MFS domain-containing protein

Overview
NameMFS domain-containing protein
Smed IDSMED30003923
Length (bp)873
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MFS domain-containing protein (SMED30003923) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30003923) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003923

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30003923h1SMcG0004953 SmedASXL_078659SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30003923h1SMcG0004953 SmedASXL_070184SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30003923h1SMcG0004953 SmedASXL_069607SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30003923h1SMcG0004953 Contig48725newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003923h1SMcG0004953 Contig48725uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003923h1SMcG0004953 Contig13260newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003923h1SMcG0004953 Contig13260uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30003923h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30003923h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003923h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003923h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30003923h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: Y59A8B.21 (pep chromosome:WBcel235:V:17986260:17994546:1 gene:WBGene00013353.1 transcript:Y59A8B.21.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y59A8B.21)

HSP 1 Score: 116.316 bits (290), Expect = 2.026e-29
Identity = 75/217 (34.56%), Postives = 125/217 (57.60%), Query Frame = 1 
Query:   76 GGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSL-AVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKL 723
            G   I  ++GT +TFGNM PY+ASY++  T+ + V    M+WI +    F   F M +GGL      PR++ +IG ++++    L+ +AI+++     + YG +FG+G+G+ Y  A++T + W P   G+++G V AG+GL S IF  IQT  +NP+N+      T + YF  P +L  +P  F  + VIY I+Q + ++++ +P  +K    E  L
Sbjct:   30 GAVSIHLTIGTYHTFGNMLPYMASYMRNFTDPS-VRIEHMMWIPTFQGCFP--FAMVIGGLTSSTFSPRVSAFIGCFLVTSSVALSAYAIQHSFALFFIVYGLLFGLGSGIAYVTAVSTAINWAPDKIGVISGIVAAGFGLSSSIFAPIQTMIVNPLNLPA----TKDGYFLQPELLHRVPALFTKLAVIYGIMQAMALIVVCDPPFRKSRSTESIL 239
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 107.842 bits (268), Expect = 2.882e-26
Identity = 69/227 (30.40%), Postives = 128/227 (56.39%), Query Frame = 1 
Query:   76 GGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            GG +   + G +YT GN+ PY+ SY +++      T G ++W+ +L +      M +GGL+E+K G R    +GS I ++G   T+++I+++  A ++T G I  +G+ + YS  L T  +WFP + GL  G ++ GYG G+ I + +QT FINP++   N     + +FT   +L+ +P  F+++ + +++ Q IG++ +  P+    + + E L+   S I   +
Sbjct:   37 GGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRT-GQLIWLQTL-LSGLPMGMVIGGLVERKFGGRAGALLGSIIYTIGVGSTFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVND----QGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPE-GDIHVETENLIGTNSEIRVEQ 256
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 107.457 bits (267), Expect = 3.091e-26
Identity = 69/227 (30.40%), Postives = 128/227 (56.39%), Query Frame = 1 
Query:   76 GGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            GG +   + G +YT GN+ PY+ SY +++      T G ++W+ +L +      M +GGL+E+K G R    +GS I ++G   T+++I+++  A ++T G I  +G+ + YS  L T  +WFP + GL  G ++ GYG G+ I + +QT FINP++   N     + +FT   +L+ +P  F+++ + +++ Q IG++ +  P+    + + E L+   S I   +
Sbjct:   23 GGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRT-GQLIWLQTL-LSGLPMGMVIGGLVERKFGGRAGALLGSIIYTIGVGSTFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVND----QGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPE-GDIHVETENLIGTNSEIRVEQ 242
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693542:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 107.457 bits (267), Expect = 3.091e-26
Identity = 69/227 (30.40%), Postives = 128/227 (56.39%), Query Frame = 1 
Query:   76 GGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            GG +   + G +YT GN+ PY+ SY +++      T G ++W+ +L +      M +GGL+E+K G R    +GS I ++G   T+++I+++  A ++T G I  +G+ + YS  L T  +WFP + GL  G ++ GYG G+ I + +QT FINP++   N     + +FT   +L+ +P  F+++ + +++ Q IG++ +  P+    + + E L+   S I   +
Sbjct:   23 GGVMCMVTFGIVYTCGNLLPYLVSYFRWKMYPDMRT-GQLIWLQTL-LSGLPMGMVIGGLVERKFGGRAGALLGSIIYTIGVGSTFYSIQHSYAATLLTMGGIASVGSSIAYSSILPTAQRWFPDNVGLAGGIIIGGYGCGAFILSPLQTTFINPLDYRVND----QGFFTQEDLLERVPYVFIVMSIFFTVFQSIGLIFIGQPE-GDIHVETENLIGTNSEIRVEQ 242
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Match: slc16a6b (solute carrier family 16 member 6b [Source:ZFIN;Acc:ZDB-GENE-110208-3])

HSP 1 Score: 49.2914 bits (116), Expect = 2.864e-6
Identity = 37/125 (29.60%), Postives = 60/125 (48.00%), Query Frame = 1 
Query:   82 FLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHR 456
            F+  F+ G I TFG        +L+        TN  + W  S+ VF  AFT  L  LL  + G R    +G +++SLGT+ T  A+  ++  + +T G + G+G  LT+   +    ++F   R
Sbjct:   31 FVEVFTYGIIKTFGI-------FLQDLMTEFHETNSRVSWAVSICVFVMAFTAPLSTLLSNRFGHRPVVMVGGFLISLGTITT--ALTQSIDEMYITMGIVAGLGYCLTFLPTVTILSQYFSKRR 146
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8HYG0 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.762e-68
Identity = 119/235 (50.64%), Postives = 167/235 (71.06%), Query Frame = 1 
Query:   55 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTV-EKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEE---KLLDETSSIE 747
            KGI  V GGFLI F+LGT+YTFGNMSPY+ SY++  +++ W+   D  WIF L++F Q  +M+ GGL+EK+IG RLT  +GSWIMSLG +L+YFA+K +L  ++VTYG +FG+GTG+ Y+VA+A  M WFP  RGLV G VVAG+G GSL+F+  QT +INP NV  N T    E+YF  P ++D +P CFV +G+IY+++Q +GV LL  P   +  + +E   +  D+T++ E
Sbjct:    6 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQE 240
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A267H5U2 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g2 PE=4 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 2.108e-68
Identity = 119/235 (50.64%), Postives = 167/235 (71.06%), Query Frame = 1 
Query:   55 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTV-EKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEE---KLLDETSSIE 747
            KGI  V GGFLI F+LGT+YTFGNMSPY+ SY++  +++ W+   D  WIF L++F Q  +M+ GGL+EK+IG RLT  +GSWIMSLG +L+YFA+K +L  ++VTYG +FG+GTG+ Y+VA+A  M WFP  RGLV G VVAG+G GSL+F+  QT +INP NV  N T    E+YF  P ++D +P CFV +G+IY+++Q +GV LL  P   +  + +E   +  D+T++ E
Sbjct:    8 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQE 242
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8HL50 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 2.353e-68
Identity = 119/235 (50.64%), Postives = 167/235 (71.06%), Query Frame = 1 
Query:   55 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTV-EKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEE---KLLDETSSIE 747
            KGI  V GGFLI F+LGT+YTFGNMSPY+ SY++  +++ W+   D  WIF L++F Q  +M+ GGL+EK+IG RLT  +GSWIMSLG +L+YFA+K +L  ++VTYG +FG+GTG+ Y+VA+A  M WFP  RGLV G VVAG+G GSL+F+  QT +INP NV  N T    E+YF  P ++D +P CFV +G+IY+++Q +GV LL  P   +  + +E   +  D+T++ E
Sbjct:    6 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQE 240
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8H205 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 2.712e-66
Identity = 119/235 (50.64%), Postives = 167/235 (71.06%), Query Frame = 1 
Query:   55 KGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTV-EKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEE---KLLDETSSIE 747
            KGI  V GGFLI F+LGT+YTFGNMSPY+ SY++  +++ W+   D  WIF L++F Q  +M+ GGL+EK+IG RLT  +GSWIMSLG +L+YFA+K +L  ++VTYG +FG+GTG+ Y+VA+A  M WFP  RGLV G VVAG+G GSL+F+  QT +INP NV  N T    E+YF  P ++D +P CFV +G+IY+++Q +GV LL  P   +  + +E   +  D+T++ E
Sbjct:    6 KGIGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEERQELVGEAGDDTAAQE 240
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A267DK45 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g4 PE=4 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 4.953e-65
Identity = 113/221 (51.13%), Postives = 157/221 (71.04%), Query Frame = 1 
Query:   61 ISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTV-EKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEK 720
            I  V GGFLI F+LGT+YTFGNMSPY+ SY++  +++ W+   D  WIF L++F Q  +M+ GGL+EK+IG RLT  +GSWIMSLG +L+YFA+K +L  ++VTYG +FG+GTG+ Y+VA+A  M WFP  RGLV G VVAG+G GSL+F+  QT +INP NV  N T    E+YF  P ++D +P CFV +G+IY+++Q +GV LL  P   +  +   +
Sbjct:    1 IGAVIGGFLIHFTLGTLYTFGNMSPYVVSYMRLHSHSKWIGYPDATWIFGLSIFGQGASMAFGGLIEKRIGARLTALLGSWIMSLGVLLSYFAVKSHLGLLVVTYGLLFGVGTGIAYAVAIAASMSWFPSRRGLVGGIVVAGFGGGSLVFDQFQTLYINPDNVRANVTAGGNERYFDQPAIIDRVPSCFVFLGLIYALMQAVGVALLRRPTAAELEEVNRR 221
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Match: EDO48450 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIS4])

HSP 1 Score: 44.2838 bits (103), Expect = 9.481e-6
Identity = 30/131 (22.90%), Postives = 57/131 (43.51%), Query Frame = 1 
Query:   61 ISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLH 453
            I C      +  + G  +++G + P    Y K  +  T        W+ SLA+ S     S+ G++ +K G RLTC IG    ++G  ++    K +   +  +Y  +FG G    ++ +     ++F  +
Sbjct:    2 IVCCSSAVCVALATGVTFSYGVLLPVFMDYFKENSEKT-------AWVGSLAICSTFLFGSVAGMIVQKFGCRLTCVIGGLSCAIGLGVSSLVDKMDY--LFGSYSLLFGFGCSCLFNSSFVVVTRYFDRY 123
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Medaka
Match: slc16a6a (monocarboxylate transporter 7 [Source:NCBI gene;Acc:101155804])

HSP 1 Score: 51.6026 bits (122), Expect = 5.195e-7
Identity = 36/123 (29.27%), Postives = 59/123 (47.97%), Query Frame = 1 
Query:   88 IQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHR 456
            + F L  I+T+G +  +    L  +T     +N  + W+ S+AVF   F   L  ++  + G +    IG  ++  GT+ T FA   N   + +TYGF+ G+G  LTY   +    K+F   R
Sbjct:   31 VAFFLVEIFTYGTIKIFGIFLLDLKTEFKE-SNSRISWVVSIAVFVMTFNGPLASVMTNRFGYQFVVMIGGLLICCGTIATSFASSIN--QIYITYGFVAGLGYCLTYLPTVTILSKYFNRRR 150
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Medaka
Match: slc16a6a (monocarboxylate transporter 7 [Source:NCBI gene;Acc:101155804])

HSP 1 Score: 51.6026 bits (122), Expect = 5.195e-7
Identity = 36/123 (29.27%), Postives = 59/123 (47.97%), Query Frame = 1 
Query:   88 IQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHR 456
            + F L  I+T+G +  +    L  +T     +N  + W+ S+AVF   F   L  ++  + G +    IG  ++  GT+ T FA   N   + +TYGF+ G+G  LTY   +    K+F   R
Sbjct:   31 VAFFLVEIFTYGTIKIFGIFLLDLKTEFKE-SNSRISWVVSIAVFVMTFNGPLASVMTNRFGYQFVVMIGGLLICCGTIATSFASSIN--QIYITYGFVAGLGYCLTYLPTVTILSKYFNRRR 150
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 392.504 bits (1007), Expect = 1.833e-136
Identity = 209/210 (99.52%), Postives = 209/210 (99.52%), Query Frame = 1 
Query:  127 MSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            MSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSW MSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE
Sbjct:    1 MSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWTMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 210
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 345.895 bits (886), Expect = 1.975e-118
Identity = 187/188 (99.47%), Postives = 187/188 (99.47%), Query Frame = 1 
Query:  193 MVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            MVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSW MSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE
Sbjct:    1 MVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWTMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 188
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 317.005 bits (811), Expect = 2.848e-107
Identity = 172/173 (99.42%), Postives = 172/173 (99.42%), Query Frame = 1 
Query:  238 MSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            MSLGGLLEKKIGPRLTCWIGSW MSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE
Sbjct:    1 MSLGGLLEKKIGPRLTCWIGSWTMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 173
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060699.1 (SMESG000060699.1)

HSP 1 Score: 287.345 bits (734), Expect = 1.431e-94
Identity = 153/242 (63.22%), Postives = 187/242 (77.27%), Query Frame = 1 
Query:   31 MSETRNLLKGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            M    N  +GISCV GGF + FSLGT YTFGN+SPY+ASYLKYRT+NTWV +G MVW+F+ +       MSLGG+LEKK+G R+TC IG W MSLGT+LTY+A+K N+WAVIVTYG IFG G+GLTYSVA++  MKWFP  +G+VAGAV AGYGLGSLIFNI+QT++INP NVSPN T TVEKYFT+  VL+N+P CFV+IG  Y+IIQ IGV+LL++   KK    EEK L     + +NE
Sbjct:    1 MVRLGNTARGISCVTGGFFLHFSLGTFYTFGNISPYMASYLKYRTSNTWVNSGHMVWVFAFSALFLGLIMSLGGMLEKKVGARITCLIGGWSMSLGTILTYYAVKTNIWAVIVTYGVIFGGGSGLTYSVAISAAMKWFPTKKGMVAGAVAAGYGLGSLIFNIVQTKYINPFNVSPNKTSTVEKYFTNETVLNNVPFCFVIIGTAYAIIQLIGVILLMDSAFKK-PSREEKQLKNGERLLDNE 241
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060699.1 (SMESG000060699.1)

HSP 1 Score: 286.574 bits (732), Expect = 5.221e-94
Identity = 153/242 (63.22%), Postives = 187/242 (77.27%), Query Frame = 1 
Query:   31 MSETRNLLKGISCVCGGFLIQFSLGTIYTFGNMSPYIASYLKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGSWIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPLHRXXXXXXXXXXXXXXXXIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGVLDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEENE 756
            M    N  +GISCV GGF + FSLGT YTFGN+SPY+ASYLKYRT+NTWV +G MVW+F+ +       MSLGG+LEKK+G R+TC IG W MSLGT+LTY+A+K N+WAVIVTYG IFG G+GLTYSVA++  MKWFP  +G+VAGAV AGYGLGSLIFNI+QT++INP NVSPN T TVEKYFT+  VL+N+P CFV+IG  Y+IIQ IGV+LL++   KK     E  L   +S +ENE
Sbjct:    1 MVRLGNTARGISCVTGGFFLHFSLGTFYTFGNISPYMASYLKYRTSNTWVNSGHMVWVFAFSALFLGLIMSLGGMLEKKVGARITCLIGGWSMSLGTILTYYAVKTNIWAVIVTYGVIFGGGSGLTYSVAISAAMKWFPTKKGMVAGAVAAGYGLGSLIFNIVQTKYINPFNVSPNKTSTVEKYFTNETVLNNVPFCFVIIGTAYAIIQLIGVILLMDSAFKK--PSREVRLTLINSTKENE 240
The following BLAST results are available for this feature:
BLAST of MFS domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Y59A8B.212.026e-2934.56pep chromosome:WBcel235:V:17986260:17994546:1 gene... [more]
F10G7.52.882e-2630.40pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.53.091e-2630.40pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.53.091e-2630.40pep chromosome:WBcel235:II:4693542:4696408:1 gene:... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
slc16a6b2.864e-629.60solute carrier family 16 member 6b [Source:ZFIN;Ac... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HYG01.762e-6850.64MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A267H5U22.108e-6850.64MFS domain-containing protein (Fragment) OS=Macros... [more]
A0A1I8HL502.353e-6850.64MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A1I8H2052.712e-6650.64MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A267DK454.953e-6551.13MFS domain-containing protein (Fragment) OS=Macros... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO484509.481e-622.90Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
slc16a6a5.195e-729.27monocarboxylate transporter 7 [Source:NCBI gene;Ac... [more]
slc16a6a5.195e-729.27monocarboxylate transporter 7 [Source:NCBI gene;Ac... [more]
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000060691.11.833e-13699.52SMESG000060691.1[more]
SMESG000060691.11.975e-11899.47SMESG000060691.1[more]
SMESG000060691.12.848e-10799.42SMESG000060691.1[more]
SMESG000060699.11.431e-9463.22SMESG000060699.1[more]
SMESG000060699.15.221e-9463.22SMESG000060699.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003923 ID=SMED30003923|Name=MFS domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=873bp
CGGTCTTATCGAAATCTATATAAATCTTATATGAGTGAAACAAGGAACTT
ATTGAAAGGGATCTCATGCGTTTGCGGGGGATTCCTGATCCAGTTCTCCC
TAGGGACCATTTATACGTTTGGCAACATGAGCCCCTATATTGCCTCATAT
TTGAAATATCGCACTAACAATACGTGGGTAACCAACGGTGACATGGTATG
GATCTTCTCATTGGCTGTGTTTTCACAGGCTTTCACCATGTCTCTGGGAG
GACTTCTAGAAAAGAAAATAGGACCCCGATTGACATGCTGGATTGGTAGT
TGGATAATGAGCTTAGGCACAGTGCTGACTTATTTCGCTATCAAGTACAA
TTTGTGGGCCGTCATAGTCACTTATGGATTCATTTTTGGAATCGGAACCG
GACTGACATATTCCGTTGCTCTAGCAACTCCAATGAAGTGGTTTCCTCTT
CATCGAGGTCTTGTAGCAGGAGCAGTTGTTGCCGGATATGGACTAGGGTC
CCTGATATTCAATATCATACAGACTCAGTTCATAAATCCTATGAATGTCT
CTCCAAATTCAACTTTAACTGTTGAAAAATATTTTACTGATCCTGGAGTT
CTTGACAATATTCCTTTGTGTTTTGTACTTATTGGTGTCATTTATTCAAT
CATTCAATTTATTGGAGTTGTTTTATTAATAAATCCGGATGTCAAAAAAT
ATTATGATGATGAAGAAAAGTTACTGGACGAAACTTCAAGTATTGAAGAA
AATGAGTAAATTCTTTTTTTATTTACAGAGTTCATCTAATAACCAGACGA
AGAGGGGTAAATTATTTTGAAATTCTTAATGGTTTCATTATCTTTATACT
AATAGGGGGACAATTTACCTTGG
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protein sequence of SMED30003923-orf-1

>SMED30003923-orf-1 ID=SMED30003923-orf-1|Name=SMED30003923-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=253bp
RSYRNLYKSYMSETRNLLKGISCVCGGFLIQFSLGTIYTFGNMSPYIASY
LKYRTNNTWVTNGDMVWIFSLAVFSQAFTMSLGGLLEKKIGPRLTCWIGS
WIMSLGTVLTYFAIKYNLWAVIVTYGFIFGIGTGLTYSVALATPMKWFPL
HRGLVAGAVVAGYGLGSLIFNIIQTQFINPMNVSPNSTLTVEKYFTDPGV
LDNIPLCFVLIGVIYSIIQFIGVVLLINPDVKKYYDDEEKLLDETSSIEE
NE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 18..199
e-value: 1.2E-17
score: 63.9
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 3..199
e-value: 4.9E-17
score: 63.5
NoneNo IPR availablePANTHERPTHR11360:SF3OXALATE/FORMATE ANTIPORTERcoord: 15..187
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 15..187
NoneNo IPR availableCDDcd17353MFS_OFA_likecoord: 25..187
e-value: 4.92458E-33
score: 120.022
NoneNo IPR availableTMHMMTMhelixcoord: 77..99
NoneNo IPR availableTMHMMTMhelixcoord: 23..45
NoneNo IPR availableTMHMMTMhelixcoord: 162..184
NoneNo IPR availableTMHMMTMhelixcoord: 111..133
NoneNo IPR availableTMHMMTMhelixcoord: 52..72
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 1..210
score: 10.484
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 16..187