Slc16a-7

Overview
NameSlc16a-7
Smed IDSMED30004339
Length (bp)1662
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Slc16a-7 (SMED30004339) t-SNE clustered cells

Violin plots show distribution of expression levels for Slc16a-7 (SMED30004339) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Slc16a-7 (SMED30004339) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Slc16a-7 (SMED30004339) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004339

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30004339SMESG000023798.1 SMESG000016446.1 dd_Smed_v4_13379_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30004339SMESG000023798.1 SMESG000016446.1 dd_Smed_v4_13379_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30004339SMESG000023798.1 SMESG000016446.1 dd_Smed_v4_13379_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30004339SMESG000023798.1 SMESG000016446.1 dd_Smed_v4_13379_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Slc16a-7 vs. TrEMBL
Match: A0A0H3YF94 (Slc16a-7 OS=Schmidtea mediterranea OX=79327 GN=slc16a-7 PE=2 SV=1)

HSP 1 Score: 408.683 bits (1049), Expect = 1.392e-134
Identity = 235/439 (53.53%), Postives = 323/439 (73.58%), Query Frame = 1 
Query:  337 KTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNN-TESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGXXXXXXXXXXXXXXXGFQIN-IKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYXXXXXXXXXXXXXFGDFFRGWLVXXXXXXXXXXXXXXXXYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE 1647
            + +++W+ LVIIGT+LG+II G++PHSFGT IA+FKS NN T +PSFY  SQF  N+LPA SSA PCILLPLFV+IIQ+  + YQ++SAL F+++AISCGLSSLV++W + F TYS++YSIG+SLFL VV+II AEYFP  HRFHVLATSL+QIGYP AAL GNP+LA L+  YGWQ + K  A  +++ G I++ +MK +  K          +N   K E   S + + S   ++ +    ++  WL+ K++NY+CWY LRTN+VTYLVKST FGL  SATLMS+YA +E+ +A+ AS+ G+ F+G+L+HIH+ SS++LA+LT +YT+ +TSY+SAV++M+ IG ++Q SL+FGYPA+ EV  +  D+K GW+LTNV+AGFGI  SPL SG LMD+  +K+L YAQ  GW LQ  L+  TL+ +K  + +     IE
Sbjct:   19 QEMTVWNWLVIIGTSLGSIISGIIPHSFGTFIAHFKSDNNKTNNPSFYFVSQFEWNWLPALSSAVPCILLPLFVLIIQKINRSYQMLSALGFVIIAISCGLSSLVSLWYVFFFTYSLLYSIGSSLFLAVVVIILAEYFPVNHRFHVLATSLFQIGYPAAALIGNPLLAILIINYGWQVALKFTAFFEVITGVIIALLMKPVRDKSFCKHSEGSSENCPIKAELFSSTQRTLSATSMHYLAYSLELWTWLIAKFLNYLCWYGLRTNLVTYLVKSTNFGLEKSATLMSIYAGAEVIVAMSASIIGNGFKGYLMHIHVFSSMALAVLTGLYTIAITSYLSAVIVMICIGAAVQSSLNFGYPASVEVVKAKFDMKTGWILTNVAAGFGIFISPLFSGILMDRFNVKALFYAQSCGWALQAFLYLITLLIIKSNQNKLPTDYIE 457
[Close]
BLAST of Slc16a-7 vs. TrEMBL
Match: A0A267GQ83 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029081g1 PE=4 SV=1)

HSP 1 Score: 195.667 bits (496), Expect = 2.254e-52
Identity = 138/396 (34.85%), Postives = 233/396 (58.84%), Query Frame = 1 
Query:  346 SIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKG-LSYIKTTEVDNGXXXXXXXXXXXXXXXGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYXXXXXXXXXXXXXFGDFFRGWLVXXXXXXXXXXXXXXXXYTVVLT-SYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKV 1527
            ++WH L+ + T+L  ++E  VPHSFGT +   + + N+        S   LN++  ++S+ P I+LP+   ++      +++++A++ L +  S  +++  ++ P+ FL+YS++Y + +    GVV+   AEYFP  H +HVLATSL+QIGYP AA+  NP  A L+ ++GW+ + +I+A I  ++G+ L+C++K + +KG L +   ++    +     T ++          +    Q+  W +GK +NY CW+ALRTN+V Y+ +     L+ SA LM  Y   EI++ L A L GD   G L+H+H++++  +A+LT ++  V + SY SA+ +   IG  +QLSL+F YPAAAEV  +  D+     +T  + G GI+ SPLI G L D V
Sbjct:   58 TLWHALICLATSLLILVEASVPHSFGTYLVAIREETNS--------SLLLLNWVTVATSSVPYIMLPVSTALLAHCVNWWRLVAAISCLGMGGSLLITAGQSLVPLFFLSYSLVYGVCSCALHGVVLSCLAEYFPVGHPWHVLATSLFQIGYPCAAVIFNPSAASLINRFGWRVAMRIMAAIVAIVGTCLACLLKPVGAKGLLDFSFKSDCTAAEQPAPVTAASAVCRCCTPSQMLRRPQISIWFVGKLLNYACWFALRTNLVLYM-RLAGHSLAESAGLMIAYGLGEITVCLAACLLGDRIAGRLLHVHMVAACGMALLTGVWWFVASASYASALTVGCLIGAFVQLSLTFTYPAAAEV--TRLDLVTAGSVTLGAGGLGILVSPLIGGLLFDLV 442
[Close]
BLAST of Slc16a-7 vs. TrEMBL
Match: A0A1I8J8I8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.644e-47
Identity = 138/396 (34.85%), Postives = 232/396 (58.59%), Query Frame = 1 
Query:  346 SIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKG-LSYIKTTEVDNGXXXXXXXXXXXXXXXGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYXXXXXXXXXXXXXFGDFFRGWLVXXXXXXXXXXXXXXXX-YTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKV 1527
            ++WH L+ + T+L  ++E  VPHSFGT +   + + N+        S   LN++  ++S+ P I+LP+   ++      +++++A++ L +  S  +++  ++ P+ FL+YS++Y + +    GVV+   AEYFP  H +HVLATSL+QIGYP AA+  NP  A L+ ++GW+ + +I+A I  ++G+ L+C++K + +KG L +   ++    +     T ++          +    Q+  W +GK +NY CW+ALRTN+V Y+ +     L+ SA LM  Y   EI++ L A L GD   G L+H+H++++  +A+LT + + V   SY SA+ +   IG  +QLSL+F YPAAAEV  +  D+     +T  + G GI+ SPLI G L D V
Sbjct:  616 TLWHALICLATSLLILVEASVPHSFGTYLVAIREETNS--------SLLLLNWVTVATSSVPYIMLPVSTALLAHCVNWWRLVAAISCLGMGGSLLITAGQSLVPLFFLSYSLVYGVCSCALHGVVLSCLAEYFPVGHPWHVLATSLFQIGYPCAAVIFNPSAASLINRFGWRVAMRIMAAIVAIVGTCLACLLKPVGAKGLLDFSFKSDCTAAEQPAPVTAASAVCRCCTPSQMLRRPQISIWFVGKLLNYACWFALRTNLVLYM-RLAGHSLAESAGLMIAYGLGEITVCLAACLLGDRIAGRLLHVHMVAACGMALLTGVWWFVASASYASALTVGCLIGAFVQLSLTFTYPAAAEV--TRLDLVTAGSVTLGAGGLGILVSPLIGGLLFDLV 1000
[Close]
BLAST of Slc16a-7 vs. TrEMBL
Match: A0A1I8HA83 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 9.895e-35
Identity = 87/266 (32.71%), Postives = 156/266 (58.65%), Query Frame = 1 
Query:  346 SIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKG-LSYIKTTEVDNGXXXXXXXXXXXXXXXGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYL 1140
            S+WH L+   T+L  ++E  VPHSFGT +   + + N+        S   LN++  ++S+ P I+LP+   ++      +++++A++ L +  S  +++  ++ P+ FL+YS++Y + +    GVV+   AEYFP  H +HVLATSL+QIGYP AA+  NP  A L+ ++GW+ + +I+A I  ++G+ L+C++K + +KG L +   ++    +     T ++          +    Q+  W +GK +NY CW+ALRTN+V Y+
Sbjct:  589 SLWHALICFATSLLILVEASVPHSFGTYLVAIREETNS--------SLLLLNWVTVATSSVPYIMLPVATALLAHCVNWWRLVAAISCLGMGGSLLITAGQSLVPLFFLSYSLVYGVCSCALHGVVLSCLAEYFPVGHPWHVLATSLFQIGYPCAAVIFNPSAASLINRFGWRVAMRIMAAIVAIVGTCLACLLKPVGAKGLLDFSFKSDCTAAEQPAPVTAASAVCRCCTPSQMLRRPQISIWFVGKLLNYACWFALRTNLVLYM 846
[Close]
BLAST of Slc16a-7 vs. TrEMBL
Match: A0A1I8IEB8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 1.916e-27
Identity = 72/215 (33.49%), Postives = 132/215 (61.40%), Query Frame = 1 
Query:  316 YEMSELFKT---LSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGL 951
            ++  +L K     ++WH L+ + T+L  ++E  VPHSFGT +   + + N+        S   LN++  ++S+ P I+LP+   ++      +++++A++ L +  S  +++  ++ P+ FL+YS++Y + +    GVV+   AEYFP  H +HVLATSL+QIGYP AA+  NP  A L+ ++GW+ + +I+A I  + G+ L+C++K + +KGL
Sbjct:  569 HQQQQLEKDSAKCTLWHALICLATSLLILVEASVPHSFGTYLVAIREETNS--------SLLLLNWVTVATSSVPYIMLPVSTALLAHCVNWWRLVAAISCLGMGGSLLITAGQSLVPLFFLSYSLVYGVCSCALHGVVLSCLAEYFPVGHPWHVLATSLFQIGYPCAAVIFNPSAASLINRFGWRVAMRIMAAIVAIAGTCLACLLKPVAAKGL 775
[Close]
BLAST of Slc16a-7 vs. Ensembl Nematostella
Match: EDO46709 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPB5])

HSP 1 Score: 51.6026 bits (122), Expect = 4.415e-7
Identity = 29/90 (32.22%), Postives = 48/90 (53.33%), Query Frame = 1 
Query:  634 LSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILL 903
            L+S V   P ++LTY +++ +G+SL     I+I  EYF K      L   +   G  + +L   P++ YLL   GWQN  ++++   +LL
Sbjct:   91 LTSFVQEPPKMYLTYGLLFGVGSSLSFVSSIVILGEYFQKR---LALVNGIATSGSGVGSLVTGPVMNYLLANIGWQNCMRVLSGFAMLL 177
[Close]
BLAST of Slc16a-7 vs. Ensembl Nematostella
Match: EDO46666 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPD0])

HSP 1 Score: 51.2174 bits (121), Expect = 8.019e-7
Identity = 30/90 (33.33%), Postives = 50/90 (55.56%), Query Frame = 1 
Query:  634 LSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILL 903
            L+S V     +++TY +++ +G+ L     II+  +YF K  RF  LA  +   G  +  L   P + +LL+ +GWQNS +I+A I  +L
Sbjct:  108 LTSFVQNPGKMYVTYGLLWGVGSGLSFTPSIIVLGQYFRK--RFP-LANGIASSGSGIGGLLAAPFIQFLLSNFGWQNSMRILACIANIL 194
[Close]
BLAST of Slc16a-7 vs. Ensembl Nematostella
Match: EDO38356 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SD36])

HSP 1 Score: 50.0618 bits (118), Expect = 2.468e-6
Identity = 27/74 (36.49%), Postives = 39/74 (52.70%), Query Frame = 1 
Query:  664 LFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIA 885
            +F +YSV+Y +G+ LF    +   AEYF K      LA      G  +  L   P+L  LL  YGW+ +F+I+A
Sbjct:  113 IFFSYSVLYGLGSCLFYMSCVYAVAEYFEKRRG---LALGFLTSGISVGVLAQGPVLQVLLDAYGWRGTFRIMA 183

HSP 2 Score: 48.521 bits (114), Expect = 6.858e-6
Identity = 27/74 (36.49%), Postives = 39/74 (52.70%), Query Frame = 1 
Query:  664 LFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIA 885
            +F +YSV+Y +G+ LF    +   AEYF K      LA      G  +  L   P+L  LL  YGW+ +F+I+A
Sbjct:  518 IFFSYSVLYGLGSCLFYMSCVYAVAEYFEKRRG---LALGFLTSGISVGVLAQGPVLQVLLDAYGWRITFRIMA 588
[Close]
BLAST of Slc16a-7 vs. Planmine SMEST
Match: SMESG000023798.1 (SMESG000023798.1)

HSP 1 Score: 762.681 bits (1968), Expect = 0.000e+0
Identity = 441/442 (99.77%), Postives = 442/442 (100.00%), Query Frame = 1 
Query:  322 MSELFKTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGXXXXXXXXXXXXXXXGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYXXXXXXXXXXXXXFGDFFRGWLVXXXXXXXXXXXXXXXXYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE 1647
            MSELFKTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQIISALAFLLLAISCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVII+AAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGKTKEESTKSNESSHSGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYAASEISIALIASLFGDFFRGWLVHIHLLSSISLAILTAIYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE
Sbjct:    1 MSELFKTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQIISALAFLLLAISCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIVAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGKTKEESTKSNESSHSGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYAASEISIALIASLFGDFFRGWLVHIHLLSSISLAILTAIYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE 442
[Close]
BLAST of Slc16a-7 vs. Planmine SMEST
Match: SMESG000016446.1 (SMESG000016446.1)

HSP 1 Score: 762.681 bits (1968), Expect = 0.000e+0
Identity = 441/442 (99.77%), Postives = 442/442 (100.00%), Query Frame = 1 
Query:  322 MSELFKTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGXXXXXXXXXXXXXXXGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYXXXXXXXXXXXXXFGDFFRGWLVXXXXXXXXXXXXXXXXYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE 1647
            MSELFKTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQIISALAFLLLAISCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVII+AAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGKTKEESTKSNESSHSGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYAASEISIALIASLFGDFFRGWLVHIHLLSSISLAILTAIYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE
Sbjct:    1 MSELFKTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQIISALAFLLLAISCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIVAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGKTKEESTKSNESSHSGFQINIKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYAASEISIALIASLFGDFFRGWLVHIHLLSSISLAILTAIYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE 442
[Close]
BLAST of Slc16a-7 vs. Planmine SMEST
Match: SMESG000016424.1 (SMESG000016424.1)

HSP 1 Score: 409.068 bits (1050), Expect = 2.536e-138
Identity = 235/439 (53.53%), Postives = 324/439 (73.80%), Query Frame = 1 
Query:  337 KTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNN-TESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGXXXXXXXXXXXXXXXGFQIN-IKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYXXXXXXXXXXXXXFGDFFRGWLVXXXXXXXXXXXXXXXXYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE 1647
            + +++W+ LVIIGT+LG+II G++PHSFGT IA+FKS NN T +PSFY  SQF  N+LP+ SSA PCILLPLFV+IIQ+  + YQ++SAL F+++AISCGLSSLV++W + F TYS++YSIG+SLFL VV+II AEYFP  HRFHVLATSL+QIGYP AAL GNP+LA L+  YGWQ + K  A  +++ G I++ +MK +  K          +N   K E + S + + S   ++ +    ++  WL+ K++NY+CWY LRTN+VTYLVKST FGL  SATLMS+YA +E+ +A+ AS+ G+ F+G+L+HIH+ SS++LA+LT +YT+ +TSY+SAV+LM+ IG ++Q SL+FGYPA+ EV  +  D+K GW+LTNV+AGFGI  SPL SG LMD+  +K+L YAQ  GW LQ  L+  TL+ +K  + +     IE
Sbjct:   19 QEMTVWNWLVIIGTSLGSIISGIIPHSFGTFIAHFKSDNNKTNNPSFYFVSQFEWNWLPSLSSAVPCILLPLFVLIIQKINRSYQMLSALGFVIIAISCGLSSLVSLWYVFFFTYSLLYSIGSSLFLAVVVIILAEYFPVNHRFHVLATSLFQIGYPAAALIGNPLLAILIINYGWQVALKFTAFFEVITGVIIALLMKPVRDKSFCKHSEGSSENCPIKAELSSSTQRTLSATSMHYLAYSLELWTWLIAKFLNYLCWYGLRTNLVTYLVKSTNFGLEKSATLMSIYAGAEVIVAMSASIIGNGFKGYLMHIHVFSSMALAVLTGLYTIAITSYLSAVILMICIGAAVQSSLNFGYPASVEVVKAKFDMKTGWILTNVAAGFGIFISPLFSGILMDRFNVKALFYAQSCGWALQAFLYLITLLIIKSNQNKLPTDYIE 457
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BLAST of Slc16a-7 vs. Planmine SMEST
Match: SMESG000023800.1 (SMESG000023800.1)

HSP 1 Score: 409.068 bits (1050), Expect = 2.889e-138
Identity = 235/439 (53.53%), Postives = 324/439 (73.80%), Query Frame = 1 
Query:  337 KTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNN-TESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSCMMKSIDSKGLSYIKTTEVDNGXXXXXXXXXXXXXXXGFQIN-IKSDYQVIPWLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYXXXXXXXXXXXXXFGDFFRGWLVXXXXXXXXXXXXXXXXYTVVLTSYISAVLLMMAIGVSIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMDKVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE 1647
            + +++W+ LVIIGT+LG+II G++PHSFGT IA+FKS NN T +PSFY  SQF  N+LPA SSA PCILLPLFV+IIQ+  + YQ++SAL F+++AISCGLSSLV++W + F TYS++YSIG+SLFL VV+II AEYFP  H+FHVLATSL+QIGYP AAL GNP+LA L+  YGWQ + K  A  +++ G I++ +MK +  K          +N   K E + S + + S   ++ +    ++  WL+ K++NY+CWY LRTN+VTYLVKST FGL  SATLMS+YA +E+ +A+ AS+ G+ F+G+L+HIH+ SS++LA+LT +YT+ +TSY+SAV+LM+ IG ++Q SL+FGYPA+ EV  +  D+K GW+LTNV+AGFGI  SPL SG LMD+  +K+L YAQ  GW LQ  L+  TL+ +K  + +     IE
Sbjct:   19 QEMTVWNWLVIIGTSLGSIISGIIPHSFGTFIAHFKSDNNKTNNPSFYFVSQFEWNWLPALSSAVPCILLPLFVLIIQKINRSYQMLSALGFVIIAISCGLSSLVSLWYVFFFTYSLLYSIGSSLFLAVVVIILAEYFPVNHKFHVLATSLFQIGYPAAALIGNPLLAILIINYGWQVALKFTAFFEVITGVIIALLMKPVRDKSFCKHSEGSSENCPIKAELSSSTQRTLSATSMHYLAYSLELWTWLIAKFLNYLCWYGLRTNLVTYLVKSTNFGLEKSATLMSIYAGAEVIVAMSASIIGNGFKGYLMHIHVFSSMALAVLTGLYTIAITSYLSAVILMICIGAAVQSSLNFGYPASVEVVKAKFDMKTGWILTNVAAGFGIFISPLFSGILMDRFNVKALFYAQSCGWALQAFLYLITLLIIKSNQNKLPTDYIE 457
[Close]
BLAST of Slc16a-7 vs. Planmine SMEST
Match: SMESG000011418.1 (SMESG000011418.1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.892e-17
Identity = 69/218 (31.65%), Postives = 112/218 (51.38%), Query Frame = 1 
Query:  337 KTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPSFYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQXXXXXXXXXXXXXCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHVLATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAV---IQILLGSILSCMMKSIDSKGLSYIKTTEVDN 981
            K LSI+   +++   +  I  G    +FG  I+ F+S       +F   S   LN++  S SA    L+P     + R  + YQ     A +L+  SC L+S V     LF+T++V++ IG++  L    +I  EYF K HR+HVLATSL   G P  +L   P+  +++  Y W+ +F++IA+   ++I  G IL+ + K        Y K+ E++N
Sbjct:   15 KQLSIF---ILVVATINIIFIGGKRRTFGIFISAFRS-------TFNGTSMVELNWVGDSYSAVAFFLMPFITSFVHRN-RSYQKGMLAASILVFCSCMLTSFVTNAAELFITHTVLHGIGSAFILSTSALIIGEYFDKNHRYHVLATSL-TTGGPYGSLIFGPLYGFMVQHYDWRFTFRVIAILFFVEISAG-ILAYVPKP------DYQKSEEIEN 213
The following BLAST results are available for this feature:
BLAST of Slc16a-7 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YF941.392e-13453.53Slc16a-7 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A267GQ832.254e-5234.85Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8J8I81.644e-4734.85Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HA839.895e-3532.71Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IEB81.916e-2733.49Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Slc16a-7 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO467094.415e-732.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO466668.019e-733.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO383562.468e-636.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Slc16a-7 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Slc16a-7 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023798.10.000e+099.77SMESG000023798.1[more]
SMESG000016446.10.000e+099.77SMESG000016446.1[more]
SMESG000016424.12.536e-13853.53SMESG000016424.1[more]
SMESG000023800.12.889e-13853.53SMESG000023800.1[more]
SMESG000011418.11.892e-1731.65SMESG000011418.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004339 ID=SMED30004339|Name=Slc16a-7|organism=Schmidtea mediterranea sexual|type=transcript|length=1662bp
TTTTAATAGTACAATACTAAACTCAATTTTTTAAAATTAAATTCCTTTAT
ATTCAATTTAAATGATTAAATTCAGAAAATATCGGCAATAAATCCGTATT
ATTAGCCAATTAGAAAAGAGACCTACATGTTTCCATTTCACTAAATAGCA
ATTGTACTGAATATATTCTAAATATCAGTCTAATGAGAAAAAAGGAAATT
ATAAGAGACAAAATTGGGGAAAATACAGTTAAGATCTAAATGTTGGAATT
TTACTAACAAAATCCCTATTATACGACTTGCTCAAGTATAAATATGCATA
TAGAAATTCATTTTATATGAAATGTCCGAGTTATTTAAAACTTTATCAAT
TTGGCACGTTCTTGTCATTATCGGGACTGCGTTGGGCACTATCATCGAAG
GTCTTGTGCCTCACAGTTTTGGAACACTAATAGCCTATTTCAAATCGAAG
AACAATACAGAAAGTCCCTCGTTTTATAGCGCTTCTCAATTTCATTTGAA
CTTCCTACCAGCTTCCTCCAGTGCGTTTCCTTGTATTTTATTACCTCTCT
TCGTTGTGATTATTCAGCGCTATTTCAAATTATATCAAATTATATCAGCC
TTAGCCTTCTTATTACTAGCTATCAGTTGTGGTTTGAGTTCTTTGGTGAA
TATTTGGCCAATTCTTTTCCTTACTTACAGTGTGATTTATTCTATCGGCG
CTTCCTTATTCCTCGGTGTGGTTATCATCATTGCTGCGGAATATTTCCCG
AAAGAACACAGATTCCACGTATTGGCAACCAGCTTATACCAAATCGGGTA
TCCGATGGCTGCCTTATTTGGAAATCCTATCCTCGCATATTTATTGACCA
AATATGGATGGCAAAATTCGTTTAAAATCATTGCCGTCATCCAGATATTA
TTAGGTTCAATTCTATCATGCATGATGAAGTCCATAGATAGTAAAGGACT
GTCATACATCAAAACTACCGAAGTTGATAATGGCAAAACTAAAGAGGAAA
GTACAAAAAGCAATGAATCCAGCCATTCTGGCTTCCAAATTAATATAAAG
TCCGATTATCAAGTCATTCCCTGGCTTTTGGGGAAATACATAAATTATAT
GTGCTGGTATGCACTAAGGACAAATATTGTTACGTATCTCGTAAAGTCTA
CTACCTTTGGTTTGTCTAATTCAGCCACGTTAATGTCGGTGTATGCAGCT
TCCGAAATTTCTATTGCTCTTATAGCCTCTTTGTTTGGTGACTTTTTTCG
AGGCTGGCTGGTACATATTCATTTGTTATCTTCAATTTCTTTGGCAATAT
TAACTGCAATATATACTGTAGTATTGACATCATATATCAGTGCAGTTCTT
TTAATGATGGCCATTGGAGTCTCTATTCAACTGTCACTAAGTTTTGGATA
TCCCGCCGCAGCCGAGGTGTGGGATTCAAATTCCGATATTAAAGTCGGAT
GGGTGTTGACAAATGTTTCGGCTGGATTTGGAATAATCTCCAGTCCATTA
ATTAGTGGGTTTCTGATGGATAAAGTTGGAATGAAATCACTACTCTATGC
CCAATGCATCGGTTGGATACTGCAGACGATTTTGTTTGCAGCAACTTTGA
TATGCATGAAATACAAACGATATCAAACAGAATATCAAAACATTGAGTAA
ATAAGTTTTTGA
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protein sequence of SMED30004339-orf-1

>SMED30004339-orf-1 ID=SMED30004339-orf-1|Name=SMED30004339-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=443bp
MSELFKTLSIWHVLVIIGTALGTIIEGLVPHSFGTLIAYFKSKNNTESPS
FYSASQFHLNFLPASSSAFPCILLPLFVVIIQRYFKLYQIISALAFLLLA
ISCGLSSLVNIWPILFLTYSVIYSIGASLFLGVVIIIAAEYFPKEHRFHV
LATSLYQIGYPMAALFGNPILAYLLTKYGWQNSFKIIAVIQILLGSILSC
MMKSIDSKGLSYIKTTEVDNGKTKEESTKSNESSHSGFQINIKSDYQVIP
WLLGKYINYMCWYALRTNIVTYLVKSTTFGLSNSATLMSVYAASEISIAL
IASLFGDFFRGWLVHIHLLSSISLAILTAIYTVVLTSYISAVLLMMAIGV
SIQLSLSFGYPAAAEVWDSNSDIKVGWVLTNVSAGFGIISSPLISGFLMD
KVGMKSLLYAQCIGWILQTILFAATLICMKYKRYQTEYQNIE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 5..211
e-value: 4.5E-9
score: 37.9
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 245..438
e-value: 2.6E-10
score: 41.9
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 11..415
NoneNo IPR availableCDDcd17353MFS_OFA_likecoord: 89..203
e-value: 5.40603E-8
score: 52.6124
NoneNo IPR availableTMHMMTMhelixcoord: 407..429
NoneNo IPR availableTMHMMTMhelixcoord: 375..397
NoneNo IPR availableTMHMMTMhelixcoord: 154..176
NoneNo IPR availableTMHMMTMhelixcoord: 94..116
NoneNo IPR availableTMHMMTMhelixcoord: 186..205
NoneNo IPR availableTMHMMTMhelixcoord: 252..274
NoneNo IPR availableTMHMMTMhelixcoord: 59..81
NoneNo IPR availableTMHMMTMhelixcoord: 7..29
NoneNo IPR availableTMHMMTMhelixcoord: 120..142
NoneNo IPR availableTMHMMTMhelixcoord: 313..335
NoneNo IPR availableTMHMMTMhelixcoord: 340..362
NoneNo IPR availableTMHMMTMhelixcoord: 284..306
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 34..362
e-value: 6.4E-18
score: 64.8
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 15..436
score: 8.974
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 7..426