EZH
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30004132 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of EZH vs. Ensembl Human
Match: EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:HGNC Symbol;Acc:HGNC:3527]) HSP 1 Score: 243.047 bits (619), Expect = 8.703e-69 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 548 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 693 HSP 2 Score: 126.331 bits (316), Expect = 2.838e-29 Identity = 95/328 (28.96%), Postives = 157/328 (47.87%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ +++ K + + P + S + + DF ++ ++ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L D++ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPV--HILTSCSVTSDLDFPTQVIP------LKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPS-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 317 HSP 3 Score: 63.1586 bits (152), Expect = 1.489e-9 Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 2
Query: 1538 NRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 504 NRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 538
BLAST of EZH vs. Ensembl Human
Match: EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:HGNC Symbol;Acc:HGNC:3527]) HSP 1 Score: 243.047 bits (619), Expect = 8.703e-69 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 548 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 693 HSP 2 Score: 126.331 bits (316), Expect = 2.838e-29 Identity = 95/328 (28.96%), Postives = 157/328 (47.87%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ +++ K + + P + S + + DF ++ ++ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L D++ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPV--HILTSCSVTSDLDFPTQVIP------LKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPS-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 317 HSP 3 Score: 63.1586 bits (152), Expect = 1.489e-9 Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 2
Query: 1538 NRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 504 NRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 538
BLAST of EZH vs. Ensembl Human
Match: EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:HGNC Symbol;Acc:HGNC:3527]) HSP 1 Score: 242.662 bits (618), Expect = 1.322e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 560 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 705 HSP 2 Score: 130.954 bits (328), Expect = 1.027e-30 Identity = 75/203 (36.95%), Postives = 100/203 (49.26%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E +M VL+ + ++CA+++L+ +K+C QVY + K+ + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 381 MKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPAPAEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 550 HSP 3 Score: 97.8265 bits (242), Expect = 2.897e-20 Identity = 72/244 (29.51%), Postives = 117/244 (47.95%), Query Frame = 2
Query: 341 IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
I+ + ++ S + L+ VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L D++ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 65 IQPVHILTSVSSLRGTREVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPS-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 287
BLAST of EZH vs. Ensembl Human
Match: EZH1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:HGNC Symbol;Acc:HGNC:3526]) HSP 1 Score: 241.891 bits (616), Expect = 1.740e-68 Identity = 110/147 (74.83%), Postives = 127/147 (86.39%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIET 2326
++CKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGE+ISQDEADRRGK+YDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+AG+ELFFDYRY + LKYVGIERE +
Sbjct: 530 VSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDV 676 HSP 2 Score: 121.324 bits (303), Expect = 1.134e-27 Identity = 69/190 (36.32%), Postives = 99/190 (52.11%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+WT +E ++ V + ++C++++LL +K+C QV+ + KE L + T + +N + ++KK K R + + + K N S +V + Y PCDHP PCD C C NFCEKFCQC+ C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 363 EWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAV---KESLILKLP-----TDELMNPS---QKKKRKHRL-----WAAHCRKIQLKKDN---SSTQVYN-------------YQPCDHPDRPCDSTCPCIMTQNFCEKFCQCNPDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCG 520 HSP 3 Score: 119.013 bits (297), Expect = 5.624e-27 Identity = 80/261 (30.65%), Postives = 122/261 (46.74%), Query Frame = 2
Query: 323 VRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGED---HDPKFIEELLRNYEGRVHGN---FPFD--FNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYP-----------------------EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKL 1012
+R L + +P+M SW+PLQQNF VEDE L NIPYMG++ D FIEEL+ NY+G+VHG P ++ ++L+ LN+ D + N ++ D P ++IF AIA F + + RY E+ +S+ +A TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ +++ N PC +CF L
Sbjct: 29 MRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEEGHNDTSDGKQDDSKEDLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALP--PQCTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLL 270
BLAST of EZH vs. Ensembl Human
Match: EZH2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:HGNC Symbol;Acc:HGNC:3527]) HSP 1 Score: 243.047 bits (619), Expect = 1.943e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 590 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 735 HSP 2 Score: 131.339 bits (329), Expect = 9.297e-31 Identity = 75/203 (36.95%), Postives = 100/203 (49.26%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E +M VL+ + ++CA+++L+ +K+C QVY + K+ + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 411 MKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPAPAEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 580 HSP 3 Score: 127.102 bits (318), Expect = 1.997e-29 Identity = 95/328 (28.96%), Postives = 157/328 (47.87%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ +++ K + + P + S + + DF ++ ++ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L D++ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPV--HILTSCSVTSDLDFPTQVIP------LKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPS-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 317
BLAST of EZH vs. Ensembl Celegans
Match: mes-2 (Histone-lysine N-methyltransferase mes-2 [Source:UniProtKB/Swiss-Prot;Acc:O17514]) HSP 1 Score: 124.02 bits (310), Expect = 7.822e-29 Identity = 62/156 (39.74%), Postives = 93/156 (59.62%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANH-SVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQS 2350
I C+N + R +K S +AG G++ E K+EFI EY GE IS DEA+RRG IYD+ S++FN+ +D+ + GN RFANH S NP C A+ ++V G+HRIG +AK ++ +EL FDY Y Q+ + ++ + ++ S +S
Sbjct: 608 IKCRNFGMTRMIQKRTYCGPSKIAGNGLFLLEPAEKDEFITEYTGERISDDEAERRGAIYDRYQCSYIFNIETGGAIDSYKIGNLARFANHDSKNPTCYARTMVVAGEHRIGFYAKRRLEISEELTFDYSYSGEHQIAFRMVQTKERSEKPSRPKS 763 HSP 2 Score: 56.6102 bits (135), Expect = 7.635e-8 Identity = 32/70 (45.71%), Postives = 38/70 (54.29%), Query Frame = 2
Query: 1442 CDHPGFPCD---EKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCR-GHCNTKQCPCFLANRECDQDLCQCC 1639
C H G PC+ E C CR+ G C C+C +CS RF GC C G C TK C C+ AN EC+ C C
Sbjct: 531 CRHAG-PCNATAENCACRENG-VCSYMCKCDINCSQRFPGCNCAAGQCYTKACQCYRANWECNPMTCNMC 598
BLAST of EZH vs. Ensembl Celegans
Match: set-2 (Histone-lysine N-methyltransferase set-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18221]) HSP 1 Score: 107.071 bits (266), Expect = 1.520e-23 Identity = 49/121 (40.50%), Postives = 77/121 (63.64%), Query Frame = 2
Query: 1919 QRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDK--TMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRY 2275
++K + + S + GWG+Y E I +E I EY G+ I A+ R K Y++ SS+LF ++ V+DAT++GN RF NHS PNC AK+L + G+ RI I+++ IK G+E+ +DY++
Sbjct: 598 RKKMIKFARSRIHGWGLYAMESIAPDEMIVEYIGQTIRSLVAEEREKAYERRGIGSSYLFRIDLHHVIDATKRGNFARFINHSCQPNCYAKVLTIEGEKRIVIYSRTIIKKGEEITYDYKF 718
BLAST of EZH vs. Ensembl Celegans
Match: set-2 (Histone-lysine N-methyltransferase set-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18221]) HSP 1 Score: 103.99 bits (258), Expect = 2.581e-22 Identity = 52/128 (40.62%), Postives = 80/128 (62.50%), Query Frame = 2
Query: 1919 QRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDK--TMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLK 2296
++K + + S + GWG+Y E I +E I EY G+ I A+ R K Y++ SS+LF ++ V+DAT++GN RF NHS PNC AK+L + G+ RI I+++ IK G+E+ +DY++ P E K
Sbjct: 1366 RKKMIKFARSRIHGWGLYAMESIAPDEMIVEYIGQTIRSLVAEEREKAYERRGIGSSYLFRIDLHHVIDATKRGNFARFINHSCQPNCYAKVLTIEGEKRIVIYSRTIIKKGEEITYDYKF-PIEDDK 1492
BLAST of EZH vs. Ensembl Celegans
Match: set-2 (Histone-lysine N-methyltransferase set-2 [Source:UniProtKB/Swiss-Prot;Acc:Q18221]) HSP 1 Score: 103.99 bits (258), Expect = 2.583e-22 Identity = 52/128 (40.62%), Postives = 80/128 (62.50%), Query Frame = 2
Query: 1919 QRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDK--TMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLK 2296
++K + + S + GWG+Y E I +E I EY G+ I A+ R K Y++ SS+LF ++ V+DAT++GN RF NHS PNC AK+L + G+ RI I+++ IK G+E+ +DY++ P E K
Sbjct: 1369 RKKMIKFARSRIHGWGLYAMESIAPDEMIVEYIGQTIRSLVAEEREKAYERRGIGSSYLFRIDLHHVIDATKRGNFARFINHSCQPNCYAKVLTIEGEKRIVIYSRTIIKKGEEITYDYKF-PIEDDK 1495
BLAST of EZH vs. Ensembl Celegans
Match: set-12 (Histone-lysine N-methyltransferase [Source:UniProtKB/TrEMBL;Acc:Q21404]) HSP 1 Score: 83.9593 bits (206), Expect = 7.253e-17 Identity = 45/108 (41.67%), Postives = 61/108 (56.48%), Query Frame = 2
Query: 1958 GWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIY--DKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGD--HRIGIFAKHNIKAGDELFFDY 2269
G G+ E+I + I EY GE I++ E ++R K Y D S+ F + +++ VD TRKGN RF NHS NPN K+ V +GIFA IK G+E+ FDY
Sbjct: 107 GHGLRATEEIATGKLILEYRGEAITKAEHNKRVKRYKKDGIKHSYSFEVGRNYYVDPTRKGNSARFINHSCNPNALVKVWTVPDRPMKSLGIFASKVIKPGEEITFDY 214
BLAST of EZH vs. Ensembl Fly
Match: E(z) (gene:FBgn0000629 transcript:FBtr0273338) HSP 1 Score: 250.751 bits (639), Expect = 1.901e-71 Identity = 114/146 (78.08%), Postives = 128/146 (87.67%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
ITCKN+ +QRG KHLLM+ SD+AGWGI+ KE KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHS+NPNC AK+++V GDHRIGIFAK I+ G+ELFFDYRYGPTEQLK+VGIERE+E
Sbjct: 613 ITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREME 758 HSP 2 Score: 182.57 bits (462), Expect = 1.176e-47 Identity = 174/663 (26.24%), Postives = 260/663 (39.22%), Query Frame = 2
Query: 62 NKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMV---SKMKSLSPF------CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE-----------------------DHDPKFIEE---------LLRNYEGRVHGNFPFDFNENQLIDLLKTLN-------------------EKYDW---------------YKLADNKVKKHQEKIEN-FDYP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSS--DVIGPCEKNCFKL---ELTNIEGDSLNENINHC------------------DYNQ----------------------------------WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYH--PCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
N ++ + VK EY ++ Q K+ D++++ N D + + + SK+ P+ C K +Y+ I S K + ++ + +P M +WAP QQNF VEDE L NIPYMG+ D DP F+++ L+R+Y + P + L K+ EK D + D VK E++++ +P IIF+AI+ F D +L +Y+E L+E + + TPN+D ++A S+ R+ H+F +L CRRCFKYDC +H + Y PC +C+ L + DS I+ C D+N WT +++ + VL ++ +YCA++ + +K+C QVY + Q KED E + D + T + K++ R + +L KD + SN Y+ PCDHPG PCD C+C Q NFCEKFC CS C NRF GC+C+ CNTKQCPC+LA RECD DLCQ C
Sbjct: 2 NSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYN-------GIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIE---LTEHQDPERPQECTPNIDG----------IKA----ESV---SRERTMHSFHTLFCRRCFKYDCFLHRLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQ---KEDAE--FSFEDLRQDFTPPRKKKKKQRLWSLHCRKI----QLKKD------------------------SSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACG 604
BLAST of EZH vs. Ensembl Fly
Match: E(z) (gene:FBgn0000629 transcript:FBtr0076279) HSP 1 Score: 250.751 bits (639), Expect = 1.901e-71 Identity = 114/146 (78.08%), Postives = 128/146 (87.67%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
ITCKN+ +QRG KHLLM+ SD+AGWGI+ KE KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHS+NPNC AK+++V GDHRIGIFAK I+ G+ELFFDYRYGPTEQLK+VGIERE+E
Sbjct: 613 ITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREME 758 HSP 2 Score: 182.57 bits (462), Expect = 1.176e-47 Identity = 174/663 (26.24%), Postives = 260/663 (39.22%), Query Frame = 2
Query: 62 NKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMV---SKMKSLSPF------CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE-----------------------DHDPKFIEE---------LLRNYEGRVHGNFPFDFNENQLIDLLKTLN-------------------EKYDW---------------YKLADNKVKKHQEKIEN-FDYP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSS--DVIGPCEKNCFKL---ELTNIEGDSLNENINHC------------------DYNQ----------------------------------WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYH--PCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
N ++ + VK EY ++ Q K+ D++++ N D + + + SK+ P+ C K +Y+ I S K + ++ + +P M +WAP QQNF VEDE L NIPYMG+ D DP F+++ L+R+Y + P + L K+ EK D + D VK E++++ +P IIF+AI+ F D +L +Y+E L+E + + TPN+D ++A S+ R+ H+F +L CRRCFKYDC +H + Y PC +C+ L + DS I+ C D+N WT +++ + VL ++ +YCA++ + +K+C QVY + Q KED E + D + T + K++ R + +L KD + SN Y+ PCDHPG PCD C+C Q NFCEKFC CS C NRF GC+C+ CNTKQCPC+LA RECD DLCQ C
Sbjct: 2 NSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYN-------GIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIE---LTEHQDPERPQECTPNIDG----------IKA----ESV---SRERTMHSFHTLFCRRCFKYDCFLHRLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQ---KEDAE--FSFEDLRQDFTPPRKKKKKQRLWSLHCRKI----QLKKD------------------------SSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACG 604
BLAST of EZH vs. Ensembl Fly
Match: E(z) (gene:FBgn0000629 transcript:FBtr0334067) HSP 1 Score: 250.751 bits (639), Expect = 2.309e-71 Identity = 114/146 (78.08%), Postives = 128/146 (87.67%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
ITCKN+ +QRG KHLLM+ SD+AGWGI+ KE KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHS+NPNC AK+++V GDHRIGIFAK I+ G+ELFFDYRYGPTEQLK+VGIERE+E
Sbjct: 618 ITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREME 763 HSP 2 Score: 181.03 bits (458), Expect = 4.009e-47 Identity = 173/668 (25.90%), Postives = 262/668 (39.22%), Query Frame = 2
Query: 62 NKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMV---SKMKSLSPF------CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE-----------------------DHDPKFIEE---------LLRNYEGRVHGNFPFDFNENQLIDLLKTLN-------------------EKYDW---------------YKLADNKVKKHQEKIEN-FDYP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPY-------RSSKSMWNNANAYSSDVIGPCEKNCFKL---ELTNIEGDSLNENINHC------------------DYNQ----------------------------------WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYH--PCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
N ++ + VK EY ++ Q K+ D++++ N D + + + SK+ P+ C K +Y+ I S K + ++ + +P M +WAP QQNF VEDE L NIPYMG+ D DP F+++ L+R+Y + P + L K+ EK D + D VK E++++ +P IIF+AI+ F D +L +Y+E L+E + + TPN+D ++A S+ R+ H+F +L CRRCFKYDC +H + + ++ PC +C+ L + DS I+ C D+N WT +++ + VL ++ +YCA++ + +K+C QVY + Q KED E + D + T + K++ R + +L KD + SN Y+ PCDHPG PCD C+C Q NFCEKFC CS C NRF GC+C+ CNTKQCPC+LA RECD DLCQ C
Sbjct: 2 NSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYN-------GIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIE---LTEHQDPERPQECTPNIDG----------IKA----ESV---SRERTMHSFHTLFCRRCFKYDCFLHRHHVQGLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQ---KEDAE--FSFEDLRQDFTPPRKKKKKQRLWSLHCRKI----QLKKD------------------------SSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACG 609
BLAST of EZH vs. Ensembl Fly
Match: trr (gene:FBgn0023518 transcript:FBtr0070362) HSP 1 Score: 97.4413 bits (241), Expect = 3.599e-20 Identity = 49/119 (41.18%), Postives = 73/119 (61.34%), Query Frame = 2
Query: 1922 RKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYD-KTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRY 2275
R ++ ++ S + G G+Y DI K+ I EY GE+I + ++ R K Y+ K ++F L++ VVDAT G R+ NHS NPNC +I+ V+ D RI IFAK I G+EL +DY++
Sbjct: 2269 RNNVYLARSKIQGLGLYAARDIEKHTMIIEYIGEVIRTEVSEIREKQYESKNRGIYMFRLDEDRVVDATLSGGLARYINHSCNPNCVTEIVEVDRDVRIIIFAKRKIYRGEELSYDYKF 2387
BLAST of EZH vs. Ensembl Fly
Match: trr (gene:FBgn0023518 transcript:FBtr0070363) HSP 1 Score: 97.4413 bits (241), Expect = 3.606e-20 Identity = 49/119 (41.18%), Postives = 73/119 (61.34%), Query Frame = 2
Query: 1922 RKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYD-KTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRY 2275
R ++ ++ S + G G+Y DI K+ I EY GE+I + ++ R K Y+ K ++F L++ VVDAT G R+ NHS NPNC +I+ V+ D RI IFAK I G+EL +DY++
Sbjct: 2290 RNNVYLARSKIQGLGLYAARDIEKHTMIIEYIGEVIRTEVSEIREKQYESKNRGIYMFRLDEDRVVDATLSGGLARYINHSCNPNCVTEIVEVDRDVRIIIFAKRKIYRGEELSYDYKF 2408
BLAST of EZH vs. Ensembl Zebrafish
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:768133]) HSP 1 Score: 243.817 bits (621), Expect = 8.445e-69 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ KE + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 613 VSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 758 HSP 2 Score: 132.109 bits (331), Expect = 3.785e-31 Identity = 73/191 (38.22%), Postives = 96/191 (50.26%), Query Frame = 2
Query: 1076 WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + A D T Q ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 446 WSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIARAPAVDENTPQ--------RKKKRKHRLW--------------------ATHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 603 HSP 3 Score: 124.79 bits (312), Expect = 6.490e-29 Identity = 98/355 (27.61%), Postives = 165/355 (46.48%), Query Frame = 2
Query: 53 RTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPY-----IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNE----------------KYDWYKLADNK--VKKHQEKIENFDY---------PEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
R + K +C + VK EY L+ ++ D+V+ + N I E+ ++++ L + S E ++S + +++P + +PVM SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ LN+ K++ L D K + H++++ + + + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N + PC C+
Sbjct: 6 RKSEKGPVC--WRRRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTDILNQEWKLRRIQPVHIMTPVSSLRGTRECTVDSGFSEFSRQVIPLKTLNAVASVPVMYSWSPLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALNQYSDNEEDDEEDDHHDYKFEKMDLCDGKDDAEDHKDQLSSESHNNDGSKKFPSDKIFEAISSMFPDKGSTEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNMENLVDSKPCGIYCY 338
BLAST of EZH vs. Ensembl Zebrafish
Match: ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:ZFIN;Acc:ZDB-GENE-050114-1]) HSP 1 Score: 243.047 bits (619), Expect = 1.355e-68 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGE+ISQDEADRRG+IYDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIEREIE
Sbjct: 602 VSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKEPVQKNEFISEYCGELISQDEADRRGRIYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQQGEELFFDYRYSQADALKYVGIEREIE 747 HSP 2 Score: 137.117 bits (344), Expect = 8.627e-33 Identity = 75/192 (39.06%), Postives = 100/192 (52.08%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
QW+ +E ++ VL + ++C++++L+ +K+C +VY + KE L + M D+ GI +K K++ R KI +L KD SNQ Y PCDHP PCD C C NFCEKFCQC R C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 439 QWSGAEESLFRVLHGTYYNNFCSIARLIGTKTCREVYEFAV---KEVLIDRMPLEDS----------GISPQKKKRKHRLWAKI-QLKKD------------------------NSSNQVYNYQPCDHPEHPCDSSCPCVITQNFCEKFCQCDRECQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCMTCG 592 HSP 3 Score: 106.301 bits (264), Expect = 4.840e-23 Identity = 83/278 (29.86%), Postives = 122/278 (43.88%), Query Frame = 2
Query: 323 VRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNE-------------------------------------------KYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLE 1015
+R L + +P M SW+PLQQNF VEDE L NIPYMG+ + D F+EEL+ NY+G VHG+ F N+ +L++ L++ K W A+ + +KI + + IF AIA F +L +YL+ LL + TPN+D F ++ Q E S+ H+F +L CRRCFKYDC +HP+ SS +++ + PC +CF L+
Sbjct: 105 MRPLTTVAGIPFMYSWSPLQQNFMVEDETFLHNIPYMGDEVLEQDEAFLEELIDNYDG-VHGDREGGFINDEIFKELVEALSQYSDPEEEEEEKEPTDAAENKQEEEKEMQKSAAEAPEESKTAFFKRKWRNTAEGRELSANKKIPH----DKIFTAIASMFPYKGTMQELKDKYLD--LLEPSNHVKLPPLCTPNMDG--------PFAKSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFHSSPNVYKRKSKEIHMETEPCGLDCFLLQ 358
BLAST of EZH vs. Ensembl Zebrafish
Match: ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:ZFIN;Acc:ZDB-GENE-050114-1]) HSP 1 Score: 242.662 bits (618), Expect = 1.873e-68 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGE+ISQDEADRRG+IYDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIEREIE
Sbjct: 609 VSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKEPVQKNEFISEYCGELISQDEADRRGRIYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQQGEELFFDYRYSQADALKYVGIEREIE 754 HSP 2 Score: 136.732 bits (343), Expect = 1.086e-32 Identity = 75/192 (39.06%), Postives = 100/192 (52.08%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
QW+ +E ++ VL + ++C++++L+ +K+C +VY + KE L + M D+ GI +K K++ R KI +L KD SNQ Y PCDHP PCD C C NFCEKFCQC R C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 446 QWSGAEESLFRVLHGTYYNNFCSIARLIGTKTCREVYEFAV---KEVLIDRMPLEDS----------GISPQKKKRKHRLWAKI-QLKKD------------------------NSSNQVYNYQPCDHPEHPCDSSCPCVITQNFCEKFCQCDRECQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCMTCG 599 HSP 3 Score: 103.219 bits (256), Expect = 4.205e-22 Identity = 85/285 (29.82%), Postives = 120/285 (42.11%), Query Frame = 2
Query: 323 VRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGN--------------------------------------FPFDFNEN-------------QLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLE 1015
+R L + +P M SW+PLQQNF VEDE L NIPYMG+ + D F+EEL+ NY+G VHG+ P D EN + + KT K W A+ + +KI + + IF AIA F +L +YL+ LL + TPN+D F ++ Q E S+ H+F +L CRRCFKYDC +HP+ SS +++ + PC +CF L+
Sbjct: 105 MRPLTTVAGIPFMYSWSPLQQNFMVEDETFLHNIPYMGDEVLEQDEAFLEELIDNYDG-VHGDRVSCTFLIEGGFINDEIFKELVEALSQYSDPEEEEEEKEPTDAAENKQEEEKEMQKSAAEAPEESKTAFFKRKWRNTAEGRELSANKKIPH----DKIFTAIASMFPYKGTMQELKDKYLD--LLEPSNHVKLPPLCTPNMDG--------PFAKSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFHSSPNVYKRKSKEIHMETEPCGLDCFLLQ 365
BLAST of EZH vs. Ensembl Zebrafish
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:768133]) HSP 1 Score: 229.18 bits (583), Expect = 1.825e-63 Identity = 104/138 (75.36%), Postives = 119/138 (86.23%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKY 2299
++CKN +IQRG +KHLL++ SDVAGWGI+ KE + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKY
Sbjct: 613 VSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKY 750 HSP 2 Score: 132.109 bits (331), Expect = 3.980e-31 Identity = 73/191 (38.22%), Postives = 96/191 (50.26%), Query Frame = 2
Query: 1076 WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + A D T Q ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 446 WSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIARAPAVDENTPQ--------RKKKRKHRLW--------------------ATHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 603 HSP 3 Score: 125.176 bits (313), Expect = 5.555e-29 Identity = 93/344 (27.03%), Postives = 165/344 (47.97%), Query Frame = 2
Query: 53 RTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPY-----IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASD----GVETVTPNLDNEEEIREMEKFLRATQPENSIIEH-------------KRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
R + K +C + VK EY L+ ++ D+V+ + N I E+ ++++ L + S E ++S + +++P + +PVM SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ LN+ +DN+ ++ ++ + ++ + + D ED+ ++ S+K SD + ++ P+ + EE++E K L Q ++ +R+ + H+F +L CRRCFKYDC +HP+ ++ + + N + PC C+
Sbjct: 6 RKSEKGPVC--WRRRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTDILNQEWKLRRIQPVHIMTPVSSLRGTRECTVDSGFSEFSRQVIPLKTLNAVASVPVMYSWSPLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALNQ------YSDNEEDDEEDDHHDYKFEKM---DLCDGKDDAEDHKDQLSSESHNNDGSKKFPSDKIFEAISSMFPDKGSTEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNMENLVDSKPCGIYCY 338
BLAST of EZH vs. Ensembl Zebrafish
Match: suv39h1b (suppressor of variegation 3-9 homolog 1b [Source:ZFIN;Acc:ZDB-GENE-030131-5105]) HSP 1 Score: 107.842 bits (268), Expect = 2.752e-24 Identity = 60/134 (44.78%), Postives = 79/134 (58.96%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVA-GWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNL---NQSFVVDATRKGNKIRFANHSVNPNC-CAKILLVNGDH---RIGIFAKHNIKAGDELFFDY 2269
C N +QRG R L + +D GWG+ T E I KN F+ EY GEII+ +EA+RRG +YDK +++LF+L + + VDA GN F NHS +PN + + N D RI FA IKAG+EL FDY
Sbjct: 251 CSNRVVQRGIRYSLCIFRTDNGRGWGVRTMERIRKNTFVMEYVGEIITTEEAERRGHVYDKEGATYLFDLDYVDDEYTVDAAHYGNISHFVNHSCDPNLQVYNVFIDNLDERLPRIAFFATRGIKAGEELTFDY 384
BLAST of EZH vs. Ensembl Xenopus
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:550047]) HSP 1 Score: 243.047 bits (619), Expect = 1.186e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 574 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 719 HSP 2 Score: 125.561 bits (314), Expect = 5.504e-29 Identity = 95/336 (28.27%), Postives = 158/336 (47.02%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLA------------------DNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKL 1012
VK EY L+ ++ D+V+ + N I E+ +++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + D+ DN + + + F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N +++ PC +C++L
Sbjct: 34 VKSEYMRLRQLKRFRRADEVKSMFNTNRQKIMERTEILNQEWKQRRIQPVHIMTTVSSLRGTRECSVTSDLDFPKQVIPLKT----LTAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALAQYSDYEDDEDGDDNQDDERDDTAKDQDDNMEDQETQPLRKFPS-DKIFEAISSMFPDKGTLEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNNEAANDGKPCGPHCYQL 344 HSP 3 Score: 63.5438 bits (153), Expect = 1.265e-9 Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 2
Query: 1538 NRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 530 NRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 564
BLAST of EZH vs. Ensembl Xenopus
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:550047]) HSP 1 Score: 242.276 bits (617), Expect = 1.261e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 548 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 693 HSP 2 Score: 130.183 bits (326), Expect = 1.609e-30 Identity = 74/203 (36.45%), Postives = 101/203 (49.75%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 369 MKPNIEPPENVEWSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVIAEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 538 HSP 3 Score: 99.7525 bits (247), Expect = 6.179e-21 Identity = 69/233 (29.61%), Postives = 118/233 (50.64%), Query Frame = 2
Query: 341 IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNE----KYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKL 1012
I+ + +M + + L+ VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + + D + ++ + F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N +++ PC +C++L
Sbjct: 65 IQPVHIMTTVSSLRGTREVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALAQYSDYEDDEDGDDNQDDERDDTPLRKFPS-DKIFEAISSMFPDKGTLEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNNEAANDGKPCGPHCYQL 276
BLAST of EZH vs. Ensembl Xenopus
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:550047]) HSP 1 Score: 241.891 bits (616), Expect = 1.356e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 521 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 666 HSP 2 Score: 208.764 bits (530), Expect = 7.143e-57 Identity = 165/579 (28.50%), Postives = 251/579 (43.35%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPFCEKVRFSQTYSE-HDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK------------------------------------------LELTNIEGD-------------SLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
VK EY L+ ++ D+V+ + N I E+ +++ K + + P V T S EV I K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + Y ++ + + D EAF L RY E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N +++ PC +C++ LE + + D + NI + +W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + + A D T ++KK K R DL + + ++ PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKSMFNTNRQKIMERTEILNQEWKQRRIQP----VHIMTTVSSLRGTREVIIPLKT-------LTAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALAQ----YSDYEDDEDGDDNQDDERDDTAKDQDDNMEAF--------LFFRYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNNEAANDGKPCGPHCYQLLVCCLEFILYALLMCLSKSLGRRRGRLPNNTSRPSTPTVNVLEAKDTDSDREAEANSRCQTPIKMKPNIEPPENVEWSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVIAEDVDTPP--------RKKKRKHRY-----------DLHFITFSLSV----------------------PCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 511
BLAST of EZH vs. Ensembl Xenopus
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:550047]) HSP 1 Score: 243.047 bits (619), Expect = 1.378e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 589 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 734 HSP 2 Score: 126.331 bits (316), Expect = 3.334e-29 Identity = 95/336 (28.27%), Postives = 158/336 (47.02%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLA------------------DNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKL 1012
VK EY L+ ++ D+V+ + N I E+ +++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + D+ DN + + + F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N +++ PC +C++L
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKSMFNTNRQKIMERTEILNQEWKQRRIQPVHIMTTVSSLRGTRECSVTSDLDFPKQVIPLKT----LTAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALAQYSDYEDDEDGDDNQDDERDDTAKDQDDNMEDQETQPLRKFPS-DKIFEAISSMFPDKGTLEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNNEAANDGKPCGPHCYQL 329 HSP 3 Score: 124.79 bits (312), Expect = 9.890e-29 Identity = 70/201 (34.83%), Postives = 96/201 (47.76%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + + A D T ++KK K R D I S PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 422 MKPNIEPPENVEWSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVIAEDVDTPP--------RKKKRKHR-----------------------YDLHFITFS----------LSVPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 579
BLAST of EZH vs. Ensembl Xenopus
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:550047]) HSP 1 Score: 243.047 bits (619), Expect = 2.577e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 616 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 761 HSP 2 Score: 130.954 bits (328), Expect = 1.254e-30 Identity = 74/203 (36.45%), Postives = 101/203 (49.75%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 437 MKPNIEPPENVEWSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVIAEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 606 HSP 3 Score: 125.946 bits (315), Expect = 4.245e-29 Identity = 95/336 (28.27%), Postives = 158/336 (47.02%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLA------------------DNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKL 1012
VK EY L+ ++ D+V+ + N I E+ +++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + D+ DN + + + F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N +++ PC +C++L
Sbjct: 34 VKSEYMRLRQLKRFRRADEVKSMFNTNRQKIMERTEILNQEWKQRRIQPVHIMTTVSSLRGTRECSVTSDLDFPKQVIPLKT----LTAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALAQYSDYEDDEDGDDNQDDERDDTAKDQDDNMEDQETQPLRKFPS-DKIFEAISSMFPDKGTLEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNNEAANDGKPCGPHCYQL 344
BLAST of EZH vs. Ensembl Mouse
Match: Ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:MGI Symbol;Acc:MGI:107940]) HSP 1 Score: 243.047 bits (619), Expect = 5.944e-69 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 557 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 702 HSP 2 Score: 124.79 bits (312), Expect = 7.117e-29 Identity = 97/333 (29.13%), Postives = 155/333 (46.55%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ ++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L DN+ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKTMFSSNRQKILERTETLNQEWKQRRIQPVHIMTSVSSLRGTRECSVTSDLDFPAQVIPLKT----LNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQKDLEDNRDDKETCPPRKFPA-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 326 HSP 3 Score: 63.1586 bits (152), Expect = 1.075e-9 Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 2
Query: 1538 NRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 513 NRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 547
BLAST of EZH vs. Ensembl Mouse
Match: Ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:MGI Symbol;Acc:MGI:107940]) HSP 1 Score: 242.662 bits (618), Expect = 1.033e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 560 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 705 HSP 2 Score: 129.413 bits (324), Expect = 2.051e-30 Identity = 74/203 (36.45%), Postives = 100/203 (49.26%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E +M VL+ + ++CA+++L+ +K+C QVY + K+ + + D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 381 MKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVPTEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 550 HSP 3 Score: 99.7525 bits (247), Expect = 5.514e-21 Identity = 74/244 (30.33%), Postives = 117/244 (47.95%), Query Frame = 2
Query: 341 IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
I+ + +M S + L+ VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L DN+ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 65 IQPVHIMTSVSSLRGTREVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQKDLEDNRDDKETCPPRKFPA-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 287
BLAST of EZH vs. Ensembl Mouse
Match: Ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:MGI Symbol;Acc:MGI:107940]) HSP 1 Score: 243.047 bits (619), Expect = 1.367e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 595 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 740 HSP 2 Score: 129.798 bits (325), Expect = 2.003e-30 Identity = 74/203 (36.45%), Postives = 100/203 (49.26%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E +M VL+ + ++CA+++L+ +K+C QVY + K+ + + D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 416 MKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVPTEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 585 HSP 3 Score: 117.472 bits (293), Expect = 1.338e-26 Identity = 95/333 (28.53%), Postives = 155/333 (46.55%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMH-----PYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ ++ K + + P + S + + DF ++ ++ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L DN+ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +H + ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKTMFSSNRQKILERTETLNQEWKQRRIQPV--HIMTSCSVTSDLDFPAQVIP------LKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQKDLEDNRDDKETCPPRKFPA-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHRKCSYSFHATPNTYKRKNTETALDNKPCGPQCYQ 322
BLAST of EZH vs. Ensembl Mouse
Match: Ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:MGI Symbol;Acc:MGI:107940]) HSP 1 Score: 243.047 bits (619), Expect = 1.448e-68 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 599 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 744 HSP 2 Score: 129.798 bits (325), Expect = 1.689e-30 Identity = 74/203 (36.45%), Postives = 100/203 (49.26%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E +M VL+ + ++CA+++L+ +K+C QVY + K+ + + D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 420 MKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVPTEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 589 HSP 3 Score: 124.79 bits (312), Expect = 6.661e-29 Identity = 97/333 (29.13%), Postives = 155/333 (46.55%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ ++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L DN+ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKTMFSSNRQKILERTETLNQEWKQRRIQPVHIMTSVSSLRGTRECSVTSDLDFPAQVIPLKT----LNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQKDLEDNRDDKETCPPRKFPA-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 326
BLAST of EZH vs. Ensembl Mouse
Match: Ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:MGI Symbol;Acc:MGI:1097695]) HSP 1 Score: 242.276 bits (617), Expect = 2.947e-68 Identity = 110/147 (74.83%), Postives = 127/147 (86.39%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIET 2326
++CKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGE+ISQDEADRRGK+YDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+AG+ELFFDYRY + LKYVGIERE +
Sbjct: 600 VSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDV 746 HSP 2 Score: 122.479 bits (306), Expect = 4.017e-28 Identity = 69/190 (36.32%), Postives = 99/190 (52.11%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+WT +E ++ V + ++C++++LL +K+C QV+ + KE L + T + +N ++KK K R + + + K N++ +V + Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 433 EWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAV---KESLILKLP-----TDELMN---PAQKKKRKHRL-----WAAHCRKIQLKKDNNST---QVYN-------------YQPCDHPDRPCDSTCPCIMTQNFCEKFCQCSPDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCG 590 HSP 3 Score: 119.783 bits (299), Expect = 3.076e-27 Identity = 92/305 (30.16%), Postives = 140/305 (45.90%), Query Frame = 2
Query: 197 QIAMVSKMKSLS--PFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGED---HDPKFIEELLRNYEGRVHGN---FPFD--FNENQLIDLLKTLNEKYDWYKLADN------------------KVKKHQ----EKIENFDYP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKL 1012
++ V MK +S PF +K + D S +R L + +P+M SW+PLQQNF VEDE L NIPYMG++ D FIEEL+ NY+G+VHG P ++ ++L+ LN+ D + N K K+H +K +P ++IF AIA F + + RY E+ +S+ +A TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ +++ N PC +CF L
Sbjct: 64 RVQPVQPMKPVSGHPFLKKCTIESIFPGFD---------SQDMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEDGHNDPSDGKQDDSKEDLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALP--PQCTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLL 340
BLAST of EZH vs. UniProt/SwissProt
Match: sp|P42124|EZ_DROME (Histone-lysine N-methyltransferase E(z) OS=Drosophila melanogaster OX=7227 GN=E(z) PE=1 SV=2) HSP 1 Score: 250.751 bits (639), Expect = 1.906e-70 Identity = 114/146 (78.08%), Postives = 128/146 (87.67%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
ITCKN+ +QRG KHLLM+ SD+AGWGI+ KE KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHS+NPNC AK+++V GDHRIGIFAK I+ G+ELFFDYRYGPTEQLK+VGIERE+E
Sbjct: 613 ITCKNVCVQRGLHKHLLMAPSDIAGWGIFLKEGAQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSINPNCYAKVMMVTGDHRIGIFAKRAIQPGEELFFDYRYGPTEQLKFVGIEREME 758 HSP 2 Score: 182.57 bits (462), Expect = 1.179e-46 Identity = 174/663 (26.24%), Postives = 260/663 (39.22%), Query Frame = 2
Query: 62 NKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMV---SKMKSLSPF------CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE-----------------------DHDPKFIEE---------LLRNYEGRVHGNFPFDFNENQLIDLLKTLN-------------------EKYDW---------------YKLADNKVKKHQEKIEN-FDYP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSS--DVIGPCEKNCFKL---ELTNIEGDSLNENINHC------------------DYNQ----------------------------------WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYH--PCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
N ++ + VK EY ++ Q K+ D++++ N D + + + SK+ P+ C K +Y+ I S K + ++ + +P M +WAP QQNF VEDE L NIPYMG+ D DP F+++ L+R+Y + P + L K+ EK D + D VK E++++ +P IIF+AI+ F D +L +Y+E L+E + + TPN+D ++A S+ R+ H+F +L CRRCFKYDC +H + Y PC +C+ L + DS I+ C D+N WT +++ + VL ++ +YCA++ + +K+C QVY + Q KED E + D + T + K++ R + +L KD + SN Y+ PCDHPG PCD C+C Q NFCEKFC CS C NRF GC+C+ CNTKQCPC+LA RECD DLCQ C
Sbjct: 2 NSTKVPPEWKRRVKSEYIKIRQQKRYKRADEIKEAWIRNWDEHNHNVQDLYCESKVWQAKPYDPPHVDCVKRAEVTSYN-------GIPSGPQKVPICVINAVTPIPTMYTWAPTQQNFMVEDETVLHNIPYMGDEVLDKDGKFIEELIKNYDGKVHGDKDPSFMDDAIFVELVHALMRSYSKELEEAAPGTATAIKTETLAKSKQGEDDGVVDVDADGESPMKLEKTDSKGDLTEVEKKETEEPLETEDADVKPDVEEVKDKLPFPAPIIFQAISANFPDKGTAQELKEKYIE---LTEHQDPERPQECTPNIDG----------IKA----ESV---SRERTMHSFHTLFCRRCFKYDCFLHRLQGHAGPNLQKRRYPELKPFAEPCSNSCYMLIDGMKEKLAADSKTPPIDSCNEASSEDSNDSNSQFSNKDFNHENSKDNGLTVNSAAVAEINSIMAGMMNITSTQCVWTGADQALYRVLHKVYLKNYCAIAHNMLTKTCRQVYEFAQ---KEDAE--FSFEDLRQDFTPPRKKKKKQRLWSLHCRKI----QLKKD------------------------SSSNHVYNYTPCDHPGHPCDMNCSCIQTQNFCEKFCNCSSDCQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQACG 604
BLAST of EZH vs. UniProt/SwissProt
Match: sp|Q08BS4|EZH2_DANRE (Histone-lysine N-methyltransferase EZH2 OS=Danio rerio OX=7955 GN=ezh2 PE=2 SV=1) HSP 1 Score: 243.817 bits (621), Expect = 6.375e-68 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ KE + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 613 VSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 758 HSP 2 Score: 132.109 bits (331), Expect = 2.908e-30 Identity = 73/191 (38.22%), Postives = 96/191 (50.26%), Query Frame = 2
Query: 1076 WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + A D T Q ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 446 WSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIARAPAVDENTPQ--------RKKKRKHRLW--------------------ATHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 603 HSP 3 Score: 126.716 bits (317), Expect = 1.284e-28 Identity = 99/355 (27.89%), Postives = 166/355 (46.76%), Query Frame = 2
Query: 53 RTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPY-----IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNE----------------KYDWYKLADNK--VKKHQEKIENFDY---------PEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
R + K +C + VK EY L+ ++ D+V+ + N I E+ ++++ L + S E ++S + +++P + +PVM SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ LN+ K++ L D K + H+E++ + + + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP++++ + + N + PC C+
Sbjct: 6 RKSEKGPVC--WRRRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTDILNQEWKLRRIQPVHIMTPVSSLRGTRECTVDSGFSEFSRQVIPLKTLNAVASVPVMYSWSPLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALNQYSDNEEDDEEDDHHDYKFEKMDLCDGKDDAEDHKEQLSSESHNNDGSKKFPSDKIFEAISSMFPDKGSTEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFQATPNTYKRKNMENLVDSKPCGIYCY 338
BLAST of EZH vs. UniProt/SwissProt
Match: sp|Q61188|EZH2_MOUSE (Histone-lysine N-methyltransferase EZH2 OS=Mus musculus OX=10090 GN=Ezh2 PE=1 SV=2) HSP 1 Score: 243.047 bits (619), Expect = 1.013e-67 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 599 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 744 HSP 2 Score: 129.798 bits (325), Expect = 1.181e-29 Identity = 74/203 (36.45%), Postives = 100/203 (49.26%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E +M VL+ + ++CA+++L+ +K+C QVY + K+ + + D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 420 MKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVPTEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 589 HSP 3 Score: 124.79 bits (312), Expect = 4.659e-28 Identity = 97/333 (29.13%), Postives = 155/333 (46.55%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ ++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L DN+ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKTMFSSNRQKILERTETLNQEWKQRRIQPVHIMTSVSSLRGTRECSVTSDLDFPAQVIPLKT----LNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQKDLEDNRDDKETCPPRKFPA-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQ 326
BLAST of EZH vs. UniProt/SwissProt
Match: sp|Q28D84|EZH2_XENTR (Histone-lysine N-methyltransferase EZH2 OS=Xenopus tropicalis OX=8364 GN=ezh2 PE=2 SV=1) HSP 1 Score: 243.047 bits (619), Expect = 1.058e-67 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 601 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 746 HSP 2 Score: 130.568 bits (327), Expect = 7.600e-30 Identity = 74/203 (36.45%), Postives = 101/203 (49.75%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 422 MKPNIEPPENVEWSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPVIAEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 591 HSP 3 Score: 126.331 bits (316), Expect = 1.902e-28 Identity = 95/336 (28.27%), Postives = 158/336 (47.02%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLA------------------DNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKL 1012
VK EY L+ ++ D+V+ + N I E+ +++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + D+ DN + + + F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N +++ PC +C++L
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKSMFNTNRQKIMERTEILNQEWKQRRIQPVHIMTTVSSLRGTRECSVTSDLDFPKQVIPLKT----LTAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALAQYSDYEDDEDGDDNQDDERDDTAKDQDDNMEDQETQPLRKFPS-DKIFEAISSMFPDKGTLEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNNEAANDGKPCGPHCYQL 329
BLAST of EZH vs. UniProt/SwissProt
Match: sp|Q4R381|EZH2_MACFA (Histone-lysine N-methyltransferase EZH2 OS=Macaca fascicularis OX=9541 GN=EZH2 PE=2 SV=1) HSP 1 Score: 243.047 bits (619), Expect = 1.064e-67 Identity = 110/146 (75.34%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ K+ + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 599 VSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 744 HSP 2 Score: 130.954 bits (328), Expect = 5.217e-30 Identity = 75/203 (36.95%), Postives = 100/203 (49.26%), Query Frame = 2
Query: 1040 LNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+ NI + +W+ +E +M VL+ + ++CA+++L+ +K+C QVY + K+ + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 420 MKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIAPAPAEDVDTPP--------RKKKRKHRLW--------------------AAHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 589 HSP 3 Score: 125.176 bits (313), Expect = 3.911e-28 Identity = 96/333 (28.83%), Postives = 156/333 (46.85%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPF-----CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLN---------------EKYDWYK-LADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFK 1009
VK EY L+ ++ D+V+ + N I E+ +++ K + + P +R ++ S D + K+ L + +P+M SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L E+ + K L D++ K F + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N ++ PC C++
Sbjct: 19 VKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKT----LNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPS-DKIFEAISSMFPDKGTAEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNAYKRKNTETALDNKPCGPQCYQ 326
BLAST of EZH vs. TrEMBL
Match: H9CXT4 (EZH OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 1575.45 bits (4078), Expect = 0.000e+0 Identity = 775/777 (99.74%), Postives = 776/777 (99.87%), Query Frame = 2
Query: 41 MTKHRTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLXXXXXXXXXXXXXXXXXITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN 2371
MTKHRTANKIEIC+NFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLN NINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLSNSNNNNNGTDSNTNNSITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN
Sbjct: 1 MTKHRTANKIEICDNFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNVNINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLSNSNNNNNGTDSNTNNSITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN 777
BLAST of EZH vs. TrEMBL
Match: A0A5B9BW22 (Enhancer of zeste-like protein OS=Dugesia japonica OX=6161 GN=ezh PE=2 SV=1) HSP 1 Score: 1350.88 bits (3495), Expect = 0.000e+0 Identity = 656/780 (84.10%), Postives = 718/780 (92.05%), Query Frame = 2
Query: 38 PMTKHRTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDY-KNGNDNLSDC-EVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLXXXXXXXXXXXXXXXXXITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN 2371
PM K +TA+KIEI E+FVEIVKLEYNSLQDQIILKKKDDVRQI+K N ++IDEQ +VSKMKSL+PFCEKVRFSQTYSEHDDFEVKINSKS+KSRVRL PYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQL+DLLK LNEKY+W K++ K+KK+ +ENFD+PEIIFK IAEAFGD EDY KLIARY+EMK ++EK+A+DGV+TVTPNLDNEEE+REMEKFLR TQP+N++IEHKR+DATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNAN Y SD+ GPCEKNCFK+E NI + N N +CD NQWTLSERTMLNVLLP+FR DYCALSQL+KSK+C+QV+ YVQ +KKEDLEN+MT ++NQ HQTV NNRGIKRKKMKQRKRPLPKISK+ D DY KNGNDNLSDC EVI+HSAFGSKTDSNQFYHPCDHPGFPCDE+CNCRQ GNFCEKFCQCSR+CSNRFIGCKCRG CNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTN + NG++TK +SLSNSGAENSHDSF SH STNG+YKTQ+SN+S Q++ ++++NN + N+ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDI+KNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQN+SGNQSSNRKYSN
Sbjct: 6 PMNKLKTAHKIEISEDFVEIVKLEYNSLQDQIILKKKDDVRQIMKQNFEVIDEQHEIVSKMKSLTPFCEKVRFSQTYSEHDDFEVKINSKSFKSRVRLFPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLMDLLKILNEKYNWDKVSSGKIKKNVGTVENFDFPEIIFKVIAEAFGDAEDYEKLIARYVEMKSMAEKNATDGVDTVTPNLDNEEEMREMEKFLRTTQPDNTLIEHKREDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANVYLSDIAGPCEKNCFKIEFPNINEELHNGNELNCDLNQWTLSERTMLNVLLPIFRIDYCALSQLIKSKTCSQVFLYVQTFKKEDLENLMTTNENQNHQTVTNNRGIKRKKMKQRKRPLPKISKIINDPDYLKNGNDNLSDCGEVIEHSAFGSKTDSNQFYHPCDHPGFPCDERCNCRQAGNFCEKFCQCSRTCSNRFIGCKCRGQCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNFESNGVSTKSTSLSNSGAENSHDSFNSHIFSTNGVYKTQISNESIQREF-SNSSNNCVDSTANPNNITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDILKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNISGNQSSNRKYSN 784
BLAST of EZH vs. TrEMBL
Match: A0A0R3WMM7 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS2002 PE=4 SV=1) HSP 1 Score: 428.713 bits (1101), Expect = 9.838e-133 Identity = 249/621 (40.10%), Postives = 330/621 (53.14%), Query Frame = 2
Query: 644 FKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVT-PNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNN---ANAYSSDVIGPCEKNCFK-LELTNIEGDSLNENINHCDYN-QWTLSERTMLNVLLPLF----------RTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISK-----LAKDLDYKNGNDNL----------------SDCEVIDHS----AFGSKTDSN------------------QFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSY--SLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLXXXXXXXXXXXXXXXXXITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
FKAIA FG VED KL Y EMK S T + PNLD+ E++E+ E R DA HTFR+L CRRCFKYDCA+HPY+S+++MW++ +++ + C NC + L E + D +W +SE+T+ VL P++ C LS +K+++C +V Y ++ +D + Q+ N + ++ KR + ++ D D N +L S ++ H FGS +++ ++ PCDHPG C+E C+C++ GNFCEKFCQC CSNRF GC+CRG CN++ CPC LA RECD DLC C + + ++ N+D NG ++ S ++H+ ITC+N+AIQRG RKHLLM+ SDVAGWGI+ K+ KNEFIYEYCGEIISQDEA+RRGKIYD+TM SFLFNLN+ F VDA RKGNKIRFANHS+NPNC AK+L+VNGDHRIGIFAK ++ G+ELFFDYRYGPT QLKYVGIER+ E
Sbjct: 331 FKAIALTFGSVEDSNKLQVHYCEMKNAGNNSVPSESGTPSCPNLDDPIEVKEV--------CEREQKRPSRHDALHTFRALFCRRCFKYDCALHPYKSTQTMWSHRWPVKSWACSNLPLCGPNCVRALRSAGTENAGKECKVKEGDKEPEWEISEQTLFEVLAPIYVPFGQPSFHNHNWCCVLSGFIKTRTCKEVLRYCESKISKDPFLLFGHDAGQSRLVNQYNDEVSIPSLRNGKRFFGDANSDFDDLISDDEDGVGANGDLVGFLVGGKKKRRKAKRSRTSLVKHPFASRRFGSVNETSDPPPGHMGKTELTTGCLSNYFQPCDHPGSRCNESCSCKKAGNFCEKFCQCPVDCSNRFTGCRCRGQCNSRVCPCVLAIRECDPDLCHSCGAGTSRQPVVATHETALNVDDNGNSS---------------SGLTHA-------------------------------------ITCRNVAIQRGLRKHLLMASSDVAGWGIFMKDGAEKNEFIYEYCGEIISQDEAERRGKIYDRTMCSFLFNLNRQFAVDAMRKGNKIRFANHSINPNCYAKVLMVNGDHRIGIFAKRAVQPGEELFFDYRYGPTHQLKYVGIERDDE 891 HSP 2 Score: 122.479 bits (306), Expect = 1.268e-24 Identity = 71/199 (35.68%), Postives = 111/199 (55.78%), Query Frame = 2
Query: 44 TKHRTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQI--AMVSKMKSLSP-----------FCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGED--HDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKV 595
T+ R+ N+ I +N + + Y ++ + +L K + V + + N +ID+Q+ A K+LS V Q YS+ FE KIN + V ++P ++ +P M SWAP+Q N+SVEDE L ++PYMGE+ D F+EELL NY+GR+HGNFPFDF + ++L+ T++ K W L + +V
Sbjct: 44 TRQRSDNRKRILQNAIRDI---YQFIRKRHLLAKWNRVGVLYRRNDALIDKQLKEAANRNWKNLSVSINGVSSNALLLAAPVEIPQLYSQLGRFEAKINGYVARVGVSIIPPVDPVPAMSSWAPVQLNYSVEDEPVLNHLPYMGEEQAQDDVFLEELLNNYDGRLHGNFPFDFESHLTVELVDTVHSK--WATLPNTEV 237
BLAST of EZH vs. TrEMBL
Match: A0A094ZVS5 (Histone-lysine N-methyltransferase E(Z) OS=Schistosoma haematobium OX=6185 GN=MS3_07415 PE=4 SV=1) HSP 1 Score: 417.927 bits (1073), Expect = 1.443e-124 Identity = 264/623 (42.38%), Postives = 323/623 (51.85%), Query Frame = 2
Query: 608 EKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSA-SDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSM----WNNANAYSSDVIGP-CEKNCFKLELTNIEGDSLNENINH--CDYNQWTLSERTMLN---VLLPLFRTDYCALSQLL----------KSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPL-------------PKISKLAKDLDYK---------NGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFIS-HSVSTNGLYKTQVSNDSFQKDLXXXXXXXXXXXXXXXXXITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGN 2344
+++ P+ +F AIA FG +D KL RY+E+K S S ++ T PNLD+ E+ K R P R +A H+FR+L CRRCFKYDCA+HPY+S++SM W + + + P C C + N IN CD+N LS N +LLP D LSQL +S S A + + + + VNNN + +R P +L D D + NGN N V+ F YHPCDHPG CD+ C+CR G FCEKFCQC C NRF+GC+CRG CNTK CPC LA RECD DLC C GA +S SF S +S+ L +T + + TC+N+AIQRG RKHLLM+ SDVAGWGI+ KE KN+FIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLN+ FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I G+ELFFDYRYGPTEQLKYVGIER+ + ++ N
Sbjct: 828 KRLNTTGIPDGVFSAIAGTFGSSDDSSKLQLRYMELKERSSNSQLNEEALTCCPNLDSPIEVLNAVKSGRHQIP-------TRAEALHSFRTLFCRRCFKYDCALHPYKSTQSMWSHRWPFVASTNVESDAPYCGVWCVRQFTDKQNNHHHNNRINERDCDWNP-VLSYANTQNHSTLLLP----DSGQLSQLRVPGTGIEWGRRSTSTASLNDGGDCAGSGSCSGSVQDNGEFNEEAVNNNESSGYNLLPSYRRKRSRKRRLKKSMLLGPPTRRLDDDEDSQTNGCHQNATNGNAN----GVVASGCFA------HHYHPCDHPGQRCDDSCSCRIAGTFCEKFCQCPPDCPNRFLGCRCRGQCNTKLCPCVLAVRECDPDLCLSC--------------------------------GAHSSFRSFASGNSMDLLSLLQTTLP----------------------PVTGTCRNVAIQRGWRKHLLMAPSDVAGWGIFIKEAAEKNDFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNRDFVVDATRKGNKIRFANHSVNPNCHAKVIMVNGDHRIGIFAKRAILPGEELFFDYRYGPTEQLKYVGIERDTDAASVINN 1374 HSP 2 Score: 116.701 bits (291), Expect = 1.161e-22 Identity = 59/178 (33.15%), Postives = 98/178 (55.06%), Query Frame = 2
Query: 95 IVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQI--AMVSKMKSLSPFC----------------EKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDH---DPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKY 565
+++ YN ++ + K + V +++ N ++ EQ+ A + + + S C E V YS+ FE +I SY+ + ++P ++ +P + WAP+Q NFSVEDE EL N+PYMG+D D F+EELL NY+G +HGNFPF+F E+ L+ L+ + + +
Sbjct: 422 MIREVYNRIRCANLTSKWNHVFELMSKNRRLLSEQLNSAHAANLAARSITCDEMWPNSNSADPPLLAEPVEIPNVYSQFGKFEARIGGVSYRVAITMMPQVDPVPSVCMWAPVQHNFSVEDETELANLPYMGDDQAQEDVNFLEELLNNYDGHLHGNFPFEFEESLLVQLVNAVAKAW 599
BLAST of EZH vs. TrEMBL
Match: A0A074Z192 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_11922 PE=4 SV=1) HSP 1 Score: 400.593 bits (1028), Expect = 5.955e-118 Identity = 249/647 (38.49%), Postives = 330/647 (51.00%), Query Frame = 2
Query: 632 PEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKS-ASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNN----ANAYSSDVIGP-CEKNCFKLELTNIEGDSLNENINHCDYNQ--WTLSERTMLNVLLPLFR----------TDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPL-----PKISKLAKDLDYKNGN---DNLSDCEVIDHSAFGS---------------------KTDSNQFYH-------------PCDHPGFPCDEKC------NCRQVGNFCEKFC-------------QCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLXXXXXXXXXXXXXXXXXITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNL 2335
P+ +F AIA FG ED KL RY E++ + S ++ + +PNLDN E+ + K R+ P R +A H+FR+L CRRCFKYDCA+HPY+S++SMW++ + + + P C + C + L + DS N +Q WT E +++ VL P+F T C L+++L +K+C V +Y+ + L N Q Q GI+ + L P ++ +NG+ D+ D + +S F S K D + H ++ G C C G C+++C QC C+NRF+GC+CRG CNTK CPC LA RECD DLC C + R++ ++ T LD + + SS +N+G TC+N+AIQRG RKHLLM+ SDVAGWGI+ K+ KN+FIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLN+ FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I G+ELFFDYRYGPTEQLKYVGIER+ + +
Sbjct: 812 PDAVFTAIAGTFGSAEDANKLQHRYSELRDRANSSQTAEEPLSCSPNLDNLLEVALVMKNGRSCPP-------SRSEALHSFRALFCRRCFKYDCALHPYKSTQSMWSHRWPITHTTNLEPDAPYCGRWCVRQCLNSSTDDSQAIVQNPAKISQCVWTPVEISLIQVLAPVFMPVGYSSYPQYTWCCTLARILGTKTCQDVLNYINSRPTSGLTN---PDSGQIAQLRVPGVGIEWTRRSASTASLNDVADPSLNGSCSGSILENGDPTEDSTIDSATVAYSHFSSRRKRWKKRRLKKTMLPGPLVRKLDEDDESHMNSNGTSGGGNSSAANNAGGLLASGCFAHQYHPCDHPGQRCDEYCPCRQAGTFCEKFCQCPAECTNRFLGCRCRGQCNTKLCPCSLAVRECDPDLCLSCGAQQPGRTFG-QNGTTLDNSMMICPVSSSANTG--------------------------------------------------TCRNVAIQRGWRKHLLMAPSDVAGWGIFIKDGAEKNDFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNRDFVVDATRKGNKIRFANHSVNPNCHAKVMMVNGDHRIGIFAKRAIAPGEELFFDYRYGPTEQLKYVGIERDADASTI 1397 HSP 2 Score: 129.413 bits (324), Expect = 1.260e-26 Identity = 66/180 (36.67%), Postives = 105/180 (58.33%), Query Frame = 2
Query: 110 YNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMV-------------SKMKSLS----------PF--CEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDH---DPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKY 565
YN ++ +++ K VR ++ +N ++ EQ+A+V S SL P+ E V Q Y++ FE KI +K+Y+ + ++P ++ +P + +WAP+Q NFSVEDE EL N+PYMG++ D F+EELL NY+GR+HGNFPF+F E LI L+ +N+++
Sbjct: 466 YNGIRRNVLVSKWSQVRTLMTYNRRLLTEQLAVVKAANLAASSLASDSFFDSLDQVNGDPSGAYPYLLAEPVEIPQIYNQFGRFEAKIGNKTYRVGLIMIPQVDSVPNVCTWAPVQHNFSVEDETELANLPYMGDEQAQEDVSFLEELLNNYDGRLHGNFPFEFEEEMLIPLVTEVNKQW 645
BLAST of EZH vs. Ensembl Cavefish
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:103027230]) HSP 1 Score: 243.432 bits (620), Expect = 1.700e-69 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ KE + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 512 VSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 657 HSP 2 Score: 103.605 bits (257), Expect = 2.322e-22 Identity = 75/241 (31.12%), Postives = 120/241 (49.79%), Query Frame = 2
Query: 332 LPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFD----------YP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
L I+ + +M + L+ VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ LN+ Y + D+ +H K+E D +P + IF+AI+ F D +L +Y E L+E+ + TPN+D P ++ R+ + H+F +L CRRCFKYDC +HP+ ++ + + N + PC +C+
Sbjct: 62 LRRIQPVHIMTPVSSLRGTREVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALNQ-YSDNEDDDDDDDQHDYKLEKMDVCRSSDCSKKFPSDKIFEAISSMFPDKGSPEELKEKYKE---LTEQQLPGALPPECTPNIDG---------------PNAKSVQ--REQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNMENMVDSKPCGIDCY 281 HSP 3 Score: 63.1586 bits (152), Expect = 9.890e-10 Identity = 24/35 (68.57%), Postives = 27/35 (77.14%), Query Frame = 2
Query: 1538 NRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 468 NRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 502
BLAST of EZH vs. Ensembl Cavefish
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:103027230]) HSP 1 Score: 243.432 bits (620), Expect = 9.660e-69 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ KE + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 615 VSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 760 HSP 2 Score: 130.183 bits (326), Expect = 1.205e-30 Identity = 72/191 (37.70%), Postives = 95/191 (49.74%), Query Frame = 2
Query: 1076 WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 448 WSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESSIIARAPAEDEDTPP--------RKKKRKHRLW--------------------ATHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 605 HSP 3 Score: 124.405 bits (311), Expect = 9.105e-29 Identity = 94/331 (28.40%), Postives = 158/331 (47.73%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPY-----IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGK--LIARYLEMKLLSEKSASD----GVETVTPNLDNEEEIREMEKFLRATQPENSIIEH-------------KRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
VK Y L+ ++ D+V+ + N I E+ ++++ L + S E ++S + +++P + +PVM SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ LN+ Y + D+ +H K+E D + + D +D K L+ S+K SD + ++ P+ + EE++E K L Q ++ +R+ + H+F +L CRRCFKYDC +HP+ ++ + + N + PC +C+
Sbjct: 19 VKSVYMRLRQLKRFRRADEVKSMFNSNRQKILERTDILNQEWKLRRIQPVHIMTPVSSLRGTRECTVDSGFTEFSKQVIPLKTLNAVASVPVMYSWSPLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALNQ-YSDNEDDDDDDDQHDYKLEKMD--------VCDGKDDTDDSRKDQLLNSESRSSDCSKKFPSDKIFEAISSMFPDKGSPEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNMENMVDSKPCGIDCY 340
BLAST of EZH vs. Ensembl Cavefish
Match: EZH1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:HGNC Symbol;Acc:HGNC:3526]) HSP 1 Score: 241.891 bits (616), Expect = 3.064e-68 Identity = 110/147 (74.83%), Postives = 127/147 (86.39%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIET 2326
++CKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGEIISQDEADRRG+IYDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE++
Sbjct: 599 VSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKEPVQKNEFISEYCGEIISQDEADRRGRIYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQQGEELFFDYRYSQADALKYVGIEREVDV 745 HSP 2 Score: 132.88 bits (333), Expect = 1.396e-31 Identity = 71/192 (36.98%), Postives = 99/192 (51.56%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
QW+ ++ ++ VL + ++C++++L+ +K+C QVY + KE L H+ + GI +K K++ R +S + +D+S SNQ Y PCDHP PCD C C NFCEKFCQC C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 434 QWSGADESLFRVLHGTYYNNFCSIARLIGTKTCKQVYEFAM---KEVL----------IHRVPLEDAGISPQKKKRKHRFTVVLSAIV----------------ALDNS-------SNQVYNYQPCDHPEHPCDSSCPCVMTQNFCEKFCQCDHECQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCMTCG 589 HSP 3 Score: 110.538 bits (275), Expect = 1.714e-24 Identity = 100/353 (28.33%), Postives = 163/353 (46.18%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDV-------RQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSR---VRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLA--------------------DNKV------KKHQEKIENFDYP-------EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLE 1015
VK EY L+ +K ++V RQ ++ D+++E+ + + +++S+ PF + S + V+ S+ ++ +R L + +P M SW+PLQQNF VEDE L NIPYMG+ + D F+EEL+ NY+G VHG+ F N+ +L++ L++ D + +NK +K + E + P + IF AIA F +L +Y + LL S+ TPN+D F ++ Q E S+ H+F +L CRRCFKYDC +HP+ +S +++ + PC +CF L+
Sbjct: 26 VKSEYMRLRQLKRFRKAEEVKALFISNRQKIELRTDLLNEEWSKL-RIQSI-PFSTQ---SGSMPSKKQCSVEFGFPSFPNQAVAMRPLTTVAGIPFMYSWSPLQQNFMVEDETFLHNIPYMGDEVLEQDEAFLEELIDNYDG-VHGDREGGFINDEIFKELVEALSQYSDQEEEEESTEEEEERGVRRSAGENTEENKAVSLAFKRKRRRVTEGSELPTSKKIPHDKIFTAIASMFPYKGTTEELKEKYKD--LLEPPSSVKLPPLCTPNMDG--------PFAKSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFHASPNVYKRKSKEIRMETEPCGIDCFLLQ 353
BLAST of EZH vs. Ensembl Cavefish
Match: EZH1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:HGNC Symbol;Acc:HGNC:3526]) HSP 1 Score: 241.891 bits (616), Expect = 3.306e-68 Identity = 110/147 (74.83%), Postives = 127/147 (86.39%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIET 2326
++CKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGEIISQDEADRRG+IYDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE++
Sbjct: 603 VSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKEPVQKNEFISEYCGEIISQDEADRRGRIYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQQGEELFFDYRYSQADALKYVGIEREVDV 749 HSP 2 Score: 135.191 bits (339), Expect = 2.905e-32 Identity = 73/192 (38.02%), Postives = 98/192 (51.04%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
QW+ ++ ++ VL + ++C++++L+ +K+C QVY + KE L H+ + GI +K K++ R KI +L KD SNQ Y PCDHP PCD C C NFCEKFCQC C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 440 QWSGADESLFRVLHGTYYNNFCSIARLIGTKTCKQVYEFAM---KEVL----------IHRVPLEDAGISPQKKKRKHRLWAKI-QLKKD------------------------NSSNQVYNYQPCDHPEHPCDSSCPCVMTQNFCEKFCQCDHECQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCMTCG 593 HSP 3 Score: 110.538 bits (275), Expect = 1.854e-24 Identity = 100/353 (28.33%), Postives = 163/353 (46.18%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDV-------RQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSR---VRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNEKYDWYKLA--------------------DNKV------KKHQEKIENFDYP-------EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLE 1015
VK EY L+ +K ++V RQ ++ D+++E+ + + +++S+ PF + S + V+ S+ ++ +R L + +P M SW+PLQQNF VEDE L NIPYMG+ + D F+EEL+ NY+G VHG+ F N+ +L++ L++ D + +NK +K + E + P + IF AIA F +L +Y + LL S+ TPN+D F ++ Q E S+ H+F +L CRRCFKYDC +HP+ +S +++ + PC +CF L+
Sbjct: 32 VKSEYMRLRQLKRFRKAEEVKALFISNRQKIELRTDLLNEEWSKL-RIQSI-PFSTQ---SGSMPSKKQCSVEFGFPSFPNQAVAMRPLTTVAGIPFMYSWSPLQQNFMVEDETFLHNIPYMGDEVLEQDEAFLEELIDNYDG-VHGDREGGFINDEIFKELVEALSQYSDQEEEEESTEEEEERGVRRSAGENTEENKAVSLAFKRKRRRVTEGSELPTSKKIPHDKIFTAIASMFPYKGTTEELKEKYKD--LLEPPSSVKLPPLCTPNMDG--------PFAKSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFHASPNVYKRKSKEIRMETEPCGIDCFLLQ 359
BLAST of EZH vs. Ensembl Cavefish
Match: suv39h1b (suppressor of variegation 3-9 homolog 1b [Source:ZFIN;Acc:ZDB-GENE-030131-5105]) HSP 1 Score: 104.76 bits (260), Expect = 1.153e-23 Identity = 58/135 (42.96%), Postives = 80/135 (59.26%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVA-GWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNL---NQSFVVDATRKGNKIRFANHSVNPNC-CAKILLVNGDH---RIGIFAKHNIKAGDELFFDYR 2272
C N +Q+G + L + +D GWG+ T E I KN F+ EY GEII+ +EA+RRG +YDK +++LF+L + + VDA GN F NHS +PN + + N D RI FA IKAG+EL FDY+
Sbjct: 239 CPNRVVQKGIQYSLCIFRTDNGRGWGVRTMERIRKNTFVMEYVGEIITTEEAERRGHVYDKEGTTYLFDLDYVDDEYTVDAAHYGNISHFVNHSCDPNLQVYNVFIENLDERLPRIAFFATRPIKAGEELTFDYK 373
BLAST of EZH vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004922.1 (pep scaffold:Pmarinus_7.0:GL479351:23010:32607:-1 gene:ENSPMAG00000004401.1 transcript:ENSPMAT00000004922.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 237.654 bits (605), Expect = 2.946e-67 Identity = 109/146 (74.66%), Postives = 125/146 (85.62%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN IQRG +KHLL++ SDVAGWGI+ K + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSV+PNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 636 VSCKNCCIQRGLKKHLLLAPSDVAGWGIFIKVPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVSPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 781 HSP 2 Score: 134.806 bits (338), Expect = 7.945e-33 Identity = 79/206 (38.35%), Postives = 105/206 (50.97%), Query Frame = 2
Query: 1064 DYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCC-APKHI-NRSYSLK 1675
D +WT +E ++ VL F ++CA+++L+++KSC QVY + A K+ + + D T RKK ++ + K+ D S ++ Y PCDHPG CD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLCQ C A H NR+ S K
Sbjct: 465 DPVEWTGAEESLFRVLHGTFFNNFCAIARLIETKSCRQVYEF--AVKESSVMPRVPTDDANTP---------PRKKKRKHRLWAAHCRKIQLKKD--------------------SSSNHVYNYQPCDHPGQVCDSSCPCVNAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCQTCGAADHWDNRNVSCK 639 HSP 3 Score: 119.783 bits (299), Expect = 5.265e-28 Identity = 96/353 (27.20%), Postives = 159/353 (45.04%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDV-------RQILKHNLDIIDEQIAM--VSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKS-RVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNE-----------------------------KYDW--YKLADNKV----KKHQEKIENFDYP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
VK EY L+ ++ D+V R +K D+++E+ V ++ ++P + S + + S ++ +R L + +PVM +W+PLQQNF VEDE L NIPYMG+ + D FIEEL++NY+G+VHG+ F N+ ++L+K L + ++ W + + D+ + E +++ +P + IF+AI+ F D +L +Y E+ L + TPN+D R+ Q E S+ H+F +L CRRCFKYDC +HP+ ++ + + N PC CF
Sbjct: 20 VKSEYMRLRQLKRFRRADEVKSAFLANRHKIKDRTDVLNEEWKQRRVQPIQVMTP-----AMPASVSRKCAADCIVGGFSSQTIPLRTLNAVASIPVMYTWSPLQQNFMVEDETVLHNIPYMGDEVLEQDGAFIEELIKNYDGKVHGDRECGFINDEIFVELVKALLQYPDEDDDENEDERMETEEVAGGNVCLCVRRWHWTCFCVLDHGFVLNSQTSSEGLKSKKFPNDKIFEAISNMFPDKGSPDELKEKYRELTELQDPGTLP--PECTPNIDGPN--------ARSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNPEVRMETEPCGVECF 348
BLAST of EZH vs. Ensembl Sea Lamprey
Match: SUV39H2 (suppressor of variegation 3-9 homolog 2 [Source:HGNC Symbol;Acc:HGNC:17287]) HSP 1 Score: 100.138 bits (248), Expect = 1.351e-22 Identity = 55/134 (41.04%), Postives = 76/134 (56.72%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVA-GWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLN---QSFVVDATRKGNKIRFANHSVNPNCCAKILLVN----GDHRIGIFAKHNIKAGDELFFDY 2269
CKN +Q+G L + +D GWG+ T + I KN F+ EY GE+I +EA+RRG IYD+ +++LF+L+ + VDAT GN F NHS PN +N RI FA ++ AG+EL FDY
Sbjct: 242 CKNRVVQKGIPFPLCIFRTDNGRGWGVKTMQKIKKNSFVMEYVGELIPSEEAERRGVIYDRAGTTYLFDLDFCKDDYTVDATFYGNISHFVNHSCEPNLQVYSTFINNHDFSQPRIAFFAMDDLAAGEELTFDY 375
BLAST of EZH vs. Ensembl Sea Lamprey
Match: setd1a (SET domain containing 1A, histone lysine methyltransferase [Source:ZFIN;Acc:ZDB-GENE-080521-4]) HSP 1 Score: 98.2117 bits (243), Expect = 4.036e-21 Identity = 50/128 (39.06%), Postives = 75/128 (58.59%), Query Frame = 2
Query: 1919 QRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYD--KTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLK 2296
++K L + S + WG++ E I E + EY G+ I Q AD R K Y+ SS+LF ++ ++DAT+ GN RF NHS NPNC AK++ V RI I++K I +E+ +DY++ P E+ K
Sbjct: 1713 RKKKLRFTKSRIHEWGLFALEPIAAEEMVIEYVGQGIRQLVADMREKRYEVEGIGSSYLFRVDHDTIIDATKVGNLARFINHSCNPNCYAKVITVESQKRIVIYSKQAINVNEEITYDYKF-PIEENK 1839
BLAST of EZH vs. Ensembl Sea Lamprey
Match: nsd2 (nuclear receptor binding SET domain protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-2581]) HSP 1 Score: 94.3597 bits (233), Expect = 5.564e-20 Identity = 49/128 (38.28%), Postives = 73/128 (57.03%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGK-IYDKTMSSF-LFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDY 2269
C+N A + + + GWG+ T I K EF+ EY GE+I ++E +R K ++K +S+F + + + ++DA KGN RF NHS PNC + VNGD R+G+FA +I G EL F+Y
Sbjct: 1006 CQNQAFTKRAYPPSQIVKTAGRGWGLLTLVSIRKGEFVNEYVGELIDEEECRKRIKYAHEKNLSNFYMLTIEKDRIIDAGPKGNFSRFMNHSCQPNCETQKWTVNGDTRVGLFATSDIATGTELTFNY 1133
BLAST of EZH vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002322.1 (pep scaffold:Pmarinus_7.0:GL477101:2485:64197:1 gene:ENSPMAG00000002099.1 transcript:ENSPMAT00000002322.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 85.8853 bits (211), Expect = 3.526e-17 Identity = 41/117 (35.04%), Postives = 70/117 (59.83%), Query Frame = 2
Query: 1928 HLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSS-FLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRY 2275
++ ++ S + G G+Y DI K+ + EY GEII + A+R K+Y+ T ++F L+ V+DAT G R+ NHS PNC A+++ + +++I I + I+ G+EL +DY +
Sbjct: 3783 NVYLARSRIQGLGLYASRDIEKHTMVIEYIGEIIRNEVANRHEKLYESTNRGVYMFRLDSEHVIDATITGGPARYINHSCAPNCVAELVTFDRENKIIISSCRRIQKGEELTYDYLF 3899
BLAST of EZH vs. Ensembl Yeast
Match: SET1 (Histone methyltransferase, subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3K4; Set1p-dependent H3K4 trimethylation recruits Nrd1p, allowing efficient termination of snoRNAs and cryptic unstable transcripts (CUTs) by Nrd1p-Nab3p-Sen1p pathway; modulates histone acetylation levels in promoter proximal regions to ensure efficient Nrd1p-dependent termination; required in transcriptional silencing near telomeres and at silent mating type loci; has a SET domain [Source:SGD;Acc:S000001161]) HSP 1 Score: 101.679 bits (252), Expect = 2.094e-22 Identity = 51/121 (42.15%), Postives = 75/121 (61.98%), Query Frame = 2
Query: 1919 QRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDK--TMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRY 2275
++K ++ + S + WG+Y + I E I EY GE I Q A+ R K Y K SS+LF ++++ V+DAT+KG RF NH +PNC AKI+ V G RI I+A +I A +EL +DY++
Sbjct: 936 RKKPVMFARSAIHNWGLYALDSIAAKEMIIEYVGERIRQPVAEMREKRYLKNGIGSSYLFRVDENTVIDATKKGGIARFINHCCDPNCTAKIIKVGGRRRIVIYALRDIAASEELTYDYKF 1056
BLAST of EZH vs. Ensembl Yeast
Match: SET2 (Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain(CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000003704]) HSP 1 Score: 85.5001 bits (210), Expect = 1.399e-17 Identity = 52/138 (37.68%), Postives = 75/138 (54.35%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKT-MSSFLFNLNQS-FVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDY---RYGPTEQ 2290
C+N Q+ Q + + + G+G+ ++DI N+FIYEY GE+I + E R YD+ F F + Q+ +DAT KG+ RF NHS +PN +V R+GIFA+ I G+E+ FDY RYG Q
Sbjct: 109 CQNQRFQKKQYAPIAIFKTKHKGYGVRAEQDIEANQFIYEYKGEVIEEMEFRDRLIDYDQRHFKHFYFMMLQNGEFIDATIKGSLARFCNHSCSPNAYVNKWVVKDKLRMGIFAQRKILKGEEITFDYNVDRYGAQAQ 246
BLAST of EZH vs. Ensembl Nematostella
Match: EDO30322 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T142]) HSP 1 Score: 246.128 bits (627), Expect = 1.187e-70 Identity = 108/145 (74.48%), Postives = 131/145 (90.34%), Query Frame = 2
Query: 1889 TCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
TCKN+++QRGQRKH+L++ SDVAGWGIY K+ + KNEFI EYCGE+ISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHS++PNC AK+++VNGDHRIGIFAK +I+AG+ELFFDYRY T+ LK+VGIER+++
Sbjct: 540 TCKNVSLQRGQRKHMLLAPSDVAGWGIYIKQSVKKNEFISEYCGEVISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSISPNCYAKVMMVNGDHRIGIFAKRDIEAGEELFFDYRYSATDALKFVGIERDVD 684 HSP 2 Score: 218.394 bits (555), Expect = 8.969e-61 Identity = 181/636 (28.46%), Postives = 273/636 (42.92%), Query Frame = 2
Query: 86 FVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKS------YKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGED---HDPKFIEELLRNYEGRVHGNFPFD---FNENQLIDLL-KTLNEKYDWYKLADNKVKKHQEKIEN---------FDYPEIIFKAIAEAFGDVEDYGKLI---ARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNI--------------------------------EGDSLNENINHCD-----YNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLP-KISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQ 1804
++ V+LEY L+++ +K+K+ ++ N I EQ+ + C V + + K + S + +R + + +P +W+PLQQNF VEDE L NIPYMGED D FIEEL++NY+G+VH D ++ L++L+ L + K D + K E + + +F+AI + F E KL+ +RY + K E A TPN+D+ PE + R+ + H+F L CRRC+KYDC +H +RS S + PC +C+ L + N+ DS + D ++W+ +E ++L VL ++ +YC +++L+++K+C +VY +A+ E E++ D T +++K K R L + +L KD S + Y PCDHPG PCD+ C C NFCEKFCQC+ C NRF GC+C+ CNTKQCPCFLA RECD DLC C N D + T K SL + H V+ G+Y Q
Sbjct: 5 MIKAVRLEYIRLREKRQMKRKESAKRDFIANRQKIREQLRKRKQKIENVTSCPIVGSALLTEQMQRPARKCTASSGFHHSTQHTLLRTMCAVTPIPNFFTWSPLQQNFVVEDETVLHNIPYMGEDVIEKDNSFIEELIKNYDGKVHNTRMLDDYTLTDDLLVELIDGVLATSSNVAKEPDPQETKGTETKDGQSGATDSQAVVPSDALFEAIIKLFP--EKSWKLVDIKSRYNDAKTRIEGHAP---PECTPNIDS---------------PEAQSVS--REQSLHSFHMLFCRRCYKYDCFLHGWRSFPSQAKRKSPVDLQESSPCGPDCW-LHIKNVAVSPSGSPSKSESSSRKRSKGRKSSQHSQGFPSPDSASPTKKSADELLSQQSEWSGAEASLLRVLRTVYFNNYCTIAKLIETKTCKEVY--FRAFG-ESEESLPVVDDTNTPP--------RKRKRKHRMWSLHCRKIQLKKD----------------------STSTHVYNYIPCDHPGLPCDQSCLCVMTQNFCEKFCQCNSDCQNRFPGCRCKAQCNTKQCPCFLAVRECDPDLCGTCG------------ADNFDQDSKTCKNVSLQR--GQRKHMLLAPSDVAGWGIYIKQ 570
BLAST of EZH vs. Ensembl Nematostella
Match: EDO43763 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXE9]) HSP 1 Score: 103.219 bits (256), Expect = 8.461e-24 Identity = 56/141 (39.72%), Postives = 77/141 (54.61%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYD--KTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDY---RYGPTEQLKY 2299
C N Q G + + + ++ GWG+ T ED+ +N+F+ EYCGE+++ + R + YD K + L ++DAT KG+ RF NHS PNC + VNG RIG F IKAG+EL FDY RYG Q Y
Sbjct: 66 CTNRRFQEGCKIKVEVFKTEKKGWGVKTLEDLEQNQFVIEYCGEVMNYRDFQSRAQRYDRQKRRHYYFMTLRADEIIDATLKGSISRFINHSCEPNCVTQKWTVNGLLRIGFFTLRTIKAGEELTFDYQLQRYGKIAQTCY 206
BLAST of EZH vs. Ensembl Nematostella
Match: EDO44546 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVC2]) HSP 1 Score: 97.8265 bits (242), Expect = 4.308e-23 Identity = 48/130 (36.92%), Postives = 80/130 (61.54%), Query Frame = 2
Query: 1913 RGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDK--TMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLK 2296
R ++K L + S + WG++ E I +E + EY GE+I Q AD R + Y++ SS++F L+++ ++DAT GN RF NH +PNC AK++ V +I I++K +I+ +E+ +DY++ P E K
Sbjct: 70 RVRKKQLKFAKSSIHDWGLFALEPIAADEMVIEYVGEVIRQAIADYRERCYEERGIGSSYMFRLDETTIIDATTMGNFARFINHCCDPNCYAKVIAVENMKKIVIYSKRDIQVDEEITYDYKF-PIEDEK 198
BLAST of EZH vs. Ensembl Nematostella
Match: EDO36231 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ88]) HSP 1 Score: 97.4413 bits (241), Expect = 1.288e-22 Identity = 49/137 (35.77%), Postives = 81/137 (59.12%), Query Frame = 2
Query: 1889 TCKNMAIQRGQRKHL-LMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQ-----SFVVDATRKGNKIRFANHSVNPNCCAKILLVNG----DHRIGIFAKHNIKAGDELFFDY 2269
+C+N +QRG+ + + + GWG+ T + I+KN+F+ EY GE+I+ +EA+ RG+ YD ++LF+L+ ++ +DA + GN F NHS +PN + V+ RI FA+ +I AG+E+ FDY
Sbjct: 106 SCRNRVVQRGRTVRVTIFRTYNGCGWGVKTMDPIMKNQFVTEYVGEVITNEEAEHRGRHYDAAGQTYLFDLDYNDGDCAYTIDAKKYGNISHFINHSCDPNLSVFGVWVDTLDPQMPRIAFFARRDIPAGEEITFDY 242
BLAST of EZH vs. Ensembl Nematostella
Match: EDO30857 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZK5]) HSP 1 Score: 89.7373 bits (221), Expect = 1.314e-19 Identity = 44/118 (37.29%), Postives = 70/118 (59.32%), Query Frame = 2
Query: 1946 SDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYD-KTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLK 2296
S + G G+Y I E + EY G +I D+R Y+ K + ++F ++ ++VVDAT GN RF NHS PNC ++++ ++G+ +I IFA +I G+EL +DY++ P E K
Sbjct: 157 SGIHGRGLYCTRHIAAGEMVIEYSGMLIRSTLTDKREAYYESKGIGCYMFRIDGTYVVDATTSGNAARFINHSCEPNCYSRVVTIDGNKKILIFASKSISRGEELTYDYKF-PLEDEK 273
BLAST of EZH vs. Ensembl Medaka
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:100125823]) HSP 1 Score: 243.047 bits (619), Expect = 1.226e-68 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ KE + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 613 VSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 758 HSP 2 Score: 129.413 bits (324), Expect = 1.910e-30 Identity = 72/192 (37.50%), Postives = 96/192 (50.00%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 445 EWSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESAIIARAPAEDEDTPP--------RKKKRKHRLW--------------------ATHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 603 HSP 3 Score: 123.635 bits (309), Expect = 1.265e-28 Identity = 101/344 (29.36%), Postives = 157/344 (45.64%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS---KMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPY-----IEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNE---------------KYDWYKLADNKVKKH--------QEKIENFD----YP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
VK EY L+ ++ D+V+ + N I E+ +++ K + + P + S E ++S + +++P + +PVM SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + K D L D K E N D +P + IF+AI+ F D +L +Y E L+E+ + TPN+D R+ Q E S+ H+F +L CRRCFKYDC +HP+ ++ + + N + PC +C+
Sbjct: 18 VKSEYMRLRQMKRFRRADEVKGMFNSNRQKIMERTDILNQEWKTRRIQP----IHIMTPVSSRGTRECTVDSGFPEFSRQVIPLKTLNAVASVPVMYSWSPLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALVQYSDNEDDEEDEEQDYKVDKMDLCDGKDHTEDPCKDGHSNESRANSDGSKKFPSDKIFEAISSMFPDKGSLEELKEKYKE---LTEQQLPGALPPECTPNIDGPN--------ARSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNLENLVESKPCGIDCY 337
BLAST of EZH vs. Ensembl Medaka
Match: ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:100125823]) HSP 1 Score: 243.047 bits (619), Expect = 1.622e-68 Identity = 111/146 (76.03%), Postives = 127/146 (86.99%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
++CKN +IQRG +KHLL++ SDVAGWGI+ KE + KNEFI EYCGEIISQDEADRRGK+YDK M SFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I+ G+ELFFDYRY + LKYVGIERE+E
Sbjct: 628 VSCKNCSIQRGAKKHLLLAPSDVAGWGIFIKEPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREME 773 HSP 2 Score: 129.798 bits (325), Expect = 1.816e-30 Identity = 72/192 (37.50%), Postives = 96/192 (50.00%), Query Frame = 2
Query: 1073 QWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQF--YHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
+W+ +E ++ VL+ + ++CA+++L+ +K+C QVY + K+ + A D T ++KK K R + C I GS SN Y PCDHP PCD C C NFCEKFCQCS C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 460 EWSGAEASLFRVLIGTYYDNFCAIARLIGTKTCRQVYEF--RVKESAIIARAPAEDEDTPP--------RKKKRKHRLW--------------------ATHCRKIQLKKDGS---SNHVYNYQPCDHPRQPCDSSCPCVTAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCG 618 HSP 3 Score: 118.242 bits (295), Expect = 7.075e-27 Identity = 86/261 (32.95%), Postives = 125/261 (47.89%), Query Frame = 2
Query: 323 VRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGNFPFDF-NENQLIDLLKTLNE---------------KYDWYKLADNKVKKH--------QEKIENFD----YP-EIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVET-VTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCF 1006
++ L + +PVM SW+PLQQNF VEDE L NIPYMG+ D D FIEEL++NY+G+VHG+ F N+ ++L+ L + K D L D K E N D +P + IF+AI+ F D +L +Y E L+E+ + TPN+D R+ Q E S+ H+F +L CRRCFKYDC +HP+ ++ + + N + PC +C+
Sbjct: 112 LKTLNAVASVPVMYSWSPLQQNFMVEDETVLHNIPYMGDEILDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALVQYSDNEDDEEDEEQDYKVDKMDLCDGKDHTEDPCKDGHSNESRANSDGSKKFPSDKIFEAISSMFPDKGSLEELKEKYKE---LTEQQLPGALPPECTPNIDGPN--------ARSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNLENLVESKPCGIDCY 352
BLAST of EZH vs. Ensembl Medaka
Match: ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:101166385]) HSP 1 Score: 240.35 bits (612), Expect = 4.146e-68 Identity = 110/146 (75.34%), Postives = 126/146 (86.30%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
+TCKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGE+ISQDEADRRG+IYDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I G+ELFFDYRY + LKYVGIERE++
Sbjct: 547 VTCKNCSIQRGLKKHLLLAPSDVAGWGTFIKEPVQKNEFISEYCGELISQDEADRRGRIYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAILQGEELFFDYRYSQADALKYVGIEREVD 692 HSP 2 Score: 120.939 bits (302), Expect = 7.408e-28 Identity = 67/191 (35.08%), Postives = 92/191 (48.17%), Query Frame = 2
Query: 1076 WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
W+ +E ++ VL + ++C++++L+ +K+C QVY + KE L I R + +++G+ ++SD SNQ Y PCDHP PCD C C NFCEKFCQC C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 401 WSGAEESLFRVLHGTYFNNFCSIARLIGTKTCKQVYEFAV---KEVL--------------------IHR-------------------VPFEDGDFSVSD------------NSSNQVYNYQPCDHPDHPCDNSCPCVMTQNFCEKFCQCENECQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCMTCG 537 HSP 3 Score: 108.997 bits (271), Expect = 4.259e-24 Identity = 98/337 (29.08%), Postives = 150/337 (44.51%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGE---DHDPKFIEELLRNYEGRVHGN----FPFDFNENQLIDLLKT---------------------LNEKYDWYKLADNKVKKHQEKIENFDY---PEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLE 1015
VK EY L+ LKK D+V+Q +++ + + + K++S+ V F K+ +R L + +P M SW+PLQ NF VEDE L NIPYMG+ + D F+EEL+ NY+G VHG+ F D +L+D L + EK D ++ + + E+ ++ + IF AIA F +L +Y + LL S TPNLD F ++ Q E S+ H+F +L CRRCFKYDC +HP+R++ +++ + PC + CF L+
Sbjct: 18 VKSEYMRLRQLKRLKKADEVKQ----QTTVLNSEWSTL-KIQSIPLSMCSVEFGFP-----------GFKAQAIAMRPLSTVAGIPFMYSWSPLQHNFMVEDETFLHNIPYMGDEVLEQDEAFLEELIDNYDG-VHGDREGGFISDEVFKELVDALSQYSDHEEDEEEEGAAAAATAVDVTEKKDDERMMRRSLVEGSEETKSGAKRIPSDKIFTAIASMFPYKGTTEELKEKYKD--LLEPSSQVKLPPLCTPNLDG--------PFAKSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFRATPNVYKRKSKEIRMETEPCGEECFLLQ 318
BLAST of EZH vs. Ensembl Medaka
Match: ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:101166385]) HSP 1 Score: 241.506 bits (615), Expect = 6.368e-68 Identity = 110/146 (75.34%), Postives = 126/146 (86.30%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
+TCKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGE+ISQDEADRRG+IYDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I G+ELFFDYRY + LKYVGIERE++
Sbjct: 622 VTCKNCSIQRGLKKHLLLAPSDVAGWGTFIKEPVQKNEFISEYCGELISQDEADRRGRIYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAILQGEELFFDYRYSQADALKYVGIEREVD 767 HSP 2 Score: 215.312 bits (547), Expect = 1.006e-58 Identity = 179/634 (28.23%), Postives = 264/634 (41.64%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS------KMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGED---HDPKFIEELLRNYEGRVHGN----FPFDFNENQLIDLLKT---------------------LNEKYDWYKLADNKVKKHQEKIENFDY---------------------PEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELT--------------------------------------NIEGDSLNENINHCDYNQ------------------------WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
VK EY L+ LKK D+V+ + N + I++Q +++ K++S+ V + +F K+ +R L + +P M SW+PLQ NF VEDE L NIPYMG++ D F+EEL+ NY+G VHG+ F D +L+D L + EK D ++ + + E+ ++ + + IF AIA F +L +Y + LL S TPNLD F ++ Q E S+ H+F +L CRRCFKYDC +HP+R++ +++ + PC + CF L+ + EGDS +E+ + + N W+ +E ++ VL + ++C++++L+ +K+C QVY + KE L H+ + GI +K K++ R KI +L KD SNQ Y PCDHP PCD C C NFCEKFCQC C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 38 VKSEYMRLRQLKRLKKADEVKTLFSSNREKIEQQTTVLNSEWSTLKIQSIPLSVSNVALANKKMCSVEFGFP-GFKAQAIAMRPLSTVAGIPFMYSWSPLQHNFMVEDETFLHNIPYMGDEVLEQDEAFLEELIDNYDG-VHGDREGGFISDEVFKELVDALSQYSDHEEDEEEEGAAAAATAVDVTEKKDDERMMRRSLVEGSEETKSGAFIRRKRRSMTEVRDLSGSKRIPSDKIFTAIASMFPYKGTTEELKEKYKD--LLEPSSQVKLPPLCTPNLDG--------PFAKSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFRATPNVYKRKSKEIRMETEPCGEECFLLQKGAKEFVDQNMRRSQRSRRRRRQQRATSSSCPGPSASAEESKEGDSDHESTSSSEGNSRSQTPTKQRPGDDCADQQACCVVPWSGAEESLFRVLHGTYFNNFCSIARLIGTKTCKQVYEFAV---KEVL----------IHRVPFEDGGILPQKKKRKHRLWAKI-QLKKD------------------------NSSNQVYNYQPCDHPDHPCDNSCPCVMTQNFCEKFCQCENECQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCMTCG 612
BLAST of EZH vs. Ensembl Medaka
Match: ezh1 (enhancer of zeste 1 polycomb repressive complex 2 subunit [Source:NCBI gene;Acc:101166385]) HSP 1 Score: 241.506 bits (615), Expect = 6.368e-68 Identity = 110/146 (75.34%), Postives = 126/146 (86.30%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIE 2323
+TCKN +IQRG +KHLL++ SDVAGWG + KE + KNEFI EYCGE+ISQDEADRRG+IYDK MSSFLFNLN FVVDATRKGNKIRFANHSVNPNC AK+++VNGDHRIGIFAK I G+ELFFDYRY + LKYVGIERE++
Sbjct: 622 VTCKNCSIQRGLKKHLLLAPSDVAGWGTFIKEPVQKNEFISEYCGELISQDEADRRGRIYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAILQGEELFFDYRYSQADALKYVGIEREVD 767 HSP 2 Score: 215.312 bits (547), Expect = 1.006e-58 Identity = 179/634 (28.23%), Postives = 264/634 (41.64%), Query Frame = 2
Query: 98 VKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVS------KMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGED---HDPKFIEELLRNYEGRVHGN----FPFDFNENQLIDLLKT---------------------LNEKYDWYKLADNKVKKHQEKIENFDY---------------------PEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELT--------------------------------------NIEGDSLNENINHCDYNQ------------------------WTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFY--HPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCA 1642
VK EY L+ LKK D+V+ + N + I++Q +++ K++S+ V + +F K+ +R L + +P M SW+PLQ NF VEDE L NIPYMG++ D F+EEL+ NY+G VHG+ F D +L+D L + EK D ++ + + E+ ++ + + IF AIA F +L +Y + LL S TPNLD F ++ Q E S+ H+F +L CRRCFKYDC +HP+R++ +++ + PC + CF L+ + EGDS +E+ + + N W+ +E ++ VL + ++C++++L+ +K+C QVY + KE L H+ + GI +K K++ R KI +L KD SNQ Y PCDHP PCD C C NFCEKFCQC C NRF GC+C+ CNTKQCPC+LA RECD DLC C
Sbjct: 38 VKSEYMRLRQLKRLKKADEVKTLFSSNREKIEQQTTVLNSEWSTLKIQSIPLSVSNVALANKKMCSVEFGFP-GFKAQAIAMRPLSTVAGIPFMYSWSPLQHNFMVEDETFLHNIPYMGDEVLEQDEAFLEELIDNYDG-VHGDREGGFISDEVFKELVDALSQYSDHEEDEEEEGAAAAATAVDVTEKKDDERMMRRSLVEGSEETKSGAFIRRKRRSMTEVRDLSGSKRIPSDKIFTAIASMFPYKGTTEELKEKYKD--LLEPSSQVKLPPLCTPNLDG--------PFAKSVQREQSL---------HSFHTLFCRRCFKYDCFLHPFRATPNVYKRKSKEIRMETEPCGEECFLLQKGAKEFVDQNMRRSQRSRRRRRQQRATSSSCPGPSASAEESKEGDSDHESTSSSEGNSRSQTPTKQRPGDDCADQQACCVVPWSGAEESLFRVLHGTYFNNFCSIARLIGTKTCKQVYEFAV---KEVL----------IHRVPFEDGGILPQKKKRKHRLWAKI-QLKKD------------------------NSSNQVYNYQPCDHPDHPCDNSCPCVMTQNFCEKFCQCENECQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCMTCG 612
BLAST of EZH vs. Planmine SMEST
Match: SMESG000045744.1 (SMESG000045744.1) HSP 1 Score: 1573.91 bits (4074), Expect = 0.000e+0 Identity = 775/779 (99.49%), Postives = 776/779 (99.61%), Query Frame = 2
Query: 41 MTKHRTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLXXXXXXXXX--XXXXXXXXITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN 2371
MTKHRTANKIEIC+NFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLSNSNNNNN TDSNTNNSITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN
Sbjct: 1 MTKHRTANKIEICDNFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLSNSNNNNNNNGTDSNTNNSITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN 779
BLAST of EZH vs. Planmine SMEST
Match: SMESG000045744.1 (SMESG000045744.1) HSP 1 Score: 1561.58 bits (4042), Expect = 0.000e+0 Identity = 771/779 (98.97%), Postives = 772/779 (99.10%), Query Frame = 2
Query: 41 MTKHRTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISKLAKDLDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLXXXXXXXXX--XXXXXXXXITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN 2371
MTKHRTANKIEIC+NFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISK LDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLSNSNNNNN TDSNTNNSITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN
Sbjct: 1 MTKHRTANKIEICDNFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIDEQIAMVSKMKSLSPFCEKVRFSQTYSEHDDFEVKINSKSYKSRVRLLPYIEKLPVMLSWAPLQQNFSVEDEIELTNIPYMGEDHDPKFIEELLRNYEGRVHGNFPFDFNENQLIDLLKTLNEKYDWYKLADNKVKKHQEKIENFDYPEIIFKAIAEAFGDVEDYGKLIARYLEMKLLSEKSASDGVETVTPNLDNEEEIREMEKFLRATQPENSIIEHKRDDATHTFRSLICRRCFKYDCAMHPYRSSKSMWNNANAYSSDVIGPCEKNCFKLELTNIEGDSLNENINHCDYNQWTLSERTMLNVLLPLFRTDYCALSQLLKSKSCAQVYSYVQAYKKEDLENMMTASDNQTHQTVNNNRGIKRKKMKQRKRPLPKISK----LDYKNGNDNLSDCEVIDHSAFGSKTDSNQFYHPCDHPGFPCDEKCNCRQVGNFCEKFCQCSRSCSNRFIGCKCRGHCNTKQCPCFLANRECDQDLCQCCAPKHINRSYSLKSLTNLDMNGITTKFSSLSNSGAENSHDSFISHSVSTNGLYKTQVSNDSFQKDLSNSNNNNNNNGTDSNTNNSITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRYGPTEQLKYVGIEREIETQNLSGNQSSNRKYSN 775
BLAST of EZH vs. Planmine SMEST
Match: SMESG000074842.1 (SMESG000074842.1) HSP 1 Score: 108.997 bits (271), Expect = 1.050e-24 Identity = 56/136 (41.18%), Postives = 80/136 (58.82%), Query Frame = 2
Query: 1886 ITCKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSSFLFNL----NQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNG----DHRIGIFAKHNIKAGDELFFDY 2269
+ C +QRG + L + + GWG+ T I++ FI EY GEI++ DEA+ RGK+YD+ ++LF+L + F VDA +KGN F NHS NPN ++ + +N RI FA +IKAG+EL FDY
Sbjct: 289 LNCPYRVVQRGVKLQLKIFRTKNRGWGLKTLTKIIRGNFIMEYLGEIVAVDEAEERGKLYDQQNLTYLFDLDFAGDPEFTVDAYKKGNMSHFINHSCNPNMTSRCVFINNLDIRMPRIAFFAARDIKAGEELTFDY 424
BLAST of EZH vs. Planmine SMEST
Match: SMESG000039925.1 (SMESG000039925.1) HSP 1 Score: 97.4413 bits (241), Expect = 2.290e-20 Identity = 52/130 (40.00%), Postives = 75/130 (57.69%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRR-GKIYDKTMSSF-LFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRY 2275
C+N R + + +D GW + T E I K F+ EY GE+I + EA+RR K ++ +S+F + L+ ++DA N RF NHS +PN + VNGD RIG+FA H IKAG+EL F+Y +
Sbjct: 786 CRNQRFMRREYPKQEVFSTDNRGWALRTLEPIKKGAFVNEYVGELIDEAEANRRLKKAHETNVSNFYMMELDGQRIIDAGPAANISRFMNHSCSPNVYTQKWSVNGDTRIGLFALHTIKAGEELTFNYNF 915
BLAST of EZH vs. Planmine SMEST
Match: SMESG000039925.1 (SMESG000039925.1) HSP 1 Score: 97.0561 bits (240), Expect = 2.646e-20 Identity = 52/130 (40.00%), Postives = 73/130 (56.15%), Query Frame = 2
Query: 1892 CKNMAIQRGQRKHLLMSVSDVAGWGIYTKEDIVKNEFIYEYCGEIISQDEADRRGKIYDKTMSS--FLFNLNQSFVVDATRKGNKIRFANHSVNPNCCAKILLVNGDHRIGIFAKHNIKAGDELFFDYRY 2275
C+N R + + +D GW + T E I K F+ EY GE+I + EA+RR K +T S ++ L+ ++DA N RF NHS +PN + VNGD RIG+FA H IKAG+EL F+Y +
Sbjct: 801 CRNQRFMRREYPKQEVFSTDNRGWALRTLEPIKKGAFVNEYVGELIDEAEANRRLKKAHETNVSNFYMMELDGQRIIDAGPAANISRFMNHSCSPNVYTQKWSVNGDTRIGLFALHTIKAGEELTFNYNF 930 The following BLAST results are available for this feature:
BLAST of EZH vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of EZH vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of EZH vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of EZH vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of EZH vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of EZH vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of EZH vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of EZH vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of EZH vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of EZH vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of EZH vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of EZH vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of EZH vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of EZH vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30004132 ID=SMED30004132|Name=EZH|organism=Schmidtea mediterranea sexual|type=transcript|length=2544bpback to top protein sequence of SMED30004132-orf-1 >SMED30004132-orf-1 ID=SMED30004132-orf-1|Name=SMED30004132-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=778bp MTKHRTANKIEICENFVEIVKLEYNSLQDQIILKKKDDVRQILKHNLDIIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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