Granulin b
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003962 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 19Note: Hover over icons to view figure legend
Homology
BLAST of Granulin b vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 143.665 bits (361), Expect = 5.276e-38 Identity = 101/327 (30.89%), Postives = 138/327 (42.20%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRS 1205
+CP+ R++C + TCC+ Y CC NA CC D HCCP+ + CD Q KC+ + ++ +PA V D V EV CP+ T C+ + CCP AVCC D CCP TCD + C PH + M + H S + + SD C +T+G G C CCSD + CCPQ C + + EK + + D T C +TCC S+ CC+L +AVCC DR CCP C++K SC + + + P TF RS
Sbjct: 21 MCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD----------VKCDMEVSCPDGYTCCRLQS-------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVP--CDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQ---PATFLARS 325 HSP 2 Score: 80.1073 bits (196), Expect = 1.061e-15 Identity = 67/237 (28.27%), Postives = 97/237 (40.93%), Query Frame = 3
Query: 213 TVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLL-LGFKQKAS--KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC 914
TVG++ + C + TCC+ + ++ CC + AVCC D HCCP G TCD ++ C + M K L + P L+ V + CP++ T C+ T + CCP AVCCSD CCP+ TC + +C + E++ K + +G Q S CP G C+ CC D + CCP C+ K C
Sbjct: 92 TVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQC--------QRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSC 309 HSP 3 Score: 58.151 bits (139), Expect = 1.311e-8 Identity = 56/216 (25.93%), Postives = 75/216 (34.72%), Query Frame = 3
Query: 588 NAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN---STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC------VETNSNKRRHFPLTFPKRSK 1208
++V C D CP+ +TC C + +G+ G C TCCSD CCPQ T CD +KC +TD T+ H+ + V C D E C TCC + ++GCC AVCC D + CCP CD + +C V H L P+ K
Sbjct: 18 SSVMCPDARSRCPDGSTC----------------------------------------------------CELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHT-----VGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALK 175 HSP 4 Score: 56.6102 bits (135), Expect = 4.880e-8 Identity = 27/66 (40.91%), Postives = 38/66 (57.58%), Query Frame = 3
Query: 966 TNRL----STVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
TNR S+V+CPD + C TCC + YGCC + NA CC+D + CCP + CD+ + C+
Sbjct: 10 TNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCL 75
BLAST of Granulin b vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 144.05 bits (362), Expect = 7.296e-37 Identity = 101/327 (30.89%), Postives = 138/327 (42.20%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRS 1205
+CP+ R++C + TCC+ Y CC NA CC D HCCP+ + CD Q KC+ + ++ +PA V D V EV CP+ T C+ + CCP AVCC D CCP TCD + C PH + M + H S + + SD C +T+G G C CCSD + CCPQ C + + EK + + D T C +TCC S+ CC+L +AVCC DR CCP C++K SC + + + P TF RS
Sbjct: 207 MCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD----------VKCDMEVSCPDGYTCCRLQS-------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVP--CDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQ---PATFLARS 511 HSP 2 Score: 128.642 bits (322), Expect = 1.697e-31 Identity = 98/317 (30.91%), Postives = 138/317 (43.53%), Query Frame = 3
Query: 201 SNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL-IHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHS----PTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
S +VG I CP+ + +C + TCC S+ CC A CC D+ HCCP G+ CD +C+ + K +PA + V VMCP+ +++C D TCC Y CCP NA CCSD CCP+ T CD+ KC S ++ ++ +L H + + + S D Y C + +G G C F+ CC D CCP CD + C + + P L V DN + C S +TCC ++ +GCC + AVCC+D CCP C
Sbjct: 116 SGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT-PTGTHPLAKKLPAQRTNR---------AVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDM------------EVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC 410 HSP 3 Score: 103.99 bits (258), Expect = 3.137e-23 Identity = 90/336 (26.79%), Postives = 131/336 (38.99%), Query Frame = 3
Query: 213 TVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLL-LGFKQKAS--KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
TVG++ + C + TCC+ + ++ CC + AVCC D HCCP G TCD ++ C + M K L + P L+ V + CP++ T C+ T + CCP AVCCSD CCP+ TC + +C + E++ K + +G Q S CP G C+ CC D + CCP C+ K C + V C + C +TCC + + + CC VCCADR CCP +C + C+ + R PL P +
Sbjct: 278 TVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQC--------QRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHF-CHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREA-PRWDAPLRDPALRQL 592 HSP 4 Score: 84.7297 bits (208), Expect = 5.651e-17 Identity = 55/199 (27.64%), Postives = 81/199 (40.70%), Query Frame = 3
Query: 573 CCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRL----STVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
CCP AV C D + CCP C + C ++ + + + + DF C + +G G C +CC D CCP CD + +C + K + TNR S+V+CPD + C TCC + YGCC + NA CC+D + CCP + CD+ + C+
Sbjct: 83 CCPFPEAVACGDGHHCCPRGFHCSADGRSC--------------------FQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCL 261 HSP 5 Score: 72.0182 bits (175), Expect = 7.813e-13 Identity = 39/115 (33.91%), Postives = 52/115 (45.22%), Query Frame = 3
Query: 807 TNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
+G S C F C D CCP+ C C S + N + + CPD++ EC + TCC + D S+GCC + A CC DRV CCPH CD+ C+
Sbjct: 77 VSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGN------------NSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCI 179
BLAST of Granulin b vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 130.954 bits (328), Expect = 6.002e-33 Identity = 103/345 (29.86%), Postives = 146/345 (42.32%), Query Frame = 3
Query: 201 SNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL-IHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHS----PTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
S +VG I CP+ + +C + TCC S+ CC A CC D+ HCCP G+ CD +C+ + K +PA + V VMCP+ +++C D TCC Y CCP NA CCSD CCP+ T CD+ KC S ++ ++ +L H + + + S D Y C + +G G C F+ CC D CCP CD + C + + P L V DN + C S +TCC + + + CC VCCADR CCP +C + C+ + R PL P +
Sbjct: 116 SGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT-PTGTHPLAKKLPAQRTNR---------AVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDM------------EVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREA-PRWDAPLRDPALRQL 437 HSP 2 Score: 72.0182 bits (175), Expect = 6.098e-13 Identity = 39/115 (33.91%), Postives = 52/115 (45.22%), Query Frame = 3
Query: 807 TNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
+G S C F C D CCP+ C C S + N + + CPD++ EC + TCC + D S+GCC + A CC DRV CCPH CD+ C+
Sbjct: 77 VSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGN------------NSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCI 179
BLAST of Granulin b vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 112.849 bits (281), Expect = 1.214e-26 Identity = 91/319 (28.53%), Postives = 126/319 (39.50%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRS 1205
C+ +C T + CC + AV C D HCCP G C A C + + N + CP+++ +C D TCC+ ++ CCP AVCC D CCP TCD + C PH + M + H S + + SD C +T+G G C CCSD + CCPQ C + + EK + + D T C +TCC S+ CC+L +AVCC DR CCP C++K SC + + + P TF RS
Sbjct: 67 CSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ--------------------------RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPC--DNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQ---PATFLARS 354 HSP 2 Score: 105.916 bits (263), Expect = 2.930e-24 Identity = 94/341 (27.57%), Postives = 132/341 (38.71%), Query Frame = 3
Query: 201 SNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLL-LGFKQKAS--KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
S +VG I CP+ + +C + TCC S+ CC AVCC D HCCP G TCD ++ C + M K L + P L+ V + CP++ T C+ T + CCP AVCCSD CCP+ TC + +C + E++ K + +G Q S CP G C+ CC D + CCP C+ K C + V C + C +TCC + + + CC VCCADR CCP +C + C+ + R PL P +
Sbjct: 116 SGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQC--------QRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHF-CHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREA-PRWDAPLRDPALRQL 435
BLAST of Granulin b vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 66.2402 bits (160), Expect = 5.110e-12 Identity = 34/94 (36.17%), Postives = 45/94 (47.87%), Query Frame = 3
Query: 852 CSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDI 1133
C D CCP+ C C S + N + + CPD++ EC + TCC + D S+GCC + A CC DRV CCPH CD+
Sbjct: 106 CGDGHHCCPRGFHCSADGRSCFQRSGN------------NSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDL 187 HSP 2 Score: 49.6766 bits (117), Expect = 2.356e-6 Identity = 24/57 (42.11%), Postives = 32/57 (56.14%), Query Frame = 3
Query: 201 SNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCD 368
S +VG I CP+ + +C + TCC S+ CC A CC D+ HCCP G+ CD
Sbjct: 130 SGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCD 186
BLAST of Granulin b vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 92.4337 bits (228), Expect = 2.019e-20 Identity = 55/136 (40.44%), Postives = 80/136 (58.82%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTC 641
+ T+C++ +TCC+ N++ CC NAVCC D+ HCCP G+TCD + +C+ + +V M K PA K + N+ +EV+CP+ +KC D TCC+ + +Y CCP NAVCC+D CCP TC
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVER----------NQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 2 Score: 75.485 bits (184), Expect = 9.699e-15 Identity = 50/161 (31.06%), Postives = 73/161 (45.34%), Query Frame = 3
Query: 705 IHDSFKYETNFFKIISLLLGF---------------KQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTT-----NRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
+H SF + + KI+ L+L F + + S + C + + G C CC D CCP T CD + +C + + + K P N+ + VVCPD ++C TCC + SYGCC + NAVCCAD + CCP+ C
Sbjct: 66 LHSSFSFIFSI-KIMRLILLFLAVFLCRVTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARC-IGADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 3 Score: 59.6918 bits (143), Expect = 1.230e-9 Identity = 34/82 (41.46%), Postives = 46/82 (56.10%), Query Frame = 3
Query: 996 DNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHF 1241
D ETEC ETCC + D ++GCC + NAVCC DR CCP CD + C+ + +H P+ K+ RKT+ + N F
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGAD---EKHVPMK--KKKPARKTV-ERNQF 174 HSP 4 Score: 58.5362 bits (140), Expect = 3.363e-9 Identity = 25/50 (50.00%), Postives = 31/50 (62.00%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAK 374
+CP+K +KC + TCC + SY CC NAVCC D HCCP G TC +
Sbjct: 178 VCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQ 227 HSP 5 Score: 51.9878 bits (123), Expect = 3.940e-7 Identity = 35/114 (30.70%), Postives = 48/114 (42.11%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASK---SDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKC 893
N + CCP NAVCC D+ CCP TTCD + +C + M ++ F +++ KASK C + G G C CC+D+ CCP C
Sbjct: 116 NTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVVC-----PDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224
BLAST of Granulin b vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 91.6633 bits (226), Expect = 4.337e-20 Identity = 55/136 (40.44%), Postives = 80/136 (58.82%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTC 641
+ T+C++ +TCC+ N++ CC NAVCC D+ HCCP G+TCD + +C+ + +V M K PA K + N+ +EV+CP+ +KC D TCC+ + +Y CCP NAVCC+D CCP TC
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVER----------NQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 2 Score: 75.8702 bits (185), Expect = 8.764e-15 Identity = 47/155 (30.32%), Postives = 68/155 (43.87%), Query Frame = 3
Query: 714 SFKYETNFFKIISLLLGF------------KQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTT-----NRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
SF + ++I L L + + S + C + + G C CC D CCP T CD + +C + + + K P N+ + VVCPD ++C TCC + SYGCC + NAVCCAD + CCP+ C
Sbjct: 71 SFIFSIKIMRLILLFLAVFLCRVTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARC-IGADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 3 Score: 59.6918 bits (143), Expect = 1.561e-9 Identity = 32/76 (42.11%), Postives = 43/76 (56.58%), Query Frame = 3
Query: 996 DNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTL 1223
D ETEC ETCC + D ++GCC + NAVCC DR CCP CD + C+ + +H P+ K+ RKT+
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGAD---EKHVPMK--KKKPARKTV 169 HSP 4 Score: 57.7658 bits (138), Expect = 6.247e-9 Identity = 25/50 (50.00%), Postives = 31/50 (62.00%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAK 374
+CP+K +KC + TCC + SY CC NAVCC D HCCP G TC +
Sbjct: 178 VCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQ 227 HSP 5 Score: 56.225 bits (134), Expect = 1.760e-8 Identity = 61/239 (25.52%), Postives = 88/239 (36.82%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASK---SDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCD----EKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVC------------PDNETE---------CFSWETCCAVSDKS----YGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHF 1181
N + CCP NAVCC D+ CCP TTCD + +C + M ++ F +++ KASK C + G G C CC+D+ CCP C +N + F + P LS P+++ + C + TCC V K+ CC L NA+CC + +CCP C + C + R F
Sbjct: 116 NTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVVC-----PDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQFCSQNFAMIPHLRKFASTSIHRKPAVEFLSEEEEDDDDEESTSSEDPESDVDPIACGVGKTCPAKTTCCNVRGKNGEPKTMCCPLSNAICCEN--TCCPAGYHC-VDGGKCEKHAKTMRTRF 346
BLAST of Granulin b vs. Ensembl Celegans
Match: T02B11.8 (pep chromosome:WBcel235:V:877530:878667:-1 gene:WBGene00044776.1 transcript:T02B11.8a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T02B11.8) HSP 1 Score: 44.669 bits (104), Expect = 8.100e-6 Identity = 21/49 (42.86%), Postives = 26/49 (53.06%), Query Frame = 3
Query: 222 NICPNKRT-KCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTC 365
N+C KRT +C E CC + Y CC + CCP HCCP G +C
Sbjct: 24 NLCDEKRTTQCDEYAVCCPIGNEQYGCCPFTGGTCCPGTNHCCPPGFSC 72
BLAST of Granulin b vs. Ensembl Zebrafish
Match: grnb (granulin b [Source:ZFIN;Acc:ZDB-GENE-030131-7393]) HSP 1 Score: 148.673 bits (374), Expect = 2.559e-38 Identity = 113/350 (32.29%), Postives = 162/350 (46.29%), Query Frame = 3
Query: 174 LAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNI-LEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKA--SKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLST-VVCP------DNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC-VETNSNKRRHFPLT 1190
L W E+ A + V CP+ ++C + TCCQ + CC NAVCC D+ HCCP+G+TCD CV + + A + + K + L A+VN EV+CP+ +KC + TCC+ + +Y CCP AVCCSD+ CCPE TTCD+ + C S+ + + EMA ++ K ++ + S C G C CC D CCP+ T C+ + C D S P ++ST + P D + C TCC +S +GCC L AVCC D V CCPH C++ +C ++S R P+
Sbjct: 80 LDWVEKVEAKLQAVV---CPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSA-NGVSEMATKI------PAVTVLKPKEEVVPCNETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSC-----DDPAGLSVSVPWMEKVSTKPIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMV 414 HSP 2 Score: 130.568 bits (327), Expect = 3.757e-32 Identity = 105/323 (32.51%), Postives = 149/323 (46.13%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILE--AKVNEKDE-VMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFK-YETNFFKIISLLLGFKQ--------KASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLST--VVCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
ICP+ C + TCC T Y CC +A CC D HCC +G+ CD + KCV KT++L+ KV K + V+CP+ +++C D TCC + CCP KNAVCC D+ CCP+ TTCD+ + C S+ + ++ K E N + + + ++ K + C + G G C CCSD + CCP+ T CD ++ C S + +E + P L V P NET C S TCC + S+ CC L AVCC D + CCP C++ +SC
Sbjct: 21 ICPDGGM-CEDGNTCCLTPSGGYGCCPLPHAECCSDHLHCCYQGTLCDLEHSKCVN---------------------KTHVLDWVEKVEAKLQAVVCPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCL--SANGVSEMATKIPAVTVLKPKEEVVPCNETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSC 319 HSP 3 Score: 124.405 bits (311), Expect = 4.308e-30 Identity = 96/319 (30.09%), Postives = 134/319 (42.01%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPAL----KVEDEPKTNILEAKVN-EKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
++ + C TCC+ S+ CC AVCC D+ HCCP+G TCD Q CV+ ++ + PAL +VED ++ +A+ + +D+ C N+ + CCP AVCC D CCP TC+ + CT H I K +K LLG + S CP + G+ G C CC D CCPQ C + C S D S T ++ + C D T C ++CC ++D ++ CC AVCC D CCP KCD K C +
Sbjct: 430 DETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGLPSMAWFRKEPALRETQRVEDR---HMCDAQTSCPRDDTCCFINRI-------------GKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGLHQIPWFT--------KKTARVWKSSDELLGHEDVKCDSSTSCPSGSTCCILPTGQWGCCPLVKAVCCEDHEHCCPQGYICKLELGTCEKASADVSV-----SLTAVQMPEIQC-DTFTRCAHTQSCCRLADSTWACCPYTQAVCCKDMKHCCPMGYKCDPKVQGCTK 718 HSP 4 Score: 119.013 bits (297), Expect = 2.579e-28 Identity = 110/362 (30.39%), Postives = 152/362 (41.99%), Query Frame = 3
Query: 129 VIGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKR----TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSN----VEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITN--GRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCT--LNSTDFDTEKS----KHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
V S +S + W E+ S K PNK+ + C TCC+ + CC AVCC D HCCP GS C+ C + S V M K IPA+ V + K N + CP T CK T ++ CCP AVCC+D+ CCP+ TCD+ C S + MA + + ET ++ + Q + D C N G+ G C CC D CCP C+E+ CT L+ + T+K+ K S V C D+ T C S TCC + +GCC L AVCC D CCP C ++ +C + +++
Sbjct: 314 VAASSCDDPAGLSVSVPWMEK--VSTKPIAPP--PNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMVKKIPAVSVPSQ--------KQNCDETSSCPTGTTCCKLTS-------GSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRS--GLPSMAWFRKEPALR-ETQ--RVEDRHMCDAQTSCPRDDTCCFINRIGKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGLHQIPWFTKKTARVWKSSDELLGHEDVKC-DSSTSCPSGSTCCILPTGQWGCCPLVKAVCCEDHEHCCPQGYICKLELGTCEKASAD 650 HSP 5 Score: 95.9005 bits (237), Expect = 1.109e-20 Identity = 63/216 (29.17%), Postives = 94/216 (43.52%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD-------FDTEKSKH--------SPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS 1163
Y CCP +A CCSD CC + T CD+++ KC + H + + K E ++ + + C + +G G C CC D + CCPQ T CD ++ C + FD + K N + V+CPD ++C TCC + SYGCC + AVCC+D+ CCP CD+ ++C+ N
Sbjct: 40 GGYGCCPLPHAECCSDHLHCCYQGTLCDLEHSKCVNKTHVLDWVE--------KVEAKLQAVV--CPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSANG 245 HSP 6 Score: 82.8037 bits (203), Expect = 2.120e-16 Identity = 52/139 (37.41%), Postives = 68/139 (48.92%), Query Frame = 3
Query: 798 CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS---------NKRRHFPL 1187
C +G G C CCSD CC Q T CD +++KC + D + + +L VVCPD E+EC TCC + D +GCC ++NAVCC DR CCP CD+ + CV T + RR PL
Sbjct: 35 CLTPSGGYGCCPLPHAECCSDHLHCCYQGTLCDLEHSKCVNKTHVLDWVEKVEA----KLQAVVCPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPL 169
BLAST of Granulin b vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 129.028 bits (323), Expect = 1.849e-31 Identity = 100/349 (28.65%), Postives = 143/349 (40.97%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEK----DEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD---FDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS-----NKRRHFPLTFPKRSKFRKTLNDGNHFS 1244
N C + TCC+TK + CC AVCC D HCCP G CD C + +V + +P + + N + V+ D V CP+ T CKD ++ CCP AVCC D CCP C++ C S+ + + +H + + G + S C G G C FS CC+D CCP H KC+ + C ++ ++ +P VV D ++ C + TCC +S GCC AVCC D+ CCP +CD++ SCV+T + H P+ S K + G FS
Sbjct: 641 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-SSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDGSTCCKDK-------DGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPE----SSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFS 977 HSP 2 Score: 120.939 bits (302), Expect = 7.749e-29 Identity = 105/355 (29.58%), Postives = 143/355 (40.28%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA-LKVEDE------PKTNILEAKV---------------------NEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKI--ISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+C N ++C TCCQ + CC AVCC DK HCCPE + CD K KCV + M PA L+ E E P+T A+ +V C N+ C D TCC TK + CCP AVCC D CCP CD+ C S+ + + + K + ++ +S + A+ +D C G C CC D CCP+ KC+ KC + T + + + T + S V C D C TCC D + CC L AVCC D + CCPH +KCD+ SC + + +
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVAC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQK-SDVPCNDT-AACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGS 521 HSP 3 Score: 117.087 bits (292), Expect = 1.582e-27 Identity = 95/330 (28.79%), Postives = 128/330 (38.79%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEK----DEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLL--LGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD--------------TEKSKHSPTTNRLS---TVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
N C + TCC+TK + CC AVCC D HCCP G CD C +V + +P + + + V+ D V CP+ T CKD + CCP AVCC D CCP C++ C S+ M + + K + + S + A+ D C +G C CC D CCP KCD C +S +K SP ++ ++ TV CPD TCC D +GCC L AVCC D + CCPH +KC++ SC
Sbjct: 465 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDK-------DGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDG-------STCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSC 779 HSP 4 Score: 114.39 bits (285), Expect = 1.096e-26 Identity = 104/364 (28.57%), Postives = 142/364 (39.01%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSP---------TTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK--RRHFPLTFPKRSKFRKTLNDGNHFSIL*NSTSTKQNHS 1283
N C + TCC+TK + CC AVCC D HCCP G CD C +V + +P + + K + + D V C N+ C D TCC TK ++ CCP AVCC D CCP+ C+I KC P E + N + A+ D C +G C CC D CCP KCD C S EK P T + +S+ + ++ C TCC D +GCC L AVCC D + CCPH +KC++ SC + + + P+ K+ K T + N T+ + S
Sbjct: 292 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVSWVEKVPVRPIRKQ-KVAVTQVSSVSSD-VPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC-EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGS 650 HSP 5 Score: 102.064 bits (253), Expect = 1.396e-22 Identity = 65/228 (28.51%), Postives = 102/228 (44.74%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP------------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+ CCP + CC D CCPE C +K+ CT++ H+ Q L + + + K ++ F AS+SD CP + + G C + G CSD + CCP +C ++ C ++ TV+C + +EC + TCC D +GCC + AVCC D++ CCP + CD+K CV +++N+ FP R +
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHT-----QPLADRTLAKKPDLPKSFRMI--FSMPASESDITCPDGSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKV--------------KVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCV-SSTNQELPMWDKFPARLR 242 HSP 6 Score: 100.908 bits (250), Expect = 3.817e-22 Identity = 91/357 (25.49%), Postives = 134/357 (37.54%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKT--NILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY-CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS---------------TDFDTEKSKHSPT----------------TNRLSTV----------VCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
CPN C Q+CCQ + CC + + CC D HCCPEG C K C + + + K D PK+ I +E D + CP+ + + ++ +Y CCP + CSD CCP C + C K+ ++L G +D C +G+ G C CC D CCP+ T CD K KC ++ +++ K K T N+++T+ V ++ C TCC D + CC L AVCC D + CCPH +KCD+ SC + + +
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTL--AKKPDLPKSFRMIFSMPASESD-ITCPDGSSCPAEFSCLLMS--TSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK---------------------RKVKVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGS 348 HSP 7 Score: 99.3673 bits (246), Expect = 1.071e-21 Identity = 90/327 (27.52%), Postives = 127/327 (38.84%), Query Frame = 3
Query: 183 REQGFASNK--ETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGF-----KQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF-DTEKSKHSPTTNRLSTVVCPDNET----ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
R+Q A+ K ++ N C + TCC+ K + CC AVCC D HCCP G C+ C + S + K+ L+ P KV CP + T CK+ + CCP AVCC+D CCP C++ + C +I K L LG + S C ++ +G C F CC D + CCP+ +CD + C + + + + H + +VV D + C ETCC S ++GCC AVCC D CCP KC
Sbjct: 710 RKQKVANTKVSPVSSDVACNDTVACPDGSTCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRP-----SGKVKCNATHGCPESSTCCKNIA-------GEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIK--------GDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 1016
BLAST of Granulin b vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 125.561 bits (314), Expect = 2.003e-30 Identity = 103/345 (29.86%), Postives = 143/345 (41.45%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD---FDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS-----NKRRHFPLTFPKRSKFRKTLNDGNHFS 1244
N C + TCC+TK + CC AVCC D HCCP G CD C +V + +P + + K + A D V C N+ C D TCC TK + CCP AVCC D CCP C++ C S+ + + +H + + G + S C G G C FS CC+D CCP H KC+ + C ++ ++ +P VV D ++ C + TCC +S GCC AVCC D+ CCP +CD++ SCV+T + H P+ S K + G FS
Sbjct: 465 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVPWVEKVPVRPIRKQ-KVAVTTASSASSD-VPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPE----SSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFS 801 HSP 2 Score: 120.939 bits (302), Expect = 7.669e-29 Identity = 105/355 (29.58%), Postives = 143/355 (40.28%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA-LKVEDE------PKTNILEAKV---------------------NEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKI--ISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+C N ++C TCCQ + CC AVCC DK HCCPE + CD K KCV + M PA L+ E E P+T A+ +V C N+ C D TCC TK + CCP AVCC D CCP CD+ C S+ + + + K + ++ +S + A+ +D C G C CC D CCP+ KC+ KC + T + + + T + S V C D C TCC D + CC L AVCC D + CCPH +KCD+ SC + + +
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVAC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQK-SDVPCNDT-AACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGS 521 HSP 3 Score: 113.235 bits (282), Expect = 2.548e-26 Identity = 99/329 (30.09%), Postives = 131/329 (39.82%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF---------DTEKSKHSPTTNRLSTVVCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
A ++ ++ N C + TCC+TK + CC AVCC D HCCP G CD C +V + +P + + K + + D V C N+ C D TCC TK ++ CCP AVCC D CCP+ C+I KC P E + N + A+ D C +G C CC D CCP KCD C S K K + TT ++ P N+T C TCC D + CC L AVCC D + CCPH +KC++ SC
Sbjct: 280 AEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVSWVEKVPVRPIRKQ-KVAVTQVSSVSSD-VPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC-EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSC 603 HSP 4 Score: 102.064 bits (253), Expect = 1.215e-22 Identity = 65/228 (28.51%), Postives = 102/228 (44.74%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP------------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+ CCP + CC D CCPE C +K+ CT++ H+ Q L + + + K ++ F AS+SD CP + + G C + G CSD + CCP +C ++ C ++ TV+C + +EC + TCC D +GCC + AVCC D++ CCP + CD+K CV +++N+ FP R +
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHT-----QPLADRTLAKKPDLPKSFRMI--FSMPASESDITCPDGSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKV--------------KVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCV-SSTNQELPMWDKFPARLR 242 HSP 5 Score: 101.679 bits (252), Expect = 1.965e-22 Identity = 87/308 (28.25%), Postives = 120/308 (38.96%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGF-----KQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF-DTEKSKHSPTTNRLSTVVCPDNET----ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
N C + TCC+TK + CC AVCC D HCCP G C+ C + S + K+ L+ P KV CP + T CK+ + CCP AVCC+D CCP C++ + C +I K L LG + S C ++ +G C F CC D + CCP+ +CD + C + + + + H + +VV D + C ETCC S ++GCC AVCC D CCP KC
Sbjct: 553 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRP-----SGKVKCNATHGCPESSTCCKNIA-------GEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIK--------GDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 840 HSP 6 Score: 100.908 bits (250), Expect = 2.955e-22 Identity = 91/357 (25.49%), Postives = 134/357 (37.54%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKT--NILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY-CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS---------------TDFDTEKSKHSPT----------------TNRLSTV----------VCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
CPN C Q+CCQ + CC + + CC D HCCPEG C K C + + + K D PK+ I +E D + CP+ + + ++ +Y CCP + CSD CCP C + C K+ ++L G +D C +G+ G C CC D CCP+ T CD K KC ++ +++ K K T N+++T+ V ++ C TCC D + CC L AVCC D + CCPH +KCD+ SC + + +
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTL--AKKPDLPKSFRMIFSMPASESD-ITCPDGSSCPAEFSCLLMS--TSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK---------------------RKVKVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGS 348 HSP 7 Score: 77.0258 bits (188), Expect = 1.532e-14 Identity = 66/261 (25.29%), Postives = 100/261 (38.31%), Query Frame = 3
Query: 165 SFQLAWREQG---FASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD 938
S + W E+ + + G + N C E TCC+ + CC ++ AVCC D HCCP C+ C+K K+ A + PK ++ K +E+ TCC+ + CCP AVCC D+ CCPE CD++ + C + E+ Q L H + + G + C + G C CC D++ CCP KC +++ D D
Sbjct: 607 SGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKI--AAESTPAPKLDLGVVKCDEQSSC---------SADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQ-LTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCGPGGT--CISAGDLD 853 HSP 8 Score: 70.0922 bits (170), Expect = 2.517e-12 Identity = 49/161 (30.43%), Postives = 77/161 (47.83%), Query Frame = 3
Query: 159 VISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTC 641
VI + + + K +G + ++++ C+ TCC + CC + AVCCPD+ HCCPEG CD ++ CVK + VE+ +L ++P+ +++ V C + +TCC+T + CCP AVCC D CCP C
Sbjct: 689 VIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIR--SNKPQPSVVVKDVQCGGGFSCHDG-------ETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 840
BLAST of Granulin b vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 125.561 bits (314), Expect = 2.547e-30 Identity = 99/349 (28.37%), Postives = 140/349 (40.11%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEK----DEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD---FDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS-----NKRRHFPLTFPKRSKFRKTLNDGNHFS 1244
N C + TCC+TK + CC AVCC D HCCP G CD C +V + +P + + + V+ D V CP+ T CKD + CCP AVCC D CCP C++ C S+ + + +H + + G + S C G G C FS CC+D CCP H KC+ + C ++ ++ +P VV D ++ C + TCC +S GCC AVCC D+ CCP +CD++ SCV+T + H P+ S K + G FS
Sbjct: 465 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDK-------DGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPE----SSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFS 801 HSP 2 Score: 120.939 bits (302), Expect = 8.031e-29 Identity = 105/355 (29.58%), Postives = 143/355 (40.28%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA-LKVEDE------PKTNILEAKV---------------------NEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKI--ISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+C N ++C TCCQ + CC AVCC DK HCCPE + CD K KCV + M PA L+ E E P+T A+ +V C N+ C D TCC TK + CCP AVCC D CCP CD+ C S+ + + + K + ++ +S + A+ +D C G C CC D CCP+ KC+ KC + T + + + T + S V C D C TCC D + CC L AVCC D + CCPH +KCD+ SC + + +
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVAC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQK-SDVPCNDT-AACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGS 521 HSP 3 Score: 114.005 bits (284), Expect = 1.444e-26 Identity = 96/329 (29.18%), Postives = 131/329 (39.82%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSP---------TTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
A ++ ++ N C + TCC+TK + CC AVCC D HCCP G CD C +V + +P + K + +V+ + N+ C D TCC TK ++ CCP AVCC D CCP+ C+I KC P E + N + A+ D C +G C CC D CCP KCD C S EK P T + +S+ + ++ C TCC D +GCC L AVCC D + CCPH +KC++ SC
Sbjct: 280 AEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVSWVEKVPVRPIR---KQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC-EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSC 603 HSP 4 Score: 102.064 bits (253), Expect = 1.271e-22 Identity = 65/228 (28.51%), Postives = 102/228 (44.74%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP------------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+ CCP + CC D CCPE C +K+ CT++ H+ Q L + + + K ++ F AS+SD CP + + G C + G CSD + CCP +C ++ C ++ TV+C + +EC + TCC D +GCC + AVCC D++ CCP + CD+K CV +++N+ FP R +
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHT-----QPLADRTLAKKPDLPKSFRMI--FSMPASESDITCPDGSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKV--------------KVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCV-SSTNQELPMWDKFPARLR 242 HSP 5 Score: 100.908 bits (250), Expect = 3.036e-22 Identity = 91/357 (25.49%), Postives = 134/357 (37.54%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKT--NILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY-CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS---------------TDFDTEKSKHSPT----------------TNRLSTV----------VCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
CPN C Q+CCQ + CC + + CC D HCCPEG C K C + + + K D PK+ I +E D + CP+ + + ++ +Y CCP + CSD CCP C + C K+ ++L G +D C +G+ G C CC D CCP+ T CD K KC ++ +++ K K T N+++T+ V ++ C TCC D + CC L AVCC D + CCPH +KCD+ SC + + +
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTL--AKKPDLPKSFRMIFSMPASESD-ITCPDGSSCPAEFSCLLMS--TSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK---------------------RKVKVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGS 348 HSP 6 Score: 99.3673 bits (246), Expect = 1.128e-21 Identity = 86/308 (27.92%), Postives = 119/308 (38.64%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGF-----KQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF-DTEKSKHSPTTNRLSTVVCPDNET----ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
N C + TCC+ K + CC AVCC D HCCP G C+ C + S + K+ L+ P KV CP + T CK+ + CCP AVCC+D CCP C++ + C +I K L LG + S C ++ +G C F CC D + CCP+ +CD + C + + + + H + +VV D + C ETCC S ++GCC AVCC D CCP KC
Sbjct: 553 NDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRP-----SGKVKCNATHGCPESSTCCKNIA-------GEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIK--------GDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 840 HSP 7 Score: 77.0258 bits (188), Expect = 1.518e-14 Identity = 66/261 (25.29%), Postives = 100/261 (38.31%), Query Frame = 3
Query: 165 SFQLAWREQG---FASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD 938
S + W E+ + + G + N C E TCC+ + CC ++ AVCC D HCCP C+ C+K K+ A + PK ++ K +E+ TCC+ + CCP AVCC D+ CCPE CD++ + C + E+ Q L H + + G + C + G C CC D++ CCP KC +++ D D
Sbjct: 607 SGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKI--AAESTPAPKLDLGVVKCDEQSSC---------SADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQ-LTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCGPGGT--CISAGDLD 853 HSP 8 Score: 70.0922 bits (170), Expect = 2.517e-12 Identity = 49/161 (30.43%), Postives = 77/161 (47.83%), Query Frame = 3
Query: 159 VISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTC 641
VI + + + K +G + ++++ C+ TCC + CC + AVCCPD+ HCCPEG CD ++ CVK + VE+ +L ++P+ +++ V C + +TCC+T + CCP AVCC D CCP C
Sbjct: 689 VIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIR--SNKPQPSVVVKDVQCGGGFSCHDG-------ETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 840
BLAST of Granulin b vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 77.0258 bits (188), Expect = 2.809e-15 Identity = 68/231 (29.44%), Postives = 92/231 (39.83%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFK--QKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC 914
N C + TCC+TK + CC AVCC D HCCP+G C+ KC + + K P V+ N++ +K +V C N+ C D TCC TK + CCP AVCC D CCP C++ C S+ M + + K + + S A+ D C +G C CC D CCP KC+ C
Sbjct: 46 NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTP---VQSTTTPNVIG---TQKSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSC 269 HSP 2 Score: 76.2554 bits (186), Expect = 3.867e-15 Identity = 60/209 (28.71%), Postives = 79/209 (37.80%), Query Frame = 3
Query: 558 HNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD---------TEKSKHSPTTNRLSTVVCPDNETE-CFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
++ CCP AVCC D CCP+ C+I KC P E + N + A+ D C +G C CC D CCP KC+ C S +K K + T ++ P N+T C TCC D + CC L AVCC D + CCPH +KC++ SC
Sbjct: 62 EGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC-EDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSC 269 HSP 3 Score: 67.0106 bits (162), Expect = 6.534e-12 Identity = 52/150 (34.67%), Postives = 68/150 (45.33%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSI 683
N C + TCC+TK + CC AVCC D HCCP G C+ C + S M K +P ++ + K + +A D V C N+ C D TCC TK + CCP AVCC D CCP C++ C S+
Sbjct: 131 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDK-VPVRPIKKQ-KVAVTQASSASSD-VPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSV 276 HSP 4 Score: 57.7658 bits (138), Expect = 5.876e-9 Identity = 47/173 (27.17%), Postives = 67/173 (38.73%), Query Frame = 3
Query: 798 CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK--RRHFPLTFPKRSKFRKTLNDGNHFSIL*NSTSTKQNHS 1283
C G C CC D CCP+ KC+ KC + T + + + T + S V C D C TCC D + CC L AVCC D + CCPH +KC++ SC + + + P+ K+ K T + N T+ + S
Sbjct: 58 CKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQK-SDVPCNDTAA-CPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGS 228
BLAST of Granulin b vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 120.168 bits (300), Expect = 1.984e-28 Identity = 102/350 (29.14%), Postives = 148/350 (42.29%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKAS---------KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRK 1217
ASN+ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K E + E +V D CP+ TCC + Y CC +AVCCSD CCP TTCD+ +KKC S +T ++ + +++++ K + C + +G+ G C CC+D CCP+ C + +S + T K T+ V C D+ C +TCC ++ +GCC + AVCC D CCP +C K H F K R+
Sbjct: 113 ASNQSAV--VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDE-----ERRVPCLDGTFCPDG-------STCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVS-------------------QTGEGPLLPQMPAIREESANQVPCDATTKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFT---KTPALTHEARDVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGY-------TCSGGQCQKGEHSIPLFSKTPALRQ 418 HSP 2 Score: 109.768 bits (273), Expect = 4.676e-25 Identity = 85/297 (28.62%), Postives = 116/297 (39.06%), Query Frame = 3
Query: 243 TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQ--ELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
T C + TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N + K E C + CCP AVCC+D CCPE TC +C+ HSI + L H++ E + C + +G G C + CC D CCP C +C + +P + + V D+ C +TCC ++ +GCC + AVCC D CCP C
Sbjct: 207 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESANQVPCDATTKKETCC--------------RLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPALTHEARDVECDDM----------YSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC--QKGEHSIPLFSKTPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC 469 HSP 3 Score: 105.916 bits (263), Expect = 1.023e-23 Identity = 93/329 (28.27%), Postives = 132/329 (40.12%), Query Frame = 3
Query: 252 TERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNH 1238
T+++TCC+ + CC AVCC D HCCPEG TC Q +C K + ++ PAL E V D CP+ +T C+ ++ CCP AVCC D CCP TC +C HSI ++ + + ET + K + C + +G G C + CC D CCP C ++C + SK + V C D+ C +TCC ++ +GCC + AVCC+D CCP CD + +CV + P +K DG H
Sbjct: 284 TKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSK-MEHSIPWFTKTPALTHEAR--------DVECDDMYSCPDGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSIPLFSKT---PALRQETKYVKCDD-----THSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI-PWFSKTPALKQKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDAR-GTCVLGQ--------FSIPWLTKVPALPFDGTH 571 HSP 4 Score: 83.5741 bits (205), Expect = 1.499e-16 Identity = 89/322 (27.64%), Postives = 118/322 (36.65%), Query Frame = 3
Query: 246 KCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRC--------------KFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK 1169
C + QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + +V C ++ C D +TCC ++ CCP AVCCSD + CCP+ TCD + C SI + K F + CP G RC A C L + + C + L +T S S RL V C D++ CF +TCC + CC VCC D V CCP+ C SC + S +
Sbjct: 430 SCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALK--------------QKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARG-TCVLGQFSIPWLT--------KVPALPFDGTHSICDDTHTCPSGTTCCP-GEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVS----FSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWC-DSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPR 718 HSP 5 Score: 81.2629 bits (199), Expect = 8.217e-16 Identity = 86/293 (29.35%), Postives = 110/293 (37.54%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASK---SDFY--------CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENA 1079
+ C + QTCC+ + CC A AVCC D HCCP G TC Q C K + ++ + PAL+ E + V D C + +T C+ ++ CCP AVCC D CCP TC +C HSI F K +L KQKA D Y C + +G G C + CCSD CCPQ CD + C L F P T D+ C S TCC + CC +E
Sbjct: 352 DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQK-GEHSIPLFSKTPALRQETK--------YVKCDDTHSCADGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI---------------PWFSKTPAL----KQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGT-CVLGQ--FSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEEV 602 HSP 6 Score: 72.7886 bits (177), Expect = 4.161e-13 Identity = 46/136 (33.82%), Postives = 65/136 (47.79%), Query Frame = 3
Query: 810 NGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSD-KSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLT--FPKRSK 1208
G + C + G C D CC + C + + C P +N+ S VVCPD +EC + TCC +SD S+GCC + AVCC D + CCPHN +CD++ C+ + + H P P R K
Sbjct: 76 EGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCI--------------PASNQ-SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCI----SNQDHIPWMSKLPARVK 192
BLAST of Granulin b vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 119.783 bits (299), Expect = 2.319e-28 Identity = 102/350 (29.14%), Postives = 148/350 (42.29%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKAS---------KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRK 1217
ASN+ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K E + E +V D CP+ TCC + Y CC +AVCCSD CCP TTCD+ +KKC S +T ++ + +++++ K + C + +G+ G C CC+D CCP+ C + +S + T K T+ V C D+ C +TCC ++ +GCC + AVCC D CCP +C K H F K R+
Sbjct: 133 ASNQSAV--VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDE-----ERRVPCLDGTFCPDG-------STCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVS-------------------QTGEGPLLPQMPAIREESANQVPCDATTKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFT---KTPALTHEARDVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGY-------TCSGGQCQKGEHSIPLFSKTPALRQ 438 HSP 2 Score: 109.768 bits (273), Expect = 4.442e-25 Identity = 85/297 (28.62%), Postives = 118/297 (39.73%), Query Frame = 3
Query: 243 TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQ--ELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
T C + TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N + K E C + CCP AVCC+D CCPE TC +C+ HSI + L H++ E + C + +G G C + CC D CCP C + +S ++ +P + + V D+ C +TCC ++ +GCC + AVCC D CCP C
Sbjct: 227 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESANQVPCDATTKKETCC--------------RLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPALTHEARDVECDDM----------YSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSK----TPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC 489 HSP 3 Score: 105.531 bits (262), Expect = 1.134e-23 Identity = 93/329 (28.27%), Postives = 132/329 (40.12%), Query Frame = 3
Query: 252 TERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNH 1238
T+++TCC+ + CC AVCC D HCCPEG TC Q +C K + ++ PAL E V D CP+ +T C+ ++ CCP AVCC D CCP TC +C HSI ++ + + ET + K + C + +G G C + CC D CCP C ++C + SK + V C D+ C +TCC ++ +GCC + AVCC+D CCP CD + +CV + P +K DG H
Sbjct: 304 TKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSK-MEHSIPWFTKTPALTHEAR--------DVECDDMYSCPDGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSIPLFSKT---PALRQETKYVKCDD-----THSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI-PWFSKTPALKQKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDAR-GTCVLGQ--------FSIPWLTKVPALPFDGTH 591 HSP 4 Score: 83.5741 bits (205), Expect = 1.727e-16 Identity = 89/321 (27.73%), Postives = 118/321 (36.76%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRC--------------KFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK 1169
C + QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + +V C ++ C D +TCC ++ CCP AVCCSD + CCP+ TCD + C SI + K F + CP G RC A C L + + C + L +T S S RL V C D++ CF +TCC + CC VCC D V CCP+ C SC + S +
Sbjct: 451 CADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALK--------------QKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARG-TCVLGQFSIPWLT--------KVPALPFDGTHSICDDTHTCPSGTTCCP-GEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVS----FSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWC-DSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPR 738 HSP 5 Score: 81.2629 bits (199), Expect = 9.375e-16 Identity = 86/288 (29.86%), Postives = 109/288 (37.85%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASK---SDFY--------CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENA 1079
C + QTCC+ + CC A AVCC D HCCP G TC Q C K + ++ + PAL+ E + V D C + +T C+ ++ CCP AVCC D CCP TC +C HSI F K +L KQKA D Y C + +G G C + CCSD CCPQ CD + C L F P T D+ C S TCC + CC +E
Sbjct: 377 CPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQK-GEHSIPLFSKTPALRQETK--------YVKCDDTHSCADGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI---------------PWFSKTPAL----KQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGT-CVLGQ--FSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEEV 622 HSP 6 Score: 72.7886 bits (177), Expect = 4.334e-13 Identity = 46/136 (33.82%), Postives = 65/136 (47.79%), Query Frame = 3
Query: 810 NGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSD-KSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLT--FPKRSK 1208
G + C + G C D CC + C + + C P +N+ S VVCPD +EC + TCC +SD S+GCC + AVCC D + CCPHN +CD++ C+ + + H P P R K
Sbjct: 96 EGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCI--------------PASNQ-SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCI----SNQDHIPWMSKLPARVK 212
BLAST of Granulin b vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 117.087 bits (292), Expect = 2.029e-27 Identity = 98/347 (28.24%), Postives = 146/347 (42.07%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNIL--EAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKAS-----KSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTF 1193
ASN+ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K E + E +V D CP+ TCC + Y CC +AVCCSD CCP TTCD+ +KKC S +T ++ + +++++ + CP +++ + G C ++ CC D CCP C + +S + + T R+ D+ C ETCC + +GCC + AVCC D + CCP C S +E + P+ F
Sbjct: 113 ASNQSAV--VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDG-------STCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVS-------------------QTGEGPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCVLAEHSIPWMRKTLAQGLKTTRVQC----DDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFTKTPMVF 427 HSP 2 Score: 107.842 bits (268), Expect = 2.332e-24 Identity = 92/335 (27.46%), Postives = 128/335 (38.21%), Query Frame = 3
Query: 243 TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSK----------HSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRK 1217
T C + TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N + CP+ T C + + CCP+ AVCC D CCP TC C + HSI M + L K + + + C + +G+ G C CC+D CCP+ C + +S + T+ P + V C D+ C +TCC ++ +GCC + AVCC D CCP +C K H F K R+
Sbjct: 214 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESANQVPCDATTS----CPDKNTCCHLSS-------EKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCVLAEHSIPWMRKTLAQG--------LKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVARDVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGY-------TCSGGQCQKGEHSIPLFSKTPALRQ 515 HSP 3 Score: 93.5893 bits (231), Expect = 9.943e-20 Identity = 89/346 (25.72%), Postives = 126/346 (36.42%), Query Frame = 3
Query: 189 QGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--------CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHS----------------PTTNRLSTVVCP---------DNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
Q A +E+ + + T C ++ TCC + CC YA AVCC D HCCP G TC CV S M K + + +V D CP +T C+ + CCP AVCC+D CCPE TC +C+ HSI + + + + I ++ + D Y C + +G G C + CC D CCP C + +S ++ P S +CP D+ C +TCC ++ +GCC + AVCC D CCP C
Sbjct: 273 QMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGG--SCVLAEHSIPWMRKTL---------AQGLKTTRVQCDDTASCPEKETCCRLVS-------GKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPM---VFFLSLSLLSILPVVSVARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC 595 HSP 4 Score: 92.4337 bits (228), Expect = 2.400e-19 Identity = 83/311 (26.69%), Postives = 125/311 (40.19%), Query Frame = 3
Query: 243 TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXX-XXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
+ C + +C T CC A C D HCC GS C H C+ P +N + V+CP+ +++C TC ++ +++ CCP AVCC D CCP + CD++ +C S+ I M++ + ++ K++ L ++ +CP + G C + CCSD CCP T CD + KC + + + + V C D T C TCC +S + +GCC AVCC D + CCP C SCV
Sbjct: 63 SGCPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCI---------------------PASN--------QSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKL----PARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPC-DATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCV 337 HSP 5 Score: 92.0485 bits (227), Expect = 3.345e-19 Identity = 103/371 (27.76%), Postives = 142/371 (38.27%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVE---------DE---PKTNILEAKV---NEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASK---SDFY--------CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSK----------HSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNH 1238
+ C + QTCC+ + CC A AVCC D HCCP G TC Q C K + ++ + PAL+ E DE P + + + V C ++ C D +TCC ++ CCP AVCC D CCP TC +C HSI F K +L KQKA D Y C + +G G C + CC D CCP C + +S + ++ ++P +V D+ C TCC + +GCC +E AVCC+D CCP CD + +CV + P +K DG H
Sbjct: 449 DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQK-GEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI---------------PWFSKTPAL----KQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPWYSKTPALKQEGKCNLANTPVNVFAGNIVKCDDSFACKDGYTCCRMVSGVWGCCPIEKAVCCSDHWHCCPQGFTCDAR-GTCVLGQ--------FSIPWLTKVPALPFDGTH 785 HSP 6 Score: 90.1225 bits (222), Expect = 1.308e-18 Identity = 103/366 (28.14%), Postives = 131/366 (35.79%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSI------HEMAQELIHDSFKYETNFFK--IISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN-----------STDFD--------------------------------------TEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK 1169
C + QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + +V C ++ C D +TCC ++ CCP AVCC D CCP TC +C HSI + QE + N F I+ F K D Y C + +G G C CCSD CCPQ CD + C L + FD T S S RL V C D++ CF +TCC + CC VCC D V CCP+ C SC + S +
Sbjct: 557 CADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALK--------------QKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSIPWYSKTPALKQEGKCNLANTPVNVFAGNIVKCDDSFACK----DGYTCCRMVSGVWGCCPIEKAVCCSDHWHCCPQGFTCDARGT-CVLGQFSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEEKEAIETSPSDTSVQGLRLDYVWC-DSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPR 896 HSP 7 Score: 72.7886 bits (177), Expect = 5.736e-13 Identity = 45/142 (31.69%), Postives = 67/142 (47.18%), Query Frame = 3
Query: 813 GRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSD-KSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGN 1235
G + C + G C D CC + C + + C P +N+ S VVCPD +EC + TCC +SD S+GCC + AVCC D + CCPHN +CD++ C+ + L +S+ K + G+
Sbjct: 77 GGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCI--------------PASNQ-SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGD 203
BLAST of Granulin b vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 115.546 bits (288), Expect = 6.262e-27 Identity = 94/325 (28.92%), Postives = 139/325 (42.77%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNIL--EAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKAS-----KSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
ASN+ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K E + E +V D CP+ TCC + Y CC +AVCCSD CCP TTCD+ +KKC S +T ++ + +++++ + CP +++ + G C ++ CC D CCP C + +S + + T R+ D+ C ETCC + +GCC + AVCC D + CCP C
Sbjct: 113 ASNQSAV--VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDG-------STCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVS-------------------QTGEGPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGGSCVLAEHSIPWMRKTLAQGLKTTRVQC----DDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC 405 HSP 2 Score: 110.538 bits (275), Expect = 2.984e-25 Identity = 93/325 (28.62%), Postives = 128/325 (39.38%), Query Frame = 3
Query: 243 TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRK 1217
T C + TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N + CP+ T C + + CCP+ AVCC D CCP TC C + HSI M + L K + + + C + +G+ G C CC+D CCP+ C + +S + T K T+ V C D+ C +TCC ++ +GCC + AVCC D CCP +C K H F K R+
Sbjct: 214 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESANQVPCDATTS----CPDKNTCCHLSS-------EKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCVLAEHSIPWMRKTL--------AQGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFT---KTPALTHEARDVEC-DDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGY-------TCSGGQCQKGEHSIPLFSKTPALRQ 502 HSP 3 Score: 98.9821 bits (245), Expect = 2.088e-21 Identity = 87/315 (27.62%), Postives = 123/315 (39.05%), Query Frame = 3
Query: 189 QGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQ--ELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
Q A +E+ + + T C ++ TCC + CC YA AVCC D HCCP G TC CV S M K + + +V D CP +T C+ + CCP AVCC+D CCPE TC +C+ HSI + L H++ E + C + +G G C + CC D CCP C + +S ++ +P + + V D+ C +TCC ++ +GCC + AVCC D CCP C
Sbjct: 273 QMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCSGG--SCVLAEHSIPWMRKTL---------AQGLKTTRVQCDDTASCPEKETCCRLVS-------GKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPALTHEARDVECDDM----------YSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSK----TPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC 553 HSP 4 Score: 92.4337 bits (228), Expect = 2.663e-19 Identity = 89/338 (26.33%), Postives = 131/338 (38.76%), Query Frame = 3
Query: 243 TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXX-XXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDG 1232
+ C + +C T CC A C D HCC GS C H C+ P +N + V+CP+ +++C TC ++ +++ CCP AVCC D CCP + CD++ +C S+ I M++ + ++ K++ L ++ +CP + G C + CCSD CCP T CD + KC + + + + V C D T C TCC +S + +GCC AVCC D + CCP C SCV L RKTL G
Sbjct: 63 SGCPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCI---------------------PASN--------QSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKL----PARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPC-DATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCV-----------LAEHSIPWMRKTLAQG 353 HSP 5 Score: 72.7886 bits (177), Expect = 5.039e-13 Identity = 45/143 (31.47%), Postives = 67/143 (46.85%), Query Frame = 3
Query: 810 NGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSD-KSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGN 1235
G + C + G C D CC + C + + C P +N+ S VVCPD +EC + TCC +SD S+GCC + AVCC D + CCPHN +CD++ C+ + L +S+ K + G+
Sbjct: 76 EGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCI--------------PASNQ-SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGD 203 HSP 6 Score: 65.855 bits (159), Expect = 8.665e-11 Identity = 87/330 (26.36%), Postives = 119/330 (36.06%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNH 1238
C + QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + +V C ++ C D +TCC ++ CCP AVCC D CCP TC +C HSI ++ + K E N K A K + C C+ +G + CCP C L K P+ L+ C + S + C ++ C AVCC+D CCP CD + +CV + P +K DG H
Sbjct: 515 CADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALK--------------QKAKDVQC-DDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSIPWYSKT---PALKQEGNIVKCDDSF------ACKDGYTC---------CRMVSG-----VWGCCPIEKCCQTHTPLLPLQ---------KCCPSLPILALATCHVQD----SVQDSCTRLQSTHWC---RTAVCCSDHWHCCPQGFTCDAR-GTCVLGQ--------FSIPWLTKVPALPFDGTH 776
BLAST of Granulin b vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 122.865 bits (307), Expect = 9.793e-30 Identity = 99/325 (30.46%), Postives = 139/325 (42.77%), Query Frame = 3
Query: 195 FASNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY---------CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPT----TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
F + +G + CP + +C + TCC S+ CC A CC D+ HCCP G++CD +CV ++ + K PA K V+ V+CP+ KT+C D TCC Y CCP NA+CCSD CCP+ T CD+ KC S Y T+ +++ L G+ K K D C + G G C F+ CC D CCP +C + C + K +P L + D+ T C + TCC ++ +GCC + AVCC+D CCP C
Sbjct: 126 FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKF-PAQKT---------NRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSK----------------NYTTD---LLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC 421 HSP 2 Score: 119.398 bits (298), Expect = 1.416e-28 Identity = 91/308 (29.55%), Postives = 131/308 (42.53%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
+CP+ +T+C + TCC+ Y CC NA+CC D HCCP+ + CD Q KC+ + + KL P + E K + EV CP T C+ + CCP AVCC D CCP C + C + M +++I + K + F + ++ C + +G G C CCSD + CCPQ C + ++ EK TT + D T C +TCC S+ CC+L +AVCC DR CCP C++K +C
Sbjct: 219 VCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKL---------PGYPVKEVKCDM--EVSCPEGYTCCRLNT-------GAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWM-KKVIAPLRLPDPQILKSDTPCDDFT-RCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC 506 HSP 3 Score: 107.071 bits (266), Expect = 2.305e-24 Identity = 89/327 (27.22%), Postives = 133/327 (40.67%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNF-FKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC----VETNSNKRRHFPLTFPKRSKFR 1214
C +C T + CC ++ V C D +HCCP+G C A C + N V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP +CD+ + +C SP H + ++ + F ++ K + C + G+ G C CCSD CCPQ T CD +KC S ++ T+ P + V C D E C TCC ++ ++GCC AVCC D + CCP +C + +C ++ K+ PL P +
Sbjct: 80 CPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC---------------------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCV-SPTGTHTLLKKFPAQKTNRAVSLPFSVVC--PDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCL--SKNYTTDLLTKLP-GYPVKEVKC-DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILK 372 HSP 4 Score: 100.138 bits (248), Expect = 3.887e-22 Identity = 85/316 (26.90%), Postives = 119/316 (37.66%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHF 1181
+ C E TCC+ ++ CC +A AVCC D HCCP G C ++ C M K+I L++ D IL++ D CP N T CK ++ CCP AVCCSD CCP+ TC + C + + + +T +I + CP G C+ CC D + CCP C+ K C DF + ++ V C + C +TCC S + CC VCC D CCP C + C+ + F
Sbjct: 296 DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDP---QILKSDTPCDDFTRCPTNNTCCKLNS-------GDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPAR-----QTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCE-KDVDF-IQPPVLLTLGPKVGNVECGEGHF-CHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMF 592 HSP 5 Score: 67.781 bits (164), Expect = 1.268e-11 Identity = 49/149 (32.89%), Postives = 71/149 (47.65%), Query Frame = 3
Query: 216 VGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKC 662
+G+I ++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K ++ + P + + PK +E C +N+T CKD+ + CCP+ VCC D CCP C + KC
Sbjct: 450 IGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DVDFIQPPVLLTLGPKVGNVECGEGH----FCHDNQTCCKDSA-------GVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKC 581
BLAST of Granulin b vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 75.485 bits (184), Expect = 3.345e-15 Identity = 48/136 (35.29%), Postives = 59/136 (43.38%), Query Frame = 3
Query: 786 SDFYCPIT-NGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK--RRHFP 1184
+ + C +T +G S C FS G C D CCPQ C C S N L V CP ++ EC TCC + D S+GCC + A CC DRV CCPH CD+ CV + FP
Sbjct: 69 AGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSD-------------NPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFP 191 HSP 2 Score: 57.3806 bits (137), Expect = 4.955e-9 Identity = 43/152 (28.29%), Postives = 65/152 (42.76%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL 704
C +C T + CC ++ V C D +HCCP+G C A C ++ D P V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP +CD+ + +C SP H + ++
Sbjct: 67 CPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC----------------FQMSDNPL-----------GAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCV-SPTGTHTLLKKF 190 HSP 3 Score: 53.9138 bits (128), Expect = 7.499e-8 Identity = 41/151 (27.15%), Postives = 56/151 (37.09%), Query Frame = 3
Query: 573 CCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLS----TVVCPDNETEC 1013
CCP V C D Y CCP+ C K C F+ N + G + + S C + +G G C +CC D CCP CD + +C + K + TNR +VVCPD +T+C
Sbjct: 83 CCPFSKGVSCGDGYHCCPQGFHCSADGKSC------------------FQMSDNPLGAVQCP-GSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQC 214 HSP 4 Score: 53.1434 bits (126), Expect = 1.314e-7 Identity = 32/107 (29.91%), Postives = 49/107 (45.79%), Query Frame = 3
Query: 195 FASNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPN 512
F + +G + CP + +C + TCC S+ CC A CC D+ HCCP G++CD +CV ++ + K PA K V+ V+CP+
Sbjct: 113 FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKF-PAQKTNR---------AVSLPFSVVCPD 209
BLAST of Granulin b vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 67.781 bits (164), Expect = 2.544e-12 Identity = 49/149 (32.89%), Postives = 71/149 (47.65%), Query Frame = 3
Query: 216 VGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKC 662
+G+I ++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K ++ + P + + PK +E C +N+T CKD+ + CCP+ VCC D CCP C + KC
Sbjct: 95 IGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DVDFIQPPVLLTLGPKVGNVECGEGH----FCHDNQTCCKDSA-------GVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKC 226 HSP 2 Score: 50.447 bits (119), Expect = 1.525e-6 Identity = 36/112 (32.14%), Postives = 46/112 (41.07%), Query Frame = 3
Query: 867 TCCPQHTKCDEKNNKCTLNSTDFD------------------TEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
T C T+C N C LNS D+ EK TT + D T C +TCC S+ CC+L +AVCC DR CCP C++K +C
Sbjct: 40 TPCDDFTRCPTNNTCCKLNSGDWGCCPIPEGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC 151 HSP 3 Score: 48.1358 bits (113), Expect = 8.290e-6 Identity = 24/59 (40.68%), Postives = 32/59 (54.24%), Query Frame = 3
Query: 216 VGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVK 392
VGN+ + C + QTCC+ + CC Y VCC D HCCP G C A+ KC++
Sbjct: 170 VGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLR 228
BLAST of Granulin b vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 63.5438 bits (153), Expect = 1.404e-10 Identity = 46/142 (32.39%), Postives = 65/142 (45.77%), Query Frame = 3
Query: 243 TKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL--IPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKC 662
T+C TCC+ + CC AVCC D HCCP+G TC A+ + C K + + L IPA + T + + CP +T C K ++ CC +AVCC D+ CCP TC++K + C
Sbjct: 3 TRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGY----CQKGDTMVAGLEKIPARQT-----TPLQIGDIGCDQHTSCPVGQTCCPSLK-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC 128 HSP 2 Score: 62.3882 bits (150), Expect = 3.315e-10 Identity = 40/118 (33.90%), Postives = 56/118 (47.46%), Query Frame = 3
Query: 798 CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTN-RLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
C + +G G C CCSD + CCPQ C + ++ EK TT ++ + C D T C +TCC S+ CC+L +AVCC DR CCP C++K +C
Sbjct: 12 CKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGC-DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC 128 HSP 3 Score: 54.6842 bits (130), Expect = 1.048e-7 Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = 3
Query: 216 VGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILE 473
+G+I ++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K ++ + P + + PK +E
Sbjct: 72 IGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DVDFIQPPVLLTLGPKVGNVE 151
BLAST of Granulin b vs. UniProt/SwissProt
Match: sp|P28799|GRN_HUMAN (Progranulin OS=Homo sapiens OX=9606 GN=GRN PE=1 SV=2) HSP 1 Score: 144.05 bits (362), Expect = 3.504e-36 Identity = 101/327 (30.89%), Postives = 138/327 (42.20%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRS 1205
+CP+ R++C + TCC+ Y CC NA CC D HCCP+ + CD Q KC+ + ++ +PA V D V EV CP+ T C+ + CCP AVCC D CCP TCD + C PH + M + H S + + SD C +T+G G C CCSD + CCPQ C + + EK + + D T C +TCC S+ CC+L +AVCC DR CCP C++K SC + + + P TF RS
Sbjct: 207 MCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD----------VKCDMEVSCPDGYTCCRLQS-------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVP--CDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQ---PATFLARS 511 HSP 2 Score: 128.642 bits (322), Expect = 8.150e-31 Identity = 98/317 (30.91%), Postives = 138/317 (43.53%), Query Frame = 3
Query: 201 SNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL-IHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHS----PTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
S +VG I CP+ + +C + TCC S+ CC A CC D+ HCCP G+ CD +C+ + K +PA + V VMCP+ +++C D TCC Y CCP NA CCSD CCP+ T CD+ KC S ++ ++ +L H + + + S D Y C + +G G C F+ CC D CCP CD + C + + P L V DN + C S +TCC ++ +GCC + AVCC+D CCP C
Sbjct: 116 SGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT-PTGTHPLAKKLPAQRTNR---------AVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDM------------EVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC 410 HSP 3 Score: 103.99 bits (258), Expect = 1.506e-22 Identity = 90/336 (26.79%), Postives = 131/336 (38.99%), Query Frame = 3
Query: 213 TVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLL-LGFKQKAS--KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
TVG++ + C + TCC+ + ++ CC + AVCC D HCCP G TCD ++ C + M K L + P L+ V + CP++ T C+ T + CCP AVCCSD CCP+ TC + +C + E++ K + +G Q S CP G C+ CC D + CCP C+ K C + V C + C +TCC + + + CC VCCADR CCP +C + C+ + R PL P +
Sbjct: 278 TVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQC--------QRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHF-CHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREA-PRWDAPLRDPALRQL 592 HSP 4 Score: 84.7297 bits (208), Expect = 2.714e-16 Identity = 55/199 (27.64%), Postives = 81/199 (40.70%), Query Frame = 3
Query: 573 CCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRL----STVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
CCP AV C D + CCP C + C ++ + + + + DF C + +G G C +CC D CCP CD + +C + K + TNR S+V+CPD + C TCC + YGCC + NA CC+D + CCP + CD+ + C+
Sbjct: 83 CCPFPEAVACGDGHHCCPRGFHCSADGRSC--------------------FQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCL 261 HSP 5 Score: 72.0182 bits (175), Expect = 3.752e-12 Identity = 39/115 (33.91%), Postives = 52/115 (45.22%), Query Frame = 3
Query: 807 TNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
+G S C F C D CCP+ C C S + N + + CPD++ EC + TCC + D S+GCC + A CC DRV CCPH CD+ C+
Sbjct: 77 VSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGN------------NSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCI 179
BLAST of Granulin b vs. UniProt/SwissProt
Match: sp|P23785|GRN_RAT (Progranulin OS=Rattus norvegicus OX=10116 GN=Grn PE=1 SV=3) HSP 1 Score: 128.257 bits (321), Expect = 8.860e-31 Identity = 102/306 (33.33%), Postives = 134/306 (43.79%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEK----SKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
CP + +C + TCC S+ CC A CC D+ HCCP G++CD +C+ ++ P LK +TN A + V+CP+ KT+C D TCC Y CCP NA+CCSD CCP+ T CD+ KC S + +L+ Y N K + S D Y C + G G C F+ CC D CCP +C + C L K S P L V D+ + C S TCC +S +GCC + AVCC D CCP KC
Sbjct: 125 CPGSQFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTH-------PLLKKFPAQRTNRAVASFS----VVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISK-----DYTTDLMTKLPGYPVNEVKC-------DLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLPDPQILKNDVPCDDFSSCPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKC 407 HSP 2 Score: 124.79 bits (312), Expect = 1.693e-29 Identity = 100/339 (29.50%), Postives = 140/339 (41.30%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHF 1241
+CP+ +T+C + TCC+ Y CC NA+CC D HCCP+ + CD Q KC+ + M KL P + E K + EV CP+ T C+ + CCP AVCC D CCP C + C + M + S + K F S ++ C +++G G C CC D + CCPQ KC ++ + EK TT + D T C +TCC S+ CC+L +AVCC DR CCP C++K +C + + + LTF SK HF
Sbjct: 205 VCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKDYTTDLMTKL---------PGYPVNEVKCDL--EVSCPDGYTCCRLNT-------GAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLP-DPQILKNDVPCDDFSSCPS-NNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKCMDEGYCQKGDRMVAGLEKMPVRQTTLLQHGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGSVQPSMDLTF--GSKVGNVECGAGHF 521 HSP 3 Score: 108.997 bits (271), Expect = 3.132e-24 Identity = 93/332 (28.01%), Postives = 139/332 (41.87%), Query Frame = 3
Query: 240 RTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC----VETNSNKRRHFPLTFPKRSKFRKTL 1223
R C + +C T + CC ++ V C D HCCP G C A C + + S +L A V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP +CD+ + +C S P H + ++ F ++ K + C + G+ G C CCSD CCPQ T CD +KC S D+ T+ P ++ V C D E C TCC ++ ++GCC AVCC D + CCP +C +T +C ++ K+ L+ P + +
Sbjct: 64 RDHCPDGYSCLLTVSGTSSCCPFSEGVSCDDGQHCCPRGFHCSADGKSCSQISDS--------------------LLGA-------VQCPGSQFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCIS-PTGTHPLLKKFPAQRTNRAVASFSVVCP--DAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCI--SKDYTTDLMTKLPGYP-VNEVKC-DLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLPDPQILKNDV 361 HSP 4 Score: 90.8929 bits (224), Expect = 3.500e-18 Identity = 77/312 (24.68%), Postives = 117/312 (37.50%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK 1169
+ C + TCC+ ++ CC + AVCC D HCCP G C + C M K+ +L + P IL+ V D CP+N T C+ + ++ CCP AVCC D CCP+ C + C + + + + +T + + CP G C+ CC D + CCP C+ K C ++ + S +++ V C C ++CC S + CC VCC D CCP C K C+ + +
Sbjct: 282 DLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSL---PDPQILKNDVPCDDFSSCPSNNTCCRLSS-------GDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLEKMPVR-----QTTLLQHGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGS--VQPSMDLTFGSKVGNVECGAGHF-CHDNQSCCKDSQGGWACCPYVKGVCCRDGRHCCPIGFHCSAKGTKCLRKKTPR 574 HSP 5 Score: 75.0998 bits (183), Expect = 4.444e-13 Identity = 68/260 (26.15%), Postives = 108/260 (41.54%), Query Frame = 3
Query: 168 FQLAWREQGFAS----NKETVGNICP-NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILE-AKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNST 929
Q+ W ++ AS + + + N P + + C TCC+ + CC AVCC D HCCP+G C + + C K + +++ L+ +T +L+ + CP +T C K ++ CC +AVCC D+ CCP TC++K + C S+ + + +L S K+ ++ G + C + G C + G CC D R CCP C K KC T
Sbjct: 337 LQVPWMKKVTASLSLPDPQILKNDVPCDDFSSCPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKCMDEGY----CQKGD----RMVAGLEKMPVRQTTLLQHGDIGCDQHTSCPVGQTCCPSLK-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGSV-QPSMDLTFGS--------KVGNVECGAGHFCHDNQSCCKDSQGGWACCPYVKGVCCRDGRHCCPIGFHCSAKGTKCLRKKT 572
BLAST of Granulin b vs. UniProt/SwissProt
Match: sp|P28798|GRN_MOUSE (Progranulin OS=Mus musculus OX=10090 GN=Grn PE=1 SV=2) HSP 1 Score: 122.479 bits (306), Expect = 8.678e-29 Identity = 99/325 (30.46%), Postives = 139/325 (42.77%), Query Frame = 3
Query: 195 FASNKETVGNI-CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY---------CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPT----TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKC 1127
F + +G + CP + +C + TCC S+ CC A CC D+ HCCP G++CD +CV ++ + K PA K V+ V+CP+ KT+C D TCC Y CCP NA+CCSD CCP+ T CD+ KC S Y T+ +++ L G+ K K D C + G G C F+ CC D CCP +C + C + K +P L + D+ T C + TCC ++ +GCC + AVCC+D CCP C
Sbjct: 113 FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKF-PAQKT---------NRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSK----------------NYTTD---LLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC 408 HSP 2 Score: 119.398 bits (298), Expect = 1.138e-27 Identity = 91/308 (29.55%), Postives = 131/308 (42.53%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
+CP+ +T+C + TCC+ Y CC NA+CC D HCCP+ + CD Q KC+ + + KL P + E K + EV CP T C+ + CCP AVCC D CCP C + C + M +++I + K + F + ++ C + +G G C CCSD + CCPQ C + ++ EK TT + D T C +TCC S+ CC+L +AVCC DR CCP C++K +C
Sbjct: 206 VCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKL---------PGYPVKEVKCDM--EVSCPEGYTCCRLNT-------GAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWM-KKVIAPLRLPDPQILKSDTPCDDFT-RCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTC 493 HSP 3 Score: 106.686 bits (265), Expect = 1.815e-23 Identity = 89/327 (27.22%), Postives = 133/327 (40.67%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNF-FKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC----VETNSNKRRHFPLTFPKRSKFR 1214
C +C T + CC ++ V C D +HCCP+G C A C + N V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP +CD+ + +C SP H + ++ + F ++ K + C + G+ G C CCSD CCPQ T CD +KC S ++ T+ P + V C D E C TCC ++ ++GCC AVCC D + CCP +C + +C ++ K+ PL P +
Sbjct: 67 CPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC---------------------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCV-SPTGTHTLLKKFPAQKTNRAVSLPFSVVC--PDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCL--SKNYTTDLLTKLP-GYPVKEVKC-DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILK 359 HSP 4 Score: 100.138 bits (248), Expect = 3.282e-21 Identity = 85/316 (26.90%), Postives = 119/316 (37.66%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHF 1181
+ C E TCC+ ++ CC +A AVCC D HCCP G C ++ C M K+I L++ D IL++ D CP N T CK ++ CCP AVCCSD CCP+ TC + C + + + +T +I + CP G C+ CC D + CCP C+ K C DF + ++ V C + C +TCC S + CC VCC D CCP C + C+ + F
Sbjct: 283 DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDP---QILKSDTPCDDFTRCPTNNTCCKLNS-------GDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPAR-----QTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCE-KDVDF-IQPPVLLTLGPKVGNVECGEGHF-CHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMF 579 HSP 5 Score: 67.781 bits (164), Expect = 9.291e-11 Identity = 49/149 (32.89%), Postives = 71/149 (47.65%), Query Frame = 3
Query: 216 VGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKC 662
+G+I ++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K ++ + P + + PK +E C +N+T CKD+ + CCP+ VCC D CCP C + KC
Sbjct: 437 IGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DVDFIQPPVLLTLGPKVGNVECGEGH----FCHDNQTCCKDSA-------GVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKC 568
BLAST of Granulin b vs. UniProt/SwissProt
Match: sp|P28797|GRN_CAVPO (Progranulin OS=Cavia porcellus OX=10141 GN=GRN PE=1 SV=2) HSP 1 Score: 110.538 bits (275), Expect = 1.051e-24 Identity = 93/369 (25.20%), Postives = 134/369 (36.31%), Query Frame = 3
Query: 192 GFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS----PHSIHEMAQ---------------ELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHF 1241
G ICP+ R++C + TCC Y CC NA+CC D HCCP+ + CD +Q +C+ K+ + KL P+ V D V EV CP +T C+ + CCP AVCC D CCPE C + C P + AQ + E + +++S G + C + +G G C S G C +C + + + H + ++ + V C D C +TCC + CC+L +AVCC D CCP C++K SC + PL HF
Sbjct: 210 GLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAKTLLTKL-PSWTVWD----------VECDQEVSCPEGQTCCRLQS-------GKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQGLLQVPWAQKTPAQPSRPSQPSPPGPPGPPSPPGPLRSEISCDEVVSCAPG--------NICCRLASGEWGCCPSSEGYLCM--------------AGERCQVGDRLAPEKMAAHLMSLSQTTDVGC-DQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSCEKAADGAHLAAPLAVGSTGGVMDVACGDRHF 537 HSP 2 Score: 106.301 bits (264), Expect = 3.009e-23 Identity = 86/314 (27.39%), Postives = 125/314 (39.81%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP--------------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
C +C T + CC ++ A+ C D HCCP G C C++ P ++L A E CP++ T C ++ CCP A CC D+ CCP +CD+ + +C ++ S H + +L Y T + G A + CP + +G G C CCSD CCPQ T CD + ++C L+ T +K T + V C D E C +TCC + +GCC AVCC D V CCP +C + ++C
Sbjct: 67 CPIGHSCVLTAAGTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTCIQ-------------------RPDIHLLGAVQCPGGEFECPDSSTCCHMLD-------GSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGS-HPLTTKLPAQRTNY-TGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRC-LSQNKAKTLLTKLPSWT--VWDVEC-DQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTC 348 HSP 3 Score: 99.7525 bits (247), Expect = 3.869e-21 Identity = 70/234 (29.91%), Postives = 107/234 (45.73%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-----VEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC 914
CP +C + TCC S+ CC A CC D+ HCCP G++CD +CV S+ KL PA + E P + V+CP+++++C D TCC+ Y CCP NA+CCSD CCP+ T CD++ +C S A+ L+ + + + + + + C + +G+ G C F CC D CCP+ +C + + C
Sbjct: 126 CPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKL-PAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLS-----QNKAKTLL-----TKLPSWTVWDVECDQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTC 348 HSP 4 Score: 86.6557 bits (213), Expect = 8.326e-17 Identity = 62/228 (27.19%), Postives = 92/228 (40.35%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCT--LNSTDFDTE---------KSKHSPTTN-------RLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK 1169
CCP A+ C D + CCP C C P IH LLG Q +F CP + +G G C +CC D CCP CD + +C L S T+ ++ +P + ++V+CPD+ ++C TCC ++ YGCC + NA+CC+D + CCP + CD++ + C+ N K
Sbjct: 79 GTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTCIQRP-DIH-----------------------LLGAVQ-CPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAK 281 HSP 5 Score: 79.337 bits (194), Expect = 1.907e-14 Identity = 82/347 (23.63%), Postives = 122/347 (35.16%), Query Frame = 3
Query: 213 TVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKK--CTSSPHSIHEMAQEL--IHDSFKYETNFFKIISLL----LGFKQKAS--KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTL 1223
TV ++ ++ C E QTCC+ + + CC + AVCC D HCCPEG C ++ C + V + PA + + ++ + C + C P C + + + C S MA E + D E ++SL +G Q AS CP G C+ CC D + CCP C+ K C + +T + V C D C +TCC S + CC VCC D+ CCP C+ + CV S H+ + P+ + R L
Sbjct: 291 TVWDVECDQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQ-GLLQVPWAQKTPAQPSRPSQPSPPGPPGPPSPPGPL---------------------------RSEISCDEVVSCAPGNICCRLASGEWGCCPSSEGYLCMAGERCQVGDRLAPEKMAAHLMSLSQTTDVGCDQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSCEKAADGAHLAAPLAVGSTGGVMDVACGDRHF-CHDEQTCCRDSRGGWACCPFHQGVCCKDQRHCCPAGFHCESQGTRCVHKKS--LLHWD-SLPRPAAPRPRL 605
BLAST of Granulin b vs. UniProt/SwissProt
Match: sp|Q54QR7|GRN_DICDI (Granulin OS=Dictyostelium discoideum OX=44689 GN=grn PE=3 SV=1) HSP 1 Score: 50.447 bits (119), Expect = 1.989e-6 Identity = 29/70 (41.43%), Postives = 36/70 (51.43%), Query Frame = 3
Query: 939 TEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
T+KS T +V CPD C + TCC+ SD SY CC NA CC+D+ CCP+ C N C
Sbjct: 50 TKKSLELTETQDFGSVKCPDGSL-CPNSNTCCSASDGSYACCPTPNAQCCSDKQHCCPYQFTCGNGGNIC 118
BLAST of Granulin b vs. TrEMBL
Match: A0A2B4SIQ6 (Granulin OS=Stylophora pistillata OX=50429 GN=GRN PE=4 SV=1) HSP 1 Score: 186.808 bits (473), Expect = 1.048e-47 Identity = 122/369 (33.06%), Postives = 173/369 (46.88%), Query Frame = 3
Query: 153 KGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNR---LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL 1250
+G S + + K +CP+ +++C + TCC+ Y CC Y NAVCC D HCCP G TCD C + S++ M + +PAL E V+CP+ +++C D TCC Y CCP+ NAVCCSD CCP TCD+ CT S+ M Q+L + E N ++ G + + + C +++G+ G C + CCSD CCP CD CT S+ E + P R S VVCPD E+EC TCC +S YGCC L NAVCC+D V CCP+ CD+ +C + +S + P K + + N +++
Sbjct: 455 QGSSSIPMVQKLPALMREKSASNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQ-GSSSIPMVQKLPALMRE------------KNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQESSSM-PMMQKL--PALIRERNASNVV--CPGGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDREHCCPSGYTCDVSAGTCTQGSSSI--EMFQKMPAMKRESAASYVVCPDGESECPDGTTCCKLSTGQYGCCPLPNAVCCSDGVHCCPNGYTCDVSAGTCTQGSS--------SIPMVQKLAALMREKNGSNVV 795 HSP 2 Score: 176.407 bits (446), Expect = 4.034e-44 Identity = 114/337 (33.83%), Postives = 161/337 (47.77%), Query Frame = 3
Query: 165 SFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNR---LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
S + + + +CP +++C + TCC+ Y CC Y NAVCC D+ HCCP G TCD C + S++EM + +PA+K E + V+CP+ +++C D TCC Y CCP NAVCCSD CCP TCD+ CT SI M Q+L + E N ++ + + + C +++G+ G C + CCSD CCP CD CT S+ + P R S VVCP ++EC TCC +S YGCC L NAVCC+D CCP+ CD+ +C + +S+
Sbjct: 617 SMPMMQKLPALIRERNASNVVCPGGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDREHCCPSGYTCDVSAGTCTQ-GSSSIEMFQKMPAMKRE------------SAASYVVCPDGESECPDGTTCCKLSTGQYGCCPLPNAVCCSDGVHCCPNGYTCDVSAGTCTQGSSSI-PMVQKLA--ALMREKNGSNVV--CPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSM--PMVQRLPALKREKDASNVVCPGGQSECPDGNTCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSSS 933 HSP 3 Score: 175.637 bits (444), Expect = 7.385e-44 Identity = 115/358 (32.12%), Postives = 163/358 (45.53%), Query Frame = 3
Query: 132 IGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNR-LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKR 1202
+ + +KG S + + +CP+ ++C + TCC+ Y CC NAVCC D HCCP G TCD C + S++ M + +PAL E V+CP+ +++C D TCC Y CCP+ NAVCCSD CCP TCD+ CT SI M Q+L + E N ++ + + + C +++G+ G C + CCSD CCP CD CT S+ + + R S VVCP ++EC TCC +S YGCC NAVCC+DR CCP CD+ +C + +S+ + KR
Sbjct: 369 VSAGTCSKGTTSITFLQKLPAVQRKSDANSVVCPDGESQCPDGTTCCKLSTGQYGCCPLPNAVCCSDGEHCCPNGYTCDVSAGSCTQ-GSSSIPMVQKLPALMRE------------KSASNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSI-PMVQKL--PALMREKNGSNVV--CPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQESSSMPMMQKLPALIRERNASNVVCPGGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDREHCCPSGYTCDVSAGTCTQGSSSIEMFQKMPAMKR 708 HSP 4 Score: 175.252 bits (443), Expect = 9.441e-44 Identity = 118/360 (32.78%), Postives = 165/360 (45.83%), Query Frame = 3
Query: 132 IGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+ + +G S + + K +CP+ +++C + TCC+ Y CC Y NAVCC D HCCP G TCD C + S++ M + +PAL E V+CP +++C D TCC Y CCP+ NAVCCSD+ CCP TCD+ CT SI EM Q++ + K E+ ++ + + C ++ G+ G C CCSD CCP CD CT S+ +K S VVCPD ++EC TCC +S YGCC NAVCC+D CCP+ CD+ +C + +S+ L KR K
Sbjct: 527 VSAGTCTQGSSSIPMVQKLPALMREKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQ-ESSSMPMMQKLPALIRE------------RNASNVVCPGGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDREHCCPSGYTCDVSAGTCTQGSSSI-EMFQKM--PAMKRESAASYVV--CPDGESECPDGTTCCKLSTGQYGCCPLPNAVCCSDGVHCCPNGYTCDVSAGTCTQGSSSIPMVQKLAALMREKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSMPMVQRLPALKREK 868 HSP 5 Score: 170.629 bits (431), Expect = 3.473e-42 Identity = 114/367 (31.06%), Postives = 160/367 (43.60%), Query Frame = 3
Query: 132 IGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQ-KASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDT---EKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKT 1220
+ + +G S + + K +CP+ +++C + TCC+ Y CC Y NAVCC D HCCP G TCD C + S++ M + +PALK E + V+CP +++C D TCC Y CCP NAVCCSD CCP TCD+ C SI + +H + + T + +L K+ + C + +G G C CCSD CCP CD + KC N+ S S + CPD C +ETCC +SD +GCC L +AVCC D CCP +CD+ CV N + P++ K +
Sbjct: 764 VSAGTCTQGSSSIPMVQKLAALMREKNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQ-GSSSMPMVQRLPALKRE------------KDASNVVCPGGQSECPDGNTCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSSSIASLQ---MHAALQAVTYIKAVDGVLCPDKKYQCPNGSTCCRVPSGTYGCCPIKNAVCCSDHIHCCPNGYTCDVEGGKCIKNAEHIKMIANVPSSRSSVDTEQKYIKCPDG-CSCLDYETCCVLSDGRFGCCPLPHAVCCEDHEHCCPSGYRCDLAAEMCVTGNVRLPMLQKIRSPRKQKTPRV 1113 HSP 6 Score: 166.007 bits (419), Expect = 1.319e-40 Identity = 117/393 (29.77%), Postives = 177/393 (45.04%), Query Frame = 3
Query: 69 VIFI*MKYSYSIFAVLVGLAVIGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLS---TVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN--KRRHFPLTFPKRSKFRKTLNDG 1232
+IF+ + + ++V ++ + + A + + ASN +CP+ ++C + TCC+ Y CC AVCC D HCCP G TCD C K + + + KL PA++ + ++ + V+CP+ +++C D TCC Y CCP AVCCSD CCP+ TCD+ CT SI ++ E+N + + + C +++G+ G C CCSD CCP CD C+ +T + P R S +VVCPD E++C TCC +S YGCC L NAVCC+D CCP+ CD+ SC + +S+ + P ++S DG
Sbjct: 116 IIFMILILCLTCIIIVVNAGTCTQGSSSIAMFQKKPAKKRESAASNI-----VCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDISAGTCSKGTTTLILLQKL-PAVQ------------RKSDANSVVCPDGESQCPDGNTCCKLFSGQYGCCPLPKAVCCSDGKHCCPDGYTCDVSAGTCTQGSSSIAMFQKKPAMKRESAESNI-----VCPDGESECPDDTTCCKLSSGQYGCCPLPKAVCCSDGEHCCPNGYTCDVSAGTCSKGTTSITFLQKL--PAVQRKSDANSVVCPDGESQCPDGTTCCKLSTGQYGCCPLPNAVCCSDGEHCCPNGYTCDVSAGSCTQGSSSIPMVQKLPALMREKSASNVVCPDG 483 HSP 7 Score: 164.851 bits (416), Expect = 3.256e-40 Identity = 105/345 (30.43%), Postives = 161/345 (46.67%), Query Frame = 3
Query: 132 IGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTT----NRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
+ + +G S ++ + +CP+ ++C + TCC+ Y CC NAVCC D HCCP G TCD C + S++ M + + AL E V+CP+ +++C D TCC Y CCP+ NAVCCSD CCP TCD+ CT S+ M Q L + K E + ++ G + + + C +++G+ G C CCSD + CCP CD C S+ + + + + V+CPD + +C + TCC V +YGCC ++NAVCC+D + CCP+ CD++ C++
Sbjct: 685 VSAGTCTQGSSSIEMFQKMPAMKRESAASYVVCPDGESECPDGTTCCKLSTGQYGCCPLPNAVCCSDGVHCCPNGYTCDVSAGTCTQ-GSSSIPMVQKLAALMRE------------KNGSNVVCPDGQSECPDGNTCCKLSSGQYGCCPYPNAVCCSDGEHCCPNGYTCDVSAGTCTQGSSSM-PMVQRL--PALKREKDASNVV--CPGGQSECPDGNTCCRLSSGQYGCCPLPNAVCCSDGKHCCPNGYTCDVSAGTCKQGSSSIASLQMHAALQAVTYIKAVDGVLCPDKKYQCPNGSTCCRVPSGTYGCCPIKNAVCCSDHIHCCPNGYTCDVEGGKCIK 1011
BLAST of Granulin b vs. TrEMBL
Match: V4ARK5 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_146967 PE=4 SV=1) HSP 1 Score: 167.933 bits (424), Expect = 7.880e-44 Identity = 109/326 (33.44%), Postives = 158/326 (48.47%), Query Frame = 3
Query: 174 LAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQ-KASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
+ W ++ + + + +CP+++++C + TCC+ Y CC NAVCC D+ HCCP G+TCD KC + ++V K KT+ KV + V+CP+ +++C D TCC Y CCP NAVCCSD+ CCP TTCD KC + + S K + K+ +++ +Q + C ++ G+ G C CCSD CCP T CD KC K S + ++ VVCPD +++C TCC +S YGCC L NAVCC+D++ CCP CD C
Sbjct: 54 VMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSK-----------KTSSQSLKV---ENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKC-------NRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKC 358 HSP 2 Score: 157.918 bits (398), Expect = 3.784e-40 Identity = 108/323 (33.44%), Postives = 152/323 (47.06%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQ-KASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFR 1214
C + TCC+ Y CC NAVCC D+ HCCP G+TCD KC + ++V K KT+ KV + V+CP+ +++C D TCC Y CCP NAVCCSD+ CCP TTCD KC + + S K + K+ +++ +Q + C ++ G+ G C CCSD CCP T CD KC K S + ++ VVCPD +++C TCC +S YGCC L NAVCC+D++ CCP CD C N++ + + K++ +
Sbjct: 1 CPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSK-----------KTSSQSLKV---ENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKC-------NRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKC-----NRQDGTSVMWSKKTSSQ 297 HSP 3 Score: 108.997 bits (271), Expect = 2.498e-22 Identity = 81/276 (29.35%), Postives = 128/276 (46.38%), Query Frame = 3
Query: 174 LAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQ-KASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQH-------TKCDEKNNKCTLNSTDFDTEKSKHSPTTNRL 977
+ W ++ + + + +CP+++++C + TCC+ Y CC NAVCC D+ HCCP G+TCD KC + ++V K KT+ KV + V+CP+ +++C D TCC Y CCP NAVCCSD+ CCP TTCD KC + + S K + K+ +++ +Q + C ++ G+ G C CCSD CCP KCD ++ + S ++ K + + R+
Sbjct: 132 VMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSK-----------KTSSQSLKV---ENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKC-------NRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCDRQDGTSVMWSKKTSSQSLKSADVSYRI 386
BLAST of Granulin b vs. TrEMBL
Match: A0A1A7X3H3 (Granulin b OS=Iconisemion striatum OX=60296 GN=GRNB PE=4 SV=1) HSP 1 Score: 165.622 bits (418), Expect = 1.878e-43 Identity = 109/314 (34.71%), Postives = 160/314 (50.96%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+CP+ C +++TCC+ Y CC +A CC D HCC EG+ CD KCV V + + L + P + L +V V+CP+ +++C D TCC +++ CCP AVCC DK CCPE TTCD+ + KC S + ++L + N + + G K + CP+T+G G C + TCCSD CCP +T CD ++ C + + +T+ VVCPD ++ C TCC +S+ +YGCC L AVCC+D V CCP + +CD+K ++CV N
Sbjct: 20 VCPDGGM-CEDKETCCKNNSGGYGCCPLPHAECCSDHLHCCYEGTLCDLIHQKCVN---KTVSL-PWVRRLPTQQTPPVSQLGGRVKA---VLCPDQESECPDDTTCCQLPDSSWGCCPLPKAVCCEDKRHCCPEGTTCDLVHSKCASLSFQSFPLLEKL---PARRLGNKPAVAVICPGGKSSCPDAFTCCPLTSGEYGCCPYPDATCCSDHSHCCPSNTVCDLEHGVCRSAEILIPLMEKISAVSTD----VVCPDRKSACPDQTTCCLMSNGTYGCCPLPYAVCCSDYVHCCPADTQCDLKHSTCVPAQDN 318 HSP 2 Score: 86.2705 bits (212), Expect = 9.146e-15 Identity = 63/182 (34.62%), Postives = 86/182 (47.25%), Query Frame = 3
Query: 129 VIGSKVAAKGVISFQLAWREQGF-ASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS 671
++ SK A+ SF L + NK V ICP ++ C + TCC Y CC Y +A CC D HCCP + CD + C + E+ LIP ++E +V+CP+ K+ C D TCC+ + Y CCP AVCCSD CCP T CD+K+ C +
Sbjct: 152 LVHSKCASLSFQSFPLLEKLPARRLGNKPAVAVICPGGKSSCPDAFTCCPLTSGEYGCCPYPDATCCSDHSHCCPSNTVCDLEHGVC-----RSAEI--LIP-----------LMEKISAVSTDVVCPDRKSACPDQTTCCLMSNGTYGCCPLPYAVCCSDYVHCCPADTQCDLKHSTCVPA 315
BLAST of Granulin b vs. TrEMBL
Match: A0A3P8VPE0 (Granulin b OS=Cynoglossus semilaevis OX=244447 PE=4 SV=1) HSP 1 Score: 172.555 bits (436), Expect = 4.781e-43 Identity = 113/314 (35.99%), Postives = 152/314 (48.41%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+CP+ C +R TCC+ Y CC +A CC D HCC EG+ CD KCV S + +L + L+ V D+ CP+N TCC N+ CCP AVCC DK+ CCPEATTCDI +KC S+ M ++L K ++ + G K S C +T+G G C ++ CCSD CCP HT+CD K C T K + + V CPD +T C TCC +++ SYGCC + NAVCC+D + CCP CD++ ++CV T N
Sbjct: 49 VCPDGGM-CEDRNTCCKNTVGGYGCCPLPHAECCADHLHCCYEGTLCDLVHKKCVNKTVSLPWLRRLPAKQAFSVSQLSESLKVVVCPGDDATCPDN-------STCCQLHGGNWGCCPLPKAVCCGDKHHCCPEATTCDITKQKCVSASLESFPMLEKLPALQNKDNSDEGVVSVTCPGGKSSCPDSYTCCLLTSGDYGCCPYTEAVCCSDQLHCCPSHTECDVKRGVCRSAQTIVPLLKKVPAVPND----VKCPDKKTACPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTMCDLRHSTCVSTQGN 350 HSP 2 Score: 148.673 bits (374), Expect = 7.263e-35 Identity = 114/362 (31.49%), Postives = 159/362 (43.92%), Query Frame = 3
Query: 165 SFQLAWREQGFASNKETVGN--------ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP--------------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTN-----RLSTVVCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+ L W + A +V +CP C + TCCQ ++ CC AVCC DK HCCPE +TCD + KCV + + M + +PAL+ +D ++ V CP K+ C D+ TCC+ +Y CCP+ AVCCSD+ CCP T CD+K C S+ I+ LL K A +D CP +TNG G C CCSD CCP+ T CD +++ C + T S+ + +LS P NE+ C +TCC +GCC L AVCC D + CCPH C++ ++C + N
Sbjct: 105 TVSLPWLRRLPAKQAFSVSQLSESLKVVVCPGDDATCPDNSTCCQLHGGNWGCCPLPKAVCCGDKHHCCPEATTCDITKQKCVSASLESFPMLEKLPALQNKDNSDEGVV--------SVTCPGGKSSCPDSYTCCLLTSGDYGCCPYTEAVCCSDQLHCCPSHTECDVKRGVCRSA----------------------QTIVPLL--KKVPAVPNDVKCPDKKTACPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTMCDLRHSTCVSTQGNTVTMASEVNAAVTELVLPQLSDASVPCNESVACADGKTCCKKKQGDWGCCPLPEAVCCEDHLHCCPHGTICNLAASTCDDPTYN 434 HSP 3 Score: 135.576 bits (340), Expect = 2.357e-30 Identity = 101/318 (31.76%), Postives = 143/318 (44.97%), Query Frame = 3
Query: 201 SNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNS 1145
S++ V CP ++ C + TCC Y CC Y AVCC D+ HCCP + CD K+ C + A++ V + K +PA+ ++V CP+ KT C D TCC + +Y CCP NAVCCSD CCPE T CD+++ C S+ + MA E+ + S+ + C G G C CC D CCP T C+ + C T N+ T ++ P + S D T C TCC + + CC L AVCC D + CCP++ C++K +
Sbjct: 209 SDEGVVSVTCPGGKSSCPDSYTCCLLTSGDYGCCPYTEAVCCSDQLHCCPSHTECDVKRGVC-RSAQTIVPLLKKVPAV-----------------PNDVKCPDKKTACPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTMCDLRHSTCVSTQGNTVTMASEVNAAVTELVLPQLSDASVPCNESVACADGKTCCKKKQGDWGCCPLPEAVCCEDHLHCCPHGTICNLAASTCDDPTYNTA--VTPWLRNEPAFSLQSDNNKCDETTSCPGNSTCCGAAGGEWACCPLAEAVCCDDHIHCCPYDTTCNLKAQT 506 HSP 4 Score: 120.939 bits (302), Expect = 1.427e-25 Identity = 98/330 (29.70%), Postives = 135/330 (40.91%), Query Frame = 3
Query: 252 TERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC----------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
T TCC+ + CC AVCC D+ HCCP+G C+ Q C K + +PAL+ E + L A++ K MC +N + KDT C + + + CCP AVCCSD CCP TC+ C+ PH I + + + + K + C + G G C CC D + CCPQ C+ K C TL+ +S+ S + + + +C ETCC S + CC AVCC+D CCP CD +T SC NSN+ KR F
Sbjct: 506 TWGTTCCKKTSGLWACCPLPQAVCCSDQEHCCPKGYKCNVAQQTCDKPGGLTLPWLLKVPALQREPT-RAVSLPAQLARK---MCDSNTSCPKDTTCCFMEQTQKWGCCPLPTAVCCSDGVHCCPNGHTCEPHKWSCSRGPHVIPWFTK------LSAYSEPDSVTDVKCDNKSSCAAGTTCCKLPTGEWGCCPLVKAVCCEDHQHCCPQGYTCNMKTGTCDKKDKNVLTHTLHQIKVVQPQSQFS--EDDVGVPCDSTGDLQCSKSETCCKTSPSGWACCPSPQAVCCSDSKYCCPAGFTCDSRTASCSRINSNQLSWDTFFGDKRRDF 823 HSP 5 Score: 71.633 bits (174), Expect = 1.934e-9 Identity = 68/245 (27.76%), Postives = 101/245 (41.22%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNS-YHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS-----PHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTL---NSTDFDT 941
+ T C + TCC + + CC AVCC D HCCP G TC+ + C + + L EP + +V C +NK+ C TCC + CCP AVCC D CCP+ TC++K C H++H++ +++ ++ + + G Q SKS+ C + C CCSD + CCP CD + C+ N +DT
Sbjct: 587 DSNTSCPKDTTCCFMEQTQKWGCCPLPTAVCCSDGVHCCPNGHTCEPHKWSCSR----GPHVIPWFTKLSAYSEP---------DSVTDVKC-DNKSSCAAGTTCCKLPTGEWGCCPLVKAVCCEDHQHCCPQGYTCNMKTGTCDKKDKNVLTHTLHQI--KVVQPQSQFSEDDVGVPCDSTGDLQ-CSKSETCCKTSPSGWACCPSPQAVCCSDSKYCCPAGFTCDSRTASCSRINSNQLSWDT 814 HSP 6 Score: 64.6994 bits (156), Expect = 3.185e-7 Identity = 49/158 (31.01%), Postives = 72/158 (45.57%), Query Frame = 3
Query: 201 SNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXX---XXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCT 665
S ++V ++ + ++ C TCC+ + CC AVCC D HCCP+G TC+ K C K K + + +KV +P++ E D+V P + T T + + CCP AVCCSD CCP TCD + C+
Sbjct: 655 SEPDSVTDVKCDNKSSCAAGTTCCKLPTGEWGCCPLVKAVCCEDHQHCCPQGYTCNMKTGTCDK--KDKNVLTHTLHQIKVV-QPQSQFSE------DDVGVPCDSTGDLQCSKSETCCKTSPSGWACCPSPQAVCCSDSKYCCPAGFTCDSRTASCS 803
BLAST of Granulin b vs. TrEMBL
Match: A0A3P8VL47 (Granulin b OS=Cynoglossus semilaevis OX=244447 PE=4 SV=1) HSP 1 Score: 172.555 bits (436), Expect = 5.489e-43 Identity = 113/314 (35.99%), Postives = 152/314 (48.41%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+CP+ C +R TCC+ Y CC +A CC D HCC EG+ CD KCV S + +L + L+ V D+ CP+N TCC N+ CCP AVCC DK+ CCPEATTCDI +KC S+ M ++L K ++ + G K S C +T+G G C ++ CCSD CCP HT+CD K C T K + + V CPD +T C TCC +++ SYGCC + NAVCC+D + CCP CD++ ++CV T N
Sbjct: 49 VCPDGGM-CEDRNTCCKNTVGGYGCCPLPHAECCADHLHCCYEGTLCDLVHKKCVNKTVSLPWLRRLPAKQAFSVSQLSESLKVVVCPGDDATCPDN-------STCCQLHGGNWGCCPLPKAVCCGDKHHCCPEATTCDITKQKCVSASLESFPMLEKLPALQNKDNSDEGVVSVTCPGGKSSCPDSYTCCLLTSGDYGCCPYTEAVCCSDQLHCCPSHTECDVKRGVCRSAQTIVPLLKKVPAVPND----VKCPDKKTACPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTMCDLRHSTCVSTQGN 350 HSP 2 Score: 148.673 bits (374), Expect = 7.516e-35 Identity = 110/334 (32.93%), Postives = 152/334 (45.51%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP--------------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTN-----RLSTVVCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+CP C + TCCQ ++ CC AVCC DK HCCPE +TCD + KCV + + M + +PAL+ +D ++ V CP K+ C D+ TCC+ +Y CCP+ AVCCSD+ CCP T CD+K C S+ I+ LL K A +D CP +TNG G C CCSD CCP+ T CD +++ C + T S+ + +LS P NE+ C +TCC +GCC L AVCC D + CCPH C++ ++C + N
Sbjct: 133 VCPGDDATCPDNSTCCQLHGGNWGCCPLPKAVCCGDKHHCCPEATTCDITKQKCVSASLESFPMLEKLPALQNKDNSDEGVV--------SVTCPGGKSSCPDSYTCCLLTSGDYGCCPYTEAVCCSDQLHCCPSHTECDVKRGVCRSA----------------------QTIVPLLK--KVPAVPNDVKCPDKKTACPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTMCDLRHSTCVSTQGNTVTMASEVNAAVTELVLPQLSDASVPCNESVACADGKTCCKKKQGDWGCCPLPEAVCCEDHLHCCPHGTICNLAASTCDDPTYN 434 HSP 3 Score: 139.813 bits (351), Expect = 8.311e-32 Identity = 107/340 (31.47%), Postives = 152/340 (44.71%), Query Frame = 3
Query: 201 SNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC------VETNSNKRRHFPLTF 1193
S++ V CP ++ C + TCC Y CC Y AVCC D+ HCCP + CD K+ C + A++ V + K +PA+ ++V CP+ KT C D TCC + +Y CCP NAVCCSD CCPE T CD+++ C S+ + MA E+ + S+ + C G G C CC D CCP T C+ + C T N+ T ++ P + S D T C TCC + + CC L AVCC D + CCP++ C++K +C V + S ++ PLT
Sbjct: 209 SDEGVVSVTCPGGKSSCPDSYTCCLLTSGDYGCCPYTEAVCCSDQLHCCPSHTECDVKRGVC-RSAQTIVPLLKKVPAV-----------------PNDVKCPDKKTACPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTMCDLRHSTCVSTQGNTVTMASEVNAAVTELVLPQLSDASVPCNESVACADGKTCCKKKQGDWGCCPLPEAVCCEDHLHCCPHGTICNLAASTCDDPTYNTA--VTPWLRNEPAFSLQSDNNKCDETTSCPGNSTCCGAAGGEWACCPLAEAVCCDDHIHCCPYDTTCNLKAQTCKDPFGFVPSISWMKKLSPLTI 528 HSP 4 Score: 130.183 bits (326), Expect = 1.319e-28 Identity = 96/309 (31.07%), Postives = 134/309 (43.37%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC--TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
CP+K+T C ++ TCCQ + SY CC NAVCC D HCCPEG+ CD + CV + V M + A V + + +A V + V C + KTCC K ++ CCP AVCC D CCP T C++ C ++ +F +++ K + C G C + CC D CCP T C+ K C + K SP T ++ C D +T C TCC + + CC L AVCC+D+ CCP KC++ +C
Sbjct: 292 CPDKKTACPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTMCDLRHSTCVSTQGNTVTMASEVNA-AVTELVLPQLSDASVPCNESVACADG-------KTCCKKKQGDWGCCPLPEAVCCEDHLHCCPHGTICNLAASTCDDPTYNTAVTPWLRNEPAFSLQSDNNKCDE-----TTSCPGNSTCCGAAGGEWACCPLAEAVCCDDHIHCCPYDTTCNLKAQTCKDPFGFVPSISWMKKLSPLTIKVGNENC-DGQTMCPGGTTCCKKTSGLWACCPLPQAVCCSDQEHCCPKGYKCNVAQQTC 586 HSP 5 Score: 125.176 bits (313), Expect = 5.981e-27 Identity = 103/356 (28.93%), Postives = 147/356 (41.29%), Query Frame = 3
Query: 174 LAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC----------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
++W ++ + VGN + +T C TCC+ + CC AVCC D+ HCCP+G C+ Q C K + +PAL+ E + L A++ K MC +N + KDT C + + + CCP AVCCSD CCP TC+ C+ PH I + + + + K + C + G G C CC D + CCPQ C+ K C TL+ +S+ S + + + +C ETCC S + CC AVCC+D CCP CD +T SC NSN+ KR F
Sbjct: 517 ISWMKK-LSPLTIKVGNENCDGQTMCPGGTTCCKKTSGLWACCPLPQAVCCSDQEHCCPKGYKCNVAQQTCDKPGGLTLPWLLKVPALQREPT-RAVSLPAQLARK---MCDSNTSCPKDTTCCFMEQTQKWGCCPLPTAVCCSDGVHCCPNGHTCEPHKWSCSRGPHVIPWFTK------LSAYSEPDSVTDVKCDNKSSCAAGTTCCKLPTGEWGCCPLVKAVCCEDHQHCCPQGYTCNMKTGTCDKKDKNVLTHTLHQIKVVQPQSQFS--EDDVGVPCDSTGDLQCSKSETCCKTSPSGWACCPSPQAVCCSDSKYCCPAGFTCDSRTASCSRINSNQLSWDTFFGDKRRDF 859 HSP 6 Score: 115.161 bits (287), Expect = 1.499e-23 Identity = 104/347 (29.97%), Postives = 148/347 (42.65%), Query Frame = 3
Query: 180 WREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKC---VKCAKSNVEMGKLIP-ALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPI--------TNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS--TDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRH 1178
W A + ++ N C ++ T C TCC + CC A AVCC D HCCP +TC+ K C S M KL P +KV +E N + MCP T CK T + CCP AVCCSD+ CCP+ C++ + C P + + L + + E + +SL +K S+ CP + G C CCSD CCP C+ C+ + T+ S +S + ++ V C DN++ C + TCC + +GCC L AVCC D CCP C++KT +C + + N H
Sbjct: 440 WLRNEPAFSLQSDNNKC-DETTSCPGNSTCCGAAGGEWACCPLAEAVCCDDHIHCCPYDTTCNLKAQTCKDPFGFVPSISWMKKLSPLTIKVGNE----------NCDGQTMCPGGTTCCKKTS-------GLWACCPLPQAVCCSDQEHCCPKGYKCNVAQQTC-DKPGGL-TLPWLLKVPALQREPT--RAVSLPAQLARKMCDSNTSCPKDTTCCFMEQTQKWGCCPLPTAVCCSDGVHCCPNGHTCEPHKWSCSRGPHVIPWFTKLSAYS-EPDSVTDVKC-DNKSSCAAGTTCCKLPTGEWGCCPLVKAVCCEDHQHCCPQGYTCNMKTGTCDKKDKNVLTH 762
BLAST of Granulin b vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 150.984 bits (380), Expect = 4.279e-39 Identity = 106/335 (31.64%), Postives = 156/335 (46.57%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-VEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDT--EKSKHSPTTNRLSTVVCPDNE-----TECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRS 1205
+CP++ ++C + TCCQ S+ CC NAVCC DK HCCP+G+TCD KCV +V + + PA K E + + + + + ++V CP+ + C D TCC + +Y CCP AVCCSD CCP+ T+CD+ + KC SS ++ E + + + + S+ C +G C CC D CCPQ T C + S D T + PT NR+ P NE T C TCC + ++GCC L AVCC D + CCP N C++ +C S + + +++
Sbjct: 96 VCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSS--NVLEPVTTNLSPVVRVKVT---VESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARST---CVASADLLTLVRWVEKKPTINRVPQ---PANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKT 419 HSP 2 Score: 134.42 bits (337), Expect = 1.784e-33 Identity = 109/316 (34.49%), Postives = 143/316 (45.25%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS-----PHSIHEMAQELIHDSFKYETNFFKII-SLLLGFKQKAS---KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTV-VCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKT 1139
ICP+ C + TCCQT Y CC NA CC D HCC EG+ CD + KCV ++ +P + ++ +A V E CP++ TCC ++ CCP NAVCC DK CCP+ TTCD+ + KC SS P A + + ++N + S + K S CP+ NG G C CCSD CCPQ T CD ++KC ++S + + SP TV P NE+ C S TCC D + CC L AVCC D + CCP C T
Sbjct: 19 ICPDGGM-CDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVN-KTHTLDWVMRVPIR------QPSLPQAVVCPDQESECPDD-------TTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKC-VSSNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARST 318 HSP 3 Score: 107.842 bits (268), Expect = 1.155e-24 Identity = 99/350 (28.29%), Postives = 132/350 (37.71%), Query Frame = 3
Query: 168 FQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPAL-----------KVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS 1163
+ W + A++ + C ++ + C TCC+ K ++ CC AVCC D HCCP+G CD C ++ K PAL K E +E+K + CP + T C K N + CCP AVCC D CCP + TCD C I K E + + G S CP +T G+ G C CC D CCP CD + C ST + T+ V+C D + C +TCC +SD + CC + AVCC D CCP CD K C S
Sbjct: 411 LSVPWVRKTPATSLPPANDQC-DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKK------FNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFK--------KQEAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLVH--THTKEQVMC-DATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARATS 742 HSP 4 Score: 105.531 bits (262), Expect = 7.093e-24 Identity = 89/329 (27.05%), Postives = 132/329 (40.12%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS--PHSIHEMAQELIHDSFKYETNFFKII--SLLLGFKQKAS-KSDFYCPITN--------GRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF------DTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS 1163
+ T C + TCC+ + ++ CC AVCC D HCCP + C+ C A S +P V P T++ A + CP T C+ + CCP AVCC D CCP+ CDI ++ C P + Q + + K I S + + K + CP N + G C CC D CCP CD N C + + + ++ S + ++ V C D+ C + TCC ++ +GCC AVCC D V CCP CD++ +C+ T++
Sbjct: 350 DPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDS-AFSGTAGQLSVPW--VRKTPATSLPPANDQCDETSACPTGTTCCRQKS-------GAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAKVTQGSEVAHGVADVQC-DSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTST 667 HSP 5 Score: 99.3673 bits (246), Expect = 6.600e-22 Identity = 68/217 (31.34%), Postives = 97/217 (44.70%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCT---LNST------------DFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETN 1160
Y CCP NA CCSD CC E T CD+++ KC + H++ + + I + ++ Q++ D C + +G G C CC D CCPQ T CD ++KC L S ++ K + + V CPD + C TCC + + SYGCC + AVCC+D V CCP CD+ + CV +N
Sbjct: 38 GGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQP--------SLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSN 246 HSP 6 Score: 97.4413 bits (241), Expect = 3.245e-21 Identity = 97/359 (27.02%), Postives = 139/359 (38.72%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEK--DEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---------------TLNSTDFDTEKSKHSPTTNRLSTVVCP---DNETECFSWETCCAVSD-KSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKT 1220
K TV ++ N+ C TCC+TK + CC AVCC D HCCP+G+ C + CV ++ ++ L+ VE +P N + NEK CP T CK + CCP AVCC D CCP T C++ C S+ Q + K + C +G C CC D CCPQ KCD + C L S + + + + ++ V D +T C TCC + +GCC L AVCC D CCP++ CD N+C+ + H + + K+ + + T
Sbjct: 262 KVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC--ARSTCV----ASADLLTLV--RWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMES-------GTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGT--AGQLSVPWVRKTPATSLPPANDQCDETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCL------KGHLEIPWFKKQEAKVT 597 HSP 7 Score: 85.8853 bits (211), Expect = 1.898e-17 Identity = 53/146 (36.30%), Postives = 69/146 (47.26%), Query Frame = 3
Query: 798 CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK---RRHFPLTFPKRSKFRKTLN 1226
C +G G C CCSD CC + T CD +++KC + D + VVCPD E+EC TCC + D S+GCC + NAVCC D++ CCP CD+ + CV + RR FP K RK N
Sbjct: 33 CQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSN 178
BLAST of Granulin b vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 150.984 bits (380), Expect = 4.279e-39 Identity = 106/335 (31.64%), Postives = 156/335 (46.57%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-VEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDT--EKSKHSPTTNRLSTVVCPDNE-----TECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRS 1205
+CP++ ++C + TCCQ S+ CC NAVCC DK HCCP+G+TCD KCV +V + + PA K E + + + + + ++V CP+ + C D TCC + +Y CCP AVCCSD CCP+ T+CD+ + KC SS ++ E + + + + S+ C +G C CC D CCPQ T C + S D T + PT NR+ P NE T C TCC + ++GCC L AVCC D + CCP N C++ +C S + + +++
Sbjct: 96 VCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSS--NVLEPVTTNLSPVVRVKVT---VESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARST---CVASADLLTLVRWVEKKPTINRVPQ---PANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKT 419 HSP 2 Score: 134.42 bits (337), Expect = 1.784e-33 Identity = 109/316 (34.49%), Postives = 143/316 (45.25%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS-----PHSIHEMAQELIHDSFKYETNFFKII-SLLLGFKQKAS---KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTV-VCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKT 1139
ICP+ C + TCCQT Y CC NA CC D HCC EG+ CD + KCV ++ +P + ++ +A V E CP++ TCC ++ CCP NAVCC DK CCP+ TTCD+ + KC SS P A + + ++N + S + K S CP+ NG G C CCSD CCPQ T CD ++KC ++S + + SP TV P NE+ C S TCC D + CC L AVCC D + CCP C T
Sbjct: 19 ICPDGGM-CDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVN-KTHTLDWVMRVPIR------QPSLPQAVVCPDQESECPDD-------TTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKC-VSSNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARST 318 HSP 3 Score: 107.842 bits (268), Expect = 1.155e-24 Identity = 99/350 (28.29%), Postives = 132/350 (37.71%), Query Frame = 3
Query: 168 FQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPAL-----------KVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS 1163
+ W + A++ + C ++ + C TCC+ K ++ CC AVCC D HCCP+G CD C ++ K PAL K E +E+K + CP + T C K N + CCP AVCC D CCP + TCD C I K E + + G S CP +T G+ G C CC D CCP CD + C ST + T+ V+C D + C +TCC +SD + CC + AVCC D CCP CD K C S
Sbjct: 411 LSVPWVRKTPATSLPPANDQC-DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKK------FNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFK--------KQEAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLVH--THTKEQVMC-DATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARATS 742 HSP 4 Score: 105.531 bits (262), Expect = 7.093e-24 Identity = 89/329 (27.05%), Postives = 132/329 (40.12%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS--PHSIHEMAQELIHDSFKYETNFFKII--SLLLGFKQKAS-KSDFYCPITN--------GRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF------DTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS 1163
+ T C + TCC+ + ++ CC AVCC D HCCP + C+ C A S +P V P T++ A + CP T C+ + CCP AVCC D CCP+ CDI ++ C P + Q + + K I S + + K + CP N + G C CC D CCP CD N C + + + ++ S + ++ V C D+ C + TCC ++ +GCC AVCC D V CCP CD++ +C+ T++
Sbjct: 350 DPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDS-AFSGTAGQLSVPW--VRKTPATSLPPANDQCDETSACPTGTTCCRQKS-------GAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAKVTQGSEVAHGVADVQC-DSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTST 667 HSP 5 Score: 99.3673 bits (246), Expect = 6.600e-22 Identity = 68/217 (31.34%), Postives = 97/217 (44.70%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCT---LNST------------DFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETN 1160
Y CCP NA CCSD CC E T CD+++ KC + H++ + + I + ++ Q++ D C + +G G C CC D CCPQ T CD ++KC L S ++ K + + V CPD + C TCC + + SYGCC + AVCC+D V CCP CD+ + CV +N
Sbjct: 38 GGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQP--------SLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSN 246 HSP 6 Score: 97.4413 bits (241), Expect = 3.245e-21 Identity = 97/359 (27.02%), Postives = 139/359 (38.72%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEK--DEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC---------------TLNSTDFDTEKSKHSPTTNRLSTVVCP---DNETECFSWETCCAVSD-KSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKT 1220
K TV ++ N+ C TCC+TK + CC AVCC D HCCP+G+ C + CV ++ ++ L+ VE +P N + NEK CP T CK + CCP AVCC D CCP T C++ C S+ Q + K + C +G C CC D CCPQ KCD + C L S + + + + ++ V D +T C TCC + +GCC L AVCC D CCP++ CD N+C+ + H + + K+ + + T
Sbjct: 262 KVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC--ARSTCV----ASADLLTLV--RWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMES-------GTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGT--AGQLSVPWVRKTPATSLPPANDQCDETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCL------KGHLEIPWFKKQEAKVT 597 HSP 7 Score: 85.8853 bits (211), Expect = 1.898e-17 Identity = 53/146 (36.30%), Postives = 69/146 (47.26%), Query Frame = 3
Query: 798 CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNK---RRHFPLTFPKRSKFRKTLN 1226
C +G G C CCSD CC + T CD +++KC + D + VVCPD E+EC TCC + D S+GCC + NAVCC D++ CCP CD+ + CV + RR FP K RK N
Sbjct: 33 CQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSN 178
BLAST of Granulin b vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 111.694 bits (278), Expect = 7.052e-26 Identity = 102/369 (27.64%), Postives = 143/369 (38.75%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVED------------EPKTN--ILEAKVNEKDEVMCP------------NNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKC---TSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFY--CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN-----KRRHFPLTFPKRSKFRKTL 1223
+CP+ ++C + TCC T + CC AVCC DK HCCPE S C KCV + M PA D +PKT+ + N+ + C ++ C D TCC + CCP AVCC D CCP C++ KC + S + ++ I + ET F I + A+ D C + G C CC D CCP KC+ C S K K P L + V ++ C TCC + CC L AVCC D CCPH +KC++ +C + + + K P++ K K + +
Sbjct: 104 MCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRRKDLTNKQPKTSTALKSTTTNKTRNLPCALFSVCTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKGFDIPFYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMK-KTLPLPPALGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKAGV 471 HSP 2 Score: 100.523 bits (249), Expect = 3.199e-22 Identity = 98/385 (25.45%), Postives = 133/385 (34.55%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKAS----KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNST------------------------------------------DFDTEKSKHS--------PTTNRLSTVVCPD-----------NETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTF 1193
N C + TCC+ + CC AVCC D HCCP G C+ C + S M K +P L + V D V CP+ TCC + CCP AVCC D CCP C++ C S+ I + K+ + G A+ C G C G CC D CCP C+ + C N+T + +K++ S P N+L+ PD ++T C + TCC +S +GCC L A CC DR CCP CD+ + SCV+T + PLT+
Sbjct: 314 NDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKTLPLPPA--------LGSDVPCNDTVACPDE-------TTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSV-----PWIKKVPSRPISGKKLPKVKAGVPCNATFACEDESTCCKNDKGGWSCCPLPEGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVPMAGPVKASGPPPPEKKKPKENKANEPAVCCEDGEHCCPAQYKCDKTQTSCIKGDVVIPWYNKLAAESSPDHSVDQSAVKCDSQTSCPAGTTCCEISTGKWGCCPLPEATCCPDREHCCPKGYSCDVNSQSCVKTILLQVETIPLTY 678 HSP 3 Score: 99.7525 bits (247), Expect = 6.143e-22 Identity = 100/399 (25.06%), Postives = 148/399 (37.09%), Query Frame = 3
Query: 159 VISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQT-KHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS-------------------------------PHSIHEMAQELIHDSFKYETNFFKIISLL-LGFKQKASKSDFYCPITNGRSGR--CKFSAGTCCSDLRTCCPQHTKCDEKNNKC--------TLNSTDFDTEKSKHSPTTNRL----------STVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN---KRRHFPL 1187
++ +A G + G CPN++ C ++ TCCQ Y+CC + CC D HCCP G C C + + P D + + V + + VMCP+ ++C TCC T + CCP AVCC+DK CCPE + C + + KC SS P + + + + F + ++ + + +D NG G C CC D CCP KC+ KC L + K P T T CPD TCC S+ + CC L AVCC D + CCPH +KC++ +C + + + ++ PL
Sbjct: 1 MLKLMVALALLGLVT-----GTQCPNQQL-CGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTLICSCF--VNDLLFLNHCCP----NDHECSADSISCVKKAEPVMCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRRKDLTNKQPKTSTALKSTTTNKTRNLPCALFSVCTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKGFDIPFYNVPCNDTAACPDE-------TTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKTLPL 380 HSP 4 Score: 85.5001 bits (210), Expect = 2.169e-17 Identity = 93/408 (22.79%), Postives = 143/408 (35.05%), Query Frame = 3
Query: 165 SFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDE--PKTNI---LEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIK----NKKCTSSPHSIHEMAQELI-----------------------------HDSFKYETNFFKIISLLLGFKQKASKSD----------------------FYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
S + W ++ ++ N C + TCC+ ++CC AVCC D HCCP G C+ C +V K +P+ + + PK A +DE C N + CCP VCC D+ CCP TC+++ +K TS P + A + K +T+ K ++ + + A++S C I+ G+ G C TCC D CCP+ CD + C + + E T R + C C ETCC S ++GCC AVCC+D CCP C+ SC + ++ + F ++ +
Sbjct: 368 SGSVPWMKKTLPLPPALGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCED-PSGSVPWIKKVPSRPISGKKLPKVKAGVPCNATFACEDESTCCKND-------------KGGWSCCPLPEGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVPMAGPVKASGPPPPEKKKPKENKANEPAVCCEDGEHCCPAQYKCDKTQTSCIKGDVVIPWYNKLAAESSPDHSVDQSAVKCDSQTSCPAGTTCCEISTGKWGCCPLPEATCCPDREHCCPKGYSCDVNSQSC-VKTILLQVETIPL--TYIRHLQIPC-GGGISCSDTETCCKTSKTTWGCCPSPKAVCCSDMKHCCPAGYTCE-ANGSCTQATGFNWDNWSVFFSRKKR 756 HSP 5 Score: 79.337 bits (194), Expect = 2.609e-15 Identity = 50/143 (34.97%), Postives = 69/143 (48.25%), Query Frame = 3
Query: 804 ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF-------DTEKSKHSPT-TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+++G C F G CC D CCP C C +N F D E S S + + V+CPD +EC TCC +D +GCC + AVCCAD+V CCP N C + ++ CV +++NK FP R +
Sbjct: 35 LSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTLICSCFVNDLLFLNHCCPNDHECSADSISCVKKAEPVMCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCV-SSTNKELPMWAKFPARLR 176
BLAST of Granulin b vs. Ensembl Cavefish
Match: ENSAMXT00000029960.1 (pep primary_assembly:Astyanax_mexicanus-2.0:23:26786284:26792241:-1 gene:ENSAMXG00000014237.2 transcript:ENSAMXT00000029960.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 106.686 bits (265), Expect = 6.231e-25 Identity = 101/368 (27.45%), Postives = 147/368 (39.95%), Query Frame = 3
Query: 174 LAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-----VEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQE---LIHDSFK-YETNFFKIISLLLGFKQKASK-----SDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDT---------------EKSKHSPTTNRL------STVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
L G S+ T CP+ T C + TCC T Y CC NAVCCP + +CCP+G CD + C K + + L+ E++P T+++E + V C ++ C D TCC T + + CCP+ CC D CCP CD + +C SI + + LI + K E K + ++ +S S + CP GR C ++ G CC D CCP CD + +CTL + + E+++ P + S +V D+ C TCC + CC CC D + CCPH CD + C
Sbjct: 5 LVLLMAGLVSSDIT----CPDGHT-CPDNNTCCLTDQG-YACCPAPNAVCCPGQEYCCPQGYKCDPSSPQ--GCVKQGLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSSPQPAMLIQNKQKAVEETEEKPSATVVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCTLGALSIRSSSQQPAMLIQEQKAVEETEEKPLATLVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCT 363 HSP 2 Score: 97.0561 bits (240), Expect = 1.114e-21 Identity = 74/260 (28.46%), Postives = 112/260 (43.08%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV----------EDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQE---LIHDSFKYETNFFKIISLLLGFKQKASK-----SDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSK 953
C + TCC+T + + CC Y CC D HCCP G CD+ +C++ A S + PA+ + E++P ++E + V C ++ C D TCC T + + CCP+ CC D CCP CD + +CT SI +Q+ LI + E K ++ L+ +S S + CP GR C ++ G CC D CCP CD + +CTL + + +
Sbjct: 118 CPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALS-IRSSSPQPAMLIQNKQKAVEETEEKPSATVVELSDDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCTLGALSIRSSSQQPAMLIQEQKAVEETEEKPLATLVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCTLGALSIRSSSQQ 375 HSP 3 Score: 49.6766 bits (117), Expect = 4.266e-6 Identity = 35/133 (26.32%), Postives = 58/133 (43.61%), Query Frame = 3
Query: 93 SYSIFAVLVGLAVIGSKVAAKGVISFQLAWRE---QGFASNKETVGNICP----NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNIL 470
S S L L++ S +I Q A E + A+ E + P + C + TCC+T + + CC Y CC D HCCP G CD+ +C A S + PA+ ++++ ++++
Sbjct: 258 STSTRCTLGALSIRSSSQQPAMLIQEQKAVEETEEKPLATLVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCTLGALS-IRSSSQQPAMLIQEQVHSSVM 389 HSP 4 Score: 48.9062 bits (115), Expect = 6.070e-6 Identity = 42/175 (24.00%), Postives = 64/175 (36.57%), Query Frame = 3
Query: 744 IISLLLGFKQKASKSDFYCP------------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNK-CTLNSTDF---------DTEKSKHSPTTNRL------STVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFP 1184
+I +L+ SD CP +T+ C CC CCPQ KCD + + C + E+++ P+T+ + S +V D+ C TCC + CC CC D + CCPH CD + C++ + R P
Sbjct: 1 MIVVLVLLMAGLVSSDITCPDGHTCPDNNTCCLTDQGYACCPAPNAVCCPGQEYCCPQGYKCDPSSPQGCVKQGLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSSP 175
BLAST of Granulin b vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 107.071 bits (266), Expect = 2.373e-24 Identity = 96/348 (27.59%), Postives = 133/348 (38.22%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVED----------------EPKTNIL----------EAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCT--------LNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN 1166
+CP+ ++C + TCC T + CC AVCC DK HCCPE S C KCV + M PA D +PKT+ A V+ V C ++ C D TCC + CCP AVCC D CCP C++ KC S+ + + + + K+ ++ C + G C CC D CCP KC+ C + K K P T ++ V C D C TCC S+ + CC L AVCC D CCPH +KC++ +C + + +
Sbjct: 171 MCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPC-DDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPET-KVYNVPCNDTAA-CPDETTCCKDSEGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGS 515 HSP 2 Score: 103.99 bits (258), Expect = 2.075e-23 Identity = 93/360 (25.83%), Postives = 138/360 (38.33%), Query Frame = 3
Query: 213 TVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDE--PKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSPTTN----RLSTVVCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSN-----KRRHFPLTFPKRSKFRKTLN----DGNHF 1241
TV N+ + C + TCC+ + CC AVCC D HCCP G C+ KC +V K +P+ + ++ P+T + N D CP+ TCC + CCP AVCC D CCP C++ C S+ M + + K+ ++ C + G C CC D + CCP KC+ C S +K P + ++ P N T C TCC + CC L AVCC D + CCPH +KC++ +C + + + K+ P+ + K + D +HF
Sbjct: 284 TVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCED-PSGSVPWLKKVPSRPIREKKLPETKVYNVPCN--DTAACPDE-------TTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHF 633 HSP 3 Score: 102.064 bits (253), Expect = 9.466e-23 Identity = 70/255 (27.45%), Postives = 104/255 (40.78%), Query Frame = 3
Query: 456 KTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSI----HEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
K + A + CPN + C + Y CCP CC D CCP C KC +S HS+ + AQ + SF+ ++ ++ + +F C + G C + G C+D CCP +C + C + V+CPD +EC TCC +D +GCC + AVCCAD+V CCP N C + ++ CV +++NK FP R +
Sbjct: 7 KLMVALALLGLVTGTQCPNQQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSLSWTERKPAQAYLAKSFRMISSSTANKDNIICPDNSSCPPEFSCLKLTDKYGCCPVTQGIVCADRNHCCPNDHECSADSISCV-----------------KKAEPVMCPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCV-SSTNKELPMWAKFPARLR 243 HSP 4 Score: 101.293 bits (251), Expect = 1.617e-22 Identity = 97/361 (26.87%), Postives = 136/361 (37.67%), Query Frame = 3
Query: 135 GSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIP-ALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSPTTNR--------LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPL 1187
GS K V S + RE+ K V N+ N C + TCC+ + CC AVCC D HCCP G C+ C + S M KL +K + P+T + N D CP+ T CKD+ + CCP AVCC D CCP C++ C S+ + + + K + C G C CC D CCP KC+ C S EK P + + V C D+ C + TCC + + CC VCC DR+ CCPH+ C+++T +C + ++ P+
Sbjct: 346 GSVPWLKKVPSRPI--REKKLPETK--VYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCN--DTAACPDETTCCKDS-------EGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSC-DSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVPMAGPV 692 HSP 5 Score: 97.0561 bits (240), Expect = 3.747e-21 Identity = 98/409 (23.96%), Postives = 157/409 (38.39%), Query Frame = 3
Query: 159 VISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQT-KHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCT--------------------------------LNSTDFDTEKSKHSPTTNR-----LSTV--VCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL*NSTS 1265
++ +A G + G CPN++ C ++ TCCQ Y+CC + CC D HCCP G C + KC S + + PA + ++ + KD ++CP+N + + +T + Y CCP + C+D+ CCP C + C + M + + + T CP T+G G C CC+D CCP+++ C ++KC L + T + S TTN+ +STV V D+ C TCC + + CC L AVCC D + CCPH +KC++ C + + + + P R K L + +++ N T+
Sbjct: 5 MLKLMVALALLGLVT-----GTQCPNQQL-CGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHS-LSWTERKPAQAYLAK-SFRMISSSTANKDNIICPDNSSCPPEFSCLKLT--DKYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAEPV--MCPDGVSECPVQTT---------------------CCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVP--WLKKVPSRPIREKKLPETKVYNVPCNDTA 378
BLAST of Granulin b vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009302.1 (pep scaffold:Pmarinus_7.0:GL483232:1183:11161:1 gene:ENSPMAG00000008413.1 transcript:ENSPMAT00000009302.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 127.487 bits (319), Expect = 8.679e-32 Identity = 98/371 (26.42%), Postives = 150/371 (40.43%), Query Frame = 3
Query: 132 IGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIIS----LLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLT--FPKRSKFRKTL 1223
+ ++ +G +S + + E IC + ++ C + TCC+ + CC AVCC D HCCPEG+TC+ +C K ++ PAL+V EP I + D+ CP+ TCC ++ CCP AVCC D CCP+ TTC++ + C Q + + +T ++ ++ + D C + +G G C CC D CCPQ T C+ C + + K+ + V C D++ C TCC +GCC L AVCC D CCP C++ SC + P + P R++ L
Sbjct: 338 LPAQTCDQGTLSLPWSTKTPALRVAGEPNVVIC-DDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDK-GTLSLPWSTKTPALRVAGEPNVVICD------DQFSCPDG-------NTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCD----------QGTLSLPWSTKTPALRVAGEPNVVICDDQFSCPDGDTCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSLPWSTKTPALRVAGEPNDVKC-DDQVSCPDQTTCCKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSC----DQGQLSLPWSSKTPARTRLTALL 678 HSP 2 Score: 124.405 bits (311), Expect = 9.077e-31 Identity = 102/361 (28.25%), Postives = 144/361 (39.89%), Query Frame = 3
Query: 153 KGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCP------------DNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPK 1199
KG +S + + E IC + + C + TCC+ + CC AVCC D HCCP+G+TC+ C + ++ PAL+V EP I + D+ CP+ T CK ++ CCP AVCC D CCP+ TTC++ + C S+ + + E N K + Q C G G C CC D CCP+ T C+ C + S +P RL+ ++ P D+ C TCC + +GCC L A CC+D V CCP CD+ SC+ K+ HF PK
Sbjct: 423 KGTLSLPWSTKTPALRVAGEPNVVIC-DDQFSCPDGNTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQ-GTLSLPWSTKTPALRVAGEPNVVICD------DQFSCPDGDTCCKMPS-------GDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSL-PWSTKTPALRVAGEPNDVKCDDQVSCPDQTTC-----CKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSC--DQGQLSLPWSSKTPARTRLTALL-PASAAARGGSVRCDSAYACPDGTTCCQLPQGQWGCCPLPLATCCSDFVHCCPSGYTCDLAQASCI-----KKPHFLTWAPK 754 HSP 3 Score: 119.398 bits (298), Expect = 3.979e-29 Identity = 90/320 (28.12%), Postives = 133/320 (41.56%), Query Frame = 3
Query: 204 NKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIIS----LLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
+K+ ++ + T C+++ TCC + CC AVCC DK HCCPEG+ C+ C A S + PAL++ EP V D V CP+ TCC T ++ CCP AVCC D CCP+ TTC++ + C Q + + +T ++ ++ + + C G C CC D CCPQ T C+ C + + K+ + V+C D+++ C TCC + +GCC L AVCC D CCP C++ C
Sbjct: 127 SKQAASDVQCDAFTSCSDQNTCCILPSGQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTCNHAALS-LPWSTKTPALRMTGEPN------DVQCDDLVGCPDG-------NTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCD----------QGTLSLPWSTKTPALRVAGEPNVVICDDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVIC-DDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERC 421 HSP 4 Score: 111.309 bits (277), Expect = 1.842e-26 Identity = 85/324 (26.23%), Postives = 129/324 (39.81%), Query Frame = 3
Query: 180 WREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFD-TEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
WR + A + CP+ T C + TCC Y CC +AVCC D HCCP+ + CD K KCV K ++ P+ + + + + + ++ + P+ + CCP AVCC DK CCPE T C++ ++ C + S+ + + E N + L+ + C G C CC D CCPQ T C+ C + + K+ + V+C D+++ C TCC + CC L AVCC D CCP C++ +C
Sbjct: 46 WRWR-LARSLAVRPPQCPDG-TSCGDDATCCPLASGQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKCVH-GKLSIPWVSKYPSKQAASDVQCDAFTSCSDQNTCCILPS----------------GQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTCNHAALSL-PWSTKTPALRMTGEPNDVQCDDLV-----GCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVIC-DDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTC 343 HSP 5 Score: 90.1225 bits (222), Expect = 1.435e-19 Identity = 67/213 (31.46%), Postives = 93/213 (43.66%), Query Frame = 3
Query: 588 NAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKII-SLLLGFKQ-----KASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
AVCCSD CCP T CD+ + C S+ + + +F T +++ SL + Q CP+ +G+ G C CCSD CCP++T CD K KC SK+ P+ S V C D T C TCC + +GCC L AVCC D+ CCP C++ + +C H L+ P +K
Sbjct: 1 QAVCCSDGLHCCPSGTVCDLAHGMCQKGKISVPWLEKT---PTFTPRTWRWRLARSLAVRPPQCPDGTSCGDDATCCPLASGQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKCVHGKLSI-PWVSKY-PSKQAASDVQC-DAFTSCSDQNTCCILPSGQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTC--------NHAALSLPWSTK 199 HSP 6 Score: 69.3218 bits (168), Expect = 8.118e-13 Identity = 50/132 (37.88%), Postives = 62/132 (46.97%), Query Frame = 3
Query: 846 TCCSDLRTCCPQHTKCDEKNNKCTLN--STDFDTEKSKHSPTTNRL----STVV----CPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
CCSD CCP T CD + C S + + +P T R S V CPD T C TCC ++ YGCC LE+AVCC+D + CCP N CD+K CV H L+ P SK+
Sbjct: 3 VCCSDGLHCCPSGTVCDLAHGMCQKGKISVPWLEKTPTFTPRTWRWRLARSLAVRPPQCPDG-TSCGDDATCCPLASGQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKCV--------HGKLSIPWVSKY 125
BLAST of Granulin b vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001039.1 (pep scaffold:Pmarinus_7.0:GL484091:11570:24665:-1 gene:ENSPMAG00000000936.1 transcript:ENSPMAT00000001039.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 99.3673 bits (246), Expect = 1.147e-22 Identity = 89/304 (29.28%), Postives = 123/304 (40.46%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETN 1160
C QTCC+++ + CC A CC D HCCP + C Q V LIP LK E L V D V+ C+ +TCC ++ + CC + A CCSD CCP + D+K + C +S I + +E S N G Q + C G G C TCCSD CCP T+C C+ +S E ++ LS + D+ C W+TCC +GCC A CC+D + CCP +C + C ++
Sbjct: 303 CRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVGQ---------CVNSAALIPWLKKEAA-----LSGPVG--DNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLKGQ-CVNSAALIPWLKKEAAL-SGPVGDNLVHC-----GGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSS-----EALSGVAGSSTLSHALPYDSRVYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSS 578 HSP 2 Score: 93.2041 bits (230), Expect = 1.372e-20 Identity = 84/297 (28.28%), Postives = 112/297 (37.71%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLS-TVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIK 1136
C QTCC+++ + CC A CC D HCCP + C Q V LIP LK E + + V+ C +T C+ + CC + A CCSD CCP T C + +C +S I + +E S N G Q + C G G C TCCSD CCP T+C +NS K + + + VV C W+TCC +GCC A CC+D + CCP D+K
Sbjct: 151 CRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ---------CVNSAALIPWLKKEAALSGPVGDNVVHCGGGQYCRRWQTCCRSRC-------GMWGCCNGRRATCCSDGIHCCPSMTRCLV--GQCVNSAALIPWLKKEAAL-SGPVGDNLVHC-----GGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVGQ---CVNSAALIPWLKKEAALSGPVGDNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLK 420 HSP 3 Score: 88.1965 bits (217), Expect = 4.881e-19 Identity = 77/291 (26.46%), Postives = 116/291 (39.86%), Query Frame = 3
Query: 267 CCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAK-QHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLS-TVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDI 1133
CC+++ + CC A CC D HCCP + C + +CV A LIP LK E + + V+ C +T C+ + CC + A CCSD CCP T C + + C+ S ++ ++ + + + + ++ G Q + C G G C TCCSD CCP T+C +NS K + + + VV C W+TCC +GCC A CC+D + CCP +C +
Sbjct: 1 CCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRGQCVNSA-------ALIPWLKKEAALSGPVGDNLVHCGGGQYCRRWQTCCRSRC-------GMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSSEAL-----SVLDYLSREQQDGYNVVH--CGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ---CVNSAALIPWLKKEAALSGPVGDNVVHCGGGQYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLV 267 HSP 4 Score: 85.5001 bits (210), Expect = 3.851e-18 Identity = 79/302 (26.16%), Postives = 114/302 (37.75%), Query Frame = 3
Query: 249 CTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNE---KDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD----FDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDI 1133
C QTCC+++ + CC A CC D HCCP + C C + + E ++ L E + N++ + + + C + + CC + A CCSD CCP T C + +C +S I + +E S N G Q + C G G C TCCSD CCP T+C +NS E + P + L V C + C W+TCC +GCC A CC+D + CCP +C +
Sbjct: 74 CRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSR----SSEALSVLDYLSREQQDGYNVVHCGGGQYCRRWQTCCRSR--------------RGMWGCCNGRRATCCSDGIHCCPSMTRCLV--GQCVNSAALIPWLKKEAAL-SGPVGDNVVHC-----GGGQYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ---CVNSAALIPWLKKEAALSGPVGDNL--VHCGGGQ-YCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLV 343 HSP 5 Score: 50.447 bits (119), Expect = 5.091e-7 Identity = 37/120 (30.83%), Postives = 50/120 (41.67%), Query Frame = 3
Query: 813 GRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDF----DTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETN 1160
G G C TCCSD CCP T+C +NS E + P + L V C + C W+TCC +GCC A CC+D + CCP +C + C ++
Sbjct: 7 GMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRGQCVNSAALIPWLKKEAALSGPVGDNL--VHCGGGQ-YCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSS 123
BLAST of Granulin b vs. Ensembl Nematostella
Match: EDO27824 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T870]) HSP 1 Score: 72.7886 bits (177), Expect = 1.197e-14 Identity = 71/236 (30.08%), Postives = 97/236 (41.10%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFK---IISLLLGFKQKASKS-----DFY---CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNN 908
N + C + +TCC+ SY CC NAVCC D HCCP G CD C AK + M ++ + + E K KD C +T C Y CCP +AVCC+D CCP TC+ K+ C H + M Q+ H S K II + F + + + Y C ++ + C +CCSD CCPQ CD++
Sbjct: 10 NDGSHCYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDM-AKDSTPMVSYHATVQ-----RAGVGEIKC--KDGSHCNTGETCCLVGP-------TKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVL-PMFQK--HPSVHPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICDDQKG 227 HSP 2 Score: 71.633 bits (174), Expect = 2.556e-14 Identity = 58/206 (28.16%), Postives = 81/206 (39.32%), Query Frame = 3
Query: 561 NNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELI-HDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS---TDFDTEKSKHSPTTNRLSTVVCPDNETE------------CFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCD 1130
+Y CCP NAVCCSD CCP CDI + C +MA++ S+ + + + + C + + G C + CC+D++ CCP C++K+ C F S H P T V D+ C + TCC S CC L NA CC+D + CCP CD
Sbjct: 27 GSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTC--------DMAKDSTPMVSYHATVQRAGVGEIKCKDGSHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVLPMFQKHPSVH-PRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICD 223 HSP 3 Score: 66.6254 bits (161), Expect = 1.141e-12 Identity = 48/141 (34.04%), Postives = 64/141 (45.39%), Query Frame = 3
Query: 798 CPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNR--LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFR 1214
C ++ G G C CCSD + CCP CD ++ C + + D S H+ T R + + C D + C + ETCC V YGCC L +AVCCAD CCP C+ K+ C + RH F K
Sbjct: 22 CELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDM-AKDSTPMVSYHA-TVQRAGVGEIKCKDG-SHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHC-----QRGRHVLPMFQKHPSVH 154 HSP 4 Score: 59.3066 bits (142), Expect = 4.487e-10 Identity = 30/82 (36.59%), Postives = 45/82 (54.88%), Query Frame = 3
Query: 996 DNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC-VETNSNKRRHFPLTFPKRSKFRKTLNDGNH 1238
++ + C+ +ETCC +S SYGCC L NAVCC+D CCP CDI +++C + +S + T + DG+H
Sbjct: 10 NDGSHCYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDMAKDSTPMVSYHATVQRAGVGEIKCKDGSH 91 HSP 5 Score: 55.4546 bits (132), Expect = 8.284e-9 Identity = 46/162 (28.40%), Postives = 63/162 (38.89%), Query Frame = 3
Query: 204 NKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEV-MCPNNXXXXXXXXXXX----XXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSP 674
+ VG I + C +TCC Y CC +AVCC D HCCP G TC+ K C + + + M + P++ P+ + + V + P + CCP NA CCSD CCP+ CD K C P
Sbjct: 77 QRAGVGEIKCKDGSHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQR-GRHVLPMFQKHPSV----HPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICD-DQKGCVKQP 232
BLAST of Granulin b vs. Ensembl Medaka
Match: grnb (granulin precursor [Source:NCBI gene;Acc:101166910]) HSP 1 Score: 149.058 bits (375), Expect = 2.034e-38 Identity = 120/387 (31.01%), Postives = 171/387 (44.19%), Query Frame = 3
Query: 99 SIFAVLVGLAVI----GSKVAAKGVISFQLAWREQGFA---SNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD----FDTEKSKHSPTTNRLSTVVCPDNET-ECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLT-----FPKRSK 1208
++ VLVGL+ V A G F + GF + ICP++ ++C + TCCQ S+ CC + AVCC D+ HCCPEG+TCD KC + M + +PA +V P + A + V+CP K+ C ++ TCC+ ++ CCP+ A CCSD CCP TTCD++ C S E E I + L + C + NG G C CCSD CCP+ T CD ++ C + S P T+ + + P N + C TCC S +GCC L AVCC D + CCPH C++ ++C + + + PL FP RS+
Sbjct: 3 ALCVVLVGLSAALLCPDGAVCADGHKCFLNS--HGGFTCPLPHTRVGAVICPDQESECPDETTCCQLPDGSWGCCPFPKAVCCEDQRHCCPEGTTCDLVHSKCASPSLQAFPMLQKVPAQRVGTLP---VFAAAAS----VVCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVCKSG-----EARVEPIRAVSASPEDV-----LCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTHPVRGLAVPCNASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALT--PLLSNTPGFPYRSE 368 HSP 2 Score: 130.954 bits (328), Expect = 2.878e-32 Identity = 100/372 (26.88%), Postives = 152/372 (40.86%), Query Frame = 3
Query: 129 VIGSKVAAKGVISFQL-----AWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLND 1229
++ SK A+ + +F + A R +CP ++ C TCC + CC Y A CC D HCCP +TCD ++ C KS E + + A ++V+CP+ ++ C D TCC + + CCP NAVCCSD CCPE TTCD+ + C S+ E + + +T+ + +++ + C + G+ G C CC D CCP T C+ + C S T ++P S D C TCC + CC L AVCC D + CCP+ C+++ +C + S P KR + + +
Sbjct: 98 LVHSKCASPSLQAFPMLQKVPAQRVGTLPVFAAAASVVCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVC----KSG--------------EARVEPIRAVSASPEDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGT-PPKTHPVRGLAVPCNASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYRSENGRCDESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETC-DDPSGLSPPLPW-LTKRPRLTAGMEE 448 HSP 3 Score: 112.464 bits (280), Expect = 4.071e-26 Identity = 91/328 (27.74%), Postives = 131/328 (39.94%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETE--CFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
K C R TCC+ + CC AVCC D HCCP+G C+A C + ++ + +PAL+ + +T +E K + + CP + T C +T + CCP AVCC D CCP +C C+ H + + T + + K + CP+ G G C CC+D CCPQ C+ + C + S+ P + + V D E C TCC +S ++GCC AVCC D CCP CD+K C T + L +R+ F
Sbjct: 452 GKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLPALQ-QRSGQTGPVENKCDA--QTSCPKDTTCCFMEQTL------KWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSRGSHVVPWFTK------VGALTEPSGVTDVKCDNKSSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCEKKAGQ-GLPLSRVVPAQPQDTQDVPCDGTGEFKCPKQNTCCRISATAWGCCPSPGAVCCMDSKHCCPAGYSCDLKAGGC--TLGPRLSWDSLIGGRRTDF 761 HSP 4 Score: 108.227 bits (269), Expect = 9.378e-25 Identity = 104/361 (28.81%), Postives = 139/361 (38.50%), Query Frame = 3
Query: 153 KGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDS--FKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECF------SWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKF 1211
+GV A E A + +CP++R+ C + TCCQ + ++ CC NAVCC D HCCPEG+TCD CV A E ++P PKT+ + V C N C D TCC + CCP AVCC D CCP T C++ C S L+ ++ F Y + + C G C CC D CCP T C+ + C D + S P + + E +C TCC S + CC L AVCC+D CCP KC+ +C +R P + P R K
Sbjct: 185 RGVCKSGEARVEPIRAVSASPEDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEA-VMVPVSGT--PPKTHPVRGLA-----VPC-NASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGS---ALTPLLSNTPGFPYRSENGRCDE-----SASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETC-----DDPSGLSPPLPWLTKRPRLTAGMEEEKCGKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTC------ERPGGP-SLPWRQKL 516 HSP 5 Score: 73.9442 bits (180), Expect = 1.192e-13 Identity = 48/172 (27.91%), Postives = 75/172 (43.60%), Query Frame = 3
Query: 165 SFQLAW-REQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPH 677
S + W + G + V ++ + ++ C TCC + + CC AVCC D HCCP+ TC+ + C K A + + +++PA + + + + E CP T C+ + + CCP AVCC D CCP +CD+K CT P
Sbjct: 589 SHVVPWFTKVGALTEPSGVTDVKCDNKSSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCEKKAGQGLPLSRVVPAQPQDTQ------DVPCDGTGEFKCPKQNTCCRISA-------TAWGCCPSPGAVCCMDSKHCCPAGYSCDLKAGGCTLGPR 747 HSP 6 Score: 69.707 bits (169), Expect = 2.694e-12 Identity = 70/257 (27.24%), Postives = 104/257 (40.47%), Query Frame = 3
Query: 174 LAWREQGFASNKETVGNICP-----NKRTKCTERQTCCQTKHN-SYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL-IHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN 923
L WR++ + ++ G P + +T C + TCC + + CC AVCC D HCCP G +C + C + ++P K L D V C +NK+ C TCC + + CCP AVCC+D CCP++ TC+++ C + Q L + + + + + K K + C I+ G C CC D + CCP CD K CTL
Sbjct: 510 LPWRQK-LPALQQRSGQTGPVENKCDAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSR-------GSHVVPWFT-----KVGALTEPSGVTD-VKC-DNKSSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCE------KKAGQGLPLSRVVPAQPQDTQDVPCDGTGEFKCPKQNTCCRISATAWGCCPSPGAVCCMDSKHCCPAGYSCDLKAGGCTLG 745
BLAST of Granulin b vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 122.479 bits (306), Expect = 2.015e-29 Identity = 91/339 (26.84%), Postives = 135/339 (39.82%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN--STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNS--NKRRHFPLTFPKRSKFRKTLNDGNH 1238
+ T C ++ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V CP+ T CK + CCP AVCC D CCP+ TC++ + C S+ ++ + K+ + C G G C F CC D CCP+ C+ C + S + S + ++ V C D T C TCC D ++GCC L AVCC D CCP CD+ T SC++ + + P + S + + G
Sbjct: 534 DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQTTCCKTQ-------EGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV-----PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVSVKMEDVPC-DGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQE 853 HSP 2 Score: 118.242 bits (295), Expect = 5.849e-28 Identity = 87/326 (26.69%), Postives = 134/326 (41.10%), Query Frame = 3
Query: 177 AWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN--STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
A+ E + + + +I N+ C ++ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V CP+ TCC T+ + CCP AVCC D CCP+ TC++ + C S+ ++ + K+ + C G G C F CC D CCP+ C+ C + S + S + ++ V C D T C TCC + +GCC AVCC D + CCP + C++ +C
Sbjct: 278 AFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV-----PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPC-DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC 584 HSP 3 Score: 114.775 bits (286), Expect = 7.923e-27 Identity = 95/360 (26.39%), Postives = 145/360 (40.28%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNIL------------------------EAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC-----------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+ T C ++ TCC+TK ++ CC AVCC D HCCP G+TCD C+K A V M K +PA + ++ E K +E+ + C ++ + T C + + + CCP AVCC+D CCP C ++ C I + DS + +++F + ++ + + C + G G C CC D CCP+ CD + C L ++S+H P L+ D+ C ETCC S ++GCC AVCC D CCP +C K SC++ ++ F + K
Sbjct: 771 DGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLK-ASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAV-----HQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRC---DDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCIKNTGLHWLNWHTFFTNKRK 1120 HSP 4 Score: 110.153 bits (274), Expect = 2.657e-25 Identity = 102/376 (27.13%), Postives = 140/376 (37.23%), Query Frame = 3
Query: 162 ISFQLAWREQGFASNKETVG----NICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNE-KDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDS-------------------------FKYETNFFKIISLLLG--FKQKASKSDFYCPIT-------------NGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN--STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
+S L + F + +G NICP+ +++C +C + Y CC A V CPD HCCPEG C CVK ++ KT + + V+E DE C N+ + CCP AVCC DK CCPE TTCD++ KC SS M + + F T + + L G +AS D C T G G C F CC D CCP+ C+ C + S + S + ++ V C D T C TCC + +GCC AVCC D + CCP + C++ +C
Sbjct: 84 VSANLQMATKSFRMIRSYMGEDEDNICPD-QSRCPAEFSCLKA-LTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVK-----------------KESIKTVLCKDGVSECPDETTCCENQ-------------EGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVSVKMEDVPC-DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC 426 HSP 5 Score: 106.686 bits (265), Expect = 3.570e-24 Identity = 93/350 (26.57%), Postives = 138/350 (39.43%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIP-----ALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCT------------------------LNSTDFDTEKSKHSPT-------------TNRLSTVVCPDNETECFSWETCCAVSD-KSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
+ T C ++ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P ++K+ED P + + D+ C C T+ + CCP AVCC D CCP+ TC++ + C S+ ++ + K+ + C +G G C CC D CCP T CD C L T +TE+ ++ P T+ + C D+ T+C TCC + + +GCC L AVCCAD CCP + KC + SCV+
Sbjct: 613 DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFSNVSVKMEDVP----CDGSTSCPDQTTC-------------CKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV-----PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISC-DSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVK 938 HSP 6 Score: 95.9005 bits (237), Expect = 1.270e-20 Identity = 61/222 (27.48%), Postives = 98/222 (44.14%), Query Frame = 3
Query: 564 NYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLG-------FKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+Y CCP A CC D CCP+ CD+ C ++ S+ + E + + + T F++I +G Q ++F C R G C + G C D + CCP+ +C + C + + TV+C D +EC TCC + + CC + AVCC D++ CCP CD++ C+ ++S+K FP R++
Sbjct: 39 DYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWV--ERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSCLKALTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVKKES---------------IKTVLCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCL-SSSDKALPMWAKFPARTR 242 HSP 7 Score: 93.5893 bits (231), Expect = 6.134e-20 Identity = 89/370 (24.05%), Postives = 133/370 (35.95%), Query Frame = 3
Query: 180 WREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNR-----------LSTVVCPDNETE------------------------------------CFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
W + S ++CPN KC + QTCCQ+ Y CC + A CC D HCCP+ CD C S + ++ L++ + ++ + + E ++ +CP+ + Y CCP V C D CCPE C + ++ C K E+ K + G + ++ C G+ C CC D CCP+ T CD + KC L+S+D P R +S V NET+ C TCC + +GCC AVCC D + CCP + C++ +C
Sbjct: 4 WVVICWVSLALVAADMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATK-SFRMIRSYMGEDEDNICPDQSRCPAEFSCLKAL--TRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC------------------VKKES--IKTVLCKDGVSECPDET-TCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKC-LSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC 347 HSP 8 Score: 68.1662 bits (165), Expect = 8.534e-12 Identity = 67/243 (27.57%), Postives = 99/243 (40.74%), Query Frame = 3
Query: 219 GNICPNKRTKCTERQTCCQTKH-NSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKY----ETNFFKIISLLLGFKQKAS--KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS 926
G I + T+C + TCC K + CC AVCC D HCCP C + CVK + + IPA + + + V++ E M +CC + CCP NAVCC DK CCPE TCD+ +K C H + + E + + + ++ + +L S + C ++ G C CC D++ CCP +C K C N+
Sbjct: 880 GIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVK-GEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHM------------SCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSC----HKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCIKNT 1104
BLAST of Granulin b vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 117.857 bits (294), Expect = 7.877e-28 Identity = 87/326 (26.69%), Postives = 134/326 (41.10%), Query Frame = 3
Query: 177 AWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN--STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
A+ E + + + +I N+ C ++ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V CP+ TCC T+ + CCP AVCC D CCP+ TC++ + C S+ ++ + K+ + C G G C F CC D CCP+ C+ C + S + S + ++ V C D T C TCC + +GCC AVCC D + CCP + C++ +C
Sbjct: 278 AFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV-----PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPC-DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC 584 HSP 2 Score: 115.161 bits (287), Expect = 5.076e-27 Identity = 84/312 (26.92%), Postives = 128/312 (41.03%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA-----LKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN--STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
+ T C ++ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ +K+ED P + + D+ C C T+ + CCP AVCC D CCP+ TC++ + C S+ ++ + K+ + C G G C F CC D CCP+ C+ C + S + S + ++ V C D T C TCC + +GCC AVCC D + CCP + C++ +C
Sbjct: 376 DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFSNVSVKMEDVP----CDGSTSCPDQTTC-------------CKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV-----PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPC-DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC 663 HSP 3 Score: 114.39 bits (285), Expect = 9.821e-27 Identity = 95/360 (26.39%), Postives = 145/360 (40.28%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNIL------------------------EAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKC-----------TLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+ T C ++ TCC+TK ++ CC AVCC D HCCP G+TCD C+K A V M K +PA + ++ E K +E+ + C ++ + T C + + + CCP AVCC+D CCP C ++ C I + DS + +++F + ++ + + C + G G C CC D CCP+ CD + C L ++S+H P L+ D+ C ETCC S ++GCC AVCC D CCP +C K SC++ ++ F + K
Sbjct: 692 DGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLK-ASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAV-----HQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRC---DDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCIKNTGLHWLNWHTFFTNKRK 1041 HSP 4 Score: 109.768 bits (273), Expect = 3.116e-25 Identity = 102/376 (27.13%), Postives = 140/376 (37.23%), Query Frame = 3
Query: 162 ISFQLAWREQGFASNKETVG----NICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNE-KDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDS-------------------------FKYETNFFKIISLLLG--FKQKASKSDFYCPIT-------------NGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN--STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
+S L + F + +G NICP+ +++C +C + Y CC A V CPD HCCPEG C CVK ++ KT + + V+E DE C N+ + CCP AVCC DK CCPE TTCD++ KC SS M + + F T + + L G +AS D C T G G C F CC D CCP+ C+ C + S + S + ++ V C D T C TCC + +GCC AVCC D + CCP + C++ +C
Sbjct: 84 VSANLQMATKSFRMIRSYMGEDEDNICPD-QSRCPAEFSCLKA-LTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVK-----------------KESIKTVLCKDGVSECPDETTCCENQ-------------EGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVSVKMEDVPC-DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC 426 HSP 5 Score: 105.916 bits (263), Expect = 6.192e-24 Identity = 97/357 (27.17%), Postives = 140/357 (39.22%), Query Frame = 3
Query: 234 NKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQ-----------KASKSDF------------------YCPITNG--------RSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDT-EKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLT 1190
+ T C ++ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V CP+ TCC TK + CCP AVCC D CCP TTCD+ C + + M+++L T S L+ Q + + DF CP + G C CC+D CCP KC E++ C K + + S+ V +C +CC + +GCC L NAVCC D+ CCP CD+ + SC + + + PLT
Sbjct: 613 DGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQ-------TTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKA-SGLVPMSKKL-----PASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLT 950 HSP 6 Score: 95.9005 bits (237), Expect = 1.386e-20 Identity = 61/222 (27.48%), Postives = 98/222 (44.14%), Query Frame = 3
Query: 564 NYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLG-------FKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSK 1208
+Y CCP A CC D CCP+ CD+ C ++ S+ + E + + + T F++I +G Q ++F C R G C + G C D + CCP+ +C + C + + TV+C D +EC TCC + + CC + AVCC D++ CCP CD++ C+ ++S+K FP R++
Sbjct: 39 DYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWV--ERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSCLKALTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVKKES---------------IKTVLCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCL-SSSDKALPMWAKFPARTR 242 HSP 7 Score: 93.5893 bits (231), Expect = 7.067e-20 Identity = 89/370 (24.05%), Postives = 133/370 (35.95%), Query Frame = 3
Query: 180 WREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNR-----------LSTVVCPDNETE------------------------------------CFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC 1148
W + S ++CPN KC + QTCCQ+ Y CC + A CC D HCCP+ CD C S + ++ L++ + ++ + + E ++ +CP+ + Y CCP V C D CCPE C + ++ C K E+ K + G + ++ C G+ C CC D CCP+ T CD + KC L+S+D P R +S V NET+ C TCC + +GCC AVCC D + CCP + C++ +C
Sbjct: 4 WVVICWVSLALVAADMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATK-SFRMIRSYMGEDEDNICPDQSRCPAEFSCLKAL--TRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC------------------VKKES--IKTVLCKDGVSECPDET-TCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKC-LSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTC 347 HSP 8 Score: 68.1662 bits (165), Expect = 9.439e-12 Identity = 67/243 (27.57%), Postives = 99/243 (40.74%), Query Frame = 3
Query: 219 GNICPNKRTKCTERQTCCQTKH-NSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKY----ETNFFKIISLLLGFKQKAS--KSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS 926
G I + T+C + TCC K + CC AVCC D HCCP C + CVK + + IPA + + + V++ E M +CC + CCP NAVCC DK CCPE TCD+ +K C H + + E + + + ++ + +L S + C ++ G C CC D++ CCP +C K C N+
Sbjct: 801 GIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVK-GEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHM------------SCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSC----HKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCIKNT 1025
BLAST of Granulin b vs. Ensembl Medaka
Match: ENSORLT00000029765.1 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 74.7146 bits (182), Expect = 1.407e-14 Identity = 72/270 (26.67%), Postives = 102/270 (37.78%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLI-------PALKVEDEPKTNILEAKVNEKDEV------------MCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSP--------HSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASK-------SDFYCPITN----GRSGR---CKFSAGTCCSDLRTCCPQHTKCDEKNNKC 914
CP+ + C + TCC T H + CC AVCC DK HCCP G C+ C K + V + L PAL + TN++E N+ MCP+ T C+ ++CCP+ CC D + CCP CD+ C ++ + LI + K ++ L S+ S F+CP G G+ C + G CC+D R CC CD + C
Sbjct: 24 CPDGKL-CPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDL----STNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHP-------KGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 280
BLAST of Granulin b vs. Ensembl Medaka
Match: ENSORLT00000005071.2 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 74.7146 bits (182), Expect = 1.574e-14 Identity = 72/270 (26.67%), Postives = 102/270 (37.78%), Query Frame = 3
Query: 228 CPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLI-------PALKVEDEPKTNILEAKVNEKDEV------------MCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSP--------HSIHEMAQELIHDSFKYETNFFKIISLLLGFKQKASK-------SDFYCPITN----GRSGR---CKFSAGTCCSDLRTCCPQHTKCDEKNNKC 914
CP+ + C + TCC T H + CC AVCC DK HCCP G C+ C K + V + L PAL + TN++E N+ MCP+ T C+ ++CCP+ CC D + CCP CD+ C ++ + LI + K ++ L S+ S F+CP G G+ C + G CC+D R CC CD + C
Sbjct: 26 CPDGKL-CPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLS----TNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHP-------KGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 282
BLAST of Granulin b vs. Planmine SMEST
Match: SMESG000018760.1 (SMESG000018760.1) HSP 1 Score: 630.943 bits (1626), Expect = 0.000e+0 Identity = 332/352 (94.32%), Postives = 335/352 (95.17%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHE----MAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL 1250
K CPN RTKC + QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVE+E KTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHE MAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSP TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL
Sbjct: 284 KIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEIAHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPNTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL 635 HSP 2 Score: 263.077 bits (671), Expect = 4.239e-81 Identity = 172/343 (50.15%), Postives = 207/343 (60.35%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDE------VMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQ---ELIHDSFKYETNFFKIIS--LLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTT---NRLST------------VVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVET 1157
K CPN RTKC + QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM KLIPALKVEDEPKTNILEAKVNEKDE +CPN +TKC + +TCC TKHN+Y+CC + NAVCC DK+ CCP T+CD +KKC + + Q E + ET+ KII+ + K S C +G CKF+ CC D CCP+ + CD K +KC + + E K P N L T V+CP+N+T+C +TCC +Y CC +NAVCC+D+ CCP CDIK C +
Sbjct: 98 KIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDENKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETD-VKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKC-VKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS 438 HSP 3 Score: 214.157 bits (544), Expect = 1.340e-62 Identity = 147/343 (42.86%), Postives = 186/343 (54.23%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYE--TNFFK------------IISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN-----STDFDTEKSKHSPTTNR-----LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
A NKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCP G++CD+ KC + N+E K E I E V + CPNN+TKC+D++TCC+TKH +Y+CC NAVCC DK+ CCPE +TCD K KC S EM + + + E TN + + ++ + K ++ C + CK++ CC D CCP T CD + KC N S T + P ++ CP+N T+C +TCC SY CC+ NAVCC D+ CCP CD K + CV+
Sbjct: 7 APNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNR----NIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDENKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVK 345
BLAST of Granulin b vs. Planmine SMEST
Match: SMESG000018760.1 (SMESG000018760.1) HSP 1 Score: 630.558 bits (1625), Expect = 0.000e+0 Identity = 332/352 (94.32%), Postives = 335/352 (95.17%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHE----MAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL 1250
K CPN RTKC + QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVE+E KTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHE MAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSP TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL
Sbjct: 315 KIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEIAHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPNTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL 666 HSP 2 Score: 268.855 bits (686), Expect = 6.631e-83 Identity = 185/374 (49.47%), Postives = 213/374 (56.95%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHK-------------------------------------NAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQ---ELIHDSFKYETNFFKIIS--LLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTT---NRLST------------VVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVET 1157
K CPN RTKC + QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM KLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKD KTCCITKHNNYYCCPHK NAVCC DK+ CCP T+CD +KKC + + Q E + ET+ KII+ + K S C +G CKF+ CC D CCP+ + CD K +KC + + E K P N L T V+CP+N+T+C +TCC +Y CC +NAVCC+D+ CCP CDIK C +
Sbjct: 98 KIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDIKTCCITKHNNYYCCPHKNNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETD-VKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKC-VKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS 469 HSP 3 Score: 202.986 bits (515), Expect = 3.162e-58 Identity = 147/374 (39.30%), Postives = 187/374 (50.00%), Query Frame = 3
Query: 198 ASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL----IHDSFK-----------------------------------------YETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN-----STDFDTEKSKHSPTTNR-----LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
A NKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCP G++CD+ KC + N+E K E I E V + CPNN+TKC+D++TCC+TKH +Y+CC NAVCC DK+ CCPE +TCD K KC S EM + + + D K ++ N + ++ + K ++ C + CK++ CC D CCP T CD + KC N S T + P ++ CP+N T+C +TCC SY CC+ NAVCC D+ CCP CD K + CV+
Sbjct: 7 APNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNR----NIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDIKTCCITKHNNYYCCPHKNNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVK 376
BLAST of Granulin b vs. Planmine SMEST
Match: SMESG000018760.1 (SMESG000018760.1) HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0 Identity = 332/352 (94.32%), Postives = 335/352 (95.17%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHE----MAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL 1250
K CPN RTKC + QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVE+E KTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHE MAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSP TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL
Sbjct: 344 KIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEIAHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPNTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNHFSIL 695 HSP 2 Score: 268.47 bits (685), Expect = 1.510e-82 Identity = 185/374 (49.47%), Postives = 213/374 (56.95%), Query Frame = 3
Query: 207 KETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHK-------------------------------------NAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQ---ELIHDSFKYETNFFKIIS--LLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPTT---NRLST------------VVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVET 1157
K CPN RTKC + QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM KLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKD KTCCITKHNNYYCCPHK NAVCC DK+ CCP T+CD +KKC + + Q E + ET+ KII+ + K S C +G CKF+ CC D CCP+ + CD K +KC + + E K P N L T V+CP+N+T+C +TCC +Y CC +NAVCC+D+ CCP CDIK C +
Sbjct: 127 KIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDIKTCCITKHNNYYCCPHKNNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETD-VKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKC-VKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSS 498 HSP 3 Score: 226.868 bits (577), Expect = 6.202e-67 Identity = 159/412 (38.59%), Postives = 206/412 (50.00%), Query Frame = 3
Query: 84 MKYSYSIFAVLVGLAVIGSKVAAKGVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL----IHDSFK-----------------------------------------YETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN-----STDFDTEKSKHSPTTNR-----LSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
MK +SI + V L ++ S A G +SF L+W + NKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCP G++CD+ KC + N+E K E I E V + CPNN+TKC+D++TCC+TKH +Y+CC NAVCC DK+ CCPE +TCD K KC S EM + + + D K ++ N + ++ + K ++ C + CK++ CC D CCP T CD + KC N S T + P ++ CP+N T+C +TCC SY CC+ NAVCC D+ CCP CD K + CV+
Sbjct: 1 MKNIFSIVTICVVLTLVSSLAEAGGTLSFNLSWLDN---ENKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNR----NIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDIKTCCITKHNNYYCCPHKNNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVK 405 HSP 4 Score: 189.119 bits (479), Expect = 4.892e-53 Identity = 141/353 (39.94%), Postives = 189/353 (53.54%), Query Frame = 3
Query: 201 SNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQEL--IHDSFKYETNFF------KIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN-----------STDFDTEKSKHSPTTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSC----VETNSNKRRHFPLT 1190
+NKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCP G++CD+ KC + N+E K E I E V + CPNN+TKC+D++TCC+TKH +Y+CC NAVCC DK+ CCPE +TCD K KC S EM + + + + +TN K + K K + C + C CCSD CCP+ T CD KN KCT + + + + K+ ++ +++ + S + C +++ G C+ CC+D +CCP + KCD K N C + ++ K +H P T
Sbjct: 254 NNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNR----NIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEIAHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKS-DFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPNT 601
BLAST of Granulin b vs. Planmine SMEST
Match: SMESG000018767.1 (SMESG000018767.1) HSP 1 Score: 319.701 bits (818), Expect = 1.948e-104 Identity = 200/392 (51.02%), Postives = 234/392 (59.69%), Query Frame = 3
Query: 156 GVISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEM--AQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKF-------------------------------------SAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD-------FDTEKSKHSPTTNRLST-------VVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
G S + + T NICPNKRTKCT+RQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPE T CD+KNKKC S+ + + H F E + ++++ L ++ + CP + G+ G CKF + CC D CCP+ + CD K +KC + K + P TN L V+CP+N+T+C +TCC +Y CC +NAVCC+D+ CCP KCD+K C+
Sbjct: 5 GSKSLHASKTLEDMLKKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCL 396 HSP 2 Score: 307.76 bits (787), Expect = 9.381e-100 Identity = 173/252 (68.65%), Postives = 193/252 (76.59%), Query Frame = 3
Query: 195 FASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEM--AQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN 923
F T NICPNKRTKCT+RQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPE T CD+KNKKC S+ + + H F E + ++++ L ++ + CP + G+ G CKF+ G CC DL+TCCP T+CD+K N+C N
Sbjct: 240 FNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCIAN 491 HSP 3 Score: 153.68 bits (387), Expect = 2.688e-41 Identity = 132/372 (35.48%), Postives = 180/372 (48.39%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCA--------------------KSNVEMGKLIPALKVE----DEPKTN-------ILEA------KVNEKD---EVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYE--TNFF------KIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS------TDFDT--------EKSKHSPTTNRLST-VVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
+CPN +TKC + +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+ CD K KC+ + +SN E+ + K+E D+ T +++A K N K+ + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPE +TCD K KC S EM + + + E TN K + K K + C + C CCSD CCP+ TKCD KN KC S T+ DT E+S + C D + C + CC + +GCC+ + VCC D +CCPH +CD KTN C+
Sbjct: 120 MCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYEL--VFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCI 489 HSP 4 Score: 96.6709 bits (239), Expect = 2.139e-21 Identity = 94/286 (32.87%), Postives = 122/286 (42.66%), Query Frame = 3
Query: 477 KVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYE--TNFF------KIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS------TDFDT--------EKSKHSPT--------------------------------------TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
K N + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPE +TCD K KC S EM + + + E TN K + K K + C + C CCSD CCP+ TKCD KN KC S T+ DT E+S + N + +CP+ T+C +TCC SY CC+ NAVCC D+ CCP CD K + CV+
Sbjct: 20 KKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVK 305
BLAST of Granulin b vs. Planmine SMEST
Match: SMESG000018762.1 (SMESG000018762.1) HSP 1 Score: 319.316 bits (817), Expect = 1.795e-103 Identity = 209/415 (50.36%), Postives = 246/415 (59.28%), Query Frame = 3
Query: 99 SIFAVLVGLAVIGSKVAA---KGV-ISFQLAWREQGFASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEM--AQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKF-------------------------------------SAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTD-------FDTEKSKHSPTTNRLST-------VVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
S+ A L LA S +A +GV +L + N T NICPNKRTKCT+RQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPE T CD+KNKKC S+ + + H F E + ++++ L ++ + CP + G+ G CKF + CC D CCP+ + CD K +KC + K + P TN L V+CP+N+T+C +TCC +Y CC +NAVCC+D+ CCP KCD+K C+
Sbjct: 50 SLLAPLYALAP-DSLIALPDYRGVKTPDKLELISLEYLKN-VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCL 462 HSP 2 Score: 308.145 bits (788), Expect = 3.606e-99 Identity = 173/252 (68.65%), Postives = 193/252 (76.59%), Query Frame = 3
Query: 195 FASNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEM--AQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCP-------ITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN 923
F T NICPNKRTKCT+RQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPE T CD+KNKKC S+ + + H F E + ++++ L ++ + CP + G+ G CKF+ G CC DL+TCCP T+CD+K N+C N
Sbjct: 306 FNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCIAN 557 HSP 3 Score: 153.68 bits (387), Expect = 7.259e-41 Identity = 132/372 (35.48%), Postives = 180/372 (48.39%), Query Frame = 3
Query: 225 ICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCA--------------------KSNVEMGKLIPALKVE----DEPKTN-------ILEA------KVNEKD---EVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYE--TNFF------KIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS------TDFDT--------EKSKHSPTTNRLST-VVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCV 1151
+CPN +TKC + +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+ CD K KC+ + +SN E+ + K+E D+ T +++A K N K+ + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPE +TCD K KC S EM + + + E TN K + K K + C + C CCSD CCP+ TKCD KN KC S T+ DT E+S + C D + C + CC + +GCC+ + VCC D +CCPH +CD KTN C+
Sbjct: 186 MCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYEL--VFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCI 555 HSP 4 Score: 99.3673 bits (246), Expect = 3.710e-22 Identity = 97/301 (32.23%), Postives = 128/301 (42.52%), Query Frame = 3
Query: 432 ALKVEDEPKTNILEAKVNEKDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEMAQELIHDSFKYE--TNFF------KIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNS------TDFDT--------EKSKHSPT--------------------------------------TNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVE 1154
+K D+ + LE N + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPE +TCD K KC S EM + + + E TN K + K K + C + C CCSD CCP+ TKCD KN KC S T+ DT E+S + N + +CP+ T+C +TCC SY CC+ NAVCC D+ CCP CD K + CV+
Sbjct: 71 GVKTPDKLELISLEYLKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVK 371 The following BLAST results are available for this feature:
BLAST of Granulin b vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Granulin b vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of Granulin b vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Granulin b vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Granulin b vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Granulin b vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Granulin b vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Granulin b vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Granulin b vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Granulin b vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Granulin b vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Granulin b vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Granulin b vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Granulin b vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003962 ID=SMED30003962|Name=Granulin b|organism=Schmidtea mediterranea sexual|type=transcript|length=1344bpback to top protein sequence of SMED30003962-orf-1 >SMED30003962-orf-1 ID=SMED30003962-orf-1|Name=SMED30003962-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=390bp MKYSYSIFAVLVGLAVIGSKVAAKGVISFQLAWREQGFASNKETVGNICPback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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