SMED30004176

Overview
NameSMED30004176
Smed IDSMED30004176
Length (bp)1307
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30004176 (SMED30004176) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30004176 (SMED30004176) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30004176 (SMED30004176) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30004176 (SMED30004176) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30004176

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 31

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30004176h1SMcG0006787 SmedASXL_061618SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig38791newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig38791uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig39149newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig39149uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006788 Contig39149newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006788 Contig39149uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig31297uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig31297newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig32555newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig32555uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30004176h1SMcG0006787 dd_Smed_v4_1693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004176h1SMcG0006787 dd_Smed_v4_1693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004176h1SMcG0006787 dd_Smed_v4_1693_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0006787 Contig563GPL15192PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30004176h1SMcG0006787 dd_Smed_v4_3684_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004176h1SMcG0006787 dd_Smed_v4_3684_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30004176h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004176h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004176h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004176h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004176h1SMcG0006787 dd_Smed_v4_2265_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004176h1SMcG0019490 Contig563GPL15192PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30004176h1SMcG0006787 dd_Smed_v6_1693_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
pharynx musculatureSMED30004176h1SMcG0006787 dd_Smed_v6_2265_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30004176h1SMcG0006787 dd_Smed_v6_2265_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30004176h1SMcG0006787 dd_Smed_v6_1693_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30004176h1SMcG0006787 dd_Smed_v6_1693_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30004176h1SMcG0006787 Contig15693newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30004176h1SMcG0006787 Contig15693uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
testis primordiumSMED30004176h1SMcG0006787 Contig5941_SE3GPL10652PMID:20844018
Wang et al., 2010
whole organism juvenile stagecDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30004176 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005101:9346..17108 -6473
v31.005101:16155..17887 -1324
Homology
BLAST of SMED30004176 vs. TrEMBL
Match: A0A267GCL4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005866g2 PE=4 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 1.237e-34
Identity = 92/151 (60.93%), Postives = 106/151 (70.20%), Query Frame = 2
Query:   74 SKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKS--EDSDGDGIPDNIDK 520
            +K  +  +  P+T DSD DGIPD+LE DSDGDGIPDY EDEDGDGIPDYLE DSDGDGIPDYLEDE+GDGIPDYLE D DGDG PDY +D DGDGIPD+   K   S++ +K  P+A        KK   Q  K    DSD DGI D +D+
Sbjct: 1036 AKASKKSAPAPATPDSDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLEGDSDGDGIPDYLEDEDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDH---KDKDSDYKQK--PAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDR 1181          

HSP 2 Score: 110.538 bits (275), Expect = 4.589e-22
Identity = 72/123 (58.54%), Postives = 82/123 (66.67%), Query Frame = 2
Query:  191 DEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSN--HRKSACKSK 553
            D DGDGIPD+LE DSDGDGIPDYLEDE+GDGIPDYLE DSDGDGIPDY EDEDGDGIPDY ++                      L  D  Q D  +DSDGDGIPD+ DK +   +K A ++K
Sbjct: 1050 DSDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLEGDSDGDGIPDYLEDEDGDGIPDYLELD---------------------LDGDG-QPDYLKDSDGDGIPDHKDKDSDYKQKPAARAK 1150          

HSP 3 Score: 76.6406 bits (187), Expect = 5.994e-11
Identity = 65/160 (40.62%), Postives = 77/160 (48.12%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKE-DEDGDGIPDYLENDSDGDGIPDY--------------------------------LEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIK 382
            GIPDYL+                               DSD DGIPDYLE D DGDGIPDY E D DGDG PDYL+ DSDGDGIPD+                                L D + DGI DYL++D+DG+G+PD  ED + DG+ D  + K
Sbjct: 1080 GIPDYLE------------------------------GDSDGDGIPDYLE-DEDGDGIPDYLELDLDGDGQPDYLK-DSDGDGIPDHKDKDSDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSK 1207          

HSP 4 Score: 75.8702 bits (185), Expect = 9.656e-11
Identity = 58/139 (41.73%), Postives = 77/139 (55.40%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIP--------------------------DYLEDENGDGIPDYLEK-DSDGDGI 325
            GIPD+ DKD   K   KP          + P++ PS K   DSD DGI DYL++D+DG+G+PD  ED + DG+ D L++ DSDGDGIP                          D  EDE+ DG+PDYL++ D+DGDG+
Sbjct: 1130 GIPDHKDKDSDYKQ--KPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGVADSKDKDDDGDKITDKTEDEDRDGVPDYLQQEDTDGDGV 1266          
BLAST of SMED30004176 vs. TrEMBL
Match: A0A267E7Z3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005866g1 PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 9.062e-33
Identity = 90/151 (59.60%), Postives = 105/151 (69.54%), Query Frame = 2
Query:   74 SKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKS--EDSDGDGIPDNIDK 520
            +K  +  +  P+T DSD DGIPD+LE DSDGDGIPDY EDEDGDGIPDYLE DSDGDGI DYLED++GDGIPDYLE D DGDG PDY +D DGDGIPD+   K   S++ +K  P+A        KK   Q  K    DSD DGI D +D+
Sbjct: 1035 AKASKKSAPAPATPDSDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLEGDSDGDGILDYLEDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDH---KDKDSDYKQK--PAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDR 1180          

HSP 2 Score: 77.411 bits (189), Expect = 2.827e-11
Identity = 58/146 (39.73%), Postives = 75/146 (51.37%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPD-------------------YLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIK 382
            GIPDYL+ D  G  ++  +                  +D D DGIPDYLE D DGDG PDY +D DGDGIPD+ + DSD    P                     L D + DGI DYL++D+DG+G+PD  ED + DG+ D  + K
Sbjct: 1079 GIPDYLEGDSDGDGILDYL------------------EDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDHKDKDSDYKQKPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSK 1206          

HSP 3 Score: 74.7146 bits (182), Expect = 1.955e-10
Identity = 58/139 (41.73%), Postives = 77/139 (55.40%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIP--------------------------DYLEDENGDGIPDYLEK-DSDGDGI 325
            GIPD+ DKD   K   KP          + P++ PS K   DSD DGI DYL++D+DG+G+PD  ED + DG+ D L++ DSDGDGIP                          D  EDE+ DG+PDYL++ D+DGDG+
Sbjct: 1129 GIPDHKDKDSDYKQ--KPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGVADSKDKDDDGDKITDKTEDEDRDGVPDYLQQEDTDGDGV 1265          
BLAST of SMED30004176 vs. TrEMBL
Match: A0A1I8GZZ2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.057e-32
Identity = 101/186 (54.30%), Postives = 118/186 (63.44%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENP-STKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHR---KSACK 547
            GIPD+L+ D  G  +   +  E       +P   P   +DSD DGIPD+L+KDSDGDGIPD+ EDEDGDGIPD+LE DSDGDGIPDYLED++GDGIP+YLE DSDGDGIPDY EDEDGDGIPDY ++                      L  D  Q D  +DSDGDGIPD   K   R   K+A K
Sbjct:  524 GIPDHLEGDSDGDGIPDYLEDED---GDGIPDGIPDHLEDSDGDGIPDHLDKDSDGDGIPDHLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLEL---------------------DLDGDG-QPDYLKDSDGDGIPDYKQKPAARAKPKNAAK 684          

HSP 2 Score: 110.538 bits (275), Expect = 3.950e-22
Identity = 93/238 (39.08%), Postives = 109/238 (45.80%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLE------------KDSDGDGIPDYQE-------------------------DEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQ------------------------------VDKSEDSDGDGIPDNI 514
            GIPD+LDKD  G  +                      +D D DGIPD+LE DSDGDGIPDY ED+DGDGIP+YLE DSDGDGIPDYLEDE+GDGIPDYLE            KDSDGDGIPDY++                         D D DGI DY     +  +      P A+         D +Q                               DK+ED D DG+PD +
Sbjct:  565 GIPDHLDKDSDGDGIPD------------------HLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADY-----LDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGDKDDDGDKITDKNEDEDRDGVPDYL 779          

HSP 3 Score: 76.2554 bits (186), Expect = 6.496e-11
Identity = 58/138 (42.03%), Postives = 77/138 (55.80%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSL----VKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIPDYL---------------------EDENGDGIPDYLEK-DSDGDGI 325
            G PDYL KD  G  +     KP          + P++ PS K   DSD DGI DYL++D+DG+G+PD  ED + DG+ D L++ DSDGDGIPD                       EDE+ DG+PDYL++ D+DGDG+
Sbjct:  653 GQPDYL-KDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGDKDDDGDKITDKNEDEDRDGVPDYLQQEDTDGDGV 789          
BLAST of SMED30004176 vs. TrEMBL
Match: A0A1I8IA15 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.157e-32
Identity = 101/186 (54.30%), Postives = 118/186 (63.44%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENP-STKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHR---KSACK 547
            GIPD+L+ D  G  +   +  E       +P   P   +DSD DGIPD+L+KDSDGDGIPD+ EDEDGDGIPD+LE DSDGDGIPDYLED++GDGIP+YLE DSDGDGIPDY EDEDGDGIPDY ++                      L  D  Q D  +DSDGDGIPD   K   R   K+A K
Sbjct:  524 GIPDHLEGDSDGDGIPDYLEDED---GDGIPDGIPDHLEDSDGDGIPDHLDKDSDGDGIPDHLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLEL---------------------DLDGDG-QPDYLKDSDGDGIPDYKQKPAARAKPKNAAK 684          

HSP 2 Score: 135.576 bits (340), Expect = 2.315e-30
Identity = 96/197 (48.73%), Postives = 111/197 (56.35%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHS---NFTKK---------ITPSASIGKVTKLKKDK-----------------VQVDKSEDSDGDGIP 505
            GIPD+LDKD  G  +                      +D D DGIPD+LE DSDGDGIPDY ED+DGDGIP+YLE DSDGDGIPDYLEDE+GDGIPDYLE D DGDG PDY +D DGDGIPDY +     +   N  KK         +  S S G    L +D                    + +S+DSDGDGIP
Sbjct:  565 GIPDHLDKDSDGDGIPD------------------HLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIP 743          

HSP 3 Score: 66.2402 bits (160), Expect = 9.295e-8
Identity = 58/136 (42.65%), Postives = 77/136 (56.62%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSL----VKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIP-------------------DYLEDENGDGIPDYLEK-DSDGDGI 325
            G PDYL KD  G  +     KP          + P++ PS K   DSD DGI DYL++D+DG+G+PD  ED + DG+ D L++ DSDGDGIP                   D  EDE+ DG+PDYL++ D+DGDG+
Sbjct:  653 GQPDYL-KDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGVADSKDKDDDDEDEDRDGVPDYLQQEDTDGDGV 787          
BLAST of SMED30004176 vs. TrEMBL
Match: A0A1I8HRM3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.170e-30
Identity = 79/139 (56.83%), Postives = 94/139 (67.63%), Query Frame = 2
Query:  122 DEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHS---NFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNH 529
            D DGIPD+LE DSDGDGIPDY ED+DGDGIP+YLE DSDGDGI DYLED++GDGIPDYLE D DGDG PDY +D DGDGIPDY +     +   N  KK     S   +     D +      D+DG+G+PD  + +N 
Sbjct:    2 DGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGILDYLEDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNE 140          

HSP 2 Score: 99.3673 bits (246), Expect = 8.556e-19
Identity = 63/105 (60.00%), Postives = 71/105 (67.62%), Query Frame = 2
Query:  194 EDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPD 508
            EDGDGIPD+LE DSDGDGIPDYLED++GDGIP+YLE DSDGDGI DY ED+DGDGIPDY ++                      L  D  Q D  +DSDGDGIPD
Sbjct:    1 EDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGILDYLEDKDGDGIPDYLEL---------------------DLDGDG-QPDYLKDSDGDGIPD 83          

HSP 3 Score: 95.9005 bits (237), Expect = 1.395e-17
Identity = 62/110 (56.36%), Postives = 71/110 (64.55%), Query Frame = 2
Query:  113 KDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEK------------------------DSDGDGIPDYQE-DEDGDGIPD 367
            +D D DGIP+YLE DSDGDGI DY ED+DGDGIPDYLE D DGDG PDYL+D +GDGIPDY +K                        DSD DGI DY + D DG+G+PD
Sbjct:   24 EDDDGDGIPNYLEGDSDGDGILDYLEDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPD 133          
BLAST of SMED30004176 vs. Ensembl Nematostella
Match: EDO35541 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLA5])

HSP 1 Score: 52.373 bits (124), Expect = 1.157e-7
Identity = 37/99 (37.37%), Postives = 51/99 (51.52%), Query Frame = 2
Query:   89 MPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382
            MPS+  +T+    DG+P  L   + GDG+P       GDG+P  L   + GDG+P  L    GDG+P  L   + GDG+P       GDG+P D H  +
Sbjct:  181 MPSDLHTTR---GDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSNLHT-TRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSDLHTTR 273          

HSP 2 Score: 48.9062 bits (115), Expect = 1.559e-6
Identity = 43/120 (35.83%), Postives = 58/120 (48.33%), Query Frame = 2
Query:   59 LHETLSKKHQMPSENPSTKDSDEDGIPDYL------EKDSD-----GDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382
            LH T  +  +MPS+  +T+    DG+P  L      E  SD     GDG+P       GDG+P  L   + GDG+P  L    GDG+P  L   + GDG+P       GDG+P D H  +
Sbjct:  137 LHTT--RGDEMPSDLHTTR---GDGMPSNLHTTRGDEMPSDFHTIRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSNLHT-TRGDGMPSDLHTTRGDGMPSDLHTTR 249          

HSP 3 Score: 48.9062 bits (115), Expect = 1.631e-6
Identity = 36/99 (36.36%), Postives = 50/99 (50.51%), Query Frame = 2
Query:   89 MPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGI-PDYHKIK 382
            MPS+  +T+    DG+P  L   + GDG+P       GDG+P  L   + GDG+P  L    GDG+P  L   + GDG+P       GDG+  D H  +
Sbjct:  193 MPSDLHTTR---GDGMPSNLHT-TRGDGMPSNLHTTRGDGMPSDLHT-TRGDGMPSDLHTTRGDGMPSNLHT-TRGDGMPSDLHTTRGDGMKSDLHTTR 285          

HSP 4 Score: 46.9802 bits (110), Expect = 6.576e-6
Identity = 39/109 (35.78%), Postives = 55/109 (50.46%), Query Frame = 2
Query:   59 LHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382
            LH T  +  +MPS   +T+    DG+P  L   + GDG+P       GDG+P  L   + GDG+P  L    GD +P  L   + GDG+P       GD +P D+H I+
Sbjct:   77 LHTT--RGDEMPSNLHTTR---GDGMPSDLHT-TRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSDLHTTRGDEMPSDLHT-TRGDGMPSNLHTTRGDEMPSDFHTIR 177          

HSP 5 Score: 46.595 bits (109), Expect = 9.334e-6
Identity = 36/99 (36.36%), Postives = 50/99 (50.51%), Query Frame = 2
Query:   89 MPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382
            MPS+  +T+    DG+P  L   + GDG+P       GD +P  L   + GDG+P  L    GDG+P  L   + GDG+P       GDG+P D H  +
Sbjct:   49 MPSDLHTTR---GDGMPSDLHT-TRGDGMPSDLHTTRGDEMPSNLHT-TRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSDLHTTRGDGMPSDLHTTR 141          
BLAST of SMED30004176 vs. Planmine SMEST
Match: SMESG000054821.1 (SMESG000054821.1)

HSP 1 Score: 445.662 bits (1145), Expect = 6.600e-143
Identity = 228/229 (99.56%), Postives = 228/229 (99.56%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 688
            GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE
Sbjct: 1369 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 1597          

HSP 2 Score: 162.54 bits (410), Expect = 4.485e-43
Identity = 109/200 (54.50%), Postives = 124/200 (62.00%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKES 592
            GIPDYL+KD  G  +  P   E                D D DGIPDYL+ DSDGDGIPDYKEDEDGDGIPDYLE DSDGDGIPDY+EDENGDGIPDYLEKDSDGDGIPDY+EDEDGDGIPDY +           +      G    L+KD       D  ED+DGDGIPD +DK     +  K       + +LK++S
Sbjct: 1034 GIPDYLEKDSDGDGI--PDYKE----------------DEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDS 1215          

HSP 3 Score: 160.999 bits (406), Expect = 1.569e-42
Identity = 107/176 (60.80%), Postives = 121/176 (68.75%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDK 520
            GIPDYLDKD +  ++ K       SK + + +     +DSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGIPDY EDE+GDGIPDYLEKDSDGDGIPDYQEDE+GDGIPDY +              S   G    L KD       +  ED+DGDGIPD +DK
Sbjct: 1184 GIPDYLDKDTESTNIKK-----ESSKNNVLDN---LKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYQEDENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDK 1351          

HSP 4 Score: 148.288 bits (373), Expect = 3.122e-38
Identity = 94/173 (54.34%), Postives = 114/173 (65.90%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDK 520
            GIPDYL+KD  G  +  P   E                D + DGIPDYLEKDSDGDGIPD+KED DGDGIPDYL+ D+DGDGIP+Y ED +GDGIPDYL+KDSDGDGIPDY+EDEDGDGIPDY        +  K +        ++K  +   +   ++DSDGDGIPD ++K
Sbjct: 1269 GIPDYLEKDSDGDGI--PDYQE----------------DENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSLVKPVLHET----LSKKHQMPSENPSTKDSDGDGIPDYLEK 1419          

HSP 5 Score: 54.299 bits (129), Expect = 1.716e-7
Identity = 60/136 (44.12%), Postives = 74/136 (54.41%), Query Frame = 2
Query:    5 IPDYLDK--DYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLE-DENGDGIPDYLEKDSDGDGIP-DYQEDEDGDGIPDYHKIKQIHSNF 400
            IPD+LDK  D   K+  + +L +   + H            D DGIPD  + D D DG  D K+D D DGI +  ++D D DGIPD  + D NGDGIPD + KDSDGDGIP     D+D DGIPD         NF
Sbjct:  259 IPDFLDKNRDVNNKADFEAILADAARQDH------------DCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNF 382          

HSP 6 Score: 50.447 bits (119), Expect = 2.521e-6
Identity = 60/178 (33.71%), Postives = 87/178 (48.88%), Query Frame = 2
Query:  110 TKDSDEDGIPDYLEKDSDGDGIPDYKE-DEDGDGIPDYLEN-----------------DSDGDGIP-------------DYLEDENGDGIPDYLEKDSDGDGIPDY--------QEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK----VQVDKSEDSDGDGIPDNI 514
            T+DSD DG  +++++D D DGIPD ++ D +GDGIPD  E+                 DSD DG+P             D LED +G+ IPD+L+K+ D +   D+        ++D D DGIPD +     +  F  K   S   G + +   D     +   +  DS+GDGIPD I
Sbjct:  170 TQDSDWDGDLNHIDEDDDNDGIPDKEDPDSNGDGIPDCREDSSDNKLKLRFKSRQKRLDSDMDGVPDDSDIDDDQDGIPDILEDSDGNDIPDFLDKNRDVNNKADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCI 347          
BLAST of SMED30004176 vs. Planmine SMEST
Match: SMESG000054821.1 (SMESG000054821.1)

HSP 1 Score: 444.891 bits (1143), Expect = 1.375e-142
Identity = 228/229 (99.56%), Postives = 228/229 (99.56%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 688
            GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE
Sbjct: 1387 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 1615          

HSP 2 Score: 162.925 bits (411), Expect = 3.834e-43
Identity = 109/200 (54.50%), Postives = 124/200 (62.00%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKES 592
            GIPDYL+KD  G  +  P   E                D D DGIPDYL+ DSDGDGIPDYKEDEDGDGIPDYLE DSDGDGIPDY+EDENGDGIPDYLEKDSDGDGIPDY+EDEDGDGIPDY +           +      G    L+KD       D  ED+DGDGIPD +DK     +  K       + +LK++S
Sbjct: 1052 GIPDYLEKDSDGDGI--PDYKE----------------DEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDS 1233          

HSP 3 Score: 161.384 bits (407), Expect = 1.232e-42
Identity = 107/176 (60.80%), Postives = 119/176 (67.61%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDK 520
            GIPDYLDKD +  ++ K       SK + + +     +DSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGIPDY EDE+GDGIPDYLEKDSDGDGIPDYQEDE+GDGIPDY                         L+KD       D  EDSDGDGIPD +DK
Sbjct: 1202 GIPDYLDKDTESTNIKK-----ESSKNNVLDN---LKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYQEDENGDGIPDY-------------------------LEKDSDGDGIPDFKEDSDGDGIPDYLDK 1344          

HSP 4 Score: 148.673 bits (374), Expect = 2.548e-38
Identity = 94/173 (54.34%), Postives = 114/173 (65.90%), Query Frame = 2
Query:    2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDK 520
            GIPDYL+KD  G  +  P   E                D + DGIPDYLEKDSDGDGIPD+KED DGDGIPDYL+ D+DGDGIP+Y ED +GDGIPDYL+KDSDGDGIPDY+EDEDGDGIPDY        +  K +        ++K  +   +   ++DSDGDGIPD ++K
Sbjct: 1287 GIPDYLEKDSDGDGI--PDYQE----------------DENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSLVKPVLHET----LSKKHQMPSENPSTKDSDGDGIPDYLEK 1437          

HSP 5 Score: 54.299 bits (129), Expect = 1.563e-7
Identity = 60/136 (44.12%), Postives = 74/136 (54.41%), Query Frame = 2
Query:    5 IPDYLDK--DYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLE-DENGDGIPDYLEKDSDGDGIP-DYQEDEDGDGIPDYHKIKQIHSNF 400
            IPD+LDK  D   K+  + +L +   + H            D DGIPD  + D D DG  D K+D D DGI +  ++D D DGIPD  + D NGDGIPD + KDSDGDGIP     D+D DGIPD         NF
Sbjct:  277 IPDFLDKNRDVNNKADFEAILADAARQDH------------DCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNF 400          

HSP 6 Score: 50.8322 bits (120), Expect = 2.017e-6
Identity = 60/178 (33.71%), Postives = 87/178 (48.88%), Query Frame = 2
Query:  110 TKDSDEDGIPDYLEKDSDGDGIPDYKE-DEDGDGIPDYLEN-----------------DSDGDGIP-------------DYLEDENGDGIPDYLEKDSDGDGIPDY--------QEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK----VQVDKSEDSDGDGIPDNI 514
            T+DSD DG  +++++D D DGIPD ++ D +GDGIPD  E+                 DSD DG+P             D LED +G+ IPD+L+K+ D +   D+        ++D D DGIPD +     +  F  K   S   G + +   D     +   +  DS+GDGIPD I
Sbjct:  188 TQDSDWDGDLNHIDEDDDNDGIPDKEDPDSNGDGIPDCREDSSDNKLKLRFKSRQKRLDSDMDGVPDDSDIDDDQDGIPDILEDSDGNDIPDFLDKNRDVNNKADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCI 365          
The following BLAST results are available for this feature:
BLAST of SMED30004176 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GCL41.237e-3460.93Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267E7Z39.062e-3359.60Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GZZ21.057e-3254.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IA151.157e-3254.30Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HRM32.170e-3056.83Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30004176 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO355411.157e-737.37Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30004176 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004176 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000054821.16.600e-14399.56SMESG000054821.1[more]
SMESG000054821.11.375e-14299.56SMESG000054821.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004176 ID=SMED30004176|Name=SMED30004176|organism=Schmidtea mediterranea sexual|type=transcript|length=1307bp
TGGAATTCCTGATTATTTAGATAAGGATTATAAAGGAAAATCTTTAGTTA
AACCTGTACTTCATGAGACACTGTCAAAAAAACATCAAATGCCGAGTGAA
AATCCTAGTACAAAAGATTCAGATGAAGATGGAATACCCGATTATTTAGA
AAAAGATTCAGATGGAGATGGAATACCAGATTACAAAGAAGATGAAGATG
GGGATGGAATTCCAGATTATTTAGAAAATGATTCCGATGGTGATGGAATA
CCTGATTACTTAGAAGATGAAAATGGTGACGGAATTCCAGATTATCTTGA
AAAGGATTCTGATGGCGATGGAATACCTGACTATCAAGAAGACGAAGATG
GTGACGGTATACCGGATTATCACAAAATTAAGCAAATCCATTCAAATTTC
ACTAAAAAAATTACCCCTAGTGCTTCAATTGGCAAAGTTACAAAATTAAA
GAAAGATAAAGTTCAGGTCGATAAAAGTGAAGATTCTGATGGTGATGGTA
TTCCAGATAATATTGACAAATCAAATCACAGGAAATCCGCGTGCAAATCA
AAAGAATCGTGCGGTAAAATAATCGATCTAAAAAAGGAATCGATTCAAAA
TGAGATCAACGAAAACAAATTATCAATGCCTCGAATAATAATCCGCCAGG
TTCTCCAATCTCTAGAAAACTTTTTCGGCACTGACGAATAATTGATGGCG
ATTTACAGAATGGAAATCTTTTAACATGACTCTAGAGGCAATTTCCGTAG
ATAAAGTCCATTATTAGTGTTTGATTAATTGCTATTTTTAAATTTTCTGT
TAATAGGTTCTTTAATTTTATGATTAATATTAGATTTCAATCAATTTTAA
GATGCTTACTTCCGACTTTGTTCATTGATATTTATTTTAGCTGAAAAAGT
TTCCCGATTGATTATTCAAATTTTCGTTAGTTTTCGCAACTAATTTTTCG
TGTTGGTTTTCGATTATTAAAACAAAATCCTCCAACAAATTTGTGATTTT
ATTTATCGAAAATATTTTTTACTGAATAATCTTTGATATTTTTTCGTTAA
AATATTCATTTTAAATTCATAGAAGATTTTGCTTTAGTTTTAAAAACAAA
ATTTTTCTGTTACATTGCATCCTTGGAAAGCTTGATCTTTTACATTGGAA
TCAGATTTGATGGGATTCCAGATTGAAAGGTTTTATTTTCGGTGGATTTA
TGGAAATTCGTGATTTTCTCTTTGTATATAATTATTTGCAGCTGTATTTC
AGTTTGATACGGTTAAAATTCACCGTAATTTACGATTAAATATATTTTGT
GCAATCG
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protein sequence of SMED30004176-orf-1

>SMED30004176-orf-1 ID=SMED30004176-orf-1|Name=SMED30004176-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=230bp
GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLE
KDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLE
KDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLK
KDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQN
EINENKLSMPRIIIRQVLQSLENFFGTDE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0000428musculature system
PLANA:0000478pharynx musculature
Vocabulary: INTERPRO
TermDefinition
IPR003367Thrombospondin_3-like_rpt
IPR028974TSP_type-3_rpt
Vocabulary: biological process
TermDefinition
GO:0007155cell adhesion
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003367Thrombospondin, type 3-like repeatPFAMPF02412TSP_3coord: 88..112
e-value: 0.005
score: 16.5
coord: 38..61
e-value: 0.0012
score: 18.5
coord: 63..87
e-value: 0.0026
score: 17.4
IPR028974TSP type-3 repeatGENE3DG3DSA:4.10.1080.10coord: 61..138
e-value: 2.9E-7
score: 32.5
IPR028974TSP type-3 repeatSUPERFAMILYSSF103647TSP type-3 repeatcoord: 33..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..119
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 59..93
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..179
NoneNo IPR availablePANTHERPTHR10199THROMBOSPONDINcoord: 33..123
NoneNo IPR availablePANTHERPTHR10199:SF100THROMBOSPONDIN, ISOFORM Acoord: 33..123