SMED30004176
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004176 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 31Note: Hover over icons to view figure legend
Alignments
SMED30004176 aligns in the following genomic locations:
Homology
BLAST of SMED30004176 vs. TrEMBL
Match: A0A267GCL4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005866g2 PE=4 SV=1) HSP 1 Score: 148.288 bits (373), Expect = 1.237e-34 Identity = 92/151 (60.93%), Postives = 106/151 (70.20%), Query Frame = 2 Query: 74 SKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKS--EDSDGDGIPDNIDK 520 +K + + P+T DSD DGIPD+LE DSDGDGIPDY EDEDGDGIPDYLE DSDGDGIPDYLEDE+GDGIPDYLE D DGDG PDY +D DGDGIPD+ K S++ +K P+A KK Q K DSD DGI D +D+ Sbjct: 1036 AKASKKSAPAPATPDSDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLEGDSDGDGIPDYLEDEDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDH---KDKDSDYKQK--PAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDR 1181 HSP 2 Score: 110.538 bits (275), Expect = 4.589e-22 Identity = 72/123 (58.54%), Postives = 82/123 (66.67%), Query Frame = 2 Query: 191 DEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSN--HRKSACKSK 553 D DGDGIPD+LE DSDGDGIPDYLEDE+GDGIPDYLE DSDGDGIPDY EDEDGDGIPDY ++ L D Q D +DSDGDGIPD+ DK + +K A ++K Sbjct: 1050 DSDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLEGDSDGDGIPDYLEDEDGDGIPDYLELD---------------------LDGDG-QPDYLKDSDGDGIPDHKDKDSDYKQKPAARAK 1150 HSP 3 Score: 76.6406 bits (187), Expect = 5.994e-11 Identity = 65/160 (40.62%), Postives = 77/160 (48.12%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKE-DEDGDGIPDYLENDSDGDGIPDY--------------------------------LEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIK 382 GIPDYL+ DSD DGIPDYLE D DGDGIPDY E D DGDG PDYL+ DSDGDGIPD+ L D + DGI DYL++D+DG+G+PD ED + DG+ D + K Sbjct: 1080 GIPDYLE------------------------------GDSDGDGIPDYLE-DEDGDGIPDYLELDLDGDGQPDYLK-DSDGDGIPDHKDKDSDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSK 1207 HSP 4 Score: 75.8702 bits (185), Expect = 9.656e-11 Identity = 58/139 (41.73%), Postives = 77/139 (55.40%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIP--------------------------DYLEDENGDGIPDYLEK-DSDGDGI 325 GIPD+ DKD K KP + P++ PS K DSD DGI DYL++D+DG+G+PD ED + DG+ D L++ DSDGDGIP D EDE+ DG+PDYL++ D+DGDG+ Sbjct: 1130 GIPDHKDKDSDYKQ--KPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGVADSKDKDDDGDKITDKTEDEDRDGVPDYLQQEDTDGDGV 1266
BLAST of SMED30004176 vs. TrEMBL
Match: A0A267E7Z3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005866g1 PE=4 SV=1) HSP 1 Score: 142.895 bits (359), Expect = 9.062e-33 Identity = 90/151 (59.60%), Postives = 105/151 (69.54%), Query Frame = 2 Query: 74 SKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKS--EDSDGDGIPDNIDK 520 +K + + P+T DSD DGIPD+LE DSDGDGIPDY EDEDGDGIPDYLE DSDGDGI DYLED++GDGIPDYLE D DGDG PDY +D DGDGIPD+ K S++ +K P+A KK Q K DSD DGI D +D+ Sbjct: 1035 AKASKKSAPAPATPDSDGDGIPDHLEGDSDGDGIPDYLEDEDGDGIPDYLEGDSDGDGILDYLEDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDH---KDKDSDYKQK--PAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDR 1180 HSP 2 Score: 77.411 bits (189), Expect = 2.827e-11 Identity = 58/146 (39.73%), Postives = 75/146 (51.37%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPD-------------------YLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIK 382 GIPDYL+ D G ++ + +D D DGIPDYLE D DGDG PDY +D DGDGIPD+ + DSD P L D + DGI DYL++D+DG+G+PD ED + DG+ D + K Sbjct: 1079 GIPDYLEGDSDGDGILDYL------------------EDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDHKDKDSDYKQKPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSK 1206 HSP 3 Score: 74.7146 bits (182), Expect = 1.955e-10 Identity = 58/139 (41.73%), Postives = 77/139 (55.40%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIP--------------------------DYLEDENGDGIPDYLEK-DSDGDGI 325 GIPD+ DKD K KP + P++ PS K DSD DGI DYL++D+DG+G+PD ED + DG+ D L++ DSDGDGIP D EDE+ DG+PDYL++ D+DGDG+ Sbjct: 1129 GIPDHKDKDSDYKQ--KPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGVADSKDKDDDGDKITDKTEDEDRDGVPDYLQQEDTDGDGV 1265
BLAST of SMED30004176 vs. TrEMBL
Match: A0A1I8GZZ2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 142.124 bits (357), Expect = 1.057e-32 Identity = 101/186 (54.30%), Postives = 118/186 (63.44%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENP-STKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHR---KSACK 547 GIPD+L+ D G + + E +P P +DSD DGIPD+L+KDSDGDGIPD+ EDEDGDGIPD+LE DSDGDGIPDYLED++GDGIP+YLE DSDGDGIPDY EDEDGDGIPDY ++ L D Q D +DSDGDGIPD K R K+A K Sbjct: 524 GIPDHLEGDSDGDGIPDYLEDED---GDGIPDGIPDHLEDSDGDGIPDHLDKDSDGDGIPDHLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLEL---------------------DLDGDG-QPDYLKDSDGDGIPDYKQKPAARAKPKNAAK 684 HSP 2 Score: 110.538 bits (275), Expect = 3.950e-22 Identity = 93/238 (39.08%), Postives = 109/238 (45.80%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLE------------KDSDGDGIPDYQE-------------------------DEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQ------------------------------VDKSEDSDGDGIPDNI 514 GIPD+LDKD G + +D D DGIPD+LE DSDGDGIPDY ED+DGDGIP+YLE DSDGDGIPDYLEDE+GDGIPDYLE KDSDGDGIPDY++ D D DGI DY + + P A+ D +Q DK+ED D DG+PD + Sbjct: 565 GIPDHLDKDSDGDGIPD------------------HLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADY-----LDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGDKDDDGDKITDKNEDEDRDGVPDYL 779 HSP 3 Score: 76.2554 bits (186), Expect = 6.496e-11 Identity = 58/138 (42.03%), Postives = 77/138 (55.80%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSL----VKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIPDYL---------------------EDENGDGIPDYLEK-DSDGDGI 325 G PDYL KD G + KP + P++ PS K DSD DGI DYL++D+DG+G+PD ED + DG+ D L++ DSDGDGIPD EDE+ DG+PDYL++ D+DGDG+ Sbjct: 653 GQPDYL-KDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGDKDDDGDKITDKNEDEDRDGVPDYLQQEDTDGDGV 789
BLAST of SMED30004176 vs. TrEMBL
Match: A0A1I8IA15 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 142.124 bits (357), Expect = 1.157e-32 Identity = 101/186 (54.30%), Postives = 118/186 (63.44%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENP-STKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHR---KSACK 547 GIPD+L+ D G + + E +P P +DSD DGIPD+L+KDSDGDGIPD+ EDEDGDGIPD+LE DSDGDGIPDYLED++GDGIP+YLE DSDGDGIPDY EDEDGDGIPDY ++ L D Q D +DSDGDGIPD K R K+A K Sbjct: 524 GIPDHLEGDSDGDGIPDYLEDED---GDGIPDGIPDHLEDSDGDGIPDHLDKDSDGDGIPDHLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLEL---------------------DLDGDG-QPDYLKDSDGDGIPDYKQKPAARAKPKNAAK 684 HSP 2 Score: 135.576 bits (340), Expect = 2.315e-30 Identity = 96/197 (48.73%), Postives = 111/197 (56.35%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHS---NFTKK---------ITPSASIGKVTKLKKDK-----------------VQVDKSEDSDGDGIP 505 GIPD+LDKD G + +D D DGIPD+LE DSDGDGIPDY ED+DGDGIP+YLE DSDGDGIPDYLEDE+GDGIPDYLE D DGDG PDY +D DGDGIPDY + + N KK + S S G L +D + +S+DSDGDGIP Sbjct: 565 GIPDHLDKDSDGDGIPD------------------HLEDEDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGIPDYLEDEDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIP 743 HSP 3 Score: 66.2402 bits (160), Expect = 9.295e-8 Identity = 58/136 (42.65%), Postives = 77/136 (56.62%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSL----VKPVLHETLSKKHQMPSENPSTK---DSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLEN-DSDGDGIP-------------------DYLEDENGDGIPDYLEK-DSDGDGI 325 G PDYL KD G + KP + P++ PS K DSD DGI DYL++D+DG+G+PD ED + DG+ D L++ DSDGDGIP D EDE+ DG+PDYL++ D+DGDG+ Sbjct: 653 GQPDYL-KDSDGDGIPDYKQKPAARAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNEDGMSDLLQSKDSDGDGIPDAQDDDDDNDGVADSKDKDDDDEDEDRDGVPDYLQQEDTDGDGV 787
BLAST of SMED30004176 vs. TrEMBL
Match: A0A1I8HRM3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 132.88 bits (333), Expect = 2.170e-30 Identity = 79/139 (56.83%), Postives = 94/139 (67.63%), Query Frame = 2 Query: 122 DEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHS---NFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNH 529 D DGIPD+LE DSDGDGIPDY ED+DGDGIP+YLE DSDGDGI DYLED++GDGIPDYLE D DGDG PDY +D DGDGIPDY + + N KK S + D + D+DG+G+PD + +N Sbjct: 2 DGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGILDYLEDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPDAAEDNNE 140 HSP 2 Score: 99.3673 bits (246), Expect = 8.556e-19 Identity = 63/105 (60.00%), Postives = 71/105 (67.62%), Query Frame = 2 Query: 194 EDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPD 508 EDGDGIPD+LE DSDGDGIPDYLED++GDGIP+YLE DSDGDGI DY ED+DGDGIPDY ++ L D Q D +DSDGDGIPD Sbjct: 1 EDGDGIPDHLEGDSDGDGIPDYLEDDDGDGIPNYLEGDSDGDGILDYLEDKDGDGIPDYLEL---------------------DLDGDG-QPDYLKDSDGDGIPD 83 HSP 3 Score: 95.9005 bits (237), Expect = 1.395e-17 Identity = 62/110 (56.36%), Postives = 71/110 (64.55%), Query Frame = 2 Query: 113 KDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEK------------------------DSDGDGIPDYQE-DEDGDGIPD 367 +D D DGIP+YLE DSDGDGI DY ED+DGDGIPDYLE D DGDG PDYL+D +GDGIPDY +K DSD DGI DY + D DG+G+PD Sbjct: 24 EDDDGDGIPNYLEGDSDGDGILDYLEDKDGDGIPDYLELDLDGDGQPDYLKDSDGDGIPDYKQKPAAGAKPKNAAKKPAKQPSKKALADSDSDGIADYLDRDADGNGVPD 133
BLAST of SMED30004176 vs. Ensembl Nematostella
Match: EDO35541 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLA5]) HSP 1 Score: 52.373 bits (124), Expect = 1.157e-7 Identity = 37/99 (37.37%), Postives = 51/99 (51.52%), Query Frame = 2 Query: 89 MPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382 MPS+ +T+ DG+P L + GDG+P GDG+P L + GDG+P L GDG+P L + GDG+P GDG+P D H + Sbjct: 181 MPSDLHTTR---GDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSNLHT-TRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSDLHTTR 273 HSP 2 Score: 48.9062 bits (115), Expect = 1.559e-6 Identity = 43/120 (35.83%), Postives = 58/120 (48.33%), Query Frame = 2 Query: 59 LHETLSKKHQMPSENPSTKDSDEDGIPDYL------EKDSD-----GDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382 LH T + +MPS+ +T+ DG+P L E SD GDG+P GDG+P L + GDG+P L GDG+P L + GDG+P GDG+P D H + Sbjct: 137 LHTT--RGDEMPSDLHTTR---GDGMPSNLHTTRGDEMPSDFHTIRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSNLHTTRGDGMPSNLHT-TRGDGMPSDLHTTRGDGMPSDLHTTR 249 HSP 3 Score: 48.9062 bits (115), Expect = 1.631e-6 Identity = 36/99 (36.36%), Postives = 50/99 (50.51%), Query Frame = 2 Query: 89 MPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGI-PDYHKIK 382 MPS+ +T+ DG+P L + GDG+P GDG+P L + GDG+P L GDG+P L + GDG+P GDG+ D H + Sbjct: 193 MPSDLHTTR---GDGMPSNLHT-TRGDGMPSNLHTTRGDGMPSDLHT-TRGDGMPSDLHTTRGDGMPSNLHT-TRGDGMPSDLHTTRGDGMKSDLHTTR 285 HSP 4 Score: 46.9802 bits (110), Expect = 6.576e-6 Identity = 39/109 (35.78%), Postives = 55/109 (50.46%), Query Frame = 2 Query: 59 LHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382 LH T + +MPS +T+ DG+P L + GDG+P GDG+P L + GDG+P L GD +P L + GDG+P GD +P D+H I+ Sbjct: 77 LHTT--RGDEMPSNLHTTR---GDGMPSDLHT-TRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSDLHTTRGDEMPSDLHT-TRGDGMPSNLHTTRGDEMPSDFHTIR 177 HSP 5 Score: 46.595 bits (109), Expect = 9.334e-6 Identity = 36/99 (36.36%), Postives = 50/99 (50.51%), Query Frame = 2 Query: 89 MPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIP-DYHKIK 382 MPS+ +T+ DG+P L + GDG+P GD +P L + GDG+P L GDG+P L + GDG+P GDG+P D H + Sbjct: 49 MPSDLHTTR---GDGMPSDLHT-TRGDGMPSDLHTTRGDEMPSNLHT-TRGDGMPSDLHTTRGDGMPSDLHT-TRGDGMPSDLHTTRGDGMPSDLHTTR 141
BLAST of SMED30004176 vs. Planmine SMEST
Match: SMESG000054821.1 (SMESG000054821.1) HSP 1 Score: 445.662 bits (1145), Expect = 6.600e-143 Identity = 228/229 (99.56%), Postives = 228/229 (99.56%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 688 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE Sbjct: 1369 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 1597 HSP 2 Score: 162.54 bits (410), Expect = 4.485e-43 Identity = 109/200 (54.50%), Postives = 124/200 (62.00%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKES 592 GIPDYL+KD G + P E D D DGIPDYL+ DSDGDGIPDYKEDEDGDGIPDYLE DSDGDGIPDY+EDENGDGIPDYLEKDSDGDGIPDY+EDEDGDGIPDY + + G L+KD D ED+DGDGIPD +DK + K + +LK++S Sbjct: 1034 GIPDYLEKDSDGDGI--PDYKE----------------DEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDS 1215 HSP 3 Score: 160.999 bits (406), Expect = 1.569e-42 Identity = 107/176 (60.80%), Postives = 121/176 (68.75%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDK 520 GIPDYLDKD + ++ K SK + + + +DSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGIPDY EDE+GDGIPDYLEKDSDGDGIPDYQEDE+GDGIPDY + S G L KD + ED+DGDGIPD +DK Sbjct: 1184 GIPDYLDKDTESTNIKK-----ESSKNNVLDN---LKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYQEDENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDK 1351 HSP 4 Score: 148.288 bits (373), Expect = 3.122e-38 Identity = 94/173 (54.34%), Postives = 114/173 (65.90%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDK 520 GIPDYL+KD G + P E D + DGIPDYLEKDSDGDGIPD+KED DGDGIPDYL+ D+DGDGIP+Y ED +GDGIPDYL+KDSDGDGIPDY+EDEDGDGIPDY + K + ++K + + ++DSDGDGIPD ++K Sbjct: 1269 GIPDYLEKDSDGDGI--PDYQE----------------DENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSLVKPVLHET----LSKKHQMPSENPSTKDSDGDGIPDYLEK 1419 HSP 5 Score: 54.299 bits (129), Expect = 1.716e-7 Identity = 60/136 (44.12%), Postives = 74/136 (54.41%), Query Frame = 2 Query: 5 IPDYLDK--DYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLE-DENGDGIPDYLEKDSDGDGIP-DYQEDEDGDGIPDYHKIKQIHSNF 400 IPD+LDK D K+ + +L + + H D DGIPD + D D DG D K+D D DGI + ++D D DGIPD + D NGDGIPD + KDSDGDGIP D+D DGIPD NF Sbjct: 259 IPDFLDKNRDVNNKADFEAILADAARQDH------------DCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNF 382 HSP 6 Score: 50.447 bits (119), Expect = 2.521e-6 Identity = 60/178 (33.71%), Postives = 87/178 (48.88%), Query Frame = 2 Query: 110 TKDSDEDGIPDYLEKDSDGDGIPDYKE-DEDGDGIPDYLEN-----------------DSDGDGIP-------------DYLEDENGDGIPDYLEKDSDGDGIPDY--------QEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK----VQVDKSEDSDGDGIPDNI 514 T+DSD DG +++++D D DGIPD ++ D +GDGIPD E+ DSD DG+P D LED +G+ IPD+L+K+ D + D+ ++D D DGIPD + + F K S G + + D + + DS+GDGIPD I Sbjct: 170 TQDSDWDGDLNHIDEDDDNDGIPDKEDPDSNGDGIPDCREDSSDNKLKLRFKSRQKRLDSDMDGVPDDSDIDDDQDGIPDILEDSDGNDIPDFLDKNRDVNNKADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCI 347
BLAST of SMED30004176 vs. Planmine SMEST
Match: SMESG000054821.1 (SMESG000054821.1) HSP 1 Score: 444.891 bits (1143), Expect = 1.375e-142 Identity = 228/229 (99.56%), Postives = 228/229 (99.56%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 688 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE Sbjct: 1387 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKESIQNEINENKLSMPRIIIRQVLQSLENFFGTDE 1615 HSP 2 Score: 162.925 bits (411), Expect = 3.834e-43 Identity = 109/200 (54.50%), Postives = 124/200 (62.00%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDKSNHRKSACKSKESCGKIIDLKKES 592 GIPDYL+KD G + P E D D DGIPDYL+ DSDGDGIPDYKEDEDGDGIPDYLE DSDGDGIPDY+EDENGDGIPDYLEKDSDGDGIPDY+EDEDGDGIPDY + + G L+KD D ED+DGDGIPD +DK + K + +LK++S Sbjct: 1052 GIPDYLEKDSDGDGI--PDYKE----------------DEDGDGIPDYLQNDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYMEDENGDGIPDYLEKDSDGDGIPDYKEDADGDGIPDYLDKDTESTNIKKESSKNNVLDNLKQDS 1233 HSP 3 Score: 161.384 bits (407), Expect = 1.232e-42 Identity = 107/176 (60.80%), Postives = 119/176 (67.61%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK---VQVDKSEDSDGDGIPDNIDK 520 GIPDYLDKD + ++ K SK + + + +DSD DGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYL+ DSDGDGIPDY EDE+GDGIPDYLEKDSDGDGIPDYQEDE+GDGIPDY L+KD D EDSDGDGIPD +DK Sbjct: 1202 GIPDYLDKDTESTNIKK-----ESSKNNVLDN---LKQDSDGDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLQKDSDGDGIPDYKEDEDGDGIPDYLEKDSDGDGIPDYQEDENGDGIPDY-------------------------LEKDSDGDGIPDFKEDSDGDGIPDYLDK 1344 HSP 4 Score: 148.673 bits (374), Expect = 2.548e-38 Identity = 94/173 (54.34%), Postives = 114/173 (65.90%), Query Frame = 2 Query: 2 GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLEDENGDGIPDYLEKDSDGDGIPDYQEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDKVQVDKSEDSDGDGIPDNIDK 520 GIPDYL+KD G + P E D + DGIPDYLEKDSDGDGIPD+KED DGDGIPDYL+ D+DGDGIP+Y ED +GDGIPDYL+KDSDGDGIPDY+EDEDGDGIPDY + K + ++K + + ++DSDGDGIPD ++K Sbjct: 1287 GIPDYLEKDSDGDGI--PDYQE----------------DENGDGIPDYLEKDSDGDGIPDFKEDSDGDGIPDYLDKDTDGDGIPNYKEDADGDGIPDYLDKDSDGDGIPDYKEDEDGDGIPDYLDKDYKGKSLVKPVLHET----LSKKHQMPSENPSTKDSDGDGIPDYLEK 1437 HSP 5 Score: 54.299 bits (129), Expect = 1.563e-7 Identity = 60/136 (44.12%), Postives = 74/136 (54.41%), Query Frame = 2 Query: 5 IPDYLDK--DYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEKDSDGDGIPDYKEDEDGDGIPDYLENDSDGDGIPDYLE-DENGDGIPDYLEKDSDGDGIP-DYQEDEDGDGIPDYHKIKQIHSNF 400 IPD+LDK D K+ + +L + + H D DGIPD + D D DG D K+D D DGI + ++D D DGIPD + D NGDGIPD + KDSDGDGIP D+D DGIPD NF Sbjct: 277 IPDFLDKNRDVNNKADFEAILADAARQDH------------DCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCIMKDSDGDGIPDHIDTDDDNDGIPDLKDPNHPLFNF 400 HSP 6 Score: 50.8322 bits (120), Expect = 2.017e-6 Identity = 60/178 (33.71%), Postives = 87/178 (48.88%), Query Frame = 2 Query: 110 TKDSDEDGIPDYLEKDSDGDGIPDYKE-DEDGDGIPDYLEN-----------------DSDGDGIP-------------DYLEDENGDGIPDYLEKDSDGDGIPDY--------QEDEDGDGIPDYHKIKQIHSNFTKKITPSASIGKVTKLKKDK----VQVDKSEDSDGDGIPDNI 514 T+DSD DG +++++D D DGIPD ++ D +GDGIPD E+ DSD DG+P D LED +G+ IPD+L+K+ D + D+ ++D D DGIPD + + F K S G + + D + + DS+GDGIPD I Sbjct: 188 TQDSDWDGDLNHIDEDDDNDGIPDKEDPDSNGDGIPDCREDSSDNKLKLRFKSRQKRLDSDMDGVPDDSDIDDDQDGIPDILEDSDGNDIPDFLDKNRDVNNKADFEAILADAARQDHDCDGIPDQYDPDDDNDGFFDKKQDSDRDGILNEYDDDDDNDGIPDSEDPDSNGDGIPDCI 365 The following BLAST results are available for this feature:
BLAST of SMED30004176 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30004176 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30004176 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30004176 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30004176 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30004176 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30004176 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004176 ID=SMED30004176|Name=SMED30004176|organism=Schmidtea mediterranea sexual|type=transcript|length=1307bpback to top protein sequence of SMED30004176-orf-1 >SMED30004176-orf-1 ID=SMED30004176-orf-1|Name=SMED30004176-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=230bp GIPDYLDKDYKGKSLVKPVLHETLSKKHQMPSENPSTKDSDEDGIPDYLEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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