Parvo_coat_N domain-containing protein

Overview
NameParvo_coat_N domain-containing protein
Smed IDSMED30004312
Length (bp)4260
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Parvo_coat_N domain-containing protein (SMED30004312) t-SNE clustered cells

Violin plots show distribution of expression levels for Parvo_coat_N domain-containing protein (SMED30004312) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Parvo_coat_N domain-containing protein (SMED30004312) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Parvo_coat_N domain-containing protein (SMED30004312) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004312

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30004312 dd_Smed_v4_91560_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30004312 dd_Smed_v4_28220_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V3ZPR9 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_155882 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 7.746e-15
Identity = 43/99 (43.43%), Postives = 61/99 (61.62%), Query Frame = 2 
Query: 1001 PAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLK 1297
            P  KY GE H+PG+N+ GPGTR DLRLD + KP P   PVN +    + HDI Y +K    R+ +D ++I  LEN+   N + ++K+ R LII + + K
Sbjct:  106 PWAKYSGEKHLPGHNYTGPGTRLDLRLDQNDKPKPGEEPVNRVDAAALKHDILYRNKDLKFRHEADKQMIIELENI--PNPTFKEKMQRALIIKLLKTK 202
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V4AHX3 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_162020 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.268e-14
Identity = 61/182 (33.52%), Postives = 97/182 (53.30%), Query Frame = 2 
Query:  998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQDLRLFMKSHFEQQGNVGFVERDRLNAINATAQLFNVFAITT 1543
             P  KY GE H+PG+N+ GPGTR DLRLD +  P P   PVN +    + HDI Y +K    R+ +D ++I  LEN+   N + ++K+ R LII + + K   + +   +  L+ +  D +L   +V +IK F +N       R  MK++ ++Q N+  V R   + +    +L  + AI T
Sbjct:   28 FPWAKYPGEKHLPGHNYTGPGTRLDLRLDENDIPKPGEEPVNRVDAAALKHDILYRNKDVTFRHQADKQMIDELENI--PNPTFKEKLQRALIIKLMKAKLKLDTLKQQLTALDTKITD-ELE-KEVGVIKNFLQN-----KFRDDMKNYIKEQVNL-LVSRIHDDFLTQEKKLSKLEAIVT 199
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BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V4AAM0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_172863 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 2.106e-14
Identity = 50/145 (34.48%), Postives = 79/145 (54.48%), Query Frame = 2 
Query: 1001 PAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNF----EIMAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQD 1423
            P  KY GE H+PG+N+ GPGTR DLRLD +  P P   PVN +    + HDI Y +K    R+ +D ++I  LEN+   N + ++K+ R LII + + K         +  LI  L+++   +K++      ++R  +N  V +D
Sbjct:   29 PWAKYPGEKHLPGHNYTGPGTRLDLRLDENDNPKPGEEPVNRVDAAALKHDILYRNKDITFRHQADKQMIDELENI--PNPTFKEKLQRALIIKLLKAKVKLVNKTSQLQNLISKLDDKITKVKID------VQRLIDNPNVNED 165
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V4BIY8 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_165460 PE=4 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.341e-14
Identity = 45/115 (39.13%), Postives = 69/115 (60.00%), Query Frame = 2 
Query: 1001 PAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNE 1345
            P  KY GE H+PG+N+ GPGTR DLRLD + KP P   PVN +    + HDI Y +K    R+ +D ++I  LEN+   N + ++++ R LI+ +  LK+  ++  GL   ++ E
Sbjct:   27 PWAKYPGEKHLPGHNYTGPGTRLDLRLDENDKPKPGEEPVNRVDAAALKHDILYRNKDIQFRHEADKQMIIELENI--PNPTFKERMERALIVKL--LKAKMKLGMGLADEIHKE 137
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V3ZC14 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_165366 PE=4 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.609e-13
Identity = 46/116 (39.66%), Postives = 69/116 (59.48%), Query Frame = 2 
Query:  998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNE 1345
             P  KY GE H+PG+N+ GPGTR DLRLD + KP P   PVN +    + HDI Y +K    R+ +D ++I  LEN+   N + ++++ R LII +  LK+  ++  GL   ++ E
Sbjct:   26 FPWAKYPGEKHLPGHNYTGPGTRLDLRLDENDKPKPGEEPVNRVDAAALKHDILYRNKDINFRHEADRQMIIVLENI--PNPTFKERMQRALIIKL--LKAKMKLGMGLADEIHKE 137
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Nematostella
Match: EDO44700 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUZ8])

HSP 1 Score: 48.9062 bits (115), Expect = 6.731e-6
Identity = 32/91 (35.16%), Postives = 50/91 (54.95%), Query Frame = 2 
Query: 1019 GEMHVP-----GYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYH-SDLRLIKALENMDTKNLSKQDKIVRRL 1273
            GE+H+       YN+CGPGTR   RL++    +  S P+N +  LC  HDI Y+    L   H +D  L++A+ N+  KN+  +   +RR+
Sbjct:   19 GEIHLRTPTGLSYNYCGPGTRLRQRLNSSNPGI--SNPINILDALCQKHDIAYSKSKDLDDKHVADDNLLEAISNIP-KNMQHKKTPLRRV 106
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000012349.1 (SMESG000012349.1)

HSP 1 Score: 172.555 bits (436), Expect = 7.092e-50
Identity = 77/107 (71.96%), Postives = 97/107 (90.65%), Query Frame = 2 
Query: 1025 MHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNE 1345
            MHVPGYNFCGPGTRTDLRLD +KKPVP SLP+N+I ELCVAHDI+Y +++RLVRY SDL+LIKAL++M T+N+SKQDK+VR+LII VF+LK+NFE++AGLIK +  +
Sbjct:    1 MHVPGYNFCGPGTRTDLRLDKNKKPVPTSLPINSIDELCVAHDIEYGNQNRLVRYKSDLKLIKALQDMATENMSKQDKLVRKLIIYVFKLKTNFEVLAGLIKDVEKD 107
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000000949.1 (SMESG000000949.1)

HSP 1 Score: 113.235 bits (282), Expect = 1.135e-28
Identity = 64/147 (43.54%), Postives = 92/147 (62.59%), Query Frame = 2 
Query:  998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQDLRLFM 1438
            MP +K  GEMH+PGYNF GPGTRTDLRLD  KKPV  S P+N I E C+ HDI YN K + VRY SD +LI  ++ +  ++ + ++++V  LII VF LK+ FE   G++     EN+   L   + E +K +  N++ +  +  F+
Sbjct:    1 MPKLK--GEMHIPGYNFWGPGTRTDLRLDEHKKPVSWSKPINKIDEQCLTHDIAYNSKDKRVRYDSDRQLI--MDILYLRHKTPEEEVVGNLIIYVFELKNIFEKKLGIV-----ENQ--HLTKEEQEQMKNYVRNLEYSTTIAKFL 136
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000003420.1 (SMESG000003420.1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.467e-11
Identity = 30/74 (40.54%), Postives = 44/74 (59.46%), Query Frame = 2 
Query:  998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKAL 1219
             P  KY GE H+P +N+ GP TR D+RLD++  P P   P+N + E  + HDI Y +++   R   D+ LI+ L
Sbjct:   30 FPWSKYTGEHHLPKFNYLGPRTRLDIRLDSNDIPKPGEEPINRVDEDALRHDIAYRNENLRNRQKPDIDLIEDL 103
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000030763.1 (SMESG000030763.1)

HSP 1 Score: 56.225 bits (134), Expect = 5.422e-9
Identity = 27/69 (39.13%), Postives = 47/69 (68.12%), Query Frame = -2 
Query:  162 SEITQHVDKLDYPGS---RRYNSFQFNISVIKYRQLEASLSKTFQDSSFTRFVVGQEEEGDAGFRHMQI 359
            SE+ +HV+ ++ P     RR+NSF+F++S+ KY++LE+ L   + D + + FV+GQ+   D+G  HMQ+
Sbjct:   59 SELAEHVE-IEIPDKQRQRRHNSFKFSVSMRKYKELESHLKALWADKTTSHFVIGQD---DSGHHHMQM 123
[Close]
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000052617.1 (SMESG000052617.1)

HSP 1 Score: 51.9878 bits (123), Expect = 3.778e-7
Identity = 25/73 (34.25%), Postives = 41/73 (56.16%), Query Frame = 2 
Query: 1010 KYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENM 1228
            KY  E H+ G++F G GTR D+RLD +    P   P+N + +  + HDI Y ++    R  + + LI+ L ++
Sbjct:   39 KYPDERHLIGHSFTGSGTRLDIRLDENDNSKPGEEPINRVDQAALKHDIAYRNEDIRSRQKAYIDLIQNLNDV 111
The following BLAST results are available for this feature:
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V3ZPR97.746e-1543.43Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4AHX31.268e-1433.52Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4AAM02.106e-1434.48Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4BIY84.341e-1439.13Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V3ZC141.609e-1339.66Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO447006.731e-635.16Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000012349.17.092e-5071.96SMESG000012349.1[more]
SMESG000000949.11.135e-2843.54SMESG000000949.1[more]
SMESG000003420.11.467e-1140.54SMESG000003420.1[more]
SMESG000030763.15.422e-939.13SMESG000030763.1[more]
SMESG000052617.13.778e-734.25SMESG000052617.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004312 ID=SMED30004312|Name=Parvo_coat_N domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=4260bp
TTTGTATATTTAAAAAATTCAGAATTTTAAACCATATCACGTTGTAATTA
ACTTAAATTTAATTACAATTAATTTTGGTGTATTCTGGCACATTTGTTGA
CACTAATTCTACGCTTGATTAATCAACGATGAAAAAATATTCAATTCGCA
AATAGCAATGAGATCTGCATATGCCGGAATCCAGCATCACCCTCCTCTTC
CTGTCCGACCACGAAACGGGTAAAGGAACTGTCCTGGAAAGTTTTCGATA
GACTAGCTTCCAATTGTCGATATTTGATGACAGATATGTTAAACTGGAAG
CTGTTATATCTTCTCGATCCTGGGTAATCGAGTTTGTCAACATGTTGAGT
GATTTCAGATTGAAGATCGTTCTCTGCAAGCACCGAACTATTTCCATTAG
GAGAATTGGGTCCTGATACGTATTCAGAATGTGCTTGTAACTCCTGCTCA
GCCAGTCGAGGGACTTTACATAGCTGTTCACGCGATGGACTAATTCGCAG
ATTGGGCAGATTTGTCGAACGCACATCGGCACAATCCTTGTCTGTGCTTC
TTTTGTTGCGAATATTACTGTTGGTGCTCGATTCCCCATTCTGTACAAAT
TGTCTATTTCCTCGATCAACACTTGCACCTCTGACCCTCCGATGTGTTGT
CTGAATTGAGGTGTCACGTTCATCAAAATTCATGCTGAAGATTGGGTTGT
TCAGGAAATATGTCCATTGGTCGGAATAATCGTGTTCAAAAAGTATTGCT
AATAAAACATTAATAATTGATTAACAAAAAAATATTACACAAAGCTTACT
ATCGATTTTATCTAGGATGATAAATAACTGGTTCGCGTGCTGACCAATCA
GCAGTGGATGATACTTGTCCAATTGGGATTTATATGCGTTGAATAGTCCT
GCCAAATAGTATATTGGTTCACAAGTTTCCACAGAGACGTTAACGGAGAT
ACTAGATAATTTTTCAATTGCTCACAATTTCGATATTGCTCTTCAAAATG
CCTGCGATTAAATACCGAGGCGAGATGCATGTGCCAGGTTATAACTTCTG
TGGTCCTGGCACACGAACTGATCTCAGATTGGATGCCGATAAGAAACCTG
TTCCAGCAAGTCTACCAGTTAATGCTATCGTTGAGTTGTGCGTTGCTCAC
GATATAGATTATAACGACAAGAGCAGACTTGTTCGTTATCATTCCGATTT
GCGACTTATCAAAGCACTTGAAAACATGGATACTAAAAACCTGTCTAAAC
AGGATAAAATTGTAAGAAGATTAATCATTTAGGTTTTTAGATTAAAATCA
AACTTTGAAATTATGGCCGGTTTAATTAAAGGTCTGAATAATGAAAATAA
AGATATTAAATTGAATATTACTGATGTCGAATTAATTAAAAGATTTTCAG
AAAATATAAAAGTAGCACAGGATTTGAGGCTGTTTATGAAATCACATTTT
GAGCAACAAGGAAATGTAGGTTTTGTTGAACGGGACCGTTTAAATGCAAT
TAATGCGACAGCACAATTATTCAATGTCTTTGCAATAACTACCTTTGGGA
AAGTGCAAGGAGTTTTGAGGCAATCTAACATGAAATCATCATTTAAATCA
ACTCCATACCAACAACTGTTGTTATCTAATTCACTTAAATCTTTAAGTAG
TGATTTATTTAAGCCCTTAAAGATGAATCAAACATTTAAAAATAACATAT
TAAATTATACTGTCCGCAATATACTACGCCCAGGTATGAAAGGATTTATA
AGAAAAATTGGAGGAGTTCGTCAAAATGCTCATGACATAGCTGCTATTGC
TGGATTTACAATTGGATTTATATCAGTGCATATATTAGAAGTTTTTATGT
TCAACCTTTGGAATTACTAGTGAAAGACCACATAAAACCTTATACTAATA
AACATTTATTACAAGCTTTTAATGAACTATCTAACCATGACGGGTTTGTT
GACGTTACGGAATTATTTGGAAATGATTTAAAGTTAAATGATTACAGGTT
ATTTGAGAATAAAGATTTTGAAGATATTTATCAGCCCAATAGGCAAAAAG
AGTTCAAAGATTTGTTAACTTTATTTGACACCGAAATGGCTGAAATTTCT
ATTTATAATGGTATCAAGTTACTAGAAAGCAAACTGTTACATGAATACAA
AGAAGGAGATGATATATATGACTTAATAAGAAAAGATGATGATATGAGAA
GGTTATTAGATGATAATGTTGGTTTATATGAGCAGCTAGTAGAAGGCAGT
AAAAACTTCAAGAATCAAAAATTCTTGGGTTTAATAAATCAATATGAAAG
ACATTGGGGGCCTGATGTTGGAAAACCTGGTGACTTACATAAATATGTTA
ATTTTTATACTAATATGTGGTATCATTACCATTACTCGCAGGGTACTTTA
AATGACCCTAAACATCTGCCGTTTGGTTACATGGGTAAGCGTCGATTAGA
AGAAATTATATCTAGATTTCCATACGATGCAGTAAGAAAGAAAAGAAACG
TAAACTTCAGAGCACACTCGGTTAGAATTGAATAAGAAATTTGATGATTA
TTTAAAGTTAAATTCCAAAGCCTACCACAAGGCACCATTATTGAAAATAA
AGGGGATACCAGACGAATATCCCAGACGTCTTAGTGCTATACAGTTGAAT
GTGTTACGTTATTATTATCACAATAACCATCACAGATCTGCAACGCATGA
GGAATGGAAACAGATAGTCGACGAAAAAATAAAAAAATATGACACACACA
CTGAATCCCTCGAATTAGATATTGATGATAGAAATAAATTACATTGGTTT
TTAAGTGAACATCTTTTGCAAACACACGAAGACGGAGTGACAAAATATCG
AGGCACACTACCTGCTATAAATGCGTTCAACAATATGTTTTTGGGCATTA
ATATGACTACTCATGCACCTAATACAACTACGGCGCCAGTGGTACCACCC
TCTGCTATCCCCGGCAGTGGGGGAGGTAATGGAATACTTAGATCTCAACA
AGGAGAATTCAGACCATCTTTGGACAACAACTCCCTTACCTTCACCAGCA
CCAATTGCGGAGCTTCTTGCATTGAAACCACCGCAACAGGAGAATGGTAT
AATTTCCCAGTTGAATATTATTACCCTCATACGATGTATGGTAATCCGGA
TTTCTGGGTTTCGATGGAGAGATATTTATGGTTTAAACCTGTCGCTATGG
AATTTGAGATGTTTAATCTACAAGTTGTGGGTGAAACTTCGCCAACGAAC
TTCTCAGGACTTCAAAACCAAGTAGAATTACTTGTATTTCGTGACGAAGC
CAATATATATGGGCCATGCGCTGCGCCACCACAGATGGCAAAAGCATTTA
AAGATATTTTTCTTAACGAAGCATCTCTTCATAATGGATATAATATCAAT
AACCAATTGTCTAAGCTCACTCCAATAACGTTACCTAAAGCTGGATGGAT
ATACTATCCCGAGACGTGTCCTGAAGTTGAAATAATTAATTGCAATACAT
CAGAAACTGTAAATTATTCTTATCAAGCATCGAAGGTACAACATCCAAGA
TATATTCGAGAGTTTTGGGATCAACATTTAGATTTAACAATTGCAAACGA
CAATGATCATATTAAATGGCACAGGTTTGATTACTTTAATTGTAAAAAAA
TTAAACCTTTTTCTTATGCTAATGCTCTTGGGAGAAGAACTATTACAGCT
GATAAAAGAGTTATGCCATTACCATCAAAAATATATTGCCCTAAATGGAA
GAGAAAGTGGGATCCAAAACTGAAGCGACAACATTACGATTATGTACTAG
GAGATATGACAATACATAGGAAAATATTGCCTACAGACAAAACAGATATT
ATCGATGATTGGTATACTGCTCGACGTGAAGCTCTTGAGGAGTATCGGAG
TCATTGTGCACAAGAAGATGTATCCGAGCATAACCCAATGAAAATGATGT
TCTTTAAACTAAACACAATGATTGGTCCTGGTGGAAACAAGATCAATTTG
ACATGTACCTTTAATTGGAAAATAAAATATACATTTGAATTATTTGGGAA
ATTACCATCTACAAACATTAGGCAAATTTATCCTATAACAGATCCTATTT
TAAAGGGACAAACTAATTGGCAACCGACAACTAAATGGTTAACACCTTCA
ATTCCTTACTGTACACCATATTTTTTCTGTATTCGAGATGAAGGAACTGA
AGAGGGAGAGGATGAATTGTTTCCAGTGATTAAGCATAATGCAGCTGGAT
TTAAGTGTCC
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protein sequence of SMED30004312-orf-1

>SMED30004312-orf-1 ID=SMED30004312-orf-1|Name=SMED30004312-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MAEISIYNGIKLLESKLLHEYKEGDDIYDLIRKDDDMRRLLDDNVGLYEQ
LVEGSKNFKNQKFLGLINQYERHWGPDVGKPGDLHKYVNFYTNMWYHYHY
SQGTLNDPKHLPFGYMGKRRLEEIISRFPYDAVRKKRNVNFRAHSVRIE*
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protein sequence of SMED30004312-orf-2

>SMED30004312-orf-2 ID=SMED30004312-orf-2|Name=SMED30004312-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=181bp
MNFDERDTSIQTTHRRVRGASVDRGNRQFVQNGESSTNSNIRNKRSTDKD
CADVRSTNLPNLRISPSREQLCKVPRLAEQELQAHSEYVSGPNSPNGNSS
VLAENDLQSEITQHVDKLDYPGSRRYNSFQFNISVIKYRQLEASLSKTFQ
DSSFTRFVVGQEEEGDAGFRHMQISLLFAN*
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protein sequence of SMED30004312-orf-3

>SMED30004312-orf-3 ID=SMED30004312-orf-3|Name=SMED30004312-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=186bp
MAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQDLRLFMKSHFEQQG
NVGFVERDRLNAINATAQLFNVFAITTFGKVQGVLRQSNMKSSFKSTPYQ
QLLLSNSLKSLSSDLFKPLKMNQTFKNNILNYTVRNILRPGMKGFIRKIG
GVRQNAHDIAAIAGFTIGFISVHILEVFMFNLWNY*
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protein sequence of SMED30004312-orf-4

>SMED30004312-orf-4 ID=SMED30004312-orf-4|Name=SMED30004312-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=458bp
MFLGINMTTHAPNTTTAPVVPPSAIPGSGGGNGILRSQQGEFRPSLDNNS
LTFTSTNCGASCIETTATGEWYNFPVEYYYPHTMYGNPDFWVSMERYLWF
KPVAMEFEMFNLQVVGETSPTNFSGLQNQVELLVFRDEANIYGPCAAPPQ
MAKAFKDIFLNEASLHNGYNINNQLSKLTPITLPKAGWIYYPETCPEVEI
INCNTSETVNYSYQASKVQHPRYIREFWDQHLDLTIANDNDHIKWHRFDY
FNCKKIKPFSYANALGRRTITADKRVMPLPSKIYCPKWKRKWDPKLKRQH
YDYVLGDMTIHRKILPTDKTDIIDDWYTARREALEEYRSHCAQEDVSEHN
PMKMMFFKLNTMIGPGGNKINLTCTFNWKIKYTFELFGKLPSTNIRQIYP
ITDPILKGQTNWQPTTKWLTPSIPYCTPYFFCIRDEGTEEGEDELFPVIK
HNAAGFKC
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004623phospholipase A2 activity
GO:0005198structural molecule activity
Vocabulary: biological process
TermDefinition
GO:0006644phospholipid metabolic process
GO:0050482arachidonic acid secretion
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000035Category 3 cell