Acid-sensing ion channel 4

Overview
NameAcid-sensing ion channel 4
Smed IDSMED30004107
Length (bp)1543
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Acid-sensing ion channel 4 (SMED30004107) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Acid-sensing ion channel 4 (SMED30004107) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004107

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30004107h1SMcG0005025 SmedASXL_019886SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30004107h1SMcG0005025 SmedASXL_007856SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30004107h1SMcG0005025 dd_Smed_v4_31940_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30004107h1SMcG0005025 dd_Smed_v4_31940_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Acid-sensing ion channel 4 vs. TrEMBL
Match: A0A1S6KMI4 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 5.209e-12
Identity = 90/364 (24.73%), Postives = 157/364 (43.13%), Query Frame = 3 
Query:  426 KAAVLVDPRIPLVKCFMDNEYDCR-VSFSKVFIYENRPCIS-----GNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPN----GLAL-EAVKNQY------FLNINYELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLL--DASNSTVESIIERVKCHDKL-INNMSYSCPQKCN---YYDVSVFSQ-----------YSQLKTTTE-VLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFKLLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKMAFLSEIGGIMGLYMGWSAIT 1412
            K+   VDP +P+ KC   ++ +CR +     F+  N+ C +      N +   I S  I   W  S L    S  +  ++ F++VY+H     P+    G A+ +   N Y      F+N+    S  R  C +V +++++ S  + +I +  YS+ LC  I+ Q  ++  C CIS  L IP  F   +  N + +CH I   LL        +  +  ++ C++ +  N+   SC Q CN   Y   + F Q           +S++ T TE  ++N+    +  K+T                  +   LN        L+  I+   E+  F   +++  +  LS++GGI+GLY G   +T
Sbjct:    2 KSGAFVDPVLPVFKCVTYSDGNCRQILKHSRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSG-WPTWDNFVSVYIHGKWQLPSSTTAGYAIPDTTGNAYIYLQKTFINME---STKRNPCLNVADNVEI-SDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIYWSEIPTITEPYIRNYFYYPDCFKTT------------------ISAKLNCKIVKSNVLKFHINLIGEIKTFSESSSYSIVNLLSDVGGILGLYGGSGCLT 342
[Close]
BLAST of Acid-sensing ion channel 4 vs. TrEMBL
Match: A0A267G3Q5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003120g3 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.226e-9
Identity = 104/460 (22.61%), Postives = 182/460 (39.57%), Query Frame = 3 
Query:  144 HFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMPFFIQNFS-NPVPR-FNSKMNFSVVDMLTMEFWDRNLNLINVPYGDLF---KIYFHSIIPLADKAAVLVDPRIPLVKC-------FMDNEYDCRVSFSKVFIYENRPCISGNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPNGLALEAVKNQY--FLNINY--ELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLLDASNST----VESIIERVKCHDKLINNMSYS-----------CPQKCN--YYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFKLLNDLNINSP--TEAFLQIGIDYSPEVLMFESRNAFDKMA--FLSEIGGIMGLYMGWSAIT 1412
            H  +L  +F   P++T+I        +P I+IC    F ++ +  NP  R  N  +   V D+ +  FWD N    +   GDL    K Y H +        +++        C       ++D  Y    +FS       R  + G      ++     I +    +  I+    + Y+      +H  GT P  L    V+     ++ + Y  E+   +    +  + ++ + R   ++  + YS   C     Q  +  +C C+SE LP+P  F  I  +N+  +CH I    L  S         S+ E+ +CHD  IN +              CP KCN   Y++ V     Q K   +  +    L+   ++T  T  + R   DS    + + +   N P   + F ++ + YS   +    R  +  +A    S+IGGI+GL++G S I+
Sbjct:   82 HLISLFSRFNEGPVNTEISVYSTNFVFPDISICATSHFSLRRYERNPKIRSLNKPIKELVSDIRS--FWDPNNESDSEINGDLLNSLKSYLHEVNITKQSQEMILHCDFAGTICSYINFTEYIDPNYFSCFTFSP----RERHLLVGGERDGGLS----VIMYVGDMMNFISEEDSLRYSKGFRAVIHEKGTRPEMLEGFYVQEHACTYVQLGYTEEVVLRKCAEEESLQPLEFVDRYG-KVTQYRYSVRDCQVNLYQNFLNESCRCVSEILPLPAKFRNITRHNI-TFCHHINEERLKQSRGAKSNDSSSVREKQECHDNAINEIHKQVSAKKTKKDSYCPLKCNNTRYEMRVM----QNKWPNDANETRQALW---EATQRTMHEARSRGDSTEVLEEIMEHLKNDPYALQRFAKLTV-YSHSTMGNIVRENWAYLARNLASDIGGILGLWLGVSIIS 521
[Close]
BLAST of Acid-sensing ion channel 4 vs. TrEMBL
Match: A0A267EAK0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003120g1 PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 9.545e-7
Identity = 97/434 (22.35%), Postives = 170/434 (39.17%), Query Frame = 3 
Query:  222 YPSITICYNMPFFIQNFS-NPVPR-FNSKMNFSVVDMLTMEFWDRNLNLINVPYGDLF---KIYFHSIIPLADKAAVLVDPRIPLVKC-------FMDNEYDCRVSFSKVFIYENRPCISGNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPNGLALEAVKNQY--FLNINY--ELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLLDASNST----VESIIERVKCHDKLINNMSYS-----------CPQKCN--YYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFKLLNDLNINSP--TEAFLQIGIDYSPEVLMFESRNAFDKMA--FLSEIGGIMGLYMGWSAIT 1412
            +P I+IC    F ++ +  NP  R  N  +   V D+ +  FWD N    +   GDL    K Y H +        +++        C       ++D  Y    +FS       R  + G      ++     I +    +  I+    + Y+      +   GT P  L    V+     ++ + Y  E+   +    +  + ++ + R   ++  + YS   C     Q  +  +C C+SE LP+P  F  I  +N+  +CH I    L  S         S+ E+ +CHD  IN +              CP KCN   Y++ V     Q K   +  +    L+   ++T  T  + R   DS    + + +   N P   + F ++ + YS   +    R  +  +A    S+IGGI+GL++G S I+
Sbjct:  108 FPDISICATSHFSLRRYERNPKIRSLNKPIKELVSDIRS--FWDPNNESDSEINGDLLNSLKSYLHEVNITKQSQEMILHCDFAGTICSYINFTEYIDPNYFSCFTFSP----RERHLLVGGERDGGLS----VIMYVGDMMNFISEEDSLRYSKGFRAVIQEKGTRPEMLEGFYVQEHACTYVQLGYTEEVVLRKCAEEESLQPLEFVDRYG-KVTQYRYSVRDCQVNLYQNFLNESCRCVSEILPLPAKFRNITRHNI-TFCHHINEERLKQSRGAKSNDSSSVREKQECHDNAINEIHKQVSAKKTKKDSYCPLKCNNTRYEMRVM----QNKWPNDANETRQALW---EATQRTMHEARSRGDSTEVLEEIMEHLKNDPYALQRFAKLTV-YSHSTMGNIVRENWAYLARNLASDIGGILGLWLGVSIIS 521
[Close]
BLAST of Acid-sensing ion channel 4 vs. Planmine SMEST
Match: SMESG000053471.1 (SMESG000053471.1)

HSP 1 Score: 941.414 bits (2432), Expect = 0.000e+0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 3 
Query:    3 SSAAVVEHFLETCSAHAYCKLTSHYKNKYRKGXXXXXXXXXXXXXXYHFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMPFFIQNFSNPVPRFNSKMNFSVVDMLTMEFWDRNLNLINVPYGDLFKIYFHSIIPLADKAAVLVDPRIPLVKCFMDNEYDCRVSFSKVFIYENRPCISGNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPNGLALEAVKNQYFLNINYELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLLDASNSTVESIIERVKCHDKLINNMSYSCPQKCNYYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFKLLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKMAFLSEIGGIMGLYMGWSAITXXXXXXXXXXXXXYHFIAY 1469
            SSAAVVEHFLETCSAHAYCKLTSHYKNKYRKGIWIFILILSCIIFIYHFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMPFFIQNFSNPVPRFNSKMNFSVVDMLTMEFWDRNLNLINVPYGDLFKIYFHSIIPLADKAAVLVDPRIPLVKCFMDNEYDCRVSFSKVFIYENRPCISGNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPNGLALEAVKNQYFLNINYELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLLDASNSTVESIIERVKCHDKLINNMSYSCPQKCNYYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFKLLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKMAFLSEIGGIMGLYMGWSAITVIEIIVFFVELIVYHFIAY
Sbjct:    2 SSAAVVEHFLETCSAHAYCKLTSHYKNKYRKGIWIFILILSCIIFIYHFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMPFFIQNFSNPVPRFNSKMNFSVVDMLTMEFWDRNLNLINVPYGDLFKIYFHSIIPLADKAAVLVDPRIPLVKCFMDNEYDCRVSFSKVFIYENRPCISGNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPNGLALEAVKNQYFLNINYELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLLDASNSTVESIIERVKCHDKLINNMSYSCPQKCNYYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFKLLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKMAFLSEIGGIMGLYMGWSAITVIEIIVFFVELIVYHFIAY 490
[Close]
BLAST of Acid-sensing ion channel 4 vs. Planmine SMEST
Match: SMESG000032616.1 (SMESG000032616.1)

HSP 1 Score: 120.553 bits (301), Expect = 4.771e-29
Identity = 132/493 (26.77%), Postives = 222/493 (45.03%), Query Frame = 3 
Query:   24 HFLETCSAHAYCKLTSHYKNKYRKGXXXXXXXXXXXXXXYHFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMP-FFIQNFSNPVPRFNSKMNFSVVDMLTMEFWDRNLNLINVPY----GDLFKIYFHSIIPLADKAAVLVDPRIPLVKCFMDNEYDCR-VSFSKVFIYENRPCIS-----GNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPN----GLAL-EAVKNQY------FLNINYELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLL--DASNSTVESIIERVKCHDKL-INNMSYSCPQKCN---YYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFK--LLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKMAFLSEIGGIMGLYMGWSAIT 1412
             FLET +AH + +L      K     WI ++ILS + F YH Y L+L++   P+STK+ +      +PS+TIC++ P  +  N +N +P +N+  +F  V  L  EFW         PY      +  +   S+IP   K+   VDP +P+ KC   ++ +CR +     F+  N+ C +      N +   I S  I   W  S L    S  +  ++ F++VY+H     P+    G A+ +   N Y      F+N+    S  R  C DV +++++ S  + +I +  YS+ LC  I+ Q  ++  C CIS  L IP  F   +  N + +CH I   LL        +  +  ++ C++ +  N+   SC Q CN   Y   + F Q++      ++         + +    T    R +F     FK  +   LN        L+  I+   E+      +++  +  LS++GGI+GLY G   +T
Sbjct:   17 EFLETTTAHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNN--SFQNVSSLVREFWSS-------PYVNQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHSRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSG-WPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINME---STKRNPCLDVADNVEI-SDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCNNAIYNGNNYFYQFNPPTYEKDIY--------WSEIPTITEPYIRNYFYYPDCFKTTISAKLNCKIVKSNVLKFHINLIGEIKTSSESSSYSIVNLLSDVGGILGLYGGSGCLT 487
[Close]
BLAST of Acid-sensing ion channel 4 vs. Planmine SMEST
Match: SMESG000032618.1 (SMESG000032618.1)

HSP 1 Score: 116.316 bits (290), Expect = 6.731e-28
Identity = 109/379 (28.76%), Postives = 179/379 (47.23%), Query Frame = 3 
Query:   27 FLETCSAHAYCKLTSHYKNKYRKGXXXXXXXXXXXXXXYHFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMP-FFIQNFSNPVPRFNSKMNFSVVDMLTMEFWDRNLNLINVPY----GDLFKIYFHSIIPLADKAAVLVDPRIPLVKCFMDNEYDCR-VSFSKVFIYENRPCIS-----GNVSMPKITSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGTYPN----GLAL-EAVKNQY------FLNINYELSYNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLL--DASNSTVESIIERVKCHDKL-INNMSYSCPQKCN 1088
            FLET +AH + +L      K     WI ++ILS + F YH Y L+L++   P+STK+ +      +PS+TIC++ P  +  N +N +P +N+  +F  V  L  EFW         PY      +  +   S+IP   K+   VDP +P+ KC   ++ +CR +     F+  N+ C +      N +   I S  I   W  S L    S  +  ++ F++VY+H     P+    G A+ +   N Y      F+N+    S  R  C DV +++++ S  + +I +  YS+ LC  I+ Q  ++  C CIS  L IP  F   +  N + +CH I   LL        +  +  ++ C++ +  N+   SC Q CN
Sbjct:   18 FLETTTAHGFIRLVLFKHRKIVITFWIVVIILSIVAFAYHLYVLSLRYTGVPLSTKLAFSPFNQDWPSVTICFDNPNLYGGNSTNYIPYYNN--SFQNVSSLVREFWSS-------PYVNQTDQISLLSLQSLIPFGMKSGAFVDPVLPVFKCVTYSDGNCRQILKHSRFVMNNKICATVDLQESNTTNNVIHSFSIIYLWMSSTLNTYVSG-WPTWDNFVSVYIHGKWQLPSSSTAGYAIPDTTGNAYIYLQKTFINME---STKRNPCLDVADNVEI-SDFDGQIRSLNYSQQLCKMINIQNQLWDKCQCISSNLLIPGRFSRHLNENQNIFCHHIHSNLLLNMTDFQHLNQLKAKLSCYENVYFNSPVDSCIQYCN 382
[Close]
BLAST of Acid-sensing ion channel 4 vs. Planmine SMEST
Match: SMESG000079503.1 (SMESG000079503.1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.308e-11
Identity = 114/459 (24.84%), Postives = 180/459 (39.22%), Query Frame = 3 
Query:  141 YHFYTLALKFMSNPISTKIKYDFQIDTYPSITICYNMPFF-IQNFSNPVPRFNSKMNFSVVDMLTMEFWDRNLNLINVPYGDLFKIYFHSIIPLADKAAVLVDPRIPLVKCFMDNEYDCRVSFSKVFIYENRPCISGNVSMPKI-TSIKIAIFWPYSFLYPINSNPFINYNTFLAVYLHPLGT----------YPNGLALEAVKNQYFL---NINYELSYNRLVCNDVF---EDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLLD-----------ASNSTVESIIERVKCHDKLINNM--SYSCPQKCNYYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQ-----SYFKLLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKMAFLSEIGGIMGLYMGWSAI 1409
            +HF T+ + F+++ + T++  +      P++ IC + P+  +     PV  FN  M  S        FW       N+     F     SI P A +  +        V C  +N   C VS     I +   C   ++ +    TS+     W YSF    +  P  N   + + Y++ L T          YPN  +      + F+   N+N  +    LV N      E +K+LS  N  IL F+Y+KSLC  +  Q  IF  C C SE LPIPN    I  + V  +CH +  G ++            +N     +     CHD  +N+     SC   C     S +S  + +   T    N  N        +N N+K  ++  ++     S  K  +D+  N    +F    +  +P  L       +    FLS IG I+ L++G S I
Sbjct:   78 FHFITMLMIFLAHEVQTEMVNNAVPFEMPTVIICPDAPYAKLFKVDRPV-IFN--MTLSGAFDTFNAFWKLVNKTTNIQEKSRF-----SIAPYAQEYQLFTLSEDLTVFCKYNNAVKCNVS--NFMIEDLSVCAKIDIPLDTTKTSLSQLNVW-YSFFTDASYLP--NTTFYNSKYMYILRTAYLTYQAKEEYPNFHSTRISGFKQFMTTINLNIRIQNEFLVKNSCLSNPEPMKILSYGN-AILQFSYTKSLCLMVKKQNYIFQNCKCFSEALPIPNYSDNITIDIVP-FCHKLSKGFIEYLKTIPNPINILNNKETAQLTSSSVCHDHFLNSWIDDGSCISPCTSKSYS-WSNINAVSWPTVTFTNTPNKILISDIQSNINQKMAKYNVTENDLVYSTIKTTSDIISNRKLGSFKI--VPQTPSGLKITESENYPLTNFLSTIGSILVLWVGISII 518
[Close]
BLAST of Acid-sensing ion channel 4 vs. Planmine SMEST
Match: SMESG000019202.1 (SMESG000019202.1)

HSP 1 Score: 59.6918 bits (143), Expect = 3.597e-9
Identity = 77/300 (25.67%), Postives = 132/300 (44.00%), Query Frame = 3 
Query:  657 AVYLHPLGTYPNGLALEAVKNQYFLNINYELSYNRL---------VCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCISETLPIPNDFYGIVYNNVDNYCHAIWGGLLDASNSTV--------ESIIERVK----CHD---KLINNMSY------SCPQKCNYYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNKKQRQFFDSQSYFKLLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKMAFLSEIGGIMGLYMGWSAITXXXXXXXXXXXXXYHFIA 1466
             +Y+H   T PN    ++  +  + ++  + S+N+          VC D   DI +     +E+ T+TYS+SLC +     +I+ TCNC SE L         V  +  ++CH I   L    N++           +I  +K    CH     L+ N  +      +C +    Y+VS      QL     V    +N    L    N   K  +   + + ++LL D           Q+ ++ + E L +   N       LS+IGGI+GLY+G+S ++++EI   FV+LI+++FI+
Sbjct:  264 TLYMHSPNTMPN---FKSDGHMVYRSLVKQFSFNQAKIIRENSGSVCFDKPNDIVIKGYGLNEMNTYTYSQSLCVETKKSEMIYKTCNCRSELL-----IASSVIPDSASFCHEISSLLSTKLNNSFIMLNPKDNTDVIANLKKNYLCHKTQISLLENEKFPETCLPACVESTYRYEVSSSKLERQLIGFKGVYNESINEIIPLLQQEN---KSVEVIKAAANYELLTDFKFTVSKFIPKQLTVEVTEESLSYPIEN------LLSDIGGILGLYLGFSVMSMLEIAELFVQLIIHNFIS 546
The following BLAST results are available for this feature:
BLAST of Acid-sensing ion channel 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A1S6KMI45.209e-1224.73Uncharacterized protein OS=Schmidtea mediterranea ... [more]
A0A267G3Q51.226e-922.61Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267EAK09.545e-722.35Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acid-sensing ion channel 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000053471.10.000e+0100.00SMESG000053471.1[more]
SMESG000032616.14.771e-2926.77SMESG000032616.1[more]
SMESG000032618.16.731e-2828.76SMESG000032618.1[more]
SMESG000079503.12.308e-1124.84SMESG000079503.1[more]
SMESG000019202.13.597e-925.67SMESG000019202.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004107 ID=SMED30004107|Name=Acid-sensing ion channel 4|organism=Schmidtea mediterranea sexual|type=transcript|length=1543bp
TGTCTAGCGCTGCAGTCGTAGAGCATTTCCTAGAGACTTGTTCGGCTCAT
GCCTACTGCAAGCTCACCAGTCATTATAAAAATAAATATAGGAAAGGAAT
TTGGATCTTCATTTTAATTTTGTCCTGCATTATTTTCATTTACCATTTTT
ATACTTTGGCCTTGAAATTTATGAGCAATCCGATCTCAACGAAGATCAAG
TACGATTTCCAGATAGACACTTACCCATCAATTACTATTTGTTACAATAT
GCCGTTTTTTATTCAAAATTTTTCTAATCCAGTTCCTCGATTTAACTCCA
AAATGAATTTCTCTGTCGTTGACATGCTAACGATGGAATTCTGGGACAGA
AATTTGAACTTGATAAATGTCCCATACGGTGATCTGTTCAAGATTTACTT
CCATTCAATTATCCCGCTGGCAGATAAAGCTGCAGTTCTGGTTGATCCTC
GTATTCCATTGGTCAAATGCTTCATGGACAACGAATATGATTGTCGTGTT
TCATTTTCAAAGGTTTTTATTTATGAGAACCGTCCCTGTATTAGTGGGAA
TGTTTCCATGCCGAAAATTACTAGCATTAAAATAGCAATTTTTTGGCCTT
ATTCGTTCTTGTATCCGATAAACAGTAATCCTTTTATAAATTACAATACG
TTCCTTGCAGTTTATCTGCATCCTCTAGGAACTTATCCAAACGGTTTGGC
TTTGGAAGCAGTAAAAAATCAGTACTTTTTGAATATTAATTATGAGCTAT
CCTACAACCGATTGGTGTGTAATGACGTCTTTGAAGATATAAAGGTGTTA
TCAAGACTGAATGATGAAATACTAACTTTTACGTATTCAAAATCTTTGTG
CGATCAAATATCCACTCAAAGAATAATCTTTGCTACTTGTAATTGTATTT
CAGAAACTCTCCCGATCCCCAATGATTTCTATGGAATAGTCTACAATAAT
GTTGATAACTATTGTCATGCTATATGGGGAGGACTGTTAGACGCCTCCAA
CAGCACAGTTGAGTCAATTATCGAAAGAGTGAAATGCCATGACAAGTTGA
TTAACAACATGAGCTATTCGTGTCCCCAGAAGTGCAATTACTATGATGTA
TCAGTTTTCTCCCAATACTCACAACTGAAAACCACAACTGAAGTTTTAAA
AAACTTTGTAAATTTATACAATTACTTAAAATCAACAAACAACACAAACA
AAAAGCAAAGGCAATTTTTTGATTCCCAATCGTATTTTAAACTTCTCAAT
GATTTGAACATCAACAGCCCAACGGAAGCCTTTTTACAAATTGGAATTGA
CTATTCTCCAGAGGTCTTGATGTTTGAAAGTCGAAACGCATTTGATAAAA
TGGCTTTCTTGTCAGAAATCGGAGGAATTATGGGACTTTATATGGGTTGG
AGCGCGATCACAGTCATTGAAATAATTGTCTTCTTCGTTGAATTAATTGT
CTATCATTTTATTGCTTATTAAACTTTTTGTTACTTAGAAATAAGACTGT
ACTCCTTCTTGTCAAGGAGAAATTGAATCTTCATCTTTATTAC
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protein sequence of SMED30004107-orf-1

>SMED30004107-orf-1 ID=SMED30004107-orf-1|Name=SMED30004107-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=490bp
SSAAVVEHFLETCSAHAYCKLTSHYKNKYRKGIWIFILILSCIIFIYHFY
TLALKFMSNPISTKIKYDFQIDTYPSITICYNMPFFIQNFSNPVPRFNSK
MNFSVVDMLTMEFWDRNLNLINVPYGDLFKIYFHSIIPLADKAAVLVDPR
IPLVKCFMDNEYDCRVSFSKVFIYENRPCISGNVSMPKITSIKIAIFWPY
SFLYPINSNPFINYNTFLAVYLHPLGTYPNGLALEAVKNQYFLNINYELS
YNRLVCNDVFEDIKVLSRLNDEILTFTYSKSLCDQISTQRIIFATCNCIS
ETLPIPNDFYGIVYNNVDNYCHAIWGGLLDASNSTVESIIERVKCHDKLI
NNMSYSCPQKCNYYDVSVFSQYSQLKTTTEVLKNFVNLYNYLKSTNNTNK
KQRQFFDSQSYFKLLNDLNINSPTEAFLQIGIDYSPEVLMFESRNAFDKM
AFLSEIGGIMGLYMGWSAITVIEIIVFFVELIVYHFIAY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006811ion transport
GO:0035725sodium ion transmembrane transport
GO:0006814sodium ion transport
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR001873ENaC
Vocabulary: molecular function
TermDefinition
GO:0005272sodium channel activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001873Epithelial sodium channelPRINTSPR01078AMINACHANNELcoord: 457..473
score: 45.41
coord: 31..48
score: 13.33
coord: 443..457
score: 20.8
coord: 70..86
score: 29.88
IPR001873Epithelial sodium channelPFAMPF00858ASCcoord: 9..477
e-value: 1.8E-30
score: 106.3
IPR001873Epithelial sodium channelPANTHERPTHR11690AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATEDcoord: 14..480
NoneNo IPR availableGENE3DG3DSA:1.10.287.820coord: 274..361
e-value: 9.6E-13
score: 49.8
NoneNo IPR availableGENE3DG3DSA:2.60.470.10coord: 362..445
e-value: 9.6E-13
score: 49.8
NoneNo IPR availableGENE3DG3DSA:1.10.287.770coord: 446..475
e-value: 9.6E-13
score: 49.8
NoneNo IPR availableTMHMMTMhelixcoord: 462..484
NoneNo IPR availableTMHMMTMhelixcoord: 32..54