SMED30003997

Overview


Name	SMED30003997
Smed ID	SMED30003997
Length (bp)	669

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30003997 (SMED30003997) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003997 (SMED30003997) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003997 (SMED30003997) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003997 (SMED30003997) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30003997

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_244_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_244_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
pharynx	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_483_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_483_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_483_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_483_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_483_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_483_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30003997	SMESG000059318.1 SMESG000059304.1	dd_Smed_v4_483_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30003997 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000393:3628..4368 +	3228

Homology

[Close]

BLAST of SMED30003997 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 67.781 bits (164), Expect = 1.353e-13
Identity = 51/187 (27.27%), Postives = 74/187 (39.57%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK-------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                 ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQE----------------------------------------------DVEACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 128

HSP 2 Score: 59.6918 bits (143), Expect = 1.226e-10
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C   +         ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   47 CYRCGESGHLAKDCDLQE------DVEACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 167

HSP 3 Score: 46.9802 bits (110), Expect = 4.092e-6
Identity = 25/80 (31.25%), Postives = 41/80 (51.25%), Query Frame = 1

Query:  376 CYSCGQSGHISSYCPQGSRNNDSRNRGS--YKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            C+ CG+SGH +  CP G             ++F   +   + CY C ++GH++++C      + CY C   GHIA+DC +
Sbjct:    6 CFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDI-CYRCGESGHLAKDCDLQEDVEACYNCGRGGHIAKDCKE 84

[Close]

BLAST of SMED30003997 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 67.781 bits (164), Expect = 1.472e-13
Identity = 51/187 (27.27%), Postives = 74/187 (39.57%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK-------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                 ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 126

HSP 2 Score: 60.077 bits (144), Expect = 9.204e-11
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   47 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 165

[Close]

BLAST of SMED30003997 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 65.4698 bits (158), Expect = 1.148e-12
Identity = 51/194 (26.29%), Postives = 75/194 (38.66%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                        ++ S S P  CY CGE+GH++KDC   E  +                                               CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDVE----------------------------------------------ACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 135

HSP 2 Score: 60.077 bits (144), Expect = 1.160e-10
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C   +         ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   54 CYRCGESGHLAKDCDLQE------DVEACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 174

[Close]

BLAST of SMED30003997 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 65.0846 bits (157), Expect = 1.304e-12
Identity = 51/194 (26.29%), Postives = 74/194 (38.14%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                        ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 133

HSP 2 Score: 60.077 bits (144), Expect = 9.175e-11
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   54 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 172

[Close]

BLAST of SMED30003997 vs. Ensembl Human
Match: CNBP (CCHC-type zinc finger nucleic acid binding protein [Source:HGNC Symbol;Acc:HGNC:13164])

HSP 1 Score: 65.0846 bits (157), Expect = 1.369e-12
Identity = 51/194 (26.29%), Postives = 74/194 (38.14%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                        ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDE-----------------------------------------------ACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 134

HSP 2 Score: 63.1586 bits (152), Expect = 6.774e-12
Identity = 49/178 (27.53%), Postives = 69/178 (38.76%), Query Frame = 1

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            Q    CYNCG+ GH +++C E KR     +   CYNCG+ GH+++DC   + +                                                CYSCG+ GHI   C                       +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   70 QEDEACYNCGRGGHIAKDCKEPKR----EREQCCYNCGKPGHLARDCDHADEQK-----------------------------------------------CYSCGEFGHIQKDCT----------------------KVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 173

[Close]

BLAST of SMED30003997 vs. Ensembl Celegans
Match: K08D12.3 (pep chromosome:WBcel235:IV:1710265:1711127:1 gene:WBGene00019537.1 transcript:K08D12.3a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:K08D12.3)

HSP 1 Score: 52.7582 bits (125), Expect = 1.219e-8
Identity = 32/71 (45.07%), Postives = 39/71 (54.93%), Query Frame = 1

Query:  397 GHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPS--EKKCYKCQNTGHIARDC 603
            GH S  CP       S   G       +    KCYNC ++GH+SREC    S  EK+CY+CQ TGHI+RDC
Sbjct:   76 GHYSRDCPSARSEGGSGGYGGRGGEGRSFGGQKCYNCGRSGHISRECTESGSAEEKRCYQCQETGHISRDC 146

HSP 2 Score: 49.2914 bits (116), Expect = 1.934e-7
Identity = 25/44 (56.82%), Postives = 30/44 (68.18%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCP 216
            +CYNCG+ GH SRECTES      ++   CY C E GHIS+DCP
Sbjct:  108 KCYNCGRSGHISRECTES----GSAEEKRCYQCQETGHISRDCP 147

HSP 3 Score: 45.0542 bits (105), Expect = 6.273e-6
Identity = 23/45 (51.11%), Postives = 26/45 (57.78%), Query Frame = 1

Query:  496 CYNCNQNGHMSRECPNGPSE---------KKCYKCQNTGHIARDC 603
            CY C Q GH+SR CPNG S+           CY CQ TGH +RDC
Sbjct:    6 CYKCQQPGHISRNCPNGESDGGRRGGGGGSTCYNCQETGHFSRDC 50

[Close]

BLAST of SMED30003997 vs. Ensembl Fly
Match: CNBP (gene:FBgn0034802 transcript:FBtr0303464)

HSP 1 Score: 59.6918 bits (143), Expect = 4.799e-11
Identity = 48/181 (26.52%), Postives = 64/181 (35.36%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            M + R +CY C Q GH++R C E            CY C   GHISKDC Q +                                                  CY C ++GH    CP+        N             V CY CN+ GH+S+ CP   + K CY C  +GH+ R+C +
Sbjct:   50 MRRNREKCYKCNQFGHFARACPEEAE--------RCYRCNGIGHISKDCTQADNP-----------------------------------------------TCYRCNKTGHWVRNCPEAVNERGPTN-------------VSCYKCNRTGHISKNCPE--TSKTCYGCGKSGHLRRECDE 160

[Close]

BLAST of SMED30003997 vs. Ensembl Fly
Match: CNBP (gene:FBgn0034802 transcript:FBtr0071994)

HSP 1 Score: 59.6918 bits (143), Expect = 4.799e-11
Identity = 48/181 (26.52%), Postives = 64/181 (35.36%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            M + R +CY C Q GH++R C E            CY C   GHISKDC Q +                                                  CY C ++GH    CP+        N             V CY CN+ GH+S+ CP   + K CY C  +GH+ R+C +
Sbjct:   50 MRRNREKCYKCNQFGHFARACPEEAE--------RCYRCNGIGHISKDCTQADNP-----------------------------------------------TCYRCNKTGHWVRNCPEAVNERGPTN-------------VSCYKCNRTGHISKNCPE--TSKTCYGCGKSGHLRRECDE 160

[Close]

BLAST of SMED30003997 vs. Ensembl Zebrafish
Match: cnbpb (CCHC-type zinc finger, nucleic acid binding protein b [Source:ZFIN;Acc:ZDB-GENE-030131-7782])

HSP 1 Score: 62.7734 bits (151), Expect = 4.560e-12
Identity = 49/175 (28.00%), Postives = 75/175 (42.86%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            CY CG+ GH +R+C  ++         ACYNCG  GHIS+DC + ++   +                                            VCY+CG++GH++  C   +        G      G +K VKCY C + GH++ +C    SE  CY C  +GH+A++CT +
Sbjct:   38 CYRCGEPGHVARDCERTE--------DACYNCGRGGHISRDCKEPKKEREQ--------------------------------------------VCYNCGKAGHMARDCDHANEQKCYSCGGFGHIQKGCEK-VKCYRCGEIGHVAVQCSK-ASEVNCYNCGKSGHVAKECTIE 158

HSP 2 Score: 53.1434 bits (126), Expect = 1.384e-8
Identity = 45/177 (25.42%), Postives = 69/177 (38.98%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESKRYGSGSKPGA----CYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDC 603
            EC+ CG+ GH+ + C  + R     +       CY CGE GH+++DC + E                                                  CY+CG+ GHIS  C +  +                +++  CYNC + GHM+R+C +  +E+KCY C   GHI + C
Sbjct:    5 ECFGCGRTGHWIKNCPNAGRGRGKGRGRGKDLFCYRCGEPGHVARDCERTED------------------------------------------------ACYNCGRGGHISRDCKEPKK----------------EREQVCYNCGKAGHMARDCDH-ANEQKCYSCGGFGHIQKGC 116

[Close]

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            CY CG+ GH +R+C  ++         ACYNCG  GHIS+DC + ++   +                                            VCY+CG++GH++  C   +        G      G +K VKCY C + GH++ +C    SE  CY C  +GH+A++CT +
Sbjct:   38 CYRCGEPGHVARDCERTE--------DACYNCGRGGHISRDCKEPKKEREQ--------------------------------------------VCYNCGKAGHMARDCDHANEQKCYSCGGFGHIQKGCEK-VKCYRCGEIGHVAVQCSK-ASEVNCYNCGKSGHVAKECTIE 158

HSP 2 Score: 53.1434 bits (126), Expect = 1.384e-8
Identity = 45/177 (25.42%), Postives = 69/177 (38.98%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESKRYGSGSKPGA----CYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDC 603
            EC+ CG+ GH+ + C  + R     +       CY CGE GH+++DC + E                                                  CY+CG+ GHIS  C +  +                +++  CYNC + GHM+R+C +  +E+KCY C   GHI + C
Sbjct:    5 ECFGCGRTGHWIKNCPNAGRGRGKGRGRGKDLFCYRCGEPGHVARDCERTED------------------------------------------------ACYNCGRGGHISRDCKEPKK----------------EREQVCYNCGKAGHMARDCDH-ANEQKCYSCGGFGHIQKGC 116

[Close]

BLAST of SMED30003997 vs. Ensembl Zebrafish
Match: cnbpa (CCHC-type zinc finger, nucleic acid binding protein a [Source:ZFIN;Acc:ZDB-GENE-030131-5045])

HSP 1 Score: 61.6178 bits (148), Expect = 1.499e-11
Identity = 49/178 (27.53%), Postives = 66/178 (37.08%), Query Frame = 1

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            Q    CYNC + GH SR+C E K+     +   CYNCG+ GH+++DC     +                                                CYSCG  GHI   C                       +VKCY C + GH++ +C    +E  CY C  TGH+ARDC+
Sbjct:   55 QTEDACYNCHRSGHISRDCKEPKK----EREQCCYNCGKAGHVARDCDHANEQK-----------------------------------------------CYSCGGFGHIQKLCD----------------------KVKCYRCGEIGHVAVQCSK-ATEVNCYNCGKTGHLARDCS 158

HSP 2 Score: 47.7506 bits (112), Expect = 1.194e-6
Identity = 27/78 (34.62%), Postives = 38/78 (48.72%), Query Frame = 1

Query:  376 CYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            C+ CG+SGH    CP   R                 K + CY C + GH++R+C    +E  CY C  +GHI+RDC +
Sbjct:    8 CFGCGRSGHWIKNCPNAGRGRGRGRGRG--------KDLFCYRCGEQGHIARDCEQ--TEDACYNCHRSGHISRDCKE 75

[Close]

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            Q    CYNC + GH SR+C E K+     +   CYNCG+ GH+++DC     +                                                CYSCG  GHI   C                       +VKCY C + GH++ +C    +E  CY C  TGH+ARDC+
Sbjct:   55 QTEDACYNCHRSGHISRDCKEPKK----EREQCCYNCGKAGHVARDCDHANEQK-----------------------------------------------CYSCGGFGHIQKLCD----------------------KVKCYRCGEIGHVAVQCSK-ATEVNCYNCGKTGHLARDCS 158

HSP 2 Score: 47.7506 bits (112), Expect = 1.194e-6
Identity = 27/78 (34.62%), Postives = 38/78 (48.72%), Query Frame = 1

Query:  376 CYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            C+ CG+SGH    CP   R                 K + CY C + GH++R+C    +E  CY C  +GHI+RDC +
Sbjct:    8 CFGCGRSGHWIKNCPNAGRGRGRGRGRG--------KDLFCYRCGEQGHIARDCEQ--TEDACYNCHRSGHISRDCKE 75

[Close]

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            Q    CYNC + GH SR+C E K+     +   CYNCG+ GH+++DC     +                                                CYSCG  GHI   C                       +VKCY C + GH++ +C    +E  CY C  TGH+ARDC+
Sbjct:   55 QTEDACYNCHRSGHISRDCKEPKK----EREQCCYNCGKAGHVARDCDHANEQK-----------------------------------------------CYSCGGFGHIQKLCD----------------------KVKCYRCGEIGHVAVQCSK-ATEVNCYNCGKTGHLARDCS 158

HSP 2 Score: 47.7506 bits (112), Expect = 1.194e-6
Identity = 27/78 (34.62%), Postives = 38/78 (48.72%), Query Frame = 1

Query:  376 CYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            C+ CG+SGH    CP   R                 K + CY C + GH++R+C    +E  CY C  +GHI+RDC +
Sbjct:    8 CFGCGRSGHWIKNCPNAGRGRGRGRGRG--------KDLFCYRCGEQGHIARDCEQ--TEDACYNCHRSGHISRDCKE 75

[Close]

BLAST of SMED30003997 vs. Ensembl Mouse
Match: Cnbp (cellular nucleic acid binding protein [Source:MGI Symbol;Acc:MGI:88431])

HSP 1 Score: 67.781 bits (164), Expect = 1.011e-13
Identity = 51/187 (27.27%), Postives = 74/187 (39.57%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK-------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                 ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 126

HSP 2 Score: 60.077 bits (144), Expect = 6.319e-11
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   47 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 165

[Close]

BLAST of SMED30003997 vs. Ensembl Mouse
Match: Cnbp (cellular nucleic acid binding protein [Source:MGI Symbol;Acc:MGI:88431])

HSP 1 Score: 67.0106 bits (162), Expect = 1.653e-13
Identity = 51/188 (27.13%), Postives = 74/188 (39.36%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                  ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 127

HSP 2 Score: 60.077 bits (144), Expect = 6.831e-11
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   48 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 166

[Close]

BLAST of SMED30003997 vs. Ensembl Mouse
Match: Cnbp (cellular nucleic acid binding protein [Source:MGI Symbol;Acc:MGI:88431])

HSP 1 Score: 65.0846 bits (157), Expect = 8.952e-13
Identity = 51/194 (26.29%), Postives = 74/194 (38.14%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                        ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 133

HSP 2 Score: 60.077 bits (144), Expect = 6.299e-11
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   54 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 172

[Close]

Query:   85 ECYNCGQDGHYSRECTESK--------------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                        ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 133

HSP 2 Score: 60.077 bits (144), Expect = 6.299e-11
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   54 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 172

[Close]

BLAST of SMED30003997 vs. Ensembl Mouse
Match: Cnbp (cellular nucleic acid binding protein [Source:MGI Symbol;Acc:MGI:88431])

HSP 1 Score: 65.0846 bits (157), Expect = 9.399e-13
Identity = 51/194 (26.29%), Postives = 74/194 (38.14%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                        ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQEDE-----------------------------------------------ACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 134

HSP 2 Score: 63.1586 bits (152), Expect = 4.650e-12
Identity = 49/178 (27.53%), Postives = 69/178 (38.76%), Query Frame = 1

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            Q    CYNCG+ GH +++C E KR     +   CYNCG+ GH+++DC   + +                                                CYSCG+ GHI   C                       +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   70 QEDEACYNCGRGGHIAKDCKEPKR----EREQCCYNCGKPGHLARDCDHADEQK-----------------------------------------------CYSCGEFGHIQKDCT----------------------KVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 173

[Close]

BLAST of SMED30003997 vs. UniProt/SwissProt
Match: sp|Q04832|HEXP_LEIMA (DNA-binding protein HEXBP OS=Leishmania major OX=5664 GN=HEXBP PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 8.639e-19
Identity = 61/189 (32.28%), Postives = 81/189 (42.86%), Query Frame = 1

Query:   64 KMSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQ---GSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPN------GPSEKKCYKCQNTGHIARDC 603
            K  +R + C+ CG++GH SREC    R G+      C+ CGE GH+S+DCP                                            + G+ +   CY CGQ GH+S  CP    GSR    + RG      G      CY C   GH+SR+CPN      G  ++ CYKC + GHI+RDC
Sbjct:   37 KGDERSTTCFRCGEEGHMSRECPNEARSGAAGAM-TCFRCGEAGHMSRDCPN-----------------------------------------SAKPGAAKGFECYKCGQEGHLSRDCPSSQGGSRGGYGQKRGRSGAQGGYSGDRTCYKCGDAGHISRDCPNGQGGYSGAGDRTCYKCGDAGHISRDC 183

HSP 2 Score: 80.4925 bits (197), Expect = 6.065e-17
Identity = 70/194 (36.08%), Postives = 87/194 (44.85%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK---RYGSGSKPG------------ACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPN----GPSEKKCYKCQNTGHIARDCTD 609
            ECY CGQ+GH SR+C  S+   R G G K G             CY CG+ GHIS+DCP G+     AGDR                                         CY CG +GHIS  CP G        +G Y  G G+    KCY C ++GHMSRECP+    G  ++ CYKC   GHI+R+C +
Sbjct:   98 ECYKCGQEGHLSRDCPSSQGGSRGGYGQKRGRSGAQGGYSGDRTCYKCGDAGHISRDCPNGQGGYSGAGDR----------------------------------------TCYKCGDAGHISRDCPNG--------QGGYS-GAGD---RKCYKCGESGHMSRECPSAGSTGSGDRACYKCGKPGHISRECPE 239

HSP 3 Score: 55.4546 bits (132), Expect = 6.019e-8
Identity = 47/152 (30.92%), Postives = 63/152 (41.45%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPN 543
            CY CG  GH SR+C   +   SG+    CY CG+ GHIS+DCP G+     AGDR+     +     R+                           CY CG+ GHIS  CP+   +                    CY C + GH+SR+CP+
Sbjct:  142 CYKCGDAGHISRDCPNGQGGYSGAGDRTCYKCGDAGHISRDCPNGQGGYSGAGDRKCYKCGESGHMSRECPSAGSTGSGDR--------------ACYKCGKPGHISRECPEAGGSYGGSR---------GGGDRTCYKCGEAGHISRDCPS 270

HSP 4 Score: 51.9878 bits (123), Expect = 7.484e-7
Identity = 27/81 (33.33%), Postives = 38/81 (46.91%), Query Frame = 1

Query:  376 CYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPN-----GPSEKKCYKCQNTGHIARDC 603
            C +CG+ GH +  CP+     D R+               C+ C + GHMSRECPN           C++C   GH++RDC
Sbjct:   18 CRNCGKEGHYARECPEADSKGDERS-------------TTCFRCGEEGHMSRECPNEARSGAAGAMTCFRCGEAGHMSRDC 85

[Close]

BLAST of SMED30003997 vs. UniProt/SwissProt
Match: sp|Q3T0Q6|CNBP_BOVIN (Cellular nucleic acid-binding protein OS=Bos taurus OX=9913 GN=CNBP PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 7.070e-13
Identity = 51/187 (27.27%), Postives = 74/187 (39.57%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK-------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                 ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 126

HSP 2 Score: 60.077 bits (144), Expect = 4.420e-10
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   47 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 165

[Close]

BLAST of SMED30003997 vs. UniProt/SwissProt
Match: sp|O42395|CNBP_CHICK (Cellular nucleic acid-binding protein OS=Gallus gallus OX=9031 GN=CNBP PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.753e-13
Identity = 51/188 (27.13%), Postives = 75/188 (39.89%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                  ++ S S P  CY CGE+GH++KDC   E +                                                CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRTGHWARECPTGIGRGRGMRSRGRAGFQFMSSSLPDICYRCGESGHLAKDCDLQEDK-----------------------------------------------ACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 128

HSP 2 Score: 63.1586 bits (152), Expect = 3.549e-11
Identity = 49/178 (27.53%), Postives = 69/178 (38.76%), Query Frame = 1

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            Q    CYNCG+ GH +++C E KR     +   CYNCG+ GH+++DC   + +                                                CYSCG+ GHI   C                       +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   64 QEDKACYNCGRGGHIAKDCKEPKR----EREQCCYNCGKPGHLARDCDHADEQK-----------------------------------------------CYSCGEFGHIQKDCT----------------------KVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 167

[Close]

BLAST of SMED30003997 vs. UniProt/SwissProt
Match: sp|P53849|GIS2_YEAST (Zinc finger protein GIS2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GIS2 PE=1 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.305e-12
Identity = 56/205 (27.32%), Postives = 77/205 (37.56%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPG---------------ACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQ-----------VKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDC 603
            MSQ+   CY CG+ GH + +C   +   + +KPG                CYNCGE GH+  +C                                                      C++C Q+GHIS  CP+  + +       YK G  N              +KCY C Q GHMSR+C N   ++ CY C  TGHI++DC
Sbjct:    1 MSQK--ACYVCGKIGHLAEDCDSERLCYNCNKPGHVQTDCTMPRTVEFKQCYNCGETGHVRSECTVQR--------------------------------------------------CFNCNQTGHISRECPEPKKTSRFSKVSCYKCGGPNHMAKDCMKEDGISGLKCYTCGQAGHMSRDCQN---DRLCYNCNETGHISKDC 150

HSP 2 Score: 50.447 bits (119), Expect = 8.369e-7
Identity = 43/153 (28.10%), Postives = 58/153 (37.91%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNG 546
            C+NC Q GH SREC E K+    SK  +CY CG   H++KDC + +                                                  CY+CGQ+GH+S  C                    ND+   CYNCN+ GH+S++CP  
Sbjct:   67 CFNCNQTGHISRECPEPKKTSRFSKV-SCYKCGGPNHMAKDCMKEDGISGLK--------------------------------------------CYTCGQAGHMSRDCQ-------------------NDRL--CYNCNETGHISKDCPKA 153

HSP 3 Score: 50.0618 bits (118), Expect = 1.334e-6
Identity = 30/87 (34.48%), Postives = 43/87 (49.43%), Query Frame = 1

Query:  373 VCYSCGQSGHISSYC--PQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGP-----SEKKCYKCQNTGHIARDCTDD 612
            +CY+C + GH+ + C  P+        N G            +C+NCNQ GH+SRECP        S+  CYKC    H+A+DC  +
Sbjct:   24 LCYNCNKPGHVQTDCTMPRTVEFKQCYNCGETGHVRSECTVQRCFNCNQTGHISRECPEPKKTSRFSKVSCYKCGGPNHMAKDCMKE 110

[Close]

BLAST of SMED30003997 vs. UniProt/SwissProt
Match: sp|P62634|CNBP_RAT (Cellular nucleic acid-binding protein OS=Rattus norvegicus OX=10116 GN=Cnbp PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 6.262e-12
Identity = 51/194 (26.29%), Postives = 74/194 (38.14%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESK--------------------RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            EC+ CG+ GH++REC                        ++ S S P  CY CGE+GH++KDC   E                                                  CY+CG+ GHI+  C +  R                +++  CYNC + GH++R+C +   E+KCY C   GHI +DCT
Sbjct:    5 ECFKCGRSGHWARECPTGGGRGRGMRSRGRGGFTSDRGFQFVSSSLPDICYRCGESGHLAKDCDLQE------------------------------------------------DACYNCGRGGHIAKDCKEPKR----------------EREQCCYNCGKPGHLARDCDHA-DEQKCYSCGEFGHIQKDCT 133

HSP 2 Score: 60.077 bits (144), Expect = 4.406e-10
Identity = 47/173 (27.17%), Postives = 71/173 (41.04%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCT 606
            CY CG+ GH +++C          +  ACYNCG  GHI+KDC + +R   +                                             CY+CG+ GH++  C          + G +     +  +VKCY C + GH++  C    SE  CY+C  +GH+AR+CT
Sbjct:   54 CYRCGESGHLAKDCD--------LQEDACYNCGRGGHIAKDCKEPKREREQ--------------------------------------------CCYNCGKPGHLARDCDHADEQK-CYSCGEFGHIQKDCTKVKCYRCGETGHVAINCSK-TSEVNCYRCGESGHLARECT 172

[Close]

BLAST of SMED30003997 vs. TrEMBL
Match: A0A5D6XYH8 (Uncharacterized protein OS=Pythium brassicum OX=1485010 GN=PybrP1_002851 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.217e-19
Identity = 53/184 (28.80%), Postives = 78/184 (42.39%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESKRYGSGSKPG---ACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCP---QGSRNNDSRNRGSYKFGDGND--KQVKCYNCNQNGHMSRECP--NGPSEKKCYKCQNTGHIARDCT 606
            +C+ CG  GH  R+C E+     G+  G    C+ CG+ GH+ +DCP G                                                   C++CG  GHI   CP   Q  + ++  N G  +    +D  +  KC++C  +GH+ R+CP  NGPS  KCY+C  +GH AR+CT
Sbjct:  599 KCHTCGNTGHLRRDCPEAPAQAEGAFAGNANTCFGCGKTGHLKRDCPTG------------------------------------------------GRACHNCGNVGHIRRDCPEDVQPPKCHNCGNTGHLRRDCPDDARESRKCHSCGNSGHLRRDCPEDNGPSADKCYQCGESGHWARNCT 734

HSP 2 Score: 79.337 bits (194), Expect = 2.050e-13
Identity = 42/150 (28.00%), Postives = 65/150 (43.33%), Query Frame = 1

Query:  172 CYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRN-NDSRNRGSYKFGDGNDKQV-KCYNCNQNGHMSRECPNGPSE-KKCYKCQNTGHIARDCTDD 612
            C+ CG  GH+ +DCP+   +   A    ++                                      C+ CG++GH+   CP G R  ++  N G  +     D Q  KC+NC   GH+ R+CP+   E +KC+ C N+GH+ RDC +D
Sbjct:  600 CHTCGNTGHLRRDCPEAPAQAEGAFAGNAN-------------------------------------TCFGCGKTGHLKRDCPTGGRACHNCGNVGHIRRDCPEDVQPPKCHNCGNTGHLRRDCPDDARESRKCHSCGNSGHLRRDCPED 712

[Close]

BLAST of SMED30003997 vs. TrEMBL
Match: A0A1X0P815 (Universal minicircle sequence binding protein (UMSBP) OS=Trypanosoma theileri OX=67003 GN=TM35_000023440 PE=4 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.149e-17
Identity = 61/180 (33.89%), Postives = 75/180 (41.67%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPN--------GPSEKKCYKCQNTGHIARDC 603
            CYNCGQ GH SREC      G+G +  ACYNCGE GH+S++CP                                        +     G      CY+CG++GH+S  CP  +                      CYNC Q+GHMSRECPN         P  + CY CQ  GH+ARDC
Sbjct:   46 CYNCGQTGHMSRECPTKAPGGAGGR--ACYNCGETGHLSRECPT---------------------------------------KTAGGAGGAGGRACYNCGETGHLSRECPTKTAGGAG-----------GAGGRACYNCGQSGHMSRECPNRGGDGYRGAPRSRACYHCQQEGHLARDC 173

HSP 2 Score: 78.5666 bits (192), Expect = 3.562e-14
Identity = 59/180 (32.78%), Postives = 78/180 (43.33%), Query Frame = 1

Query:   88 CYNCGQDGHYSREC-TESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPS-------EKKCYKCQNTGHIARDC 603
            CYNCG+ GH SREC T++     G+   ACYNCGE GH+S++CP                                        +     G      CY+CGQSGH+S  CP         NRG   +  G  +   CY+C Q GH++R+CP  P+       +  CY C   GHI+R C
Sbjct:   72 CYNCGETGHLSRECPTKTAGGAGGAGGRACYNCGETGHLSRECPT---------------------------------------KTAGGAGGAGGRACYNCGQSGHMSRECP---------NRGGDGY-RGAPRSRACYHCQQEGHLARDCPTAPAGGAPRGGDATCYNCGQPGHISRAC 202

[Close]

BLAST of SMED30003997 vs. TrEMBL
Match: A0A3S5IS77 (Universal minicircle sequence binding protein (UMSBP) OS=Trypanosoma conorhini OX=83891 GN=Tco025E_06913 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.192e-17
Identity = 60/184 (32.61%), Postives = 84/184 (45.65%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPS-----EKKCYKCQNTGHIARDC 603
            M++    CYNCGQ GH SREC      G+G    ACYNCG+ GH+S++CP                                             GG+     CY+CGQ GHIS  CP       +R+ G ++       +  CY+C Q GH++R+CPN P+     ++ CY C  TGH++R+C
Sbjct:    6 MTRGDRACYNCGQPGHLSRECPTRPPGGAGGDR-ACYNCGQTGHLSRECPT-----------------------------------------RLPGGAGGDRACYNCGQPGHISRDCP-------TRSAGGFR----GAVRGACYHCQQEGHLARDCPNAPAGGAGGDRACYNCGQTGHLSREC 136

[Close]

BLAST of SMED30003997 vs. TrEMBL
Match: S2JQ68 (Uncharacterized protein OS=Mucor circinelloides f. circinelloides (strain 1006PhL) OX=1220926 GN=HMPREF1544_08490 PE=4 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.677e-17
Identity = 67/193 (34.72%), Postives = 84/193 (43.52%), Query Frame = 1

Query:   70 SQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFG-DGNDKQVKCYNCNQNGHMSRECP----NGPSEKKCYKCQNTGHIARDCTDD*LIQTCF 633
            SQ  S CYNC Q GH S++CTE++          CY C + GHIS+DCP+    D                                               CYSCG+SGH+S  CP+G       N G Y  G  G      CY+C Q GHMSR+CP     G   +KCY C N+GHI+RDC      +TC+
Sbjct:   23 SQAESVCYNCQQGGHMSKDCTEAR------AEKKCYKCSQTGHISRDCPEASSNDGP--------------------------------------------ACYSCGKSGHMSRNCPEGG------NNGGYDSGRSGYGAPKSCYSCGQTGHMSRDCPSGGSGGYGGQKCYNCGNSGHISRDCDMPAQAKTCY 159

HSP 2 Score: 60.077 bits (144), Expect = 1.577e-7
Identity = 32/79 (40.51%), Postives = 41/79 (51.90%), Query Frame = 1

Query:  373 VCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            +CY CG+ GH +  C Q                     +  CYNC Q GHMS++C    +EKKCYKC  TGHI+RDC +
Sbjct:    8 ICYKCGEPGHQARDCSQA--------------------ESVCYNCQQGGHMSKDCTEARAEKKCYKCSQTGHISRDCPE 66

[Close]

BLAST of SMED30003997 vs. TrEMBL
Match: D3BUZ6 (Uncharacterized protein OS=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) OX=670386 GN=PPL_11968 PE=4 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.945e-17
Identity = 63/176 (35.80%), Postives = 79/176 (44.89%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGA--CYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            C+ CG+ GH SRECT+    G GS  G   C+NCG+ GHIS++CP G       G                                           CY+CG +GHIS  CP  S  ND           G D+   C+NC + GHMSR+C  G S   C+KC  TGHI+RDCT+
Sbjct:   39 CFKCGKPGHMSRECTQ----GGGSDRGGNNCFNCGKPGHISRECPDGAAGAGNTGS------------------------------------------CYNCGNTGHISRECPNKSERNDR---------SGGDRA--CFNCGKTGHMSRDCTQGGSSAGCFKCGKTGHISRDCTE 157

HSP 2 Score: 75.8702 bits (185), Expect = 7.108e-13
Identity = 59/175 (33.71%), Postives = 78/175 (44.57%), Query Frame = 1

Query:   88 CYNCGQDGHYSREC-TESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSE-KKCYKCQNTGHIARDCT 606
            CYNCG  GH SREC  +S+R        AC+NCG+ GH+S+DC QG                                                S+ C+ CG++GHIS  C +          G    G G DK  KC+ CNQ GH+SR+CPN  S+   C+ C  +GH +R+CT
Sbjct:   91 CYNCGNTGHISRECPNKSERNDRSGGDRACFNCGKTGHMSRDCTQG----------------------------------------------GSSAGCFKCGKTGHISRDCTE---------SGGSDRGHGGDK--KCFKCNQTGHISRDCPNSDSQGPSCFNCGESGHKSRECT 208

HSP 3 Score: 58.151 bits (139), Expect = 1.860e-6
Identity = 36/93 (38.71%), Postives = 46/93 (49.46%), Query Frame = 1

Query:  376 CYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPS-------EKKCYKCQNTGHIARDCTDD*LIQTCF 633
            C++CG+ GHIS  CP G+             G GN     CYNC   GH+SRECPN          ++ C+ C  TGH++RDCT       CF
Sbjct:   65 CFNCGKPGHISRECPDGA------------AGAGN--TGSCYNCGNTGHISRECPNKSERNDRSGGDRACFNCGKTGHMSRDCTQGGSSAGCF 143

[Close]

BLAST of SMED30003997 vs. Ensembl Cavefish
Match: cnbpa (cellular nucleic acid-binding protein-like [Source:NCBI gene;Acc:103024401])

HSP 1 Score: 63.929 bits (154), Expect = 2.118e-12
Identity = 55/186 (29.57%), Postives = 86/186 (46.24%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGS----KPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            M    SEC+ CG+ GH+ + C  + R         K   CY CGE GHI++DC Q E  +  +   ++ +   R      D ++ +++  +                CY+CG++GH++  C   +        G        DK VKCY C + GH++ +C    SE  CY C  TGH+AR+CT +
Sbjct:    1 MDMSSSECFGCGRSGHWVKNCPNAGRGRGRGRGRGKDLFCYRCGEQGHIARDCEQTEDGEALSPFSQTCYNCHRSGHISRDCKEPKKEREQ---------------CCYNCGKAGHVARDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVQCSKA-SEVNCYNCGKTGHLARECTIE 169

[Close]

BLAST of SMED30003997 vs. Ensembl Cavefish
Match: ENSAMXT00000005562.2 (cellular nucleic acid-binding protein [Source:NCBI gene;Acc:103027392])

HSP 1 Score: 63.1586 bits (152), Expect = 2.963e-12
Identity = 49/175 (28.00%), Postives = 75/175 (42.86%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            CY CG+ GH +R+C  ++         ACYNCG  GHIS+DC + ++   +                                            VCY+CG++GH++  C   +        G      G +K VKCY C + GH++ +C    SE  CY C  +GH+A++CT +
Sbjct:   38 CYRCGEPGHVARDCERTE--------DACYNCGRGGHISRDCKEPKKEREQ--------------------------------------------VCYNCGKAGHVARDCDHANEQKCYSCGGFGHIQKGCEK-VKCYRCGEIGHVAVQCSK-ASEVNCYNCGKSGHLAKECTIE 158

HSP 2 Score: 51.6026 bits (122), Expect = 4.446e-8
Identity = 44/177 (24.86%), Postives = 69/177 (38.98%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESKRYGSGSKPGA----CYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDC 603
            EC+ CG+ GH+ + C  + R     +       CY CGE GH+++DC + E                                                  CY+CG+ GHIS  C +  +                +++  CYNC + GH++R+C +  +E+KCY C   GHI + C
Sbjct:    5 ECFGCGRTGHWIKNCPNAGRGRGKGRGRGKDLFCYRCGEPGHVARDCERTED------------------------------------------------ACYNCGRGGHISRDCKEPKK----------------EREQVCYNCGKAGHVARDCDH-ANEQKCYSCGGFGHIQKGC 116

[Close]

BLAST of SMED30003997 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003739.1 (pep scaffold:Pmarinus_7.0:GL499243:411:1384:-1 gene:ENSPMAG00000003418.1 transcript:ENSPMAT00000003739.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.1586 bits (152), Expect = 3.781e-13
Identity = 50/187 (26.74%), Postives = 70/187 (37.43%), Query Frame = 1

Query:   85 ECYNCGQDGHYSREC----------TESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNG---PSEKKCYKCQNTGHIARDCT 606
            EC+ CG  GH++REC                G   +   CY CGE GHI+++CP  +                                                  CY+CG+ GHI+  CP+G ++                    CY C + GH++REC +G   P + KCY C   GH+ RDCT
Sbjct:    5 ECFRCGGSGHWARECPNGAGGGRGPGGPVGRGGRGRGDGCYRCGEGGHIARECPLPQD------------------------------------------------TCYNCGKGGHIARECPEGRQDRGG--------------GPSCYTCGKQGHLARECSSGGGGPGDNKCYGCGQRGHMQRDCT 129

[Close]

BLAST of SMED30003997 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010393.1 (pep scaffold:Pmarinus_7.0:GL485791:8073:10868:-1 gene:ENSPMAG00000009411.1 transcript:ENSPMAT00000010393.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 2.068e-11
Identity = 40/149 (26.85%), Postives = 56/149 (37.58%), Query Frame = 1

Query:  169 ACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNG---PSEKKCYKCQNTGHIARDCT 606
             CY CGE GHI+++CP  +                                                  CY+CG+ GHI+  CP+G ++                    CY C + GH++REC +G   P + KCY C   GH+ RDCT
Sbjct:    9 GCYRCGEGGHIARECPLPQDSVSSNTA-----------------------------------------ACYNCGKGGHIARECPEGRQDRGG--------------GPSCYTCGKQGHLARECSSGGGGPGDNKCYGCGQRGHMQRDCT 102

HSP 2 Score: 53.1434 bits (126), Expect = 9.458e-10
Identity = 42/150 (28.00%), Postives = 56/150 (37.33%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSREC 537
            CY CG+ GH +REC   +   S S   ACYNCG+ GHI+++CP+G +                                                 CY+CG+ GH++  C  G                G     KCY C Q GHM R+C
Sbjct:   10 CYRCGEGGHIARECPLPQDSVS-SNTAACYNCGKGGHIARECPEGRQDRGGGPS------------------------------------------CYTCGKQGHLARECSSGG---------------GGPGDNKCYGCGQRGHMQRDC 101

HSP 3 Score: 44.2838 bits (103), Expect = 1.403e-6
Identity = 23/52 (44.23%), Postives = 33/52 (63.46%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQG 222
            +S   + CYNCG+ GH +REC E  R   G  P +CY CG+ GH++++C  G
Sbjct:   30 VSSNTAACYNCGKGGHIARECPEG-RQDRGGGP-SCYTCGKQGHLARECSSG 79

[Close]

BLAST of SMED30003997 vs. Ensembl Yeast
Match: GIS2 (Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 [Source:SGD;Acc:S000005199])

HSP 1 Score: 65.4698 bits (158), Expect = 4.759e-14
Identity = 56/205 (27.32%), Postives = 77/205 (37.56%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPG---------------ACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQ-----------VKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDC 603
            MSQ+   CY CG+ GH + +C   +   + +KPG                CYNCGE GH+  +C                                                      C++C Q+GHIS  CP+  + +       YK G  N              +KCY C Q GHMSR+C N   ++ CY C  TGHI++DC
Sbjct:    1 MSQK--ACYVCGKIGHLAEDCDSERLCYNCNKPGHVQTDCTMPRTVEFKQCYNCGETGHVRSECTVQR--------------------------------------------------CFNCNQTGHISRECPEPKKTSRFSKVSCYKCGGPNHMAKDCMKEDGISGLKCYTCGQAGHMSRDCQN---DRLCYNCNETGHISKDC 150

HSP 2 Score: 50.447 bits (119), Expect = 1.205e-8
Identity = 43/153 (28.10%), Postives = 58/153 (37.91%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNG 546
            C+NC Q GH SREC E K+    SK  +CY CG   H++KDC + +                                                  CY+CGQ+GH+S  C                    ND+   CYNCN+ GH+S++CP  
Sbjct:   67 CFNCNQTGHISRECPEPKKTSRFSKV-SCYKCGGPNHMAKDCMKEDGISGLK--------------------------------------------CYTCGQAGHMSRDCQ-------------------NDRL--CYNCNETGHISKDCPKA 153

HSP 3 Score: 50.0618 bits (118), Expect = 1.921e-8
Identity = 30/87 (34.48%), Postives = 43/87 (49.43%), Query Frame = 1

Query:  373 VCYSCGQSGHISSYC--PQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGP-----SEKKCYKCQNTGHIARDCTDD 612
            +CY+C + GH+ + C  P+        N G            +C+NCNQ GH+SRECP        S+  CYKC    H+A+DC  +
Sbjct:   24 LCYNCNKPGHVQTDCTMPRTVEFKQCYNCGETGHVRSECTVQRCFNCNQTGHISRECPEPKKTSRFSKVSCYKCGGPNHMAKDCMKE 110

HSP 4 Score: 46.9802 bits (110), Expect = 2.470e-7
Identity = 30/90 (33.33%), Postives = 41/90 (45.56%), Query Frame = 1

Query:  373 VCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSREC--PNGPSEKKCYKCQNTGHIARDCTDD*LIQTCFN 636
             CY CG+ GH++  C                     D +  CYNCN+ GH+  +C  P     K+CY C  TGH+  +CT    +Q CFN
Sbjct:    5 ACYVCGKIGHLAEDC---------------------DSERLCYNCNKPGHVQTDCTMPRTVEFKQCYNCGETGHVRSECT----VQRCFN 69

HSP 5 Score: 46.2098 bits (108), Expect = 4.152e-7
Identity = 23/46 (50.00%), Postives = 27/46 (58.70%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQG 222
            +CY CGQ GH SR+C   +          CYNC E GHISKDCP+ 
Sbjct:  117 KCYTCGQAGHMSRDCQNDR---------LCYNCNETGHISKDCPKA 153

[Close]

BLAST of SMED30003997 vs. Ensembl Nematostella
Match: EDO27708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T8H9])

HSP 1 Score: 63.5438 bits (153), Expect = 6.374e-12
Identity = 47/187 (25.13%), Postives = 68/187 (36.36%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGP------------SEKKCYKCQNTGHIARDCTDD 612
            C  CG+ GH++R+C +    G G     C+ C E GH +++CP  +    +                                             C+ C +SGH +  CP           GS            CY CN+ GH +RECPN              S+  C+KCQ TGH AR+C ++
Sbjct:   36 CRKCGESGHFARDCPQ----GGGGGSRTCHKCNEEGHFARECPNADSGGNK---------------------------------------------CFKCNESGHFARECPNSGGGGGGFGGGS--------SGSTCYKCNETGHFARECPNAESNGGGFGGGGGSSDSTCFKCQQTGHFARECPNE 165

[Close]

BLAST of SMED30003997 vs. Ensembl Nematostella
Match: EDO36176 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJG4])

HSP 1 Score: 46.9802 bits (110), Expect = 5.283e-7
Identity = 30/81 (37.04%), Postives = 42/81 (51.85%), Query Frame = 1

Query:  373 VCYSCGQSGHISSYCP-QGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKK---CYKCQNTGHIARDC 603
             C+ CG+ GH S  CP Q S+  + +      F     +   C+ C Q GH SRECPN   + +   C+KC  TGH +R+C
Sbjct:   53 ACHKCGKEGHFSRECPNQDSQRMNIQYLCQTHFSISGGR--NCHKCGQEGHFSRECPNQAIQGQSDTCHKCGETGHYSREC 131

HSP 2 Score: 46.9802 bits (110), Expect = 5.949e-7
Identity = 43/160 (26.88%), Postives = 57/160 (35.62%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTES-------KRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPN 543
            +C+ CG+ GH+SREC          KR G G   GAC+ CG+ GH S++CP  + +          H                                     C+ CGQ GH S  CP  +    S                 C+ C + GH SRECP 
Sbjct:   21 DCHQCGEAGHFSRECPNKGNQGEPIKRMGGG---GACHKCGKEGHFSRECPNQDSQRMNIQYLCQTHFSISGGRN-----------------------------CHKCGQEGHFSRECPNQAIQGQSDT---------------CHKCGETGHYSRECPT 133

[Close]

BLAST of SMED30003997 vs. Ensembl Medaka
Match: cnbpa (CCHC-type zinc finger nucleic acid binding protein [Source:NCBI gene;Acc:101173222])

HSP 1 Score: 58.5362 bits (140), Expect = 1.357e-10
Identity = 47/175 (26.86%), Postives = 72/175 (41.14%), Query Frame = 1

Query:   88 CYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            CY CG  GH +R+C +++         ACYNC  +GHIS+DC + ++   +                                            +CY+CG++GH++  C   +        G        DK VKCY C + GH++  C    SE  CY C   GH+A++CT +
Sbjct:   43 CYRCGDQGHMARDCDQTE--------DACYNCHRSGHISRDCKEPKKEREQ--------------------------------------------LCYTCGKAGHMARDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVHCSKA-SETNCYNCGKAGHLAKECTIE 163

HSP 2 Score: 53.5286 bits (127), Expect = 1.012e-8
Identity = 48/187 (25.67%), Postives = 73/187 (39.04%), Query Frame = 1

Query:   61 LKMSQRRSECYNCGQDGHYSRECTES------KRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDC 603
            ++MS   SEC+ CG+ GH+ + C  +       R     K   CY CG+ GH+++DC Q E                                                  CY+C +SGHIS  C +  +                +++  CY C + GHM+R+C +  +E+KCY C   GHI + C
Sbjct:    1 MEMSSN-SECFGCGRPGHWVKHCPNASGTRGRGRGRGRGKELFCYRCGDQGHMARDCDQTED------------------------------------------------ACYNCHRSGHISRDCKEPKK----------------EREQLCYTCGKAGHMARDCDHA-NEQKCYSCGGFGHIQKLC 121

HSP 3 Score: 50.8322 bits (120), Expect = 8.707e-8
Identity = 27/78 (34.62%), Postives = 38/78 (48.72%), Query Frame = 1

Query:  376 CYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            C+ CG+ GH   +CP  S                  K++ CY C   GHM+R+C    +E  CY C  +GHI+RDC +
Sbjct:    9 CFGCGRPGHWVKHCPNASGTRGRGRGRG------RGKELFCYRCGDQGHMARDCDQ--TEDACYNCHRSGHISRDCKE 78

[Close]

BLAST of SMED30003997 vs. Ensembl Medaka
Match: cnbpa (CCHC-type zinc finger nucleic acid binding protein [Source:NCBI gene;Acc:101173222])

HSP 1 Score: 57.3806 bits (137), Expect = 2.999e-10
Identity = 50/188 (26.60%), Postives = 78/188 (41.49%), Query Frame = 1

Query:   61 LKMSQRRSECYNCGQDGHYSRECTESK----RYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            ++MS   SEC+ CG+ GH+ + C  +     R     +  ACYNC  +GHIS+DC + ++   +                                            +CY+CG++GH++  C   +        G        DK VKCY C + GH++  C    SE  CY C   GH+A++CT +
Sbjct:    1 MEMSSN-SECFGCGRPGHWVKHCPNASGTRGRGRGRGRGKACYNCHRSGHISRDCKEPKKEREQ--------------------------------------------LCYTCGKAGHMARDCDHANEQKCYSCGGFGHIQKLCDK-VKCYRCGEIGHVAVHCSKA-SETNCYNCGKAGHLAKECTIE 141

[Close]

BLAST of SMED30003997 vs. Ensembl Medaka
Match: ENSORLT00000043369.1 (pep primary_assembly:ASM223467v1:17:15543647:15547570:1 gene:ENSORLG00000023375.1 transcript:ENSORLT00000043369.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.1358 bits (113), Expect = 3.744e-6
Identity = 20/47 (42.55%), Postives = 27/47 (57.45%), Query Frame = 1

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDC 213
            + R +C+ CG+ GH SRECT +K          C NCG+ GH +K C
Sbjct:  212 EHRIKCFRCGRSGHLSRECTITKN-------KKCNNCGKEGHFAKMC 251

HSP 2 Score: 47.3654 bits (111), Expect = 6.206e-6
Identity = 18/44 (40.91%), Postives = 27/44 (61.36%), Query Frame = 1

Query:  472 DGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDC 603
            +G + ++KC+ C ++GH+SREC      KKC  C   GH A+ C
Sbjct:  209 EGGEHRIKCFRCGRSGHLSREC-TITKNKKCNNCGKEGHFAKMC 251

[Close]

BLAST of SMED30003997 vs. Planmine SMEST
Match: SMESG000059318.1 (SMESG000059318.1)

HSP 1 Score: 249.595 bits (636), Expect = 1.447e-84
Identity = 177/194 (91.24%), Postives = 178/194 (91.75%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------------XXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGERRDRRAGDRRSDHRDDRRDDRRDDRRDDRRDDRR+  RDDRR              SQRSSVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD
Sbjct:    1 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGERRDRRAGDRRSDHRDDRRDDRRDDRRDDRRDDRRDDRRDDRRDDRREHPRDDRRGGSQRSSVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 194

[Close]

BLAST of SMED30003997 vs. Planmine SMEST
Match: SMESG000059304.1 (SMESG000059304.1)

HSP 1 Score: 249.595 bits (636), Expect = 1.447e-84
Identity = 177/194 (91.24%), Postives = 178/194 (91.75%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------------XXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGERRDRRAGDRRSDHRDDRRDDRRDDRRDDRRDDRR+  RDDRR              SQRSSVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD
Sbjct:    1 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGERRDRRAGDRRSDHRDDRRDDRRDDRRDDRRDDRRDDRRDDRRDDRREHPRDDRRGGSQRSSVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 194

[Close]

BLAST of SMED30003997 vs. Planmine SMEST
Match: SMESG000059283.1 (SMESG000059283.1)

HSP 1 Score: 137.887 bits (346), Expect = 5.246e-41
Identity = 90/196 (45.92%), Postives = 114/196 (58.16%), Query Frame = 1

Query:   67 MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDG---------------NDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            MS RR ECYNCG+DGH SR+CTESK+ GSG + GAC+NCGE+GH+S++CPQ   R            DDRR ++R                            CY+C +SGH+S  CP+  R+N  R+ G YK G+                 ++QVKCYNCNQNGHMSRECPNG SEKKCYKC   GH++R+C +
Sbjct:    1 MSSRRMECYNCGKDGHMSRDCTESKKSGSGPRSGACFNCGESGHMSRECPQNGSRGGDRRGGYERRGDDRRGNQRGTE-------------------------CYNCHESGHMSRECPK-QRSNGPRSNGCYKCGESGHMARDCNQEGSDNRQERQVKCYNCNQNGHMSRECPNGQSEKKCYKCNKIGHMSRECNE 170

HSP 2 Score: 72.7886 bits (177), Expect = 7.847e-16
Identity = 52/159 (32.70%), Postives = 73/159 (45.91%), Query Frame = 1

Query:  157 SKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGD---GNDKQVKCYNCNQNGHMSRECP----NGPSEKKCYKCQNTGHIARDCTDD 612
            S+   CYNCG++GH+S+DC + ++                                                 C++CG+SGH+S  CPQ       R  G  + GD   GN +  +CYNC+++GHMSRECP    NGP    CYKC  +GH+ARDC  +
Sbjct:    3 SRRMECYNCGKDGHMSRDCTESKKSGSGPRSG----------------------------------------ACFNCGESGHMSRECPQNGSRGGDRRGGYERRGDDRRGNQRGTECYNCHESGHMSRECPKQRSNGPRSNGCYKCGESGHMARDCNQE 121

[Close]

BLAST of SMED30003997 vs. Planmine SMEST
Match: SMESG000059283.1 (SMESG000059283.1)

HSP 1 Score: 130.568 bits (327), Expect = 3.827e-38
Identity = 86/190 (45.26%), Postives = 110/190 (57.89%), Query Frame = 1

Query:   85 ECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDG---------------NDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTD 609
            ECYNCG+DGH SR+CTESK+ GSG + GAC+NCGE+GH+S++CPQ   R            DDRR ++R                            CY+C +SGH+S  CP+  R+N  R+ G YK G+                 ++QVKCYNCNQNGHMSRECPNG SEKKCYKC   GH++R+C +
Sbjct:    2 ECYNCGKDGHMSRDCTESKKSGSGPRSGACFNCGESGHMSRECPQNGSRGGDRRGGYERRGDDRRGNQRGTE-------------------------CYNCHESGHMSRECPK-QRSNGPRSNGCYKCGESGHMARDCNQEGSDNRQERQVKCYNCNQNGHMSRECPNGQSEKKCYKCNKIGHMSRECNE 165

HSP 2 Score: 71.633 bits (174), Expect = 1.779e-15
Identity = 51/154 (33.12%), Postives = 71/154 (46.10%), Query Frame = 1

Query:  172 CYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGD---GNDKQVKCYNCNQNGHMSRECP----NGPSEKKCYKCQNTGHIARDCTDD 612
            CYNCG++GH+S+DC + ++                                                 C++CG+SGH+S  CPQ       R  G  + GD   GN +  +CYNC+++GHMSRECP    NGP    CYKC  +GH+ARDC  +
Sbjct:    3 CYNCGKDGHMSRDCTESKKSGSGPRSG----------------------------------------ACFNCGESGHMSRECPQNGSRGGDRRGGYERRGDDRRGNQRGTECYNCHESGHMSRECPKQRSNGPRSNGCYKCGESGHMARDCNQE 116

HSP 3 Score: 59.6918 bits (143), Expect = 4.616e-11
Identity = 48/155 (30.97%), Postives = 64/155 (41.29%), Query Frame = 1

Query:   73 QRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQNGHMSREC 537
            QR +ECYNC + GH SREC   K+  +G +   CY CGE+GH+++DC Q                                        +      +R   CY+C Q+GH+S  CP G                    + KCY CN+ GHMSREC
Sbjct:   69 QRGTECYNCHESGHMSRECP--KQRSNGPRSNGCYKCGESGHMARDCNQ----------------------------------------EGSDNRQERQVKCYNCNQNGHMSRECPNG------------------QSEKKCYKCNKIGHMSREC 163

[Close]

BLAST of SMED30003997 vs. Planmine SMEST
Match: SMESG000059266.1 (SMESG000059266.1)

HSP 1 Score: 119.013 bits (297), Expect = 2.228e-33
Identity = 85/228 (37.28%), Postives = 104/228 (45.61%), Query Frame = 1

Query:   61 LKMSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGAC--------------------------------YNCGENGHISKDCPQGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGD------------GNDKQVKCYNCNQNGHMSRECPNGPSEKKCYKCQNTGHIARDCTDD 612
            +  +QR+ +CYNCG+DGH +R+CTE K+ GS  +  AC                                YNCGE GH+SKDC                                             R  + R + CY CG++GHIS  C Q    +  R    YK               GND+Q+KCYNC+QNGHMSRECPNGPSEKKCYKC N GH+ARDCTDD
Sbjct:    1 MSYNQRKLDCYNCGKDGHMARDCTEYKKSGSNQRTNACFNCGLNGHISKECPDNRRGGGNRRGGRSSVTCYNCGEAGHVSKDCSD----------------------------------------QGYRDNAPRGNTCYKCGEAGHISRNCTQNWSGD--RQMKCYKCNQLGHMSRDCNEDGGNDRQIKCYNCHQNGHMSRECPNGPSEKKCYKCNNPGHLARDCTDD 186

The following BLAST results are available for this feature:

BLAST of SMED30003997 vs. Ensembl Human
BLAST of SMED30003997 vs. Ensembl Celegans
BLAST of SMED30003997 vs. Ensembl Fly
BLAST of SMED30003997 vs. Ensembl Zebrafish
BLAST of SMED30003997 vs. Ensembl Xenopus
BLAST of SMED30003997 vs. Ensembl Mouse
BLAST of SMED30003997 vs. UniProt/SwissProt
BLAST of SMED30003997 vs. TrEMBL
BLAST of SMED30003997 vs. Ensembl Cavefish
BLAST of SMED30003997 vs. Ensembl Sea Lamprey
BLAST of SMED30003997 vs. Ensembl Yeast
BLAST of SMED30003997 vs. Ensembl Nematostella
BLAST of SMED30003997 vs. Ensembl Medaka
BLAST of SMED30003997 vs. Planmine SMEST

BLAST of SMED30003997 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CNBP	1.353e-13	27.27	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	1.472e-13	27.27	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	1.148e-12	26.29	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	1.304e-12	26.29	CCHC-type zinc finger nucleic acid binding protein...	[more]
CNBP	1.369e-12	26.29	CCHC-type zinc finger nucleic acid binding protein...	[more]

BLAST of SMED30003997 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
K08D12.3	1.219e-8	45.07	pep chromosome:WBcel235:IV:1710265:1711127:1 gene:...	[more]

BLAST of SMED30003997 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
CNBP	4.799e-11	26.52	gene:FBgn0034802 transcript:FBtr0303464	[more]
CNBP	4.799e-11	26.52	gene:FBgn0034802 transcript:FBtr0071994	[more]

BLAST of SMED30003997 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cnbpb	4.560e-12	28.00	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpb	4.560e-12	28.00	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpa	1.499e-11	27.53	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpa	1.499e-11	27.53	CCHC-type zinc finger, nucleic acid binding protei...	[more]
cnbpa	1.499e-11	27.53	CCHC-type zinc finger, nucleic acid binding protei...	[more]

BLAST of SMED30003997 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30003997 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Cnbp	1.011e-13	27.27	cellular nucleic acid binding protein [Source:MGI ...	[more]
Cnbp	1.653e-13	27.13	cellular nucleic acid binding protein [Source:MGI ...	[more]
Cnbp	8.952e-13	26.29	cellular nucleic acid binding protein [Source:MGI ...	[more]
Cnbp	8.952e-13	26.29	cellular nucleic acid binding protein [Source:MGI ...	[more]
Cnbp	9.399e-13	26.29	cellular nucleic acid binding protein [Source:MGI ...	[more]

BLAST of SMED30003997 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q04832\|HEXP_LEIMA	8.639e-19	32.28	DNA-binding protein HEXBP OS=Leishmania major OX=5...	[more]
sp\|Q3T0Q6\|CNBP_BOVIN	7.070e-13	27.27	Cellular nucleic acid-binding protein OS=Bos tauru...	[more]
sp\|O42395\|CNBP_CHICK	8.753e-13	27.13	Cellular nucleic acid-binding protein OS=Gallus ga...	[more]
sp\|P53849\|GIS2_YEAST	3.305e-12	27.32	Zinc finger protein GIS2 OS=Saccharomyces cerevisi...	[more]
sp\|P62634\|CNBP_RAT	6.262e-12	26.29	Cellular nucleic acid-binding protein OS=Rattus no...	[more]

BLAST of SMED30003997 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A5D6XYH8	1.217e-19	28.80	Uncharacterized protein OS=Pythium brassicum OX=14...	[more]
A0A1X0P815	1.149e-17	33.89	Universal minicircle sequence binding protein (UMS...	[more]
A0A3S5IS77	1.192e-17	32.61	Universal minicircle sequence binding protein (UMS...	[more]
S2JQ68	2.677e-17	34.72	Uncharacterized protein OS=Mucor circinelloides f....	[more]
D3BUZ6	4.945e-17	35.80	Uncharacterized protein OS=Polysphondylium pallidu...	[more]

BLAST of SMED30003997 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
cnbpa	2.118e-12	29.57	cellular nucleic acid-binding protein-like [Source...	[more]
ENSAMXT00000005562.2	2.963e-12	28.00	cellular nucleic acid-binding protein [Source:NCBI...	[more]

BLAST of SMED30003997 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000003739.1	3.781e-13	26.74	pep scaffold:Pmarinus_7.0:GL499243:411:1384:-1 gen...	[more]
ENSPMAT00000010393.1	2.068e-11	26.85	pep scaffold:Pmarinus_7.0:GL485791:8073:10868:-1 g...	[more]

BLAST of SMED30003997 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
GIS2	4.759e-14	27.32	Translational activator for mRNAs with internal ri...	[more]

BLAST of SMED30003997 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO27708	6.374e-12	25.13	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO36176	5.283e-7	37.04	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30003997 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
cnbpa	1.357e-10	26.86	CCHC-type zinc finger nucleic acid binding protein...	[more]
cnbpa	2.999e-10	26.60	CCHC-type zinc finger nucleic acid binding protein...	[more]
ENSORLT00000043369.1	3.744e-6	42.55	pep primary_assembly:ASM223467v1:17:15543647:15547...	[more]

BLAST of SMED30003997 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000059318.1	1.447e-84	91.24	SMESG000059318.1	[more]
SMESG000059304.1	1.447e-84	91.24	SMESG000059304.1	[more]
SMESG000059283.1	5.246e-41	45.92	SMESG000059283.1	[more]
SMESG000059283.1	3.827e-38	45.26	SMESG000059283.1	[more]
SMESG000059266.1	2.228e-33	37.28	SMESG000059266.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30003997 ID=SMED30003997|Name=SMED30003997|organism=Schmidtea mediterranea sexual|type=transcript|length=669bp
GTTTAAAATTTCGAACATAAATCACATAATTAAATAATTTTCTGAGTTAT
ATAATTGTGTTTAAAAATGAGTCAACGGAGGTCGGAGTGTTACAATTGTG
GCCAAGATGGTCATTATTCACGAGAGTGTACTGAAAGTAAACGTTACGGA
TCTGGATCAAAGCCTGGTGCATGCTATAATTGTGGAGAAAATGGCCATAT
TTCTAAAGATTGTCCACAAGGTGAAAGAAGAGATCGCAGAGCAGGTGATC
GTAGAAGTGACCATAGAGATGACCGTAGAGATGACCGTAGAGACGATCGT
AGAGATGATCGTAGAGATGATAGAAGAGAACACCCTAGAGACGATAGAAG
AGGAGGATCTCAGAGAAGCAGTGTTTGTTACAGTTGTGGTCAATCCGGTC
ATATTTCAAGTTATTGTCCACAAGGTTCGAGAAATAATGATTCAAGAAAT
CGTGGTTCTTATAAGTTCGGAGATGGTAATGACAAACAAGTAAAATGTTA
CAATTGCAACCAAAATGGCCATATGTCAAGAGAATGCCCTAATGGGCCAT
CGGAAAAAAAATGCTATAAGTGCCAAAATACTGGGCATATTGCAAGAGAT
TGCACTGATGATTGACTGATCCAAACGTGTTTTAATTAGTTGAATAAATG
GAATGTTATTTAAAAAAAA

protein sequence of SMED30003997-orf-1

>SMED30003997-orf-1 ID=SMED30003997-orf-1|Name=SMED30003997-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=183bp
MSQRRSECYNCGQDGHYSRECTESKRYGSGSKPGACYNCGENGHISKDCP
QGERRDRRAGDRRSDHRDDRRDDRRDDRRDDRRDDRREHPRDDRRGGSQR
SSVCYSCGQSGHISSYCPQGSRNNDSRNRGSYKFGDGNDKQVKCYNCNQN
GHMSRECPNGPSEKKCYKCQNTGHIARDCTDD*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000099	neuron
PLANA:0000429	neoblast

Vocabulary: **INTERPRO**
Term	Definition
IPR036875	Znf_CCHC_sf
IPR001878	Znf_CCHC

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0008270	zinc ion binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001878	Zinc finger, CCHC-type	SMART	SM00343	c2hcfinal6	coord: 177..193 e-value: 3.2E-4 score: 30.0 coord: 115..131 e-value: 7.3E-4 score: 28.8 coord: 35..51 e-value: 8.9E-6 score: 35.2 coord: 155..171 e-value: 2.1E-5 score: 34.0 coord: 7..23 e-value: 7.5E-5 score: 32.1
IPR001878	Zinc finger, CCHC-type	PFAM	PF00098	zf-CCHC	coord: 155..171 e-value: 4.5E-8 score: 32.8 coord: 176..192 e-value: 1.3E-7 score: 31.4 coord: 7..23 e-value: 2.2E-7 score: 30.6 coord: 115..131 e-value: 1.6E-6 score: 27.9 coord: 35..51 e-value: 1.7E-8 score: 34.2
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 8..23 score: 11.037
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 36..51 score: 11.598
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 177..193 score: 10.065
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 116..131 score: 10.296
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 155..171 score: 10.905
None	No IPR available	GENE3D	G3DSA:4.10.60.10		coord: 2..30 e-value: 4.9E-7 score: 31.3 coord: 31..59 e-value: 4.5E-8 score: 34.6 coord: 105..141 e-value: 1.5E-6 score: 29.8
None	No IPR available	GENE3D	G3DSA:4.10.60.10		coord: 147..194 e-value: 1.4E-17 score: 65.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 47..121
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 127..143
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 47..110
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 127..146
None	No IPR available	PANTHER	PTHR23002	ZINC FINGER CCHC DOMAIN CONTAINING PROTEIN	coord: 98..192 coord: 4..55
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	SSF57756	Retrovirus zinc finger-like domains	coord: 97..135
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	SSF57756	Retrovirus zinc finger-like domains	coord: 5..52
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	SSF57756	Retrovirus zinc finger-like domains	coord: 148..193

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SMED30003997

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 9

Owlery

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