SMED30004099

Overview
NameSMED30004099
Smed IDSMED30004099
Length (bp)2243
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30004099 (SMED30004099) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30004099 (SMED30004099) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30004099 (SMED30004099) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30004099 (SMED30004099) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004099

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30004099SMESG000058734.1 SMESG000057289.1 SMESG000076438.1 SMESG000071011.1 SMESG000052064.1 SMESG000039181.1 SMESG000038459.1 SMESG000037745.1 SMESG000034409.1 SMESG000030447.1 SMESG000003965.1 SMESG000065288.1 SMESG000065178.1 SMESG000062279.1 SMESG000061056.1 SMESG000048922.1 SMESG000029226.1 Contig50916uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30004099SMESG000058734.1 SMESG000057289.1 SMESG000076438.1 SMESG000071011.1 SMESG000052064.1 SMESG000039181.1 SMESG000038459.1 SMESG000037745.1 SMESG000034409.1 SMESG000030447.1 SMESG000003965.1 SMESG000065288.1 SMESG000065178.1 SMESG000062279.1 SMESG000061056.1 SMESG000048922.1 SMESG000029226.1 Contig50916newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30004099 dd_Smed_v4_15498_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30004099 vs. Planmine SMEST
Match: SMESG000035043.1 (SMESG000035043.1)

HSP 1 Score: 731.095 bits (1886), Expect = 0.000e+0
Identity = 418/593 (70.49%), Postives = 445/593 (75.04%), Query Frame = 2 
Query:  395 AVSPDKNRLAMVDEPEACNEVLAVLPEKNRCNMVSGPEAVKATSSTRDVDGKAEIPVGRRELGKSPRKEIIEKCAALVKYRDQWESXXXXXXXXXXXXXXXXXXXXXXXGIIATLERDEDYYYLIKNLMRKIGASPRYANETSEAVVITVLPGKMLHRFEIDPKETVGELVKLLKEARPGGNYVLYKYGAEMLEGDTLLSYGILPNYNNTVDVVYPTEKVKVIDTDNVFMITEMEIEQLNQANAIPQRLGFELMQTPRDGEAIKGIMDWMLELRIPVNTLRDIMPLVLPLLKVTSPFQAQAKLLCIKNGHAIRHETSRQGPLWLEIRIPGRGKLKQQCSCFHKVRKLRERLSEILQGLFLIVIHGCQVDDDEFLVDVGCFPREETQVLIVPIELPREPWSIMRLPDCQEMESEPVQVELPKGEFAPLPPYPVDLDEEIERFLENYDQPTEGENREVDTERVFGQQIIEDERDTIIQLDPVETDVGASTSREPDPPARPGIIQSEIKVKKDGRGRPKKSQMKPPPNEQLNPETIQNLTRQALAGIAKQIDDDSENHPTFKSMM*RRLQQIMRLLKQDWQIPKVKQLRALRLSTD 2173
            AVSP KNR AM +EPEACNEVLAVLP+KN CNMVS PEA KATSSTR+VDGKAEIP GRRE G+S R+E IEKCAALVKYRDQWESEVVEKL +IDKEEIEEAEIDKILGI+ATLE D+DYYYLIKNLMRKIGASPRY                                      A   G  V++     +L G  LL + I P    TV      E VK+                L +ANAIPQRLGFELMQTPRDGEAIKGIMDWMLELRIPVNTLRDIMPLVLPLLKVTS FQAQAKLLCIK GHAIRHE SRQGPLWLEIRIPGRG LKQQCSCFHKVRK+RER+SEILQGLFLIVIHGCQVDDDEFLVDVGCFPREET VLIVPIELPREPWSIMRLPD QEMESEPVQVELPKGEFAPLPPYPVDLDEEIERFLENYDQPTEGENRE DTER          + T +Q          + + +PD           +K+KK G GRPKKSQMKPPPNEQ+NPETIQNLTRQAL GIAKQIDDDSENHPTFKSMM RRLQ IMRLLKQDWQIPK   +   RL+ +
Sbjct:   89 AVSPGKNRWAMDNEPEACNEVLAVLPKKNLCNMVSEPEAAKATSSTRNVDGKAEIPFGRREFGRSIREETIEKCAALVKYRDQWESEVVEKLIDIDKEEIEEAEIDKILGILATLEMDKDYYYLIKNLMRKIGASPRY--------------------------------------ANETGEAVVFT----VLPGKMLLRFEINPK--ETVG-----ELVKL----------------LKEANAIPQRLGFELMQTPRDGEAIKGIMDWMLELRIPVNTLRDIMPLVLPLLKVTSSFQAQAKLLCIKIGHAIRHEASRQGPLWLEIRIPGRGLLKQQCSCFHKVRKVRERISEILQGLFLIVIHGCQVDDDEFLVDVGCFPREETLVLIVPIELPREPWSIMRLPDAQEMESEPVQVELPKGEFAPLPPYPVDLDEEIERFLENYDQPTEGENREADTERT---------KQTWVQAR------NENWTHQPDQEKS----NQRLKLKKMGGGRPKKSQMKPPPNEQINPETIQNLTRQALTGIAKQIDDDSENHPTFKSMMWRRLQPIMRLLKQDWQIPKDPGVNVKRLTVE 597
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BLAST of SMED30004099 vs. Planmine SMEST
Match: SMESG000029093.1 (SMESG000029093.1)

HSP 1 Score: 400.979 bits (1029), Expect = 5.874e-128
Identity = 203/277 (73.29%), Postives = 216/277 (77.98%), Query Frame = 2 
Query: 1094 MEIEQLNQANAIPQRLGFELMQTPRDGEAIKGIMDWMLELRIPVNTLRDIMPLVLPLLKVTSPFQAQAKLLCIKNGHAIRHETSRQGPLWLEIRIPGRGKLKQQCSCFHKVRKLRERLSEILQGLFLIVIHGCQVDDDEFLVDVGCFPREETQVLIVPIELPREPWSIMRLPDCQEMESEPVQVELPKGEFAPLPPYPVDLDEEIERFLENYDQPTEGENREVDTERVFGQQIIEDERDTIIQLDPVETDVGASTSREPDPPARPGIIQSEIKVKKD 1924
            MEIEQLN ANAIPQRLGFELMQT RDGEAIKGIMDWMLELRIPVNTLRDIMPLVLPLLK+TS FQAQ KLLCIKNGHAIRHETS  G LWLEIRIPGRG+LKQQCSCFHKVRKLRER+SEIL+ LFLIVIHGCQVDDDEFLVD+GCFPREET VLIVP E PREPWSIMRLPD +EME E                                     G NRE DT RVF +Q+IEDERDTIIQLDP ET++GASTSREPDPPARP + Q    +K+D
Sbjct:    1 MEIEQLNLANAIPQRLGFELMQTIRDGEAIKGIMDWMLELRIPVNTLRDIMPLVLPLLKMTSLFQAQPKLLCIKNGHAIRHETSGLGLLWLEIRIPGRGQLKQQCSCFHKVRKLRERISEILEELFLIVIHGCQVDDDEFLVDLGCFPREETLVLIVPFEFPREPWSIMRLPDAREMEKE-------------------------------------GANREADTGRVFEEQVIEDERDTIIQLDPEETNMGASTSREPDPPARPRLQQILNLLKQD 240
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BLAST of SMED30004099 vs. Planmine SMEST
Match: SMESG000058681.1 (SMESG000058681.1)

HSP 1 Score: 332.028 bits (850), Expect = 2.865e-102
Identity = 202/287 (70.38%), Postives = 221/287 (77.00%), Query Frame = 2 
Query:  392 LAVSPDKNRLAMVDEPEAC--NEVLAVLPEK------NRCNMVSGPEAVKATSSTRDVDGKAEIPVGRRELGKSPRKEIIEKCAALVKYRDQWESXXXXXXXXXXXXXXXXXXXXXXXGIIATLERDEDYYYLIKNLMRKIGASPRYANETSEAVVITVLPGKMLHRFEIDPKETVGELVKLLKEARPGGNYVLYKYGAEMLEGDTLLSYGILPNYNNTVDVVYPTEKVKVIDTDNVFMITEMEIEQLNQANAIPQRLGFELMQTPRDGEAIKGIMDWMLELRIPVN 1228
            L +  ++NRLA +   +    NEV  +  +K      + CN V      K  +S        E       L  SP     +KCAALVKYRDQ E EVVEKLKEIDK+EIEEAEIDKILGIIATLERDEDYYYLI+NLMRKI ASPRYAN+TSEAVV TVLPG MLHR +I+P ETVGELVK LKEARPG NYVL+KYG EML+GDTLLSYGILPNYNNTVDVVYP+EKVKVIDTDNVF ITEMEIEQLN ANAIPQRLGFELMQT RDGEAIKGIMDWMLELRIPV+
Sbjct:   89 LEIQEERNRLARLTTNDNIDDNEVYMLSSQKKHQGADHECNTVDPENDFKVRTS--------EFETSSNGLAVSP-----DKCAALVKYRDQLEGEVVEKLKEIDKKEIEEAEIDKILGIIATLERDEDYYYLIRNLMRKIEASPRYANKTSEAVVFTVLPGNMLHRLKINPNETVGELVKRLKEARPGWNYVLFKYGVEMLDGDTLLSYGILPNYNNTVDVVYPSEKVKVIDTDNVFAITEMEIEQLNLANAIPQRLGFELMQTSRDGEAIKGIMDWMLELRIPVD 362

HSP 2 Score: 276.174 bits (705), Expect = 1.697e-81
Identity = 146/215 (67.91%), Postives = 149/215 (69.30%), Query Frame = 2 
Query: 1496 VDDDEFLVDVGCFPREETQVLIVPIELPREPWSIMRLPDCQEMESEPVQVELPKGEFAPLPPYPVDLDEEIERFLENYDQPTEGE-NREVDTERVFGQQIIEDERDTIIQLDPVETDVGASTSREPDPPARPGIIQSEIKVKKDGRGRPKKSQMKPPPNEQLNPETIQNLTRQALAGIAKQIDDDSENHPTFKSMM*RRLQQIMRLLKQDWQIPK 2137
            VD DEFLVDVGCFPREET VLIVPI+LPREPWSIMRL D QEMESEPVQVELPK EFAPL PYPVDLDEEIERFLENY+QPTE E NRE DTE                                                  +G G PKKSQMKPPP EQLNPETIQNLTRQAL GIAKQIDDDSENHP FKSMM RR QQIMRLLKQDWQIPK
Sbjct:  361 VDGDEFLVDVGCFPREETLVLIVPIKLPREPWSIMRLSDAQEMESEPVQVELPKSEFAPLSPYPVDLDEEIERFLENYNQPTEEEANREADTE--------------------------------------------------NGTGNPKKSQMKPPPIEQLNPETIQNLTRQALTGIAKQIDDDSENHPAFKSMMWRRFQQIMRLLKQDWQIPK 525

HSP 3 Score: 140.198 bits (352), Expect = 4.531e-34
Identity = 82/91 (90.11%), Postives = 84/91 (92.31%), Query Frame = 2 
Query:    8 RPKTTTFAVNSEQRLTYXXXXXXXXXXXRNRLARLTTNDNIDENEVYMLSSQKKHQGKDHESNSVD*ENDSKVRTSEFVTSSIGLAVSPSK 280
            RPKTTTFAVNSEQRLTYLQQLELEIQEERNRLARLTTNDNID+NEVYMLSSQKKHQG DHE N+VD END KVRTSEF TSS GLAVSP K
Sbjct:   67 RPKTTTFAVNSEQRLTYLQQLELEIQEERNRLARLTTNDNIDDNEVYMLSSQKKHQGADHECNTVDPENDFKVRTSEFETSSNGLAVSPDK 157
[Close]
BLAST of SMED30004099 vs. Planmine SMEST
Match: SMESG000058681.1 (SMESG000058681.1)

HSP 1 Score: 332.028 bits (850), Expect = 5.389e-102
Identity = 202/287 (70.38%), Postives = 221/287 (77.00%), Query Frame = 2 
Query:  392 LAVSPDKNRLAMVDEPEAC--NEVLAVLPEK------NRCNMVSGPEAVKATSSTRDVDGKAEIPVGRRELGKSPRKEIIEKCAALVKYRDQWESXXXXXXXXXXXXXXXXXXXXXXXGIIATLERDEDYYYLIKNLMRKIGASPRYANETSEAVVITVLPGKMLHRFEIDPKETVGELVKLLKEARPGGNYVLYKYGAEMLEGDTLLSYGILPNYNNTVDVVYPTEKVKVIDTDNVFMITEMEIEQLNQANAIPQRLGFELMQTPRDGEAIKGIMDWMLELRIPVN 1228
            L +  ++NRLA +   +    NEV  +  +K      + CN V      K  +S        E       L  SP     +KCAALVKYRDQ E EVVEKLKEIDK+EIEEAEIDKILGIIATLERDEDYYYLI+NLMRKI ASPRYAN+TSEAVV TVLPG MLHR +I+P ETVGELVK LKEARPG NYVL+KYG EML+GDTLLSYGILPNYNNTVDVVYP+EKVKVIDTDNVF ITEMEIEQLN ANAIPQRLGFELMQT RDGEAIKGIMDWMLELRIPV+
Sbjct:   62 LEIQEERNRLARLTTNDNIDDNEVYMLSSQKKHQGADHECNTVDPENDFKVRTS--------EFETSSNGLAVSP-----DKCAALVKYRDQLEGEVVEKLKEIDKKEIEEAEIDKILGIIATLERDEDYYYLIRNLMRKIEASPRYANKTSEAVVFTVLPGNMLHRLKINPNETVGELVKRLKEARPGWNYVLFKYGVEMLDGDTLLSYGILPNYNNTVDVVYPSEKVKVIDTDNVFAITEMEIEQLNLANAIPQRLGFELMQTSRDGEAIKGIMDWMLELRIPVD 335

HSP 2 Score: 276.559 bits (706), Expect = 2.731e-81
Identity = 146/215 (67.91%), Postives = 149/215 (69.30%), Query Frame = 2 
Query: 1496 VDDDEFLVDVGCFPREETQVLIVPIELPREPWSIMRLPDCQEMESEPVQVELPKGEFAPLPPYPVDLDEEIERFLENYDQPTEGE-NREVDTERVFGQQIIEDERDTIIQLDPVETDVGASTSREPDPPARPGIIQSEIKVKKDGRGRPKKSQMKPPPNEQLNPETIQNLTRQALAGIAKQIDDDSENHPTFKSMM*RRLQQIMRLLKQDWQIPK 2137
            VD DEFLVDVGCFPREET VLIVPI+LPREPWSIMRL D QEMESEPVQVELPK EFAPL PYPVDLDEEIERFLENY+QPTE E NRE DTE                                                  +G G PKKSQMKPPP EQLNPETIQNLTRQAL GIAKQIDDDSENHP FKSMM RR QQIMRLLKQDWQIPK
Sbjct:  334 VDGDEFLVDVGCFPREETLVLIVPIKLPREPWSIMRLSDAQEMESEPVQVELPKSEFAPLSPYPVDLDEEIERFLENYNQPTEEEANREADTE--------------------------------------------------NGTGNPKKSQMKPPPIEQLNPETIQNLTRQALTGIAKQIDDDSENHPAFKSMMWRRFQQIMRLLKQDWQIPK 498

HSP 3 Score: 140.198 bits (352), Expect = 4.727e-34
Identity = 82/91 (90.11%), Postives = 84/91 (92.31%), Query Frame = 2 
Query:    8 RPKTTTFAVNSEQRLTYXXXXXXXXXXXRNRLARLTTNDNIDENEVYMLSSQKKHQGKDHESNSVD*ENDSKVRTSEFVTSSIGLAVSPSK 280
            RPKTTTFAVNSEQRLTYLQQLELEIQEERNRLARLTTNDNID+NEVYMLSSQKKHQG DHE N+VD END KVRTSEF TSS GLAVSP K
Sbjct:   40 RPKTTTFAVNSEQRLTYLQQLELEIQEERNRLARLTTNDNIDDNEVYMLSSQKKHQGADHECNTVDPENDFKVRTSEFETSSNGLAVSPDK 130
The following BLAST results are available for this feature:
BLAST of SMED30004099 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30004099 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000035043.10.000e+070.49SMESG000035043.1[more]
SMESG000029093.15.874e-12873.29SMESG000029093.1[more]
SMESG000058681.12.865e-10270.38SMESG000058681.1[more]
SMESG000058681.15.389e-10270.38SMESG000058681.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004099 ID=SMED30004099|Name=SMED30004099|organism=Schmidtea mediterranea sexual|type=transcript|length=2243bp
TAATAATCGGCCAAAAACAACTACGTTTGCAGTTAATTCGGAACAGAGAT
TAACGTATCTACAACAACTCGAATTAGAAATCCAAGAGGAAAGAAATAGG
TTAGCTCGGCTAACAACAAATGATAATATTGATGAAAATGAGGTTTATAT
GCTTTCGAGCCAGAAAAAGCATCAAGGAAAGGACCATGAGTCTAATTCCG
TGGATTAGGAAAATGATTCCAAGGTTAGAACAAGTGAGTTCGTAACCAGC
AGTATTGGTTTAGCGGTTTCACCAAGTAAAAATCGATTGGCTACGGACAA
TGAGCCAGAAGCCAGAATAAAGGGTTTAGCGGTTTCACCAGGTAAAAATC
GATTGGCTATGGACAATGAACCAGAGGCCAGAACAATTGGTTTAGCGGTT
TCACCAGATAAAAATCGATTGGCTATGGTCGATGAGCCAGAAGCCTGCAA
TGAGGTTTTAGCGGTTTTGCCCGAGAAAAATCGATGTAATATGGTCAGCG
GACCAGAAGCAGTAAAAGCCACGTCATCCACAAGAGATGTTGATGGAAAA
GCTGAAATACCGGTCGGTAGACGAGAACTTGGGAAATCTCCCAGAAAGGA
AATTATAGAAAAATGTGCAGCATTAGTAAAATATCGCGATCAATGGGAAA
GCGAGGTAGTAGAGAAATTAAAAGAGATTGATAAAGAGGAAATTGAGGAA
GCTGAAATTGATAAAATTCTCGGAATAATAGCCACCCTAGAAAGGGATGA
AGACTATTATTATCTGATAAAAAACCTAATGAGGAAAATCGGAGCAAGCC
CGAGATATGCCAATGAAACAAGTGAGGCAGTAGTGATTACAGTATTACCT
GGGAAAATGCTGCATAGGTTTGAAATAGACCCCAAGGAAACTGTGGGAGA
ACTCGTGAAACTATTAAAAGAAGCGAGGCCCGGTGGTAATTATGTGTTGT
ATAAATATGGAGCTGAAATGCTAGAGGGAGACACGCTATTGAGTTATGGA
ATATTGCCAAACTACAATAATACGGTAGACGTGGTTTACCCGACAGAAAA
AGTAAAAGTGATAGACACAGATAATGTGTTTATGATAACTGAAATGGAAA
TTGAACAGTTAAATCAGGCTAACGCAATTCCGCAAAGGTTGGGATTCGAG
CTAATGCAAACCCCTAGAGATGGGGAAGCCATTAAAGGAATAATGGATTG
GATGTTAGAATTACGCATCCCAGTAAATACTTTGAGAGATATAATGCCAT
TGGTGCTGCCTTTATTAAAAGTGACTTCACCATTTCAGGCACAAGCAAAG
CTGTTATGTATCAAAAATGGACATGCTATTCGCCATGAAACCAGCAGACA
AGGTCCATTATGGTTAGAAATCAGAATACCCGGGAGAGGGAAATTGAAGC
AACAATGTTCCTGTTTTCACAAGGTGAGAAAACTCAGGGAGAGACTTTCG
GAGATATTGCAAGGATTATTTCTAATAGTGATACACGGATGCCAGGTGGA
CGATGACGAATTCCTGGTAGACGTGGGATGTTTCCCAAGAGAGGAAACCC
AAGTGTTAATAGTACCAATTGAACTTCCAAGGGAGCCATGGTCAATAATG
AGATTGCCCGACTGCCAGGAAATGGAAAGTGAACCAGTACAGGTGGAATT
GCCAAAGGGTGAATTTGCACCACTACCTCCATATCCGGTGGATTTGGATG
AGGAAATTGAGAGATTTCTTGAAAACTATGATCAACCCACAGAGGGAGAA
AATAGAGAAGTTGATACCGAGAGAGTATTCGGACAGCAGATAATAGAGGA
CGAGAGAGATACAATTATCCAGCTCGATCCAGTAGAAACAGATGTGGGTG
CAAGCACGTCACGAGAACCAGACCCACCAGCCAGACCAGGAATAATCCAA
TCAGAGATTAAAGTTAAAAAAGATGGGAGGGGTAGACCAAAGAAAAGTCA
AATGAAACCACCCCCAAATGAACAATTAAATCCAGAGACAATACAGAATT
TAACTCGTCAAGCGTTGGCGGGGATAGCAAAACAAATTGATGATGATAGT
GAAAATCATCCAACATTTAAATCAATGATGTGAAGAAGATTGCAACAAAT
CATGAGGCTTTTAAAACAAGACTGGCAAATCCCAAAAGTGAAACAGTTAA
GAGCATTAAGACTCTCGACAGATAATTGTACATGCTCAATTACGAATAAG
GGTAAAATCCCGCTATGGATAATAATTTCAATAACGGGGGGAA
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protein sequence of SMED30004099-orf-1

>SMED30004099-orf-1 ID=SMED30004099-orf-1|Name=SMED30004099-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=575bp
MDNEPEARTIGLAVSPDKNRLAMVDEPEACNEVLAVLPEKNRCNMVSGPE
AVKATSSTRDVDGKAEIPVGRRELGKSPRKEIIEKCAALVKYRDQWESEV
VEKLKEIDKEEIEEAEIDKILGIIATLERDEDYYYLIKNLMRKIGASPRY
ANETSEAVVITVLPGKMLHRFEIDPKETVGELVKLLKEARPGGNYVLYKY
GAEMLEGDTLLSYGILPNYNNTVDVVYPTEKVKVIDTDNVFMITEMEIEQ
LNQANAIPQRLGFELMQTPRDGEAIKGIMDWMLELRIPVNTLRDIMPLVL
PLLKVTSPFQAQAKLLCIKNGHAIRHETSRQGPLWLEIRIPGRGKLKQQC
SCFHKVRKLRERLSEILQGLFLIVIHGCQVDDDEFLVDVGCFPREETQVL
IVPIELPREPWSIMRLPDCQEMESEPVQVELPKGEFAPLPPYPVDLDEEI
ERFLENYDQPTEGENREVDTERVFGQQIIEDERDTIIQLDPVETDVGAST
SREPDPPARPGIIQSEIKVKKDGRGRPKKSQMKPPPNEQLNPETIQNLTR
QALAGIAKQIDDDSENHPTFKSMM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell