Parvo_coat_N domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004312 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V3ZPR9 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_155882 PE=4 SV=1) HSP 1 Score: 85.5001 bits (210), Expect = 7.746e-15 Identity = 43/99 (43.43%), Postives = 61/99 (61.62%), Query Frame = 2 Query: 1001 PAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLK 1297 P KY GE H+PG+N+ GPGTR DLRLD + KP P PVN + + HDI Y +K R+ +D ++I LEN+ N + ++K+ R LII + + K Sbjct: 106 PWAKYSGEKHLPGHNYTGPGTRLDLRLDQNDKPKPGEEPVNRVDAAALKHDILYRNKDLKFRHEADKQMIIELENI--PNPTFKEKMQRALIIKLLKTK 202
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V4AHX3 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_162020 PE=4 SV=1) HSP 1 Score: 87.8113 bits (216), Expect = 1.268e-14 Identity = 61/182 (33.52%), Postives = 97/182 (53.30%), Query Frame = 2 Query: 998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQDLRLFMKSHFEQQGNVGFVERDRLNAINATAQLFNVFAITT 1543 P KY GE H+PG+N+ GPGTR DLRLD + P P PVN + + HDI Y +K R+ +D ++I LEN+ N + ++K+ R LII + + K + + + L+ + D +L +V +IK F +N R MK++ ++Q N+ V R + + +L + AI T Sbjct: 28 FPWAKYPGEKHLPGHNYTGPGTRLDLRLDENDIPKPGEEPVNRVDAAALKHDILYRNKDVTFRHQADKQMIDELENI--PNPTFKEKLQRALIIKLMKAKLKLDTLKQQLTALDTKITD-ELE-KEVGVIKNFLQN-----KFRDDMKNYIKEQVNL-LVSRIHDDFLTQEKKLSKLEAIVT 199
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V4AAM0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_172863 PE=4 SV=1) HSP 1 Score: 83.9593 bits (206), Expect = 2.106e-14 Identity = 50/145 (34.48%), Postives = 79/145 (54.48%), Query Frame = 2 Query: 1001 PAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNF----EIMAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQD 1423 P KY GE H+PG+N+ GPGTR DLRLD + P P PVN + + HDI Y +K R+ +D ++I LEN+ N + ++K+ R LII + + K + LI L+++ +K++ ++R +N V +D Sbjct: 29 PWAKYPGEKHLPGHNYTGPGTRLDLRLDENDNPKPGEEPVNRVDAAALKHDILYRNKDITFRHQADKQMIDELENI--PNPTFKEKLQRALIIKLLKAKVKLVNKTSQLQNLISKLDDKITKVKID------VQRLIDNPNVNED 165
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V4BIY8 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_165460 PE=4 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 4.341e-14 Identity = 45/115 (39.13%), Postives = 69/115 (60.00%), Query Frame = 2 Query: 1001 PAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNE 1345 P KY GE H+PG+N+ GPGTR DLRLD + KP P PVN + + HDI Y +K R+ +D ++I LEN+ N + ++++ R LI+ + LK+ ++ GL ++ E Sbjct: 27 PWAKYPGEKHLPGHNYTGPGTRLDLRLDENDKPKPGEEPVNRVDAAALKHDILYRNKDIQFRHEADKQMIIELENI--PNPTFKERMERALIVKL--LKAKMKLGMGLADEIHKE 137
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Match: V3ZC14 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_165366 PE=4 SV=1) HSP 1 Score: 84.3445 bits (207), Expect = 1.609e-13 Identity = 46/116 (39.66%), Postives = 69/116 (59.48%), Query Frame = 2 Query: 998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNE 1345 P KY GE H+PG+N+ GPGTR DLRLD + KP P PVN + + HDI Y +K R+ +D ++I LEN+ N + ++++ R LII + LK+ ++ GL ++ E Sbjct: 26 FPWAKYPGEKHLPGHNYTGPGTRLDLRLDENDKPKPGEEPVNRVDAAALKHDILYRNKDINFRHEADRQMIIVLENI--PNPTFKERMQRALIIKL--LKAKMKLGMGLADEIHKE 137
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Nematostella
Match: EDO44700 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUZ8]) HSP 1 Score: 48.9062 bits (115), Expect = 6.731e-6 Identity = 32/91 (35.16%), Postives = 50/91 (54.95%), Query Frame = 2 Query: 1019 GEMHVP-----GYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYH-SDLRLIKALENMDTKNLSKQDKIVRRL 1273 GE+H+ YN+CGPGTR RL++ + S P+N + LC HDI Y+ L H +D L++A+ N+ KN+ + +RR+ Sbjct: 19 GEIHLRTPTGLSYNYCGPGTRLRQRLNSSNPGI--SNPINILDALCQKHDIAYSKSKDLDDKHVADDNLLEAISNIP-KNMQHKKTPLRRV 106
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000012349.1 (SMESG000012349.1) HSP 1 Score: 172.555 bits (436), Expect = 7.092e-50 Identity = 77/107 (71.96%), Postives = 97/107 (90.65%), Query Frame = 2 Query: 1025 MHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNE 1345 MHVPGYNFCGPGTRTDLRLD +KKPVP SLP+N+I ELCVAHDI+Y +++RLVRY SDL+LIKAL++M T+N+SKQDK+VR+LII VF+LK+NFE++AGLIK + + Sbjct: 1 MHVPGYNFCGPGTRTDLRLDKNKKPVPTSLPINSIDELCVAHDIEYGNQNRLVRYKSDLKLIKALQDMATENMSKQDKLVRKLIIYVFKLKTNFEVLAGLIKDVEKD 107
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000000949.1 (SMESG000000949.1) HSP 1 Score: 113.235 bits (282), Expect = 1.135e-28 Identity = 64/147 (43.54%), Postives = 92/147 (62.59%), Query Frame = 2 Query: 998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENMDTKNLSKQDKIVRRLII*VFRLKSNFEIMAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQDLRLFM 1438 MP +K GEMH+PGYNF GPGTRTDLRLD KKPV S P+N I E C+ HDI YN K + VRY SD +LI ++ + ++ + ++++V LII VF LK+ FE G++ EN+ L + E +K + N++ + + F+ Sbjct: 1 MPKLK--GEMHIPGYNFWGPGTRTDLRLDEHKKPVSWSKPINKIDEQCLTHDIAYNSKDKRVRYDSDRQLI--MDILYLRHKTPEEEVVGNLIIYVFELKNIFEKKLGIV-----ENQ--HLTKEEQEQMKNYVRNLEYSTTIAKFL 136
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000003420.1 (SMESG000003420.1) HSP 1 Score: 62.7734 bits (151), Expect = 1.467e-11 Identity = 30/74 (40.54%), Postives = 44/74 (59.46%), Query Frame = 2 Query: 998 MPAIKYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKAL 1219 P KY GE H+P +N+ GP TR D+RLD++ P P P+N + E + HDI Y +++ R D+ LI+ L Sbjct: 30 FPWSKYTGEHHLPKFNYLGPRTRLDIRLDSNDIPKPGEEPINRVDEDALRHDIAYRNENLRNRQKPDIDLIEDL 103
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000030763.1 (SMESG000030763.1) HSP 1 Score: 56.225 bits (134), Expect = 5.422e-9 Identity = 27/69 (39.13%), Postives = 47/69 (68.12%), Query Frame = -2 Query: 162 SEITQHVDKLDYPGS---RRYNSFQFNISVIKYRQLEASLSKTFQDSSFTRFVVGQEEEGDAGFRHMQI 359 SE+ +HV+ ++ P RR+NSF+F++S+ KY++LE+ L + D + + FV+GQ+ D+G HMQ+ Sbjct: 59 SELAEHVE-IEIPDKQRQRRHNSFKFSVSMRKYKELESHLKALWADKTTSHFVIGQD---DSGHHHMQM 123
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Match: SMESG000052617.1 (SMESG000052617.1) HSP 1 Score: 51.9878 bits (123), Expect = 3.778e-7 Identity = 25/73 (34.25%), Postives = 41/73 (56.16%), Query Frame = 2 Query: 1010 KYRGEMHVPGYNFCGPGTRTDLRLDADKKPVPASLPVNAIVELCVAHDIDYNDKSRLVRYHSDLRLIKALENM 1228 KY E H+ G++F G GTR D+RLD + P P+N + + + HDI Y ++ R + + LI+ L ++ Sbjct: 39 KYPDERHLIGHSFTGSGTRLDIRLDENDNSKPGEEPINRVDQAALKHDIAYRNEDIRSRQKAYIDLIQNLNDV 111 The following BLAST results are available for this feature:
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Parvo_coat_N domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Parvo_coat_N domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004312 ID=SMED30004312|Name=Parvo_coat_N domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=4260bpback to top protein sequence of SMED30004312-orf-1 >SMED30004312-orf-1 ID=SMED30004312-orf-1|Name=SMED30004312-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp MAEISIYNGIKLLESKLLHEYKEGDDIYDLIRKDDDMRRLLDDNVGLYEQback to top protein sequence of SMED30004312-orf-2 >SMED30004312-orf-2 ID=SMED30004312-orf-2|Name=SMED30004312-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=181bp MNFDERDTSIQTTHRRVRGASVDRGNRQFVQNGESSTNSNIRNKRSTDKDback to top protein sequence of SMED30004312-orf-3 >SMED30004312-orf-3 ID=SMED30004312-orf-3|Name=SMED30004312-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=186bp MAGLIKGLNNENKDIKLNITDVELIKRFSENIKVAQDLRLFMKSHFEQQGback to top protein sequence of SMED30004312-orf-4 >SMED30004312-orf-4 ID=SMED30004312-orf-4|Name=SMED30004312-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=458bp MFLGINMTTHAPNTTTAPVVPPSAIPGSGGGNGILRSQQGEFRPSLDNNSback to top Annotated Terms
The following terms have been associated with this transcript:
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