Tubulin beta chain

Overview
NameTubulin beta chain
Smed IDSMED30004118
Length (bp)1621
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tubulin beta chain (SMED30004118) t-SNE clustered cells

Violin plots show distribution of expression levels for Tubulin beta chain (SMED30004118) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tubulin beta chain (SMED30004118) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tubulin beta chain (SMED30004118) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30004118

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 20

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
headSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
pharynxSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parapharyngeal cellSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
pharynxSMED30004118SMESG000022928.1 SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v6_83_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004118SMESG000022928.1 SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v6_83_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30004118SMESG000022928.1 SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v6_83_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30004118SMESG000022928.1 SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v6_83_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004118SMESG000022928.1 SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v6_83_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neural progenitor cellSMED30004118SMESG000022928.1 SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v6_83_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30004118SMESG000022928.1 SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v6_83_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30004118SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044888.1 SMESG000035755.1 dd_Smed_v4_83_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB4B (tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:HGNC:20771])

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 427/427 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB2B (tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:HGNC:30829])

HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0
Identity = 415/430 (96.51%), Postives = 429/430 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVY+NEATG KYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 413/429 (96.27%), Postives = 428/429 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VY+NEATGGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 413/429 (96.27%), Postives = 428/429 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VY+NEATGGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 413/429 (96.27%), Postives = 428/429 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VY+NEATGGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-4 (Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot;Acc:P41937])

HSP 1 Score: 832.787 bits (2150), Expect = 0.000e+0
Identity = 408/430 (94.88%), Postives = 426/430 (99.07%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG Y+GDSDLQLERINVY+NEA+GGKYVPRA L+DLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAESCDCLQGFQ+THSLGGGTGSGMGTLLISKIREEYPDRIM TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR+LTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRG+KM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+ATA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGAYNGDSDLQLERINVYYNEASGGKYVPRACLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIMMTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSMTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGVKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: ben-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q18817])

HSP 1 Score: 817.379 bits (2110), Expect = 0.000e+0
Identity = 400/430 (93.02%), Postives = 421/430 (97.91%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGI P GTY G+SDLQLERINVY+NEA GGKYVPRAVL+DLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM++FSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKL+ PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVD+QM+NVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+ATA
Sbjct:    1 MREIVHVQAGQCGNQIGAKFWEVISDEHGIQPDGTYKGESDLQLERINVYYNEANGGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLSNPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDDQMMNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: mec-7 (Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot;Acc:P12456])

HSP 1 Score: 815.839 bits (2106), Expect = 0.000e+0
Identity = 403/426 (94.60%), Postives = 416/426 (97.65%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 1473
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDP+G Y GDSDLQLERINVY+NEA   KYVPRAVL+DLEPGTMDSVRSGPFGQLFRPDN+VFGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAES DCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTD TFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSR +QQYRA+TVPELTQQ FDAKNMMAACDPRHGRYLT AAIFRGRMSMKEVDEQMLN+QNKNSSYFV+WIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ
Sbjct:    1 MREIVHIQAGQCGNQIGSKFWEVISDEHGIDPSGQYVGDSDLQLERINVYYNEAGSNKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNYVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESTDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDSTFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRSNQQYRAITVPELTQQCFDAKNMMAACDPRHGRYLTAAAIFRGRMSMKEVDEQMLNIQNKNSSYFVDWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 810.446 bits (2092), Expect = 0.000e+0
Identity = 394/430 (91.63%), Postives = 420/430 (97.67%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIGSKFWEVISDEHGI P GT+ G++DLQLERI+VY+NEA  GKYVPRAVL+DLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLD++RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM++FSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDIC+RTLKLT PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDL+SEYQQYQ+ATA
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 810.446 bits (2092), Expect = 0.000e+0
Identity = 394/430 (91.63%), Postives = 420/430 (97.67%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIGSKFWEVISDEHGI P GT+ G++DLQLERI+VY+NEA  GKYVPRAVL+DLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLD++RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIM++FSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDIC+RTLKLT PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDL+SEYQQYQ+ATA
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086536)

HSP 1 Score: 841.262 bits (2172), Expect = 0.000e+0
Identity = 412/429 (96.04%), Postives = 426/429 (99.30%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIVH+QAGQCGNQIG+KFWE+ISDEHGID TG YHGDSDLQLERINVY+NEA+GGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNT+SVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLN+QNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 429          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub85D (gene:FBgn0003889 transcript:FBtr0082046)

HSP 1 Score: 831.632 bits (2147), Expect = 0.000e+0
Identity = 411/430 (95.58%), Postives = 423/430 (98.37%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG KFWEVISDEH ID TGTY+GDSDLQLERINVY+NEATG KYVPRA+L+DLEPGTMDSVRSG FGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+E CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLN+QNKNSS+FVEWIPNN KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+ATA
Sbjct:    1 MREIVHIQAGQCGNQIGGKFWEVISDEHCIDATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSFFVEWIPNNCKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0072270)

HSP 1 Score: 800.816 bits (2067), Expect = 0.000e+0
Identity = 391/436 (89.68%), Postives = 422/436 (96.79%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEAT------GGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIV++QAGQCGNQIG+KFWE+IS+EHGID  G Y GDSDLQLER++VY+NEA+      GGKYVPRA+L+DLEPGTM+SVRSGP+GQLFRPDNFV+GQSGAGNNWAKGHYTEGAELVD+VLD+VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNT+SVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLK++ P+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML VQNKNSSYFVEWIPNN+KTAVCDIPP+GLKMS+TFIGN+TAIQELFKR+SEQF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+ATA
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASAVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 436          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0343443)

HSP 1 Score: 800.816 bits (2067), Expect = 0.000e+0
Identity = 391/435 (89.89%), Postives = 422/435 (97.01%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEAT-----GGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIV++QAGQCGNQIG+KFWE+IS+EHGID  G Y GDSDLQLER++VY+NEA+     GGKYVPRA+L+DLEPGTM+SVRSGP+GQLFRPDNFV+GQSGAGNNWAKGHYTEGAELVD+VLD+VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNT+SVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLK++ P+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML VQNKNSSYFVEWIPNN+KTAVCDIPP+GLKMS+TFIGN+TAIQELFKR+SEQF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+ATA
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEATA 435          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086537)

HSP 1 Score: 800.431 bits (2066), Expect = 0.000e+0
Identity = 395/410 (96.34%), Postives = 407/410 (99.27%), Query Frame = 1
Query:  253 FWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            FWE+ISDEHGID TG YHGDSDLQLERINVY+NEA+GGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNT+SVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLN+QNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+AT
Sbjct:   29 FWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAT 438          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 852.432 bits (2201), Expect = 0.000e+0
Identity = 419/430 (97.44%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 852.432 bits (2201), Expect = 0.000e+0
Identity = 419/430 (97.44%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 852.432 bits (2201), Expect = 0.000e+0
Identity = 419/430 (97.44%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 852.432 bits (2201), Expect = 0.000e+0
Identity = 419/430 (97.44%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: tubb4b (tubulin, beta 4B class IVb [Source:ZFIN;Acc:ZDB-GENE-030131-8625])

HSP 1 Score: 850.121 bits (2195), Expect = 0.000e+0
Identity = 417/430 (96.98%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:Xenbase;Acc:XB-GENE-985000])

HSP 1 Score: 853.973 bits (2205), Expect = 0.000e+0
Identity = 420/430 (97.67%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: znf330 (zinc finger protein 330 [Source:Xenbase;Acc:XB-GENE-1010548])

HSP 1 Score: 846.269 bits (2185), Expect = 0.000e+0
Identity = 415/430 (96.51%), Postives = 429/430 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVY+NEATG K+VPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb (tubulin beta class I [Source:NCBI gene;Acc:448742])

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 413/429 (96.27%), Postives = 428/429 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VY+NEATGGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: ubxn11 (UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-985480])

HSP 1 Score: 841.262 bits (2172), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 427/427 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEA+GGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSV+D+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR+LTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVMDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDSKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb3 (tubulin beta 3 class III [Source:NCBI gene;Acc:395045])

HSP 1 Score: 812.372 bits (2097), Expect = 0.000e+0
Identity = 397/430 (92.33%), Postives = 417/430 (96.98%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDP G Y GDSDLQLERI+VY+NEA+  KYVPRA+L+DLEPGTMDSVRSG FG LFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISK+REEYPDRIMNTFSVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLKL TPTYGDLNHLVSATMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT+RGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVA +FRGRMSMKEVDEQML +Q+KNSSYFVEWIPNN+K AVCDIPPRGLKMS+TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPCGNYIGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4b (tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:MGI:1915472])

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 427/427 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2b (tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:MGI:1920960])

HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0
Identity = 415/430 (96.51%), Postives = 429/430 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVY+NEATG KYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb5 (tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI:107812])

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 413/429 (96.27%), Postives = 428/429 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VY+NEATGGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2a (tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:MGI:107861])

HSP 1 Score: 842.032 bits (2174), Expect = 0.000e+0
Identity = 413/430 (96.05%), Postives = 427/430 (99.30%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVY+NEA G KYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+ IDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4a (tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:MGI:107848])

HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGG YVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE+PDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML+VQ+KNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P11833|TBB_PARLI (Tubulin beta chain OS=Paracentrotus lividus OX=7656 PE=2 SV=1)

HSP 1 Score: 856.284 bits (2211), Expect = 0.000e+0
Identity = 422/430 (98.14%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVHMQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHMQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P30883|TBB4_XENLA (Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=tubb4 PE=2 SV=1)

HSP 1 Score: 852.432 bits (2201), Expect = 0.000e+0
Identity = 420/430 (97.67%), Postives = 429/430 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG YHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68372|TBB4B_MOUSE (Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=Tubb4b PE=1 SV=1)

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 427/427 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68371|TBB4B_HUMAN (Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=TUBB4B PE=1 SV=1)

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 427/427 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q3MHM5|TBB4B_BOVIN (Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUBB4B PE=2 SV=1)

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 427/427 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A2U9BBA2 (Ras GTPase-activating-like protein IQGAP1 OS=Scophthalmus maximus OX=52904 GN=SMAX5B_007995 PE=3 SV=1)

HSP 1 Score: 862.833 bits (2228), Expect = 0.000e+0
Identity = 414/430 (96.28%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RINVY+NEA+GGKYVPRAVL+DLEPGTMD+VRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTP+YGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR+LTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDAVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPSYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A267E855 (Tubulin beta chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021796g2 PE=3 SV=1)

HSP 1 Score: 858.21 bits (2216), Expect = 0.000e+0
Identity = 421/432 (97.45%), Postives = 432/432 (100.00%), Query Frame = 1
Query:  190 LKMREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            ++MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:   32 VRMREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 463          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A1I8FZR8 (Tubulin beta chain OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 858.21 bits (2216), Expect = 0.000e+0
Identity = 421/430 (97.91%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:   61 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 490          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A369S2L5 (Tubulin beta chain OS=Trichoplax sp. H2 OX=287889 GN=TrispH2_006238 PE=3 SV=1)

HSP 1 Score: 856.284 bits (2211), Expect = 0.000e+0
Identity = 419/432 (96.99%), Postives = 432/432 (100.00%), Query Frame = 1
Query:  190 LKMREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            +KMREIVH+QAGQCGNQIG+KFWEVISDEHG+DPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TF+GNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:   21 VKMREIVHLQAGQCGNQIGAKFWEVISDEHGVDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFVGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 452          
BLAST of Tubulin beta chain vs. TrEMBL
Match: W4X9L8 (Tubulin beta chain OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1)

HSP 1 Score: 856.284 bits (2211), Expect = 0.000e+0
Identity = 422/430 (98.14%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVHMQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHMQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: zgc:55461 (tubulin beta-4B chain [Source:NCBI gene;Acc:103024068])

HSP 1 Score: 850.892 bits (2197), Expect = 0.000e+0
Identity = 418/430 (97.21%), Postives = 429/430 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG YHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB2A (tubulin beta-2A chain [Source:NCBI gene;Acc:103041736])

HSP 1 Score: 833.173 bits (2151), Expect = 0.000e+0
Identity = 407/430 (94.65%), Postives = 426/430 (99.07%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGID TG+Y G+S+LQLERINVY+NEATG K+VPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSV+PSPKVSDTVVEPYNATLSVHQLVENTDETF IDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRAL+VPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQML+VQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELF+R+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:   78 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGSYQGESELQLERINVYYNEATGSKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 507          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB1 (tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HGNC:16257])

HSP 1 Score: 693.345 bits (1788), Expect = 0.000e+0
Identity = 343/430 (79.77%), Postives = 373/430 (86.74%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+Q GQCGNQIGSK  E+                             +  GGKYVPRA+L+DLEPGTMDSVR    GQLFRPDNF+ G SGAGNNWAKGHYTEGAELV+ V+D VR EAESCDCLQGFQ  HSLGGGTGSGMGTL+I+KIREEYPDRIMN+FS++PSPKVSDTVVEPYNATLS+HQL+ENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT+RGSQQYRALTVPELTQQMFDA+NMM ACDPR GRYLTVA IFRGRMS KEVDEQML +Q KNS+YFV+WIP+N+K AVCDIPPRGLKM++TFIGN+TAIQE+F+RV EQF  MFRRKAFLHWYTGEGMDEMEFTEAE+N+NDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQIGQCGNQIGSKVTEI--------------------------RLIDTCGGKYVPRALLVDLEPGTMDSVRGSRIGQLFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRNEAESCDCLQGFQFVHSLGGGTGSGMGTLIINKIREEYPDRIMNSFSIMPSPKVSDTVVEPYNATLSIHQLIENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGRMSTKEVDEQMLAIQQKNSNYFVDWIPHNVKVAVCDIPPRGLKMASTFIGNNTAIQEIFRRVGEQFAMMFRRKAFLHWYTGEGMDEMEFTEAENNLNDLVSEYQQYQDATA 404          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000019550.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000026.1:1405913:1407004:-1 gene:ENSAMXG00000018987.2 transcript:ENSAMXT00000019550.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 652.514 bits (1682), Expect = 0.000e+0
Identity = 326/335 (97.31%), Postives = 334/335 (99.70%), Query Frame = 1
Query:  472 GQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            GQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:   12 GQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 346          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000030346.1 (tubulin alpha-1B chain [Source:NCBI gene;Acc:111195381])

HSP 1 Score: 356.681 bits (914), Expect = 4.701e-118
Identity = 180/434 (41.47%), Postives = 266/434 (61.29%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1467
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +F+E   GK+VPRAV +DLEP  +D VR+G + QLF P+  + G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    FS+ P+P+VS  VVEPYN+ L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    ++RG +  K+V+  +  ++ K +  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0
Identity = 352/375 (93.87%), Postives = 367/375 (97.87%), Query Frame = 1
Query:  250 KFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVV-PSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRK 1371
            +FWEVISDEHGIDPTG+YHGDSDLQLERINVY+NEA+GGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEY  +++ T     PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRK
Sbjct:    1 QFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYIIQVLYTVVASGPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRK 375          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 298.13 bits (762), Expect = 2.576e-97
Identity = 151/348 (43.39%), Postives = 218/348 (62.64%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIP 1233
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +F+E   GK+VPRAV +DLEP  +D VR+G + QLF P+  + G+  A NN+A+GHYT G E+VD VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    F++ P+P+VS  VVEPYN+ L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+   +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    ++RG +  K+V+  +  ++ K +  FV+W P
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCP 348          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 261.922 bits (668), Expect = 4.590e-82
Identity = 146/439 (33.26%), Postives = 239/439 (54.44%), Query Frame = 1
Query:  199 REIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQ--SGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSP-KVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGS-QQYRALTVPELTQQMFDAKNMMAACDPRHGR-------YLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPP---RGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGM---DEMEFTEAESNMNDLVSEYQ 1464
            REI+ +Q GQCGNQIG +FW+ +  EHGI P G     +    +R +V+F +A    Y+PRAVL+DLEP  ++S+ +  +  L+ P+N    +   GAGNNWA GH ++G ++ + + DI+ +EA+  D L+             S      + K    +P +++ T+SV P+  ++SD VV+PYN+ L++ +L +N D    +DN AL  I    L +  P++  +N LVS  MS  TT LR+PG +N DL  L  +++P PRLHF M G+ PLT+  S    R  TV ++ +++   KN+M +     GR       Y+ +  I +G +   +V + +  ++ +  + F+ W P +I+ A+    P      ++S   + N T+I  LF R  +Q+  + +R+AFL  +  E M   D  EF ++   +  L+ EYQ
Sbjct:    3 REIITLQLGQCGNQIGFEFWKQLCAEHGISPDGILEDFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVINSIVNSSYANLYNPENIYLSEHGGGAGNNWASGH-SQGEKIHEEIFDIIDREADGSDSLE-----KCPLTRRLSATNWNELCKYFCRFPKKLIQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVST----GRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLTSAHRVSGLMMANHTSISSLFDRTCKQYDKLRKREAFLDQFRKEDMFKEDLSEFDDSREVVQQLIDEYQ 431          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009623.1 (pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 gene:ENSPMAG00000008696.1 transcript:ENSPMAT00000009623.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 251.136 bits (640), Expect = 7.825e-82
Identity = 135/159 (84.91%), Postives = 152/159 (95.60%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHG-DSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 669
            MREIVH+QAGQCGNQ+GSKFWEVIS+EHGID TG+Y G ++ LQ+ERINVY++EAT GK+VPRA+++DLEPGTMD+VRSGP G +FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAE+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREE
Sbjct:    1 MREIVHVQAGQCGNQVGSKFWEVISEEHGIDRTGSYRGYNARLQMERINVYYSEATSGKFVPRAIMVDLEPGTMDAVRSGPLGHMFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE 159          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 233.032 bits (593), Expect = 4.891e-72
Identity = 129/351 (36.75%), Postives = 201/351 (57.26%), Query Frame = 1
Query:  199 REIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL---ERINVYFNEATGGKYVPRAVLIDLEPGTMD---SVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIP 1233
            R+ + +  GQ G Q+G+  WE+   EHGI P G      D+        N +F+E   GK+VPRA+ IDLEP  +     +R+   G   R    + G+    +   +G    G ++   VL   R ++E C  LQGF L HS GGGTGSG  +LL+ ++  +Y  +    F + P+P+VS  VVEPYNA L+ H  +E++D +F +DNEA+YDIC + L +  P+Y +LN L++  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPR+G+Y+    ++RG +  K+V+  +  ++++ +  FV+W P
Sbjct:    3 RDCISIHIGQAGVQMGNACWELYCLEHGIKPDGHLATHPDVTSVVDSSFNTFFSETGSGKHVPRAIFIDLEPSVIGEPCGLRTLTVGGXLRMSMMLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSGFTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVISAEKAYHEQLSVAEITNACFEPSNQMVKCDPRNGKYMACCLLYRGDIVPKDVNTAIAIIKSRRAVRFVDWCP 353          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 688.723 bits (1776), Expect = 0.000e+0
Identity = 331/429 (77.16%), Postives = 387/429 (90.21%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREI+H+  GQCGNQIG+ FWE I  EHG+D  GTYHG  D+Q ER+NVYFNEA+ GK+VPR++ +DLEPGT+D+VR+   G LFRPDN++FGQS AGN WAKGHYTEGAELVDSV+D++R+EAE CD LQGFQ+THSLGGGTGSGMGTLLISKIREE+PDR+M TFSV+PSPK SDTVVEPYNATLSVHQLVE++DETFCIDNEALYDIC RTLKL  P+YGDLN+LVS+ MSGVTT LR+PGQLN+DLRKLAVN+VPFPRLHFFM G+APLT+ GSQ +R+LTVPELTQQMFDAKNMMAA DPR+GRYLTVAA FRG++S+KEV+++M  VQ+KNS YFVEWIPNN++TAVC + P+GL M+ATFI NST+IQELFKRV +QF+AMF+RKAFLHWYT EGMDE+EF+EAESNMNDLVSEYQQYQ+AT
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSEAESNMNDLVSEYQQYQEAT 429          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 350.903 bits (899), Expect = 8.696e-117
Identity = 181/435 (41.61%), Postives = 271/435 (62.30%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL-----ERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRG--------LKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEY 1461
            MRE++ +  GQ G QIG+  WE+ S EHGI P G  H +  L       E  + +F+E   GK+VPRA+ +DLEP  +D VR+GP+  LF P+  + G+  A NN+A+GHYT G E++  VLD +RK A+ CD LQGF  THSLGGGTGSG+G+LL+ ++  EY  +    F+V P+P+VS +VVEPYN  L+ H  +E+ D TF +DNEA+YD+C R L +  P++ +LN+L++  +S VT  LRF G LN DL +   N+VP+PR+HF +  ++P+ S+    + + +V E+T   F+  N M  CDPR G+Y+    ++RG +  ++V   +  V+NK +   V+W P   K  +C  PP          +  +   + N+T+I E +KR+  +F  M+ ++AF+HWY GEGM+E EFTEA  ++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSKAFHESNSVSEITNACFEPGNQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEPPTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 345.51 bits (885), Expect = 1.362e-114
Identity = 181/435 (41.61%), Postives = 269/435 (61.84%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL-----ERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMS--------ATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEY 1461
            MRE++ +  GQ G QIG+  WE+ S EHGI   G  H +  L       E  + +F+E   GK+VPRA+ +DLEP  +D VR+G F +LF P+  + G+  A NN+A+GHYT G E+VD V + +RK A+ CD LQGF  THSLGGGTGSG+G+LL+  +  EY  +    F+V P+P++S +VVEPYN  L+ H  +E+ D TF +DNEA+YDIC R L ++ P++ +LN L++  +S VT  LRF G LN DL +   N+VP+PR+HF +  +AP+ S+    + + +V E+T   F+  N M  CDP  G+Y+    ++RG +  ++V   +  V+NK +   V+W P   K  +C  PP  +  S           + N+TAI + +KR+ ++F  M+ ++AF+HWY GEGM+E EFTEA  ++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 171.4 bits (433), Expect = 6.498e-48
Identity = 129/440 (29.32%), Postives = 214/440 (48.64%), Query Frame = 1
Query:  202 EIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLER---INVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGA--GNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTS---RGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPN----NI--KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWY-TGEGMDEM-----EFTEAESNMNDLVSEY 1461
            EI+ +QAGQCGN +G   W  ++ EH I   G      D   ER      +F E +  K+ PRA+++D EP  +  V +  F   F P N      GA  GN+WA G Y  G    D +L+ + KE +S D  +GFQL HS+ GGTGSG+G+ L+  + + YP +I+ T+SV P+ + S+ VV+ YN  L++ +L+E++D T   DN +L +I  +  +         N L+S  +S VT  +RFP  + + +  +   ++P P LHF  P F P TS        ++  +  ++   + D  N + +    +  Y  V     G +  +++   M  +Q +    F  W  +    NI  ++    + P   ++S   + N + +  +F+     F  +F + AFL+ Y  G+    M     EF E+   +  L+ +Y
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQL-PDSSTERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVEN-TFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSMQNVQDEFAESREVVQSLMEDY 437          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO39945 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8I3])

HSP 1 Score: 850.892 bits (2197), Expect = 0.000e+0
Identity = 419/430 (97.44%), Postives = 429/430 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHCQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO35958 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJX9])

HSP 1 Score: 844.729 bits (2181), Expect = 0.000e+0
Identity = 415/429 (96.74%), Postives = 428/429 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIV +QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPR+VLIDLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAEL+DSVLD+VRKEAESCDC+QGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFS+VPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MREIVSLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRSVLIDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELMDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSIVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 429          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO42890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S027])

HSP 1 Score: 827.78 bits (2137), Expect = 0.000e+0
Identity = 408/429 (95.10%), Postives = 421/429 (98.14%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            MREIVH+QAGQCGNQIG+KFW    +EHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDC+QGFQLTHSLGGGTGSGM TLLI KIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRL FFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDP HGRYLTVAA+FRGRMSMKE+DEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MREIVHLQAGQCGNQIGAKFW----NEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMVTLLILKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLPFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPLHGRYLTVAAMFRGRMSMKELDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 425          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO49518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGG3])

HSP 1 Score: 687.182 bits (1772), Expect = 0.000e+0
Identity = 328/432 (75.93%), Postives = 380/432 (87.96%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTG--TYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREI+++Q GQCGNQIG++FW  I++EHGIDPTG  +   D+  + +RI+VYF+E  GG+YVPR++L+DLEPGTMD+VR GP G +FRPDNFV  QSGA NNWAKGHY+EGAEL+D++ D++RKEAE+CDC+QG QL  +LGGGTGSG+GTL++SKIREEYPDRI++ FSVVPSPKVS+TVVEPYNATLSVHQLVE  DE F  DNEALYDIC RTLKL  P Y DLN LVS  M G+T  LRFPGQLN DLRKLAVNMVPFPRLHFFMPGFAPL SR +QQY+ ++V ELTQQMFDA+NMM ACDPRHGRYLTVAAIFRG+MSM+EVDEQMLN QNKNS +FVEWIPNN+KT VCDI P+GLKM+ATF+GN+TAIQELFKRV EQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNM+DL++EYQQYQ+A A
Sbjct:    1 MREIINIQVGQCGNQIGTEFWRDIAEEHGIDPTGAKSLIEDTHGRFDRIDVYFHEGNGGRYVPRSILVDLEPGTMDAVRGGPLGNMFRPDNFVNSQSGAANNWAKGHYSEGAELIDNIFDVLRKEAENCDCMQGVQLIQALGGGTGSGLGTLILSKIREEYPDRILSAFSVVPSPKVSETVVEPYNATLSVHQLVEVVDEDFMFDNEALYDICCRTLKLAKPNYADLNQLVSMAMCGLTASLRFPGQLNGDLRKLAVNMVPFPRLHFFMPGFAPLASRANQQYQNVSVEELTQQMFDARNMMTACDPRHGRYLTVAAIFRGKMSMREVDEQMLNTQNKNSPFFVEWIPNNVKTTVCDIAPKGLKMAATFVGNNTAIQELFKRVGEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMHDLIAEYQQYQEAEA 432          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO36734 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHY4])

HSP 1 Score: 662.144 bits (1707), Expect = 0.000e+0
Identity = 332/339 (97.94%), Postives = 338/339 (99.71%), Query Frame = 1
Query:  466 VFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 1482
            + GQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT
Sbjct:    1 MLGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 339          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb2 (tubulin beta-2B chain [Source:NCBI gene;Acc:101162245])

HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0
Identity = 411/430 (95.58%), Postives = 428/430 (99.53%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+Y GDSDLQLERINVY+NEA+G KYVPRA+L+DLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVD+VLD+VRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSV+PSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRAL+VPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELF+R+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYQGDSDLQLERINVYYNEASGSKYVPRAILVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb5 (tubulin beta chain [Source:NCBI gene;Acc:101157969])

HSP 1 Score: 838.565 bits (2165), Expect = 0.000e+0
Identity = 410/427 (96.02%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1476
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VY+NEATGGKYVPRA+L+DLEPGTMDSVRSGPFGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000045809.1 (tubulin beta-6 chain [Source:NCBI gene;Acc:101166121])

HSP 1 Score: 749.584 bits (1934), Expect = 0.000e+0
Identity = 362/430 (84.19%), Postives = 398/430 (92.56%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+Q GQCGNQIGSKFWEVIS+EHGI+ TG Y GDS LQLERINVYF+EA GGKYVPRA+L+DLEPGTMDS+R    G LFRPDNF+ G SGAGNNWAKGHYTEGAELV+ V+D VR E+E CDCLQGFQL HSLGGGTGSGMGTL+I+KI+EE+PDRIMNTFS++PSPKVSDTVVEPYNATLSVHQL+EN D TFCIDNEALYDICFRTLKLTTPTYGDLNHLV  TMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT+RGSQQYRALTVPELTQQMFDA+NMM ACDPR GRYLTVA IFRG+MS KEVDEQML +Q KNS+YFV+WIP+N+K AVCDIPP+GL+M++TFIGN+TAIQE+F+RV EQF+ MFRRKAFLHWYTGEGMDEMEFTEAESN+NDLVSEYQQYQ+ATA
Sbjct:    1 MREIVHVQIGQCGNQIGSKFWEVISEEHGINATGIYEGDSTLQLERINVYFSEAYGGKYVPRALLVDLEPGTMDSIRGSRIGALFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRHESEGCDCLQGFQLVHSLGGGTGSGMGTLIINKIKEEFPDRIMNTFSIMPSPKVSDTVVEPYNATLSVHQLLENADATFCIDNEALYDICFRTLKLTTPTYGDLNHLVCMTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGKMSTKEVDEQMLAIQQKNSTYFVDWIPHNVKVAVCDIPPKGLRMASTFIGNNTAIQEIFRRVGEQFSLMFRRKAFLHWYTGEGMDEMEFTEAESNLNDLVSEYQQYQEATA 430          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000033163.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 358.992 bits (920), Expect = 5.806e-119
Identity = 182/434 (41.94%), Postives = 266/434 (61.29%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1467
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +F+E   GK+VPRAV +DLEP  +D VR+G + QLF P+  + G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    FS+ P+P+VS  VVEPYNA L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    ++RG +  K+V+  +  ++ K S  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000020578.2 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 358.992 bits (920), Expect = 5.806e-119
Identity = 182/434 (41.94%), Postives = 266/434 (61.29%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1467
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +F+E   GK+VPRAV +DLEP  +D VR+G + QLF P+  + G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +    FS+ P+P+VS  VVEPYNA L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    ++RG +  K+V+  +  ++ K S  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047284.1 (SMESG000047284.1)

HSP 1 Score: 858.596 bits (2217), Expect = 0.000e+0
Identity = 426/430 (99.07%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000046374.1 (SMESG000046374.1)

HSP 1 Score: 858.596 bits (2217), Expect = 0.000e+0
Identity = 426/430 (99.07%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVL+DLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047291.1 (SMESG000047291.1)

HSP 1 Score: 857.44 bits (2214), Expect = 0.000e+0
Identity = 426/430 (99.07%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000044888.1 (SMESG000044888.1)

HSP 1 Score: 857.44 bits (2214), Expect = 0.000e+0
Identity = 426/430 (99.07%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFF+PGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFIPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000035755.1 (SMESG000035755.1)

HSP 1 Score: 856.284 bits (2211), Expect = 0.000e+0
Identity = 424/430 (98.60%), Postives = 430/430 (100.00%), Query Frame = 1
Query:  196 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYFNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 1485
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVY+NEATGGKYVPRAVLIDLEPGTMDSVR+GPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATA 430          
The following BLAST results are available for this feature:
BLAST of Tubulin beta chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBB4B0.000e+097.66tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:... [more]
TUBB2B0.000e+096.51tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:... [more]
TUBB0.000e+096.27tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+096.27tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+096.27tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Tubulin beta chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tbb-40.000e+094.88Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot... [more]
ben-10.000e+093.02Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q... [more]
mec-70.000e+094.60Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot... [more]
tbb-20.000e+091.63Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
tbb-20.000e+091.63Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
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BLAST of Tubulin beta chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
betaTub56D0.000e+096.04gene:FBgn0284243 transcript:FBtr0086536[more]
betaTub85D0.000e+095.58gene:FBgn0003889 transcript:FBtr0082046[more]
betaTub60D0.000e+089.68gene:FBgn0003888 transcript:FBtr0072270[more]
betaTub60D0.000e+089.89gene:FBgn0003888 transcript:FBtr0343443[more]
betaTub56D0.000e+096.34gene:FBgn0284243 transcript:FBtr0086537[more]
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BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554610.000e+097.44zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+097.44zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+097.44zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+097.44zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
tubb4b0.000e+096.98tubulin, beta 4B class IVb [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Tubulin beta chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
reps20.000e+097.67RALBP1 associated Eps domain containing 2 [Source:... [more]
znf3300.000e+096.51zinc finger protein 330 [Source:Xenbase;Acc:XB-GEN... [more]
tubb0.000e+096.27tubulin beta class I [Source:NCBI gene;Acc:448742][more]
ubxn110.000e+096.25UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-... [more]
tubb30.000e+092.33tubulin beta 3 class III [Source:NCBI gene;Acc:395... [more]
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BLAST of Tubulin beta chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tubb4b0.000e+097.66tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:... [more]
Tubb2b0.000e+096.51tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:... [more]
Tubb50.000e+096.27tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI... [more]
Tubb2a0.000e+096.05tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:... [more]
Tubb4a0.000e+096.25tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:... [more]
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BLAST of Tubulin beta chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P11833|TBB_PARLI0.000e+098.14Tubulin beta chain OS=Paracentrotus lividus OX=765... [more]
sp|P30883|TBB4_XENLA0.000e+097.67Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=... [more]
sp|P68372|TBB4B_MOUSE0.000e+097.66Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=... [more]
sp|P68371|TBB4B_HUMAN0.000e+097.66Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=T... [more]
sp|Q3MHM5|TBB4B_BOVIN0.000e+097.66Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUB... [more]
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BLAST of Tubulin beta chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2U9BBA20.000e+096.28Ras GTPase-activating-like protein IQGAP1 OS=Scoph... [more]
A0A267E8550.000e+097.45Tubulin beta chain (Fragment) OS=Macrostomum ligna... [more]
A0A1I8FZR80.000e+097.91Tubulin beta chain OS=Macrostomum lignano OX=28230... [more]
A0A369S2L50.000e+096.99Tubulin beta chain OS=Trichoplax sp. H2 OX=287889 ... [more]
W4X9L80.000e+098.14Tubulin beta chain OS=Strongylocentrotus purpuratu... [more]
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BLAST of Tubulin beta chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554610.000e+097.21tubulin beta-4B chain [Source:NCBI gene;Acc:103024... [more]
TUBB2A0.000e+094.65tubulin beta-2A chain [Source:NCBI gene;Acc:103041... [more]
TUBB10.000e+079.77tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HG... [more]
ENSAMXT00000019550.20.000e+097.31pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000030346.14.701e-11841.47tubulin alpha-1B chain [Source:NCBI gene;Acc:11119... [more]
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BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb20.000e+093.87tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
TUBA3C2.576e-9743.39tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
tubg14.590e-8233.26tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
ENSPMAT00000009623.17.825e-8284.91pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 ge... [more]
ENSPMAT00000001139.14.891e-7236.75pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
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BLAST of Tubulin beta chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB20.000e+077.16Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB18.696e-11741.61Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB31.362e-11441.61Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB46.498e-4829.32Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin beta chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO399450.000e+097.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359580.000e+096.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO428900.000e+095.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495180.000e+075.93Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO367340.000e+097.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin beta chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb20.000e+095.58tubulin beta-2B chain [Source:NCBI gene;Acc:101162... [more]
tubb50.000e+096.02tubulin beta chain [Source:NCBI gene;Acc:101157969... [more]
ENSORLT00000045809.10.000e+084.19tubulin beta-6 chain [Source:NCBI gene;Acc:1011661... [more]
ENSORLT00000033163.15.806e-11941.94tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
ENSORLT00000020578.25.806e-11941.94tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
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BLAST of Tubulin beta chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000047284.10.000e+099.07SMESG000047284.1[more]
SMESG000046374.10.000e+099.07SMESG000046374.1[more]
SMESG000047291.10.000e+099.07SMESG000047291.1[more]
SMESG000044888.10.000e+099.07SMESG000044888.1[more]
SMESG000035755.10.000e+098.60SMESG000035755.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004118 ID=SMED30004118|Name=Tubulin beta chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1621bp
CCGATCTACAATTTTCTCTTTCTTATTTTACATAGCTACGGTTGCTATGA
CACACAGCAAGTAGGTGTGCATTCTCTACTTGAGTAGTGAACAAATCGAA
TCAGCATTGTAATCTGCTAGCCAGCAGTATAAATAAGAAAGAGAAAATTG
TCATTTTATTACAGTTTTAAGTTAGTAAGAAAAATTTGATTAAAAATGAG
AGAAATTGTTCATATGCAAGCAGGTCAATGTGGAAACCAAATCGGTTCAA
AGTTTTGGGAAGTGATTTCTGATGAGCATGGAATTGATCCGACCGGTACC
TATCATGGAGATTCAGATTTGCAACTTGAAAGAATTAATGTGTATTTTAA
CGAAGCTACTGGAGGCAAATATGTTCCACGTGCTGTGTTGATCGATTTAG
AACCAGGAACAATGGACTCTGTTAGATCGGGGCCGTTTGGTCAATTGTTC
AGACCAGACAATTTCGTTTTTGGACAAAGTGGTGCTGGCAACAACTGGGC
CAAAGGACATTACACTGAAGGAGCAGAATTAGTTGACTCTGTTTTAGATA
TTGTACGAAAGGAAGCTGAATCATGCGATTGTCTTCAAGGATTCCAATTG
ACTCACTCATTGGGTGGAGGAACAGGCTCGGGTATGGGAACACTCCTTAT
TTCTAAAATTAGAGAAGAATACCCAGATCGAATAATGAATACATTTTCGG
TTGTACCATCTCCAAAAGTGTCAGATACCGTCGTTGAACCTTACAATGCT
ACTTTATCCGTCCATCAATTGGTTGAAAATACAGATGAAACATTCTGTAT
CGATAATGAGGCGTTGTACGACATTTGTTTTAGAACATTGAAATTGACAA
CTCCAACCTACGGGGATTTGAATCATTTAGTGAGTGCTACAATGAGTGGA
GTGACAACATGTTTGAGATTCCCTGGTCAATTGAATGCCGATTTGAGAAA
ATTAGCAGTTAACATGGTTCCATTCCCTCGTTTGCATTTCTTTATGCCGG
GATTTGCTCCATTGACCAGTCGAGGAAGTCAACAATATCGTGCATTGACC
GTTCCAGAATTAACTCAACAAATGTTCGATGCCAAGAACATGATGGCTGC
TTGTGATCCAAGACATGGTCGTTATTTGACTGTTGCGGCAATTTTCCGTG
GTCGCATGTCTATGAAAGAAGTTGATGAGCAAATGTTGAATGTTCAAAAT
AAGAATAGTAGTTATTTTGTTGAATGGATTCCCAACAATATTAAAACCGC
GGTTTGTGATATTCCACCAAGAGGATTAAAAATGTCGGCTACATTCATTG
GAAATAGCACTGCTATTCAAGAATTATTCAAGAGAGTGAGTGAACAGTTT
ACAGCTATGTTCCGCAGAAAAGCTTTCCTTCATTGGTACACTGGTGAAGG
TATGGATGAGATGGAGTTCACAGAAGCTGAATCTAATATGAATGACTTGG
TTAGTGAATATCAGCAATATCAAGACGCTACAGCCGAGGAGGAAGGTGAG
TTTGAAGAGGAAGAGGCTGAACAATAGATTTTTCGATTTGAAATTAAATA
AAATTTTGGTTGTTTTTAATTGACATTGTATAACTTTTTTTATAACGTAG
TTTAGTTTTTTTATTTTGGGG
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protein sequence of SMED30004118-orf-1

>SMED30004118-orf-1 ID=SMED30004118-orf-1|Name=SMED30004118-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=444bp
MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVY
FNEATGGKYVPRAVLIDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNN
WAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTL
LISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETF
CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL
RKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIK
TAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTG
EGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEEAEQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000012neural progenitor cell
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0000464GABAergic neuron
PLANA:0000484secretory system
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036525Tubulin/FtsZ_GTPase_sf
IPR037103Tubulin/FtsZ_C_sf
IPR013838Beta-tubulin_BS
IPR002453Beta_tubulin
IPR000217Tubulin
IPR003008Tubulin_FtsZ_GTPase
IPR018316Tubulin/FtsZ_2-layer-sand-dom
IPR023123Tubulin_C
IPR017975Tubulin_CS
IPR008280Tub_FtsZ_C
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005737cytoplasm
GO:0005856cytoskeleton
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0005200structural constituent of cytoskeleton
GO:0003924GTPase activity
GO:0000166nucleotide binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 408..444
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 420..444
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 428..444
NoneNo IPR availablePANTHERPTHR11588:SF340TUBULIN BETA CHAINcoord: 2..440
NoneNo IPR availableCDDcd02187beta_tubulincoord: 2..426
e-value: 0.0
score: 904.63
IPR002453Beta tubulinPRINTSPR01163BETATUBULINcoord: 347..370
score: 79.83
coord: 377..388
score: 83.43
coord: 152..164
score: 90.86
coord: 230..239
score: 83.43
coord: 412..430
score: 93.14
coord: 329..343
score: 89.56
coord: 265..281
score: 85.71
coord: 88..99
score: 94.76
coord: 41..58
score: 81.78
coord: 212..224
score: 85.45
coord: 108..126
score: 83.28
coord: 311..329
score: 78.59
coord: 245..258
score: 89.06
IPR000217TubulinPRINTSPR01161TUBULINcoord: 176..189
score: 79.11
coord: 151..172
score: 57.95
coord: 10..30
score: 72.61
coord: 51..70
score: 71.21
coord: 370..398
score: 67.67
coord: 106..130
score: 68.06
coord: 190..210
score: 69.69
coord: 93..104
score: 76.26
coord: 132..150
score: 79.22
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 2..440
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 246..383
e-value: 2.0E-50
score: 183.5
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 261..382
e-value: 2.3E-43
score: 147.3
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 47..244
e-value: 2.4E-71
score: 253.0
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..211
e-value: 6.8E-70
score: 235.3
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3DG3DSA:3.40.50.1440coord: 1..263
e-value: 4.5E-129
score: 431.6
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILYSSF52490Tubulin nucleotide-binding domain-likecoord: 1..243
IPR023123Tubulin, C-terminalGENE3DG3DSA:1.10.287.600coord: 374..444
e-value: 1.1E-35
score: 123.3
IPR037103Tubulin/FtsZ, C-terminal domain superfamilyGENE3DG3DSA:3.30.1330.20coord: 264..373
e-value: 4.1E-58
score: 196.4
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 140..146
IPR013838Beta tubulin, autoregulation binding sitePROSITEPS00228TUBULIN_B_AUTOREGcoord: 1..4
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILYSSF55307Tubulin C-terminal domain-likecoord: 244..429