GCR058
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004162 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of GCR058 vs. Ensembl Human
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:HGNC Symbol;Acc:HGNC:4525]) HSP 1 Score: 155.221 bits (391), Expect = 4.857e-42 Identity = 117/357 (32.77%), Postives = 188/357 (52.66%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAV--------YYKIQMKVTNLT----WNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICC-RSIPGNGYLQGASGY 1173
IT + Y+ H V I I Y +IF L ++GN V V+R+ M TV N+FI NL+I+D+L +F +PITLL +I W FG +C+ S +QG +V AS+ TL IA DR+ + +P K + KT ++I++ IW+ + I+ P AV YY++++ N T W C +WPN+ E ++ + V+F +Y+ PL+LI I Y +G+ ++ R + ++G+K N + +K K++KMLL V + F +SWLPL+ ++L Y + + N I+ Y P L N+ +NP IY N+ R + + +C R+ P Y A +
Sbjct: 32 ITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQNKTSPVYW----------CREDWPNQ-EMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLF-RAAVPHTGRK---NQEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYAD---LSPNELQIINIYIYPFAHWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSH 370
BLAST of GCR058 vs. Ensembl Human
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:HGNC Symbol;Acc:HGNC:4525]) HSP 1 Score: 155.221 bits (391), Expect = 5.199e-42 Identity = 117/357 (32.77%), Postives = 188/357 (52.66%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAV--------YYKIQMKVTNLT----WNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICC-RSIPGNGYLQGASGY 1173
IT + Y+ H V I I Y +IF L ++GN V V+R+ M TV N+FI NL+I+D+L +F +PITLL +I W FG +C+ S +QG +V AS+ TL IA DR+ + +P K + KT ++I++ IW+ + I+ P AV YY++++ N T W C +WPN+ E ++ + V+F +Y+ PL+LI I Y +G+ ++ R + ++G+K N + +K K++KMLL V + F +SWLPL+ ++L Y + + N I+ Y P L N+ +NP IY N+ R + + +C R+ P Y A +
Sbjct: 35 ITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQNKTSPVYW----------CREDWPNQ-EMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLF-RAAVPHTGRK---NQEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYAD---LSPNELQIINIYIYPFAHWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSH 373
BLAST of GCR058 vs. Ensembl Human
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:HGNC Symbol;Acc:HGNC:4525]) HSP 1 Score: 154.451 bits (389), Expect = 5.202e-41 Identity = 117/357 (32.77%), Postives = 188/357 (52.66%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAV--------YYKIQMKVTNLT----WNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICC-RSIPGNGYLQGASGY 1173
IT + Y+ H V I I Y +IF L ++GN V V+R+ M TV N+FI NL+I+D+L +F +PITLL +I W FG +C+ S +QG +V AS+ TL IA DR+ + +P K + KT ++I++ IW+ + I+ P AV YY++++ N T W C +WPN+ E ++ + V+F +Y+ PL+LI I Y +G+ ++ R + ++G+K N + +K K++KMLL V + F +SWLPL+ ++L Y + + N I+ Y P L N+ +NP IY N+ R + + +C R+ P Y A +
Sbjct: 134 ITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQNKTSPVYW----------CREDWPNQ-EMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLF-RAAVPHTGRK---NQEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYAD---LSPNELQIINIYIYPFAHWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSH 472
BLAST of GCR058 vs. Ensembl Human
Match: QRFPR (pyroglutamylated RFamide peptide receptor [Source:HGNC Symbol;Acc:HGNC:15565]) HSP 1 Score: 133.65 bits (335), Expect = 4.376e-34 Identity = 95/348 (27.30%), Postives = 168/348 (48.28%), Query Frame = 1
Query: 220 MIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVW--QRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSS 1257
+IF L + GN V V R +MRTV NIFIC+L+++D+L F +P+T+LQ+I + W G +C+ P++Q T V I T+T IA +R+ + HP K + + +++ +++ Q+++ ++ ++ CL EW + K Y I +IL+++PL ++ I Y+ +G ++W +RV D S + I RKK + V M++TVV FA+ W P ++ ++ Y N + + + I Q++G SN+ NP +Y + + + + +C Y + T S + R N G+ + K K S
Sbjct: 54 LIFALALFGNALVFYVVTRSKAMRTVTNIFICSLALSDLLITFFCIPVTMLQNISDNWLGGAFICKMVPFVQSTAVVTEILTMTCIAVERHQGLVHPFKMKWQYTNRRAFTMLGVVWLVAVIVGSPMWHVQQLEI---KYDFLYEKEHICCLEEWTSPVHQKIY-TTFILVILFLLPLMVMLILYSKIGYELWIKKRVG-DGSVLRTIHGKEMSKIARKKK-RAVIMMVTVVALFAVCWAPFHVVHMMIEYSNFEK---EYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVLSAVC---------------YCIVNKTFSPAQRHGNSGITMMRKKAKFS 377
BLAST of GCR058 vs. Ensembl Human
Match: NPFFR1 (neuropeptide FF receptor 1 [Source:HGNC Symbol;Acc:HGNC:17425]) HSP 1 Score: 120.553 bits (301), Expect = 1.712e-29 Identity = 98/329 (29.79%), Postives = 165/329 (50.15%), Query Frame = 1
Query: 169 YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNL---TWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICCRS 1134
Y++H+ V + + Y +IF+L ++GN V V+++ M TV N+FI NL+++D+L +F +P TL+ ++ W F C+ S +QG +V AS+ TL IA +R+ I HP + + + + + IW + +I+ P AV + + + N S+ L+ C WP KG + Y V+F +Y+ PL LI + YA + K+ Q G++ ++ ++V ML+ V +FF +SWLPL+ LLL Y + +++ T+ Y P L N+ NP IY N+ R + A +C R
Sbjct: 35 YYQHTSPVAAMFIVAYALIFLLCMVGNTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALVTIAVIWALALLIMCPSAVTLTVTREEHHFMVDARNRSYPLYS--CWEAWPEKGMRRVY-TTVLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADP-----RASRRRARVVHMLVMVALFFTLSWLPLWALLLLIDYGQLSAPQLHLVTV---YAFPFAHWLAFFNSSANPIIYGYFNENFRRGFQAAFRARLCPRP 352
BLAST of GCR058 vs. Ensembl Celegans
Match: npr-35 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q7YXG8]) HSP 1 Score: 115.546 bits (288), Expect = 2.545e-28 Identity = 88/322 (27.33%), Postives = 157/322 (48.76%), Query Frame = 1
Query: 163 LPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKV--WQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVI 1122
+P +HS + + ++ YG +F ++GN V+L V H ++ + I +L++AD+ LP TL+ SI EW +G CR S + +T AS+ TL + DRY+AICH +K + Y + + + +Y + V GS+ ++C ++ + K YFL V ++ +++P LIS Y + + V + ++ D ++ DR K +++ M+LTV++ FA WLPLY +++ +Q ++ T +L PIFQ + ++ +NP IY YS KY +++
Sbjct: 19 VPLLRHSYQLTVLYTVAYGAVFFTGVLGNTFVVLAVWAHKNLNITTDYLILSLALADLFILWICLPTTLINSIFTEWLWGQFFCRLSTWANASTSFASVYTLVAVTADRYLAICHTLKYNTSWDREYTKYVIFAVWLVAAIFGIPNWYNYDLIVWQ---EGSYGY--RLC----TSQTDQKLYFLFVNLLLAFIVPFGLISGLYTRIFITVSTHRSLAVDARARE----------DRVK-LRVATMMLTVIIVFACCWLPLYCIFTYFFFFADQRSDLFQITSML--IRPIFQWMSLLSSSLNPIIYIAYSHKYRRAFKSIL 318
BLAST of GCR058 vs. Ensembl Celegans
Match: npr-10 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q6EUU1]) HSP 1 Score: 105.145 bits (261), Expect = 8.770e-25 Identity = 76/246 (30.89%), Postives = 127/246 (51.63%), Query Frame = 1
Query: 193 ICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISW 930
I IL+ Y +I V+ + GN+ V+++V+R +++V N+FI +LS++DI A+ +T + + W FG LC P LQGT + S TLT IA DRY+ ICHP K I K ++ S + +P + ++ M+ N + G + C W + + V+FII +V PL I+ YA++ +K+ + V S K+ L ++ ++ +ML+ + V FA+SW
Sbjct: 28 IAILATIYTIIVVVGVTGNLLVVMSVMRFKVLQSVRNMFIVSLSVSDIFVAIVSGSVTPITAFSKVWLFGGPLCHLLPLLQGTALSFSTLTLTAIAIDRYILICHPTKEPIRKDQALKMISFNSAISVGLSVPLFMKQEL-MQFRN--YCGEY------CSENWGPDAYLRSVYGTVVFIIQFVFPLITITFCYASISIKLRRGVFVRGSQKE--LMSEARRQLTQRRLRTNRMLIIMTVTFALSW 262
BLAST of GCR058 vs. Ensembl Celegans
Match: npr-10 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q6EUU1]) HSP 1 Score: 104.76 bits (260), Expect = 1.027e-24 Identity = 76/246 (30.89%), Postives = 127/246 (51.63%), Query Frame = 1
Query: 193 ICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISW 930
I IL+ Y +I V+ + GN+ V+++V+R +++V N+FI +LS++DI A+ +T + + W FG LC P LQGT + S TLT IA DRY+ ICHP K I K ++ S + +P + ++ M+ N + G + C W + + V+FII +V PL I+ YA++ +K+ + V S K+ L ++ ++ +ML+ + V FA+SW
Sbjct: 28 IAILATIYTIIVVVGVTGNLLVVMSVMRFKVLQSVRNMFIVSLSVSDIFVAIVSGSVTPITAFSKVWLFGGPLCHLLPLLQGTALSFSTLTLTAIAIDRYILICHPTKEPIRKDQALKMISFNSAISVGLSVPLFMKQEL-MQFRN--YCGEY------CSENWGPDAYLRSVYGTVVFIIQFVFPLITITFCYASISIKLRRGVFVRGSQKE--LMSEARRQLTQRRLRTNRMLIIMTVTFALSW 262
BLAST of GCR058 vs. Ensembl Celegans
Match: npr-4 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:A0A078BTK0]) HSP 1 Score: 96.6709 bits (239), Expect = 8.579e-22 Identity = 76/235 (32.34%), Postives = 122/235 (51.91%), Query Frame = 1
Query: 247 NIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQ-LHRKICLMEW-PNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDR-----KKSIKLVKMLLTVVVFFAISW 930
N V+L + R+ S++TV N+FI +LS +DI+ IT + + EW FG LCR +P++ G ++C S TLT I+ DRY+ I PM++ I ++ I +A I P + +K ++ G F+ + C W N+ + K Y ++F+ L VIPLT+I I Y A+ LK+ Q + + K+ N D+ K+ + +ML+ +VV FA SW
Sbjct: 45 NSCVILAITRNKSLQTVPNLFILSLSCSDIVVCCTSATITPITAFKKEWIFGEALCRIAPFIAGISLCFSTFTLTAISIDRYILIRFPMRKPITHYQAVGVIAIICAFAATITSP--IMFKQKL--------GEFENFCGQYCTENWGANESQRKIYGAALMFLQL-VIPLTIIIISYTAISLKIGQSMILKGAKKQKTDNWEMELSDQQRIAVKRRQRTNRMLIGMVVAFACSW 268
BLAST of GCR058 vs. Ensembl Celegans
Match: npr-2 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:A0A061AKJ8]) HSP 1 Score: 96.6709 bits (239), Expect = 1.010e-21 Identity = 67/220 (30.45%), Postives = 119/220 (54.09%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVST 813
I + P + V+ S+ Y IF+L I+GN AVL ++H ++TV NIFI NL +++L L LPIT + +++ +W F + +C+ P +QG ++ S +L+ IA DRY + P K+ ++ ++ +I + IW+ S ++ +P+ Y M V L NG L + C WP E ++ + ++ I ++ P ++ Y + ++ QRV T
Sbjct: 13 IDKHPDMTNEPTVLVTFSLLYLHIFLLGILGNSAVLYLTMKHRQLQTVQNIFILNLCASNVLMCLTSLPITFITNVYKQWFFSSPVCKLIPLVQGASIFVSTFSLSAIALDRYNLVVRPHKQKLSSRSAMMIALLIWVISVVVCMPYGWY----MDVEKL--NG---LCGEYCSEHWP-LAEVRKGYTFLVLITQFLFPFATMAFCYYNIFSRLRQRVET 222
BLAST of GCR058 vs. Ensembl Fly
Match: SIFaR (gene:FBgn0038880 transcript:FBtr0084098) HSP 1 Score: 162.54 bits (410), Expect = 2.711e-43 Identity = 110/325 (33.85%), Postives = 190/325 (58.46%), Query Frame = 1
Query: 166 PYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI--QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRS 1134
P ++HS + + + Y ++F++ +IGN V+ V+R MRTV N FI NL+IADIL +F LP TL+ +I W G L+C+ PY+QG +V AS+ +L ++ DR++AI P+K+ + K+ I++IGIW+ + + +PW +++ + +V + ++ + +C WP +G YFL + Y++P++LI++ Y + +KV R S K ++ + +K +K++KML+ VV+ F +SWLPLY+ + ++ S + +L +P+ Q LG+SN+CINP I Y+ ++KY G A+I RS
Sbjct: 199 PLYRHSLAMSMVYCVAYIVVFLVGLIGNSFVIAVVLRAPRMRTVTNYFIVNLAIADILVIVFCLPATLIGNIFVPWMLGWLMCKFVPYIQGVSVAASVYSLIAVSLDRFIAIWWPLKQ-MTKRRARIMIIGIWVIALVTTIPWLLFFDLVPAEEVFSDALVSAYSQPQFLCQEVWPPGTDGNLYFLLANLVACYLLPMSLITLCYVLIWIKVSTR-SIPGESKDAQMDR----MQQKSKVKVIKMLVAVVILFVLSWLPLYVIFARIKFGSDISQE---EFEILKKVMPVAQWLGSSNSCINP-ILYSVNKKYRRGFAAIIKSRS 513
BLAST of GCR058 vs. Ensembl Fly
Match: SIFaR (gene:FBgn0038880 transcript:FBtr0301815) HSP 1 Score: 162.54 bits (410), Expect = 2.711e-43 Identity = 110/325 (33.85%), Postives = 190/325 (58.46%), Query Frame = 1
Query: 166 PYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI--QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRS 1134
P ++HS + + + Y ++F++ +IGN V+ V+R MRTV N FI NL+IADIL +F LP TL+ +I W G L+C+ PY+QG +V AS+ +L ++ DR++AI P+K+ + K+ I++IGIW+ + + +PW +++ + +V + ++ + +C WP +G YFL + Y++P++LI++ Y + +KV R S K ++ + +K +K++KML+ VV+ F +SWLPLY+ + ++ S + +L +P+ Q LG+SN+CINP I Y+ ++KY G A+I RS
Sbjct: 199 PLYRHSLAMSMVYCVAYIVVFLVGLIGNSFVIAVVLRAPRMRTVTNYFIVNLAIADILVIVFCLPATLIGNIFVPWMLGWLMCKFVPYIQGVSVAASVYSLIAVSLDRFIAIWWPLKQ-MTKRRARIMIIGIWVIALVTTIPWLLFFDLVPAEEVFSDALVSAYSQPQFLCQEVWPPGTDGNLYFLLANLVACYLLPMSLITLCYVLIWIKVSTR-SIPGESKDAQMDR----MQQKSKVKVIKMLVAVVILFVLSWLPLYVIFARIKFGSDISQE---EFEILKKVMPVAQWLGSSNSCINP-ILYSVNKKYRRGFAAIIKSRS 513
BLAST of GCR058 vs. Ensembl Fly
Match: TkR86C (gene:FBgn0004841 transcript:FBtr0082315) HSP 1 Score: 124.02 bits (310), Expect = 1.142e-30 Identity = 104/336 (30.95%), Postives = 174/336 (51.79%), Query Frame = 1
Query: 145 FPIITR---LPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPN---KGEGKEYFLGVIFIIL-YVIPLTLISIFYAAVGLKVW-QRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICC 1128
FP TR LP+ + + I +I +G++ + I GN VL V H SMRTV N F+ NLSIAD+L + + ++++W FG++ C + ++ TV S+ TL I+FDRY+AI HP+KR +++ II++ IW S ++ P +Y I +T +NG R +C M WP+ +Y +I ++L Y IP+ ++ I Y+ +G +W R +N+ ++ K K+V+M + +V FAI WLP ++ + Y+ N + V+ + L ++ L SN +NP IYY ++++ +ICC
Sbjct: 69 FPSPTRPYELPWEQKT-----IWAIIFGLMMFVAIAGNGIVLWIVTGHRSMRTVTNYFLLNLSIADLLMSSLNCVFNFIFMLNSDWPFGSIYCTINNFVANVTVSTSVFTLVAISFDRYIAIVHPLKRRTSRRKVRIILVLIWALSCVLSAPCLLYSSI---MTKHYYNGK---SRTVCFMMWPDGRYPTSMADYAYNLIILVLTYGIPMIVMLICYSLMGRVLWGSRSIGENTDRQM--------ESMKSKRKVVRMFIAIVSIFAICWLPYHLFFIYAYHNNQVASTKYVQHMYLGFYW-----LAMSNAMVNPLIYYWMNKRFRMYFQRIICC 380
BLAST of GCR058 vs. Ensembl Fly
Match: TkR86C (gene:FBgn0004841 transcript:FBtr0112789) HSP 1 Score: 123.635 bits (309), Expect = 2.844e-30 Identity = 104/336 (30.95%), Postives = 174/336 (51.79%), Query Frame = 1
Query: 145 FPIITR---LPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPN---KGEGKEYFLGVIFIIL-YVIPLTLISIFYAAVGLKVW-QRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICC 1128
FP TR LP+ + + I +I +G++ + I GN VL V H SMRTV N F+ NLSIAD+L + + ++++W FG++ C + ++ TV S+ TL I+FDRY+AI HP+KR +++ II++ IW S ++ P +Y I +T +NG R +C M WP+ +Y +I ++L Y IP+ ++ I Y+ +G +W R +N+ ++ K K+V+M + +V FAI WLP ++ + Y+ N + V+ + L ++ L SN +NP IYY ++++ +ICC
Sbjct: 69 FPSPTRPYELPWEQKT-----IWAIIFGLMMFVAIAGNGIVLWIVTGHRSMRTVTNYFLLNLSIADLLMSSLNCVFNFIFMLNSDWPFGSIYCTINNFVANVTVSTSVFTLVAISFDRYIAIVHPLKRRTSRRKVRIILVLIWALSCVLSAPCLLYSSI---MTKHYYNGK---SRTVCFMMWPDGRYPTSMADYAYNLIILVLTYGIPMIVMLICYSLMGRVLWGSRSIGENTDRQM--------ESMKSKRKVVRMFIAIVSIFAICWLPYHLFFIYAYHNNQVASTKYVQHMYLGFYW-----LAMSNAMVNPLIYYWMNKRFRMYFQRIICC 380
BLAST of GCR058 vs. Ensembl Fly
Match: Lkr (gene:FBgn0035610 transcript:FBtr0077149) HSP 1 Score: 115.546 bits (288), Expect = 1.490e-27 Identity = 88/316 (27.85%), Postives = 159/316 (50.32%), Query Frame = 1
Query: 190 VICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNG-SFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWN---VNVKTIVLTYFLPIF----QVLGASNNCINPFIYYNYSRKYHDGIN 1113
++ +LSI YG I ++ +IGN V+ V MRTV N++I NL+ AD++ LF +P ++ W+ +C P++Q +V S+ TLT IA DR+ AI +P++ K + I+ GIW+ + + +P+A+ ++++ N ++ + R C+ + + + + + ++F + Y++P +IS Y + +++W + N+ + K K++KML+ VV+ F + WLPL Q +N V + I +F+ I L SN+C NPFIY Y+ K+ N
Sbjct: 34 IVALLSIFYGGISIVAVIGNTLVIWVVATTRQMRTVTNMYIANLAFADVIIGLFCIPFQFQAALLQSWNLPWFMCSFCPFVQALSVNVSVFTLTAIAIDRHRAIINPLRARPTKFVSKFIIGGIWMLALLFAVPFAIAFRVEELTERFRENNETYNVTRPFCMNKNLSDDQLQSFRYTLVF-VQYLVPFCVISFVYIQMAVRLWGTRAPGNAQDSRDITLL------KNKKKVIKMLIIVVIIFGLCWLPL------------QLYNILYVTIPEINDYHFISIVWFCCDWLAMSNSCYNPFIYGIYNEKFKREFN 330
BLAST of GCR058 vs. Ensembl Zebrafish
Match: npffr1l3 (neuropeptide FF receptor 1 like 3 [Source:ZFIN;Acc:ZDB-GENE-070424-44]) HSP 1 Score: 147.902 bits (372), Expect = 2.083e-39 Identity = 101/334 (30.24%), Postives = 181/334 (54.19%), Query Frame = 1
Query: 133 NNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMK-----VTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAV 1119
N+ + I PY++HS + ++ Y IF+L ++GN V + V+R+ MRTV NIFI NL+++D+L +F +P TL+ ++ W F +C+ S +QG +V AS+ TL IA DR+ I +P K + + + IW+ + I+ P + ++ + + N +N ++ L+ C WP+ E ++ + V+FI +YVIPL LI + Y +G K++ ++++ + K+ I++VKML+ V + F +SWLPL+ ++L Y + + ++ + T +Y P+ L SN+ +NP IY ++ + G AV
Sbjct: 31 NSSTMAGIILSPYYQHSLPTAALFTLAYLFIFLLCLMGNALVCVIVLRNRHMRTVTNIFILNLAVSDLLVGIFCIPTTLVDNLITGWPFSNTVCKLSGLVQGMSVSASVFTLVAIAVDRFRCIVYPFKPKLTLFIAKVSIGTIWLLALTIMFPSVLMLTVEQERAHFMIYNDDYNNTYPLYS--CYETWPDP-EMRKIYTTVLFIHIYVIPLALIMLMYGRIGAKLYSAAVSEHANAQ-----------GKRKIRVVKMLIMVALLFMLSWLPLWTLMMLTDYGHPDNDSLEILT---SYIFPLSHWLAFSNSSVNPIIYGYFNENFKRGFQAV 347
BLAST of GCR058 vs. Ensembl Zebrafish
Match: npffr2b (neuropeptide FF receptor 2b [Source:ZFIN;Acc:ZDB-GENE-070705-159]) HSP 1 Score: 141.354 bits (355), Expect = 6.775e-38 Identity = 104/334 (31.14%), Postives = 176/334 (52.69%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI--QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGK-----------KFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINA 1116
IT + ++ H V I + Y +IF++ ++GN V V+R +MRTV N+FI NL+I+D+L +F +P TL+ +I W FG+L+C+ S +QG +V AS+ TL IA DR+ I +P K+ + T+ +I+I IW+ + I+ P V ++ + +V+ L +G+ C WPN+ E ++ + V+F +Y+ PLTLI I YA +G+ +++ + K C + + RKK +++KMLL V + F +SW L + S + ++ YF P+ L N+ +NP IY ++ + G A
Sbjct: 2 ITYVDFYLHEPFVSAIFIVSYLLIFIVCMMGNGVVCFIVLRSKNMRTVTNLFILNLAISDLLVGIFCMPTTLVDNIITGWPFGSLVCKLSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTSTLIIIIIWVLAISIMCPSGVMLQVTKEHRVSILLSDGNTTQPFYWCRENWPNQ-EMRKIYTTVLFANIYLAPLTLIVIMYARIGITLFKTSMPAPVSQVGTSGSGSGSGKQCRYESRQLVSRKKK-RVIKMLLVVALLFILSW---LPLWALMMLSDYASLSERQHRVINIYFYPLAHWLAFCNSSVNPIIYGFFNENFRKGFKA 330
BLAST of GCR058 vs. Ensembl Zebrafish
Match: npffr2b (neuropeptide FF receptor 2b [Source:ZFIN;Acc:ZDB-GENE-070705-159]) HSP 1 Score: 143.665 bits (361), Expect = 6.860e-38 Identity = 103/324 (31.79%), Postives = 174/324 (53.70%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI--QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAV 1119
IT + ++ H V I + Y +IF++ ++GN V V+R +MRTV N+FI NL+I+D+L +F +P TL+ +I W FG+L+C+ S +QG +V AS+ TL IA DR+ I +P K+ + T+ +I+I IW+ + I+ P V ++ + +V+ L +G+ C WPN+ E ++ + V+F +Y+ PLTLI I YA +G+ +++ C + + RKK +++KMLL V + F +SW L + S + ++ YF P+ L N+ +NP IY ++ + G A
Sbjct: 21 ITYVDFYLHEPFVSAIFIVSYLLIFIVCMMGNGVVCFIVLRSKNMRTVTNLFILNLAISDLLVGIFCMPTTLVDNIITGWPFGSLVCKLSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTSTLIIIIIWVLAISIMCPSGVMLQVTKEHRVSILLSDGNTTQPFYWCRENWPNQ-EMRKIYTTVLFANIYLAPLTLIVIMYARIGITLFKTSMP-------CRYESRQLVSRKKK-RVIKMLLVVALLFILSW---LPLWALMMLSDYASLSERQHRVINIYFYPLAHWLAFCNSSVNPIIYGFFNENFRKGFKAA 332
BLAST of GCR058 vs. Ensembl Zebrafish
Match: AL929549.1 (pep chromosome:GRCz11:CHR_ALT_CTG5_1_26:33067396:33103059:1 gene:ENSDARG00000113516.1 transcript:ENSDART00000190287.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL929549.1) HSP 1 Score: 144.05 bits (362), Expect = 7.847e-38 Identity = 103/324 (31.79%), Postives = 174/324 (53.70%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI--QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAV 1119
IT + ++ H V I + Y +IF++ ++GN V V+R +MRTV N+FI NL+I+D+L +F +P TL+ +I W FG+L+C+ S +QG +V AS+ TL IA DR+ I +P K+ + T+ +I+I IW+ + I+ P V ++ + +V+ L +G+ C WPN+ E ++ + V+F +Y+ PLTLI I YA +G+ +++ C + + RKK +++KMLL V + F +SW L + S + ++ YF P+ L N+ +NP IY ++ + G A
Sbjct: 21 ITYVDFYLHEPFVSAIFIVSYLLIFIVCMVGNGVVCFIVLRSKNMRTVTNLFILNLAISDLLVGIFCMPTTLVDNIITGWPFGSLVCKLSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTSTLIIIIIWVLAISIMCPSGVMLQVTKEHRVSILLSDGNTTQPFYWCRENWPNQ-EMRKIYTTVLFANIYLAPLTLIVIMYARIGITLFKTSMP-------CRYESRQLVSRKKK-RVIKMLLVVALLFILSW---LPLWALMMLSDYASLSERQHRVINIYFYPLAHWLAFCNSSVNPIIYGFFNENFRKGFKAA 332
BLAST of GCR058 vs. Ensembl Zebrafish
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110]) HSP 1 Score: 142.51 bits (358), Expect = 3.575e-37 Identity = 104/343 (30.32%), Postives = 172/343 (50.15%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQM-----KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRV-----STDNSGKKFCLNHTCGGIDR------KKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRS 1134
IT PY++HS V L++ Y IF+L ++GN V L V+ + MRTV N+FI NL+++D+L +F +P TL+ ++ W F +C+ S +QG +V AS+ TL IA DR+ I +P + + + ++ IW+ + +I+ P AV ++ V +N ++ L C W N + ++ + V+F +Y+IPLTLI++ Y +G+K++ + G+ GG +K IK++KML V + F ISW L + N + ++ Y P L SN+ +NP IY Y+ + G AV CR+
Sbjct: 35 ITYYPYYQHSLPVAAALTLAYLFIFLLCMVGNGLVCLIVLENRRMRTVTNLFILNLAVSDLLVGVFCIPTTLVDNLITGWPFTNTVCKMSGLVQGMSVSASVFTLVAIAVDRFRCIVYPFQPKLTLLVAKVTIVMIWVLAVVIMCPSAVTLTVERVEHHYMVRGEDYNHTYPLFS--CFENWANP-QMRKVYTTVLFAHIYLIPLTLITLMYGRIGIKLYTTSVISGNDQHDGGQPHTSPQAPGGQQEGRPLISQKKIKVIKMLSIVALLFTISW---LPLWTLMLLTDYGGLNEDELELLSGYVFPFAHWLAFSNSSVNPIIYGYYNENFKRGFQAV--CRA 369
BLAST of GCR058 vs. Ensembl Xenopus
Match: clstn2 (calsyntenin 2 [Source:Xenbase;Acc:XB-GENE-958942]) HSP 1 Score: 142.895 bits (359), Expect = 1.811e-37 Identity = 101/333 (30.33%), Postives = 180/333 (54.05%), Query Frame = 1
Query: 169 YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNL---TWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQ-------RVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVIC 1125
Y++HS + + + Y IF++ +IGN+ V V+++ MRTV N+FI NL+I+D+L +F +P TL+ ++ W F ++C+ S +QG +V AS+ TL IA +R+ I +P ++ + + I +I IW+ + II+ P AV + + +N + L+ C EWP+ E ++ + V+F +Y+ PLTLI I YA + K+++ VS +N G++ K+ ++++ ML+ V +FF++SWLPL+ +LL Y + + +N ++ Y P L N+ +NP IY N+ R + + +C
Sbjct: 29 YYQHSTSIAAMFILAYTFIFLMCMIGNMLVCFIVLKNRQMRTVTNMFILNLAISDLLVGIFCMPTTLVDNLITGWPFDNIMCKMSGLVQGMSVSASVFTLVAIAVERFRCIVYPFRQKLTLRKAIITIIIIWVLALIIMCPSAVTLTVTKDDFHFMVDDYNNYYPLYS--CWEEWPDT-EMRKIYTTVLFSHIYLAPLTLIVIMYARIAFKLFKSSAPIRGSVSEENEGRRVS----------KRKVRVIYMLIIVALFFSLSWLPLWTLMLLTDYGHLSDYQLN---LIAVYVFPFAHWLAFFNSSVNPIIYGYFNENFRRGFQEAFKVQVC 345
BLAST of GCR058 vs. Ensembl Xenopus
Match: clstn2 (calsyntenin 2 [Source:Xenbase;Acc:XB-GENE-958942]) HSP 1 Score: 142.895 bits (359), Expect = 1.879e-37 Identity = 101/333 (30.33%), Postives = 180/333 (54.05%), Query Frame = 1
Query: 169 YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNL---TWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQ-------RVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVIC 1125
Y++HS + + + Y IF++ +IGN+ V V+++ MRTV N+FI NL+I+D+L +F +P TL+ ++ W F ++C+ S +QG +V AS+ TL IA +R+ I +P ++ + + I +I IW+ + II+ P AV + + +N + L+ C EWP+ E ++ + V+F +Y+ PLTLI I YA + K+++ VS +N G++ K+ ++++ ML+ V +FF++SWLPL+ +LL Y + + +N ++ Y P L N+ +NP IY N+ R + + +C
Sbjct: 30 YYQHSTSIAAMFILAYTFIFLMCMIGNMLVCFIVLKNRQMRTVTNMFILNLAISDLLVGIFCMPTTLVDNLITGWPFDNIMCKMSGLVQGMSVSASVFTLVAIAVERFRCIVYPFRQKLTLRKAIITIIIIWVLALIIMCPSAVTLTVTKDDFHFMVDDYNNYYPLYS--CWEEWPDT-EMRKIYTTVLFSHIYLAPLTLIVIMYARIAFKLFKSSAPIRGSVSEENEGRRVS----------KRKVRVIYMLIIVALFFSLSWLPLWTLMLLTDYGHLSDYQLN---LIAVYVFPFAHWLAFFNSSVNPIIYGYFNENFRRGFQEAFKVQVC 346
BLAST of GCR058 vs. Ensembl Xenopus
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:NCBI gene;Acc:100498122]) HSP 1 Score: 130.954 bits (328), Expect = 2.507e-33 Identity = 112/375 (29.87%), Postives = 185/375 (49.33%), Query Frame = 1
Query: 136 NQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKI--CLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQ-RVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXN---NQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLE 1230
N P IT + ++ H V + Y +IF+L ++GN V V+ MRTV N+FI NL+I+D+L +F +P TLL +I W FG+ +C+ S +QG +V AS+ TL IA DR+ I +P K+ + T +I++ IW+ + I+ P AV ++ G+ I C +WPN E ++ + V+F +Y+ PL+LI I YA +G+ +++ ++ +N + + R+K K++KML+ + + F +SWLPL+ ++L Y N NQ +N+ Y P L N+ INP IY N+ R + +C + + N S + + N E
Sbjct: 23 NYEEPNITYVDFYLHRPSVAAVFIASYLLIFILCMLGNGVVCFIVLSSKHMRTVTNLFILNLAISDLLVGIFCMPTTLLDNIIAGWPFGSTVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTAVVIIVVIWVLAIAIMCPSAVMSHVKEDKNFRVVIGNSNQTNPIYWCREDWPNP-EMRKIYTTVLFSNIYLAPLSLIVIMYARIGITLFKSELNVENKNHQ----QPRHAVSRRKQ-KVIKMLIIIALLFILSWLPLWTLMMLTDYANLTQNQYQMINI------YIYPFAHWLAFFNSSINPIIYGFFNENFRRGFQAAFKLQLCSEELERRTMYSHRAQGNAILPAASHNTARQNSAFE 385
BLAST of GCR058 vs. Ensembl Xenopus
Match: gamt (guanidinoacetate N-methyltransferase [Source:Xenbase;Acc:XB-GENE-947462]) HSP 1 Score: 129.413 bits (324), Expect = 9.440e-33 Identity = 114/372 (30.65%), Postives = 179/372 (48.12%), Query Frame = 1
Query: 124 EFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI-QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGV-IFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICC--RSIPGNGY-LQGASGYNVTFGTNSCSLRKANEG 1224
+F N+ T+ S V +L + Y +I V+++ GNI V VI++ M++ ++FI NL++ADI+ L P TL++ + + W FG L+C S ++Q +V S+ TL IA DR+ I HP+K ++ I +I IW ++ LP AVY K+ Q + N R +CL +P + +L + F++LYV+PL +ISI Y V K+W R + + + H R+K +KML+ VVV FA+ W PL L+L S N YF F S+ C NPFIY + + + A++C R P + LQ S T +C ++ +
Sbjct: 52 DFEND------TKYEAESQSSTVKALLIVAYSVIIVISLFGNILVCHVVIKNSRMQSATSLFIVNLAVADIMITLLNTPFTLVRFVSSTWVFGKLMCHVSRFVQYCSVHVSVLTLMAIALDRHQVIMHPLKPRMSTVKGIICIIVIWFMASGFSLPHAVYQKLEQFFIGNTV--------RMVCLPSFPYPADLFWKYLDLATFVLLYVLPLIVISITYTTVAKKLWLRNAIGDITMEQYYAH------RRKKKMTLKMLMLVVVVFAVCWFPLNCYLVLMSSKVISSNNA-------IYF--AFHWFAMSSTCYNPFIYCWLNESFRSELKALLCVCRRRSPAQSHPLQSVSPQFRHAWTENCHFKQGSSS 394
BLAST of GCR058 vs. Ensembl Xenopus
Match: taf1b (TATA-box binding protein associated factor, RNA polymerase I, B [Source:Xenbase;Acc:XB-GENE-992275]) HSP 1 Score: 126.716 bits (317), Expect = 3.867e-32 Identity = 103/333 (30.93%), Postives = 169/333 (50.75%), Query Frame = 1
Query: 190 VICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGA-SGYNVTF 1185
V IL Y +I +L ++GN V+ ++++ +MRTV N FI NL+IAD++ F LP TL+ ++ +EW FG++LC PY Q +V S TL +IA DRY I + I+K +++I+ WI++AI+ +P AV+ + + + F L +C W N+ + Y L ++ I+ Y +PL +I Y L++W ++ S T +++ KML+ +VV FA+ WLP +I L + W V + L Y IF V+ + +NPF+Y ++ Y G C+ N G+ G++ TF
Sbjct: 38 VQVILIAAYSLIILLGLVGNSLVIYMIVKYKNMRTVTNFFIANLAIADLMVDSFCLPFTLVYTLMDEWKFGSVLCHLFPYAQAMSVNVSTLTLIVIALDRYWCIVFHLNSRISKNLSFLIISITWITAAILAIPLAVFREFRYEDL-----PPFNLKIAVCAENWTNR-DSTIYSLSML-ILQYALPLAVICYAY----LRIWFKLKNHISP-------TTRSESQQRRKNTTKMLVMMVVVFAVCWLPFHIFQLAI----DLEWTVAIHENKLLY--TIFHVVAMCSTFVNPFLYGWMNKNYRHGFLTFFGCKDTLQNSQPDGSLRGHSYTF 346
BLAST of GCR058 vs. Ensembl Mouse
Match: Npffr2 (neuropeptide FF receptor 2 [Source:MGI Symbol;Acc:MGI:1860130]) HSP 1 Score: 145.591 bits (366), Expect = 1.066e-38 Identity = 111/344 (32.27%), Postives = 180/344 (52.33%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAV--------YYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGI-NAVICC--RSIPGNGY 1152
IT + Y+ H V + Y +IFVL ++GN V VIR+ M TV N FI NL+I+D+L +F +PITLL +I W FG+ +C+ S +QG +V AS+ TL IA DR+ + +P K + KT ++ ++ IW + I+ P A+ YY++++ N T + C +WP + E + + V+F +Y+ PL+LI I YA +G +++ + ++ H KK K++KMLLTV + F +SWLPL+ ++L Y + + N I+ Y P L N+ +NP IY ++ + +G +A C ++ P Y
Sbjct: 32 ITYVNYYLHQPQVAAVFISSYLLIFVLCMVGNTVVCFIVIRNRHMHTVTNFFILNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIAVDRFRCVVYPFKPKLTVKTAFVTIVIIWGLAIAIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYW------CREDWP-RHEMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTAAHCTGKQRPVQWHVS-----KKKQKVIKMLLTVALLFILSWLPLWTLMMLSDYTD---LSPNKLRIINIYIYPFAHWLAFCNSSVNPIIYGFFNENFRNGFQDAFQICQKKAKPQEAY 360
BLAST of GCR058 vs. Ensembl Mouse
Match: Qrfprl (pyroglutamylated RFamide peptide receptor like [Source:MGI Symbol;Acc:MGI:2441881]) HSP 1 Score: 119.398 bits (298), Expect = 2.588e-29 Identity = 108/386 (27.98%), Postives = 188/386 (48.70%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILS-----------ICYGM-IFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHP--MKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVW-QRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVIC---CRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSS 1257
+TR + H GL +L+ + GM IF L + GN V+ V R +MRTV NIFIC+L+++D+L F +P+T+LQ++ + W G +C+ P++Q T + I T+T IA +R+ + HP MKR + + ++ +W+ + II P ++++K L ++ CL EW + K Y I + L+++PL L+S+ Y +G ++W ++ D S + I RKK + V M++TVVV FA+ W P +I ++ Y N + + + I Q++G N+ NP IY + + + +C + P + G+SG V + R+ +EI +++ S
Sbjct: 19 LTRAQFIAHYGLRPLVLTPQLPARARLALLLVGMLIFALALFGNALVVYVVTRSKAMRTVTNIFICSLALSDLLIVFFCIPVTMLQNVSDTWLGGAFICKMVPFVQCTAIVTEILTMTCIAVERHQGLVHPFKMKRQYTNQRAFTMLGVVWLVAIIIGSPMWHVQRLEIKYDFL-----YEKEHICCLEEWSSPVHQKIY-TTFILVTLFLLPLLLLSVLYGKIGYELWIKKRIGDGSVLRTIHGKEMFKIARKKK-RAVIMMVTVVVLFAVCWAPFHIVHMMIEYSNFEK---EYDEVTIKMIFAIVQIIGFFNSICNPIIYALMNENFKKNFVSAVCYCIVKETPSSARKHGSSGAMVMHRRAKLAARE--NPVEIKGEAFGGS 392
BLAST of GCR058 vs. Ensembl Mouse
Match: Tacr2 (tachykinin receptor 2 [Source:MGI Symbol;Acc:MGI:98477]) HSP 1 Score: 117.857 bits (294), Expect = 5.743e-29 Identity = 92/322 (28.57%), Postives = 155/322 (48.14%), Query Frame = 1
Query: 172 FKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLG--VIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGIN-AVICC 1128
F G + + + Y + ++ + GN V+ ++ H MRTV N FI NL++AD+ A F + + HN W+FG+ C T + SI ++T IA DRY+AI HP + ++ +T ++ IW+ + + P Y I + G+ + C++ WPN GK L V+F+++Y +PL ++ Y+ +GL +W+R + L H + KK K VK ++ VVV FAI WLP ++ +L + + + ++ + L F L S+ NP IY + ++ G A CC
Sbjct: 26 FSMPGWQLALWATAYLALVLVAVTGNATVIWIILAHERMRTVTNYFIINLALADLCMAAFNATFNFIYASHNIWYFGSTFCYFQNLFPVTAMFVSIYSMTAIAADRYMAIVHPFQPRLSAPSTKAVIAVIWLVALALASPQCFYSTITVD------QGATK-----CVVAWPNDNGGKMLLLYHLVVFVLIYFLPLVVMFAAYSVIGLTLWKRAVPRHQAHGANLRH----LQAKK--KFVKAMVLVVVTFAICWLPYHLYFILGTFQEDIYYRKFIQQVYLALFW-----LAMSSTMYNPIIYCCLNHRFRSGFRLAFRCC 325
BLAST of GCR058 vs. Ensembl Mouse
Match: Npffr1 (neuropeptide FF receptor 1 [Source:MGI Symbol;Acc:MGI:2685082]) HSP 1 Score: 117.472 bits (293), Expect = 1.566e-28 Identity = 97/326 (29.75%), Postives = 164/326 (50.31%), Query Frame = 1
Query: 169 YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNL---TWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVIC 1125
Y++HS V + Y +IF+L ++GN V V+++ MRTV N+FI NL+++D+L +F +P TL+ ++ W F C+ S +QG +V AS+ TL IA +R+ I HP + + + + + IW + +I+ P AV + + + N S+ L+ C WP KG K Y V+F +Y+ PL LI + YA + K+ Q ++ ++ ++V ML+ V +FF +SWLPL++ LLL Y +++ ++ Y P+ L ++ NP IY N+ R + A +C
Sbjct: 35 YYQHSSPVAAMFIAAYALIFLLCMVGNTLVCFIVLKNRHMRTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALLTIAVIWALALLIMCPSAVTLTVTREEHHFMLDARNRSYPLYS--CWEAWPEKGMRKVY-TAVLFAHIYLAPLALIVVMYARIARKLCQAPGPARDAEEAVAEGG---RASRRRARVVHMLVMVALFFTLSWLPLWVLLLLIDYGELSELQLHLLSV---YAFPLAHWLAFFHSSANPIIYGYFNENFRRGFQAAFRAQLC 351
BLAST of GCR058 vs. Ensembl Mouse
Match: Qrfpr (pyroglutamylated RFamide peptide receptor [Source:MGI Symbol;Acc:MGI:2677633]) HSP 1 Score: 108.612 bits (270), Expect = 1.875e-25 Identity = 92/328 (28.05%), Postives = 165/328 (50.30%), Query Frame = 1
Query: 202 LSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVW--QRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVIC---CRSIPGNGYLQGASG 1170
++ +IF L + GN V+ V R +MRTV NIFIC+L+++D+L A F +P+T+LQ+I ++W G +C+ P++Q T V I T+T IA +R+ + HP K T I + A I++ +++ ++++ ++ ++ CL EW + + Y I +IL+++PL ++ + Y+ +G ++W +RV D+S + I RKK + V M++TVV FA W P ++ ++ Y N + + + + Q +G N+ NPF+Y + + + +C R G G SG
Sbjct: 48 FALAGALIFALALFGNSLVIYVVTRSKAMRTVTNIFICSLALSDLLIAFFCIPVTMLQNISDKWLGGAFICKMVPFVQSTAVVTEILTMTCIAVERHQGLIHPFKMKWQYTTRRAFTILGVVWLAAIIVGSPMWHVQRLEI---KYDFLYEKEHVCCLEEWASPMHQRIY-TTFILVILFLLPLVVMLVLYSKIGYELWIKKRVG-DSSALQTIHGKEMSKIARKKK-RAVVMMVTVVALFAACWAPFHVVHMMVEYSN---FEKEYDDVTIKMVFAVAQTIGFFNSICNPFVYAFMNENFKKNFLSAVCYCIVRETFSPGQKPGNSG 366
BLAST of GCR058 vs. UniProt/SwissProt
Match: sp|Q8IN35|SIFAR_DROME (Neuropeptide SIFamide receptor OS=Drosophila melanogaster OX=7227 GN=SIFaR PE=2 SV=2) HSP 1 Score: 162.54 bits (410), Expect = 2.717e-42 Identity = 110/325 (33.85%), Postives = 190/325 (58.46%), Query Frame = 1
Query: 166 PYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI--QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRS 1134
P ++HS + + + Y ++F++ +IGN V+ V+R MRTV N FI NL+IADIL +F LP TL+ +I W G L+C+ PY+QG +V AS+ +L ++ DR++AI P+K+ + K+ I++IGIW+ + + +PW +++ + +V + ++ + +C WP +G YFL + Y++P++LI++ Y + +KV R S K ++ + +K +K++KML+ VV+ F +SWLPLY+ + ++ S + +L +P+ Q LG+SN+CINP I Y+ ++KY G A+I RS
Sbjct: 199 PLYRHSLAMSMVYCVAYIVVFLVGLIGNSFVIAVVLRAPRMRTVTNYFIVNLAIADILVIVFCLPATLIGNIFVPWMLGWLMCKFVPYIQGVSVAASVYSLIAVSLDRFIAIWWPLKQ-MTKRRARIMIIGIWVIALVTTIPWLLFFDLVPAEEVFSDALVSAYSQPQFLCQEVWPPGTDGNLYFLLANLVACYLLPMSLITLCYVLIWIKVSTR-SIPGESKDAQMDR----MQQKSKVKVIKMLVAVVILFVLSWLPLYVIFARIKFGSDISQE---EFEILKKVMPVAQWLGSSNSCINP-ILYSVNKKYRRGFAAIIKSRS 513
BLAST of GCR058 vs. UniProt/SwissProt
Match: sp|Q9Y5X5|NPFF2_HUMAN (Neuropeptide FF receptor 2 OS=Homo sapiens OX=9606 GN=NPFFR2 PE=1 SV=2) HSP 1 Score: 154.451 bits (389), Expect = 2.498e-40 Identity = 117/357 (32.77%), Postives = 188/357 (52.66%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAV--------YYKIQMKVTNLT----WNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICC-RSIPGNGYLQGASGY 1173
IT + Y+ H V I I Y +IF L ++GN V V+R+ M TV N+FI NL+I+D+L +F +PITLL +I W FG +C+ S +QG +V AS+ TL IA DR+ + +P K + KT ++I++ IW+ + I+ P AV YY++++ N T W C +WPN+ E ++ + V+F +Y+ PL+LI I Y +G+ ++ R + ++G+K N + +K K++KMLL V + F +SWLPL+ ++L Y + + N I+ Y P L N+ +NP IY N+ R + + +C R+ P Y A +
Sbjct: 134 ITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQNKTSPVYW----------CREDWPNQ-EMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLF-RAAVPHTGRK---NQEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYAD---LSPNELQIINIYIYPFAHWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSH 472
BLAST of GCR058 vs. UniProt/SwissProt
Match: sp|Q9EQD2|NPFF2_RAT (Neuropeptide FF receptor 2 OS=Rattus norvegicus OX=10116 GN=Npffr2 PE=2 SV=1) HSP 1 Score: 146.362 bits (368), Expect = 3.927e-38 Identity = 109/344 (31.69%), Postives = 178/344 (51.74%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAV--------YYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAV--ICCRSI-PGNGY 1152
IT + Y+ H V + Y +IF L ++GN V VIR+ M TV N FI NL+I+D+L +F +PITLL +I W FG+ +C+ S +QG +V AS+ TL IA DR+ + +P K + KT +++++ IW + I+ P A+ YY++++ N T + C +WPN+ E + + V+F +Y+ PL+LI I YA +G +++ + ++ H KK K++KMLLTV + F +SWLPL+ ++L Y + + N ++ Y P L N+ +NP IY ++ + G C + + P Y
Sbjct: 32 ITYMNYYLHQPHVTAVFISSYFLIFFLCMVGNTVVCFVVIRNRYMHTVTNFFIFNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIAVDRFRCVVYPFKPKLTVKTAFVMIVIIWGLAITIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYW------CREDWPNQ-EMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTSAHSTGKQRLEQWHVS-----KKKQKVIKMLLTVALLFILSWLPLWTLMMLSDYAD---LSPNKLRVINIYVYPFAHWLAFCNSSVNPIIYGFFNENFRSGFQDAFQFCQKKVKPQEAY 360
BLAST of GCR058 vs. UniProt/SwissProt
Match: sp|Q924H0|NPFF2_MOUSE (Neuropeptide FF receptor 2 OS=Mus musculus OX=10090 GN=Npffr2 PE=2 SV=2) HSP 1 Score: 145.591 bits (366), Expect = 7.457e-38 Identity = 111/344 (32.27%), Postives = 180/344 (52.33%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAV--------YYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGI-NAVICC--RSIPGNGY 1152
IT + Y+ H V + Y +IFVL ++GN V VIR+ M TV N FI NL+I+D+L +F +PITLL +I W FG+ +C+ S +QG +V AS+ TL IA DR+ + +P K + KT ++ ++ IW + I+ P A+ YY++++ N T + C +WP + E + + V+F +Y+ PL+LI I YA +G +++ + ++ H KK K++KMLLTV + F +SWLPL+ ++L Y + + N I+ Y P L N+ +NP IY ++ + +G +A C ++ P Y
Sbjct: 32 ITYVNYYLHQPQVAAVFISSYLLIFVLCMVGNTVVCFIVIRNRHMHTVTNFFILNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIAVDRFRCVVYPFKPKLTVKTAFVTIVIIWGLAIAIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYW------CREDWP-RHEMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTAAHCTGKQRPVQWHVS-----KKKQKVIKMLLTVALLFILSWLPLWTLMMLSDYTD---LSPNKLRIINIYIYPFAHWLAFCNSSVNPIIYGFFNENFRNGFQDAFQICQKKAKPQEAY 360
BLAST of GCR058 vs. UniProt/SwissProt
Match: sp|Q96P65|QRFPR_HUMAN (Pyroglutamylated RF-amide peptide receptor OS=Homo sapiens OX=9606 GN=QRFPR PE=1 SV=2) HSP 1 Score: 133.65 bits (335), Expect = 2.101e-33 Identity = 95/348 (27.30%), Postives = 168/348 (48.28%), Query Frame = 1
Query: 220 MIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVW--QRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSS 1257
+IF L + GN V V R +MRTV NIFIC+L+++D+L F +P+T+LQ+I + W G +C+ P++Q T V I T+T IA +R+ + HP K + + +++ +++ Q+++ ++ ++ CL EW + K Y I +IL+++PL ++ I Y+ +G ++W +RV D S + I RKK + V M++TVV FA+ W P ++ ++ Y N + + + I Q++G SN+ NP +Y + + + + +C Y + T S + R N G+ + K K S
Sbjct: 54 LIFALALFGNALVFYVVTRSKAMRTVTNIFICSLALSDLLITFFCIPVTMLQNISDNWLGGAFICKMVPFVQSTAVVTEILTMTCIAVERHQGLVHPFKMKWQYTNRRAFTMLGVVWLVAVIVGSPMWHVQQLEI---KYDFLYEKEHICCLEEWTSPVHQKIY-TTFILVILFLLPLMVMLILYSKIGYELWIKKRVG-DGSVLRTIHGKEMSKIARKKK-RAVIMMVTVVALFAVCWAPFHVVHMMIEYSNFEK---EYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVLSAVC---------------YCIVNKTFSPAQRHGNSGITMMRKKAKFS 377
BLAST of GCR058 vs. TrEMBL
Match: A0A193KU97 (GCR058 OS=Schmidtea mediterranea OX=79327 GN=gcr058 PE=2 SV=1) HSP 1 Score: 731.865 bits (1888), Expect = 0.000e+0 Identity = 389/391 (99.49%), Postives = 391/391 (100.00%), Query Frame = 1
Query: 121 MEFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ 1293
MEFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYIIVIGIWISSAIILLPWAVYYKIQ+KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRV+TDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWLPLYITLLLYYYLNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ
Sbjct: 1 MEFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYIIVIGIWISSAIILLPWAVYYKIQIKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVNTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWLPLYITLLLYYYLNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ 391
BLAST of GCR058 vs. TrEMBL
Match: A0A193KUW2 (GCR402 OS=Schmidtea mediterranea OX=79327 GN=gcr402 PE=2 SV=1) HSP 1 Score: 287.345 bits (734), Expect = 2.204e-90 Identity = 161/331 (48.64%), Postives = 221/331 (66.77%), Query Frame = 1
Query: 172 FKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKI-CLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRV---STDNSGKKFCL-NHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNG 1149
F++S I IL + YG+I ++ IIGN+ V+ TV+ H M+TVINI ICNLS+AD+LCA+FVLP+ LQSI EW +G +LCR++PYLQG +VC SI TLT++AFDRYVAICHP+ R++N I+ IWI SAI+LLPWA+YY+ +M H KI C+ WP+ + ++YF+ VIFI+ Y+IPL LIS FY A+ ++++R S + K+ L + GGI R KSI++VKML VVV FA+SWLPLYI + YY + W+ ++ Y +PIFQ++ ASN+CINP+IY YS+ Y DG VI C+ N
Sbjct: 9 FRYSIAFITILGVSYGLILLMAIIGNLIVITTVLWHPCMKTVINILICNLSVADLLCAIFVLPMNFLQSITKEWLYGEILCRSTPYLQGVSVCTSIMTLTVVAFDRYVAICHPLHRILNISKATSIISMIWILSAILLLPWALYYRTEMD----------NNHTKIVCVQAWPDNADERDYFIFVIFIMCYIIPLFLISCFYFAIASRIYKRANANSNETQSKRIPLIKLSSGGISRTKSIRIVKMLAIVVVTFAVSWLPLYIVFIFLYYSDKNKWSPQFLNVLFDYLIPIFQLISASNSCINPWIYCLYSQTYRDGFRNVIICKKATLND 329
BLAST of GCR058 vs. TrEMBL
Match: A0A210QEM7 (Neuropeptide FF receptor 2 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT05355 PE=3 SV=1) HSP 1 Score: 206.453 bits (524), Expect = 1.812e-58 Identity = 126/333 (37.84%), Postives = 197/333 (59.16%), Query Frame = 1
Query: 130 NNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICC 1128
NN + + P +H+ +I + ++ Y ++F+ GN+ V++ + R+ + TV N FI NL++ADIL A+F +PITLL +I+ W +G+++C+ +PYLQG +VCAS++TL IA DRY+AIC+ + + K +++ +W + I++PWAVYY+ + S Q IC + WP++ GK +FL IF++ Y IPL LI + YA +GL+VW R N+ F N GI K +K++KMLL VV+ F +SWLPLY L + ++N TI+ Y +P+ Q LG +N+ INP IY +SRK I ++ C
Sbjct: 34 NNTERY-----FPVLQHATPIIVVYTMAYLLVFLFAFFGNLIVVIVIWRNRWLHTVTNFFIVNLAVADILVAIFCVPITLLTNIYTGWPYGSMMCKVTPYLQGVSVCASVNTLAAIALDRYLAICYTLNYKLTGKIARVVMGCVWAFALCIMIPWAVYYQHDQ------YRSSIQ-AIYICRLVWPSQVSGK-FFLAGIFLVCYTIPLLLIVVCYALIGLRVWNR----NAPGIFKSN----GIIHKSKVKVIKMLLVVVILFGLSWLPLYAVNLKIVFSPPSLTDMNTVTILYDYLVPVSQWLGLANSGINPLIYCLFSRKIRTRIKVMVTC 345
BLAST of GCR058 vs. TrEMBL
Match: A0A3S1HNE0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Elysia chlorotica OX=188477 GN=EGW08_009325 PE=3 SV=1) HSP 1 Score: 204.527 bits (519), Expect = 5.021e-57 Identity = 132/350 (37.71%), Postives = 211/350 (60.29%), Query Frame = 1
Query: 94 CFGKT*LFLMEFNNNQS-FPIITRLPY--FKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRS 1134
C G +F + N+ S P +T Y K ++ ILS+ YG++F+L ++GNI+V++ V R S+ T N F+ N+++AD+L A+F LP+TLL +I+ W FG ++C+A PYLQG VCAS+ TL +IA DRY+AICH M++ K+ I+I IW+ + ++ PWA++YK +L + + Q+ ICL ++P+ + + +FL VIF++ Y +PLT I + Y+ +G +V R + + K G+ +K I++VKML+ VVV FA SW+PLYI L + S + + ++ +P Q LG SN+C+NP +Y +S+K I A++CC S
Sbjct: 63 CTGLENMFTGQGNSTASPEPAVTEEYYKLVKQPVFMLVILSVSYGIVFLLALLGNISVIIVVARDKSLHTATNFFLVNMAVADMLVAIFCLPLTLLSNIYTGWQFGAIMCKAVPYLQGVAVCASVCTLVVIAVDRYIAICHVMRQRNTKRMARTILIIIWLLAMTLMTPWALFYK------HLEYRTTLQII-YICLPDFPDVTQSRIFFLAVIFLVCYALPLTFIVVCYSMIGYRVCHRNAPGITSSK--------GVIQKSKIRVVKMLVVVVVLFAFSWMPLYIINLKMNFFPPPSRSAEMD-VIQKVLIPFAQWLGTSNSCMNPLVYCLFSQKIRRRIKALLCCSS 396
BLAST of GCR058 vs. TrEMBL
Match: A0A2C9JTE5 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106067534 PE=3 SV=1) HSP 1 Score: 199.134 bits (505), Expect = 1.849e-55 Identity = 145/369 (39.30%), Postives = 215/369 (58.27%), Query Frame = 1
Query: 136 NQSFPI--ITRLP-YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWX-XXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLE 1230
N SF I +TR Y K S ++ +LSICYG F+L I+GNI+VL + ++ + IFI NL++AD+ A+ LPIT + +I+ W FG LC A PYLQG +V AS++TL +IA DRY+AICH M++ KK +I++ IW S +ILLPWA+YY + +L +G + +C +P++ K +FLGVIF++LY IPLTLI++FY +G +VW R + + K G+ K +K+VKML+ VV+ F ISW+ I L ++++ N+S I+ TY P Q LG SN+C+NP +Y +S+K I +++CC G S YN S S R ++ L+
Sbjct: 31 NTSFNITNVTRAENYVKQSTFMVVMLSICYGGAFLLAIVGNISVLCIIFKNRRFHSATYIFIGNLAVADLCMAVICLPITYVTNIYAYWMFGPFLCTAIPYLQGVSVSASVNTLVVIALDRYIAICHVMRQRNTKKNARVILVIIWTFSLLILLPWALYY----RQYDLLIDG---VMYTVCNEIFPSEEAKKGFFLGVIFLLLYCIPLTLIAVFYFLIGYRVWNRDAPGITSSK--------GVIEKSKMKVVKMLMVVVLLFMISWMPLYVIRLNVFFFGGNES-----DPIINTYVNPFAQWLGTSNSCMNPLVYCLFSQKIRRRIWSMLCCAKKEARGLYSTRSMYNSAL-QQSVSTRDSSIRLD 378
BLAST of GCR058 vs. Ensembl Cavefish
Match: npffr1l3 (neuropeptide FF receptor 1-like [Source:NCBI gene;Acc:111195007]) HSP 1 Score: 149.058 bits (375), Expect = 1.095e-39 Identity = 114/373 (30.56%), Postives = 191/373 (51.21%), Query Frame = 1
Query: 166 PYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLT-WNGSFQLHRKI--CLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICCRSIPGN--GYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSS 1257
PY++HS + + + Y IF+L +IGN V L V+R+ M TV N FI NLSI+D+L +F +P TL+ ++ W F ++C+ S +QGT+VCAS+ TL IA DR+ I +P K + + IW+ + +I+ P A+ ++ + + T +NG + L + C WP+ + ++ + V+F +Y++PL LI + Y +G K++ N + H + ++K IK++KML+ V + F +SWLPL+ +LL Y + + + T Y P L +N+ +NP IY N+ R +H CC + G + G V+ + +L N L + N+ Y S
Sbjct: 49 PYYQHSLGMAAVFILAYLFIFLLCMIGNGVVCLIVLRNRHMWTVTNAFILNLSISDLLVGIFCIPTTLVDNLITGWPFSDVMCKLSGLVQGTSVCASVFTLVAIAVDRFRCIVYPFKPKLTLLVAKATIGLIWLLALVIMFPSALMLTVEQERGHFTVYNGDYNLSYPLYSCYETWPDP-QMRKVYTTVLFAHIYLVPLVLIILMYGRIGAKLYTTTVLVNVEQADGTPHGKCPVSQRK-IKVIKMLIVVALLFMLSWLPLWTLMLLTDYARPEGEQLELLT---GYVFPFSHWLAFANSSVNPIIYGYFNENFKRGFHAVCQQTSCCSRGRHSRLGIKRPERGAEVSAHLDK-TLNSNNLSLGVRNRVYTDS 415
BLAST of GCR058 vs. Ensembl Cavefish
Match: npffr2b (neuropeptide FF receptor 2-like [Source:NCBI gene;Acc:103031865]) HSP 1 Score: 145.976 bits (367), Expect = 1.327e-38 Identity = 110/327 (33.64%), Postives = 178/327 (54.43%), Query Frame = 1
Query: 148 PIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKI--CLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQ-RVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAV 1119
P IT + ++ H V I + Y +IFV+ ++GN V V+R SMRTV N+FI NL+I+D+L +F +P TL+ +I W FG+L+C+ S +QG +V AS+ TL +IA DR+ I +P K+ + T+ +I++ IW+ + I+ P V ++ + G R C WP++ E ++ + V+F +Y+ PLTLI I YA +G+ +++ V G HT + RKK +++KMLL V + F +SWLPL+ ++L Y + + V I Y P+ L N+ +NP IY ++ + G V
Sbjct: 33 PNITYVDFYLHEPSVSAIFIVSYLLIFVVCMVGNGLVCFIVLRSKSMRTVTNLFILNLAISDLLVGIFCMPTTLVDNIITGWPFGSLVCKLSGMVQGISVSASVFTLVVIAVDRFRCIVYPFKQKLTISTSTVIIVIIWVLAISIMCPSGVMLQVTKESRVSILLGESNATRPFYWCRENWPSQ-EMRKIYTTVLFANIYLAPLTLIVIMYARIGITLFKTAVPVPTRGGYEGRGHT---VSRKKK-RVIKMLLVVALLFILSWLPLWTLMMLSDYASLSEYQHRVINI---YVYPLAHWLAFCNSSVNPIIYGFFNENFRRGFQIV 351
BLAST of GCR058 vs. Ensembl Cavefish
Match: npffr1 (neuropeptide FF receptor 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8106]) HSP 1 Score: 144.436 bits (363), Expect = 1.932e-38 Identity = 109/341 (31.96%), Postives = 179/341 (52.49%), Query Frame = 1
Query: 169 YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTW---NGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAV----ICCRSIPGNGYLQGASG 1170
Y++HS V + + Y +IF L ++GN+ V L V+++ MRTV NIFI NL+I+D+L +F LPITL ++ W F + C+ S +QG +V AS+ TL IA +R+ I +P ++ + + I + IW + II+ P AV + + N ++ L+ C WP++ K Y + V+F +Y+ P+TLI I Y + LK+++ ++ + G ++ I++V MLL V + F +SWLPL+I +LL Y N S +NV + Y P L N+ INP +Y ++ + G A +C +P + A+G
Sbjct: 37 YYQHSPPVATVFILAYILIFFLCMVGNLLVCLIVLKNKQMRTVTNIFILNLAISDLLVGIFCLPITLADNLVTGWPFDEVTCKMSGLIQGASVSASVFTLVAIAVERFRCIVYPFQQKLTLRQAIITIAFIWALAIIIMCPSAVTLTVSHDAFHFMVDRDNATYPLY--TCWEAWPDQSMRKIYTM-VLFSHIYLAPVTLIVIMYTRIALKLFKSPASIRDAQA----EDEGRRVYRRKIRVVNMLLIVALLFTVSWLPLWIIILLTDYGNMSSSQLNV---IAVYIFPFAHWLAFFNSSINPIVYGYFNENFRRGFQAAFKFGLCLVEMPPQPTVAMAAG 367
BLAST of GCR058 vs. Ensembl Cavefish
Match: npffr1 (neuropeptide FF receptor 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8106]) HSP 1 Score: 141.739 bits (356), Expect = 1.248e-37 Identity = 111/341 (32.55%), Postives = 181/341 (53.08%), Query Frame = 1
Query: 169 YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTW---NGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAV----ICCRSIPGNGYLQGASG 1170
Y++HS V + + Y +IF L ++GN+ V L V+++ MRTV NIFI NL+I+D+L +F LPITL ++ W F + C+ S +QG +V AS+ TL IA +R+ I +P ++ + + I + IW + II+ P AV + + N ++ L+ C WP++ K Y + V+F +Y+ P+TLI I Y + LK+++ + G++ + R+K I++V MLL V + F +SWLPL+I +LL Y N S +NV + Y P L N+ INP +Y ++ + G A +C +P + A+G
Sbjct: 27 YYQHSPPVATVFILAYILIFFLCMVGNLLVCLIVLKNKQMRTVTNIFILNLAISDLLVGIFCLPITLADNLVTGWPFDEVTCKMSGLIQGASVSASVFTLVAIAVERFRCIVYPFQQKLTLRQAIITIAFIWALAIIIMCPSAVTLTVSHDAFHFMVDRDNATYPLY--TCWEAWPDQSMRKIYTM-VLFSHIYLAPVTLIVIMYTRIALKLFK-----SPGRR---------VYRRK-IRVVNMLLIVALLFTVSWLPLWIIILLTDYGNMSSSQLNV---IAVYIFPFAHWLAFFNSSINPIVYGYFNENFRRGFQAAFKFGLCLVEMPPQPTVAMAAG 346
BLAST of GCR058 vs. Ensembl Cavefish
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:NCBI gene;Acc:103039748]) HSP 1 Score: 140.198 bits (352), Expect = 9.343e-37 Identity = 105/348 (30.17%), Postives = 179/348 (51.44%), Query Frame = 1
Query: 154 ITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKI--QMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLN-HTCGGIDRKKSIKLVKMLLTVVVFFAISWX---XXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNV 1179
IT + ++ H V I + Y +IF++ ++GN V V+R +MRTV N+FI NL+++D+L +F +P TLL +I W FG+++C+ S +QG +V AS+ TL IA DR+ I +P K+ + T +I++ IW+ + I+ P V ++ + + +G+ C WPN+ E ++ + V+F +Y+ PL+LI I YA +G+ ++ + + GK N HT KK +++KMLL V + F +SWL L + +Q +N+ Y P L N+ +NP IY ++ + G A+ R G+ + + V
Sbjct: 33 ITYVGFYLHQPSVAAIFIVSYLLIFLVCMVGNGVVCFIVLRSKNMRTVTNLFILNLAVSDLLVGIFCMPTTLLDNIITGWPFGSMVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATLIIVIIWVLAISIMCPSGVMLQVTKEQSIRVFLGDGNRTSPFYWCRENWPNQ-EMRKIYTTVLFANIYLAPLSLIVIMYARIGITLF-KTAVPTGGKPGNDNRHTVS----KKKQRVIKMLLIVALLFILSWLPLWTLMMLTDYARLTEHQYRLINI------YIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAIFKFRLCTAGGHRRKTYSHRV 368
BLAST of GCR058 vs. Ensembl Sea Lamprey
Match: npffr1l3 (neuropeptide FF receptor 1 like 3 [Source:ZFIN;Acc:ZDB-GENE-070424-44]) HSP 1 Score: 133.265 bits (334), Expect = 1.958e-35 Identity = 98/326 (30.06%), Postives = 168/326 (51.53%), Query Frame = 1
Query: 169 YFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMK-----VTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKV-----WQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINA 1116
+++H+ + I Y IF+L +IGN V V++ MRTV N+FI NL+++D+L +F +PITL+ ++ W FG ++C+ S +QGT+V AS+ TL IA DR+ I +P KR + ++ IW + I+ P A+ ++ V + N + LH+ C WP+ K Y V+F +Y+ PL LI + YA +G ++ + + G+ R++ +K++KML+ V + F +SWLPL+ ++L Y N W++ + Y P+ L N+ +NP +Y ++ + G A
Sbjct: 41 FYQHAPPTAAVYLIAYLFIFLLCMIGNGLVCYVVLQSRRMRTVTNLFILNLAVSDLLVGIFCMPITLVDNLITGWPFGVVVCKMSGLVQGTSVSASVFTLVAIAVDRFRCIVYPFKRKLRISHALFTILVIWALALSIMCPSALMLTAEVSRGHYMVYSDGSNDTHPLHQ--CWEAWPDPRMRKAY-TTVLFANVYLAPLALILLMYARIGFRLCSLSASAAAAASAAAPSRGGGGGATGVTRRR-VKVIKMLIIVAMLFMLSWLPLWTVMMLVDYANLSPWDLAFLNM---YVYPVAHWLAFFNSSVNPIVYGYFNENFRRGFQA 359
BLAST of GCR058 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008040.1 (pep scaffold:Pmarinus_7.0:GL477351:476361:492361:-1 gene:ENSPMAG00000007251.1 transcript:ENSPMAT00000008040.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 123.635 bits (309), Expect = 9.359e-32 Identity = 70/223 (31.39%), Postives = 120/223 (53.81%), Query Frame = 1
Query: 172 FKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLG--VIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKF 834
F + + S+ YG I + GN+ V+ V+ H MRTV N F+ NL++AD A I + ++HN+W+FG CR ++ V ASI ++T IA DRY+AI HP+K + ++T +++ GIW + + LP ++Y +++ + R +CL++WP K G F+ V+ ++YV PL ++++ Y VG+ +W +S + F
Sbjct: 50 FAQRPWQVALWSVAYGGIVAVAFFGNMVVIWIVVAHRRMRTVTNYFLVNLAVADSAMAALNTSINFVYAVHNDWYFGGAYCRFQNFIPVAAVFASIYSMTAIAVDRYMAIRHPLKPRMTAQSTRLVMAGIWALAMCLSLPLSIYSRVEQR-----------FGRFVCLVDWPGKMGGDSQFIYQLVVMTLVYVFPLLVMAVTYTIVGVTLWGGTIPGDSSEHF 261
BLAST of GCR058 vs. Ensembl Sea Lamprey
Match: qrfpr4 (pyroglutamylated RFamide peptide receptor 4 [Source:ZFIN;Acc:ZDB-GENE-091204-354]) HSP 1 Score: 117.857 bits (294), Expect = 5.364e-30 Identity = 80/341 (23.46%), Postives = 164/341 (48.09%), Query Frame = 1
Query: 124 EFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXN-NQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIY----YNYSRKYHDGINAVICCR 1131
EF P + +P+ + ++ ++F L + GN V+ V R ++ TV N+F+C+L+++D++ +F +PITL+Q+ + W G LC+ P++Q T V S+ T+ IA +R+ I HP++ T + + + + ++Y ++V T++ + + C +W + E ++ + + ++ +V+PL ++ Y + ++W + ++ I R K + VKM++ VV+ FA+ W P ++ L N S+++ T L + Q+ G SN+ NP +Y NY ++ ++ ++C R
Sbjct: 24 EFIRAYHLPPMVYVPHLPQP--LRLAFAVVGALVFTLALSGNALVIFVVARRKALHTVANVFVCSLAVSDLMITVFCIPITLMQNFFSNWIAGVALCKLVPFIQVTAVTTSMLTMACIAVERFQGILHPLRSRSRYTTARAASMLCGVWLVGLCVAAPMFYAHTVQV---TYDVLYDVSHTGCSEQW-SHAEHRQAYTTTLLLVTFVLPLLTMTALYTRLAHELWVKTRVHDAVFNALQRSEINKITRSKK-RAVKMMVIVVILFAVCWAPFHVVNSLVPKGNLEMSFDMEAIT------LAMVQLFGFSNSLWNPIVYAALNQNYKKQLAIALSCLMCRR 351
BLAST of GCR058 vs. Ensembl Sea Lamprey
Match: sstr1a (somatostatin receptor 1a [Source:ZFIN;Acc:ZDB-GENE-090429-1]) HSP 1 Score: 96.6709 bits (239), Expect = 1.840e-22 Identity = 87/323 (26.93%), Postives = 151/323 (46.75%), Query Frame = 1
Query: 214 YGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXX--XXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVV-FFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCR-------SIPGNGY 1152
Y ++ ++ + GN V+ ++R+ M+T NI+I NL++AD L + V P + + W FG+ LCR + G + S+ LT++ FDRYVA+ HP+K ++ + I S +I+LP VY T + +G+ +C + WP + E F+ F + +++P+ I + YA + K+ R +G + RK+S + V +++T+VV F W+P Y+ L+ + Q VN ++L Y +N+C NP +Y S + V+C R PG+ Y
Sbjct: 20 YSLVCLVGLFGNSMVIYVILRYAKMKTATNIYILNLAVADELFMMSV-PFLATSTALHHWPFGSSLCRLVLSVDGINMFTSVFCLTVLTFDRYVAVVHPIKAARYRRPSVAKTINAAVWALSMVIILPIVVYAD-----TRVNADGTV-----VCNLLWP-RPEWSAAFVVYTFTVGFLLPVAAICLCYALIVAKM--RAVALRAGWQ----------QRKRSERKVTLMVTMVVAVFVACWMPFYVVQLVNVFEGQQGATVNQLAVILGY----------ANSCANPILYGFLSDSFRHSFQRVLCVRWAERYGADDPGDYY 308
BLAST of GCR058 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010661.1 (pep scaffold:Pmarinus_7.0:GL479584:296:6514:-1 gene:ENSPMAG00000009652.1 transcript:ENSPMAT00000010661.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 93.5893 bits (231), Expect = 7.921e-22 Identity = 65/206 (31.55%), Postives = 104/206 (50.49%), Query Frame = 1
Query: 214 YGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNL---TWNGSFQLHRKICL-MEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDN 819
YG+ VL + GN V+ + RT I+ F+ NL++AD+ ALF LP T + + W FG LLC + Q +V SI TLT I DRY A+CHP+ + K +++ L WAV I + T L T QL + CL + WP++G + Y + ++ + L ++++ Y++V +++W R + N
Sbjct: 6 YGLATVLALSGNAVVVWVLALGRRARTDISAFLINLALADLTMALFCLPFTFTEVLLQSWLFGALLCPLVRFAQTLSVSVSIYTLTTIGIDRYYAVCHPLHSRMTKSRGKVLIA----------LIWAVSISISLSTTTLAVATRGPMLQLGKGKCLEVGWPSRGYQQSYTVALLLGTYLLP-LLVLTLAYSSVAVRLWGRRTPGN 200
BLAST of GCR058 vs. Ensembl Nematostella
Match: EDO44418 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVN5]) HSP 1 Score: 124.79 bits (312), Expect = 8.103e-33 Identity = 90/303 (29.70%), Postives = 155/303 (51.16%), Query Frame = 1
Query: 208 ICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGK---EYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDG 1107
+ Y I L+++GN V+ TV R+ M++V N+FI NL+ +D+L +P + + EW FGT+LC+ YLQ +V +S+ TL I DR+VAI P + ++ KT II+L W++ I + T ++H C +W + K + V+F++LY +P+ +++ YA + K+W+R G+ + + RK K++KML+TV++ F SWLPL++ + ++ N YF+ +F LG N+ NP++Y +++ Y DG
Sbjct: 1 LAYATIIALSLVGNTVVIFTVCRNRHMKSVTNLFIANLAASDLLITFLGMPHQITELYTTEWMFGTVLCKLVVYLQSVSVGSSVLTLLAITKDRFVAIIFPFRPRMSFKTA----------KTIIMLIWSLSLGIMAPLIYAT-----KVHNNQCTEDWTPAFDAKTAPRDYTVVLFVMLYAVPMLTMAVLYAIIVRKLWRR---RRIGEVIPSASSKNEVSRK---KVIKMLITVIMLFTFSWLPLHVFSFISFFAPFCPDTSNTA----LYFVSLF--LGHGNSAANPYLYALFNQNYRDG 276
BLAST of GCR058 vs. Ensembl Nematostella
Match: EDO49570 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGN9]) HSP 1 Score: 114.005 bits (284), Expect = 7.109e-29 Identity = 82/305 (26.89%), Postives = 147/305 (48.20%), Query Frame = 1
Query: 211 CYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMK-RVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWP---NKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGIN 1113
Y +I VL+++GN V+ V+ H + R++ + F+ NL+ +DIL +P + + W FG ++C+ YLQ +V +S+ TL + DR+++I P++ R+ K YI+ + W + I + + F C+ EW N E + F ++F+++YV PL +++ Y A+ +W+R S G + T RK +++KMLL V + FA+ W + + Q++ K YF+ +F LG SN+ INPF+Y ++ + +G
Sbjct: 2 AYVLIIVLSLVGNSTVIYVVLNHRA-RSMTDTFVANLAASDILITFTGMPNMITEQYIRHWVFGPIVCKLVVYLQSVSVASSVLTLLAVTIDRFISIVFPLRGRIALSKAKYIVAVT-----------WIISLSIMAPLIDAQKAKQFPDGMYYCIEEWQPPFNPLEAPKSFTVLLFVVMYVAPLLTMAVLYCAICRTLWKRTS---PGARITRTETHLQRSRK---RVLKMLLAVTLLFAVCW-LPLWIFQFIMFFSPQTFPCFSKPF---YFVSLF--LGHSNSAINPFLYSFFNANFREGFR 282
BLAST of GCR058 vs. Ensembl Nematostella
Match: EDO50021 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF31]) HSP 1 Score: 109.768 bits (273), Expect = 2.291e-27 Identity = 93/298 (31.21%), Postives = 156/298 (52.35%), Query Frame = 1
Query: 208 ICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYH 1101
I Y I ++ +GN+ V+L R MR V N FI NL++AD + +PI+L+ + W F + LC+ PYLQG TV AS+ TL IA DR++ I P KR T+I V+ IW+ SA + P +Y ++ ++ + L+ C+ WP++ +G++ + ++F++L+++P+T IS+ YAA+ K+ H + + KLV+ML+ V+V FA+ W+P ++T L + + T + + Q L +N+C+NP IY Y+ Y
Sbjct: 1 ISYIFICIIGALGNLLVMLAT-RKRRMRNVTNQFIANLALADFTVCVITIPISLVYTNLGHWPFRSWLCKTMPYLQGITVSASVGTLVAIASDRFIVIVFPTKRKFRISRTWITVVAIWLLSAAVAFPVHLYSEVTREMGD-------GLNVTSCIEVWPSQ-DGRKTYALLLFLVLFLLPMTAISLMYAAIIYKLRTL-------------HPTQEVTMRTHKKLVRMLVVVIVTFAVCWIPYHVTFLYLDKSD------YITTEAMNAMVLFSQWLMYTNSCLNPIIYILYNANYR 270
BLAST of GCR058 vs. Ensembl Nematostella
Match: EDO36473 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIH8]) HSP 1 Score: 107.842 bits (268), Expect = 1.497e-26 Identity = 77/243 (31.69%), Postives = 127/243 (52.26%), Query Frame = 1
Query: 208 ICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADI----LCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAI 924
+ Y IF+++ +GN V ++R M+TV N FI NL++AD+ +C F +P+ Q + W +G ++C+ LQ T+ AS+ TLT + RY AI HPMKR ++ + +++ IW+ S + + P+A K F C W +K + +E F VIF+ YV+PL +I YAA+G ++ +R +T + NH + +++ K+V ML V + FAI
Sbjct: 12 VLYAAIFLISAVGNTMVCAIIVRRKKMKTVTNYFILNLAVADLALTCICIPFDIPV---QEMGYIWPYGAVMCKVLYPLQTLTLFASVYTLTAVGLTRYWAIVHPMKRQLSITQSKLVIAFIWLGSVVPVFPYANSLK-------------FTRETHTCEEYWSSK-KVREIFTAVIFVFQYVLPLGVIGTAYAAIGRELRRRKATKSG------NHKLRHLQAQEARKVVNMLSVVTMVFAI 231
BLAST of GCR058 vs. Ensembl Nematostella
Match: EDO34996 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMT8]) HSP 1 Score: 107.457 bits (267), Expect = 2.181e-26 Identity = 98/311 (31.51%), Postives = 159/311 (51.13%), Query Frame = 1
Query: 214 YGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNE------W---HFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPW---AVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGK---EYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYH 1101
Y +I + + IGN+ V+ +RT +N I N+++AD++ +LF + + + W HFG LC+ ++QG ++ + TLT IAF+R+ A+ P+K ++N K T +ILL W V+ + V + N + C+ W + + Y+ +IFI++Y IPLT I+ YA V KVW R N + N G D KK +++KML+TVVV FA+ WLP +I LLL + ++ + IVL F+ +F LG +N+ INP+IY+ +++ Y
Sbjct: 4 YLVILLASSIGNVIVIKVFSTSQRLRTEVNFLIVNMAVADVMTSLFNMSFFVRYFVREALGMPQVWFGGHFGAFLCKFVDFIQGVSMFCCVLTLTAIAFNRFFAVVFPLKSIMNPKFT----------KVLILLIWICACVFAAPTLYVMEVIVNP--EDGNYYCIENWAPTFDNETTPRYYTLLIFIVMYAIPLTFITCLYACVIRKVWIRQVPGNVTRA---NQRLEG-DMKK--RVLKMLITVVVTFALCWLPFHIYLLLVNFYYG-IYSCQISHIVL--FVGLF--LGHANSAINPWIYFMFNKDYR 291
BLAST of GCR058 vs. Ensembl Medaka
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110]) HSP 1 Score: 144.821 bits (364), Expect = 1.760e-38 Identity = 104/344 (30.23%), Postives = 187/344 (54.36%), Query Frame = 1
Query: 124 EFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQM-----KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIP 1140
N + +F IT PY++HS V + Y IF+L ++GNI V L V+ + MRTV N+FI NL+I+D+L +F +P TL+ ++ W F +C+ S ++QG +V AS+ TL IA +R+ I +P++ + + ++ IW+ + +IL P AV ++ V N +N + L+ C + N + ++ + V+F +Y++PL +I++ Y ++G+K+ V + + + + +K IK+VKML+ V + F +SWLPL+ +++ Y +++ T +Y P L SN+ +NP +Y Y++ + G +V C+S P
Sbjct: 9 SLNVSANFTNITFFPYYQHSLYVAAGYFLAYTFIFLLCMVGNILVCLIVLENRCMRTVTNLFILNLAISDLLVGIFCIPTTLVDNLITGWPFSNTVCKMSGFVQGVSVSASVFTLVAIAVERFRCIVYPLQPKLTLLVAKVAIVSIWVLAVVILCPAAVALTVEKVPFHYMVHNNDFNQTLPLY--TCYENFANP-QMRKIYTAVLFAHIYLLPLAVITLMYISIGVKLCSSVVANREPQLANIRRHGQQMVSQKKIKVVKMLVVVTLLFMVSWLPLWTLMMMTDYGGLDRDQLDLLT---SYIFPFAHWLAFSNSSVNPIVYGYYNKNFKRGFQSV--CKSRP 344
BLAST of GCR058 vs. Ensembl Medaka
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110]) HSP 1 Score: 144.821 bits (364), Expect = 2.370e-38 Identity = 104/344 (30.23%), Postives = 187/344 (54.36%), Query Frame = 1
Query: 124 EFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQM-----KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIP 1140
N + +F IT PY++HS V + Y IF+L ++GNI V L V+ + MRTV N+FI NL+I+D+L +F +P TL+ ++ W F +C+ S ++QG +V AS+ TL IA +R+ I +P++ + + ++ IW+ + +IL P AV ++ V N +N + L+ C + N + ++ + V+F +Y++PL +I++ Y ++G+K+ V + + + + +K IK+VKML+ V + F +SWLPL+ +++ Y +++ T +Y P L SN+ +NP +Y Y++ + G +V C+S P
Sbjct: 27 SLNVSANFTNITFFPYYQHSLYVAAGYFLAYTFIFLLCMVGNILVCLIVLENRCMRTVTNLFILNLAISDLLVGIFCIPTTLVDNLITGWPFSNTVCKMSGFVQGVSVSASVFTLVAIAVERFRCIVYPLQPKLTLLVAKVAIVSIWVLAVVILCPAAVALTVEKVPFHYMVHNNDFNQTLPLYT--CYENFANP-QMRKIYTAVLFAHIYLLPLAVITLMYISIGVKLCSSVVANREPQLANIRRHGQQMVSQKKIKVVKMLVVVTLLFMVSWLPLWTLMMMTDYGGLDRDQLDLLT---SYIFPFAHWLAFSNSSVNPIVYGYYNKNFKRGFQSV--CKSRP 362
BLAST of GCR058 vs. Ensembl Medaka
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110]) HSP 1 Score: 144.436 bits (363), Expect = 4.560e-38 Identity = 104/344 (30.23%), Postives = 187/344 (54.36%), Query Frame = 1
Query: 124 EFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQM-----KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIP 1140
N + +F IT PY++HS V + Y IF+L ++GNI V L V+ + MRTV N+FI NL+I+D+L +F +P TL+ ++ W F +C+ S ++QG +V AS+ TL IA +R+ I +P++ + + ++ IW+ + +IL P AV ++ V N +N + L+ C + N + ++ + V+F +Y++PL +I++ Y ++G+K+ V + + + + +K IK+VKML+ V + F +SWLPL+ +++ Y +++ T +Y P L SN+ +NP +Y Y++ + G +V C+S P
Sbjct: 23 SLNVSANFTNITFFPYYQHSLYVAAGYFLAYTFIFLLCMVGNILVCLIVLENRCMRTVTNLFILNLAISDLLVGIFCIPTTLVDNLITGWPFSNTVCKMSGFVQGVSVSASVFTLVAIAVERFRCIVYPLQPKLTLLVAKVAIVSIWVLAVVILCPAAVALTVEKVPFHYMVHNNDFNQTLPLY--TCYENFANP-QMRKIYTAVLFAHIYLLPLAVITLMYISIGVKLCSSVVANREPQLANIRRHGQQMVSQKKIKVVKMLVVVTLLFMVSWLPLWTLMMMTDYGGLDRDQLDLLT---SYIFPFAHWLAFSNSSVNPIVYGYYNKNFKRGFQSV--CKSRP 358
BLAST of GCR058 vs. Ensembl Medaka
Match: npffr1l2 (neuropeptide FF receptor 1 like 2 [Source:ZFIN;Acc:ZDB-GENE-060526-110]) HSP 1 Score: 144.436 bits (363), Expect = 4.792e-38 Identity = 104/344 (30.23%), Postives = 187/344 (54.36%), Query Frame = 1
Query: 124 EFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQM-----KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIP 1140
N + +F IT PY++HS V + Y IF+L ++GNI V L V+ + MRTV N+FI NL+I+D+L +F +P TL+ ++ W F +C+ S ++QG +V AS+ TL IA +R+ I +P++ + + ++ IW+ + +IL P AV ++ V N +N + L+ C + N + ++ + V+F +Y++PL +I++ Y ++G+K+ V + + + + +K IK+VKML+ V + F +SWLPL+ +++ Y +++ T +Y P L SN+ +NP +Y Y++ + G +V C+S P
Sbjct: 27 SLNVSANFTNITFFPYYQHSLYVAAGYFLAYTFIFLLCMVGNILVCLIVLENRCMRTVTNLFILNLAISDLLVGIFCIPTTLVDNLITGWPFSNTVCKMSGFVQGVSVSASVFTLVAIAVERFRCIVYPLQPKLTLLVAKVAIVSIWVLAVVILCPAAVALTVEKVPFHYMVHNNDFNQTLPLY--TCYENFANP-QMRKIYTAVLFAHIYLLPLAVITLMYISIGVKLCSSVVANREPQLANIRRHGQQMVSQKKIKVVKMLVVVTLLFMVSWLPLWTLMMMTDYGGLDRDQLDLLT---SYIFPFAHWLAFSNSSVNPIVYGYYNKNFKRGFQSV--CKSRP 362
BLAST of GCR058 vs. Ensembl Medaka
Match: npy2rl (neuropeptide Y receptor type 2 [Source:NCBI gene;Acc:101162714]) HSP 1 Score: 136.346 bits (342), Expect = 7.677e-36 Identity = 105/345 (30.43%), Postives = 167/345 (48.41%), Query Frame = 1
Query: 172 FKHSGLVI--CILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHR----KICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFG 1188
F S LV +L + Y I VL ++GN V+ + R ++RTV N FI NL++AD+L LP TL+ ++ EW FG+ LC PY QG V S TL +IA DR+ I + ++ + K + ++ W+ SA++ P A++ + GSF L ++C +WP K + + I+ Y +PL++IS YA ++W ++ S + N + G ++ K KML+T+VV FA+SWLP + L N ++++ L Y IF V+ + NP +Y +R Y AV CR G + ++T G
Sbjct: 41 FTSSALVCLKVVLILAYSTIIVLGVLGNSLVIYVIYRFKTLRTVTNFFIANLAVADLLVNTLCLPFTLVYTLQGEWKFGSTLCFLLPYAQGLAVHVSTVTLNVIALDRHRCIVYHLETRMRKDVCFGVIALTWVLSAVLASPLAIFREY----------GSFTLEPGHTIQVCTEKWPGKSTDGTIYSISMLILQYFLPLSIISFAYA----RIWSKLRGHVSPAEGGANSSAGSERHRRRRKTTKMLVTMVVVFAVSWLPFHAFQLATDIDNKV---LDMRDFRLLY--TIFHVVAMCSTFANPLLYGWMNRNYRAAFLAVFKCRRGADRGRSDFRARRDLTKG 366
BLAST of GCR058 vs. Planmine SMEST
Match: SMESG000046633.1 (SMESG000046633.1) HSP 1 Score: 733.791 bits (1893), Expect = 0.000e+0 Identity = 390/391 (99.74%), Postives = 391/391 (100.00%), Query Frame = 1
Query: 121 MEFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ 1293
MEFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYIIVIGIWISSAIILLPWAVYYKIQ+KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWLPLYITLLLYYYLNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ
Sbjct: 1 MEFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYIIVIGIWISSAIILLPWAVYYKIQIKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWLPLYITLLLYYYLNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ 391
BLAST of GCR058 vs. Planmine SMEST
Match: SMESG000046633.1 (SMESG000046633.1) HSP 1 Score: 666.381 bits (1718), Expect = 0.000e+0 Identity = 357/358 (99.72%), Postives = 358/358 (100.00%), Query Frame = 1
Query: 220 MIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ 1293
MIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYIIVIGIWISSAIILLPWAVYYKIQ+KVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWLPLYITLLLYYYLNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ
Sbjct: 1 MIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYIIVIGIWISSAIILLPWAVYYKIQIKVTNLTWNGSFQLHRKICLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWLPLYITLLLYYYLNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKSYKSSPTFYSARMINKQ 358
BLAST of GCR058 vs. Planmine SMEST
Match: SMESG000022910.1 (SMESG000022910.1) HSP 1 Score: 287.345 bits (734), Expect = 6.130e-94 Identity = 161/331 (48.64%), Postives = 221/331 (66.77%), Query Frame = 1
Query: 172 FKHSGLVICILSICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKI-CLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRV---STDNSGKKFCL-NHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNG 1149
F++S I IL + YG+I ++ IIGN+ V+ TV+ H M+TVINI ICNLS+AD+LCA+FVLP+ LQSI EW +G +LCR++PYLQG +VC SI TLT++AFDRYVAICHP+ R++N I+ IWI SAI+LLPWA+YY+ +M H KI C+ WP+ + ++YF+ VIFI+ Y+IPL LIS FY A+ ++++R S + K+ L + GGI R KSI++VKML VVV FA+SWLPLYI + YY + W+ ++ Y +PIFQ++ ASN+CINP+IY YS+ Y DG VI C+ N
Sbjct: 9 FRYSIAFITILGVSYGLILLMAIIGNLIVITTVLWHPCMKTVINILICNLSVADLLCAIFVLPMNFLQSITKEWLYGEILCRSTPYLQGVSVCTSIMTLTVVAFDRYVAICHPLHRILNISKATSIISMIWILSAILLLPWALYYRTEMD----------NNHTKIVCVQAWPDNADERDYFIFVIFIMCYIIPLFLISCFYFAIASRIYKRANANSNETQSKRIPLIKLSSGGISRTKSIRIVKMLAIVVVTFAVSWLPLYIVFIFLYYSDKNKWSPQFLNVLFDYLIPIFQLISASNSCINPWIYCLYSQTYRDGFRNVIICKKATLND 329
BLAST of GCR058 vs. Planmine SMEST
Match: SMESG000022910.1 (SMESG000022910.1) HSP 1 Score: 173.711 bits (439), Expect = 2.464e-51 Identity = 106/234 (45.30%), Postives = 147/234 (62.82%), Query Frame = 1
Query: 463 TLTIIAFDRYVAICHPMKRVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKI-CLMEWPNKGEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRV---STDNSGKKFCL-NHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNG 1149
TLT++AFDRYVAICHP+ R++N I+ IWI SAI+LLPWA+YY+ +M H KI C+ WP+ + ++YF+ VIFI+ Y+IPL LIS FY A+ ++++R S + K+ L + GGI R KSI++VKML VVV FA+SWLPLYI + YY + W+ ++ Y +PIFQ++ ASN+CINP+IY YS+ Y DG VI C+ N
Sbjct: 2 TLTVVAFDRYVAICHPLHRILNISKATSIISMIWILSAILLLPWALYYRTEMD----------NNHTKIVCVQAWPDNADERDYFIFVIFIMCYIIPLFLISCFYFAIASRIYKRANANSNETQSKRIPLIKLSSGGISRTKSIRIVKMLAIVVVTFAVSWLPLYIVFIFLYYSDKNKWSPQFLNVLFDYLIPIFQLISASNSCINPWIYCLYSQTYRDGFRNVIICKKATLND 225
BLAST of GCR058 vs. Planmine SMEST
Match: SMESG000023355.1 (SMESG000023355.1) HSP 1 Score: 175.637 bits (444), Expect = 1.494e-50 Identity = 121/349 (34.67%), Postives = 194/349 (55.59%), Query Frame = 1
Query: 208 ICYGMIFVLTIIGNIAVLLTVIRHVSMRTVINIFICNLSIADILCALFVLPITLLQSIHNEWHFGTLLCRASPYLQGTTVCASISTLTIIAFDRYVAICHPMK-RVINKKTTYXXXXXXXXXXXXXLLPWAVYYKIQMKVTNLTWNGSFQLHRKICLMEWPNK--GEGKEYFLGVIFIILYVIPLTLISIFYAAVGLKVWQRVSTDNSGKKFCLNHTCGGIDRKKSIKLVKMLLTVVVFFAISWXXXXXXXXXXXXXNNQSWNVNVKTIVLTYFLPIFQVLGASNNCINPFIYYNYSRKYHDGINAVICCRSIPGNGYLQGASGYNVTFGTNSCSLRKANEGLEITNKS 1245
+ Y ++F I GN V+LT++R+ M+ VIN FI NLSIADI A+F+LP TL+ +I W G +C++ P++QG T+ S++TL I+ +RY AIC+P++ R I+K+ II+I IW+ + I++LPW ++Y + L +++ + C +WP K YF V+F +LY+ PL +I I Y+ +G V S + C I+++K IK++KML+ VV+ FA SWLPLY + Y+ + + ++ +P+ Q LG+SN+C+NP+IYY +SR + G + C Y Y + +N + NE L I+ K
Sbjct: 30 VIYSIVFFFIIAGNTFVILTIVRNAEMKIVINFFILNLSIADICVAIFILPATLIDNITKGWVLGNFMCKSVPFIQGVTISTSVNTLVAISIERYAAICYPLRQRWISKRKCVIILILIWLEALILILPWLLFYTHERTDPILETENIYKIWYR-CYQKWPLNMLHLQKWYFAIVMFTLLYLCPLVIICISYSLIGFAV--------SKRNLLFQRKCNVIEKRK-IKVIKMLVIVVIMFAFSWLPLYAYNMKQYFGPSTEEQSEFEYSIV---VPMIQWLGSSNSCVNPWIYYFFSRNFRVGFMRYLRCLFGKKESYQSLILTYQI--PSNLRTNNPDNETLTISKKK 363 The following BLAST results are available for this feature:
BLAST of GCR058 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of GCR058 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of GCR058 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of GCR058 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of GCR058 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of GCR058 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of GCR058 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of GCR058 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of GCR058 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of GCR058 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of GCR058 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of GCR058 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of GCR058 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of GCR058 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004162 ID=SMED30004162|Name=GCR058|organism=Schmidtea mediterranea sexual|type=transcript|length=1308bpback to top protein sequence of SMED30004162-orf-1 >SMED30004162-orf-1 ID=SMED30004162-orf-1|Name=SMED30004162-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=392bp MEFNNNQSFPIITRLPYFKHSGLVICILSICYGMIFVLTIIGNIAVLLTVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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