Sodium leak channel non-selective protein

Overview
NameSodium leak channel non-selective protein
Smed IDSMED30003934
Length (bp)571
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Sodium leak channel non-selective protein (SMED30003934) t-SNE clustered cells

Violin plots show distribution of expression levels for Sodium leak channel non-selective protein (SMED30003934) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Sodium leak channel non-selective protein (SMED30003934) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Sodium leak channel non-selective protein (SMED30003934) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003934

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30003934SMESG000076454.1 SMESG000076415.1 dd_Smed_v4_7314_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30003934SMESG000076454.1 SMESG000076415.1 dd_Smed_v6_7314_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Human
Match: NALCN (sodium leak channel, non-selective [Source:HGNC Symbol;Acc:HGNC:19082])

HSP 1 Score: 62.7734 bits (151), Expect = 1.055e-11
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Human
Match: NALCN (sodium leak channel, non-selective [Source:HGNC Symbol;Acc:HGNC:19082])

HSP 1 Score: 62.3882 bits (150), Expect = 4.421e-11
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Human
Match: NALCN (sodium leak channel, non-selective [Source:HGNC Symbol;Acc:HGNC:19082])

HSP 1 Score: 62.3882 bits (150), Expect = 5.454e-11
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Fly
Match: na (gene:FBgn0002917 transcript:FBtr0344822)

HSP 1 Score: 56.225 bits (134), Expect = 3.430e-9
Identity = 35/81 (43.21%), Postives = 51/81 (62.96%), Query Frame = 2 
Query:  227 GVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGII 469
            G E + +++ D   + WV   W R   ++ AL+SL SVS NTP+TF+ Y  L ++T+  D  V LLFTAEM  KM I+G++
Sbjct:  339 GPEESLNESAD---IEWVNKLWVRRLMRLCALVSLTSVSLNTPKTFERYPSLQFITFASDTAVTLLFTAEMIAKMHIRGVL 416
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Fly
Match: na (gene:FBgn0002917 transcript:FBtr0344823)

HSP 1 Score: 56.225 bits (134), Expect = 3.462e-9
Identity = 35/81 (43.21%), Postives = 51/81 (62.96%), Query Frame = 2 
Query:  227 GVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGII 469
            G E + +++ D   + WV   W R   ++ AL+SL SVS NTP+TF+ Y  L ++T+  D  V LLFTAEM  KM I+G++
Sbjct:  339 GPEESLNESAD---IEWVNKLWVRRLMRLCALVSLTSVSLNTPKTFERYPSLQFITFASDTAVTLLFTAEMIAKMHIRGVL 416
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Zebrafish
Match: nalcn (sodium leak channel, non-selective [Source:ZFIN;Acc:ZDB-GENE-050410-12])

HSP 1 Score: 60.8474 bits (146), Expect = 1.204e-10
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:   28 KRKQSSRVEAQPM---TDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIK 122
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Zebrafish
Match: nalcn (sodium leak channel, non-selective [Source:ZFIN;Acc:ZDB-GENE-050410-12])

HSP 1 Score: 60.8474 bits (146), Expect = 1.285e-10
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:    3 KRKQSSRVEAQPM---TDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Zebrafish
Match: nalcn (sodium leak channel, non-selective [Source:ZFIN;Acc:ZDB-GENE-050410-12])

HSP 1 Score: 60.077 bits (144), Expect = 1.445e-10
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:   28 KRKQSSRVEAQPM---TDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIK 122
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Zebrafish
Match: nalcn (sodium leak channel, non-selective [Source:ZFIN;Acc:ZDB-GENE-050410-12])

HSP 1 Score: 60.077 bits (144), Expect = 1.692e-10
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:    3 KRKQSSRVEAQPM---TDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIIK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Xenopus
Match: NALCN (sodium leak channel, non selective [Source:NCBI gene;Acc:100492286])

HSP 1 Score: 63.5438 bits (153), Expect = 2.002e-11
Identity = 38/98 (38.78%), Postives = 57/98 (58.16%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Xenopus
Match: NALCN (sodium leak channel, non selective [Source:NCBI gene;Acc:100492286])

HSP 1 Score: 63.5438 bits (153), Expect = 2.123e-11
Identity = 38/98 (38.78%), Postives = 57/98 (58.16%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Xenopus
Match: NALCN (sodium leak channel, non selective [Source:NCBI gene;Acc:100492286])

HSP 1 Score: 63.1586 bits (152), Expect = 2.205e-11
Identity = 38/98 (38.78%), Postives = 57/98 (58.16%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:   11 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPKTFEHYPPLQYVTFALDTLLMFLYTAEMIAKMHIRGIVK 105
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Mouse
Match: Nalcn (sodium leak channel, non-selective [Source:MGI Symbol;Acc:MGI:2444306])

HSP 1 Score: 62.3882 bits (150), Expect = 3.964e-11
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. UniProt/SwissProt
Match: sp|Q8IZF0|NALCN_HUMAN (Sodium leak channel non-selective protein OS=Homo sapiens OX=9606 GN=NALCN PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 2.619e-10
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. UniProt/SwissProt
Match: sp|Q8BXR5|NALCN_MOUSE (Sodium leak channel non-selective protein OS=Mus musculus OX=10090 GN=Nalcn PE=1 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 2.773e-10
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+ SV  NTP TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. UniProt/SwissProt
Match: sp|Q6Q760|NALCN_RAT (Sodium leak channel non-selective protein OS=Rattus norvegicus OX=10116 GN=Nalcn PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 3.866e-10
Identity = 37/98 (37.76%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D++  D   + W+   W     +I A++S+  V  NTP TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GI++
Sbjct:    3 KRKQSSRVEAQPV---TDFGPDESLSDNADILWINKPWVHSLLRICAIISVIPVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. TrEMBL
Match: A0A183QKS4 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 3.694e-26
Identity = 55/102 (53.92%), Postives = 75/102 (73.53%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRP 475
            M  SERK SH+R+TQ  + G++F+ DD  D TS  WVYT WA+   + AA++SLASVS NTP+TFK+   L Y+T++LD++VALLFT EM +KM I+G+  P
Sbjct:    1 MFLSERKASHSRDTQGNIVGIDFSGDDGSDSTSKEWVYTRWAKIMLRTAAMISLASVSMNTPDTFKQVPVLMYITFILDLMVALLFTVEMISKMYIEGLFVP 102
[Close]
BLAST of Sodium leak channel non-selective protein vs. TrEMBL
Match: A0A5K4EZ77 (Voltage-dependent calcium channel OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 8.796e-24
Identity = 55/102 (53.92%), Postives = 75/102 (73.53%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRP 475
            M  SERK SH+R+TQ  + G++F+ DD  D TS  WVYT WA+   + AA++SLASVS NTP+TFK+   L Y+T++LD++VALLFT EM +KM I+G+  P
Sbjct:    1 MFLSERKASHSRDTQGNIVGIDFSGDDGSDSTSKEWVYTRWAKIMLRTAAMISLASVSMNTPDTFKQVPVLMYITFILDLMVALLFTVEMISKMYIEGLFVP 102
[Close]
BLAST of Sodium leak channel non-selective protein vs. TrEMBL
Match: A0A430Q8H3 (Sodium leak channel non-selective protein OS=Schistosoma bovis OX=6184 GN=DC041_0001132 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 2.522e-23
Identity = 55/102 (53.92%), Postives = 76/102 (74.51%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRP 475
            M  SERK SH+R+TQ  + G++F+ DD  D TS  WVYT WA+  F+ AA++SLASVS NTP+TF++   L Y+T++LD++VALLFT EM +KM I+G+  P
Sbjct:    1 MFLSERKASHSRDTQGNIVGIDFSGDDGSDSTSKEWVYTRWAKIMFRTAAMISLASVSMNTPDTFRQVPVLMYITFILDLMVALLFTVEMISKMYIEGLFVP 102
[Close]
BLAST of Sodium leak channel non-selective protein vs. TrEMBL
Match: A0A3Q0KUE9 (Voltage-dependent calcium channel OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.426e-22
Identity = 55/102 (53.92%), Postives = 75/102 (73.53%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRP 475
            M  SERK SH+R+TQ  + G++F+ DD  D TS  WVYT WA+   + AA++SLASVS NTP+TFK+   L Y+T++LD++VALLFT EM +KM I+G+  P
Sbjct:    1 MFLSERKASHSRDTQGNIVGIDFSGDDGSDSTSKEWVYTRWAKIMLRTAAMISLASVSMNTPDTFKQVPVLMYITFILDLMVALLFTVEMISKMYIEGLFVP 102
[Close]
BLAST of Sodium leak channel non-selective protein vs. TrEMBL
Match: G4VG52 (Voltage-dependent calcium channel OS=Schistosoma mansoni OX=6183 GN=Smp_197640 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 2.497e-22
Identity = 55/102 (53.92%), Postives = 75/102 (73.53%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRP 475
            M  SERK SH+R+TQ  + G++F+ DD  D TS  WVYT WA+   + AA++SLASVS NTP+TFK+   L Y+T++LD++VALLFT EM +KM I+G+  P
Sbjct:    1 MFLSERKASHSRDTQGNIVGIDFSGDDGSDSTSKEWVYTRWAKIMLRTAAMISLASVSMNTPDTFKQVPVLMYITFILDLMVALLFTVEMISKMYIEGLFVP 102
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Cavefish
Match: nalcn (sodium leak channel, non-selective [Source:ZFIN;Acc:ZDB-GENE-050410-12])

HSP 1 Score: 60.8474 bits (146), Expect = 1.078e-10
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:    3 KRKQSSRVEAQPM---TDFGPDETLTDSADILWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLEYVTFALDTLLMFLYTAEMIAKMHIRGIIK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Nematostella
Match: EDO45175 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTZ8])

HSP 1 Score: 48.9062 bits (115), Expect = 3.763e-7
Identity = 29/69 (42.03%), Postives = 45/69 (65.22%), Query Frame = 2 
Query:  275 WVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRPVR 481
            W+ + W +   ++ ++LSL S++ NTPETF+    LFYVT LLDI +A +FT EM  +++  G I  +R
Sbjct:    7 WLKSWWFKRLIQVCSVLSLISMAMNTPETFQNIPSLFYVTLLLDIALATVFTVEMIAQIRKFGFIVELR 75
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Nematostella
Match: EDO45174 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTZ7])

HSP 1 Score: 47.7506 bits (112), Expect = 9.251e-7
Identity = 28/69 (40.58%), Postives = 45/69 (65.22%), Query Frame = 2 
Query:  275 WVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRPVR 481
            W+ + W +   ++ ++LSL S++ NTPETF+    LFYVT +LDI +A +FT EM  +++  G I  +R
Sbjct:    3 WLKSWWFKRLIQVCSVLSLMSMAMNTPETFRNVPSLFYVTLVLDITLATVFTVEMIAQIRKFGFIVDLR 71
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Medaka
Match: nalcn (sodium leak channel, non-selective [Source:NCBI gene;Acc:101158897])

HSP 1 Score: 62.003 bits (149), Expect = 3.917e-11
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:    3 KRKQSSRVEAQPM---TDFGPDETLADSADIFWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIIK 97
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Medaka
Match: nalcn (sodium leak channel, non-selective [Source:NCBI gene;Acc:101158897])

HSP 1 Score: 61.6178 bits (148), Expect = 5.266e-11
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:   23 KRKQSSRVEAQPM---TDFGPDETLADSADIFWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIIK 117
[Close]
BLAST of Sodium leak channel non-selective protein vs. Ensembl Medaka
Match: nalcn (sodium leak channel, non-selective [Source:NCBI gene;Acc:101158897])

HSP 1 Score: 61.6178 bits (148), Expect = 5.368e-11
Identity = 38/98 (38.78%), Postives = 55/98 (56.12%), Query Frame = 2 
Query:  182 ERKTSHTRETQPTLPGVEFATDDA-GDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIR 472
            +RK S   E QP     +F  D+   D   + W+   W     +  A++S+ SV  NTP+TF+ Y  L YVT+ LD ++  L+TAEM  KM I+GII+
Sbjct:   28 KRKQSSRVEAQPM---TDFGPDETLADSADIFWINKPWVHSLLRACAIISVISVCMNTPKTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIIK 122
[Close]
BLAST of Sodium leak channel non-selective protein vs. Planmine SMEST
Match: SMESG000076415.1 (SMESG000076415.1)

HSP 1 Score: 192.971 bits (489), Expect = 8.392e-57
Identity = 102/103 (99.03%), Postives = 103/103 (100.00%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRPV 478
            MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDI+VALLFTAEMFTKMKIKGIIRPV
Sbjct:    1 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDIVVALLFTAEMFTKMKIKGIIRPV 103
[Close]
BLAST of Sodium leak channel non-selective protein vs. Planmine SMEST
Match: SMESG000076415.1 (SMESG000076415.1)

HSP 1 Score: 192.971 bits (489), Expect = 8.811e-57
Identity = 102/103 (99.03%), Postives = 103/103 (100.00%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRPV 478
            MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDI+VALLFTAEMFTKMKIKGIIRPV
Sbjct:    1 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDIVVALLFTAEMFTKMKIKGIIRPV 103
[Close]
BLAST of Sodium leak channel non-selective protein vs. Planmine SMEST
Match: SMESG000076415.1 (SMESG000076415.1)

HSP 1 Score: 192.971 bits (489), Expect = 8.918e-57
Identity = 102/103 (99.03%), Postives = 103/103 (100.00%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRPV 478
            MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDI+VALLFTAEMFTKMKIKGIIRPV
Sbjct:    1 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDIVVALLFTAEMFTKMKIKGIIRPV 103
[Close]
BLAST of Sodium leak channel non-selective protein vs. Planmine SMEST
Match: SMESG000076415.1 (SMESG000076415.1)

HSP 1 Score: 192.971 bits (489), Expect = 9.099e-57
Identity = 102/103 (99.03%), Postives = 103/103 (100.00%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRPV 478
            MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDI+VALLFTAEMFTKMKIKGIIRPV
Sbjct:    1 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDIVVALLFTAEMFTKMKIKGIIRPV 103
[Close]
BLAST of Sodium leak channel non-selective protein vs. Planmine SMEST
Match: SMESG000076415.1 (SMESG000076415.1)

HSP 1 Score: 192.2 bits (487), Expect = 1.606e-56
Identity = 104/116 (89.66%), Postives = 107/116 (92.24%), Query Frame = 2 
Query:  170 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIXXXXXXXXXXXNTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGIIRPVRIIQYYYLKFYKA 517
            MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDI+VALLFTAEMFTKMKIKGIIRP   +   +L F K 
Sbjct:    1 MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAALLSLASVSANTPETFKEYKWLFYVTYLLDIVVALLFTAEMFTKMKIKGIIRPSPYVIVRHLFFVKV 116
The following BLAST results are available for this feature:
BLAST of Sodium leak channel non-selective protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
NALCN1.055e-1138.78sodium leak channel, non-selective [Source:HGNC Sy... [more]
NALCN4.421e-1138.78sodium leak channel, non-selective [Source:HGNC Sy... [more]
NALCN5.454e-1138.78sodium leak channel, non-selective [Source:HGNC Sy... [more]
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
na3.430e-943.21gene:FBgn0002917 transcript:FBtr0344822[more]
na3.462e-943.21gene:FBgn0002917 transcript:FBtr0344823[more]
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
nalcn1.204e-1038.78sodium leak channel, non-selective [Source:ZFIN;Ac... [more]
nalcn1.285e-1038.78sodium leak channel, non-selective [Source:ZFIN;Ac... [more]
nalcn1.445e-1038.78sodium leak channel, non-selective [Source:ZFIN;Ac... [more]
nalcn1.692e-1038.78sodium leak channel, non-selective [Source:ZFIN;Ac... [more]
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
NALCN2.002e-1138.78sodium leak channel, non selective [Source:NCBI ge... [more]
NALCN2.123e-1138.78sodium leak channel, non selective [Source:NCBI ge... [more]
NALCN2.205e-1138.78sodium leak channel, non selective [Source:NCBI ge... [more]
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Nalcn3.964e-1138.78sodium leak channel, non-selective [Source:MGI Sym... [more]
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BLAST of Sodium leak channel non-selective protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q8IZF0|NALCN_HUMAN2.619e-1038.78Sodium leak channel non-selective protein OS=Homo ... [more]
sp|Q8BXR5|NALCN_MOUSE2.773e-1038.78Sodium leak channel non-selective protein OS=Mus m... [more]
sp|Q6Q760|NALCN_RAT3.866e-1037.76Sodium leak channel non-selective protein OS=Rattu... [more]
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BLAST of Sodium leak channel non-selective protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183QKS43.694e-2653.92Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A5K4EZ778.796e-2453.92Voltage-dependent calcium channel OS=Schistosoma m... [more]
A0A430Q8H32.522e-2353.92Sodium leak channel non-selective protein OS=Schis... [more]
A0A3Q0KUE92.426e-2253.92Voltage-dependent calcium channel OS=Schistosoma m... [more]
G4VG522.497e-2253.92Voltage-dependent calcium channel OS=Schistosoma m... [more]
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nalcn1.078e-1038.78sodium leak channel, non-selective [Source:ZFIN;Ac... [more]
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO451753.763e-742.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO451749.251e-740.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Sodium leak channel non-selective protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nalcn3.917e-1138.78sodium leak channel, non-selective [Source:NCBI ge... [more]
nalcn5.266e-1138.78sodium leak channel, non-selective [Source:NCBI ge... [more]
nalcn5.368e-1138.78sodium leak channel, non-selective [Source:NCBI ge... [more]
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BLAST of Sodium leak channel non-selective protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000076415.18.392e-5799.03SMESG000076415.1[more]
SMESG000076415.18.811e-5799.03SMESG000076415.1[more]
SMESG000076415.18.918e-5799.03SMESG000076415.1[more]
SMESG000076415.19.099e-5799.03SMESG000076415.1[more]
SMESG000076415.11.606e-5689.66SMESG000076415.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003934 ID=SMED30003934|Name=Sodium leak channel non-selective protein|organism=Schmidtea mediterranea sexual|type=transcript|length=571bp
CTGGATTATTTGTGATATTTTTTGAATTATAATTTACAATAAATTATTAT
CAATAAAGTTATATTTTTCTAACCTGTAACTATTTTGGCAGTTCACGATT
TTGTTAAAAGTGCTTAATTTATTTTGAATAATAAAAATTAATGATTAAAT
AACAATCATTCCGATTACTATGCATCGAAGTGAACGAAAAACTAGTCATA
CAAGAGAGACTCAACCAACTTTACCAGGTGTAGAATTTGCCACCGACGAT
GCGGGTGATTTGACATCAGTTAACTGGGTCTATACGACATGGGCAAGATG
TGCATTTAAAATTGCCGCATTGTTAAGTCTAGCTTCAGTCAGTGCCAATA
CACCAGAGACATTTAAAGAATACAAGTGGTTATTCTATGTCACTTATCTA
CTTGATATCATTGTGGCGCTGTTATTCACAGCAGAAATGTTTACCAAAAT
GAAAATAAAGGGCATCATTCGCCCGGTACGTATTATTCAATATTATTACC
TTAAATTTTATAAAGCTGTAGCGACAATGAATAAATTAGTAATAAATAAC
ATTTAGTAAGCAACTATCGGG
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protein sequence of SMED30003934-orf-1

>SMED30003934-orf-1 ID=SMED30003934-orf-1|Name=SMED30003934-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp
MHRSERKTSHTRETQPTLPGVEFATDDAGDLTSVNWVYTTWARCAFKIAA
LLSLASVSANTPETFKEYKWLFYVTYLLDIIVALLFTAEMFTKMKIKGII
RPVRIIQYYYLKFYKAVATMNKLVINNI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR028823NALCN
Vocabulary: molecular function
TermDefinition
GO:0005216ion channel activity
GO:0005261cation channel activity
Vocabulary: biological process
TermDefinition
GO:0006811ion transport
GO:0034220ion transmembrane transport
GO:0055085transmembrane transport
GO:0098655cation transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR46141:SF1SODIUM LEAK CHANNEL NON-SELECTIVE PROTEINcoord: 6..101
NoneNo IPR availableTMHMMTMhelixcoord: 70..92
IPR028823Sodium leak channel non-selective proteinPANTHERPTHR46141FAMILY NOT NAMEDcoord: 6..101