SMED30004300
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004300 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30004300 aligns in the following genomic locations:
Homology
BLAST of SMED30004300 vs. Planmine SMEST
Match: SMESG000027003.1 (SMESG000027003.1) HSP 1 Score: 1065.06 bits (2753), Expect = 0.000e+0 Identity = 639/1043 (61.27%), Postives = 751/1043 (72.00%), Query Frame = 2 Query: 47 MAWQCKHSLFMLCTRPSTRKECALDVANKLIDMVVHSYIMKSIARPLTTELAMMDFSHKDLFMSDFMKELCNFKSIEDLLVNMSMKYSSLTPIKQNVEDIKVKDSDRSDETELKLNKFLSGITESELNIPQIEGEQHVSIKEVEFKAGQIIDEPLSRIEEKNEPIIIDDSSKQNKEEAILHVSVDKLKEIKSSCIDSELNVGNLKYPSVSEKIVNQNKDANLDNYLQSLLNSEQKEDNLHDIQEDEVVQKNKTSNVTISQPDEPCVPFTFESDIYHVERTEEISKDFEEQYNSYEVXXXXXXXXXXXXXXXXXXXXCLLNSQRDEETFPDIHDDNQVRMSESSGCIVSESKGEHMSSSEDYMRSVCYINETNLTSDIILERDFFDNETKFKADEITNKNLERIPANEDYPLVIDNLTIKDRNLTMEEKDVISNSSAKKQSFTKESEVKYEVSETKFLALDKKDSCKSFPLDYSSNADIISEKFIDYPLVVGHQSIRDETLIREDKDLIADSSGKKESFTKESQVKYEVSEIASLTLDKKESCKSFPVDYSSNVCIISEKVVDQTFDNKSKSVEIMERNEEALIIDSDLKFDLCQTLVTTSVEKEVSHGSMDLERYLVKDKILDEPEHLNSQQDLKPTGFDQQRDEDNRVINTEVADETKLKFEQEALKNDQISLSIIRENKDFMSSQKEIEFETCQTVDINSIQKQESYSSF-------KAQIQIDAENMSTQQDGQNLNHPLITDLENNENTLIQNTDASMEIYQTPEMSSGKSKESGLYFVLEDP-FMHNESKEKTEDRNIPD-DQLPKKTSEKSNESGLYFVLEDPFLLSKNTMKTDENILPDQVSLSIVQENKDFQNSKNEIEFEACQSTDLLSNQKQESYSSFKLDHEIREDNVPEQLDDQSSLVNPLWISNIDETEKSPIQNTDVNFEICQIPEIQVTLTEKSNLSYVVEDSVIPNQSTLKTEDDNIQNDPMSLMVVQGRQDLLTSHNEVEFEACQTNDLHLNKRNERYSSLKADNNIEEDPFTTRLEERDLETKPL 3148 MAWQCKHSLFMLCTRPSTRKECALDVANKLIDMVVHSYIMKSIARPLTTELAMMDFSHKDLFMSDFMKELCNFKSIEDLLVNMSMKYSSLTPIKQNVEDIKVKDSDRSDETELKLNKFLSGITESELNIPQIEGEQHVSIKEVEFKAGQIIDEPLSRIEEKNEPIIIDDSSKQNKEEAILHVSVDKLKEIKSSCIDSELNVGNLKYPSVSEKIVNQNKDANLDNYLQSLLNSEQKEDNLHDIQEDEVVQKNKTSNVTISQPDEPCVPFTFESDIYHVERTEEISKDFEEQYNSYEVNDETKLLNKNKDKTLENDLKCLLNSQRDEETFPDIHDDNQVRMSESSGCIVSESKGEHMSSSEDYMRSVCYINETNLTSDIILERDFFDNETKFKADEITNKNLERIPANEDYPLVIDNLTIKDRNLTMEEKDVISNSSAKKQSFTKESEVKYEVSETKFLALDKKDSCKSFPLDYSSNADIISEKFIDYPLVVGHQSIRDETLIREDKDLIADSSGKKESFTKES+++ +V + ++ KE DY N+ + + ++K K + E +E+ +S +K+++ + T LD+ E S F + +I+ +V D+T N S+ I+ N++ + +++F+ CQT+ S++K+ S+ S K +I + E++++QQD + P D + +E+ + NT+ + E E + K+ + L + E+ FM ++ + + E D + + K+ S S ++ + E+ +T + +N+ ++ EN QN+ + E Q+ ++ S + +ES F L+ + E+ +D+ N Q+P+ + +S L +V+ED + +++T+KT D+NI D +SL +VQ +D S NE+EFEACQ+ DL N++ E YSS K D+ I ED +L+++ PL Sbjct: 1 MAWQCKHSLFMLCTRPSTRKECALDVANKLIDMVVHSYIMKSIARPLTTELAMMDFSHKDLFMSDFMKELCNFKSIEDLLVNMSMKYSSLTPIKQNVEDIKVKDSDRSDETELKLNKFLSGITESELNIPQIEGEQHVSIKEVEFKAGQIIDEPLSRIEEKNEPIIIDDSSKQNKEEAILHVSVDKLKEIKSSCIDSELNVGNLKYPSVSEKIVNQNKDANLDNYLQSLLNSEQKEDNLHDIQEDEVVQKNKTSNVTISQPDEPCVPFTFESDIYHVERTEEISKDFEEQYNSYEVNDETKLLNKNKDKTLENDLKCLLNSQRDEETFPDIHDDNQVRMSESSGCIVSESKGEHMSSSEDYMRSVCYINETNLTSDIILERDFFDNETKFKADEITNKNLERIPANEDYPLVIDNLTIKDRNLTMEEKDVISNSSAKKQSFTKESEVKYEVSETKFLALDKKDSCKSFPLDYSSNADIISEKFIDYPLVVGHQSIRDETLIREDKDLIADSSGKKESFTKESEIRRKVKSLNPESMSDKE-------DYPLNIDNLPTSGENLIVEDKYK-LSDSEGKKESFTKESQVKYEVSEIASLT----------------------LDKKESCKS--------FPVDYSSNVCIISEKVVDQT--------FDNKSKSVEIMERNEEALIIDSDLKFDLCQTLVTTSVEKEVSHGSMDLERYLVKDKILDEPEHLNSQQDLK----PTGFDQQRDEDNRVINTEVADETKLKFEQEALKNDQISLSIIRENKDFMSSQKEIEFETCQTVDINSIQKQESYSSFKAQIQIDAEN-----MSTQQDGQNLNHPLITDLENNENTLIQNT--DASMEIYQTPEMSSGKSKESGLYFVLEDPFMHNESKEKTEDR-------------------------NIPDDQLPKKTSEKSNESGLYFVLEDPFLLSKNTMKT-DENILPDQVSLSIVQENKDFQNSKNEIEFEACQSTDLLSNQKQESYSSFKLDHEIREDNVPEQLDDQSSLVNPL 960
BLAST of SMED30004300 vs. Planmine SMEST
Match: SMESG000027003.1 (SMESG000027003.1) HSP 1 Score: 1038.48 bits (2684), Expect = 0.000e+0 Identity = 629/1033 (60.89%), Postives = 741/1033 (71.73%), Query Frame = 2 Query: 77 MLCTRPSTRKECALDVANKLIDMVVHSYIMKSIARPLTTELAMMDFSHKDLFMSDFMKELCNFKSIEDLLVNMSMKYSSLTPIKQNVEDIKVKDSDRSDETELKLNKFLSGITESELNIPQIEGEQHVSIKEVEFKAGQIIDEPLSRIEEKNEPIIIDDSSKQNKEEAILHVSVDKLKEIKSSCIDSELNVGNLKYPSVSEKIVNQNKDANLDNYLQSLLNSEQKEDNLHDIQEDEVVQKNKTSNVTISQPDEPCVPFTFESDIYHVERTEEISKDFEEQYNSYEVXXXXXXXXXXXXXXXXXXXXCLLNSQRDEETFPDIHDDNQVRMSESSGCIVSESKGEHMSSSEDYMRSVCYINETNLTSDIILERDFFDNETKFKADEITNKNLERIPANEDYPLVIDNLTIKDRNLTMEEKDVISNSSAKKQSFTKESEVKYEVSETKFLALDKKDSCKSFPLDYSSNADIISEKFIDYPLVVGHQSIRDETLIREDKDLIADSSGKKESFTKESQVKYEVSEIASLTLDKKESCKSFPVDYSSNVCIISEKVVDQTFDNKSKSVEIMERNEEALIIDSDLKFDLCQTLVTTSVEKEVSHGSMDLERYLVKDKILDEPEHLNSQQDLKPTGFDQQRDEDNRVINTEVADETKLKFEQEALKNDQISLSIIRENKDFMSSQKEIEFETCQTVDINSIQKQESYSSF-------KAQIQIDAENMSTQQDGQNLNHPLITDLENNENTLIQNTDASMEIYQTPEMSSGKSKESGLYFVLEDP-FMHNESKEKTEDRNIPD-DQLPKKTSEKSNESGLYFVLEDPFLLSKNTMKTDENILPDQVSLSIVQENKDFQNSKNEIEFEACQSTDLLSNQKQESYSSFKLDHEIREDNVPEQLDDQSSLVNPLWISNIDETEKSPIQNTDVNFEICQIPEIQVTLTEKSNLSYVVEDSVIPNQSTLKTEDDNIQNDPMSLMVVQGRQDLLTSHNEVEFEACQTNDLHLNKRNERYSSLKADNNIEEDPFTTRLEERDLETKPL 3148 MLCTRPSTRKECALDVANKLIDMVVHSYIMKSIARPLTTELAMMDFSHKDLFMSDFMKELCNFKSIEDLLVNMSMKYSSLTPIKQNVEDIKVKDSDRSDETELKLNKFLSGITESELNIPQIEGEQHVSIKEVEFKAGQIIDEPLSRIEEKNEPIIIDDSSKQNKEEAILHVSVDKLKEIKSSCIDSELNVGNLKYPSVSEKIVNQNKDANLDNYLQSLLNSEQKEDNLHDIQEDEVVQKNKTSNVTISQPDEPCVPFTFESDIYHVERTEEISKDFEEQYNSYEVNDETKLLNKNKDKTLENDLKCLLNSQRDEETFPDIHDDNQVRMSESSGCIVSESKGEHMSSSEDYMRSVCYINETNLTSDIILERDFFDNETKFKADEITNKNLERIPANEDYPLVIDNLTIKDRNLTMEEKDVISNSSAKKQSFTKESEVKYEVSETKFLALDKKDSCKSFPLDYSSNADIISEKFIDYPLVVGHQSIRDETLIREDKDLIADSSGKKESFTKES+++ +V + ++ KE DY N+ + + ++K K + E +E+ +S +K+++ + T LD+ E S F + +I+ +V D+T N S+ I+ N++ + +++F+ CQT+ S++K+ S+ S K +I + E++++QQD + P D + +E+ + NT+ + E E + K+ + L + E+ FM ++ + + E D + + K+ S S ++ + E+ +T + +N+ ++ EN QN+ + E Q+ ++ S + +ES F L+ + E+ +D+ N Q+P+ + +S L +V+ED + +++T+KT D+NI D +SL +VQ +D S NE+EFEACQ+ DL N++ E YSS K D+ I ED +L+++ PL Sbjct: 1 MLCTRPSTRKECALDVANKLIDMVVHSYIMKSIARPLTTELAMMDFSHKDLFMSDFMKELCNFKSIEDLLVNMSMKYSSLTPIKQNVEDIKVKDSDRSDETELKLNKFLSGITESELNIPQIEGEQHVSIKEVEFKAGQIIDEPLSRIEEKNEPIIIDDSSKQNKEEAILHVSVDKLKEIKSSCIDSELNVGNLKYPSVSEKIVNQNKDANLDNYLQSLLNSEQKEDNLHDIQEDEVVQKNKTSNVTISQPDEPCVPFTFESDIYHVERTEEISKDFEEQYNSYEVNDETKLLNKNKDKTLENDLKCLLNSQRDEETFPDIHDDNQVRMSESSGCIVSESKGEHMSSSEDYMRSVCYINETNLTSDIILERDFFDNETKFKADEITNKNLERIPANEDYPLVIDNLTIKDRNLTMEEKDVISNSSAKKQSFTKESEVKYEVSETKFLALDKKDSCKSFPLDYSSNADIISEKFIDYPLVVGHQSIRDETLIREDKDLIADSSGKKESFTKESEIRRKVKSLNPESMSDKE-------DYPLNIDNLPTSGENLIVEDKYK-LSDSEGKKESFTKESQVKYEVSEIASLT----------------------LDKKESCKS--------FPVDYSSNVCIISEKVVDQT--------FDNKSKSVEIMERNEEALIIDSDLKFDLCQTLVTTSVEKEVSHGSMDLERYLVKDKILDEPEHLNSQQDLK----PTGFDQQRDEDNRVINTEVADETKLKFEQEALKNDQISLSIIRENKDFMSSQKEIEFETCQTVDINSIQKQESYSSFKAQIQIDAEN-----MSTQQDGQNLNHPLITDLENNENTLIQNT--DASMEIYQTPEMSSGKSKESGLYFVLEDPFMHNESKEKTEDR-------------------------NIPDDQLPKKTSEKSNESGLYFVLEDPFLLSKNTMKT-DENILPDQVSLSIVQENKDFQNSKNEIEFEACQSTDLLSNQKQESYSSFKLDHEIREDNVPEQLDDQSSLVNPL 950 The following BLAST results are available for this feature:
BLAST of SMED30004300 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004300 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004300 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30004300 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004300 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004300 ID=SMED30004300|Name=SMED30004300|organism=Schmidtea mediterranea sexual|type=transcript|length=7432bpback to top protein sequence of SMED30004300-orf-1 >SMED30004300-orf-1 ID=SMED30004300-orf-1|Name=SMED30004300-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2372bp MAWQCKHSLFMLCTRPSTRKECALDVANKLIDMVVHSYIMKSIARPLTTEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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