SMED30003777
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003777 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 41Note: Hover over icons to view figure legend
Alignments
SMED30003777 aligns in the following genomic locations:
Homology
BLAST of SMED30003777 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1) HSP 1 Score: 306.605 bits (784), Expect = 2.416e-94 Identity = 173/177 (97.74%), Postives = 174/177 (98.31%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIESTSTATKRLTTENXXXXXXXXXXXXXXXXXXXLSIVSSKSENR 774 CAIIDGLVSQ+LINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKN ESTSTATK LTTENITTKSSPSSKSTYIPTTTTL IVSSKSENR Sbjct: 3773 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNAESTSTATKHLTTENITTKSSPSSKSTYIPTTTTLLIVSSKSENR 3949 HSP 2 Score: 164.466 bits (415), Expect = 5.002e-45 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S +LP+F +DFTDNG+ E FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 4645 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 4778 HSP 3 Score: 163.696 bits (413), Expect = 1.183e-44 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFTDNG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 2031 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 2164 HSP 4 Score: 162.54 bits (410), Expect = 3.107e-44 Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFT+NG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 5637 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 5770 HSP 5 Score: 73.559 bits (179), Expect = 4.392e-14 Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQN-----MTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNI 642 C +I+GLVS++ I D Q+ +N K D RVS LR S+ G IS+ S F++DFT+NG RE FI +II + + N TF P V ++NK +K+ ++ L ++++ +KIQILGCF KN+ Sbjct: 6193 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF-KNV 6329 HSP 6 Score: 58.5362 bits (140), Expect = 3.579e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 4818 CTLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCF-KNI 4958 HSP 7 Score: 58.5362 bits (140), Expect = 4.056e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 2204 CKLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEREEVPSKRLVDLEVIGCF-KNI 2344
BLAST of SMED30003777 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1) HSP 1 Score: 306.605 bits (784), Expect = 2.416e-94 Identity = 173/177 (97.74%), Postives = 174/177 (98.31%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIESTSTATKRLTTENXXXXXXXXXXXXXXXXXXXLSIVSSKSENR 774 CAIIDGLVSQ+LINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKN ESTSTATK LTTENITTKSSPSSKSTYIPTTTTL IVSSKSENR Sbjct: 3754 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNAESTSTATKHLTTENITTKSSPSSKSTYIPTTTTLLIVSSKSENR 3930 HSP 2 Score: 164.466 bits (415), Expect = 5.002e-45 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S +LP+F +DFTDNG+ E FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 4626 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 4759 HSP 3 Score: 163.696 bits (413), Expect = 1.183e-44 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFTDNG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 2031 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 2164 HSP 4 Score: 162.54 bits (410), Expect = 3.107e-44 Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFT+NG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 5618 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 5751 HSP 5 Score: 73.559 bits (179), Expect = 4.392e-14 Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQN-----MTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNI 642 C +I+GLVS++ I D Q+ +N K D RVS LR S+ G IS+ S F++DFT+NG RE FI +II + + N TF P V ++NK +K+ ++ L ++++ +KIQILGCF KN+ Sbjct: 6193 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF-KNV 6329 HSP 6 Score: 58.5362 bits (140), Expect = 3.579e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 4799 CTLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCF-KNI 4939 HSP 7 Score: 56.225 bits (134), Expect = 1.815e-8 Identity = 29/74 (39.19%), Postives = 41/74 (55.41%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIIL 450 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ Sbjct: 2204 CKLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLII 2277
BLAST of SMED30003777 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1) HSP 1 Score: 306.605 bits (784), Expect = 2.416e-94 Identity = 173/177 (97.74%), Postives = 174/177 (98.31%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIESTSTATKRLTTENXXXXXXXXXXXXXXXXXXXLSIVSSKSENR 774 CAIIDGLVSQ+LINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKN ESTSTATK LTTENITTKSSPSSKSTYIPTTTTL IVSSKSENR Sbjct: 3756 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNAESTSTATKHLTTENITTKSSPSSKSTYIPTTTTLLIVSSKSENR 3932 HSP 2 Score: 164.851 bits (416), Expect = 4.954e-45 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S +LP+F +DFTDNG+ E FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 4628 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 4761 HSP 3 Score: 163.696 bits (413), Expect = 1.183e-44 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFTDNG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 2033 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 2166 HSP 4 Score: 162.54 bits (410), Expect = 3.107e-44 Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFT+NG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 5620 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 5753 HSP 5 Score: 73.559 bits (179), Expect = 4.312e-14 Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQN-----MTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNI 642 C +I+GLVS++ I D Q+ +N K D RVS LR S+ G IS+ S F++DFT+NG RE FI +II + + N TF P V ++NK +K+ ++ L ++++ +KIQILGCF KN+ Sbjct: 6195 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF-KNV 6331 HSP 6 Score: 58.5362 bits (140), Expect = 3.453e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 4801 CTLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCF-KNI 4941 HSP 7 Score: 56.225 bits (134), Expect = 1.783e-8 Identity = 29/74 (39.19%), Postives = 41/74 (55.41%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIIL 450 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ Sbjct: 2206 CKLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLII 2279
BLAST of SMED30003777 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1) HSP 1 Score: 306.605 bits (784), Expect = 2.416e-94 Identity = 173/177 (97.74%), Postives = 174/177 (98.31%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIESTSTATKRLTTENXXXXXXXXXXXXXXXXXXXLSIVSSKSENR 774 CAIIDGLVSQ+LINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKN ESTSTATK LTTENITTKSSPSSKSTYIPTTTTL IVSSKSENR Sbjct: 3775 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNAESTSTATKHLTTENITTKSSPSSKSTYIPTTTTLLIVSSKSENR 3951 HSP 2 Score: 164.851 bits (416), Expect = 4.954e-45 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S +LP+F +DFTDNG+ E FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 4647 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 4780 HSP 3 Score: 163.696 bits (413), Expect = 1.183e-44 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFTDNG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 2033 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 2166 HSP 4 Score: 162.54 bits (410), Expect = 3.107e-44 Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFT+NG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 5639 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 5772 HSP 5 Score: 73.559 bits (179), Expect = 4.312e-14 Identity = 53/138 (38.41%), Postives = 79/138 (57.25%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQN-----MTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNI 642 C +I+GLVS++ I D Q+ +N K D RVS LR S+ G IS+ S F++DFT+NG RE FI +II + + N TF P V ++NK +K+ ++ L ++++ +KIQILGCF KN+ Sbjct: 6195 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF-KNV 6331 HSP 6 Score: 58.5362 bits (140), Expect = 3.453e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 4820 CTLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCF-KNI 4960 HSP 7 Score: 58.5362 bits (140), Expect = 3.913e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 2206 CKLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEREEVPSKRLVDLEVIGCF-KNI 2346
BLAST of SMED30003777 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1) HSP 1 Score: 306.605 bits (784), Expect = 2.417e-94 Identity = 173/177 (97.74%), Postives = 174/177 (98.31%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIESTSTATKRLTTENXXXXXXXXXXXXXXXXXXXLSIVSSKSENR 774 CAIIDGLVSQ+LINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKN ESTSTATK LTTENITTKSSPSSKSTYIPTTTTL IVSSKSENR Sbjct: 3773 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNAESTSTATKHLTTENITTKSSPSSKSTYIPTTTTLLIVSSKSENR 3949 HSP 2 Score: 164.466 bits (415), Expect = 5.099e-45 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S +LP+F +DFTDNG+ E FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 4645 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 4778 HSP 3 Score: 163.696 bits (413), Expect = 1.217e-44 Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFTDNG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 2031 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 2164 HSP 4 Score: 162.155 bits (409), Expect = 3.198e-44 Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNIE 645 CAIIDGLVSQYLIND QI ++GK D RVS +R S G E+S + P+F +DFT+NG+RE FIYQ+I TGGV+T + D +++ ++K+EP V IN+NIKTL ++SF ++EKIFLKIQILGCFEK E Sbjct: 5637 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTE 5770 HSP 5 Score: 73.559 bits (179), Expect = 4.639e-14 Identity = 54/138 (39.13%), Postives = 82/138 (59.42%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILT---GGVKTANI--LTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCFEKNI 642 C +I+GLVS++ I D Q+ +N K D RVS LR S+ G IS+ S F++DFT+NG RE FI +II + G ++ I + TF P V ++NK +K+ ++ L ++++ +KIQILGCF KN+ Sbjct: 6212 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF-KNV 6348 HSP 6 Score: 58.5362 bits (140), Expect = 3.644e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 4818 CTLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCF-KNI 4958 HSP 7 Score: 58.5362 bits (140), Expect = 4.129e-9 Identity = 43/148 (29.05%), Postives = 74/148 (50.00%), Query Frame = 1 Query: 244 CAIIDGLVSQYLINDHQIMVNGKPDPRVSF-----LRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKE----------KIFLKIQILGCFEKNI 642 C +++G+VS I DHQI +N K D + LR S+ G EI+ +D TDNG RE FI+Q+I+ + + ++ +++ K V+Q ++ K +++ +L S E K + ++++GCF KNI Sbjct: 2204 CKLVNGMVSNVFIADHQISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRK----VYQSDE--KEFTIDVGEYLISVEIVIEREEVPSKRLVDLEVIGCF-KNI 2344 The following BLAST results are available for this feature:
BLAST of SMED30003777 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30003777 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30003777 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30003777 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30003777 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003777 ID=SMED30003777|Name=SMED30003777|organism=Schmidtea mediterranea sexual|type=transcript|length=958bpback to top protein sequence of SMED30003777-orf-1 >SMED30003777-orf-1 ID=SMED30003777-orf-1|Name=SMED30003777-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=319bp TKSSTTGPTTTTKSSTSSGTTTIPLSVSSTTLPPSVSTTSISTSSPTTKSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|