Nidogen 2a (osteonidogen)

Overview
NameNidogen 2a (osteonidogen)
Smed IDSMED30004230
Length (bp)637
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Nidogen 2a (osteonidogen) (SMED30004230) t-SNE clustered cells

Violin plots show distribution of expression levels for Nidogen 2a (osteonidogen) (SMED30004230) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nidogen 2a (osteonidogen) (SMED30004230) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nidogen 2a (osteonidogen) (SMED30004230) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004230

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30004230h1SMcG0020857 SmedASXL_002788SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30004230h1SMcG0020857 dd_Smed_v4_1330_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30004230h1SMcG0020857 OX_Smed_1.0.08836ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020854 Contig47817uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020854 Contig47817newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020856 Contig47817uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020856 Contig47817newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020857 Contig47817uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020857 Contig47817newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020855 Contig47817uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004230h1SMcG0020855 Contig47817newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Human
Match: NID2 (nidogen 2 [Source:HGNC Symbol;Acc:HGNC:13389])

HSP 1 Score: 55.8398 bits (133), Expect = 1.285e-8
Identity = 35/116 (30.17%), Postives = 55/116 (47.41%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRD-------EYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            +P C+E G++ P+QC GS   C CVD  G  + G  +P   ST   C  + +         E  +  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  960 IPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPP-GSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGT 1074
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Human
Match: NID2 (nidogen 2 [Source:HGNC Symbol;Acc:HGNC:13389])

HSP 1 Score: 55.0694 bits (131), Expect = 2.503e-8
Identity = 35/116 (30.17%), Postives = 55/116 (47.41%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRD-------EYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            +P C+E G++ P+QC GS   C CVD  G  + G  +P   ST   C  + +         E  +  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  554 IPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPP-GSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGT 668
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Human
Match: NID2 (nidogen 2 [Source:HGNC Symbol;Acc:HGNC:13389])

HSP 1 Score: 53.1434 bits (126), Expect = 1.090e-7
Identity = 35/116 (30.17%), Postives = 55/116 (47.41%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRD-------EYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            +P C+E G++ P+QC GS   C CVD  G  + G  +P   ST   C  + +         E  +  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  229 IPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPP-GSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGT 343
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Human
Match: SPOCK3 (SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3 [Source:HGNC Symbol;Acc:HGNC:13565])

HSP 1 Score: 47.7506 bits (112), Expect = 4.829e-6
Identity = 24/64 (37.50%), Postives = 40/64 (62.50%), Query Frame = 1 
Query:  184 CEEDEEAPDCFKKHLDV-RRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEG 366
            C + ++ P C  +  ++ +R+   K++G  +P C+E+G YKP QC GSV  C CVD++G  + G
Sbjct:  210 CFQRQQDPPCQTELSNIQKRQGVKKLLGQYIPLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVMG 273
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Human
Match: SPOCK3 (SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3 [Source:HGNC Symbol;Acc:HGNC:13565])

HSP 1 Score: 47.3654 bits (111), Expect = 6.822e-6
Identity = 24/64 (37.50%), Postives = 40/64 (62.50%), Query Frame = 1 
Query:  184 CEEDEEAPDCFKKHLDV-RRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEG 366
            C + ++ P C  +  ++ +R+   K++G  +P C+E+G YKP QC GSV  C CVD++G  + G
Sbjct:  265 CFQRQQDPPCQTELSNIQKRQGVKKLLGQYIPLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVMG 328
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Zebrafish
Match: nid2a (nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2])

HSP 1 Score: 69.707 bits (169), Expect = 2.011e-13
Identity = 47/138 (34.06%), Postives = 66/138 (47.83%), Query Frame = 1 
Query:  259 IGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCK--------CAKDRDEYQK----LRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTS------GSPTVNIGQLDQL--KCE 597
            +G  +P C+E G Y+P+QC GS   C CVD  G+   G  +P   S  + C         C + RD  Q     + ++G +   CD +G Y   QCHGS   C+C D  G++ +      GSP VN  Q   L  +CE
Sbjct:  856 VGAFIPQCDEEGQYRPLQCHGSTGHCWCVDNRGQERAGTRTPP-GSPPRNCDEPVRPKTHCEQHRDSLQSENGGVPVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQPVHLRTRCE 992

HSP 2 Score: 68.9366 bits (167), Expect = 3.023e-13
Identity = 43/129 (33.33%), Postives = 60/129 (46.51%), Query Frame = 1 
Query:  256 VIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP-------IYKSTNKTCKCAKDRDEYQK----LRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTS------GSPTVNIGQ 576
            V+G  +P C+E G Y+P QC GS   C CVD  G+   G  +P         +       C + RD  Q     + ++G +   CD +G Y   QCHGS   C+C D  G++ +      GSP VN  Q
Sbjct: 1083 VVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDSRGQERAGTRTPPGSPRVNCDQPVRSKTHCEQHRDSLQSGDGGVPVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQ 1211

HSP 3 Score: 68.5514 bits (166), Expect = 4.171e-13
Identity = 43/129 (33.33%), Postives = 61/129 (47.29%), Query Frame = 1 
Query:  256 VIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP-------IYKSTNKTCKCAKDRDEYQK----LRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTS------GSPTVNIGQ 576
            V+G  +P C+E G Y+P QC GS   C CVD  G+   G  +P         +  +   +C + RD  Q     +  +G +   CD +G Y   QCHGS   C+C D  G++ +      GSP VN  Q
Sbjct:  931 VVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQPVHLRTRCEQHRDSLQSGDGGVPAVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQ 1059

HSP 4 Score: 56.6102 bits (135), Expect = 5.287e-9
Identity = 38/123 (30.89%), Postives = 56/123 (45.53%), Query Frame = 1 
Query:  256 VIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTC---------KCAKDRDEYQKLRLIGRYFG----------CDNQGNYERTQCHG--SVCFCADENGKKTSGS 555
            V+G  +P C+E G Y+P QC GS   C CVD  G+   G  +P   S    C         +  +   E  +  L+ +Y G          CD+ G +   QC+G  S C+C D NG++  G+
Sbjct: 1159 VVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPP-GSPRVNCDQPVIVPPTQRPESVCERWRASLLQQYGGQPDSQHYLPQCDSAGEFNPVQCYGGSSYCWCVDANGREVPGT 1280
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Zebrafish
Match: nid2a (nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2])

HSP 1 Score: 63.929 bits (154), Expect = 4.737e-12
Identity = 45/136 (33.09%), Postives = 64/136 (47.06%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTC--------KCAKDRDEYQK----LRLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTS------GSPTVNIGQLDQLK 591
              +G  +P C+E G Y+P+QC GS   C CVD  G+   G  +P   S  + C         C + RD  Q     + ++G +   CD +G Y   QCHGS   C+C D  G++ +      GSP VN  Q   L+
Sbjct:   69 PAVGAFIPQCDEEGQYRPLQCHGSTGHCWCVDNRGQERAGTRTPP-GSPPRNCDEPVRPKTHCEQHRDSLQSENGGVPVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQPVHLR 203

HSP 2 Score: 50.8322 bits (120), Expect = 2.059e-7
Identity = 32/99 (32.32%), Postives = 46/99 (46.46%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIY-------KSTNKTCKCAKDRDEYQK----LRLIGRYFG-CDNQGNYERTQCHG 507
             V+G  +P C+E G Y+P QC GS   C CVD  G+   G  +P         +  +   +C + RD  Q     +  +G +   CD +G Y   QCHG
Sbjct:  145 PVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQPVHLRTRCEQHRDSLQSGDGGVPAVGAFIPQCDEEGQYRPQQCHG 243
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Zebrafish
Match: nid2a (nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2])

HSP 1 Score: 63.929 bits (154), Expect = 6.893e-12
Identity = 44/137 (32.12%), Postives = 64/137 (46.72%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIY-------KSTNKTCKCAKDRDEYQK----LRLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTS------GSPTVNIGQLDQLKCE 597
             V+G  +P C+E G Y+P QC GS   C CVD  G+   G  +P         +      +C + RD  Q     + ++G +   CD +G Y   QCHGS   C+C D  G++ +      GSP VN  Q  + K +
Sbjct:   53 PVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQPVRPKTQCEQHRDSLQSGNDGVPVVGAFIPQCDEEGQYRTQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQPVRPKTQ 189

HSP 2 Score: 59.3066 bits (142), Expect = 2.691e-10
Identity = 42/130 (32.31%), Postives = 59/130 (45.38%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIY-------KSTNKTCKCAKDRDEYQK----LRLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTS------GSPTVNIGQ 576
             V+G  +P C+E G Y+  QC GS   C CVD  G+   G  +P         +      +C + RD  Q     +  +G +   CD +G Y   QCHGS   C+C D  G++ +      GSP VN  Q
Sbjct:  129 PVVGAFIPQCDEEGQYRTQQCHGSTGHCWCVDNRGQERAGTRTPPGSPRVNCDQPVRPKTQCEQHRDSLQSGDGGVPAVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDSRGQERAGTRTPPGSPRVNCDQ 258
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Zebrafish
Match: nid2b (nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-040724-143])

HSP 1 Score: 58.151 bits (139), Expect = 1.661e-9
Identity = 38/115 (33.04%), Postives = 55/115 (47.83%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSV--CHCVDKHG-ERIEGFSSPIYKSTNKTCKCA-----KDRDEYQKLRLIGRYFG----------CDNQGNYERTQCHG--SVCFCADENGKKTSGS 555
            +P C++ G YKP+QCLGS   C CVD  G ER+   + P     N     A     +   E  +L L+  Y G          CD  GN++  QC+G  + C+C D +G +  G+
Sbjct:  745 VPKCDDKGQYKPLQCLGSTGHCWCVDSRGQERVGTRTMPGTAHANCDQPAALVPRVETVCERWRLALMSHYNGQPSSQDYMPLCDAHGNFQPVQCYGNSAFCWCVDRHGVEIIGT 859
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Zebrafish
Match: nid2a (nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2])

HSP 1 Score: 55.8398 bits (133), Expect = 5.366e-9
Identity = 38/127 (29.92%), Postives = 55/127 (43.31%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSVCHC--VDKHGERIEGFSSP------------IYKSTNKTCKCAKDRDEYQKLRLIGRYFG----------CDNQGNYERTQCHG--SVCFCADENGKKTSGS 555
             V+G  +P C+E G Y+P QC GS  HC  VD  G+   G  +P            I   T +         E  +  L+ +Y G          CD+ G +   QC+G  S C+C D NG++  G+
Sbjct:   13 PVVGAFIPQCDEEGQYRPQQCHGSTGHCWCVDSRGQERAGTRTPPGSPRVNCDQPVIVPPTQRPESVC----ERWRASLLQQYGGQPDSQHYLPQCDSAGEFNPVQCYGGSSYCWCVDANGREVPGT 135
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 64.6994 bits (156), Expect = 1.230e-11
Identity = 46/146 (31.51%), Postives = 66/146 (45.21%), Query Frame = 1 
Query:  181 ICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSPIYKSTNKTCKCAKDRD-----EYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGSPT 561
            +C E  + P C +K   +  +     +G  +P C+  G+Y P+QC GS  H  CVDK GE I G  +P  +      +    R      E  K  LI  Y G          CD  G++   QCHG+   C+C D+ G++  GS T
Sbjct: 1030 VCPEPLKTP-CLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGETEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRT 1174

HSP 2 Score: 56.225 bits (134), Expect = 1.011e-8
Identity = 48/184 (26.09%), Postives = 72/184 (39.13%), Query Frame = 1 
Query:  163 GLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSP---------------------IYKSTNKTC----------------------KCAKDRDEYQ---KLRLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            G  C +I   +     C +K   +  +     +G  +P C+  G+Y P+QC GS  H  CVDK GE I G  +P                     IY  +N T                        C + R +         +G++   CD +GNY   QCHGS   C+C D+ G++ +G+ T
Sbjct:  912 GFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGKLELVIHTHIYVFSNSTVFLKCLIVLYSPLIRVCPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRT 1095
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 63.929 bits (154), Expect = 2.500e-11
Identity = 44/150 (29.33%), Postives = 68/150 (45.33%), Query Frame = 1 
Query:  163 GLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSPIYKSTNK---------TCKCAKDRDEYQ---KLRLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            G  C +I   +     C +K   +  +     +G  +P C+  G+Y P+QC GS  H  CVDK GE I G  +P  +                C + R +         +G++   CD +GNY   QCHGS   C+C D+ G++ +G+ T
Sbjct:  912 GFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRT 1061

HSP 2 Score: 60.4622 bits (145), Expect = 3.149e-10
Identity = 45/151 (29.80%), Postives = 70/151 (46.36%), Query Frame = 1 
Query:  172 CNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHC--VDKHGERIEGFSSPIYKSTNKTCKCAKDR------DEYQKL----------RLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            C E   E  + P C +K   +  +     +G  +P C+  G+Y P+QC GS  HC  VDK GE I G  +P  +      +   +       +  Q+L            +G++   CD +GNY   QCHGS   C+C D+ G++ +G+ T
Sbjct:  993 CGERGPEPLKTP-CLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGETGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRT 1142

HSP 3 Score: 55.8398 bits (133), Expect = 1.392e-8
Identity = 46/153 (30.07%), Postives = 67/153 (43.79%), Query Frame = 1 
Query:  172 CNEICEEDEEAPDCFKKHLDVRRKLANK----VIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSPIYKSTNKTCKCAKDRD-----EYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGSPT 561
            C E   E  + P C ++   +  +L  +     +G  +P C+  G+Y  +QC GS  H  CVDK GE I G  +P  +      +    R      E  K  LI  Y G          CD  G++   QCHG+   C+C D+ G++  GS T
Sbjct: 1070 CGETGPEPLKTP-CLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPGPNCGETEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRT 1221
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 62.7734 bits (151), Expect = 4.864e-11
Identity = 48/157 (30.57%), Postives = 67/157 (42.68%), Query Frame = 1 
Query:  163 GLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSPIYKSTN----------KTCKCAKDRDEYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGSPT 561
            G  C +I   +     C +K   +  +     +G  +P C+  G+Y P+QC GS  H  CVDK GE I G  +P  +             KT  C     E  K  LI  Y G          CD  G++   QCHG+   C+C D+ G++  GS T
Sbjct:  870 GFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPLC-----ERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRT 1021
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 62.003 bits (149), Expect = 1.022e-10
Identity = 44/154 (28.57%), Postives = 70/154 (45.45%), Query Frame = 1 
Query:  163 GLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSPIYKSTNKTCKCAKDR------DEYQKL----------RLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            G  C +I   +     C +K   +  +     +G  +P C+  G+Y P+QC GS  H  CVDK GE I G  +P  +      +   +       +  Q+L            +G++   CD +GNY   QCHGS   C+C D+ G++ +G+ T
Sbjct:  912 GFQCTQILGPEPLKTPCLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGETGPEPLKTPCLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRT 1065

HSP 2 Score: 55.4546 bits (132), Expect = 1.479e-8
Identity = 46/153 (30.07%), Postives = 67/153 (43.79%), Query Frame = 1 
Query:  172 CNEICEEDEEAPDCFKKHLDVRRKLANK----VIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSPIYKSTNKTCKCAKDRD-----EYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGSPT 561
            C E   E  + P C ++   +  +L  +     +G  +P C+  G+Y  +QC GS  H  CVDK GE I G  +P  +      +    R      E  K  LI  Y G          CD  G++   QCHG+   C+C D+ G++  GS T
Sbjct:  993 CGETGPEPLKTP-CLERRQQLLGQLHPRGPRPAVGQFVPECDAEGNYVSLQCHGSTGHCWCVDKLGEEIAGTRTPPGRPGPNCGETEPTRRPQTVCERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRT 1144
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Xenopus
Match: nid2 (nidogen 2 [Source:NCBI gene;Acc:100036716])

HSP 1 Score: 61.6178 bits (148), Expect = 1.425e-10
Identity = 51/162 (31.48%), Postives = 71/162 (43.83%), Query Frame = 1 
Query:  151 KCNYGLFCNEI-CEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCH--CVDKHGERIEGFSSPIYKSTN----------KTCKCAKDRDEYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGSPT 561
            +CN G   +   C E  + P C +K   +  +     +G  +P C+  G+Y P+QC GS  H  CVDK GE I G  +P  +             KT  C     E  K  LI  Y G          CD  G++   QCHG+   C+C D+ G++  GS T
Sbjct:  874 RCNSGYEGDGFQCPEPLKTP-CLEKRQQLHPRGPRPAVGQFVPECDVEGNYVPLQCHGSTGHCWCVDKLGEEITGTRTPPGRPAPNCGERGPEPLKTPLC-----ERWKQSLIEHYGGKPGGEHYVPQCDQYGDFSPLQCHGNSGYCWCVDKEGREIEGSRT 1029
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Mouse
Match: Nid2 (nidogen 2 [Source:MGI Symbol;Acc:MGI:1298229])

HSP 1 Score: 56.225 bits (134), Expect = 6.139e-9
Identity = 33/117 (28.21%), Postives = 56/117 (47.86%), Query Frame = 1 
Query:  265 IRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP----IYKSTNKTCKCAKDRDEYQKLR--LIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            I +P C++ G++ P+QC GS   C CVD++G  + G  +P                + R   ++ R  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  986 IHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERWRESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELQGT 1102
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Mouse
Match: Nid2 (nidogen 2 [Source:MGI Symbol;Acc:MGI:1298229])

HSP 1 Score: 55.4546 bits (132), Expect = 1.345e-8
Identity = 33/117 (28.21%), Postives = 56/117 (47.86%), Query Frame = 1 
Query:  265 IRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP----IYKSTNKTCKCAKDRDEYQKLR--LIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            I +P C++ G++ P+QC GS   C CVD++G  + G  +P                + R   ++ R  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  721 IHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERWRESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELQGT 837
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Mouse
Match: Tg (thyroglobulin [Source:MGI Symbol;Acc:MGI:98733])

HSP 1 Score: 53.1434 bits (126), Expect = 7.307e-8
Identity = 33/113 (29.20%), Postives = 51/113 (45.13%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTC--KCAKDRDEYQKL---RLIGRYF---GCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQ 585
            +P+C   G Y+ +QC    C CVD  G+ ++G      +     C  KC K R + Q L   +  G  F    C  +G +   QC  S C+C D  G+   G+ +  +G+  Q
Sbjct:  617 VPSCTAGGSYEDIQCYAGECWCVDSRGKELDGSR---VRGGRPRCPTKCEKQRAQMQSLASAQPAGSSFFVPTCTREGYFLPVQCFNSECYCVDTEGQVIPGTQS-TVGEAKQ 725
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Mouse
Match: Spock3 (sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 [Source:MGI Symbol;Acc:MGI:1920152])

HSP 1 Score: 47.3654 bits (111), Expect = 5.398e-6
Identity = 24/64 (37.50%), Postives = 40/64 (62.50%), Query Frame = 1 
Query:  184 CEEDEEAPDCFKKHLDV-RRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEG 366
            C + ++ P C  +  ++ +R+   K++G  +P C+E+G YKP QC GSV  C CVD++G  + G
Sbjct:  305 CFQRQQDPPCHTELSNIQKRQGIKKLLGQYIPLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVVG 368
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Mouse
Match: Spock3 (sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 [Source:MGI Symbol;Acc:MGI:1920152])

HSP 1 Score: 47.3654 bits (111), Expect = 5.462e-6
Identity = 24/64 (37.50%), Postives = 40/64 (62.50%), Query Frame = 1 
Query:  184 CEEDEEAPDCFKKHLDV-RRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEG 366
            C + ++ P C  +  ++ +R+   K++G  +P C+E+G YKP QC GSV  C CVD++G  + G
Sbjct:  308 CFQRQQDPPCHTELSNIQKRQGIKKLLGQYIPLCDEDGYYKPTQCHGSVGQCWCVDRYGNEVVG 371
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. UniProt/SwissProt
Match: sp|P31226|SAX_LITCT (Saxiphilin OS=Lithobates catesbeianus OX=8400 PE=1 SV=3)

HSP 1 Score: 72.0182 bits (175), Expect = 2.011e-13
Identity = 48/126 (38.10%), Postives = 72/126 (57.14%), Query Frame = 1 
Query:  235 RRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTC------KCAKDR------DEYQKLRLIGRYFG-CDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            ++ LA K+IG  +P C+E G+Y+P QC GS   C CV+  GE+I G ++P  + T  TC      KC K+R      DE    +++GR+   CD +GNYE  Q HGS    +C +  G++ +G+ T
Sbjct:  115 QQALAKKMIGHYIPQCDEKGNYQPQQCHGSTGHCWCVNAMGEKISGTNTPPGQ-TRATCERHELPKCLKERQVALGGDE----KVLGRFVPQCDEKGNYEPQQFHGSTGYSWCVNAIGEEIAGTKT 235
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. UniProt/SwissProt
Match: sp|O88322|NID2_MOUSE (Nidogen-2 OS=Mus musculus OX=10090 GN=Nid2 PE=1 SV=2)

HSP 1 Score: 56.225 bits (134), Expect = 4.294e-8
Identity = 33/117 (28.21%), Postives = 56/117 (47.86%), Query Frame = 1 
Query:  265 IRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP----IYKSTNKTCKCAKDRDEYQKLR--LIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            I +P C++ G++ P+QC GS   C CVD++G  + G  +P                + R   ++ R  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  986 IHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERWRESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELQGT 1102
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. UniProt/SwissProt
Match: sp|Q14112|NID2_HUMAN (Nidogen-2 OS=Homo sapiens OX=9606 GN=NID2 PE=1 SV=3)

HSP 1 Score: 55.8398 bits (133), Expect = 6.173e-8
Identity = 35/116 (30.17%), Postives = 55/116 (47.41%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRD-------EYQKLRLIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            +P C+E G++ P+QC GS   C CVD  G  + G  +P   ST   C  + +         E  +  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  960 IPQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPP-GSTPPHCGPSPEPTQRPPTICERWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGT 1074
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. UniProt/SwissProt
Match: sp|B5DFC9|NID2_RAT (Nidogen-2 OS=Rattus norvegicus OX=10116 GN=Nid2 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 6.982e-8
Identity = 33/117 (28.21%), Postives = 56/117 (47.86%), Query Frame = 1 
Query:  265 IRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP----IYKSTNKTCKCAKDRDEYQKLR--LIGRYFG----------CDNQGNYERTQCHGS--VCFCADENGKKTSGS 555
            I +P C++ G++ P+QC GS   C CVD++G  + G  +P                + R   ++ R  L+  Y G          CD+ G++   QCHG    C+C D++G++  G+
Sbjct:  979 IHIPQCDDQGNFVPLQCHGSTGFCWCVDQNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERWRESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELPGT 1095
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. UniProt/SwissProt
Match: sp|F1RRV3|THYG_PIG (Thyroglobulin OS=Sus scrofa OX=9823 GN=TG PE=1 SV=5)

HSP 1 Score: 54.6842 bits (130), Expect = 1.518e-7
Identity = 32/105 (30.48%), Postives = 47/105 (44.76%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFG--------CDNQGNYERTQCHGSVCFCADENGKKTSGSPT 561
            +P+C   G Y+ VQC    C CVD  G  + G  +   +    T  C K R+  Q   L+GR           C  +G++   QC  S C+C D +G+   G+ T
Sbjct:  618 VPSCTAEGSYEDVQCFAGECWCVDARGRELAGSRARGGRPRCPTA-CEKQRERMQS--LLGRQPAGSSVFVPSCTREGHFLPVQCFSSDCYCVDADGQPIPGTRT 719
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. TrEMBL
Match: A0A194AKI3 (Uncharacterized protein OS=Pinctada fucata OX=50426 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 3.051e-28
Identity = 50/115 (43.48%), Postives = 78/115 (67.83%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKCE 597
             ++G   P+C+  G Y+P QC GS C+CV K+G+ I G+++ ++++   TC+CA+D+ EY    LIG++F C + G+Y+  QC G VCFCAD NG K   SP+V+I Q+ +L C+
Sbjct:  123 SLLGAPTPSCDGEGYYQPKQCQGSQCYCVSKNGKEISGYTANVWEAQQMTCQCARDQAEYMASGLIGKFFYCTSDGSYQTYQCQGDVCFCADTNGVKMDHSPSVHISQVTKLNCK 237
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. TrEMBL
Match: A0A1S3J5E7 (equistatin-like OS=Lingula unguis OX=7574 GN=LOC106170269 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 4.839e-27
Identity = 49/124 (39.52%), Postives = 74/124 (59.68%), Query Frame = 1 
Query:  223 HLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKC 594
             ++  R+    ++G   P C+ +G Y   QC  + C CV+K G+R+  +S+  +++ + TC CA+D DEYQK  LIG+ + C   GNYE+ QC GSVC+C DE G K   +P V+I  +  L C
Sbjct:    9 RIEESRRQGQPLLGATPPTCDADGTYSAKQCKEASCFCVNKDGDRLGDYSARFWEAKDMTCNCARDEDEYQKKGLIGKMYICKENGNYEKYQCTGSVCYCVDEKGSKLESTPPVSISAVKTLNC 132
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. TrEMBL
Match: A0A1S3IF36 (uncharacterized protein LOC106163748 OS=Lingula unguis OX=7574 GN=LOC106163748 PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 7.782e-27
Identity = 49/124 (39.52%), Postives = 76/124 (61.29%), Query Frame = 1 
Query:  223 HLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKC 594
             ++  R+    ++G   P C+ +G Y   QC+ + C CV+K G+R+  +S+  +++ + TC CA+D DEYQK  LIG+ + C+  GNYE+ QC GSVC+C DE G K   +P V+I  +  L C
Sbjct:  107 RIEESRRQGQPLLGATPPTCDADGTYSAKQCIEASCFCVNKDGDRLGDYSARFWEAKDMTCNCARDEDEYQKKGLIGKMYICNENGNYEKYQCTGSVCYCVDEKGSKLESTPPVSISAVKTLNC 230
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. TrEMBL
Match: A0A210PVS8 (Testican-3 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT04456 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 3.004e-24
Identity = 53/113 (46.90%), Postives = 71/113 (62.83%), Query Frame = 1 
Query:  256 VIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKC 594
            ++G   P C+ENG Y+P QC GSVC CV K G  I  + + I++S +  C+CA+D+ EY    LIGR+F C   GNY+  QC GSVC C D  G+   G+ TV+IG L +L C
Sbjct:  126 LLGAVEPLCDENGFYQPKQCEGSVCSCVSKTGRDILNYRADIWQSEDMNCQCARDQQEYMDTGLIGRFFYCTPNGNYQNHQCLGSVCHCIDNRGQMV-GTQTVSIGDLAKLSC 237
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. TrEMBL
Match: T1IZE8 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.030e-21
Identity = 48/113 (42.48%), Postives = 66/113 (58.41%), Query Frame = 1 
Query:  256 VIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKC 594
            ++GI +P C+++G Y P QC+ + CHCVDK G  I  +   + KS    CKCA+++D   +  +IGR   CD  GNYER+QC GS C+C D    +  G   V I Q D L C
Sbjct:  161 MVGIHVPKCDQDGTYSPKQCIEAYCHCVDKDGNVIVKYFFSVSKSAETECKCAREKDYLHQHGMIGRTIACDKAGNYERSQCTGSKCYCVDSKTGEKIGD-VVPISQKDSLNC 272

HSP 2 Score: 81.6481 bits (200), Expect = 5.187e-15
Identity = 42/114 (36.84%), Postives = 64/114 (56.14%), Query Frame = 1 
Query:  187 EEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCAD 528
            EE +EA D        R++   +++GI +P C+ NGDY+P +C  + C C+D  G  I G+   I KS    C+CA+ +D  +   L+G+   CD  GNY+  QC GS C+C +
Sbjct:   25 EERQEALD--------RQENNPEMVGIHVPKCDANGDYQPKECKQAYCSCLDYDGYPIRGYLFHISKSARAECRCARQKDYVRSQHLLGQIISCDKVGNYKGIQCLGSKCYCVE 130
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Cavefish
Match: nid2a (nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-030827-2])

HSP 1 Score: 70.4774 bits (171), Expect = 8.496e-14
Identity = 45/139 (32.37%), Postives = 66/139 (47.48%), Query Frame = 1 
Query:  193 DEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTC--------KCAKDRDEYQK---LRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            D     C +    ++R   +  +G  +P C+E G Y+P QC GS   C CVD  G+  +G  +P   S    C        +C + RD  Q+      +G Y   CD+QG Y + QCHGS   C+C D  G++  G+ T
Sbjct:  917 DRPKTQCEQHRDSLQRGDGSPPVGAFVPECDEQGQYRPQQCHGSTGHCWCVDIRGQERQGTRTPP-GSPQFNCDAPERPKTQCEQHRDSLQRGDGSPPVGAYVPQCDDQGQYRQQQCHGSTGHCWCVDSRGQERQGTRT 1054

HSP 2 Score: 70.4774 bits (171), Expect = 9.165e-14
Identity = 44/139 (31.65%), Postives = 67/139 (48.20%), Query Frame = 1 
Query:  184 CEEDEEAPDCFKKHLD-VRRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGF----SSPIYKSTNKTCKCAKDRDEYQK---LRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            C+  E      ++H D ++R   +  +G  +P C++ G Y+P QC GS   C CVD  G+  +G      SP +       +C + RD  Q+      +G +   CD QG Y   QCHGS   C+C D  G++  G+ T
Sbjct:  841 CDAPERPKTQCEQHRDSLQRGDGSPPVGAYVPQCDDQGQYRPQQCHGSTGHCWCVDSRGQERQGTRTPPGSPRFNCDRPKTQCEQHRDSLQRGDGSPPVGAFVPECDEQGQYRPQQCHGSTGHCWCVDIRGQERQGTRT 979

HSP 3 Score: 67.781 bits (164), Expect = 7.432e-13
Identity = 42/116 (36.21%), Postives = 57/116 (49.14%), Query Frame = 1 
Query:  262 GIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTC--------KCAKDRDEYQK---LRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTSGSPT 561
            G  +P C+E G Y+P QC GS   C CVD  G+  +G  +P   S    C        +C + RD  Q+      +G Y   CD+QG Y   QCHGS   C+C D  G++  G+ T
Sbjct:  793 GAFVPECDEQGQYRPQQCHGSTGHCWCVDSRGQERQGTRTPP-GSPQFNCDAPERPKTQCEQHRDSLQRGDGSPPVGAYVPQCDDQGQYRPQQCHGSTGHCWCVDSRGQERQGTRT 907

HSP 4 Score: 59.6918 bits (143), Expect = 3.681e-10
Identity = 41/145 (28.28%), Postives = 66/145 (45.52%), Query Frame = 1 
Query:  184 CEEDEEAPDCFKKHLD-VRRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP----IYKSTNKTCKCAKDRDEYQKLR--LIGRYFG----------CDNQGNYERTQCHG--SVCFCADENGKKTSGS 555
            C+  E      ++H D ++R   +  IG  +P C+E G Y+P QC GS   C CVD  G+  +G  +P     Y          +     ++ R  L+  Y G          CD+ G +   QC+G  S C+C D +G++ +G+
Sbjct: 1063 CDAPERPKTQCEQHRDSLQRGDGSPPIGAYVPQCDEQGQYRPQQCHGSTGHCWCVDSRGQERQGTRTPPGTSPYNCDAPVVPTQRPETVCERWRASLLEHYGGRPSPQQYLPQCDSAGEFNPVQCYGDSSYCWCVDRDGREVAGT 1207
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Cavefish
Match: tg (thyroglobulin [Source:NCBI gene;Acc:103037495])

HSP 1 Score: 59.3066 bits (142), Expect = 5.754e-10
Identity = 36/114 (31.58%), Postives = 51/114 (44.74%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFG------CDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKC 594
            LP C E+G Y+ VQC GS C C D  G+ + G  S  Y+    + +C K+R     ++             C+  G Y   QC G  CFC D+ G + S      +G    L+C
Sbjct:  626 LPQCTEDGQYQAVQCQGSECWCADSQGQEVMGSRSIGYRPRCPS-RCEKERMMAIAVKASSSAGSEVFIPKCEEDGEYVSLQCLGKSCFCLDQRGVRYS-----TLGSGSSLQC 733
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Cavefish
Match: nid2b (nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GENE-040724-143])

HSP 1 Score: 58.9214 bits (141), Expect = 7.130e-10
Identity = 38/114 (33.33%), Postives = 54/114 (47.37%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSV--CHCVDKHG-ERIEGFSSPIYKSTN----KTCKCAKDRDEYQKLRLIGRYFG----------CDNQGNYERTQCHG--SVCFCADENGKKTSGS 555
            +P C+E G YKP+QCLGS   C CVD  G ER+   + P     N         A+   E  +  L+  Y G          CD +G++   QC+G  S C+C D  G++  G+
Sbjct:  721 IPQCDEKGQYKPLQCLGSTGHCWCVDSRGQERVGTRTLPGTAHANCDQPPLVPRAETLCERWRTTLLSHYGGQPDPQDYMPQCDARGHFLSVQCYGNSSYCWCVDSQGREVMGT 834
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Sea Lamprey
Match: tg (thyroglobulin [Source:ZFIN;Acc:ZDB-GENE-030519-1])

HSP 1 Score: 59.3066 bits (142), Expect = 1.128e-10
Identity = 34/98 (34.69%), Postives = 42/98 (42.86%), Query Frame = 1 
Query:  283 EENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFG-------CDNQGNYERTQCHGSVCFCADENGKKTSGS 555
            E+ G Y+PVQC G+ C CVD HG  I G  +           C  +R      R  GR  G       CD  G+Y   QC G  CFC    G +  G+
Sbjct:  612 EDGGAYEPVQCHGAACWCVDAHGREIAGTRAVGGAPPRCPSACEGERARALLARR-GRPVGTPLFVPECDGAGDYRPVQCSGDRCFCVGAGGAEVPGT 708

HSP 2 Score: 49.6766 bits (117), Expect = 2.007e-7
Identity = 28/96 (29.17%), Postives = 40/96 (41.67%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTN--KTCKCAKDRDEYQKLRLIGRYF--------------GCDNQGNYERTQCHGS 510
            +P C+  GDY+PVQC G  C CV   G  + G   P+         C+ A   +  Q+LR +G                  CD +G +   QC G+
Sbjct:  676 VPECDGAGDYRPVQCSGDRCFCVGAGGAEVPGTGRPLGDPVTCPTPCQAAAASELLQQLRHLGPVLRGDAPSSLPPLYVPRCDARGGWRPVQCDGA 771
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Nematostella
Match: EDO39095 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB04])

HSP 1 Score: 62.3882 bits (150), Expect = 2.067e-12
Identity = 41/116 (35.34%), Postives = 60/116 (51.72%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGF---------SSPIYKSTN-KTCKCAKDRDEYQKLRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTSGS 555
             V+G  +P C   G+YK VQC GS   C CVD+ G  + G          SS +   TN +T + A  +     L+ +GR+   CD +G YE+ QC GS+  C+C D +G +  G+
Sbjct:    2 PVVGKYIPQCTPEGEYKQVQCYGSTGYCWCVDQMGSPVLGTIVRGVPQCNSSAVGGMTNCQTKRAAALQGSQGGLQPVGRFLPSCDVKGAYEKIQCWGSIGFCWCVDSSGNEIKGT 117

HSP 2 Score: 54.6842 bits (130), Expect = 1.231e-9
Identity = 34/93 (36.56%), Postives = 45/93 (48.39%), Query Frame = 1 
Query:  259 IGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLR--LIGRYFG-CDNQGNYERTQCHGSVCFC 522
            +G  LP+C+  G Y+ +QC GS+  C CVD  G  I+G       S + T         + + R  LIG Y   CD  G Y   QCHGS  +C
Sbjct:   79 VGRFLPSCDVKGAYEKIQCWGSIGFCWCVDSSGNEIKGTRVRGTPSCDTTPAPTASGKHWAQSRGPLIGAYIPQCDKDGRYSALQCHGSTGYC 171
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Nematostella
Match: EDO26317 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TCB6])

HSP 1 Score: 54.299 bits (129), Expect = 2.975e-9
Identity = 37/113 (32.74%), Postives = 57/113 (50.44%), Query Frame = 1 
Query:  253 KVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGF---------SSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFG-CDNQGNYERTQCHGSVCFCADENGKKTSGS 555
            + +G  LP+C+  G Y+ VQC GS+  C CVD  G  I+G          ++P   ++  T  C   R  +Q   L+G +   CDN G YE+ Q H    +C D  G++ +G+
Sbjct:   20 QPVGRFLPSCDVKGAYEKVQCWGSIGFCWCVDSSGNEIKGTRVRGTPSCDTTPAPTASGLT-DCQLRR--HQAAGLLGAFRPLCDNAGAYEKVQSHEGYYWCVDSQGREINGT 129

HSP 2 Score: 50.0618 bits (118), Expect = 7.517e-8
Identity = 32/98 (32.65%), Postives = 46/98 (46.94%), Query Frame = 1 
Query:  232 VRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFG-CDNQGNYERTQCHGSVCFC 522
            +RR  A  ++G   P C+  G Y+ VQ       CVD  G  I G      K TN    C  + +   ++ ++GRY   CD  G++ + QCH S  FC
Sbjct:   84 LRRHQAAGLLGAFRPLCDNAGAYEKVQSHEGYYWCVDSQGREINGTRLRFNKPTN----CTSNSNSGPRM-MVGRYVPQCDKDGSFHQVQCHPSTGFC 176
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Nematostella
Match: EDO40372 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7C3])

HSP 1 Score: 53.1434 bits (126), Expect = 6.670e-9
Identity = 37/107 (34.58%), Postives = 53/107 (49.53%), Query Frame = 1 
Query:  271 LPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFS----SPIYKSTNKTCKCAKDRDE---YQKLRLIGRYFG-CDNQGNYERTQCHG--SVCFCADENGKKTSGS 555
            +P C+ +G Y+PVQCL +   C CVDK G  I G       P+ K    T  C ++R     +  LR+ G +   C   G+YE  QCH     C+C D +G +  G+
Sbjct:   75 VPRCDASGGYEPVQCLSTTGHCWCVDKDGIEIRGTRRRGVRPVCKQGYNT-PCERERYRALGWTGLRVTGLWVPDCRPDGSYESVQCHALSGYCWCVDRSGHELFGT 180
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Nematostella
Match: EDO40373 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7C4])

HSP 1 Score: 52.7582 bits (125), Expect = 1.007e-8
Identity = 38/118 (32.20%), Postives = 56/118 (47.46%), Query Frame = 1 
Query:  238 RKLANKVIGIR-----LPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGF---SSPIYKSTNKTCKCAKDRDEYQKLRLI---GRYF-GCDNQGNYERTQCHGSV--CFCADENGKK 543
            R++A    G+      +P C+ +GDY  VQC GS   C CVD  G  I        P+        +C ++R  +  +  I   GR+   C + G YER QCH S   C+C D+ G++
Sbjct:   62 RQIAIGWTGVAAPGSFVPKCKSSGDYDTVQCHGSTGFCWCVDDDGNEIPATRVRGRPMCGLLAGLSQCQQERQRHMGVLGIAPPGRFVPACTSTGQYERVQCHSSTGYCWCVDKFGRE 179

HSP 2 Score: 50.447 bits (119), Expect = 5.303e-8
Identity = 33/106 (31.13%), Postives = 53/106 (50.00%), Query Frame = 1 
Query:  256 VIGIRLPNCEENGDYKPVQC--LGSVCHCVDKHGERIEGFSSPIYKSTNKT----CKCAKDRD---EYQKLRLIGRYFG-CDNQGNYERTQCHGSV--CFCADENG 537
            V G+ +P+C  +G Y+ VQC  L   C CVD+ G  + G      ++ +K       C K+R     +  +   G +   C + G+Y+  QCHGS   C+C D++G
Sbjct:    1 VTGLWVPDCRPDGSYESVQCHALSGYCWCVDRSGHELFGTRVRGKRTCDKIKAGLTMCQKERQIAIGWTGVAAPGSFVPKCKSSGDYDTVQCHGSTGFCWCVDDDG 106
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Nematostella
Match: EDO40365 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7B6])

HSP 1 Score: 50.8322 bits (120), Expect = 5.592e-8
Identity = 37/125 (29.60%), Postives = 56/125 (44.80%), Query Frame = 1 
Query:  223 HLDVRRKLANK---VIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGF---SSPIYKSTNKTCKCAKDRDE---YQKLRLIGRYFG-CDNQGNYERTQCH--GSVCFCADENGKKTSGS 555
            H D  R LA     +I   +P C  +G ++ +QC  +   C CVD +G  + G    S P+  S     +C ++R     +  + + G +   C   G YER QCH     C+C D NG +   S
Sbjct:   83 HTDRMRALAWTGRLIINTFVPRCLPDGSFEAIQCQPATGKCWCVDVNGNELVGTRTDSKPVCTSRAGLSECQRERQRVLGWSGVAVDGTFVPECTADGGYERVQCHEVTGFCWCVDGNGNEIPKS 207
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Medaka
Match: nid2a (nidogen 2 [Source:NCBI gene;Acc:101173773])

HSP 1 Score: 57.3806 bits (137), Expect = 2.618e-9
Identity = 39/132 (29.55%), Postives = 59/132 (44.70%), Query Frame = 1 
Query:  217 KKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLR------------LIGRYF-GCDNQGNYERTQCHGS--VCFCADENGKKTSGSPT 561
            ++H+    +     +G  +P C++NG+Y P QC  S   C CVD  G+   G  S     +    K    +   ++LR            L G Y   CD  G+Y   QCHGS   C+C D +G++  G+ T
Sbjct:  847 REHVLASEREDRPSVGAYVPQCDQNGEYVPQQCHTSTGYCWCVDGMGQERSGTRSAPGSPSMDCSKPGAPKTPCERLRESVLASSAGGRPLAGAYAPQCDEDGHYVPRQCHGSSGYCWCVDSSGQERPGTRT 978

HSP 2 Score: 55.4546 bits (132), Expect = 9.526e-9
Identity = 36/117 (30.77%), Postives = 55/117 (47.01%), Query Frame = 1 
Query:  256 VIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP-------IYKSTNKTCKCAKDRD-----EYQKLRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTSGS 555
            ++G+ +P C+E+G Y P QC  S   C CVD  G+   G  +P         +  +   +C + R+     E +    +G Y   CD  G Y   QCH S   C+C D  G++ SG+
Sbjct:  783 LLGVYVPQCDESGKYLPQQCHDSTGYCWCVDSTGQERTGTRTPPGSALVDCNEQPHYKTRCERHREHVLASEREDRPSVGAYVPQCDQNGEYVPQQCHTSTGYCWCVDGMGQERSGT 899

HSP 3 Score: 55.4546 bits (132), Expect = 9.982e-9
Identity = 42/125 (33.60%), Postives = 59/125 (47.20%), Query Frame = 1 
Query:  262 GIRLPNCEENGDYKPVQCLGS--VCHCVDKHG-ERIEGFSSPIYKSTN--------KTCKCAKDR---DEYQKLRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTSGSPT-VNIGQLD 582
            G   P C+E+G Y P QC GS   C CVD  G ER    +SP   S +          C+  ++R      + +   GR+   CD +G Y   QCHGS   C+C D  G++  G+ T   + Q+D
Sbjct:  939 GAYAPQCDEDGHYVPRQCHGSSGYCWCVDSSGQERPGTRTSPGAPSVDCKEPEAPKTECELLRERAMASSPEAVPAAGRFVPQCDAEGRYLSQQCHGSTGHCWCVDSRGQERPGTRTPPGVAQVD 1063
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Medaka
Match: nid2a (nidogen 2 [Source:NCBI gene;Acc:101173773])

HSP 1 Score: 56.6102 bits (135), Expect = 3.681e-9
Identity = 50/182 (27.47%), Postives = 78/182 (42.86%), Query Frame = 1 
Query:  133 CLNEEDKCNYGLFCNEICEEDEEA---PDCFKKHLDVRRKLAN--KVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSP----------IYKSTNKTCKCAKDRD-----EYQKLRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTS------GSPTVNIGQLDQ 585
            CL +      G FC++  +ED  A     C      V+R       ++G+ +P C+E+G Y P QC  S   C CVD  G+   G  +P            +  +   +C + R+     E +    +G Y   CD  G Y   QCH S   C+C D  G++ S      GSP+++  + D+
Sbjct:  815 CLCQPGFTGDGFFCSQ--QEDLPARPKTRCEHHRESVQRTTEEGAPLLGVYVPQCDESGKYLPQQCHDSTGYCWCVDSTGQERTGTRTPPGSALVDCSRTDEQPHYKTRCERHREHVLASEREDRPSVGAYVPQCDQNGEYVPQQCHTSTGYCWCVDGMGQERSGTRSAPGSPSMDCSKPDE 994

HSP 2 Score: 55.8398 bits (133), Expect = 7.638e-9
Identity = 44/155 (28.39%), Postives = 67/155 (43.23%), Query Frame = 1 
Query:  184 CEEDEEAP------DCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSV--CHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQ------KLR------------LIGRYF-GCDNQGNYERTQCHGS--VCFCADENGKKTSGSPT 561
            C   +E P      +  ++H+    +     +G  +P C++NG+Y P QC  S   C CVD  G+   G  S      + +  C+K  +  Q      +LR            L G Y   CD  G+Y   QCHGS   C+C D +G++  G+ T
Sbjct:  909 CSRTDEQPHYKTRCERHREHVLASEREDRPSVGAYVPQCDQNGEYVPQQCHTSTGYCWCVDGMGQERSGTRS---APGSPSMDCSKPDEALQPKTPCERLRESVLASSAGGRPLAGAYAPQCDEDGHYVPRQCHGSSGYCWCVDSSGQERPGTRT 1060

HSP 3 Score: 55.0694 bits (131), Expect = 1.208e-8
Identity = 42/128 (32.81%), Postives = 59/128 (46.09%), Query Frame = 1 
Query:  262 GIRLPNCEENGDYKPVQCLGS--VCHCVDKHG-ERIEGFSSPIYKSTN-----------KTCKCAKDR---DEYQKLRLIGRYF-GCDNQGNYERTQCHGSV--CFCADENGKKTSGSPT-VNIGQLD 582
            G   P C+E+G Y P QC GS   C CVD  G ER    +SP   S +             C+  ++R      + +   GR+   CD +G Y   QCHGS   C+C D  G++  G+ T   + Q+D
Sbjct: 1021 GAYAPQCDEDGHYVPRQCHGSSGYCWCVDSSGQERPGTRTSPGAPSVDCSRAEEPEAPKTECELLRERAMASSPEAVPAAGRFVPQCDAEGRYLSQQCHGSTGHCWCVDSRGQERPGTRTPPGVAQVD 1148
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Planmine SMEST
Match: SMESG000016994.1 (SMESG000016994.1)

HSP 1 Score: 357.451 bits (916), Expect = 1.339e-127
Identity = 175/179 (97.77%), Postives = 176/179 (98.32%), Query Frame = 1 
Query:   61 MLKIFVISLLTSCVFCEVLEESQLCLNEEDKCNYGLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKCE 597
            MLKIFVISL  SCVFCEVLEE QLCLNEEDKCNYGLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNY+RTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKCE
Sbjct:    1 MLKIFVISLFASCVFCEVLEEFQLCLNEEDKCNYGLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYKRTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKCE 179
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Planmine SMEST
Match: SMESG000016993.1 (SMESG000016993.1)

HSP 1 Score: 231.876 bits (590), Expect = 5.872e-78
Identity = 109/179 (60.89%), Postives = 138/179 (77.09%), Query Frame = 1 
Query:   61 MLKIFVISLLTSCVFCEVLEESQLCLNEEDKCNYGLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKCE 597
            MLKIFV   L   +F  +LEE ++C N E+ CNYGL C+++C+ED EAPDCFK+HL VRR+    ++G R P+CEENGDYK +QC+GSVC+C DK+G  I GF +PI+ S NK CKCAK+RDEY K  LIGRYFGC++ GNYE+ QCHGSVC+CADE+GKK  GS TV+I   D+LKC+
Sbjct:    1 MLKIFVAFSLFCGIFSALLEEFEVCENNENVCNYGLSCDKVCKEDTEAPDCFKQHLSVRRRWKEHILGARAPSCEENGDYKALQCVGSVCYCSDKNGVEISGFITPIHLSANKNCKCAKERDEYLKSHLIGRYFGCNHLGNYEKAQCHGSVCYCADEDGKKVEGSQTVHIVDFDKLKCD 179
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Planmine SMEST
Match: SMESG000016992.1 (SMESG000016992.1)

HSP 1 Score: 226.868 bits (577), Expect = 4.922e-76
Identity = 107/179 (59.78%), Postives = 135/179 (75.42%), Query Frame = 1 
Query:   61 MLKIFVISLLTSCVFCEVLEESQLCLNEEDKCNYGLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKCE 597
            MLKIFV   L   +F  +LEE ++C N E+ CNYGL C+++C+ED EAPDCFK+HL VRR+    ++G R P+CEENGDYK +QC+GSVC+C DK+G  I GF +PI+ S NK CKCAK+RDEY K  LIGR+F C   GNYE+ QCHGSVC+CADE+GKK  GS TV+I   D+LKC+
Sbjct:    1 MLKIFVAFSLFCGIFSALLEEFEVCENNENVCNYGLSCDKVCKEDTEAPDCFKQHLAVRRRWKEHILGARAPSCEENGDYKALQCVGSVCYCSDKNGVEISGFITPIHLSANKNCKCAKERDEYHKKHLIGRFFRCSELGNYEKAQCHGSVCYCADEDGKKVEGSQTVHIVDFDKLKCD 179
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Planmine SMEST
Match: SMESG000016991.1 (SMESG000016991.1)

HSP 1 Score: 139.428 bits (350), Expect = 4.198e-42
Identity = 65/116 (56.03%), Postives = 85/116 (73.28%), Query Frame = 1 
Query:   61 MLKIFVISLLTSCVFCEVLEESQLCLNEEDKCNYGLFCNEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCK 408
            MLKIFV   L   +F  +LEE ++C N E+ CNYGL C+++C+ED EAPDCFK+HL VRR+    ++G R P+CEENGDYK +QC+GSVC+C DK+G  I GF +PI+ S NK C 
Sbjct:    1 MLKIFVAFSLFCGIFSALLEEFEVCENNENVCNYGLSCDKVCKEDTEAPDCFKQHLAVRRRWKEHILGARAPSCEENGDYKALQCVGSVCYCSDKNGVEISGFITPIHLSANKNCS 116
[Close]
BLAST of Nidogen 2a (osteonidogen) vs. Planmine SMEST
Match: SMESG000016977.1 (SMESG000016977.1)

HSP 1 Score: 131.724 bits (330), Expect = 1.175e-38
Identity = 70/176 (39.77%), Postives = 101/176 (57.39%), Query Frame = 1 
Query:   76 VISLLTSCVFCEVLEESQLCLNEEDKCNYGLFC--NEICEEDEEAPDCFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERIEGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGSVCFCADENGKKTSGSPTVNIGQLDQLKCE 597
            ++SL+T      + +    C +  D CN  L C  NE C ++     CF K    R+     ++G  +P C+ NGD+ PVQC GS C+CVD+ G  I  +++ I +  N  CKCA+++++Y    LIG+ F C   G+Y   QC+GSVCFCAD NGK  +G P VNIGQ+  L+C 
Sbjct:    5 ILSLITIMNLLLINKVYGTCQDSSD-CNNDLICIGNE-CSDNPLNTQCFNKLSKARKG---GLLGAPIPKCDSNGDFLPVQCTGSKCYCVDREGNNIRNYAAHINEFANMDCKCAREQNDYFLTGLIGKMFSCTTNGSYSSVQCNGSVCFCADYNGKAVAGKPIVNIGQIQSLRCN 175
The following BLAST results are available for this feature:
BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NID21.285e-830.17nidogen 2 [Source:HGNC Symbol;Acc:HGNC:13389][more]
NID22.503e-830.17nidogen 2 [Source:HGNC Symbol;Acc:HGNC:13389][more]
NID21.090e-730.17nidogen 2 [Source:HGNC Symbol;Acc:HGNC:13389][more]
SPOCK34.829e-637.50SPARC (osteonectin), cwcv and kazal like domains p... [more]
SPOCK36.822e-637.50SPARC (osteonectin), cwcv and kazal like domains p... [more]
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nid2a2.011e-1334.06nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
nid2a4.737e-1233.09nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
nid2a6.893e-1232.12nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
nid2b1.661e-933.04nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
nid2a5.366e-929.92nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nid21.230e-1131.51nidogen 2 [Source:NCBI gene;Acc:100036716][more]
nid22.500e-1129.33nidogen 2 [Source:NCBI gene;Acc:100036716][more]
nid24.864e-1130.57nidogen 2 [Source:NCBI gene;Acc:100036716][more]
nid21.022e-1028.57nidogen 2 [Source:NCBI gene;Acc:100036716][more]
nid21.425e-1031.48nidogen 2 [Source:NCBI gene;Acc:100036716][more]
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nid26.139e-928.21nidogen 2 [Source:MGI Symbol;Acc:MGI:1298229][more]
Nid21.345e-828.21nidogen 2 [Source:MGI Symbol;Acc:MGI:1298229][more]
Tg7.307e-829.20thyroglobulin [Source:MGI Symbol;Acc:MGI:98733][more]
Spock35.398e-637.50sparc/osteonectin, cwcv and kazal-like domains pro... [more]
Spock35.462e-637.50sparc/osteonectin, cwcv and kazal-like domains pro... [more]
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BLAST of Nidogen 2a (osteonidogen) vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P31226|SAX_LITCT2.011e-1338.10Saxiphilin OS=Lithobates catesbeianus OX=8400 PE=1... [more]
sp|O88322|NID2_MOUSE4.294e-828.21Nidogen-2 OS=Mus musculus OX=10090 GN=Nid2 PE=1 SV... [more]
sp|Q14112|NID2_HUMAN6.173e-830.17Nidogen-2 OS=Homo sapiens OX=9606 GN=NID2 PE=1 SV=... [more]
sp|B5DFC9|NID2_RAT6.982e-828.21Nidogen-2 OS=Rattus norvegicus OX=10116 GN=Nid2 PE... [more]
sp|F1RRV3|THYG_PIG1.518e-730.48Thyroglobulin OS=Sus scrofa OX=9823 GN=TG PE=1 SV=... [more]
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BLAST of Nidogen 2a (osteonidogen) vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A194AKI33.051e-2843.48Uncharacterized protein OS=Pinctada fucata OX=5042... [more]
A0A1S3J5E74.839e-2739.52equistatin-like OS=Lingula unguis OX=7574 GN=LOC10... [more]
A0A1S3IF367.782e-2739.52uncharacterized protein LOC106163748 OS=Lingula un... [more]
A0A210PVS83.004e-2446.90Testican-3 OS=Mizuhopecten yessoensis OX=6573 GN=K... [more]
T1IZE81.030e-2142.48Uncharacterized protein OS=Strigamia maritima OX=1... [more]
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nid2a8.496e-1432.37nidogen 2a (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
tg5.754e-1031.58thyroglobulin [Source:NCBI gene;Acc:103037495][more]
nid2b7.130e-1033.33nidogen 2b (osteonidogen) [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tg1.128e-1034.69thyroglobulin [Source:ZFIN;Acc:ZDB-GENE-030519-1][more]
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO390952.067e-1235.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO263172.975e-932.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO403726.670e-934.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO403731.007e-832.20Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO403655.592e-829.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Nidogen 2a (osteonidogen) vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
nid2a2.618e-929.55nidogen 2 [Source:NCBI gene;Acc:101173773][more]
nid2a3.681e-927.47nidogen 2 [Source:NCBI gene;Acc:101173773][more]
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BLAST of Nidogen 2a (osteonidogen) vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000016994.11.339e-12797.77SMESG000016994.1[more]
SMESG000016993.15.872e-7860.89SMESG000016993.1[more]
SMESG000016992.14.922e-7659.78SMESG000016992.1[more]
SMESG000016991.14.198e-4256.03SMESG000016991.1[more]
SMESG000016977.11.175e-3839.77SMESG000016977.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004230 ID=SMED30004230|Name=Nidogen 2a (osteonidogen)|organism=Schmidtea mediterranea sexual|type=transcript|length=637bp
CTCACATTCATTCAAAAGGAATTTTTTATTTGTTTCCGATTTTTTGTCTT
TTAGCTAAAAATGTTAAAGATTTTTGTAATTTCACTATTAACGTCTTGCG
TTTTCTGTGAGGTCCTGGAGGAATCCCAGCTGTGTTTGAATGAAGAGGAC
AAATGTAATTACGGGCTATTTTGCAATGAAATTTGTGAGGAAGACGAGGA
AGCACCGGACTGCTTTAAAAAACATCTAGATGTCCGACGAAAATTGGCTA
ACAAAGTGATAGGAATTCGACTCCCCAACTGTGAGGAAAATGGTGACTAC
AAACCCGTTCAATGTCTCGGCTCCGTGTGTCATTGCGTTGACAAGCATGG
AGAGCGGATTGAAGGATTTTCCTCTCCAATTTATAAATCTACTAACAAAA
CTTGCAAATGCGCAAAAGATCGTGATGAATATCAAAAACTCCGTTTAATT
GGGAGATATTTCGGTTGTGACAACCAAGGAAATTACGAAAGGACCCAATG
CCACGGTTCAGTGTGTTTCTGTGCCGATGAGAATGGAAAGAAAACTAGCG
GGTCACCAACTGTCAATATTGGCCAATTAGATCAATTAAAATGTGAATAA
TTCAATCTACAAATAAATTTGCGACTTTAAAAAAAAA
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protein sequence of SMED30004230-orf-1

>SMED30004230-orf-1 ID=SMED30004230-orf-1|Name=SMED30004230-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=180bp
MLKIFVISLLTSCVFCEVLEESQLCLNEEDKCNYGLFCNEICEEDEEAPD
CFKKHLDVRRKLANKVIGIRLPNCEENGDYKPVQCLGSVCHCVDKHGERI
EGFSSPIYKSTNKTCKCAKDRDEYQKLRLIGRYFGCDNQGNYERTQCHGS
VCFCADENGKKTSGSPTVNIGQLDQLKCE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0005179hormone activity
Vocabulary: biological process
TermDefinition
GO:0007160cell-matrix adhesion
GO:0031101fin regeneration
GO:0015705iodide transport
GO:0030878thyroid gland development
GO:0031641regulation of myelination
GO:0042403thyroid hormone metabolic process
GO:0042446hormone biosynthetic process
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR036857Thyroglobulin_1_sf
IPR000716Thyroglobulin_1
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
GO:0005615extracellular space
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000716Thyroglobulin type-1SMARTSM00211TY_2coord: 133..174
e-value: 0.0012
score: 28.1
coord: 71..121
e-value: 1.7E-8
score: 44.2
IPR000716Thyroglobulin type-1PFAMPF00086Thyroglobulin_1coord: 136..169
e-value: 6.7E-9
score: 35.3
coord: 63..104
e-value: 1.5E-12
score: 47.0
IPR000716Thyroglobulin type-1PROSITEPS51162THYROGLOBULIN_1_2coord: 48..115
score: 9.308
IPR000716Thyroglobulin type-1PROSITEPS51162THYROGLOBULIN_1_2coord: 116..178
score: 7.408
IPR000716Thyroglobulin type-1CDDcd00191TYcoord: 59..102
e-value: 6.47196E-10
score: 50.9253
IPR000716Thyroglobulin type-1CDDcd00191TYcoord: 136..169
e-value: 1.28437E-6
score: 42.0657
IPR036857Thyroglobulin type-1 superfamilyGENE3DG3DSA:4.10.800.10coord: 116..178
e-value: 1.2E-8
score: 36.2
coord: 50..115
e-value: 7.8E-11
score: 43.2
IPR036857Thyroglobulin type-1 superfamilySUPERFAMILYSSF57610Thyroglobulin type-1 domaincoord: 135..171
IPR036857Thyroglobulin type-1 superfamilySUPERFAMILYSSF57610Thyroglobulin type-1 domaincoord: 33..105
NoneNo IPR availablePANTHERPTHR12352SECRETED MODULAR CALCIUM-BINDING PROTEINcoord: 23..103
coord: 111..168
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.844