GCR079

Overview
NameGCR079
Smed IDSMED30004403
Length (bp)1579
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR079 (SMED30004403) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR079 (SMED30004403) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR079 (SMED30004403) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR079 (SMED30004403) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004403

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30004403h1SMcG0019750 dd_Smed_v4_11910_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
prepharyngeal regionSMED30004403h1SMcG0019750 dd_Smed_v6_11910_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GCR079 vs. Ensembl Celegans
Match: frpr-6 (FMRFamide Peptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q8I7K6])

HSP 1 Score: 53.1434 bits (126), Expect = 2.623e-7
Identity = 58/228 (25.44%), Postives = 110/228 (48.25%), Query Frame = 3 
Query:  252 RYGSLLWL---MSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQG-----LFI--SSQYF----------SVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISRE--YNDVSKKCDIRGSVVNTRNWFIFATG---TEILISALLPFATL-IVNILVIIELKSITSKTTLIKPSV 857
             Y S+++L   + GLV N L+  ++T P +R+S T A  L  +++ D IF LL+ L     S+      +P+W  ++       +FI  S+++F          S+ ++++ ++ER+IAVC P +    CT + ++   I     ++IL   +    +  Y+D       RG +    +W+ + +      ++   LLPF  + I N+ VI EL+    +  L+  S+
Sbjct:    8 EYVSMIYLPIILVGLVGNGLSLYVYTTPNMRKS-TVAFLLYSLSICD-IFVLLFALPLYSISY------LPIWDNVYGAWSMRRMFIAFSTKFFYPLCMTAKTASLYIMVVITVERWIAVCRPLQVHIWCTFKNSVRIVIAIITFSIILNFPKFFEYQIGYSDALGYWPKRGILDAEEHWWYYISYFIIISVIFDYLLPFVIMFIANMKVINELRKSRKERALLTTSL 227
[Close]
BLAST of GCR079 vs. Ensembl Celegans
Match: frpr-3 (FMRFamide Peptide Receptor family; VRFamide receptor 1 [Source:UniProtKB/TrEMBL;Acc:G5EG36])

HSP 1 Score: 48.521 bits (114), Expect = 8.316e-6
Identity = 36/118 (30.51%), Postives = 59/118 (50.00%), Query Frame = 3 
Query:  264 LLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLF-----ISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALV 602
            +L    G++ N ++F I+TR   R+ + +   LA ++MSD    +L I  +  T      Q IP        ++     I  Q  SV L++  +++RY+AVCHPF     CT  +AL+
Sbjct:   14 VLMCFGGILTNFISFYIYTRKTFRKKSINV-LLAALSMSDLCVCVLAIPVFASTQLQ---QVIPPTITAMIMVYLYPVTIMFQSVSVWLLVSITIDRYLAVCHPFMVNTYCTRNRALI 127
[Close]
BLAST of GCR079 vs. Ensembl Fly
Match: CNMaR (gene:FBgn0053696 transcript:FBtr0091684)

HSP 1 Score: 59.3066 bits (142), Expect = 4.802e-9
Identity = 40/125 (32.00%), Postives = 71/125 (56.80%), Query Frame = 3 
Query:  243 YLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIF-------WLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKA 596
            ++H+Y   +   +G + N L+  ++ R +LR+ ++S  YLA +A+SDT F       WL ++      + DI  Q+   +C+ F      + + SV  V+ F++ER+IAV +P KR+ +CT R+A
Sbjct:   60 FVHQYYIPVLCCTGSIGNILSVFVFFRTKLRKLSSSF-YLAALAVSDTCFLAGLFAQWLNFL------NVDIYNQNY--FCQFFTFFSYLASFCSVWFVVAFTVERFIAVIYPLKRQTMCTVRRA 175
[Close]
BLAST of GCR079 vs. Ensembl Fly
Match: FMRFaR (gene:FBgn0035385 transcript:FBtr0340706)

HSP 1 Score: 56.225 bits (134), Expect = 4.458e-8
Identity = 41/128 (32.03%), Postives = 63/128 (49.22%), Query Frame = 3 
Query:  282 GLVLNTLAFIIWTRPRLRRSNTSANYL-AGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGL------FISSQYFSVPLV---------LIFSLERYIAVCHPFKREKLCTHRKALVNTIVC 617
            G++ N ++ II +RP++R   +S NYL  G+A  DT+  +  IL +        +  I  +   F G       FIS   F + ++            +LERY+AVCHP K   LCT+ +A +  IVC
Sbjct:  127 GILGNIISMIILSRPQMR---SSINYLLTGLARCDTVLIITSILLF-------GIPSIYPYTGHFFGYYNYVYPFISPAVFPIGMIAQTASIYMTFTVTLERYVAVCHPLKARALCTYGRAKIYFIVC 244
[Close]
BLAST of GCR079 vs. Ensembl Fly
Match: FMRFaR (gene:FBgn0035385 transcript:FBtr0332085)

HSP 1 Score: 56.225 bits (134), Expect = 4.458e-8
Identity = 41/128 (32.03%), Postives = 63/128 (49.22%), Query Frame = 3 
Query:  282 GLVLNTLAFIIWTRPRLRRSNTSANYL-AGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGL------FISSQYFSVPLV---------LIFSLERYIAVCHPFKREKLCTHRKALVNTIVC 617
            G++ N ++ II +RP++R   +S NYL  G+A  DT+  +  IL +        +  I  +   F G       FIS   F + ++            +LERY+AVCHP K   LCT+ +A +  IVC
Sbjct:  127 GILGNIISMIILSRPQMR---SSINYLLTGLARCDTVLIITSILLF-------GIPSIYPYTGHFFGYYNYVYPFISPAVFPIGMIAQTASIYMTFTVTLERYVAVCHPLKARALCTYGRAKIYFIVC 244
[Close]
BLAST of GCR079 vs. Ensembl Fly
Match: FMRFaR (gene:FBgn0035385 transcript:FBtr0072980)

HSP 1 Score: 56.225 bits (134), Expect = 4.458e-8
Identity = 41/128 (32.03%), Postives = 63/128 (49.22%), Query Frame = 3 
Query:  282 GLVLNTLAFIIWTRPRLRRSNTSANYL-AGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGL------FISSQYFSVPLV---------LIFSLERYIAVCHPFKREKLCTHRKALVNTIVC 617
            G++ N ++ II +RP++R   +S NYL  G+A  DT+  +  IL +        +  I  +   F G       FIS   F + ++            +LERY+AVCHP K   LCT+ +A +  IVC
Sbjct:  127 GILGNIISMIILSRPQMR---SSINYLLTGLARCDTVLIITSILLF-------GIPSIYPYTGHFFGYYNYVYPFISPAVFPIGMIAQTASIYMTFTVTLERYVAVCHPLKARALCTYGRAKIYFIVC 244
[Close]
BLAST of GCR079 vs. Ensembl Zebrafish
Match: lpar6a (lysophosphatidic acid receptor 6a [Source:ZFIN;Acc:ZDB-GENE-061013-343])

HSP 1 Score: 49.2914 bits (116), Expect = 7.365e-6
Identity = 33/118 (27.97%), Postives = 60/118 (50.85%), Query Frame = 3 
Query:  276 MSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWL---LYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCG 620
            M GL+ N  A  I+    L+  N +  Y+  + +SD +F L   L + +++Q +W        + C++   LF ++ Y S+  +   S++R++A+ +PF+   L T R A    IVC 
Sbjct:   39 MVGLITNVAAMYIFM-CSLKLRNETTTYMMNLVVSDLLFVLTLPLRVFYFVQQNWPFG----SLLCKLSVSLFYTNMYGSILFLTCISVDRFLAIVYPFRSRGLRTKRNA---KIVCA 148
[Close]
BLAST of GCR079 vs. Ensembl Zebrafish
Match: lpar6a (lysophosphatidic acid receptor 6a [Source:ZFIN;Acc:ZDB-GENE-061013-343])

HSP 1 Score: 49.2914 bits (116), Expect = 7.365e-6
Identity = 33/118 (27.97%), Postives = 60/118 (50.85%), Query Frame = 3 
Query:  276 MSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWL---LYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCG 620
            M GL+ N  A  I+    L+  N +  Y+  + +SD +F L   L + +++Q +W        + C++   LF ++ Y S+  +   S++R++A+ +PF+   L T R A    IVC 
Sbjct:   39 MVGLITNVAAMYIFM-CSLKLRNETTTYMMNLVVSDLLFVLTLPLRVFYFVQQNWPFG----SLLCKLSVSLFYTNMYGSILFLTCISVDRFLAIVYPFRSRGLRTKRNA---KIVCA 148
[Close]
BLAST of GCR079 vs. Ensembl Xenopus
Match: p2ry2 (purinergic receptor P2Y, G-protein coupled, 2 [Source:NCBI gene;Acc:394636])

HSP 1 Score: 51.2174 bits (121), Expect = 2.258e-6
Identity = 54/207 (26.09%), Postives = 99/207 (47.83%), Query Frame = 3 
Query:  282 GLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWL---LYILHYMQ-TSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSI----SREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSS 878
            GL+LN LA  I+   R++  N S  Y+  +A+SD ++ +   L + +Y Q  +W   V      C++ + LF ++ Y S+  +L  S+ R++ +C+P K       R A + ++V  V  ++  C   I    +   N  S  C    SV    N+ +++T  ++ +   +PF T+I+   ++          TL+KPS     TS+
Sbjct:   36 GLILNILALYIFLF-RIKPWNASTTYMFNLAISDMMYVISLPLLVYYYSQGDNWPFGVA----LCKIVKFLFYTNMYCSISFLLCISIHRFLGICYPMKSLGWLKVRNARIISVV--VWVIVSACQSPILYFVTTSSNGDSTTCHDTSSVDLFDNFVVYST-VDLALLFCVPFITIIICYCLMAR--------TLMKPSAATAQTSA 226
[Close]
BLAST of GCR079 vs. Ensembl Mouse
Match: Gpr35 (G protein-coupled receptor 35 [Source:MGI Symbol;Acc:MGI:1929509])

HSP 1 Score: 50.8322 bits (120), Expect = 4.020e-7
Identity = 28/94 (29.79%), Postives = 56/94 (59.57%), Query Frame = 3 
Query:  315 WTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKA 596
            WT  R+  +N +   +A + +  ++ ++LY L Y  +       D PV C++ QG++++++Y S+ LV   +++RY+AV HP +  +L + R+A
Sbjct:   49 WTETRIYMTNLA---VADLCLLCSLPFVLYSLKYSSS-------DTPV-CQLSQGIYLANRYMSISLVTAIAVDRYVAVRHPLRARELRSPRQA 131
[Close]
BLAST of GCR079 vs. Ensembl Mouse
Match: Gpr35 (G protein-coupled receptor 35 [Source:MGI Symbol;Acc:MGI:1929509])

HSP 1 Score: 50.8322 bits (120), Expect = 1.866e-6
Identity = 28/94 (29.79%), Postives = 56/94 (59.57%), Query Frame = 3 
Query:  315 WTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKA 596
            WT  R+  +N +   +A + +  ++ ++LY L Y  +       D PV C++ QG++++++Y S+ LV   +++RY+AV HP +  +L + R+A
Sbjct:   49 WTETRIYMTNLA---VADLCLLCSLPFVLYSLKYSSS-------DTPV-CQLSQGIYLANRYMSISLVTAIAVDRYVAVRHPLRARELRSPRQA 131
[Close]
BLAST of GCR079 vs. Ensembl Mouse
Match: Gpr35 (G protein-coupled receptor 35 [Source:MGI Symbol;Acc:MGI:1929509])

HSP 1 Score: 50.8322 bits (120), Expect = 1.866e-6
Identity = 28/94 (29.79%), Postives = 56/94 (59.57%), Query Frame = 3 
Query:  315 WTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKA 596
            WT  R+  +N +   +A + +  ++ ++LY L Y  +       D PV C++ QG++++++Y S+ LV   +++RY+AV HP +  +L + R+A
Sbjct:   49 WTETRIYMTNLA---VADLCLLCSLPFVLYSLKYSSS-------DTPV-CQLSQGIYLANRYMSISLVTAIAVDRYVAVRHPLRARELRSPRQA 131
[Close]
BLAST of GCR079 vs. Ensembl Mouse
Match: Gpr35 (G protein-coupled receptor 35 [Source:MGI Symbol;Acc:MGI:1929509])

HSP 1 Score: 50.8322 bits (120), Expect = 1.866e-6
Identity = 28/94 (29.79%), Postives = 56/94 (59.57%), Query Frame = 3 
Query:  315 WTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKA 596
            WT  R+  +N +   +A + +  ++ ++LY L Y  +       D PV C++ QG++++++Y S+ LV   +++RY+AV HP +  +L + R+A
Sbjct:   49 WTETRIYMTNLA---VADLCLLCSLPFVLYSLKYSSS-------DTPV-CQLSQGIYLANRYMSISLVTAIAVDRYVAVRHPLRARELRSPRQA 131
[Close]
BLAST of GCR079 vs. UniProt/SwissProt
Match: sp|Q9VZW5|FMAR_DROME (FMRFamide receptor OS=Drosophila melanogaster OX=7227 GN=FMRFaR PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 4.469e-7
Identity = 41/128 (32.03%), Postives = 63/128 (49.22%), Query Frame = 3 
Query:  282 GLVLNTLAFIIWTRPRLRRSNTSANYL-AGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGL------FISSQYFSVPLV---------LIFSLERYIAVCHPFKREKLCTHRKALVNTIVC 617
            G++ N ++ II +RP++R   +S NYL  G+A  DT+  +  IL +        +  I  +   F G       FIS   F + ++            +LERY+AVCHP K   LCT+ +A +  IVC
Sbjct:  127 GILGNIISMIILSRPQMR---SSINYLLTGLARCDTVLIITSILLF-------GIPSIYPYTGHFFGYYNYVYPFISPAVFPIGMIAQTASIYMTFTVTLERYVAVCHPLKARALCTYGRAKIYFIVC 244
[Close]
BLAST of GCR079 vs. TrEMBL
Match: A0A193KUK6 (GCR079 OS=Schmidtea mediterranea OX=79327 GN=gcr079 PE=2 SV=1)

HSP 1 Score: 811.987 bits (2096), Expect = 0.000e+0
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 3 
Query:  156 MESEINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQNAKRFKGPDDTSIGNSYFSNRNGHNQRVNHTEMLRKQAEH 1388
            MESEINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKSSTILLLSVSFLLIVTTLPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQNAKRFKGPDDTSIGNSYFSNRNGHNQRVNHTEMLRKQAEH
Sbjct:    1 MESEINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKSSTILLLSVSFLLIVTTLPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQNAKRFKGPDDTSIGNSYFSNRNGHNQRVNHTEMLRKQAEH 411
[Close]
BLAST of GCR079 vs. TrEMBL
Match: A0A504YH16 (Rhodopsin orphan GPCR (Fragment) OS=Fasciola gigantica OX=46835 GN=FGIG_08547 PE=3 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.211e-49
Identity = 120/353 (33.99%), Postives = 198/353 (56.09%), Query Frame = 3 
Query:  216 RKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDI---PVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSI---SREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQG----STEKLNIKKHSISQQNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFR 1244
            R +L S    L R   L+WL++GL  NTL+ +IWT    RR NTSA YL+ +A++D       + ++++  W  CV+ I   PV C+++Q L I SQY+S  LV  F+LERY+AVC PFKR KLCTH++AL       V  L++ C   +   +  +   + +C +R S++         T  E++ S ++P A L++NILV+ E++ +         S+T    + TS S+  L+ +++      S   +    +++  +++E S+F ++TI+L+ +SF LI  T+P G VY  QF     ++ L+ E  + DP W + I+   +KEV+D     H+++NF IY +T + FR
Sbjct:   22 RPFLPSDILTLVRCLMLIWLITGLFGNTLSLVIWTTKHQRRRNTSAIYLSALAVTDLTLIFCLLNYHLERYW--CVRGITSFPVLCQIYQCLSIFSQYYSTALVFGFTLERYLAVCFPFKRHKLCTHKRALF-----AVLCLLVICCMPMLFQAALWTFENGECTMRTSMIQDHRVTQTLTAQEVIFSLIVPLAALVLNILVLFEMRRLVHSRAAPSHSITG--RNGTSLSKSSLKQRKSSAFSVTSVASVQTNANAVEAKSRESSSFTATTIMLVILSFYLIACTVPPGIVYLTQFYLTAPDDCLTAEAARQDPIWQQIISSTTVKEVIDVLCSSHYALNFFIYILTYKGFR 365
[Close]
BLAST of GCR079 vs. TrEMBL
Match: A0A4E0RIU3 (Rhodopsin orphan GPCR OS=Fasciola hepatica OX=6192 GN=D915_001166 PE=3 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 2.765e-48
Identity = 115/337 (34.12%), Postives = 192/337 (56.97%), Query Frame = 3 
Query:  264 LLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDI---PVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSI---SREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQG----STEKLNIKKHSISQQNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFR 1244
            L+WL++GL  NTL+ +IWT    RR NTSA YL+ +A++D       + ++++  W  CV+ I   PV C+++Q L I SQY+S  LV  F+LERY+AVC PFKR KLCTH++AL       V  L++ C   +   +  +   + +C +R S++         T  E++ S ++P A L++NILV+ E++ +         S+T    + TS S+  L+ +++      S   +    +++  +++E S+F ++TI+L+ +SF LI  T+P G VY  QF     ++ L+ E  + DP W + I+   +KEV+D     H+++NF IY +T + FR
Sbjct:   45 LIWLITGLFGNTLSLVIWTTKHQRRRNTSAIYLSALAITDLTLIFCLLNYHLERYW--CVRGITSFPVLCQIYQCLSIFSQYYSTALVFGFTLERYLAVCFPFKRHKLCTHKRALF-----AVLCLLVICCMPMLFQAALWTFENGECTMRTSMIQDHRVTQTLTAQEVIFSLIVPLAALVLNILVLFEMRRLVHSRAAPSHSITG--RNGTSLSKSSLKQRKSSAFSVTSVASVQTNANAVEAKSRESSSFTATTIMLVILSFYLIACTVPPGIVYLTQFYLTAPDDCLTAEAARQDPIWQQIISSTTVKEVIDVLCSSHYALNFFIYILTYKGFR 372
[Close]
BLAST of GCR079 vs. TrEMBL
Match: A0A2H1CT95 (7 transmembrane receptor OS=Fasciola hepatica OX=6192 GN=D915_01727 PE=3 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 3.499e-48
Identity = 115/337 (34.12%), Postives = 192/337 (56.97%), Query Frame = 3 
Query:  264 LLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDI---PVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSI---SREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQG----STEKLNIKKHSISQQNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFR 1244
            L+WL++GL  NTL+ +IWT    RR NTSA YL+ +A++D       + ++++  W  CV+ I   PV C+++Q L I SQY+S  LV  F+LERY+AVC PFKR KLCTH++AL       V  L++ C   +   +  +   + +C +R S++         T  E++ S ++P A L++NILV+ E++ +         S+T    + TS S+  L+ +++      S   +    +++  +++E S+F ++TI+L+ +SF LI  T+P G VY  QF     ++ L+ E  + DP W + I+   +KEV+D     H+++NF IY +T + FR
Sbjct:   55 LIWLITGLFGNTLSLVIWTTKHQRRRNTSAIYLSALAITDLTLIFCLLNYHLERYW--CVRGITSFPVLCQIYQCLSIFSQYYSTALVFGFTLERYLAVCFPFKRHKLCTHKRALF-----AVLCLLVICCMPMLFQAALWTFENGECTMRTSMIQDHRVTQTLTAQEVIFSLIVPLAALVLNILVLFEMRRLVHSRAAPSHSITG--RNGTSLSKSSLKQRKSSAFSVTSVASVQTNANAVEAKSRESSSFTATTIMLVILSFYLIACTVPPGIVYLTQFYLTAPDDCLTAEAARQDPIWQQIISSTTVKEVIDVLCSSHYALNFFIYILTYKGFR 382
[Close]
BLAST of GCR079 vs. TrEMBL
Match: A0A3P7L2X2 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS7516 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 1.186e-47
Identity = 115/351 (32.76%), Postives = 197/351 (56.13%), Query Frame = 3 
Query:  210 LIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLL----YILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTE 1250
            L+++++    FYL  Y S +W   GL+ N ++F+IW   R R  N+SA YLA +A+SD    LL    Y+  ++     I V  +   C++FQG+F+  QY+S+ L   F+LER+IA+C+PFKR KLC+ R+AL+   V  +  +++  +   +  +  V  +C +     +  +++I+ +  E + S L+P ATL+ NILV+ E+  +     ++K   ++     +ST   H+   +       L  K    S   KE+S F ++T++L+ +SF LI+ TLP G V+ +Q      +E L+ E++   P W ++  +   KE+VDF    H++ NF+IY ITG+SFR++
Sbjct:   22 LLKRFV-PDAFYLKLYASPVWFFVGLIGNAISFLIWVSHRQRGKNSSAIYLAALALSDFFLILLLFSDYLRRFLFFESPITVNGL---CQLFQGVFLFLQYYSIALTFGFTLERWIAICYPFKRHKLCSSRRALIG--VSALAVIVISFNVFYAAAWEIVDHECVLAARWQDDSSFYIYLSTVECVFSLLVPLATLVFNILVLREIGKLVRSKIIVKGQNSSLFRQRSSTKSAHVPEHQ-----LILGAKHSGHSASAKEQSNFHAATVMLVILSFYLILCTLPVGIVFVVQMRFTSFSEFLTDEEVLQHPQWREYFGFLRAKEIVDFLCSSHYACNFIIYLITGKSFRSQ 361
[Close]
BLAST of GCR079 vs. Ensembl Cavefish
Match: ENSAMXT00000031230.1 (pep primary_assembly:Astyanax_mexicanus-2.0:8:15661407:15665947:-1 gene:ENSAMXG00000043727.1 transcript:ENSAMXT00000031230.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 1.474e-8
Identity = 41/127 (32.28%), Postives = 71/127 (55.91%), Query Frame = 3 
Query:  273 LMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYI-----LHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALIL 638
            L+ G+ LN  +  +++R RL RSN +  +L  +AM+DT FWLL +      H +   W +    +P +C++ + L+ +  Y S+  V   S++RY+A+ HP +   L T R+A++  I   + AL L
Sbjct:   39 LILGVPLNVFSLWVFSR-RLSRSNRTILFLHNLAMADT-FWLLALPFLIQYHLVGMQWSL---GLP-FCKVIRLLYHNYFYLSIFFVTCLSVDRYLAIVHPLRSPVLLTRRRAVILCISVWLLALAL 159
[Close]
BLAST of GCR079 vs. Ensembl Cavefish
Match: lpar6b (lysophosphatidic acid receptor 6b [Source:ZFIN;Acc:ZDB-GENE-110408-67])

HSP 1 Score: 51.9878 bits (123), Expect = 9.108e-7
Identity = 44/152 (28.95%), Postives = 79/152 (51.97%), Query Frame = 3 
Query:  225 LWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWL---LYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYN 671
            L+SS F      SL++++ GL+LN +A  I+TR  L++ N +  Y+  + +SDT+F L     I +++  +W          C++   LF ++ Y S+  +     +R++A+ +PF    L T RKA      CG+  ++L  S S+  + N
Sbjct:   44 LYSSVF------SLVFIL-GLLLNMVALYIFTR-TLQQWNETTIYMMNLVVSDTLFVLSLPFRIYYFVNQTWPFG----HTLCKISVSLFYTNLYGSILFLTCICADRFLAIVYPFASRSLRTKRKA--KMACCGIWIIVLSGSFSVGFKIN 181
[Close]
BLAST of GCR079 vs. Ensembl Medaka
Match: lpar6b (lysophosphatidic acid receptor 6-like [Source:NCBI gene;Acc:105355627])

HSP 1 Score: 54.6842 bits (130), Expect = 1.147e-7
Identity = 35/129 (27.13%), Postives = 69/129 (53.49%), Query Frame = 3 
Query:  255 YGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWL---LYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTAL 632
            Y S+++L+  L   T  +I   R +LR  N +  Y+  +A+SD++F       ++++ + +W       PV+C++   LF ++ Y S+  +   S++R++A+ HPF+   + T R A +  I+  V  L
Sbjct:   24 YFSVVFLLGFLFNMTAMYIFGCRLKLR--NETTTYMINLAVSDSLFVFTLPFRVVYFTKRNWIFG----PVFCKISTALFYTNMYGSILFLTCISVDRFLAIVHPFRSRAIRTKRNAKLTCILVWVMIL 146
[Close]
BLAST of GCR079 vs. Planmine SMEST
Match: SMESG000014330.1 (SMESG000014330.1)

HSP 1 Score: 811.987 bits (2096), Expect = 0.000e+0
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 3 
Query:  156 MESEINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQNAKRFKGPDDTSIGNSYFSNRNGHNQRVNHTEMLRKQAEH 1388
            MESEINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKSSTILLLSVSFLLIVTTLPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQNAKRFKGPDDTSIGNSYFSNRNGHNQRVNHTEMLRKQAEH
Sbjct:    1 MESEINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKSSTILLLSVSFLLIVTTLPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQNAKRFKGPDDTSIGNSYFSNRNGHNQRVNHTEMLRKQAEH 411
[Close]
BLAST of GCR079 vs. Planmine SMEST
Match: SMESG000007702.1 (SMESG000007702.1)

HSP 1 Score: 196.438 bits (498), Expect = 8.256e-57
Identity = 139/379 (36.68%), Postives = 209/379 (55.15%), Query Frame = 3 
Query:  249 HRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLT----SSTSTSEQHLQIQRNQGSTEKLNIKKHS---ISQ----------------------QNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQNAKRFKGPDD 1298
            HR+ + + L +GL+ N+++FIIWT PR+RR+N+SA YL+ +++SD I  L + +HY++ +W I V D    CEMFQ +++  QY S PLVL F+LER+IAVC+PFKRE+ CT ++A +   +  +  L++G  Q+ +   N+  + C  R S   +     F T  E L+S  +P  TLI NILVI EL+SI        P+          S  S SE+     +N+   E  ++   +   +SQ                      Q+     F+S+TI+LL+VSF  I+TTLP+G VY  QF    GNE LS ++++ DP+W  F  +Q +KE VD FS+ H+  N +IY +TG  FR     + +  K   G D 
Sbjct:   27 HRWITPVLLFTGLIGNSVSFIIWTNPRIRRNNSSATYLSALSLSDLILLLTHFMHYLKFAWRIKVFDYAGVCEMFQMIYLMFQYNSTPLVLAFTLERWIAVCYPFKRERWCTSKRAYLAITLIFIIMLLIGVVQAFAWSTNNEQRVCKFRDSWQRSMLNQGFTTTVEFLMSGGVPICTLIFNILVIYELRSIVLNPLKQDPNKVNKKNRENKSQLSVSEKISFSSKNEKGLESFDVNAENSNLLSQPVGKSVIKRYTFVNNAHKMQRFQSSGGVNFRSTTIMLLTVSFYQIITTLPSGAVYVSQFFISGGNEFLSDDEIENDPNWRTFFRHQTMKEFVDLFSLTHYCFNVLIYLLTGRQFRKYLLQILKLDKCIYGADS 405
[Close]
BLAST of GCR079 vs. Planmine SMEST
Match: SMESG000031888.1 (SMESG000031888.1)

HSP 1 Score: 163.31 bits (412), Expect = 5.931e-45
Identity = 121/384 (31.51%), Postives = 198/384 (51.56%), Query Frame = 3 
Query:  243 YLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNW---FIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKSAFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFRTEFYLLFQN-AKRFKGPDDTSIGNSYFSNRNGHNQRVNHTEMLRKQA 1382
            Y+ RY +L+WL+ G + N  +FIIW  P++RR N+SA YLA ++++D +  +  ++  +Q SW+  V     +C ++  L   SQ FS  LVL F++ER++A+C+PFKR+ LCT ++A++ +    +  L           YN+    C       N  ++   +IFA  TEI+ S  +P   L  NILVI E+  IT              T S     Q                  H  +Q+   + +F  +TI+LL+VSF L  TTLP G ++ALQ V  + +E +++ ++  DP W  +    I+K V+D   + H+++NFVIY +TG++FR +F    +    +FK  D+      +FSN      + N  E+ R+ +
Sbjct:   24 YIDRYMTLIWLLIGYIGNIFSFIIWIEPKMRRENSSAIYLAALSLNDFLVLVFVMIQDLQYSWNKNVLRYS-FCSIYMVLSTCSQLFSPVLVLAFTIERWVAICYPFKRQSLCTPKRAIIVSFTLYLFVLCYNIYNGFMWIYNERFDSCVFNQLETNGTDFQKQYIFA--TEIVFSGCVPLLILGFNILVIKEMYRITKCNR----------TKSMGNCPQF-----------------HDPNQRFNNERSFLFTTIMLLAVSFYLFFTTLPGGIMFALQQVITMPDENMNLSKIPSDPKWQFYFRVSIIKAVIDELCISHYALNFVIYLVTGKAFRKQFIDKIRKIITKFKLTDN------FFSNELVSLNKTNRMELTRENS 371
[Close]
BLAST of GCR079 vs. Planmine SMEST
Match: SMESG000018637.1 (SMESG000018637.1)

HSP 1 Score: 161.384 bits (407), Expect = 3.110e-44
Identity = 126/363 (34.71%), Postives = 193/363 (53.17%), Query Frame = 3 
Query:  168 INRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGV---TALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKS-AFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFR 1244
            +N T    N  TL  IR+     ++ L  Y   ++L  G++ N ++F++W   +LRR+N+SA Y+A +A++D    +  I++Y++ SW   + DI   C+ +  +F   QY S+ LV  F++ER+IAVC+PF+RE+ CT  +A    I CG+   +  I          Y  +   C +    +    +  ++   EIL SALLP  TL VNI V++E+  I S+T +  P ++A          Q+ +I   +        +KHS   +N E S +FKS+TI LL VSF  I+TTLP G VY  Q   P GN T SV+QM+ DP W K       K+++D  S+ H+  N VIY  +   FR
Sbjct:    1 MNETSATSNEKTLLEIRQIFLPISYQLQLYSIPIFLTVGILGNPISFVVWVSRKLRRNNSSAVYIAMLAINDFFVVICSIIYYLRHSWGFGIIDIRGICKFYMVIFTFLQYNSILLVFGFTIERWIAVCYPFRRERCCTENRAW---IACGIIFSSVFISVFPLFFVWHYQPL---CSVPKHWIENTYFVTYSLTLEILFSALLPVITLAVNIRVVVEMYRI-SETIVTFPKLSA--------VNQNFEIPSQKS-------RKHS---KNTENSISFKSTTITLLLVSFFQILTTLPLGAVYICQIFYPEGNAT-SVKQMQNDPIWKKHFQMHGAKDIIDLLSLSHYCFNIVIYLASSHLFR 337
[Close]
BLAST of GCR079 vs. Planmine SMEST
Match: SMESG000018637.1 (SMESG000018637.1)

HSP 1 Score: 162.155 bits (409), Expect = 3.411e-44
Identity = 126/364 (34.62%), Postives = 194/364 (53.30%), Query Frame = 3 
Query:  165 EINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLAFIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIPVWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVNTIVCGV---TALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEILISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQIQRNQGSTEKLNIKKHSISQQNKEKS-AFKXXXXXXXXXXXXXXXXXXPTGFVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSINFVIYYITGESFR 1244
            ++N T    N  TL  IR+     ++ L  Y   ++L  G++ N ++F++W   +LRR+N+SA Y+A +A++D    +  I++Y++ SW   + DI   C+ +  +F   QY S+ LV  F++ER+IAVC+PF+RE+ CT  +A    I CG+   +  I          Y  +   C +    +    +  ++   EIL SALLP  TL VNI V++E+  I S+T +  P ++A          Q+ +I   +        +KHS   +N E S +FKS+TI LL VSF  I+TTLP G VY  Q   P GN T SV+QM+ DP W K       K+++D  S+ H+  N VIY  +   FR
Sbjct:   45 QMNETSATSNEKTLLEIRQIFLPISYQLQLYSIPIFLTVGILGNPISFVVWVSRKLRRNNSSAVYIAMLAINDFFVVICSIIYYLRHSWGFGIIDIRGICKFYMVIFTFLQYNSILLVFGFTIERWIAVCYPFRRERCCTENRAW---IACGIIFSSVFISVFPLFFVWHYQPL---CSVPKHWIENTYFVTYSLTLEILFSALLPVITLAVNIRVVVEMYRI-SETIVTFPKLSA--------VNQNFEIPSQKS-------RKHS---KNTENSISFKSTTITLLLVSFFQILTTLPLGAVYICQIFYPEGNAT-SVKQMQNDPIWKKHFQMHGAKDIIDLLSLSHYCFNIVIYLASSHLFR 382
The following BLAST results are available for this feature:
BLAST of GCR079 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR079 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
frpr-62.623e-725.44FMRFamide Peptide Receptor family [Source:UniProt... [more]
frpr-38.316e-630.51FMRFamide Peptide Receptor family; VRFamide recept... [more]
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BLAST of GCR079 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
CNMaR4.802e-932.00gene:FBgn0053696 transcript:FBtr0091684[more]
FMRFaR4.458e-832.03gene:FBgn0035385 transcript:FBtr0340706[more]
FMRFaR4.458e-832.03gene:FBgn0035385 transcript:FBtr0332085[more]
FMRFaR4.458e-832.03gene:FBgn0035385 transcript:FBtr0072980[more]
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BLAST of GCR079 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
lpar6a7.365e-627.97lysophosphatidic acid receptor 6a [Source:ZFIN;Acc... [more]
lpar6a7.365e-627.97lysophosphatidic acid receptor 6a [Source:ZFIN;Acc... [more]
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BLAST of GCR079 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
p2ry22.258e-626.09purinergic receptor P2Y, G-protein coupled, 2 [Sou... [more]
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BLAST of GCR079 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Gpr354.020e-729.79G protein-coupled receptor 35 [Source:MGI Symbol;A... [more]
Gpr351.866e-629.79G protein-coupled receptor 35 [Source:MGI Symbol;A... [more]
Gpr351.866e-629.79G protein-coupled receptor 35 [Source:MGI Symbol;A... [more]
Gpr351.866e-629.79G protein-coupled receptor 35 [Source:MGI Symbol;A... [more]
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BLAST of GCR079 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q9VZW5|FMAR_DROME4.469e-732.03FMRFamide receptor OS=Drosophila melanogaster OX=7... [more]
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BLAST of GCR079 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUK60.000e+0100.00GCR079 OS=Schmidtea mediterranea OX=79327 GN=gcr07... [more]
A0A504YH161.211e-4933.99Rhodopsin orphan GPCR (Fragment) OS=Fasciola gigan... [more]
A0A4E0RIU32.765e-4834.12Rhodopsin orphan GPCR OS=Fasciola hepatica OX=6192... [more]
A0A2H1CT953.499e-4834.127 transmembrane receptor OS=Fasciola hepatica OX=6... [more]
A0A3P7L2X21.186e-4732.76G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of GCR079 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000031230.11.474e-832.28pep primary_assembly:Astyanax_mexicanus-2.0:8:1566... [more]
lpar6b9.108e-728.95lysophosphatidic acid receptor 6b [Source:ZFIN;Acc... [more]
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BLAST of GCR079 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR079 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR079 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR079 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
lpar6b1.147e-727.13lysophosphatidic acid receptor 6-like [Source:NCBI... [more]
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BLAST of GCR079 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014330.10.000e+0100.00SMESG000014330.1[more]
SMESG000007702.18.256e-5736.68SMESG000007702.1[more]
SMESG000031888.15.931e-4531.51SMESG000031888.1[more]
SMESG000018637.13.110e-4434.71SMESG000018637.1[more]
SMESG000018637.13.411e-4434.62SMESG000018637.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004403 ID=SMED30004403|Name=GCR079|organism=Schmidtea mediterranea sexual|type=transcript|length=1579bp
TCCGATCTCAACAGGATAACAAGAACTATTCCTTGATGAAAGAAGTTCCT
TGTAGAGATTTGTTTGAATAATATTCCAAGTTTCAAAAATGAAGTTTTCT
CCAAAAATGTTATAATGCAAAATGAAGTGTAGCTATAATAATCAGTGTTT
TGATCATGGAAAGTGAAATAAACCGAACCGAAAATTTACAAAACAATTTA
ACTTTATTTCTTATACGAAAGTATTTATGGAGTTCTACGTTCTATTTGCA
TCGCTATGGTTCTTTGCTTTGGTTGATGAGTGGACTTGTATTAAACACTT
TAGCGTTTATAATATGGACCAGACCAAGACTTAGAAGATCAAATACATCC
GCAAATTATTTGGCAGGAATAGCCATGTCAGACACCATATTTTGGTTATT
ATACATTCTTCATTACATGCAAACTTCGTGGGACATTTGTGTTCAAGATA
TTCCAGTATGGTGTGAAATGTTTCAAGGTTTATTTATTTCAAGTCAATAC
TTTTCCGTACCTCTCGTTTTGATTTTCTCATTGGAGAGATATATAGCAGT
ATGCCATCCATTTAAAAGAGAAAAGCTCTGTACTCACAGAAAGGCTCTGG
TCAATACAATTGTTTGTGGAGTCACTGCGCTGATATTAGGATGTTCTCAG
AGTATTTCAAGAGAATACAATGACGTATCGAAAAAATGTGATATTAGAGG
AAGTGTTGTAAATACTCGCAATTGGTTCATATTTGCCACTGGGACTGAAA
TACTCATTAGTGCGCTGCTGCCATTTGCAACTTTAATCGTCAATATCTTA
GTTATTATAGAACTGAAATCTATCACAAGTAAAACTACATTGATAAAACC
CTCAGTAACTGCTCCATTAACCTCCTCCACTAGTACCAGTGAGCAACATC
TTCAAATTCAAAGAAACCAAGGTAGTACGGAAAAGCTAAATATCAAAAAG
CATTCCATTAGCCAACAGAATAAAGAAAAGAGTGCATTCAAATCCAGTAC
AATTTTGCTTTTAAGTGTCTCGTTTCTTCTAATAGTCACAACTCTACCAA
CCGGTTTCGTTTATGCGCTCCAATTTGTCTGTCCAGTTGGAAACGAAACT
TTAAGCGTCGAACAAATGAAAGTCGATCCAGATTGGATAAAGTTTATTAA
CTATCAAATTTTAAAAGAGGTTGTTGACTTTTTTTCAATGATCCATTTCA
GTATAAATTTTGTTATTTATTATATTACTGGCGAAAGTTTTAGAACAGAA
TTTTACCTTCTGTTTCAAAATGCAAAACGATTCAAAGGTCCTGACGATAC
TTCCATTGGTAATTCCTATTTTTCAAATAGAAATGGGCACAATCAGAGAG
TTAATCACACCGAAATGCTCCGAAAACAGGCCGAACACACGACAATTACA
AATATTTAATAAAATCCTATTTTAATTTCCTTAATTTTGATATATTATTC
GAAATTGCAGATTTCATTTATTGTGCTGCGATTGTAATTCAAAGATTTAT
TTTCTAACGTCAACATTCACCATCTCTTTCTCTCTGAGTATTGCAGAGGT
ATGTTTTATGTAAATAAACAGATCGGAAG
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protein sequence of SMED30004403-orf-1

>SMED30004403-orf-1 ID=SMED30004403-orf-1|Name=SMED30004403-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=418bp
MESEINRTENLQNNLTLFLIRKYLWSSTFYLHRYGSLLWLMSGLVLNTLA
FIIWTRPRLRRSNTSANYLAGIAMSDTIFWLLYILHYMQTSWDICVQDIP
VWCEMFQGLFISSQYFSVPLVLIFSLERYIAVCHPFKREKLCTHRKALVN
TIVCGVTALILGCSQSISREYNDVSKKCDIRGSVVNTRNWFIFATGTEIL
ISALLPFATLIVNILVIIELKSITSKTTLIKPSVTAPLTSSTSTSEQHLQ
IQRNQGSTEKLNIKKHSISQQNKEKSAFKSSTILLLSVSFLLIVTTLPTG
FVYALQFVCPVGNETLSVEQMKVDPDWIKFINYQILKEVVDFFSMIHFSI
NFVIYYITGESFRTEFYLLFQNAKRFKGPDDTSIGNSYFSNRNGHNQRVN
HTEMLRKQAEHTTITNI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 110..132
score: 24.36
coord: 65..86
score: 18.51
coord: 337..363
score: 22.6
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 50..310
e-value: 4.4E-15
score: 55.7
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 116..132
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 30..370
e-value: 8.2E-37
score: 128.9
NoneNo IPR availablePANTHERPTHR24243G-PROTEIN COUPLED RECEPTORcoord: 35..413
NoneNo IPR availablePANTHERPTHR24243:SF208ETHR, ISOFORM Acoord: 35..413
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 30..366
e-value: 5.48711E-30
score: 115.808
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 27..387
NoneNo IPR availableTMHMMTMhelixcoord: 283..305
NoneNo IPR availableTMHMMTMhelixcoord: 35..54
NoneNo IPR availableTMHMMTMhelixcoord: 147..169
NoneNo IPR availableTMHMMTMhelixcoord: 67..89
NoneNo IPR availableTMHMMTMhelixcoord: 199..218
NoneNo IPR availableTMHMMTMhelixcoord: 104..126
NoneNo IPR availableTMHMMTMhelixcoord: 339..358
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 46..355
score: 19.108