Alpha-2-macroglobulin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004348 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 25Note: Hover over icons to view figure legend
Homology
BLAST of Alpha-2-macroglobulin vs. Ensembl Human
Match: A2ML1 (alpha-2-macroglobulin like 1 [Source:HGNC Symbol;Acc:HGNC:23336]) HSP 1 Score: 338.961 bits (868), Expect = 5.754e-97 Identity = 280/976 (28.69%), Postives = 456/976 (46.72%), Query Frame = 1
Query: 2059 MGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVD--PGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLL------------RKHKC-------IPQKRVKRMIQ-----------PIYGLEYDSRKVLSTAGFTAITNARIVN-VKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSF-----TLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYW---PFDKESSDEQKSNSA-DIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGV-SQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAAN-DTFKLSV---KTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPL-NRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+ PD ++ Y I VVAD ++ + + +Q+ G F+ +P ++V L + PG + CA+R DE++L L++ + +++ S G+ +C PQ + M Q P + D G ++NA+I V C + P+ Y+T ++ S + A+ Q+R+YFPE+W++ F N + +++TVPD++T W+A S+C ++ +G ++PT L PF+ ++ +PYS+ E +L +FNY C +++ D + + L+S+ + C C +D KT ++N+ +G +N TI + S+ +K + SD++ + +LV+P GV E T S L+C K + +P +IV S ++Y+T GD+MG NLD LV MP GCGEQNMV P IYVL YL + + ++A ++ GY ++L YKH +GS+SAFG+ D G+TWLTAFV K F A K++ I+ +++A K++ Q GC+ G L + M GG+ D LTAYV +L G D+ + +N +T N + + + QL Q++ G YW P ++ +A D+E T YA+LA + ++++ + V WL+ Q N+ GG+ STQDTVVAL ALA +A T + S+ +NL V N +T+N I NRLV Q L Y +++ +G GC +Q YNI L N TF LSV K + + + +Y+ +SNM +V + SG+ E + L Q PL +++F + +Y D N + F I + ++NL+PA +K+ DYY P+ + YS
Sbjct: 37 LAPDPSLVIYAIFPSGGVVADKIQFSVEMCFDNQVSLG---FSPSQQLPGAEVELQLQAAPG---SLCALRAVDESVL----------------LLRPDRELSNRSVYGMFPFWYGHYPYQVAEYDQCPVSGPWDFPQPLIDPMPQGHSSQRSIIWRPSFSEGTDLFSFFRDVGLKILSNAKIKKPVDC----SHRSPE--YSTAMGAGGGHPEAFESSTPLHQAE-DSQVRQYFPETWLWDLFPIGNSGKEAVHVTVPDAITEWKAMSFCTSQSRGFGLSPTVGLTAFKPFFVDLTLPYSVVRGESFRLTATIFNYLKDC----IRVQTDLAKSHEYQLESWADSQTSSCLCADDAKTHHWNITAVKLGHINFTISTKILDSNEPCGGQKGFVPQKGRSDTLIKPVLVKPEGVLVEKTHSSLLCPKGKVASESVSLELPVDIVPDSTKAYVTVLGDIMGTALQNLDGLVQMPSGCGEQNMVLFAPIIYVLQYLEKAGLLTEEIRSRAVGFLEIGYQKELMYKHSNGSYSAFGERDGNGNTWLTAFVTKCFGQAQKFIFIDPKNIQDALKWMAGNQLPSGCYANVGNLLHTAMKGGVD-----DEVSLTAYVTAALLEMGKDV---DDPMVSQGLRCLKNSATSTTNLYTQALLAYIFSLAGEMDIRNILLKQLDQQAIISGESIYWSQKPTPSSNASPWSEPAAVDVELTAYALLAQLTKPSLTQKEIAKATSIVAWLAKQHNAYGGFSSTQDTVVALQALAKYATTAYMPSEEINLVVKSTENFQRTFN--IQSVNRLVFQQDTLPNVPGMY-TLEASGQGCVYVQTVLRYNI--LPPTNMKTFSLSVEIGKARCEQPTSPRSLTLTIHTSYVGSRSSSNMAIVEVKMLSGFSPMEGTNQLLLQQ---PLVKKVEFGTDTLNIYLDELIKN----TQTYTFTISQSVLVTNLKPATIKVYDYYLPDEQATIQYS 959
BLAST of Alpha-2-macroglobulin vs. Ensembl Human
Match: A2ML1 (alpha-2-macroglobulin like 1 [Source:HGNC Symbol;Acc:HGNC:23336]) HSP 1 Score: 338.191 bits (866), Expect = 2.243e-96 Identity = 280/976 (28.69%), Postives = 456/976 (46.72%), Query Frame = 1
Query: 2059 MGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVD--PGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLL------------RKHKC-------IPQKRVKRMIQ-----------PIYGLEYDSRKVLSTAGFTAITNARIVN-VKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSF-----TLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYW---PFDKESSDEQKSNSA-DIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGV-SQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAAN-DTFKLSV---KTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPL-NRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+ PD ++ Y I VVAD ++ + + +Q+ G F+ +P ++V L + PG + CA+R DE++L L++ + +++ S G+ +C PQ + M Q P + D G ++NA+I V C + P+ Y+T ++ S + A+ Q+R+YFPE+W++ F N + +++TVPD++T W+A S+C ++ +G ++PT L PF+ ++ +PYS+ E +L +FNY C +++ D + + L+S+ + C C +D KT ++N+ +G +N TI + S+ +K + SD++ + +LV+P GV E T S L+C K + +P +IV S ++Y+T GD+MG NLD LV MP GCGEQNMV P IYVL YL + + ++A ++ GY ++L YKH +GS+SAFG+ D G+TWLTAFV K F A K++ I+ +++A K++ Q GC+ G L + M GG+ D LTAYV +L G D+ + +N +T N + + + QL Q++ G YW P ++ +A D+E T YA+LA + ++++ + V WL+ Q N+ GG+ STQDTVVAL ALA +A T + S+ +NL V N +T+N I NRLV Q L Y +++ +G GC +Q YNI L N TF LSV K + + + +Y+ +SNM +V + SG+ E + L Q PL +++F + +Y D N + F I + ++NL+PA +K+ DYY P+ + YS
Sbjct: 78 LAPDPSLVIYAIFPSGGVVADKIQFSVEMCFDNQVSLG---FSPSQQLPGAEVELQLQAAPG---SLCALRAVDESVL----------------LLRPDRELSNRSVYGMFPFWYGHYPYQVAEYDQCPVSGPWDFPQPLIDPMPQGHSSQRSIIWRPSFSEGTDLFSFFRDVGLKILSNAKIKKPVDC----SHRSPE--YSTAMGAGGGHPEAFESSTPLHQAE-DSQVRQYFPETWLWDLFPIGNSGKEAVHVTVPDAITEWKAMSFCTSQSRGFGLSPTVGLTAFKPFFVDLTLPYSVVRGESFRLTATIFNYLKDC----IRVQTDLAKSHEYQLESWADSQTSSCLCADDAKTHHWNITAVKLGHINFTISTKILDSNEPCGGQKGFVPQKGRSDTLIKPVLVKPEGVLVEKTHSSLLCPKGKVASESVSLELPVDIVPDSTKAYVTVLGDIMGTALQNLDGLVQMPSGCGEQNMVLFAPIIYVLQYLEKAGLLTEEIRSRAVGFLEIGYQKELMYKHSNGSYSAFGERDGNGNTWLTAFVTKCFGQAQKFIFIDPKNIQDALKWMAGNQLPSGCYANVGNLLHTAMKGGVD-----DEVSLTAYVTAALLEMGKDV---DDPMVSQGLRCLKNSATSTTNLYTQALLAYIFSLAGEMDIRNILLKQLDQQAIISGESIYWSQKPTPSSNASPWSEPAAVDVELTAYALLAQLTKPSLTQKEIAKATSIVAWLAKQHNAYGGFSSTQDTVVALQALAKYATTAYMPSEEINLVVKSTENFQRTFN--IQSVNRLVFQQDTLPNVPGMY-TLEASGQGCVYVQTVLRYNI--LPPTNMKTFSLSVEIGKARCEQPTSPRSLTLTIHTSYVGSRSSSNMAIVEVKMLSGFSPMEGTNQLLLQQ---PLVKKVEFGTDTLNIYLDELIKN----TQTYTFTISQSVLVTNLKPATIKVYDYYLPDEQATIQYS 1000
BLAST of Alpha-2-macroglobulin vs. Ensembl Human
Match: A2ML1 (alpha-2-macroglobulin like 1 [Source:HGNC Symbol;Acc:HGNC:23336]) HSP 1 Score: 339.732 bits (870), Expect = 2.092e-94 Identity = 280/984 (28.46%), Postives = 458/984 (46.54%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVD--PGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLL------------RKHKC-------IPQKRVKRMIQ-----------PIYGLEYDSRKVLSTAGFTAITNARIVN-VKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSF-----TLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPF----DKESSDEQKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGV-SQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAAN-DTFKLSV---KTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPL-NRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+S+ + PD ++ Y I VVAD ++ + + +Q+ G F+ +P ++V L + PG + CA+R DE++L L++ + +++ S G+ +C PQ + M Q P + D G ++NA+I V C + P+ Y+T ++ S + A+ Q+R+YFPE+W++ F N + +++TVPD++T W+A S+C ++ +G ++PT L PF+ ++ +PYS+ E +L +FNY C +++ D + + L+S+ + C C +D KT ++N+ +G +N TI + S+ +K + SD++ + +LV+P GV E T S L+C K + +P +IV S ++Y+T GD+MG NLD LV MP GCGEQNMV P IYVL YL + + ++A ++ GY ++L YKH +GS+SAFG+ D G+TWLTAFV K F A K++ I+ +++A K++ Q GC+ G L + M GG+ D LTAYV +L G D+ + +N +T N + + + QL Q++ G YW +S + + D+E T YA+LA + ++++ + V WL+ Q N+ GG+ STQDTVVAL ALA +A T + S+ +NL V N +T+N I NRLV Q L Y +++ +G GC +Q YNI L N TF LSV K + + + +Y+ +SNM +V + SG+ E + L Q PL +++F + +Y D N + F I + ++NL+PA +K+ DYY P+ + YS
Sbjct: 520 LSLTFTSRLAPDPSLVIYAIFPSGGVVADKIQFSVEMCFDNQVSLG---FSPSQQLPGAEVELQLQAAPG---SLCALRAVDESVL----------------LLRPDRELSNRSVYGMFPFWYGHYPYQVAEYDQCPVSGPWDFPQPLIDPMPQGHSSQRSIIWRPSFSEGTDLFSFFRDVGLKILSNAKIKKPVDC----SHRSPE--YSTAMGAGGGHPEAFESSTPLHQAE-DSQVRQYFPETWLWDLFPIGNSGKEAVHVTVPDAITEWKAMSFCTSQSRGFGLSPTVGLTAFKPFFVDLTLPYSVVRGESFRLTATIFNYLKDC----IRVQTDLAKSHEYQLESWADSQTSSCLCADDAKTHHWNITAVKLGHINFTISTKILDSNEPCGGQKGFVPQKGRSDTLIKPVLVKPEGVLVEKTHSSLLCPKGKVASESVSLELPVDIVPDSTKAYVTVLGDIMGTALQNLDGLVQMPSGCGEQNMVLFAPIIYVLQYLEKAGLLTEEIRSRAVGFLEIGYQKELMYKHSNGSYSAFGERDGNGNTWLTAFVTKCFGQAQKFIFIDPKNIQDALKWMAGNQLPSGCYANVGNLLHTAMKGGVD-----DEVSLTAYVTAALLEMGKDV---DDPMVSQGLRCLKNSATSTTNLYTQALLAYIFSLAGEMDIRNILLKQLDQQAIISGESIYWSQKPTPSSNASPWSEPAAVDVELTAYALLAQLTKPSLTQKEIAKATSIVAWLAKQHNAYGGFSSTQDTVVALQALAKYATTAYMPSEEINLVVKSTENFQRTFN--IQSVNRLVFQQDTLPNVPGMY-TLEASGQGCVYVQTVLRYNI--LPPTNMKTFSLSVEIGKARCEQPTSPRSLTLTIHTSYVGSRSSSNMAIVEVKMLSGFSPMEGTNQLLLQQ---PLVKKVEFGTDTLNIYLDELIKN----TQTYTFTISQSVLVTNLKPATIKVYDYYLPDEQATIQYS 1450 HSP 2 Score: 90.5077 bits (223), Expect = 3.259e-17 Identity = 81/322 (25.16%), Postives = 139/322 (43.17%), Query Frame = 1
Query: 433 FAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCM---LYSYGK-------KKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINV--TVTENGTSSSVSKAVEGPGIKYNSFNIKF-DGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
F +TDKP+Y PG +V R + +D N F+ ND + ++ ++ P++ R+AQW V + + +L +QL + + G + AV +G+ F +++YVLP+F VEV+ P E+ ES K+C +Y+ GKPM + C Y Y + KC K C ++ ++ ++ + QIN+ TV E GT + A + I ++ F D +Y P P+ G+I + H+ K + + I YG N F + ++ G+ F +
Sbjct: 123 FVQTDKPLYTPGQQVYFRI--------------------VTMDSN--FVPV---NDK----YSMVELQDPNSNRIAQWLEVVPEQGIVDLSFQLAPEAMLGTYTV-AVAEGKT-FGTFSVEEYVLPKFKVEVVEPKELSTVQESFLVKICCRYTYGKPMLGAVQVSVCQKANTYWYREVEREQLPDKCRNLSGQTDKTGCFSAPVDMATFDLIGYAYSH-----QINIVATVVEEGTGVE-ANATQNIYISPQMGSMTFEDTSNFYHPNFPFSGKIRVRGHDDSFLKNHLVFLVI----YGTNGTFNQTLVTDNNGLAPFTL 403
BLAST of Alpha-2-macroglobulin vs. Ensembl Human
Match: A2M (alpha-2-macroglobulin [Source:HGNC Symbol;Acc:HGNC:7]) HSP 1 Score: 334.724 bits (857), Expect = 9.057e-93 Identity = 276/984 (28.05%), Postives = 455/984 (46.24%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADAL--EIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYL-VQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEY---------DSRKVLSTAGFTAITNARIVNVK----CMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINV-IGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKT--DKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXX-----XXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSD------EQKSNSADIETTGYAILALINHQ------DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNAL-----AVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAAND-TFKLSVKT---EIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNK 4830
ISIP+ ++ P ++ Y + +V+ D+ ++ + L N L F+ ++P S L V ++ CA+R D+++L +A+LS V L+ + CI + V I G+ Y D L G A TN++I K QY G P+ F E M + ++ + + + +R+YFPE+W++ + + +TVPD++T W+A ++C++E GL I+ T L+ PF+ + +PYS+ E L V NY C ++V++ + V + + C C N +TV + + PK +G +N T+ + + S + + D+V + +LVEP G+ ETTF+ L+C ++ +P +V S R+ ++ GD++G N L+ MPYGCGEQNMV P+IYVLDYLN P + +KA Y+ GY RQL YKH DGS+S FG+ G+TWLTAFVLK+F A Y+ I+ + + +A +L + Q+ +GCF G L ++ + GG++ D L+AY+ I+L + LT T + A T ++ + + + V L +++V +W ++ E ++ SA++E T Y +LA + Q DL+ + V W++ Q+N+ GG+ STQDTVVAL+AL A F +T +Q V + + T++ +D NRL+ Q +L Y SMK G GC +Q S YNI L + F L V+T D K I + +Y SNM +V + SG+ + L+ S ++R + + +Y D S + F + + + +L+PA+VK+ DYY + E + + +N C+K
Sbjct: 526 ISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDL-----SFSPSQSLPASHAHLRVT-AAPQSVCALRAVDQSVLLM--KPDAELSASSVYNLLPEKDLTGFPGPLNDQDNEDCINRHNVY-----INGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHG-PEGLRVGFY-ESDVMGRGHARLVHVEEPHTET-VRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVE-----DEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQ-------VTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEY-SMKVTGEGCVYLQTSLKYNI--LPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGF--IPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVS----NQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETD---EFAIAEYNAPCSK 1469 HSP 2 Score: 90.5077 bits (223), Expect = 3.114e-17 Identity = 80/330 (24.24%), Postives = 154/330 (46.67%), Query Frame = 1
Query: 415 DKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKA--KAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYS-----YGKKK---CVAFKSDLMKQNCNKIKLESKIVFILASSFR---YSKAKSQINVTVTE--NGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
+++ ++F +TDK IYKPG V+ R ++ +D+N +++ L+ I+ P R+AQW++ + + + L+ +P G +K + G+ E F ++++VLP+F V+V P I +E ++ VC Y+ GKP+ ++ C YS +G+ C F L C ++++K+ + + +++A+ Q TV E SS +++ I SF +K D +++ G+P+FG++ LV + K + N I I+ GN + N T+++ G+++F I
Sbjct: 124 NEDSLVFVQTDKSIYKPGQTVKFRVVS--------------------MDENFHPLNE---------LIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEH-PFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRT-----ITKLSF-VKVDS--HFRQGIPFFGQVRLV--DGKGVPIPNKVIFIR----GNEANYYSNATTDEHGLVQFSI 409
BLAST of Alpha-2-macroglobulin vs. Ensembl Human
Match: CD109 (CD109 molecule [Source:HGNC Symbol;Acc:HGNC:21685]) HSP 1 Score: 313.153 bits (801), Expect = 3.644e-86 Identity = 276/964 (28.63%), Postives = 457/964 (47.41%), Query Frame = 1
Query: 2065 PDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKM--KKQIREYFPESWVFSYFN---KTTKHLNLTVPDSMTTWRADSYCMTEK---GLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKIN--------VDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVP---TETTFSELICNK-NEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNN-TPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYW-PFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKT-YNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAA--------NDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNK--ACNKSDEKEPGRP 4857
P VI Y+I D E+++D L+I L +++ K ++K A P KV L + ++ I D+++ S++ + +V +L N L G+ +S V G +T+A + Y+ + YA ER M ++ ++ I D + +R++FPE+W++ N + + +TVPDS+T+W A + ++E GL P + L+ PF+ + +PYS+ E L + +FNY E+ V I + +NEIN G + TL D TV F ++P +G++ +T+ ++ SD+V + ILV+ G+ +++ +L N+ L LS F+ P V+GS R +T GDV+G + + L L+ MPYGCGEQNM+ P+IY+LDYL L KA S++ GY R+L Y+ +DGSFSAFG D GSTWL+AFVL+ F +A Y++I+ + L +L+ Q+S+G F + G++ S + GG K LTAY++ SL G I++ ++I+ + + NY L +L + S ++ L ++ G ++W + + SD + S DIE YA+L+ S + + WLS QRNSLGG+ STQDT VAL AL+ FA + ++ N++V+V + + LI NRL+ QT L + V++ NG G A QL+ +YN+ ++ + F L V + ++ N ++ VC ++ + S M L+ +N SG+ S ++ +S + ++++ ++ LY D S N+ C K+SN Q A V IVDYY P + +SY+ K +C+ + + RP
Sbjct: 450 PKACVIVYYIEDDGEIISDVLKIPVQLVFKNKI---KLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYL--------------------------GMFMNSFAVFQECGLWVLTDANLTK----DYIDGVYDNAEYA----ERF-MEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVE-LQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQ-TLLVPSEDGATVLFPIRPTHLGEIPITV---------------TALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLS-FSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNK-----SPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNY--TLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMN-TERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVK-ENKDDLNHVDLNVCTSFSGPGR-SGMALMEVNLLSGF-----MVPSEAISLSETVKKVEYDHGKLNLYLD--SVNET--QFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRP 1338 HSP 2 Score: 59.6918 bits (143), Expect = 7.824e-8 Identity = 79/331 (23.87%), Postives = 136/331 (41.09%), Query Frame = 1
Query: 703 YQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSK-------AKSQINVTVTEN--GTSSSVSKAVEGPGIKYNSFNIK-FDGRPYYKPGLPYFGRINLVRHNKKPAKMV----NISISIQSWAYGNNWGFIR------------NYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSNLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKM 1617
+QL+ PI G W + + Q F++ +YVLP+F V + P + ++ + +KY+ GKP+ ++ F L +GKKK + K+ + + N + ++ ++ SS S+ +I TVTE+ G S +VS V K + + I+ FD KP L + + + R + + N+ I++ Y W NYT + G F I + L S +Q+ K MA ++ F+S Y+Q ++ K L + N + S V SRGQ+V +
Sbjct: 116 FQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNIT-KTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNV---FFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGT--FKIEFPILEDSSELQLKAYFLGSKSSMAVHSL------FKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMV--VSRGQLVAV 432
BLAST of Alpha-2-macroglobulin vs. Ensembl Celegans
Match: tep-1 (TEP (ThiolEster containing Protein) [Source:UniProtKB/TrEMBL;Acc:Q7JKM0]) HSP 1 Score: 326.25 bits (835), Expect = 2.477e-90 Identity = 273/976 (27.97%), Postives = 455/976 (46.62%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAY-FINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLE-YDSRKVLSTAGFTAITNARIVNVKCMQYLA----RGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTV--PDSMTTWRADSYCMTEK-GLWIAPT-QKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDE-----------------KTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAI--PSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENI-TNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQIST-----PLNRIDFQK--TRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPE-----IYSEKSYSMFNKACNK 4830
IS P NM P +I Y I + EV+ DAL+ +Q+ +K+ P V V K + + + D+++L ++ + Q L + + G R + I +K+ + + +P +G+ D++ + S AG +T+A + ++++ G P + A F+ + + +R++FP +W++S N T+ + + + PD++T+W A ++ + E+ GL +APT KL+V PF+ + +PY+++ E L V VFNY ++ V + D + + K T+ + DE K VYF + P IG++ V I I D+V + V+P G + +I N + P+++V GS ++ L GD+MG +N KLV MPYGCGEQNM+ +VP+I V+ YL + N L KA +I G R+LTYK D SFSAFG +DK GSTWLTAFV++SFH A +Y ++ + + A +L Q G F E+G++ M GG + G LTA+VLIS+ NG++ + K ++ + N T Y L L + + + + D + + K + + ++ DIETT YA+L+ + S S+ + WL +QRN LGG+ STQDTV+AL AL+ +A + S +V+ +LN T++ I + N +V Q++ L + N VS+ NG+G QLS Y+ +R + ND EI ++ N + +C Y R K SNM L I+ SG+ + + Q+ T L R++ +K T+M +Y++ G+ C + ++++ +PA ++VDYY+PE Y+ K + C +
Sbjct: 549 ISFPATANMAPKSRLIVYAIIESSQEVLVDALDFKVEGIFQNQVA---LSIDKQAVEPGQNVKFKVTSD-KNSFVGLLVVDQSVLLLKTGNDITREKVEQDLENYDSN-NVGGGFGGPRPWEAIDRKK-RSIWRPWWGIGGSDAQSIFSNAGLVVLTDALLYREPQREFMSVMMMDGAPGMAEAAFA-----------APPMGGSSPPPPTVRKFFPHTWIWSDLNSTSGEVEMEIEAPDTITSWVASTFAINEENGLGVAPTTSKLRVFRPFFIQLNLPYAVRRGEKFALLVLVFNYMEKEQDVTVTLKYDKDSGYDLLKKDGTVV--RRDEVGQQNVRIVSVAGGGTSKAVYFPIVPSSIGEIPVHI---------------SAIASQGGDAVEMNLRVDPQGYKVDRNIPFVIDLNNNSSDFSKNLELIWPNDVVDGSQKARLDVIGDMMGPVLNNAHKLVQMPYGCGEQNMLNLVPNILVVKYLRATNRNESQLETKAIKFIEQGIQRELTYKRADNSFSAFGDSDKAGSTWLTAFVVRSFHHAKQYAFVDPNVISRAVAFLNSQQMESGAFAERGEVHHKDMQGGAQDG----GVALTAFVLISILENGMENGKAVTYLEKHLDEVSGNAYTMAVVAYALQLAKSKQAGKAFENLKKHKIVEKSGDVKFASAQKKVEKLKESRAYMFQARPVDIETTSYAVLSYLAQNQTSESLSIIRWLVSQRNELGGFTSTQDTVMALQALSSYA-AVTYSDKHTSQVT-ILNGKHTHSFDINIRNAIVLQSYQLSSLN-DAVSINANGTGVVFAQLS--YSYYR-DSLNDDAPFFCSQEIKEIRAGNRLQLDLCCNYTRPGK-SNMALAEIDALSGY-------RFDAEQVHTLTSIEDLQRVEMEKDDTKMNVYFNPL----GGRPVCLSLYSDVTYQVADQKPANFRLVDYYDPEEQLKMTYAAKQTRSLQEKCGE 1468
BLAST of Alpha-2-macroglobulin vs. Ensembl Celegans
Match: tep-1 (TEP (ThiolEster containing Protein) [Source:UniProtKB/TrEMBL;Acc:Q7JKM0]) HSP 1 Score: 326.25 bits (835), Expect = 2.477e-90 Identity = 273/976 (27.97%), Postives = 455/976 (46.62%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAY-FINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLE-YDSRKVLSTAGFTAITNARIVNVKCMQYLA----RGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTV--PDSMTTWRADSYCMTEK-GLWIAPT-QKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDE-----------------KTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAI--PSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENI-TNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQIST-----PLNRIDFQK--TRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPE-----IYSEKSYSMFNKACNK 4830
IS P NM P +I Y I + EV+ DAL+ +Q+ +K+ P V V K + + + D+++L ++ + Q L + + G R + I +K+ + + +P +G+ D++ + S AG +T+A + ++++ G P + A F+ + + +R++FP +W++S N T+ + + + PD++T+W A ++ + E+ GL +APT KL+V PF+ + +PY+++ E L V VFNY ++ V + D + + K T+ + DE K VYF + P IG++ V I I D+V + V+P G + +I N + P+++V GS ++ L GD+MG +N KLV MPYGCGEQNM+ +VP+I V+ YL + N L KA +I G R+LTYK D SFSAFG +DK GSTWLTAFV++SFH A +Y ++ + + A +L Q G F E+G++ M GG + G LTA+VLIS+ NG++ + K ++ + N T Y L L + + + + D + + K + + ++ DIETT YA+L+ + S S+ + WL +QRN LGG+ STQDTV+AL AL+ +A + S +V+ +LN T++ I + N +V Q++ L + N VS+ NG+G QLS Y+ +R + ND EI ++ N + +C Y R K SNM L I+ SG+ + + Q+ T L R++ +K T+M +Y++ G+ C + ++++ +PA ++VDYY+PE Y+ K + C +
Sbjct: 549 ISFPATANMAPKSRLIVYAIIESSQEVLVDALDFKVEGIFQNQVA---LSIDKQAVEPGQNVKFKVTSD-KNSFVGLLVVDQSVLLLKTGNDITREKVEQDLENYDSN-NVGGGFGGPRPWEAIDRKK-RSIWRPWWGIGGSDAQSIFSNAGLVVLTDALLYREPQREFMSVMMMDGAPGMAEAAFA-----------APPMGGSSPPPPTVRKFFPHTWIWSDLNSTSGEVEMEIEAPDTITSWVASTFAINEENGLGVAPTTSKLRVFRPFFIQLNLPYAVRRGEKFALLVLVFNYMEKEQDVTVTLKYDKDSGYDLLKKDGTVV--RRDEVGQQNVRIVSVAGGGTSKAVYFPIVPSSIGEIPVHI---------------SAIASQGGDAVEMNLRVDPQGYKVDRNIPFVIDLNNNSSDFSKNLELIWPNDVVDGSQKARLDVIGDMMGPVLNNAHKLVQMPYGCGEQNMLNLVPNILVVKYLRATNRNESQLETKAIKFIEQGIQRELTYKRADNSFSAFGDSDKAGSTWLTAFVVRSFHHAKQYAFVDPNVISRAVAFLNSQQMESGAFAERGEVHHKDMQGGAQDG----GVALTAFVLISILENGMENGKAVTYLEKHLDEVSGNAYTMAVVAYALQLAKSKQAGKAFENLKKHKIVEKSGDVKFASAQKKVEKLKESRAYMFQARPVDIETTSYAVLSYLAQNQTSESLSIIRWLVSQRNELGGFTSTQDTVMALQALSSYA-AVTYSDKHTSQVT-ILNGKHTHSFDINIRNAIVLQSYQLSSLN-DAVSINANGTGVVFAQLS--YSYYR-DSLNDDAPFFCSQEIKEIRAGNRLQLDLCCNYTRPGK-SNMALAEIDALSGY-------RFDAEQVHTLTSIEDLQRVEMEKDDTKMNVYFNPL----GGRPVCLSLYSDVTYQVADQKPANFRLVDYYDPEEQLKMTYAAKQTRSLQEKCGE 1468
BLAST of Alpha-2-macroglobulin vs. Ensembl Celegans
Match: tep-1 (TEP (ThiolEster containing Protein) [Source:UniProtKB/TrEMBL;Acc:Q7JKM0]) HSP 1 Score: 326.25 bits (835), Expect = 2.477e-90 Identity = 273/976 (27.97%), Postives = 455/976 (46.62%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAY-FINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLE-YDSRKVLSTAGFTAITNARIVNVKCMQYLA----RGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTV--PDSMTTWRADSYCMTEK-GLWIAPT-QKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDE-----------------KTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAI--PSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENI-TNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQIST-----PLNRIDFQK--TRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPE-----IYSEKSYSMFNKACNK 4830
IS P NM P +I Y I + EV+ DAL+ +Q+ +K+ P V V K + + + D+++L ++ + Q L + + G R + I +K+ + + +P +G+ D++ + S AG +T+A + ++++ G P + A F+ + + +R++FP +W++S N T+ + + + PD++T+W A ++ + E+ GL +APT KL+V PF+ + +PY+++ E L V VFNY ++ V + D + + K T+ + DE K VYF + P IG++ V I I D+V + V+P G + +I N + P+++V GS ++ L GD+MG +N KLV MPYGCGEQNM+ +VP+I V+ YL + N L KA +I G R+LTYK D SFSAFG +DK GSTWLTAFV++SFH A +Y ++ + + A +L Q G F E+G++ M GG + G LTA+VLIS+ NG++ + K ++ + N T Y L L + + + + D + + K + + ++ DIETT YA+L+ + S S+ + WL +QRN LGG+ STQDTV+AL AL+ +A + S +V+ +LN T++ I + N +V Q++ L + N VS+ NG+G QLS Y+ +R + ND EI ++ N + +C Y R K SNM L I+ SG+ + + Q+ T L R++ +K T+M +Y++ G+ C + ++++ +PA ++VDYY+PE Y+ K + C +
Sbjct: 549 ISFPATANMAPKSRLIVYAIIESSQEVLVDALDFKVEGIFQNQVA---LSIDKQAVEPGQNVKFKVTSD-KNSFVGLLVVDQSVLLLKTGNDITREKVEQDLENYDSN-NVGGGFGGPRPWEAIDRKK-RSIWRPWWGIGGSDAQSIFSNAGLVVLTDALLYREPQREFMSVMMMDGAPGMAEAAFA-----------APPMGGSSPPPPTVRKFFPHTWIWSDLNSTSGEVEMEIEAPDTITSWVASTFAINEENGLGVAPTTSKLRVFRPFFIQLNLPYAVRRGEKFALLVLVFNYMEKEQDVTVTLKYDKDSGYDLLKKDGTVV--RRDEVGQQNVRIVSVAGGGTSKAVYFPIVPSSIGEIPVHI---------------SAIASQGGDAVEMNLRVDPQGYKVDRNIPFVIDLNNNSSDFSKNLELIWPNDVVDGSQKARLDVIGDMMGPVLNNAHKLVQMPYGCGEQNMLNLVPNILVVKYLRATNRNESQLETKAIKFIEQGIQRELTYKRADNSFSAFGDSDKAGSTWLTAFVVRSFHHAKQYAFVDPNVISRAVAFLNSQQMESGAFAERGEVHHKDMQGGAQDG----GVALTAFVLISILENGMENGKAVTYLEKHLDEVSGNAYTMAVVAYALQLAKSKQAGKAFENLKKHKIVEKSGDVKFASAQKKVEKLKESRAYMFQARPVDIETTSYAVLSYLAQNQTSESLSIIRWLVSQRNELGGFTSTQDTVMALQALSSYA-AVTYSDKHTSQVT-ILNGKHTHSFDINIRNAIVLQSYQLSSLN-DAVSINANGTGVVFAQLS--YSYYR-DSLNDDAPFFCSQEIKEIRAGNRLQLDLCCNYTRPGK-SNMALAEIDALSGY-------RFDAEQVHTLTSIEDLQRVEMEKDDTKMNVYFNPL----GGRPVCLSLYSDVTYQVADQKPANFRLVDYYDPEEQLKMTYAAKQTRSLQEKCGE 1468
BLAST of Alpha-2-macroglobulin vs. Ensembl Celegans
Match: tep-1 (TEP (ThiolEster containing Protein) [Source:UniProtKB/TrEMBL;Acc:Q7JKM0]) HSP 1 Score: 325.479 bits (833), Expect = 4.212e-90 Identity = 271/973 (27.85%), Postives = 456/973 (46.87%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAY-FINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLE-YDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSR-NSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTV--PDSMTTWRADSYCMTEK-GLWIAPT-QKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDE-----------------KTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAI--PSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENI-TNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQIST-----PLNRIDFQK--TRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPE-----IYSEKSYSMFNKACNK 4830
IS P NM P +I Y I + EV+ DAL+ +Q+ +K+ P V V K + + + D+++L ++ + Q L + + G R + I +K+ + + +P +G+ D++ + S AG +T+A + ++++ R+ + M+++ + + +R++FP +W++S N T+ + + + PD++T+W A ++ + E+ GL +APT KL+V PF+ + +PY+++ E L V VFNY ++ V + D + + K T+ + DE K VYF + P IG++ V I I D+V + V+P G + +I N + P+++V GS ++ L GD+MG +N KLV MPYGCGEQNM+ +VP+I V+ YL + N L KA +I G R+LTYK D SFSAFG +DK GSTWLTAFV++SFH A +Y ++ + + A +L Q G F E+G++ M GG + G LTA+VLIS+ NG++ + K ++ + N T Y L L + + + + D + + K + + ++ DIETT YA+L+ + S S+ + WL +QRN LGG+ STQDTV+AL AL+ +A + S +V+ +LN T++ I + N +V Q++ L + N VS+ NG+G QLS Y+ +R + ND EI ++ N + +C Y R K SNM L I+ SG+ + + Q+ T L R++ +K T+M +Y++ G+ C + ++++ +PA ++VDYY+PE Y+ K + C +
Sbjct: 549 ISFPATANMAPKSRLIVYAIIESSQEVLVDALDFKVEGIFQNQVA---LSIDKQAVEPGQNVKFKVTSD-KNSFVGLLVVDQSVLLLKTGNDITREKVEQDLENYDSN-NVGGGFGGPRPWEAIDRKK-RSIWRPWWGIGGSDAQSIFSNAGLVVLTDALLYREPQREFMSGTFMRRNKVMMMDGAPGMAEAAFAAPPMGGSSPPPPTVRKFFPHTWIWSDLNSTSGEVEMEIEAPDTITSWVASTFAINEENGLGVAPTTSKLRVFRPFFIQLNLPYAVRRGEKFALLVLVFNYMEKEQDVTVTLKYDKDSGYDLLKKDGTVV--RRDEVGQQNVRIVSVAGGGTSKAVYFPIVPSSIGEIPVHI---------------SAIASQGGDAVEMNLRVDPQGYKVDRNIPFVIDLNNNSSDFSKNLELIWPNDVVDGSQKARLDVIGDMMGPVLNNAHKLVQMPYGCGEQNMLNLVPNILVVKYLRATNRNESQLETKAIKFIEQGIQRELTYKRADNSFSAFGDSDKAGSTWLTAFVVRSFHHAKQYAFVDPNVISRAVAFLNSQQMESGAFAERGEVHHKDMQGGAQDG----GVALTAFVLISILENGMENGKAVTYLEKHLDEVSGNAYTMAVVAYALQLAKSKQAGKAFENLKKHKIVEKSGDVKFASAQKKVEKLKESRAYMFQARPVDIETTSYAVLSYLAQNQTSESLSIIRWLVSQRNELGGFTSTQDTVMALQALSSYA-AVTYSDKHTSQVT-ILNGKHTHSFDINIRNAIVLQSYQLSSLN-DAVSINANGTGVVFAQLS--YSYYR-DSLNDDAPFFCSQEIKEIRAGNRLQLDLCCNYTRPGK-SNMALAEIDALSGY-------RFDAEQVHTLTSIEDLQRVEMEKDDTKMNVYFNPL----GGRPVCLSLYSDVTYQVADQKPANFRLVDYYDPEEQLKMTYAAKQTRSLQEKCGE 1476
BLAST of Alpha-2-macroglobulin vs. Ensembl Celegans
Match: tep-1 (TEP (ThiolEster containing Protein) [Source:UniProtKB/TrEMBL;Acc:Q7JKM0]) HSP 1 Score: 325.479 bits (833), Expect = 4.212e-90 Identity = 271/973 (27.85%), Postives = 456/973 (46.87%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAY-FINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLE-YDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSR-NSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTV--PDSMTTWRADSYCMTEK-GLWIAPT-QKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDE-----------------KTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAI--PSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENI-TNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQIST-----PLNRIDFQK--TRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPE-----IYSEKSYSMFNKACNK 4830
IS P NM P +I Y I + EV+ DAL+ +Q+ +K+ P V V K + + + D+++L ++ + Q L + + G R + I +K+ + + +P +G+ D++ + S AG +T+A + ++++ R+ + M+++ + + +R++FP +W++S N T+ + + + PD++T+W A ++ + E+ GL +APT KL+V PF+ + +PY+++ E L V VFNY ++ V + D + + K T+ + DE K VYF + P IG++ V I I D+V + V+P G + +I N + P+++V GS ++ L GD+MG +N KLV MPYGCGEQNM+ +VP+I V+ YL + N L KA +I G R+LTYK D SFSAFG +DK GSTWLTAFV++SFH A +Y ++ + + A +L Q G F E+G++ M GG + G LTA+VLIS+ NG++ + K ++ + N T Y L L + + + + D + + K + + ++ DIETT YA+L+ + S S+ + WL +QRN LGG+ STQDTV+AL AL+ +A + S +V+ +LN T++ I + N +V Q++ L + N VS+ NG+G QLS Y+ +R + ND EI ++ N + +C Y R K SNM L I+ SG+ + + Q+ T L R++ +K T+M +Y++ G+ C + ++++ +PA ++VDYY+PE Y+ K + C +
Sbjct: 549 ISFPATANMAPKSRLIVYAIIESSQEVLVDALDFKVEGIFQNQVA---LSIDKQAVEPGQNVKFKVTSD-KNSFVGLLVVDQSVLLLKTGNDITREKVEQDLENYDSN-NVGGGFGGPRPWEAIDRKK-RSIWRPWWGIGGSDAQSIFSNAGLVVLTDALLYREPQREFMSGTFMRRNKVMMMDGAPGMAEAAFAAPPMGGSSPPPPTVRKFFPHTWIWSDLNSTSGEVEMEIEAPDTITSWVASTFAINEENGLGVAPTTSKLRVFRPFFIQLNLPYAVRRGEKFALLVLVFNYMEKEQDVTVTLKYDKDSGYDLLKKDGTVV--RRDEVGQQNVRIVSVAGGGTSKAVYFPIVPSSIGEIPVHI---------------SAIASQGGDAVEMNLRVDPQGYKVDRNIPFVIDLNNNSSDFSKNLELIWPNDVVDGSQKARLDVIGDMMGPVLNNAHKLVQMPYGCGEQNMLNLVPNILVVKYLRATNRNESQLETKAIKFIEQGIQRELTYKRADNSFSAFGDSDKAGSTWLTAFVVRSFHHAKQYAFVDPNVISRAVAFLNSQQMESGAFAERGEVHHKDMQGGAQDG----GVALTAFVLISILENGMENGKAVTYLEKHLDEVSGNAYTMAVVAYALQLAKSKQAGKAFENLKKHKIVEKSGDVKFASAQKKVEKLKESRAYMFQARPVDIETTSYAVLSYLAQNQTSESLSIIRWLVSQRNELGGFTSTQDTVMALQALSSYA-AVTYSDKHTSQVT-ILNGKHTHSFDINIRNAIVLQSYQLSSLN-DAVSINANGTGVVFAQLS--YSYYR-DSLNDDAPFFCSQEIKEIRAGNRLQLDLCCNYTRPGK-SNMALAEIDALSGY-------RFDAEQVHTLTSIEDLQRVEMEKDDTKMNVYFNPL----GGRPVCLSLYSDVTYQVADQKPANFRLVDYYDPEEQLKMTYAAKQTRSLQEKCGE 1476
BLAST of Alpha-2-macroglobulin vs. Ensembl Fly
Match: Tep2 (gene:FBgn0041182 transcript:FBtr0079511) HSP 1 Score: 290.812 bits (743), Expect = 2.543e-79 Identity = 238/767 (31.03%), Postives = 376/767 (49.02%), Query Frame = 1
Query: 2641 KKQIREYFPESWVFSYF-NKTTKHLNLT--VPDSMTTWRADSYCMTEKGLWIAPTQ---KLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVD--------ANEI------NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFA-IPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQ------KSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYL-----RRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQ--KTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
+ Q+R+ FPE+W+F+ F N + LT +PD++T+W + + IA T+ K++V PF+ + +PYS+K EV+ +PV +FNY + +V + N D NE+ V +K T+ N K+V F ++PK +G + I + D++ +++ VEP GV + I K++ S+ A IP+E+V S + GD++G T NLD LV MPYGCGEQNMV VP+I VL YL K P++ +KA+ ++ GY R+LTYKHDDGS+SAFGK+D GSTWLTA+V++SFH A Y +I+ + +L Q+ G F E GKLF N + LT++VL++ N + I KA+ E Y +L ++L S+ V A+L + +W ES+ E K S D+E T Y +LAL+ ++ + WL +QRNS GG+ STQDTV+ L AL FA KT S ++++ S T + +N LV QT +L ++ V G+G A +QLS YN+ +FK++ T D+ Q I + VC Y+ +DK+SNM ++ I PSG+ + +Q + R++ + + + +Y+D+ + D C + + ++ +PA V + DYY+ E + + Y + + C+
Sbjct: 660 EPQVRKEFPENWIFNIFENVGEEEFTLTKKIPDTITSWVVTGFSLNPTS-GIALTKNPSKIRVFQPFFVSTNLPYSVKRGEVIAIPVVIFNYLDKTLDADVVMDNSDQEYEFTEATNEVLEKAIDEVRRVKRVTIPA--NSGKSVSFMIRPKNVGFTTLKI---------------TATSALAGDAIHQKLKVEPEGVTLFENRAVFINLKDQPEMSQSLDADIPNEVVPQSEFIEFSVVGDLLGPTLQNLDNLVRMPYGCGEQNMVNFVPNILVLKYLEVTGRKLPSVESKARKFLEIGYQRELTYKHDDGSYSAFGKSDASGSTWLTAYVMRSFHQAGTYTDIDPKVITAGLDFLVSKQKESGEFPEVGKLFD---------NANQNPLALTSFVLLAFFENHELIPKYQSAIKKAVRYVAEEADKTDDQY--SLAIAAVALQLAKHPQSEKVIAKLESVARKENDRMWWSKATESTGEDGRVFHWKPRSNDVEITSYVLLALLEKDPAEKALPIIKWLISQRNSNGGFSSTQDTVIGLQALTKFAYKTGSGSGTMDIEFSSAGESKNTIK--VNPENSLVLQTHDL-PKSTRKVDFTAKGTGSAMVQLSYRYNLAE-KEKKPSFKVT-PTVKDTPNQLLI--VDVCAEYVPLEDADKDKDSNMAVMEIALPSGF--VGDSTSLGKIQAVDRVKRVETKNSDSTVVVYFDSLTPGDV---RCLPLEASKAHAVAKQKPASVSLYDYYDTERKATEYYQVKSSLCD 1385 HSP 2 Score: 78.5666 bits (192), Expect = 6.056e-14 Identity = 58/199 (29.15%), Postives = 94/199 (47.24%), Query Frame = 1
Query: 361 TKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQ-EEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYG 951
+ ++G + ++ DK +F +TDK YKP D V+ R + LD+N ++P+K + +III+ N R+ Q +V + + + + E QL++ P+ G WK DG E FE+ KYVLP+F V V P ++ D+ I + +KY+ GKP+ K + M SYG
Sbjct: 93 AEGVSGVVFKNSTKLNYADKKPSVFVQTDKATYKPADLVQFRIL--------------------FLDEN----TRPAKIEKP---ISVIIIDGAQN-RIKQLSDVKLTKGVFSGELQLSEQPVLGTWKISVSVDGDNRETKSFEVDKYVLPKFEVIVDTPKAVVIADKVIKATIRAKYTYGKPVKGKAT--VSMERSYG 261
BLAST of Alpha-2-macroglobulin vs. Ensembl Fly
Match: Tep2 (gene:FBgn0041182 transcript:FBtr0079510) HSP 1 Score: 288.115 bits (736), Expect = 1.994e-78 Identity = 235/761 (30.88%), Postives = 369/761 (48.49%), Query Frame = 1
Query: 2650 IREYFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQ---KLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVD--------ANEI------NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFA-IPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQ------KSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYL-----RRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQ--KTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
IR+ FPE+W F + L +PD++T+W + + IA T+ K++V PF+ + +PYS+K EV+ +PV +FNY + +V + N D NE+ V +K T+ N K+V F ++PK +G + I + D++ +++ VEP GV + I K++ S+ A IP+E+V S + GD++G T NLD LV MPYGCGEQNMV VP+I VL YL K P++ +KA+ ++ GY R+LTYKHDDGS+SAFGK+D GSTWLTA+V++SFH A Y +I+ + +L Q+ G F E GKLF N + LT++VL++ N + I KA+ E Y +L ++L S+ V A+L + +W ES+ E K S D+E T Y +LAL+ ++ + WL +QRNS GG+ STQDTV+ L AL FA KT S ++++ S T + +N LV QT +L ++ V G+G A +QLS YN+ +FK++ T D+ Q I + VC Y+ +DK+SNM ++ I PSG+ + +Q + R++ + + + +Y+D+ + D C + + ++ +PA V + DYY+ E + + Y + + C+
Sbjct: 687 IRKEFPETWFFENVGEEEFTLTKKIPDTITSWVVTGFSLNPTS-GIALTKNPSKIRVFQPFFVSTNLPYSVKRGEVIAIPVVIFNYLDKTLDADVVMDNSDQEYEFTEATNEVLEKAIDEVRRVKRVTIPA--NSGKSVSFMIRPKNVGFTTLKI---------------TATSALAGDAIHQKLKVEPEGVTLFENRAVFINLKDQPEMSQSLDADIPNEVVPQSEFIEFSVVGDLLGPTLQNLDNLVRMPYGCGEQNMVNFVPNILVLKYLEVTGRKLPSVESKARKFLEIGYQRELTYKHDDGSYSAFGKSDASGSTWLTAYVMRSFHQAGTYTDIDPKVITAGLDFLVSKQKESGEFPEVGKLFD---------NANQNPLALTSFVLLAFFENHELIPKYQSAIKKAVRYVAEEADKTDDQY--SLAIAAVALQLAKHPQSEKVIAKLESVARKENDRMWWSKATESTGEDGRVFHWKPRSNDVEITSYVLLALLEKDPAEKALPIIKWLISQRNSNGGFSSTQDTVIGLQALTKFAYKTGSGSGTMDIEFSSAGESKNTIK--VNPENSLVLQTHDL-PKSTRKVDFTAKGTGSAMVQLSYRYNLAE-KEKKPSFKVT-PTVKDTPNQLLI--VDVCAEYVPLEDADKDKDSNMAVMEIALPSGF--VGDSTSLGKIQAVDRVKRVETKNSDSTVVVYFDSLTPGDV---RCLPLEASKAHAVAKQKPASVSLYDYYDTERKATEYYQVKSSLCD 1406 HSP 2 Score: 78.5666 bits (192), Expect = 5.498e-14 Identity = 58/199 (29.15%), Postives = 94/199 (47.24%), Query Frame = 1
Query: 361 TKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQ-EEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYG 951
+ ++G + ++ DK +F +TDK YKP D V+ R + LD+N ++P+K + +III+ N R+ Q +V + + + + E QL++ P+ G WK DG E FE+ KYVLP+F V V P ++ D+ I + +KY+ GKP+ K + M SYG
Sbjct: 93 AEGVSGVVFKNSTKLNYADKKPSVFVQTDKATYKPADLVQFRIL--------------------FLDEN----TRPAKIEKP---ISVIIIDGAQN-RIKQLSDVKLTKGVFSGELQLSEQPVLGTWKISVSVDGDNRETKSFEVDKYVLPKFEVIVDTPKAVVIADKVIKATIRAKYTYGKPVKGKAT--VSMERSYG 261
BLAST of Alpha-2-macroglobulin vs. Ensembl Fly
Match: Tep2 (gene:FBgn0041182 transcript:FBtr0335488) HSP 1 Score: 287.345 bits (734), Expect = 3.450e-78 Identity = 238/765 (31.11%), Postives = 374/765 (48.89%), Query Frame = 1
Query: 2647 QIREYFPESWVF-SYFNKTTKHLNLT--VPDSMTTWRADSYCMTEKGLWIAPTQ---KLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVD--------ANEI------NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFA-IPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQ------KSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYL-----RRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQ--KTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
QIR+ FPE+W+F + N + LT +PD++T+W + + IA T+ K++V PF+ + +PYS+K EV+ +PV +FNY + +V + N D NE+ V +K T+ N K+V F ++PK +G + I + D++ +++ VEP GV + I K++ S+ A IP+E+V S + GD++G T NLD LV MPYGCGEQNMV VP+I VL YL K P++ +KA+ ++ GY R+LTYKHDDGS+SAFGK+D GSTWLTA+V++SFH A Y +I+ + +L Q+ G F E GKLF N + LT++VL++ N + I KA+ E Y +L ++L S+ V A+L + +W ES+ E K S D+E T Y +LAL+ ++ + WL +QRNS GG+ STQDTV+ L AL FA KT S ++++ S T + +N LV QT +L ++ V G+G A +QLS YN+ +FK++ T D+ Q I + VC Y+ +DK+SNM ++ I PSG+ + +Q + R++ + + + +Y+D+ + D C + + ++ +PA V + DYY+ E + + Y + + C+
Sbjct: 672 QIRKEFPENWIFYNAENVGEEEFTLTKKIPDTITSWVVTGFSLNPTS-GIALTKNPSKIRVFQPFFVSTNLPYSVKRGEVIAIPVVIFNYLDKTLDADVVMDNSDQEYEFTEATNEVLEKAIDEVRRVKRVTIPA--NSGKSVSFMIRPKNVGFTTLKI---------------TATSALAGDAIHQKLKVEPEGVTLFENRAVFINLKDQPEMSQSLDADIPNEVVPQSEFIEFSVVGDLLGPTLQNLDNLVRMPYGCGEQNMVNFVPNILVLKYLEVTGRKLPSVESKARKFLEIGYQRELTYKHDDGSYSAFGKSDASGSTWLTAYVMRSFHQAGTYTDIDPKVITAGLDFLVSKQKESGEFPEVGKLFD---------NANQNPLALTSFVLLAFFENHELIPKYQSAIKKAVRYVAEEADKTDDQY--SLAIAAVALQLAKHPQSEKVIAKLESVARKENDRMWWSKATESTGEDGRVFHWKPRSNDVEITSYVLLALLEKDPAEKALPIIKWLISQRNSNGGFSSTQDTVIGLQALTKFAYKTGSGSGTMDIEFSSAGESKNTIK--VNPENSLVLQTHDL-PKSTRKVDFTAKGTGSAMVQLSYRYNLAE-KEKKPSFKVT-PTVKDTPNQLLI--VDVCAEYVPLEDADKDKDSNMAVMEIALPSGF--VGDSTSLGKIQAVDRVKRVETKNSDSTVVVYFDSLTPGDV---RCLPLEASKAHAVAKQKPASVSLYDYYDTERKATEYYQVKSSLCD 1395 HSP 2 Score: 78.9518 bits (193), Expect = 5.127e-14 Identity = 58/199 (29.15%), Postives = 94/199 (47.24%), Query Frame = 1
Query: 361 TKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQ-EEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYG 951
+ ++G + ++ DK +F +TDK YKP D V+ R + LD+N ++P+K + +III+ N R+ Q +V + + + + E QL++ P+ G WK DG E FE+ KYVLP+F V V P ++ D+ I + +KY+ GKP+ K + M SYG
Sbjct: 93 AEGVSGVVFKNSTKLNYADKKPSVFVQTDKATYKPADLVQFRIL--------------------FLDEN----TRPAKIEKP---ISVIIIDGAQN-RIKQLSDVKLTKGVFSGELQLSEQPVLGTWKISVSVDGDNRETKSFEVDKYVLPKFEVIVDTPKAVVIADKVIKATIRAKYTYGKPVKGKAT--VSMERSYG 261
BLAST of Alpha-2-macroglobulin vs. Ensembl Fly
Match: Tep2 (gene:FBgn0041182 transcript:FBtr0079513) HSP 1 Score: 280.026 bits (715), Expect = 7.687e-76 Identity = 232/766 (30.29%), Postives = 370/766 (48.30%), Query Frame = 1
Query: 2647 QIREYFPESWVFSYFNKTTKHLNLT----VPDSMTTWRADSYCMTEKGLWIAPTQ---KLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVD--------ANEI------NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFA-IPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQ------KSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYL-----RRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQ--KTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
+IR+ F + W++ ++ T +PD++T+W + + IA T+ K++V PF+ + +PYS+K EV+ +PV +FNY + +V + N D NE+ V +K T+ N K+V F ++PK +G + I + D++ +++ VEP GV + I K++ S+ A IP+E+V S + GD++G T NLD LV MPYGCGEQNMV VP+I VL YL K P++ +KA+ ++ GY R+LTYKHDDGS+SAFGK+D GSTWLTA+V++SFH A Y +I+ + +L Q+ G F E GKLF N + LT++VL++ N + I KA+ E Y +L ++L S+ V A+L + +W ES+ E K S D+E T Y +LAL+ ++ + WL +QRNS GG+ STQDTV+ L AL FA KT S ++++ S T + +N LV QT +L ++ V G+G A +QLS YN+ +FK++ T D+ Q I + VC Y+ +DK+SNM ++ I PSG+ + +Q + R++ + + + +Y+D+ + D C + + ++ +PA V + DYY+ E + + Y + + C+
Sbjct: 670 EIRKNFADVWIWQSIGRSVGEEEFTLTKKIPDTITSWVVTGFSLNPTS-GIALTKNPSKIRVFQPFFVSTNLPYSVKRGEVIAIPVVIFNYLDKTLDADVVMDNSDQEYEFTEATNEVLEKAIDEVRRVKRVTIPA--NSGKSVSFMIRPKNVGFTTLKI---------------TATSALAGDAIHQKLKVEPEGVTLFENRAVFINLKDQPEMSQSLDADIPNEVVPQSEFIEFSVVGDLLGPTLQNLDNLVRMPYGCGEQNMVNFVPNILVLKYLEVTGRKLPSVESKARKFLEIGYQRELTYKHDDGSYSAFGKSDASGSTWLTAYVMRSFHQAGTYTDIDPKVITAGLDFLVSKQKESGEFPEVGKLFD---------NANQNPLALTSFVLLAFFENHELIPKYQSAIKKAVRYVAEEADKTDDQY--SLAIAAVALQLAKHPQSEKVIAKLESVARKENDRMWWSKATESTGEDGRVFHWKPRSNDVEITSYVLLALLEKDPAEKALPIIKWLISQRNSNGGFSSTQDTVIGLQALTKFAYKTGSGSGTMDIEFSSAGESKNTIK--VNPENSLVLQTHDL-PKSTRKVDFTAKGTGSAMVQLSYRYNLAE-KEKKPSFKVT-PTVKDTPNQLLI--VDVCAEYVPLEDADKDKDSNMAVMEIALPSGF--VGDSTSLGKIQAVDRVKRVETKNSDSTVVVYFDSLTPGDV---RCLPLEASKAHAVAKQKPASVSLYDYYDTERKATEYYQVKSSLCD 1394 HSP 2 Score: 78.1814 bits (191), Expect = 7.882e-14 Identity = 58/199 (29.15%), Postives = 94/199 (47.24%), Query Frame = 1
Query: 361 TKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQ-EEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYG 951
+ ++G + ++ DK +F +TDK YKP D V+ R + LD+N ++P+K + +III+ N R+ Q +V + + + + E QL++ P+ G WK DG E FE+ KYVLP+F V V P ++ D+ I + +KY+ GKP+ K + M SYG
Sbjct: 93 AEGVSGVVFKNSTKLNYADKKPSVFVQTDKATYKPADLVQFRIL--------------------FLDEN----TRPAKIEKP---ISVIIIDGAQN-RIKQLSDVKLTKGVFSGELQLSEQPVLGTWKISVSVDGDNRETKSFEVDKYVLPKFEVIVDTPKAVVIADKVIKATIRAKYTYGKPVKGKAT--VSMERSYG 261
BLAST of Alpha-2-macroglobulin vs. Ensembl Fly
Match: Tep2 (gene:FBgn0041182 transcript:FBtr0335487) HSP 1 Score: 275.404 bits (703), Expect = 1.706e-74 Identity = 233/766 (30.42%), Postives = 371/766 (48.43%), Query Frame = 1
Query: 2650 IREYFPESWVFSYFNKTT---KHLNLT--VPDSMTTWRADSYCMTEKGLWIAPTQ---KLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVD--------ANEI------NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFA-IPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQ------KSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYL-----RRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQ--KTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
+R F E W+++ + + + LT +PD++T+W + + IA T+ K++V PF+ + +PYS+K EV+ +PV +FNY + +V + N D NE+ V +K T+ N K+V F ++PK +G + I + D++ +++ VEP GV + I K++ S+ A IP+E+V S + GD++G T NLD LV MPYGCGEQNMV VP+I VL YL K P++ +KA+ ++ GY R+LTYKHDDGS+SAFGK+D GSTWLTA+V++SFH A Y +I+ + +L Q+ G F E GKLF N + LT++VL++ N + I KA+ E Y +L ++L S+ V A+L + +W ES+ E K S D+E T Y +LAL+ ++ + WL +QRNS GG+ STQDTV+ L AL FA KT S ++++ S T + +N LV QT +L ++ V G+G A +QLS YN+ +FK++ T D+ Q I + VC Y+ +DK+SNM ++ I PSG+ + +Q + R++ + + + +Y+D+ + D C + + ++ +PA V + DYY+ E + + Y + + C+
Sbjct: 662 VRTNFAEVWMWTTSDNGSVGEEEFTLTKKIPDTITSWVVTGFSLNPTS-GIALTKNPSKIRVFQPFFVSTNLPYSVKRGEVIAIPVVIFNYLDKTLDADVVMDNSDQEYEFTEATNEVLEKAIDEVRRVKRVTIPA--NSGKSVSFMIRPKNVGFTTLKI---------------TATSALAGDAIHQKLKVEPEGVTLFENRAVFINLKDQPEMSQSLDADIPNEVVPQSEFIEFSVVGDLLGPTLQNLDNLVRMPYGCGEQNMVNFVPNILVLKYLEVTGRKLPSVESKARKFLEIGYQRELTYKHDDGSYSAFGKSDASGSTWLTAYVMRSFHQAGTYTDIDPKVITAGLDFLVSKQKESGEFPEVGKLFD---------NANQNPLALTSFVLLAFFENHELIPKYQSAIKKAVRYVAEEADKTDDQY--SLAIAAVALQLAKHPQSEKVIAKLESVARKENDRMWWSKATESTGEDGRVFHWKPRSNDVEITSYVLLALLEKDPAEKALPIIKWLISQRNSNGGFSSTQDTVIGLQALTKFAYKTGSGSGTMDIEFSSAGESKNTIK--VNPENSLVLQTHDL-PKSTRKVDFTAKGTGSAMVQLSYRYNLAE-KEKKPSFKVT-PTVKDTPNQLLI--VDVCAEYVPLEDADKDKDSNMAVMEIALPSGF--VGDSTSLGKIQAVDRVKRVETKNSDSTVVVYFDSLTPGDV---RCLPLEASKAHAVAKQKPASVSLYDYYDTERKATEYYQVKSSLCD 1386 HSP 2 Score: 78.5666 bits (192), Expect = 5.474e-14 Identity = 58/198 (29.29%), Postives = 93/198 (46.97%), Query Frame = 1
Query: 364 KNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQ-EEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYG 951
+ ++G + ++ DK +F +TDK YKP D V+ R + LD+N ++P+K + +III+ N R+ Q +V + + + + E QL++ P+ G WK DG E FE+ KYVLP+F V V P ++ D+ I + +KY+ GKP+ K M SYG
Sbjct: 94 EGVSGVVFKNSTKLNYADKKPSVFVQTDKATYKPADLVQFRIL--------------------FLDEN----TRPAKIEKP---ISVIIIDGAQN-RIKQLSDVKLTKGVFSGELQLSEQPVLGTWKISVSVDGDNRETKSFEVDKYVLPKFEVIVDTPKAVVIADKVIKATIRAKYTYGKPVKGK--ATVSMERSYG 261
BLAST of Alpha-2-macroglobulin vs. Ensembl Zebrafish
Match: si:ch211-212c13.8 (si:ch211-212c13.8 [Source:ZFIN;Acc:ZDB-GENE-131118-28]) HSP 1 Score: 343.199 bits (879), Expect = 2.486e-97 Identity = 261/947 (27.56%), Postives = 456/947 (48.15%), Query Frame = 1
Query: 2056 NMGPDVHVIAYFINTDNEVVA--DALEIASGLNNCSQLGGGKFKFNKKTAMP--KSKVVLTVDPGVKENGCAIRIADEA--LLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSR----NSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDAN-EINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQ-----DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDS-VKQCNIRN--IKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQIST-------PLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
++ P V ++AY + ++A +L+I N L +F+ A+P K+ + L+ PG + C + D++ +L+ G+ +AD + + + + + + + C+P R +R + L + L + G +N + +C+ + RG+ +Y F + + R +S+ + IR FPE+W++ + + + +TVPD++TTW +++C++ GL +AP +L V PF+ + +PYS+ E+ +L VFNY C + V ++ + ++ C C N +T + L P ++G +NVT+ + ++ S + + + D V R +LV+ G+ T S L+C K + L +P +++ GS +S ++ GD++G+ NL L+ MPYGCGEQNM + P+IY+L YL + A+ KA S++ GY RQL YKH DG++S FG D G+TWLTAFVL+SF A KY I+ ++ A +L + SDGCF ++G+LF++ M GG+ D +TAY+ SL ++ T T + ++ + N ++ Q +F++L D ++S G +W +S+ S S D+E + Y +LA++ DL + + V WL Q+N+ GG+ STQDTVVAL AL+++A + S + V + +++ + DN+L+ Q L Y S++ GS C S+Q++ YNI A LS+ E++ K+ +N + V Y +NM++V I SG+ K+ + P+ RID + + +Y + D + + + ++ NL+PAV+K+ DYY SE YS
Sbjct: 233 DVAPVVRILAYCVLPSENIIAASKSLDIEKCFGNKVSL-----QFSPAKAVPGEKNTLQLSAQPG---SLCGLSAVDQSVLILESGKRLDADKIFSLLPFQYVYYPYSAED------EQPCLP-VRPRRSV-----LTDNVYNSLKSVGLKMASNLAVRVPECLSF--RGL---TYHRFRDIVMYHTSGRVGLGSSIESSTFDNSAVTIRTIFPETWIWDLAEVGDSGSTEVPVTVPDTITTWETEAFCLSSTGLGLAPPAQLTVFQPFFLELSLPYSIIRGEIFELKATVFNYLSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRQTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDIVTRSLLVQAEGIEKTETNSWLLCPKGDSLSENVDLTVPKDVIEGSAKSSVSVIGDILGRALQNLHGLLRMPYGCGEQNMAVLSPNIYILQYLENTKQLTSAIREKASSFLKSGYQRQLNYKHFDGAYSTFGYGD--GNTWLTAFVLRSFGKAQKYTFIDPQIIQSAKDWLISRRDSDGCFIQQGRLFNNRMKGGV-----TDNVTMTAYITASLLELE-TPVTDPVVTKGLSCLRSVIEDVKNTYTTALLAYTFSLARDTNTRQQLFSKLEDLAISDGPLVHW---SQSASADDSASLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSS--TVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKY-SIEVKGSACVSVQMAQFYNIPTPTEAK---TLSIDAEVEGDCKKTFGQNLMLNVTVTYDGPQNRTNMVIVDIKLLSGFTADTSMLKTQRQSFDSLDFFGHAPVERIDSKDDHVLVYLEEVLKEDPVN---LQIGLNQIIQVKNLKPAVIKVYDYYQTSDQSEMEYS 1134
BLAST of Alpha-2-macroglobulin vs. Ensembl Zebrafish
Match: si:dkey-105h12.2 (si:dkey-105h12.2 [Source:ZFIN;Acc:ZDB-GENE-131118-9]) HSP 1 Score: 346.665 bits (888), Expect = 7.564e-97 Identity = 235/796 (29.52%), Postives = 394/796 (49.50%), Query Frame = 1
Query: 2470 LSTAGFTAITNARIVNVKCMQYLARGMP-DRSYA---TFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDAN-EINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQ-----DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDS-VKQCNIRNIKVCF--AYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
L G +N + +C+ Y RG+ R A + R++M SR ++ IR FPE+W++ + + + +TVPD++TTW +++C++ GL +AP +L V PF+ + +PYS+ E+ +L VFNY C + V ++ + ++ C C N +T + L P ++G +N+T+ + ++ S + + + D V R +LV+ G T S L+C K + L +P +++ GS +S ++ GD++G+ NL L+ MPYGCGEQNM + P+IY+L YL + A+ KA S++ GY RQL YKH DG++S FG D G+TWLTAFVLKSF A KY I+ ++ A +L + SDGCF ++G+LF++ M GG+ N +TAY+ SL ++ T T + ++ + N ++ Q +F +L D ++S G +W +S+ S S D+E + Y +LA++ DL + + V WL Q+N+ GG+ STQDTVVAL AL+++A + S + V + +++ + DN+L+ Q L Y S++ GS C S+Q++ YNI + LS+ E++ K+ +++ + F Y +NM++V I SG+ K+ ++P+ RID + + +Y D + + + ++ NL+PAV+K+ DYY SE YS
Sbjct: 663 LKRVGLKMASNLFVRVPECLSY--RGLTYHRKIARTGVVNTARSRMGFSRPESSLVNRDSPTVTIRTVFPETWIWDLAEVGDSGSAEVPVTVPDTITTWETEAFCLSSTGLGLAPPAQLTVFQPFFLELSLPYSIIRGEIFELKATVFNYLSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRQTFKWILTPSVLGVMNITVSAEAEASQTVCDNEIVSVPERGRIDIVTRSLLVQAEGTEKTETNSWLLCPKGDSLSEEMNLTLPKDVIEGSAKSSVSVIGDILGRALQNLHGLLRMPYGCGEQNMAVLSPNIYILQYLENTKQLTSAIREKASSFLKSGYQRQLNYKHFDGAYSTFGYGD--GNTWLTAFVLKSFGKAQKYTFIDPQIIQSAKDWLISRRNSDGCFIQQGRLFNNRMKGGVNNN-----VTMTAYITASLLELE-TPVTDPVITKGLSCLRSVIEDVKNTYTTALLAYTFSLARDTNTRQQLFNKLEDLAISDGPLVHW---SQSASADDSASLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSS--TVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKY-SIEVKGSACVSVQMAQFYNI---LTPTEAKTLSIDAEVEGDCKKTFGKSLLLNFTVTYDGPQAKTNMVIVDIKLLSGFTADTSMLKTQQQSYASPVERIDSKDDHVLVYLKEVPKADPVN---LQIGLNQIIQVKNLKPAVIKVYDYYQTSDQSEMEYS 1436 HSP 2 Score: 87.8113 bits (216), Expect = 1.278e-16 Identity = 94/349 (26.93%), Postives = 148/349 (42.41%), Query Frame = 1
Query: 346 MKCELTKNITGNFINETREIGLIDKNF--IIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINE-AMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC---MLYSYGKK---KCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKS-QINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWG--FIRNYTSNKKGVIEF 1350
MK EL G T E ++ K++ + F +TDKPIY PG V R + LDKN + D Y +++ ++ DN R+ QW NVS + Y+LN + G +K KA F G+ I F++KKYVLP+F V V P+++ +E + +VC+KY+ G+P+ K C M + + K C +++ K C I L S+ + S + VTE GT +++K+ P + Y + P Y+ G G+I L P + + G NW + N T++ G+ F
Sbjct: 101 MKVELQ----GESFKRTEERKVMFKSYHPLTFIQTDKPIYIPGQTVNFRI--------------------VTLDKNFAPL--------DQQYSSVVLEDSQDN-RIGQWTNVSSTRWILQRSYELNPECREGTYKLKA-FIGERMISHYFKVKKYVLPKFEVAVKKPNKVSVDEEELLIEVCAKYTYGQPVPGKSWVKVCRNSMRHFHTKDFNPLCSEETTEITKAGCAIHNF--GISVFLNSTLKERLMNSLHVEAVVTEEGTEITMTKSESVP-LTYEIGKVTLIDLPKTYEHGSVIEGKIKLFDFKDAPIPNKEVYL-----LEGENWSSKLLLNLTTDSDGLASF 407
BLAST of Alpha-2-macroglobulin vs. Ensembl Zebrafish
Match: a2ml (alpha-2-macroglobulin-like [Source:ZFIN;Acc:ZDB-GENE-090212-1]) HSP 1 Score: 345.125 bits (884), Expect = 1.969e-96 Identity = 265/944 (28.07%), Postives = 453/944 (47.99%), Query Frame = 1
Query: 2056 NMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGK--FKFNKKTAMP--KSKVVLTVDPGVKENGCAIRIADEA--LLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMP-DRSYATFSNER---TQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDAN-EINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQ-----DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEID--SVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKA 4821
++ P V ++AY + V+A A+ + + G K +F+ A+P K+ + L+ PG + C + D++ +L+ G+ +AD K+ L ++ + + R +R + L ++ + L + G TN + C+ Y RG+ ++ + + R + V IR FPE+W++ + + + +TVPD++TTW +++C++ GL +AP +L V PF+ + +PYS+ E+ +L VFNY C + V ++ + ++ C C N KT + L P ++G +NVT+ + ++ S + + + D V R +LV+ G+ T S L+C K + L +P +++ GS +S ++ GD++G+ NL L+ MPYGCGEQNM + P+IY+L YL + A+ KA S++ GY RQL YKH DG++S FG D G+TWLTAFVL+SF A KY I+ ++ A +L + SDGCF ++G+LF++ M GG+ D +TAY+ SL ++ T T + ++ N ++ Q +F +L D ++S G +W S+ S S D+E + Y +LA++ DL + + V WL Q+N+ GG+ STQDTVVAL AL+++A + S + V + +++ + DN+L+ Q L Y S++ GS C S+Q++ YNI A T + K E D ++ Q I + V Y ++ +NM +V I SG+ + S + + R+D + + +Y S N A ++ ++++ ++ NL+PAVVK+ DYY SE YS +A
Sbjct: 535 DVAPVVQILAYCVLPSENVIA-----ANKRLDTEKCFGNKVSLQFSPAKAVPGEKNTLQLSAQPG---SLCGLSAVDQSVLILESGKRLDAD------KIFNLLPVQSVSYYPYSVEDEQACLYVRPRRSV-----LTDNTYETLKSVGLKMATNLAVRVPDCLSY--RGLTYHKNLVMYPDHRPVPVMFALGMAGVDGPVGNSPSVTIRTVFPETWIWELAEVGDSGSAEVPVTVPDTITTWETEAFCLSSTGLGLAPPAQLTVFQPFFLELSLPYSIIRGEIFELKATVFNYLSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDIVTRSLLVQAEGIEKTETNSWLLCPKGDSLSEKVDLTLPKDVIEGSAKSSVSVIGDILGRALQNLHGLLRMPYGCGEQNMAVLSPNIYILQYLENTKQLTSAIREKASSFLKSGYQRQLNYKHFDGAYSTFGYGD--GNTWLTAFVLRSFGKAQKYTFIDPQIIQSAKDWLISRRDSDGCFIQQGRLFNNRMKGGVN-----DNVTMTAYITASLLELE-TPVTDPVITKGLSCLRSVIEEVKNTYTTALLAYTFSLARDTNTRQQLFNKLEDLAISDGPLVHW---SRSASADDSASLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSS--TVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKY-SIEVKGSACVSVQIAQFYNIPTPTEAK-TLSIDAKIEGDCEALGQNFIFDFTV--KYDGPEEKTNMAIVDIKLLSGFTADTSALGTSSGTYVSLVERVDSKDDHVIVYLKEISKNVAMN---YQIQMKQVLQVKNLKPAVVKVYDYYQTSDQSEIEYSFHCEA 1437 HSP 2 Score: 83.5741 bits (205), Expect = 2.141e-15 Identity = 87/330 (26.36%), Postives = 138/330 (41.82%), Query Frame = 1
Query: 385 INETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINE-AMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC---MLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYS-KAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWG--FIRNYTSNKKGVIEF 1350
+ E R++ + + F +TDKPIY PG V R + + TN +D+ F K DN R+ QW NVS + Y+LN + G +K KA + F++KKYVLP+F V V P + +E + +VC+KY+ G+P+ K C + Y C+ ++ K C + IL S+ + + K + VTE GT +++K+ E + Y I P Y+PG G+I L + K A + N + + G NW + N T++ G+ F
Sbjct: 112 MTEERKVMFKSYDPLTFIQTDKPIYMPGQIVNFRVVTM-DTNFAP------------VDQQCVFGIKQDSQDN----------------RIGQWTNVSSTRWILQRSYELNPECREGAYKLKAFIGERMSSHYFQVKKYVLPKFEVTVKKPKTVSVVEEELLIEVCAKYTYGQPVPGKSWVKVCRNPLPYIIFFPLCLEETLEITKTGCAIHNF--GLSMILNSTLKDNLKDSLHVEAVVTEEGTEITMTKS-ESVHLTYEIGKITLIDLPKTYEPGSVIEGKIKL--SDFKDAPIPNKEVYLLE---GENWSSKLLLNLTTDSDGLASF 404
BLAST of Alpha-2-macroglobulin vs. Ensembl Zebrafish
Match: a2ml (alpha-2-macroglobulin-like [Source:ZFIN;Acc:ZDB-GENE-090212-1]) HSP 1 Score: 344.739 bits (883), Expect = 2.709e-96 Identity = 265/944 (28.07%), Postives = 453/944 (47.99%), Query Frame = 1
Query: 2056 NMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGK--FKFNKKTAMP--KSKVVLTVDPGVKENGCAIRIADEA--LLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMP-DRSYATFSNER---TQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDAN-EINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQ-----DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEID--SVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKA 4821
++ P V ++AY + V+A A+ + + G K +F+ A+P K+ + L+ PG + C + D++ +L+ G+ +AD K+ L ++ + + R +R + L ++ + L + G TN + C+ Y RG+ ++ + + R + V IR FPE+W++ + + + +TVPD++TTW +++C++ GL +AP +L V PF+ + +PYS+ E+ +L VFNY C + V ++ + ++ C C N KT + L P ++G +NVT+ + ++ S + + + D V R +LV+ G+ T S L+C K + L +P +++ GS +S ++ GD++G+ NL L+ MPYGCGEQNM + P+IY+L YL + A+ KA S++ GY RQL YKH DG++S FG D G+TWLTAFVL+SF A KY I+ ++ A +L + SDGCF ++G+LF++ M GG+ D +TAY+ SL ++ T T + ++ N ++ Q +F +L D ++S G +W S+ S S D+E + Y +LA++ DL + + V WL Q+N+ GG+ STQDTVVAL AL+++A + S + V + +++ + DN+L+ Q L Y S++ GS C S+Q++ YNI A T + K E D ++ Q I + V Y ++ +NM +V I SG+ + S + + R+D + + +Y S N A ++ ++++ ++ NL+PAVVK+ DYY SE YS +A
Sbjct: 541 DVAPVVQILAYCVLPSENVIA-----ANKRLDTEKCFGNKVSLQFSPAKAVPGEKNTLQLSAQPG---SLCGLSAVDQSVLILESGKRLDAD------KIFNLLPVQSVSYYPYSVEDEQACLYVRPRRSV-----LTDNTYETLKSVGLKMATNLAVRVPDCLSY--RGLTYHKNLVMYPDHRPVPVMFALGMAGVDGPVGNSPSVTIRTVFPETWIWELAEVGDSGSAEVPVTVPDTITTWETEAFCLSSTGLGLAPPAQLTVFQPFFLELSLPYSIIRGEIFELKATVFNYLSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDIVTRSLLVQAEGIEKTETNSWLLCPKGDSLSEKVDLTLPKDVIEGSAKSSVSVIGDILGRALQNLHGLLRMPYGCGEQNMAVLSPNIYILQYLENTKQLTSAIREKASSFLKSGYQRQLNYKHFDGAYSTFGYGD--GNTWLTAFVLRSFGKAQKYTFIDPQIIQSAKDWLISRRDSDGCFIQQGRLFNNRMKGGVN-----DNVTMTAYITASLLELE-TPVTDPVITKGLSCLRSVIEEVKNTYTTALLAYTFSLARDTNTRQQLFNKLEDLAISDGPLVHW---SRSASADDSASLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSS--TVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKY-SIEVKGSACVSVQIAQFYNIPTPTEAK-TLSIDAKIEGDCEALGQNFIFDFTV--KYDGPEEKTNMAIVDIKLLSGFTADTSALGTSSGTYVSLVERVDSKDDHVIVYLKEISKNVAMN---YQIQMKQVLQVKNLKPAVVKVYDYYQTSDQSEIEYSFHCEA 1443 HSP 2 Score: 87.0409 bits (214), Expect = 2.054e-16 Identity = 84/330 (25.45%), Postives = 140/330 (42.42%), Query Frame = 1
Query: 385 INETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINE-AMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC---MLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYS-KAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWG--FIRNYTSNKKGVIEF 1350
+ E R++ + + F +TDKPIY PG V R + +D N + + + +I++E + R+ QW NVS + Y+LN + G +K KA + F++KKYVLP+F V V P + +E + +VC+KY+ G+P+ K C + Y C+ ++ K C + IL S+ + + K + VTE GT +++K+ E + Y I P Y+PG G+I L + K A + N + + G NW + N T++ G+ F
Sbjct: 118 MTEERKVMFKSYDPLTFIQTDKPIYMPGQIVNFRV--------------------VTMDTNFAPVDQ---------QYSIIVLEDSQDNRIGQWTNVSSTRWILQRSYELNPECREGAYKLKAFIGERMSSHYFQVKKYVLPKFEVTVKKPKTVSVVEEELLIEVCAKYTYGQPVPGKSWVKVCRNPLPYIIFFPLCLEETLEITKTGCAIHNF--GLSMILNSTLKDNLKDSLHVEAVVTEEGTEITMTKS-ESVHLTYEIGKITLIDLPKTYEPGSVIEGKIKL--SDFKDAPIPNKEVYLLE---GENWSSKLLLNLTTDSDGLASF 410
BLAST of Alpha-2-macroglobulin vs. Ensembl Zebrafish
Match: sb:cb37 (sb:cb37 [Source:ZFIN;Acc:ZDB-GENE-030131-9800]) HSP 1 Score: 341.658 bits (875), Expect = 3.330e-95 Identity = 262/947 (27.67%), Postives = 447/947 (47.20%), Query Frame = 1
Query: 2056 NMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGK--FKFNKKTAMP--KSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKC------IPQKRVKRMIQPIYGLEYD-SRKVLSTAGFTAITNARIVNVKCMQY--LARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDAN-EINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQ-----DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEID--SVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKA 4821
++ P V ++AY + V+A A+ + + G K +F+ A+P K+ + L+ PG + C + D+++L +++ ++ D LL H + + ++P L D + + L + G TN + +C+ Y LA Y + ++ V + IR FPE+W++ + + + + VPD++TTW +++C++ GL +AP +L V PF+ + +PYS+ E+ +L VFNY C + V ++ + ++ C C N KT + L P ++G +NVT+ + ++ S + + + D V R +LV+ G+ T S L+C K + L +P +++ GS +S ++ GD++G+ NL L+ MPYGCGEQNM + P+IY+L YL + A+ KA S++ GY RQL YKH DG++S FG D G+TWLTAFVL+SF A KY I+ ++ A +L + SDGCF ++G+LF++ M GG+ D +TAY+ SL ++ T T + ++ N ++ Q +F +L D ++S G +W +S+ S S D+E + Y +LA++ DL + + V WL Q+N+ GG+ STQDTVVAL AL+++A + S + V + +++ + DN+L+ Q L Y S++ GS C S+Q++ YNI A T + K E D ++ Q I + V Y ++ +NM +V I SG+ + S + + R+D + + +Y N A ++ ++++ + NL+PAVVK+ DYY SE YS +A
Sbjct: 541 DLTPVVQILAYCVLPSENVIA-----ANKTLDTEKCFGNKVSLQFSPAKAVPGEKNTLQLSAQPG---SLCGLSAVDQSVL----------------ILESGKRLEADKIFNLLPVHSVSDYPYSVEDGQACLYVRPRRSLLTDNTYETLKSVGLKMATNLAVRVPECLLYKGLAYHKNFVMYRQNAPAPGMFAQGMAGVDGLVGNSPSVTIRTVFPETWIWELAEVGDSGSAEVPVKVPDTITTWETEAFCLSSTGLGLAPPAQLTVFQPFFLELSLPYSIIRGEIFELKATVFNYLSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDIVTRSLLVQAEGIEKTETNSWLLCPKGDSLSEKVDLTLPKDVIEGSAKSSVSVIGDILGRALQNLHGLLRMPYGCGEQNMAVLSPNIYILQYLENTKQLTSAIREKASSFLKSGYQRQLNYKHFDGAYSTFGYGD--GNTWLTAFVLRSFGKAQKYTFIDPQIIQSAKDWLISRRDSDGCFIQQGRLFNNRMKGGVN-----DNVTMTAYITASLLELE-TPVTDPVITKGLSCLRSVIEEVKNTYTTALLAYTFSLARDTNTRQQLFNKLEDLAISDGPLVHW---SQSASADDSASLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSS--TVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKY-SIEVKGSACVSVQIAQFYNIPTPTEAK-TLSIDAKIEGDCEALGQNFIFDFTV--KYDGPEEKTNMAIVDIKLLSGFTADTSALGTSSGTYVSLVERVDSKDDHVIVYLKEIPKNVAMN---YQIQMKQVLPVKNLKPAVVKVYDYYQTSDQSEIEYSFHCEA 1443 HSP 2 Score: 83.1889 bits (204), Expect = 3.241e-15 Identity = 82/331 (24.77%), Postives = 138/331 (41.69%), Query Frame = 1
Query: 385 INETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINE-AMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC---MLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYS-KAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWG--FIRNYTSNKKGVIEF 1350
+ E R++ + + F +TDKPIY PG V R + +D N + + + +I++E + R+ QW NVS + Y+LN + G +K KA F G+ I+ F++KKYVLP+F V + P + +E + +VC+KY+ G+P+ K C + Y + C+ ++ K C + IL S+ + + K + VTE GT + +K+ E + Y P Y+ G G+I L P + + G NW + N T++ G+ F
Sbjct: 118 MTEERKVMFKSYDPLTFIQTDKPIYMPGQIVNFRV--------------------VTMDTNFAPVDQ---------QYSIIVLEDSQDNRIGQWTNVSSTRWILQRSYELNPECREGAYKLKA-FIGERMILHDFQVKKYVLPKFEVTLKKPKTVSVVEEELLIEVCAKYTYGQPVPGKSWVKVCRDPLPYIFFFPLCLGETLEITKTGCAIHNF--GLSMILNSTLKNNLKDSLHVEAVVTEEGTEITRTKS-ESVHLTYEIGKATLIDLPKTYEHGSVIKGKIKLFDFKDAPIPNKEVYL-----LEGENWSSKLLLNLTTDSDGLASF 410
BLAST of Alpha-2-macroglobulin vs. Ensembl Xenopus
Match: a2ml1 (alpha-2-macroglobulin-like 1 [Source:NCBI gene;Acc:100127688]) HSP 1 Score: 345.895 bits (886), Expect = 2.228e-96 Identity = 239/749 (31.91%), Postives = 384/749 (51.27%), Query Frame = 1
Query: 2650 IREYFPESWVFSY---FNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDAN-EINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICN-------KNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYW---PFDKESSDE--QKSNSADIETTGYAILALIN--HQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAK-TIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIR--NIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSC-FRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
IR YFPE+W++ ++ + PD++T W A ++CM G I+P L+ PF+ + +PYS+ E L VFNY C ++ + E ++ C C ++ KT Y+NLK +G++N+T+ + + + + + SD+V + +LV+P GV E + S LIC+ K E + + +P I+ GS R+Y+T GD+MG NLD+L+ MPYGCGEQNMV P+I++L YL + + +P + KAK ++ GY RQLTYK +DGS+SAFG D G+TWLTAFV+KSF A Y+ I+ L + +L+ ++ GCF GKLF++ M GG+ D L+AYV I+L L+ ++ A++C + + Y + + + + A+L +++V +W P K S ++ SA++E T YA+LAL++ ++DL + + V WLS Q+N GG+ STQDTVVAL ALA +A+ T +V + V+ ++ ++ + NRL+ Q +L Y S+ GS C +Q YNI ++ TF + V+T+ Q ++ +I + Y + SNM LV + SG+ + K L S + R + Q + LY N+ G D F +E+ ++ NL+PA VK+ DYY +E + + +N C+
Sbjct: 723 IRAYFPETWIWQLEPVGESGRAEVHGSAPDTITEWNAGAFCMGPSGFGISPPTSLRAFQPFFVELTLPYSVVRGESFTLKASVFNYLKQCIKVQTTLQPSLELEQEPCADCQYSSCICADESKTFYWNLKASNLGEVNITVRTEALNTQDMCNNEIPIVPKQGHSDTVIKPLLVQPGGVLIEKSHSSLICSPEGEDHSKTEDISL----NVPENILEGSERAYVTALGDLMGTAMQNLDRLLAMPYGCGEQNMVLFAPNIFILQYLENTHQLSPEIQTKAKGFLESGYQRQLTYKRNDGSYSAFGSRDPEGNTWLTAFVVKSFSKARPYIFIDGDHLNHSFSWLKDKRQRSGCFRSVGKLFNNAMKGGVD-----DEISLSAYVTIALMEAGLSKEDPMVSDALSCLRKAARKVSSAY-TQALLAYTFTLSDDTKHREKLLAKLEEKAVRKDGQLHWERKPASKASDLPYWYRAPSAEVELTSYALLALLSGPNKDLGKASEIVNWLSKQQNPYGGFSSTQDTVVALQALAKYAEATFSDKGDVTVTVTSKTGFHQQFH--VDQTNRLLLQKASLPDIPGEY-SLSATGSSCVYVQTVLRYNIPPP-RSDATFSVRVETQPKQCPQEPMKQMHIDIYIQYTGSREKSNMALVEVKMLSGFIPVKSTIKQLVS--SNTIKRSEIQTDMVTLY-----LNELGHDPVHFSIMVEQDIEVKNLKPATVKVYDYYE---TAEHAVTEYNSPCS 1447 HSP 2 Score: 71.633 bits (174), Expect = 1.711e-11 Identity = 54/214 (25.23%), Postives = 91/214 (42.52%), Query Frame = 1
Query: 640 PDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC---MLYSYGKKK-----CVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTS---SSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKP 1245
P+ R+AQW +V++ + + L L+ +P G + + + F +++YVLP+F V P + E K C +Y+ GKP+ C + Y +G CV F L + C+ + S+ + ++ + + T+TE GT S K + + SF D YKPG+PY G + +V N P
Sbjct: 166 PNKNRIAQWLDVTLTQGIAELSLPLSPEPSLGEYSIHV----GDTVHYFSVEEYVLPKFEVSFQFPKVVFFTSEQFQLKTCGRYTYGKPVQGTYKATVCRKVIRYWWGHNDDRSDLCVDFSGKLDQSGCSTLTATSENFNL---TYNTHEMGLEGTSTITEGGTGVEYSGSGKTIISSVVTSCSF---LDADSNYKPGIPYSGTVKVVDANDDP 369
BLAST of Alpha-2-macroglobulin vs. Ensembl Xenopus
Match: pzp (pregnancy-zone protein [Source:NCBI gene;Acc:779547]) HSP 1 Score: 342.043 bits (876), Expect = 3.274e-95 Identity = 281/998 (28.16%), Postives = 462/998 (46.29%), Query Frame = 1
Query: 1984 NETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFINTDNEVVADA--LEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKH-KCIPQKR-----VKRMIQPI-YGLEYDSRKVLSTAGFTAITNARIVN-VKCMQYLARG-----MP----DRS--YATFSNERTQM-SKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEIN-VKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELIC-------NKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQ-----KSNSADIETTGYAILALIN--HQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFA--YLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEK 4842
NE E+ + E I + L ++ P + V+ Y + ++ AD+ ++ N +G F+ +P + V L V + C +R+ D++++ + + + L D RI H P R KR IQP + E D + ITNA I V+C Y RG MP DR A+ S ER M + ++ + + + IR YFPE+W++ ++ + PD++T W A ++CM G I+P L+V PF+ + +PYS+ E L VFNY C ++ + + E+ ++ C C + KT Y+NLK +G++NVT+ + + + + + SD+V + +LV+P GV E + S L+C K E++ + +P I+ S R+Y T GD+MG NL +L+ MPYGCGEQNM P+I++L YL P + +KAK ++ GY RQLTYK +DGS+SAFG+ DK G+TWLTAFV+ SF A Y+ I+ S L + +L + GCF G+LF++ + GGI D L+ YV ++L ++ + A++C + + + Y + L + + A+L +++V +W + ++ SA++E T Y +LA ++ ++DL + + V WLS Q+N GG+ STQDTVVAL ALA + + + V+ + ++ +D NRL+ Q +L Y S+ GSGC +Q YNI ++ TF + V+TE D Q ++ +K+ Y + SNM ++ SG+ + L+ S + R + + LY D + F +E+ + NL+PA VK+ DYY+ E E++ + +N C +K
Sbjct: 479 NEDEVLRGEVS------IKLDLSADVSPTLRVLTYILLPGGDMPADSATFKLQRCFRNKVSVG-----FSPDEVLPGADVSLQVQAAAG-SLCGLRVVDQSVV-LMKPDKELTANKIHDLFPSRDFGNYDYRIQEYDDHCPFFPYWRPWNRFFKRSIQPWHFPEERDVYSLFKKMRIKIITNADIKKPVECQHYDFRGPGLPGMPGIPGDRRVVMASPSIERDVMVGAAMPDTIQQPEPEAAEIIRTYFPETWIWELAPVGESGRAEVHGSAPDTITEWNAGAFCMGPSGFGISPPTSLRVFQPFFVELALPYSVVRGESFTLKATVFNYLKQCIKVQTILLPSQELELEPCADCQYSSCLCAEESKTFYWNLKASNLGEVNVTVKTEALDTQDLCNNEFPIVPKQGRSDTVVKPLLVQPGGVLIEKSHSSLLCCQAGEDHPKTEEISL----KVPENILKDSERAYATVLGDLMGTAMQNLGRLLAMPYGCGEQNMALFAPNIFILQYLTKTRQLTPEVQSKAKGFLESGYQRQLTYKRNDGSYSAFGQRDKEGNTWLTAFVVNSFSKARPYIFIDESHLNHSFSWLRDNRNESGCFRSVGRLFNNALKGGID-----DETSLSTYVTMALLEAGVSVQDPVVADALSCLRKAAKDVSSVY-TQALLAYTFTLSKDIELREMLLAKLEEKAVRNEGQLHWERKPATVPSDLPYWYRAPSAEVELTSYVLLAHLSGPNKDLGKASEIVNWLSKQQNPYGGFSSTQDTVVALQALAKYGEATFTDKG---DVTATVTSKTGFHQQFHVDQTNRLLLQKASLPDIPGEY-SLSATGSGCVYVQTVLRYNIPPP-RSDATFSVRVETEPDKCPQDQLKVLKISMTAQYTGSREKSNMAVIECKMLSGF--IPVKSSVRKLEKSRTIKRSEILTEMVTLYLDELGHEPLH----LSFMVEQDIVVKNLKPATVKVYDYYDTE---EEAVAEYNSPCGSDGDK 1439 HSP 2 Score: 90.5077 bits (223), Expect = 2.921e-17 Identity = 74/305 (24.26%), Postives = 131/305 (42.95%), Query Frame = 1
Query: 376 GNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCM-LYSY--------GKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINV----TVTENGTSSSVSKAVEGPGIKYNSFNIKF-DGRPYYKPGLPYFGRINLVRHNKKP 1245
G I+ +R + + +F +TDK +YKPG VR R +++ NL E ++ P+ I ++ P R+ QW NVS+ + + L L+ +P G + + ++ + F +++YVLPRF V + P + + + +C++Y+ GKP+ C YSY G+ C F L + C+ + ++S +L S + Q+N+ +V E+GT +S + I + F D YKPG+ Y G + +V + P
Sbjct: 74 GETISNSRRVLVKRARTSLFVQTDKAVYKPGQTVRFRVVSLK-ENLQPEESQV-----------------PT-----------IELQDPGKNRIGQWLNVSLQQGIAELSLPLSSEPSLGEYSIRV----KDTVHGFSVEEYVLPRFEVTLQLPRVVAVNSKQFAVNICARYTYGKPVQGTYKVNVCRKHYSYRTMVASDQGRDLCADFSGTLSQSGCSTLDVQSSTYNLLLSEW-------QLNLEGTGSVLEDGTGIEISASGRS-QISSQLTTVTFLDADDTYKPGISYIGTVKVVDASDTP 337
BLAST of Alpha-2-macroglobulin vs. Ensembl Xenopus
Match: lrrc2 (leucine rich repeat containing 2 [Source:Xenbase;Acc:XB-GENE-962135]) HSP 1 Score: 295.434 bits (755), Expect = 1.736e-80 Identity = 255/932 (27.36%), Postives = 445/932 (47.75%), Query Frame = 1
Query: 2056 NMGPDVHVIAYFINTDNEVVADA--LEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSS--NADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIY--GLEYDSRKVLSTAGFTA-ITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTT---KHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEI--NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNK-NEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQ-------SVSFGRYKYWPFDKESSDE---QKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVC--FAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYY-DAFSYNDAGKDSCFRFKIEEKTKISNLQPAVV 4755
N+ P + + Y I EV+AD+ L++ N +L F+ A+P + L + + CA+R DE++L + +A Y + L L+ I + + L CI ++P++ G+ Y S ++ F ++ + + V+ + GM + RN + +++ ++ +R+YFPE+W++ L +TVPD++TTW+A +C ++ G ++ T L PF+ + +PYS E+ L VFNY + + + D+N+ ++ C N + T+ + + K +G++N T+ + + E + D++ + I+VE + + ++ +C++ E + + +P ++V GS R+Y + GD++G NL LV MPYGCGEQNMV P IY+ +YLN N P + +K SY+ GY +QL+YKH DGS+SAFG+ G+TWLTAF +KSF A ++ I + A +L Q+ +GCF GKLF++ M GG+ D L AY+ I+L L T + A+ C + Y AL +Y +L Q Q LD+ +V R ++ D D +++ SA++E Y +LAL ++ +DL+++ + V W+ Q+N GG+ STQDTVVAL AL+++ ++ +S + VS+ L++ + NRL+ Q L Y++ +GSGC +Q + YN+ + + F +SV T+ ++ + +++ + +Y+ + NSNM +V + PSG+ + ++ S RI F + E + + + D FRF +E++ I NLQPA V
Sbjct: 509 NIAPSANFLVYLILESGEVIADSISLKVEKCFGNKVEL-----SFSPSEALPGAPADLHLLTA-PSSLCALRAVDESVLILKPEAELSAQTIYNLLPLQDLSGYIHDGNFLEELWDDPCIK-------MEPLFINGIYYISCSFITGGPFAVPMSLSAGMGVQAGMGVQAGMSGAG----------LHIGRN--IPVAEPVIET-VRKYFPETWLWQLVETDADGKADLAVTVPDTITTWKAGMFCTSQGAGFGLSETISLVAFQPFFLELTLPYSAIRGEIFTLKATVFNYLSQTIRVGILLE-DSNQFLAKPTKVQEDGYCVTANGQVTLSWEVTLKSLGEVNFTMSAETLNGEGLCENEIVSPAQGRKDTITKHIIVEVGALWGKR--ADFLCSEITENIPL----KLPEKVVEGSARAYFSAVGDILGTAMQNLGNLVQMPYGCGEQNMVLFTPIIYISEYLNKTNQLTPEMKSKTLSYMSTGYQKQLSYKHPDGSYSAFGQRYGEGNTWLTAFTMKSFARARAHIYIEEKLITGALLWLSIRQKPNGCFHSVGKLFNNAMKGGVD-----DEVTLAAYITIALLEYPLPVTHPTVRNALFCLESALGGKNGIYTKALMAYAFTLAGRMDIRRQ--LLQSLDEQAIKKDGTVHLHRPEFS--DDSGVDRFPYRRAPSAEVEMNSYMLLALLSKHNVSDEDLTLATQVVSWMIKQQNPNGGFSSTQDTVVALQALSLYG-SLTLSHDAMRTVSLSLDKTPVAEFHVEDSNRLLLQRIALKEVPGDYLA-TVSGSGCLYLQTTLRYNV-PIPKEDAPFTISVTTDPETCDRKSLKTFTIVANVSYVGKRLNSNMAVVEVKLPSGY---------IPIKPSVKKVRISFIASGAEAHANEVILFKDI---QSFRFSVEQEIPIGNLQPATV 1383 HSP 2 Score: 104.375 bits (259), Expect = 1.574e-21 Identity = 103/401 (25.69%), Postives = 178/401 (44.39%), Query Frame = 1
Query: 358 LTKNITGNFIN--ETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLE-YQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDG-RPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQI----SEVSSNLKPPMAATTVYQ----SLTNFQSKDGHYLQFMPINDKFKC 1521
LT ++ G+ ++ R+I + +F +TDKPIYKPG KV+ R + LD+N +S+ + P R++QW NV + + + E +QL+ +P GI+K + + F +++YVLP++ V+V P + D+ I VC +Y+ GKP+ +I C +S C + C + +++ + S + + K + + T+ E+GT ++ AV IK + F +Y+ GLP FG++ L + P M N ++ I YG N+ YT+ + G EF+I + +S S+QI E S + + + YQ S+ F S+ YL+ PI +C
Sbjct: 97 LTLDVQGDALHFKSQRKILIKPLQNPVFIQTDKPIYKPGQKVQFRIAS--------------------LDENFHPVSE----------------KDPQGNRISQWLNVELKKGLTQESFQLSSEPTLGIYKLTVGREKSAPVTHSFSVEEYVLPKYEVQVKLPPVVGIMDKGIKVTVCGRYTYGKPVLGRIKVRVCRKFSDRYNLCKGEEDGAGPNGCFSEVVSTRMFQMRRSGY---EDKIRASATIIEDGTGVELTAAVSSE-IKKTLAKVSFRHLDSHYRKGLPLFGQLFLEDASGNP--MANETVVI-FVGYGTNF----TYTTGQDGTAEFLIDTSNVQKS-SLQIRARYKEGESCYQYRWVSVS-YQGDERSVKEFYSRAKSYLKIQPIYKILEC 448
BLAST of Alpha-2-macroglobulin vs. Ensembl Xenopus
Match: tubal3.1 (tubulin alpha like 3, gene 1 [Source:Xenbase;Acc:XB-GENE-490055]) HSP 1 Score: 293.123 bits (749), Expect = 8.697e-80 Identity = 258/962 (26.82%), Postives = 438/962 (45.53%), Query Frame = 1
Query: 2032 IISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSK--VVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSY-LVQKLIKLNHKITLDSR---IGLLRKHKCIPQKRVKRMIQPIY--GLEY---------DSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFS-NERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTT---KHLNLTVPDSMTTWRADSYCMTEK-GLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDE-------QKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVC--FAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+ +P+ N+ P ++ Y + EV+AD++ + + C + K F+ +P S+ + L +P + CA+R DE++L A+LS + L+ L D + LR C+P ++P+Y G+ Y D+ +L + G TN I K Y P+ + F E M+ +++ + +R+YFPE+W++ L +TVPD++TTW+A +C ++ G ++ T L PF+ + +PYS E L VFNY + + + + D L+ C N T+ +++ K +G++N T+ + + E + D++ + ILVEP GV E T + +IC K ++ +P +V GS R+Y + GD++G NL LV MPYGCGEQNMV P IY+ +YLN N P + +K SY+ G R L+ +F K LTAF +KSF A ++ I + +A +L Q+ +GCF GKLF++ M GG+ D L AY+ I+L L T + A+ C + Y AL +Y +L Q + L +Q++ +W + S D ++ SA++E T Y +L L ++ +DL+++ + V W+ Q+N GG+ STQDTVVAL ALA++ ++ +S++ V + L + + NRL+ Q L Y + +GSGC ++ + YN+ + F + V TE + + ++++ + +Y+ + NSNM L+ I PSG + + +++ + R + Q ++ +Y++ F F +E+ ISNLQPA I DYY + ++ YS
Sbjct: 417 LFDLPVGTNVSPSAKILVYLVLESGEVIADSITLK--VVQCFE-NKVKLSFSPTEGLPGSQAHLRLVTNP---TSLCAVRAVDESVLLL--KPEAELSARTIYDLLPLKDLSGYDHDGKFLEELRDDPCLP-------LEPMYINGIYYTPSNPDWDTDTYTILKSLGLKVFTNTNIRTPKLHFYFI-ACPNINLFYFGFTEELAMAPMAMRYMEVIET-----VRKYFPETWLWDLVETDADGKADLAVTVPDTITTWKAGMFCTSQSAGFGLSETISLVAFQPFFLELTLPYSAIRGEKFILKATVFNYLSQTIRVAISLEDSDQFLAKPSNLQEDGYCVKGNGRVTLSWDVTLKSLGEVNFTMSAETLTGEGLCENEIVDPIQGRKDTITKHILVEPEGVEKEETQNAMICGKGSEITEDIHLKLPERVVEGSARAYFSAVGDILGTAIQNLGNLVQMPYGCGEQNMVLFTPIIYISEYLNKTNQLTPEMKSKTLSYMSTG-KRDLSCTL----MCSFAK-------GLTAFTMKSFARARAHIYIEEKLISDALTWLSNHQKENGCFHSVGKLFNNAMKGGVD-----DEVTLAAYITIALLEYPLPVTHPTVRNALFCLESALGGKNSIYTKALMAYAFTLAGRMDIRRQ-LLQSLDEQAIKKDGTVHWQRPETSDDSGHYRNPYSQAPSAEVEMTSYGLLTLLSKPDVSDEDLTLATQVVSWIIKQQNPSGGFSSTQDTVVALQALALYG-SLTLSRDSTRTVLLSLEKTPVAKFNVEDSNRLLLQQIALKEVPGDYTA-TVSGSGCLYLKTALRYNVPHP-KEDAAFTILVTTEPTACDRKSLKSFAIVANVSYIGKRANSNMALMEIKMPSG-HVPVKSSVKKLPRLNNFIKRTEIQTNKVNVYFEL-------DIQSFSFLVEQDIPISNLQPATAIIYDYYEKDDFAVTKYS 1328 HSP 2 Score: 90.5077 bits (223), Expect = 2.353e-17 Identity = 91/386 (23.58%), Postives = 165/386 (42.75%), Query Frame = 1
Query: 427 IIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEY-QLNKDPIFGIWKAKAVFD-GQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASS-FRYSKAKSQINVT----VTENGTSSSVSKAVEGPG-IKYNSFNIKFDG-RPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSNLKPPMA--------ATTVYQSLT----NFQSKDGHYLQFMPINDKFKC 1521
++F +TDKPIYKPG KV+ R ++ LD+N +S+ + P R++QW NV + + + E+ QL+ +P G + + G F +++YVLP++ V+V P+ + + + VC +Y+ GKP+ +I+ C +S C + + ++ + L I ++ + F+ +A ++ + +TE+GT ++ EG IK + F +YK G+P +G++ L P M N ++ + Y N G YT+ + G EF I + S Q+S V + T Y+ T +F S+ +L+ PI+ +C
Sbjct: 38 LVFVQTDKPIYKPGQKVQFRIVS--------------------LDENFYPVSE----------------KDPQGNRISQWLNVELKKGLTQEFFQLSPEPTLGTYSLIVEREKGAPVTHSFSVEEYVLPKYEVQVKLPATLTILGQEVPVTVCGRYTYGKPVLGRINVRVCRKFSDRYNLCQGEEDGVCEEISQRADLNGCISDVVNTKVFQMRRAGYEMKIIASAKITEDGTGVELTG--EGSSEIKSTLATVSFRHLDSHYKKGIPLYGQVFLEDAAGNP--MSNETVVV----YVGNDGTNFTYTTGQDGTAEFSI------DTSSFQLSSVRIKVIYKTGDYCSGHRWLTASYEEDTRHVNHFYSRSKSFLKIQPIHRTLEC 373
BLAST of Alpha-2-macroglobulin vs. Ensembl Xenopus
Match: lrrc2 (leucine rich repeat containing 2 [Source:Xenbase;Acc:XB-GENE-962135]) HSP 1 Score: 290.426 bits (742), Expect = 8.658e-79 Identity = 216/729 (29.63%), Postives = 365/729 (50.07%), Query Frame = 1
Query: 2644 KQIREYFPESWVFSYFNKTT---KHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEI--NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNK-NEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQ-------SVSFGRYKYWPFDKESSDE---QKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVC--FAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYY-DAFSYNDAGKDSCFRFKIEEKTKISNLQPAVV 4755
+ +R+YFPE+W++ L +TVPD++TTW+A +C ++ G ++ T L PF+ + +PYS E+ L VFNY + + + D+N+ ++ C N + T+ + + K +G++N T+ + + E + D++ + I+VE + + ++ +C++ E + + +P ++V GS R+Y + GD++G NL LV MPYGCGEQNMV P IY+ +YLN N P + +K SY+ GY +QL+YKH DGS+SAFG+ G+TWLTAF +KSF A ++ I + A +L Q+ +GCF GKLF++ M GG+ D L AY+ I+L L T + A+ C + Y AL +Y +L Q Q LD+ +V R ++ D D +++ SA++E Y +LAL ++ +DL+++ + V W+ Q+N GG+ STQDTVVAL AL+++ ++ +S + VS+ L++ + NRL+ Q L Y++ +GSGC +Q + YN+ + + F +SV T+ ++ + +++ + +Y+ + NSNM +V + PSG+ + ++ S RI F + E + + + D FRF +E++ I NLQPA V
Sbjct: 716 ETVRKYFPETWLWQLVETDADGKADLAVTVPDTITTWKAGMFCTSQGAGFGLSETISLVAFQPFFLELTLPYSAIRGEIFTLKATVFNYLSQTIRVGILLE-DSNQFLAKPTKVQEDGYCVTANGQVTLSWEVTLKSLGEVNFTMSAETLNGEGLCENEIVSPAQGRKDTITKHIIVEVGALWGKR--ADFLCSEITENIPL----KLPEKVVEGSARAYFSAVGDILGTAMQNLGNLVQMPYGCGEQNMVLFTPIIYISEYLNKTNQLTPEMKSKTLSYMSTGYQKQLSYKHPDGSYSAFGQRYGEGNTWLTAFTMKSFARARAHIYIEEKLITGALLWLSIRQKPNGCFHSVGKLFNNAMKGGVD-----DEVTLAAYITIALLEYPLPVTHPTVRNALFCLESALGGKNGIYTKALMAYAFTLAGRMDIRRQ--LLQSLDEQAIKKDGTVHLHRPEF--SDDSGVDRFPYRRAPSAEVEMNSYMLLALLSKHNVSDEDLTLATQVVSWMIKQQNPNGGFSSTQDTVVALQALSLYG-SLTLSHDAMRTVSLSLDKTPVAEFHVEDSNRLLLQRIALKEVPGDYLA-TVSGSGCLYLQTTLRYNV-PIPKEDAPFTISVTTDPETCDRKSLKTFTIVANVSYVGKRLNSNMAVVEVKLPSGY---------IPIKPSVKKVRISFIASGAEAHANEVILFKDI---QSFRFSVEQEIPIGNLQPATV 1413 HSP 2 Score: 100.908 bits (250), Expect = 1.749e-20 Identity = 105/410 (25.61%), Postives = 178/410 (43.41%), Query Frame = 1
Query: 358 LTKNITGNFIN--ETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLE-YQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLE-------SKIVFILASSFRYSKAKSQI--NVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDG-RPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQI----SEVSSNLKPPMAATTVYQ----SLTNFQSKDGHYLQFMPINDKFKC 1521
LT ++ G+ ++ R+I + +F +TDKPIYKPG KV+ R + LD+N +S+ + P R++QW NV + + + E +QL+ +P GI+K + + F +++YVLP++ V+V P + D+ I VC +Y+ GKP+ +I C +S C + + C +I S++V R S + +I + T+ E+GT ++ AV IK + F +Y+ GLP FG++ L + P + I + YG N+ YT+ + G EF+I + +S S+QI E S + + + YQ S+ F S+ YL+ PI +C
Sbjct: 97 LTLDVQGDALHFKSQRKILIKPLQNPVFIQTDKPIYKPGQKVQFRIAS--------------------LDENFHPVSE----------------KDPQGNRISQWLNVELKKGLTQESFQLSSEPTLGIYKLTVGREKSAPVTHSFSVEEYVLPKYEVQVKLPPVVGIMDKGIKVTVCGRYTYGKPVLGRIKVRVCRKFSDRYNLCKGEEDGV----CEEINHRAGPNGCFSEVVSTRMFQMRRSGYEDKIRASATIIEDGTGVELTAAVSSE-IKKTLAKVSFRHLDSHYRKGLPLFGQLFLEDASGNPMANETVVIFV---GYGTNF----TYTTGQDGTAEFLIDTSNVQKS-SLQIRARYKEGESCYQYRWVSVS-YQGDERSVKEFYSRAKSYLKIQPIYKILEC 456
BLAST of Alpha-2-macroglobulin vs. Ensembl Mouse
Match: Mug1 (murinoglobulin 1 [Source:MGI Symbol;Acc:MGI:99837]) HSP 1 Score: 330.872 bits (847), Expect = 1.064e-91 Identity = 278/990 (28.08%), Postives = 469/990 (47.37%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADAL--EIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIG------------LLRKHK-CIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARI-VNVKCMQY--LARGMPDRSY-ATFSNE-----RTQMSKSRNSVLK---ISDAKMKKQIREYFPESWVFSYFNKTTKHL---NLTVPDSMTTWRADSYCMT-EKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINV-IGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKT--DKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXX------XXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQ------KSNSADIETTGYAILALINHQ------DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVF-AKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKT---EIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
+ IP+ +M P ++ Y I D EV+AD++ EI L N L +F+ ++P S+ L V ++ C +R D+++L S S++ +K SR+ L KH +P +G E D + + G TA TN I + + C Y + P + F+ E RT +SK + S+ + + IR+YFPE+WV+ + L +TVPD++T W+A + C++ + GL ++ L+ PF+ + +PYS+ E L V NY T +++V++ + V +G + C N T + + PK +G +N ++ + ++ SS + + D+V + ++VEP G+ E TFS L C + ++ +P +V S R++ + GD++ N L+ MPYGCGEQNMV P+IYVL YLN + KA ++ GY R+L YKH DGS+SAFG ++ G+TWLTAFVLKSF A ++ I+ S + A +L + Q+ +GCF G LF++ M GG+ D L+AY+ ++L L T ++KA++ E N + Y AL +Y +L + + + L ++++ +W ++S + +++SA++E Y +LA + Q DL++SM T+ WL+ Q+NS GG+ STQDTVVAL+AL+ + A T SQ L V + +++ ++ NRL+ Q L Y ++ +G GC Q YN+H L F + V+T ++ K N I + +Y SNM++ + SG+ + L+ ++R + + +Y D + + F F I++ + NLQPA+VK+ DYY + E +++ ++ C+
Sbjct: 518 LEIPVEFSMVPMAKMLIYTILPDGEVIADSVNFEIEKCLRNKVDL-----RFSTSQSLPASQTRLQVT-ASPQSLCGLRAVDQSVLLLKPESELSPSWIYNLPGMQQNKFVPSSRLSEDQEDCILYSSWLAEKHTNLVP-----------HGTEKDVYRYVEDMGLTAFTNLMIKLPIICFDYGMVPISAPRVEFDLAFTPEISWSLRTTLSKRPEEPPRKDPSSNDPLTETIRKYFPETWVWDIVTVNSTGLAEVEMTVPDTITEWKAGALCLSNDTGLGLSSVVPLQAFKPFFVEVSLPYSVVRGEAFMLKATVMNYLPTSMQMSVQLEASPDFTAVPVGDDQDSYCLSANGRHTSSWLVTPKSLGNVNFSVSAEAQQSSEPCGSEVATVPETGRKDTVVKVLIVEPEGIKQEHTFSSLFCASDAEISEKMSLVLPPTVVKDSARAHFSVMGDILSSAIRNTQNLLHMPYGCGEQNMVLFAPNIYVLKYLNETQQLTQKIKTKALGFLRAGYQRELNYKHKDGSYSAFGDQNGEREGNTWLTAFVLKSFAQARAFIFIDESHITHAFTWLSQKQKDNGCFRSSGSLFNNAMKGGVD-----DEMTLSAYITMALLESSLPATHPVVSKALSCLESSWKTIEQERNASFVYTKALMAYAFALAGNQNKRDE-ILKSLDEEAIKENNSIHWKRPQKSRKSEHHLYKPQASSAEVEMNAYVVLARLTAQPAPSPEDLTLSMSTIMWLTKQQNSNGGFSSTQDTVVALDALSKYGAVTFSRSQKTTL---VTIQSTGSFSQKFQVENSNRLLLQQVALPDIPGDY-TISVSGEGCVYAQTMLRYNMH-LEKQLSAFAIWVQTVPLTCNNPKGHNSFQISLEISYTGSRPASNMVIADVKMLSGF--IPLKPTVKKLERLEHVSRTEVSNNNVLIYLDQVT----NQTLAFSFIIQQDIPVRNLQPAIVKVYDYYETD---EMAFAEYSSPCS 1470 HSP 2 Score: 102.449 bits (254), Expect = 4.436e-21 Identity = 101/425 (23.76%), Postives = 174/425 (40.94%), Query Frame = 1
Query: 103 YFITSPNLLITETSHHFNVH--TSTDIVLTIESIHDPSQIKHQSSKFVIKKG-FQTIPYYVPFVGSHLGSK-IRLNILGDVCQ-SGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFD-GQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
Y + P+ L TET +H + V S+ S K+ + V+ K FQ + + +P + S + ++I G + S K L KN K ++F +TDKP+YKPG V+ R +++ ++R +E+ L IE P R+ QW+++ + + + L +PI G +K + G++E F + ++VLPRF V++ P+ + DE +S C KY+ GKP+ + C G C S L C+ E I + + Y +N TVTE GT S++ + + I +++ G+P+F ++ LV P + I Q +Y + T+++ G+ EF I
Sbjct: 31 YMVLVPSQLYTETPEKICLHLYQLNETVTVTASLVSQSGRKNLFDELVLDKDLFQCVSFIIPRLSSSDEEDFLYVDIKGPTHEFSKRKAVLVKN----------------KESVVFVQTDKPVYKPGQSVKFRVVSM--DKMLRPLNEL---------------------------LPLAYIEDPKKNRIMQWRDIKTENGLKQMSFSLAAEPIQGPYKIVVHKESGEKEEHSFTVMEFVLPRFNVDLKVPNAMSVNDEVLSVTACGKYTYGKPVPGHVKINVCRETETG---CREVNSQLDNNGCS--TQEVNITELQSKKRNYEVQLFHVNATVTEEGTGLEFSRSGTTKIERITNKLIFLKADSHFRHGIPFFVKVRLVDIKGDPIPNEKVFIKAQELSYTS------ATTTDQHGLAEFSI 399
BLAST of Alpha-2-macroglobulin vs. Ensembl Mouse
Match: Gm7298 (predicted gene 7298 [Source:MGI Symbol;Acc:MGI:3648717]) HSP 1 Score: 330.102 bits (845), Expect = 1.978e-91 Identity = 280/984 (28.46%), Postives = 471/984 (47.87%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADAL--EIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQ-------KLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVK-CMQY--LARGMPDRSY-ATFSNE-----RTQMSKSRNSVLK---ISDAKMKKQIREYFPESWVFSYF--NKT-TKHLNLTVPDSMTTWRADSYCMT-EKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSF--TLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKT--DKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXX------XXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYW--PFDKESSDEQ----KSNSADIETTGYAILALINHQ------DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVF-AKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKT---EIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKAC 4824
+ IP+ +M P ++ Y I D EV+AD++ EI L N L F+ ++P S+ L V ++ C +R D+++L + S++ K I +H + D +L C+ +K + +G E D + + G A TN +I + C Y + P + FS+E T +SK + S+ + IR+YFPE+WV+ N T + +TVPD++T W+A + C++ + GL ++ L+V F+ + PYS+ E L V NY T +++V++ D+ + + + + + C + T + + PK +G +N ++ + ++ S + + D+V + ++VEP G+ E TFS L+C + ++ +P +V S R++ + GDV+ N L+ MPYGCGEQNMV P+IYVL YLN + + KA ++ GY R+L YKH DGS+SAFG ++ G+TWLTAFVLKSF A ++ I+ S + + +L + Q+ +GCF+ G LF++ M GG+ D L+AY+ ++L L T ++KA++ E N + Y AL +Y +L + + L ++++ +W P S+ +++SA++E Y +LA + Q DL++SM T+ WL+ Q+NS GG+ STQDTVVAL+AL+ + A T SQ L V + + +++ ++ NRL+ Q L Y +M +G GC Q + YNIH L F L V+T + K N I + +Y SNM++ + SG+ + L+ P+ R + + LY D + + F F I++ + NLQPA+VK+ DYY + E +++ ++ C
Sbjct: 518 LEIPVEFSMVPLAKMLIYTILPDGEVIADSVNFEIEKCLRNKVDLS-----FSSSQSLPASQTRLQVT-ASPQSLCGLRAVDQSVLLLKPEAELSPSWIYNLPGMQHNKFISSSH-LPEDQEDCILCG-SCVAEKHTNSVP---HGREKDVYRYVEDMGLMAFTNLKIKHPTFCFDYDMVPILAPRVEFDLAFSHENTGSLHTVLSKRPEETPRKDPPSNDPFTETIRKYFPETWVWDIVTVNSTGVAEVEMTVPDTITEWKAGALCLSNDTGLGLSSVAPLQVFQDFFVEVSFPYSVVRGEAFMLKATVMNYLPTSMQMSVQLQ-DSPDFTAVPVGDYQDSYCLSAKERYTSSWLVTPKSLGNVNFSVSAEAQQSPEPCGSEVATVPETGRKDTVVKFLIVEPEGIKIEHTFSSLLCASDAEISEKLSLVLPPTVVKDSARAHFSVMGDVLSPAIKNTQNLLHMPYGCGEQNMVLFAPNIYVLKYLNETHQLTQTIKTKALGFLRAGYQRELNYKHKDGSYSAFGDQNGEREGNTWLTAFVLKSFAQARAFIFIDESHITHSLTWLSQQQKDNGCFQSSGSLFNNAMKGGVD-----DEMTLSAYITMALLESSLPATHPVVSKALSCLELSWKTIEQERNASFVYTKALMAYAFALVGNQDKRDE-ILKSLDEEAIKENNSIHWKRPQKPRKSEHHLYKPQASSAEVEMNAYVVLARLTAQPAPSPEDLTLSMSTIMWLTEQQNSNGGFSSTQDTVVALDALSKYKAVTFSRSQKTTL---VTIQSSGSFSQKFQVENSNRLLLQQVALPDIPGDY-TMSVSGEGCVYAQTTLRYNIH-LEKQPSAFALRVQTVPLTCNDPKGHNSFQISLEISYTGSRPASNMVIADVKMLSGF--IPLKPTVKKLESLAPVRRTEVSSNNVLLYLDQVNSHTLA----FSFIIQQDFLVRNLQPAMVKVYDYYETD---EVAFAEYSNPC 1469 HSP 2 Score: 98.9821 bits (245), Expect = 6.123e-20 Identity = 83/334 (24.85%), Postives = 146/334 (43.71%), Query Frame = 1
Query: 364 KNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWK-AKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKS-QINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
K T NF ++ + + +K ++F +TDKP+YKPG V+ R ++ +DKN + +++ F L IE P R+ QWK++ + + + L +PI G +K G +E F + ++VLPRF V + P+ I DE +S C+KY+ GKP+ + C G C S L C+ ++ + L R+ + + +N TVTE GT S++ + + I +++ G+P+F ++ LV P + I Q G+ T++++G+ +F I
Sbjct: 108 KGPTHNF-SKKEAVLVRNKESVVFVQTDKPVYKPGQSVKFRVVS--------------------MDKNLRPLNE---------LFPLAYIEDPKKNRIMQWKDIKTENGLKQISFSLAAEPIQGPYKIVLQKQSGVKEKHSFNVMEFVLPRFDVALKVPNAISVNDEVLSVTACAKYTYGKPVPGYVEISVCHETEAG---CKEVNSQLDNNGCSTQEVN---ITELQPKKRHHRVQLFHVNATVTEEGTGLEFSRSGTTKIERTTNKLIFLKADSHFRHGIPFFVKVLLVDIKGDPIPNEKVFIKAQ------ELGYTSATTTDQQGLAKFCI 399
BLAST of Alpha-2-macroglobulin vs. Ensembl Mouse
Match: A2ml1 (alpha-2-macroglobulin like 1 [Source:MGI Symbol;Acc:MGI:3039594]) HSP 1 Score: 298.13 bits (762), Expect = 2.419e-81 Identity = 263/973 (27.03%), Postives = 452/973 (46.45%), Query Frame = 1
Query: 2041 IPLMKNMGPDVHVIAYFINTDNEVVAD--ALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEA--LLQFGQSSNADLSYLVQKLIK----LNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIY-GLEY---------DSRKVLSTAGFTAITN-----ARIVNVKCMQYLARGM--PDRSYATFSNERTQMSKSRNSVLKISDAK--MKKQIREYFPESWVF---SYFNKTTKHLNLTVPDSMTTWRADSYCMT-EKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQC--KNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSN------SADIETTGYAILALINH--QDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVS-------VVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKT-EIDSVKQCNIR-NIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSM 4809
I + ++ P + Y ++ E+VAD L+I N + F++ +P S + + ++ CA+R D++ LL GQ + Y I + + LD + + CIPQK + Y GL Y D +LS G TN + + + Q L R P+ + S +SV IS AK +K+ +R FP +W++ S + T +++ VPD++T W A ++C+ G I+P L++ PF+ + P+S+ +E + V VFNY TC EI+V++ N I + T + ++KT + + PK +GK+NVT+ ++SK S C K+ ++ D+V + +LVE G+ E T S LIC K + ++ +PS +V GS+RS++T GD++G NL+ L+ MPYGCGEQN+ + +Y+LDYL + + L +KA+ + +GY L++K+ DGS+ F ++++ GSTWL+A K+ +Y+ I + K+ +L K Q+S+GCF K + G + D+ D LTAYV+ V L + + + C E +N N + ++++ + L + YW D++ + + S + E T Y +LA+++ QDL + K V WL+ + NS GG+ + QDT V L AL + K G + + +S +NRNK RL+ Q + + Y ++ G GC+ IQ + YN+ A+ F LSVKT + +S + + + V Y ++S +LV + SG+ + + L+ ++ + + D + + Y + N + + F IE+ ++N+QPA V + Y E Y+ SY++
Sbjct: 515 ISINADLAPSADLFVYTLHPSGEIVADNVRLQIEKCFKNKVSI-----NFSRDKDLPGSNTSVHLQ-AAPDSFCALRAVDKSALLLNHGQEMTPESVYFTLPYIHQYGYFYNGLNLDDQ----QAEPCIPQKDL------FYNGLYYTPTGNIWDGDLSNLLSNMGLKIFTNLHYRKPEVCSSQENQPLLRTFDHPNERIMMYGGGAPPSSAFHDSVDSISHAKVAIKETVRTNFPRTWIWNLVSVDSSGTANVSFLVPDTITQWEASAFCVNGNAGFGISPKVSLQISQPFFVEVTSPFSVVRSEQSDMVVTVFNYLTTCVEISVQLEASENYEASINTQRNTDSEVLQAGEQKTYVWTIIPKTLGKVNVTVVATSKQSRACPNDASKEQDVHWKDTVVKTMLVEAEGIEKEATQSFLICPKGTKASKQTLLELPSNVVEGSVRSFVTIVGDILGVAMQNLESLLQMPYGCGEQNIAQLASDVYILDYLKATDQLTEELKSKAQRLLSNGYQNHLSFKNYDGSYDVFCQSNQEGSTWLSALSFKTVEKMKEYIFIEETVPKQTLIWLVKKQKSNGCFRRDEKHVDTAQEG--REGDQED-IALTAYVVGVFLEVGLNASFPALRNGLYCLEEAFSNGVTNGYTQAILAYVFALAGKEQQAKSLLSILDKSATKTNNMIYWERDEKPETDNSPSFIPSALSGETEKTCYVLLAVLSQDTQDLDYASKIVQWLAQRMNSHGGFSAMQDTTVCLLALTQYMKLTGSNPQNTITLSSEESEEVFYVNRNK----------RLLVQHSKVSKGHQQY-TVDVEGDGCSFIQATLRYNVPLPKEASG-FSLSVKTGKSNSSDEFQTKFELTVTLTYTGARESSVTVLVDVKMLSGF--TPVVSSTEELKFNSQVTKTDIKNGHVLFYLE----NVPKEATSLTFSIEQTNHVANIQPAPVTVYSYEKGE-YAFDSYNI 1449 HSP 2 Score: 87.0409 bits (214), Expect = 2.240e-16 Identity = 77/334 (23.05%), Postives = 137/334 (41.02%), Query Frame = 1
Query: 385 INETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNV-SINEAMNLEYQLNKDPIFG----IWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKA----KSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGR-PYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
I+E R + + + F +TD P++KPGD V R + + N+ +P + + + LI ++ P + + QW NV + L +QL +PI G + K ++ G + F + + VLP+F VE+ P I D C+KY+ G+P+ K C + C + ++++ +Q ++K I F+ ++ +N VTE+GT +SK I + F+ P+Y+ G+ YFG + N P + + + G NYT+++ G F I
Sbjct: 106 ISERRSVAIAAEENATFVQTDTPVHKPGDTVHFRVVTL---NIWLKPVD--------------------------DLYPLITVQDPQSNVIFQWINVTTFRNITQLSFQLTPEPILGDYTIVIKTQS---GTTVMDHFTVNRDVLPKFEVELTAPETITIADSQFQMVTCAKYTYGQPVQGKAQIKVCREL-FSPAHCESNENEICEQFTVQLKDGCASHIINTKVFQLDRSGLFMTLNVNEVVTESGTGVQMSKT-HSVFITSVLGTVSFENMDPFYRRGITYFGTLKFSGPNNTPLVDKLLQLELDGKPVG-------NYTTDENGEARFSI 398
BLAST of Alpha-2-macroglobulin vs. Ensembl Mouse
Match: Cd109 (CD109 antigen [Source:MGI Symbol;Acc:MGI:2445221]) HSP 1 Score: 281.952 bits (720), Expect = 2.554e-76 Identity = 260/957 (27.17%), Postives = 448/957 (46.81%), Query Frame = 1
Query: 2029 RIISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKK--QIREYFPESWVF--SYF-NKTTKHLNLTVPDSMTTWRADSYCMTEK---GLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKIN--------VDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELI--CNKNEQLGILSV-FAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNN-TPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKES-SDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVV---LNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNI--------HRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSY 4803
R S+ + P +IAY+I D E++ D L+I L +++ K ++K T P KV L + ++ I D+++ S++ + +V +L N + L G+ +S V G +T+A ++ R D Y T ++ +++ DA +R+ FPE+W++ +Y +K + +TVPDS+T+W A ++ ++E GL P + L+ PF+ + +PYS+ E L V + NY ++ + I + +N+ N + T+ +++ T+ F +KP +G++ +T+ ++S SD+V + I+V+P G+ + S L+ + N + S+ F+ P + V GS R +T GD++G + + L L+ MPYGCGEQNM+ P+IY+LDYL L KA SY+ GY R+L Y+ +DGSFSAFG D GSTWL+AFVL+ F +A Y++I+ L +L ++ +G F E G++ S + GG K LTAY++ S+ G I++ +I + + NY L +L T S ++ L+ +S G ++W + S + S DIE YA+LA H +S + + WL QRNSLGG+ STQDTVVAL AL+ F+ + +N +++++V + R+ + I N + L + V++ +GSG A QL+ YN+ R + F L V +++ + N+ VC ++L ++ + M+L+ +N SG+ S S+ +S L ++++ ++ LY D S N++ C K+SN++ V ++DYY P + +SY
Sbjct: 517 RTFSLTPEASWAPKACIIAYYIAEDGEIINDILKIPVQLVFENKV---KLFWSKPTVKPSDKVSLRISATQSDSLVGIVAVDKSVTLMENSNSITMETMVHELELYNTEYYL--------------------------GMFMNSFAVFQECGLWVLTDATLI---------RDSIDEVYDTEEYSERFAEENEANLVDFEDASSVNNVHVRKNFPETWIWLDAYMGSKIYEEFEVTVPDSITSWVASAFVISEDLGFGLTTVPAE-LQAFQPFFLFLNLPYSVIRGEEFALEVSIVNYLKDTIKVVILIEESDSFDILMTSNDTNGTIYRK-TVQVPRDNGVTLVFPIKPTHLGEIPITVTAASP---------------TASDAVTQTIVVKPEGIEKSYSKSVLLDLTDSNVESKQQSMRFSFPPDTVIGSERVQITAIGDILGSSINGLSSLIRMPYGCGEQNMIYFAPNIYILDYLTKQKQLTVNLKEKALSYMRQGYQRELLYQREDGSFSAFGDIDSSGSTWLSAFVLRCFLEADYYIDIDQDVLHRTYTWLNAHKKFNGEFWEPGRVIHSELQGGTK-----SPVTLTAYIVTSVLGYKKYQPNIDVQDSIKFLEFEFSRGISDNY--TLAIISYALSTVGSPKAEEALNLLMQRSEKEGDTQFWLSSGPALSGSWQPRSVDIEIAAYALLAHTLHH-VSEGIPVMRWLIQQRNSLGGFVSTQDTVVALKALSEFSALVH-KENTDIQLTVTGPGIPRSIHFR--IDSQNLFLLHQEELHALDPITVNVSAHGSGFAICQLNVDYNVKGSGSSKRRRSTENQEVFDLDVI--VNNEDDISHLNLNVCTSHLGSER-TGMVLMEVNLLSGF-----SASSDSIPLSETLKKVEYDNGKLNLYLD--SVNES--QFCVNIPTVRDYKVSNIRDGSVSVMDYYEPRRQAVRSY 1395 HSP 2 Score: 67.3958 bits (163), Expect = 2.210e-10 Identity = 102/443 (23.02%), Postives = 181/443 (40.86%), Query Frame = 1
Query: 382 FINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSIN-EAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCV------------AFKSDLMKQNCNKIKLESKIVFILASSFRYSK------AKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIK-FDGRPYYKPGLPYFGRINLVRHNK---KPAKMVNISISIQSWAYGNN--------WGFIR--NYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSNLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIV 1611
F N TR + K+ + +TDK YKP +V+ R + + ++L KP + D I I+ P + + QW + + ++ +QL+ +PIFG W + + Q+ F++ +YVLP+F V V P K + ++ V +KY+ GKP+ +S F L +GKKK + +F + MK+ N L S Y ++I TVTE+ T S A K + + I+ FD KP L + + + R + P ++ N +++ + N+ +I+ NYT + G+I+ P + ++ L ++ + ++ TV+ + F S Y+Q ++ K +L + N S V S+GQ+V
Sbjct: 115 FSNRTR-LTFESKSISVLIQTDKAFYKPKQEVKFRVLTLC-SDL-----------------------KPYRTSVD------IFIKDPKSNVIQQWFSQKGDLGVVSKTFQLSSNPIFGDWSIQVQVNDQQYYQSFQVLEYVLPKFEVTVQTPLYCSLKSKQLNGSVIAKYTYGKPVKGSLSLTFLPLSFWGKKKNITKSFEINGFANFSFDNYEMKKVMNLKPLTD------VSEGSYENVDPSFPGPAEIIATVTESLTGIS-RMASTNVFFKQHDYIIEIFDYTTVLKPSLNFTATVKVSRSDGNQLTPEEIENDLVTVVTQRKNNHPESQRDQEMDYIQTVNYTIPQNGIIKIEFPVMSISGELQLKAYFLDGT-----SSVTVH---SMFTSPSKTYIQLKTRDEYIKVGSPFDLMVSGNRQFKDLSYMV--ISKGQLV 509
BLAST of Alpha-2-macroglobulin vs. Ensembl Mouse
Match: Pzp (PZP, alpha-2-macroglobulin like [Source:MGI Symbol;Acc:MGI:87854]) HSP 1 Score: 263.462 bits (672), Expect = 1.821e-70 Identity = 183/621 (29.47%), Postives = 307/621 (49.44%), Query Frame = 1
Query: 2032 IISIPLM--KNMGPDVHVIAYFINTDNEVVADA--LEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSR---IGLLRKHK-CIPQKRVKR---MIQPIYGL-EYDSRKVLSTAGFTAITNARIVNVK-CMQY---------------LARGMPDRSYATFSNERTQM--SKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTK---HLNLTVPDSMTTWRADSYCMT-EKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINV-IGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGK---TDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL 3777
+ S+P+ M P+ ++ Y I + E+VADA EI N L F ++P S L V + CA+ D+++L A LS Q + L T+ + + + H+ CI + + + P + L + D+ + + G TN++I + C ++ +A P S+ + S N V+++ ++ +R+YFPE+W++ L + VPD++T W+A ++C++ GL ++ T L+ PF+ + +PYS+ E L V NY C +I V + + + + V +G + C C N+ KTV + + PK +G++N T + + S K ++ + D+V + ++VEP G+ E T++ L+C ++ +L +P +V GS R+ + GD++G NL L+ MPYGCGEQNMV VP+IYVL+YLN A+ +KA +Y+I GY RQL Y+H DGS+S FG + G+TWLTAFVLK+F A ++ I + + A +L Q+ +GCF++ G L ++ M GG+ D L+AY+ I+L
Sbjct: 522 VFSLPIQVEPGMAPEAQLLIYAILPNEELVADAQNFEIEKCFANKVNL-----SFPSAQSLPASDTHLKVK-AAPLSLCALTAVDQSVLLL--KPEAKLS--PQSIYNLLPGKTVQGAFFGVPVYKDHENCISGEDITHNGIVYTPKHSLGDNDAHSIFQSVGINIFTNSKIHKPRFCQEFQHYPAMGGVAPQALAVAASGPGSSFRAMGVPMMGLDYSDEINQVVEV-----RETVRKYFPETWIWDLVPLDVSGDGELAVKVPDTITEWKASAFCLSGTTGLGLSSTISLQAFQPFFLELTLPYSVVRGEAFTLKATVLNYMSHCIQIRVDLEISPDFLAVPVGGHENSHCICGNERKTVSWAVTPKSLGEVNFTATAEALQSPELCGNKLTEVPALVHKDTVVKSVIVEPEGIEKEQTYNTLLCPQDTELQDNWSLELPPNVVEGSARATHSVLGDILGSAMQNLQNLLQMPYGCGEQNMVLFVPNIYVLNYLNETQQLTEAIKSKAINYLISGYQRQLNYQHSDGSYSTFGNHGGGNTPGNTWLTAFVLKAFAQAQSHIFIEKTHITNAFNWLSMKQKENGCFQQSGYLLNNAMKGGVD-----DEVTLSAYITIAL 1122 HSP 2 Score: 108.997 bits (271), Expect = 5.307e-23 Identity = 86/283 (30.39%), Postives = 139/283 (49.12%), Query Frame = 1
Query: 4009 EQKSNSADIETTGYAILALINHQ-----------DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVF-AKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKT---EIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+ ++ SA++E T Y +LA + + DLS + K V W+S Q+NS GG+ STQDTVVAL AL+ + A T SQ +V V + + T++ ++ NRL+ Q L +YV+ K +GSGC +Q S YNI + F L V T D I++ +Y +SNM++V + SG+ + K L Q + + R + + +Y + + G F F +E+ + NL+PA +K+ DYY + ++ + YS
Sbjct: 1211 QPRAPSAEVEMTAYVLLAYLTSESSRPTRDLSSSDLSTASKIVKWISKQQNSHGGFSSTQDTVVALQALSKYGAATFTRSQK---EVLVTIESSGTFSKTFHVNSGNRLLLQEVRLPDLPGNYVT-KGSGSGCVYLQTSLKYNILPVADGKAPFALQVNTLPLNFDKAGDHRTFQIRINVSYTGERPSSNMVIVDVKMVSGFIPMKPSVKKLQDQPN--IQRTEVNTNHVLIYIEKLTNQTLG----FSFAVEQDIPVKNLKPAPIKVYDYYETDEFTVEEYS 1483 HSP 3 Score: 92.0485 bits (227), Expect = 6.270e-18 Identity = 81/328 (24.70%), Postives = 142/328 (43.29%), Query Frame = 1
Query: 430 IFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMN-LEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKK---------CVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGT--------SSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
+F +TDKPIYKPG V+ R ++ V+I N+ F ++ IETP R+ QW+N+ + ++ L + L+ +P GI+K D ++I FE+K+YVLP+F V + + +E + C Y+ GKP+ ++ C YS + C F + C + +++K VF L + K ++ + E GT S ++ A+ +K+ N Y+PGLP+ G++ LV KP NI+ + + G++ +T+++ G+ I
Sbjct: 127 VFVQTDKPIYKPGQIVKFRVVS----------------VDISFRPLNE-------------TFPVVYIETPKRNRIFQWQNIHLAGGLHQLSFPLSVEPALGIYKVVVQKDSGKKIEHSFEVKEYVLPKFEVIIKMQKTMAFLEEELPITACGVYTYGKPVPGLVTLRVCRKYSRYRSTCHNQNSMSICEEFSQQADDKGCFRQVVKTK-VFQLRQ--KGHDMKIEVEAKIKEEGTGIELTGIGSCEIANALS--KLKFTKVNTN------YRPGLPFSGQVLLVDEKGKPIPNKNITSVV------SPLGYLSIFTTDEHGLANISI 408
BLAST of Alpha-2-macroglobulin vs. UniProt/SwissProt
Match: sp|Q63041|A1M_RAT (Alpha-1-macroglobulin OS=Rattus norvegicus OX=10116 GN=A1m PE=1 SV=1) HSP 1 Score: 355.525 bits (911), Expect = 1.272e-98 Identity = 290/993 (29.20%), Postives = 472/993 (47.53%), Query Frame = 1
Query: 2032 IISIPLM--KNMGPDVHVIAYFINTDNEVVADA--LEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALL----QFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKR---MIQPIYGL-EYDSRKVLSTAGFTAITNARIVNVK-CMQYLA---------------RGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFN---KTTKHLNLTVPDSMTTWRADSYCMT-EKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINV-IGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGK---TDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXX---XXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQN-VFAQLLDQSVSFGRYKYWPFDK---ESSDEQKS-------NSADIETTGYAILAL-----------INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVF-AKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKT---EIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
++S P+ M P +I Y I + E++AD +I N L F ++P S LTV + CA+ D+++L + S + + L QK + + L + G CI + + + P L + D+ V + G TN R+ + C Y A +P Y + SN RT + + ++++ +R+YFPE+W++ L + VPD++T W+A ++C++ GL ++ T KV PF+ + +PYS+ E L V NY C I+V + + + + V +G + C C N+ KTV + + PK +G++N T + + S K ++ + D+V + ++VEP G+ E T++ L+C ++ +L +P+ +V GS R+ + GD++G NL L+ MPYGCGEQNMV VP+IYVL+YLN A+ +KA SY+I GY RQL Y+H DGS+S FG G+TWLTAFVLK+F A Y+ I + + A +L QR +GCF++ G L ++ M GG+ D L+AY+ I+L + L T + A + A +I+N+ ++ + + ++ V L +V+ +W K E+ E +S SA++E T Y +LA ++ DL+ + K V W+S Q+NS GG+ STQDTVVAL AL+ + A T S N +VSV + + T +G + ++ NRL+ Q L +Y++ K +GSGC +Q S YNI F L V T D + I + +Y+ NSNM++V + SG+ + K L Q + + R + + +Y + + G F F +E+ + NL+PA VK+ DYY + ++ + YS
Sbjct: 522 VVSFPIRVEPGMAPVAKLIVYTILPNEELIADVQKFDIEKCFANTVNLS-----FPSAQSLPASDTHLTVK-ATPLSLCALTAVDQSVLLLKPEAKLSPQSIYNLLPQKAEQGAYLGPLPYKGG----ENCIKAEDITHNGIVYTPKQDLNDNDAYSVFQSIGLKIFTNTRVHKPRYCPMYQAYPPLPYVGEPQALAMSAIPGAGYRS-SNIRTSSMMMMGASEVAQEVEVRETVRKYFPETWIWDMVPLDLSGDGELPVKVPDTITEWKASAFCLSGTTGLGLSSTISHKVFQPFFLELTLPYSVVRGEAFILKATVLNYMPHCIRIHVSLEMSPDFLAVPVGSHEDSHCICGNERKTVSWAVTPKSLGEVNFTATAEALQSPELCGNKVAEVPALVQKDTVVKPVIVEPEGIEKEQTYNTLLCPQDAELQENWTLDLPANVVEGSARATQSVLGDILGSAMQNLQNLLQMPYGCGEQNMVLFVPNIYVLEYLNETQQLTEAIKSKAISYLISGYQRQLNYQHSDGSYSTFGDRGMRHSQGNTWLTAFVLKAFAQAQSYIYIEKTHITNAFNWLSMKQRENGCFQQSGSLLNNAMKGGVD-----DEVTLSAYITIALLEMPLPVTHSVVRNALFCLETAWASISNSQESHVYTKALLAYAFALAGNRAKRSEVLESLNKDAVNEEESVHWQRPKNVEENVREMRSFSYKPRAPSAEVEMTAYVLLAYLTSASSRPTRDLSSSDLTTASKIVKWISKQQNSHGGFSSTQDTVVALQALSKYGAATFTKS---NKEVSVTIESSGTVSGTLHVNNGNRLLLQEVRLADLPGNYIT-KVSGSGCVYLQTSLKYNILPEAEGEAPFTLKVNTLPLNFDKAEHHRKFQIHINVSYIGERPNSNMVIVDVKMVSGFIPVKPSVKKLQDQ--SNIQRTEVNTNHVLIYIEKLTNQTMG----FSFAVEQDIPVKNLKPAPVKVYDYYETDEFAIEEYS 1488 HSP 2 Score: 103.605 bits (257), Expect = 1.474e-20 Identity = 81/321 (25.23%), Postives = 144/321 (44.86%), Query Frame = 1
Query: 364 KNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMN-LEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYS---------YGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPG---IKYNSFNIKF-DGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQS 1281
K T +FI + + + + +F +TDKPIYKPG V+ R ++ V+I N+ F ++ IE P R+ QW+NV + ++ L + L+ +P GI+K D ++I FE+K+YVLP+F V+V P + +E + C Y+ GKP+ ++ C Y+ + K C F ++ C + +++K+ + K ++ + E+GT ++ G G I +KF +Y+PGLP+FG++ LV +P N+++ + S
Sbjct: 106 KGPTHHFIKK-KSMWITKAESPVFVQTDKPIYKPGQTVKFRVVS----------------VDISFRPVNE-------------TFPVVYIENPKRNRIFQWQNVDLPGGLHQLSFPLSVEPALGIYKVVVQKDSGKKIEHSFEVKEYVLPKFEVQVKMPKTMAFLEEELVVTACGLYTYGKPVPGLVTMKVCRKYTQSYSNCHGQHSKSICEEFSKQADEKGCFRQVVKTKVFQPRQKGY---DMKIEVEAKIKEDGTGIELT----GTGSCEIANTLSKLKFTKANTFYRPGLPFFGQVLLVDEKGQPIPNKNLTVQVNS 389
BLAST of Alpha-2-macroglobulin vs. UniProt/SwissProt
Match: sp|A8K2U0|A2ML1_HUMAN (Alpha-2-macroglobulin-like protein 1 OS=Homo sapiens OX=9606 GN=A2ML1 PE=1 SV=3) HSP 1 Score: 339.732 bits (870), Expect = 1.005e-93 Identity = 280/984 (28.46%), Postives = 458/984 (46.54%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVD--PGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLL------------RKHKC-------IPQKRVKRMIQ-----------PIYGLEYDSRKVLSTAGFTAITNARIVN-VKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSF-----TLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPF----DKESSDEQKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGV-SQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAAN-DTFKLSV---KTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPL-NRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+S+ + PD ++ Y I VVAD ++ + + +Q+ G F+ +P ++V L + PG + CA+R DE++L L++ + +++ S G+ +C PQ + M Q P + D G ++NA+I V C + P+ Y+T ++ S + A+ Q+R+YFPE+W++ F N + +++TVPD++T W+A S+C ++ +G ++PT L PF+ ++ +PYS+ E +L +FNY C +++ D + + L+S+ + C C +D KT ++N+ +G +N TI + S+ +K + SD++ + +LV+P GV E T S L+C K + +P +IV S ++Y+T GD+MG NLD LV MP GCGEQNMV P IYVL YL + + ++A ++ GY ++L YKH +GS+SAFG+ D G+TWLTAFV K F A K++ I+ +++A K++ Q GC+ G L + M GG+ D LTAYV +L G D+ + +N +T N + + + QL Q++ G YW +S + + D+E T YA+LA + ++++ + V WL+ Q N+ GG+ STQDTVVAL ALA +A T + S+ +NL V N +T+N I NRLV Q L Y +++ +G GC +Q YNI L N TF LSV K + + + +Y+ +SNM +V + SG+ E + L Q PL +++F + +Y D N + F I + ++NL+PA +K+ DYY P+ + YS
Sbjct: 520 LSLTFTSRLAPDPSLVIYAIFPSGGVVADKIQFSVEMCFDNQVSLG---FSPSQQLPGAEVELQLQAAPG---SLCALRAVDESVL----------------LLRPDRELSNRSVYGMFPFWYGHYPYQVAEYDQCPVSGPWDFPQPLIDPMPQGHSSQRSIIWRPSFSEGTDLFSFFRDVGLKILSNAKIKKPVDC----SHRSPE--YSTAMGAGGGHPEAFESSTPLHQAE-DSQVRQYFPETWLWDLFPIGNSGKEAVHVTVPDAITEWKAMSFCTSQSRGFGLSPTVGLTAFKPFFVDLTLPYSVVRGESFRLTATIFNYLKDC----IRVQTDLAKSHEYQLESWADSQTSSCLCADDAKTHHWNITAVKLGHINFTISTKILDSNEPCGGQKGFVPQKGRSDTLIKPVLVKPEGVLVEKTHSSLLCPKGKVASESVSLELPVDIVPDSTKAYVTVLGDIMGTALQNLDGLVQMPSGCGEQNMVLFAPIIYVLQYLEKAGLLTEEIRSRAVGFLEIGYQKELMYKHSNGSYSAFGERDGNGNTWLTAFVTKCFGQAQKFIFIDPKNIQDALKWMAGNQLPSGCYANVGNLLHTAMKGGVD-----DEVSLTAYVTAALLEMGKDV---DDPMVSQGLRCLKNSATSTTNLYTQALLAYIFSLAGEMDIRNILLKQLDQQAIISGESIYWSQKPTPSSNASPWSEPAAVDVELTAYALLAQLTKPSLTQKEIAKATSIVAWLAKQHNAYGGFSSTQDTVVALQALAKYATTAYMPSEEINLVVKSTENFQRTFN--IQSVNRLVFQQDTLPNVPGMY-TLEASGQGCVYVQTVLRYNI--LPPTNMKTFSLSVEIGKARCEQPTSPRSLTLTIHTSYVGSRSSSNMAIVEVKMLSGFSPMEGTNQLLLQQ---PLVKKVEFGTDTLNIYLDELIKN----TQTYTFTISQSVLVTNLKPATIKVYDYYLPDEQATIQYS 1450 HSP 2 Score: 90.5077 bits (223), Expect = 1.565e-16 Identity = 81/322 (25.16%), Postives = 139/322 (43.17%), Query Frame = 1
Query: 433 FAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCM---LYSYGK-------KKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINV--TVTENGTSSSVSKAVEGPGIKYNSFNIKF-DGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
F +TDKP+Y PG +V R + +D N F+ ND + ++ ++ P++ R+AQW V + + +L +QL + + G + AV +G+ F +++YVLP+F VEV+ P E+ ES K+C +Y+ GKPM + C Y Y + KC K C ++ ++ ++ + QIN+ TV E GT + A + I ++ F D +Y P P+ G+I + H+ K + + I YG N F + ++ G+ F +
Sbjct: 123 FVQTDKPLYTPGQQVYFRI--------------------VTMDSN--FVPV---NDK----YSMVELQDPNSNRIAQWLEVVPEQGIVDLSFQLAPEAMLGTYTV-AVAEGKT-FGTFSVEEYVLPKFKVEVVEPKELSTVQESFLVKICCRYTYGKPMLGAVQVSVCQKANTYWYREVEREQLPDKCRNLSGQTDKTGCFSAPVDMATFDLIGYAYSH-----QINIVATVVEEGTGVE-ANATQNIYISPQMGSMTFEDTSNFYHPNFPFSGKIRVRGHDDSFLKNHLVFLVI----YGTNGTFNQTLVTDNNGLAPFTL 403
BLAST of Alpha-2-macroglobulin vs. UniProt/SwissProt
Match: sp|P01023|A2MG_HUMAN (Alpha-2-macroglobulin OS=Homo sapiens OX=9606 GN=A2M PE=1 SV=3) HSP 1 Score: 334.724 bits (857), Expect = 4.349e-92 Identity = 276/984 (28.05%), Postives = 455/984 (46.24%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADAL--EIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYL-VQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEY---------DSRKVLSTAGFTAITNARIVNVK----CMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINV-IGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKT--DKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXX-----XXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSD------EQKSNSADIETTGYAILALINHQ------DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNAL-----AVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAAND-TFKLSVKT---EIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNK 4830
ISIP+ ++ P ++ Y + +V+ D+ ++ + L N L F+ ++P S L V ++ CA+R D+++L +A+LS V L+ + CI + V I G+ Y D L G A TN++I K QY G P+ F E M + ++ + + + +R+YFPE+W++ + + +TVPD++T W+A ++C++E GL I+ T L+ PF+ + +PYS+ E L V NY C ++V++ + V + + C C N +TV + + PK +G +N T+ + + S + + D+V + +LVEP G+ ETTF+ L+C ++ +P +V S R+ ++ GD++G N L+ MPYGCGEQNMV P+IYVLDYLN P + +KA Y+ GY RQL YKH DGS+S FG+ G+TWLTAFVLK+F A Y+ I+ + + +A +L + Q+ +GCF G L ++ + GG++ D L+AY+ I+L + LT T + A T ++ + + + V L +++V +W ++ E ++ SA++E T Y +LA + Q DL+ + V W++ Q+N+ GG+ STQDTVVAL+AL A F +T +Q V + + T++ +D NRL+ Q +L Y SMK G GC +Q S YNI L + F L V+T D K I + +Y SNM +V + SG+ + L+ S ++R + + +Y D S + F + + + +L+PA+VK+ DYY + E + + +N C+K
Sbjct: 526 ISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDL-----SFSPSQSLPASHAHLRVT-AAPQSVCALRAVDQSVLLM--KPDAELSASSVYNLLPEKDLTGFPGPLNDQDNEDCINRHNVY-----INGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHG-PEGLRVGFY-ESDVMGRGHARLVHVEEPHTET-VRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVE-----DEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQ-------VTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEY-SMKVTGEGCVYLQTSLKYNI--LPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGF--IPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVS----NQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETD---EFAIAEYNAPCSK 1469 HSP 2 Score: 90.5077 bits (223), Expect = 1.495e-16 Identity = 80/330 (24.24%), Postives = 154/330 (46.67%), Query Frame = 1
Query: 415 DKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKA--KAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYS-----YGKKK---CVAFKSDLMKQNCNKIKLESKIVFILASSFR---YSKAKSQINVTVTE--NGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
+++ ++F +TDK IYKPG V+ R ++ +D+N +++ L+ I+ P R+AQW++ + + + L+ +P G +K + G+ E F ++++VLP+F V+V P I +E ++ VC Y+ GKP+ ++ C YS +G+ C F L C ++++K+ + + +++A+ Q TV E SS +++ I SF +K D +++ G+P+FG++ LV + K + N I I+ GN + N T+++ G+++F I
Sbjct: 124 NEDSLVFVQTDKSIYKPGQTVKFRVVS--------------------MDENFHPLNE---------LIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEH-PFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRT-----ITKLSF-VKVDS--HFRQGIPFFGQVRLV--DGKGVPIPNKVIFIR----GNEANYYSNATTDEHGLVQFSI 409
BLAST of Alpha-2-macroglobulin vs. UniProt/SwissProt
Match: sp|Q5R4N8|A2MG_PONAB (Alpha-2-macroglobulin OS=Pongo abelii OX=9601 GN=A2M PE=2 SV=1) HSP 1 Score: 334.724 bits (857), Expect = 4.628e-92 Identity = 277/986 (28.09%), Postives = 456/986 (46.25%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADAL--EIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKH---KCIPQKRVKRMIQPIYGLEY---------DSRKVLSTAGFTAITNARIVNVKC----MQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINV-IGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKT--DKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXX-----XXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSD------EQKSNSADIETTGYAILALINHQ------DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNAL-----AVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLD--NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAAND-TFKLSVKT---EIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNK 4830
ISIP+ ++ P ++ Y + +V+ D+ ++ + L N L F+ ++P L V ++ CA+R D+++L +A+LS + L + L G L CI + V I G+ Y D L G A TN++I K QY G + ++ +R L ++ + +R+YFPE+W++ + + +TVPD++T W+A ++C++E GL I+ T L+ PF+ + +PYS+ EV L V NY C ++V++ + V + + C C N +TV + + PK +G +N T+ + + S + + D+V + +LVEP G+ ETTF+ L+C ++ +P +V S R+ ++ GD++G N L+ MPYGCGEQNMV P+IYVLDYLN P + +KA Y+ GY RQL YKH DGS+S FG+ G+TWLTAFVLK+F A Y+ I+ + + +A +L + Q+ +GCF G L ++ + GG++ D L+AY+ I+L + LT T + A T ++ + + + V L +++V +W ++ E ++ SA++E T YA+LA + Q DL+ + V W++ Q+N+ GG+ STQDTVVAL+AL A F +T +Q V + + T++ +D NRL+ Q +L Y SMK G GC +Q S YNI L + F L V+T D K I + +Y SNM +V + SG+ + L+ S ++R + + +Y D S + F + + + +L+PA+VK+ DYY + E + + +N C+K
Sbjct: 526 ISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDL-----SFSPSQSLPALHAHLRVT-AAPQSLCALRAVDQSVLLM--KPDAELS--ASSVYNLLPEKDLTGFPGPLNDQGDEDCINRHNVY-----INGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKLCPQLQQYEMHGPEGLRVGFYESDVMGRGHAR---LVHAEEPPTETVRKYFPETWIWDLVVVNSSGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEVFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAITPKSLGNVNFTVSAEALESQELCGTEVASVPEYGKKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVE-----DEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLQSLHEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYALLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQ-------VTIQSSGTFSNKFQVDNNNRLLLQQVSLPELPGEY-SMKVTGEGCVYLQTSLKYNI--LPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGF--IPLKPTVKMLERSNHVSRTEVSNNHVLIYLDKVS----NQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETD---EFAIAEYNAPCSK 1469 HSP 2 Score: 89.3521 bits (220), Expect = 2.961e-16 Identity = 79/329 (24.01%), Postives = 151/329 (45.90%), Query Frame = 1
Query: 415 DKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVF-DGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYS-----YGKKK---CVAFKSDLMKQNCNKIKLESKIVFILASSFR---YSKAKSQINVTVTE--NGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
+++ ++F +TDK IYKP V+ R ++ +D+N +++ L+ I+ P R+AQW++ + + + L+ +P G +K G+ F ++++VLP+F V+V P I +E ++ VC Y+ GKP+ ++ C YS +G+ C F L C ++++K+ + + ++KA+ Q TV E SS +++ I SF +K D +++ G+P+FG++ LV + K + N I I+ GN + N T+++ G+++F I
Sbjct: 124 NEDSLVFVQTDKSIYKPAQTVKFRVVS--------------------MDENFHPLNE---------LIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGRRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASNCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTKAQIQEEGTVVELTGRQSSEITRT-----ITKLSF-VKADS--HFRQGIPFFGQVRLV--DGKGVPIPNKVIFIR----GNEANYYSNATTDEHGLVQFSI 409
BLAST of Alpha-2-macroglobulin vs. UniProt/SwissProt
Match: sp|P20740|OVOS_CHICK (Ovostatin OS=Gallus gallus OX=9031 PE=1 SV=3) HSP 1 Score: 331.643 bits (849), Expect = 5.210e-91 Identity = 274/954 (28.72%), Postives = 456/954 (47.80%), Query Frame = 1
Query: 2041 IPLMKN--MGPDVHVIAYFINTDNEVVADALE--IASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSS--NADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKR---MIQPIY-GLEYDSRKVLSTAGFTAITNARIVN-VKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAK--MKKQIREYFPESWVFS--YFNKTTK-HLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKS-----NSADIETTGYAILALI-----NHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTI--GVSQNVNLKVSVVLNRNKTYNGLITL--DNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSV--KQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSY 4803
IPL+ N M P + ++ Y ++ E+VAD++ I N QL +F++K + S V L ++ + CA+R D+++L + +A+ Y + + L I + + C+ + +P+ GL D + L G TN++I C + R A F+ T K V + K + + IRE+FPE+W++ N T K ++ T+PD++T W+A ++C+ E G ++ L PF+ ++ +PYS+ E + VFNY C +INV + + + C C K+ +N+ PK G + +I + + C E+ + I I D+ R +LVEP G+ E T + LIC K++ + +P+ +V GS R + GD+MG N+ +L+ MP+G GEQNMV P+IYVLDYL+ + + +K Y++ GY +QL+YKH DGS+S FG DK G+TWLTAFV KSF +AS+++ I+ + + +L Q++DGCF+ G L ++ M GG++ L+AY+ I+L + + I A C N + + + ++ +L + KYW ++ S+ E+ S D+E T Y +LAL+ + +DL+ + V W+ Q+NS GG+ S QDTVVAL ALA + V+QNV + +N T+ + T+ +NRL+ Q L Y S+ NG+GC IQ + YNIH L F LSV+T S Q +I + +Y + +SNM+++ + SG+ + L + + +++++K + LY + F +E+ +++ +PA V+I DYY E Y+ Y
Sbjct: 532 IPLVVNEKMAPALRLLVYMLHPAKELVADSVRFSIEKCFKNKVQL-----QFSEKQMLTTSNVSLVIEAAAN-SFCAVRAVDKSMLLLKSETELSAETIYNLHPIQDLQGYIFNGLNLEDDPQDPCVSSDDIFHKGLYYRPLTSGLGPDVYQFLRDMGMKFFTNSKIRQPTVCTRETVRPPSYFLNAGFT-ASTHHVKLSAEVAREERGKRHILETIREFFPETWIWDIILINSTGKASVSYTIPDTITEWKASAFCVEELAGFGMSVPATLTAFQPFFVDLTLPYSIIHGEDFLVRANVFNYLNHCIKINVLLLESLDYQAKLISPEDDGCVCAKIRKSYVWNIFPKGTGDVLFSITAETNDDEACEEEALRNIRIDYRDTQIRALLVEPEGIRREETQNFLICMKDDVISQDVAIDLPTNVVEGSPRPSFSVVGDIMGTAIQNVHQLLQMPFGNGEQNMVLFAPNIYVLDYLDKTRQLSEDVKSKTIGYLVSGYQKQLSYKHPDGSYSTFGIRDKEGNTWLTAFVYKSFAEASRFIYIDDNVQAQTLIWLATKQKTDGCFQSTGILVNNAMKGGVENELS-----LSAYITIALLEAGHSMSHTVIRNAFYCLETASEKNITDIYTQALVAYAFCLAGKAEICESFLRELQKSAKEVDGSKYWEQNQRSAPEKSHLLDHVQSTDVEITSYVLLALLYKPNRSQEDLTKASAIVQWIIRQQNSYGGFASMQDTVVALQALAAYGAATYNSVTQNV-----IKINSKNTFEKVFTVNNENRLLLQQTPLPQVPGKY-SLTVNGTGCVLIQTALRYNIH-LPEGAFGFSLSVQTSNASCPRDQPGKFDIVLISSYTGKRSSSNMVIIDVKMLSGFVPVKSSLDQLIDDHT--VMQVEYKKNHVLLYLGNILQK---RRKEVTFSVEQDFVVTHPKPAPVQIYDYYETEEYAVAEY 1461 HSP 2 Score: 109.768 bits (273), Expect = 2.111e-22 Identity = 90/340 (26.47%), Postives = 155/340 (45.59%), Query Frame = 1
Query: 385 INETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVS--INEAMNLEYQLNKDPIFGIWKAKAVF-DGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLY-SYGKKK----CVAFKSDLMKQNCNKIKLESKIVFILASSFRYS-------KAKSQI-NVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
+ E R + + + +F +TDKPIYKPG V R +A LD N K + + + LI ++ P N R+ QW+NV+ IN + +E+ L ++PI G +K G+ F +++YVLP+F V V P + D ++ K+C+ Y+ G+P+ K+ C + SYG+ K C +F DL C L SK+ + ++ + K Q+ N+T T++ + + V +++ + + +Y+ G+PYFG+I LV + P + IQ + N N+T++ G+ F I
Sbjct: 125 LKERRSVMIWNMESFVFVQTDKPIYKPGQSVMFRVVA--------------------LDFNFKPVQE---------MYPLIAVQDPQNNRIFQWQNVTSEIN-IVQIEFPLTEEPILGNYKIIVTKKSGERTSHSFLVEEYVLPKFDVTVTAPGSLTVMDSELTVKICAVYTYGQPVEGKVQLSVCRDFDSYGRCKKSPVCQSFTKDLDTDGCLSHILSSKVFELNRIGYKRNLDVKAIVTEKEQVCNLTATQSISITQVMSSLQFENVDH-----------HYRRGIPYFGQIKLVDKDNSPIS----NKVIQLFVNNKN---THNFTTDINGIAPFSI 416
BLAST of Alpha-2-macroglobulin vs. TrEMBL
Match: A0A4Y6HTR9 (Alpha-2-macroglobulin OS=Dugesia japonica OX=6161 PE=2 SV=1) HSP 1 Score: 2542.69 bits (6589), Expect = 0.000e+0 Identity = 1221/1855 (65.82%), Postives = 1516/1855 (81.73%), Query Frame = 1
Query: 85 FAVKDFYFITSPNLLITETSHHFNVHTSTDIVLTIESIHDPSQIKHQSSKFVIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSNLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSGGCLNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPITKNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGM--PDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYEWNLELEPKNCSCLNSWESFNKSFVILSSRWRSLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYYSVIKNITEWYCANFTDWKLNISSTSPIVKGVLSSNVSSIPVELEFPHQFCYCYEHVRDTKKSFRIVDLNHKLQSLNQKPKDPIKLMLNKGFEVVDEEIFSSLKNIKCG 5643
F +KDFYFIT+PNLL+ E H FNVHTSTD+VLTI+SIH PS+I HQSS ++I+KGF +IPYYVPF+GSH+GSKI LN LGD C +KC+ N+TGNFINETREIGLIDKNFIIFAETDKPIYKPG+K++ RFM IYPTNLMREP++IG Q EI+L+KNNKF K+ N S F+LI IE P RVAQW NVS+ +AM+L YQL DP+FG WK+KA FDGQEE+IQFE+K+YVLPRF+V V PP EILA +SI FKVCSKYSTGK M A+I G FCM ++ + C FKS L+KQ+CN+I +++K +F L S R+++ KS INVTV ENGTSS VSK VEG GI Y SF IKF + YYKPGLPYFG++ LVRHN KPA +N+SI +++WAYG WGF R YTSNK+G+++F++P IPL QS++++++EVS+++K PM+A T+YQS+ N++S D +Y+QF+P+N K KCP LNLT++TNYDI+ ++YVQIFSRG+I MIEYPS+PVKC+PR+DFFGHY+CVNGT L CL GWKGGNCREPIC GGCLNGACVEPNKCICK YW G+ C+QC A YCKNGYC NG DC+CYPGW +FDCSKKGP H Y N +T+ P + +TEI+KFEKQT+FERII++PL+KNMGPDVH+I YFI +NEVVAD++E+++GL NCS LGGGKFKF++KT +PK+KVVLT+DPGVK++ C++RIAD AL QFG+SSN DL+YL+QKLI LNHKIT+D+R K C+ + RVK+ I P Y YDSRKVL+T+GFTAITNARI+N KC+ + ARG DR AT+ V K ++ K+IRE FPESW+F+YFNKT K LNLTVPDSMTTW+AD+YCMTE+GLWIAPTQKLKVILPF++NIYVPYS+KL+E L +PVK+FNYA C+EI VKINV++ EINVIGLKSFTLC C NDEKT+YF ++PKIIGKLN+T+DS SKYS+FCSE + K NI MSDS+RRQ+LVEP GVPTETTFSELICNKNEQLGI++VF IP+ IV GSLRSY+TFNGDVMG+TFDNLDKLV MPYGCGEQNMVAVVPSIYVL+YL +L I NP L +KA+ YII+GY RQL YKH+DGSFSAFGK+DK GSTWLTAFVL+SF+ A+KYV +N S L +A KYLEKMQ+++GCF+E GKLFSSTMAGG+K+ND D LLTAYVLISL D+ + TI I KAI C T NI+ N NYRLAL++Y LS+Y SS+ ++NV QL Q+ GRY+YW FDK++ +E K+ S+DIETTGYA+LA+ N Q L S+KT+YWLSNQRNSLGGYYSTQDTVVAL+ LA FA+TIG+S NVNL +S+ LN K YN LIT DNRLVS+TFNLG ENISY+S+KTNG+GC S+QL SLYNI +LY+A +TFKLSVK EI SV+QCNIR IK+CF+YLRRDK+SNMIL+IINYPSGWEL+E+E+KS+ LQI+TPLNRIDFQK++MELYYDAFSYNDAGKDSCFRF++EEK+KISNLQP+++K++DYYNP+IY+E SYSMFNK CN SD K P P+VLLCPICVNDS+ ST+ FI +IC +WF GFVT + + + R ++ ++QS F EW L++EP NCSCL +W++ N SF++LSS+W+ L+EKL LSNDS+M+P+ TLYS++++DLQ QC +A N Y+M NRL+PDFANKT +N+SCP CIL +NYY I+N+T WYCAN+TDW L IS SPI+ G+L +N+SSI VE+EF ++FCYC+E +RD KKSFRIVDL +++ ++ K P KL+LN +EV++ +F K+IKCG
Sbjct: 35 FGIKDFYFITTPNLLLVEAPHQFNVHTSTDLVLTIDSIHTPSKINHQSSNYIIQKGFDSIPYYVPFIGSHVGSKITLNFLGDFCSDLLKCKSVTNVTGNFINETREIGLIDKNFIIFAETDKPIYKPGEKIKFRFMPIYPTNLMREPEDIGDQKEIILNKNNKFEWVLVKDKNMSQIFELIFIENPTGARVAQWINVSLAKAMDLRYQLTNDPVFGTWKSKATFDGQEELIQFEVKRYVLPRFVVTVNPPKEILANQKSIGFKVCSKYSTGKTMKAQIHGTFCMHHTDKTRNCEYFKSTLLKQSCNRIDIDTKSIFNLKSKSRFTRTKSVINVTVIENGTSSVVSKVVEGSGIMYTSFAIKFVSKLYYKPGLPYFGQLKLVRHNNKPASNINVSIDLENWAYGQGWGFHRIYTSNKEGIVDFIVPNIPLEQSVTVRVNEVSNDIKAPMSAATLYQSIANWESVDKYYMQFLPLNSKVKCPDDLNLTIVTNYDISEINMYVQIFSRGRITDMIEYPSIPVKCFPRNDFFGHYECVNGTVLVCLPGWKGGNCREPICVGGCLNGACVEPNKCICKAYWKGENCDQCIIAPYCKNGYCKNGNDCKCYPGWQSFDCSKKGPKVHSYENYSTIMPTSVKQTEIKKFEKQTVFERIITVPLLKNMGPDVHIIIYFITPNNEVVADSIELSNGLKNCSHLGGGKFKFDRKTTVPKTKVVLTLDPGVKDSSCSVRIADIALSQFGKSSNVDLAYLIQKLINLNHKITVDNRFSFGTKRLCVSKDRVKKSIWPGYRSGYDSRKVLATSGFTAITNARIINAKCLLFAARGSVNKDRVVATY-------------VQKTLKTEVNKKIRENFPESWLFAYFNKTNKLLNLTVPDSMTTWKADAYCMTERGLWIAPTQKLKVILPFFANIYVPYSIKLDEQLSVPVKIFNYASACYEIYVKINVESEEINVIGLKSFTLCVCSNDEKTLYFKIQPKIIGKLNITVDSISKYSTFCSEDQLKT-NIEMSDSIRRQLLVEPSGVPTETTFSELICNKNEQLGIVNVFTIPTTIVPGSLRSYVTFNGDVMGQTFDNLDKLVKMPYGCGEQNMVAVVPSIYVLEYLTNLKIVNPILISKAEGYIINGYNRQLLYKHEDGSFSAFGKSDKAGSTWLTAFVLRSFNAANKYVKLNSSMLTDAVKYLEKMQQNNGCFKENGKLFSSTMAGGVKKNDNTDEALLTAYVLISLK--DIKSKTIKIDKAIKCITANISQNPSNYRLALSAYALSIYQNSSNATENVIQQLRTQATEIGRYRYWLFDKDAMEESKTTSSDIETTGYALLAMQNSQSLFDSLKTIYWLSNQRNSLGGYYSTQDTVVALSVLATFAETIGLSNNVNLNLSITLNNEKNYNDLITTDNRLVSRTFNLGAENISYISIKTNGTGCVSVQLISLYNIQKLYSAKETFKLSVKPEIISVEQCNIRKIKICFSYLRRDKDSNMILLIINYPSGWELTEDEEKSVKLQITTPLNRIDFQKSKMELYYDAFSYNDAGKDSCFRFQLEEKSKISNLQPSLIKLMDYYNPDIYTETSYSMFNKDCNSSDVKIPEIPKVLLCPICVNDSLESTVNFIQSICQSWFQGFVTDDEDGIKNRIIYESKQSFFNEWKLDIEPMNCSCLKTWKNLNNSFIVLSSKWKYLSEKLILSNDSVMYPINTLYSSFKYDLQNQCRMAKNLYMMMNRLLPDFANKTEQNNSCPSCILGSNYYFKIQNLTSWYCANYTDWNLTISYLSPIITGILKNNISSINVEIEFSNRFCYCFEQIRDLKKSFRIVDLKNQIPVIDNKSNKPFKLLLNNDYEVIENNVFLWNKHIKCG 1873
BLAST of Alpha-2-macroglobulin vs. TrEMBL
Match: A0A5J4NQR5 (Alpha-2-macroglobulin OS=Paragonimus westermani OX=34504 GN=DEA37_0001242 PE=4 SV=1) HSP 1 Score: 624.78 bits (1610), Expect = 0.000e+0 Identity = 491/1689 (29.07%), Postives = 779/1689 (46.12%), Query Frame = 1
Query: 418 KNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIG---KQVEIVLDKNNKFIS-KPSKND--NDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCML--------------------------YSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVN-----ISISIQSWA-------YGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQIS-----------EVSSNLKPPMAAT--------TVYQSLTNFQSKDGHYLQFMPINDKF--KCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKM---IEYPSVPVKCYPRDDFFGHYQCVNGTT--LTCLSGWKGGNCREPICSGGC--LNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGP--IKHLYRNVTTVKPITKNETEIRKFEK-----------QTIFERIISIPLMKNMGPDVHVIAYFINTDN-----EVVADALEIASGLNNCSQLGGG--------KFKFNKKTAMPKSKVVLTVDP----------GVKENG------CAIRIADEALLQFGQSSNA-DLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITN------ARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKH--------LNLTVPDSMTTWRADSYCMTEK-GLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVF-----------NYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI--------NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVF--AIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLN---SLNIKNPA-LSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSF---HDASKYVNINLSTL-KEATKYLEKMQRSD--GCFEEKGKLFSSTMAGGIKRNDKADGK-LLTAYVLISLNG------------VDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQ-----SVSFGRYK-YWPFDKESSDE--QKSNSADIETTGYAILALINHQDLSVS--MKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGV----SQNVNLKVSVVLNR-NKTYNGLITLDNRLVSQTF----------NLGTENISYVSMKTNGSG-CASIQLSSLYNIHRLYAAND-TFKLSV---KTEIDSVKQCNIRNIKVCF-----AYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRME------LYYDAFSYNDAGKDS-------CFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSM 4809
+N ++ ETDKPIY+PG+ VR+RF+A+ P + + + ++ I LD N F+ P+ D N Y ++ ++++ DN RV QW S A NL Y L D G W AK F G+ E+++F +K+YVLPRF+ + PP+ + + + F VC+KY+ G+PM + C+ S+ + C+ L C ++ + F + + + V E GT S+V++ G + +I+ P +KPGLP G + V +P +V+ I ++ + +A + + F+ + ++ G F IP I + L ++ + EVS +LKP + Y + + S+ G LQ P + KCPG + L ++ N +A +IYVQ +RG++++ E V C RDD GHY+CV+ T+ + CL GW G +C P+C+ GC G C +PN C C WTG C +C K CK G CVNG DC C PGW+ F C + ++ + ++ T + E R+ E+ +T++ER I+ + + GP+ V+ YF N DN E+V +E+ + L+NC+ F++ P +V LTV P VKE C +R++D +L F Q N D++ ++++ + I + H +P ++ ++ + FT I AR+ + +A +P + S+ + NSV + ++R++FPE W+F Y +TT+H NLTVPDS+TTWRA ++C T+ GLWI P+++L V +PF++ I +P + E+L LP+ + N + CFEI V VD + + + FT C C+ +++T L P+ +G+LNVT+ + + S + + + + D VRR + V P GVP + ++IC + +L VF + +V GSLR Y ++ +V+G NL+ LV MP GCGEQNMV V P++YVLDYL S+N++ L A++YI GY Q Y+ +DGS+SAFGK+D+ GSTWLTAFVL F H + I +TL E YL + Q S GCF EKG++ + + GG+ + LLTAYVL +L G V+ + ++ N Y LA +Y SL P SS + + +LL++ S K +W S + + + DIETT +A LAL LS+S + WL++Q+N+ GG+ STQDTV+ L +A AK +G+ + L+V V+L+ + + +TL+ R V F L NI S N SG C ++Q YN+ AA F LS+ + K C + VC A+++RD S M+L+ I SGW+ E+E L + P + + M LY+DAF+ ++A C K+++ + N + AVV +DYY E ++Y +
Sbjct: 141 RNVVVLGETDKPIYRPGEVVRMRFLALKPQFTVPLNETVSYPLRKPIITLDGNVTFVELTPADVDMLNGIEYEEISLVDSQDN-RVKQWLRASPRLAANLSYHLLNDAPEGEWHAKVHFSGKTEVLRFSVKQYVLPRFLTIIQPPNNLTFETKFSQFSVCAKYTDGQPMLGFVRAQLCVCERSSDGKITLDEQNSITTENICPKNHASFKPRPCIRLNELLRADGCAFFNTTTRPLEFDNIEFPH-WGQGLVCAEVEEEGTQSTVTRCELGASVSKKKASIELTIPPVFKPGLPIMGSV--VLRGVEPVHLVHGAELTIKVTEERFACWYPRFSVKDTYQFVTSVKTDSNGSARFYIPPINSTRRLFVKATYVPGDPNNTFEEVSHDLKPHSLLSFTSRNKEIEAYDFIRPWLSQSGSSLQLWPTDKPVVAKCPGVVTLNMIANTQLAKRAIYVQCIARGRLLQRYIPAELVEVSEMCRDRDDHLGHYKCVSTTSDRIVCLPGWVGNDCLHPVCANGCHPRGGVCYQPNTCTCIDGWTGLNCQECVKRTNCKQGRCVNGDDCVCDPGWVGFLCDTRKVHFVETVNADIQTNENNESPEKTFRELEEPDKTTEAVFSHRTLYERQINFSIDGDWGPEATVVVYFYNGDNDSRGVEIVPMVIELRN-LDNCTNPATAMQAESLVSGINFDQNHVSPGQQVQLTVRPVDQTFNPVTRAVKEREQIPDQVCFLRMSDISLDNFEQEKNVIDMTSFLKRIQQTQETI---------KWHSAVPDNVLE-------AFRSAGLQLTTLTDFTTIQQTFQPCLARMYSTVVDGPMASALPAPPMSLISSNAVTKEQEANSV----PIQTMPRLRDFFPEVWLFDYAPVEQQTTQHGQSFYGVQTNLTVPDSITTWRASAFCTTKSNGLWIPPSKRLTVSMPFFAEITLPKQVIRGEILYLPISIHVIDNEIKSAGDNGSKECFEIRVFTQVDEQDWVRVSISEFTGCACRGEKRTFQMGLLPRRLGQLNVTVIAQATSGSVICDSDTDDMLFKVNQEKHRMIQDMVRRSVRVVPEGVPRISAIGDIICLRAPELSKTQVFPLVMTENVVPGSLRVYWSYTDEVLGPALKNLNSLVQMPTGCGEQNMVLVAPNVYVLDYLKSSPSVNVRQTEKLIRSARTYIEAGYHGQSKYRLEDGSYSAFGKSDRVGSTWLTAFVLSVFSKAHRVDSSIRIEWTTLFNETVDYLIQHQDSQGAGCFVEKGRVIHTAIQGGLSVGTSVSKESLLTAYVLAALWGTRPIVGQTLSSRVNTSVNDGIRCLTEAIHPGSVINQMSTYELAQFAYTFSLIKPQSSDTVALIKELLNRRHSETSAGLADTKNFWSIRSNLSTNYPRMAEAIDIETTSFAYLAL-TQSGLSISELFPVIRWLASQQNANGGFRSTQDTVLGLEVIADGAKRLGLHTLNTTENQLEVEVLLDPLGYSISDTVTLEKRRVVNQFAVPYTEPHQAQLARWNI---SAPLNSSGQCLAVQTVLYYNVPDTDAAGQAAFDLSINVAQPRAPPAKACTTAAVTVCLRVPERAHMQRD--SGMLLIKIPMVSGWQPIEKEVFGLMS--AGPRDSELVRMVEMSDDGTLWLYFDAFTSSEATSAGGWSNLKRCAEVKLKQNVYVENTRSAVVTAMDYYATERSISRNYGL 1796
BLAST of Alpha-2-macroglobulin vs. TrEMBL
Match: A0A1S8WUB0 (A-macroglobulin complement component OS=Opisthorchis viverrini OX=6198 GN=X801_06124 PE=4 SV=1) HSP 1 Score: 583.178 bits (1502), Expect = 2.812e-171 Identity = 514/1892 (27.17%), Postives = 820/1892 (43.34%), Query Frame = 1
Query: 106 FITSPNLLITETSHHFNVHT-----STDIVLTIESIH----DPSQIKHQSSKFVIKKGFQTIPYYVPFVG-SHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAI-------------YPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCML--------------------------YSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQ---SW-------------AYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSN---------LKPP------MAATTVYQSLTNFQSKDGHYLQFMPINDKFKCP--GKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYP--SVPVKCYPRDDFFGHYQCVNGTT--LTCLSGWKGGNCREPICSGGC--LNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCS----KKGPIKHLYRNVTT-----VKPITKNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFIN---TDNEVVADALEIASGLNNCS-------QLGGGKFKFNKKTAMPKSKVVLTVDPG----------------VKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYG----LEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSY-----FNKTTKH------LNLTVPDSMTTWRADSYCMTEK-GLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVF--------NYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELIC--NKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNS----LNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKY---VNINLSTL-KEATKYLEKMQ--RSDGCFEEKGKLFSSTMAGGI-KRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENIT----------------NNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSF-----GRYKYWPFDKESSDEQKS---NSADIETTGYAILALINHQDLSVS-MKTVYWLSNQRNSLGGYYSTQDTVVALNALA------VFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTE--NISYVSMKTN-----GSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDS----VKQCNIRNIKVCFAYLRRD---KNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKT-RMELYYDAFSYNDAGKDS-------CFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKS--DEKEP-----------------GRPEVLLCPIC-VNDSINSTLKFI--GTICNAWFNGFVT----ANSNASQRRTVFVAEQSMFYEWNLELEP---KNCSC 5055
FIT P+ L E + +V T S ++ +I S++ D Q+ S+KF Q I Y PFV + + ++LN C + E T+ + +TR + + I+ ETDKPIY+PG+ VR RF+ + YPT + G E+ LD F S+ ++ Y ++ + + N R+ +W NVS EA L Y L D G W A G+ E + F +K +VLPRF+VE+ PP + + + VC++Y+ G PM + C+ S+ + CV L K C K ++ + + +R + V E GT S+V++ G + ++ YKPGLP I++ P + + I+++ SW + + + G + VIP +S++ +N + PP Y L+ + S G +Q P F G + + LL N + ++ VQ RG++VK I P V C RDD GHY+C T + CL GW+G +C +PIC+ GC G C P C C++ WTG C C K + C +G CV+G DC C GW C + I+ + V+T V + TE ++T++ R IS P+ + GP+ VI YF + E++ LE+ N S Q +F++K P V LTV P + E C IR++D AL F + N I L K + Q M +P + + + T+ F A R + C P S ++ + N+V+ S +K ++R++FPE W+F N ++ +LT PDS+TTWRA ++C T+ GLWI + + V +PF+ + +P +K E++ LPV VF C+E+ V V + +G+ ++ C C +++T L P+ +G+LNVT ++ + S ++ + +SD +RRQI V P GVP E TF +++C + + + ++PS ++ GSLR YLT+ +V+G NLD LV +P GCGEQNMV V P++YVL+YL S + + L A+++I+ GY QL Y+ DDGS+SAFG++DK GSTWLTAFVL+ F A + +NI+ L E +L++ Q GCF EKG++ S+M GG+ +K D LLTAYVL SL + +T + + T I +N Y LA SY L +Y P ++ +L+ + + G YWP ++ S D + S ++ D+ETT YA L L ++ ++ + V WLS Q+ S GG+ STQDT++A+ A+A ++ T + N++L V+ + K + L ++V+Q T+ + + + N + C ++Q + YN+ + D T + S C + C R + S M+LV + SGW+ +EE L + +++F + LY++ F+ N+A C + + T + + QPA++ +DYY E Y + + C + E EP P+ LLCP C V+ S+ S L I IC+ + + FV ++ NA + V+ WN ++ NCSC
Sbjct: 25 FITVPSTLYVEHENLISVRTLQQIPSAQLITSIPSLNHKVVDTKQLDL-STKFPDNLYGQDISYKAPFVPITEKSADVQLNFSLTFCTNP---ECTEKTVKHLTADTR---IARRPVIVLGETDKPIYRPGENVRFRFLTLTPQFTVPLNGPINYPTQQLVGGATDG---ELTLDT---FTSEHAETLTRVVYDEIYVKDDKGN-RLKRWLNVSPAEAAKLSYSLLADAQEGKWAVVANVLGETETLSFSVKHFVLPRFVVEISPPKGLTFESNFTEYSVCARYTNGPPMGGSVRSYLCVCDGPGRPETDLSIEDDVLITGTCPGDSGSHAARPCVQQNDILQKDGCVKFSPSTEKLHLKNRDYRIWGQHVIVCAEVKEEGTGSNVTRCEVGEALLRRQATMEITAPSVYKPGLP----ISIALKINGPV-LADAEITVECSESWNSCFRMWHPLSRRPDQGRYQYSTKVKPDSTGFAQLVIPPPSTTSGISVKAIYSLNNTNKRAKRWVVMPPWNEDQSTQQIVAYAHLSAWDSLSGTSMQVWPNEKAFSLSPSGLVTVNLLGNVPLLDKTLIVQTMVRGKVVKHIVRPLDGVTEYCEDRDDNLGHYKCQKNTADGIKCLRGWQGKDCLQPICAAGCNSRGGFCEVPGVCTCRKGWTGSNCEICLKREGCVHGQCVDGNDCVCDKGWQGHLCQLAQVEVESIEEVTEPVSTEETEAVGNLPDTTTEASTVPRRTLYRRNISFPVDGSWGPEAIVIIYFYTEYESTAEIIPAVLELKDLENYTSPAMALQAQTDKAGIQFDRKRVSPGQAVELTVSPAGVDTSALENNLLSGETLSEQVCFIRLSDVALDNFDGNRNL---------------------INLETYAKSVEQLNSANMWRPTVAKTVEEAFQAAGMFHTSVF-AKERIRPDIIAC--------PLMSMMRSGSQMDSDMPAPNAVMANSIPSVKPRLRDFFPEVWLFEAVPIGKINSSSGQPTVGVQQSLTAPDSITTWRASAFCTTKSNGLWIPDSNTVTVSMPFFLELTLPKQVKRGEIIYLPVSVFVARKEELDPKEKGCYEVRVSTRVAREDWLRVGVSDYSGCVCSGEKQTFQLGLVPQRLGQLNVTAEAYAISGSQLCDESQVH---TLSDMIRRQIRVIPEGVPKEATFGDILCLPDGVSHNVLTTPVSVPSSMIPGSLRVYLTYTDEVLGPALSNLDNLVRLPTGCGEQNMVLVAPNVYVLEYLKSGAQVSSKEREKLIHSARTHIMTGYKGQLKYRLDDGSYSAFGQSDKVGSTWLTAFVLRVFSKAYRVDPTLNIDWDRLFNETFTFLKQRQDLEGSGCFVEKGRVIQSSMQGGLGGSKEKNDQALLTAYVLSSLYDLKVTKAATDPKAYESIITNGIRCIKAGVPRDILLPEPLSNVATYGLAQVSYALQMYQPERYDTELYLQELIRRRRTISDALRGPRSYWPANESSDDAESSKWADALDVETTSYAYLTLAERREQAIELLPIVRWLSTQQTSTGGFRSTQDTILAMQAIAHAATGLLYNGTNPGNTNISLSAKVIPSGYKIDDVLNDQKRQVVNQIAIPHTDPNEVQEIMWEVNTDAMSATHCVALQTALFYNVPEPTSTTDDIFTVTATVVQSNPPPGNSCTTAMLTTCLRPASRTDPVQESGMLLVRVQMVSGWKPVQEEILKLVGTDEESVRKVEFDDDGTVSLYFNGFTSNEAEVAGGWSQLKRCVELSLRQTTYVDSTQPALISAMDYYATERSVMIKYQL--EECKPAWLTENEPPVTTTLSATTTEPPLSTPTPKPLLCPKCQVDSSLTSNLSDILYSMICSGYGSMFVMNLYESDENAFNVTMIVVSANGFASTWNATVDRPALSNCSC 1862
BLAST of Alpha-2-macroglobulin vs. TrEMBL
Match: A0A074ZT72 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=T265_13202 PE=4 SV=1) HSP 1 Score: 581.252 bits (1497), Expect = 1.581e-170 Identity = 515/1895 (27.18%), Postives = 822/1895 (43.38%), Query Frame = 1
Query: 106 FITSPNLLITETSHHFNVHT-----STDIVLTIESIH----DPSQIKHQSSKFVIKKGFQTIPYYVPFVG-SHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAI-------------YPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCML--------------------------YSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQ---SW-------------AYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSN---------LKPP------MAATTVYQSLTNFQSKDGHYLQFMPINDKFKCP--GKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYP--SVPVKCYPRDDFFGHYQCVNGTT--LTCLSGWKGGNCREPICSGGC--LNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCS----KKGPIKHLYRNVTT-----VKPITKNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFIN---TDNEVVADALEIASGLNNCS-------QLGGGKFKFNKKTAMPKSKVVLTVDPG----------------VKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYG----LEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSY-----FNKTTKH------LNLTVPDSMTTWRADSYCMTEK-GLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVF--------NYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXIN---IAMSDSVRRQILVEPPGVPTETTFSELICNKN--EQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNS----LNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKY---VNINLSTL-KEATKYLEKMQ--RSDGCFEEKGKLFSSTMAGGIKRN-DKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENIT----------------NNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSF-----GRYKYWPFDKESSDEQKS---NSADIETTGYAILALINHQDLSVS-MKTVYWLSNQRNSLGGYYSTQDTVVALNALA------VFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTE--NISYVSMKTN-----GSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDS----VKQCNIRNIKVCFAYLRRD---KNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKT-RMELYYDAFSYNDAGKDS-------CFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKS--DEKEP-----------------GRPEVLLCPIC-VNDSINSTLKFI--GTICNAWFNGFVT----ANSNASQRRTVFVAEQSMFYEWNLELEP---KNCSC 5055
FIT P+ L E + +V T S ++ +I S++ D Q+ S+KF Q I Y PFV + + ++LN C + E T+ + +TR + + I+ ETDKPIY+PG+ VR RF+ + YPT + G E+ LD F S+ ++ Y ++ + + N R+ +W NVS EA L Y L D G W A G+ E + F +K +VLPRF+VE+ PP + + + VC++Y+ G PM + C+ S+ + CV L K C K ++ + + +R + V E GT S+V++ G + ++ YKPGLP I++ P + + I+++ SW + + + G + VIP +S++ +N + PP Y L+ + S G +Q P F G + + LL N + ++ VQ RG++VK I P V C RDD GHY+C T + CL GW+G +C +PIC+ GC G C P C C++ WTG C C K + C +G CV+G DC C GW C + I+ + V+T V + TE ++T++ R IS P+ + GP+ VI YF + E++ LE+ N S Q +F +K P V LTV P + E C IR++D AL F + N I L K + Q M +P + + + T+ F A R + C P S ++ + N+V+ S +K ++R++FPE W+F N ++ +LT PDS+TTWRA ++C T+ GLWI + + V +PF+ + +P +K E++ LPV VF C+E+ V V + +G+ ++ C C +++T L P+ +G+LNVT ++ + S ++ + + N +SD +RRQI V P GVP E TF +++C + + + ++PS ++ GSLR YLT+ +V+G NLD LV +P GCGEQNMV V P++YVL+YL S + + L A+++I+ GY QL Y+ DDGS+SAFG++DK GSTWLTAFVL+ F A + +NI+ L E +L++ Q GCF EKG++ S+M GG+ + +K D LLTAYVL SL + +T + + T I +N Y LA SY L +Y P ++ +L+ + + G YWP ++ S D + S ++ D+ETT YA L L ++ ++ + V WLS Q+ S GG+ STQDT++A+ A+A ++ T + N++L V+ + K + L ++V+Q T+ + + + N + C ++Q + YN+ + D T + S C + C R + S M+LV + SGW+ +EE L + +++F + LY++ F+ N+A C + + T + + QPA++ +DYY E Y + + C + E EP P+ LLCP C V+ S+ S L I IC+ + + FV ++ NA + V+ WN ++ NCSC
Sbjct: 25 FITVPSTLYVEHENLISVRTLQQIPSAQLITSIPSLNHKVVDTKQLDL-STKFPDNLYGQDISYKAPFVPITEKSADVQLNFSLTFCTNP---ECTEKTVKHLTADTR---IARRPVIVLGETDKPIYRPGENVRFRFLTLTPQFTVPLNGPINYPTQQLVGGATDG---ELTLDT---FTSEHAETLTRVVYDEIYVKDDKGN-RLKRWLNVSPAEAAKLSYSLLADAQEGKWAVVANVLGETETLSFSVKHFVLPRFVVEISPPKGLTFESNFTEYSVCARYTNGPPMGGSVRSYLCVCDGPGRPETDLSIEDDVLITGTCPGDSGSHAARPCVQQNDILQKDGCVKFSPSTEKLHLKNRDYRIWGQHVIVCAEVKEEGTGSNVTRCEVGEALLRRQATMEITAPSVYKPGLP----ISIALKINGPV-LADAEITVECSESWNSCFRMWHPLSRRPDQGRYQYSTKVKPDSTGFAQLVIPPPSTTSGISVKAIYSLNNTNKRAKRWVVMPPWNEDQSTQQIVAYAHLSAWDSLSGTSMQVWPNEKAFSLSPSGLVTVNLLGNVPLLDKTLIVQTMVRGKVVKHIVRPLDGVTEYCEDRDDNLGHYKCQKNTADGIKCLRGWQGKDCLQPICAAGCNSRGGFCEVPGVCTCRKGWTGSNCEICLKREGCVHGQCVDGNDCVCDKGWQGHLCQLAQVEVESIEEVTEPVSTEETEAVGNLPDTTTEASTVPRRTLYRRNISFPVDGSWGPEAIVIIYFYTEYESTAEIIPAVLELKDLENYTSPAMALQAQTNKAGIQFGRKRVSPGQAVELTVSPAGVDTSALENNLLSGETLSEQVCFIRLSDVALDNFDGNRNL---------------------INLETYAKSVEQLNSANMWRPTVAKTVEEAFQAAGMFHTSVF-AKERIRPDIIAC--------PLMSMMRSGSQMDSDMPAPNAVMANSIPSVKPRLRDFFPEVWLFEAVPIGKINSSSGQPTVGVQQSLTAPDSITTWRASAFCTTKSNGLWIPDSNTVTVSMPFFLELTLPKQVKRGEIIYLPVSVFVARKEELDPKEKGCYEVRVSTRVAREDWLRVGVSDYSGCVCSGEKQTFQLGLVPQRLGQLNVTAEAYAISGSQLCDESQDESNKQVHTLSDMIRRQIRVIPEGVPKEATFGDILCLPDGVSHNVLTTPVSVPSSMIPGSLRVYLTYTDEVLGPALSNLDNLVRLPTGCGEQNMVLVAPNVYVLEYLKSGAQVSSKEREKLIHSARTHIMTGYKGQLKYRLDDGSYSAFGQSDKVGSTWLTAFVLRVFSKAYRVDPTLNIDWDRLFNETFTFLKQRQDLEGSGCFVEKGRVIQSSMQGGLGGSKEKNDQALLTAYVLSSLYDLKVTKAATDPKAYESIITNGIRCIKAGVPRDILLPEPLSNVATYGLAQVSYALQMYQPERYDTELYLQELIRRRRTISDALRGPRSYWPANESSDDAESSKWADALDVETTSYAYLTLAERREQAIELLPIVRWLSTQQTSTGGFRSTQDTILAMQAIAHAATGLLYNGTNPGNTNISLSAKVIPSGYKIDDVLNDQKRQVVNQIAIPHTDPNEVQEIMWEVNTDAMSATHCVALQTALFYNVPEPTSTTDDIFTVTATVVQSNPPPGNSCTTAMLTTCLRPASRTDPVQESGMLLVRVQMVSGWKPVQEEILKLVGTDEESVRKVEFDDDGTVSLYFNGFTSNEAEVAGGWSQLKRCVELSLRQTTYVDSTQPALISAMDYYATERSVMIKYQL--EECKPAWLTENEPPVTTTLSATTTEPPLSTPTPKPLLCPKCQVDSSLTSNLSDILYSMICSGYGSMFVMNLYESDENAFNVTMIVVSANGFASTWNATVDRPALSNCSC 1868
BLAST of Alpha-2-macroglobulin vs. TrEMBL
Match: A0A4S2M4B3 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002792 PE=4 SV=1) HSP 1 Score: 575.859 bits (1483), Expect = 2.717e-168 Identity = 518/1899 (27.28%), Postives = 830/1899 (43.71%), Query Frame = 1
Query: 106 FITSPNLLITETSHHFNVHT-----STDIVLTIESIH----DPSQIKHQSSKFVIKKGFQTIPYYVPFV-GSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAI-------------YPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGK--------------------------KKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISI---QSW-------------AYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSN---------LKPPM------AATTVYQSLTNFQSKDGHYLQFMPINDKFKC--PGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYP--SVPVKCYPRDDFFGHYQCVNGTT--LTCLSGWKGGNCREPICSGGC--LNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCS----KKGPIKHLYRNVT-----TVKPITKNETEI---RKFEKQTIFERIISIPLMKNMGPDVHVIAYFIN---TDNEVVADALEIASGLNNCS-------QLGGGKFKFNKKTAMPKSKVVLTVDP-GVKENG---------------CAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGF---TAITNAR----IVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSY-----FNKTTK------HLNLTVPDSMTTWRADSYCMTEK-GLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVF--------NYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINI---AMSDSVRRQILVEPPGVPTETTFSELICNKNE-QLGILSV-FAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNI----KNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDA---SKYVNINLSTL-KEATKYLEKMQ--RSDGCFEEKGKLFSSTMAGGI-KRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENIT----------------NNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSF-----GRYKYWPFDKESSDEQKS---NSADIETTGYAILALINHQDLSVSM-KTVYWLSNQRNSLGGYYSTQDTVVALNALA------VFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTE--NISYVSMKTN-----GSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVK----QCNIRNIKVCFAYLRRD---KNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKT-RMELYYDAFSYNDAGKDS-------CFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEKEPGR-----------------PEVLLCPICVNDS---INSTLKFIGTICNAWFNGFVTANSNASQR---RTVFVAEQSMFYE-WNLELEP---KNCSC 5055
FIT P+ L E + +V T S ++ +I S++ D Q+ S++F Q I Y PFV + + ++LN C + E T+ +TR + + I+ ETDKP+Y+PG+ VR RF+ + YPT + G E+ LD F S+ ++ Y ++ + + N R+ +W NVS EA NL Y L D GIW A G+ E + F +K YVLPRF++E+ PP+ I + + VC++Y+ G PM+ + C+ G+ + CV L K C + L ++ + + +R + V E GT S+V++ G + ++ YKPGLP I++ K P + + I++ +SW + + ++ G + VIP +S++ +N + PP Y L+ ++S G +Q P F G + + L+ N + + ++ VQ RG++V+ I P V C RDD GHY+C N TT + CL GW+G +C +PIC+ GC G C P +C C++ WTG C C K + C +G CV+G DC C GW C ++ I+ + T TV +T + TE+ ++T+++R IS P+ + G + VI YF + + E+V L + N S Q +F++K P V LTV P GV N C IR++D AL F N I L K + Q V M +P + + AG + N R IV M + G + SN+ S + +V +K ++R++FPE W+F N ++ L+L+ PDS+TTWRA ++C T+ GLWI + + V +PF+ + +P +K E++ LPV VF C+E+ V +V + +G+ ++ C C +++T L P+ +G+LNVT ++ + S ++ + N +SD +RRQI V P GVP E TF +++C + G+++ ++P I+ GSLR YLT+ +V+G NLD LV +P GCGEQNMV V P++YVL+YL S + + L A+ +I+ GY QL Y+ +DGS+SAFG++DK GSTWLTAFVL+ F A + + I+ L E +L++ Q GCF EKG++ S+M GG+ +K D LLTAYVL SL + +T+ + + T I +N Y LA SY+L +Y P ++ +L+ + + G YWP ++ S D + S ++ D+ETT YA L L ++ V + V WLS Q+ S GG+ STQDT++A+ A+A ++ T + N++L V+ + K + L ++V+Q T+ ++ + N + C ++Q + YNI + D T S C + C + + S M+LV + SGW+ +EE L + +++F + LY++ F+ +A C + +++ T + + QPA++ +DYY E Y + E EP P+ LCP C DS N + IC+ + N F+ + + T+ V S F WN + NCSC
Sbjct: 96 FITVPSTLYVEHENLISVRTLENIPSAQLITSIPSLNHTIVDTKQLDL-STQFPDNLYGQDISYKTPFVPTAEKSTNVQLNFSLTFCANP---ECTEKTVKYLTADTR---IARRPVIVLGETDKPVYRPGENVRFRFLTLTPQFTVPLNGPVNYPTQQLVGSATDG---ELALDT---FTSEHAEKLIRVVYDEIYVKDDKGN-RLKRWLNVSPAEAANLSYSLLADAREGIWTIVAHVLGETETLSFSVKHYVLPRFVMEISPPNNITFESNFTEYSVCARYTNGPPMSGSVRSYLCVCDKPGRSETDLPTEGDVLITGTCPGGSGSQPPRPCVQQNDILQKDGCVRFGLSTEKLHLKNRDYRIWGQHVIVCAEVEEEGTGSNVTRCEVGDELLRREVTMEITAPSVYKPGLP----ISIALKIKGP-NLADAEITVECSESWNSCFRMWRPLPREPEQGRYQYSTKVKPDRTGFAQLVIPPPSTTNGISVKAIYSLNNTNKRVKRWVIMPPWNDDQSNQQIVAYAHLSAWESLSGTSMQTWPNEKAFSLSPSGLVTVNLMANVPLLNKTVIVQTMVRGKVVQHIIRPLNGVTEYCEDRDDSLGHYRCQNNTTDGIECLHGWQGKDCLQPICAPGCSLRGGFCEMPWQCKCRKGWTGSNCEICLKQEGCVHGQCVDGNDCVCDEGWQGHLCQLARVQQERIEEVMEPDTTEKTETVDSLTDSTTEVLWPSTVPRRTLYQRNISFPVDGSWGSEATVIIYFYSKYESTAEIVPAVLHLKDLENYTSPAMALQTQTNTTGIRFDRKRVSPGQAVKLTVSPAGVDTNALQNSLLSSDTLSEQVCFIRLSDVALDNFDGEKNL---------------------INLETYAKSVEQLSVANMWRPTVAKTVE--EAFQAAGMYHTSFFANERIRPDIVACPLMSMMRSG-------SQSNQMEVDSPAARAVTANLMPGVKPRLRDFFPEVWLFEAAPIGTINSSSGQPTVGVQLSLSAPDSITTWRASTFCTTKSNGLWIPDSNTVAVSMPFFLEMTLPKQVKRGEIIYLPVSVFVVREEELDPKEKGCYEVRVSTSVAREDWLPVGVNDYSGCVCSGEKQTFQLGLVPQRLGQLNVTAEAYAISGSQLCDESQDVSNRQVHTLSDMIRRQIRVIPEGVPKEATFGDILCLPDGVSHGVVTTPVSVPPSIIPGSLRVYLTYTDEVLGPALSNLDNLVRLPTGCGEQNMVLVAPNVYVLEYLKSGALVSSKEREKLMHSARMHIMTGYKGQLKYRLEDGSYSAFGQSDKVGSTWLTAFVLRVFTKAYRVDRTLKIDWDRLFNETFTFLKQRQDLEGSGCFVEKGRVIQSSMQGGLGGSKEKNDQALLTAYVLSSLYDLRVTSAARDYDAYESVITNGIRCIKAGVPRDILLPEPLSNVATYGLAQVSYVLQMYQPERYDTELYLQELMRRRHTISDALRGPRSYWPANEPSEDTESSKWADALDVETTSYAYLTLAERREQVVELFSIVRWLSTQQTSTGGFRSTQDTILAVQAIAHAATRLLYNGTNPGNTNISLSAKVIPSGYKIDDVLSDQKRQVVNQIAIPHTDPKDVQEIMWDVNTDAMSATHCVALQTALFYNIPEPTSTTDGIFTMTATVAQSRPPPGNSCTTATVTTCLRPASKTDPVQESGMLLVRVQMVSGWKPVQEEILKLVGSEEESVRKVEFDDDGTVSLYFNGFTSGEAKVAGGWSQLKRCVQLNLQQTTYVDSTQPALISAMDYYATERSVMIKYQLEECKSAWLTENEPSVITNVSTATTETPLSTSIPKPRLCPKCQLDSGLKSNLSDSLYAMICSGYNNMFMMDLYESDEEVFNVTMIVVSASGFASTWNATVNRAALSNCSC 1945
BLAST of Alpha-2-macroglobulin vs. Ensembl Cavefish
Match: ENSAMXT00000031363.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:15733406:15761969:1 gene:ENSAMXG00000010565.2 transcript:ENSAMXT00000031363.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 335.88 bits (860), Expect = 1.077e-93 Identity = 233/735 (31.70%), Postives = 387/735 (52.65%), Query Frame = 1
Query: 2650 IREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKS----FTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSV--KTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPL-NRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+R++FPE+W++ + + + +TVPD++TTW D++C++ +G+ +AP +L+V PF+ + +PYS+ E +L VFNY C I VK+ + + S ++ C C N KT + L P ++G LNVT+ ++++ S + + + D+V R +LV+ G + S L+C + + +P +++ GS R+ ++ GD++G+ N+D L+ MPYGCGEQN+ + P+IY+L YL S PA+ KA ++ GY RQL YKH G +S FG ++ +TWLTAFV+++F A +Y+ I+ + A K+L Q+S+GCF +G LF++ M GG+ +D +TAY+ SL ++L T + K ++C + + Y AL +Y +L + Q + QL ++S +W S + S+S +E + Y +LA+ ++ DL + + V WL Q+N GG+ STQDTVVAL ALA++A + S + + V+V +T++ + +N+L+ Q +L Y S+ NGS C S+Q++ YNI DT LSV KTE D K +I Y+ ++NM++V I SG+ + S PL R+D + + +Y + N K ++ I++ + NL+PAV+K+ DYY P SE YS
Sbjct: 685 VRKFFPETWIWQLVKVGDSGSAQVPVTVPDTITTWEMDTFCLSSRGVGVAPAAQLRVFQPFFLELSLPYSIIRGEEFELKATVFNYLSKC--IMVKVTPAPSSDYTLKASSSDEQYSSCLCANGRKTFRWTLVPSVLGVLNVTVSAAAEPSQTVCNNEIVSVPDRGRIDTVTRSLLVQAEGTEQTKSQSWLLCPQGNSVS--EELTLPEDVIEGSARASVSVLGDILGRALKNIDGLLKMPYGCGEQNIALLSPNIYILQYLESTEQLTPAIREKATGFLKSGYQRQLNYKHITGGYSTFGTGEE--NTWLTAFVMRTFGKAQRYIFIDPENINSAKKWLISKQKSNGCFVRQGSLFNNRMKGGV-----SDDVTMTAYITASL--LELGQTDPVVRKGLSCLKLFVNSRKTTYTTALLAYTFTLAGENEIRGQ-LLKQLDRSAISGDGRLHW----SQSASEDSDSLSVEISSYVLLAVLSTDKLSAADLGYANRIVSWLVRQQNPYGGFSSTQDTVVALQALALYATKV-YSPDGSSTVTVQSANKETHSFEVNQNNKLLYQEKSLQNVPGEY-SVIVNGSNCVSVQMALFYNIP---TPTDTSTLSVKAKTEGDCKKNPQTLSINFTVKYIGLLDSTNMVIVDIKILSGFTADPSTSGTRS---GMPLVERVDSKDDHVIMYINKILKN---KPLNYQLHIKQLLPVKNLKPAVIKVYDYYQPNDQSETEYS 1390 HSP 2 Score: 85.8853 bits (211), Expect = 4.062e-16 Identity = 88/337 (26.11%), Postives = 141/337 (41.84%), Query Frame = 1
Query: 382 FINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEA-MNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLY-SYGKKKCVAFKSDLMKQNCNKIKLESKIV--FILASSFRYSKAKSQI------NVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKPAKMVNISI-SIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
+ ETR++ N + F +TDKPIY PG L F+ P + E D++ DN+ N R+ QW NVS + + L +QLN + G + KA+ + F +KKYVLPRF + V P E +E + VC KY+ G+P++ K + C + S + F D C + K + V S+F ++ + + VTVTE GT S+ K+ E + Y ++ P Y++ G G+I ++ + KP ++ + Q W W T++ G+ F I
Sbjct: 110 LMAETRKVMFKSYNPLTFIQTDKPIYNPGQTGNLTFLHFVPK--ITEHDKV--------------------TDNNGN-------------RIGQWTNVSSTDLILELSHQLNSEAPEGQYTLKAITGDRSITHHFTVKKYVLPRFEITVNAPKEHSVGEEELKIDVCGKYTYGQPVSGKALILVCRPFKSRSPIYSIPFI-DPQTLLC-RTKTDGGCVSATFTVSTFTNTELEHHLKDFLDAKVTVTEEGTDISMVKS-ETISLTYQIGKVELLDLPKYFEYGTYIDGKIKVLTFSGKPVPHRSVYLFKHQMW----QWNEHLTLTTDINGLANFTI 404
BLAST of Alpha-2-macroglobulin vs. Ensembl Cavefish
Match: ENSAMXT00000010848.2 (pep primary_assembly:Astyanax_mexicanus-2.0:1:15733307:15761838:1 gene:ENSAMXG00000010565.2 transcript:ENSAMXT00000010848.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 332.798 bits (852), Expect = 9.477e-93 Identity = 229/726 (31.54%), Postives = 382/726 (52.62%), Query Frame = 1
Query: 2650 IREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKS----FTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSV--KTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPL-NRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNP 4779
+R++FPE+W++ + + + +TVPD++TTW D++C++ +G+ +AP +L+V PF+ + +PYS+ E +L VFNY C I VK+ + + S ++ C C N KT + L P ++G LNVT+ ++++ S + + + D+V R +LV+ G + S L+C + + +P +++ GS R+ ++ GD++G+ N+D L+ MPYGCGEQN+ + P+IY+L YL S PA+ KA ++ GY RQL YKH G +S FG ++ +TWLTAFV+++F A +Y+ I+ + A K+L Q+S+GCF +G LF++ M GG+ +D +TAY+ SL ++L T + K ++C + + Y AL +Y +L + Q + QL ++S +W S + S+S +E + Y +LA+ ++ DL + + V WL Q+N GG+ STQDTVVAL ALA++A + S + + V+V +T++ + +N+L+ Q +L Y S+ NGS C S+Q++ YNI DT LSV KTE D K +I Y+ ++NM++V I SG+ S PL R+D + + +Y + N K ++ I++ + NL+PAV+K+ DYY P
Sbjct: 673 VRKFFPETWIWQLVKVGDSGSAQVPVTVPDTITTWEMDTFCLSSRGVGVAPAAQLRVFQPFFLELSLPYSIIRGEEFELKATVFNYLSKC--IMVKVTPAPSSDYTLKASSSDEQYSSCLCANGRKTFRWTLVPSVLGVLNVTVSAAAEPSQTVCNNEIVSVPDRGRIDTVTRSLLVQAEGTEQTKSQSWLLCPQGNSVS--EELTLPEDVIEGSARASVSVLGDILGRALKNIDGLLKMPYGCGEQNIALLSPNIYILQYLESTEQLTPAIREKATGFLKSGYQRQLNYKHITGGYSTFGTGEE--NTWLTAFVMRTFGKAQRYIFIDPENINSAKKWLISKQKSNGCFVRQGSLFNNRMKGGV-----SDDVTMTAYITASL--LELGQTDPVVRKGLSCLKLFVNSRKTTYTTALLAYTFTLAGENEIRGQ-LLKQLDRSAISGDGRLHW----SQSASEDSDSLSVEISSYVLLAVLSTDKLSAADLGYANRIVSWLVRQQNPYGGFSSTQDTVVALQALALYATKV-YSPDGSSTVTVQSANKETHSFEVNQNNKLLYQEKSLQNVPGEY-SVIVNGSNCVSVQMALFYNIP---TPTDTSTLSVKAKTEGDCKKNPQTLSINFTVKYIGLLDSTNMVIVDIKILSGFTADPSTVS------SMPLVERVDSKDDHVIMYINKILKN---KPLNYQLHIKQLLPVKNLKPAVIKVYDYYQP 1366 HSP 2 Score: 84.3445 bits (207), Expect = 1.306e-15 Identity = 90/346 (26.01%), Postives = 139/346 (40.17%), Query Frame = 1
Query: 382 FINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKN------DNDSNYFDLIIIETPDNVRVAQWKNVSINEA-MNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLY-SYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYS-----------KAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKPAKMVNISI-SIQSWAYGNNWGFIRNYTSNKKGVIEFVI 1356
+ ETR++ N + F +TDKPIY PG V R V+ ++K I K DN+ N R+ QW NVS + + L +QLN + G + KA+ + F +KKYVLPRF + V P E +E + VC KY+ G+P++ K + C + S + F D C LE ++++F S K VTVTE GT S+ K+ E + Y ++ P Y++ G G+I ++ + KP ++ + Q W W T++ G+ F I
Sbjct: 84 LMAETRKVMFKSYNPLTFIQTDKPIYNPGQTVNFR----------------------VVTMDSKLIPLEQKVRGIFNCDNNGN-------------RIGQWTNVSSTDLILELSHQLNSEAPEGQYTLKAITGDRSITHHFTVKKYVLPRFEITVNAPKEHSVGEEELKIDVCGKYTYGQPVSGKALILVCRPFKSRSPIYSIPF-IDPQTLLCRTKTLEMSDGGCVSATFTVSTFTNTELEHHLKDFLDAKVTVTEEGTDISMVKS-ETISLTYQIGKVELLDLPKYFEYGTYIDGKIKVLTFSGKPVPHRSVYLFKHQMW----QWNEHLTLTTDINGLANFTI 388
BLAST of Alpha-2-macroglobulin vs. Ensembl Cavefish
Match: ENSAMXT00000005486.2 (pep primary_assembly:Astyanax_mexicanus-2.0:6:30175654:30201397:1 gene:ENSAMXG00000005291.2 transcript:ENSAMXT00000005486.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 318.546 bits (815), Expect = 3.577e-88 Identity = 259/930 (27.85%), Postives = 432/930 (46.45%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMP--KSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLS-TAGFTAITNARIVN-VKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKS--FTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLD-QSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQ-----DLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQIST-PLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYN 4776
I + +KNM P V+ Y I + E VAD+ + A L +Q+ F T +P ++ ++L PG + C+++ D +LL D+ Y L + I + + CI + ++ PI G S G +TNA + V C + PD + + + IR YFPE+W++ + TVPD++TTW A ++CM+ G IAP +L PF+ ++ +PYS+ EVL L VFNY C + V + + + + +T C C ++ T + ++ +GKL V + + + C + D+V + +LV+ G T++EL+C ++L S +L GD+MG+ NL L+ MPYGCGEQNM+ P+I++L YL S P + +KA ++++ GY R+LTYKHDDGS+SAFG +D G+TWLTAFV+KSF A KY+ ++ + +A +L Q+ +GCF G+LF S M GG+ +D LTAY+ ++ ++L + K++ C N T Y +AL SY +L +++ +LD Q++ ++W +D + +S ++E T Y +LA+++ DL V WL+ Q+N+ GG+ STQDTVVAL ALA ++ + + N + NRL+ Q +L + +Y S++ G+GC +Q + YNI A +F + + V Y +NM+++ + SG+ L E+ + + + R+D ++ + +Y D + G+ + I ++T + L+PAV+K+ DYY
Sbjct: 493 IQLEHVKNMAPFAQVVVYTILPNGEAVADSRDFAVDLCLTNQV---SLIFPSPTELPGGQTSLILKAQPG---SLCSVKAIDRSLLLLKPEKELDIDYCDHTLSGYPYNIYDE------EPNPCISRPPIR---PPIIGYTVTISACSSQNIGIKILTNAEVRKPVDCSGPIFLAPPDVA-----------------------VQSDQTIRTYFPETWIWDLVPVGKSGIMGVKKTVPDTITTWSARAFCMSAVGFGIAPKAELTTFQPFFVSLTLPYSIIRGEVLPLKATVFNYLSGCIMVKVTL-AHSEQFTAQQCERCIYTRCLCADESWTFKWTVQASALGKLCVA--EALQTNVLCGSRSVTVPEKGRMDTVVQTLLVKAEGTKQTNTYNELLCMTGQKL-------------SPHFELHLLSTGDLMGRALKNLQSLLAMPYGCGEQNMLLFAPNIFILLYLESSGQLTPEIRSKATTFLVSGYQRELTYKHDDGSYSAFGMSDPSGNTWLTAFVMKSFSSAMKYIFVDQQYVDKAKDWLGLQQQENGCFASVGQLFHSDMKGGV-----SDELTLTAYITAAM--LELGTPDPVVEKSLTCLKSNFTQLNSTYSIALLSYAFTLAEDHQMRLESL--NILDEQAIVTAGGRHW---SRLNDGKTLDSLEVEMTSYVLLAILSGPQLQGFDLGYCASIVRWLAQQQNAFGGFASTQDTVVALQALAKYSAVTYSPAGTTVVTVTGPSGNTLTTFTVNQSNRLLVQESSLQQTSGNY-SIRAEGNGCVLVQFTLNYNIPPP-ANYLSFNIFANISAKCSDLIPSAELSVTVRYNGNRVETNMVIIEVRLLSGFRLDEKSLFLSNDTSAVGAIKRVDQKQDTVFIYLDGLT---RGEFKTYHLVILQQTVVQKLKPAVIKVYDYYQ 1351 HSP 2 Score: 95.1301 bits (235), Expect = 6.087e-19 Identity = 100/441 (22.68%), Postives = 179/441 (40.59%), Query Frame = 1
Query: 382 FINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVS-INEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC------MLYSYGKKK----CVAFKSDLMKQNCNKIKLESKIVFILASSFR----YSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPY-YKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVI-PYIPLAQSLSIQISEVSS-----NLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKC--PGKLNLTLLTNYDI-----ASTSIYVQIFSRGQIVK 1614
F+N+T +I + + +TDKPIYKPG V++R ++ ++ F + P++ R+ QW N S + ++L Y LN + G + + E+I Q FEI +YVLP F V V P I D + KVC Y+ GKP+N + + C + Y K C + K C L + + A+ + + V NGT S+ I N NI F+ P ++ G+P+ G+I + N PAK ++ + ++ Y ++ ++ G F + + ++S+S+++SE +++ P + Y + F S+ +L+ P F C P +N + N ++ S + + + S+G +V+
Sbjct: 84 FLNKTTKILITPPVRLTIIQTDKPIYKPGQTVKIRIASL------------------------------------TSSFQTYDQQDPNSNRIGQWLNQSTFSGILDLSYPLNSEAQHGFYIITVWNERNEQITQDFEINEYVLPTFEVIVQLPPVITILDTEATLKVCGNYTYGKPVNGSVIAVVCRKHYVWWWFGYDSKSNLDICKTYSMTTDKAGCGSAVLNLEEYALTATEYEDVINVEAQLEEAGTGVVLNGTGSTT--------ITINMLNINFEETPASFRLGMPFNGKIKVTGPNSVPAKDEHVYLIVR---YDDDKNVTWTLVTDANGTAPFSLNTALWGSKSVSLEVSESVEQYGYMSVRKP-TYFSAYLWVQPFYSESNCFLELQPSPRPFYCNKPAVVNARYIINSNVLTQCQKSMTFFYMVMSKGSMVQ 476
BLAST of Alpha-2-macroglobulin vs. Ensembl Cavefish
Match: ENSAMXT00000010844.2 (pep primary_assembly:Astyanax_mexicanus-2.0:1:15710065:15729202:1 gene:ENSAMXG00000010515.2 transcript:ENSAMXT00000010844.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 307.375 bits (786), Expect = 1.556e-84 Identity = 221/752 (29.39%), Postives = 380/752 (50.53%), Query Frame = 1
Query: 2635 KMKKQIREYFPESWVFS---YFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKS----FTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQ-----SVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQC-----NIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYN-----DAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
+ ++ E F ++F+ + + + + +TVPD++TTW D++C++ +G+ +AP +L+V PF+ + +PYS+ E +L VFNY C I VK+ + + S ++ C C N KT + L P ++G LNVT+ ++++ S + + + D+V R +LV+ G + S L+C + + +P +++ GS R+ ++ GD++G+ N+D L+ MPYGCGEQN+ + P+IY+L YL S PA+ KA ++ GY RQL YKH +G++S FG G+TWLTAFV+++F A +Y+ I+ + A K+L + Q DGC+ +G+LF++ M GG+ D +TAY+ SL ++L +T ++K ++C +I + + + L YT S + + QLL + V GR +W S + S+S +E + Y +LA+ +++ DL + + V WL Q+N GG+ STQDTVVAL ALA+++ + S N + V+V +T++ + +N+L+ Q +L Y S+ GS CAS+Q+S YNI N+ L ++ + D C ++ ++ Y + ++NM++V I SG+ N + R + ++ I++ + NL+PAV+K+ DYY P SE YS
Sbjct: 686 RFHRRYGERFDRLYMFALLFHSDSGSAQVPVTVPDTITTWEMDTFCLSSRGVGVAPAAQLRVFQPFFLELSLPYSIIRGEEFELKATVFNYLSKC--IMVKVTPAPSSDYTLKASSSDEQYSSCLCANGRKTFRWTLVPSVLGVLNVTVSAAAEASQTVCDNEIVSVPERGRIDTVTRSLLVQAEGTEKSKSQSWLLCPQGNSVSEELELTLPGDVIEGSARASVSVLGDILGRALQNIDGLLKMPYGCGEQNIALLSPNIYILQYLESTEQLTPAIREKATGFLKSGYQRQLNYKHYNGAYSTFGTGQ--GNTWLTAFVMRTFGKAQRYIFIDPENINSAKKWLLENQTPDGCYIRQGRLFNNRMKGGVN-----DNVTMTAYITASL--LELGHTDPVVSKGLSCIKSSIGDL--KHLKHTYASALLAYTFSLAGENEIRGQLLKKLDTVAIVGDGRL-HW----SRSATEDSDSLSVEISSYVLLAVLSTDKLSNADLGYANRIVSWLVRQQNPYGGFSSTQDTVVALQALALYSTKV-YSSNGSSTVTVQSADKETHSFEVNQNNKLLYQEKSLQNVPGKY-SVSVKGSTCASVQMSLFYNIP---TPNEVRTLGIEAKADG--NCTKSFGHVLSVNFTVKYNGKLNSTNMVIVDIKILSGFTGDP--------------NEVSISGKRFYCMFTVLCLMCVHQVPTNSPVHYQLHIKQVLPVKNLKPAVIKVYDYYQPSDQSETEYS 1398 HSP 2 Score: 79.337 bits (194), Expect = 4.042e-14 Identity = 89/340 (26.18%), Postives = 139/340 (40.88%), Query Frame = 1
Query: 382 FINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVS-INEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKS-----DLMKQNCNKIKLESKIVFI---LASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIK----FDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWG--FIRNYTSNKKGVIEFVI 1356
+ ETR++ + F +TDKPIY PG KV R + + N + E+ K V D N KN R+ QW NVS + L +QLN + G + KA+ + F +KKYVLP+F V + P E +E + +VC KY+ G+P+ C + V S L +N + + VFI A +FR S I+VT+TE GT S+ ++ G +F + D +++ G+I + H+ KP + + G +W + N T++ G+ F I
Sbjct: 110 LMTETRKVVFKSYKPLTFIQTDKPIYNPGQKVNFRIVTM-NANFVPLRQEVSK----VFDSN--------KN------------------RIGQWTNVSSTGLILQLSHQLNSEASEGQYTLKAIIEDTWITHHFTVKKYVLPKFEVTLKAPKEHSVGEEELKIEVCGKYTFGQPVPGTALVQVCRKSRQRYFQGVQVVSPCLYETLTVENGCASHIYNMSVFIGSEFAHNFRESL---DIDVTMTEEGTGISMVQS----GRTEFTFVLGKAEFLDLPKHFERDTVIQGKIKVTTHSGKPIPNKEVYL-----LNGYSWQSKLLLNLTTDDNGLASFTI 406
BLAST of Alpha-2-macroglobulin vs. Ensembl Cavefish
Match: ENSAMXT00000010840.2 (alpha-2-macroglobulin-like [Source:NCBI gene;Acc:103040601]) HSP 1 Score: 305.449 bits (781), Expect = 7.767e-84 Identity = 216/705 (30.64%), Postives = 361/705 (51.21%), Query Frame = 1
Query: 2383 LRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYF---NKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKS----FTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILAL-----INHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDS 4458
+R+ +C+ K V+ + YG YDSR ++ G + A V M Y + D SY T +R++FPE+W++ + + +TVPD++TTW D++C++ +G+ +AP +L+V PF+ + +PYS+ E +L VFNY C I VK+ + + S ++ C C N KT + L P ++G LNVT+ ++++ S + + + + D+V R +LV+ G + S L+C + + +P +++GS R+ ++ GD++G+ N+D L+ MPYGCGEQN+ + P+IY+L YL + PA+ KA ++ GY RQL YKH +G++S FG+ + G+TWLTAFVL++F A +Y+ I+ + + ++L Q DGCF +G+LF++ M GG+ D +TAY+ SL + + K ++C ++ T Y AL +Y SL + Q + +L ++S +W S + S+S +E + Y +LA+ +++ DL + V WL Q+N GG+ STQDTVVAL ALA+++ + S + V+V +T++ + +N+L+ Q +L Y S+ NGS CAS+QL+ YNI + LSVK E D+
Sbjct: 676 VREPQCLVYKGVEYH-RGGYGWRYDSRVGMAGPGAPPVALAMGV---AMSYESEDS-DNSYET--------------------------VRKFFPETWIWQLAEVGGSGSAQVPVTVPDTITTWEMDTFCLSSRGVGVAPAAQLRVFQPFFLELSLPYSIIRGEEFELKATVFNYLSKC--IMVKVTPAPSSDYTLKASSSDEQYSSCLCANGRKTFRWTLVPSVLGVLNVTVSAAAEASQTVCDNEIVSVPDRGRIDTVTRSLLVKAEGTENSKSQSWLLCPQGNSVSEELELTLPENVIAGSARASVSVLGDILGRALKNIDGLLKMPYGCGEQNIALLSPNIYILQYLENTQQLTPAIREKATGFLKSGYQRQLNYKHFNGAYSTFGRGE--GNTWLTAFVLRTFGKAQRYIFIDPENIDQTKQWLISKQGPDGCFIRQGRLFNNRMKGGVN-----DNVTITAYITASLLELGHKAEDPLVIKGLSCLKSSLGTITNTYATALLAYTFSLAGENEIRGQ-LLKKLDGDAISGDGRLHW----SQSASEDSDSLSVEISSYVLLAVLSTDKLSNADLGYGNRIVSWLVRQQNPYGGFSSTQDTVVALQALALYSTKV-YSPDGFSTVTVQSADKETHSFEVNQNNKLLYQEKSLQNVPGKY-SVSVNGSTCASVQLALFYNIPTPIVPS---TLSVKVEADA 1330 HSP 2 Score: 80.4925 bits (197), Expect = 1.858e-14 Identity = 82/334 (24.55%), Postives = 144/334 (43.11%), Query Frame = 1
Query: 385 INETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVS-INEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLE-SKIVFILA----SSFRYSKAKS-QINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPY-YKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWG--FIRNYTSNKKGVIEFVI 1356
+ E R++ N + F +TDKPIY PG V R + + G + + ++ N + DN N R+ QW NVS + L +QLN + G +K +A+ + + F +KKYVLPR+ + V P E +E + +VC KY+ G+P+ K C + + V + + +N + + VF ++ S F ++ S INV +TE GT S+ K+ E + Y +F P ++ G+I +V + +P + + + G +W + N T++ G+ +F +
Sbjct: 112 MTEKRKVMFKSYNPLTFIQTDKPIYNPGQTVHFRVVTM------------GADLVPLEEEYNIL----TLADNKGN-------------RIGQWTNVSSTGLILQLSHQLNSEASEGQYKLEAITEDRSITHFFTVKKYVLPRYEITVKVPKEQSVGEEDLKIEVCGKYTYGQPVPGKARVEVCRKFERYYYEGVQMITPCLHENVEMDEDGCATHVFNMSLFSNSEFEHNFRDSLDINVVMTEEGTGISMVKS-ERTSVTYEIGKAEFLDLPKNFERDTVIEGKIKVVTYGGEPIRNKEVYL-----LKGPSWSPTLLLNLTTDDDGLAKFAV 410
BLAST of Alpha-2-macroglobulin vs. Ensembl Sea Lamprey
Match: cpamd8 (C3 and PZP like alpha-2-macroglobulin domain containing 8 [Source:ZFIN;Acc:ZDB-GENE-050208-428]) HSP 1 Score: 284.648 bits (727), Expect = 8.666e-78 Identity = 227/769 (29.52%), Postives = 377/769 (49.02%), Query Frame = 1
Query: 2632 AKMKKQIREYFPESWVFSYFNKTT---KHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANE--INVIGLKSFT--LCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXX--XXXXXINIAMSDSVRRQILVEPPGVPTETTFSELIC-------NKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKL-LTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKES----------SD--EQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVV---LNRNKTYNGLITLDNRLVSQTFNLGTENISYVS---MKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVK-TEIDSVKQCNIRN--------------IKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNP 4779
+ K+ R +FPE+W++ FN L + VPDS+T+W A++ ++ +GL +AP + L+ PF+ + +PY++ E ++P+ V NY C E++VK+ V + G + T +C ++ KT L +G+ N+T+ + S ++ + + +D+ RQ+ ++ G+P E T+S C N NE A+P +V GS R+ + GDVMG T +NLD L+ +P+GCGEQNM+ P+++VL YL + +A Y++ GY RQLTYK DGS+SAFG+ D GS WLTAFVLKSF + ++ I+ L A ++ Q+ DG F G++ + + GGI GK+ LTAYVL +L G D + +A N+ + Y AL++Y L+L S + +L + +++ K+W + SD Q SA++E T YA+L D++ ++ V WLS QRN+ GG+ STQDT VAL +L+ +A + VNL +S+ L+ +++ + DN+ + QT + ++N+ + + + G GC +Q+ YN+ AA F+L+V+ TE S + +N + C + +SNM ++ + SG+ E + + L L R + ++ Y+D +C +F + + + P VKI DYY P
Sbjct: 752 GRADKRKRTFFPETWIWHCFNTRATGEARLQVEVPDSITSWVAEALAISPAQGLGLAPARSLRTFKPFFVDFTLPYALIRGEQAKVPLTVHNYMPLCAEVHVKVTVPKGVKFVGHHGKQHLTRKMCVEADETKTTSLVLSFAELGQTNITVMPVCSPACSASVCVRRGELLRGGGADAQGRQLRMQAEGMPREYTYSVFFCPNENTKGNHNEAF----TLAVPHSVVPGSERATASMIGDVMGPTLNNLDNLLKLPFGCGEQNMIHFAPNVFVLKYLQQTKQLVADVRTEATDYLLQGYQRQLTYKRQDGSYSAFGERDSSGSMWLTAFVLKSFAQSRAFIYIDPKELAAAKAWIINHQKEDGSFPAVGRILNKDIQGGIH------GKISLTAYVLAALMETGTDNEGEKTAVARAKMFLESNMYSAEDPYTTALSAYALTLLHSSYAPV--ALRRLNNVAITQDGLKHWSLSGTTVTNDDTFMGFSDGLSQSVVSAEVEMTAYALLTYTALGDVATALPIVKWLSQQRNAQGGFSSTQDTCVALQSLSEYA-ILSYIGGVNLTISLASTNLDFQESFE--LNKDNKKILQTATV-SQNVLFQTKSPICAKGEGCCLMQIDVSYNVPDP-AAKPAFQLTVELTEPRSTRAQIKKNKHGRHSCVHRCTCCVFTCACRWLQTGSSNMAVLEVPLLSGFRADVESLEQVLLDKKLGLKRYELDGRKVLFYFDEIPNQCM---TCVKFHADREYIVGQTAPVPVKIYDYYEP 1500 HSP 2 Score: 79.7221 bits (195), Expect = 5.783e-15 Identity = 91/353 (25.78%), Postives = 145/353 (41.08%), Query Frame = 1
Query: 379 NFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVS--INEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKIS-GMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSF-----------NIKF--DGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTS---NKKGVIEFVIPYIP-LAQ 1377
+F N T I + K + +TDKP+YKP +V F+ I P E I ++VE I+I+ P R+ QW ++ MN+ + +++ PIFG W FE++KYVLP+F V++ PS I + +V ++Y+ GKP+ K + M Y ++ DL +++ F + K NV G S + V G K +F +IK+ + R +KPGLPY G++ + + PA V + I + A N YTS ++ G++ F I IP +AQ
Sbjct: 111 SFQNST-TITVEQKGSTVLIQTDKPVYKPKQRVLANFIIISP-----ELRPINEKVEA-----------------------YIVIQDPRGSRMVQWSDLHPICCGIMNVSFPISEQPIFGEWMIFVELHSHTYNKSFEVQKYVLPKFEVDISTPSFIRNLNVCDKARVRARYTFGKPVRGKATVNMTVNGVGY-------YRQDLGMPVLKTFEVDGAASFDIC-----VKDLMPPNVPEHFRGALSVWATVVASDGSKQITFDDSTLVHKQLVDIKYSRETRKQFKPGLPYHGKLEVTYPDGSPADGVTVRIKAELAAKDN------IYTSELVSRDGIVAFQIHSIPTMAQ 416
BLAST of Alpha-2-macroglobulin vs. Ensembl Sea Lamprey
Match: cpamd8 (C3 and PZP like alpha-2-macroglobulin domain containing 8 [Source:ZFIN;Acc:ZDB-GENE-050208-428]) HSP 1 Score: 280.796 bits (717), Expect = 9.462e-77 Identity = 225/767 (29.34%), Postives = 368/767 (47.98%), Query Frame = 1
Query: 2632 AKMKKQIREYFPESWVFSYFNKTT---KHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFE-----------INVKINVDANE--INVIGLKSFT--LCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILS---VFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKL-LTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFA-----QLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVV---LNRNKTYNGLITLDNRLVSQTFNLGTENIS-YVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVK-TEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACN 4827
+ K+ R +FPE+W++ FN L + VPDS+T+W A++ ++ +GL +AP + L+ PF+ + +PY++ E ++P+ V NY C E ++VK+ V + G + T +C ++ KT L +G+ N+T+ ++ I + +S ++ G+P E T+S C E G + A+P +V GS R+ + GDVMG T +NLD L+ +P+GCGEQNM+ P+++VL YL + +A Y++ GY RQLTYK DGS+SAFG+ D GS WLTAFVLKSF + ++ I+ L A ++ Q+ DG F G++ + + GGI GK+ LTAYVL +L G D E + + N A Y +L + +L + +A +L + +++ K+W S + SA++E T YA+L D++ ++ V WLS QRN+ GG+ STQDT VAL +L+ +A + VNL +S+ L+ +++ + DN+ + QT + + +VS K G GC +Q+ YN+ AA F+L+V+ TE V +C + C + +SNM ++ + SG+ E + + L L R + ++ Y+D +C +F + + + P VKI DYY P + Y + N C
Sbjct: 694 GRADKRKRTFFPETWIWHCFNTRATGEARLQVEVPDSITSWVAEALAISPAQGLGLAPARSLRTFKPFFVDFTLPYALIRGEQAKVPLTVHNYMPLCAETAFVVGLSIMQVHVKVTVPKGVKFVGHHGKQHLTRKMCVEADETKTTSLVLSFAELGQTNITV---CASRRTQRPQRMTVITVKLS--------MQAEGMPREYTYSVFFCPNEESDGNHNEAFTLAVPHSVVPGSERATASMIGDVMGPTLNNLDNLLKLPFGCGEQNMIHFAPNVFVLKYLQQTKQLVADVRTEATDYLLQGYQRQLTYKRQDGSYSAFGERDSSGSMWLTAFVLKSFAQSRAFIYIDPKELAAAKAWIINHQKEDGSFPAVGRILNKDIQGGIH------GKISLTAYVLAALMETGTD---------------NEVMVETSSNMYSAEDPYTTALSAYALTLLHSSYAPVALRRLNNVAITQDGLKHWSL---SGRARLVVSAEVEMTAYALLTYTALGDVATALPIVKWLSQQRNAQGGFSSTQDTCVALQSLSEYA-ILSYIGGVNLTISLASTNLDFQESFE--LNKDNKKILQTATIPSIPTGLFVSAK--GEGCCLMQIDVSYNVPDP-AAKPAFQLTVELTEHSCVHRCTC-CVFTCACRWLQTGSSNMAVLEVPLLSGFRADVESLEQVLLDKKLGLKRYELDGRKVLFYFDEIPNQCM---TCVKFHADREYIVGQTAPVPVKIYDYYEPGSSRDHQYKL-NITCG 1414 HSP 2 Score: 75.8702 bits (185), Expect = 9.652e-14 Identity = 81/322 (25.16%), Postives = 130/322 (40.37%), Query Frame = 1
Query: 379 NFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVS--INEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKIS-GMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSF-----------NIKF--DGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGN 1296
+F N T I + K + +TDKP+YKP +V F+ I P E I ++VE I+I+ P R+ QW ++ MN+ + +++ PIFG W FE++KYVLP+F V++ PS I + +V ++Y+ GKP+ K + M Y ++ DL +++ F + K NV G S + V G K +F +IK+ + R +KPGLPY G++ + + PA V + I + A N
Sbjct: 28 SFQNST-TITVEQKGSTVLIQTDKPVYKPKQRVLANFIIISP-----ELRPINEKVEA-----------------------YIVIQDPRGSRMVQWSDLHPICCGIMNVSFPISEQPIFGEWMIFVELHSHTYNKSFEVQKYVLPKFEVDISTPSFIRNLNVCDKARVRARYTFGKPVRGKATVNMTVNGVGY-------YRQDLGMPVLKTFEVDGAASFDIC-----VKDLMPPNVPEHFRGALSVWATVVASDGSKQITFDDSTLVHKQLVDIKYSRETRKQFKPGLPYHGKLEVTYPDGSPADGVTVRIKAELAAKDN 308
BLAST of Alpha-2-macroglobulin vs. Ensembl Sea Lamprey
Match: cd109 (CD109 molecule [Source:ZFIN;Acc:ZDB-GENE-120215-253]) HSP 1 Score: 261.536 bits (667), Expect = 8.634e-71 Identity = 258/968 (26.65%), Postives = 422/968 (43.60%), Query Frame = 1
Query: 2062 GPDVHVIAYFINTDNEVVADAL--EIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHK---------CIPQKRVKRMIQPIYGLE--YDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFS---YFNKTTKHLNLTVPDSMTTWRADSYCMTEK-GLWIAPT-QKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKIN--------VDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSE-LICNKNEQLGILS---VFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYL-EKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKL-LTAYVLIS-LNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWP--FDKES------SDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVF-AKTIGVSQNVNLKVSVVLNRNKTYNGLITLD----NRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNI-------HRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYS 4806
P HV+ +++ D EVV ++ ++A N L + + A P S V L V E + + D+++L L+K + IT DS + +P R P++ L DS + AG +T+A + + + G + F M+ V + A +++R FPE+W+++ +K TVPDS+T+W A ++ ++ GL +A T Q+L PF+ ++ +PYS+ E + VFNY T ++ V ++ VD NE+ I + + + D TV F + P ++G + +T+ + +D++ +Q+LV+ GV E +FS+ L+ ++ + VF PS +V GS R+ LT GD+MG + L++LV MPYGCGEQNM+ P+IYV+ YL + + +A S++ GY R+LTY+ DGSFSAFG D GSTWL+AFVL+ F A YV I+ + E +L ++ S G F E G++ + M GG + G L LTAY L++ L + + + N R+ T S + ++ +L +SV KYWP + ES E + D+ +L H + +++ W R L G S DTVV L AL+ F A+T G N+ ++ + +K G ++ N LV Q + I+ V++ G+G A QL+ YN+ R D F+L++ I N ++ VC + +S M L+ +N SG+ + K + R++ ++ LY D+ + G C + + + Q A V I DYY P +E+SYS
Sbjct: 423 APSAHVVVHYVRDDGEVVPASVTFDVAGIFENKVVL-----NWTAEMATPASSVSLKVSASDAEAFVGVLVVDKSVL----------------LLKTGNDITADSVTSAVSAMASTSRGGPFDAVPWGR----FMPLFWLPNPSDSLSTFTEAGLVLLTDAYVHHPEFFLVYKEGESGAGASYFRPLGGAMNMM--PVATGAGAAPAERVRTLFPETWIWADGETGKNRSKTFTATVPDSITSWVASAFSVSSSLGLGVADTAQQLLAFQPFFVSLNLPYSVVRGEQFVVQALVFNYLDTDLQVRVTLDPSDAYVVLVDTNEVGAIPNEQLVTVRSQ-DAATVSFPVSPALLGLVPITV---------------RARAATAADAMTQQLLVKAEGV--ERSFSKTLLLQRSGGDAVTQGSLVFPFPSGVVEGSQRATLTIIGDIMGPSISGLERLVQMPYGCGEQNMINFAPNIYVIKYLTATGQGGGEIRQRALSFMNTGYQRELTYQRQDGSFSAFGNEDASGSTWLSAFVLRCFLQALPYVFIDPVVVAETHVWLMSQLNGSTGVFAEPGRVIHADMQGG------SSGPLSLTAYALVALLEQPSHMTAHKRVLTLTQSERDKRVIRSGNLRILSTVTYALSRKHSPTENERK-TRLCIRSVKLDSLKYWPERGNNESMLVQLRPHETRPPGKDVRKISRLLLLTSVHLNKKNNVQITKWNLQSRTRLEGQSSLLDTVVGLQALSEFAARTTGSRSNLQVRAT-----SKLLTGPLSFTVNSANALVLQRAEVKACEIN-VALSATGAGFAVAQLNVFYNLKMSGGRAKRSADQTDAFELTIDPHI---LDLNNVDVNVCVRW-AGTGSSGMALLQVNMLSGFVADSDFPKPQV------VKRVEVDGGKVNLYLDSLN----GTLVCVTIRSTRVQTVGSAQEAQVSITDYYEPRRRTERSYS 1318 HSP 2 Score: 62.3882 bits (150), Expect = 1.227e-9 Identity = 93/435 (21.38%), Postives = 178/435 (40.92%), Query Frame = 1
Query: 382 FINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNV-SINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQ---FEIKKYVLPRFIVEVIPPSEILAKD-ESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVF---------ILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPY-YKPGLPYFGRINLVRHNKKP--------AKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQI--SEVSSNLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIV 1611
F+N + I + ++F +TDK +YKPG V R ++ S + +D I I+ P RV QW+ + S + ++ ++ ++ P+ G W+ + G++ + + F + +YVLP+F V + PS + +D + +S + +KY+ GK + G + G + ++ L +KL+ F ++A SF + +VT G + S +V+ P + +++F P +K GL Y ++L + P +V +S S S A ++W +NYT GV+ +P A S+ + + + +NL+ ++T + G +Q N K + L ++ + + + Q+ SRG IV
Sbjct: 24 FVNSSTLI-FDSQALLLFVQTDKAVYKPGQTVHFRAFSV----------------------------DSSLRPHLGGAYD-IEIQDPVGNRVQQWRGLTSASGVVSADFPVSDQPLLGQWQI--IVHGEQGVDKEQPFTVAEYVLPKFEVLLKLPSFVAREDGQGVSGTIEAKYTYGK----LVRGNATVTLIIGNPRPWEWQPSL-NLTIRNLKLDGNAGFAFSSDEVKALVADSFVPDTYTIEAHVTELLTGITQSGGGSVKTPEYR---VDVQFVNSPLSFKAGLNYTAYVHLSLPDDSPLLPRDLESPLLVQVSQSNLSSALLSSWE--QNYTVPASGVVAVHFSVLPDADSVYVNVVYKQQKNNLQLNKESST----------RSGESIQLSSANSKAQAGEPLQFSVTSTEHV--EEFFYQVVSRGNIV 404
BLAST of Alpha-2-macroglobulin vs. Ensembl Sea Lamprey
Match: cpamd8 (C3 and PZP like alpha-2-macroglobulin domain containing 8 [Source:ZFIN;Acc:ZDB-GENE-050208-428]) HSP 1 Score: 202.986 bits (515), Expect = 1.922e-52 Identity = 183/625 (29.28%), Postives = 290/625 (46.40%), Query Frame = 1
Query: 3205 NKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKL-LTAYVLISL--NGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKES----------SD--EQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVV---LNRNKTYNGLITLDNRLVSQTFNLGTENIS-YVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIR------------------------------------NIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEKEPGRPEVLLCPICVNDSINSTLKF 4914
N NE A+P +V GS R+ + GDVMG T +NLD L+ +P+GCGEQNM+ P+++VL YL + +A Y++ GY RQLTYK DGS+SAFG+ D GS WLTAFVLKSF + ++ I+ L A ++ Q+ DG F G++ + + GGI GK+ LTAYVL +L G D + +A N+ + Y AL++Y L+L S + +L + +++ K+W + SD Q SA++E T YA+L D++ ++ V WLS QRN+ GG+ STQDT VAL +L+ +A + VNL +S+ L+ +++ + DN+ + QT + + +VS K G GC +Q+ YN+ AA F+L+V E+ ++ + R ++VC +L+ +SNM ++ + SG+ E + + L L R + ++ Y+D +C +F + + + P VKI DYY P + + + Y N + N +E +C S N L+
Sbjct: 1088 NHNEAF----TLAVPHSVVPGSERATASMIGDVMGPTLNNLDNLLKLPFGCGEQNMIHFAPNVFVLKYLQQTKQLVADVRTEATDYLLQGYQRQLTYKRQDGSYSAFGERDSSGSMWLTAFVLKSFAQSRAFIYIDPKELAAAKAWIINHQKEDGSFPAVGRILNKDIQGGIH------GKISLTAYVLAALMETGTDNEGEKTAVARAKMFLESNMYSAEDPYTTALSAYALTLLHSSYAPV--ALRRLNNVAITQDGLKHWSLSGTTVTNDDTFMGFSDGLSQSVVSAEVEMTAYALLTYTALGDVATALPIVKWLSQQRNAQGGFSSTQDTCVALQSLSEYA-ILSYIGGVNLTISLASTNLDFQESFE--LNKDNKKILQTATIPSIPTGLFVSAK--GEGCCLMQIDVSYNVPDP-AAKPAFQLTV--ELTEPQRPHSRRHPQPLPLPHPGSASGSSDNDDDDPAAHHDSKEYKVTLEVCARWLQTG-SSNMAVLEVPLLSGFRADVESLEQVLLDKKLGLKRYELDGRKVLFYFDEIPNQCM---TCVKFHADREYIVGQTAPVPVKIYDYYEPALEATRFY---NASANSPLARE----------LCEGKSCNELLQL 1675 HSP 2 Score: 92.4337 bits (228), Expect = 9.359e-19 Identity = 101/435 (23.22%), Postives = 186/435 (42.76%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEA-LLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRK--HKCIPQKRVKRMIQPIYGLEYD----SRKVLSTAGFTAITNARIVNVKCMQ----YLARGMP---DRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTK----HLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANE--INVIGLKSFT--LCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMS--------------DSVRRQILVEPPGVPTETTFSELICNKNEQLGI 3228
+ IP+ M P ++ Y + + E VAD+++I + +++ + ++ P KV + V C + LL+ G + +K+ K + + G ++ H P +R I+ +D +R + AG T +T+ IV + Q + P + A FS + SR K+ R +FPE+W++ FN + L + VPDS+T+W A++ ++ +GL +AP + L+ PF+ + +PY++ E ++P+ V NY C E++VK+ V + G + T +C ++ KT L +G+ N+T + + + C E+ + + A D + R +LVE G+P E T+S C NE++ I
Sbjct: 531 LRIPVTHAMVPSSRLLVYHVRANGEGVADSVQIPVKPSFENEV---SLELSQNETSPGDKVDIVVRAAKGSCACVAAVDKSVHLLKPGYQLS------TEKVFKELAEFDVSESYGTSKEDSHFWWPGISSRRRRSSIFPWHWDITKDTRFAFTEAGLTVMTD--IVRLNHRQNGGMFTEEAGPAFQPHTGAFFSATAHGRAASR----------ADKRKRTFFPETWIWHCFNTSAATGEARLQVEVPDSITSWVAEALAISPAQGLGLAPARSLRTFKPFFVDFTLPYALIRGEQAKVPLTVHNYMPLCAEVHVKVTVPKGVKFVGHHGKQHLTRKMCVEADETKTTSLVLSFAELGQTNITARAFAYGEASCCEEGAQTLKGAKYVEEVAPERRTPMGLDYIMRSVLVEAEGMPREYTYSVFFC-PNERIHI 943 HSP 3 Score: 79.337 bits (194), Expect = 9.176e-15 Identity = 93/360 (25.83%), Postives = 147/360 (40.83%), Query Frame = 1
Query: 361 TKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVS--INEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKIS-GMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYS-KAKSQINVTVTENGTSSSVSKAVEGPGIKYNSF-----------NIKF--DGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTS---NKKGVIEFVIPYIP-LAQ 1377
+K+ +F N T I + K + +TDKP+YKP +V F+ I P E I ++VE I P R+ QW ++ MN+ + +++ PIFG W FE++KYVLP+F V++ PS I + +V ++Y+ GKP+ K + M Y ++ DL +++ A+SF K NV G S + V G K +F +IK+ + R +KPGLPY G++ + + PA V + I + A N YTS ++ G++ F I IP +AQ
Sbjct: 79 SKSDAFSFQNST-TITVEQKGSTVLIQTDKPVYKPKQRVLANFIIISP-----ELRPINEKVEAY-------------------------IVDPRGSRMVQWSDLHPICCGIMNVSFPISEQPIFGEWMIFVELHSHTYNKSFEVQKYVLPKFEVDISTPSFIRNLNVCDKARVRARYTFGKPVRGKATVNMTVNGVGY-------YRQDLGMPVLKTFEVDG------AASFDICVKDLMPPNVPEHFRGALSVWATVVASDGSKQITFDDSTLVHKQLVDIKYSRETRKQFKPGLPYHGKLEVTYPDGSPADGVTVRIKAELAAKDN------IYTSELVSRDGIVAFQIHSIPTMAQ 388
BLAST of Alpha-2-macroglobulin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004103.1 (pep scaffold:Pmarinus_7.0:GL476516:13600:100895:-1 gene:ENSPMAG00000003674.1 transcript:ENSPMAT00000004103.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 176.022 bits (445), Expect = 1.167e-44 Identity = 194/817 (23.75%), Postives = 369/817 (45.17%), Query Frame = 1
Query: 2650 IREYFPESWVFSYFN--KTTKH--LNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKT---------VYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDN------LDKLVVMPYGCGEQNMVAVVPSIYVLDYLNS--------LNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKM-QRSDGCFEEKGKLFSSTMAGGIKRNDKADGKL-LTAYVLISLN------GVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSF---GRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGL-ITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYN-IHRLYAANDTFKLSVK-TEIDSVKQCNIR------------------------NIKVCFAYLRR----DKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEKEPGRPEVLLCPICVND 4890
+R YFP+SW ++ + + KH + L +PD++TTW + +++ +G+ +A L F+ +++PYS+K E ++ V ++NY I ++++ V + S + K +K+ V F + P IG+ ++I + + D V++ + V P GV + S ++ + + P + + + ++++ GD + +T N + L+ +P GCGEQNM+ + P+ L YL+S LN + A+ ++ GY+R+L+Y+ D S++AF T STWLTAFV+K + A + ++ L +++ K Q SDG + E ++ M GG+ +G + +TA++LI + G + N ++ +A+ ++ Y +A+T+Y L+L P S + + + + L+ V+F GR +YW D+ S Q S + +E T Y +L + H+D + V WL+ QRN GG+ STQ T++AL A+A + +N+++ + +N + IT + R V + + +++K G G ++ + S++N + + TF L V TE + + R + +C Y R K S M ++ +N +G+ + + L + ++ + + + +Y D + ++ CF FKI++ + +QP + DYY+P+ + Y++ PG V L P+C ND
Sbjct: 106 MRSYFPQSWGWNKYKIPASGKHPQIRLQLPDTITTWNMQAVSISKTRGVCLADPLLLVSTKDFFIKLHLPYSVKRGEQTEIRVILYNYMEESLTILTEMDI------VDSICSTSKSGAKPSQKSTVKAKGAMVVSFPIVPLKIGEHQISI-------------RSRVYGRTFGDGVQKVLRVAPEGVRDILSESRIVHVEERDTFFIKNEISPDIVPNSEVLTFISVKGDELAETMVNTLDAKSISHLIRIPLGCGEQNMIYMAPTTLTLIYLDSVQEWEKIGLNRREEAI-----EFLKQGYSRELSYRKADHSYAAFLNTP--SSTWLTAFVVKVYSLAKSIIIVDNKELCGPVEWVIKHRQNSDGSYREDAPVYHREMQGGV---GGLEGHVSMTAFILIGIQQAQEYCGGTVLNYQQSMNRAVQFLASKVSGLQRMYTIAITAYALALQDPGSEAAHSSWKK-LESRVTFELNGR-RYWKADEASHGLQMS-TISVEATAYGLLTYLQHKDHESARGVVDWLTEQRNYGGGFGSTQSTILALQAMAQYKMDSSSQIPINVQLEITSPKNNFEKKMKITEETRFVQEPHKIPPGG--NITVKALGKGTFTLSIMSVFNKVAPSSKSCSTFDLKVTMTESNDGESPQGRFGWFDGKRRRKRDIGDEGGVEAVYRMNLCTRYKPRKEDLSKESGMTIIEVNMLTGFLPDKNDLIQLKDSVDKYISYYEITDSVLIIYLDKVP---STENYCFAFKIKQMLRSDMIQPVTASVYDYYSPQDKCTRFYNL------------PG-GYVELSPLCQND 872
BLAST of Alpha-2-macroglobulin vs. Ensembl Nematostella
Match: EDO33391 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSB9]) HSP 1 Score: 215.312 bits (547), Expect = 1.321e-59 Identity = 173/581 (29.78%), Postives = 290/581 (49.91%), Query Frame = 1
Query: 3151 LVEPPGVPTETTFSELICNK------NEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL--NGVDLXXXXXXXXXXXXXXXEN-ITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFG-RYKYWPFDKESSDEQ-----KSNSADIETTGYAILALINHQDLSVS----MKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNK-TYNGLITLDNRLVSQTFNLG-------------TENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKT------RMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYN 4776
+ +P GV E T+S IC + N+++ + ++P+ IV GS+ + ++ GD+MG + LD L+ +P GCGEQNMV P+I+V+ YLNS +S KA Y+ GY RQLTYK DGS+SAFG DK GS WLTAFVL+SF A +++ I+ + +++ K Q+ GCF+ GKLF++ + GG+ + LTA+V++SL GV ++ I + +N +T++ N LA + +++ + + + +FG +++ K + Q + S IE T YA++ALI S + + V WL+ QRN+LGG+ STQDT VAL AL+ +A + ++ +++ V + + ++ I+ +NRLV Q + + ++SY + +++S +H+ TF S + + C + +C ++ SNM ++ SG+++ EE L R+ F ++ ++ LY+D K C RF IE+ + N +PA VK+ DYY+
Sbjct: 1 IFQPEGVRKEYTYSSFICPRDTAGMFNDKIEV----SLPTNIVDGSVHATVSAIGDLMGPSLSGLDGLLRLPTGCGEQNMVKFAPNIFVMQYLNSTRQMTDDVSTKAVGYLRTGYQRQLTYKRKDGSYSAFGDRDKEGSLWLTAFVLRSFAQARQFIFIDDNETNGTKEWIMKKQQETGCFQPHGKLFNNALKGGV-----STPVTLTAFVVVSLLEAGVAPSDPVITKAMKCISSKKNSVTDSYSNAILAFAHTIAGHADRDVYIARLKAKAIFEGTPTFGFGFRFLTPSKSIKNTQIIVFVRHASTKIEMTSYALMALIGDGSDSNAVADVLPVVRWLTQQRNALGGFSSTQDTCVALQALSRYAGHV-FGESTRMRIDVGFSGGQFSHQFRISDNNRLVLQRVVVSPYGNDNNRNDNDDSTDVSYNVFEVKEEPAFDLKVS----VHQ--RTGQTFTTSATETGEEL--CYPLELNIC----AKEDASNMAVIETKLVSGFKVDEES-----------LERVSFIQSYTLDGKKVVLYFDEVGI----KHVCVRFGIEQTNVVKNTKPASVKVYDYYD 544
BLAST of Alpha-2-macroglobulin vs. Ensembl Nematostella
Match: EDO32956 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STJ8]) HSP 1 Score: 181.415 bits (459), Expect = 3.509e-48 Identity = 109/345 (31.59%), Postives = 179/345 (51.88%), Query Frame = 1
Query: 2818 PFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDA-----NEINVIGLK--------SFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELI----------CNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTW--LTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL 3777
PF+ ++ +PYS+ E + + VFNY E+ + + NE++ G + + N+ + V F + PK +G + + + + +++ +D+V+R ++VEP GV E +++ L+ K +++ +L PS + GD+MG +F NLD L+ MPYGCGEQNMV P+I+VL YL ++ PA+ KA+ ++I GY R+ TY+H +GS+SAFG+ DK GS W LTAFV+KSF A KY+ I+ L ++ ++ Q G F + G + SS + GG++ LTA+V+I+L
Sbjct: 3 PFFVSLDLPYSVIRGEEVAIKALVFNYLQQPQEVTITLKASKHWTIINELSPRGAPDQGEPKDTEYKMTVPANEGRAVSFPITPKTLGTIPIIVQAQ---------------SVSAADAVQRILIVEPEGVEQEYSYTVLVDLNQSKYDHVTEKRKKIYMLFFHEFPS----------FSCTGDIMGSSFSNLDNLLRMPYGCGEQNMVNFAPNIFVLKYLTAVKQLTPAIRNKAEVFMIKGYQREQTYRHPEGSYSAFGERDKEGSMWYVLTAFVVKSFAQARKYIYIDEKALDQSIFWMVSKQSPSGAFPKVGTVHSSYLKGGLQSEIA-----LTAFVVIAL 317 HSP 2 Score: 73.9442 bits (180), Expect = 3.287e-13 Identity = 38/90 (42.22%), Postives = 59/90 (65.56%), Query Frame = 1
Query: 4015 KSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLD 4284
+ SADIE T YA+LA+ +++D+ + V WLS QRNSLGGY STQDTVV + A++ FA I S +++ V++ + T++ T++
Sbjct: 406 RPRSADIEITAYALLAIGHNKDVQTGLPVVRWLSQQRNSLGGYSSTQDTVVGIQAMSEFASFI-YSPSMDYTVTLTNTDDNTFSKTFTVN 494
BLAST of Alpha-2-macroglobulin vs. Ensembl Nematostella
Match: EDO38193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDN3]) HSP 1 Score: 186.808 bits (473), Expect = 2.649e-47 Identity = 158/590 (26.78%), Postives = 274/590 (46.44%), Query Frame = 1
Query: 2035 ISIPLMKNMGPDVHVIAYFINTDNEVVADA--LEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPI-----YGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTK---HLNLTVPDSMTTWRADSYCMT-EKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIG----LKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELI-----CNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNL-----DKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKY--VNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIK 3723
++ P+ M P V+AY++ NEVVAD+ +E+ + N L + K +P S V + + E G + ALL +D + LLRK+ + K+V + ++ + G DS V + AG + I+N + L G Y R + +K +N+ ++ D + + QIR+YFPE+W+F + L T+PD++TTW + ++ E G + ++ F+ + +PYS + E + + VFNY E+ V + D + G + TL ND +V F + P +G++ + I + ++ + + D+V + +LV P GV +S +I + +Q+ + A+P V GS+ + L F G+++G NL + L+ +P GCGEQNM+ + P++Y+L +L++ + AKA I GY +QL ++ D SFSAFG++ + GSTWLTAFV+K F A ++ V I+ ++++ +L QR DG F E + ++ G +K
Sbjct: 601 LAFPVTLAMIPRARVLAYYVTHTNEVVADSALMEVENWFPNKVWL---QVPTPAKGHLPGSTVEVKLH---AEAGSRV-----ALL------------------------GVDESVYLLRKNSRLTAKQVFQAVRDLDLGCGVGPGKDSSHVFNNAGLSIISNNNLA-------LKFGHEIEVY------RERQTKEKNARVE-EDIENQGQIRKYFPETWIFDEYQTGLDGKLALRYTLPDTITTWAVQALAVSNETGFGVTELSRVTTFKNFFIALLLPYSAQRGEQISVKAAVFNYGEQESEVEVHLTGDPTYCSSSGPGASSQPITLRIAGNDAISVSFVVVPVRLGRIPIRIVAKARLAGSHGDDWTM-------DAVEKDLLVVPEGVERRVVYSLVIDPQDVTHDGKQISNVE-LAVPKGAVPGSIFASLYFTGNLIGSAVYNLMTGGVEHLLRIPIGCGEQNMITLAPNVYILAFLSNSKQVTAEIEAKAYRLIQQGYQQQLGFRRKDKSFSAFGES-RPGSTWLTAFVIKVFCTAKRFDGVAIDNDLIRDSITWLLANQREDGAFPEVMDVVHVSLMGSLK 1132 HSP 2 Score: 83.5741 bits (205), Expect = 7.990e-16 Identity = 77/314 (24.52%), Postives = 127/314 (40.45%), Query Frame = 1
Query: 382 FINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAM-NLEYQLNKDPIFGIWKAKAVFDG----QEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFR---YSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNI-KFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGN 1296
FI ETR + + ++ ++ + DKPIY P V++R + + D + +++ +D II++ P +R W+ +S + + L + G W + G + FE+K+YVLPRF V +I P IL K SI V SKY+ GK ++ + + G + F S K K L+ FI R + QI V E T S F++ D ++KPGLP+ +I + NK+PA+ + + +S + N
Sbjct: 120 FIRETRVL-ISYRSMMLLVQIDKPIYTPSQTVKMRVIPL---------DFDLQSLKVKVD---------------------IIVKNPQGIRTQNWQKLSSEHGIISKALSLGPRAMLGNWTIISYHHGYGNKTNTTVHFEVKQYVLPRFSVRIIGPDYILPKTRSIKIAVISKYTYGKAAQGSVTVRLSV--TGGGSPSIRFYSTTEKLIDGKSTLDVDSGFIKKHPSRPWFPDGRRLQIECDVIEEATGMRESAVDNSVYFVSTPFHVTTADTALFFKPGLPFVVKIAIHYPNKQPARGIPVDVSAMGKKHNN 400
BLAST of Alpha-2-macroglobulin vs. Ensembl Nematostella
Match: EDO33393 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSC1]) HSP 1 Score: 159.073 bits (401), Expect = 1.455e-43 Identity = 79/179 (44.13%), Postives = 115/179 (64.25%), Query Frame = 1
Query: 3241 AIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISL 3777
++P+ IV GS+ + ++ GD+MG + LD L+ +P GCGEQNMV P+I+V+ YLNS +S KA Y+ GY RQLTYK DGS+SAFG DK GS WLTAFVL+SF A +++ I+ + +++ K Q+ GCF+ GKLF++ + GG+ + LTA+V++SL
Sbjct: 32 SLPTNIVDGSVHATVSAIGDLMGPSLSGLDGLLRLPTGCGEQNMVKFAPNIFVMQYLNSTRQMTDDVSTKAVGYLRTGYQRQLTYKRKDGSYSAFGDRDKEGSLWLTAFVLRSFAQARQFIFIDDNETNGTKEWIMKKQQETGCFQPHGKLFNNALKGGV-----STPVTLTAFVVVSL 205
BLAST of Alpha-2-macroglobulin vs. Ensembl Nematostella
Match: EDO33392 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSC0]) HSP 1 Score: 82.0333 bits (201), Expect = 5.911e-18 Identity = 45/141 (31.91%), Postives = 79/141 (56.03%), Query Frame = 1
Query: 2644 KQIREYFPESWVFSYFN----KTTKHLNLTVPDSMTTWRADSYCMTE-KGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKL 3048
+++R+ FPE+W+++ N K + VPD++T+W A ++ M+ G+ +AP+ L V PF+ + +PYS+ E + + V VFNY C I + + D E++ +S +C C N+ K+V F L PK +G +
Sbjct: 1 ERVRKNFPETWLWTEENVNNVTGEKVITAQVPDTITSWYASAFAMSSTHGIGVAPSSSLTVFKPFFVSFTLPYSVIRGERVSVIVSVFNYLSQCLTIRINLAKSDFFELHSADNRS--VCVCGNEAKSVQFTLTPKKLGPM 139
BLAST of Alpha-2-macroglobulin vs. Ensembl Medaka
Match: ENSORLT00000030023.1 (alpha-2-macroglobulin-like protein 1 [Source:NCBI gene;Acc:101161539]) HSP 1 Score: 369.007 bits (946), Expect = 2.060e-104 Identity = 285/954 (29.87%), Postives = 466/954 (48.85%), Query Frame = 1
Query: 2035 ISIPL--MKNMGPDVHVIAYFINTDNEVVADA--LEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRM-IQPIYGLEYDSRKVLSTAGFTAITNA-RIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVF---SYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKI-NVDANEI-NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQD-----LSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNI-RNIKVCFAYLR---RDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNK 4830
I IPL + ++ P V+ Y I + EVVAD+ I LNN L KF+ +P +K LT+ + C++R D+++L + Y+ +L + D ++ + C P+ + + ++P + K S F + I V LA+ A + QMS N+ ++ SD + K+ +R YFPE+W++ S + + L TVPD++T W A ++C++ G +AP L PF+ ++ +PYS+ EV L VFNY C + + N D N G ++C C + KT + + P +G + + + + ++ S+ K + + D+V +LVE GVP + + L+C +S+ +P + V+GS R+ ++ GD+MG+ NLDKL+ MPYGCGEQNM+ P+I++LDYL S + A Y+ GY R+L YKHDDGS+SAFG +D G+TWLT FV+KSF+ A +Y+ ++ +KEA K+L +Q+ DGC + GKLF S M GG+ +D LTAYV ++ +D T + + C + + N + +F L +S + G ++W ++ + E +S ++E T Y +LAL++ L + V WL+ Q+N GG+ STQDTVVAL ALA +A T + + V+ V NK + + DNRL+ Q L TE +++ G C Q+S YNI + F++S T +C+I R + F ++ R + +NM+++ I SG+ L + K L + + R+D ++ + +Y D G+ F +EE ++ NL+PAVVK+ DYY S+++ + + CNK
Sbjct: 479 IKIPLTNVIDLAPIAQVVVYTIVSSGEVVADSRDFPIQLCLNNKVSL-----KFSAVQELPGAKTTLTLR-AQPSSLCSVRAIDQSVLLLKSEEELTVDYVYNQL-PIKKMSGYDYQVEDNSFNPCFPRPWPRPIPLEPEFQPRGRRDKRASIFPFYEGDDVYTIFKVNYFTSLAK-------ADLAPAPEQMSS--NTDVQPSDTQKKETVRTYFPETWIWDLVSVGSNGSTSLVKTVPDTITKWAAGAFCVSPAGFGVAPNVGLTAFQPFFVSLTLPYSVVRGEVFTLKATVFNYLPKCIMVKATLANSDQFTFKNCDGCVYSSVCLCAEESKTFSWIVTPTALGDVTLKVSAEAEKSNKKCGKGVVTVPTVGQVDTVITTLLVEAEGVPQMVSHNALLCPARPVSKNISL-ELPEDFVNGSERASVSVVGDLMGRAMKNLDKLLAMPYGCGEQNMLLFAPNIFILDYLKSTGQLKESTRKTAVGYLESGYQRELNYKHDDGSYSAFGMSDPSGNTWLTTFVMKSFNAAKQYIFVDQMYIKEARKWLSGLQKPDGCIQSVGKLFHSGMKGGV-----SDDVSLTAYVAAAMLELDGNATDTVVQNCLECVKTAVADEITNLYTVALLSYTFTLAGDQDVRSKLFTLLDQKSTNEGGTRHW--KRDDAPETGLDSVEVEMTSYVLLALLSGPSLPGFGLDYAAGIVRWLAQQQNPFGGFSSTQDTVVALQALAKYATATFSPEGSTTVTVTSVGGLNKRFT--VNEDNRLLYQEEKL-TEIPGEYTIEAEGQSCVLAQISLFYNIPPP-SDFSAFQISTNTS----GRCDIPRPEFMLFVHVEFQGRREETNMVVINIKLLSGYILQDNSLKMLKE--NRLVKRVDVEEGYINIYLDGLK---KGQKETFDVILEEDQQVGNLKPAVVKVYDYYQT---SDEAVTDYTSPCNK 1392 HSP 2 Score: 70.4774 bits (171), Expect = 2.203e-11 Identity = 73/292 (25.00%), Postives = 126/292 (43.15%), Query Frame = 1
Query: 424 FIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEA-MNLEYQLNKDPIFGIWKAKAVFDGQEEI-IQFEIKKYVLPRFIVEVIPPSEILAKDESI-SFKVCSKYSTGKPMNAKISGMFC------MLYSYGKKK--CVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKPAKMVNI 1263
F+ +TDKPIYKPG V+ R +++ TN M + + V +LD P++ R+AQW + +++ ++L + + GI+ A D E I FEIK+YVLP+F V V PS I D+ + + +Y+ GKP+ + FC + +S ++K C F+ K C +E + + F + ++ + E GT + K I F+ P +KPGLP G++ ++ + +++ I
Sbjct: 93 FVHIVQTDKPIYKPGQTVQFRIVSM-DTNFM----PVNRTVCFLLD--------------------------PNSNRIAQWLDKNVDGGILDLSHPTIPEAAQGIYVISAETDEGETISYNFEIKEYVLPKFEVTVNLPSVITILDKELCPCALSCRYTYGKPVVGSVKAEFCRRSYQFIWFSLSEQKDICKTFQLTTDKSGCATQTVELADFALDSYGF---QDTFEVTTELEEFGT-GVILKGSGSASFTTQIRTISFEDVPSSFKPGLPLNGKVIVLIKIRNDNRIIQI 349
BLAST of Alpha-2-macroglobulin vs. Ensembl Medaka
Match: ENSORLT00000001261.2 (alpha-2-macroglobulin-like protein 1 [Source:NCBI gene;Acc:101161037]) HSP 1 Score: 345.125 bits (884), Expect = 1.223e-96 Identity = 276/967 (28.54%), Postives = 459/967 (47.47%), Query Frame = 1
Query: 2035 ISIPLMK--NMGPDVHVIAYFINTDNEVVADALE--IASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYL-----VQKLIKLNHKITLDS----------RIGLLRKHKCIPQKRVKR-MIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKH---LNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEI----NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQD-----LSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNI-RNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNK 4830
I IPL N+ P V+ Y I + EVVAD+ + I LNN L KF+ +P +K LT+ + C++R D+++L + Y+ ++K+ ++++ DS R L + ++R KR I P Y E D + AI A + +V + +P + + + + IR YFPE+W++ + + L TVPD++T W A ++C++ G +AP L PF+ ++ +PYS+ EV L VFNY C I VK + ++ N G ++C C + KT + + P +G + + + + ++ S+ + + + D+V +LVE GVP + + L+C +S+ +P + V+GS R+ ++ GD+MG+ NLDKL+ MPYGCGEQNM+ P+I++LDYL S + A Y+ GY R+L YK DDGS+SAFG +D G+TWLT FV+KSF+ A +Y+ ++ + +++A +L +Q+ DGC + GKLF + M GG+ +D LTAYV ++ +D T + + C ++ N + + L +S++ G ++W ++ + E +S ++E T Y +LAL++ L + V WL+ Q+N GG+ STQDTVVAL ALA +A T + + V+ V NK + + +NRL+ Q L TE ++K G C Q+S YNI + F++S T +C+I R F + R + +NM+++ I S L + K L + R+D ++ + +Y D G+ F +E+ ++ NL+PAVVK+ DYY S+++ + + CNK
Sbjct: 497 IKIPLTNVINLAPIAQVVVYTIVSSGEVVADSRDFPIQLCLNNKVSL-----KFSAVQELPGAKTTLTLR-AQPSSLCSVRAIDQSVLLLKSEEELTVDYVYNQLPIKKMWGYDYQVEDDSSNPCFPRPWPRPIPLEPEILLRERRDKRSRILPFY--EGDDVYTIFKVKPLAINAANLESV-----MNEPVPAPA---------------------PEGQKTETIRTYFPETWIWDLVSVGSSGSTSLVKTVPDTITKWAAGAFCVSPAGFGVAPNVGLTAFQPFFVSLTLPYSVVRGEVFTLKATVFNYLPKC--IMVKATLAKSDQFTFKNCDGCVYSSVCLCAEESKTFSWIVTPTALGDVTLKVSAEAERSNKKCGTEVVTVPTVGHVDTVITTLLVEAEGVPQMVSHNALLCPARPVSKKISL-ELPEDFVNGSERASVSVVGDLMGRAMKNLDKLLAMPYGCGEQNMLLFAPNIFILDYLKSTGQLKESTRKMAVGYLESGYQRELNYKRDDGSYSAFGMSDPSGNTWLTTFVMKSFNAAKQYIFVDQTYIEDARTWLSGLQKPDGCIQSVGKLFHNGMKGGV-----SDDVSLTAYVAAAMLELDGNATNPVVQNCLKCLRTAVSGKITNLYTVALLSYTFTLAGDQDMRSKLITLLDQKSINEGGTRHW--KRDGAPETDLDSVEVEMTSYVLLALLSGPSLPGFGLDYAAGIVRWLAQQQNPFGGFSSTQDTVVALQALAKYATATFSPEGSTTVTVTSVGGLNKRFT--VNKNNRLLYQEEKL-TEIPGEYTIKAEGQSCVLAQISLFYNIPPP-SDFSAFEISTNTR----GRCDIPRPEFTLFVFQGRREETNMVVINIKLLSACVLFVSQLKENRL-----VKRVDVEEGYINIYLDGLK---KGQKETFNVILEKDQQVGNLKPAVVKVYDYYQ---TSDEAVTDYTSPCNK 1400 HSP 2 Score: 91.2781 bits (225), Expect = 8.053e-18 Identity = 100/392 (25.51%), Postives = 166/392 (42.35%), Query Frame = 1
Query: 175 IVLTIESIHDPSQIKHQSSKFVIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEA-MNLEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC-----MLYSYGKKK---CVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKP----AKMVNISISIQSWAYGNNWG 1305
+ +T++ ++I S K I + FQ + VP V S + I + I G + + +N+ ++ + F+ +TDKPIYKPG V+ R +++ TN + +G + ++ K D +SN R+AQW + SI+ ++L + + GI+ A D E I FEIK+YVLP+F V V PS I D+ ++ K+C KY+ GKP+ + FC + K+ C F+ K C + I A ++ S ++ + E GT + KA + F+ P YKPGLP G+I + + P A + + S++S Y + G
Sbjct: 41 LTITLDVDSSSTKIGQLSFKTEIYRCFQ---FLVPIVSSTTVASISVKIQGKM---------------DSLNQETKVLIEPPAFVHIVQTDKPIYKPGQTVQFRIVSM-DTNFIPVNRMVGFYIHVL------------KQDPNSN-------------RIAQWLDKSIDGGILDLSHPTIPEAAQGIYVISAETDKGETISHNFEIKEYVLPKFEVTVNLPSVITILDDEVTLKICGKYTYGKPVLGSVKAEFCRRANQFFWLPPPKQDDICKTFELSTDKSGCATQNVALAGFAINAYNYFDS---FEVTAELEEFGT-GVILKASGSASFTTQIRTVSFEDVPSSYKPGLPLKGKIKVTGADGSPIANQAVYLYVVDSLESTLYTDQKG 384
BLAST of Alpha-2-macroglobulin vs. Ensembl Medaka
Match: ENSORLT00000012489.2 (alpha-2-macroglobulin-like protein 1 [Source:NCBI gene;Acc:101159604]) HSP 1 Score: 343.969 bits (881), Expect = 4.848e-96 Identity = 273/973 (28.06%), Postives = 477/973 (49.02%), Query Frame = 1
Query: 2056 NMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMP--KSKVVLTVDPGVKENGCAIRIADEALL--QFGQSSNADLSYLVQKLIK--LNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIY------GLEYDSRKVLSTAGFTAITNARIVNVKCMQYLAR----GMPDRS--YATFSNERTQMSK----------------SRNSVLKISDAKMK--KQIREYFPESWVFSYFN------KTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDAN-EINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALIN----HQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAK---TIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEID-SVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPL-NRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFN 4815
+M P V ++ Y I E +A + +++G +C + +F+ +A+P ++++ LT P ++ C + D+++L + G + +AD Y + L + + ++I +C+P R KR I P Y G + D+ + AG TN + C+++ R GM Y N MS+ + SV DA + + +R +FPE+W++ KT H TVPD++TTW +++C++ +G +AP QK+ V PF+ + +PYS+ E +L VFNY +C + V ++ + + +T C C N+ KT+ +NL +G +NVT+ + + S + + K+ D V + ++V+ GV ++ L+C L + +P ++ GS R ++ GD++G+ NLD L+ MPYGCGEQNM + P IY+L YL S P ++ K ++ GY RQL YK +G+++ FG G+TWLTAFVL+SF+ A ++ I+ +KE+ ++LE Q +GCFE+ GKLF++ M GG+ +D L+AY+ + + ++ + K++ C ++++ + Y + ++ QL + ++ G + +W + +KS+S +E T Y ++A + +DL + V WLS Q+N GG+ STQDTVVAL ALA+++ T G S V L+ + T++ + DN+L+ Q L SY +++ GS CAS+Q+S YNI N T + V E D S K+ + +K+ Y ++ N+NM+++ I SG+ + + S+ PL +R++ + + +Y + +E++ + NL+PAVV + DYY P +E Y+ N
Sbjct: 540 DMAPTVQIVVYAILPSQEFIAHYVSLSTG--DCFK-NEVSVEFSPSSAVPGEEAEMQLTAHP---DSLCGVSAIDQSVLIKEPGGTLDADKIYNLLPLRRTYIPYEIQ--------EVPECLP-VRPKRYISPDYIHPPNIG-QSDAYSMFQNAGLKVATNLILQVPTCLKFKGRMYHTGMAGGIGLYRDGLNGPPLMSRMGAPPGLPGAVGAAPEAMASVAYDYDAGPEPVETVRTFFPETWIWDLVEVGESGKKTVAH---TVPDTITTWETETFCLSSQGFGLAPRQKITVFQPFFLELTLPYSIIRGENFELKATVFNYLSSCIMVEVTPTPSSDYTLTALSSDQYTSCLCGNERKTLRWNLASIALGVMNVTVSAEAVPSHASCDNEIVKVPERGRIDKVTKSLIVKAEGVEVTKAYNWLLCPNGNTLTETTEIQLPDNVIEGSARGTVSVLGDILGRALKNLDGLLQMPYGCGEQNMALLAPDIYILHYLKSTKQLTPEINEKVSQFLKSGYQRQLNYKDSEGAYTTFGSGP--GNTWLTAFVLRSFYKAQSFIYIDEEKIKESRRWLEGKQMQNGCFEQSGKLFNNRMKGGV-----SDEVTLSAYITAAFLEMKISPNDHVLNKSLLCLKSSLSDLSNTY-TTALLAYVFTLAEDMETRDHLLKQLDNSAIREGGFLHW----SQTGTEKSDSLSVEITSYVLMAKLGASPTSEDLGYASSIVRWLSGQQNYYGGFSSTQDTVVALQALALYSSLVFTPGGSSTVTLQSP---SGQLTFH--VNPDNKLLYQESVLQKMTGSY-NLEVKGSACASVQISLFYNIPTPQETN-TLSIKVLPEADLSTKKPRVI-LKLNSLYTGKETNTNMVIIDITILSGFVPNVKSLDSIK---KAPLVDRVEHKDGHVLVYLRELVKELPMN---YSLVLEQQHVVQNLKPAVVTLYDYYQPSERAEAEYTFQN 1467 HSP 2 Score: 66.6254 bits (161), Expect = 2.707e-10 Identity = 75/301 (24.92%), Postives = 124/301 (41.20%), Query Frame = 1
Query: 433 FAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKN-VSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFC---MLYSYGKKKCVAFKSDLMKQNC-NKIKLESK-----IVFILASSFRYS----KAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKP-AKMVNISISIQSWA 1287
F +TDKPIY PG V R A+ SK S F++I ++ P R+ QW N S + + L Y L+ + G+ + + F+++KYVLP+F V + E+ E I+ +VC+KY+ G+P+ IS C + YG S ++ + C KIK K + F +++ R + + ++ V E GT S+ +A + + Y + F P Y G++ V +N P A M +S + W+
Sbjct: 128 FVQTDKPIYLPGQMVNFRVFAL-----------------------------DSKLRPASLLFNVIELQDPHGNRIGQWLNETSDGKILALSYSLSSEAREGVHQIVLSVGDDKISHSFKVEKYVLPKFEVTLNVKEEVSIGQEEITAEVCAKYTFGQPVPGTISVKMCRPSQPFYYGVTVKKEDGSPVITEPCVGKIKQTDKSGCTTLEFSMSTFTRMDAKILRDELKVEALVEEEGTEISLGQA-KTIQLSYVIGKLSFTDTPKLYNQNSTLEGKVKAVYYNDTPIAGMQLHVLSGRMWS 398
BLAST of Alpha-2-macroglobulin vs. Ensembl Medaka
Match: ENSORLT00000000908.2 (pep primary_assembly:ASM223467v1:2:10066528:10081498:1 gene:ENSORLG00000025635.1 transcript:ENSORLT00000000908.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 339.732 bits (870), Expect = 6.152e-95 Identity = 264/956 (27.62%), Postives = 459/956 (48.01%), Query Frame = 1
Query: 2020 IFERIISIPL--MKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKH---LNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEI---NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQD-----LSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNI-RNIKVCFAYLR---RDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNK 4830
+ + I IPL + ++ P V+ Y + + EVVAD+ + L C L F+ +P +K LT+ + C++R D+++L + Y+ +L + D ++ + C + + + L R +S N K ++ + +T+ E M + + + + IR YFPE+W++ + + L +VPD++T W A ++C++ G +AP +L PF+ ++ +PYS+ EV L VFNY C + + V +++ N G ++C C + KT + + P +G + + + + ++ S+ + + + D+V +LVE GVP + + L+C +S+ +P + V+GS R+ ++ GD+MG+ NLDKL+ MPYGCGEQNM+ P+I++LDYL S + A Y+ GY R+L+YK DGS+S FG +D+ G+TWLT FV+KSF+ A +Y+ ++ + +++A +L +Q+ DGC + GKLF + M GG+ ND + LTAYV ++ +D T + + C ++ N + + L +S++ G ++W ++ + E +S ++E T Y +LAL++ L + VYWL+ Q+N GG+ STQDTVVAL ALA +A T + + + V+ V NK + + DNRL+ Q L TE ++K G C Q+S YNI + F++S T +C+I R + F ++ R + +NM+++ I SG+ L + K S + R+D ++ + +Y D G+ F +EE ++ NL+PAVVK+ DYY S+++ + + CNK
Sbjct: 472 VIKGTIKIPLANVTDLAPIAQVVVYTMISSGEVVADSRDFPIQL--C--LNNKVLNFSAVQELPGAKTTLTLR-ARSGSLCSVRAIDQSVLLLKSEEELTVDYVYNQL-PIKKMWGYDYQVKDHPFNHCFSRPWPRPIPLEPALLPRGRRDTVSHHSPFKKGNDVYTIFKVNGFIVFFC---ALSTWPLEIFGMQSGMSMPAPAPEGEKTETIRTYFPETWIWDLVSVGSSGSTSLVKSVPDTITKWAAGAFCVSPAGFGVAPNVRLTAFQPFFVSLTLPYSVVRGEVFTLKATVFNYLPKCIMVKATL-VKSDQFTFKNCDGCVYSSVCLCAEESKTFSWIVTPTALGDVTLKVSAEAEKSNKKCGTEVVTVPTMGQVDTVITTLLVEAEGVPQMVSHNALLCPARPVSKKISL-ELPEDFVNGSERASVSVVGDLMGRAMKNLDKLLAMPYGCGEQNMLLFAPNIFILDYLKSTGQLKESTWKMAVGYLESGYQRELSYKRYDGSYSPFGMSDRSGNTWLTTFVMKSFNAAKQYIFVDQTYIEDARTWLSGLQKLDGCIQSVGKLFHNGMKGGVS-NDVS----LTAYVAAAMLELDGNATNPVVQNCLKCLRTAVSGKITNLYTVALLSYTYTLAGDQDMRSKLVTLLDQKSINEGGTRHW--KRDGAPETGLDSVEVEMTSYVLLALLSGPSLPGFGLDYAAGIVYWLAQQQNPFGGFSSTQDTVVALQALAKYATATFSLEGSTTVTVTSVGGLNKYFT--VNKDNRLLYQEEKL-TEIPGEYTIKAEGQSCVLPQISLFYNIPPP-SDFSAFQISTNTS----GRCDIPRPEFMLFVHVEFQGRREETNMVVINIKLLSGYILQDNSLKME----SRLVKRVDVEEGYINIYLDGLK---KGQKETFDVILEEDQQVGNLKPAVVKVYDYYQT---SDEAVTDYTSPCNK 1391 HSP 2 Score: 73.9442 bits (180), Expect = 1.533e-12 Identity = 62/216 (28.70%), Postives = 96/216 (44.44%), Query Frame = 1
Query: 634 ETPDNVRVAQWKNVSINEA-MNLEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCM---LYSYGKKK-----CVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKS-QINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRP-YYKPGLPYFGRINLVRHNKKP 1245
+ P++ R+AQW + SI+ ++L + + GI+ A D E I FEIK+YVLP+F V V PS I D+ ++ K+C KY+ GKP+ + FC YS+ C F+ K C + + Y+ S ++ + E GT + KA + F+ P YKPGLP G+I + + P
Sbjct: 125 QDPNSNRIAQWLDKSIDRGILDLSHPTIAEAAQGIYVISAKTDKGETISHNFEIKEYVLPKFEVTVNLPSVITILDDEVTLKICGKYTYGKPVLGSVKAEFCRHLNQYSWLPSPKQDYICKTFELSTAKNGCAT----QNVALAGFAMNTYNDFDSFEVKAELEEFGT-GVILKASGSASFTTQIRTVSFEDLPSSYKPGLPLEGKIKVTGADGSP 335
BLAST of Alpha-2-macroglobulin vs. Ensembl Medaka
Match: ENSORLT00000037305.1 (alpha-2-macroglobulin-like protein 1 [Source:NCBI gene;Acc:111948476]) HSP 1 Score: 332.798 bits (852), Expect = 4.586e-93 Identity = 266/955 (27.85%), Postives = 450/955 (47.12%), Query Frame = 1
Query: 2035 ISIPL--MKNMGPDVHVIAYFINTDNEVVADA--LEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYL-----VQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRM-IQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKH---LNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEI---NVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQD-----LSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFA-KTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNK 4830
I IPL + ++ P V+ Y I + EVVAD+ I LNN L KF+ +P +K LT+ + C++R D+++L + Y+ ++K+ + ++++ DS + C P+ + + ++P E R++ T V + L G D Y F + + + + + IR YFPE+W++ + + L TVPD++T W A ++C++ G +AP +L PF+ ++ +PYS+ EV L VFNY C + + V +++ N G ++C C + KT + + P +G + + + + ++ S+ + + + D+V +LVE G P S +P + V+GS + ++ GD+MG+ NLDKL+ MPYGCGEQNM+ P+I++LDYL S + A Y+ GY R+L YK DDGS+SAFG D G+TWLT FV+KSF+ A +Y+ ++ +++A +L +Q+ DGC + GKLF + M GG+ +D LTAYV ++ +D T + + C ++ N + + L +S++ G ++W ++ + E +S ++E T Y +LAL++ L + V WL+ Q+N GG+ STQDTVVAL ALA +A T + + V+ V NK + + DNRL+ Q L T+ ++K G C Q+S YNI + F++S T QC+I + R + +NM+++ I SG+ L + K L + + R+D ++ + +Y D + + F +EE ++ NL+PAVVK+ DYY S+++ + + CNK
Sbjct: 403 IKIPLTNVIDLAPIAQVVVYTIVSSGEVVADSRDFPIQLCLNNKVSL-----KFSAVQELPGAKTTLTLR-AQPSSLCSVRAIDQSVLLLKSEEELTVDYVYNQLPIKKMWEYDYQVEDDSF------NPCFPRPWPRPIALEP----ELLPRELRDT-------------VSHIFPLYEG--DDVYTIFKVKSFTFLINFCMAAPAPEGEKTETIRTYFPETWIWDLVSVGSSGSTSLVKTVPDTITKWTAGAFCVSPAGFGVAPNVRLTAFQPFFVSLTLPYSVVRGEVFTLKATVFNYLPKCIMVKATL-VKSDQFTFKNCDGCVYSSVCLCAEESKTFSWIVTPTALGDVTLKVSAEAEKSNKKCGTEVVTVPTLGQVDTVITTLLVEVKG-PVSKKIS---------------LELPEDFVNGSEGASVSVVGDLMGRAMKNLDKLLAMPYGCGEQNMLLFAPNIFILDYLKSTGQLKESTQKMAVGYLESGYQRELNYKRDDGSYSAFGMGDPSGNTWLTTFVMKSFNAAKQYIFVDQKYIQDARTWLSGLQKPDGCIQSLGKLFHNGMKGGV-----SDDVSLTAYVAAAMLELDGNATNPVVQNCLKCLRTAVSGKITNLYTVALLSYTFTLAGDQDMRSKLITLLDQKSINEGGTRHW--KRDGASETDLDSVEVEMTSYVLLALLSGPSLPGFGLDYAAGIVRWLAQQQNPFGGFSSTQDTVVALQALAKYATATFSPEGSTTVTVTSVGGLNKRFT--VNKDNRLLYQEEKL-TQIPGEYTIKAEGQSCVLAQISLFYNIPP-PSDFSAFEISTNTR----GQCDIPRPEFTLFVEGRREETNMVVINIKLLSGYILQDNSLKMLKE--NRLVKRVDVEEGYINIYLDGVR---SFEKETFNVILEEDQQVGNLKPAVVKVYDYYQ---TSDEAVTDYTSPCNK 1286 HSP 2 Score: 69.3218 bits (168), Expect = 4.161e-11 Identity = 47/163 (28.83%), Postives = 79/163 (48.47%), Query Frame = 1
Query: 424 FIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEA-MNLEYQLNKDPIFGIWKAKAVFDGQEEIIQ-FEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPM 906
F+ +T+KPIYKPG V+ R +++ D+N+ + ++ P++ R+AQW + SI+ ++L + + GI+ A D E + FEIK+YVLP+F V V P+ I D+ ++ K+C KY+ GKP+
Sbjct: 104 FVHIVQTEKPIYKPGQTVQFRIVSM-----------------------------------DTNFIPVNRMD-PNSNRIAQWMDKSIDGGILDLSHSTIPEAAQGIYVITAETDKGETVSHNFEIKEYVLPKFEVTVNLPTVITILDDEVTLKICGKYTYGKPV 230
BLAST of Alpha-2-macroglobulin vs. Planmine SMEST
Match: SMESG000037203.1 (SMESG000037203.1) HSP 1 Score: 3580.8 bits (9284), Expect = 0.000e+0 Identity = 1804/1853 (97.36%), Postives = 1819/1853 (98.17%), Query Frame = 1
Query: 85 FAVKDFYFITSPNLLITETSHHFNVHTSTDIVLTIESIHDPSQIKHQSSKFVIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSNLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSGGCLNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPITKNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYEWNLELEPKNCSCLNSWESFNKSFVILSSRWRSLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYYSVIKNITEWYCANFTDWKLNISSTSPIVKGVLSSNVSSIPVELEFPHQFCYCYEHVRDTKKSFRIVDLNHKLQSLNQKPKDPIKLMLNKGFEVVDEEIFSSLKNIKCG 5643
FAVKDFYFITSPNLLITETSHHFNVHTSTDIVLTIESIHDPSQIKHQSSKFVIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREP+EIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYG KKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSW YGNNWGFIRNYTSNKKGVI+FVIPYIPLAQSLSIQISEVSSNLKPPM ATT YQ LTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSG CLNGACVEPNKCICKQYWTGKGCN+C KAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPITKNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCA+RI DEALLQFGQSSNADLSYLVQ LI LN KI D++ K CIPQKRVKRMI PIYG YDSRKVLSTAGFTAITNARIVNVKCM LA GMPDRSY TF + RTQM++S NSVLKISDAK+KKQIREYFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDSSSKYSSFCSEKKKKKINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYL+SLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADG+LLTAYVLISLNGVDLTNTTINITKAINCATENITNNTPNY+LALTSYLLSLYTPSSSLSQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWELSEEEKKSLSLQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFN+ CNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYEWNLELEPKNCSCLNSWESFNKSFVILSSRWRSLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYYSVIKNITEWYCANFTDWKLNISSTSPIVKGVLS NVSS PVEL+FPHQFCYCYEHVRDTKKSFRIVDLNHKLQSLNQKPKDPIKLMLNK FEVVDEEIFSSLKNIKCG
Sbjct: 18 FAVKDFYFITSPNLLITETSHHFNVHTSTDIVLTIESIHDPSQIKHQSSKFVIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPEEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGTKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWEYGNNWGFIRNYTSNKKGVIDFVIPYIPLAQSLSIQISEVSSNLKPPMTATTSYQLLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSGDCLNGACVEPNKCICKQYWTGKGCNRCIKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPITKNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAVRITDEALLQFGQSSNADLSYLVQMLITLNKKIANDNQRIFYGKPWCIPQKRVKRMILPIYGFGYDSRKVLSTAGFTAITNARIVNVKCMHILAMGMPDRSYYTFESARTQMTQSGNSVLKISDAKLKKQIREYFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDSSSKYSSFCSEKKKKKINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLSSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGQLLTAYVLISLNGVDLTNTTINITKAINCATENITNNTPNYKLALTSYLLSLYTPSSSLSQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWELSEEEKKSLSLQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNRDCNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYEWNLELEPKNCSCLNSWESFNKSFVILSSRWRSLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYYSVIKNITEWYCANFTDWKLNISSTSPIVKGVLSRNVSSFPVELKFPHQFCYCYEHVRDTKKSFRIVDLNHKLQSLNQKPKDPIKLMLNKRFEVVDEEIFSSLKNIKCG 1870
BLAST of Alpha-2-macroglobulin vs. Planmine SMEST
Match: SMESG000037144.1 (SMESG000037144.1) HSP 1 Score: 2527.67 bits (6550), Expect = 0.000e+0 Identity = 1278/1457 (87.71%), Postives = 1340/1457 (91.97%), Query Frame = 1
Query: 88 AVKDFYFITSPNLLITETSHHFNVHTSTDIVLTIESIHDPSQIKHQSSKFVIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLDKNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSNLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSGGCLNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPITKNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKITLDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLAR---GMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINVIGLKSFTLCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXINIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTE 4449
A +FYFIT+PN LITET H FNVHTSTDIVLTIESIHDPSQIK+QSSKF+IKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEI+LDK NKF+S SK +F LIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPS ILAKDESISFKVCSKYSTGK M AKI+G+FC +++Y +KKCVAFKSDL+KQNC++IKLES + + +SF Y AKSQINVTVTENGTSSSVSKAVEGPGI YNSF IKFD RPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGF RNYTSNKKGVI+FVIPYIPLAQSLSI+ISEVS+++KPPM ++YQSL NFQS+DGHYLQFMPI KFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSG CLNGACVEPNKCICKQYWTGKGCN+CTKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPITKNETEIRKFEKQTIFER+ISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKE+GCA+RIADEALLQFGQSSNADLSYLVQKLIKLN KI D++ K CIPQK VKRMI PIYG YDSRKVLSTAGFTAITNARIVN+ C + R + + A+F E + + ++KKQIR++FPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEI+ KI VD+NEINVIGLK+FTLCQCKNDEKTVYFNLKPKIIG LN+TI +SSKYS+FCS K INIAMSDSVRRQILVEPPGVPTETTFSELICNKN+ L + VF+IPS+IVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYL + I NPAL AKAK YIIDGY+RQLTYKHDDGSFSAFGK DKFGSTWLTAFVLKSF ASKYVNINLSTLKEATKYLEK+QRSDGCFEEKGKLFSSTMAGGIKRNDK DG+LLTAYVLISLNGVDLTNTTINITKAINCATENITNNTPNYRLALT+Y LSLY S +L+Q V AQL QSVSFGRYKYWPFDKESSDE KSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNL VSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVK +
Sbjct: 19 AKNEFYFITTPNQLITETLHRFNVHTSTDIVLTIESIHDPSQIKNQSSKFIIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIILDKTNKFVS--SKTRKHFRFFHLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSGILAKDESISFKVCSKYSTGKLMTAKINGIFC-IFAYKRKKCVAFKSDLLKQNCSRIKLESNTLNL--TSFAYFTAKSQINVTVTENGTSSSVSKAVEGPGIMYNSFIIKFDSRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFSRNYTSNKKGVIDFVIPYIPLAQSLSIRISEVSNDIKPPMNPASLYQSLANFQSRDGHYLQFMPIKQKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSGDCLNGACVEPNKCICKQYWTGKGCNRCTKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPITKNETEIRKFEKQTIFERMISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKEDGCAVRIADEALLQFGQSSNADLSYLVQKLIKLNKKIANDNQRIFYGKPWCIPQKHVKRMILPIYGFGYDSRKVLSTAGFTAITNARIVNINCHFPIWRFNGPLARVAGASFEKESSSLK-----------TELKKQIRDFFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEIHAKIEVDSNEINVIGLKAFTLCQCKNDEKTVYFNLKPKIIGNLNITIHTSSKYSTFCSNKNLTNINIAMSDSVRRQILVEPPGVPTETTFSELICNKNQTLRLKKVFSIPSKIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLEGMKIDNPALEAKAKRYIIDGYSRQLTYKHDDGSFSAFGKKDKFGSTWLTAFVLKSFQAASKYVNINLSTLKEATKYLEKIQRSDGCFEEKGKLFSSTMAGGIKRNDKTDGQLLTAYVLISLNGVDLTNTTINITKAINCATENITNNTPNYRLALTAYSLSLYNFSLNLTQQVIAQLDKQSVSFGRYKYWPFDKESSDEHKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLNVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKNQ 1459
BLAST of Alpha-2-macroglobulin vs. Planmine SMEST
Match: SMESG000037149.1 (SMESG000037149.1) HSP 1 Score: 1513.05 bits (3916), Expect = 0.000e+0 Identity = 778/889 (87.51%), Postives = 826/889 (92.91%), Query Frame = 1
Query: 2980 LCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYEWNLELEPKNCSCLNSWESFNKSFVILSSRWRSLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYYSVIKNITEWYCANFTDWKLNISSTSPIVKGVLSSNVSSIPVELEFPHQFCYCYEHVRDTKKSFRIVDLNHKLQSLNQKPKDPIKLMLNKGFEVVDEEIFSSLKNIKCG 5643
+C C N+EKTVYFN++PK+IGKLN+TI SSS+YS+FCS++ + K N+ M DSVRRQILVEPPGVP ETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLV MPYGCGEQNMVAVVPSIYVLDYL+SLNI+NPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSF DASKYV+I S L +A YLE Q+ DGCFEEKGKL SSTMAG +K+ND +D LLTAYVLIS+ + LT + N+ +A+ C +N+ + +RLALT+Y +SLY SS+L+++V QL+ QSVS GRYKYWPFDKESSD ++SNSADIETTGYAILALINHQDLS SMK VYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWELSEEEKKSLSLQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFN+ CNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYE NLELEPKNCSCLNSWESFNK+FVILSSRWRSLNEKLTLSNDSIMFPM TLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYY VIKNITEWYCAN+T W LN+SSTSPIVKGVLSSNVSSIPVELEFPHQFCYCYEHVRD K+SFRIVDLNHKLQSLNQKPKDPIKL+LN F++V+ EIFSSLK IKC
Sbjct: 1 MCLCSNEEKTVYFNIQPKLIGKLNITIHSSSRYSTFCSKENENKTKNVEMFDSVRRQILVEPPGVPMETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVRMPYGCGEQNMVAVVPSIYVLDYLSSLNIENPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFTDASKYVDIQKSILIKAMNYLENSQKPDGCFEEKGKLLSSTMAGNVKQNDNSDSSLLTAYVLISIKRMKLTKSNFNVFQALKCVAKNLEKESSTFRLALTAYAMSLYDGSSNLTRSVMNQLVKQSVSIGRYKYWPFDKESSDIKRSNSADIETTGYAILALINHQDLSESMKIVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWELSEEEKKSLSLQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNRDCNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYELNLELEPKNCSCLNSWESFNKNFVILSSRWRSLNEKLTLSNDSIMFPMATLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYYFVIKNITEWYCANYTYWNLNVSSTSPIVKGVLSSNVSSIPVELEFPHQFCYCYEHVRDMKRSFRIVDLNHKLQSLNQKPKDPIKLVLNNEFQIVEGEIFSSLKYIKCA 889
BLAST of Alpha-2-macroglobulin vs. Planmine SMEST
Match: SMESG000037155.1 (SMESG000037155.1) HSP 1 Score: 1321.61 bits (3419), Expect = 0.000e+0 Identity = 686/785 (87.39%), Postives = 728/785 (92.74%), Query Frame = 1
Query: 2980 LCQCKNDEKTVYFNLKPKIIGKLNVTIDXXXXXXXXXXXXXXXXI-NIAMSDSVRRQILVEPPGVPTETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVVMPYGCGEQNMVAVVPSIYVLDYLNSLNIKNPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFHDASKYVNINLSTLKEATKYLEKMQRSDGCFEEKGKLFSSTMAGGIKRNDKADGKLLTAYVLISLNGVDLXXXXXXXXXXXXXXXENITNNTPNYRXXXXXXXXXXXXXXXXXXQNVFAQLLDQSVSFGRYKYWPFDKESSDEQKSNSADIETTGYAILALINHQDLSVSMKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVSQNVNLKVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWXXXXXXXXXXXXQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKIVDYYNPEIYSEKSYSMFNKACNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYEWNLELEPKNCSCLNSWESFNKSFVILSSRWRSLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIANNFYLMANRLIPDFANKTAKNDSCPKCILRNNYYSVIK 5331
+C C N+EKTVYFN++PK+IGKLN+TI SSS+YS+FCS++ + K N+ M DSVRRQILVEPPGVP ETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLV MPYGCGEQNMVAVVPSIYVLDYL+SLNI+NPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSF DASKYV+I S L +A YLE Q+ DGCFEEKGKLFSSTMAG +K+ND D LLTAY+LIS+ + LT + N+ +A+ C +N+ + +RLALT+Y +SLY SS+L+++V QL+ QSVS GRYKYWPFDKESSDE KSNSADIETTGYAILALINHQDLS S+KTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGV QNVNL VSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWELSEEEKKSLSLQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKI+DYYNPEIYSE SYSMFN+ CNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYE NLELEPKNCSCLNSWESFNKSFVILSSRW+SLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIAN FYLMANRLIPDFANKTAKNDSCPKCILRNNYYS+ K
Sbjct: 1 MCLCSNEEKTVYFNIQPKLIGKLNITIHSSSRYSTFCSKENENKTKNVEMFDSVRRQILVEPPGVPMETTFSELICNKNEQLGILSVFAIPSEIVSGSLRSYLTFNGDVMGKTFDNLDKLVRMPYGCGEQNMVAVVPSIYVLDYLSSLNIENPALSAKAKSYIIDGYTRQLTYKHDDGSFSAFGKTDKFGSTWLTAFVLKSFTDASKYVDIQKSILIKAMNYLENSQKPDGCFEEKGKLFSSTMAGNVKQNDNTDSSLLTAYILISIKRMKLTKSNFNVFQALKCVAKNLEKESSTFRLALTAYAMSLYDGSSNLTRSVMNQLVKQSVSIGRYKYWPFDKESSDEHKSNSADIETTGYAILALINHQDLSESLKTVYWLSNQRNSLGGYYSTQDTVVALNALAVFAKTIGVHQNVNLNVSVVLNRNKTYNGLITLDNRLVSQTFNLGTENISYVSMKTNGSGCASIQLSSLYNIHRLYAANDTFKLSVKTEIDSVKQCNIRNIKVCFAYLRRDKNSNMILVIINYPSGWELSEEEKKSLSLQISTPLNRIDFQKTRMELYYDAFSYNDAGKDSCFRFKIEEKTKISNLQPAVVKILDYYNPEIYSEMSYSMFNRDCNKSDEKEPGRPEVLLCPICVNDSINSTLKFIGTICNAWFNGFVTANSNASQRRTVFVAEQSMFYELNLELEPKNCSCLNSWESFNKSFVILSSRWKSLNEKLTLSNDSIMFPMTTLYSAYQFDLQAQCNIANYFYLMANRLIPDFANKTAKNDSCPKCILRNNYYSLSK 785
BLAST of Alpha-2-macroglobulin vs. Planmine SMEST
Match: SMESG000037154.1 (SMESG000037154.1) HSP 1 Score: 940.643 bits (2430), Expect = 0.000e+0 Identity = 472/967 (48.81%), Postives = 635/967 (65.67%), Query Frame = 1
Query: 88 AVKDFYFITSPNLLITETSHHFNVHTSTDIVLTIESIHDPSQIKHQSSKFVIKKGFQTIPYYVPFVGSHLGSKIRLNILGDVCQSGMKCELTKNITGNFINETREIGLIDKNFIIFAETDKPIYKPGDKVRLRFMAIYPTNLMREPDEIGKQVEIVLD--KNNKFISKPSKNDNDSNYFDLIIIETPDNVRVAQWKNVSINEAMNLEYQLNKDPIFGIWKAKAVFDGQEEIIQFEIKKYVLPRFIVEVIPPSEILAKDESISFKVCSKYSTGKPMNAKISGMFCMLYSYGKKKCVAFKSDLMKQNCNKIKLESKIVFILASSFRYSKAKSQINVTVTENGTSSSVSKAVEGPGIKYNSFNIKFDGRPYYKPGLPYFGRINLVRHNKKPAKMVNISISIQSWAYGNNWGFIRNYTSNKKGVIEFVIPYIPLAQSLSIQISEVSSNLKPPMAATTVYQSLTNFQSKDGHYLQFMPINDKFKCPGKLNLTLLTNYDIASTSIYVQIFSRGQIVKMIEYPSVPVKCYPRDDFFGHYQCVNGTTLTCLSGWKGGNCREPICSGGCLNGACVEPNKCICKQYWTGKGCNQCTKAKYCKNGYCVNGGDCECYPGWMNFDCSKKGPIKHLYRNVTTVKPIT------KNETEIRKFEKQTIFERIISIPLMKNMGPDVHVIAYFINTDNEVVADALEIASGLNNCSQLGGGKFKFNKKTAMPKSKVVLTVDPGVKENGCAIRIADEALLQFGQSSNADLSYLVQKLIKLNHKIT--LDSRIGLLRKHKCIPQKRVKRMIQPIYGLEYDSRKVLSTAGFTAITNARIVNVKCMQYLARGMPDRSYATFSNERTQMSKSRNSVLKISDAKMKKQIREYFPESWVFSYFNKTTKHLNLTVPDSMTTWRADSYCMTEKGLWIAPTQKLKVILPFYSNIYVPYSMKLNEVLQLPVKVFNYAGTCFEINVKINVDANEINV 2958
A KDFYFIT P +L+ E+SH ++H+ST++++ I + H S I+H+S F IKKG IPY +PF + I L LGD+C +KC+ T+N+ G + NET + +I++NFIIF+ETDKPIYKPG+++ LRFM IYPTNL +P + Q IV D +N K I N + F+LI+IE RVAQW+N++I AMNL YQL+KDP GIWKAKA++ GQEE+++F + KYVLPRFI+ + PP++I+ KD SI +++C++YST K M A+I FC++Y Y +KC L C I++ +K VF + +R+ + +S+INVTVTENGTSS++SK+++G GI + I F+ +PY+K GLPYFG + L+RH+ KP + I IS+ WGF NY+S++ GV+ F+IP + +IQI+E S ++ + +++ ++S L+ +PI +K KCPGKLNLT+LTNYDI+ST++YVQI SRGQIVK IEYP VP+KCY RDD GHY+CV+ TL CL GWKG NC EPICS GC+NGAC +KC+CK W G+ C++C K YCKNG C G DC C PGW +DCS KG Y N P++ K K+E ++IFERI+SIPL+KNMGPD+HVI YFIN EV+ D +E+ GL +CS +GGG+F F+K TA+PKSKV+L +DPGV+ CA+RIAD L QFGQSSN DLSYLVQKLI +NHKI + L HK V P G +DS + +GF A+TNARIVN KC +G+ F + ++ S + V K++ +REYFP SW+F YFN T K L LTVPDS+TTW+A S+CMTE G+WIA Q L V F++ IYVPYSMKLNE L LP+K+FNY C+EI +I+ + NEI V
Sbjct: 19 AAKDFYFITMPKVLLVESSHKISLHSSTNLLVKIVTFHTKSPIRHESETFYIKKGISLIPYQLPFYDKTNEASISLRFLGDICDELIKCDPTQNVYGKYFNETHILNIINQNFIIFSETDKPIYKPGERIMLRFMPIYPTNLKTDPPMLFNQRVIVGDGKENYKLI-----NSDIGKTFELIVIENSIEARVAQWRNLTIENAMNLNYQLSKDPAIGIWKAKAIYRGQEEVMEFRVMKYVLPRFIINITPPNQIMYKDSSIKYEICAQYSTKKKMIARIQTTFCIIYYYVTEKCQVTNLILKDTYCATIEVSTKSVFPAFNKYRFIRTRSRINVTVTENGTSSTISKSLDGNGIMDTEYKIVFNSKPYFKFGLPYFGNVKLIRHDNKPQRNTKIKISVDFPKRYVKWGFESNYSSDENGVVRFIIPSVISNSLFTIQITEASDVIENVITTPIFSRNIDPWESISEQVLEILPIENKIKCPGKLNLTVLTNYDISSTNLYVQILSRGQIVKAIEYPPVPIKCYSRDDDLGHYKCVDDKTLQCLPGWKGNNCIEPICSRGCINGACTSHDKCVCKMGWVGETCDKCIKPAYCKNGNCNRGNDCVCLPGWTKYDCSIKG---FRYNNEPLYVPLSKYTEPPKKPAVTNKYENKSIFERILSIPLLKNMGPDIHVIVYFINAQLEVIVDTVELLEGLESCSNVGGGEFIFDKNTALPKSKVILKIDPGVQNASCAVRIADIVLFQFGQSSNVDLSYLVQKLIYINHKIINYKNDVTNCLSDHKISQYSFV-----PETG--FDSDFSIKNSGFKAVTNARIVNKKCK---FKGL-------FPSSTNKIEIS-DFVFGEEKMKLQTHVREYFPGSWIFKYFNHTPKQLFLTVPDSLTTWQARSFCMTENGIWIAVPQILTVHKLFFTEIYVPYSMKLNERLHLPIKLFNYVDICYEIRSEISTNTNEIYV 959 The following BLAST results are available for this feature:
BLAST of Alpha-2-macroglobulin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Alpha-2-macroglobulin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Alpha-2-macroglobulin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004348 ID=SMED30004348|Name=Alpha-2-macroglobulin|organism=Schmidtea mediterranea sexual|type=transcript|length=5775bpback to top protein sequence of SMED30004348-orf-1 >SMED30004348-orf-1 ID=SMED30004348-orf-1|Name=SMED30004348-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1871bp MNSVVLITIIISIVTKVFAVKDFYFITSPNLLITETSHHFNVHTSTDIVLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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