Abhydrolase_3 domain-containing protein

Overview
NameAbhydrolase_3 domain-containing protein
Smed IDSMED30004072
Length (bp)1262
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Abhydrolase_3 domain-containing protein (SMED30004072) t-SNE clustered cells

Violin plots show distribution of expression levels for Abhydrolase_3 domain-containing protein (SMED30004072) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Abhydrolase_3 domain-containing protein (SMED30004072) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Abhydrolase_3 domain-containing protein (SMED30004072) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004072

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30004072SMESG000069978.1 Contig4628newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30004072SMESG000069978.1 Contig4628uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30004072SMESG000003820.1 Contig4628newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30004072SMESG000003820.1 Contig4628uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
parenchymal cellSMED30004072SMESG000069978.1 dd_Smed_v4_8282_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
central nervous systemSMED30004072SMESG000069978.1 SMU15016987SMUPMID:27240733
Stubenhaus et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30004072SMESG000069978.1 SMU15016987SMUPMID:27240733
Stubenhaus et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
pigment cellSMED30004072SMESG000069978.1 SMU15016987SMUPMID:27240733
Stubenhaus et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Human
Match: AFMID (arylformamidase [Source:HGNC Symbol;Acc:HGNC:20910])

HSP 1 Score: 90.5077 bits (223), Expect = 1.084e-19
Identity = 67/229 (29.26%), Postives = 102/229 (44.54%), Query Frame = 3 
Query:  246 FFPSKH-ESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHP-DQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFV-KEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            +FP +  E+    ++ HGG W +     S+F+     +     V + +   P  ++  M+ QV  ++  +   +P ++ +Y+ GHS+GAHL    LL DW          +   LRG  L+ G FDL  I  +  N   QLT ++A R SP   V           +L++V + D  EF RQS   Y  + +   K  F  L     VDHF IV  L      LT  I+
Sbjct:   79 YFPDESSEALPFFLFFHGGYWQSGSKDESAFMVHPLTAQGVAVVIVAYGIAPKGTLDHMVDQVTRSVAFVQKRYPSNKGIYLCGHSAGAHLAAMMLLADWT------KHGVTPNLRGFFLVSGVFDLEPIVYTSQNVALQLTLEDAQRNSPQLKVAQAQPVDPTCRVLVVVGQFDSPEFHRQSWEFYQTLCQGEWKASFEEL---HDVDHFEIVENLTQKDNVLTQIIL 298
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Human
Match: AFMID (arylformamidase [Source:HGNC Symbol;Acc:HGNC:20910])

HSP 1 Score: 88.9669 bits (219), Expect = 4.024e-19
Identity = 65/231 (28.14%), Postives = 103/231 (44.59%), Query Frame = 3 
Query:  246 FFPSKH-ESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHP-DQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLY--YEMKRFVKEEF-LHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            +FP +  E+    ++ HGG W +     S+F+     +     V + +   P  ++  M+ QV  ++  +   +P ++ +Y+ GHS+GAHL    LL DW          +   LRG  L+ G FDL  I  +  N   QLT ++A R SP   V           +L++V + D  EF RQS   Y    ++   + E+      +  VDHF IV  L      LT  I+
Sbjct:   79 YFPDESSEALPFFLFFHGGYWQSGSKDESAFMVHPLTAQGVAVVIVAYGIAPKGTLDHMVDQVTRSVAFVQKRYPSNKGIYLCGHSAGAHLAAMMLLADWT------KHGVTPNLRGFFLVSGVFDLEPIVYTSQNVALQLTLEDAQRNSPQLKVAQAQPVDPTCRVLVVVGQFDSPEFHRQSWEFYQVLPVQTLCQGEWKASFEELHDVDHFEIVENLTQKDNVLTQIIL 303
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Celegans
Match: afmd-1 (ArylForMamiDase [Source:UniProtKB/TrEMBL;Acc:Q18974])

HSP 1 Score: 55.8398 bits (133), Expect = 1.581e-8
Identity = 48/211 (22.75%), Postives = 100/211 (47.39%), Query Frame = 3 
Query:  267 SNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFD-PCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGN 893
            S+ L+I++HGG W           A   ++N Y F ++G+       +++E +  V   +  I   +P+  NV + GHS+GAHL ++++           ++    R+RG++L  G + L E+  +   +   LT+D+A   S    +  +   + D ++++ ++E    +   Q+++   +  +   EEF +        H+TI++ L N
Sbjct:   66 SDKLLIFIHGGYWAAGTRKDCLTPARCALNNEYAFASVGYGLSTEGRTLTETVEDVVNGVDFILKLYPNVSNVLVGGHSAGAHLAMNAV-----------ARLRNPRIRGLLLFSGCYFLEELIGTEIGTDINLTTDQAKLNSC--DLSKLDGLKLDSLVILGLQEAP--KLIEQNRDFVAQQGQSRIEEFPN------SGHYTIMTNLLN 255
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Fly
Match: KFase (gene:FBgn0031821 transcript:FBtr0079265)

HSP 1 Score: 47.7506 bits (112), Expect = 9.163e-6
Identity = 53/211 (25.12%), Postives = 93/211 (44.08%), Query Frame = 3 
Query:  276 LVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPDQNV---YIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYK----SPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITV-DGVDHFTIVSQ 884
            L ++VHGG W  +D+S S  +    V   Y    + ++ CP +++ +++ Q    +  IF       V      GHS+GAH     LL   L   +  +    + +  ++ + G +DLRE+      +P N L  L       VSP+ +        +   I ++  E D   F  QS++ Y ++ R  K+ +    T+  G DHF I+ +
Sbjct:   81 LFVFVHGGYWQEMDMSMSCSIVGPLVRRGYRVAVMDYNLCPQVTLEQLMTQFTHFLNWIFDYTEMTKVSSLTFAGHSAGAH-----LLAQILMRPNVITAQRSKMVWALIFLCGVYDLRELSNLESVNPKNIL-GLNERNIESVSPMLWEYTDVTVWNSTKIYVVAAEHDSTTFIEQSRH-YADVLR--KKGYKASFTLFKGYDHFDIIEE 282
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Fly
Match: KFase (gene:FBgn0031821 transcript:FBtr0339785)

HSP 1 Score: 47.7506 bits (112), Expect = 9.163e-6
Identity = 53/211 (25.12%), Postives = 93/211 (44.08%), Query Frame = 3 
Query:  276 LVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPDQNV---YIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYK----SPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITV-DGVDHFTIVSQ 884
            L ++VHGG W  +D+S S  +    V   Y    + ++ CP +++ +++ Q    +  IF       V      GHS+GAH     LL   L   +  +    + +  ++ + G +DLRE+      +P N L  L       VSP+ +        +   I ++  E D   F  QS++ Y ++ R  K+ +    T+  G DHF I+ +
Sbjct:   81 LFVFVHGGYWQEMDMSMSCSIVGPLVRRGYRVAVMDYNLCPQVTLEQLMTQFTHFLNWIFDYTEMTKVSSLTFAGHSAGAH-----LLAQILMRPNVITAQRSKMVWALIFLCGVYDLRELSNLESVNPKNIL-GLNERNIESVSPMLWEYTDVTVWNSTKIYVVAAEHDSTTFIEQSRH-YADVLR--KKGYKASFTLFKGYDHFDIIEE 282
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Zebrafish
Match: cant1b (calcium activated nucleotidase 1b [Source:ZFIN;Acc:ZDB-GENE-040801-114])

HSP 1 Score: 86.2705 bits (212), Expect = 1.345e-18
Identity = 67/229 (29.26%), Postives = 111/229 (48.47%), Query Frame = 3 
Query:  246 FFPSKHESN-NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRF-VKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            + PS    +  LVIY HGG W  L    S FLA   V      VA+G+   P   +  M+ QV+ ++ ++   +     +Y+ GHS+GAHL    L  DW    + +  S   +++G  L+ G +DL+ I  +  N   ++T + A R SP +++  +        I + V + D  EF++QS+  Y  ++   +K  F     V   DHF+I+ QL +  Y LT  ++
Sbjct:   44 YLPSSSSPDVPLVIYFHGGYWQFLSKDESGFLAVPLVQKGAVVVAVGYSIAPKGDMDLMVSQVRRSVVSVIQQYSHISGLYLCGHSAGAHLAAMVLSTDW----TQYDVS--PQIKGAFLVSGIYDLQPILSTYVNEPLKMTEEVALRNSPSRFLSQLKVSSASCDITVAVAQNDSPEFRKQSEEYYKALESAGLKVSFED---VSETDHFSIIEQLVDENYLLTKLLL 263
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Zebrafish
Match: afmid (arylformamidase [Source:NCBI gene;Acc:550372])

HSP 1 Score: 86.2705 bits (212), Expect = 1.345e-18
Identity = 67/229 (29.26%), Postives = 111/229 (48.47%), Query Frame = 3 
Query:  246 FFPSKHESN-NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRF-VKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            + PS    +  LVIY HGG W  L    S FLA   V      VA+G+   P   +  M+ QV+ ++ ++   +     +Y+ GHS+GAHL    L  DW    + +  S   +++G  L+ G +DL+ I  +  N   ++T + A R SP +++  +        I + V + D  EF++QS+  Y  ++   +K  F     V   DHF+I+ QL +  Y LT  ++
Sbjct:   44 YLPSSSSPDVPLVIYFHGGYWQFLSKDESGFLAVPLVQKGAVVVAVGYSIAPKGDMDLMVSQVRRSVVSVIQQYSHISGLYLCGHSAGAHLAAMVLSTDW----TQYDVS--PQIKGAFLVSGIYDLQPILSTYVNEPLKMTEEVALRNSPSRFLSQLKVSSASCDITVAVAQNDSPEFRKQSEEYYKALESAGLKVSFED---VSETDHFSIIEQLVDENYLLTKLLL 263
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Zebrafish
Match: afmid (arylformamidase [Source:NCBI gene;Acc:550372])

HSP 1 Score: 86.2705 bits (212), Expect = 1.812e-18
Identity = 67/229 (29.26%), Postives = 111/229 (48.47%), Query Frame = 3 
Query:  246 FFPSKHESN-NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRF-VKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            + PS    +  LVIY HGG W  L    S FLA   V      VA+G+   P   +  M+ QV+ ++ ++   +     +Y+ GHS+GAHL    L  DW    + +  S   +++G  L+ G +DL+ I  +  N   ++T + A R SP +++  +        I + V + D  EF++QS+  Y  ++   +K  F     V   DHF+I+ QL +  Y LT  ++
Sbjct:   68 YLPSSSSPDVPLVIYFHGGYWQFLSKDESGFLAVPLVQKGAVVVAVGYSIAPKGDMDLMVSQVRRSVVSVIQQYSHISGLYLCGHSAGAHLAAMVLSTDW----TQYDVS--PQIKGAFLVSGIYDLQPILSTYVNEPLKMTEEVALRNSPSRFLSQLKVSSASCDITVAVAQNDSPEFRKQSEEYYKALESAGLKVSFED---VSETDHFSIIEQLVDENYLLTKLLL 287
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Zebrafish
Match: afmid (arylformamidase [Source:NCBI gene;Acc:550372])

HSP 1 Score: 84.7297 bits (208), Expect = 3.094e-18
Identity = 65/218 (29.82%), Postives = 107/218 (49.08%), Query Frame = 3 
Query:  276 LVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRF-VKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            LVIY HGG W  L    S FLA   V      VA+G+   P   +  M+ QV+ ++ ++   +     +Y+ GHS+GAHL    L  DW    + +  S   +++G  L+ G +DL+ I  +  N   ++T + A R SP +++  +        I + V + D  EF++QS+  Y  ++   +K  F     V   DHF+I+ QL +  Y LT  ++
Sbjct:   44 LVIYFHGGYWQFLSKDESGFLAVPLVQKGAVVVAVGYSIAPKGDMDLMVSQVRRSVVSVIQQYSHISGLYLCGHSAGAHLAAMVLSTDW----TQYDVS--PQIKGAFLVSGIYDLQPILSTYVNEPLKMTEEVALRNSPSRFLSQLKVSSASCDITVAVAQNDSPEFRKQSEEYYKALESAGLKVSFED---VSETDHFSIIEQLVDENYLLTKLLL 252
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Zebrafish
Match: afmid (arylformamidase [Source:NCBI gene;Acc:550372])

HSP 1 Score: 80.4925 bits (197), Expect = 1.565e-16
Identity = 64/217 (29.49%), Postives = 105/217 (48.39%), Query Frame = 3 
Query:  246 FFPSKHESN-NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRF-VKEEFLHLITVDGVDHFTIVSQL 887
            + PS    +  LVIY HGG W  L    S FLA   V      VA+G+   P   +  M+ QV+ ++ ++   +     +Y+ GHS+GAHL    L  DW    + +  S   +++G  L+ G +DL+ I  +  N   ++T + A R SP +++  +        I + V + D  EF++QS+  Y  ++   +K  F     V   DHF+I+ QL
Sbjct:   68 YLPSSSSPDVPLVIYFHGGYWQFLSKDESGFLAVPLVQKGAVVVAVGYSIAPKGDMDLMVSQVRRSVVSVIQQYSHISGLYLCGHSAGAHLAAMVLSTDW----TQYDVS--PQIKGAFLVSGIYDLQPILSTYVNEPLKMTEEVALRNSPSRFLSQLKVSSASCDITVAVAQNDSPEFRKQSEEYYKALESAGLKVSFED---VSETDHFSIIEQL 275
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Xenopus
Match: afmid (arylformamidase [Source:NCBI gene;Acc:100127853])

HSP 1 Score: 95.1301 bits (235), Expect = 2.029e-21
Identity = 61/222 (27.48%), Postives = 111/222 (50.00%), Query Frame = 3 
Query:  276 LVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIER-LRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYLR 935
            L++Y+HGG W  L    S F+    V +    + + +D  P   +  ++ QV+ +I      +P    +Y+ GHS+GAHL   +L   W       S+ M++  ++G VL+ G +DL  I  +  N   +++  +A R SP++ +  + ++     I I+V E D  EF RQS+  +  ++       +    +DG DHF ++ QL +  Y LT  I++ ++
Sbjct:   80 LLVYIHGGYWQFLSKEESGFMVPPLVLHGIGVMVMDYDIAPQGHMDLIVSQVRQSIAVTIQRYPQITGIYLCGHSAGAHLVAMTLCTKW-------SEYMVKPDIKGAVLVSGVYDLLPIIHTYVNDALKMSQTDAERNSPMRCLTQMESHIRTCQIAIVVAEHDSPEFHRQSQEYFQSLQAVGFS--VTFTQIDGTDHFDVIEQLHDEKYVLTQMILEMIK 292
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Mouse
Match: Afmid (arylformamidase [Source:MGI Symbol;Acc:MGI:2448704])

HSP 1 Score: 92.4337 bits (228), Expect = 1.749e-20
Identity = 67/262 (25.57%), Postives = 122/262 (46.56%), Query Frame = 3 
Query:  153 IANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSK-HESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHP-DQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISN--YRHDLMILILVEEIDYREFQRQSKNLYYEMKRFV-KEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            I +++    +  +       Y +   E    +FP +  ++  L +++HGG W +     S+F+ +   +     V + +D  P  ++ +M+ QV  ++  +   +P ++ +Y+ GHS+GAHL    LL  W          +   L+G +L+ G +DL  +  +  N   ++T ++A R SP ++++ +          +L+LV + D  EF RQSK  Y  + R   K  F  L    GVDHF I+  L      LT  I+
Sbjct:   46 IGSQATQKARATRRNQLDVPYGDGEGEKLDIYFPDEDSKAFPLFLFLHGGYWQSGSKDDSAFMVNPLTAQGIVVVIVAYDIAPKGTLDQMVDQVTRSVVFLQRRYPSNEGIYLCGHSAGAHLAAMVLLARWT------KHGVTPNLQGFLLVSGIYDLEPLIATSQNDPLRMTLEDAQRNSPQRHLDVVPAQPVAPACPVLVLVGQHDSPEFHRQSKEFYETLLRVGWKASFQQL---RGVDHFDIIENLTREDDVLTQIIL 298
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Mouse
Match: Afmid (arylformamidase [Source:MGI Symbol;Acc:MGI:2448704])

HSP 1 Score: 89.7373 bits (221), Expect = 2.528e-19
Identity = 64/250 (25.60%), Postives = 118/250 (47.20%), Query Frame = 3 
Query:  153 IANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSK-HESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHP-DQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISN--YRHDLMILILVEEIDYREFQRQSKNLYYEMKRFV-KEEFLHLITVDGVDHFTIVSQL 887
            I +++    +  +       Y +   E    +FP +  ++  L +++HGG W +     S+F+ +   +     V + +D  P  ++ +M+ QV  ++  +   +P ++ +Y+ GHS+GAHL    LL  W          +   L+G +L+ G +DL  +  +  N   ++T ++A R SP ++++ +          +L+LV + D  EF RQSK  Y  + R   K  F  L    GVDHF I+  L
Sbjct:   38 IGSQATQKARATRRNQLDVPYGDGEGEKLDIYFPDEDSKAFPLFLFLHGGYWQSGSKDDSAFMVNPLTAQGIVVVIVAYDIAPKGTLDQMVDQVTRSVVFLQRRYPSNEGIYLCGHSAGAHLAAMVLLARWT------KHGVTPNLQGFLLVSGIYDLEPLIATSQNDPLRMTLEDAQRNSPQRHLDVVPAQPVAPACPVLVLVGQHDSPEFHRQSKEFYETLLRVGWKASFQQL---RGVDHFDIIENL 278
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8K4H1|KFA_MOUSE (Kynurenine formamidase OS=Mus musculus OX=10090 GN=Afmid PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.223e-19
Identity = 67/262 (25.57%), Postives = 122/262 (46.56%), Query Frame = 3 
Query:  153 IANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSK-HESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHP-DQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISN--YRHDLMILILVEEIDYREFQRQSKNLYYEMKRFV-KEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            I +++    +  +       Y +   E    +FP +  ++  L +++HGG W +     S+F+ +   +     V + +D  P  ++ +M+ QV  ++  +   +P ++ +Y+ GHS+GAHL    LL  W          +   L+G +L+ G +DL  +  +  N   ++T ++A R SP ++++ +          +L+LV + D  EF RQSK  Y  + R   K  F  L    GVDHF I+  L      LT  I+
Sbjct:   46 IGSQATQKARATRRNQLDVPYGDGEGEKLDIYFPDEDSKAFPLFLFLHGGYWQSGSKDDSAFMVNPLTAQGIVVVIVAYDIAPKGTLDQMVDQVTRSVVFLQRRYPSNEGIYLCGHSAGAHLAAMVLLARWT------KHGVTPNLQGFLLVSGIYDLEPLIATSQNDPLRMTLEDAQRNSPQRHLDVVPAQPVAPACPVLVLVGQHDSPEFHRQSKEFYETLLRVGWKASFQQL---RGVDHFDIIENLTREDDVLTQIIL 298
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q63HM1|KFA_HUMAN (Kynurenine formamidase OS=Homo sapiens OX=9606 GN=AFMID PE=1 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 5.205e-19
Identity = 67/229 (29.26%), Postives = 102/229 (44.54%), Query Frame = 3 
Query:  246 FFPSKH-ESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHP-DQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFV-KEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            +FP +  E+    ++ HGG W +     S+F+     +     V + +   P  ++  M+ QV  ++  +   +P ++ +Y+ GHS+GAHL    LL DW          +   LRG  L+ G FDL  I  +  N   QLT ++A R SP   V           +L++V + D  EF RQS   Y  + +   K  F  L     VDHF IV  L      LT  I+
Sbjct:   79 YFPDESSEALPFFLFFHGGYWQSGSKDESAFMVHPLTAQGVAVVIVAYGIAPKGTLDHMVDQVTRSVAFVQKRYPSNKGIYLCGHSAGAHLAAMMLLADWT------KHGVTPNLRGFFLVSGVFDLEPIVYTSQNVALQLTLEDAQRNSPQLKVAQAQPVDPTCRVLVVVGQFDSPEFHRQSWEFYQTLCQGEWKASFEEL---HDVDHFEIVENLTQKDNVLTQIIL 298
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. UniProt/SwissProt
Match: sp|B5XB27|KFA_SALSA (Kynurenine formamidase OS=Salmo salar OX=8030 GN=afmid PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.917e-18
Identity = 69/221 (31.22%), Postives = 112/221 (50.68%), Query Frame = 3 
Query:  276 LVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQ-SMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYL 932
            LVIY+HGG W  L    S F+A   V      VA+G+D  P  ++  M+ QV+ ++ ++   +     +Y+ GHS+GAHL    L  DW       SQ S+  +++G  L+ G +DL  I  +  N   ++T + A R SP Q V  +     +  I++ V + D  EF++QS++ YY+     +   + L  V   DHF I+ QL +  Y LT  ++  +
Sbjct:   79 LVIYLHGGYWQFLSKEESGFMAVPLVHKGVVVVAVGYDIAPKGNMDVMVSQVRRSVVSVIQQYSHISGLYLCGHSAGAHLAAMILSTDW-------SQYSVTPQIKGAFLVSGIYDLLPILSTYVNEPLKMTEEVALRNSPSQLVPQLKLSSSNCDIVVAVAQNDSPEFRKQSED-YYKALESTEGLKVTLEDVPNTDHFNIIEQLVDGDYHLTQLLLKMM 291
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q566U4|KFA_DANRE (Kynurenine formamidase OS=Danio rerio OX=7955 GN=afmid PE=2 SV=2)

HSP 1 Score: 86.6557 bits (213), Expect = 8.970e-18
Identity = 67/229 (29.26%), Postives = 111/229 (48.47%), Query Frame = 3 
Query:  246 FFPSKHESN-NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRF-VKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            + PS    +  LVIY HGG W  L    S FLA   V      VA+G+   P   +  M+ QV+ ++ ++   +     +Y+ GHS+GAHL    L  DW    + +  S   +++G  L+ G +DL+ I  +  N   ++T + A R SP +++  +        I + V + D  EF++QS+  Y  ++   +K  F     V   DHF+I+ QL +  Y LT  ++
Sbjct:   68 YLPSSSSPDVPLVIYFHGGYWQFLSKDESGFLAVPLVQKGAVVVAVGYSIAPKGDMDLMVSQVRRSVVSVIQQYSHISGLYLCGHSAGAHLAAMVLSTDW----TQYDVS--PKIKGAFLVSGIYDLQPILSTYVNEPLKMTEEVALRNSPSRFLSQLKVSSASCDITVAVAQNDSPEFRKQSEEYYKALESAGLKVSFED---VSETDHFSIIEQLVDENYLLTKLLL 287
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. TrEMBL
Match: A0A1S3KC92 (Kynurenine formamidase OS=Lingula unguis OX=7574 GN=LOC106180725 PE=3 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 1.730e-27
Identity = 82/271 (30.26%), Postives = 144/271 (53.14%), Query Frame = 3 
Query:  144 LEFIANKSNDMKKKLKYETFSYNYKNMSDENKYE-FFPSKHESN-NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIF---TNHPDQNVYIIGHSSGAHLTLSSLLCDWLDE---SSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYL 932
            ++ +   S   ++ L++ +  Y     S+E K + F+PS ++    + +Y+HGG W ++ LS SSF+A+ FV +      +G+D  P +++ ++I QVK A+ ++           VYI GHS+GAHL    L  DW+ E   S  F       L+G+ L+ G FDLR +  +  N   ++T +EAW  SP+ ++ +I+  +    +++LV E D  EF++Q+K  +  ++          + V GVDHF +V +L  P Y LT   ++ +
Sbjct:   31 VQVLTEGSERARQNLEHSSIVYGE---SEEQKVDVFYPSGNKQGLPIFVYIHGGYWQDMSLSVSSFMAETFVGSGAIVAVVGYDLAPKVNMGQIINQVKKALGSVMYLAKKRESSGVYICGHSAGAHLAAMMLSVDWMGEFMESPGF-------LKGVFLVSGIFDLRPLVATYVNEPLKMTDEEAWTHSPMNHLTDITCRK--CKVVVLVAEHDSPEFRKQAKEYHTALQE--AGMATQFVDVPGVDHFDVVEKLSEPQYQLTQLYLEMM 287
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. TrEMBL
Match: A0A1S3KCT5 (Kynurenine formamidase OS=Lingula unguis OX=7574 GN=LOC106180725 PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 4.159e-27
Identity = 82/264 (31.06%), Postives = 141/264 (53.41%), Query Frame = 3 
Query:  165 SNDMKKKLKYETFSYNYKNMSDENKYE-FFPSKHESN-NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIF---TNHPDQNVYIIGHSSGAHLTLSSLLCDWLDE---SSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYL 932
            S   ++ L++ +  Y     S+E K + F+PS ++    + +Y+HGG W ++ LS SSF+A+ FV +      +G+D  P +++ ++I QVK A+ ++           VYI GHS+GAHL    L  DW+ E   S  F       L+G+ L+ G FDLR +  +  N   ++T +EAW  SP+ ++ +I+  +    +++LV E D  EF++Q+K  +  ++          + V GVDHF +V +L  P Y LT   ++ +
Sbjct:   32 SERARQNLEHSSIVYGE---SEEQKVDVFYPSGNKQGLPIFVYIHGGYWQDMSLSVSSFMAETFVGSGAIVAVVGYDLAPKVNMGQIINQVKKALGSVMYLAKKRESSGVYICGHSAGAHLAAMMLSVDWMGEFMESPGF-------LKGVFLVSGIFDLRPLVATYVNEPLKMTDEEAWTHSPMNHLTDITCRK--CKVVVLVAEHDSPEFRKQAKEYHTALQE--AGMATQFVDVPGVDHFDVVEKLSEPQYQLTQLYLEMM 281
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. TrEMBL
Match: T1EP92 (Abhydrolase_3 domain-containing protein OS=Helobdella robusta OX=6412 GN=20198392 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 6.976e-26
Identity = 85/273 (31.14%), Postives = 143/273 (52.38%), Query Frame = 3 
Query:  144 LEFIANKSNDMKKKLKYETFSYNYK-NMSDENKYEFFPSK--HESNNLVIYVHGGMWINXXXXXXXX---XXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTI---FTNHPDQNVYIIGHSSGAHLTLSSLL-CDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPL---QYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFII 923
            ++ I   S++  K LK E    N K   SD+  Y+   +    + N + ++ HGG W  L  S+      +    VS N  +V++G++  P +S++EM+ Q  +AI +I   F      N+Y+ GHS+GAHL L+S   CD L   S +       ++G VL+ G FD R +Y+S  N +  +T +E+  +SPL   ++++N++ Y       I+  E D   F+R+S  LY  +++  K E +     +G+DHF +V +L  P YF+T  I+
Sbjct:   45 IDLINEASDNASKVLKIEE---NIKYGPSDKQAYDIVTNDGVTKKNPVFVFFHGGYWQALRSSTRQQYHYIGRPIVSKNATYVSVGYNLAPNVSLTEMVKQASVAISSIVKYFVALECSNIYLCGHSAGAHLALTSTTTCDDLPFISQY-------IKGFVLLSGIFDARPVYESSENKILHMTREESCEISPLVSEKHIKNLAAYLKHAAYFIVAAEQDPPLFKRESLELYRILQK-EKFERIEFSIAEGMDHFNLVERLIEPEYFVTKKIL 306
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. TrEMBL
Match: A0A0B6YHH5 (Abhydrolase_3 domain-containing protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF24002 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 8.401e-23
Identity = 66/212 (31.13%), Postives = 110/212 (51.89%), Query Frame = 3 
Query:  225 SDENKYEFFPSKHESNN---LVIYVHGGMWINXXXXXXXXXXXXXXXNNYN--FVAIGFDPCPTISISEMIVQVKLAIKTIFT---NHPDQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSM---IERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKE 827
            S+  K+  F  K+ S     +++Y+HGG W   +L   +         N     +A+G+D  P +S++ +I+Q+K A+  I           +Y+ GHS+GAHL +  L+       S FS+      E +RG VL+ G +DLR + K+  N    L+ DEAWR SPL +++ IS +     +++ V E D  EF+RQS     EM++ +++
Sbjct:   57 SERQKFNIFHKKNASTRGAPIMVYIHGGYWQTKELGRENSSFLAVPLCNAGATVIAVGYDLAPEVSMNRIILQIKKALHLIIKLAKERQSSGIYLCGHSAGAHLAIMMLM-------SAFSEDDEYDCELIRGAVLVSGVYDLRPLTKTSMNVALGLSEDEAWRFSPLNFIQEISQHSQYRHVIVAVGEYDAPEFRRQSG----EMEKILRD 257
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. TrEMBL
Match: A0A3Q0FPC1 (kynurenine formamidase OS=Alligator sinensis OX=38654 GN=AFMID PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.019e-22
Identity = 71/220 (32.27%), Postives = 109/220 (49.55%), Query Frame = 3 
Query:  276 LVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYL 932
            LV+YVHGG W  L    S F A   +S     VA+G+D  P  ++  M+ QV+ ++  I   +P    VY+ GHS+GAHL    L  DW +        +   ++G  L+ G +DL  I  +  N   ++T + A R SPL +V   +       +L+ V + D  EF RQS+  Y++  R    + + L+ V   DHF +V +L   GY LT  I+  +
Sbjct:   22 LVLYVHGGYWQFLSKEESGFAAPPLLSRGIALVAVGYDVAPRGNMDVMVAQVRRSVACIVRQYPTTSGVYLCGHSAGAHLAAMVLATDWQE------YRVAPDIKGAFLVSGIYDLEPIVSTYVNDALRMTPEVAQRNSPLLHVAAATPATEACEVLVAVAQHDSPEFHRQSRQ-YFQALRSAGWK-VSLLDVAHTDHFDVVEKLSQEGYILTQVILSAI 233
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Cavefish
Match: afmid (arylformamidase [Source:NCBI gene;Acc:103033615])

HSP 1 Score: 82.4185 bits (202), Expect = 2.800e-17
Identity = 64/221 (28.96%), Postives = 109/221 (49.32%), Query Frame = 3 
Query:  276 LVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPDQN-VYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRF-VKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYL 932
            L+IY+HGG W  L    S F+A   V      VA+G+   P  ++  M+ QV+ ++ ++   +   N +Y+ GHS+GAHL    L  DW D        +   ++G  L+ G +DL  I  +  N   ++T + A R SP + V  ++       I++ V + D  EF++QS++ +  ++   +K  F     V   DHF I+ QL +  Y LT  ++  +
Sbjct:   79 LIIYLHGGYWQFLSKEESGFMAVPLVQKGVVVVAVGYSIAPKGNMDLMVAQVRRSVVSVIQQYSHINGLYLCGHSAGAHLAAMVLSTDWADF------GVTSPIKGAFLVSGIYDLLPILSTYVNEPLKMTEEVALRNSPSRLVPQLTASSSACEIVVAVGQNDSPEFRKQSEDYFKSLETAGLKVAFE---DVPNTDHFNIIEQLVDDNYHLTQLLLKMM 290
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Sea Lamprey
Match: afmid (arylformamidase [Source:NCBI gene;Acc:550372])

HSP 1 Score: 83.9593 bits (206), Expect = 9.159e-19
Identity = 68/250 (27.20%), Postives = 115/250 (46.00%), Query Frame = 3 
Query:  180 KKLKYETFSYNYKNMSDENKYEFFPSK-HESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEF-LHLITVDGVDHFTIVSQLGNPGYFLTDFI 920
            K +       +Y    DE    + P K + +  + +Y+HGG W  L    S ++    V +    VA+ +   P  S+  M+ Q++ A+  I   +     +Y+ GHS+GAHL    L  DW    S     +   ++G  L+ G FD+R + ++  N   ++T ++  R SP+  V+ ++       I++ V E D  EF RQS+ L   +K      F +  + V  VDHF +V +  +  Y LT  I
Sbjct:    5 KAVAETILDVSYGPGDDEKMDIYMPLKPNPAPPVFVYIHGGYWRILGKEFSGYMVPPLVESGAVVVALSYTLSPKGSMDLMVDQMRRAVAFISRQYSHFSGIYLCGHSAGAHLAAMVLATDW----SLPEYGVTPDIKGAFLLSGVFDMRPLVQTYVNEHLKMTLEDMARNSPMLKVDAMAPLAGKCHIVLAVAEHDTTEFHRQSRELEQSLK---GAGFNVTFLDVPNVDHFDLVEKFCSEEYQLTQVI 247
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Nematostella
Match: EDO43496 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYC1])

HSP 1 Score: 60.4622 bits (145), Expect = 2.260e-10
Identity = 43/176 (24.43%), Postives = 85/176 (48.30%), Query Frame = 3 
Query:  372 VAIGFDPCPTISISEMIVQVKLAIKTIFTNHPDQNVYIIGHSSGAHL-TLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSP-LQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGN 893
              I +D  PT+++ E++ Q K A++ I    P+  +Y+ GHS+GAHL  +   + DW +         ++R++G+ L+ G +DL+ +          +T   A R SP +    +  ++R    + +++ E +   F +Q+++    +         H   V G+DHF+ VS   +
Sbjct:   80 AVIDYDLLPTVTLDEIVAQTKEALQFIAKKFPNSRLYLGGHSAGAHLVAMMMTIHDWANP------KFLDRIKGVCLMAGIYDLQPLLPIRVLHDRNITRTIAIRNSPDVILRTHPPSFR--CPVKLIIGEHEPPSFHKQTRDFMQVLDSQHVSTCFH--EVSGMDHFSAVSAYAD 245
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Medaka
Match: afmid (arylformamidase [Source:NCBI gene;Acc:101157840])

HSP 1 Score: 98.5969 bits (244), Expect = 6.574e-23
Identity = 69/221 (31.22%), Postives = 111/221 (50.23%), Query Frame = 3 
Query:  273 NLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYL 932
            NLVIY+HGG W  L    S FLA   +      VA+G+D  P  ++  M+ QV+ ++ +I   +   + +Y+ GHS+GAHL    L  DW   S N       +++G  L+ G +DL  I  +  N   ++T   A R SP Q V  +        I++ V E D  EF++QS+  Y  ++    +  + L  V   DHF++V Q+ +P + LT  ++  +
Sbjct:   77 NLVIYIHGGYWQFLSKEESGFLAVPLIEKGVVVVAVGYDIAPKGNMDLMVSQVRRSVVSIVQQYSHIRGLYLCGHSAGAHLAAMVLSTDWSQYSLN------PQIKGAFLVSGIYDLLPILPTYINEPLKMTEAVAARNSPSQLVSQLQVSSPSCQIIVAVAENDSPEFRKQSEEYYTTLRAAGLK--VSLEDVPDTDHFSVVDQMVDPEFCLTKLLLKMM 289
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000069978.1 (SMESG000069978.1)

HSP 1 Score: 531.561 bits (1368), Expect = 0.000e+0
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 3 
Query:  117 MDQSLRKKFLEFIANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSKHESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPDQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYLRD 938
            MDQSLRKKFLEFIANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSKHESNNLVIYVHGGMWINLDLSSSSFLADFFVSNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPDQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYLRD
Sbjct:    1 MDQSLRKKFLEFIANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSKHESNNLVIYVHGGMWINLDLSSSSFLADFFVSNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPDQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYLRD 274
[Close]
BLAST of Abhydrolase_3 domain-containing protein vs. Planmine SMEST
Match: SMESG000070097.1 (SMESG000070097.1)

HSP 1 Score: 154.451 bits (389), Expect = 1.278e-43
Identity = 87/265 (32.83%), Postives = 161/265 (60.75%), Query Frame = 3 
Query:  144 LEFIANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSKHESNNLVIYVHGGMWINXXXXXXXXXXXXXXXNNYNFVAIGFDPCPTISISEMIVQVKLAIKTIFTNHPD-QNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQSMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENISNYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDHFTIVSQLGNPGYFLTDFIIDYLR 935
            ++ ++ KS D  K L++ET  Y   N ++EN++ FF S   S  L+I++HGG W  LDL SS FLA+FFV  N NF +IG++ CP++S+  ++V+++  +K I  ++P+   +Y+ GHS GAHL L +++ D  +   + S+    +L+ ++LI G +D+R + ++  N   +L+ ++AW+ SP++ ++ I  +  +  +L+ V E D   F+ Q+     ++  +VK E   +I     DHF++++ +G   Y + + I+D+LR
Sbjct:   30 IKVLSEKSIDNMKNLQHETKYYAGMNSTEENRFHFFHSNKISKKLIIFIHGGYWQLLDLDSSVFLANFFVEKNCNFASIGYNLCPSVSMKTILVEIRKGLKAIIDSYPEIDEIYLSGHSCGAHLALMAIIFDGFENELSLSKDDERKLKALILISGIYDIRPLCQTGENINLKLSLEDAWKFSPIRCIDRIEKFAPEFRVLVFVAEFDSPAFKEQADKFINKIINYVKSEKYLIIN---KDHFSLINDIGEKDYLINNIILDFLR 291
The following BLAST results are available for this feature:
BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
AFMID1.084e-1929.26arylformamidase [Source:HGNC Symbol;Acc:HGNC:20910... [more]
AFMID4.024e-1928.14arylformamidase [Source:HGNC Symbol;Acc:HGNC:20910... [more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
afmd-11.581e-822.75ArylForMamiDase [Source:UniProtKB/TrEMBL;Acc:Q189... [more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
KFase9.163e-625.12gene:FBgn0031821 transcript:FBtr0079265[more]
KFase9.163e-625.12gene:FBgn0031821 transcript:FBtr0339785[more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cant1b1.345e-1829.26calcium activated nucleotidase 1b [Source:ZFIN;Acc... [more]
afmid1.345e-1829.26arylformamidase [Source:NCBI gene;Acc:550372][more]
afmid1.812e-1829.26arylformamidase [Source:NCBI gene;Acc:550372][more]
afmid3.094e-1829.82arylformamidase [Source:NCBI gene;Acc:550372][more]
afmid1.565e-1629.49arylformamidase [Source:NCBI gene;Acc:550372][more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
afmid2.029e-2127.48arylformamidase [Source:NCBI gene;Acc:100127853][more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Afmid1.749e-2025.57arylformamidase [Source:MGI Symbol;Acc:MGI:2448704... [more]
Afmid2.528e-1925.60arylformamidase [Source:MGI Symbol;Acc:MGI:2448704... [more]
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BLAST of Abhydrolase_3 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q8K4H1|KFA_MOUSE1.223e-1925.57Kynurenine formamidase OS=Mus musculus OX=10090 GN... [more]
sp|Q63HM1|KFA_HUMAN5.205e-1929.26Kynurenine formamidase OS=Homo sapiens OX=9606 GN=... [more]
sp|B5XB27|KFA_SALSA1.917e-1831.22Kynurenine formamidase OS=Salmo salar OX=8030 GN=a... [more]
sp|Q566U4|KFA_DANRE8.970e-1829.26Kynurenine formamidase OS=Danio rerio OX=7955 GN=a... [more]
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BLAST of Abhydrolase_3 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3KC921.730e-2730.26Kynurenine formamidase OS=Lingula unguis OX=7574 G... [more]
A0A1S3KCT54.159e-2731.06Kynurenine formamidase OS=Lingula unguis OX=7574 G... [more]
T1EP926.976e-2631.14Abhydrolase_3 domain-containing protein OS=Helobde... [more]
A0A0B6YHH58.401e-2331.13Abhydrolase_3 domain-containing protein (Fragment)... [more]
A0A3Q0FPC11.019e-2232.27kynurenine formamidase OS=Alligator sinensis OX=38... [more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
afmid2.800e-1728.96arylformamidase [Source:NCBI gene;Acc:103033615][more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
afmid9.159e-1927.20arylformamidase [Source:NCBI gene;Acc:550372][more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO434962.260e-1024.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
afmid6.574e-2331.22arylformamidase [Source:NCBI gene;Acc:101157840][more]
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BLAST of Abhydrolase_3 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000069978.10.000e+0100.00SMESG000069978.1[more]
SMESG000070097.11.278e-4332.83SMESG000070097.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004072 ID=SMED30004072|Name=Abhydrolase_3 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1262bp
CTCATACTTTTGTTATTAAAATTAAAATGTAAATTTTTAGAATTTTCCGT
TTGCTGCAGGCTTTATTTGAACTATATTTAATAGTCAATTACAAAGAAAT
AAAATAATTTTCAAAGATGGATCAATCTCTGCGGAAGAAGTTTTTAGAGT
TTATTGCTAATAAAAGCAATGATATGAAAAAGAAACTGAAATATGAAACA
TTTTCTTATAATTACAAAAATATGAGCGACGAAAATAAATATGAATTTTT
TCCTTCTAAACATGAATCTAATAATTTAGTTATATATGTTCACGGGGGAA
TGTGGATTAATTTGGATTTATCCTCATCTTCATTTCTTGCTGATTTTTTC
GTAAGCAATAATTATAATTTTGTGGCCATTGGTTTTGATCCTTGCCCAAC
AATATCTATTTCAGAAATGATTGTTCAAGTTAAGTTAGCTATCAAAACAA
TTTTCACAAACCATCCAGATCAGAATGTTTATATAATTGGTCACTCATCT
GGTGCTCATTTGACTTTGTCATCTCTACTCTGTGACTGGCTAGATGAGAG
TTCGAATTTTTCCCAGTCAATGATTGAAAGATTGCGTGGAATTGTATTAA
TTGGAGGCACATTTGATCTGAGGGAAATTTATAAATCTCCTTCCAACAGC
CTTTGGCAATTAACAAGCGATGAAGCATGGAGAGTCAGTCCGCTGCAATA
TGTGGAAAATATTTCCAATTATCGACATGATTTAATGATTTTGATTCTTG
TTGAAGAAATTGATTACCGAGAGTTTCAAAGGCAGTCGAAAAATCTGTAT
TACGAAATGAAAAGATTTGTTAAAGAAGAATTTTTACATTTGATAACTGT
TGATGGAGTTGATCATTTCACAATTGTCAGCCAGTTGGGCAATCCCGGTT
ATTTTTTGACAGATTTTATCATTGATTATCTCAGGGATTGAGCGCAATTT
CATAAATTGACTTTGTTATTTCTTTATTAATGTGATCGATATAATTATTT
ATCGGCGTTGTTGTGACAACCAAATTGTATCGATTTCTAATTACTTGTAC
TAAATTATTTTTTATTAAATTATAATCCCAGAGTATTCGTCCTCGGACGA
TTGATTACTTTACGTCATCAAAGTATACGTTTAGTCTGTAAATAAATTTC
AATAAATAAATAAGCTTATTGATTAATTAGTAAAAAACATACGTTGTGAC
AACAGTAAATTTTGGAACATCGAATCGTGTAAAGAGAATCGTAAAGAAAA
CATCGAAACTAC
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protein sequence of SMED30004072-orf-1

>SMED30004072-orf-1 ID=SMED30004072-orf-1|Name=SMED30004072-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=275bp
MDQSLRKKFLEFIANKSNDMKKKLKYETFSYNYKNMSDENKYEFFPSKHE
SNNLVIYVHGGMWINLDLSSSSFLADFFVSNNYNFVAIGFDPCPTISISE
MIVQVKLAIKTIFTNHPDQNVYIIGHSSGAHLTLSSLLCDWLDESSNFSQ
SMIERLRGIVLIGGTFDLREIYKSPSNSLWQLTSDEAWRVSPLQYVENIS
NYRHDLMILILVEEIDYREFQRQSKNLYYEMKRFVKEEFLHLITVDGVDH
FTIVSQLGNPGYFLTDFIIDYLRD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000103central nervous system
PLANA:0000136whole organism
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR029058AB_hydrolase
Vocabulary: molecular function
TermDefinition
GO:0004061arylformamidase activity
GO:0016787hydrolase activity
Vocabulary: biological process
TermDefinition
GO:0006569tryptophan catabolic process
GO:0008152metabolic process
GO:0019441tryptophan catabolic process to kynurenine
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 10..270
e-value: 4.6E-40
score: 139.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 37..273
NoneNo IPR availablePANTHERPTHR23024:SF238KYNURENINE FORMAMIDASEcoord: 25..258
NoneNo IPR availablePANTHERPTHR23024MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMEScoord: 25..258