMPYLINRAQTLKVPNFPFLGRIRSMKSVIRDSSFQSKQMGRRENKVINTEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLMEKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQVR---------QHNPQNEQLSCYCVS-CREYYDVPIATLDFSSLYPSIMMAHNLCYTTLLQSG-SIDKYGLSPEDFIKTPTGDCFVKASIRKGLLPEILENLLCARKRAKAELKKETDPFKQKVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLDNGYKADAKVIYGDTDSVMCKLGVQTVVEAMEIGREAAEWVSSHFTPPIKLEFEKVYFPYLLINKKRYAGLYFSSSANTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPMGAVEHAKDVISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIIGAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGESKAESILLKGEHTRCKTVLTAKVGGLMAFAKKRSTCIGCKATLNHD-GAVCNYCKQRESELIQKEISQLSGLEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQEKLILRFG 1076          

HSP 2 Score: 65.855 bits (159), Expect = 4.276e-10
Identity = 36/116 (31.03%), Postives = 55/116 (47.41%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNYEIKETN--------PRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ-TLKQRASQHQYAENLICKVEYCLK 541
            KW R  + +  P+   L FQQ+++D+Y     +        P  + R +G  + GNS+  H+HGF PY YV    GF +E   +F+K LN   +K   S        +  V+ C K
Sbjct:   77 KWLRPPVSLTDPKEQTLCFQQVELDHYVGSHVSGMLGATQGPVPIIRMFGITEEGNSVCCHIHGFAPYFYVPCHTGFKQEDLSDFKKELNSAVIKDMRSNKDSISQAVLAVDVCHK 192          
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:MGI Symbol;Acc:MGI:97741])

HSP 1 Score: 1121.3 bits (2899), Expect = 0.000e+0
Identity = 527/906 (58.17%), Postives = 695/906 (76.71%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++T+ +P L+A  +R+L +G+ +P  G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y VR   +K + CQLEVDV W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ W++FI  +DPD+ITGYN QNFDLPYLI+RA  L V  F  LGR+  +RS I +S  QS+Q+GRR +K + M GR+  D+  +LLR+HKLRSY+LNAVSFHFL EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPL YLL+RGQQ+KV+SQLLR+A  +  ++P  +++  +++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GL  D++IKTP G  FVKSSV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  +AKV+YGDTDSVMC FGVS+V EAM+LG+EAA  +S  F +PI+LEFEKVY+PYLLISKKRYAG+ + ++ D+ DKMDCKG+E VRRDNCPLVANL+ + LR++L+ RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K    S VG L  +T  +  CIGC++V+ D + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q+ +++R  
Sbjct:  196 MFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKY-VRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-AAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVI-DHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFG 1098          

HSP 2 Score: 73.559 bits (179), Expect = 1.351e-12
Identity = 38/121 (31.40%), Postives = 57/121 (47.11%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLK--QRASQHQYAENLICKVEYCLKAS 547
            +W R  +  + P   PLIFQQL+ID+Y           +   N   + R +G    G S+  H+ GF PY Y   PPGF  EH  E ++ LN  +   QR  + + +   +  +E C + S
Sbjct:   76 RWRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGK-ELSGPAVLAIELCSRES 195          
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:MGI Symbol;Acc:MGI:97741])

HSP 1 Score: 910.212 bits (2351), Expect = 0.000e+0
Identity = 428/711 (60.20%), Postives = 559/711 (78.62%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQK 2855
            ++G+  +    FL++T+ +P L+A  +R+L +G+ +P  G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y VR   +K + CQLEVDV W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ W++FI  +DPD+ITGYN QNFDLPYLI+RA  L V  F  LGR+  +RS I +S  QS+Q+GRR +K + M GR+  D+  +LLR+HKLRSY+LNAVSFHFL EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPL YLL+RGQQ+KV+SQLLR+A  +  ++P  +++  +++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GL  D++IKTP G  FVKSSV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  +AKV+YGDTDSVMC FGVS+V EAM+LG+EAA  +S  F +PI+LEFEKVY+PYLLISKKRYAG+ + ++ D+ DKMDCKG+E VRRDNCPLVANL+ + LR++L+ RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++
Sbjct:  196 MFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKY-VRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-AAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAER 904          

HSP 2 Score: 73.9442 bits (180), Expect = 1.013e-12
Identity = 38/121 (31.40%), Postives = 57/121 (47.11%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLK--QRASQHQYAENLICKVEYCLKAS 547
            +W R  +  + P   PLIFQQL+ID+Y           +   N   + R +G    G S+  H+ GF PY Y   PPGF  EH  E ++ LN  +   QR  + + +   +  +E C + S
Sbjct:   76 RWRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGK-ELSGPAVLAIELCSRES 195          
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:MGI Symbol;Acc:MGI:1337131])

HSP 1 Score: 302.753 bits (774), Expect = 9.198e-84
Identity = 228/777 (29.34%), Postives = 384/777 (49.42%), Query Frame = 3
Query: 1224 IVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITES-QSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITD-LQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDF--VLPDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIRE-----FGLTE------------DDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKREL-AIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFEPI------FGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCK--PNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQ 3443
            I G  V     E  L +  +N I+  DPDI+ GY  Q     YL+ RA  L+V    ++ R+     KI     ++    G      +++ GRI  +L+ I+  +  L +Y+   VSFH L ++      ++++D   N  D  R ++  + +      L+++ +L ++    EMAR+ G+   ++L+RG Q +V S +LR A   ++  V P  Q +           ++EP   FY   +  LDF SLYPSI++A+N C++T L   E++ +     FG T              D   +P G  FVK SV+KG+LP +L ++++ R   K+ + + + D    ++L+ RQL LK+ AN  +G+T A   GR+PC+ +  S+    RE ++   ++V  +  K  G    A+V+YGDTDS+  L   +T  ++  +G+E A++++     P+KL+FEKVY P +L +KKRY G  Y   D  + + D KG+ETVRRD+CP V+ +L   L+ L  +RD +    + Q     L+  +  I   I +KE  +    Y      P  EL +K+   D  S P++G+RVPYVI        +      P  VL D  + ++  YY+  Q+  PL RIF  I      + +EL     +      S+ + +   G +S+Y      C  C ++    +  IC+ C+  P  +AI   +    + LE +  +L   C+ C+GS  + + C + +CP+ +   +V +EL     + + L+Q
Sbjct: 2363 ITGLEVTYAADEKALFQEITNIIKRYDPDILLGYEIQMHSWGYLLQRAAALSVDLCQMISRVPD--DKIENRFAAERDDYGSDTMSEINIVGRITLNLWRIMRNEVALTNYTFENVSFHVLHQRFPLFTFRVLSDWFDNKTDLYRWKMVDHYVSRVRGNLQMLEQLDLIGKTSEMARLFGIQFLHVLTRGSQYRVESMMLRIAKPMNYIPVTPSIQQRSQMRAPQCVPLIMEPESRFYSNSVLVLDFQSLYPSIVIAYNYCFSTCLGHVENLGKYDEFKFGCTSLRVPPDLLYQIRHDVTVSPNGVAFVKPSVRKGVLPRMLEEILKTRLMVKQSMKSYKQDRALSRMLNARQLGLKLIANVTFGYTAANFSGRMPCIEVGDSIVHKARETLERAIKLVNDT--KKWG----ARVVYGDTDSMFVLLKGATKEQSFKIGQEIAEAVTATNPRPVKLKFEKVYLPCVLQTKKRYVGYMYETLDQKEPVFDAKGIETVRRDSCPAVSKILERSLKLLFETRDISLIKQYVQRQCMKLVEGKASIQDFIFAKEY-RGSFSYRPGACVPALELTRKMLAYDRRSEPRVGERVPYVIIYGTPGLPLIQLIRRPAEVLQDPTLRLNATYYITKQILPPLARIFSLIGIDVFSWYQELPR---IQKATSSSRSELEGRKGTISQYFTTL-HCPVCDDLT---QHGICSKCRSQPQHVAIILNQEI--RELERKQEQLIKICRNCTGSFDRHIPCVSLNCPVLFKLSRVNRELSKAPYLRQLLDQ 3121          
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:MGI Symbol;Acc:MGI:1337131])

HSP 1 Score: 302.753 bits (774), Expect = 9.198e-84
Identity = 228/777 (29.34%), Postives = 384/777 (49.42%), Query Frame = 3
Query: 1224 IVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITES-QSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITD-LQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDF--VLPDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIRE-----FGLTE------------DDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKREL-AIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFEPI------FGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCK--PNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQ 3443
            I G  V     E  L +  +N I+  DPDI+ GY  Q     YL+ RA  L+V    ++ R+     KI     ++    G      +++ GRI  +L+ I+  +  L +Y+   VSFH L ++      ++++D   N  D  R ++  + +      L+++ +L ++    EMAR+ G+   ++L+RG Q +V S +LR A   ++  V P  Q +           ++EP   FY   +  LDF SLYPSI++A+N C++T L   E++ +     FG T              D   +P G  FVK SV+KG+LP +L ++++ R   K+ + + + D    ++L+ RQL LK+ AN  +G+T A   GR+PC+ +  S+    RE ++   ++V  +  K  G    A+V+YGDTDS+  L   +T  ++  +G+E A++++     P+KL+FEKVY P +L +KKRY G  Y   D  + + D KG+ETVRRD+CP V+ +L   L+ L  +RD +    + Q     L+  +  I   I +KE  +    Y      P  EL +K+   D  S P++G+RVPYVI        +      P  VL D  + ++  YY+  Q+  PL RIF  I      + +EL     +      S+ + +   G +S+Y      C  C ++    +  IC+ C+  P  +AI   +    + LE +  +L   C+ C+GS  + + C + +CP+ +   +V +EL     + + L+Q
Sbjct: 2363 ITGLEVTYAADEKALFQEITNIIKRYDPDILLGYEIQMHSWGYLLQRAAALSVDLCQMISRVPD--DKIENRFAAERDDYGSDTMSEINIVGRITLNLWRIMRNEVALTNYTFENVSFHVLHQRFPLFTFRVLSDWFDNKTDLYRWKMVDHYVSRVRGNLQMLEQLDLIGKTSEMARLFGIQFLHVLTRGSQYRVESMMLRIAKPMNYIPVTPSIQQRSQMRAPQCVPLIMEPESRFYSNSVLVLDFQSLYPSIVIAYNYCFSTCLGHVENLGKYDEFKFGCTSLRVPPDLLYQIRHDVTVSPNGVAFVKPSVRKGVLPRMLEEILKTRLMVKQSMKSYKQDRALSRMLNARQLGLKLIANVTFGYTAANFSGRMPCIEVGDSIVHKARETLERAIKLVNDT--KKWG----ARVVYGDTDSMFVLLKGATKEQSFKIGQEIAEAVTATNPRPVKLKFEKVYLPCVLQTKKRYVGYMYETLDQKEPVFDAKGIETVRRDSCPAVSKILERSLKLLFETRDISLIKQYVQRQCMKLVEGKASIQDFIFAKEY-RGSFSYRPGACVPALELTRKMLAYDRRSEPRVGERVPYVIIYGTPGLPLIQLIRRPAEVLQDPTLRLNATYYITKQILPPLARIFSLIGIDVFSWYQELPR---IQKATSSSRSELEGRKGTISQYFTTL-HCPVCDDLT---QHGICSKCRSQPQHVAIILNQEI--RELERKQEQLIKICRNCTGSFDRHIPCVSLNCPVLFKLSRVNRELSKAPYLRQLLDQ 3121          
BLAST of DNA polymerase vs. Ensembl Mouse
Match: Pola1 (polymerase (DNA directed), alpha 1 [Source:MGI Symbol;Acc:MGI:99660])

HSP 1 Score: 193.741 bits (491), Expect = 1.621e-49
Identity = 186/642 (28.97%), Postives = 317/642 (49.38%), Query Frame = 3
Query: 1254 TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQ------SKDGDE-------------------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVL--DGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDS----FDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQ----SVIADLLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDP--IYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFG 3062
            TE  L+  +   + +IDPDI+ G+N  +F+L  L+ R N+  V  +S +GR++  RS + +  S+S   G R N      GR+  D+ +        +SY L+ +    L+ ++  +  + I ++ +      + L  +  KD+  +L++M +L V+   +++  + G  +S  L  G+  +    LL      ++++PD Q       K GDE                   + G  V++P  GFYDK I  LDF SLYPSI+   N+C+TT+ +    +++   TED+  +          +++ G+LP  + +L+E RK+ K +L  + D     VL  D RQ ALK++ANS+YG  G    R     +++ VT  GRE++  TK+MV+           + +VIYGDTDS+M     + + E   LG +    ++ K    ++++ + V+   LL+ KK+YA +   +P S      K + KG++ VRRD C L  +  N  + ++L  + ++  + + Q     +  ++L   + +SQ  I+K LTK  ++Y  ++  PH  +A  I  +  G   + GD V YVI   G       +A  P  +  LD N+ IDT YYL  Q+   + RI EPI G
Sbjct:  618 TERTLIGFFLAKVHKIDPDILVGHNICSFELEVLLQRINECKVPYWSKIGRLR--RSNMPKLGSRSG-FGER-NATC---GRMICDVEISAKELIHCKSYHLSELVQQILKTERIVIPTENIRNMYSESSYLLYLLE-HIWKDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVTSEVQK--ATEDE--EQEQIPELPDPNLEMGILPREIRKLVERRKQVK-QLMKQQDLNPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQK---------MNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVN-KLYKLLEIDIDAVFKSLLLLKKKKYAAL-VVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQG-GRKVKAGDTVSYVICQDGSNLTATQRAYAPEQLQKLD-NLAIDTQYYLAQQIHPVVARICEPIDG 1233          
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P28339|DPOD1_BOVIN (DNA polymerase delta catalytic subunit OS=Bos taurus OX=9913 GN=POLD1 PE=1 SV=1)

HSP 1 Score: 1140.18 bits (2948), Expect = 0.000e+0
Identity = 537/906 (59.27%), Postives = 693/906 (76.49%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++T+ +P L+A  +R+L +G+ +   G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y +RP+  K + CQLE DV W  +I H  E EW  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ WS FIR +DPD+ITGYN QNFDLPYLI+RA  L V GF LLGR+  +RS I ES  QS+Q GRR +K V M GR+  D+  +LLR++KLRSY+LNAVSFHFL EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL+ +LMV+VN +EMARVTGVPL YLLSRGQQ+KV+SQLLR+A  +  ++P  +++ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GLTED +IKTP G  FVK+SV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ GRLPCL IS SVT FGR+MI+ TK++VE+ +   NGY   AKV+YGDTDSVMC FGVS+V EAMALG+EAA  +SG F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+   LR+LLI RD + A++HAQ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI +       Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K      VG L  +   +  CIGC+ VL   + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q+ ++ R  
Sbjct:  197 MFGYHGHGPSPFLRITLALPRLMAPARRLLEQGIRLAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYILRPEG-KATLCQLEADVLWSDVISHPPEGEWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVPGFPLLGRVIGLRSNIRESSFQSRQTGRRDSKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAFLPLRLLERLMVLVNAMEMARVTGVPLGYLLSRGQQVKVVSQLLRQAMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAA-QKLGLTEDQFIKTPTGDEFVKASVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGRLPCLEISQSVTGFGRQMIEKTKQLVETKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPSGAVAHAQDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCKFCQPRESELYQKEVSHLSALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLRRFG 1099          

HSP 2 Score: 73.559 bits (179), Expect = 9.779e-12
Identity = 40/122 (32.79%), Postives = 60/122 (49.18%), Query Frame = 2
Query:  218 SKWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLK--QRASQHQYAENLICKVEYCLKAS 547
            ++W R     + P++ PLIFQQL+ID+Y      +    P S     + R +G    G S+  H+HGF PY Y   PPGF  EH  E ++ L+  +   QR  +      ++  VE C + S
Sbjct:   76 ARWLRPAPPALDPQMEPLIFQQLEIDHYVAPARPLPGAPPPSQDSVPILRAFGVTNEGVSVCCHIHGFAPYFYTPAPPGFGPEHLSELQRELSAAISRDQRGGKELTGPAVLA-VELCSRES 196          
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P54358|DPOD1_DROME (DNA polymerase delta catalytic subunit OS=Drosophila melanogaster OX=7227 GN=DNApol-delta PE=1 SV=2)

HSP 1 Score: 1135.94 bits (2937), Expect = 0.000e+0
Identity = 545/915 (59.56%), Postives = 700/915 (76.50%), Query Frame = 3
Query:  723 LDIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKI---SFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDE-FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLL--KSKESIREFGLTEDDYI-KTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRD--DEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERL 3437
            L+I+G++ + K+ ++K++V +P  VA   R+L + + +    + +  +F+ +IDF +RFMVD+ + GC+WIELP+  + +R    K    S CQ+EVDV +D+ I H  E EW  +AP RILSFDIECAGRKG FP+A IDPVIQI+N+++R GE EPFI+N+FTLN CAPI+G+ V+ H  E ++L+ WS F+RE+DPDI+TGYN  NFD PYL+NRA  L V+ F  LGRIK++RS I E   QSKQMGRR N+ V+  GR+ FDL  +LLRD+KLRSY+LNAVS+HFL+EQKEDV H IITDLQNGD++TR RLA+YCLKD+ L LRL+ KLM +VNY+EMARVTGVPL  LL+RGQQIKVLSQLLRKA  + F++P Y S+  DE + GATVIEP +G+Y  PI+TLDFASLYPSIMMAHNLCYTTL+   ++E +R+    +DD + +TP  +YFVKS V++GLLP IL  L+ ARK+AK +L +ETDPFKR+VLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVTA+GR MI+ TK  VES + +ANGY ++A VIYGDTDSVM  FGV T+  +M LG+EAA+ +S KF+ PIKLEFEKVYYPYLLI+KKRYAG+Y+T+PD++DKMDCKG+ETVRRDN PLVANL+N+CL+KLLI RD + A+++ + VIADLLCNRIDIS L+I+KEL KTD  Y +KQ H ELA K+KKRDPG+AP+LGDRVPYVI         Y KAEDP+YVL+N++ ID  YYL+ QL+ PLLRIFEPI G   A   LL GEH  ++    S VG L+ +   K  C+GCK+++    ++  +C HC+P    +Y +E    + LE  FSRLWTECQRC  SLH+EV+CSNRDCPIFY R+KV+ +L +Q++ + R 
Sbjct:  176 LNIHGYNGDKKQRYIKISVTLPRFVAAASRLLKKEVIMSEIDFQDCRAFENNIDFDIRFMVDTDVVGCNWIELPMGHWRIRNSHSKPLPESRCQIEVDVAFDRFISHEPEGEWSKVAPFRILSFDIECAGRKGIFPEAKIDPVIQIANMVIRQGEREPFIRNVFTLNECAPIIGSQVLCHDKETQMLDKWSAFVREVDPDILTGYNINNFDFPYLLNRAAHLKVRNFEYLGRIKNIRSVIKEQMLQSKQMGRRENQYVNFEGRVPFDLLFVLLRDYKLRSYTLNAVSYHFLQEQKEDVHHSIITDLQNGDEQTRRRLAMYCLKDAYLPLRLLEKLMAIVNYMEMARVTGVPLESLLTRGQQIKVLSQLLRKAKTKGFIMPSYTSQGSDEQYEGATVIEPKRGYYADPISTLDFASLYPSIMMAHNLCYTTLVLGGTREKLRQQENLQDDQVERTPANNYFVKSEVRRGLLPEILESLLAARKRAKNDLKVETDPFKRKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISGSVTAYGRTMIEMTKNEVESHYTQANGYENNAVVIYGDTDSVMVNFGVKTLERSMELGREAAELVSSKFVHPIKLEFEKVYYPYLLINKKRYAGLYFTRPDTYDKMDCKGIETVRRDNSPLVANLMNSCLQKLLIERDPDGAVAYVKQVIADLLCNRIDISHLVITKELAKTD--YAAKQAHVELAAKMKKRDPGTAPKLGDRVPYVICAAAKNTPAYQKAEDPLYVLENSVPIDATYYLEQQLSKPLLRIFEPILGDN-AESILLKGEHTRTRTVVTSKVGGLAGFMTKKTSCLGCKSLMPKGYEQACLCPHCEPRMSELYQKEVGAKRELEETFSRLWTECQRCQESLHEEVICSNRDCPIFYMRQKVRMDLDNQEKRVLRF 1087          

HSP 2 Score: 69.3218 bits (168), Expect = 2.116e-10
Identity = 34/91 (37.36%), Postives = 48/91 (52.75%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNYEIK--------ETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ 469
            +W+R     + P    L FQQLD++NY  +        +  P  V R +G    GNS+  HVHGF PY Y+E P  F + HC + +K L+Q
Sbjct:   60 RWSRPPPPELDPSKHNLEFQQLDVENYLGQPLPGMPGAQIGPVPVVRMFGVTMEGNSVCCHVHGFCPYFYIEAPSQFEEHHCEKLQKALDQ 150          
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P28340|DPOD1_HUMAN (DNA polymerase delta catalytic subunit OS=Homo sapiens OX=9606 GN=POLD1 PE=1 SV=2)

HSP 1 Score: 1131.32 bits (2925), Expect = 0.000e+0
Identity = 537/906 (59.27%), Postives = 690/906 (76.16%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++TV +P LVA  +R+L +G+ +   G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y +R + EK + CQLE DV W  ++ H  E  W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ WS FIR +DPD+ITGYN QNFDLPYLI+RA  L V+ F  LGR+  + S I +S  QSKQ GRR  K V M GR+  D+  +LLR++KLRSY+LNAVSFHFL EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPLSYLLSRGQQ+KV+SQLLR+A  E  ++P  +S+ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GLTED +I+TP G  FVK+SV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY   AKV+YGDTDSVMC FGVS+V EAMALG+EAA  +SG F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+   LR+LLI RD   A++HAQ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI +       Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K      VG L  +   +  CIGC+ VL   + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q++++ R  
Sbjct:  198 MFGYHGHGPSPFLRITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLK-EKATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPFLGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLLRQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-TAQKLGLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAEAMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVL-SHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFG 1100          

HSP 2 Score: 75.485 bits (184), Expect = 2.582e-12
Identity = 40/120 (33.33%), Postives = 58/120 (48.33%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRA-SQHQYAENLICKVEYCLKAS 547
            +W R     + P+  PLIFQQL+ID+Y      +    P S     V R +G    G S+  H+HGF PY Y   PPGF  EH G+ ++ LN  + + +    +     +  VE C + S
Sbjct:   78 RWLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSRGSVPVLRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRES 197          
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P97283|DPOD1_MESAU (DNA polymerase delta catalytic subunit OS=Mesocricetus auratus OX=10036 GN=POLD1 PE=2 SV=1)

HSP 1 Score: 1122.07 bits (2901), Expect = 0.000e+0
Identity = 528/906 (58.28%), Postives = 695/906 (76.71%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++T+ +P LVA  +R+L +G+ +P  G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y  R + +K + CQLEVDV W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ W NFI  +DPD+ITGYN QNFDLPYLI+RA  L V  F  LGR+  +RS I +S  QS+Q+GRR +K V M GR+  D+  +LLR+HKLRSY+LNAVSFHFL EQKEDV H IITDLQNG+++TR RLAVYCL+D+ L LRL+ +LMV+VN VEMARVTGVPL YLLSRGQQ+KV+SQLLR+A  +  ++P  +++ G+++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GL  D++IKTP G  FVKSSV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGA+ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  +AKV+YGDTDSVMC FGVS+V EAM+LG+EAA  +S  F +PI+LEFEKVY+PYLLISKKRYAG+ + ++PD+ D+MDCKG+E VRRDNCPLVANL+ + LR++L+ RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDP++VL++++ IDT YYL  QL  PLLRIFEPI G+  A   LL G+H   K    S VG L  +T  +  CIGC++V+ + + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q+ +++R  
Sbjct:  194 MFGYHGHGPSPFLRITLALPRLVAPARRLLEQGIRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVWRTE-KKATQCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWPNFILAMDPDVITGYNIQNFDLPYLISRAQTLKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVVSMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLRDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLSRGQQVKVVSQLLRQAMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-AAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAEVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTLENGYNANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSQPDTHDRMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLGQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVI-NHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFG 1096          

HSP 2 Score: 71.2478 bits (173), Expect = 5.752e-11
Identity = 38/121 (31.40%), Postives = 59/121 (48.76%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY-----EIKETNPRS-----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLK--QRASQHQYAENLICKVEYCLKAS 547
            +W R  +  + P   PLIFQQL+ID+Y      +    P S     + R +G    G S+  H+HGF PY Y   PPGF  EH  + ++ L+  +   QR  + + +   +  +E C + S
Sbjct:   74 RWKRPALCALDPNTEPLIFQQLEIDHYVGSAVPLPGGPPTSCNSVPILRAFGVTDEGFSVCCHIHGFAPYFYTPAPPGFGAEHLSDLQRELSTAISRDQRGGK-ELSGPAVLAIELCSRES 193          
BLAST of DNA polymerase vs. UniProt/SwissProt
Match: sp|P52431|DPOD1_MOUSE (DNA polymerase delta catalytic subunit OS=Mus musculus OX=10090 GN=Pold1 PE=1 SV=2)

HSP 1 Score: 1121.3 bits (2899), Expect = 0.000e+0
Identity = 527/906 (58.17%), Postives = 695/906 (76.71%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++G+  +    FL++T+ +P L+A  +R+L +G+ +P  G  + A ++ ++DF +RFMVD+ + GC+W+ELP   Y VR   +K + CQLEVDV W  +I H  E +W  IAP+R+LSFDIECAGRKG FP+ + DPVIQI ++ +R+GE EPF++   TL  CAPI+GA V  ++ E +LL+ W++FI  +DPD+ITGYN QNFDLPYLI+RA  L V  F  LGR+  +RS I +S  QS+Q+GRR +K + M GR+  D+  +LLR+HKLRSY+LNAVSFHFL EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ +LMV+VN VEMARVTGVPL YLL+RGQQ+KV+SQLLR+A  +  ++P  +++  +++TGATVIEP+KG+YD PIATLDF+SLYPSIMMAHNLCYTTLL+   + ++ GL  D++IKTP G  FVKSSV+KGLLP IL  L+ ARK+AK ELA ETDP +RQVLDGRQLALK+SANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +   NGY  +AKV+YGDTDSVMC FGVS+V EAM+LG+EAA  +S  F +PI+LEFEKVY+PYLLISKKRYAG+ + ++ D+ DKMDCKG+E VRRDNCPLVANL+ + LR++L+ RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+   +Y  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDP++VL++++ IDT YYL+ QL  PLLRIFEPI G+  A   LL G+H   K    S VG L  +T  +  CIGC++V+ D + ++C  C+P E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++V+C++RDCPIFY RKKV+K+L  Q+ +++R  
Sbjct:  196 MFGYHGHGPSPFLRITLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKY-VRRAEKKATLCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLRLALTLRPCAPILGAKVQSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLGRVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQAMRQGLLMPVVKTEGSEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPG-AAQKLGLKPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYDANAKVVYGDTDSVMCRFGVSSVAEAMSLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVANLVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESVLLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVI-DHQGAVCKFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFG 1098          

HSP 2 Score: 73.559 bits (179), Expect = 9.449e-12
Identity = 38/121 (31.40%), Postives = 57/121 (47.11%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLK--QRASQHQYAENLICKVEYCLKAS 547
            +W R  +  + P   PLIFQQL+ID+Y           +   N   + R +G    G S+  H+ GF PY Y   PPGF  EH  E ++ LN  +   QR  + + +   +  +E C + S
Sbjct:   76 RWRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPLPSRNSVPILRAFGVTDEGFSVCCHIQGFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGK-ELSGPAVLAIELCSRES 195          
BLAST of DNA polymerase vs. TrEMBL
Match: K1PLY1 (DNA polymerase OS=Crassostrea gigas OX=29159 GN=CGI_10007383 PE=3 SV=1)

HSP 1 Score: 1216.45 bits (3146), Expect = 0.000e+0
Identity = 583/911 (64.00%), Postives = 714/911 (78.38%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVR--FMVDSGLDGCSWIELPINTYTVR----PQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            IYG+  N K  FLK+TV +P L+A  KR+L +G T P Y  +   +++++IDF VR  FMVD+ + GCSWIE+P   Y +R    P+    S CQ+EVDV W+  I H  E EW ++AP RILS+DIECAGRKG FP+ D DPVIQI+N+++R GE +PFI+N+FTL +CAP+VG+ VI ++ E++LL+ W+ F+R +DPDIITGYN QNFD PY+INRAN L V  F+ LGR + +RS I ES  QSKQMG+R NK +++ GR+ FDL  ILLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+D+TR RLAVYCLKD+ L LRL++KLM V+NY+EMARVTGVPL YLLSRGQQ+KV+SQLLRKA  +D VLP ++ + GDE+ GATVIEP+KGFYD PIATLDF+SLYPSIMMAHNLCYTTLL S+ SI++ GLT D YIKTP G+YF+K+  +KGLLP IL  L+ ARKKAK +L  ETDPFK++VLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVTAFGR MI+ TK  VE  +  ANGY HDAKVIYGDTDSVMC FGV TV EAM LG+EAA  IS KFI PIKLEFEKVY+PYLLI+KKRYAG+Y+T P+  DKMDCKG+ETVRRDN PLVANL+NTCL+ +LI+RD   A+ +A+  I+DLLCNRIDISQL+I+KELTKT +EY  KQ H ELA ++KKRDPGSAPQLGDRVPYVI         Y  +EDPIYVL+NNI IDT YYL+NQL+ PLLRIFEPI G++ A  +LL G+H  +K K  S VG L  +   K  CIGC+ VL++D  ++C +CKP E  IY +E  +   LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  QD+++ R  
Sbjct:  177 IYGYHGNKKIPFLKITVAIPRLIAPAKRLLEQGFTCPGYSSNGFQAYESNIDFEVRQEFMVDTDVVGCSWIEIPAGKYRIRTKDDPKFPVKSRCQMEVDVSWEDFISHVPEGEWSAMAPFRILSYDIECAGRKGVFPEPDKDPVIQIANMVIRQGERDPFIRNVFTLRSCAPVVGSQVISNEKESDLLKKWAEFVRVVDPDIITGYNIQNFDFPYVINRANHLKVMDFTFLGRQRDVRSVIKESMFQSKQMGKRENKVINIEGRVQFDLLQILLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNDQTRRRLAVYCLKDAILPLRLLDKLMCVINYMEMARVTGVPLPYLLSRGQQVKVVSQLLRKAKEQDLVLPTHKVETGDEYEGATVIEPVKGFYDSPIATLDFSSLYPSIMMAHNLCYTTLL-SQHSIQKEGLTPDQYIKTPSGNYFIKTQHRKGLLPEILEHLLSARKKAKADLKKETDPFKKKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTAFGRMMIEQTKTYVEEHYTIANGYQHDAKVIYGDTDSVMCKFGVGTVAEAMKLGQEAADLISEKFIKPIKLEFEKVYFPYLLINKKRYAGLYWTNPEKHDKMDCKGIETVRRDNSPLVANLINTCLQLILINRDPQGAVEYAKQAISDLLCNRIDISQLVITKELTKTGDEYAGKQAHVELAHRMKKRDPGSAPQLGDRVPYVIIAGTKGSAAYMNSEDPIYVLENNIAIDTQYYLENQLSKPLLRIFEPILGEKKAESELLRGDHTRTKTKVASKVGGLMAFAKKKATCIGCRTVLQEDGIAVCKYCKPKESEIYQKEISHLNILEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLADQDKLLHRFG 1086          

HSP 2 Score: 86.2705 bits (212), Expect = 3.528e-13
Identity = 58/179 (32.40%), Postives = 89/179 (49.72%), Query Frame = 2
Query:   65 KTYAPSSKISKFEVKNNDEDDVS-MDEWNHEPEEICFEMESNTETVKETDEFS--------KWARQNMDVVKPEIMPLIFQQLDIDNYEIKET--------NPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLK-QRASQHQYAENLICKVEYCLKAS 547
            K   P +K  +F    NDED+ S  ++     +EI  EM+ N +    T  +S        KW R  +  +K +   LIFQQ+D+D+Y  +           P  V R YG   NGNS++AH+HGF PY +V    GF +EHC +FRK L+  ++    S  +  +  +  V++ +K S
Sbjct:    2 KKGGPPNKKPRF----NDEDEPSHFEDELALLDEIEMEMQDNFDEPDGTAGYSPEDVNAPTKWCRPPLPKMKADKDKLIFQQIDVDHYIGRHLPGMPGATQGPVPVIRMYGVTLNGNSVLAHIHGFAPYFFVPAQQGFKREHCEQFRKHLDSVVRGDLRSNREEIQTAVLAVDFVMKES 176          
BLAST of DNA polymerase vs. TrEMBL
Match: R7TGI4 (DNA polymerase OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_165849 PE=3 SV=1)

HSP 1 Score: 1216.45 bits (3146), Expect = 0.000e+0
Identity = 570/905 (62.98%), Postives = 708/905 (78.23%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            IYG+  N K  +LK+T+ +P L+A  KR+L +G     Y  HN A ++++IDF +RFMVD+ + GC+WI LP   Y VRP+    + CQ+E DV WD+ + H  E +W  +AP+  LSFDIECAGRKG FP+ D DPVIQI+N+++R GE EPFI+N+FTL  CAPIVGA V+  ++EAELL+ W+ FIRE+DPD+ITGYN QNFDLPYL+NRA  L VK F  LGRIK ++S + E+  QSKQMG+R NK ++M GR+ FDL  +LLRD+KLRSY+LNAVSFHFLEEQKEDV H II+DLQNG D+TR RLAVYCLKD+ L LRL++KLM ++NY+EMARVTGV L  LLSRGQQIKV+SQLLRKA  +D V+P Y+ + GDEFTGATVIEP++G+YD P+ATLDFASLYPSIMMAHNLCYTTL+ +  SI + GL+ D YIKTP  S FVKS+V+KGLLP IL  L+ ARKKAK EL  ETDPF+++VLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVTAFGR MI+ TK+ VE ++   NGY HDA V+YGDTDSVMC FGVSTV EAM LG+EAA  I+  F++PIKLEFEKVY+PYLLI+KKRYAG+Y+TK D  DKMDCKG+ETVRRDNC LVANL+NTCL+K+LI+RD   A+ +A+  I+DLLCNRIDISQL+I+KEL KT++EY +KQ H ELA +++KRD GSAPQLGDRVPYVI     K   Y K+EDPIYVL+NNI ID  YYL+NQL  PLLRIFEPI G++ A   LL GEH L++    S +G LS +T  +  CIGCK  L     ++C HC  +E A+Y +E     +LE +FSRLWT+CQRC+GSLH+++LC++RDCPIFY RKKVQK+L  Q+++++R  
Sbjct:  144 IYGYHGNTKSPYLKITMALPKLIAPAKRLLEQGFQFSEYPIHNYAGYESNIDFEIRFMVDTDVVGCNWIVLPAGQYRVRPRNNMKTRCQIESDVAWDQFVSHGPEGDWLKLAPMLTLSFDIECAGRKGIFPEPDKDPVIQIANMVIRQGEKEPFIRNVFTLKACAPIVGAQVLSFESEAELLKKWAEFIREVDPDVITGYNIQNFDLPYLLNRAAHLKVKNFDYLGRIKDIKSTVKEAMVQSKQMGKRENKIINMEGRVIFDLLQVLLRDYKLRSYTLNAVSFHFLEEQKEDVHHSIISDLQNGSDQTRRRLAVYCLKDAYLPLRLLDKLMSIINYIEMARVTGVSLGILLSRGQQIKVMSQLLRKAKTQDLVIPVYKVETGDEFTGATVIEPVRGYYDTPVATLDFASLYPSIMMAHNLCYTTLITTPSSIEKEGLSSDQYIKTPANSLFVKSTVRKGLLPEILESLLSARKKAKTELKSETDPFRKKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISGSVTAFGRLMIEQTKQFVEETYTVKNGYKHDAMVVYGDTDSVMCRFGVSTVAEAMELGQEAALEITKTFVSPIKLEFEKVYFPYLLINKKRYAGLYFTKADVHDKMDCKGIETVRRDNCQLVANLINTCLQKILINRDPMGAVDYAKQTISDLLCNRIDISQLVITKELAKTEDEYAAKQAHVELAHRMRKRDAGSAPQLGDRVPYVIIAAPKKTAAYLKSEDPIYVLENNIPIDVQYYLENQLAKPLLRIFEPILGEKKAESVLLRGEHTLTRTVVTSKIGGLSAFTKKRETCIGCKTPLDKAGVAVCKHCAASESALYQKEICELSALETKFSRLWTQCQRCTGSLHEDILCTSRDCPIFYMRKKVQKDLGHQEKVIQRFG 1048          

HSP 2 Score: 73.9442 bits (180), Expect = 2.111e-9
Identity = 40/118 (33.90%), Postives = 61/118 (51.69%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY--EIKETNPRS------VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ-TLKQRASQHQYAENLICKVEYCLKAS 547
            KW+R  +  + P    L+FQQLD+D Y  +     P S      + R +G   +G+S++ H+HG+ PY YV     F  EHCG+FR  LN+  +K   S      + +  V+Y +K S
Sbjct:   26 KWSRPLVTPLDPLKESLVFQQLDLDFYIGDPMSGMPGSKSGKVPIVRMFGITASGHSVVTHIHGYLPYFYVLAISNFKPEHCGKFRDALNKVVIKDMRSNKDNVSDAVLAVDYVMKES 143          
BLAST of DNA polymerase vs. TrEMBL
Match: A0A267EYR0 (DNA polymerase (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027421g6 PE=3 SV=1)

HSP 1 Score: 1215.68 bits (3144), Expect = 0.000e+0
Identity = 582/917 (63.47%), Postives = 711/917 (77.54%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMT-IPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKI---SFCQLEVDV-QWDKIICHSTEE-WPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEK--SICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 3455
            IYGF +N K+ FL+VTV +  L+A  KR+L +G + +   G H  + ++ +IDF +RFMVD+GL GC W+ELP   YT+R +  ++   S CQLE DV  W  ++ H  E  W  +AP+RILSFDIECAGR+G FP+ + DPVIQI+N++VR GE++PFI+N+FTL TCA IVG+ V+  KTE++LL  WS F+R++DPD+ITGYN QNFD+PYL+ RA  L V+ F  LGRI + +S I E+Q QSKQMGRR NK V+M GR+  DL  IL RD+KLRSYSLNAVS+HFL EQKEDV H IIT+LQNG+++TR RLAVYCLKD+ L LRLM+KLM +VNY+EMARVTGVPL++LLSRGQQ+KV+SQLLRKA  +D +LP  + + GDE+TGATVIEP+KG+Y  PIATLDF+SLYPSIMMAHNLCYTTLL  + SI ++ L  D+YIKTPCGSYF K +V+KGLLP IL QL+ ARK+AK +L  ETD FK++VLDGRQLALKISANSVYGFTGAQ G+LPCL+ISSSVTAFGR MI  TK  VE  +  ANGY HDAKVIYGDTDSVMC F  +TV +AM LG+EAA  IS KF  PIKLEFEKVY PYLLI+KKRYAG+Y+TKPD  DKMDCKG+ETVRRDN PLVANL+N CL K+LI RD + A+ HA+SVI+DLLCNR+DISQL+ISKELTKTD+EY  KQ H ELA KI+KRDPGSAPQLGDRVPYVI +       Y KAEDPIYVL+NN+ IDT+YYL+NQL NPLLRIFEPI G   A   LL GEH   K    S VG L+ +   +  C+GCK VL  D    ++C HC+PNE A+Y RE    + LE +F+RLWTECQRC GSLH++VLC++RDCPIFY RK++QK+L  QD ++ R  +   W
Sbjct:  222 IYGFHRNRKQPFLRVTVALQRLMAPAKRLLEQGFSWLQGRGSHCFSLYEANIDFEIRFMVDTGLVGCCWVELPAGKYTLREKENRLAPQSRCQLEADVASWSDVVAHPAEGVWSKVAPLRILSFDIECAGRRGVFPEPERDPVIQIANMVVRHGETDPFIRNVFTLGTCASIVGSQVLSFKTESDLLAAWSAFVRDVDPDVITGYNIQNFDMPYLLKRAATLKVQQFPFLGRISATKSTIREAQIQSKQMGRRENKWVNMEGRVQLDLLQILFRDYKLRSYSLNAVSYHFLHEQKEDVQHSIITELQNGNEQTRRRLAVYCLKDAYLPLRLMDKLMCLVNYMEMARVTGVPLTFLLSRGQQVKVISQLLRKAKEQDLLLPTQKVEQGDEYTGATVIEPVKGYYTTPIATLDFSSLYPSIMMAHNLCYTTLL-DQASIAKYSLQPDEYIKTPCGSYFAKKNVRKGLLPEILEQLLNARKRAKDDLKNETDEFKKRVLDGRQLALKISANSVYGFTGAQVGKLPCLDISSSVTAFGRTMIMETKRYVEERYTVANGYAHDAKVIYGDTDSVMCRFNAATVADAMKLGREAADYISSKFPPPIKLEFEKVYCPYLLINKKRYAGLYFTKPDKHDKMDCKGIETVRRDNSPLVANLVNACLHKILIDRDPDGAVQHAKSVISDLLCNRVDISQLVISKELTKTDDEYAGKQAHVELANKIRKRDPGSAPQLGDRVPYVIVSAAKGTPAYQKAEDPIYVLENNVPIDTNYYLENQLANPLLRIFEPILGDGKARSMLLSGEHTRVKAVVHSKVGGLAAFATRRAACLGCKAVLPQDAANGAVCKHCEPNESALYQREVGVLRVLEEKFNRLWTECQRCQGSLHEDVLCTSRDCPIFYMRKRLQKDLADQDALVGRFGEPYDW 1137          

HSP 2 Score: 73.559 bits (179), Expect = 3.326e-9
Identity = 35/90 (38.89%), Postives = 48/90 (53.33%), Query Frame = 2
Query:  221 KWARQNM-DVVKPEIMPLIFQQLDIDNYEIKETNPRS----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            KW R  +     P   PL+FQQ+D+D Y +      S    V R YG  + G+S++ HVHGF PY +V  P GF    C  F++ LNQ +
Sbjct:  107 KWRRAPLPHAADPATQPLVFQQMDLDCYTLPAAGSGSGASPVVRMYGITEAGHSVLVHVHGFNPYFFVPAPEGFSPADCHSFKQALNQAV 196          
BLAST of DNA polymerase vs. TrEMBL
Match: A0A293LKP1 (DNA polymerase (Fragment) OS=Ornithodoros erraticus OX=265619 PE=3 SV=1)

HSP 1 Score: 1215.68 bits (3144), Expect = 0.000e+0
Identity = 579/917 (63.14%), Postives = 710/917 (77.43%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEG-MTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQ------TEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 3455
            IYG+S   K+ FLKVT+ +P L+A  KR+L  G ++IP  G  +   +++++DF +R+MVDSG+ GCSWIELP  TY +R          K+S CQ+EVDV WD ++ H+ E +W  +AP RILSFDIECAGRKG FP+AD D VIQI+N++VR G  +PFI+N+FTLNTCAPI G  V+  K E ELL+ W++FIRE+DPDI+TGYN QNFD PYLINRA  L+V  F  LGRI+  R+ + +S  QSKQMGRR NK + M GR+ FDL L L+RD+KLRSY+LNAVS+HFL+EQKEDV H IITDLQNG D+TR RLAVYCLKD+ L LRL++KLM ++NY+EMARVTGVPL+YLL+RGQQIKV+SQLLRKAA +D V+P    + GD++TGATVIEP+KGFY  PI TLDF+SLYPSIMMAHNLCYTTLL  +   +   L  DD+I+TP G YFVKS ++KGLLP IL  L+ ARKKAK +L  ETDPF+++VLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVTAFGR MI+ TK  VE  + KANGY  D+KVIYGDTDSVM  FGV T+ EAM LGKEAA+ ++ KFI PI LEFEKVY+PYLLI+KKRYAG+ YT+PD++DKMDCKG+ETVRRDNCPLVANL+NTCL K+LI  D NAA+  A+  IADLLCNR+DISQL+I+KELTK D EY +KQ H ELA +++KRDPGSAP LGDRVPYVI         Y K+EDPIYVL NNI IDT YYL+NQL+ PLLR+FEPI G+  A   LL G+H  +K    S VGAL+ +T  +  CIGC+ VL D+  ++CAHCKP E  IY  E ++  SLE +F++LWT+CQRCSGSLH+EVLC++RDCPIFY R KVQK+L  QD+++ R     SW
Sbjct:  188 IYGYSDKGKQPFLKVTMVLPRLIAPAKRLLENGAISIPVCGPVSYKIYESNVDFEIRYMVDSGVVGCSWIELPAGTYRLRSSGGEDGTKRKVSRCQMEVDVAWDALVAHAPEGQWARVAPFRILSFDIECAGRKGIFPEADKDSVIQIANMVVRQGSKDPFIRNVFTLNTCAPIAGCEVLSFKREQELLQKWADFIREVDPDIVTGYNIQNFDFPYLINRAKHLSVSQFPFLGRIRDSRTLVKDSVLQSKQMGRRENKIITMEGRVQFDLLLALVRDYKLRSYTLNAVSYHFLQEQKEDVQHSIITDLQNGTDQTRRRLAVYCLKDAMLPLRLLDKLMCIINYMEMARVTGVPLTYLLTRGQQIKVVSQLLRKAAEQDLVMPTVHQETGDDYTGATVIEPVKGFYSVPITTLDFSSLYPSIMMAHNLCYTTLLDPRHKDK---LAADDFIRTPSGDYFVKSKLRKGLLPEILEDLLSARKKAKADLKKETDPFRKKVLDGRQLALKISANSVYGFTGAQVGKLPCLPISQSVTAFGRMMIEQTKREVEEKYTKANGYPADSKVIYGDTDSVMIRFGVDTLTEAMRLGKEAAEYVTSKFIRPINLEFEKVYFPYLLINKKRYAGLLYTRPDTYDKMDCKGIETVRRDNCPLVANLINTCLEKILIENDPNAAIDFAKQTIADLLCNRVDISQLVITKELTKNDGEYAAKQAHVELANRMRKRDPGSAPNLGDRVPYVIIAAAKGTAAYLKSEDPIYVLKNNIPIDTQYYLENQLSKPLLRVFEPILGETRAESVLLRGDHTRTKTVVHSKVGALAAFTKKRSTCIGCRAVLDDESAAVCAHCKPREAEIYLVEVQHQNSLETKFAKLWTQCQRCSGSLHEEVLCTSRDCPIFYMRMKVQKDLTDQDKVVSRFGAP-SW 1100          

HSP 2 Score: 81.2629 bits (199), Expect = 1.500e-11
Identity = 52/150 (34.67%), Postives = 77/150 (51.33%), Query Frame = 2
Query:   77 PSSKISKFEVKNNDED--DVSMDEWNHE---PEEICFEMESNTETV----KETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNYEIK--------ETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            PS    KF  +++D++  D  M+  + E    EE  ++ ES  E+V    ++T   ++WAR     +KP    L FQQLD+D+Y  +         T    + R +G    G+S++ HVHGF PY YVE P  F KEHC  F+  LN  +
Sbjct:   14 PSQGNKKFRSQDDDDEPMDFEMELASMEDMMSEEQTYDTESQ-ESVGQGPEDTCTSARWARPPAPRLKPSSQSLEFQQLDLDHYLGQPMDGMPGMRTGTVPIVRMFGVTDGGHSVLCHVHGFLPYFYVEAPNNFRKEHCRAFKDALNTAV 162          
BLAST of DNA polymerase vs. TrEMBL
Match: A0A267EYT1 (DNA polymerase (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027421g2 PE=3 SV=1)

HSP 1 Score: 1214.91 bits (3142), Expect = 0.000e+0
Identity = 582/917 (63.47%), Postives = 710/917 (77.43%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPH-YGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKI---SFCQLEVDV-QWDKIICHSTEE-WPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEK--SICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 3455
            IYGF +N K+ FL+VTV +  L+A  KR+L +G +     G H  + ++ +IDF +RFMVD+GL GC W+ELP   YT+R +  ++   S CQLE DV  W  ++ H  E  W  +AP+RILSFDIECAGR+G FP+ + DPVIQI+N++VR GE++PFI+N+FTL TCA IVG+ V+  KTE++LL  WS F+R++DPD+ITGYN QNFD+PYL+ RA  L V+ F  LGRI + +S I E+Q QSKQMGRR NK V+M GR+  DL  IL RD+KLRSYSLNAVS+HFL EQKEDV H IIT+LQNG+++TR RLAVYCLKD+ L LRLM+KLM +VNY+EMARVTGVPL++LLSRGQQ+KV+SQLLRKA  +D +LP  + + GDE+TGATVIEP+KG+Y  PIATLDF+SLYPSIMMAHNLCYTTLL  + SI ++ L  D+YIKTPCGSYF K +V+KGLLP IL QL+ ARK+AK +L  ETD FK++VLDGRQLALKISANSVYGFTGAQ G+LPCL+ISSSVTAFGR MI  TK  VE  +  ANGY HDAKVIYGDTDSVMC F  +TV +AM LG+EAA  IS KF  PIKLEFEKVY PYLLI+KKRYAG+Y+TKPD  DKMDCKG+ETVRRDN PLVANL+N CL K+LI RD + A+ HA+SVI+DLLCNR+DISQL+ISKELTKTD+EY  KQ H ELA KI+KRDPGSAPQLGDRVPYVI +       Y KAEDPIYVL+NN+ IDT+YYL+NQL NPLLRIFEPI G   A   LL GEH   K    S VG L+ +   +  C+GCK VL  D    ++C HC+PNE A+Y RE    + LE +F+RLWTECQRC GSLH++VLC++RDCPIFY RK++QK+L  QD ++ R  +   W
Sbjct:  220 IYGFHRNRKQPFLRVTVALQRLMAPAKRLLEQGFSWQQGRGSHCFSLYEANIDFEIRFMVDTGLVGCCWVELPAGKYTLREKENRLAPQSRCQLEADVASWSDVVAHPAEGVWSKVAPLRILSFDIECAGRRGVFPEPERDPVIQIANMVVRHGETDPFIRNVFTLGTCASIVGSQVLSFKTESDLLAAWSAFVRDVDPDVITGYNIQNFDMPYLLKRAATLKVQQFPFLGRISATKSTIREAQIQSKQMGRRENKWVNMEGRVQLDLLQILFRDYKLRSYSLNAVSYHFLHEQKEDVQHSIITELQNGNEQTRRRLAVYCLKDAYLPLRLMDKLMCLVNYMEMARVTGVPLTFLLSRGQQVKVISQLLRKAKEQDLLLPTQKVEQGDEYTGATVIEPVKGYYTTPIATLDFSSLYPSIMMAHNLCYTTLL-DQASIAKYSLQPDEYIKTPCGSYFAKKNVRKGLLPEILEQLLNARKRAKDDLKNETDEFKKRVLDGRQLALKISANSVYGFTGAQVGKLPCLDISSSVTAFGRTMIMETKRYVEERYTVANGYAHDAKVIYGDTDSVMCRFNAATVADAMKLGREAADYISSKFPPPIKLEFEKVYCPYLLINKKRYAGLYFTKPDKHDKMDCKGIETVRRDNSPLVANLVNACLHKILIDRDPDGAVQHAKSVISDLLCNRVDISQLVISKELTKTDDEYAGKQAHVELANKIRKRDPGSAPQLGDRVPYVIVSAAKGTPAYQKAEDPIYVLENNVPIDTNYYLENQLANPLLRIFEPILGDGKARSMLLSGEHTRVKAVVHSKVGGLAAFATRRAACLGCKAVLPQDAANGAVCKHCEPNESALYQREVGVLRVLEEKFNRLWTECQRCQGSLHEDVLCTSRDCPIFYMRKRLQKDLADQDALVGRFGEPCDW 1135          

HSP 2 Score: 72.7886 bits (177), Expect = 4.627e-9
Identity = 35/90 (38.89%), Postives = 48/90 (53.33%), Query Frame = 2
Query:  221 KWARQNM-DVVKPEIMPLIFQQLDIDNYEIKETNPRS----VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            KW R  +     P   PL+FQQ+D+D Y +      S    V R YG  + G+S++ HVHGF PY +V  P GF    C  F++ LNQ +
Sbjct:  105 KWRRAPLPHAADPATQPLMFQQMDLDCYTLPAAGSGSGASPVVRMYGITEAGHSVLVHVHGFNPYFFVPAPEGFSPADCHSFKQALNQAV 194          
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:103023852])

HSP 1 Score: 1151.73 bits (2978), Expect = 0.000e+0
Identity = 548/917 (59.76%), Postives = 700/917 (76.34%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE----------KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+  +    FL++T+ +P LVA  KR+L +G    ++   + + ++ +IDF +RFMVD  + GC WIELP   Y +R +            K+S CQ EVDV W+ +I H  E EW  IAP+R+LSFDIECAGRKG FP+A++DPVIQI++++ R GE EPFI+ +FTL +CA IVG+ ++    E +LL+ W+ F+R +DPDIITGYN QNFDLPYL+NRA  L V  F  LGR++ ++S + +S  QSKQMGRR NK V+M GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ KLM V+NY+EMARVTGVPL+YLLSRGQQIKV+SQLLR+A  +D V+P  +++ G+++TGATVIEP KG+Y  PIATLDF+SLYPSIMMAHNLCYTTLL+  ++ +  GL+ +D+I+TP G  FVKSSV+KGLLP IL  L+ ARK+AK EL  ETDPFK+QVLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +  ANGY  DAKV+YGDTDSVM   GV TV EAM +G+EAA+ +S  F+ PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  +  DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  +Q H ELA++++KRD GSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL+ PL+RIFEPI G+  A   LL G+H   K    S VG L  +   +  CIGC+ VL+ D  ++C  CK  E  +Y +E  +  +LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q +++ R  
Sbjct:  190 NMYGYHGSRPLDFLRITMAMPRLVAPAKRLLEQGFKFANFSTQSYSPYEANIDFEIRFMVDCDVVGCCWIELPKGKYKLREERTTKDTDSRHPGKVSLCQYEVDVGWNDLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEAEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKQLLQSWAEFVRTVDPDIITGYNIQNFDLPYLLNRAAALKVNLFPYLGRVRGIKSILKDSSFQSKQMGRRENKTVNMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYTGATVIEPEKGYYSIPIATLDFSSLYPSIMMAHNLCYTTLLQKGQADK-LGLSAEDFIRTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESKYTIANGYKADAKVVYGDTDSVMVKLGVDTVQEAMTVGREAAEWVSSHFVPPIKLEFEKVYHPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPDGAVAHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGRQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLSKPLMRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-AAVCDFCKKKESELYQKEIAHLSALEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQGKLVSRFG 1104          

HSP 2 Score: 76.6406 bits (187), Expect = 1.338e-13
Identity = 49/139 (35.25%), Postives = 66/139 (47.48%), Query Frame = 2
Query:  137 DEWNHEPEEICF--EMESNTET---------VKETDEFS-----KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            D  +H  EE+    EME + E+         +   D FS     +W R     +KP+   LIFQQ+D+D Y          +++   P  V R +G   NGNS+  HVHGF PY YV  P GF   H  EF+K LN  +
Sbjct:   29 DGPSHFEEELALFDEMEMDAESGEGQAGHDVIPVGDLFSADLNPRWRRPTAPTIKPDKDTLIFQQIDLDYYLGSAVAGMPGQVQGKVP--VVRMFGVTDNGNSVCCHVHGFAPYFYVPAPAGFTAAHLSEFQKELNSAV 165          
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:103023852])

HSP 1 Score: 1151.73 bits (2978), Expect = 0.000e+0
Identity = 548/917 (59.76%), Postives = 700/917 (76.34%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE----------KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+  +    FL++T+ +P LVA  KR+L +G    ++   + + ++ +IDF +RFMVD  + GC WIELP   Y +R +            K+S CQ EVDV W+ +I H  E EW  IAP+R+LSFDIECAGRKG FP+A++DPVIQI++++ R GE EPFI+ +FTL +CA IVG+ ++    E +LL+ W+ F+R +DPDIITGYN QNFDLPYL+NRA  L V  F  LGR++ ++S + +S  QSKQMGRR NK V+M GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ KLM V+NY+EMARVTGVPL+YLLSRGQQIKV+SQLLR+A  +D V+P  +++ G+++TGATVIEP KG+Y  PIATLDF+SLYPSIMMAHNLCYTTLL+  ++ +  GL+ +D+I+TP G  FVKSSV+KGLLP IL  L+ ARK+AK EL  ETDPFK+QVLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +  ANGY  DAKV+YGDTDSVM   GV TV EAM +G+EAA+ +S  F+ PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  +  DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  +Q H ELA++++KRD GSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL+ PL+RIFEPI G+  A   LL G+H   K    S VG L  +   +  CIGC+ VL+ D  ++C  CK  E  +Y +E  +  +LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q +++ R  
Sbjct:  207 NMYGYHGSRPLDFLRITMAMPRLVAPAKRLLEQGFKFANFSTQSYSPYEANIDFEIRFMVDCDVVGCCWIELPKGKYKLREERTTKDTDSRHPGKVSLCQYEVDVGWNDLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEAEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKQLLQSWAEFVRTVDPDIITGYNIQNFDLPYLLNRAAALKVNLFPYLGRVRGIKSILKDSSFQSKQMGRRENKTVNMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYTGATVIEPEKGYYSIPIATLDFSSLYPSIMMAHNLCYTTLLQKGQADK-LGLSAEDFIRTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESKYTIANGYKADAKVVYGDTDSVMVKLGVDTVQEAMTVGREAAEWVSSHFVPPIKLEFEKVYHPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPDGAVAHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGRQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLSKPLMRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-AAVCDFCKKKESELYQKEIAHLSALEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQGKLVSRFG 1121          

HSP 2 Score: 76.6406 bits (187), Expect = 1.216e-13
Identity = 48/136 (35.29%), Postives = 65/136 (47.79%), Query Frame = 2
Query:  146 NHEPEEICF--EMESNTET---------VKETDEFS-----KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            +H  EE+    EME + E+         +   D FS     +W R     +KP+   LIFQQ+D+D Y          +++   P  V R +G   NGNS+  HVHGF PY YV  P GF   H  EF+K LN  +
Sbjct:   49 SHFEEELALFDEMEMDAESGEGQAGHDVIPVGDLFSADLNPRWRRPTAPTIKPDKDTLIFQQIDLDYYLGSAVAGMPGQVQGKVP--VVRMFGVTDNGNSVCCHVHGFAPYFYVPAPAGFTAAHLSEFQKELNSAV 182          
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:103023852])

HSP 1 Score: 1077.39 bits (2785), Expect = 0.000e+0
Identity = 524/919 (57.02%), Postives = 677/919 (73.67%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQ---TEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAE---------DPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+  +    FL++T+ +P LVA  KR+L +G    ++   + + ++ +IDF +RFMVD  + GC WIELP   Y +R +    +  S    +VDV W+ +I H  E EW  IAP+R+LSFDIECAGRKG FP+A++DPVIQI++++ R GE EPFI+ +FTL +CA IVG+ ++    E +LL+ W+ F+R +DPDIITGYN QNFDLPYL+NRA  L V  F  LGR++ ++S + +S  QSKQMGRR NK V+M GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ KLM V+NY+EMARVTGVPL+YLLSRGQQIKV+SQLLR+A  +D V+P  +++ G+++TG +             +TLDF+SLYPSIMMAHNLCYTTLL+  ++ +  GL+ +D+I+TP G  FVKSSV+KGLLP IL  L+ ARK+AK EL  ETDPFK+QVLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +  ANGY  DAKV+YGDTDSVM   GV TV EAM +G+EAA+ +S  F+ PIKLEFEKVY+PYLLI+KKRYAG+Y+ +  +  DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD + A++HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  +Q H ELA++++KRD GSAP LGDRVPYVI         Y K+E         DPIY           YYL+ QL+ PL+RIFEPI G+  A   LL G+H   K    S VG L  +   +  CIGC+ VL+ D  ++C  CK  E  +Y +E  +  +LE +FSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q +++ R  
Sbjct:  183 NMYGYHGSRPLDFLRITMAMPRLVAPAKRLLEQGFKFANFSTQSYSPYEANIDFEIRFMVDCDVVGCCWIELPKGKYKLREERTTKDTDSRHPGKVDVGWNDLISHPAEGEWQRIAPLRVLSFDIECAGRKGVFPEAEVDPVIQIASMVQRQGEKEPFIRTVFTLQSCASIVGSQILCFTQEKQLLQSWAEFVRTVDPDIITGYNIQNFDLPYLLNRAAALKVNLFPYLGRVRGIKSILKDSSFQSKQMGRRENKTVNMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLTYLLSRGQQIKVVSQLLRQAMKQDLVMPVVKTEGGEDYTGVST---------AVFSTLDFSSLYPSIMMAHNLCYTTLLQKGQADK-LGLSAEDFIRTPTGDLFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIEQTKQLVESKYTIANGYKADAKVVYGDTDSVMVKLGVDTVQEAMTVGREAAEWVSSHFVPPIKLEFEKVYHPYLLINKKRYAGLYFSSSAEHHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPDGAVAHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGRQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGVAAYMKSEVGSVFLYESDPIY-----------YYLEQQLSKPLMRIFEPILGESKAESVLLKGDHTRCKTVLTSRVGGLMAFAQKRSTCIGCRAVLKTD-AAVCDFCKKKESELYQKEIAHLSALEEKFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQGKLVSRFG 1079          

HSP 2 Score: 78.9518 bits (193), Expect = 2.733e-14
Identity = 46/129 (35.66%), Postives = 64/129 (49.61%), Query Frame = 2
Query:  146 NHEPEEICF----EMESNTETVKETDEFS-----KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            +H  EE+      EM++ +   +  D FS     +W R     +KP+   LIFQQ+D+D Y          +++   P  V R +G   NGNS+  HVHGF PY YV  P GF   H  EF+K LN  +
Sbjct:   32 SHFEEELALFDEMEMDAESGEGQAGDLFSADLNPRWRRPTAPTIKPDKDTLIFQQIDLDYYLGSAVAGMPGQVQGKVP--VVRMFGVTDNGNSVCCHVHGFAPYFYVPAPAGFTAAHLSEFQKELNSAV 158          
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55])

HSP 1 Score: 304.294 bits (778), Expect = 2.455e-84
Identity = 245/851 (28.79%), Postives = 402/851 (47.24%), Query Frame = 3
Query: 1065 IRILSFDIECAGRKGSFPDADIDPVIQI-----SNIIVRFGESEPFI---------KNIFTLNT--CAP------IVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDL-------VLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDF--VLPDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIR-----EFGLTE------------DDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKREL-AIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVIT--TLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQ 3443
            + +++ ++    R+   PD + DP+  +     S+  +   +S  F          K +FT ++   AP      + G  V     E EL E   N +R  DPDI+ GY  Q     YL+ RA+ L V     L R+    +K     +   + G      +H+ GRI  +L+ I+  +  L +YS   V+FH L ++    + + ++D  +      H+  +Y  K  D        V++L+ +  +V    E ARV G+   ++L+RG Q +V S +LR A   ++  V P  Q +           V+EP   FY   +  LDF SLYPSI++A+N C++T L   ES+       FG T             +D   +P G  FVK+SV+KG+LP +L ++++ R   K  + +   D    ++LD RQL LK+ AN  +G+T A   GR+P + +  S+    RE ++   ++V    N+   +   A+V+YGDTDS+  L   +T  +A  +G E A++++     PIKL+FEKVY P +L +KKRY G  Y   D  D + D KG+ETVRRD CP VA +L   ++ L  +RD +    + Q     +L  +  +  L  +KE  +    Y      P  EL +++   D    P++G+RVPYVI   T GV   +      P+ VL D ++ ++  YY+  Q+  P+ RIF  I     +  + L      S   +    G +S+Y      C  C  +    +  +C  C+     +     +  +  E +  +L   C+ CSGS+ ++V C + DCP+ Y   +V ++L     + + L Q
Sbjct: 2092 LTVMTMELHARTRRDLEPDPEFDPICALFYCLSSDSTLPDSDSMQFTGAVIIDKDCKLVFTQSSRSTAPLLVRSGVAGLQVSYAAEEKELFEEVCNIMRRYDPDILVGYEVQMRSWGYLLQRASALGVDLCQQLSRVPD--AKENRFTADKDEYGADTMSEIHIVGRITINLWRIMKTEAALNNYSFENVAFHLLHQRFPLYSPRTLSDWFD------HKTHLYRWKVVDHYVSRVCGVVQLLRQQDIVGRTSEFARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAPQCIPLVMEPESRFYSNSVVVLDFQSLYPSIVIAYNYCFSTCLGHVESLGTSDEFRFGCTSLRVPPDLLYQLRNDITVSPNGIAFVKASVRKGVLPRMLEEILQTRFMVKGVMKSYRDDKALHRLLDARQLGLKLIANVTFGYTSANFSGRMPSVEVGDSIVHKARETLERAIKLV----NETKKW--GARVVYGDTDSMFVLLKGATKEQAFKIGHEIAEAVTATNPKPIKLKFEKVYLPCVLQTKKRYVGYMYETVDQKDPVFDAKGIETVRRDGCPAVAKILERSVKLLFETRDISLVKQYVQRQCVKVLEAKASMQDLTFAKEY-RGSGSYRPGACIPALELTRRMMSYDRRLEPRVGERVPYVIVYGTPGV--PLIQLVRRPLEVLQDPSLRLNATYYITKQILPPVARIFSLIGVDVFSWYQELPRSATTSFRAEVGRKGTISQYFTTL-HCPVCDELT---QLGVCERCRAEPQRVVVTLQQNLRQWESQQDQLLKICRACSGSVERQVPCVSLDCPVLYKVTRVNRQLSRAPYLRQLLEQ 2921          
BLAST of DNA polymerase vs. Ensembl Cavefish
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-050302-55])

HSP 1 Score: 300.056 bits (767), Expect = 4.958e-83
Identity = 245/852 (28.76%), Postives = 396/852 (46.48%), Query Frame = 3
Query: 1065 IRILSFDIECAGRKGSFPDADIDPVIQI-----SNIIVRFGESEPFIKNIFTLNTC----------AP------IVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDL-------VLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDF--VLPDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIR-----EFGLTE------------DDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKREL-AIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVIT--TLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFE-------------PIFGKELATRKLLHGEHVLSKFKQQSNVGALSKY-TVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKEL 3407
            + +++ ++    R+   PD + DP+  +     S+  +   +S  F   +     C          AP      + G  V     E EL E   N +R  DPDI+ GY  Q     YL+ RA+ L V     L R+    +K     +   + G      +H+ GRI  +L+ I+  +  L +YS   V+FH L ++    + + ++D  +      H+  +Y  K  D        V++L+ +  +V    E ARV G+   ++L+RG Q +V S +LR A   ++  V P  Q +           V+EP   FY   +  LDF SLYPSI++A+N C++T L   ES+       FG T             +D   +P G  FVK+SV+KG+LP +L ++++ R   K  + +   D    ++LD RQL LK+ AN  +G+T A   GR+P + +  S+    RE ++   ++V    N+   +   A+V+YGDTDS+  L   +T  +A  +G E A++++     PIKL+FEKVY P +L +KKRY G  Y   D  D + D KG+ETVRRD CP VA +L   ++ L  +RD +    + Q     +L  +  +  L  +KE  +    Y      P  EL +++   D    P++G+RVPYVI   T GV   +      P+ VL D ++ ++  YY+  Q+  P+ RIF              P   K         GE V  K       G +S+Y T +   C  C  +    +  +C  C+     +     +  +  E +  +L   C+ CSGS+ ++V C + DCP+ Y   +V ++L
Sbjct: 2036 LTVMTMELHARTRRDLEPDPEFDPICALFYCLSSDSTLPDSDSMQFTGAVIIDKDCHSAGQGSRSTAPLLVRSGVAGLQVSYAAEEKELFEEVCNIMRRYDPDILVGYEVQMRSWGYLLQRASALGVDLCQQLSRVPG-DAKENRFTADKDEYGADTMSEIHIVGRITINLWRIMKTEAALNNYSFENVAFHLLHQRFPLYSPRTLSDWFD------HKTHLYRWKVVDHYVSRVCGVVQLLRQQDIVGRTSEFARVFGIQFYHVLTRGSQYRVESMMLRVAKPMNYIPVTPSTQQRAQQRAPQCIPLVMEPESRFYSNSVVVLDFQSLYPSIVIAYNYCFSTCLGHVESLGTSDEFRFGCTSLRVPPDLLYQLRNDITVSPNGIAFVKASVRKGVLPRMLEEILQTRFMVKGVMKSYRDDKALHRLLDARQLGLKLIANVTFGYTSANFSGRMPSVEVGDSIVHKARETLERAIKLV----NETKKW--GARVVYGDTDSMFVLLKGATKEQAFKIGHEIAEAVTATNPKPIKLKFEKVYLPCVLQTKKRYVGYMYETVDQKDPVFDAKGIETVRRDGCPAVAKILERSVKLLFETRDISLVKQYVQRQCVKVLEAKASMQDLTFAKEY-RGSGSYRPGACIPALELTRRMMSYDRRLEPRVGERVPYVIVYGTPGV--PLIQLVRRPLEVLQDPSLRLNATYYITKQILPPVARIFSLIGVDVFSWYQELPRIQKSSCPSGGTVGEEVGRK-------GTISQYFTTL--HCPVCDELT---QLGVCERCRAEPQRVVVTLQQNLRQWESQQDQLLKICRACSGSVERQVPCVSLDCPVLYKVTRVNRQL 2859          
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Match: pold1 (polymerase (DNA directed), delta 1, catalytic subunit [Source:ZFIN;Acc:ZDB-GENE-060429-1])

HSP 1 Score: 1126.31 bits (2912), Expect = 0.000e+0
Identity = 553/888 (62.27%), Postives = 675/888 (76.01%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE------KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKK--AKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNIS-SSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEK-SICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCS 3356
            IYGF  N K  FLKVTV +P LVA  KR+L +G        H+  SF+ +IDF +RFMVD  + GC+WIE+P   Y +R ++       K+S  QLEVDV     + H TE EW +IAP+R+LSFDIECAGRKG FP+ + DPVIQISN+++R GE +PF++N+FTLNTCAPIVG  V   + E E+L+ W+ FIR +DPDIITGYN QNFDLPYL+NRA+ L V  FS LGRI SMRS I ES  QSKQMGRR NK +++ GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H IITDLQNG+++TR RLAVYCLKD+ L LRL+ KLM V+NY+EMARVTGVPLSYLL+RGQQIKV+SQLLR+A  +D +LP  + +  +++ GATVIEPIKG+Y  PIATLDF+SLYPSIMMAHNLCYTTLL+ K  +   GL+ +D+IKTP G +FVKSSV+KGLLP IL  L+ ARKK  AK EL  ETDPFK++VLDGRQLALKISANSVYGFTGAQ G+LPCL IS  SVT FGR+MI+ TK +VES +   NG   DAKVIYGDTDSVM  F V TV E M +G+EAA  +S  FI PIKLEFEKVYYPYLLI+KKRYAG+Y+ +  ++ DKMDCKG+ETVRRDN PLVANL+NTCL+K+LI RD + A+ HA+  I+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRDPGSAP LGDRVPYVI         Y K+EDPIYVL+NNI IDT YYL+ QL  PLLRIFEPI G+  A   LL GEH   K    S VG L  +   +  CIGCK VL++D   ++C +CK  E  +Y +E  +  ++E +FSRLWT+CQRC GSLH++VLC+
Sbjct:  203 IYGFHGNQKSNFLKVTVALPRLVAAAKRLLTQGFRFDSMPEHSYLSFEANIDFEIRFMVDHKVVGCNWIEIPAGKYQLREESASGKLPGKMSLVQLEVDVAVTDFVSHPTEGEWQAIAPLRVLSFDIECAGRKGIFPEPEKDPVIQISNMVLRQGERDPFVRNVFTLNTCAPIVGCEVHSFQHEGEMLKAWAEFIRIVDPDIITGYNIQNFDLPYLLNRASTLRVSTFSFLGRITSMRSVIKESSFQSKQMGRRDNKVINLEGRVTFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIITDLQNGNEQTRRRLAVYCLKDAMLPLRLLEKLMCVINYMEMARVTGVPLSYLLTRGQQIKVVSQLLRQAMKQDLLLPVVKPEGNEDYAGATVIEPIKGYYTVPIATLDFSSLYPSIMMAHNLCYTTLLQ-KGVVENLGLSAEDFIKTPSGDHFVKSSVRKGLLPEILENLLSARKKRRAKEELKNETDPFKQKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQQSVTGFGRQMIETTKNLVESRYTTENGQKADAKVIYGDTDSVMVKFSVKTVAEGMEMGREAAAWVSSHFIPPIKLEFEKVYYPYLLINKKRYAGLYFSSSSETHDKMDCKGIETVRRDNSPLVANLINTCLQKILIDRDPDGAVDHAKDTISDLLCNRIDISQLVITKELTRTADEYAGKQAHVELAERMRKRDPGSAPNLGDRVPYVIVKATKGVAAYMKSEDPIYVLENNIPIDTQYYLEQQLAKPLLRIFEPILGENKAESILLKGEHTRCKTVLTSKVGGLMAFAKKRSTCIGCKAVLKNDAAGAVCDYCKKQESELYQKEVLHLNAMEEKFSRLWTQCQRCQGSLHEDVLCT 1089          

HSP 2 Score: 68.5514 bits (166), Expect = 8.790e-12
Identity = 39/121 (32.23%), Postives = 57/121 (47.11%), Query Frame = 2
Query:  218 SKWARQNMDVVKPEIMPLIFQQLDIDNYEIKETNPRS----------VFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKIL-NQTLKQRASQHQYAENLICKVEYCLKAS 547
            SKW R ++  +  +   L+FQQ+++D+Y  K  +             V R +G    GNS+  HVHGF PY YV    GF  EH   F++ L N  +K   S        +  V+ C+K S
Sbjct:   82 SKWLRPSLPKIDQKTESLVFQQIELDHYIGKGFHVSGMPGATQSQVPVVRMFGITNKGNSLCCHVHGFAPYFYVAAQTGFKSEHLVSFKRELNNAVIKDMRSNKDNLSQAVLAVDICVKES 202          
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009864.1 (pep scaffold:Pmarinus_7.0:GL480102:12343:24944:1 gene:ENSPMAG00000008922.1 transcript:ENSPMAT00000009864.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 181.415 bits (459), Expect = 2.416e-49
Identity = 137/469 (29.21%), Postives = 233/469 (49.68%), Query Frame = 3
Query: 2079 VKSSVKKGLLPNILIQLIEARKKAKRELA-IETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVH-DAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKEL-TKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFEPIFGKEL--ATRKLLHGEHVLSKFKQQSNVGALSKYTVIK----PKCIGCKNVLRDDEKSICAHCK--PNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQ 3443
            V++SV+KG+LP +L ++++ R   K  +     D    ++L  RQL LK+ AN  +G+T A   GR+PC+         G  ++   +E +E +  + N      A+V+YGDTDS+  L   ++  +A   GKE A +++    +PIKL+FEKVY P +L +KKRY G  Y   +  D + D KG+ETVRRD CP VA +L   ++ L   +D +    + Q     +L  R  +  LI +KE   ++  +  +  P   +A+++   D  S P+ G+RVPYVI        +      P  +L + ++ I+  YY+  Q+  PL R+   + G ++    ++L     V +   Q S  G   K T+++      C  C ++ +D    IC  C+  P E+A+   +  +++  E R ++L   C+ C+G    +  C + DCP  +     +K L     I   ++Q
Sbjct:    3 VQASVRKGVLPRMLEEILQTRIMVKHCMKDYRGDGALTRLLHARQLGLKLIANVTFGYTAAHFSGRMPCVE-----EQVGDSIVHKARETLERAIKQVNETKEWGARVVYGDTDSMFVLLEGASKEQAFRTGKEIAAAVTAANPSPIKLKFEKVYLPCVLQTKKRYVGYMYESLEQKDPVYDAKGIETVRRDGCPAVAKVLERSIKLLFDEKDISRVKQYVQRQCMKILEGRASMQDLIFAKEYRGRSGYKPGACVPALHVARRMFACDRRSEPRAGERVPYVIVHGAPGLPLIQLVRRPQELLQEPSLLINAPYYVSKQILPPLDRLLS-LIGVDVFRWYQQLPRSVRVWAAHHQSSAEG--RKATIVQYFSTVNCPVCGDITKD---GICGQCRQQPQEVAVLLLQQIHSQ--ERRQAQLLQICRSCTGHAEWKPPCCSTDCPTLFRLLVTEKGLCQASYIRSLIDQ 458          
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006446.1 (pep scaffold:Pmarinus_7.0:GL477096:156518:193524:1 gene:ENSPMAG00000005826.1 transcript:ENSPMAT00000006446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 111.694 bits (278), Expect = 9.899e-27
Identity = 92/349 (26.36%), Postives = 153/349 (43.84%), Query Frame = 3
Query: 1056 IAPIRILSFDIECAGRKGSFPDADIDPVI------------------QISNIIVR---FGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQ-SQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITD-LQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGAT----VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESI 2021
            I  + ++S ++    RK   PD ++DPV                    ++ +IV+    G +         L   + + G  VI    E +L       +   DPDI+ GY  Q     YL+ RA  L     S + R   M+ + TE+Q    K        ++H+ GR+  +L+  +  +  L  YS   V++H L E+      + ++D   N     R ++  Y +      ++L+N+L  +    E+AR+ G+    +LSRG Q +V S +LR A   +++      +       A     V+EP   FY  P+  LDF SLYPSI++A+N CY+T L   E +
Sbjct:    2 IQHLTLMSMEMHARSRKHLVPDPNLDPVCCIFYHIMCDAPLPGRDANDVTGLIVQDAAHGTAPEDSSKDAGLPARSEVSGLDVIHVSKEDQLFHELEQIVHRFDPDILLGYEVQMLSWGYLLQRAAALKRDFKSTISR---MKVEPTETQVGVGKNEEDDDFPDIHIGGRVVLNLWSQMRSEVTLNIYSFENVAYHVLHERYPLFNFRTLSDWFDNKTGPFRWKMVDYYVCRVVGNIKLLNQLDFIGRTSELARLFGIQFHEVLSRGSQFRVESMMLRVAKPLNYIAVSPSVQQRAHMRAAECLPLVMEPESRFYSSPVLVLDFQSLYPSIIIAYNYCYSTCLGRVEHL 347          
BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002970.1 (pep scaffold:Pmarinus_7.0:GL476977:35626:117313:1 gene:ENSPMAG00000002616.1 transcript:ENSPMAT00000002970.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.6766 bits (117), Expect = 4.547e-6
Identity = 69/340 (20.29%), Postives = 137/340 (40.29%), Query Frame = 3
Query: 1071 ILSFDIECAGRKGSFPDADIDPVIQISNIIVRFG----ESEPFIKNI--FTLNTCAPIVGAHVIPHKT-EAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLS--YLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLD---FASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYI 2054
            +L+FDIE       FPDA+ D ++ IS ++   G      E   ++I  F         G   I ++  EA LL+ + + + E  P+I   YN  +FD P++  RA    +      G  K  + +   +Q+      R + ++ ++P           +  H L++ +   + +  +E   E++      + Q         LA Y + D+     +    M  V+    A  T +P+    +L +G      + L+ +A   + V P+ Q ++ +  TG   +   + +    +  L+   F S  P     +   +  L++  E+  +  L E++ I
Sbjct:  257 VLAFDIETTKLPLKFPDAETDQIMMISYMVDGQGYLITNREVVSEDIEDFEFTPKPEYEGPFTIFNEADEAGLLQRFFDHLHETKPNIFVTYNGDSFDWPFVERRALAHGLDLLIETGFQKDSQGEYKATQALHMDCLRWVKRDSYLP-----------VGSHNLKAAAKAKLGYDPVELDPEEMCRMAAEEAQT--------LATYSVSDAVATYYM---YMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIVFPNKQQEEFNRLTGDGHVLDSETYVGGHVEALECGVFRSDIPCRFKMNPGVFEELMEKVETTLKHALEEEEKI 574          
BLAST of DNA polymerase vs. Ensembl Yeast
Match: POL3 (Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) [Source:SGD;Acc:S000002260])

HSP 1 Score: 948.732 bits (2451), Expect = 0.000e+0
Identity = 479/914 (52.41%), Postives = 629/914 (68.82%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSK-DGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGL-TEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLS--KFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 3455
            I+G+S + K  F K+ V  P +V   +     G    +  + N  +   +I +++R MVD G+ G SWI LP   Y++     ++S CQLEV + +  +I H  E +W   AP+RI+SFDIECAGR G FP+ + DPVIQI+N++   G  +PFI+N+FTLNTC+PI G+ +  H TE E+L +W NFI ++DPD+I GYNT NFD+PYL+NRA  L V  F   GR+K+++ +I ES   SK  G R  KNV++ GR+  DL   + R++KLRSY+LNAVS HFL EQKEDV + II+DLQNGD  TR RLAVYCLKD+ L LRLM KLM +VNY EMARVTGVP SYLL+RGQQIKV+SQL RK    D V+P+ QS+   D++ GATVIEPI+G+YD PIATLDF SLYPSIMMAHNLCYTTL  +K ++    L  ++DY+ TP G YFV +  ++G+LP IL +LI ARK+AK++L  E DPFKR VL+GRQLALKISANSVYGFTGA  G+LPCL ISSSVTA+GR MI  TK  V+  +   NGY HDA V+YGDTDSVM  FG + + EAM LG EAA+ +S  F  PI LEFEK Y+PYLLI+KKRYAG+++T PD FDK+D KG+ +VRRD+C LV+ ++N  L+K+LI R+ + AL+  +  I D+L NR+DIS+LIISK L      YT+ QPH+ LA+++K+R+ G  P +GDRV YVI  +G  DK+Y +AEDP++VL+NNI +D+ YYL NQL NP++ I  PI G + A     +G  V+   K    S  G L  +      C  CK  LR  E  +C++C      +Y +     + LE ++SRLWT+CQRC+G+LH EVLCSN++C IFY R KV+KEL       E++ Q   W
Sbjct:  202 IWGYSGDTKLPFWKIYVTYPHMVNKLRTAFERGHLSFNSWFSNGTTTYDNIAYTLRLMVDCGIVGMSWITLPKGKYSMIEPNNRVSSCQLEVSINYRNLIAHPAEGDWSHTAPLRIMSFDIECAGRIGVFPEPEYDPVIQIANVVSIAGAKKPFIRNVFTLNTCSPITGSMIFSHATEEEMLSNWRNFIIKVDPDVIIGYNTTNFDIPYLLNRAKALKVNDFPYFGRLKTVKQEIKESVFSSKAYGTRETKNVNIDGRLQLDLLQFIQREYKLRSYTLNAVSAHFLGEQKEDVHYSIISDLQNGDSETRRRLAVYCLKDAYLPLRLMEKLMALVNYTEMARVTGVPFSYLLARGQQIKVVSQLFRKCLEIDTVIPNMQSQASDDQYEGATVIEPIRGYYDVPIATLDFNSLYPSIMMAHNLCYTTLC-NKATVERLNLKIDEDYVITPNGDYFVTTKRRRGILPIILDELISARKRAKKDLRDEKDPFKRDVLNGRQLALKISANSVYGFTGATVGKLPCLAISSSVTAYGRTMILKTKTAVQEKYCIKNGYKHDAVVVYGDTDSVMVKFGTTDLKEAMDLGTEAAKYVSTLFKHPINLEFEKAYFPYLLINKKRYAGLFWTNPDKFDKLDQKGLASVRRDSCSLVSIVMNKVLKKILIERNVDGALAFVRETINDILHNRVDISKLIISKTLAPN---YTNPQPHAVLAERMKRRE-GVGPNVGDRVDYVI--IGGNDKLYNRAEDPLFVLENNIQVDSRYYLTNQLQNPIISIVAPIIGDKQA-----NGMFVVKSIKINTGSQKGGLMSFIKKVEACKSCKGPLRKGEGPLCSNCLARSGELYIKALYDVRDLEEKYSRLWTQCQRCAGNLHSEVLCSNKNCDIFYMRVKVKKEL------QEKVEQLSKW 1097          
BLAST of DNA polymerase vs. Ensembl Yeast
Match: REV3 (Catalytic subunit of DNA polymerase zeta; involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; forms a complex with Rev7p, Pol31p and Pol32p [Source:SGD;Acc:S000006088])

HSP 1 Score: 288.5 bits (737), Expect = 1.518e-80
Identity = 235/874 (26.89%), Postives = 400/874 (45.77%), Query Frame = 3
Query: 1074 LSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPF------------IKNIFTLNTCAPIVGAH------VIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSL---LGRIK-SMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAV--YCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTG----ATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTED-------------------DYIKTPCGSYFVKSSVKKGLLPNILIQLIEAR---KKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPD-SFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKR---DPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDN-NIGIDTDYYLKNQLTNPLLRIFEPI------FGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEI---------RFSRLWTECQRCSGSL---------HQEVLCSNRDCPIFYTRKKVQKEL-----ISQDEIMERLN 3440
            L+ +I    R    PD  ID V    ++I+   E E F            I +  + ++  P    H      V+ +++E E+ E  ++ +  +DPDI++G+   NF   Y+I R  K++   F +   L R+K  +++K++++   +   G      + + GR   +++  L  D  L  Y++ + +F+ L ++    + + +T++ N    T     V  Y L  + + ++L+ K   +   +E AR+ G+    +  RG Q KV S L+R    E F+L     KD  +         V+EP   FY  P+  LDF SLYPSIM+ +N CY+T++     +RE  LTE+                   D    P G  + K+SV+K  L  +L  +++ R   KK   E+  +    KR +L+ +QLALK+ AN  YG+T A   GR+PC +++ S+   GRE ++   +++E           +AKV+YGDTDS+       T +EA ++G   A+ ++     PI L+FEKVY+P +LISKKRY G  Y  P  +    D KG+ETVRRD  P    ++  C+R L  ++D +    + Q+    +   ++       +KE+      Y S++     A  +K+R   D  + PQ  +R+PY++        +  +   P   L+  N+ +D++YY+   L  PL R+F  I      + +E+   K        +K +  + VG  +        C  C   L      IC+     +L   C E +   +L            +  L T C+ CS            H    C++ DCP+FY+R K ++ L     + ++E +  LN
Sbjct:  670 LTLEIHANTRSDKIPDPAIDEV----SMIIWCLEEETFPLDLDIAYEGIMIVHKASEDSTFPTKIQHCINEIPVMFYESEFEMFEALTDLVLLLDPDILSGFEIHNFSWGYIIERCQKIH--QFDIVRELARVKCQIKTKLSDTWGYAHSSG------IMITGRHMINIWRALRSDVNLTQYTIESAAFNILHKRLPHFSFESLTNMWNAKKSTTELKTVLNYWLSRAQINIQLLRKQDYIARNIEQARLIGIDFHSVYYRGSQFKVESFLIRICKSESFILLSPGKKDVRKQKALECVPLVMEPESAFYKSPLIVLDFQSLYPSIMIGYNYCYSTMIGR---VREINLTENNLGVSKFSLPRNILALLKNDVTIAPNGVVYAKTSVRKSTLSKMLTDILDVRVMIKKTMNEIGDDNTTLKR-LLNNKQLALKLLANVTYGYTSASFSGRMPCSDLADSIVQTGRETLEKAIDIIEKD------ETWNAKVVYGDTDSLFVYLPGKTAIEAFSIGHAMAERVTQNNPKPIFLKFEKVYHPSILISKKRYVGFSYESPSQTLPIFDAKGIETVRRDGIPAQQKIIEKCIRLLFQTKDLSKIKKYLQNEFFKIQIGKVSAQDFCFAKEVKLG--AYKSEKTAPAGAVVVKRRINEDHRAEPQYKERIPYLVVKGKQGQLLRERCVSPEEFLEGENLELDSEYYINKILIPPLDRLFNLIGINVGNWAQEIVKSK--RASTTTTKVENITRVGTSA-------TCCNCGEEL----TKICSL----QLCDDCLEKRSTTTLSFLIKKLKRQKEYQTLKTVCRTCSYRYTSDAGIENDHIASKCNSYDCPVFYSRVKAERYLRDNQSVQREEALISLN 1502          
BLAST of DNA polymerase vs. Ensembl Yeast
Match: POL1 (Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis [Source:SGD;Acc:S000005046])

HSP 1 Score: 161.384 bits (407), Expect = 1.782e-40
Identity = 163/644 (25.31%), Postives = 295/644 (45.81%), Query Frame = 3
Query: 1302 DPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHM------PGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKE----DVAHQIITDLQNGDDRTRHRLAVY-----CLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQS------------KDGD--------EFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDK-------MDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLL----CNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDP-------------IYVLDNNIGIDTDYYLKNQLTNPLLRIFEPI 3056
            DPD+I G+  QN  L  L +R + LN+  FS +GR   +R      ++  ++ GR  +   H        GR+  D+   + +    +  S +    + +  +KE    D+ +Q   + Q  +D     +A+      C+  +++  R+  +L+ +    ++  + G   +  L   +  +    LL + +   F++PD +             ++ D        ++ G  V EP KG +   +  +DF SLYPSI+   N+C+TT+ ++KE I       D+    P       S V +G+LP +L  L++ R++ K+ +  ETDP KR   D RQ ALK++ANS+YG  G    R     ++  VT  GRE++  T+++ ES          +  V+YGDTDSVM   G     +A+ +G    + ++ ++   ++++ + V+   LL +KK+YA +      + DK       ++ KG++  RR+ CPL  ++    L  +L  +D   AL      + D+      N I I +  I+ +L+K  + Y   +    +   ++ R  G   + G  + +VIT    +D+I   A+ P             + +  NN+  D  YYL+ Q+  P+ R+ E I
Sbjct:  631 DPDVIIGHRLQNVYLDVLAHRMHDLNIPTFSSIGR--RLR------RTWPEKFGRGNSNMNHFFISDICSGRLICDIANEMGQSLTPKCQSWDLSEMYQVTCEKEHKPLDIDYQ---NPQYQNDVNSMTMALQENITNCMISAEVSYRI--QLLTLTK--QLTNLAGNAWAQTLGGTRAGRNEYILLHEFSRNGFIVPDKEGNRSRAQKQRQNEENADAPVNSKKAKYQGGLVFEPEKGLHKNYVLVMDFNSLYPSIIQEFNICFTTVDRNKEDI-------DELPSVP------PSEVDQGVLPRLLANLVDRRREVKKVMKTETDPHKRVQCDIRQQALKLTANSMYGCLGYVNSRFYAKPLAMLVTNKGREILMNTRQLAES---------MNLLVVYGDTDSVMIDTGCDNYADAIKIGLGFKRLVNERYRL-LEIDIDNVFKKLLLHAKKKYAALTV----NLDKNGNGTTVLEVKGLDMKRREFCPLSRDVSIHVLNTILSDKDPEEALQEVYDYLEDIRIKVETNNIRIDKYKINMKLSKDPKAYPGGKNMPAVQVALRMRKAGRVVKAGSVITFVITK---QDEIDNAADTPALSVAERAHALNEVMIKSNNLIPDPQYYLEKQIFAPVERLLERI 1229          
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO49294 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7RH52])

HSP 1 Score: 1187.17 bits (3070), Expect = 0.000e+0
Identity = 572/920 (62.17%), Postives = 708/920 (76.96%), Query Frame = 3
Query:  729 IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVR----------PQTEKISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 3455
            IYG+ +N K  FLKVTV +  L+A  KR+L  G    HY     ++++++ID+ VRFMVDS + GC+WIELP   Y VR             +  S CQLEVDV WD++I H  E +W  IAP+RILSFDIECAGRKG FP+A+ DPVIQI+N+++R GE +PFI+N+FTL  CAPIVG+HV+    EA+L + WS F++E DPDIITGYN  NFDLPYL+NRA  L+V  FSLLGRI + +S +T +  QSK  G+R NK +++ GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H II+DLQNG+D+TR RLA+YC+KD+ L LRL+ KLM V+NY+EMARVTGVP+ YLLSRGQQIKV+SQLLRK    D V+P ++++ G+E+ GATVIEP KG+Y+ PIATLDF+SLYPSIMMAHNLCYT+LL   + ++E     D YIKTP G++FVK SV+KGLLP IL  L+ ARKKAK +L  ETDPFK++VLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVTAFGR MI+ TKE+VE+ +  ANGY  DAKVIYGDTDSVM  FGV TV E+M LG EAA+ +S  F+ PIKLEFEKVY+PYLLISKKRYAG+Y+TKPD  DKMDCKG+ETVRRDNCPLVANL+N CL+KLLI R+   A+ + + VI+DLLCNRIDISQL+I+KELTKT EEY  KQ HSELA++++KRD GSAP+LGDRVPYVI       K Y KAEDPIYVL+NN+ IDT YYL+NQL+ PLLRIFEPI G+  A+  LL GEH  +K    S VG LS +T  +  CIGCK +L DD  ++C HCK  E  ++ +E ++ + LE RF+RLWT+CQ C+GSLHQ+VLC++RDCPIFY R KVQK+L +QD+ + R   D  W
Sbjct:  177 IYGYHKNTKIPFLKVTVALQKLIAPAKRILETGFRCGHYPERGYSTYESNIDYEVRFMVDSDIVGCNWIELPAGKYRVRTPNGIASRYRSSAKPESRCQLEVDVAWDELISHEPEGDWSKIAPVRILSFDIECAGRKGIFPEAEKDPVIQIANMVIRQGEKDPFIRNVFTLGGCAPIVGSHVLSFDKEADLFKAWSEFVQECDPDIITGYNIVNFDLPYLLNRATALHVNNFSLLGRIVADKSTVTSTTFQSKAYGKRENKVINISGRVQFDLLHVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHNIISDLQNGNDQTRRRLAIYCMKDAILPLRLLEKLMCVINYMEMARVTGVPMGYLLSRGQQIKVMSQLLRKTHQTDLVIPAHKAEAGEEYAGATVIEPNKGYYNIPIATLDFSSLYPSIMMAHNLCYTSLLHPSQ-VKE--TPPDQYIKTPSGNFFVKKSVRKGLLPEILEHLLTARKKAKADLKKETDPFKKKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTAFGRMMIERTKELVEAKYTVANGYKTDAKVIYGDTDSVMVRFGVETVAESMELGNEAAEYVSEHFVKPIKLEFEKVYFPYLLISKKRYAGLYFTKPDKHDKMDCKGIETVRRDNCPLVANLINACLKKLLIERNPQGAVEYTKQVISDLLCNRIDISQLVITKELTKTGEEYAGKQAHSELAERMRKRDAGSAPKLGDRVPYVIIAGAKGAKAYEKAEDPIYVLENNVPIDTGYYLENQLSKPLLRIFEPILGESKASSALLKGEHTRTKTIVTSTVGKLSMFTKKRSTCIGCKALLDDDGSAVCKHCKSRESELFQKEIEHLRVLEERFARLWTQCQNCTGSLHQDVLCTSRDCPIFYMRTKVQKDLANQDKQVSRFG-DCFW 1092          

HSP 2 Score: 79.337 bits (194), Expect = 6.340e-15
Identity = 49/176 (27.84%), Postives = 76/176 (43.18%), Query Frame = 2
Query:   47 NSFQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNHEPEEICFEMESNTETVKETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNY--------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQ-TLKQRASQHQYAENLICKVEYCLKAS 547
            +S QK K    +   SK     +D D      +  E   +  + +   ET        KW R  +   +P    + FQQLD++NY           +  P  + R +G   +GNS++ H+HGF PY +V  PPGF + HC  FR  L++  L    S        +  V+Y ++ S
Sbjct:    2 DSSQKRKGAFGTGNASKRSRNQDDLDGSQPSSFESELASLQGD-DGAVETATRGRPSDKWPRPALPPFEPASDNIQFQQLDVENYIGTPLLGMPGSKVGPVPIIRMFGVTMDGNSVLCHIHGFVPYFFVPAPPGFQEIHCSLFRDTLDKAVLNDMRSNRDGVTQAVLAVDYMMRES 176          
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO42056 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S2J9])

HSP 1 Score: 243.047 bits (619), Expect = 4.393e-70
Identity = 163/497 (32.80%), Postives = 251/497 (50.50%), Query Frame = 3
Query: 1251 KTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGD-DRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVL--PDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIRE-----FGLT------------EDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETD-PFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANL 2666
            K E +L        RE DPD++ GY  Q     YLI RA   ++   S L R+    S  +   S+  Q G      + + GRI  +++ ++  +  L  YS   V+FH L E+     ++ ++D  +   D  R R   + +      +RL+ +L  +    E+AR+ G+    ++SRG Q +V S +LR A   +++   P  Q ++          V+EP   FY  P+  LDF SLYPS+M+A+N CY+T L    SI E     FG              E D   +P G  FVKS+V++G+LP +L  ++  R   K  +    D     ++LD RQL LK+ AN  YG+T A   GR+PC+ I+ S+    RE ++   ++V ++          A+V+YGDTDS+  L    +  EA  +G E  ++++ +   P+KL+FEKVY P +L +KKRY G  Y   D  + + D KG+ETVRRD CP VA +
Sbjct:    4 KKEKDLFPALLGLFREWDPDVLIGYEVQMLSWGYLIQRALVFDLDLASKLARVSGS-SSSSNMDSEKDQWGAAHASEIKIAGRIVLNVWRLMRHEVTLNVYSFENVAFHVLHERIPLFPYRSLSDWWDHKTDMHRWRTVQHYVTRCCGNVRLLEQLDFIGRTSELARLFGILFYSVISRGSQFRVESMMLRIAKPMNYIAASPSVQQRNSMRAPEVIPLVLEPESRFYHHPVLVLDFQSLYPSMMIAYNYCYSTCLGRVSSIAEGSHFKFGALSLSEPPSLLKQLERDIHISPNGVAFVKSNVRRGVLPRMLEDILNTRIMVKNSMKKWKDNKSTYRMLDARQLGLKLIANVTYGYTSANFSGRMPCVEIADSIVRKARETLERAIDLVNTTSKWG------ARVVYGDTDSLFVLLKGVSKDEAFRIGHEITETVTAQNPKPVKLKFEKVYLPCVLQTKKRYVGYMYETADQKEAVFDAKGIETVRRDTCPAVAKV 493          
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO39342 (DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7SAB6])

HSP 1 Score: 183.726 bits (465), Expect = 6.119e-47
Identity = 168/599 (28.05%), Postives = 289/599 (48.25%), Query Frame = 3
Query: 1236 HVIPHK-------TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPD--YQSK-----DGDE-------------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKREL-AIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSF---DKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQ----SVIADLLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKI--KKRDPGSAPQLGDRVPYVI 2915
            H IP         +E  LL      I +IDPD++ G++   FDL  L++R +   +  +S +GR+K  RS + + +    + G  ++  V   GR+  D+ +      + +SY L+ +S   L+  ++++    I ++ N      H L +  + DS L LR+M +L V+    ++  + G  LS  L  G+  +    LL     + ++ PD  Y  K     D D+             + G  V+EP KGFYDK I  LDF SLYPSI+  +N+C+TT+   ++S ++    EDD +  P            G+LP  + +L+E R++ K  +  +  D  K    D RQ ALK++ANS+YG  G    R     +++ VT+ GRE++  TK++V+              VIYGDTDS+M     + + E   +G +     +   + P+      V   ++L S  RYA +  ++ +     +  + KG++ VRRD C L  +  N  L ++L ++ + A + +      ++  D+   ++D+ + +I+K+LTK   +Y  K+  PH  +A  +  K R  G    +GD +PYVI
Sbjct:  393 HCIPQHLKIDVMASERALLAFVVAKIHKIDPDVLVGHDIFGFDLDILLHRISACKIPQWSRIGRLK--RSIMPKVRLSLGRGGHNVDPTVTC-GRLLCDVKISSRELIRSKSYDLSELSRVVLQTPRQELDQDDIGNMYNTSQELLHLLELTTV-DSLLTLRIMYELNVLPLAHQITTIAGNVLSRTLLGGRSKRNEFLLLHAFHEKGYICPDKSYGKKQVVREDDDDHVTTTKKGRRKPAYAGGLVLEPKKGFYDKFILLLDFNSLYPSIIQEYNICFTTI--DRKSAKD---EEDDIVDLP------DMDQDPGILPTEIRKLVERRRQVKGMMKTVSRDSDKYLQYDIRQKALKLTANSMYGCLGFSHSRFYAKPLAALVTSKGREILMKTKDLVQQMH---------LDVIYGDTDSIMINTNSTDLQEVKKVGNKVRHPHATYLVLPVSYTLRFVNLLHILFS--RYAAIMISENEDGTMNEVKEMKGLDIVRRDWCDLAKDAGNYVLSQILSAQSREAIVDNVHDFLITIGEDVKGGKVDLQKYVINKQLTKAPHDYPDKKSLPHVHVALWLMSKSRKVG----VGDTIPYVI 961          
BLAST of DNA polymerase vs. Ensembl Nematostella
Match: EDO27819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T875])

HSP 1 Score: 68.5514 bits (166), Expect = 1.673e-12
Identity = 54/193 (27.98%), Postives = 96/193 (49.74%), Query Frame = 3
Query: 2535 YPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQ----SVIADLLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKI--KKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL-----DNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELA 3074
            Y  ++IS+     M   K       + KG++ VRRD C L  +  N  L ++L ++ + A + +      ++  D+   ++D+ + +I+K+LTK   +Y  K+  PH  +A  +  K R  G    +GD +PYVI  + +   + T      Y+L      +++ IDT YYL NQ+   + R+ +PI G + A
Sbjct:   31 YAAIMISENEDGTMNEVK-------EMKGLDIVRRDWCDLAKDAGNYVLSQILSAQSREAIVDNVHDFLITIGEDVKGGKVDLQKYVINKQLTKAPHDYPDKKSLPHVHVALWLMSKSRKVG----VGDTIPYVICDVSMHLPLVTCGALVHYLLHEFRKSDSLKIDTKYYLANQVHPVVARLCDPIDGTDSA 212          
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pold1 (DNA polymerase delta 1, catalytic subunit [Source:NCBI gene;Acc:101155199])

HSP 1 Score: 1154.81 bits (2986), Expect = 0.000e+0
Identity = 556/919 (60.50%), Postives = 694/919 (75.52%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE------------KISFCQLEVDVQWDKIICHSTE-EWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYY-TKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLN 3440
            ++YG+       FL++T+ +P L+A  KR+L +G    H+      SF+ +IDF +RFMVDS + GC WIELP + + +R +              K+S CQ EVDV W  +I H  E EW  IAP+R+LSFDIECAGRKG FP+ D DPVIQI++++ R GE+EPFI+ +FTL +C  IVG+ ++    E +LL+ W+ F+R +DPDIITGYN QNFD PYLINRA  L V  F  LGR+++M+S + ES  QSKQMGRR NK ++M GR+ FDL  +LLRD+KLRSY+LNAVSFHFL+EQKEDV H II+DLQNG+++TR RLAVYCLKD+ L LRL+ KLM V+NY+EMARVTGVPLSYLLSRGQQIKV+SQLLR+A  ++ V+P  +++ G+++TGATVIEP KG+Y  PI TLDF+SLYPSIMMAHNLCYTTLL+ K S+    L+ +D+IKTP G  FVKSSV+KGLLP IL  L+ ARK+AK EL  ETDPFK+QVLDGRQLALKISANSVYGFTGAQ G+LPCL IS SVT FGR+MI+ TK++VES +  +NGY  DAKVIYGDTDSVM   GV+TV EAM +G+EAA+ +S  F  PIKLEFEKVYYPYLLI+KKRYAG+Y+ +  D+ DKMDCKG+ETVRRDNCPLVANL+NTCL+K+LI RD   A+ HA+ VI+DLLCNRIDISQL+I+KELT+T +EY  KQ H ELA++++KRD GSAP LGDRVPYVI         Y K+EDPIYVL+N I IDT YYL+ QL+ PLLRIFEPI G+  A   LL G+H   K    S VG L  +   +  CIGCK VL+ DE ++C  CK  E  +Y +E  +  +LE RFSRLWT+CQRC GSLH++VLC++RDCPIFY RKKVQK+L  Q +++ R  
Sbjct:  190 NMYGYHGQQSMDFLRITMAMPRLIAPAKRLLEQGFKFGHFPIQGYQSFEANIDFEIRFMVDSNVVGCCWIELPKDKWRLREERSTSTGEVDSQTPVKVSLCQYEVDVAWTDLISHPAEGEWQRIAPLRVLSFDIECAGRKGIFPEPDKDPVIQIASMVQRQGETEPFIRTVFTLQSCTSIVGSQILCFTQEKQLLQSWAEFLRTVDPDIITGYNIQNFDFPYLINRAAALKVTYFPYLGRVRNMKSVLKESNFQSKQMGRRENKTINMEGRVQFDLLQVLLRDYKLRSYTLNAVSFHFLQEQKEDVQHSIISDLQNGNEQTRRRLAVYCLKDAYLPLRLLQKLMCVINYMEMARVTGVPLSYLLSRGQQIKVVSQLLRQAMKQNLVMPVVKTEGGEDYTGATVIEPEKGYYSVPITTLDFSSLYPSIMMAHNLCYTTLLQ-KGSVERLQLSSEDFIKTPTGDQFVKSSVRKGLLPEILENLLSARKRAKAELKKETDPFKKQVLDGRQLALKISANSVYGFTGAQVGKLPCLEISQSVTGFGRQMIERTKQLVESKYTISNGYQADAKVIYGDTDSVMVKLGVATVKEAMEIGREAAEWVSSHFTPPIKLEFEKVYYPYLLINKKRYAGLYFSSSADTHDKMDCKGIETVRRDNCPLVANLINTCLQKILIDRDPQGAVDHAKEVISDLLCNRIDISQLVITKELTRTAQEYAGKQAHVELAERMRKRDAGSAPNLGDRVPYVIIKAAKGAAAYMKSEDPIYVLENTIPIDTQYYLEQQLSKPLLRIFEPILGESKAESVLLKGDHTRCKTVLTSKVGGLMAFAQKRSTCIGCKAVLKTDE-AVCDFCKKKESELYQKEIFHLNTLEERFSRLWTQCQRCQGSLHEDVLCTSRDCPIFYMRKKVQKDLDDQSKLVSRFG 1106          

HSP 2 Score: 75.8702 bits (185), Expect = 2.159e-13
Identity = 36/95 (37.89%), Postives = 52/95 (54.74%), Query Frame = 2
Query:  221 KWARQNMDVVKPEIMPLIFQQLDIDNY----------EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTL 475
            +W R +   + P    L+FQQ+D+D+Y          +++E  P  + R +G   NGNS+  HVHGF PY YV  P GF  E  GEF++ LN  +
Sbjct:   73 RWPRPHAPTLNPSSETLVFQQIDLDHYLGASVAGMPGQLQEKVP--IIRMFGVTDNGNSVCCHVHGFAPYFYVPAPNGFKSEFLGEFKRELNSAV 165          
BLAST of DNA polymerase vs. Ensembl Medaka
Match: rev3l (REV3 like, DNA directed polymerase zeta catalytic subunit [Source:NCBI gene;Acc:101170069])

HSP 1 Score: 291.967 bits (746), Expect = 2.547e-80
Identity = 236/833 (28.33%), Postives = 391/833 (46.94%), Query Frame = 3
Query: 1050 PSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKN---IFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITD-LQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDF--VLPDYQSKDGDEFTGAT--VIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIR-----EFGLT------------EDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAI-ETDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKM-DCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSK-QPHSELAQKIKKRDPGSAPQLGDRVPYVIT--TLGVKDKIYTKAEDPIYVL-DNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRD----------DEKS---ICAHCK--PNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKEL 3407
            P   PI  L +   C       PDAD     Q++  I+   + EP  +       L   + + G  V     E  L +     +R +DPDI+ GY  Q     YL+ RA  L V     L R+    SK     ++  + G      +H+ GR+  +L+ ++  +  L SYS   V+FH L ++    +H+ ++D   +  +  R ++  + +      + L+ +  +V    E+AR+ G+   ++L+RG Q +V S +LR A   ++  V P  Q +           V+EP   FY   +  LDF SLYPSI++A+N C++T L  +ES+      +FG T             +D   +P G  FVKS+V+KG+LP +L +++  R   K+ +   + D    ++LD RQL LK+ AN  +G+T A   GR+P + +  S+    RE ++   + V  +  K  G    A+V+YGDTDS+  L   +T  +A  +G E A++++     P+KL+FEKVY P +L +KKRY G  Y   D  + + D KG+ETVRRD CP V+ +L   ++ L  +RD +      Q     +L  R  +  L  +KE   +         P  EL +++   D    P++G+RVPYV+   T GV   +      P+ VL D  + ++  YY+  Q+  PL R+F+            L G  V S +++   V   S         +G K  +            DE +   +C+ C+  P  +A+   +    +  E +  +L   C+ CSG   ++V C + DCP+ Y   +V +++
Sbjct: 2151 PEFDPICALFY---CFSSDAPLPDAD---STQLTGAIMVDKDHEPGGRGRRETAPLLVRSGVSGLQVTYASEEKMLFQELVGIMRRLDPDILLGYEVQMHSWGYLLQRAAVLGVDLCQQLSRVPG-DSKENRFAAEKDEYGADTMTEIHIIGRVTLNLWRVMKSEVALNSYSFENVAFHVLHQRFPLFSHRTLSDWFDHNTNLYRWKMVDHYVSRVCSSMHLLQQNDIVGRTSELARLFGIQFLHVLTRGSQYRVESMMLRVAKPLNYIPVTPSVQQRAQQRAPQCIPLVMEPESRFYSNSVLVLDFQSLYPSIVIAYNYCFSTCLGHQESLGTPDEFKFGCTSLRVPPEMLYQLRNDINVSPSGIAFVKSTVRKGVLPRMLEEILNTRIMVKQSMKTYKQDKALMKLLDARQLGLKLIANVTFGYTSANYSGRMPSVEVGDSIVHKARETLERAIKQVNDT--KKWG----ARVVYGDTDSMFVLLKGATKEQAFRIGNEIAEAVTATNPKPVKLKFEKVYLPCVLQTKKRYVGYMYESLDQKEPVFDAKGIETVRRDGCPAVSKILERSIKLLFETRDISQVKQFVQCQCLKVLDGRASMQDLTFAKEYRGSGSYRPGACVPALELTRRMLSYDRRLEPRVGERVPYVVVYGTPGV--PLIQLVRRPLDVLQDPTLRLNATYYITKQILPPLARMFQ------------LIGVDVFSWYQELPRVQKASCSAAAAGDEVGRKGTISQYFTTLHCPVCDELTQLGVCSQCRAEPQRVAVTLYQDM--RQWESQQEQLLKICRSCSGGTERQVPCISLDCPVLYKLSRVNRQI 2954          
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pola1 (DNA polymerase alpha 1, catalytic subunit [Source:NCBI gene;Acc:101165771])

HSP 1 Score: 180.644 bits (457), Expect = 1.646e-45
Identity = 171/637 (26.84%), Postives = 305/637 (47.88%), Query Frame = 3
Query: 1254 TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRR---INKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQS---------------KDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLK-SKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIE-TDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGM---YYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIAD----LLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDN-----NIGIDTDYYLKNQLTNPLLRIFEPIFG 3062
            TE  LL  +   + +IDPD++ G++   FDL  L+ R N   V  +S +GR++         ++   ++G R     KN    GR+  D+ +      + +SY L  ++   L+ ++  V  + + +L +      + L +    D+ L+L++M +L V+   +++  + G  LS  L  G+  +    LL     +++++PD  S               +    ++G  V++P  GFYD+ +  LDF SLYPSI+   N+C+TT+ + ++ S R+  + E   I  P         ++ G+LP  + +L+E RK+ K+ +  +  +P      D RQ ALK++ANS+YG  G    R     +++ VT  GRE++  TK+MV+           + +VIYGDTDS+M      ++ E   LG +    ++ K    ++++ + V+   LL+ KK+YA +   ++       K + KG++ VRRD C L     N  + ++L  + ++  + + Q  + +    +    I ++   I K LTK  ++Y  K+  PH  +A  I  +  G   + GD + Y+I     KD     A    Y L+       + +DT YYL  Q+   + RI EPI G
Sbjct:  610 TERTLLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLR---------RANMPKLGGRGLFAEKNATC-GRLVCDVEISAKELIRCKSYHLTELAAQVLKVERVTVPQEEVKNLYSDSPHLLYLLEL-TWTDAKLILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFHDKNYIVPDKPSFKKAQLEMVGTFKKTRKKAAYSGGLVLDPKVGFYDQFVLLLDFNSLYPSIIQEFNICFTTVQREAQNSRRKREVEEIPEIPDP--------GLEMGILPKEIRKLVERRKQVKQLMKQQDINPDLYMQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKDMVQKM---------NLEVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEVN-KLYKLLEIDIDGVFKSLLLLKKKKYAALVVEHHGDGRYSVKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDIIVENIQKHLVEVGERVANGDIPLNLYEIHKALTKDPQDYPDKKSLPHVHVALWINSQG-GRRVKAGDTISYLI----CKDGSTLAASQRAYALEQLQKQEGLSVDTQYYLAQQIHPVVSRICEPIEG 1212          
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pola1 (DNA polymerase alpha 1, catalytic subunit [Source:NCBI gene;Acc:101165771])

HSP 1 Score: 177.563 bits (449), Expect = 1.377e-44
Identity = 170/643 (26.44%), Postives = 306/643 (47.59%), Query Frame = 3
Query: 1254 TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRR---INKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQS--------KDGDE--------------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIE-TDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGM---YYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIAD----LLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDN-----NIGIDTDYYLKNQLTNPLLRIFEPIFG 3062
            TE  LL  +   + +IDPD++ G++   FDL  L+ R N   V  +S +GR++         ++   ++G R     KN    GR+  D+ +      + +SY L  ++   L+ ++  V  + + +L +      + L +    D+ L+L++M +L V+   +++  + G  LS  L  G+  +    LL     +++++PD  S         DG+E              ++G  V++P  GFYD+ +  LDF SLYPSI+   N+C+TT+ +  ++ R     + +  ++          ++ G+LP  + +L+E RK+ K+ +  +  +P      D RQ ALK++ANS+YG  G    R     +++ VT  GRE++  TK+MV+           + +VIYGDTDS+M      ++ E   LG +    ++ K    ++++ + V+   LL+ KK+YA +   ++       K + KG++ VRRD C L     N  + ++L  + ++  + + Q  + +    +    I ++   I K LTK  ++Y  K+  PH  +A  I  +  G   + GD + Y+I     KD     A    Y L+       + +DT YYL  Q+   + RI EPI G
Sbjct:  613 TERTLLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLR---------RANMPKLGGRGLFAEKNATC-GRLVCDVEISAKELIRCKSYHLTELAAQVLKVERVTVPQEEVKNLYSDSPHLLYLLEL-TWTDAKLILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFHDKNYIVPDKPSFKKAQLEMGDGEEDVDAGKGKRKKKAAYSGGLVLDPKVGFYDQFVLLLDFNSLYPSIIQEFNICFTTVQREAQNSRR----KREEDESEEIPEIPDPGLEMGILPKEIRKLVERRKQVKQLMKQQDINPDLYMQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKDMVQKM---------NLEVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEVN-KLYKLLEIDIDGVFKSLLLLKKKKYAALVVEHHGDGRYSVKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDIIVENIQKHLVEVGERVANGDIPLNLYEIHKALTKDPQDYPDKKSLPHVHVALWINSQG-GRRVKAGDTISYLI----CKDGSTLAASQRAYALEQLQKQEGLSVDTQYYLAQQIHPVVSRICEPIEG 1225          
BLAST of DNA polymerase vs. Ensembl Medaka
Match: pola1 (DNA polymerase alpha 1, catalytic subunit [Source:NCBI gene;Acc:101165771])

HSP 1 Score: 177.178 bits (448), Expect = 2.158e-44
Identity = 170/643 (26.44%), Postives = 306/643 (47.59%), Query Frame = 3
Query: 1254 TEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRR---INKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQS--------KDGDE--------------FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIE-TDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGM---YYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIAD----LLCNRIDISQLIISKELTKTDEEYTSKQ--PHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDN-----NIGIDTDYYLKNQLTNPLLRIFEPIFG 3062
            TE  LL  +   + +IDPD++ G++   FDL  L+ R N   V  +S +GR++         ++   ++G R     KN    GR+  D+ +      + +SY L  ++   L+ ++  V  + + +L +      + L +    D+ L+L++M +L V+   +++  + G  LS  L  G+  +    LL     +++++PD  S         DG+E              ++G  V++P  GFYD+ +  LDF SLYPSI+   N+C+TT+ +  ++ R     + +  ++          ++ G+LP  + +L+E RK+ K+ +  +  +P      D RQ ALK++ANS+YG  G    R     +++ VT  GRE++  TK+MV+           + +VIYGDTDS+M      ++ E   LG +    ++ K    ++++ + V+   LL+ KK+YA +   ++       K + KG++ VRRD C L     N  + ++L  + ++  + + Q  + +    +    I ++   I K LTK  ++Y  K+  PH  +A  I  +  G   + GD + Y+I     KD     A    Y L+       + +DT YYL  Q+   + RI EPI G
Sbjct:  617 TERTLLGFFLAKMHKIDPDVLVGHDIFGFDLEVLLQRINVCKVPHWSKIGRLR---------RANMPKLGGRGLFAEKNATC-GRLVCDVEISAKELIRCKSYHLTELAAQVLKVERVTVPQEEVKNLYSDSPHLLYLLEL-TWTDAKLILQIMCELNVLPLALQITNIAGNVLSRTLMGGRSERNEYLLLHAFHDKNYIVPDKPSFKKAQLEMGDGEEDVDAGKGKRKKKAAYSGGLVLDPKVGFYDQFVLLLDFNSLYPSIIQEFNICFTTVQREAQNSRR----KREEDESEEIPEIPDPGLEMGILPKEIRKLVERRKQVKQLMKQQDINPDLYMQYDIRQKALKLTANSMYGCLGFSFSRFYAKPLAALVTHKGREILMHTKDMVQKM---------NLEVIYGDTDSIMINTNSKSLEEVFKLGNKVKAEVN-KLYKLLEIDIDGVFKSLLLLKKKKYAALVVEHHGDGRYSVKQELKGLDIVRRDWCDLAKECGNYVIGQILSDQSRDIIVENIQKHLVEVGERVANGDIPLNLYEIHKALTKDPQDYPDKKSLPHVHVALWINSQG-GRRVKAGDTISYLI----CKDGSTLAASQRAYALEQLQKQEGLSVDTQYYLAQQIHPVVSRICEPIEG 1229          
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000033372.1 (SMESG000033372.1)

HSP 1 Score: 1836.23 bits (4755), Expect = 0.000e+0
Identity = 891/910 (97.91%), Postives = 892/910 (98.02%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTEEWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 3455
            +IYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYT                  WDKIICHSTEEWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW
Sbjct:  172 NIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYT------------------WDKIICHSTEEWPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMIDFTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 1063          

HSP 2 Score: 367.466 bits (942), Expect = 5.813e-109
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 2
Query:   32 MSRRPNSFQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNHEPEEICFEMESNTETVKETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNYEIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRASQHQYAENLICKVEYCLKA 544
            MSRRPNSFQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNHEPEEICFEMESNTETVKETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNYEIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRASQHQYAENLICKVEYCLKA
Sbjct:    1 MSRRPNSFQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNHEPEEICFEMESNTETVKETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNYEIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRASQHQYAENLICKVEYCLKA 171          
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000033513.1 (SMESG000033513.1)

HSP 1 Score: 639.032 bits (1647), Expect = 0.000e+0
Identity = 319/499 (63.93%), Postives = 396/499 (79.36%), Query Frame = 3
Query:  726 DIYGFSQNLKKAFLKVTVPVPSLVATTKRVLVEGMTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTEKISFCQLEVDVQWDKIICHSTEE-WPSIAPIRILSFDIECAGRKGSFPDADIDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIREFGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETDPFKRQVLDGRQLAL 2219
            +IYG+SQ+ K  F+K+T+ +PSL++T KR+L EG+ IP+ G+HNL++FD +IDF++RFMVDSG  GCSWIELP N+Y +R +  K+S CQLEVDV W  I  H   + W  I PIRI+SFD+ C   KGS P+ + DPVIQISN ++RFGE EPFI+ IFTLN+C P+VGA V  H TE +LL+HWS FIREIDPD+I+GYNTQ  DLPYLINRA KLN+  F+LLGRIK  +S I  S  QSKQ GR+  K VHM GR+ FDL  I+ RD+KLRSYSL+AV+FHF EEQK+  +HQ+ITDLQNG + TRHRLAVY +K S L LRL+NKLM+V+NYVEMARV GVPLSYLL RGQ IKVLS +LRKA   DFVLPDY++   ++++G  +IEP +G+Y KPIA LDFASLYPSIM+AHNLCYTTLL+ K  I++FGL+E DY KTP  SYFVKSSVKKGLLP IL++L++ARKK K+E+A E+D F +QVLDGRQLA+
Sbjct:  167 NIYGYSQDKKFNFIKITLLLPSLLSTCKRLLEEGLIIPNCGFHNLSAFDINIDFTLRFMVDSGFVGCSWIELPTNSYQIRCEESKMSLCQLEVDVNWKLITYHPPNKYWSHIVPIRIISFDVMCFSTKGSSPNPETDPVIQISNTVMRFGEQEPFIQCIFTLNSCVPLVGAKVFQHDTEEDLLKHWSQFIREIDPDLISGYNTQTLDLPYLINRAKKLNIIHFNLLGRIKFAQSLIGISSLQSKQFGRKSYKIVHMAGRVDFDLKQIISRDYKLRSYSLHAVAFHFFEEQKQKFSHQVITDLQNGTEFTRHRLAVYSVKTSFLSLRLINKLMLVINYVEMARVAGVPLSYLLLRGQSIKVLSLILRKAKENDFVLPDYKANSSEQYSGGLIIEPERGYYTKPIAVLDFASLYPSIMIAHNLCYTTLLRDKNIIKQFGLSESDYSKTPIESYFVKSSVKKGLLPVILLELLDARKKTKQEMACESDLFMKQVLDGRQLAI 665          

HSP 2 Score: 115.546 bits (288), Expect = 5.146e-26
Identity = 56/124 (45.16%), Postives = 75/124 (60.48%), Query Frame = 2
Query:  173 EMESNTETVKETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNYEIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEFRKILNQTLKQRASQHQYAENLICKVEYCLKA 544
            +  S  E +  T+EF+ W R  +       MP+IFQQ+DID+Y I  +NP  VFR +G N++GNSI+A +HGFYPY YV+ P  F    C  FR  LN+ LK +  +H+   NLI  VE  LKA
Sbjct:   43 DFTSIPEDLYSTNEFNIWKRPRIIDFDSNTMPVIFQQIDIDHYVITGSNPEPVFRIFGINESGNSILALIHGFYPYFYVKSPENFKIGDCETFRDFLNELLKSKTKRHKEIGNLIINVELFLKA 166          
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000033512.1 (SMESG000033512.1)

HSP 1 Score: 513.842 bits (1322), Expect = 7.810e-173
Identity = 237/346 (68.50%), Postives = 293/346 (84.68%), Query Frame = 3
Query: 2418 MCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHCKPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIFYTRKKVQKELISQDEIMERLNQDLSW 3455
            MCL  V TV EA+ LGK+AA+ IS KFI+PIK+EFE VY PYL+++KKRYAG+ YTKPD +DKM+CKG+ETVRRD+CPLV NLLN+CL++LLI RDK +AL HAQSVIADL+CN+IDISQLIIS+EL KT++EYTSKQPHSELA K+ KRDP SAPQLGDRV YVITT G+++K YTKAEDP+YVL+N++ IDT+YYLKNQL NPLLRIFEPIFGK++ +R+LL+GEH+L+K K QSN G L KYTVIKPKC+ CKN+L+++EKS+C HC+ N   IY RE    +SLE +F RLWTECQRC+ +LH+EVLCSN DCPIF+ RK +QK LI Q+E +E+  +DL W
Sbjct:    1 MCLLNVETVSEAINLGKKAAEIISTKFISPIKMEFENVYLPYLIVNKKRYAGICYTKPDVYDKMNCKGIETVRRDSCPLVGNLLNSCLKRLLIDRDKESALQHAQSVIADLMCNKIDISQLIISRELNKTNKEYTSKQPHSELALKMFKRDPRSAPQLGDRVSYVITTHGIQNKAYTKAEDPLYVLENDLIIDTNYYLKNQLINPLLRIFEPIFGKDVTSRRLLYGEHMLTKSKPQSNTGPLFKYTVIKPKCVSCKNILQNNEKSLCLHCRDNASTIYLREITCLRSLEKKFCRLWTECQRCTENLHKEVLCSNHDCPIFFKRKAIQKSLIMQNETIEKFERDLEW 346          
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000077868.1 (SMESG000077868.1)

HSP 1 Score: 284.263 bits (726), Expect = 2.271e-78
Identity = 226/792 (28.54%), Postives = 374/792 (47.22%), Query Frame = 3
Query: 1236 HVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDL--QNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFV-----LPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLL---------KSKESIREFG----LTEDDYIK--------TPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIE--TDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVES------SFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFD-KMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELT-KTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL---------DNNIGIDTD--------YYLKNQLTNPLLR----IFEPIFG--KELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKS--------ICAHC-KPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSL--HQEV--LCSNRDCPIFYTR 3386
            H +   +E EL++     + E DPDI+ G+ T      YL+ R+  +N K +  L R+       T++ S    +      ++H  GR+  DL++++  +  L SY+L   +   L+E+     +Q +      N D  T      Y +  +   +R++ K+ ++    E AR+ G+   ++LSRG Q +V S +LR A  ++ +     +     +  +E    T +EP  G Y  P+  LDF SLYPSIM+ +N CY T L          S++   E G    + ++  I+        +P G  FV+  ++KG++  ++ +++  R   K+ + I     P  + +LD RQL LK+ AN  YG+T A   GR+PC+ +  S+    RE ++ T ++V S      S   + G     KVIYGDTDSV  +    T  EA  LG   A +++     P+KL+FEKV+ P LL +KKRY G+ Y  PD    K D KG+ETVRRDNC +V  +L   L +L  S D N+   +    +  ++   I+I   II+KE   +   +  S  P   +A K+ K+DP + P  G+RVPYVI      D + +    P  +L          NNI +  +        YY+  Q+  PL R    I + +F    ++  R   +G     +   +     +S Y      C+ C + ++ ++ S        +C +C +  +L IY    K  K +E     +  +C  C+ ++  H+ +   C ++DCPIF+ R
Sbjct:  704 HFVRVASETELMDKIIELVWEFDPDILVGFETVRSSWGYLLERSFNINHKLYHGLSRLIIQN---TDAFSTKCSINSYKTYDLHCTGRMVLDLWIVIKHEITLNSYTLENCADEILKERVPYYTNQQLMAFYRSNTDRDTLMNFFFYRVLAN---IRILTKMNLISQISEFARIYGIEFYHVLSRGSQYRVESMMLRLAKPQNLIAISPNVSQRAQQKANECIPLT-LEPKSGLYWDPVVVLDFQSLYPSIMIGYNYCYATTLGKLDHIINVDSRQESYELGCSRMMLDNSSIRKVGSGVSISPNGVAFVRPEIQKGIISKMVEEILNTRIMVKKSMKIHAANKPLCK-LLDARQLGLKLIANVTYGYTAANFSGRMPCVEVGDSIVRKSRETLEETIKLVNSGGPNHVSKLDSEGGWAGCKVIYGDTDSVFVVMPNRTKDEAFVLGARIADAVTSVNPFPVKLKFEKVFLPCLLEAKKRYVGLSYESPDQIQPKFDAKGIETVRRDNCGIVGKILERTLNRLF-SSDINSVKRYVTQQLDQIMRGDINIFDFIIAKEYRGRGGYKANSSVPAVHIANKLVKKDPRAEPLNGERVPYVIIQGAPGDTLISCVRQPEEILFNTKMLKAYGNNIRLLNEQGCKLSYTYYITKQILPPLNRALNLIGQDVFSWYSKMPKRLKSYGNGYRGQCSSKLLNRTISSY-FQSQSCLICNSTVKSNQNSDSNSFSSVLCENCLRKGKLHIYLTAMKQLKDIEAYRKSILDKCFLCTNTVPTHRGIGPPCVSKDCPIFFDR 1485          
BLAST of DNA polymerase vs. Planmine SMEST
Match: SMESG000077868.1 (SMESG000077868.1)

HSP 1 Score: 284.263 bits (726), Expect = 2.415e-78
Identity = 226/792 (28.54%), Postives = 374/792 (47.22%), Query Frame = 3
Query: 1236 HVIPHKTEAELLEHWSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSKITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFHFLEEQKEDVAHQIITDL--QNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVVNYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFV-----LPDYQSKDGDEFTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLL---------KSKESIREFG----LTEDDYIK--------TPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIE--TDPFKRQVLDGRQLALKISANSVYGFTGAQ-RGRLPCLNISSSVTAFGREMIDFTKEMVES------SFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAAQSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFD-KMDCKGMETVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQLIISKELT-KTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLGVKDKIYTKAEDPIYVL---------DNNIGIDTD--------YYLKNQLTNPLLR----IFEPIFG--KELATRKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKS--------ICAHC-KPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSL--HQEV--LCSNRDCPIFYTR 3386
            H +   +E EL++     + E DPDI+ G+ T      YL+ R+  +N K +  L R+       T++ S    +      ++H  GR+  DL++++  +  L SY+L   +   L+E+     +Q +      N D  T      Y +  +   +R++ K+ ++    E AR+ G+   ++LSRG Q +V S +LR A  ++ +     +     +  +E    T +EP  G Y  P+  LDF SLYPSIM+ +N CY T L          S++   E G    + ++  I+        +P G  FV+  ++KG++  ++ +++  R   K+ + I     P  + +LD RQL LK+ AN  YG+T A   GR+PC+ +  S+    RE ++ T ++V S      S   + G     KVIYGDTDSV  +    T  EA  LG   A +++     P+KL+FEKV+ P LL +KKRY G+ Y  PD    K D KG+ETVRRDNC +V  +L   L +L  S D N+   +    +  ++   I+I   II+KE   +   +  S  P   +A K+ K+DP + P  G+RVPYVI      D + +    P  +L          NNI +  +        YY+  Q+  PL R    I + +F    ++  R   +G     +   +     +S Y      C+ C + ++ ++ S        +C +C +  +L IY    K  K +E     +  +C  C+ ++  H+ +   C ++DCPIF+ R
Sbjct:  775 HFVRVASETELMDKIIELVWEFDPDILVGFETVRSSWGYLLERSFNINHKLYHGLSRLIIQN---TDAFSTKCSINSYKTYDLHCTGRMVLDLWIVIKHEITLNSYTLENCADEILKERVPYYTNQQLMAFYRSNTDRDTLMNFFFYRVLAN---IRILTKMNLISQISEFARIYGIEFYHVLSRGSQYRVESMMLRLAKPQNLIAISPNVSQRAQQKANECIPLT-LEPKSGLYWDPVVVLDFQSLYPSIMIGYNYCYATTLGKLDHIINVDSRQESYELGCSRMMLDNSSIRKVGSGVSISPNGVAFVRPEIQKGIISKMVEEILNTRIMVKKSMKIHAANKPLCK-LLDARQLGLKLIANVTYGYTAANFSGRMPCVEVGDSIVRKSRETLEETIKLVNSGGPNHVSKLDSEGGWAGCKVIYGDTDSVFVVMPNRTKDEAFVLGARIADAVTSVNPFPVKLKFEKVFLPCLLEAKKRYVGLSYESPDQIQPKFDAKGIETVRRDNCGIVGKILERTLNRLF-SSDINSVKRYVTQQLDQIMRGDINIFDFIIAKEYRGRGGYKANSSVPAVHIANKLVKKDPRAEPLNGERVPYVIIQGAPGDTLISCVRQPEEILFNTKMLKAYGNNIRLLNEQGCKLSYTYYITKQILPPLNRALNLIGQDVFSWYSKMPKRLKSYGNGYRGQCSSKLLNRTISSY-FQSQSCLICNSTVKSNQNSDSNSFSSVLCENCLRKGKLHIYLTAMKQLKDIEAYRKSILDKCFLCTNTVPTHRGIGPPCVSKDCPIFFDR 1556          
The following BLAST results are available for this feature:
BLAST of DNA polymerase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
POLD15.377e-1359.27DNA polymerase delta 1, catalytic subunit [Source:... [more]
POLD15.377e-1359.27DNA polymerase delta 1, catalytic subunit [Source:... [more]
POLD15.377e-1359.27DNA polymerase delta 1, catalytic subunit [Source:... [more]
POLD14.901e-1357.62DNA polymerase delta 1, catalytic subunit [Source:... [more]
POLD14.813e-1357.17DNA polymerase delta 1, catalytic subunit [Source:... [more]
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BLAST of DNA polymerase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
F10C2.40.000e+053.99DNA polymerase delta catalytic subunit [Source:Un... [more]
rev-32.283e-6128.37DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A0A0K... [more]
rev-36.827e-6128.37DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A0A0K... [more]
pola-12.323e-4626.80DNA polymerase [Source:UniProtKB/TrEMBL;Acc:C7IVS... [more]
pola-12.484e-4626.80DNA polymerase [Source:UniProtKB/TrEMBL;Acc:C7IVS... [more]
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BLAST of DNA polymerase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
DNApol-delta2.111e-1159.56gene:FBgn0263600 transcript:FBtr0075522[more]
DNApol-zeta6.757e-7729.89gene:FBgn0002891 transcript:FBtr0088831[more]
DNApol-alpha1803.335e-4223.75gene:FBgn0259113 transcript:FBtr0084115[more]
DNApol-epsilon2553.135e-631.58gene:FBgn0264326 transcript:FBtr0084408[more]
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BLAST of DNA polymerase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pold13.237e-1260.81polymerase (DNA directed), delta 1, catalytic subu... [more]
rev3l4.446e-8730.49REV3 like, DNA directed polymerase zeta catalytic ... [more]
rev3l1.020e-8630.49REV3 like, DNA directed polymerase zeta catalytic ... [more]
pola12.103e-4925.70polymerase (DNA directed), alpha 1 [Source:ZFIN;Ac... [more]
pola13.626e-4925.70polymerase (DNA directed), alpha 1 [Source:ZFIN;Ac... [more]
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BLAST of DNA polymerase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
plscr23.755e-1060.74phospholipid scramblase 2 [Source:Xenbase;Acc:XB-G... [more]
plscr23.448e-1060.74phospholipid scramblase 2 [Source:Xenbase;Acc:XB-G... [more]
plscr23.500e-1060.15phospholipid scramblase 2 [Source:Xenbase;Acc:XB-G... [more]
plscr22.887e-1059.21phospholipid scramblase 2 [Source:Xenbase;Acc:XB-G... [more]
plscr24.276e-1059.43phospholipid scramblase 2 [Source:Xenbase;Acc:XB-G... [more]
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BLAST of DNA polymerase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pold11.351e-1258.17polymerase (DNA directed), delta 1, catalytic subu... [more]
Pold11.013e-1260.20polymerase (DNA directed), delta 1, catalytic subu... [more]
Rev3l9.198e-8429.34REV3 like, DNA directed polymerase zeta catalytic ... [more]
Rev3l9.198e-8429.34REV3 like, DNA directed polymerase zeta catalytic ... [more]
Pola11.621e-4928.97polymerase (DNA directed), alpha 1 [Source:MGI Sym... [more]
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BLAST of DNA polymerase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P28339|DPOD1_BOVIN9.779e-1259.27DNA polymerase delta catalytic subunit OS=Bos taur... [more]
sp|P54358|DPOD1_DROME2.116e-1059.56DNA polymerase delta catalytic subunit OS=Drosophi... [more]
sp|P28340|DPOD1_HUMAN2.582e-1259.27DNA polymerase delta catalytic subunit OS=Homo sap... [more]
sp|P97283|DPOD1_MESAU5.752e-1158.28DNA polymerase delta catalytic subunit OS=Mesocric... [more]
sp|P52431|DPOD1_MOUSE9.449e-1258.17DNA polymerase delta catalytic subunit OS=Mus musc... [more]
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BLAST of DNA polymerase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1PLY13.528e-1364.00DNA polymerase OS=Crassostrea gigas OX=29159 GN=CG... [more]
R7TGI42.111e-962.98DNA polymerase OS=Capitella teleta OX=283909 GN=CA... [more]
A0A267EYR03.326e-963.47DNA polymerase (Fragment) OS=Macrostomum lignano O... [more]
A0A293LKP11.500e-1163.14DNA polymerase (Fragment) OS=Ornithodoros erraticu... [more]
A0A267EYT14.627e-963.47DNA polymerase (Fragment) OS=Macrostomum lignano O... [more]
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BLAST of DNA polymerase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pold11.338e-1359.76DNA polymerase delta 1, catalytic subunit [Source:... [more]
pold11.216e-1359.76DNA polymerase delta 1, catalytic subunit [Source:... [more]
pold12.733e-1457.02DNA polymerase delta 1, catalytic subunit [Source:... [more]
rev3l2.455e-8428.79REV3 like, DNA directed polymerase zeta catalytic ... [more]
rev3l4.958e-8328.76REV3 like, DNA directed polymerase zeta catalytic ... [more]
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BLAST of DNA polymerase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pold18.790e-1262.27polymerase (DNA directed), delta 1, catalytic subu... [more]
ENSPMAT00000009864.12.416e-4929.21pep scaffold:Pmarinus_7.0:GL480102:12343:24944:1 g... [more]
ENSPMAT00000006446.19.899e-2726.36pep scaffold:Pmarinus_7.0:GL477096:156518:193524:1... [more]
ENSPMAT00000002970.14.547e-620.29pep scaffold:Pmarinus_7.0:GL476977:35626:117313:1 ... [more]
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BLAST of DNA polymerase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
POL30.000e+052.41Catalytic subunit of DNA polymerase delta; require... [more]
REV31.518e-8026.89Catalytic subunit of DNA polymerase zeta; involved... [more]
POL11.782e-4025.31Catalytic subunit of the DNA polymerase I alpha-pr... [more]
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BLAST of DNA polymerase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO492946.340e-1562.17DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7RH5... [more]
EDO420564.393e-7032.80DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7S2J... [more]
EDO393426.119e-4728.05DNA polymerase [Source:UniProtKB/TrEMBL;Acc:A7SAB... [more]
EDO278191.673e-1227.98Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA polymerase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pold12.159e-1360.50DNA polymerase delta 1, catalytic subunit [Source:... [more]
rev3l2.547e-8028.33REV3 like, DNA directed polymerase zeta catalytic ... [more]
pola11.646e-4526.84DNA polymerase alpha 1, catalytic subunit [Source:... [more]
pola11.377e-4426.44DNA polymerase alpha 1, catalytic subunit [Source:... [more]
pola12.158e-4426.44DNA polymerase alpha 1, catalytic subunit [Source:... [more]
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BLAST of DNA polymerase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000033372.15.813e-10997.91SMESG000033372.1[more]
SMESG000033513.15.146e-2663.93SMESG000033513.1[more]
SMESG000033512.17.810e-17368.50SMESG000033512.1[more]
SMESG000077868.12.271e-7828.54SMESG000077868.1[more]
SMESG000077868.12.415e-7828.54SMESG000077868.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004407 ID=SMED30004407|Name=DNA polymerase|organism=Schmidtea mediterranea sexual|type=transcript|length=3559bp
TTTATTCAAGTCTAATCTTAAAAAAAGTAAGATGAGTCGTCGTCCTAATT
CATTTCAAAAAAATAAAACTTATGCACCTTCTTCTAAAATTTCCAAGTTT
GAAGTCAAGAATAATGATGAGGATGATGTTTCTATGGATGAATGGAATCA
TGAACCCGAGGAAATTTGTTTTGAAATGGAATCCAATACAGAAACAGTAA
AAGAAACTGATGAATTTTCCAAGTGGGCAAGACAAAATATGGATGTCGTC
AAACCTGAAATAATGCCTTTGATTTTTCAACAATTGGACATTGATAATTA
CGAAATAAAAGAAACAAATCCCAGATCAGTGTTCCGATTTTATGGAGCAA
ATCAAAATGGAAATAGCATTATTGCACATGTTCATGGATTTTATCCTTAT
TTATATGTCGAACCTCCTCCTGGCTTTTTAAAAGAGCATTGTGGCGAATT
TCGAAAAATTTTAAATCAGACACTCAAGCAAAGAGCGTCTCAACATCAAT
ATGCTGAAAATTTAATTTGTAAAGTAGAATATTGTTTGAAAGCAAGTAAG
ATATGAGTACAATAACTTTTGCGGTATTTCTCTCCATCGTATAATAATTA
AATAATAATGTAAAAAATATTTGATAAAAATAATATAAAATGAAATTCAC
TAATAGTAATTATTTTATCCCTGAAAAAATACTGCGAATTACCCAATTTT
TATCTATTTTAATTCTATTTATTTAGATATATATGGATTTAGTCAAAATC
TAAAAAAGGCATTTCTTAAAGTGACAGTTCCGGTACCAAGTTTAGTTGCC
ACTACTAAACGTGTACTTGTAGAGGGAATGACAATTCCTCATTATGGATA
TCACAACTTGGCATCTTTTGACACTGATATTGATTTTTCTGTTCGATTTA
TGGTTGATTCGGGTCTTGATGGATGTAGTTGGATAGAACTGCCCATTAAT
ACTTATACTGTACGACCGCAAACAGAGAAAATTTCATTTTGCCAATTAGA
AGTTGATGTTCAGTGGGACAAAATAATATGTCATTCTACTGAGGAGTGGC
CAAGTATTGCTCCGATTAGAATTTTAAGTTTTGATATAGAATGCGCTGGA
AGGAAAGGATCTTTTCCTGACGCGGATATTGATCCAGTCATCCAAATATC
CAATATTATTGTTAGATTTGGAGAATCAGAACCATTTATAAAAAATATAT
TTACATTGAATACATGCGCTCCAATTGTTGGCGCCCATGTGATTCCACAT
AAAACCGAAGCCGAATTGTTGGAGCATTGGTCTAACTTTATTCGGGAAAT
TGATCCTGATATAATTACCGGTTATAACACGCAAAACTTTGATTTGCCCT
ACCTCATCAATCGAGCCAACAAGTTAAACGTGAAAGGCTTTTCTTTGTTA
GGACGCATAAAATCTATGAGATCGAAAATCACTGAATCTCAGTCGCAATC
AAAACAAATGGGACGTAGAATAAATAAGAATGTTCACATGCCCGGTCGAA
TTCATTTCGATTTATATTTGATATTACTAAGAGATCATAAATTGCGCAGT
TATTCACTAAATGCTGTATCGTTCCATTTTCTGGAAGAACAAAAGGAGGA
TGTTGCCCATCAAATAATCACAGATTTACAAAATGGAGATGACCGAACTA
GGCATCGATTGGCAGTTTATTGTCTTAAAGATTCCGACTTAGTGTTGCGT
CTAATGAACAAATTGATGGTAGTTGTAAACTATGTTGAAATGGCTCGTGT
TACTGGAGTGCCTTTATCCTATTTGCTTTCTCGTGGACAGCAAATTAAGG
TCCTGTCTCAATTACTGAGAAAAGCGGCTATCGAAGACTTTGTGCTGCCG
GATTATCAATCAAAAGATGGCGATGAATTCACAGGTGCAACTGTAATCGA
GCCAATCAAAGGCTTTTATGACAAGCCAATCGCAACACTCGATTTTGCCA
GTTTGTACCCAAGCATTATGATGGCTCATAATCTTTGCTATACAACTCTG
TTGAAAAGCAAAGAATCAATACGGGAATTCGGTTTAACCGAAGATGATTA
TATAAAAACGCCTTGTGGTTCTTATTTTGTTAAAAGTTCTGTTAAAAAGG
GGCTTTTACCAAATATATTGATCCAGTTGATTGAGGCTAGGAAAAAAGCC
AAAAGAGAATTGGCAATTGAAACTGATCCATTTAAAAGGCAAGTCCTTGA
TGGTCGACAGTTGGCATTGAAAATCTCAGCAAATTCCGTTTATGGATTTA
CTGGAGCACAGCGTGGTCGTTTGCCATGTTTAAATATTTCATCTTCGGTA
ACTGCATTTGGTAGAGAAATGATCGATTTTACCAAGGAAATGGTTGAGAG
TTCATTCAATAAAGCTAACGGATATGTTCACGATGCAAAAGTTATTTATG
GAGATACCGATTCGGTTATGTGCTTATTCGGTGTGTCGACAGTTGTTGAA
GCTATGGCCCTAGGTAAGGAAGCGGCACAGAGTATATCTGGTAAGTTTAT
CGCACCAATCAAATTAGAATTTGAAAAAGTTTACTACCCGTATTTATTAA
TAAGCAAAAAGAGATATGCAGGAATGTATTATACAAAGCCAGATTCATTC
GATAAAATGGATTGTAAAGGCATGGAAACTGTCAGAAGAGACAATTGTCC
ATTGGTGGCAAATTTATTGAATACTTGCCTTCGAAAGTTGTTAATAAGCC
GTGATAAGAATGCTGCTTTGAGTCATGCTCAATCAGTGATAGCTGATTTG
TTATGCAATAGGATTGACATTTCCCAGTTGATTATCAGCAAAGAACTGAC
AAAAACTGACGAAGAATACACATCTAAGCAGCCACATTCCGAATTAGCAC
AGAAAATCAAAAAACGTGATCCTGGAAGTGCTCCTCAACTCGGCGATCGT
GTTCCGTATGTCATCACAACTCTCGGTGTCAAAGATAAAATATACACGAA
AGCCGAGGATCCAATTTACGTGTTAGACAATAACATTGGAATTGATACCG
ACTATTATCTAAAAAATCAATTGACTAACCCACTGTTGAGAATTTTTGAA
CCGATTTTCGGAAAAGAACTTGCAACAAGAAAGCTTCTACACGGTGAGCA
TGTTTTATCGAAATTTAAACAGCAATCAAATGTAGGTGCGCTTTCGAAAT
ACACTGTTATTAAACCGAAATGTATTGGATGCAAAAATGTTCTCAGAGAT
GACGAAAAATCTATTTGTGCCCACTGTAAGCCAAATGAATTGGCCATATA
TTGTCGAGAAAGCAAATATAATAAGTCACTGGAAATACGGTTTTCTCGTT
TGTGGACCGAATGTCAGCGTTGTTCTGGATCTCTTCACCAAGAAGTCTTG
TGTTCTAACCGAGATTGCCCGATATTCTATACGCGGAAGAAAGTGCAAAA
AGAACTGATTTCCCAAGATGAGATTATGGAGCGATTGAATCAAGATTTAT
CCTGGTGATAAGTCAAAATGTGAATCATTCGTTGTTGCACATATATTGTT
TGGTTGAATTCTCTAGTAGATTCATTATTGAAATAGTAAATATATATATA
TATATTTTC
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protein sequence of SMED30004407-orf-1

>SMED30004407-orf-1 ID=SMED30004407-orf-1|Name=SMED30004407-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=877bp
MTIPHYGYHNLASFDTDIDFSVRFMVDSGLDGCSWIELPINTYTVRPQTE
KISFCQLEVDVQWDKIICHSTEEWPSIAPIRILSFDIECAGRKGSFPDAD
IDPVIQISNIIVRFGESEPFIKNIFTLNTCAPIVGAHVIPHKTEAELLEH
WSNFIREIDPDIITGYNTQNFDLPYLINRANKLNVKGFSLLGRIKSMRSK
ITESQSQSKQMGRRINKNVHMPGRIHFDLYLILLRDHKLRSYSLNAVSFH
FLEEQKEDVAHQIITDLQNGDDRTRHRLAVYCLKDSDLVLRLMNKLMVVV
NYVEMARVTGVPLSYLLSRGQQIKVLSQLLRKAAIEDFVLPDYQSKDGDE
FTGATVIEPIKGFYDKPIATLDFASLYPSIMMAHNLCYTTLLKSKESIRE
FGLTEDDYIKTPCGSYFVKSSVKKGLLPNILIQLIEARKKAKRELAIETD
PFKRQVLDGRQLALKISANSVYGFTGAQRGRLPCLNISSSVTAFGREMID
FTKEMVESSFNKANGYVHDAKVIYGDTDSVMCLFGVSTVVEAMALGKEAA
QSISGKFIAPIKLEFEKVYYPYLLISKKRYAGMYYTKPDSFDKMDCKGME
TVRRDNCPLVANLLNTCLRKLLISRDKNAALSHAQSVIADLLCNRIDISQ
LIISKELTKTDEEYTSKQPHSELAQKIKKRDPGSAPQLGDRVPYVITTLG
VKDKIYTKAEDPIYVLDNNIGIDTDYYLKNQLTNPLLRIFEPIFGKELAT
RKLLHGEHVLSKFKQQSNVGALSKYTVIKPKCIGCKNVLRDDEKSICAHC
KPNELAIYCRESKYNKSLEIRFSRLWTECQRCSGSLHQEVLCSNRDCPIF
YTRKKVQKELISQDEIMERLNQDLSW*
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protein sequence of SMED30004407-orf-2

>SMED30004407-orf-2 ID=SMED30004407-orf-2|Name=SMED30004407-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=185bp
LFKSNLKKSKMSRRPNSFQKNKTYAPSSKISKFEVKNNDEDDVSMDEWNH
EPEEICFEMESNTETVKETDEFSKWARQNMDVVKPEIMPLIFQQLDIDNY
EIKETNPRSVFRFYGANQNGNSIIAHVHGFYPYLYVEPPPGFLKEHCGEF
RKILNQTLKQRASQHQYAENLICKVEYCLKASKI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: molecular function
TermDefinition
GO:0046872metal ion binding
GO:0051536iron-sulfur cluster binding
GO:00515394 iron, 4 sulfur cluster binding
GO:0003676nucleic acid binding
GO:0003887DNA-directed DNA polymerase activity
GO:0000166nucleotide binding
GO:0003677DNA binding
GO:00084083'-5' exonuclease activity
GO:0016740transferase activity
GO:0016779nucleotidyltransferase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR042087DNA_pol_B_C
IPR036397RNaseH_sf
IPR006172DNA-dir_DNA_pol_B
IPR006134DNA-dir_DNA_pol_B_multi_dom
IPR006133DNA-dir_DNA_pol_B_exonuc
IPR023211DNA_pol_palm_dom_sf
IPR012337RNaseH-like_sf
IPR025687Znf-C4pol
IPR017964DNA-dir_DNA_pol_B_CS
Vocabulary: biological process
TermDefinition
GO:0006260DNA replication
GO:0071897DNA biosynthetic process
GO:0090305nucleic acid phosphodiester bond hydrolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 657..677
NoneNo IPR availableGENE3DG3DSA:1.10.287.690coord: 229..281
e-value: 8.6E-79
score: 266.1
NoneNo IPR availableTIGRFAMTIGR00592TIGR00592coord: 11..545
e-value: 6.9E-88
score: 293.9
NoneNo IPR availableGENE3DG3DSA:1.10.287.690coord: 102..150
e-value: 2.2E-9
score: 39.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 468..489
NoneNo IPR availablePANTHERPTHR10322DNA POLYMERASE CATALYTIC SUBUNITcoord: 2..673
NoneNo IPR availablePANTHERPTHR10322:SF23DNA POLYMERASE DELTA CATALYTIC SUBUNITcoord: 2..673
NoneNo IPR availableCDDcd05533POLBc_deltacoord: 153..547
e-value: 0.0
score: 735.997
NoneNo IPR availableSUPERFAMILYSSF56672DNA/RNA polymerasescoord: 141..550
IPR006172DNA-directed DNA polymerase, family BPRINTSPR00106DNAPOLBcoord: 172..185
score: 58.69
coord: 265..277
score: 58.32
coord: 326..334
score: 76.12
IPR006172DNA-directed DNA polymerase, family BSMARTSM00486polmehr3coord: 1..340
e-value: 4.1E-77
score: 272.2
IPR042087DNA polymerase family B, C-terminal domainGENE3DG3DSA:1.10.132.60coord: 402..558
e-value: 4.7E-45
score: 155.1
IPR036397Ribonuclease H superfamilyGENE3DG3DSA:3.30.420.10coord: 1..101
e-value: 5.6E-34
score: 119.3
IPR025687C4-type zinc-finger of DNA polymerase deltaPFAMPF14260zf-C4polcoord: 586..657
e-value: 1.4E-15
score: 57.5
IPR023211DNA polymerase, palm domain superfamilyGENE3DG3DSA:3.90.1600.10coord: 155..385
e-value: 8.6E-79
score: 266.1
IPR006133DNA-directed DNA polymerase, family B, exonuclease domainPFAMPF03104DNA_pol_B_exo1coord: 5..51
e-value: 3.3E-5
score: 23.2
IPR006134DNA-directed DNA polymerase, family B, multifunctional domainPFAMPF00136DNA_pol_Bcoord: 115..547
e-value: 2.7E-145
score: 484.6
IPR017964DNA-directed DNA polymerase, family B, conserved sitePROSITEPS00116DNA_POLYMERASE_Bcoord: 328..336
IPR012337Ribonuclease H-like superfamilySUPERFAMILYSSF53098Ribonuclease H-likecoord: 16..137