Holocarboxylase synthetase

Overview
NameHolocarboxylase synthetase
Smed IDSMED30004174
Length (bp)5741
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Holocarboxylase synthetase (SMED30004174) t-SNE clustered cells

Violin plots show distribution of expression levels for Holocarboxylase synthetase (SMED30004174) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Holocarboxylase synthetase (SMED30004174) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Holocarboxylase synthetase (SMED30004174) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004174

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30004174 SmedASXL_035267SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30004174 SmedASXL_024661SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30004174 dd_Smed_v4_12353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004174 dd_Smed_v4_12353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004174 dd_Smed_v4_12353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004174 dd_Smed_v4_12353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004174 dd_Smed_v4_12353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004174 dd_Smed_v4_12353_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Human
Match: HLCS (holocarboxylase synthetase [Source:HGNC Symbol;Acc:HGNC:4976])

HSP 1 Score: 97.4413 bits (241), Expect = 1.292e-19
Identity = 43/99 (43.43%), Postives = 69/99 (69.70%), Query Frame = 3 
Query:  756 YFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            Y  +L TK LGK++LF+EV  ++  L++ ++ Q      +++I+  QT GKGRG N W+SP+GCA+ T LI I +RS+LG  +  +QH+M+V++VEA+ 
Sbjct:  456 YRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVR 554
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Human
Match: HLCS (holocarboxylase synthetase [Source:HGNC Symbol;Acc:HGNC:4976])

HSP 1 Score: 97.4413 bits (241), Expect = 1.292e-19
Identity = 43/99 (43.43%), Postives = 69/99 (69.70%), Query Frame = 3 
Query:  756 YFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            Y  +L TK LGK++LF+EV  ++  L++ ++ Q      +++I+  QT GKGRG N W+SP+GCA+ T LI I +RS+LG  +  +QH+M+V++VEA+ 
Sbjct:  456 YRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVR 554
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Human
Match: HLCS (holocarboxylase synthetase [Source:HGNC Symbol;Acc:HGNC:4976])

HSP 1 Score: 97.4413 bits (241), Expect = 1.292e-19
Identity = 43/99 (43.43%), Postives = 69/99 (69.70%), Query Frame = 3 
Query:  756 YFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            Y  +L TK LGK++LF+EV  ++  L++ ++ Q      +++I+  QT GKGRG N W+SP+GCA+ T LI I +RS+LG  +  +QH+M+V++VEA+ 
Sbjct:  456 YRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVR 554
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Celegans
Match: bpl-1 (Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Acc:Q9BI98])

HSP 1 Score: 71.633 bits (174), Expect = 3.532e-12
Identity = 34/113 (30.09%), Postives = 70/113 (61.95%), Query Frame = 3 
Query:  708 DKNPDFQEIVSFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERI-VQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEA 1043
            +++ D     +F  N YF  +++K +G++VL  +V +++ D++E +   + + + +V+I+  Q +G+GRG N+++ P G AM+     I  +SR+   + ++QHI  V++VEA
Sbjct:  279 NRDSDAGTSKNFDFNLYFEQIHSK-IGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEA 390
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Celegans
Match: bpl-1 (Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Acc:Q9BI98])

HSP 1 Score: 71.633 bits (174), Expect = 3.532e-12
Identity = 34/113 (30.09%), Postives = 70/113 (61.95%), Query Frame = 3 
Query:  708 DKNPDFQEIVSFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERI-VQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEA 1043
            +++ D     +F  N YF  +++K +G++VL  +V +++ D++E +   + + + +V+I+  Q +G+GRG N+++ P G AM+     I  +SR+   + ++QHI  V++VEA
Sbjct:  279 NRDSDAGTSKNFDFNLYFEQIHSK-IGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEA 390
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Celegans
Match: bpl-1 (Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Acc:Q9BI98])

HSP 1 Score: 71.633 bits (174), Expect = 4.260e-12
Identity = 34/113 (30.09%), Postives = 70/113 (61.95%), Query Frame = 3 
Query:  708 DKNPDFQEIVSFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERI-VQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEA 1043
            +++ D     +F  N YF  +++K +G++VL  +V +++ D++E +   + + + +V+I+  Q +G+GRG N+++ P G AM+     I  +SR+   + ++QHI  V++VEA
Sbjct:  353 NRDSDAGTSKNFDFNLYFEQIHSK-IGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEA 464
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Celegans
Match: bpl-1 (Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Acc:Q9BI98])

HSP 1 Score: 71.2478 bits (173), Expect = 8.411e-12
Identity = 34/106 (32.08%), Postives = 66/106 (62.26%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERI-VQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFL 1055
            F  N YF  +++K +G++VL  +V +++ D++E +   + + + +V+I+  Q +G+GRG N+++ P G AM+     I  +SR+   + ++QHI  V++VEA   L
Sbjct:  947 FDFNLYFEQIHSK-IGQVVLLVDVATTTMDIIESVNAGIPSLESVVVIANRQISGRGRGGNEFLCPRGMAMFNFSFSISKKSRIAKHLPILQHIFCVALVEAARNL 1051
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Fly
Match: Hcs (gene:FBgn0037332 transcript:FBtr0078683)

HSP 1 Score: 75.485 bits (184), Expect = 4.842e-13
Identity = 35/103 (33.98%), Postives = 66/103 (64.08%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIVQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            F + DYF +L T+++G++V+++ V+SSS  L   I  L     + ++   QT+G GR +N+W+SP GCAM++  + + + S L + + L+QH++  +IV ++ 
Sbjct:  767 FSTVDYFDNLKTEHIGRLVIYAPVVSSSMHL---INNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLR 866
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Fly
Match: Hcs (gene:FBgn0037332 transcript:FBtr0336769)

HSP 1 Score: 75.485 bits (184), Expect = 4.944e-13
Identity = 35/103 (33.98%), Postives = 66/103 (64.08%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIVQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            F + DYF +L T+++G++V+++ V+SSS  L   I  L     + ++   QT+G GR +N+W+SP GCAM++  + + + S L + + L+QH++  +IV ++ 
Sbjct:  719 FSTVDYFDNLKTEHIGRLVIYAPVVSSSMHL---INNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLR 818
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Fly
Match: Hcs (gene:FBgn0037332 transcript:FBtr0345254)

HSP 1 Score: 75.485 bits (184), Expect = 5.172e-13
Identity = 35/103 (33.98%), Postives = 66/103 (64.08%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIVQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            F + DYF +L T+++G++V+++ V+SSS  L   I  L     + ++   QT+G GR +N+W+SP GCAM++  + + + S L + + L+QH++  +IV ++ 
Sbjct:  725 FSTVDYFDNLKTEHIGRLVIYAPVVSSSMHL---INNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLR 824
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Fly
Match: Hcs (gene:FBgn0037332 transcript:FBtr0345253)

HSP 1 Score: 75.485 bits (184), Expect = 5.194e-13
Identity = 35/103 (33.98%), Postives = 66/103 (64.08%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIVQLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            F + DYF +L T+++G++V+++ V+SSS  L   I  L     + ++   QT+G GR +N+W+SP GCAM++  + + + S L + + L+QH++  +IV ++ 
Sbjct:  773 FSTVDYFDNLKTEHIGRLVIYAPVVSSSMHL---INNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLR 872
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Xenopus
Match: en2 (engrailed homeobox 2 [Source:Xenbase;Acc:XB-GENE-853108])

HSP 1 Score: 90.8929 bits (224), Expect = 1.141e-17
Identity = 45/110 (40.91%), Postives = 71/110 (64.55%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-----QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFL 1055
            F    Y  SL T  LGKIVLF+EV +++F+L+E ++     ++G    ++ I+  QT GKGRG N W+SP GCA+ T  I I + S LG  ++ +QH+M++++VE++  L
Sbjct:  420 FSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMG----LIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSL 525
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Xenopus
Match: en2 (engrailed homeobox 2 [Source:Xenbase;Acc:XB-GENE-853108])

HSP 1 Score: 90.8929 bits (224), Expect = 1.326e-17
Identity = 45/110 (40.91%), Postives = 71/110 (64.55%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-----QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFL 1055
            F    Y  SL T  LGKIVLF+EV +++F+L+E ++     ++G    ++ I+  QT GKGRG N W+SP GCA+ T  I I + S LG  ++ +QH+M++++VE++  L
Sbjct:  579 FSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMG----LIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSL 684
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Mouse
Match: Hlcs (holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) [Source:MGI Symbol;Acc:MGI:894646])

HSP 1 Score: 93.5893 bits (231), Expect = 1.215e-19
Identity = 48/132 (36.36%), Postives = 80/132 (60.61%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLCWIDVSVNSALISDKNLIFKNP*EIY 1133
            F    Y  +L T  LGK++LF+EV S++  L++ ++ ++     ++ I+  QT GKGRG N W+SP+GCA+ T L+ I +RS+LG  +  +QH+M++++VEA+  +   +         D NL  K P +IY
Sbjct:   52 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPGYE---------DINLRVKWPNDIY 174
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Mouse
Match: Hlcs (holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) [Source:MGI Symbol;Acc:MGI:894646])

HSP 1 Score: 93.9745 bits (232), Expect = 1.235e-18
Identity = 48/132 (36.36%), Postives = 80/132 (60.61%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLCWIDVSVNSALISDKNLIFKNP*EIY 1133
            F    Y  +L T  LGK++LF+EV S++  L++ ++ ++     ++ I+  QT GKGRG N W+SP+GCA+ T L+ I +RS+LG  +  +QH+M++++VEA+  +   +         D NL  K P +IY
Sbjct:  447 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPGYE---------DINLRVKWPNDIY 569
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Mouse
Match: Hlcs (holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) [Source:MGI Symbol;Acc:MGI:894646])

HSP 1 Score: 93.9745 bits (232), Expect = 1.235e-18
Identity = 48/132 (36.36%), Postives = 80/132 (60.61%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLCWIDVSVNSALISDKNLIFKNP*EIY 1133
            F    Y  +L T  LGK++LF+EV S++  L++ ++ ++     ++ I+  QT GKGRG N W+SP+GCA+ T L+ I +RS+LG  +  +QH+M++++VEA+  +   +         D NL  K P +IY
Sbjct:  447 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPGYE---------DINLRVKWPNDIY 569
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Mouse
Match: Hlcs (holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) [Source:MGI Symbol;Acc:MGI:894646])

HSP 1 Score: 93.2041 bits (230), Expect = 2.486e-18
Identity = 48/132 (36.36%), Postives = 80/132 (60.61%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLCWIDVSVNSALISDKNLIFKNP*EIY 1133
            F    Y  +L T  LGK++LF+EV S++  L++ ++ ++     ++ I+  QT GKGRG N W+SP+GCA+ T L+ I +RS+LG  +  +QH+M++++VEA+  +   +         D NL  K P +IY
Sbjct:  594 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPGYE---------DINLRVKWPNDIY 716
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Mouse
Match: Hlcs (holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) [Source:MGI Symbol;Acc:MGI:894646])

HSP 1 Score: 71.633 bits (174), Expect = 2.732e-14
Identity = 35/88 (39.77%), Postives = 55/88 (62.50%), Query Frame = 3 
Query:  870 IVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLCWIDVSVNSALISDKNLIFKNP*EIY 1133
            ++ I+  QT GKGRG N W+SP+GCA+ T L+ I +RS+LG  +  +QH+M++++VEA+  +   +         D NL  K P +IY
Sbjct:   10 LIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPGYE---------DINLRVKWPNDIY 88
[Close]
BLAST of Holocarboxylase synthetase vs. UniProt/SwissProt
Match: sp|P50747|BPL1_HUMAN (Biotin--protein ligase OS=Homo sapiens OX=9606 GN=HLCS PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 6.207e-19
Identity = 43/99 (43.43%), Postives = 69/99 (69.70%), Query Frame = 3 
Query:  756 YFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAIN 1049
            Y  +L TK LGK++LF+EV  ++  L++ ++ Q      +++I+  QT GKGRG N W+SP+GCA+ T LI I +RS+LG  +  +QH+M+V++VEA+ 
Sbjct:  456 YRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGKGRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVR 554
[Close]
BLAST of Holocarboxylase synthetase vs. UniProt/SwissProt
Match: sp|Q920N2|BPL1_MOUSE (Biotin--protein ligase OS=Mus musculus OX=10090 GN=Hlcs PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 8.636e-18
Identity = 48/132 (36.36%), Postives = 80/132 (60.61%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLCWIDVSVNSALISDKNLIFKNP*EIY 1133
            F    Y  +L T  LGK++LF+EV S++  L++ ++ ++     ++ I+  QT GKGRG N W+SP+GCA+ T L+ I +RS+LG  +  +QH+M++++VEA+  +   +         D NL  K P +IY
Sbjct:  447 FSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGKGRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSIPGYE---------DINLRVKWPNDIY 569
[Close]
BLAST of Holocarboxylase synthetase vs. UniProt/SwissProt
Match: sp|F4I4W2|HCS2_ARATH (Biotin--protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=HCS2 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.853e-7
Identity = 34/111 (30.63%), Postives = 62/111 (55.86%), Query Frame = 3 
Query:  738 SFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIVQLGNFQPI----VIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLC 1058
            SF  + + +S+ T   G+ +++S  LSS+ D++       NF  I    V +S +Q  G+GR  N W SP GC MY+  + ++     G  + LIQ+++++++ EA+  +C
Sbjct:   57 SFNLSLFMNSISTHRFGRFLIWSPYLSSTHDVVSH-----NFSEIPVGSVCVSDIQLKGRGRTKNVWESPKGCLMYSFTLEMED----GRVVPLIQYVVSLAVTEAVKDVC 158
[Close]
BLAST of Holocarboxylase synthetase vs. UniProt/SwissProt
Match: sp|Q9SL92|HCS1_ARATH (Biotin--protein ligase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HCS1 PE=1 SV=2)

HSP 1 Score: 57.3806 bits (137), Expect = 8.422e-7
Identity = 35/123 (28.46%), Postives = 67/123 (54.47%), Query Frame = 3 
Query:  738 SFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIVQLGNFQPI----VIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFLC------WIDVSV 1076
            SF  + + +S+ T   G+ +++S  LSS+ D++       NF  +    V ++ +Q  G+GR  N W SP GC MY+  + ++     G  + LIQ+++++++ EA+  +C      +IDV +
Sbjct:   95 SFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSH-----NFSELPVGSVCVTDIQFKGRGRTKNVWESPKGCLMYSFTLEMED----GRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKI 208
[Close]
BLAST of Holocarboxylase synthetase vs. TrEMBL
Match: A0A355ABF2 (Uncharacterized protein OS=Flavobacteriaceae bacterium OX=1871037 GN=DDZ39_05755 PE=4 SV=1)

HSP 1 Score: 476.093 bits (1224), Expect = 4.814e-133
Identity = 236/633 (37.28%), Postives = 383/633 (60.51%), Query Frame = -1 
Query: 2511 IFPWAIVMLFIFCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKVNGKIIKQSVVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPV-LMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNGEGKVNYEYLLTPDHFRKATYKTMKKIWGKFMFLGQACAGLIGFYVVIITIKVIMSQILSAHRIYVISGWSWKIIIGCFPFLARFFITHHQYREMTSVRAQ 4406
            +F + I+ML +F     +   + AYDC   ++G  +S++D  ECP+AYP + +   +  Y++YQE  L  +  +EC   R   + +CG  SH+++IK  ++   + +   NC  A + + +++  ++ LKAK    I+E ++  G I  DGTCE   Y   GK IKQ++VIE Y   +++ E  FD +T ++ +  +C+ + + C  G++ +++D++   CNL  LKT+NF  +  + F  +     +N +     R+        TPV L++   +D++R ++K+   KCGQ VY TNY  + VS + V EA  KI   +I L  YFNNK DYLYH     +E++YREMI+NDCKLN+EILR +MA+ +TNPN+ AP++    GVF+R+ GE L ++KCK + VKI     CT+ELPV    K  ++EP+TR+LL +  K K ++C+ L +P Y+L+ ++WI  P    +  P + +LTNL   V F+ L  + + G Y  + +E+A + LL+P  ++R+++E+++T L +G  + NYE LL+P+HF KA    MKK+WG+F+  GQA AGL+G Y V+I +K  + Q+ S + +Y I G+SWK+I+G  P LAR FI+   + ++  V  +
Sbjct:  372 VFNFVILMLLLFSL--PKGKAIKAYDCDNAKLGTQFSLLDAEECPEAYPNQFEKRFNRIYHIYQERGLIYSAAKECTVTRRRSIRWCGHHSHSAIIKQPSMIEYLNIGHRNCEDAFRLKEIRLADKLILKAKPGILIQEDIIKVGSIAFDGTCEGGEYWYQGKKIKQALVIETYQITIKKSEVAFDPDTNEMMSRSYCSAKSNFCFDGKTSIIYDIRKQECNLVFLKTVNFDVIRGKIFDNNLFIDKLNSRPKKIYRTEKTINSHITPVVLIANKTSDAIRLVRKDQVVKCGQSVYLTNYDRIVVSTVRVKEAIIKISKKEINLAVYFNNKADYLYHHQLRQIEDLYREMIINDCKLNREILRTKMALIVTNPNIAAPVIPLGKGVFSRVLGEVLQTFKCKQVEVKINISSDCTHELPVIYKNKFMYLEPVTRLLLPDVIKVKKIKCSPLFSPAYKLSDNTWIVTPSLTPIAPPKRFQLTNLRNAVKFSRLKDLMKSGLYDKKAMEDARKYLLYPQVRDRILTEIVETSLGDG-NRPNYELLLSPNHFEKAAKNVMKKVWGRFLIFGQAIAGLMGIYYVVIFLKTFIEQVTSTYELYKIMGFSWKLILGIIPCLARPFISKKLHDKINDVEKK 1001
[Close]
BLAST of Holocarboxylase synthetase vs. TrEMBL
Match: A0A151IFZ0 (Uncharacterized protein OS=Cyphomyrmex costatus OX=456900 GN=ALC62_09092 PE=4 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 3.389e-39
Identity = 142/615 (23.09%), Postives = 263/615 (42.76%), Query Frame = -1 
Query: 2589 IVMLFIFCTITTQATGMTAYDCSQTEMG-KIYSVVDMAECP--DAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKVNGKIIKQSVVIEEYL-TELREFEGVFDDETGK--LQTHPFCNMRQSNCQTGESILVF--DVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYV---SKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIAL-PDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNG--EGKVNYEYLLTPDHFRKATYKTMKKIWGKFMFLGQACAGLIGFYVVIITIKVIMSQILSAHRIYVISGWSWKII 4391
            I+++ IF  +T    G+  YDC          S++D+  C   D  P+    E +V   + Q SD  +    +C  +    + +CG  SH S++         E+    C    +T VL+I   +    K N     +  + G    DG C    Y  +G    +SVV++  +   LR  E     + G   + +  FC      C   +    F   +  + C+                     DN++I Y+   T R  P     T  V   T    +    K    + CG  +  T +  +++    K    + +TK+  +++ +  Y N+K  Y+    +  +  +YR ++   C L K++L+N + +A   P+  A  ++ E G  A + GEAL+  KC  +  K+   +KC  ELPV+      F++P +RIL    T   I  CN+LL  +Y+L    W  L P    +  P  ++  N      +TS + +A  G Y +E+++    +++FP ++  +++ +    + N    G ++   LL  D   +      KK+W  F+  G A AG++  +++I  +++I+  ++  + ++ + GWS  +I
Sbjct:    4 IIIVLIFAPLTQ---GLIGYDCGGEGFNISTLSLLDVGNCDLEDVEPR----EEEVYIQLMQMSDYDKVPAIQCRIEVNRVIHYCGMHSHISVVHNGRREYFQEIGERACRRVHETGVLQIGNAVMDLLKNNATNYRSATLAGTATVDGRCAGAQY-TDGYGSWESVVVQATIKITLRTLELSVKRKLGHVIMPSGTFCKTSDKYCIDADGTETFWSPIVVDNCHF--------------------DNYDILYEGIAT-RLVPANNYTTPIVYTVTTQETTFALTKTIDMDVCGYKLSQTEHPKLFILQTQKGRTFKTRTKVSVDNLDIFFYVNSKFVYVEKHIKKQLTQLYRNLMEQKCALEKQVLKNALTLASIAPDETAYRIMREPGYTAVLSGEALHLVKCVPVECKLRHVDKCYTELPVTHQNTSYFLQPRSRILTKTGT---IRDCNQLLPVMYKL-HGIWFRLGPKPVEITAPAVIQPLNHPNW-QYTSSSSLATSGIYNTEDLDRLRAHIMFPVERPSIVNTLARGAMGNAIPAGSISLSTLLDEDSLNRIADSAGKKVWRGFVTFGSASAGILAIFIIIRVVELIIDTMIHGYALHTMYGWSLHLI 584
[Close]
BLAST of Holocarboxylase synthetase vs. TrEMBL
Match: A0A0L7QJ86 (Uncharacterized protein OS=Habropoda laboriosa OX=597456 GN=WH47_08632 PE=4 SV=1)

HSP 1 Score: 165.622 bits (418), Expect = 3.074e-38
Identity = 148/616 (24.03%), Postives = 275/616 (44.64%), Query Frame = -1 
Query: 2553 GMTAYDCSQTEMG-KIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINP-EIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKV-----NGKIIKQSVVIEEYLTELREFEGVFDDETGKL--QTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNK-CGQMVYATNYRGVYV---SKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALP-DYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVIS----EMIDTILPNGEGKVNYEYLLTPDHFRKATYKTMKKIWGKFMFLGQACAGLIGFYVVIITIKVIMSQILSAHRIYVISGWSWKIIIGCFPFLARFFI 4346
             + AYDC+   +     S+  + EC +     LK EN     + Q ++ +  +V +C  K    +++CG +SHTS+++       +E+  E C    +T  ++ +  +I    ++N     ++ + G I  DG C    Y       N  +++ SV I      LR F       T  L   +   C + + +C+  E    F   T V +  H                  D +++ Y+      S P  T  + P + S    D+   +   +T   CG  +  T +  +++   SK    ++K+KI  N++ L  Y N+K  Y+    +  +  +Y  +I   C L +++LRN +A+A   P+ +A  ++ + G  A   GE ++  KC  +   I   + C NELPV++     FM+P  RIL+   T PK  +CN LL  +Y+L  + +   P     +  PD   LT   +  ++ +   +A  G Y  + I+E   ++LFP +K   ++      +  ++P  +G V  + LL     ++ T  T K++W  F   G   AG +G Y++   IK+I+  +++ + ++ + GWS  ++   +  LA+  +
Sbjct:   18 ALVAYDCAGHSLNITTLSLTGIGEC-EVPRSDLKSENQF-IQLLQLTEYKNTRVIQCKVKVDRTIYYCGMYSHTSVVQGGQREYLMEITSELCNQLHRTGQIQFSANDIITGLRINTTSTRSLTLAGTITLDGRCTGAQYSDPFGTWNNVVVQASVTIT-----LRSFYAPIKLGTSHLILPSGRHCLVTEESCEDAEGEATFWTAT-VEDNCHF-----------------DRYDVLYEGMANKVSPPDST--SGPTMFSLTSQDTTFALTTTSTLPLCGYFLLKTEHPKLFILATSKENTFKSKSKIEVNNLDLLTYVNSKFVYVERHIKTQLTELYANIINQRCALEQQVLRNALALANIQPDELALAIMKQPGYMAVPAGEVVHLVKCVPVECTIRHTQFCYNELPVTVRNTSYFMKPKNRILVRTAT-PK--ECNELLPTMYQLHGNWYKMNPKPMESLPPPDIQPLTTPTW--HYKNPANLATSGIYTQQQIDELRDHILFPIEKPATLNIIARGAMGGVIP--QGVVQLDNLLDEAALQRITESTAKRMWNFFTAFGSISAGCMGVYLICRIIKLILDTLINGYALHAVYGWSLHLLGAMWNSLAQLLL 599
[Close]
BLAST of Holocarboxylase synthetase vs. TrEMBL
Match: A0A0B5KJV8 (Glycoprotein OS=Tacheng Tick Virus 4 OX=1608086 GN=G PE=4 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.353e-37
Identity = 156/647 (24.11%), Postives = 275/647 (42.50%), Query Frame = -1 
Query: 2511 TITTQATGMTAYDCSQTEMGKI-YSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKVNGKIIKQSVVIEEYLTELREFEGVFDDETGKLQTHPF----CNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTP----------VWTRKTTP--VLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIY------REMILN----DCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIAL-PDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNGEGKVNYEYLLTPDHFRKATYKTMKKIWGKFMFLGQACAGLIGFYVVIITIKVIMSQILSAHRIYVISGWSWKIIIGCFPFLARFFITHHQYREMTSVRAQ 4367
            T T  +  +TA+DC+QT +  + Y +  +AECPD   + +  E      V Q+ +    +V  C    T  ++ CG  SHTS++      +P++V  + C       V K      ++ K N     TV V G +  DGTC      +NG+    SVV  E + ++R  + +   + G      F    C +   +C        FDV      L     ++ K             +++ Y  +  I  T           V  R +TP  +    L  DS           CG  V  T Y G+YV+   ++++        I    Y N  +D + +    ++   Y      REM++N     C L++E L NR+ +  + P+    +L +E GVF R+ GE ++  +C A+ V + + + C+ E+P+   G+  +MEP TRI+  E T     +C  L+  ++R   + W+   P Y     P K+   +   L +F  L   A+ G Y   ++++    +LF  K++ + + ++  +      K +   +L    + +     +++IWGKF  LG+   GL+G YV  + +++    IL    IY   G + K+++G +      F+     RE    R +
Sbjct:   24 TTTRGSAVVTAFDCNQTTLDTVTYDLTRVAECPDISSRDIS-ETTRLVQVIQQKEYGVLRVYRCHVTVTPQIWHCGMHSHTSIVSAPFSAQPLDVSEQECRLMQMDGVYKTATGAIVRLKRNTTSLHTVDVAGKVHPDGTCVGGSSHINGQPY-YSVVHREAI-QIRLEDKLVHYKIGDTIVVGFGGVTCRLADISCHD------FDVGRMYWTLDAAGCLDGK-------------YDVLYTGDAAIIHTAHLGPNKEYMRVLRRASTPFQIFTPLLGDDS----------ACGVEVRTTPYAGLYVA---LYQSSPPFPIGPIT---YSN--VDVVKYLEAKMVTFAYGSALTAREMVVNLERQQCSLHRETLLNRLHIVASTPSSATLLLDNEPGVFGRVAGEVVHLLRCPAVEVTLRRADHCSLEIPIRHLGRDVYMEPRTRIITTEYTH---RECGPLVPVMFRHG-NQWVQFSPQYSPAPEPKKIHPMS-PTLPSFERLVAYARGGIYDPRDLQQLRDFILFSHKRDAIETNIVAGLSGQSAPKYDLTQVLNSGGWEELARDRLQQIWGKFQGLGKIVNGLVGIYVCFVIVRITFGLILRTFTIYNTHGCT-KLMLGVWSSALTHFLLSLAGRERQEPREE 624
[Close]
BLAST of Holocarboxylase synthetase vs. TrEMBL
Match: A0A151J7N2 (Uncharacterized protein OS=Trachymyrmex cornetzi OX=471704 GN=ALC57_08270 PE=4 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 2.718e-37
Identity = 134/556 (24.10%), Postives = 244/556 (43.88%), Query Frame = -1 
Query: 2589 KVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKA-KVNHRIRETVVVKGVIQQDGTCE*----ELYKVNGKIIKQSVVIEEYLTELREFEGVFDDETG--KLQTHPFCNMRQSNCQTGESILVF--DVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNK--CGQMVYATNYRGVYVSKMMVHEA---KTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPD-KLELTNLEYLV----NFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTIL--PNGEGKVNYEYLLTPDHFRKATYKTMKKIWGKFMFLGQACAGLIGFYVVIITIKVIMSQILSAHRIYVISGWSWKII 4193
            +V +C      F+++CG  SH S ++       +E+  E C     T +   +    +   KVN       +  G    DG C      +LY     +I Q          L  ++   + ET   +L++   C    + C   +    F   + T+ C   H     +  L E       D F  +                  P ++ +L T  + F       +  CG  +  T +  + + +    E+   K ++ T ++ +  Y N+K  Y+    ++ M  +YR+++   C L +++L+  +++AI +P+  A  ++   G  A I GE ++  KC  + VKI   ++C +ELPV    +  F+ P T IL+   T+   + CNR++ P++ L  D W     YR   TP+  L  T ++ +     ++T+   +A  G Y+  ++E    +++FP++K  +++ M   +   P     ++  +LL     +K T  T KKIWG F+  G A AG+IG Y  I T+K+I+  I+  + I+ I GWS  +I
Sbjct:   71 EVIQCKVSINRFIYYCGMHSHLSTVRNAQAEYILEITAEQCKKMHLTGIFSFDIHNYIYGLKVNQTTMRPTIFAGSANSDGRCSGAQYSDLYGAWDNVIVQGTTT----ITLTSYQTSINLETNQIRLKSGTICPYTDATCMDIDGGHTFWKTLPTDHCKFNH-----YDVLYEGYANRMVDTFFEH------------------PQIVYSLSTQDITFALTRTGEEPVCGYTLIKTEHPKLLILETKKGESFTTKRRLSTENLDIFTYINSKFVYVEKHIRSQMNLLYRDVLKQRCTLEQQVLKGALSLAINSPDEFAYQIMKGPGYMAVISGEVVHIIKCTPVDVKIQHVKECYSELPVQKGNETYFLSPRTHILIKTGTQ---ISCNRVIPPMFFL-NDGW-----YRMTPTPEHSLPPTIMKPMTKPTWHYTNPGSLAASGIYSDTDLENLRDHIMFPAEKGGILNAMARGVKGEPIVSQGISLSHLLDESSIQKITENTWKKIWGNFISFGNASAGIIGIYFCIRTVKLIIDTIIHGYAIHTIYGWSLHLI 590
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Cavefish
Match: hlcs (holocarboxylase synthetase [Source:NCBI gene;Acc:103022047])

HSP 1 Score: 91.2781 bits (225), Expect = 7.205e-18
Identity = 38/106 (35.85%), Postives = 69/106 (65.09%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFL 1055
            F    Y   L ++ LG+ +L+++V  ++ DL+E ++ ++     ++ ++  QT GKGRG N W+SPLGCAM+T  + +++ SRLG  +  +QH+ A+++VEA+  L
Sbjct:  581 FSMQTYTQHLQSRRLGRTLLYTDVTPTTMDLLEGLMLKVPEDMGLIAVAARQTKGKGRGGNAWLSPLGCAMFTLHLQVEVSSRLGQRIPFLQHLAALAVVEAVRTL 686
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Cavefish
Match: hlcs (holocarboxylase synthetase [Source:NCBI gene;Acc:103022047])

HSP 1 Score: 91.2781 bits (225), Expect = 7.786e-18
Identity = 38/106 (35.85%), Postives = 69/106 (65.09%), Query Frame = 3 
Query:  741 FKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFL 1055
            F    Y   L ++ LG+ +L+++V  ++ DL+E ++ ++     ++ ++  QT GKGRG N W+SPLGCAM+T  + +++ SRLG  +  +QH+ A+++VEA+  L
Sbjct:  628 FSMQTYTQHLQSRRLGRTLLYTDVTPTTMDLLEGLMLKVPEDMGLIAVAARQTKGKGRGGNAWLSPLGCAMFTLHLQVEVSSRLGQRIPFLQHLAALAVVEAVRTL 733
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Sea Lamprey
Match: HLCS (holocarboxylase synthetase [Source:HGNC Symbol;Acc:HGNC:4976])

HSP 1 Score: 87.4261 bits (215), Expect = 1.726e-17
Identity = 57/190 (30.00%), Postives = 96/190 (50.53%), Query Frame = 3 
Query:  513 IQCHHGD--------LYVFSENHESISSYLESIKLSDPLLNKFQYNSLEFNVNLSDNQIFDSNKPFKLLTNINDKNPDFQEIVSFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSR--LGNSMSLIQHIMAVSIVEAIN 1049
            + C  GD        L+        + S LE  +LS+  +   +  SL F  +L+D     +     LL  + +  PD      F    Y   L T+ LG++VL  EV+ S+ +L++ +   L +   ++ I+  Q  GKGRG N W+SP+GCAM+T  +P+ +RS   LG  +  +QH++A+++VE + 
Sbjct:  283 LNCGPGDAPTLTPAILWALEPVRLPLGSALEP-RLSESGVLASRRVSLRFVRSLADG----AGATPTLLPVVTEAAPDLL-TPHFDLEAYRGMLRTRLLGRLVLHCEVVGSTMELLDGVTPHLPDDVGLIAIATRQIRGKGRGGNAWLSPVGCAMFT--LPLVVRSDSCLGQHLPFLQHLVALAVVEGVR 464
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Yeast
Match: BPL1 (Biotin:apoprotein ligase; covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon; human homolog HLCS can complement yeast BPL1 mutant [Source:SGD;Acc:S000002300])

HSP 1 Score: 53.5286 bits (127), Expect = 3.275e-7
Identity = 31/126 (24.60%), Postives = 68/126 (53.97%), Query Frame = 3 
Query:  690 LLTNINDKNPDFQEIVSFKSNDYFSSLYTK-YLGKIVLFSEVLSSSFDLMERIVQLGNFQP---IVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGN---SMSLIQHIMAVSIVEAI 1046
            +   +++  P FQ   +F   +YF  L  +  +G ++L+ EV++S+  ++     L +  P   ++ +  +Q +G+GRG N WI+P G    T ++ + ++S + N   S+  +Q++  ++  +AI
Sbjct:  348 IFPGVDEDIPPFQYTPNFDMKEYFKYLNVQNTIGSLLLYGEVVTSTSTILNNNKSLLSSIPESTLLHVGTIQVSGRGRGGNTWINPKGVCASTAVVTMPLQSPVTNRNISVVFVQYLSMLAYCKAI 473
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Nematostella
Match: EDO41056 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5A7])

HSP 1 Score: 62.7734 bits (151), Expect = 1.747e-10
Identity = 22/59 (37.29%), Postives = 42/59 (71.19%), Query Frame = 3 
Query:  870 IVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAI 1046
            +++++  Q +GKGRG N W+SP+GC M++  + I+  + LG+ +  +QH+ ++S VEA+
Sbjct:    6 VIVVAKQQISGKGRGGNAWLSPVGCMMFSLEVKIRFSTELGSRLPFLQHLASLSFVEAV 64
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Medaka
Match: hlcs (holocarboxylase synthetase [Source:NCBI gene;Acc:101169801])

HSP 1 Score: 85.5001 bits (210), Expect = 4.057e-16
Identity = 42/115 (36.52%), Postives = 68/115 (59.13%), Query Frame = 3 
Query:  726 QEIVSFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-----QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFL 1055
            QE   F    Y   L +  LG  VL++EV +S+ DL+E +       +G    ++ I+  Q  G+GRG N W+SPLGCAM+T  + +++ S+LG  +  +QH+ A+++VEA+  L
Sbjct:  636 QEWPHFSFETYRKHLKSSLLGNTVLYAEVTTSTMDLLEGMTLHSPKDVG----LIAIAARQIQGRGRGGNAWLSPLGCAMFTLQVQVELSSKLGQRIPFLQHLAALAVVEAVCTL 746
[Close]
BLAST of Holocarboxylase synthetase vs. Ensembl Medaka
Match: hlcs (holocarboxylase synthetase [Source:NCBI gene;Acc:101169801])

HSP 1 Score: 85.5001 bits (210), Expect = 4.544e-16
Identity = 42/115 (36.52%), Postives = 68/115 (59.13%), Query Frame = 3 
Query:  726 QEIVSFKSNDYFSSLYTKYLGKIVLFSEVLSSSFDLMERIV-----QLGNFQPIVIISYVQTAGKGRGSNKWISPLGCAMYTCLIPIQIRSRLGNSMSLIQHIMAVSIVEAINFL 1055
            QE   F    Y   L +  LG  VL++EV +S+ DL+E +       +G    ++ I+  Q  G+GRG N W+SPLGCAM+T  + +++ S+LG  +  +QH+ A+++VEA+  L
Sbjct:  576 QEWPHFSFETYRKHLKSSLLGNTVLYAEVTTSTMDLLEGMTLHSPKDVG----LIAIAARQIQGRGRGGNAWLSPLGCAMFTLQVQVELSSKLGQRIPFLQHLAALAVVEAVCTL 686
[Close]
BLAST of Holocarboxylase synthetase vs. Planmine SMEST
Match: SMESG000080881.1 (SMESG000080881.1)

HSP 1 Score: 1187.56 bits (3071), Expect = 0.000e+0
Identity = 570/622 (91.64%), Postives = 589/622 (94.69%), Query Frame = -1 
Query: 2760 LEIGAQVIAETDNLENSIEGGVRISHEYFIEWVKKNGGWKKLNNDLTVNESDEKKLPYKPLNGKDSPKLRKKMIFPWAIVMLFIFCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKVNGKIIKQSVVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNGEGKVNYEYLLTPDHFRK 4625
            LEIGA  IAETDNLEN I+   R S+EYF++WVK NGGWKKLNNDLTVNE DEKKLPYKPLNGKDSPKLRKKMIFPWAIVMLFI CTITTQATGMTAYDCSQTEMGKIYSVVDMAECP+ YPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAA +TE LKINPEIKLKAKVN RIRETVVVKGVIQQDGTCE E YKVNGKIIKQSVVIEEYLT+LREFEGVFDD+TGKLQTHPFCNMRQSNCQTGESILVFDVKT++CNLAHLKTM FKELS EQFRES DNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTD MRFIKKEATNKCGQMVYATNY+GVYVSKMMV EAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKA+TVKIMKYEKCTNELPVSINGKMRFMEPITRILLDE TKPK LQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLN+MAQMGFYASE+IEEA RNLLFPSKKERVISEMIDTILPNGE +    + +T   +R+
Sbjct: 2358 LEIGAHAIAETDNLENLIDVAARTSNEYFMKWVKNNGGWKKLNNDLTVNEGDEKKLPYKPLNGKDSPKLRKKMIFPWAIVMLFICCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPEVYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAAFQTEELKINPEIKLKAKVNQRIRETVVVKGVIQQDGTCEGEPYKVNGKIIKQSVVIEEYLTKLREFEGVFDDKTGKLQTHPFCNMRQSNCQTGESILVFDVKTDICNLAHLKTMTFKELSGEQFRESLDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDGMRFIKKEATNKCGQMVYATNYKGVYVSKMMVQEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAVTVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEHTKPKFLQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNRMAQMGFYASEDIEEAQRNLLFPSKKERVISEMIDTILPNGEAR----FFITHHQYRE 2975

HSP 2 Score: 535.413 bits (1378), Expect = 1.328e-155
Identity = 295/385 (76.62%), Postives = 310/385 (80.52%), Query Frame = -3 
Query: 4624 GKQ--SLK*ENMNLEDDEFLIDCGFIPGNKNKLQVACMISEEVAEKSDSKEITRDLRDALSDWNGVKITEKLKLLDKIELQENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDCGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKXXXXXXXXXXXDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNV----KTKVDEIFSNKE-------IQVEHLEKQEIFEAPRGEINSEIIVSKPRTLYEIKQSLVKIEQEVDETKIILMGLIKAVYPTFEIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGIDKEVMLTSLTYMVF 5739
            G+Q   +K +N  LEDDEFLIDCGFIPGNKNKLQVACM  EEVA+KSDS+EI       + +W+GVKITEKLKL+DKIEL++ AKLEIV+IIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYED GKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNV    + KVDEIFS+K+       IQVEH EKQEIFEAPR                                         AVYP  EIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGIDKEVMLTSLTYMV 
Sbjct: 2022 GRQIIKVKFKNRYLEDDEFLIDCGFIPGNKNKLQVACMSKEEVAKKSDSQEI-------IGEWDGVKITEKLKLIDKIELEDKAKLEIVDIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNVNGIEEIKVDEIFSDKDENELNNKIQVEHFEKQEIFEAPR-----------------------------------------AVYPMIEIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGIDKEVMLTSLTYMVL 2358

HSP 3 Score: 269.24 bits (687), Expect = 7.045e-96
Identity = 160/274 (58.39%), Postives = 187/274 (68.25%), Query Frame = -3 
Query: 1498 FATAPTYLTEKLKEMKSVEHIQFELDKIYPTSAEIERKNMLGLPYSYAMMTI*NKGKQSRISILFNLRNVEALLDSGSSIYRIR*SVDN*R*IKYETNK*SHKINVTQCFKYN-----*KIKTEQILGNHELVHYVSVIRKSPIRCLIGMNVMENSNQLLLTY*LGD**NNKQTKEDIK*ERYF*IK--H*NYKQEVR*YYADVLEVSDD*CIFETNENLIQKYDVTMCDEVVRVKDQTVPVRIVNQSTKSIQMKPKTKLGKLTKFEIEKTQRF 2298
              TAP Y TEKLKEMK VEHIQFELD I PTSAEIERKNMLGLP SYAMMT+ NKGKQ RI+ILFN R VE+LLDSGSS       V +      E +K      V +   +N      K+K E I+G+HELVH+VSV+R SP+ CLIGM+VME    + L    G     +  ++  K    F  +      + EV  YYADVL+VSDD CIFE NE LI+KY++ MCDEVV+VKDQTVPVRIVNQ+TK IQMKPKTKLGKLTKFEIEKT + 
Sbjct: 3055 LPTAPKYPTEKLKEMKPVEHIQFELDNIEPTSAEIERKNMLGLPCSYAMMTLGNKGKQPRINILFNRRKVESLLDSGSS----NTIVSDDLLTMEEKSKMKPTSKVIRSMSHNVLSIIGKLKAELIIGDHELVHHVSVMRNSPVGCLIGMDVMEQLQGISLDLKTGRLMKGQTDEKRHKVREVFLNQTLELPARSEVI-YYADVLDVSDDECIFEPNEKLIEKYEIPMCDEVVKVKDQTVPVRIVNQNTKPIQMKPKTKLGKLTKFEIEKTPKL 3323

HSP 4 Score: 103.99 bits (258), Expect = 7.045e-96
Identity = 70/92 (76.09%), Postives = 75/92 (81.52%), Query Frame = -1 
Query: 2295 ARFFITHHQYREMTSVRAQLKTDQKSELSI*AASXXXXXXXXXXXXXXNGQRSFQKNNEQ*EKAIA-TILLEPMPKLSGIETLLKWYTPTIL 2567
            ARFFITHHQYREM SVRAQLKTDQKSE+S+ AASDHNNDNNDDI++NK  QR FQK   + EK      LLEPMPKLSGIETL KWYTPT L
Sbjct: 2964 ARFFITHHQYREMASVRAQLKTDQKSEVSLCAASDHNNDNNDDINDNKKEQRYFQKGIMKSEKKPELQFLLEPMPKLSGIETLSKWYTPTFL 3055
[Close]
BLAST of Holocarboxylase synthetase vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 1012.29 bits (2616), Expect = 0.000e+0
Identity = 507/624 (81.25%), Postives = 521/624 (83.49%), Query Frame = -1 
Query: 2754 LEIGAQVIAETDNLENSIEGGVRISHEYFIEWVKKNGGWKKLNNDLTVNESDEKKLPYKPLNGKDSPKLRKKMIFPWAIVMLFIFCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKVNGKIIKQSVVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNGEGKVNYEYLLTPDHFRKAT 4625
            LEIG   IAETDNLEN I+   RI +EYFIE       W K N                                                  GMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECI KRTTFVFFCGKWSHTSLIKMHAI R +EVMPENCLAA +TE LKINPEIKLKAK+NHRIRETVVVKGVI QDGTCE E Y          +VIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTM FKELS EQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMM HEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILR+RMAMAITNPNLVAPMLISENGVFARIFGEA YSYKCKA+TVKIMKYEKCTNELPVSINGKMRFMEPITRILLDE TK KILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASE+IEEA RNLLFPSKKERVISEMIDTILPNGE +    + +T   +R+  
Sbjct:  961 LEIGPHAIAETDNLENLIDVAARILNEYFIE-------WVKKNG-------------------------------------------------GMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIVKRTTFVFFCGKWSHTSLIKMHAISRQLEVMPENCLAAFQTEELKINPEIKLKAKINHRIRETVVVKGVITQDGTCEGEPY----------MVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMTFKELSGEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMGHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRSRMAMAITNPNLVAPMLISENGVFARIFGEAFYSYKCKAVTVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEHTKQKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASEDIEEAQRNLLFPSKKERVISEMIDTILPNGEAR----FFITHHQYRETA 1514

HSP 2 Score: 624.394 bits (1609), Expect = 0.000e+0
Identity = 332/385 (86.23%), Postives = 348/385 (90.39%), Query Frame = -3 
Query: 4624 GKQ--SLK*ENMNLEDDEFLIDCGFIPGNKNKLQVACMISEEVAEKSDSKEITRDLRDALSDWNGVKITEKLKLLDKIELQENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDCGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKXXXXXXXXXXXDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNV----KTKVDEIFSNKE-------IQVEHLEKQEIFEAPRGEINSEIIVSKPRTLYEIKQSLVKIEQEVDETKIILMGLIKAVYPTFEIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGIDKEVMLTSLTYMVF 5739
            G+Q   +K +N  LEDDEFLIDCGFIPGNKNKLQVACMI EEVAEKSDS++I  DLR+AL++W+GVKITEKLKL+DKIEL ENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYED GKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLD DGRAGYLVNCVVAIKEQKVD VDNSENSNTDIEEDN+    +TKVDEIFS+KE       IQ EH EKQEIFEAPRGEINSEIIV KPRTLYEIK+SLV+IEQEVDETKIILM LIKAVYP FEIKMDVEYELGN+VTEMKNRL  V QRFGENDNFCDKKLI NL KNG DKEVMLTSLTY V 
Sbjct:  577 GRQIIKVKFKNRYLEDDEFLIDCGFIPGNKNKLQVACMIREEVAEKSDSQKIIGDLREALNEWDGVKITEKLKLIDKIELDENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDGDGRAGYLVNCVVAIKEQKVDPVDNSENSNTDIEEDNINGIEETKVDEIFSDKEENELNNKIQAEHFEKQEIFEAPRGEINSEIIVRKPRTLYEIKRSLVRIEQEVDETKIILMSLIKAVYPMFEIKMDVEYELGNMVTEMKNRLLDVGQRFGENDNFCDKKLIGNLTKNGNDKEVMLTSLTYTVL 961

HSP 3 Score: 272.322 bits (695), Expect = 2.690e-95
Identity = 161/274 (58.76%), Postives = 190/274 (69.34%), Query Frame = -3 
Query: 1498 FATAPTYLTEKLKEMKSVEHIQFELDKIYPTSAEIERKNMLGLPYSYAMMTI*NKGKQSRISILFNLRNVEALLDSGSSIYRIR*SVDN*R*IKYETNK*SHKINVTQCFKYN-----*KIKTEQILGNHELVHYVSVIRKSPIRCLIGMNVMENSNQLLLTY*LGD**NNKQTKEDIK*ERYF*IK--H*NYKQEVR*YYADVLEVSDD*CIFETNENLIQKYDVTMCDEVVRVKDQTVPVRIVNQSTKSIQMKPKTKLGKLTKFEIEKTQRF 2298
              TAPTY TEKLKEMK VEHIQFELD I PTSAEIERKNMLGLP SYAMMT+ NKGKQ RI+ILFN R VEALLDSGSS   +    D+   I+ E +K      V +   +N      K+K E I+G+HEL HYVSV+R SP+ CLIGM+VME   ++ L    G     +  ++  K    F  +      + EV  YYADVL+VSDD CIFE NE LI+KY++ MCDEVV+VKDQTVPVRIVNQ+TK IQMKPKT+LGKLTKFEIEK  + 
Sbjct: 1592 LPTAPTYSTEKLKEMKPVEHIQFELDNIEPTSAEIERKNMLGLPCSYAMMTLGNKGKQPRINILFNGRKVEALLDSGSSNTIVS---DDLLTIE-EKSKMKPTSKVIRSMSHNVLSIIEKLKAELIIGDHELEHYVSVMRNSPVGCLIGMDVMEQLQEISLHLKTGRLMKGQTDEKGHKVREVFLNQTLELPARSEVI-YYADVLDVSDDECIFEPNEKLIEKYEIPMCDEVVKVKDQTVPVRIVNQNTKPIQMKPKTRLGKLTKFEIEKIPKL 1860

HSP 4 Score: 98.9821 bits (245), Expect = 2.690e-95
Identity = 67/92 (72.83%), Postives = 73/92 (79.35%), Query Frame = -1 
Query: 2295 ARFFITHHQYREMTSVRAQLKTDQKSELSI*AASXXXXXXXXXXXXXXNGQRSFQKNNEQ*EKAIA-TILLEPMPKLSGIETLLKWYTPTIL 2567
            ARFFITHHQYRE  SVRAQLKTDQKSE+S+ A SD+NNDNNDDI++NK  QR FQK   + EK      LLEPMPKLSGIETL KWYTPT L
Sbjct: 1501 ARFFITHHQYRETASVRAQLKTDQKSEVSLCAGSDNNNDNNDDINDNKKEQRYFQKGIIKSEKKPELQFLLEPMPKLSGIETLSKWYTPTFL 1592
[Close]
BLAST of Holocarboxylase synthetase vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 1010.75 bits (2612), Expect = 0.000e+0
Identity = 507/624 (81.25%), Postives = 521/624 (83.49%), Query Frame = -1 
Query: 2754 LEIGAQVIAETDNLENSIEGGVRISHEYFIEWVKKNGGWKKLNNDLTVNESDEKKLPYKPLNGKDSPKLRKKMIFPWAIVMLFIFCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKVNGKIIKQSVVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNGEGKVNYEYLLTPDHFRKAT 4625
            LEIG   IAETDNLEN I+   RI +EYFIE       W K N                                                  GMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECI KRTTFVFFCGKWSHTSLIKMHAI R +EVMPENCLAA +TE LKINPEIKLKAK+NHRIRETVVVKGVI QDGTCE E Y          +VIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTM FKELS EQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMM HEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILR+RMAMAITNPNLVAPMLISENGVFARIFGEA YSYKCKA+TVKIMKYEKCTNELPVSINGKMRFMEPITRILLDE TK KILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASE+IEEA RNLLFPSKKERVISEMIDTILPNGE +    + +T   +R+  
Sbjct:  961 LEIGPHAIAETDNLENLIDVAARILNEYFIE-------WVKKNG-------------------------------------------------GMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIVKRTTFVFFCGKWSHTSLIKMHAISRQLEVMPENCLAAFQTEELKINPEIKLKAKINHRIRETVVVKGVITQDGTCEGEPY----------MVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMTFKELSGEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMGHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRSRMAMAITNPNLVAPMLISENGVFARIFGEAFYSYKCKAVTVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEHTKQKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASEDIEEAQRNLLFPSKKERVISEMIDTILPNGEAR----FFITHHQYRETA 1514

HSP 2 Score: 624.78 bits (1610), Expect = 0.000e+0
Identity = 332/385 (86.23%), Postives = 348/385 (90.39%), Query Frame = -3 
Query: 4624 GKQ--SLK*ENMNLEDDEFLIDCGFIPGNKNKLQVACMISEEVAEKSDSKEITRDLRDALSDWNGVKITEKLKLLDKIELQENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDCGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKXXXXXXXXXXXDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNV----KTKVDEIFSNKE-------IQVEHLEKQEIFEAPRGEINSEIIVSKPRTLYEIKQSLVKIEQEVDETKIILMGLIKAVYPTFEIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGIDKEVMLTSLTYMVF 5739
            G+Q   +K +N  LEDDEFLIDCGFIPGNKNKLQVACMI EEVAEKSDS++I  DLR+AL++W+GVKITEKLKL+DKIEL ENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYED GKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLD DGRAGYLVNCVVAIKEQKVD VDNSENSNTDIEEDN+    +TKVDEIFS+KE       IQ EH EKQEIFEAPRGEINSEIIV KPRTLYEIK+SLV+IEQEVDETKIILM LIKAVYP FEIKMDVEYELGN+VTEMKNRL  V QRFGENDNFCDKKLI NL KNG DKEVMLTSLTY V 
Sbjct:  577 GRQIIKVKFKNRYLEDDEFLIDCGFIPGNKNKLQVACMIREEVAEKSDSQKIIGDLREALNEWDGVKITEKLKLIDKIELDENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDGDGRAGYLVNCVVAIKEQKVDPVDNSENSNTDIEEDNINGIEETKVDEIFSDKEENELNNKIQAEHFEKQEIFEAPRGEINSEIIVRKPRTLYEIKRSLVRIEQEVDETKIILMSLIKAVYPMFEIKMDVEYELGNMVTEMKNRLLDVGQRFGENDNFCDKKLIGNLTKNGNDKEVMLTSLTYTVL 961

HSP 3 Score: 272.707 bits (696), Expect = 1.851e-95
Identity = 161/274 (58.76%), Postives = 190/274 (69.34%), Query Frame = -3 
Query: 1498 FATAPTYLTEKLKEMKSVEHIQFELDKIYPTSAEIERKNMLGLPYSYAMMTI*NKGKQSRISILFNLRNVEALLDSGSSIYRIR*SVDN*R*IKYETNK*SHKINVTQCFKYN-----*KIKTEQILGNHELVHYVSVIRKSPIRCLIGMNVMENSNQLLLTY*LGD**NNKQTKEDIK*ERYF*IK--H*NYKQEVR*YYADVLEVSDD*CIFETNENLIQKYDVTMCDEVVRVKDQTVPVRIVNQSTKSIQMKPKTKLGKLTKFEIEKTQRF 2298
              TAPTY TEKLKEMK VEHIQFELD I PTSAEIERKNMLGLP SYAMMT+ NKGKQ RI+ILFN R VEALLDSGSS   +    D+   I+ E +K      V +   +N      K+K E I+G+HEL HYVSV+R SP+ CLIGM+VME   ++ L    G     +  ++  K    F  +      + EV  YYADVL+VSDD CIFE NE LI+KY++ MCDEVV+VKDQTVPVRIVNQ+TK IQMKPKT+LGKLTKFEIEK  + 
Sbjct: 1592 LPTAPTYSTEKLKEMKPVEHIQFELDNIEPTSAEIERKNMLGLPCSYAMMTLGNKGKQPRINILFNGRKVEALLDSGSSNTIVS---DDLLTIE-EKSKMKPTSKVIRSMSHNVLSIIEKLKAELIIGDHELEHYVSVMRNSPVGCLIGMDVMEQLQEISLHLKTGRLMKGQTDEKGHKVREVFLNQTLELPARSEVI-YYADVLDVSDDECIFEPNEKLIEKYEIPMCDEVVKVKDQTVPVRIVNQNTKPIQMKPKTRLGKLTKFEIEKIPKL 1860

HSP 4 Score: 98.9821 bits (245), Expect = 1.851e-95
Identity = 67/92 (72.83%), Postives = 73/92 (79.35%), Query Frame = -1 
Query: 2295 ARFFITHHQYREMTSVRAQLKTDQKSELSI*AASXXXXXXXXXXXXXXNGQRSFQKNNEQ*EKAIA-TILLEPMPKLSGIETLLKWYTPTIL 2567
            ARFFITHHQYRE  SVRAQLKTDQKSE+S+ A SD+NNDNNDDI++NK  QR FQK   + EK      LLEPMPKLSGIETL KWYTPT L
Sbjct: 1501 ARFFITHHQYRETASVRAQLKTDQKSEVSLCAGSDNNNDNNDDINDNKKEQRYFQKGIIKSEKKPELQFLLEPMPKLSGIETLSKWYTPTFL 1592
[Close]
BLAST of Holocarboxylase synthetase vs. Planmine SMEST
Match: SMESG000057446.1 (SMESG000057446.1)

HSP 1 Score: 1009.98 bits (2610), Expect = 0.000e+0
Identity = 507/624 (81.25%), Postives = 521/624 (83.49%), Query Frame = -1 
Query: 2754 LEIGAQVIAETDNLENSIEGGVRISHEYFIEWVKKNGGWKKLNNDLTVNESDEKKLPYKPLNGKDSPKLRKKMIFPWAIVMLFIFCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMHAIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQDGTCE*ELYKVNGKIIKQSVVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNGEGKVNYEYLLTPDHFRKAT 4625
            LEIG   IAETDNLEN I+   RI +EYFIE       W K N                                                  GMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECI KRTTFVFFCGKWSHTSLIKMHAI R +EVMPENCLAA +TE LKINPEIKLKAK+NHRIRETVVVKGVI QDGTCE E Y          +VIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTM FKELS EQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMM HEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILR+RMAMAITNPNLVAPMLISENGVFARIFGEA YSYKCKA+TVKIMKYEKCTNELPVSINGKMRFMEPITRILLDE TK KILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASE+IEEA RNLLFPSKKERVISEMIDTILPNGE +    + +T   +R+  
Sbjct:  961 LEIGPHAIAETDNLENLIDVAARILNEYFIE-------WVKKNG-------------------------------------------------GMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECIVKRTTFVFFCGKWSHTSLIKMHAISRQLEVMPENCLAAFQTEELKINPEIKLKAKINHRIRETVVVKGVITQDGTCEGEPY----------MVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMTFKELSGEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMGHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRSRMAMAITNPNLVAPMLISENGVFARIFGEAFYSYKCKAVTVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEHTKQKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASEDIEEAQRNLLFPSKKERVISEMIDTILPNGEAR----FFITHHQYRETA 1514

HSP 2 Score: 625.165 bits (1611), Expect = 0.000e+0
Identity = 332/385 (86.23%), Postives = 348/385 (90.39%), Query Frame = -3 
Query: 4624 GKQ--SLK*ENMNLEDDEFLIDCGFIPGNKNKLQVACMISEEVAEKSDSKEITRDLRDALSDWNGVKITEKLKLLDKIELQENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDCGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKXXXXXXXXXXXDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNV----KTKVDEIFSNKE-------IQVEHLEKQEIFEAPRGEINSEIIVSKPRTLYEIKQSLVKIEQEVDETKIILMGLIKAVYPTFEIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGIDKEVMLTSLTYMVF 5739
            G+Q   +K +N  LEDDEFLIDCGFIPGNKNKLQVACMI EEVAEKSDS++I  DLR+AL++W+GVKITEKLKL+DKIEL ENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYED GKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLD DGRAGYLVNCVVAIKEQKVD VDNSENSNTDIEEDN+    +TKVDEIFS+KE       IQ EH EKQEIFEAPRGEINSEIIV KPRTLYEIK+SLV+IEQEVDETKIILM LIKAVYP FEIKMDVEYELGN+VTEMKNRL  V QRFGENDNFCDKKLI NL KNG DKEVMLTSLTY V 
Sbjct:  577 GRQIIKVKFKNRYLEDDEFLIDCGFIPGNKNKLQVACMIREEVAEKSDSQKIIGDLREALNEWDGVKITEKLKLIDKIELDENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDWGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDGDGRAGYLVNCVVAIKEQKVDPVDNSENSNTDIEEDNINGIEETKVDEIFSDKEENELNNKIQAEHFEKQEIFEAPRGEINSEIIVRKPRTLYEIKRSLVRIEQEVDETKIILMSLIKAVYPMFEIKMDVEYELGNMVTEMKNRLLDVGQRFGENDNFCDKKLIGNLTKNGNDKEVMLTSLTYTVL 961

HSP 3 Score: 272.707 bits (696), Expect = 1.851e-95
Identity = 161/274 (58.76%), Postives = 190/274 (69.34%), Query Frame = -3 
Query: 1498 FATAPTYLTEKLKEMKSVEHIQFELDKIYPTSAEIERKNMLGLPYSYAMMTI*NKGKQSRISILFNLRNVEALLDSGSSIYRIR*SVDN*R*IKYETNK*SHKINVTQCFKYN-----*KIKTEQILGNHELVHYVSVIRKSPIRCLIGMNVMENSNQLLLTY*LGD**NNKQTKEDIK*ERYF*IK--H*NYKQEVR*YYADVLEVSDD*CIFETNENLIQKYDVTMCDEVVRVKDQTVPVRIVNQSTKSIQMKPKTKLGKLTKFEIEKTQRF 2298
              TAPTY TEKLKEMK VEHIQFELD I PTSAEIERKNMLGLP SYAMMT+ NKGKQ RI+ILFN R VEALLDSGSS   +    D+   I+ E +K      V +   +N      K+K E I+G+HEL HYVSV+R SP+ CLIGM+VME   ++ L    G     +  ++  K    F  +      + EV  YYADVL+VSDD CIFE NE LI+KY++ MCDEVV+VKDQTVPVRIVNQ+TK IQMKPKT+LGKLTKFEIEK  + 
Sbjct: 1592 LPTAPTYSTEKLKEMKPVEHIQFELDNIEPTSAEIERKNMLGLPCSYAMMTLGNKGKQPRINILFNGRKVEALLDSGSSNTIVS---DDLLTIE-EKSKMKPTSKVIRSMSHNVLSIIEKLKAELIIGDHELEHYVSVMRNSPVGCLIGMDVMEQLQEISLHLKTGRLMKGQTDEKGHKVREVFLNQTLELPARSEVI-YYADVLDVSDDECIFEPNEKLIEKYEIPMCDEVVKVKDQTVPVRIVNQNTKPIQMKPKTRLGKLTKFEIEKIPKL 1860

HSP 4 Score: 98.9821 bits (245), Expect = 1.851e-95
Identity = 67/92 (72.83%), Postives = 73/92 (79.35%), Query Frame = -1 
Query: 2295 ARFFITHHQYREMTSVRAQLKTDQKSELSI*AASXXXXXXXXXXXXXXNGQRSFQKNNEQ*EKAIA-TILLEPMPKLSGIETLLKWYTPTIL 2567
            ARFFITHHQYRE  SVRAQLKTDQKSE+S+ A SD+NNDNNDDI++NK  QR FQK   + EK      LLEPMPKLSGIETL KWYTPT L
Sbjct: 1501 ARFFITHHQYRETASVRAQLKTDQKSEVSLCAGSDNNNDNNDDINDNKKEQRYFQKGIIKSEKKPELQFLLEPMPKLSGIETLSKWYTPTFL 1592
[Close]
BLAST of Holocarboxylase synthetase vs. Planmine SMEST
Match: SMESG000056661.1 (SMESG000056661.1)

HSP 1 Score: 691.419 bits (1783), Expect = 0.000e+0
Identity = 377/537 (70.20%), Postives = 396/537 (73.74%), Query Frame = -1 
Query: 2295 VVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNINYKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNYRGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKCKAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRLLAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASENIEEA*RNLLFPSKKERVISEMIDTILPNGEGKVNYEYLLTPDHFRKATYKTMKKIWGKFMFLGQACAGLIGFYVVIITIKVIMSQILSAHRIYVISGWSWKIIIGCFPFLARFFITHHQYREMTSVRAQLKTDQKSELSI*AASXXXXXXXXXXXXXXNGQRSFQKNNEQ*EKAIA-TILLEPMPKLSGIETLLKWYTPTIL 3902
            +VIEEYLTELREFEGVFDDETGKLQTHPFCNMRQ N QTGESILVFDVKTNVCNLAHLKTM FKELS +QFRESPDNFNINYKSNGTIRST VWTRKTTPVLMSTLPTDSMRF KKEATNKCGQMVYATNY GVYVSKMMV EAKTKIHTNDIKLTHYFNNKIDY YHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITN NLVAPMLISENGVFARIFG+ALY+YKCKA+TVKIMKYE CTNEL ++                                 +  L+QDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASE+IEEA RNLLFPSKKERVISEM+DTILPNGE    Y                    W   M +G  C  L+                                         RFFITHHQYREM SVRAQLKTDQKSE+S+ AASDHNNDNNDDI +NK  Q+ FQK   + EK      LLEPMPKLSGI+TL KW  PT L
Sbjct:  851 LVIEEYLTELREFEGVFDDETGKLQTHPFCNMRQRNSQTGESILVFDVKTNVCNLAHLKTMTFKELSGQQFRESPDNFNINYKSNGTIRSTSVWTRKTTPVLMSTLPTDSMRFTKKEATNKCGQMVYATNYSGVYVSKMMVQEAKTKIHTNDIKLTHYFNNKIDYYYHFSQNIMENIYREMILNDCKLNKEILRNRMAMAITNSNLVAPMLISENGVFARIFGDALYTYKCKAVTVKIMKYEMCTNELQIT-------------------------------CSVVSLSQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFYASEDIEEAQRNLLFPSKKERVISEMVDTILPNGEAHRIYAI----------------SGWSWEMIIG--CFPLLA----------------------------------------RFFITHHQYREMASVRAQLKTDQKSEVSLCAASDHNNDNNDDIGHNKKEQKYFQKGIMKSEKKPELQFLLEPMPKLSGIQTLSKWSKPTFL 1298

HSP 2 Score: 485.337 bits (1248), Expect = 0.000e+0
Identity = 265/385 (68.83%), Postives = 280/385 (72.73%), Query Frame = -3 
Query: 4624 GKQ--SLK*ENMNLEDDEFLIDCGFIPGNKNKLQVACMISEEVAEKSDSKEITRDLRDALSDWNGVKITEKLKLLDKIELQENAKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETRMELKPFIIAIDYEDCGKTEYIEEHWCTKVQKLRKRAANQIGKDGIIVLMFKXXXXXXXXXXXDCGFKPQELDENGYLDADGRAGYLVNCVVAIKEQKVDSVDNSENSNTDIEEDNV----KTKVDEIFSNKE-------IQVEHLEKQEIFEAPRGEINSEIIVSKPRTLYEIKQSLVKIEQEVDETKIILMGLIKAVYPTFEIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGIDKEVMLTSLTYMVF 5739
            G+Q   +K +N  LEDDEFLIDCGFIPGNKNKLQVACMI EEV EKSDS+EITRDLRDALS+W+GVKITEKL+LLDKIEL+E  KLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRET MELKPFIIAIDYED GK EYIEEHWCTKVQK                                  F PQELDENGYLDADGR GYLVNCVVA+KEQK+D V N ENSNTDIEEDNV    + K+DEIFS KE         V+H EKQEIFE PRGEINSEIIV KP TLYEIKQSLV+IEQEVDETKIILMGLIKA YP FEIKMDVE             LP VVQRF                 NGIDKEVMLTSLTY+V 
Sbjct:  382 GRQIIKVKFKNRYLEDDEFLIDCGFIPGNKNKLQVACMIREEVMEKSDSQEITRDLRDALSEWDGVKITEKLELLDKIELEEKTKLEIVNIIRPYLYDKSRYGEKLKCLMRRFGRELRETGMELKPFIIAIDYEDWGKAEYIEEHWCTKVQK----------------------------------FNPQELDENGYLDADGRVGYLVNCVVALKEQKIDLVYNLENSNTDIEEDNVNGIEREKIDEIFSEKEENDLNNKSHVDHFEKQEIFETPRGEINSEIIVRKPITLYEIKQSLVRIEQEVDETKIILMGLIKATYPMFEIKMDVE-------------LPNVVQRFD----------------NGIDKEVMLTSLTYIVL 703

HSP 3 Score: 250.366 bits (638), Expect = 0.000e+0
Identity = 121/150 (80.67%), Postives = 130/150 (86.67%), Query Frame = -1 
Query: 4176 LEIGAQVIAETDNLENSIEGGVRISHEYFIEWVKKNGGWKKLNNDLTVNESDEKKLPYKPLNGKDSPKLRKKMIFPWAIVMLFIFCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPDAYPKKLKVENDVKYYVYQESDLRRAKVRECI 4625
            LEIGA  IAETD LE  I+   RI +EYFIEWVKK GGWK+LNNDLTVNESDE+KL  KPLNGKDSP LRKKMIFPWAIVMLFI C+ITTQATGMTAYDC QTEM K+YS V+MAEC DAYPKKLKVENDVKYYVYQESDLR+AKV E +
Sbjct:  703 LEIGAHAIAETDKLEYLIDVAARILNEYFIEWVKKKGGWKELNNDLTVNESDERKLSCKPLNGKDSPGLRKKMIFPWAIVMLFILCSITTQATGMTAYDCFQTEMVKMYSFVNMAECLDAYPKKLKVENDVKYYVYQESDLRQAKVIELV 852

HSP 4 Score: 248.825 bits (634), Expect = 0.000e+0
Identity = 152/270 (56.30%), Postives = 181/270 (67.04%), Query Frame = -3 
Query: 1510 FATAPTYLTEKLKEMKSVEHIQFELDKIYPTSAEIERKNMLGLPYSYAMMTI*NKGKQSRISILFNLRNVEALLDSGSSIYRIR*SVDN*R*IKYETNK*SHKINVTQCFKYN-----*KIKTEQILGNHELVHYVSVIRKSPIRCLIGMNVMENSNQLLLTY*LGD**NNKQTKEDIK*ERYF*IKH*NYKQEVR*YYAD-VLEV-SDD*CIFETNENLIQKYDVTMCDEVVRVKDQTVPVRIVNQSTKSIQMKPKTKLGKLTKFEIEK 2298
             +TAPTYLT+KLKEMK VEHIQFELD I PTSAEIERKNMLGLP SYAMMT+ NKGKQ RI ILF  R VEALLDSGSS   +    D+   IK E +K      V +   +N      K K E I+G+HEL+HYVSV+R SP+ CLIGM+VME    + L               D+K  R    +      +VR  + +  LE+ +   CIFETNE LI+KY++ MCDE+V+VKDQT+P RIVNQ+TKSIQMKPKTKLGKL KFEIEK
Sbjct: 1298 LSTAPTYLTKKLKEMKPVEHIQFELDNIEPTSAEIERKNMLGLPCSYAMMTLGNKGKQPRIYILFTGRKVEALLDSGSSNTIVS---DDLLTIK-EKSKIKSTSKVIRSMLHNVLSIIGKFKAEIIIGDHELMHYVSVMRNSPVGCLIGMDVMEQLQGISL---------------DLKTGRLVKRQTDEKGHKVREVFLNQTLELPARSECIFETNEKLIEKYEIPMCDEMVKVKDQTMPARIVNQNTKSIQMKPKTKLGKLMKFEIEK 1548

HSP 5 Score: 65.855 bits (159), Expect = 3.834e-10
Identity = 34/45 (75.56%), Postives = 38/45 (84.44%), Query Frame = -2 
Query: 1718 KL*SITLDLLTGRLMK*QTDERGHKVREVFLNQTLELQARSEVIL 1852
            +L  I+LDL TGRL+K QTDE+GHKVREVFLNQTLEL ARSE I 
Sbjct: 1448 QLQGISLDLKTGRLVKRQTDEKGHKVREVFLNQTLELPARSECIF 1492
The following BLAST results are available for this feature:
BLAST of Holocarboxylase synthetase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
HLCS1.292e-1943.43holocarboxylase synthetase [Source:HGNC Symbol;Acc... [more]
HLCS1.292e-1943.43holocarboxylase synthetase [Source:HGNC Symbol;Acc... [more]
HLCS1.292e-1943.43holocarboxylase synthetase [Source:HGNC Symbol;Acc... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
bpl-13.532e-1230.09Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Ac... [more]
bpl-13.532e-1230.09Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Ac... [more]
bpl-14.260e-1230.09Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Ac... [more]
bpl-18.411e-1232.08Biotin Protein Ligase [Source:UniProtKB/TrEMBL;Ac... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Hcs4.842e-1333.98gene:FBgn0037332 transcript:FBtr0078683[more]
Hcs4.944e-1333.98gene:FBgn0037332 transcript:FBtr0336769[more]
Hcs5.172e-1333.98gene:FBgn0037332 transcript:FBtr0345254[more]
Hcs5.194e-1333.98gene:FBgn0037332 transcript:FBtr0345253[more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Holocarboxylase synthetase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
en21.141e-1740.91engrailed homeobox 2 [Source:Xenbase;Acc:XB-GENE-8... [more]
en21.326e-1740.91engrailed homeobox 2 [Source:Xenbase;Acc:XB-GENE-8... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Hlcs1.215e-1936.36holocarboxylase synthetase (biotin- [propriony-Coe... [more]
Hlcs1.235e-1836.36holocarboxylase synthetase (biotin- [propriony-Coe... [more]
Hlcs1.235e-1836.36holocarboxylase synthetase (biotin- [propriony-Coe... [more]
Hlcs2.486e-1836.36holocarboxylase synthetase (biotin- [propriony-Coe... [more]
Hlcs2.732e-1439.77holocarboxylase synthetase (biotin- [propriony-Coe... [more]
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BLAST of Holocarboxylase synthetase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|P50747|BPL1_HUMAN6.207e-1943.43Biotin--protein ligase OS=Homo sapiens OX=9606 GN=... [more]
sp|Q920N2|BPL1_MOUSE8.636e-1836.36Biotin--protein ligase OS=Mus musculus OX=10090 GN... [more]
sp|F4I4W2|HCS2_ARATH1.853e-730.63Biotin--protein ligase 2 OS=Arabidopsis thaliana O... [more]
sp|Q9SL92|HCS1_ARATH8.422e-728.46Biotin--protein ligase 1, chloroplastic OS=Arabido... [more]
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BLAST of Holocarboxylase synthetase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A355ABF24.814e-13337.28Uncharacterized protein OS=Flavobacteriaceae bacte... [more]
A0A151IFZ03.389e-3923.09Uncharacterized protein OS=Cyphomyrmex costatus OX... [more]
A0A0L7QJ863.074e-3824.03Uncharacterized protein OS=Habropoda laboriosa OX=... [more]
A0A0B5KJV81.353e-3724.11Glycoprotein OS=Tacheng Tick Virus 4 OX=1608086 GN... [more]
A0A151J7N22.718e-3724.10Uncharacterized protein OS=Trachymyrmex cornetzi O... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hlcs7.205e-1835.85holocarboxylase synthetase [Source:NCBI gene;Acc:1... [more]
hlcs7.786e-1835.85holocarboxylase synthetase [Source:NCBI gene;Acc:1... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
HLCS1.726e-1730.00holocarboxylase synthetase [Source:HGNC Symbol;Acc... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
BPL13.275e-724.60Biotin:apoprotein ligase; covalently modifies prot... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO410561.747e-1037.29Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Holocarboxylase synthetase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hlcs4.057e-1636.52holocarboxylase synthetase [Source:NCBI gene;Acc:1... [more]
hlcs4.544e-1636.52holocarboxylase synthetase [Source:NCBI gene;Acc:1... [more]
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BLAST of Holocarboxylase synthetase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000080881.11.328e-15591.64SMESG000080881.1[more]
SMESG000057446.12.690e-9581.25SMESG000057446.1[more]
SMESG000057446.11.851e-9581.25SMESG000057446.1[more]
SMESG000057446.11.851e-9581.25SMESG000057446.1[more]
SMESG000056661.13.834e-1070.20SMESG000056661.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004174 ID=SMED30004174|Name=Holocarboxylase synthetase|organism=Schmidtea mediterranea sexual|type=transcript|length=5741bp
ATGATTTCAAAATTTTTTATAAAAAATAAACTTTTTAATATTTTTGACAT
TTTTGTTCGCCGTAATCATACATTAAAAATGGAATTAGTAGACATAATTA
CACATTCTGTAAACTATTCTGAGAAATTTTTCATGCTATTGAAAACCATT
TTTTCTCCAAACAAATTTTTATTATATGATGTAAATAAAAATGGATTATT
GGAAAGAAGTTGGTACAGATCTTTCAGCATATTAGTTATTTATACTCCTG
ATATTAATGTATATGATAAAGAATTAATGAAAATTAATCCATTTGTGATG
GGAAAAAAATTATATTAATTTGCGAAAACTTTGAATTTGGAATCTTTAAA
GAGGCTAAATATGGTTCATATTATATTTTAGAAAATAATGTTTACGAAAT
TCAGAAAGATAATAAAACGCTTTTGTTACTTTTTGAAAATTTAGATGCAT
TTTATAAAAATCATGGATTAGTATCTAAAATATGGGATTTAACGAAATAT
TCAATTAATCAAATTCAATGTCATCACGGTGATTTATATGTTTTTTCTGA
AAATCACGAAAGCATTTCTTCATATTTAGAGTCTATTAAATTAAGTGATC
CTTTATTGAATAAATTTCAGTATAACTCATTAGAATTTAATGTAAATCTG
TCGGATAATCAAATTTTCGATTCTAATAAACCGTTTAAATTATTAACAAA
TATCAATGATAAAAATCCAGATTTCCAAGAAATTGTATCGTTTAAATCAA
ATGATTATTTTTCATCGCTCTACACAAAATACTTGGGGAAAATTGTCTTA
TTTTCTGAGGTTTTGTCATCGAGTTTTGATTTAATGGAAAGGATTGTCCA
ATTAGGCAATTTCCAGCCAATTGTTATTATATCTTATGTTCAAACTGCTG
GTAAAGGAAGGGGTTCTAATAAATGGATTAGCCCCTTAGGTTGTGCCATG
TATACATGTTTAATTCCGATACAAATTCGTTCCAGGCTTGGGAATTCAAT
GAGTCTCATTCAGCATATCATGGCTGTGAGTATTGTCGAGGCTATAAATT
TTCTCTGTTGGATTGATGTGAGTGTAAATTCAGCTTTAATTTCAGATAAG
AATCTTATTTTTAAGAATCCATAAGAAATTTATTAAAAATCAATCTATTT
TTTAAACAAATTTTCCATTTTTTATTTTTTTAAAATTTATATAAATTTTT
AATTTAAAATACTTTGATATTGAATAATGTTCCATACTCAATCATCTTTT
TTACCGAAAATAATACTGTTGGGGTTAGATGATTTTCAATAAAAAAAATT
ATAATAATAAAAAATGGGGGAAAATAAAATAATTAATTAAAAATAATCAA
AAAATTAAAATCAATATTTTCCGGGGAAATGGGTGTGTGCCGATGCCAAT
TGACGAGACGAGTACGAAAATAATAATATAAAATTAATTTGGGACCCTTA
AAAAAGTTTTAAGAAAATACAAATTTATTAATTTATTAAATAAGGAGAAA
TCTTTGAGTTTTCTCTATTTCAAATTTTGTTAGTTTTCCCAGTTTTGTTT
TTGGTTTCATTTGAATGGATTTTGTGCTTTGATTAACTATTCTCACTGGC
ACTGTTTGGTCTTTGACTCTTACCACTTCATCACACATTGTTACATCATA
TTTTTGTATAAGATTCTCATTTGTTTCAAATATGCACTAGTCATCTGAAA
CCTCTAACACATCCGCGTAATATTACCTCACTTCTTGCTTGTAGTTCTAG
TGTTTGATTTAGAAATACCTCTCTTACTTTATGTCCTCTTTCGTCTGTTT
GTTATTTCATTAATCTCCCAGTTAATAGGTCAAGAGTAATTGATTAGAGT
TTTCCATCACATTCATGCCAATTAAACACCTGATCGGGCTTTTCCTTATT
ACTGACACATAATGTACTAATTCGTGATTACCAAGGATTTGTTCAGTTTT
GATTTTTCAATTATATTTAAAACATTGAGTCACATTGATCTTATGACTTT
ACTTGTTGGTTTCATATTTGATTTATCTTCAATTGTCAACAGATCATCTG
ATTCTATAAATACTGGAACCTGAATCTAATAAAGCTTCCACATTTCTTAG
ATTGAATAGAATGCTTATACGAGATTGTTTGCCTTTATTTCATATCGTCA
TCATTGCATAACTGTATGGTAATCCGAGCATATTTTTCCGTTCAATTTCA
GCGCTTGTTGGATAAATTTTATCTAATTCAAATTGAATATGTTCTACTGA
TTTCATTTCTTTCAATTTTTCAGTTAGATACGTTGGTGCTGTTGCAAAAT
TGTTGGTGTGTACCATTTTAAAAGCGTTTCAATTCCGCTAAGTTTCGGCA
TAGGTTCCAATAAAATTGTAGCTATGGCTTTTTCTCATTGTTCATTATTT
TTTTGAAAAGATCTTTGTCCATTTTTATTATTATCGATGTCATCATTATT
ATCGTTATTGTGATCACTTGCTGCTCATATAGACAATTCGCTTTTTTGAT
CGGTTTTCAGTTGTGCTCTCACACTTGTCATTTCTCTATATTGATGATGA
GTAATGAAAAATCTCGCTAAAAATGGAAAACAACCAATAATTATTTTCCA
TGACCATCCGGATATTACGTATATTCTATGAGCCGATAATATTTGTGACA
TTATTACCTTTATTGTTATGATTACAACATAGAATCCAATTAAGCCTGCG
CATGCTTGTCCTAAAAACATGAATTTCCCCCAGATCTTTTTCATCGTTTT
ATATGTTGCCTTTCTAAAGTGATCTGGTGTTAGAAGATATTCATAATTGA
CTTTACCTTCCCCGTTAGGCAAAATTGTATCAATCATTTCTGAGATTACA
CGTTCCTTTTTTGACGGAAATAACAAATTTCTTTAAGCTTCTTCAATATT
CTCACTTGCGTAGAAACCCATTTGTGCCATCTTATTTAAGGATGTAAAAT
TTACTAGGTATTCTAGATTTGTTAATTCTAATTTATCCGGAGTTCTCACT
CCTCGGTAATCTGGTAATGCTATCCATGAATCCTGTGCTAAGCGATACAA
CGGAGCAAGTAATCTATTACATTGAAGAATTTTTGGCTTAGTTTGCTCGT
CCAGTAATATTCTTGTTATGGGTTCCATAAATCGCATTTTTCCGTTTATA
CTCACCGGCAATTCATTTGTACATTTTTCGTATTTCATAATTTTTACTGT
AATTGCTTTGCATTTGTAGCTATATAGGGCCTCTCCGAAGATTCTGGCAA
ATACACCGTTTTCTGATATTAGCATAGGTGCTACTAAGTTTGGATTTGTA
ATGGCCATTGCCATCCTGTTTCTAAGTATCTCTTTATTCAATTTGCAATC
ATTTAAAATCATTTCCCGATATATATTCTCCATTATGTTTTGACTAAAAT
GGTACAAATAATCAATTTTATTGTTAAAATAGTGAGTGAGTTTAATATCA
TTTGTGTGTATTTTAGTTTTCGCCTCATGCACCATCATTTTTGATACGTA
AACACCTCTGTAATTTGTTGCATAAACCATTTGCCCGCATTTATTGGTAG
CCTCCTTTTTTATAAAACGCATACTATCTGTTGGTAATGTCGACATTAAT
ACTGGTGTGGTTTTCCTCGTCCATACTGGAGTTGACCTAATTGTGCCATT
CGATTTATAATTTATATTGAAATTATCGGGGCTTTCTCTAAACTGTTCTT
CACTTAATTCTTTGAAATTCATAGTTTTCAAATGCGCTAAATTGCATACA
TTAGTTTTAACATCAAATACTAGAATGGATTCTCCGGTTTGACAATTACT
TTGTCTCATATTACAAAACGGATGCGTTTGAAGTTTTCCTGTTTCATCAT
CAAAAACTCCTTCAAATTCTCTCAATTCTGTCAAATATTCTTCAATCACC
ACTGATTGTTTAATAATTTTCCCATTCACTTTATATAGTTCTCATTCGCA
CGTTCCATCTTGTTGTATTACTCCCTTTACAACAACTGTCTCTCTTATTC
TGTGATTTACTTTTGCTTTCAATTTAATTTCCGGGTTAATCTTTAACACC
TCTGTTTTTAACGCAGCTAAGCAGTTTTCTGGCATTACCTCTACTGGTCG
TGGAATAGCGTGCATTTTTATCAATGATGTGTGGCTCCATTTACCACAGA
AAAATACAAAAGTAGTTCGCTTCGCAATACATTCTCTAACTTTGGCTCGC
CGTAGATCAGATTCCTGATACACATAATATTTTACATCATTTTCTACTTT
CAATTTCTTTGGGTATGCATCCGGACATTCTGCCATGTCCACAACCGAAT
AAATTTTTCCCATTTCTGTTTGGGAACAATCGTAAGCTGTCATACCTGTA
GCTTGTGTAGTTATTGTACAAAAAATAAAGAGCATAACTATTGCCCATGG
GAATATCATCTTTTTTCTTAGCTTCGGGCTATCCTTTCCATTTAACGGTT
TGTATGGTAATTTTTTTTCATCACTTTCATTTACTGTAAGATCATTATTC
AACTTCTTCCAACCTCCATTCTTTTTAACCCACTCTATAAAATATTCATG
GGAGATTCGTACTCCTCCTTCTATCGAATTTTCCAAATTATCCGTTTCTG
CTATCACTTGAGCTCCAATTTCTAAAAACCATATAAGTTAAACTTGTTAA
CATTACTTCTTTGTCAATTCCATTTTTAATCAAATTTTCAATTAGTTTCT
TATCACAAAAGTTATCGTTTTCTCCAAATCTTTGCACAACACCTGGAAGT
CTGTTTTTCATTTCAGTTACTATATTTCCTAACTCATACTCGACATCCAT
TTTTATTTCAAACGTCGGATATACTGCTTTAATCAAGCCCATTAAGATTA
TTTTTGTTTCATCAACTTCCTGTTCAATTTTCACTAAACTCTGCTTTATC
TCGTATAAGGTTCTAGGTTTACTAACAATTATTTCACTATTTATTTCACC
TCGGGGTGCCTCAAAAATTTCTTGTTTTTCAAGGTGTTCCACCTGAATTT
CCTTATTACTGAAAATTTCATCTACTTTTGTTTTCACGTTATCTTCCTCT
ATATCTGTATTTGAATTCTCCGAGTTATCCACTGAATCTACTTTCTGTTC
CTTTATTGCTACTACACAATTTACTAAATACCCTGCCCTTCCATCAGCAT
CTAGGTATCCGTTTTCGTCTAATTCCTGTGGCTTAAATCCACAATCATAT
AAATATTCATCATCATCTAATTCTTCATCTTTGAACATCAATACAATAAT
TCCATCTTTTCCTATTTGGTTTGCAGCTCTTTTTCGTAACTTCTGAACCT
TTGTACACCAATGTTCCTCTATATACTCTGTTTTTCCGCAATCTTCATAA
TCTATTGCTATAATAAATGGTTTTAATTCCATTCTAGTTTCTCTTAATTC
TCTACCAAATCTTCGCATCAAACATTTCAATTTTTCTCCATACCTCGATT
TATCATATAAATATGGTCGTATTATGTTTACTATTTCTAGTTTAGCATTT
TCCTGTAGTTCTATTTTATCAAGTAATTTTAATTTCTCCGTAATTTTAAC
TCCATTCCAATCACTTAAAGCATCTCTTAAGTCTCTTGTTATTTCTTTTG
AATCACTCTTTTCCGCTACTTCTTCGCTAATCATACACGCAACCTGCAAT
TTATTTTTATTTCCAGGTATAAAACCACAATCTATTAAAAATTCATCGTC
TTCTAAATTCATATTTTCTCACTTTAATGATTGCTTTCCTC
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protein sequence of SMED30004174-orf-1

>SMED30004174-orf-1 ID=SMED30004174-orf-1|Name=SMED30004174-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=106bp
MISKFFIKNKLFNIFDIFVRRNHTLKMELVDIITHSVNYSEKFFMLLKTI
FSPNKFLLYDVNKNGLLERSWYRSFSILVIYTPDINVYDKELMKINPFVM
GKKLY*
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protein sequence of SMED30004174-orf-2

>SMED30004174-orf-2 ID=SMED30004174-orf-2|Name=SMED30004174-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
MIFPWAIVMLFIFCTITTQATGMTAYDCSQTEMGKIYSVVDMAECPDAYP
KKLKVENDVKYYVYQESDLRRAKVRECIAKRTTFVFFCGKWSHTSLIKMH
AIPRPVEVMPENCLAALKTEVLKINPEIKLKAKVNHRIRETVVVKGVIQQ
DGTCE*
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protein sequence of SMED30004174-orf-3

>SMED30004174-orf-3 ID=SMED30004174-orf-3|Name=SMED30004174-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=375bp
MNLEDDEFLIDCGFIPGNKNKLQVACMISEEVAEKSDSKEITRDLRDALS
DWNGVKITEKLKLLDKIELQENAKLEIVNIIRPYLYDKSRYGEKLKCLMR
RFGRELRETRMELKPFIIAIDYEDCGKTEYIEEHWCTKVQKLRKRAANQI
GKDGIIVLMFKDEELDDDEYLYDCGFKPQELDENGYLDADGRAGYLVNCV
VAIKEQKVDSVDNSENSNTDIEEDNVKTKVDEIFSNKEIQVEHLEKQEIF
EAPRGEINSEIIVSKPRTLYEIKQSLVKIEQEVDETKIILMGLIKAVYPT
FEIKMDVEYELGNIVTEMKNRLPGVVQRFGENDNFCDKKLIENLIKNGID
KEVMLTSLTYMVFRNWSSSDSRNG*
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protein sequence of SMED30004174-orf-4

>SMED30004174-orf-4 ID=SMED30004174-orf-4|Name=SMED30004174-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=309bp
MRQSNCQTGESILVFDVKTNVCNLAHLKTMNFKELSEEQFRESPDNFNIN
YKSNGTIRSTPVWTRKTTPVLMSTLPTDSMRFIKKEATNKCGQMVYATNY
RGVYVSKMMVHEAKTKIHTNDIKLTHYFNNKIDYLYHFSQNIMENIYREM
ILNDCKLNKEILRNRMAMAITNPNLVAPMLISENGVFARIFGEALYSYKC
KAITVKIMKYEKCTNELPVSINGKMRFMEPITRILLDEQTKPKILQCNRL
LAPLYRLAQDSWIALPDYRGVRTPDKLELTNLEYLVNFTSLNKMAQMGFY
ASENIEEA*
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protein sequence of SMED30004174-orf-5

>SMED30004174-orf-5 ID=SMED30004174-orf-5|Name=SMED30004174-orf-5|organism=Schmidtea mediterranea sexual|type=polypeptide|length=121bp
MIDTILPNGEGKVNYEYLLTPDHFRKATYKTMKKIWGKFMFLGQACAGLI
GFYVVIITIKVIMSQILSAHRIYVISGWSWKIIIGCFPFLARFFITHHQY
REMTSVRAQLKTDQKSELSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR013809ENTH
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0003824catalytic activity
GO:0004077biotin-[acetyl-CoA-carboxylase] ligase activity
GO:0004078biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity
GO:0004079biotin-[methylmalonyl-CoA-carboxytransferase] ligase activity
GO:0004080biotin-[propionyl-CoA-carboxylase (ATP-hydrolyzing)] ligase activity
GO:0005515protein binding
GO:0005524ATP binding
GO:0009374biotin binding
GO:0016874ligase activity
GO:0018271biotin-protein ligase activity
GO:0019899enzyme binding
GO:0042803protein homodimerization activity
Vocabulary: cellular component
TermDefinition
GO:0000785chromatin
GO:0005652nuclear lamina
GO:0005737cytoplasm
GO:0005739mitochondrion
GO:0005829cytosol
GO:0016363nuclear matrix
Vocabulary: biological process
TermDefinition
GO:0006464cellular protein modification process
GO:0006768biotin metabolic process
GO:0008152metabolic process
GO:0008283cell proliferation
GO:0009305protein biotinylation
GO:0016570histone modification
GO:0070781response to biotin
GO:0071110histone biotinylation