Epididymal secretory protein E1

Overview


Name	Epididymal secretory protein E1
Smed ID	SMED30004173
Length (bp)	604

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Epididymal secretory protein E1 (SMED30004173) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Epididymal secretory protein E1 (SMED30004173) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30004173

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
prepharyngeal region	SMED30004173	h1SMcG0004297	SmedASXL_019193	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parapharyngeal region	SMED30004173	h1SMcG0004297	SmedASXL_019193	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parapharyngeal region	SMED30004173	h1SMcG0004297	dd_Smed_v6_4411_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30004173	h1SMcG0004297	OX_Smed_1.0.20039	ox_Smed_v2	PMID:24238224 Kao et al., 2013
head region	SMED30004173	h1SMcG0020096	OX_Smed_1.0.20039	ox_Smed_v2	PMID:24238224 Kao et al., 2013

Note: Hover over icons to view figure legend

Homology

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BLAST of Epididymal secretory protein E1 vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 89.7373 bits (221), Expect = 2.182e-22
Identity = 49/129 (37.98%), Postives = 73/129 (56.59%), Query Frame = 1

Query:  166 VEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAA 552
             EP  + DCGS  G ++ V +SPC T PCQL KG   S+ + + SN    S K  VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D +   + C + P +
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVS 146

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 88.9669 bits (219), Expect = 3.975e-22
Identity = 49/127 (38.58%), Postives = 72/127 (56.69%), Query Frame = 1

Query:  166 VEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
             EP  + DCGS  G ++ V +SPC T PCQL KG   S+ + + SN    S K  VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D +   + C + P
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 89.7373 bits (221), Expect = 6.674e-22
Identity = 49/127 (38.58%), Postives = 72/127 (56.69%), Query Frame = 1

Query:  166 VEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
             EP  + DCGS  G ++ V +SPC T PCQL KG   S+ + + SN    S K  VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D +   + C + P
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 88.9669 bits (219), Expect = 7.392e-22
Identity = 49/127 (38.58%), Postives = 72/127 (56.69%), Query Frame = 1

Query:  166 VEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
             EP  + DCGS  G ++ V +SPC T PCQL KG   S+ + + SN    S K  VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D +   + C + P
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Human
Match: NPC2 (NPC intracellular cholesterol transporter 2 [Source:HGNC Symbol;Acc:HGNC:14537])

HSP 1 Score: 89.7373 bits (221), Expect = 1.326e-21
Identity = 49/127 (38.58%), Postives = 72/127 (56.69%), Query Frame = 1

Query:  166 VEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
             EP  + DCGS  G ++ V +SPC T PCQL KG   S+ + + SN    S K  VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D +   + C + P
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Fly
Match: Npc2a (gene:FBgn0031381 transcript:FBtr0077816)

HSP 1 Score: 80.8777 bits (198), Expect = 2.305e-19
Identity = 53/148 (35.81%), Postives = 78/148 (52.70%), Query Frame = 1

Query:  112 IQMYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTN--PCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPA 549
            ++  +I+  AL V    F     + DCGSK G+   V I  C+T    C L + T  S +I +   E+ ++ K  VHGKV    +PFPL + D C  S   CP++   +  YT T+ + + YP + V + WEL D  G DI+CV+ PA
Sbjct:    2 LRYAVIACAALVV----FAGALEFSDCGSKTGKFTRVAIEGCDTTKAECILKRNTTVSFSIDFALAEEATAVKTVVHGKVLGIEMPFPLANPDACVDSGLKCPLEKDESYRYTATLPVLRSYPKVSVLVKWELQDQDGADIICVEIPA 145

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Fly
Match: Npc2b (gene:FBgn0038198 transcript:FBtr0082888)

HSP 1 Score: 51.6026 bits (122), Expect = 3.022e-8
Identity = 38/128 (29.69%), Postives = 54/128 (42.19%), Query Frame = 1

Query:  196 SKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDN----------DLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPA 549
            SK      V IS C  + C L + T+ SI +K +   D       + G   I +VP P P            D        CP+K+    TY N+ +I  +YP++ + I W L D  G D  C + PA
Sbjct:   31 SKALAAGDVSISNCPKSKCILKRNTEASIQMKIRPERDFQELTSDIQGI--ILDVPLPFPGYYGTSACPHIYDEAGEKKVGCPLKAGQVYTYKNSFKILPVYPTVSLEIHWGLGDKHG-DAACFQIPA 155

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Fly
Match: Npc2b (gene:FBgn0038198 transcript:FBtr0082889)

HSP 1 Score: 51.6026 bits (122), Expect = 3.022e-8
Identity = 38/128 (29.69%), Postives = 54/128 (42.19%), Query Frame = 1

Query:  196 SKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDN----------DLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPA 549
            SK      V IS C  + C L + T+ SI +K +   D       + G   I +VP P P            D        CP+K+    TY N+ +I  +YP++ + I W L D  G D  C + PA
Sbjct:   31 SKALAAGDVSISNCPKSKCILKRNTEASIQMKIRPERDFQELTSDIQGI--ILDVPLPFPGYYGTSACPHIYDEAGEKKVGCPLKAGQVYTYKNSFKILPVYPTVSLEIHWGLGDKHG-DAACFQIPA 155

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BLAST of Epididymal secretory protein E1 vs. Ensembl Zebrafish
Match: zgc:193725 (zgc:193725 [Source:ZFIN;Acc:ZDB-GENE-080723-11])

HSP 1 Score: 97.8265 bits (242), Expect = 1.189e-25
Identity = 52/141 (36.88%), Postives = 83/141 (58.87%), Query Frame = 1

Query:  124 LISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            ++++I LS ++++  +P  ++DCGS  G+V  V+I PC+  PCQL KG   ++ + + S+    + K  VHG V    VPFP+P +D CK S   CPI  Q    Y   + ++  YP+++V + WEL D    D+ C+KFP
Sbjct:    5 VLAVILLSFLAYTCADPVKFVDCGSVHGKVVQVDIKPCSQQPCQLHKGQSYTVNVTFISSVASQTSKAVVHGVVECVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Zebrafish
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:282673])

HSP 1 Score: 96.2857 bits (238), Expect = 4.644e-25
Identity = 49/141 (34.75%), Postives = 82/141 (58.16%), Query Frame = 1

Query:  124 LISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            ++ ++ LS ++++  +P  ++DCGS  G+V  V+I PC+  PC+L KG   ++ + + S  +  + K  VHG +    VPFP+P +D CK S   CPI  Q    Y   + ++  YP+++V + WEL D    D+ C+KFP
Sbjct:    5 VLGVVLLSFLAYTCADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTSKAVVHGVLAGVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFP 144

[Close]

Query:  124 LISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            ++ ++ LS ++++  +P  ++DCGS  G+V  V+I PC+  PC+L KG   ++ + + S  +  + K  VHG +    VPFP+P +D CK S   CPI  Q    Y   + ++  YP+++V + WEL D    D+ C+KFP
Sbjct:    5 VLGVVLLSFLAYTCADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTSKAVVHGVLAGVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Xenopus
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:493351])

HSP 1 Score: 80.8777 bits (198), Expect = 5.121e-19
Identity = 46/129 (35.66%), Postives = 71/129 (55.04%), Query Frame = 1

Query:  160 SFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            S  EP  Y DCGS+ G++ ++++SPC   PC LV+G+  ++   + SN +  S    VHG +    VPFP+ + D CK S  +CPI S    TY   + I+  YP +++ + W+L D    D+ C   P
Sbjct:   23 SVPEPLKYKDCGSQSGKLVTLDVSPCPEEPCPLVRGSTYTVNATFVSNVNSKSASAVVHGIIAGIAVPFPISEPDGCK-SGISCPINSGQTYTYVTKLPIKSEYPCIKLVVKWQLQDENNKDLFCWLIP 150

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Mouse
Match: Npc2 (NPC intracellular cholesterol transporter 2 [Source:MGI Symbol;Acc:MGI:1915213])

HSP 1 Score: 92.4337 bits (228), Expect = 1.287e-23
Identity = 50/129 (38.76%), Postives = 75/129 (58.14%), Query Frame = 1

Query:  160 SFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            S  EP  + DCGSK G ++ V +SPC T+PCQL KG   S+ I + S     +    VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D + N++ C + P
Sbjct:   17 SQAEPLHFKDCGSKVGVIKEVNVSPCPTDPCQLHKGQSYSVNITFTSGTQSQNSTALVHGILEGIRVPFPIPEPDGCK-SGINCPIQKDKVYSYLNKLPVKNEYPSIKLVVEWKLEDDKKNNLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. UniProt/SwissProt
Match: sp|Q9DGJ3|NPC2_DANRE (NPC intracellular cholesterol transporter 2 OS=Danio rerio OX=7955 GN=npc2 PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 3.506e-24
Identity = 49/141 (34.75%), Postives = 82/141 (58.16%), Query Frame = 1

Query:  124 LISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            ++ ++ LS ++++  +P  ++DCGS  G+V  V+I PC+  PC+L KG   ++ + + S  +  + K  VHG +    VPFP+P +D CK S   CPI  Q    Y   + ++  YP+++V + WEL D    D+ C+KFP
Sbjct:    5 VLGVVLLSFLAYTCADPVKFVDCGSVDGKVVQVDIKPCSQQPCKLHKGQSYTVNVTFSSGVESQTSKAVVHGVLAGVPVPFPIPIDDGCK-SGIQCPIVPQKPYNYVTELPVKTEYPAIKVVVEWELRDDSSKDLFCIKFP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. UniProt/SwissProt
Match: sp|P79345|NPC2_BOVIN (NPC intracellular cholesterol transporter 2 OS=Bos taurus OX=9913 GN=NPC2 PE=1 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 6.058e-23
Identity = 50/145 (34.48%), Postives = 80/145 (55.17%), Query Frame = 1

Query:  112 IQMYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            ++   ++ + L++ + +  EP  + DCGS  G ++ V +SPC T PC+L +G   S+ + + SN    S K  VHG V    VPFP+P++D CK S   CPI+      Y N + ++  YPS++V + WEL D +     C + P
Sbjct:    1 MRFLTVAFLFLALSASALAEPVKFKDCGSWVGVIKEVNVSPCPTQPCKLHRGQSYSVNVTFTSNTQSQSSKAVVHGIVMGIPVPFPIPESDGCK-SGIRCPIEKDKTYNYVNKLPVKNEYPSIKVVVEWELTDDKNQRFFCWQIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. UniProt/SwissProt
Match: sp|Q9Z0J0|NPC2_MOUSE (NPC intracellular cholesterol transporter 2 OS=Mus musculus OX=10090 GN=Npc2 PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 9.002e-23
Identity = 50/129 (38.76%), Postives = 75/129 (58.14%), Query Frame = 1

Query:  160 SFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            S  EP  + DCGSK G ++ V +SPC T+PCQL KG   S+ I + S     +    VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D + N++ C + P
Sbjct:   17 SQAEPLHFKDCGSKVGVIKEVNVSPCPTDPCQLHKGQSYSVNITFTSGTQSQNSTALVHGILEGIRVPFPIPEPDGCK-SGINCPIQKDKVYSYLNKLPVKNEYPSIKLVVEWKLEDDKKNNLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. UniProt/SwissProt
Match: sp|P61917|NPC2_PANTR (NPC intracellular cholesterol transporter 2 OS=Pan troglodytes OX=9598 GN=NPC2 PE=1 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.909e-21
Identity = 49/127 (38.58%), Postives = 72/127 (56.69%), Query Frame = 1

Query:  166 VEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
             EP  + DCGS  G ++ V +SPC T PCQL KG   S+ + + SN    S K  VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D +   + C + P
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. UniProt/SwissProt
Match: sp|P61918|NPC2_MACFA (NPC intracellular cholesterol transporter 2 OS=Macaca fascicularis OX=9541 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.909e-21
Identity = 49/127 (38.58%), Postives = 72/127 (56.69%), Query Frame = 1

Query:  166 VEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
             EP  + DCGS  G ++ V +SPC T PCQL KG   S+ + + SN    S K  VHG +    VPFP+P+ D CK S  NCPI+     +Y N + ++  YPS+++ + W+L D +   + C + P
Sbjct:   19 AEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCK-SGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. TrEMBL
Match: A0A0X3NXW4 (Epididymal secretory protein E1 OS=Schistocephalus solidus OX=70667 GN=NPC2 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.274e-30
Identity = 59/121 (48.76%), Postives = 86/121 (71.07%), Query Frame = 1

Query:  187 DCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPA 549
            DCGSK G   +VE+ PC TNPC L KG+  ++T+ +K++  V SGK SVHG +    +PFP+PDNDLCKF++P+CP+ + T  TY  ++E++K YPS+ + I WEL +  G+  +CV+FPA
Sbjct:   55 DCGSKSGTFSAVEVVPCETNPCTLYKGSHANLTLTFKADHTVKSGKASVHGIIAGVPIPFPIPDNDLCKFASPSCPLDAGTTYTYMYSLEVKKTYPSISLHIRWELKEEDGDFHICVQFPA 175

[Close]

BLAST of Epididymal secretory protein E1 vs. TrEMBL
Match: A0A267EQ39 (ML domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025232g1 PE=4 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 9.937e-28
Identity = 53/127 (41.73%), Postives = 82/127 (64.57%), Query Frame = 1

Query:  169 EPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPA 549
            +P  + DCGSK G++ SV+I+PC   PC+L +GTK  +T+ + S+E  ++ K  VHG +    VPFPLPD + C   +P CP+ +  +  YTN +E+++ YP+L + + WEL D   ND+VC + PA
Sbjct:   20 DPVKFKDCGSKVGKINSVDINPCPKLPCELKRGTKVGVTVDFTSSESATAVKSVVHGVISGIAVPFPLPDPNACNAMSPKCPLSNGASYKYTNALEVQQAYPTLALVVRWELTDSNNNDLVCFEVPA 146

[Close]

BLAST of Epididymal secretory protein E1 vs. TrEMBL
Match: W5MY41 (Niemann-Pick disease, type C2 OS=Lepisosteus oculatus OX=7918 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 3.998e-27
Identity = 53/145 (36.55%), Postives = 91/145 (62.76%), Query Frame = 1

Query:  112 IQMYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            ++  + +L+ +S+ +++  EP  ++DCGSK+G++ ++E++PC   PC+L KG   S+ + + S  +  + K  VHG V    VPFP+P++D CK S   CPI+ Q   +Y N + ++  YP +++ + WEL D   ND+ CVKFP
Sbjct:    1 MEYRVAALLFVSLFAFTAAEPVTFIDCGSKEGKILTIEVNPCPKQPCELKKGQSYSVNVTFNSEIESKTSKAVVHGVVAGLPVPFPIPNDDGCK-SGIECPIQKQKTYSYVNQLPVKAEYPCIKLVVEWELRDDGNNDLFCVKFP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. TrEMBL
Match: A0A3B4TDC2 (Niemann-Pick disease, type C2 OS=Seriola dumerili OX=41447 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 3.418e-26
Identity = 54/138 (39.13%), Postives = 84/138 (60.87%), Query Frame = 1

Query:  133 LIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            +++L +I ++   P  YLDCGS  G+V  V+++PCNT PCQL KG   S+ + + S  +  + K  VHG +    VPFP+P  D CK S   CPIK Q +  Y N++ ++  YP++++ + WEL D    D+ C++FP
Sbjct:    8 IVSLCLIGFTCAAPVKYLDCGSTSGKVAVVDVTPCNTQPCQLHKGQSYSVNVTFNSAVESQTSKAVVHGIIAGVPVPFPIPIEDGCK-SGIQCPIKKQQSYHYLNSLPVKSEYPAIKLVVEWELRDDNKQDLFCIRFP 144

[Close]

BLAST of Epididymal secretory protein E1 vs. TrEMBL
Match: A0A267G469 (ML domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig027630g2 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 5.552e-26
Identity = 52/125 (41.60%), Postives = 80/125 (64.00%), Query Frame = 1

Query:  175 TVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPA 549
            T ++DCGSK G++ S++++PC   PC+L +GT   I I +  +E+ +S K  VHG V    VPFPLPD + C    P CP+K+ T   YT+T++++  YP++ V + WEL D +  D VC++ PA
Sbjct:   22 TKFVDCGSKAGKIDSLDVTPCPKIPCELPRGTNAQIKITFTPSENSTSVKAVVHGIVAKIPVPFPLPDTNACDSMAPACPLKAGTQAVYTSTLQVKSSYPAVGVVVKWELADAKEVDFVCLEVPA 146

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Cavefish
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:282673])

HSP 1 Score: 102.449 bits (254), Expect = 1.552e-27
Identity = 54/145 (37.24%), Postives = 87/145 (60.00%), Query Frame = 1

Query:  112 IQMYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            +   LI  + L +++++  E   + DCGS +G+V  V+ISPC T PC+L KG   ++ + + SN D  + K  VHG +    VP+P+P+ D CK S   CPI+ Q + +Y N + ++  YPS+++ + WEL D    D+ C+KFP
Sbjct:    1 MDFRLICAVLLPLLAFTRAEQVKFADCGSVEGKVSQVDISPCPTQPCELHKGKSYTVNVTF-SNVDSQTSKALVHGVIAGVPVPYPIPNEDGCK-SGIQCPIQKQKSYSYINELPVKAEYPSIKLVVEWELRDDSSKDLFCIKFP 143

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Cavefish
Match: npc2 (Niemann-Pick disease, type C2 [Source:NCBI gene;Acc:282673])

HSP 1 Score: 91.2781 bits (225), Expect = 2.281e-23
Identity = 47/118 (39.83%), Postives = 73/118 (61.86%), Query Frame = 1

Query:  193 GSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            GS +G+V  V+ISPC T PC+L KG   ++ + + S+ D  + K  VHG +    VP+P+P+ D CK S   CPI+ Q + +Y N + ++  YPS+++ + WEL D    D+ C+KFP
Sbjct:   15 GSVEGKVSQVDISPCPTQPCELHKGKSYTVNVTFSSDVDSQTSKALVHGVIAGVPVPYPIPNEDGCK-SGIQCPIQKQKSYSYINELPVKAEYPSIKLVVEWELRDDSSKDLFCIKFP 131

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008656.1 (pep scaffold:Pmarinus_7.0:GL477843:35666:38308:1 gene:ENSPMAG00000007827.1 transcript:ENSPMAT00000008656.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.4925 bits (197), Expect = 7.668e-20
Identity = 43/120 (35.83%), Postives = 66/120 (55.00%), Query Frame = 1

Query:  193 GSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAA 552
            GS+ G++ +V I PC T PC L K    +I + + S+ +       VHG +    +PFP+P+ND CK S   CPI       Y   + ++  YPS+++ + WEL D + ND+ C +FP A
Sbjct:   21 GSQTGKISAVNIIPCPTQPCILCKDKTYNINVTFTSDTESQGSTAVVHGILAGIPIPFPIPNNDGCK-SGITCPIAKNQAYIYDVGLPVKSDYPSIKLVVKWELRDDKANDLFCFEFPVA 139

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Nematostella
Match: EDO30774 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZV1])

HSP 1 Score: 90.8929 bits (224), Expect = 1.652e-23
Identity = 54/141 (38.30%), Postives = 84/141 (59.57%), Query Frame = 1

Query:  133 LIALSVISWSFVEPTV-YLDCGSKKGQVQSVE--ISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            L+ L+ +  + V   V + DC   KG+ + VE  ISPC T PC L KGT  S+ I +  +  + SGK  VHG +    VPFPLP+ D+CK S   CP++  T   Y +++E++ +YPSL++ + WE+ D +  D++C + P
Sbjct:    3 LVLLACLVAAAVSSKVDFKDCSGGKGEGEIVELDISPCPTQPCTLHKGTTVSVNITFVPHVTLDSGKAIVHGVIAGIPVPFPLPNADVCKNSGLKCPLEPGTKYVYQSSLEVKTMYPSLKLVVRWEIQDNKNKDVLCFEIP 143

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Nematostella
Match: EDO35255 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM29])

HSP 1 Score: 77.7962 bits (190), Expect = 1.641e-18
Identity = 46/139 (33.09%), Postives = 78/139 (56.12%), Query Frame = 1

Query:  130 SLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            + I L ++  +  +   + DCGSKKG++ SV+++PC+++PC + +G   S  I +  +E V+S KV  +   G+  VP PLP++D CK     CP+KS   V       I + +P+  +T+  EL D   + ++C K P
Sbjct:    7 AAILLFLLCEARAKKVKFQDCGSKKGELISVDLTPCSSDPCVIKRGANASGVITFIPHEVVTSSKVLAYAIFGLIPVPLPLPNSDGCKGYGLTCPLKSGKQVELVFEHYIDQTFPTGHLTLKAELKDQDSDVVICGKIP 145

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Nematostella
Match: EDO43439 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYC7])

HSP 1 Score: 59.3066 bits (142), Expect = 1.215e-11
Identity = 33/117 (28.21%), Postives = 62/117 (52.99%), Query Frame = 1

Query:  181 YLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIV 531
            Y DCGS++G++  ++ISPC++ PC L +GT    ++ +  +ED+   K+S H  +    +P P+P +D C+    +CP+ S     +     I   +P   +T+   + D   + +V
Sbjct:   12 YRDCGSQEGEIVGMDISPCDSEPCVLKRGTSVDGSLTFIPHEDLKRAKLSAHAIIDKLPLPLPIP-SDACQGYGLSCPVDSGVKSMFKIHQAIESEFPVGNLTLKAAVTDSDTSQVV 127

[Close]

BLAST of Epididymal secretory protein E1 vs. Ensembl Medaka
Match: npc2 (NPC intracellular cholesterol transporter 2 [Source:NCBI gene;Acc:111946828])

HSP 1 Score: 87.8113 bits (216), Expect = 6.274e-22
Identity = 42/122 (34.43%), Postives = 69/122 (56.56%), Query Frame = 1

Query:  181 YLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            ++DCGS  G+V  V+I+PC   PCQL +G   ++ + + S+ +  +    VHG +    +PFP+P+ D CK S   CPI       Y   + ++  YP +++ + WEL D   ND+ C++FP
Sbjct:   25 FVDCGSSSGKVAMVDINPCTAQPCQLHRGDSYTVNVTFNSDVESQTSTAVVHGIIAGVPIPFPIPEPDGCK-SGIQCPIHKGQAYNYATQLPVKSEYPPIKLVVEWELKDDNKNDLFCIRFP 145

[Close]

BLAST of Epididymal secretory protein E1 vs. Planmine SMEST
Match: SMESG000031800.1 (SMESG000031800.1)

HSP 1 Score: 265.774 bits (678), Expect = 5.068e-92
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1

Query:  118 MYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAA 552
            MYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDVSSGKVSVHGKVGIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAA
Sbjct:    1 MYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDVSSGKVSVHGKVGIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAA 145

[Close]

BLAST of Epididymal secretory protein E1 vs. Planmine SMEST
Match: SMESG000031788.1 (SMESG000031788.1)

HSP 1 Score: 162.155 bits (409), Expect = 3.844e-51
Identity = 84/145 (57.93%), Postives = 108/145 (74.48%), Query Frame = 1

Query:  118 MYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAA 552
            M +I     ++ +     PTVYLDCGSK G+V+SV+I+PCN  PCQL KGT P +TIKYK+ ED  SG VSVHGK+G   VPFPLPD+DLCKFS+P CPI +   VTY+N+I +   YP++R+T+ WELVD QGND++C+ FPAA
Sbjct:    1 MIVIVFAVFTIFNTISAAPTVYLDCGSKGGKVESVDITPCNEQPCQLKKGTTPQLTIKYKALEDTKSGTVSVHGKIGPIFVPFPLPDSDLCKFSSPACPITTGEEVTYSNSITVLSSYPAIRLTVKWELVDDQGNDLICITFPAA 145

[Close]

BLAST of Epididymal secretory protein E1 vs. Planmine SMEST
Match: SMESG000023323.1 (SMESG000023323.1)

HSP 1 Score: 119.013 bits (297), Expect = 4.910e-34
Identity = 62/141 (43.97%), Postives = 92/141 (65.25%), Query Frame = 1

Query:  127 ISLIALSVISWSFVEP-TVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFP 546
            IS + +  IS  F +  T + DCGS KG +++V + PCN+ PC + +G   +I+I + S ED +SGK  VH       +PFPLP+ DLC+F  P CPIK+ ++  Y+ ++ I+KIYPS+R+T+ WEL D    D+VCV+FP
Sbjct:    8 ISFVFIFAISTIFAKDITHFKDCGSIKGSIKTVSVDPCNSEPCTIQRGQNSTISISFVSKEDCTSGKAKVHALKDGIPIPFPLPNPDLCQFLNPKCPIKTGSDYKYSYSLFIKKIYPSIRLTLRWELQDSLEKDLVCVEFP 148

[Close]

BLAST of Epididymal secretory protein E1 vs. Planmine SMEST
Match: SMESG000031796.1 (SMESG000031796.1)

HSP 1 Score: 56.225 bits (134), Expect = 4.128e-10
Identity = 35/139 (25.18%), Postives = 69/139 (49.64%), Query Frame = 1

Query:  124 LISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYP-SLRVTITWELVDGQGNDIVCV 537
            L+  I   +  ++      + +C +    +QSVE+ PC+   C+L+  T+  I +K+ +N +++ GKV++           PL +++LC  S   CPI    +VT+T+       +P  +RV +  +  D +  +I C+
Sbjct:    2 LLYAIYFVLFQYALAFAPNFFECTNNGHVIQSVEVDPCSDRQCKLLSQTEMQIKVKFTANRNINFGKVALTVTALPSIFAMPLKEDNLCLSSDSKCPIAKGADVTHTSKYTFLGGFPDGVRVIMQLKANDEKNKEIYCM 140

[Close]

BLAST of Epididymal secretory protein E1 vs. Planmine SMEST
Match: SMESG000031795.1 (SMESG000031795.1)

HSP 1 Score: 54.299 bits (129), Expect = 2.047e-9
Identity = 34/139 (24.46%), Postives = 69/139 (49.64%), Query Frame = 1

Query:  124 LISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKGTKPSITIKYKSNEDXXXXXXXXXXXXXIFNVPFPLPDNDLCKFSTPNCPIKSQTNVTYTNTIEIRKIYP-SLRVTITWELVDGQGNDIVCV 537
            L+  I   +  ++      + +C      +QSVE+ PC+   C+L+  T+  I +K+ +N +++ GKV++           PL +++LC  S   CPI    +VT+T+       +P  ++V +  ++ D +  +I C+
Sbjct:    2 LLYAIYFVLFQYALAFSPYFFECTDSGPVIQSVEVDPCSDRQCKLLSQTEMQIKVKFIANRNINLGKVALTVTALPRIFAMPLKEDNLCLSSDSKCPIAKGADVTHTSKYTFLGGFPDGVQVNMQLKVNDEKNKEIYCM 140

The following BLAST results are available for this feature:

BLAST of Epididymal secretory protein E1 vs. Ensembl Human
BLAST of Epididymal secretory protein E1 vs. Ensembl Celegans
BLAST of Epididymal secretory protein E1 vs. Ensembl Fly
BLAST of Epididymal secretory protein E1 vs. Ensembl Zebrafish
BLAST of Epididymal secretory protein E1 vs. Ensembl Xenopus
BLAST of Epididymal secretory protein E1 vs. Ensembl Mouse
BLAST of Epididymal secretory protein E1 vs. UniProt/SwissProt
BLAST of Epididymal secretory protein E1 vs. TrEMBL
BLAST of Epididymal secretory protein E1 vs. Ensembl Cavefish
BLAST of Epididymal secretory protein E1 vs. Ensembl Sea Lamprey
BLAST of Epididymal secretory protein E1 vs. Ensembl Yeast
BLAST of Epididymal secretory protein E1 vs. Ensembl Nematostella
BLAST of Epididymal secretory protein E1 vs. Ensembl Medaka
BLAST of Epididymal secretory protein E1 vs. Planmine SMEST

BLAST of Epididymal secretory protein E1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
NPC2	2.182e-22	37.98	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	3.975e-22	38.58	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	6.674e-22	38.58	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	7.392e-22	38.58	NPC intracellular cholesterol transporter 2 [Sourc...	[more]
NPC2	1.326e-21	38.58	NPC intracellular cholesterol transporter 2 [Sourc...	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Epididymal secretory protein E1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Npc2a	2.305e-19	35.81	gene:FBgn0031381 transcript:FBtr0077816	[more]
Npc2b	3.022e-8	29.69	gene:FBgn0038198 transcript:FBtr0082888	[more]
Npc2b	3.022e-8	29.69	gene:FBgn0038198 transcript:FBtr0082889	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
zgc:193725	1.189e-25	36.88	zgc:193725 [Source:ZFIN;Acc:ZDB-GENE-080723-11]	[more]
npc2	4.644e-25	34.75	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]
npc2	4.644e-25	34.75	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
npc2	5.121e-19	35.66	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Npc2	1.287e-23	38.76	NPC intracellular cholesterol transporter 2 [Sourc...	[more]

BLAST of Epididymal secretory protein E1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9DGJ3\|NPC2_DANRE	3.506e-24	34.75	NPC intracellular cholesterol transporter 2 OS=Dan...	[more]
sp\|P79345\|NPC2_BOVIN	6.058e-23	34.48	NPC intracellular cholesterol transporter 2 OS=Bos...	[more]
sp\|Q9Z0J0\|NPC2_MOUSE	9.002e-23	38.76	NPC intracellular cholesterol transporter 2 OS=Mus...	[more]
sp\|P61917\|NPC2_PANTR	1.909e-21	38.58	NPC intracellular cholesterol transporter 2 OS=Pan...	[more]
sp\|P61918\|NPC2_MACFA	1.909e-21	38.58	NPC intracellular cholesterol transporter 2 OS=Mac...	[more]

BLAST of Epididymal secretory protein E1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0X3NXW4	1.274e-30	48.76	Epididymal secretory protein E1 OS=Schistocephalus...	[more]
A0A267EQ39	9.937e-28	41.73	ML domain-containing protein OS=Macrostomum lignan...	[more]
W5MY41	3.998e-27	36.55	Niemann-Pick disease, type C2 OS=Lepisosteus ocula...	[more]
A0A3B4TDC2	3.418e-26	39.13	Niemann-Pick disease, type C2 OS=Seriola dumerili ...	[more]
A0A267G469	5.552e-26	41.60	ML domain-containing protein OS=Macrostomum lignan...	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
npc2	1.552e-27	37.24	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]
npc2	2.281e-23	39.83	Niemann-Pick disease, type C2 [Source:NCBI gene;Ac...	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000008656.1	7.668e-20	35.83	pep scaffold:Pmarinus_7.0:GL477843:35666:38308:1 g...	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Epididymal secretory protein E1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3


Match Name	E-value	Identity	Description
EDO30774	1.652e-23	38.30	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO35255	1.641e-18	33.09	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO43439	1.215e-11	28.21	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Epididymal secretory protein E1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
npc2	6.274e-22	34.43	NPC intracellular cholesterol transporter 2 [Sourc...	[more]

BLAST of Epididymal secretory protein E1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000031800.1	5.068e-92	100.00	SMESG000031800.1	[more]
SMESG000031788.1	3.844e-51	57.93	SMESG000031788.1	[more]
SMESG000023323.1	4.910e-34	43.97	SMESG000023323.1	[more]
SMESG000031796.1	4.128e-10	25.18	SMESG000031796.1	[more]
SMESG000031795.1	2.047e-9	24.46	SMESG000031795.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30004173 ID=SMED30004173|Name=Epididymal secretory protein E1|organism=Schmidtea mediterranea sexual|type=transcript|length=604bp
TGAAAACTTTGGAATGATTTTAGTGTTAATAACTATCAACTAATTGCACT
GGGAACCTAACATCAGAAATTATAGTATAAAACTAAATAATTTAGTTCTA
CATTTATTCATATACAAATGTATCTTATATCATTGATTGCTTTATCGGTC
ATCAGTTGGTCCTTTGTGGAACCAACAGTATATCTTGATTGTGGATCCAA
AAAAGGACAGGTTCAATCTGTGGAAATTTCACCATGCAATACGAACCCCT
GCCAATTGGTTAAAGGAACAAAACCTTCAATTACAATAAAATATAAATCC
AATGAGGATGTTTCATCTGGCAAAGTTTCAGTTCACGGAAAAGTCGGAAT
TTTCAATGTTCCATTTCCTCTTCCCGATAATGATTTGTGTAAATTCTCAA
CTCCAAATTGCCCCATCAAGTCACAAACGAATGTCACTTATACAAATACT
ATTGAAATTCGTAAAATTTATCCCAGTCTTCGTGTGACTATCACTTGGGA
ACTTGTTGATGGTCAAGGCAACGACATCGTATGCGTGAAATTCCCCGCGG
CAATTGTTAGCTAGATGATATCAAATATAAACATTTCTGTTTTTTAGAAA
AAAA

protein sequence of SMED30004173-orf-1

>SMED30004173-orf-1 ID=SMED30004173-orf-1|Name=SMED30004173-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=149bp
MYLISLIALSVISWSFVEPTVYLDCGSKKGQVQSVEISPCNTNPCQLVKG
TKPSITIKYKSNEDVSSGKVSVHGKVGIFNVPFPLPDNDLCKFSTPNCPI
KSQTNVTYTNTIEIRKIYPSLRVTITWELVDGQGNDIVCVKFPAAIVS*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000418	head
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR039670	NPC2-like
IPR033916	ML_Npc2-like
IPR003172	ML_dom
IPR014756	Ig_E-set

Vocabulary: **cellular component**
Term	Definition
GO:0005622	intracellular

Vocabulary: **biological process**
Term	Definition
GO:0032367	intracellular cholesterol transport

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003172	MD-2-related lipid-recognition domain	SMART	SM00737	pgtp_13	coord: 22..144 e-value: 6.5E-36 score: 135.3
IPR003172	MD-2-related lipid-recognition domain	PFAM	PF02221	E1_DerP2_DerF2	coord: 24..146 e-value: 7.3E-28 score: 97.7
None	No IPR available	GENE3D	G3DSA:2.60.40.770		coord: 18..148 e-value: 1.9E-44 score: 153.0
None	No IPR available	PANTHER	PTHR11306:SF30	NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 2	coord: 6..147
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..19 score: 0.69
IPR039670	Sterol transport protein NPC2-like	PANTHER	PTHR11306	NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED	coord: 6..147
IPR033916	Npc2 like, ML domain	CDD	cd00916	Npc2_like	coord: 24..144 e-value: 2.51333E-38 score: 124.729
IPR014756	Immunoglobulin E-set	SUPERFAMILY	SSF81296	E set domains	coord: 20..147

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Epididymal secretory protein E1

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

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