SMED30004113

Overview
NameSMED30004113
Smed IDSMED30004113
Length (bp)871
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004113

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30004113 dd_Smed_v4_15124_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30004113 dd_Smed_v4_15124_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30004113 dd_Smed_v4_15124_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30004113 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001499:51936..52798 +4051
Homology
[Close]
BLAST of SMED30004113 vs. TrEMBL
Match: A0A4C1V6U1 (Integrase catalytic domain-containing protein OS=Eumeta variegata OX=151549 GN=EVAR_30697_1 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 4.271e-14
Identity = 43/109 (39.45%), Postives = 56/109 (51.38%), Query Frame = -1 
Query:  422 ICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLPN 748
            I   W ARY     L SDQG+QFES+L   L     I  +R T  +P SNG VER H  LK A+  + N+ +W E+LP +L+ LR    +  N S  +   G    LP 
Sbjct:   86 ILEGWIARYGCPSKLTSDQGKQFESNLFHKLTKLLGIDKIRITPYHPQSNGMVERWHRSLKTALRARLNHKSWIEELPIILLGLRAVPRSDTNVSAAELTFGKTLRLPG 194

HSP 2 Score: 38.1206 bits (87), Expect = 4.271e-14
Identity = 18/37 (48.65%), Postives = 23/37 (62.16%), Query Frame = -2 
Query:  754 ELLKPYKQTKN*QKYLLTIIDLNTNWFEAIPIKQISG 864
            +++ P   T +  KYLLTIID  T W EA P+K IS 
Sbjct:   43 DIVGPLPTTTDDYKYLLTIIDRCTGWPEAFPMKDISA 79
[Close]
BLAST of SMED30004113 vs. TrEMBL
Match: A0A0K8SX59 (Integrase catalytic domain-containing protein (Fragment) OS=Lygus hesperus OX=30085 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 4.314e-14
Identity = 41/105 (39.05%), Postives = 58/105 (55.24%), Query Frame = -1 
Query:  422 WFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLPN 736
            W +RY     + +DQGRQFESDL K LC+   I+       +P SNGK+ER H  LK ++ T+ N  +W   LP +++ LR N ++ +N SP     G    LP 
Sbjct:   77 WISRYGMPTTITTDQGRQFESDLFKSLCTSLGIQHKHTNAYHPQSNGKIERWHRSLKASLSTQINKNDWVGNLPMVMLGLR-NAISGDNTSPASKTFGAPLRLPG 180

HSP 2 Score: 30.8018 bits (68), Expect = 4.314e-14
Identity = 10/24 (41.67%), Postives = 17/24 (70.83%), Query Frame = -2 
Query:  757 QKYLLTIIDLNTNWFEAIPIKQIS 828
            +++ LT ID +TNW E +P+  I+
Sbjct:   42 KRFCLTFIDRSTNWIECLPLADIT 65
[Close]
BLAST of SMED30004113 vs. TrEMBL
Match: A0A4C1UVE8 (Retrovirus-related Pol polyprotein from transposon 412 OS=Eumeta variegata OX=151549 GN=POL PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 5.038e-14
Identity = 45/114 (39.47%), Postives = 59/114 (51.75%), Query Frame = -1 
Query:  410 ICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP-NFTKT 748
            I + W ARY     L SDQG+QFES+L   L     I  +R T  +P SNG VER +  LK A+    N+ +W E+LP +L+ LR    +  N S  +   G    LP NF  T
Sbjct:  424 ILKGWIARYGCPSKLTSDQGKQFESNLFHKLSKFLGIDKIRTTPYHPQSNGMVERWYRSLKSALRAPLNHKSWIEELPIILLGLRAVPQSDTNVSAAELTFGKTLRLPGNFYDT 537

HSP 2 Score: 37.7354 bits (86), Expect = 5.038e-14
Identity = 18/37 (48.65%), Postives = 23/37 (62.16%), Query Frame = -2 
Query:  754 ELLKPYKQTKN*QKYLLTIIDLNTNWFEAIPIKQISG 864
            +++ P   T +  KYLLTIID  T W EA P+K IS 
Sbjct:  381 DIVGPLPTTTDDYKYLLTIIDRCTGWPEAFPMKDISA 417
[Close]
BLAST of SMED30004113 vs. TrEMBL
Match: A0A0A9W9N1 (Gag-Pol polyprotein OS=Lygus hesperus OX=30085 GN=CM83_63276 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 7.721e-14
Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = -1 
Query:  422 NWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLPN 739
            NW +R+    I+ +DQG QF SD+ K  C+   I+ +     +P +NG +ER H  L +AI T ++   W + +  LL+ +R N  +T+  SP Q   G    LPN
Sbjct:  248 NWISRFGAPSIITTDQGTQFMSDIFKKFCAAMGIQNIHTNAYHPQANGMIERFHRTLNEAIMTSSSAAKWAQDMTSLLLGIR-NRPSTDGISPSQRLYGTTPRLPN 352

HSP 2 Score: 34.6538 bits (78), Expect = 7.721e-14
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = -2 
Query:  754 ELLKPYKQTKN*QKYLLTIIDLNTNWFEAIPIKQISG 864
            +LL P+  + N  K+ LTIID  T W E IPI+ I+ 
Sbjct:  203 DLLGPFPAS-NGYKFCLTIIDRATGWPECIPIRNITA 238
[Close]
BLAST of SMED30004113 vs. TrEMBL
Match: A0A0A9WPX9 (Gag-Pol polyprotein OS=Lygus hesperus OX=30085 GN=gag-pol_2 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.856e-13
Identity = 36/106 (33.96%), Postives = 58/106 (54.72%), Query Frame = -1 
Query:  422 NWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLPN 739
            NW +R+    I+ +DQG QF SD+ K  C+   I+ +     +P +NG +ER H  L + I T ++ T W + +  LL+ +R N  +++  SP Q   G+   LPN
Sbjct:  248 NWISRFGAPSIITTDQGTQFMSDIFKKFCAAMGIQNIHTNAYHPQANGMIERFHRTLNETIMTSSSVTKWAQDMTSLLLGIR-NRPSSDGISPSQRLYGVTPRLPN 352

HSP 2 Score: 34.6538 bits (78), Expect = 1.856e-13
Identity = 17/37 (45.95%), Postives = 23/37 (62.16%), Query Frame = -2 
Query:  754 ELLKPYKQTKN*QKYLLTIIDLNTNWFEAIPIKQISG 864
            +LL P+  + N  K+ LTIID  T W E IPI+ I+ 
Sbjct:  203 DLLGPFPAS-NGYKFCLTIIDRATGWPECIPIRNITA 238
[Close]
BLAST of SMED30004113 vs. Ensembl Cavefish
Match: ENSAMXT00000041682.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002320.1:24977:29035:1 gene:ENSAMXG00000038531.1 transcript:ENSAMXT00000041682.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.2326 bits (147), Expect = 4.608e-10
Identity = 40/118 (33.90%), Postives = 53/118 (44.92%), Query Frame = -1 
Query:  425 TNLRHKICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVER----LHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 766
            + + H +   WF  Y   + + SDQGR FESDL K LC  Y +K  R T  +P  NG+ ER    +H LL+           W + LP+LL         T   SP +   G    LP
Sbjct:  982 STVAHILVEKWFYVYGVPQRIHSDQGRNFESDLLKSLCKIYDVKKSRTTPYHPQGNGQCERFNRTMHDLLRTL--PSEQKRRWPKYLPQLLFAYNTTVHQTTAHSPYELMFGRQPRLP 1097
[Close]
BLAST of SMED30004113 vs. Ensembl Cavefish
Match: ENSAMXT00000030702.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:31092205:31094289:-1 gene:ENSAMXG00000035926.1 transcript:ENSAMXT00000030702.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.077 bits (144), Expect = 8.711e-10
Identity = 40/112 (35.71%), Postives = 52/112 (46.43%), Query Frame = -1 
Query:  425 ICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVER----LHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 748
            + R WF +Y   + L SDQGR FES++   LC  Y IK  R T   P  N + ER    LH LL+    +      W E LPEL+    +   +T   SP     G+   LP
Sbjct:  427 LLREWFMKYGVPERLHSDQGRNFESEVIAELCKLYGIKKSRTTPYRPQGNAQCERYNQTLHDLLRTL--SSEKKRRWPEYLPELVYAYNVTPHSTTGYSPYYLLFGVQPRLP 536
[Close]
BLAST of SMED30004113 vs. Ensembl Cavefish
Match: ENSAMXT00000036301.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:28817284:28820114:1 gene:ENSAMXG00000037673.1 transcript:ENSAMXT00000036301.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.447 bits (119), Expect = 9.223e-9
Identity = 32/111 (28.83%), Postives = 54/111 (48.65%), Query Frame = -1 
Query:  425 KICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLH--ILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 751
            ++  ++F R+   + L SDQGR FES +   +C +  ++  R T  +P S+G VER +  +  + AI T  +  +W   LP +L+  R     +   +P Q   G     P
Sbjct:  516 RLVEDFFCRFGIPEELHSDQGRNFESQVMAEVCKRLGVRKTRTTPLHPQSDGLVERFNRTLATQLAITTSRHQKDWDRHLPLVLLSCRAVVQESTGFTPAQLMFGRELRTP 626

HSP 2 Score: 27.7202 bits (60), Expect = 9.223e-9
Identity = 13/47 (27.66%), Postives = 24/47 (51.06%), Query Frame = -2 
Query:  724 ELLKPYKQTKN*QKYLLTIIDLNTNWFEAIPIKQISGIKSAETGSQD 864
            ++L P+  T++  +Y+L  +D  T W EA  +   S   +A    +D
Sbjct:  474 DVLGPFPVTEDGNRYVLVAMDFFTKWPEAYAVPDQSAATTATRLVED 520
[Close]
BLAST of SMED30004113 vs. Ensembl Cavefish
Match: ENSAMXT00000033239.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002121.1:47256:48953:1 gene:ENSAMXG00000036506.1 transcript:ENSAMXT00000033239.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.9062 bits (115), Expect = 2.973e-8
Identity = 30/98 (30.61%), Postives = 49/98 (50.00%), Query Frame = -1 
Query:  461 ICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLH--ILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSP 748
            + R +F R+   + L SDQGR FES++   +C    I   R T  +P S+G VER +  +  + A+ +     +W + LP +L+  R     T   +P
Sbjct:  280 LVREFFCRFGVPEELHSDQGRNFESEVMAEVCRILGIHKTRTTPLHPQSDGLVERFNRTLAAQLAMVSSKGQRDWDKHLPVVLLACRSAVQETTGFTP 377

HSP 2 Score: 27.7202 bits (60), Expect = 2.973e-8
Identity = 11/42 (26.19%), Postives = 22/42 (52.38%), Query Frame = -2 
Query:  739 ELLKPYKQTKN*QKYLLTIIDLNTNWFEAIPIKQISGIKSAE 864
            ++L P+  T +  +Y+L  +D  T W EA  +     + +A+
Sbjct:  237 DVLGPFPVTDSGNRYVLVAMDYFTKWPEAYAVPDQGAVTTAD 278
[Close]
BLAST of SMED30004113 vs. Ensembl Cavefish
Match: ENSAMXT00000041754.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001938.1:55959:59870:1 gene:ENSAMXG00000043385.1 transcript:ENSAMXT00000041754.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 55.4546 bits (132), Expect = 3.064e-8
Identity = 45/142 (31.69%), Postives = 63/142 (44.37%), Query Frame = -1 
Query:  425 LTKIFTHNNRPKYKLVRGNSNQ--TNLRHKICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVER----LHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 832
            LT IFT     KY       +Q  + +   + ++WF R+     + SDQGR FES L K LC  Y+I+  R T+  P  NG+ ER    LH LL+          +W   LP+L          T  ++P     G +  LP
Sbjct:  949 LTDIFT-----KYTQAIATKDQRASTVAWALVQHWFHRFGPPVRIHSDQGRNFESLLIKQLCKVYSIQKSRTTSYRPQGNGQCERFNRTLHDLLRTL--PVEEKRHWPRHLPQLTFAYNTTPHQTTGQTPHFLMFGYHPRLP 1083
[Close]
BLAST of SMED30004113 vs. Ensembl Medaka
Match: ENSORLT00000045127.1 (pep primary_assembly:ASM223467v1:10:587855:593081:1 gene:ENSORLG00000029202.1 transcript:ENSORLT00000045127.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 7.489e-11
Identity = 36/120 (30.00%), Postives = 59/120 (49.17%), Query Frame = -1 
Query:  425 NSNQTNLRHKICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLH--ILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 778
            N    ++   +   W  R+   + + SDQGR FES L K LCS  +I   R +  +P S+G VER +  +L   A+  + N  NW + LP +++  R +   + + +P +   G    LP
Sbjct:   77 NQEAQSIAKVLTEEWVCRFGAPRSIHSDQGRNFESTLFKELCSLLSIHKSRTSPYHPQSDGLVERFNRTLLSLLALFVEDNQLNWDKLLPYVMLAYRSSVHASTSFTPYRVLFGREIVLP 196

HSP 2 Score: 31.9574 bits (71), Expect = 7.489e-11
Identity = 14/32 (43.75%), Postives = 19/32 (59.38%), Query Frame = -2 
Query:  769 ELLKPYKQTKN*QKYLLTIIDLNTNWFEAIPI 864
            E+L P  +T N  KY+L I D  + W EA P+
Sbjct:   44 EILGPLPETPNKNKYILVISDYFSKWTEAFPL 75
[Close]
BLAST of SMED30004113 vs. Ensembl Medaka
Match: ENSORLT00000042487.1 (pep primary_assembly:ASM223467v1:4:23584510:23586585:1 gene:ENSORLG00000030583.1 transcript:ENSORLT00000042487.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 60.077 bits (144), Expect = 7.950e-10
Identity = 39/112 (34.82%), Postives = 54/112 (48.21%), Query Frame = -1 
Query:  425 ICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVER----LHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 748
            + R WF +Y   + L SDQGR FES++ + LC  Y +K  R T  +P  N + ER    LH LL+           W E LPEL+    +    T   SP     G++ +LP
Sbjct:  344 LLREWFMKYGVPERLHSDQGRNFESEVIQELCKLYGVKKTRTTPHHPQGNAQCERFNRTLHDLLRTL--PPEKKRRWPEYLPELIYAYNVTPHATTGYSPYYLLYGVHPHLP 453
[Close]
BLAST of SMED30004113 vs. Ensembl Medaka
Match: ENSORLT00000044989.1 (pep primary_assembly:ASM223467v1:1:17572028:17576089:-1 gene:ENSORLG00000028051.1 transcript:ENSORLT00000044989.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.3066 bits (142), Expect = 1.773e-9
Identity = 39/112 (34.82%), Postives = 54/112 (48.21%), Query Frame = -1 
Query:  425 ICRNWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVER----LHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 748
            + R WF +Y   + L SDQGR FES++ + LC  Y +K  R T  +P  N + ER    LH LL+           W E LPEL+    +    T   SP     G++ +LP
Sbjct:  969 LLREWFMKYGVPERLHSDQGRNFESEVIQELCKLYGVKKTRTTPHHPQGNAQCERFNRTLHDLLRTL--PPEKKRRWPEYLPELIYAYNVTPHATTGYSPYYLLYGVHPHLP 1078
[Close]
BLAST of SMED30004113 vs. Ensembl Medaka
Match: ENSORLT00000036827.1 (pep primary_assembly:ASM223467v1:16:28613823:28617539:1 gene:ENSORLG00000023550.1 transcript:ENSORLT00000036827.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.5362 bits (140), Expect = 2.853e-9
Identity = 32/82 (39.02%), Postives = 51/82 (62.20%), Query Frame = -1 
Query:  494 FARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLH-ILLKQ-AI*TKANNTNWYEKLPELLIRLR 733
            F+R+   +++ SDQGR FES +   +C +  ++  R T  +P S+G VER +  L+KQ AI T A+ ++W E LP +L+  R
Sbjct:  935 FSRFGAAEVIHSDQGRNFESAVFSAMCERLGMRKTRTTPLHPQSDGLVERFNRTLVKQLAILTSAHQSDWDEHLPLVLMAYR 1016
[Close]
BLAST of SMED30004113 vs. Ensembl Medaka
Match: ENSORLT00000032171.1 (pep primary_assembly:ASM223467v1:18:2114932:2117958:1 gene:ENSORLG00000025397.1 transcript:ENSORLT00000032171.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.9138 bits (128), Expect = 8.514e-8
Identity = 37/108 (34.26%), Postives = 51/108 (47.22%), Query Frame = -1 
Query:  425 WFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVER----LHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSPCQS*LGLNRNLP 736
            WF+++     L SDQGR FES L + LC  Y I+  R T  +P  NG+ ER    LH LL+    T+    +W+  LP++          T  +SP     G    LP
Sbjct:  897 WFSKFGVPARLHSDQGRSFESQLIQQLCGLYGIEKSRTTPYHPAGNGQCERFNRTLHNLLRALQVTRKR--DWHSCLPQVTFCYNTTPHQTTGESPFFFMFGQQPRLP 1002
[Close]
BLAST of SMED30004113 vs. Planmine SMEST
Match: SMESG000000071.1 (SMESG000000071.1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.125e-18
Identity = 44/93 (47.31%), Postives = 61/93 (65.59%), Query Frame = -1 
Query:  461 NWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSP 739
            NW  RY + K +++DQ RQFES L + LC  Y IK  R T  NP +NGK+ERLH  LK+++ TKA + NW  + P +L+ LR    +T+N+SP
Sbjct:  266 NWIGRYGQPKTIMTDQVRQFESQLFEMLCKIYNIKRNRTTAFNPQANGKIERLHKTLKESLRTKAEDRNWLNQPPTILLGLRNTPNSTDNESP 358
[Close]
BLAST of SMED30004113 vs. Planmine SMEST
Match: SMESG000007179.1 (SMESG000007179.1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.116e-15
Identity = 42/93 (45.16%), Postives = 58/93 (62.37%), Query Frame = -1 
Query:  461 NWFARYERTKILISDQGRQFESDLSKGLCSKYTIKTVRPTT*NP*SNGKVERLHILLKQAI*TKANNTNWYEKLPELLIRLRINHLNTNNKSP 739
            NW ARY++ K +I+DQ RQFE      LC  Y  K  R T  NP +NGK+ERLH  LK+++ TKA + NW  +LP +L   R    +T+N++P
Sbjct:  233 NWIARYDQPKTIITDQERQFEM-----LCKNYNFKRNRITAYNPQANGKIERLHRTLKESLRTKAEDRNWLNQLPTILPGQRNTPNSTDNETP 320
The following BLAST results are available for this feature:
BLAST of SMED30004113 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4C1V6U14.271e-1439.45Integrase catalytic domain-containing protein OS=E... [more]
A0A0K8SX594.314e-1439.05Integrase catalytic domain-containing protein (Fra... [more]
A0A4C1UVE85.038e-1439.47Retrovirus-related Pol polyprotein from transposon... [more]
A0A0A9W9N17.721e-1434.91Gag-Pol polyprotein OS=Lygus hesperus OX=30085 GN=... [more]
A0A0A9WPX91.856e-1333.96Gag-Pol polyprotein OS=Lygus hesperus OX=30085 GN=... [more]
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BLAST of SMED30004113 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000041682.14.608e-1033.90pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000030702.18.711e-1035.71pep primary_assembly:Astyanax_mexicanus-2.0:16:310... [more]
ENSAMXT00000036301.19.223e-928.83pep primary_assembly:Astyanax_mexicanus-2.0:15:288... [more]
ENSAMXT00000033239.12.973e-830.61pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000041754.13.064e-831.69pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of SMED30004113 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004113 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000045127.17.489e-1130.00pep primary_assembly:ASM223467v1:10:587855:593081:... [more]
ENSORLT00000042487.17.950e-1034.82pep primary_assembly:ASM223467v1:4:23584510:235865... [more]
ENSORLT00000044989.11.773e-934.82pep primary_assembly:ASM223467v1:1:17572028:175760... [more]
ENSORLT00000036827.12.853e-939.02pep primary_assembly:ASM223467v1:16:28613823:28617... [more]
ENSORLT00000032171.18.514e-834.26pep primary_assembly:ASM223467v1:18:2114932:211795... [more]
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BLAST of SMED30004113 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000000071.11.125e-1847.31SMESG000000071.1[more]
SMESG000007179.12.116e-1545.16SMESG000007179.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004113 ID=SMED30004113|Name=SMED30004113|organism=Schmidtea mediterranea sexual|type=transcript|length=871bp
TGTGTTTAACAACAATAATAGGAAAAAAATATAAAAAATGTAAAACGTTC
ATTTGTTTGTATATAGTTGATTTTAAATTATTTATTTATGCAGGTTTGTT
TGAAAAAGTCACTGTACATTTTGTTTTTTGGAGATGTTTTGATTGCAATT
ACGTCGTTGAATAAAATGTGAATTTGTTTTTGTGTTTGGTTGGTGGTTAT
TGGTTTATTGTTTGTTCGGTAAGGTTTTAATCTGCAAACGTTGATTCTGA
TTTTTCTGTTATTTACAAAAAGTTATTTTTCTAATAGTTTGTATAGACCA
TCATGTTTTGGTTCAAAAGCTTTTGTGAATGGTCTCTTGATAATTTCTTT
TAAATCATTTTCCTTTTTTATTATTTTGCCTGTCTGATTTTCTGTCGAAT
TTTGAAAAGAGTTTTTGTAAAATTTGGTAAGTTTCTGTTGAGTCCTAACT
ATGATTGACAAGGACTTTTATTATTTGTATTTAAGTGATTAATTCTTAGT
CTTATTAATAATTCTGGTAGTTTCTCATACCAGTTTGTGTTGTTCGCTTT
TGTTCATATTGCTTGTTTGAGTAATATGTGTAGTCTTTCTACTTTCCCAT
TTGATTAAGGGTTTTATGTCGTAGGTCTGACTGTTTTAATTGTATATTTT
GAACACAGTCCTTTGGATAAGTCAGATTCGAACTGCCTACCTTGATCGCT
GATAAGTATTTTTGTTCGTTCGTATCTTGCGAACCAGTTTCTGCAGATTT
TATGCCTGAGATTTGTTTGATTGGAATTGCCTCGAACCAGTTTGTATTTA
GGTCGATTATTGTGAGTAAATATTTTTGTTAATTTTTTGTTTGCTTATAT
GGTTTTAGTAATTCTTTTTAG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding