Sodium/hydrogen exchanger 11

Overview
NameSodium/hydrogen exchanger 11
Smed IDSMED30004224
Length (bp)777
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Sodium/hydrogen exchanger 11 (SMED30004224) t-SNE clustered cells

Violin plots show distribution of expression levels for Sodium/hydrogen exchanger 11 (SMED30004224) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sodium/hydrogen exchanger 11 (SMED30004224) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sodium/hydrogen exchanger 11 (SMED30004224) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004224

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30004224h1SMcG0015393 Contig2193GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30004224h1SMcG0015393 dd_Smed_v4_10212_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30004224h1SMcG0015393 dd_Smed_v4_10212_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30004224h1SMcG0015393 dd_Smed_v6_10212_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30004224h1SMcG0015393 dd_Smed_v4_10212_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Human
Match: AL139142.2 (novel protein)

HSP 1 Score: 75.8702 bits (185), Expect = 1.112e-15
Identity = 48/177 (27.12%), Postives = 85/177 (48.02%), Query Frame = 3 
Query:  162 LDELLSSKNLFNQTPIDYSIILA-RFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMN 689
            L+E +  +NL       Y+  +A + D+ + L       NE    GY+ +H A  W RL+ LK + +L  D+     R E    ++ RY   +   +L W +AR +L  Y+      V   EK  G+L K+DK    S C  +  WL+++ +A+I +   + ++ + ++  I+ KM 
Sbjct:   71 LEEDIVGRNLL------YAACMAGQSDVIRALAKYGVNLNEKTTRGYTLLHCAAAWGRLETLKALVELDVDIEALNFREERARDVAARYSQTECVEFLDWADARLTLKKYIAKVSLAVTDTEKGSGKLLKEDKNTILSACRAKNEWLETHTEASINELFEQRQQLEDIVTPIFTKMT 241
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Human
Match: ANKRD45 (ankyrin repeat domain 45 [Source:HGNC Symbol;Acc:HGNC:24786])

HSP 1 Score: 75.8702 bits (185), Expect = 1.112e-15
Identity = 48/177 (27.12%), Postives = 85/177 (48.02%), Query Frame = 3 
Query:  162 LDELLSSKNLFNQTPIDYSIILA-RFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMN 689
            L+E +  +NL       Y+  +A + D+ + L       NE    GY+ +H A  W RL+ LK + +L  D+     R E    ++ RY   +   +L W +AR +L  Y+      V   EK  G+L K+DK    S C  +  WL+++ +A+I +   + ++ + ++  I+ KM 
Sbjct:   71 LEEDIVGRNLL------YAACMAGQSDVIRALAKYGVNLNEKTTRGYTLLHCAAAWGRLETLKALVELDVDIEALNFREERARDVAARYSQTECVEFLDWADARLTLKKYIAKVSLAVTDTEKGSGKLLKEDKNTILSACRAKNEWLETHTEASINELFEQRQQLEDIVTPIFTKMT 241
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Zebrafish
Match: ankrd45 (ankyrin repeat domain 45 [Source:ZFIN;Acc:ZDB-GENE-050522-311])

HSP 1 Score: 88.5817 bits (218), Expect = 9.825e-21
Identity = 50/194 (25.77%), Postives = 95/194 (48.97%), Query Frame = 3 
Query:  141 SLISREYLDELLSSKNLF------NQTPIDYSIILARFDIFKVLI-SKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMNDNPD 701
             ++ R + D+   S+N+        +  +  + ++ R  I + L+ +     NE+   GYS +H + +W +LD LK + +L  D      R E  + ++ RY   D   YL W  A+ +L  ++ + R +V   EK QG+L K+DK +C + C  +  W+ +   A  +DF  + +  + +L  +  K+N  P+
Sbjct:   23 GILERTFTDDAAQSENILWEKDEVGRNALFAACMMGRSAIVRELVQNGAADVNELTARGYSPLHCSAMWGQLDTLKTLVELNADFQAINFRGEKAVDVARRYDKLDCAEYLAWAEAKQNLQAFIQEVRAIVADQEKVQGKLNKEDKNICINTCSAKSDWINNTRTATAQDFIEQKKLLEDVLAPVLLKLNAQPE 216
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Zebrafish
Match: ankrd45 (ankyrin repeat domain 45 [Source:ZFIN;Acc:ZDB-GENE-050522-311])

HSP 1 Score: 81.6481 bits (200), Expect = 1.457e-18
Identity = 41/140 (29.29%), Postives = 75/140 (53.57%), Query Frame = 3 
Query:  282 INQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMNDNPD 701
            ++++GYS +H + +W +LD LK + +L  D      R E  + ++ RY   D   YL W  A+ +L  ++ + R +V   EK QG+L K+DK +C + C  +  W+ +   A  +DF  + +  + +L  +  K+N  P+
Sbjct:   33 VHETGYSPLHCSAMWGQLDTLKTLVELNADFQAINFRGEKAVDVARRYDKLDCAEYLAWAEAKQNLQAFIQEVRAIVADQEKVQGKLNKEDKNICINTCSAKSDWINNTRTATAQDFIEQKKLLEDVLAPVLLKLNAQPE 172
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Xenopus
Match: drd1 (dopamine receptor D1 [Source:Xenbase;Acc:XB-GENE-6035431])

HSP 1 Score: 80.4925 bits (197), Expect = 1.128e-17
Identity = 46/184 (25.00%), Postives = 87/184 (47.28%), Query Frame = 3 
Query:  156 EYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMNDNPDEA 707
            E + +LL  K+   +  +  + IL R ++ K LI      N +   GYS +H A  W +LD LK + ++G D+       E    +++RY   +  ++L W  A+  L  Y++     +  +EK QG+L K+ K    + C  +  WL+   +    DF  +  + ++++  I+ K+  + D  
Sbjct:   36 EKVTDLLLKKDFMGRNVLFPACILGRCEVVKELIKYGASVNSLTSRGYSPLHCAAAWGQLDMLKTLVEMGADIKACNFCNEKAYEIAIRYNKTECADFLAWAEAKLELKIYISFVHQSITDLEKLQGKLNKEYKNQTMAACKSKNEWLEHTKNPTTHDFVEQKLQLEAIMQTIFSKLKTSSDSG 219
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Xenopus
Match: drd1 (dopamine receptor D1 [Source:Xenbase;Acc:XB-GENE-6035431])

HSP 1 Score: 68.9366 bits (167), Expect = 2.976e-13
Identity = 38/134 (28.36%), Postives = 66/134 (49.25%), Query Frame = 3 
Query:  156 EYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDK 557
            E + +LL  K+   +  +  + IL R ++ K LI      N +   GYS +H A  W +LD LK + ++G D+       E    +++RY   +  ++L W  A+  L  Y++     +  +EK QG+L K+ K
Sbjct:   36 EKVTDLLLKKDFMGRNVLFPACILGRCEVVKELIKYGASVNSLTSRGYSPLHCAAAWGQLDMLKTLVEMGADIKACNFCNEKAYEIAIRYNKTECADFLAWAEAKLELKIYISFVHQSITDLEKLQGKLNKEYK 169
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Mouse
Match: Ankrd45 (ankyrin repeat domain 45 [Source:MGI Symbol;Acc:MGI:1921094])

HSP 1 Score: 79.337 bits (194), Expect = 3.105e-17
Identity = 46/179 (25.70%), Postives = 88/179 (49.16%), Query Frame = 3 
Query:  153 REYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMN 689
             E+  +LL  +++  +  +  + +  + D+ K L       NE    GY+ +H A  W RL+ LK + +L  D+     R E    ++ RY   +  N+L W +AR  L   +  +  ++   EK  G+L K+DK    + C  +  WL+S+P+A+I +   + ++ + ++  I  KM+
Sbjct:   62 HEHAVQLLLEEDIVGRNLLYAACMAGKSDVIKALAKYGVNLNEATARGYTLLHCAAAWGRLETLKALVELDVDIEALNFRGEKARDVAARYSQVECVNFLDWADARLILKKIITKSSLIITDPEKGPGKLFKEDKSTILNACRLKNEWLESHPEASISEIFEQKQQLEDIVSPILAKMS 240
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Mouse
Match: Ankrd45 (ankyrin repeat domain 45 [Source:MGI Symbol;Acc:MGI:1921094])

HSP 1 Score: 78.9518 bits (193), Expect = 7.045e-17
Identity = 46/178 (25.84%), Postives = 88/178 (49.44%), Query Frame = 3 
Query:  156 EYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMN 689
            E+  +LL  +++  +  +  + +  + D+ K L       NE    GY+ +H A  W RL+ LK + +L  D+     R E    ++ RY   +  N+L W +AR  L   +  +  ++   EK  G+L K+DK    + C  +  WL+S+P+A+I +   + ++ + ++  I  KM+
Sbjct:   97 EHAVQLLLEEDIVGRNLLYAACMAGKSDVIKALAKYGVNLNEATARGYTLLHCAAAWGRLETLKALVELDVDIEALNFRGEKARDVAARYSQVECVNFLDWADARLILKKIITKSSLIITDPEKGPGKLFKEDKSTILNACRLKNEWLESHPEASISEIFEQKQQLEDIVSPILAKMS 274
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Mouse
Match: Ankrd45 (ankyrin repeat domain 45 [Source:MGI Symbol;Acc:MGI:1921094])

HSP 1 Score: 64.6994 bits (156), Expect = 3.677e-12
Identity = 37/142 (26.06%), Postives = 66/142 (46.48%), Query Frame = 3 
Query:  153 REYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVC 578
             E+  +LL  +++  +  +  + +  + D+ K L       NE    GY+ +H A  W RL+ LK + +L  D+     R E    ++ RY   +  N+L W +AR  L   +  +  ++   EK  G+L K+DK    + C
Sbjct:   62 HEHAVQLLLEEDIVGRNLLYAACMAGKSDVIKALAKYGVNLNEATARGYTLLHCAAAWGRLETLKALVELDVDIEALNFRGEKARDVAARYSQVECVNFLDWADARLILKKIITKSSLIITDPEKGPGKLFKEDKSTILNAC 203
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. UniProt/SwissProt
Match: sp|Q810N6|ANR45_MOUSE (Ankyrin repeat domain-containing protein 45 OS=Mus musculus OX=10090 GN=Ankrd45 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 2.172e-16
Identity = 46/179 (25.70%), Postives = 88/179 (49.16%), Query Frame = 3 
Query:  153 REYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMN 689
             E+  +LL  +++  +  +  + +  + D+ K L       NE    GY+ +H A  W RL+ LK + +L  D+     R E    ++ RY   +  N+L W +AR  L   +  +  ++   EK  G+L K+DK    + C  +  WL+S+P+A+I +   + ++ + ++  I  KM+
Sbjct:   62 HEHAVQLLLEEDIVGRNLLYAACMAGKSDVIKALAKYGVNLNEATARGYTLLHCAAAWGRLETLKALVELDVDIEALNFRGEKARDVAARYSQVECVNFLDWADARLILKKIITKSSLIITDPEKGPGKLFKEDKSTILNACRLKNEWLESHPEASISEIFEQKQQLEDIVSPILAKMS 240
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. UniProt/SwissProt
Match: sp|Q5TZF3|ANR45_HUMAN (Ankyrin repeat domain-containing protein 45 OS=Homo sapiens OX=9606 GN=ANKRD45 PE=1 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 5.340e-15
Identity = 48/177 (27.12%), Postives = 85/177 (48.02%), Query Frame = 3 
Query:  162 LDELLSSKNLFNQTPIDYSIILA-RFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMN 689
            L+E +  +NL       Y+  +A + D+ + L       NE    GY+ +H A  W RL+ LK + +L  D+     R E    ++ RY   +   +L W +AR +L  Y+      V   EK  G+L K+DK    S C  +  WL+++ +A+I +   + ++ + ++  I+ KM 
Sbjct:   71 LEEDIVGRNLL------YAACMAGQSDVIRALAKYGVNLNEKTTRGYTLLHCAAAWGRLETLKALVELDVDIEALNFREERARDVAARYSQTECVEFLDWADARLTLKKYIAKVSLAVTDTEKGSGKLLKEDKNTILSACRAKNEWLETHTEASINELFEQRQQLEDIVTPIFTKMT 241
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. TrEMBL
Match: W4Y610 (ANK_REP_REGION domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 5.073e-34
Identity = 63/173 (36.42%), Postives = 105/173 (60.69%), Query Frame = 3 
Query:  186 NLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMNDNPDE 704
            ++  +TP+D + IL R +  K L++     N   + GY++IH+A  W R+D LK + + G  + +KTK  E     +LRY ++D + YL W+ AR  L+  + +T+  +   +K QGRL KD+K    +VC E++ WL SNP+A+IEDFR + E+ ++ L+ I  K+++ P E
Sbjct:   97 SMSARTPLDMACILGRNEALKALLAGGADANNSTKRGYTAIHHAAAWGRMDCLKTLVKYGASLTIKTKHGERARDTALRYKHEDCSFYLDWSEARRGLVSILQETKETIEDPQKLQGRLAKDEKMTGLNVCGEKQEWLDSNPNASIEDFRKQEEDLRTSLEAILIKLSEPPPE 269
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. TrEMBL
Match: A0A267G7K7 (ANK_REP_REGION domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000887g6 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 2.246e-27
Identity = 62/199 (31.16%), Postives = 110/199 (55.28%), Query Frame = 3 
Query:   66 LSSSELFELIENESLTEITNRINNDSLISREYLDELLSSKNLFNQTPIDYSIILARFDIFKVLI-SKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDF---RIKLEE 650
            L+   LF+LI+  S   + + ++N      E + +L+S ++   ++P+D ++ + R +I K L+ ++    N  N  GY+++H A  WR  + LK IY  G D+   ++  E P+ ++ RYG  +  ++L+W  ARD+    +   R  +   EK QGRL KD++K  +  C ERE WL+   DA  ++F   R++LE+
Sbjct:    8 LTQHALFDLIQRGSYRAMIDALDNPIAHPPEVIAQLMSGRSELGKSPLDTAVFMCRQEIVKDLVLNRGADVNATNAEGYTAMHLAAAWRNAEALKIIYAQGGDIQRCSRFGERPIEVAQRYGNSECVDFLQWAEARDAFAAAIRQMRETLADTEKLQGRLNKDERKQADLACTEREQWLQGAQDATTQNFIAMRLELED 206
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. TrEMBL
Match: A0A210QBL4 (Ankyrin repeat domain-containing protein 45 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT04317 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.826e-25
Identity = 58/192 (30.21%), Postives = 100/192 (52.08%), Query Frame = 3 
Query:  117 ITNRINNDSLISREYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMND 692
            +T    +D    +E + EL++ ++   +TP+D +  L R DI K LI++    N +   GY ++H+A  W R+  LK + +    +  +    E P   +LRY   +  +YL W  A+ +L D +   +  +   EK QGRL KDDK L  + C E+  W+ S  DA  +DF I+ +    +L  I++K+++
Sbjct:   43 LTKSFESDEDPYKETVAELINQRDEDGKTPLDIAATLGRVDIAKELIARGADVNSVTTKGYCALHHASAWGRIGVLKVLIEAQAGLQQRNTNNERPRETALRYHQTECVDYLDWAEAKVALQDAIRTAQETLADPEKVQGRLNKDDKNLTLNTCKEKTEWVDSTTDATTQDFLIQKQAFDEILAPIYQKLSE 234
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. TrEMBL
Match: A0A1S3JTV0 (ankyrin repeat domain-containing protein 45 OS=Lingula unguis OX=7574 GN=LOC106176078 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 2.136e-25
Identity = 59/175 (33.71%), Postives = 94/175 (53.71%), Query Frame = 3 
Query:  168 ELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMND 692
            E L+ +N   ++P+D S  L R D+ K LI++  + N  +  GY  +H A  W R+D LK +   G D+  KT   E    ++LRY + +  +YL W  A+  L D +   +  +   EK QGRLTKDD+ +C + C E+  W+    DA  +DF +K +E   +   IW K+++
Sbjct:   52 EHLNQRNDEGKSPLDLSSTLGRLDMTKELITRGGEVNSSSCQGYIPLHRAAAWGRIDVLKLLVDNGADLQQKTVHGERAREVALRYNHAECVDYLDWAEAKVFLQDTIRAMQETLADPEKIQGRLTKDDRNICMNACKEKSDWVDLTVDATTQDFILKKQELDEIFAPIWVKLSE 226
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. TrEMBL
Match: A0A267F371 (ANK_REP_REGION domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000887g1 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 5.234e-25
Identity = 59/194 (30.41%), Postives = 105/194 (54.12%), Query Frame = 3 
Query:   27 EMSLHEGIANENPLSSSELFELIENESLTEITNRINNDSLISREYLDELLSSKNLFNQTPIDYSIILARFDIFKVLI-SKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKS 605
            +M + EG   +  L+   LF+LI+  S   + + ++N      E + +L+S ++   ++P+D ++ + R +I K L+ ++    N  N  GY+++H A  WR  + LK IY  G D+   ++  E P+ ++ RYG  +  ++L+W  ARD+    +   R  +   EK QGRL KD++K  +  C ERE WL+ 
Sbjct:    1 DMEIEEGEPVQ--LTQHALFDLIQRGSYRAMIDALDNPIAHPPEVIAQLMSGRSELGKSPLDTAVFMCRQEIVKDLVLNRGADVNATNAEGYTAMHLAAAWRNAEALKIIYAQGGDIQRCSRFGERPIEVAQRYGNSECVDFLQWAEARDAFAAAIRQMRETLADTEKLQGRLNKDERKQADLACTEREQWLQG 192
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Nematostella
Match: EDO47171 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RMV9])

HSP 1 Score: 78.5666 bits (192), Expect = 1.098e-17
Identity = 46/174 (26.44%), Postives = 84/174 (48.28%), Query Frame = 3 
Query:  174 LSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPD-AAIEDFRIKLEECQSMLDCIWKKMND 692
            L+ +N   ++ ID + +L R ++ + L+ +  + N   + GY+ +H A  W ++  LK +   G D+ ++    E     + RY   D   YL    A+  L   +  T+  +   +K  GR TKDD+      C E+  WL++N D A++E+   + E+   +L  I  K+ D
Sbjct:   36 LNKRNPDGKSAIDLAAMLGRNEVVRELLERGAEVNSKTKKGYTCLHIAACWGQVGCLKALVASGADLQIRNAHGERAREAATRYNKVDCIEYLDKAEAQFELKALITSTKETIADPDKHMGRFTKDDRVSGNRYCDEKSEWLENNADTASLEEIVKQKEDLAGVLQPILSKLED 209
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Medaka
Match: hspa5 (heat shock protein family A (Hsp70) member 5 [Source:NCBI gene;Acc:101157378])

HSP 1 Score: 47.3654 bits (111), Expect = 8.479e-6
Identity = 25/75 (33.33%), Postives = 42/75 (56.00%), Query Frame = 3 
Query:  462 NARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKM 686
            +AR+ L  Y    +N +   EK  G+L+ DDK+  E    E+  W++S+ DA  EDF+ K +E + ++  I  K+
Sbjct:  558 DARNELESYAYSLKNQIGDKEKLGGKLSDDDKETIEKAVEEKIEWMESHQDAETEDFQAKKKELEEVVQPIITKL 632
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Medaka
Match: hspa5 (heat shock protein family A (Hsp70) member 5 [Source:NCBI gene;Acc:101157378])

HSP 1 Score: 47.3654 bits (111), Expect = 8.479e-6
Identity = 25/75 (33.33%), Postives = 42/75 (56.00%), Query Frame = 3 
Query:  462 NARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKM 686
            +AR+ L  Y    +N +   EK  G+L+ DDK+  E    E+  W++S+ DA  EDF+ K +E + ++  I  K+
Sbjct:  558 DARNELESYAYSLKNQIGDKEKLGGKLSDDDKETIEKAVEEKIEWMESHQDAETEDFQAKKKELEEVVQPIITKL 632
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BLAST of Sodium/hydrogen exchanger 11 vs. Planmine SMEST
Match: SMESG000052479.1 (SMESG000052479.1)

HSP 1 Score: 454.521 bits (1168), Expect = 1.780e-164
Identity = 223/226 (98.67%), Postives = 224/226 (99.12%), Query Frame = 3 
Query:   30 MSLHEGIANENPLSSSELFELIENESLTEITNRINNDSLISREYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIEDFRIKLEECQSMLDCIWKKMNDNPDEA 707
            MSLHEGIANENPLSSSELFELIENESLTEITNRINNDSLISREYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPL MSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDA IEDFRIKLEECQ+MLDCIWKKMNDNPDEA
Sbjct:    1 MSLHEGIANENPLSSSELFELIENESLTEITNRINNDSLISREYLDELLSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLKMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDATIEDFRIKLEECQNMLDCIWKKMNDNPDEA 226
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BLAST of Sodium/hydrogen exchanger 11 vs. Planmine SMEST
Match: SMESG000069194.1 (SMESG000069194.1)

HSP 1 Score: 50.8322 bits (120), Expect = 3.975e-7
Identity = 43/167 (25.75%), Postives = 77/167 (46.11%), Query Frame = 3 
Query:  186 NLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARD--SLIDYMNDTRNLVNSIEKSQGRLTKD----DKKLCE-----SVCIERELWLKSNPDAAIEDFRIKLEEC 653
            N+ + TP+  +      +  K+LI+     N +N+ G + +HYA  W  +   KH+ + G  + ++ K L  PL  +  +  D+  +  K + ++D   LI Y + ++    +  K Q  LTK+    D KL +     SV    E+W  S  D  I    +K+ +C
Sbjct:   64 NMGDDTPLHLAASQGHLEAVKLLITLGGNVNAVNEHGNTPLHYACFWNHVHVAKHLIRNGALLMIQNKYLHSPLDKARMHMLDEFIDLAK-SGSKDLMRLIPYKDSSQYGTKARSKDQ-NLTKNEGSLDPKLLKLSGRLSVSHGGEVWKGSYRDVDIVVKLLKMRQC 228
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Planmine SMEST
Match: SMESG000024844.1 (SMESG000024844.1)

HSP 1 Score: 49.2914 bits (116), Expect = 8.237e-7
Identity = 35/139 (25.18%), Postives = 68/139 (48.92%), Query Frame = 3 
Query:  177 SSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSLIDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIEREL 593
              KN F   P+  +     ++I K+LI+        ++ G   IH A ++  +D LK +  +GCD+  K+K  E PL + + Y + +  + +K+  A + L       +N +  I +    L + ++++C S+C  +EL
Sbjct:   75 CDKNGF--PPLHLAYKNGHYEIVKILINNKCGIESFDKFGMRPIHLACLYSHIDILKLLLVIGCDLDSKSKMEESPLDICIHYRHQECIDIMKF--AIELLPKIRELHKNEIKQIFEKFEILNRKNRQMC-SICCRKEL 208
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Planmine SMEST
Match: SMESG000063648.1 (SMESG000063648.1)

HSP 1 Score: 48.9062 bits (115), Expect = 2.051e-6
Identity = 25/94 (26.60%), Postives = 50/94 (53.19%), Query Frame = 3 
Query:  174 LSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLK 455
            +S++N F  TP+   +      + ++LIS  T  N+ NQ+G +++H A   +R    K + + G  + ++  + E PL ++L+  + +I   LK
Sbjct:  213 ISAQNRFGDTPLHTGVRYGHAGVSRILISVSTDINQRNQNGDTALHIAAALKRRKITKLLVESGASIDIRNIQNETPLDVALKKSHSEIIEILK 306
[Close]
BLAST of Sodium/hydrogen exchanger 11 vs. Planmine SMEST
Match: SMESG000063648.1 (SMESG000063648.1)

HSP 1 Score: 48.9062 bits (115), Expect = 2.118e-6
Identity = 25/94 (26.60%), Postives = 50/94 (53.19%), Query Frame = 3 
Query:  174 LSSKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWRRLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLK 455
            +S++N F  TP+   +      + ++LIS  T  N+ NQ+G +++H A   +R    K + + G  + ++  + E PL ++L+  + +I   LK
Sbjct:  180 ISAQNRFGDTPLHTGVRYGHAGVSRILISVSTDINQRNQNGDTALHIAAALKRRKITKLLVESGASIDIRNIQNETPLDVALKKSHSEIIEILK 273
The following BLAST results are available for this feature:
BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
AL139142.21.112e-1527.12novel protein[more]
ANKRD451.112e-1527.12ankyrin repeat domain 45 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ankrd459.825e-2125.77ankyrin repeat domain 45 [Source:ZFIN;Acc:ZDB-GENE... [more]
ankrd451.457e-1829.29ankyrin repeat domain 45 [Source:ZFIN;Acc:ZDB-GENE... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
drd11.128e-1725.00dopamine receptor D1 [Source:Xenbase;Acc:XB-GENE-6... [more]
drd12.976e-1328.36dopamine receptor D1 [Source:Xenbase;Acc:XB-GENE-6... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Ankrd453.105e-1725.70ankyrin repeat domain 45 [Source:MGI Symbol;Acc:MG... [more]
Ankrd457.045e-1725.84ankyrin repeat domain 45 [Source:MGI Symbol;Acc:MG... [more]
Ankrd453.677e-1226.06ankyrin repeat domain 45 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q810N6|ANR45_MOUSE2.172e-1625.70Ankyrin repeat domain-containing protein 45 OS=Mus... [more]
sp|Q5TZF3|ANR45_HUMAN5.340e-1527.12Ankyrin repeat domain-containing protein 45 OS=Hom... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
W4Y6105.073e-3436.42ANK_REP_REGION domain-containing protein OS=Strong... [more]
A0A267G7K72.246e-2731.16ANK_REP_REGION domain-containing protein (Fragment... [more]
A0A210QBL41.826e-2530.21Ankyrin repeat domain-containing protein 45 OS=Miz... [more]
A0A1S3JTV02.136e-2533.71ankyrin repeat domain-containing protein 45 OS=Lin... [more]
A0A267F3715.234e-2530.41ANK_REP_REGION domain-containing protein (Fragment... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO471711.098e-1726.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hspa58.479e-633.33heat shock protein family A (Hsp70) member 5 [Sour... [more]
hspa58.479e-633.33heat shock protein family A (Hsp70) member 5 [Sour... [more]
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BLAST of Sodium/hydrogen exchanger 11 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052479.11.780e-16498.67SMESG000052479.1[more]
SMESG000069194.13.975e-725.75SMESG000069194.1[more]
SMESG000024844.18.237e-725.18SMESG000024844.1[more]
SMESG000063648.12.051e-626.60SMESG000063648.1[more]
SMESG000063648.12.118e-626.60SMESG000063648.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004224 ID=SMED30004224|Name=Sodium/hydrogen exchanger 11|organism=Schmidtea mediterranea sexual|type=transcript|length=777bp
TTTCTATAGAAACTTAAATTAATTTTGAAATGTCATTGCATGAAGGGATC
GCAAATGAAAATCCTTTATCTAGTAGCGAATTATTTGAGTTAATTGAAAA
TGAATCATTAACAGAAATAACAAACCGGATAAATAATGATTCGCTCATTT
CTCGCGAGTATCTTGACGAGTTATTAAGTTCAAAAAATTTATTCAATCAA
ACTCCAATTGATTATTCAATCATTTTAGCAAGATTTGATATTTTTAAAGT
ATTAATATCAAAATTAACAAAATTCAATGAAATAAATCAATCTGGGTACT
CGTCCATTCATTATGCTGTTGTTTGGAGGCGACTTGATGAACTTAAACAT
ATTTACCAATTAGGGTGTGATATGCACCTTAAAACAAAAAGACTCGAAGA
TCCTTTAATGATGTCTCTGAGATATGGCTATGATGACATAACAAACTATT
TGAAATGGACAAATGCTCGAGATTCTCTAATAGATTACATGAATGACACA
AGAAATCTCGTCAATTCCATTGAGAAATCTCAAGGCCGTCTTACAAAGGA
TGATAAAAAGTTGTGTGAGTCTGTGTGTATCGAGCGCGAGCTGTGGCTGA
AATCCAATCCCGATGCCGCAATCGAGGATTTCCGAATTAAGCTGGAGGAG
TGTCAAAGTATGCTGGATTGTATATGGAAAAAAATGAATGACAATCCCGA
TGAGGCTTGAGATAGTTTTGTTTTAATTGGCTTGAAAATTTTAATAAATA
ATCAATCGGTTTTTTTTTGAAAAAAAA
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protein sequence of SMED30004224-orf-1

>SMED30004224-orf-1 ID=SMED30004224-orf-1|Name=SMED30004224-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=227bp
MSLHEGIANENPLSSSELFELIENESLTEITNRINNDSLISREYLDELLS
SKNLFNQTPIDYSIILARFDIFKVLISKLTKFNEINQSGYSSIHYAVVWR
RLDELKHIYQLGCDMHLKTKRLEDPLMMSLRYGYDDITNYLKWTNARDSL
IDYMNDTRNLVNSIEKSQGRLTKDDKKLCESVCIERELWLKSNPDAAIED
FRIKLEECQSMLDCIWKKMNDNPDEA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006812cation transport
GO:1902600hydrogen ion transmembrane transport
GO:0008150biological_process
Vocabulary: molecular function
TermDefinition
GO:0015299solute:proton antiporter activity
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005575cellular_component
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR029048HSP70_C_sf
IPR020683Ankyrin_rpt-contain_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029048Heat shock protein 70kD, C-terminal domain superfamilyGENE3DG3DSA:1.20.1270.10coord: 139..221
e-value: 6.4E-10
score: 41.1
IPR029048Heat shock protein 70kD, C-terminal domain superfamilySUPERFAMILYSSF100934Heat shock protein 70kD (HSP70), C-terminal subdomaincoord: 146..223
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 14..138
e-value: 2.3E-9
score: 39.1
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 49..160
NoneNo IPR availablePANTHERPTHR24180:SF14ANKYRIN REPEAT DOMAIN 45coord: 38..223
NoneNo IPR availablePANTHERPTHR24180FAMILY NOT NAMEDcoord: 38..223
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 55..153
score: 14.026