SMED30003838

Overview
NameSMED30003838
Smed IDSMED30003838
Length (bp)2878
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003838 (SMED30003838) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003838 (SMED30003838) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003838 (SMED30003838) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003838 (SMED30003838) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003838

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30003838SMESG000026730.1 dd_Smed_v4_13017_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003838SMESG000026730.1 dd_Smed_v4_13017_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30003838SMESG000026730.1 dd_Smed_v4_13017_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003838 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000564:131034..133974 +14125
Homology
[Close]
BLAST of SMED30003838 vs. Ensembl Human
Match: PDE11A (phosphodiesterase 11A [Source:HGNC Symbol;Acc:HGNC:8773])

HSP 1 Score: 53.1434 bits (126), Expect = 3.128e-6
Identity = 34/97 (35.05%), Postives = 56/97 (57.73%), Query Frame = 3 
Query:  288 IKVDSTYDE-------------RRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKC-LRETNWTEFFVEIVSGVLSDLDYQSVINKI 536
            I V+ TYDE             RR +L  +R+ +SLP TT++ L++L+ S++ LP++       KC L++ N  +FF+E+V  + +DLD  S+  KI
Sbjct:  131 IHVNRTYDEQVTSRAQEPLSSVRRRAL--LRKASSLPPTTAHILSALLESRVNLPRYPPTAIDYKCHLKKHNERQFFLELVKDISNDLDLTSLSYKI 225
[Close]
BLAST of SMED30003838 vs. Ensembl Mouse
Match: Pde11a (phosphodiesterase 11A [Source:MGI Symbol;Acc:MGI:3036251])

HSP 1 Score: 52.373 bits (124), Expect = 2.843e-6
Identity = 34/97 (35.05%), Postives = 56/97 (57.73%), Query Frame = 3 
Query:  288 IKVDSTYDE-------------RRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKC-LRETNWTEFFVEIVSGVLSDLDYQSVINKI 536
            I V+ TYDE             RR +L  +R+ +SLP TT++ L++L+ S++ LP++       KC L++ N  +FF+E+V  + +DLD  S+  KI
Sbjct:  131 IHVNRTYDEQVTSRAQEPLSSVRRRAL--LRKASSLPPTTAHILSALLESRVNLPQYPPTAIDYKCHLKKHNERQFFLELVKDISNDLDLTSLSYKI 225
[Close]
BLAST of SMED30003838 vs. TrEMBL
Match: A0A2T7PQX7 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_02805 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.634e-10
Identity = 53/166 (31.93%), Postives = 88/166 (53.01%), Query Frame = 3 
Query:   60 VEERFFQLATPDLIRKWIKKFGIINLGIPNDI--NNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFL-QDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNM 548
            +E+ F + A   L+ +W+ + G+  L   + +   +   + +         T+F E G   + +      +     EP   DS+ + RR SL+ MRQF SLP T+ N L+ LI SK+ LP++  +D +H + LR TN   FF+EIV  + +DLD +S+ +K+  NM
Sbjct:   19 MEDYFLRKADIGLVNRWLTQHGLNALPEMSPVIGKHMSLSADSSAPSSPVDTRFGESG-FFESRHHRSNSKKFRTYEPTP-DSSAESRRSSLKEMRQFRSLPPTSVNMLSLLIQSKVRLPRYPSKDIDHKRELRYTNERAFFLEIVKDISNDLDLKSLSSKMVANM 182
[Close]
BLAST of SMED30003838 vs. TrEMBL
Match: A0A0B6Y003 (Uncharacterized protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF7838 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 3.562e-10
Identity = 37/77 (48.05%), Postives = 52/77 (67.53%), Query Frame = 3 
Query:  270 ASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFL-QDENHLKCLRETNWTEFFVEIVSGV 497
            +S+L     +ST + RR SL+ MRQF SLP T+ N L+ LI SKI LP++  +D +H + L+ETN  EFF+EIV  +
Sbjct:    1 SSDLPTTGGESTLESRRNSLKEMRQFRSLPPTSVNLLSMLIQSKIRLPRYPSKDIDHKRELKETNEREFFLEIVKDI 77
[Close]
BLAST of SMED30003838 vs. TrEMBL
Match: A0A2T7PQZ9 (PDEase domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_02818 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 9.910e-10
Identity = 42/90 (46.67%), Postives = 60/90 (66.67%), Query Frame = 3 
Query:  282 EPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFL-QDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNM 548
            EP   DS+ + RR SL+ MRQF SLP T+ N L+ LI SK+ LP++  +D +H + LR TN   FF+EIV  + +DLD +S+ +K+  NM
Sbjct:  108 EPTP-DSSAESRRSSLKEMRQFRSLPPTSVNMLSLLIQSKVRLPRYPSKDIDHKRELRYTNERAFFLEIVKDISNDLDLKSLSSKMVANM 196
[Close]
BLAST of SMED30003838 vs. TrEMBL
Match: A0A3S0ZNH7 (GAF domain-containing protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_020684 PE=4 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 1.834e-9
Identity = 40/88 (45.45%), Postives = 60/88 (68.18%), Query Frame = 3 
Query:  297 DSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKF-LQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 557
            +ST + RR SL+ MRQF SLP T+ N L+ LI SKI LP++  +D +H + L++TN  EFF+EIV  + ++LD +S+  KI  N+  +
Sbjct:  128 ESTLESRRNSLKEMRQFRSLPPTSVNLLSMLIQSKIRLPRYPSKDIDHKRDLKDTNEREFFLEIVKDISNELDLKSLTAKIVGNVSCM 215
[Close]
BLAST of SMED30003838 vs. TrEMBL
Match: V4B518 (Phosphodiesterase OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_236563 PE=3 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 4.919e-9
Identity = 41/84 (48.81%), Postives = 55/84 (65.48%), Query Frame = 3 
Query:  309 DERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKF-LQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 557
            D RR SL+ MRQF SLP  + N L+ LI S+I LP++  +D +  K LR TN  EFF+EIV  + +DLD +S+  KI  NM +L
Sbjct:  107 DARRSSLKEMRQFRSLPPNSVNMLSMLIQSRIRLPRYPSKDIDSKKELRYTNEKEFFLEIVKDISNDLDLRSLSTKIVANMCVL 190
[Close]
BLAST of SMED30003838 vs. Ensembl Cavefish
Match: pde11al (dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A-like [Source:NCBI gene;Acc:103024879])

HSP 1 Score: 53.1434 bits (126), Expect = 1.325e-6
Identity = 34/117 (29.06%), Postives = 64/117 (54.70%), Query Frame = 3 
Query:  249 KIQMIRGASNLEPIKVDSTYDE-------------RRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKC-LRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 557
             +++ R  +  +      TYDE             RR +L  +R+ +SLP TT++ L++L+ S++++P++       KC L+E N  EFF+E+V  + ++LD  ++  KI  N+ I+
Sbjct:   73 HVELRRSFARSKASTAHRTYDEHVSLSAHESQSSVRRRAL--LRKASSLPPTTAHILSALLESRVSVPRYPSSALDYKCRLKENNEREFFLELVKDISNELDMTNLSYKILINVCIM 187
[Close]
BLAST of SMED30003838 vs. Ensembl Cavefish
Match: pde11al (dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A-like [Source:NCBI gene;Acc:103024879])

HSP 1 Score: 53.1434 bits (126), Expect = 1.565e-6
Identity = 34/117 (29.06%), Postives = 64/117 (54.70%), Query Frame = 3 
Query:  249 KIQMIRGASNLEPIKVDSTYDE-------------RRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKC-LRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 557
             +++ R  +  +      TYDE             RR +L  +R+ +SLP TT++ L++L+ S++++P++       KC L+E N  EFF+E+V  + ++LD  ++  KI  N+ I+
Sbjct:   73 HVELRRSFARSKASTAHRTYDEHVSLSAHESQSSVRRRAL--LRKASSLPPTTAHILSALLESRVSVPRYPSSALDYKCRLKENNEREFFLELVKDISNELDMTNLSYKILINVCIM 187
[Close]
BLAST of SMED30003838 vs. Ensembl Medaka
Match: ENSORLT00000009688.2 (pep primary_assembly:ASM223467v1:16:16300402:16302109:-1 gene:ENSORLG00000007734.2 transcript:ENSORLT00000009688.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 50.0618 bits (118), Expect = 4.179e-6
Identity = 27/75 (36.00%), Postives = 49/75 (65.33%), Query Frame = 3 
Query:  336 MRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKC-LRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 557
            +R+ +SLP TT++ L++L+ S++ +P++       K  L+ETN  EFF+E+V  + ++LD  ++  KI  N+ IL
Sbjct:   19 LRKASSLPPTTAHILSALLESRVNVPQYASSAIDYKYRLKETNEREFFLELVKDISNELDLTNLSYKILINVCIL 93
[Close]
BLAST of SMED30003838 vs. Planmine SMEST
Match: SMESG000026730.1 (SMESG000026730.1)

HSP 1 Score: 350.903 bits (899), Expect = 7.573e-164
Identity = 170/171 (99.42%), Postives = 171/171 (100.00%), Query Frame = 3 
Query:   45 MDSKEVEERFFQLATPDLIRKWIKKFGIINLGIPNDINNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 557
            MDSKE+EERFFQLATPDLIRKWIKKFGIINLGIPNDINNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL
Sbjct:    1 MDSKEIEERFFQLATPDLIRKWIKKFGIINLGIPNDINNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 171

HSP 2 Score: 247.669 bits (631), Expect = 7.573e-164
Identity = 119/123 (96.75%), Postives = 120/123 (97.56%), Query Frame = 2 
Query:  548 GNPTYSDHCVMHINDKLVGSSNFSPEIVNYFLQISRKCRKENKPQKYQNDEVPECLHSSGIGELLCISLHLKSHDSVRPPKLSAALILINPTSSKNILQVSFSDFIHFNTCIAPVYFANINFC 916
            G  TYSDHCVMHINDKLVGSSNFSPEIVNYFLQISRKCRKENKPQKYQNDEVPECLHSSGIGELLCISLHLKSHDSVRPPKLSAALILINPTSSKN+LQVSFSDFIHFNTCIA VYFANINFC
Sbjct:  169 GILTYSDHCVMHINDKLVGSSNFSPEIVNYFLQISRKCRKENKPQKYQNDEVPECLHSSGIGELLCISLHLKSHDSVRPPKLSAALILINPTSSKNVLQVSFSDFIHFNTCIASVYFANINFC 291
[Close]
BLAST of SMED30003838 vs. Planmine SMEST
Match: SMESG000026730.1 (SMESG000026730.1)

HSP 1 Score: 354.755 bits (909), Expect = 1.864e-151
Identity = 170/171 (99.42%), Postives = 171/171 (100.00%), Query Frame = 3 
Query:   45 MDSKEVEERFFQLATPDLIRKWIKKFGIINLGIPNDINNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 557
            MDSKE+EERFFQLATPDLIRKWIKKFGIINLGIPNDINNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL
Sbjct:    1 MDSKEIEERFFQLATPDLIRKWIKKFGIINLGIPNDINNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDSTYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKCLRETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGIL 171

HSP 2 Score: 202.986 bits (515), Expect = 1.864e-151
Identity = 101/124 (81.45%), Postives = 105/124 (84.68%), Query Frame = 2 
Query:  548 GNPTYSDHCVMHINDKLVGSSNFSPEIVNYFLQISRKCRKENKPQKYQNDEVPECLHSSGIGELLCISLHLKSHDSVRPPKLSAALILINPTSSKNILQVSFSDFIHFNT----CIAPVYFANI 907
            G  TYSDHCVMHINDKLVGSSNFSPEIVNYFLQISRKCRKENKPQKYQNDEVPECLHSSGIGELLCISLHLKSHDSVRPPKLSAALILINPTSSKN+LQ       H N     C+  +Y A +
Sbjct:  169 GILTYSDHCVMHINDKLVGSSNFSPEIVNYFLQISRKCRKENKPQKYQNDEVPECLHSSGIGELLCISLHLKSHDSVRPPKLSAALILINPTSSKNVLQ-------HLNQFTQCCVKGLYNATL 285
[Close]
BLAST of SMED30003838 vs. Planmine SMEST
Match: SMESG000039356.1 (SMESG000039356.1)

HSP 1 Score: 50.447 bits (119), Expect = 2.421e-8
Identity = 22/40 (55.00%), Postives = 29/40 (72.50%), Query Frame = 3 
Query: 2535 PFRICFDLICRFTCNGRNKTFIEETSRHFWTQV*EHARIE 2654
            P  +C DL+ +FTCNG N T+I ETSRH  T+V EH R++
Sbjct:  878 PVGLCSDLVYQFTCNGCNATYIGETSRHLCTRVLEHCRLK 917

HSP 2 Score: 28.1054 bits (61), Expect = 2.421e-8
Identity = 15/40 (37.50%), Postives = 26/40 (65.00%), Query Frame = 2 
Query: 2405 VLRFAESFRNFEKAILNNIGSINVKIISKYFKVCDVFSNK 2524
            VL ++ESF N+++ +   IG++  KI+S   K  ++F NK
Sbjct:  835 VLPYSESFTNYKRNMGKLIGNLEYKIVSNSCKGRNMFINK 874
[Close]
BLAST of SMED30003838 vs. Planmine SMEST
Match: SMESG000040932.1 (SMESG000040932.1)

HSP 1 Score: 52.7582 bits (125), Expect = 4.590e-8
Identity = 32/95 (33.68%), Postives = 52/95 (54.74%), Query Frame = 3 
Query: 2535 PFRICFDLICRFTCNGRNKTFIEETSRHFWTQV*EHARIE*TQ*YYCA**NM*VENLYFQL*VLSNGFRNFFEYIAYEA*IIKSF*PKVNM*NSI 2819
            P  +C DL+ +FTCNG N T+I ETSRH  T+V EH R++              +       +L   F +++E +  EA +I+S  PK+N+ +++
Sbjct:    9 PVGLCSDLVYQFTCNGCNATYIGETSRHLCTRVLEHCRLKGLTNISEHNRGCKSDIGMSDFRILLRSFNSYWERVICEALLIRSLDPKINVQSAV 103
[Close]
BLAST of SMED30003838 vs. Planmine SMEST
Match: SMESG000027381.1 (SMESG000027381.1)

HSP 1 Score: 52.373 bits (124), Expect = 4.178e-7
Identity = 32/95 (33.68%), Postives = 52/95 (54.74%), Query Frame = 3 
Query: 2535 PFRICFDLICRFTCNGRNKTFIEETSRHFWTQV*EHARIE*TQ*YYCA**NM*VENLYFQL*VLSNGFRNFFEYIAYEA*IIKSF*PKVNM*NSI 2819
            P  +C DL+ +FTCNG N T+I ETSRH  T+V EH R++              +       +L   F +++E +  EA +I+S  PK+N+ +++
Sbjct:  113 PVGLCSDLVYQFTCNGCNATYIGETSRHLCTRVLEHCRLKGLTNISEHNRGCKSDIGMSDFRILLRSFNSYWERVICEALLIRSLDPKINVQSAV 207
The following BLAST results are available for this feature:
BLAST of SMED30003838 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PDE11A3.128e-635.05phosphodiesterase 11A [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of SMED30003838 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Pde11a2.843e-635.05phosphodiesterase 11A [Source:MGI Symbol;Acc:MGI:3... [more]
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BLAST of SMED30003838 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7PQX71.634e-1031.93Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A0B6Y0033.562e-1048.05Uncharacterized protein (Fragment) OS=Arion vulgar... [more]
A0A2T7PQZ99.910e-1046.67PDEase domain-containing protein OS=Pomacea canali... [more]
A0A3S0ZNH71.834e-945.45GAF domain-containing protein (Fragment) OS=Elysia... [more]
V4B5184.919e-948.81Phosphodiesterase OS=Lottia gigantea OX=225164 GN=... [more]
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BLAST of SMED30003838 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pde11al1.325e-629.06dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 1... [more]
pde11al1.565e-629.06dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 1... [more]
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BLAST of SMED30003838 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003838 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000009688.24.179e-636.00pep primary_assembly:ASM223467v1:16:16300402:16302... [more]
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BLAST of SMED30003838 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000026730.17.573e-16499.42SMESG000026730.1[more]
SMESG000026730.11.864e-15199.42SMESG000026730.1[more]
SMESG000039356.12.421e-855.00SMESG000039356.1[more]
SMESG000040932.14.590e-833.68SMESG000040932.1[more]
SMESG000027381.14.178e-733.68SMESG000027381.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003838 ID=SMED30003838|Name=SMED30003838|organism=Schmidtea mediterranea sexual|type=transcript|length=2878bp
TCACAATTTGCATTATCAAAGAATTCCAGTTTTTAATATTTTTAATGGAT
TCCAAAGAGGTAGAAGAAAGATTTTTCCAGCTTGCAACTCCTGATCTGAT
CAGAAAGTGGATTAAAAAGTTTGGCATAATAAATTTAGGCATTCCGAATG
ATATTAACAATCAATTTGAAAACTGCAACAAATTTTGCACTGGAAAAATC
AAAAAAACCCAATTTGTTGAACAAGGAGAAATTGTCGATTGTAAAAGGAA
AATTCAAATGATTCGAGGCGCATCTAACTTGGAACCGATCAAAGTCGATT
CGACGTATGATGAACGTCGAGGAAGTCTTCAGACCATGAGACAATTTAAC
TCATTGCCATCGACGACGTCGAATAATCTTGCATCGTTAATCAACTCGAA
AATCACATTGCCAAAGTTTCTCCAAGACGAAAACCATTTGAAATGTTTAC
GAGAAACGAATTGGACGGAGTTCTTTGTTGAAATCGTTTCTGGCGTTTTG
AGTGATTTGGATTACCAATCAGTTATTAACAAAATCCGCACAAATATGGG
AATCCTACGTATTCGGATCACTGTGTCATGCACATAAATGACAAATTAGT
TGGCAGTTCGAACTTTTCTCCGGAAATTGTCAATTATTTTTTACAAATCT
CCCGAAAATGCAGAAAAGAAAACAAGCCGCAAAAGTATCAAAATGACGAA
GTACCTGAATGTCTTCACAGTTCCGGAATTGGGGAACTGCTCTGCATCTC
GCTCCATTTGAAATCTCACGACTCAGTTAGGCCTCCCAAATTATCAGCAG
CCTTAATATTGATAAATCCTACCAGTAGCAAAAATATTCTTCAGGTCAGT
TTTTCAGATTTTATACATTTCAACACTTGTATTGCGCCCGTATACTTTGC
AAACATAAACTTCTGCTGACGCCTATTAAAACTTTATTCTAAATAGCGGC
ACTTATTCCCAAATAAAGAAAATTATAAAGGGAAAATAAAAATAGGCGGA
AAATTAAAATTGAATATTTTCATTCAATTTTCAACAATCGAAAACACTGA
TCTATAGTCAAAAACGAATTGTAAATAAATGAAATTCTATATGAAAACCA
TCCAGAGGTGAAGAAGAGAACGATAAATAATGTTATTGAAAAGTTTTAAG
GTTTTAAATTACAAAAGGTGATTAAAACATGAATTTGAAATTAGAATATT
TCTATAGAAAATATTTCTTGTCTAACCTCTTCTGTTCAATTGACAACTGC
ATTTTTTAAACTCAACAAAGTTTTATTTGCGCTACAACATCAATTTGATT
CACATTTCACAACCTTAATCTCAATCTCTATTACTCTTTATCTCTCTCTC
TCTCTCTTTTACAATTATTTTTACTCTTTTTAAACTTTATTACAAACATG
TGTTTCTTTATATTTTCTTAATAATTACAACATTATACCGTGTTTTCTGA
AAATAATATCTAGTGAACAATTTAAAATATTATTAAATATGAGCCCTATC
CGAAAATAAGCAATATGTAAAATTCTCAAATACCGAAACATCTCGCTTTG
TTGCGTCTTTTTATATTGAGCCCCCCAGACAAGGACATTAAGCATGCTAA
TTTAAAGATAGAAGGAATTGTTACAAAAAATTTAAAATTTAAGGGACTTT
GTTCAGAATTTTATTCTCTACAAAACTATTGGATTAGAATTTCAAATTTA
CGCAAATGGCGTACATAAAAATGCGATCATGAACTTTCTTTGTACTTATA
ATGTTCAAATAATTATCGGATCAAAATATGAGCTATGTGAATATTGTAGA
GAATTTGAAGCTCGTTAAGATTGTAATTTTGATTAGAATTTCTTTAAGGA
AATACCAGTTACATTGTTTATATTAATTTTGTTTGATTTTTGTTAGAAAA
AATGTAGAGTTAAGGATGTAGTAGCGGAAAATGAAAATCATTGTGATGTT
TTACTTTCTCAAAGTTGACAAAAGATAGTGTAACATTCATACACACATAT
GTGGTGTATTATACATACGCTCACACTAATCAACCAAATCCTTGCATTGC
TATTGCATTTGTATTGCGTATATAAGTATGAATATCATCAAAAATACTAA
AATTTGTTAAAACTTAATATTTAAAAATAAATAAATTGATATTTAAAATA
AAGTGATATAATATAAATTGATTGAGAATAAATATTCTAAGTTTATTTTA
CAACCTTTGATGTATAAAACTCTTGATAAGTGTGTTAATAACCTAAATGA
AATTGATGAAATAATTAACCTGATTTTGATTCGCTTAATTTCTTTAAGAA
TGGTCCTTTGATTAACACGACTAGTAATGTAATGAATATGCCTGATGATG
TTTAATTGCATAGTAATGAAAGCAAAAATGCTAGGTTTGACAAAATAATT
GAAAGTTTTACGGTTTGCTGAGAGTTTTAGGAACTTTGAAAAAGCTATTT
TAAATAATATTGGAAGTATCAATGTTAAAATTATTTCAAAGTATTTCAAA
GTTTGTGATGTGTTCAGTAATAAATATGTAAAATCCTTTTCGCATATGTT
TTGATCTCATATGTCGTTTTACTTGTAATGGCCGTAATAAAACATTCATT
GAAGAGACATCCCGTCATTTCTGGACACAAGTTTGAGAACATGCCCGAAT
TGAGTGAACACAGTAATATTATTGCGCATAATAAAACATGTAAGTTGAAA
ATTTATATTTCCAACTTTAAGTGTTATCTAACGGTTTCAGAAACTTTTTT
GAATATATTGCTTATGAAGCTTAGATAATAAAATCATTTTAACCAAAAGT
TAATATGTAAAATTCTATAGATTACATTTTGATTTTTAAATTAATGTACT
AGTGTTAATATATTTTAATAGAATCTGC
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protein sequence of SMED30003838-orf-1

>SMED30003838-orf-1 ID=SMED30003838-orf-1|Name=SMED30003838-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=195bp
TICIIKEFQFLIFLMDSKEVEERFFQLATPDLIRKWIKKFGIINLGIPND
INNQFENCNKFCTGKIKKTQFVEQGEIVDCKRKIQMIRGASNLEPIKVDS
TYDERRGSLQTMRQFNSLPSTTSNNLASLINSKITLPKFLQDENHLKCLR
ETNWTEFFVEIVSGVLSDLDYQSVINKIRTNMGILRIRITVSCT*
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protein sequence of SMED30003838-orf-2

>SMED30003838-orf-2 ID=SMED30003838-orf-2|Name=SMED30003838-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp
MHINDKLVGSSNFSPEIVNYFLQISRKCRKENKPQKYQNDEVPECLHSSG
IGELLCISLHLKSHDSVRPPKLSAALILINPTSSKNILQVSFSDFIHFNT
CIAPVYFANINFC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:00041143',5'-cyclic-nucleotide phosphodiesterase activity
GO:0008081phosphoric diester hydrolase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction