Dimer_Tnp_hAT domain-containing protein

Overview


Name	Dimer_Tnp_hAT domain-containing protein
Smed ID	SMED30004402
Length (bp)	1679

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Dimer_Tnp_hAT domain-containing protein (SMED30004402) t-SNE clustered cells

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30004402) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dimer_Tnp_hAT domain-containing protein (SMED30004402) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30004402) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30004402

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
pharynx	SMED30004402	dd_Smed_v4_14487_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30004402	dd_Smed_v4_14487_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
dorsal region of the epidermis	SMED30004402	dd_Smed_v4_14487_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Match: MED25 (mediator complex subunit 25 [Source:HGNC Symbol;Acc:HGNC:28845])

HSP 1 Score: 54.299 bits (129), Expect = 5.297e-7
Identity = 26/71 (36.62%), Postives = 39/71 (54.93%), Query Frame = 2

Query: 1223 IVSRRGIVTKKRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYV 1417
            +  R  +   KR+ E E R F+ +WE   FF+++K      IC+  +S  K +N+KRHYE KH +  D Y 
Sbjct:  708 LPPRAPLPAAKRKREGEGRVFREKWERAYFFVEVKSMPMCLICKQIVSVLKEYNLKRHYESKHSKSYDQYT 778

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Match: GTF2IRD2 (GTF2I repeat domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30775])

HSP 1 Score: 52.7582 bits (125), Expect = 1.916e-6
Identity = 29/76 (38.16%), Postives = 44/76 (57.89%), Query Frame = 2

Query: 1253 KRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG-*RKVKLQLLKANL 1459
            KR+I+ E R FQ +WE   FF++++      IC+ S+S  K +N++RHY+  H +  D Y+   R  KL  LK  L
Sbjct:  580 KRKIDQEGRVFQEKWERAYFFVEVQNIPTCLICKQSMSVSKEYNLRRHYQTNHSKHYDQYMERMRDEKLHELKKGL 655

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Match: GTF2IRD2B (GTF2I repeat domain containing 2B [Source:HGNC Symbol;Acc:HGNC:33125])

HSP 1 Score: 52.373 bits (124), Expect = 2.438e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = 2

Query: 1253 KRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG-*RKVKLQLLKANL 1459
            KR+I+ E R FQ +WE   FF++++      IC+ S+S  K +N++RHY+  H +  D Y    R  KL  LK  L
Sbjct:  585 KRKIDQEGRVFQEKWERAYFFVEVQNIPTCLICKQSMSVSKEYNLRRHYQTNHSKHYDQYTERMRDEKLHELKKGL 660

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Zebrafish
Match: AL928808.1 (pep chromosome:GRCz11:20:17257101:17259573:-1 gene:ENSDARG00000101333.2 transcript:ENSDART00000166397.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL928808.1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.515e-10
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 2

Query: 1226 VSRRGIVTKKRRIEDENRTFQSEWEDL*FFIQ------IKICRDSISTFKLHNIKRHYEQKHGEIEDIYVG---*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NP 1561
            ++  G  T KR+ EDE RTF SEWEDL FF++        IC+ S+S FK  N++RH+   H  +   +      RK K++ LK     Q  +F K +  +  +  AS ++ + IA    P
Sbjct:    1 MAESGKRTAKRKYEDERRTFLSEWEDLYFFVERNGKPFCLICQTSLSHFKASNLERHFTSLHSAVAREFPKGSELRKHKVKTLKGQAEKQTQLFRKFSKHSETVTLASYQLAWNIARAKKP 121

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Match: spag1 (sperm associated antigen 1 [Source:Xenbase;Acc:XB-GENE-853609])

HSP 1 Score: 69.3218 bits (168), Expect = 8.362e-12
Identity = 42/116 (36.21%), Postives = 61/116 (52.59%), Query Frame = 2

Query: 1244 VTKKRRIEDENRTFQSEWEDL*FF------IQIKICRDSISTFKLHNIKRHYEQKHGEIEDIYVG*RKVK-LQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLST 1570
              KKR+I+ ENR FQ +WE+  FF       Q  +C  +IS+ K +N+KRHY   HG     Y G  ++  ++ LK+ L  Q+NIF     T  A + AS  +  +IA    P S+
Sbjct:   14 CAKKRKIDSENREFQEKWEEEYFFGKNKDKPQCLVCAQTISSCKEYNVKRHYTTLHGAKYAKYTGEARLTIIKDLKSKLCKQKNIFSAVTTTQKAAVKASFLVAEEIAKRKYPFSS 129

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000016563.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:37379994:37381838:-1 gene:ENSXETG00000009412.1 transcript:ENSXETT00000016563.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 8.473e-12
Identity = 42/116 (36.21%), Postives = 61/116 (52.59%), Query Frame = 2

Query: 1244 VTKKRRIEDENRTFQSEWEDL*FF------IQIKICRDSISTFKLHNIKRHYEQKHGEIEDIYVG*RKVK-LQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLST 1570
              KKR+I+ ENR FQ +WE+  FF       Q  +C  +IS+ K +N+KRHY   HG     Y G  ++  ++ LK+ L  Q+NIF     T  A + AS  +  +IA    P S+
Sbjct:   14 CAKKRKIDSENREFQEKWEEEYFFGKNKDKPQCLVCAQTISSCKEYNVKRHYTTLHGAKYAKYTGEARLTIIKDLKSKLCKQKNIFSAVTTTQKAAVKASFLVAEEIAKRKYPFSS 129

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
Match: Med25 (mediator complex subunit 25 [Source:MGI Symbol;Acc:MGI:1922863])

HSP 1 Score: 53.1434 bits (126), Expect = 8.045e-7
Identity = 25/71 (35.21%), Postives = 40/71 (56.34%), Query Frame = 2

Query: 1223 IVSRRGIVTKKRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYV 1417
            +  R  +   KR+ E E R F+ +WE   FF+++K      IC+ ++S  K +N+KRHYE +H +  D Y 
Sbjct:  706 LPQRAPLPVAKRKREGEGRVFREKWERDYFFVEVKSMPTCLICKKNVSVLKEYNLKRHYESQHSKSYDQYT 776

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
Match: Gtf2ird2 (GTF2I repeat domain containing 2 [Source:MGI Symbol;Acc:MGI:2149780])

HSP 1 Score: 51.6026 bits (122), Expect = 2.618e-6
Identity = 30/76 (39.47%), Postives = 45/76 (59.21%), Query Frame = 2

Query: 1253 KRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG-*RKVKLQLLKANL 1459
            KR+I+ E R FQ +WE   FF++++      IC+ S+S  K +N++RHY+  H    D Y G  R+ KL+ LK  L
Sbjct:  414 KRKIDQEGRVFQEKWERAYFFVEVQNIPTCLICKQSVSVSKEYNLRRHYQTNHSRHYDQYSGQAREEKLRELKRGL 489

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. UniProt/SwissProt
Match: sp|A4IFA3|GT2D2_BOVIN (General transcription factor II-I repeat domain-containing protein 2 OS=Bos taurus OX=9913 GN=GTF2IRD2 PE=2 SV=1)

HSP 1 Score: 53.5286 bits (127), Expect = 4.986e-6
Identity = 36/106 (33.96%), Postives = 53/106 (50.00%), Query Frame = 2

Query: 1253 KRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYV-G*RKVKLQLLKANL------------TTQQNIFHKQNVTTNAII 1513
            KR+I+ E R FQ +WE   FF++++      IC+ S+S  K +N++RHY+  H +  D Y    R  KLQ LK  L              Q+ +F K N   NA +
Sbjct:  419 KRKIDQEGRVFQEKWERAYFFVEVQNIPTCLICKQSMSVSKEYNLRRHYQTNHSKHYDQYTEKMRDEKLQELKEGLRKYLLGSSDTVCPEQKQVFAKVNPRENAAV 524

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A5E4M951 (Uncharacterized protein OS=Cinara cedri OX=506608 GN=CINCED_3A006216 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.861e-12
Identity = 43/114 (37.72%), Postives = 66/114 (57.89%), Query Frame = 2

Query: 1247 TKKRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHG-EIEDIYVG*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            +KKR+I+ E R FQ +W +  F I+ K      +CR+ IS  K +NIKRHYE KH  + + +Y   R++++  L+  LT +Q IF K  +   A+ISAS+ +   IA    P +
Sbjct:    4 SKKRKIDSECRVFQHKWSNQYFVIENKGKVMCLVCRELISVLKEYNIKRHYESKHKVKYDSLYGQFREIEVNKLQKALTGEQTIFSKVTIQNKAVISASVNVAMLIAKEGKPFT 117

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: D6WTP3 (General transcription factor II-I repeat domain-containing protein 2-like Protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC010214 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.793e-11
Identity = 40/114 (35.09%), Postives = 65/114 (57.02%), Query Frame = 2

Query: 1247 TKKRRIEDENRTFQSEWEDL*FFIQ------IKICRDSISTFKLHNIKRHYEQKHGEIEDIYVG-*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            TKKR+++ E R FQ +W ++ FF+         IC++S+S FK HNIKRHY+ KH +  + YVG  R+ K+  L++ +     +F+       +I+ AS R+ + I    +P S
Sbjct:    3 TKKRKVDSECRVFQEKWTNMYFFVAQGEKCLCLICKESLSVFKEHNIKRHYDSKHKQTYNKYVGVCRENKITNLQSEIGGLTKLFNNAKNINESIVRASYRVGHIIGKEVHPFS 116

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: J9JJ25 (DUF4371 domain-containing protein OS=Acyrthosiphon pisum OX=7029 GN=103308085 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.264e-11
Identity = 44/115 (38.26%), Postives = 65/115 (56.52%), Query Frame = 2

Query: 1244 VTKKRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG*RKV-KLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            V+KKR+I DE+R FQ +W +  FFIQ+K      IC++SI+  K +N+KRHY  KH    D+  G  ++ KL LL  N+  Q +   K +  + A + AS  I  KI+    P +
Sbjct:    3 VSKKRKISDESRVFQEKWSNNYFFIQVKEKAICLICQESIAVMKEYNLKRHYGTKHAAKYDMIQGQLRIDKLALLMKNIQGQSSGLKKYHKDSEASVKASFIIAQKISAKSKPFT 117

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A2S2Q713 (General transcription factor II-I repeat domain-containing protein 2 OS=Sipha flava OX=143950 GN=Gtf2ird2_4 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.379e-11
Identity = 39/115 (33.91%), Postives = 66/115 (57.39%), Query Frame = 2

Query: 1244 VTKKRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKH-GEIEDIYVG*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            +  KR+I DE+R F+ +W +L FFI++       IC  +I+  K +NI+RHY+ +H  + E++    R+ K++    +L++QQNIF  QN    A + AS  +   +A N  P +
Sbjct:    1 MAPKRKINDEHRIFKEDWTELFFFIELGGKLLCLICNKTITVMKEYNIRRHYDNEHKAKFENLTGELRQNKIKHFLTSLSSQQNIFKSQNTRNEASVRASYVVAEMLAKNNRPFT 115

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: X1X043 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.747e-10
Identity = 44/115 (38.26%), Postives = 64/115 (55.65%), Query Frame = 2

Query: 1244 VTKKRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG-*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            ++KKR+I DE+R F   W DL FFI+ K      IC+ +I T K +N+KRHYE +H    D   G  R++K+   K++LT QQN F  Q+    + + AS  +   IA    P +
Sbjct:    1 MSKKRKISDEHRIFNESWTDLYFFIENKGKLLCLICKKTILTVKEYNVKRHYETEHKLKFDTLTGDLRQIKINNFKSSLTNQQNSFKLQHNQNESGVRASFVVAEMIAKCNRPFN 115

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000052542.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000549.1:35642:40620:1 gene:ENSAMXG00000033785.1 transcript:ENSAMXT00000052542.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 67.3958 bits (163), Expect = 1.581e-11
Identity = 37/113 (32.74%), Postives = 61/113 (53.98%), Query Frame = 2

Query: 1247 TKKRRIEDENRTFQSEWEDL*FFIQIK-------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPL 1564
            +KKR+++ E+R F  EW    FF +         +C + ++  K +N++RHYE KH ++ D+    RK K++ LK  L +QQ +F K+   + A   AS RI   +A +  P 
Sbjct:    4 SKKRKVDLEHRKFNPEWTSKYFFYETSSAVFSCLVCLECVAVAKEYNLRRHYETKHPKLADLQGEDRKQKIEALKKQLVSQQGVFEKRRKESEACTIASYRIANLVAKSARPF 116

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000030525.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:12992682:12994541:1 gene:ENSAMXG00000035573.1 transcript:ENSAMXT00000030525.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.151 bits (139), Expect = 1.791e-8
Identity = 37/112 (33.04%), Postives = 57/112 (50.89%), Query Frame = 2

Query: 1253 KRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG*RKV-KLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            KR+I DE+R FQ +WE   FF++ +      IC + ++  K +N+KRHY  +H E    Y G  +  ++  LK  L  QQ+ F K     +A + AS  +   IA    P +
Sbjct:   61 KRKICDEHRQFQEKWEVQFFFVEHRGIPTCLICTEKVAVHKEYNLKRHYTTRHAEEYAKYQGDERANRVANLKTCLLRQQDFFKKATKENDAAVEASYVVSEMIAKAGKPFT 172

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000048309.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000491.1:94927:99076:1 gene:ENSAMXG00000033081.1 transcript:ENSAMXT00000048309.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 1.627e-7
Identity = 33/110 (30.00%), Postives = 57/110 (51.82%), Query Frame = 2

Query: 1259 RIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG-*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            +++ ENR FQ++WE+   F + K      +C +++S  K +N+ RHY   H    + Y G  R   +  LK+ +  QQ+ F K   +  + I+AS  +  ++A    PLS
Sbjct:   36 KLDRENRIFQTKWEEEYLFTEFKGKPMCLVCLETLSALKDYNLSRHYSTLHKVKYEKYTGAARAAVVADLKSKVGKQQHFFTKATTSQESAITASYDVALELARAKKPLS 145

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000037725.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:29162452:29166863:1 gene:ENSAMXG00000037303.1 transcript:ENSAMXT00000037725.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 2.016e-7
Identity = 27/79 (34.18%), Postives = 45/79 (56.96%), Query Frame = 2

Query: 1328 ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPL 1564
            +C + ++  K +N++RHYE KH ++ D+    RK K++ LK  L +QQ +F K+   + A   AS RI   +A +  P 
Sbjct:  226 VCLECVAVAKEYNLRRHYETKHPKLADLQGEDRKQKIEALKKQLVSQQGVFEKRRKESEACTIASYRIANLVAKSARPF 304

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000035253.1 (general transcription factor II-I repeat domain-containing protein 2B-like [Source:NCBI gene;Acc:111191579])

HSP 1 Score: 53.5286 bits (127), Expect = 4.767e-7
Identity = 31/108 (28.70%), Postives = 58/108 (53.70%), Query Frame = 2

Query: 1244 VTKKRRIEDENRTFQSEWEDL*FFIQ------IKICRDSISTFKLHNIKRHYEQKHG-EIEDIYVG*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIA 1546
            +++KR+++ + R FQ  WE    F+         +C +++S  K +N++RH++ KHG +     +  ++   Q LK  L +QQN+F K     +A + AS  +  +IA
Sbjct:    1 MSRKRKVDADGRLFQQRWEGEYMFVLQGERPVCLLCCEAVSVIKEYNLRRHFDTKHGAKYGKASLQEKQQIAQELKDKLKSQQNLFTKATAKNSAAVKASFIVAEEIA 108

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000033014.1 (general transcription factor II-I repeat domain-containing protein 2 [Source:NCBI gene;Acc:101164918])

HSP 1 Score: 65.855 bits (159), Expect = 5.570e-11
Identity = 38/112 (33.93%), Postives = 63/112 (56.25%), Query Frame = 2

Query: 1250 KKRRIEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIEDIYVG*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            KKR+++ E R FQ  W+   FF  I+      IC+D+++ FK  NI+RHY+ KH   +++    R+ KL+ L++ LTTQQ+I   ++   +    AS  +   IA +  P +
Sbjct:    6 KKRKVDYEGRRFQDRWKLAYFFTDIQNNCVCLICQDTVAVFKEFNIRRHYQTKHASYDNLTGVQRREKLEQLESALTTQQSICAIKSEPDDNTTQASYEVATLIAKHCKPFT 117

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000017252.2 (pep primary_assembly:ASM223467v1:13:14820116:14821987:-1 gene:ENSORLG00000013757.2 transcript:ENSORLT00000017252.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 4.127e-7
Identity = 25/65 (38.46%), Postives = 39/65 (60.00%), Query Frame = 2

Query: 1247 TKKRRIEDENRTFQSEWEDL*FFIQIK--------ICRDSISTFKLHNIKRHYEQKHGEIEDIYV 1417
            +K ++++ ENRTF+ EW D   FI           IC ++++  K  N+KRHYE KHG  E++Y+
Sbjct:    4 SKMKKVDAENRTFKDEWTDKYMFILPAASSKPLCLICCENVALIKSGNVKRHYETKHGSFEEMYL 68

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000037770.1 (SMESG000037770.1)

HSP 1 Score: 61.6178 bits (148), Expect = 1.246e-9
Identity = 40/109 (36.70%), Postives = 58/109 (53.21%), Query Frame = 2

Query: 1262 IEDENRTFQSEWEDL*FFIQIK------ICRDSISTFKLHNIKRHYEQKHGEIE-DIYVG*RKVKLQLLKANLTTQQNIFHKQNVTTNAIISASLRILYKIATN*NPLS 1567
            IE+E RTF  +W    FF+ IK      ICR+S++ FK +N+KRH++ KHG    ++     + K   L  +L  +Q IF K +    A   ASL I +KI  +  P S
Sbjct:  529 IEEECRTFNKDWTSKYFFVDIKTNAVCLICRESVAVFKEYNLKRHFQTKHGNFGINLNETELRRKADDLVRSLKQEQTIFLKPSSIQEAATKASLVIAHKIVKHNKPFS 637

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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000045419.1 (SMESG000045419.1)

HSP 1 Score: 58.5362 bits (140), Expect = 8.252e-9
Identity = 36/93 (38.71%), Postives = 54/93 (58.06%), Query Frame = 2

Query: 1241 IVTKKRRIEDENRTFQSEWEDL*FFIQ------IKICRDSISTFKLHNIKRHYEQKHG-EIEDIYVG*RKVKLQLLKANLTTQQNIFHKQNVT 1498
            + ++KR+I DE R F  EW    FF +        I   S++  K +N+KRHYE++H  + +D+    RK K + LKA+LT QQ+IF K+  T
Sbjct:    1 MSSRKRKIADEGRVFNEEWNSKYFFTENGGKPLCLIFNKSVAVMKEYNVKRHYEKEHERQYKDLTGEIRKNKFRTLKASLTAQQSIFMKRPFT 93

The following BLAST results are available for this feature:

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Celegans
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Fly
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Zebrafish
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
BLAST of Dimer_Tnp_hAT domain-containing protein vs. UniProt/SwissProt
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Sea Lamprey
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Yeast
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Nematostella
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
MED25	5.297e-7	36.62	mediator complex subunit 25 [Source:HGNC Symbol;Ac...	[more]
GTF2IRD2	1.916e-6	38.16	GTF2I repeat domain containing 2 [Source:HGNC Symb...	[more]
GTF2IRD2B	2.438e-6	38.16	GTF2I repeat domain containing 2B [Source:HGNC Sym...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
AL928808.1	1.515e-10	33.88	pep chromosome:GRCz11:20:17257101:17259573:-1 gene...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
spag1	8.362e-12	36.21	sperm associated antigen 1 [Source:Xenbase;Acc:XB-...	[more]
ENSXETT00000016563.1	8.473e-12	36.21	pep primary_assembly:Xenopus_tropicalis_v9.1:4:373...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Med25	8.045e-7	35.21	mediator complex subunit 25 [Source:MGI Symbol;Acc...	[more]
Gtf2ird2	2.618e-6	39.47	GTF2I repeat domain containing 2 [Source:MGI Symbo...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1


Match Name	E-value	Identity	Description
sp\|A4IFA3\|GT2D2_BOVIN	4.986e-6	33.96	General transcription factor II-I repeat domain-co...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A5E4M951	7.861e-12	37.72	Uncharacterized protein OS=Cinara cedri OX=506608 ...	[more]
D6WTP3	3.793e-11	35.09	General transcription factor II-I repeat domain-co...	[more]
J9JJ25	5.264e-11	38.26	DUF4371 domain-containing protein OS=Acyrthosiphon...	[more]
A0A2S2Q713	8.379e-11	33.91	General transcription factor II-I repeat domain-co...	[more]
X1X043	1.747e-10	38.26	Uncharacterized protein OS=Acyrthosiphon pisum OX=...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000052542.1	1.581e-11	32.74	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000030525.1	1.791e-8	33.04	pep primary_assembly:Astyanax_mexicanus-2.0:1:1299...	[more]
ENSAMXT00000048309.1	1.627e-7	30.00	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000037725.1	2.016e-7	34.18	pep primary_assembly:Astyanax_mexicanus-2.0:14:291...	[more]
ENSAMXT00000035253.1	4.767e-7	28.70	general transcription factor II-I repeat domain-co...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSORLT00000033014.1	5.570e-11	33.93	general transcription factor II-I repeat domain-co...	[more]
ENSORLT00000017252.2	4.127e-7	38.46	pep primary_assembly:ASM223467v1:13:14820116:14821...	[more]

BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000037770.1	1.246e-9	36.70	SMESG000037770.1	[more]
SMESG000045419.1	8.252e-9	38.71	SMESG000045419.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30004402 ID=SMED30004402|Name=Dimer_Tnp_hAT domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1679bp
AAATTTTCTTTTGAATAAAACAAAAATAATTTAATAAATGTTATGAGACA
GACATGAGTAAATATTTCGTTTATTCTCTCAAGTCAAAGAAATAATAATT
CATTTTAATTATGTTTGAAATATAAATTATAAAAATTAAAGTTAATTTTT
AAGGTGTCAACGATTTTACAATTTTATATTTCAATTTTAGTTAATTACTA
TGATTATTATTATTTATACTTAAAAGTATTATTTGCCAATGCGGGATCAT
TCATATTCAACAAGTCTTTCACATTCTGGTTCTTATCTGCTTAACCTTTC
CTTCCCACTACTGTTTTCAATAAATGACAAGATTAAAACAGTTTGTTTTA
ATAATTAAAATTTTAATTTGCCAATAACCAAATATGAATAAATCCGAATA
AAATACTTTTTAACACAATTAAATACACTTTTAAATATTTGTAAATCAAA
CTTTTAAGATTTGCTAAATTTGAATTTTTGAAAATATTTCTGTGTGGGGC
TGATTTATATAAACTTAAAAATATTTAGGTAGTTTTTCTACCTTTTCAAA
TAGCTAAAATTTTCATGTTATAGGCATATTAATAAAACAAGATTAAATCT
TTATAAATTATTTATTTGAATTTGTTCTTAAAAACCACATTGCTTTTATT
AAATTTATATTTGTAGAAGCTCTCAAATCAGAATTGAGGATTTTAAGAGC
GTAAAATAAGCGATCTATGGATACTTGGGTTGATGGCAATGTTGTTATGA
TCAACCCTGAGTCTTCTACGATTTCTGGATACCACTGTATTGTCATTTTT
AACATTTAAATCAGCCCTGTCCAACACGCGGCCCGCGGGTAGCATGCGGC
CCAGTTTAAGATTTTCTCCGGCCCTATTGGAGTTCGCAACTTACTATTAA
CATATTTTGTTATTCATAAATCAAACAAAACAAGTAAAAATAATTCACGC
TCGAGTGGTGCACTCGCACACCTTTCCAGTGACTAGTGTAAAAACCTCAA
TCTAACTCACTCGCGCGAAAGAACGGGATGCGGCTAATTTCATGTGAATT
TGACATCAAAATATTTATACATATTATTTACTAGAATAAGTCACATTTAG
TACAGTCTGGTCGAGTTGTAACTTCTTTGTGATATTTAGAGGCGCTTGTT
TATTAAATGAAGTAAGATAGTACAACTTTACAATTATTTAATCATATCAC
TTATAATTAAAAAAACAGATTTATCGTATCTAGAAGAGGAATTGTAACGA
AGAAAAGAAGGATTGAAGATGAGAACAGAACATTCCAGAGTGAATGGGAA
GATTTGTAATTTTTCATCCAAATAAAAATTTGTCGTGATTCTATTTCAAC
TTTCAAATTACACAACATTAAAAGACATTATGAACAGAAACATGGTGAAA
TAGAAGACATTTATGTTGGCTAACGAAAGGTTAAGTTACAATTGCTAAAG
GCCAATCTTACAACACAACAAAACATATTTCATAAGCAAAATGTAACAAC
AAACGCTATTATCAGTGCCAGCCTGCGAATACTATATAAAATTGCTACAA
ATTGAAACCCTTTATCAACGAAATGAAATAAGTCCGCTAACATATCGTAA
GCATTTTAGTTCATTATTATTGAAAAAGAAATTTCATAAATGACAAACAC
TGAACTGCTTATTTTAATAAAAAACTTAA

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0046983	protein dimerization activity
GO:0003676	nucleic acid binding

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0000054	dorsal region of the epidermis

Main menu

Dimer_Tnp_hAT domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

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