SMED30003852

Overview
NameSMED30003852
Smed IDSMED30003852
Length (bp)1735
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003852 (SMED30003852) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003852 (SMED30003852) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003852 (SMED30003852) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003852 (SMED30003852) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003852

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30003852SMESG000068031.1 dd_Smed_v4_8885_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003852SMESG000068031.1 dd_Smed_v4_8885_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30003852SMESG000068031.1 dd_Smed_v4_8885_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003852SMESG000068031.1 GCR159smed_ncbi_20200123PMID:27163480
Saberi et al., 2016		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003852 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.022100:7484..9218 -8675
Homology
[Close]
BLAST of SMED30003852 vs. Ensembl Xenopus
Match: dlec1 (DLEC1, cilia and flagella associated protein [Source:Xenbase;Acc:XB-GENE-990958])

HSP 1 Score: 50.447 bits (119), Expect = 4.881e-6
Identity = 33/104 (31.73%), Postives = 49/104 (47.12%), Query Frame = -3 
Query:  381 YLISLHSNLNRLIDQHSTI-----SSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDK--NFQIPIGLYRALTCLAWSNSFLEPLIYLLL 671
            ++ S+  N  +L+  H +       S+IS++  +K A           TLS V G+ + CW P F L + D   NF +P  LY     L + NS L P+IY L 
Sbjct:  210 HIFSVARNQAKLVHNHHSFQPDKSKSKISVRAERKAAK----------TLSIVMGVFLFCWLPFFILTVIDPYINFSVPEDLYNVFLWLGYFNSALNPIIYALF 303
[Close]
BLAST of SMED30003852 vs. TrEMBL
Match: A0A193KUP6 (GCR159 OS=Schmidtea mediterranea OX=79327 GN=gcr159 PE=2 SV=1)

HSP 1 Score: 724.546 bits (1869), Expect = 0.000e+0
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = -3 
Query:  141 MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYXXXXXXXXXXXXXLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCCLKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCAQPFERSQNPDYRTWKNNNFLEC 1256
            MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYVVFVVWIVIVTLVLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCCLKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCAQPFERSQNPDYRTWKNNNFLEC
Sbjct:    1 MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYVVFVVWIVIVTLVLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCCLKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCAQPFERSQNPDYRTWKNNNFLEC 372
[Close]
BLAST of SMED30003852 vs. TrEMBL
Match: C9K4W2 (4656HH OS=Dugesia japonica OX=6161 GN=4656HH PE=2 SV=1)

HSP 1 Score: 645.195 bits (1663), Expect = 0.000e+0
Identity = 342/372 (91.94%), Postives = 354/372 (95.16%), Query Frame = -3 
Query:  141 MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYXXXXXXXXXXXXXLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCCLKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCAQPFERSQNPDYRTWKNNNFLEC 1256
            MTAVSDEYQWHTNY+LEVAILYSIAALGIILNFVQT CILIEKEIRNLLSAFLIHGAIINAVLASYC+VFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLN+YE DLN N +KYNNICCV+FGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYVVFVVWI+IVTLVLAACTYYLISLHSNLNRLI QHSTISSR SLQKNQK  DEL+LTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNF IPIGLYRALTCLAWSNSFLEPLIYLLLDRK+IRCCLKLVSNS+L      NRSSRLQRIGDRLKSAP+EN+ +IELRQISNYDCAQ  ERSQNPDYRTWKNNNFLEC
Sbjct:    1 MTAVSDEYQWHTNYNLEVAILYSIAALGIILNFVQTLCILIEKEIRNLLSAFLIHGAIINAVLASYCVVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNFYECDLNNNNSKYNNICCVIFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYVVFVVWIIIVTLVLAACTYYLISLHSNLNRLIYQHSTISSRTSLQKNQKVTDELILTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFLIPIGLYRALTCLAWSNSFLEPLIYLLLDRKMIRCCLKLVSNSRLVN-NEINRSSRLQRIGDRLKSAPLENDFDIELRQISNYDCAQHLERSQNPDYRTWKNNNFLEC 371
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BLAST of SMED30003852 vs. TrEMBL
Match: T1FMP0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Helobdella robusta OX=6412 GN=20210089 PE=4 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.080e-57
Identity = 132/342 (38.60%), Postives = 193/342 (56.43%), Query Frame = -3 
Query:  297 FNAQQR*MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVY---KLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYXXXXXXXXXXXXXLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADE-------LVL--TKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRK---VIRCCLKLVSNSQLTELTGNNRSSR 1277
            FN Q      V        NY+ E   L  IA++G+I N V   C+LI + +R   SAF+IHG I++AV   YC+ FA+SL        C  LG SY+V++TA+ FN++A++  E Y   + NY   + + ++ +  N CCV FG+ +VY +SLI+HLGPT+IGG+FN++   G C+F++G  K+YV   +WIVI TL +    YYLI  H ++    +       R S+  +Q   DE       LV   T +R   L  +T + +L WYPL+ L +SD  FQ P  +Y+ LT +AWSN+ + P+I++L D+    V RCC  +V         GNNRSSR
Sbjct:   30 FNKQHDGSEQVETRAVSQENYN-EAVFLLCIASIGLISNIVVVICVLINRNLRKSASAFVIHGCILDAVRCLYCVPFAVSLLWDMAPGFCAALGGSYVVVVTASGFNIVAMVCCEAYVFSERNYGVENASNSSGENGNFCCVAFGVLMVYATSLIIHLGPTIIGGDFNYNDLIGNCIFVYGTMKSYVAQTMWIVITTLSMIGTVYYLIYFHKHVQTHSNHRLASLVRASIVVSQGNVDETDHLTAQLVFQQTLARCRVLIIITVVFILSWYPLYILTLSDPKFQQPSKIYKLLTFIAWSNAAINPIIFVLFDQGIDVVRRCCDCIVP--------GNNRSSR 362
[Close]
BLAST of SMED30003852 vs. TrEMBL
Match: R7TXV4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_208991 PE=4 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 1.582e-49
Identity = 114/329 (34.65%), Postives = 182/329 (55.32%), Query Frame = -3 
Query:  357 LNRIFNAQQR*MTAVSDEYQWH------TNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYXXXXXXXXXXXXXLAACTYYLISL--HSNLNRLIDQHSTISSRISLQKNQKTADELVL----TKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDR------KVIRCC 1289
            +N    +QQ  +  ++D +         T+   E   L+++A  GI+LN +   CI   + +R + SAF+ HG +++ +   +CI FA SL +    + C  LG SY++++TA+ FN++A I  E Y  + +    N   +   ++CCV+FG+++VYV S+I+HLGPT+IGG F F+   G C+F +G  ++YVV  +WIVI+TL +    YYL+    H   N      S + + I++ +    + ++      + SR   L  +T L+V+CWYPLF L + D  F  P  +YR LT +AWSN  L PL+ LL DR      + I CC
Sbjct:    1 MNATAASQQPQLQQIADRHDDQVAASSTTDSVHEALFLFAVAGAGIVLNAIVLACIFFCRHLRRMTSAFVFHGTVLDVIKCIFCIPFATSLLNDLEPDFCGVLGGSYVIMVTASGFNIVASICCEAYTFSEH----NVGGDGRGSLCCVMFGITMVYVGSIIIHLGPTIIGGVFKFNEAIGNCIFKYGTVQSYVVHAMWIVIMTLSMVGAIYYLVFFYRHVQANSTHRLASLVRASIAVSRGNTDSQDIRKIIRESLSRTRVLILITALYVICWYPLFILTLLDPGFLQPPRVYRLLTFVAWSNGALNPLVMLLFDRNLSVLKRRIVCC 325
[Close]
BLAST of SMED30003852 vs. TrEMBL
Match: K1Q4M3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Crassostrea gigas OX=29159 GN=CGI_10019066 PE=4 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 4.592e-43
Identity = 99/290 (34.14%), Postives = 165/290 (56.90%), Query Frame = -3 
Query:  369 YSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYXXXXXXXXXXXXXLAACTYYLISL-------HSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKV 1217
            Y  E   LY +A LG++LN +    I I + +R L SAFL+H   ++++ A+YCI  A +L  +   + C + G++Y++I+TA+IFN++A++ +E Y       ++N   N + ++CCV FG+ +VY++S ILHLGPTLIGG F+F  Q G C F+ G+   YV  V+WI+I+TL +    +++  L       H N   ++ + S   +   L         +  +  RA      T   VLCWYPLF L++ D  F+    +Y+A + +AW+   ++P++Y+  DR +
Sbjct:   46 YHQEAYYLYFVAVLGLLLNILVAIFIFIRRTMRKLTSAFLVHACFLDSLKAAYCIPIANNLITQIKPSDCDFFGATYVLIITASIFNMVAMVCTEAYTF----GEVNIGGNSHGSLCCVFFGIILVYIASTILHLGPTLIGGYFDFHPQIGSCSFVLGKQTGYVAHVMWIIIITLAVLFVIHFICKLYKEIQMNHPNRVSMLVRSSITITDNPLNSTCNIRHMIKDSSQRAKMFVLSTIAFVLCWYPLFLLILVDSEFKQRPKMYQAFSFIAWTQGTIQPILYICFDRHI 331
[Close]
BLAST of SMED30003852 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000271.1 (pep scaffold:Pmarinus_7.0:GL476504:17018:19617:1 gene:ENSPMAG00000000243.1 transcript:ENSPMAT00000000271.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.9802 bits (110), Expect = 6.341e-6
Identity = 66/277 (23.83%), Postives = 117/277 (42.24%), Query Frame = -3 
Query:  372 CILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKS-----FQNL-CKYLGSSYIVIMTANIFNLIAIIASE---VYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGN----FNFDSQTGFCLFIFGQTKNYXXXXXXXXXXXXXLAACT-YYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLIS-----DKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRK 1145
            C L+E+ +R +   F++  A  + ++A  C+V   S+  +      F  L CK   S  ++  TA+I  L  I       V   N+Y   ++          C+   +SI +V SL++   P   G N      FD++   CLFI      +V+    +      LA CT Y+ +   S L        T+   I    N +  +   L      T+S +    V+CW P F   ++      K+ ++ + +++ +T L + NS + P++Y  L+R 
Sbjct:   47 CPLVERRLRTVTYMFIMSLATADLLVA--CLVMPFSIIYEVTGVWIFGELFCKVWISFDVMFCTASIVTLCFISLDRYCSVTTPNHYPTRMSGKR-------CIAMTVSI-WVYSLLISFLPVTQGWNEIPGVRFDARRE-CLFI--TNWAFVLVASTLAFYLPFLAMCTMYFFVYRASRLKAARVASQTLD--IHYHPNSQRPNNPQLENKATRTMSIIISAFVMCWLPYFVFNVAMNAYGSKHDRVVVTMFKIMTWLGYCNSAINPVLYAFLNRD 308
[Close]
BLAST of SMED30003852 vs. Planmine SMEST
Match: SMESG000068031.1 (SMESG000068031.1)

HSP 1 Score: 724.546 bits (1869), Expect = 0.000e+0
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = -3 
Query:  141 MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYXXXXXXXXXXXXXLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCCLKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCAQPFERSQNPDYRTWKNNNFLEC 1256
            MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYVVFVVWIVIVTLVLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCCLKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCAQPFERSQNPDYRTWKNNNFLEC
Sbjct:    1 MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLSAFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFNLIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGPTLIGGNFNFDSQTGFCLFIFGQTKNYVVFVVWIVIVTLVLAACTYYLISLHSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLCWYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCCLKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCAQPFERSQNPDYRTWKNNNFLEC 372
The following BLAST results are available for this feature:
BLAST of SMED30003852 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dlec14.881e-631.73DLEC1, cilia and flagella associated protein [Sour... [more]
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BLAST of SMED30003852 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUP60.000e+0100.00GCR159 OS=Schmidtea mediterranea OX=79327 GN=gcr15... [more]
C9K4W20.000e+091.944656HH OS=Dugesia japonica OX=6161 GN=4656HH PE=2 ... [more]
T1FMP01.080e-5738.60G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
R7TXV41.582e-4934.65G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
K1Q4M34.592e-4334.14G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of SMED30003852 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000000271.16.341e-623.83pep scaffold:Pmarinus_7.0:GL476504:17018:19617:1 g... [more]
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BLAST of SMED30003852 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003852 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000068031.10.000e+0100.00SMESG000068031.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003852 ID=SMED30003852|Name=SMED30003852|organism=Schmidtea mediterranea sexual|type=transcript|length=1735bp
TGTTGTTTACTGTTGATTAGTCATTAACAACGTTATAAAATATATTTGGC
TGAAATACATTTGTTATGTATTTTGACAATTTAAAAGTATAATCTCAAAA
CAATGGAAAATTATAATTGACAATTAATATTAACAATTCAACATTCTAAA
AAATTATTATTTTTCCAAGTTCTATAATCGGGATTTTGTGAGCGCTCGAA
AGGCTGGGCACAATCGTAGTTGGAAATTTGTCGTAATTCGATTTCGATTT
CATTTTCAATCGGTGCTGACTTCAATCTGTCACCGATTCTCTGGAGTCTG
GAAGATCGATTGTTGCCAGTCAATTCGGTCAGTTGTGAATTGGACACCAA
CTTCAAACAGCAGCGAATGACTTTCCGATCAAGTAGTAAGTAAATCAATG
GCTCAAGAAATGAATTCGACCAAGCCAGACATGTCAATGCCCGATAAAGA
CCGATGGGTATTTGGAAATTCTTATCGGAAATCAGCAAACCAAACAACGG
ATACCAACATAAAACATGAAGCCCAGTGACGCAACTCAGTGTCCATGCTC
GACTTTTTGTCAATACCAATTCATCTGCGGTTTTTTGATTTTTCTGCAGC
GATATCCGTGAGCTGATTGTCGAATGCTGGTCAATCAACCGATTCAAGTT
CGAATGCAGTGATATGAGATAGTACGTGCATGCTGCTAGAACCAATGTCA
CAATGACAATCCACACGACAAAAACCACATAGTTTTTCGTCTGGCCGAAA
ATAAATAAACAAAACCCAGTTTGACTGTCGAAGTTGAAATTACCACCAAT
AAGTGTCGGGCCTAAATGGAGAATTAAGCTCGAAACGTACACGATCGATA
AGCCGAACACGACGCAGCATATATTATTATATTTATTAGTATTTGCATTC
AAATCGTTTTCATAGTAATTGAGCTTATAGACCTCACTGGCGATTATTGC
AATCAGATTGAATATATTGGCCGTCATTATTACAATATACGAACTGCCTA
AATATTTACATAAATTTTGGAACGACTTATCGTGCAAACTCAATGCAAAA
ACTATACAATATGAAGCTAATACAGCGTTGATAATAGCACCGTGAATTAG
AAAAGCTGATAGGAGGTTTCGGATTTCCTTTTCAATCAAAATACAAAACG
TTTGGACGAAATTTAAAATAATGCCTAATGCTGCAATTGAGTAAAGAATA
GCGACTTCCAAACTGTAGTTAGTATGCCACTGATATTCATCACTGACAGC
AGTCATTTAACGTTGTTGAGCATTGAAAATCCGATTCAATATTTTTTTTA
AAGTCTGAAATTTCTGTTCCGAAATTGAGATTTTAATAATTCCACAAGAT
TTTTTAATTTATCGATGATTCATAATGGCTAAATCTGTATCCAATGACAA
AAGATTAAATCAAAGAAATTTCATAGTAAAACAAATAATGCAACCAATTG
TAGACGAGAAATTCAGATCAGCTATTATGTCGTTGGCAAATAATCTGAAT
GTCATGACCAGCTGTATTGACTACGTTCACACACACCAACACAACAACGA
ACTGAAATTGTCCCACATACAGACAATTCATTCAGACAACTGTGTGTGTG
TTTCAACCTTGTCAATTCAACACACTTTCTTTTCGCAAACACACACACAC
ACAATCTTTTGGGATAGAGAATTTGTTTGTTTGATTTCAAATTACCATTT
TCTTGAGTTGATTAGTGTATGTATTAAAACAACTG
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protein sequence of SMED30003852-orf-1

>SMED30003852-orf-1 ID=SMED30003852-orf-1|Name=SMED30003852-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=120bp
MAKSVSNDKRLNQRNFIVKQIMQPIVDEKFRSAIMSLANNLNVMTSCIDY
VHTHQHNNELKLSHIQTIHSDNCVCVSTLSIQHTFFSQTHTHTIFWDREF
VCLISNYHFLELISVCIKTT
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protein sequence of SMED30003852-orf-2

>SMED30003852-orf-2 ID=SMED30003852-orf-2|Name=SMED30003852-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=373bp
MTAVSDEYQWHTNYSLEVAILYSIAALGIILNFVQTFCILIEKEIRNLLS
AFLIHGAIINAVLASYCIVFALSLHDKSFQNLCKYLGSSYIVIMTANIFN
LIAIIASEVYKLNYYENDLNANTNKYNNICCVVFGLSIVYVSSLILHLGP
TLIGGNFNFDSQTGFCLFIFGQTKNYVVFVVWIVIVTLVLAACTYYLISL
HSNLNRLIDQHSTISSRISLQKNQKTADELVLTKSRAWTLSCVTGLHVLC
WYPLFGLLISDKNFQIPIGLYRALTCLAWSNSFLEPLIYLLLDRKVIRCC
LKLVSNSQLTELTGNNRSSRLQRIGDRLKSAPIENEIEIELRQISNYDCA
QPFERSQNPDYRTWKNNNFLEC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000044cephalic ganglia
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 271..297
score: 24.71
coord: 90..112
score: 17.17
coord: 16..40
score: 15.67
coord: 236..260
score: 23.74
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 32..289
e-value: 5.5E-12
score: 45.6
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 2..335
e-value: 2.5E-20
score: 74.2
NoneNo IPR availablePANTHERPTHR24240OPSINcoord: 19..322
NoneNo IPR availablePANTHERPTHR24240:SF134coord: 19..322
NoneNo IPR availableCDDcd006377tm_classA_rhodopsin-likecoord: 17..292
e-value: 3.35096E-10
score: 58.0578
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 7..308
NoneNo IPR availableTMHMMTMhelixcoord: 85..107
NoneNo IPR availableTMHMMTMhelixcoord: 270..292
NoneNo IPR availableTMHMMTMhelixcoord: 238..260
NoneNo IPR availableTMHMMTMhelixcoord: 178..200
NoneNo IPR availableTMHMMTMhelixcoord: 48..70
NoneNo IPR availableTMHMMTMhelixcoord: 19..41
NoneNo IPR availableTMHMMTMhelixcoord: 128..150
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 31..289
score: 12.247