DNA_pol_B_2 domain-containing protein

Overview
NameDNA_pol_B_2 domain-containing protein
Smed IDSMED30004052
Length (bp)1351
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA_pol_B_2 domain-containing protein (SMED30004052) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA_pol_B_2 domain-containing protein (SMED30004052) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA_pol_B_2 domain-containing protein (SMED30004052) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA_pol_B_2 domain-containing protein (SMED30004052) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30004052

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Celegans
Match: ZC53.4 (pep chromosome:WBcel235:X:1925750:1937689:1 gene:WBGene00022511.1 transcript:ZC53.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC53.4)

HSP 1 Score: 53.9138 bits (128), Expect = 1.961e-7
Identity = 39/98 (39.80%), Postives = 56/98 (57.14%), Query Frame = 3 
Query: 1056 LCNQ-YGKEK*-----NLQFYLIQGMKLKTVYNIISFKQKPFMKSVIEDNIKNRKEATSNKDELGKILYKLLINFVFDKTMENIDKYVKINFLTNSHK 1331
            LCN  + +EK      NL   L  G+KLK ++ II+FK+  ++ S IE N   RK A   K++  K  YKL+ N VF KTMEN+ + V +  +T+  K
Sbjct: 1298 LCNTLFDREKYVIHYENLLQCLQLGLKLKKIHRIITFKESNWLASYIEMNTNPRKNA---KNDFEKDFYKLMNNSVFGKTMENVRERVDVRLVTDEDK 1392
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Celegans
Match: H12I13.3 (pep chromosome:WBcel235:II:6071386:6073251:-1 gene:WBGene00019192.1 transcript:H12I13.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:H12I13.3)

HSP 1 Score: 49.6766 bits (117), Expect = 3.237e-6
Identity = 32/79 (40.51%), Postives = 46/79 (58.23%), Query Frame = 3 
Query: 1101 LIQGMKLKTVYNIISFKQKPFMKSVIEDNIKNRKEATSNKDELGKILYKLLINFVFDKTMENIDKYVKINFLTNSHKDL 1337
            L  G+KLK ++ I++FK+  +MK  I  N   RK+A   K++  K  +KL+ N VF KTMENI   V I  +T+    L
Sbjct:  296 LNHGLKLKKIHRILTFKESAWMKKYIMLNTNLRKQA---KNDFEKDFFKLMNNSVFGKTMENIRNRVNIRLVTDDDSAL 371
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. TrEMBL
Match: V3ZKK6 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_121815 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 4.564e-18
Identity = 57/175 (32.57%), Postives = 92/175 (52.57%), Query Frame = 3 
Query:  219 NSGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSN 743
            +SGSGKT L   M+ TPN LD+++L I++ T +Q   Q +       L K+++  LFQ      +   + D  +   EEKK                 P  I V ++   +++    ++   +KNL++FD CV  +NQ + +  FT+GRH+NC+ IY  Q++YG+ S  IR N+N
Sbjct:   23 SSGSGKTHLTFNMLTTPNLLDFDSLYIYTTTPEQSYYQFLI----EYLPKRDVQDLFQYYKENEEKVELKDFIDNYIEEKK-----------------PTDIKVFLTKNVNDL-DLSQIDSERKNLLVFDDCVAQRNQAVQQEFFTKGRHHNCHCIYQSQSFYGMDSMFIRKNAN 175
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. TrEMBL
Match: V4C8M2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_143112 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 9.531e-18
Identity = 60/175 (34.29%), Postives = 90/175 (51.43%), Query Frame = 3 
Query:  219 NSGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSN 743
            NSGSGKT L   M+ T N LD+++L I++ T +Q   Q  F      L K+N+  LFQ      +   I D  +   E KK                 P  I V ++   +++    K+   +KNL++FD CV  +NQ + +  FT+GRH+NC+ IY  Q++YG+ S  IR N+N
Sbjct:   23 NSGSGKTHLTFNMLTTQNLLDFDSLYIYTTTPEQSYYQ--FRKALEYLPKKNVQDLFQYYAENEEEVEIKDFIDNYIEGKK-----------------PTHIKVFLTKNVNDL-DLSKIDSERKNLLVFDDCVAQRNQAVQQEFFTKGRHHNCHCIYQSQSFYGMDSMFIRKNAN 177
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. TrEMBL
Match: V4AGI4 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_144286 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.002e-17
Identity = 57/176 (32.39%), Postives = 92/176 (52.27%), Query Frame = 3 
Query:  219 NSGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSNF 746
            +SGSGKT L   M+ TPN LD+++L I++ T +Q   Q  F      L K+++  LFQ      +   + D  +   E KK                 P  I V ++   +++    ++   +KNL++FD CV  +NQ + +  FT+GRH+NC+ IY  Q++YG+ S  IR N+N+
Sbjct:   23 SSGSGKTHLTFNMLTTPNLLDFDSLCIYTTTPEQSYYQ--FLKALEYLPKKDVQDLFQYYKENEEEVELKDFIDNYIEGKK-----------------PTDIKVFLTKNVNDL-DLSQIDSERKNLLVFDDCVAQRNQAVQQEFFTKGRHHNCHCIYQSQSFYGMDSMFIRKNANY 178
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. TrEMBL
Match: V4B262 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_140907 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 2.189e-17
Identity = 59/180 (32.78%), Postives = 93/180 (51.67%), Query Frame = 3 
Query:  222 SGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSNFSYSLN 761
            SGSGKT L   M+ TPN LD+++L I++ T +Q   Q  F      L K+++ G+FQ  +   +   I D+                     +  + P  I V ++   +++     +   +KNLI+FD CV  +NQ + +  FT+GRH+NC+ IY  Q++YG+ S  IR N+N  Y LN
Sbjct:   19 SGSGKTHLTFNMLTTPNLLDFDSLYIYTTTPEQSYYQ--FLKALEYLPKKDVHGIFQYYEENEEEVEIKDI------------------DNFIKSKNPTDIKVFLTKNVNDL-DLSNIDSNRKNLILFDDCVAQRNQAVQQEFFTKGRHHNCHCIYQSQSFYGMDSMFIRKNANCFYYLN 177
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. TrEMBL
Match: V4A582 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_121719 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 3.959e-17
Identity = 60/174 (34.48%), Postives = 92/174 (52.87%), Query Frame = 3 
Query:  222 SGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSN 743
            SGSGKT L   M+ TPN LD+++L I++ T +Q   Q  F      L K+++  LFQ                  YEE +  E+  I    + G + P  I V ++   +++     +   +KNLI+FD CV  +NQ + +  FT+GRH+NC+ IY  Q++YG+ S  IR N+N
Sbjct:   24 SGSGKTHLTFNMLTTPNLLDFDSLYIYTTTPEQSYYQ--FLKALEYLPKKDVQDLFQ-----------------YYEENEEVEIKDIIDNYIEG-KNPTDIKVFLTKNVNDL-DLSNIDSNRKNLILFDDCVAQRNQTVQQEFFTKGRHHNCHCIYQSQSFYGMDSMFIRKNAN 176
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Nematostella
Match: EDO33832 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SR14])

HSP 1 Score: 85.8853 bits (211), Expect = 2.905e-19
Identity = 65/180 (36.11%), Postives = 91/180 (50.56%), Query Frame = 3 
Query:  207 LNVDNSGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSNF 746
            L V  SG GKT LLL ++L P +LD+NNL +F K++ QP+ +I+       L K+ I+ LF  QD +  N +          EK   E     +AE V P P                    L   KKNL+IFD  + ++ Q   E  + RGRH+N +  YL QNY+ LP ++IR N+NF
Sbjct:   28 LIVGKSGCGKTTLLLNLLLRPGWLDFNNLQMFGKSLFQPEYKILKKAFEEKLPKEAIIRLFDIQDEMSKNLA----------EKADMECKFFETAEDV-PDP------------------RDLNPQKKNLMIFDD-LQLEKQNKCEAYYIRGRHSNVDCFYLAQNYFKLPRQTIRENANF 177
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000073434.1 (SMESG000073434.1)

HSP 1 Score: 286.574 bits (732), Expect = 9.089e-104
Identity = 144/180 (80.00%), Postives = 159/180 (88.33%), Query Frame = 3 
Query:  207 LNVDNSGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSNF 746
            L + +SGSGKTVLLLK+ILTPNF+DY+NLIIFSKTIDQP+MQIIF+GLNSGLTKQ+IV LFQNQD IPD+TSI DLCEEL EE KCGEL   ++ E+   +P  KIGVSMSNKYHEIIPCEKLYRAKKNLIIFD CVNMKNQEI+E  FTRGRHNNCN+IYL QNYY LPSRSIRGNSNF
Sbjct:   28 LIIGSSGSGKTVLLLKLILTPNFIDYDNLIIFSKTIDQPEMQIIFYGLNSGLTKQDIVRLFQNQDKIPDDTSIRDLCEELCEETKCGEL---STGEL---KPSNKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDDCVNMKNQEIMESYFTRGRHNNCNLIYLAQNYYDLPSRSIRGNSNF 201

HSP 2 Score: 99.7525 bits (247), Expect = 9.089e-104
Identity = 50/52 (96.15%), Postives = 51/52 (98.08%), Query Frame = 2 
Query:  758 KLGKRNKDSIFNDLFSDTLDKEEFNSFENQLSIKYNYIALDKEKNQVYEKII 913
            KLGKRNKDSIFNDLFSDTLDKEEFNSFEN LSIKYNYIALDKEKN+VYEKII
Sbjct:  206 KLGKRNKDSIFNDLFSDTLDKEEFNSFENHLSIKYNYIALDKEKNKVYEKII 257

HSP 3 Score: 32.7278 bits (73), Expect = 9.089e-104
Identity = 15/20 (75.00%), Postives = 16/20 (80.00%), Query Frame = 2 
Query:  134 MYSFTPTGNKNIMKFNHNSI 193
            M SFT T NKNI+KFNHN I
Sbjct:    1 MSSFTTTWNKNILKFNHNPI 20
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000001725.1 (SMESG000001725.1)

HSP 1 Score: 289.271 bits (739), Expect = 7.115e-92
Identity = 139/161 (86.34%), Postives = 147/161 (91.30%), Query Frame = 3 
Query:  279 DYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSNFSYSLN 761
            +Y+NLIIFSKT DQP+MQII+HGLN GLTKQ+IVGLFQNQD IPD+TSI DLCEELYE+ KCGELNSITSAEMV  RPPQKIGVSMSNKYHEIIPCE L RAKKNLIIFD CVNMKNQEI+E  FTRGRHNNCNVIYL QNYY LPSRSIRGNSNFSYSLN
Sbjct:  397 NYDNLIIFSKTTDQPEMQIIYHGLNFGLTKQDIVGLFQNQDKIPDDTSIRDLCEELYEKTKCGELNSITSAEMVDQRPPQKIGVSMSNKYHEIIPCENLNRAKKNLIIFDDCVNMKNQEIMESYFTRGRHNNCNVIYLAQNYYDLPSRSIRGNSNFSYSLN 557
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000001725.1 (SMESG000001725.1)

HSP 1 Score: 289.271 bits (739), Expect = 7.286e-92
Identity = 139/161 (86.34%), Postives = 147/161 (91.30%), Query Frame = 3 
Query:  279 DYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSNFSYSLN 761
            +Y+NLIIFSKT DQP+MQII+HGLN GLTKQ+IVGLFQNQD IPD+TSI DLCEELYE+ KCGELNSITSAEMV  RPPQKIGVSMSNKYHEIIPCE L RAKKNLIIFD CVNMKNQEI+E  FTRGRHNNCNVIYL QNYY LPSRSIRGNSNFSYSLN
Sbjct:  402 NYDNLIIFSKTTDQPEMQIIYHGLNFGLTKQDIVGLFQNQDKIPDDTSIRDLCEELYEKTKCGELNSITSAEMVDQRPPQKIGVSMSNKYHEIIPCENLNRAKKNLIIFDDCVNMKNQEIMESYFTRGRHNNCNVIYLAQNYYDLPSRSIRGNSNFSYSLN 562
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000014504.1 (SMESG000014504.1)

HSP 1 Score: 276.559 bits (706), Expect = 1.033e-89
Identity = 141/179 (78.77%), Postives = 150/179 (83.80%), Query Frame = 3 
Query:  204 FLNVDNSGSGKTVLLLKMILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNS 740
             L + +SGSGKTVLLLKMILTPNF+DY+NLIIFSKTIDQP+MQIIF  LNSGLTKQ+IVGLFQNQD IPD+TSI DLCEEL EE KC EL SI            KIGVSMSNKYHEIIPCEKL RAK NLIIFDYCVNMKNQEI+E  FTRGRHNNCNVIYL QNYY LPSRSIRGN+
Sbjct:   32 MLIIGSSGSGKTVLLLKMILTPNFIDYDNLIIFSKTIDQPEMQIIFQWLNSGLTKQDIVGLFQNQDKIPDDTSIRDLCEELCEETKCVELKSI-----------NKIGVSMSNKYHEIIPCEKLDRAKNNLIIFDYCVNMKNQEIIESYFTRGRHNNCNVIYLAQNYYDLPSRSIRGNA 199
[Close]
BLAST of DNA_pol_B_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000059568.1 (SMESG000059568.1)

HSP 1 Score: 237.654 bits (605), Expect = 1.560e-83
Identity = 121/153 (79.08%), Postives = 128/153 (83.66%), Query Frame = 3 
Query:  327 MQIIFHGLNSGLTKQNIVGLFQNQDVIPDNTSISDLCEELYEEKKC------------GELNSITSA-EMVGPRPPQKIGVSMSNKYHEIIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNYYGLPSRSIRGNSNF 746
            MQIIFHGLNSGL+KQ+IV LFQ+QDVIPD+TSI DLCEELYEE KC            GELNSITS+ EMVG RPPQK GVSMSNKYHEIIPCEKL RAKKNLIIF+ CVNMKNQEI+E  FTRGRHNNCNVIYL QNYY LP RSIRGNSNF
Sbjct:    1 MQIIFHGLNSGLSKQDIVSLFQSQDVIPDDTSIRDLCEELYEETKCEAISCRAQANAFGELNSITSSVEMVGRRPPQKFGVSMSNKYHEIIPCEKLDRAKKNLIIFNDCVNMKNQEIMESYFTRGRHNNCNVIYLTQNYYDLPFRSIRGNSNF 153

HSP 2 Score: 92.0485 bits (227), Expect = 1.560e-83
Identity = 46/52 (88.46%), Postives = 49/52 (94.23%), Query Frame = 2 
Query:  758 KLGKRNKDSIFNDLFSDTLDKEEFNSFENQLSIKYNYIALDKEKNQVYEKII 913
            KLGKRN++SIFNDL SDTLDKEEFNSF+N LSIKYNYI LDKEKNQVYEKII
Sbjct:  158 KLGKRNRNSIFNDLSSDTLDKEEFNSFDNNLSIKYNYIVLDKEKNQVYEKII 209
The following BLAST results are available for this feature:
BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ZC53.41.961e-739.80pep chromosome:WBcel235:X:1925750:1937689:1 gene:W... [more]
H12I13.33.237e-640.51pep chromosome:WBcel235:II:6071386:6073251:-1 gene... [more]
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V3ZKK64.564e-1832.57Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4C8M29.531e-1834.29Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4AGI42.002e-1732.39Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
V4B2622.189e-1732.78Uncharacterized protein (Fragment) OS=Lottia gigan... [more]
V4A5823.959e-1734.48Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO338322.905e-1936.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA_pol_B_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA_pol_B_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000073434.19.089e-10480.00SMESG000073434.1[more]
SMESG000001725.17.115e-9286.34SMESG000001725.1[more]
SMESG000001725.17.286e-9286.34SMESG000001725.1[more]
SMESG000014504.11.033e-8978.77SMESG000014504.1[more]
SMESG000059568.11.560e-8379.08SMESG000059568.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30004052 ID=SMED30004052|Name=DNA_pol_B_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1351bp
TAAAAAATTAAACCAAGTTTTGAAAAAATTAAATAAAGGAAATATTCAAA
TAGAGGTTGATGGTGATGGAATTTTTCTTTCTCCTTATAAAAAAATAAAT
AATTTTTCATTATTAAAGATTTTACTATTAAAAATGTATTCGTTTACACC
AACTGGGAATAAAAATATTATGAAATTCAATCACAATTCTATTTGCCAAA
TTCTTTTTGAATGTTGATAATTCTGGTTCTGGAAAAACAGTTTTGTTGCT
AAAAATGATTCTAACTCCTAATTTTTTAGACTATAATAATTTAATAATTT
TTTCTAAAACAATTGATCAACCTAAAATGCAAATAATTTTTCATGGGTTA
AATTCTGGTTTAACAAAACAAAATATTGTGGGATTATTTCAAAATCAAGA
TGTAATTCCCGATAATACATCTATTAGCGATTTATGTGAAGAATTATATG
AAGAAAAAAAATGTGGGGAATTAAACTCTATAACTTCTGCAGAAATGGTC
GGCCCTAGGCCTCCTCAAAAAATTGGTGTATCAATGAGTAATAAATATCA
TGAAATAATTCCATGTGAAAAATTATATCGAGCGAAGAAGAATTTAATAA
TTTTTGATTATTGTGTCAACATGAAAAATCAAGAAATTTTAGAATTTGAT
TTTACTCGAGGTAGACATAATAATTGCAATGTAATTTATTTAGGTCAAAA
TTATTATGGTTTACCATCTAGAAGTATTCGCGGTAATTCTAATTTTTCAT
ATTCTTTAAATTAGGAAAAAGAAATAAAGATAGTATTTTTAATGATTTGT
TTAGTGATACTTTAGACAAGGAAGAATTTAATAGTTTTGAAAATCAGCTA
TCTATAAAATATAATTATATTGCATTGGATAAAGAAAAAAATCAAGTATA
TGAAAAAATAATTAGTTAATCAATTTAAAATGAGTAGAAATGTAAATGAT
TTTAATCTTCAAAAAATATATTCGAGATTGTAAATTATTAAATATTCTAC
AAGATATTTGGAATGAAAATTTAATATCTTAAAGACTGTATGATCAAAAC
TTTAACTATGTAATCAATATGGTAAAGAAAAATAAAATTTACAATTTTAT
TTAATACAAGGAATGAAATTAAAAACAGTTTATAATATTATTAGTTTTAA
ACAGAAACCATTCATGAAAAGTGTTATTGAAGATAATATTAAAAATAGAA
AAGAAGCAACATCGAACAAGGATGAATTAGGAAAAATATTGTATAAATTA
TTAATTAATTTTGTCTTTGATAAAACTATGGAAAATATTGATAAATATGT
AAAAATCAATTTTTTAACAAATAGTCATAAAGATTTAATAAAATATTCTA
A
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protein sequence of SMED30004052-orf-1

>SMED30004052-orf-1 ID=SMED30004052-orf-1|Name=SMED30004052-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=170bp
MILTPNFLDYNNLIIFSKTIDQPKMQIIFHGLNSGLTKQNIVGLFQNQDV
IPDNTSISDLCEELYEEKKCGELNSITSAEMVGPRPPQKIGVSMSNKYHE
IIPCEKLYRAKKNLIIFDYCVNMKNQEILEFDFTRGRHNNCNVIYLGQNY
YGLPSRSIRGNSNFSYSLN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0003676nucleic acid binding
GO:0003677DNA binding
GO:0003887DNA-directed DNA polymerase activity
Vocabulary: biological process
TermDefinition
GO:0006260DNA replication
GO:0071897DNA biosynthetic process
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland