SMED30003912

Overview
NameSMED30003912
Smed IDSMED30003912
Length (bp)1218
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003912 (SMED30003912) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003912 (SMED30003912) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003912 (SMED30003912) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003912 (SMED30003912) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003912

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30003912SMESG000032328.1 Contig2482GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003912SMESG000001726.1 Contig2482GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30003912SMESG000001726.1 dd_Smed_v4_2615_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003912SMESG000001726.1 dd_Smed_v4_2615_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003912 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002980:7530..23765 -6020
Homology
BLAST of SMED30003912 vs. TrEMBL
Match: A0A1S3I5M6 (G-protein coupled receptor 157 OS=Lingula unguis OX=7574 GN=LOC106161225 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 7.456e-7
Identity = 79/321 (24.61%), Postives = 143/321 (44.55%), Query Frame = 2
Query:  221 FSCIFSVFSFLILLRMKVTDCTTGIRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYIYCQIQSFFSIWFNMIVSICILFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWI-SSCEQ---PKIRLIFMLLYSKXXXXXXXXXXXXXXXXXKYINYKHIKMERQALRIGISISTQSDVDSDKLD-IESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSQSSWTRNDFINFIENAILFIQAIFENLQGVFVFSVLILCSNFAKSYIR-RLFC 1165
             SCI S+   ++++   +      IR +S  +L+ L   D   +  NL G+IRY+     N+   +C     + C  QS  + + +M      + I ++    + G  G  R   +K      +++W +P +LT     +  LG+D ++T+ GWCWI ++C     P   + +MLL  K  EI+ +L+   +        Y HIK       + +       +DSD +  +E +  D KL F + I     R+WG IR F+ +G       +  +D    +   I+ +Q   ++ QG   F +  L ++  +  +R + FC
Sbjct:   42 LSCILSIIGSVLIIATYIA--FREIRSTSRKLLVYLSIVDFFTALGNLIGVIRYVYNYT-NEISTDCDNPGPV-CVAQSVITTFSSMASFFWTVMIALYLHVSVVG--GDSRKADRKVWAF-HVLSWGIPAMLTFTAWQYGVLGEDGSQTSAGWCWIKTTCSDGMSPGEEIGWMLLTGKAWEIIAYLLTPAI--------YGHIKCHIHRTIVQVH---HRYIDSDTIHAVERA--DRKLTF-VPIIFILLRIWGTIRFFLAIG------GYQPSD----MSALIIILQGFGDSGQGFCNFVLFCLTTDVIREKLREKPFC 331          
BLAST of SMED30003912 vs. Ensembl Cavefish
Match: gpr157 (G protein-coupled receptor 157 [Source:NCBI gene;Acc:103039591])

HSP 1 Score: 48.9062 bits (115), Expect = 5.450e-6
Identity = 79/324 (24.38%), Postives = 136/324 (41.98%), Query Frame = 2
Query:  218 LFSCIFSVFSFLILLRMKVTDCTTG----IRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYI--YCQIQSFFSIWFNMIVSICI-LFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQPKIRLIFMLLYSKXXXXXXXXXXXXXXXXXK-YINYKHIKMERQALRIGISISTQSDVDSDKLDIESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSQSSWTRNDFINFIENAILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC 1165
            L SC+FS    L+++      CT      +R +   +L+ L  SD L +     G+++     DF    R+C     I  +    SFF  W    V+I I L+I I    Q          +    ++   +++W VP  +T+      K+G D ++ + GWCW+S   Q    +++MLL  KI E L +L    L+I +K +I+  H  +      +  S ++QS          SS  D KL  LI I     R+W  +R  +    + + S   +N         ++ +  I    QG     + +LC+   ++ +  + C
Sbjct:   21 LLSCVFSFLGSLLII------CTYATWRDLRTTPRKLLVFLSVSDLLSALSYFYGVLK-----DFTSNTRDCIAQGAISTFANTSSFF--W---TVAIAIYLYILIVKASQ---------RLADSLVLYFHLISWGVPLAITVAALALQKIGYDASEVSVGWCWVSI--QQDDHVLWMLLTGKIWEFLAYLTLPVLYILIKIHIHKAHAALSEYRPILATSPTSQS---------MSSIADRKLT-LIPIIFIALRMWSTVRFLL----LLTGSPARQNP-------VLVILHGIGNTFQGAANCIMFVLCTRPVRARLIAVLC 296          
BLAST of SMED30003912 vs. Ensembl Cavefish
Match: gpr157 (G protein-coupled receptor 157 [Source:NCBI gene;Acc:103039591])

HSP 1 Score: 48.1358 bits (113), Expect = 9.377e-6
Identity = 79/324 (24.38%), Postives = 136/324 (41.98%), Query Frame = 2
Query:  218 LFSCIFSVFSFLILLRMKVTDCTTG----IRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYI--YCQIQSFFSIWFNMIVSICI-LFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQPKIRLIFMLLYSKXXXXXXXXXXXXXXXXXK-YINYKHIKMERQALRIGISISTQSDVDSDKLDIESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSQSSWTRNDFINFIENAILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC 1165
            L SC+FS    L+++      CT      +R +   +L+ L  SD L +     G+++     DF    R+C     I  +    SFF  W    V+I I L+I I    Q          +    ++   +++W VP  +T+      K+G D ++ + GWCW+S   Q    +++MLL  KI E L +L    L+I +K +I+  H  +      +  S ++QS          SS  D KL  LI I     R+W  +R  +    + + S   +N         ++ +  I    QG     + +LC+   ++ +  + C
Sbjct:   15 LLSCVFSFLGSLLII------CTYATWRDLRTTPRKLLVFLSVSDLLSALSYFYGVLK-----DFTSNTRDCIAQGAISTFANTSSFF--W---TVAIAIYLYILIVKASQ---------RLADSLVLYFHLISWGVPLAITVAALALQKIGYDASEVSVGWCWVS--IQQDDHVLWMLLTGKIWEFLAYLTLPVLYILIKIHIHKAHAALSEYRPILATSPTSQS---------MSSIADRKLT-LIPIIFIALRMWSTVRFLL----LLTGSPARQNP-------VLVILHGIGNTFQGAANCIMFVLCTRPVRARLIAVLC 290          
BLAST of SMED30003912 vs. Planmine SMEST
Match: SMESG000001726.1 (SMESG000001726.1)

HSP 1 Score: 641.728 bits (1654), Expect = 0.000e+0
Identity = 338/339 (99.71%), Postives = 339/339 (100.00%), Query Frame = 2
Query:  149 MSDDYSRDFKISQESTITAIVFGLFSCIFSVFSFLILLRMKVTDCTTGIRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYIYCQIQSFFSIWFNMIVSICILFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQPKIRLIFMLLYSKXXXXXXXXXXXXXXXXXKYINYKHIKMERQALRIGISISTQSDVDSDKLDIESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSQSSWTRNDFINFIENAILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC 1165
            MSDDYSRDFKISQESTITAIVFGLFSCIFSVFSFLILLRMKVTDCTTGIRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYIYCQIQSFFSIWFNMIVSICILFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQPKIRLIFMLLYSKILEILIFLINLTLFIFLKYINYKHIKMERQALRIGISISTQSDVDSDKLDIESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFS+SSWTRNDFINFIENAILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC
Sbjct:    1 MSDDYSRDFKISQESTITAIVFGLFSCIFSVFSFLILLRMKVTDCTTGIRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYIYCQIQSFFSIWFNMIVSICILFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQPKIRLIFMLLYSKILEILIFLINLTLFIFLKYINYKHIKMERQALRIGISISTQSDVDSDKLDIESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSESSWTRNDFINFIENAILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC 339          
BLAST of SMED30003912 vs. Planmine SMEST
Match: SMESG000001727.1 (SMESG000001727.1)

HSP 1 Score: 262.307 bits (669), Expect = 1.742e-84
Identity = 155/335 (46.27%), Postives = 224/335 (66.87%), Query Frame = 2
Query:  179 ISQESTITAIVFGLFSCIFSVFSFLILLRMKVTDCTTGIRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFN-QTHRNCTKSKYIYCQIQSFFSIWFNMIVSICILFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQP--KIRLIFMLLYSKXXXXXXXXXXXXXXXXXKYINYKHIKMERQALRIGISISTQSDVDSDKLDIESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSQSSWTRNDFINFIENA---ILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC 1165
            ++++ TI +IV G+ S +F + SF+IL++M+  +   G R+SSIFML NLVA+D LR     +G +RY  I       +R       ++C+IQSFFSIWFNMI+ +CILFIGIFA+YQ+ G     R I KK L++TSI+ W++PF +TLIPAI   LG+D T TTNGWCWI+SC +   +IR+++ML+YS+  + +IFLINLTLFI+LKYI Y   K         +SIST S + ++    ++  NDSKLLFLIN++IC CR+WG++RT +++  M+S  S      +  ++ +    + +Q IF+N+Q  FVF VL+LC NF K+Y+ RL C
Sbjct:   14 VNKDVTIISIVLGIISVVFGLISFVILVKMRFKERHIGFRVSSIFMLTNLVAADWLRGVSIFSGTVRYFIIFHTKYGPNRGDPCDADLFCRIQSFFSIWFNMIMLLCILFIGIFAVYQVAG-CQEDRYIGKKVLIVTSILCWTIPFFITLIPAITGALGEDQTVTTNGWCWITSCNRTTREIRVVYMLIYSRAFDAMIFLINLTLFIYLKYITYTSNKSTNNLKICNLSISTASYLITESFGKKNIRNDSKLLFLINLSICTCRIWGIVRTGLDISLMYSVDS----SIVKIVQGSSAIFMILQIIFDNIQTTFVFVVLLLCCNFVKTYLYRLIC 343          
BLAST of SMED30003912 vs. Planmine SMEST
Match: SMESG000001727.1 (SMESG000001727.1)

HSP 1 Score: 248.44 bits (633), Expect = 1.651e-79
Identity = 151/334 (45.21%), Postives = 216/334 (64.67%), Query Frame = 2
Query:  179 ISQESTITAIVFGLFSCIFSVFSFLILLRMKVTDCTTGIRMSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYIYCQIQSFFSIWFNMIVSICILFIGIFAIYQITGKTGYMRNIKKKTLVITSIVAWSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQP--KIRLIFMLLYSKXXXXXXXXXXXXXXXXXKYINYKHIKMERQALRIGISISTQSDVDSDKLDIESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSQSSWTRNDFINFIENA---ILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC 1165
            ++++ TI +IV G+ S +F + SF+IL++M+  +   G R+SSIFML NLVA+D LR     +G +R                       IQSFFSIWFNMI+ +CILFIGIFA+YQ+ G     R I KK L++TSI+ W++PF +TLIPAI   LG+D T TTNGWCWI+SC +   +IR+++ML+YS+  + +IFLINLTLFI+LKYI Y   K         +SIST S + ++    ++  NDSKLLFLIN++IC CR+WG++RT +++  M+S  S      +  ++ +    + +Q IF+N+Q  FVF VL+LC NF K+Y+ RL C
Sbjct:   14 VNKDVTIISIVLGIISVVFGLISFVILVKMRFKERHIGFRVSSIFMLTNLVAADWLRGVSIFSGTVR-----------------------IQSFFSIWFNMIMLLCILFIGIFAVYQVAG-CQEDRYIGKKVLIVTSILCWTIPFFITLIPAITGALGEDQTVTTNGWCWITSCNRTTREIRVVYMLIYSRAFDAMIFLINLTLFIYLKYITYTSNKSTNNLKICNLSISTASYLITESFGKKNIRNDSKLLFLINLSICTCRIWGIVRTGLDISLMYSVDS----SIVKIVQGSSAIFMILQIIFDNIQTTFVFVVLLLCCNFVKTYLYRLIC 319          
The following BLAST results are available for this feature:
BLAST of SMED30003912 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A1S3I5M67.456e-724.61G-protein coupled receptor 157 OS=Lingula unguis O... [more]
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BLAST of SMED30003912 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gpr1575.450e-624.38G protein-coupled receptor 157 [Source:NCBI gene;A... [more]
gpr1579.377e-624.38G protein-coupled receptor 157 [Source:NCBI gene;A... [more]
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BLAST of SMED30003912 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003912 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000001726.10.000e+099.71SMESG000001726.1[more]
SMESG000001727.11.742e-8446.27SMESG000001727.1[more]
SMESG000001727.11.651e-7945.21SMESG000001727.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003912 ID=SMED30003912|Name=SMED30003912|organism=Schmidtea mediterranea sexual|type=transcript|length=1218bp
TAAAAACTTTCAACTTGATATTGTGTTGGTAATTTATTTATAAATAAAAC
AAATATTACAGATTGATTGTGCGCATCATTCAACGAATGAACTTTGACAT
AAACCTCCTAAATTTTTACTAAAGCTCTAAATTACATTTCGAGAAAAGAT
GTCTGATGATTATTCGAGAGATTTTAAAATAAGCCAGGAATCGACAATTA
CAGCGATTGTCTTTGGATTATTTTCATGCATATTTTCTGTGTTTTCGTTT
CTAATCTTGCTGAGGATGAAAGTCACAGATTGTACAACTGGCATTCGGAT
GTCTTCAATTTTTATGCTCTTGAATTTGGTTGCCAGCGACGGCTTGAGGT
CTTTTTTTAATTTGACTGGAATGATAAGATATCTCACAATAATTGACTTC
AACCAAACTCATCGGAATTGTACAAAATCGAAATACATCTACTGCCAAAT
TCAGAGCTTTTTCAGTATTTGGTTTAACATGATTGTTTCAATATGTATTT
TGTTTATTGGAATTTTTGCCATTTATCAAATAACCGGAAAAACGGGTTAC
ATGAGAAATATCAAGAAGAAAACATTGGTCATTACTAGCATAGTAGCATG
GTCAGTTCCTTTTTTGCTAACTTTGATTCCAGCAATATGGCACAAACTCG
GACAGGATTTAACTAAAACGACAAACGGCTGGTGCTGGATTTCATCTTGC
GAGCAGCCAAAAATACGTTTAATTTTTATGCTTCTCTACAGCAAAATTTT
AGAAATTCTAATTTTTCTAATTAATTTGACCTTGTTTATTTTTCTCAAAT
ACATAAATTACAAACATATAAAAATGGAAAGACAGGCGTTGAGAATCGGT
ATTTCTATCAGCACTCAATCTGATGTCGACTCTGATAAACTGGACATAGA
AAGCTCCACAAATGATTCTAAACTCCTATTTTTAATCAACATCACCATTT
GTTGGTGTAGGGTCTGGGGTCTTATTAGAACTTTCATTGAAGTTGGTAAG
ATGTTCTCACAGAGCAGCTGGACTAGAAATGATTTCATAAATTTCATAGA
AAATGCCATATTGTTCATACAAGCCATTTTCGAAAATTTGCAAGGTGTAT
TTGTATTCTCGGTGCTAATTCTGTGTAGCAATTTTGCAAAGTCATACATA
CGTAGACTGTTCTGTTAATAAATAAAACAGTGGAAATTCTATATTAATAG
ATAATTCGTTAATTAAAA
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protein sequence of SMED30003912-orf-1

>SMED30003912-orf-1 ID=SMED30003912-orf-1|Name=SMED30003912-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=340bp
MSDDYSRDFKISQESTITAIVFGLFSCIFSVFSFLILLRMKVTDCTTGIR
MSSIFMLLNLVASDGLRSFFNLTGMIRYLTIIDFNQTHRNCTKSKYIYCQ
IQSFFSIWFNMIVSICILFIGIFAIYQITGKTGYMRNIKKKTLVITSIVA
WSVPFLLTLIPAIWHKLGQDLTKTTNGWCWISSCEQPKIRLIFMLLYSKI
LEILIFLINLTLFIFLKYINYKHIKMERQALRIGISISTQSDVDSDKLDI
ESSTNDSKLLFLINITICWCRVWGLIRTFIEVGKMFSQSSWTRNDFINFI
ENAILFIQAIFENLQGVFVFSVLILCSNFAKSYIRRLFC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR017981GPCR_2-like
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0004888transmembrane signaling receptor activity
Vocabulary: biological process
TermDefinition
GO:0007166cell surface receptor signaling pathway
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 6..327
e-value: 4.2E-17
score: 64.2
NoneNo IPR availablePANTHERPTHR23112G PROTEIN-COUPLED RECEPTOR 157-RELATEDcoord: 13..283
NoneNo IPR availablePANTHERPTHR23112:SF12G-PROTEIN COUPLED RECEPTOR 157coord: 13..283
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..27
score: 5.0
NoneNo IPR availableTMHMMTMhelixcoord: 57..79
NoneNo IPR availableTMHMMTMhelixcoord: 259..281
NoneNo IPR availableTMHMMTMhelixcoord: 143..165
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 104..126
NoneNo IPR availableTMHMMTMhelixcoord: 200..219
NoneNo IPR availableTMHMMTMhelixcoord: 304..326
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 29..269
score: 9.559
IPR017981GPCR, family 2-likePROSITEPS50261G_PROTEIN_RECEP_F2_4coord: 12..327
score: 12.255