KH domain-containing protein

Overview
NameKH domain-containing protein
Smed IDSMED30003741
Length (bp)971
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of KH domain-containing protein (SMED30003741) t-SNE clustered cells

Violin plots show distribution of expression levels for KH domain-containing protein (SMED30003741) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of KH domain-containing protein (SMED30003741) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for KH domain-containing protein (SMED30003741) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003741

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30003741h1SMcG0011082 SmedASXL_006165SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30003741h1SMcG0011082 SmedASXL_006165SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30003741h1SMcG0011082 dd_Smed_v4_1393_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30003741h1SMcG0011082 dd_Smed_v4_1393_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003741h1SMcG0011082 dd_Smed_v4_1393_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003741h1SMcG0011082 dd_Smed_v4_1393_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003741h1SMcG0011082 dd_Smed_v4_1393_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
X1 cellSMED30003741h1SMcG0011082 isotig08321be_Smed_v2PMID:22543868
Onal et al., 2012
FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of KH domain-containing protein vs. Ensembl Human
Match: KHDRBS3 (KH RNA binding domain containing, signal transduction associated 3 [Source:HGNC Symbol;Acc:HGNC:18117])

HSP 1 Score: 85.5001 bits (210), Expect = 1.394e-19
Identity = 44/111 (39.64%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +ID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K              F   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   18 YIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 128          
BLAST of KH domain-containing protein vs. Ensembl Human
Match: KHDRBS2 (KH RNA binding domain containing, signal transduction associated 2 [Source:HGNC Symbol;Acc:HGNC:18114])

HSP 1 Score: 87.4261 bits (215), Expect = 7.079e-19
Identity = 49/135 (36.30%), Postives = 76/135 (56.30%), Query Frame = 3
Query:  204 SSLGASEIHRLRGGIAD-------FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            S L A EI + +G           ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  ++LK +QE+T  KM+I G          E  ++     + H +++LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   25 SRLLAEEIEKFQGSDGKKEDEEKKYLDVISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKAKEEELRKSGEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Human
Match: KHDRBS3 (KH RNA binding domain containing, signal transduction associated 3 [Source:HGNC Symbol;Acc:HGNC:18117])

HSP 1 Score: 85.8853 bits (211), Expect = 9.706e-19
Identity = 44/111 (39.64%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +ID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K              F   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   17 YIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 127          
BLAST of KH domain-containing protein vs. Ensembl Human
Match: KHDRBS3 (KH RNA binding domain containing, signal transduction associated 3 [Source:HGNC Symbol;Acc:HGNC:18117])

HSP 1 Score: 85.5001 bits (210), Expect = 3.099e-18
Identity = 44/111 (39.64%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +ID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K              F   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   45 YIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 155          
BLAST of KH domain-containing protein vs. Ensembl Human
Match: KHDRBS1 (KH RNA binding domain containing, signal transduction associated 1 [Source:HGNC Symbol;Acc:HGNC:18116])

HSP 1 Score: 80.1073 bits (196), Expect = 4.731e-16
Identity = 44/133 (33.08%), Postives = 77/133 (57.89%), Query Frame = 3
Query:  219 SEIHRLRGGIA------DFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDHTE-KLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNL 569
            +EI +++ G +      +++DL  +K +K++ ++ IP  QYP  NFVGK+LGP  +T+K +QE+T  K+++ G          E  ++   P + H    LH+ I+  G P EAY  ++HA+ EV+K L  ++
Sbjct:  127 AEIEKIQKGDSKKDDEENYLDLFSHKNMKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDM 259          
BLAST of KH domain-containing protein vs. Ensembl Celegans
Match: asd-2 (RNA-binding protein asd-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EFF1])

HSP 1 Score: 59.6918 bits (143), Expect = 6.633e-10
Identity = 33/95 (34.74%), Postives = 57/95 (60.00%), Query Frame = 3
Query:  297 KIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG---IENPKQKNM----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            K+ +PA ++P  NFVG++LGP   T K ++++T  K+ +RG   + + K++ +    P ++H +E+LH+ I    T   A  KL  A+ EV+K L
Sbjct:   91 KVFVPAKEHPDYNFVGRILGPRGMTAKQLEQETGCKIMVRGRGSMRDKKKEELNRGKPNWEHLSEELHVLIQCEDTENRAKVKLMRAVEEVRKLL 185          
BLAST of KH domain-containing protein vs. Ensembl Celegans
Match: asd-2 (RNA-binding protein asd-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EFF1])

HSP 1 Score: 59.3066 bits (142), Expect = 1.141e-9
Identity = 33/95 (34.74%), Postives = 57/95 (60.00%), Query Frame = 3
Query:  297 KIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG---IENPKQKNM----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            K+ +PA ++P  NFVG++LGP   T K ++++T  K+ +RG   + + K++ +    P ++H +E+LH+ I    T   A  KL  A+ EV+K L
Sbjct:   91 KVFVPAKEHPDYNFVGRILGPRGMTAKQLEQETGCKIMVRGRGSMRDKKKEELNRGKPNWEHLSEELHVLIQCEDTENRAKVKLMRAVEEVRKLL 185          
BLAST of KH domain-containing protein vs. Ensembl Celegans
Match: asd-2 (RNA-binding protein asd-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EFF1])

HSP 1 Score: 59.3066 bits (142), Expect = 1.194e-9
Identity = 33/95 (34.74%), Postives = 57/95 (60.00%), Query Frame = 3
Query:  297 KIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG---IENPKQKNM----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            K+ +PA ++P  NFVG++LGP   T K ++++T  K+ +RG   + + K++ +    P ++H +E+LH+ I    T   A  KL  A+ EV+K L
Sbjct:  133 KVFVPAKEHPDYNFVGRILGPRGMTAKQLEQETGCKIMVRGRGSMRDKKKEELNRGKPNWEHLSEELHVLIQCEDTENRAKVKLMRAVEEVRKLL 227          
BLAST of KH domain-containing protein vs. Ensembl Celegans
Match: asd-2 (RNA-binding protein asd-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EFF1])

HSP 1 Score: 59.3066 bits (142), Expect = 1.352e-9
Identity = 33/95 (34.74%), Postives = 57/95 (60.00%), Query Frame = 3
Query:  297 KIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG---IENPKQKNM----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            K+ +PA ++P  NFVG++LGP   T K ++++T  K+ +RG   + + K++ +    P ++H +E+LH+ I    T   A  KL  A+ EV+K L
Sbjct:  163 KVFVPAKEHPDYNFVGRILGPRGMTAKQLEQETGCKIMVRGRGSMRDKKKEELNRGKPNWEHLSEELHVLIQCEDTENRAKVKLMRAVEEVRKLL 257          
BLAST of KH domain-containing protein vs. Ensembl Celegans
Match: Y69A2AR.32 (pep chromosome:WBcel235:IV:2610408:2612430:1 gene:WBGene00022101.1 transcript:Y69A2AR.32a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y69A2AR.32)

HSP 1 Score: 54.6842 bits (130), Expect = 3.453e-8
Identity = 31/105 (29.52%), Postives = 55/105 (52.38%), Query Frame = 3
Query:  306 IPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG-----IENPKQ---KNMPVFDH------------TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLT 560
            +P ++YP  NFVG++LGP   T+K ++++T  ++ +RG       NP+    K+ P F               E LH+ I+   T + A  K++HA+  +Q+ L+
Sbjct:   77 VPVEKYPKYNFVGRILGPRGMTVKQLEKETGCRIFVRGRASTTASNPESKPNKSTPSFSKPSLSIISRNALTEEPLHVYIECQDTQSAAQAKMAHAVEVIQRLLS 181          
BLAST of KH domain-containing protein vs. Ensembl Fly
Match: qkr58E-3 (gene:FBgn0022984 transcript:FBtr0339538)

HSP 1 Score: 79.7221 bits (195), Expect = 1.342e-16
Identity = 39/115 (33.91%), Postives = 69/115 (60.00%), Query Frame = 3
Query:  243 GIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTEK-LHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            G   + D+   K +K+  K+ +P  QYP  NF GK+LGP  ++L+ +QE+T  K+ I+G  + + +N          P + H +K L L++ ++ TPAE Y ++++A+AE++K L
Sbjct:   65 GKEFYADVYKQKPMKITQKVFVPVKQYPKFNFTGKILGPKGNSLRRLQEETQCKIAIKGRSSIRDRNKEEQLRSTGDPRYAHLQKDLFLEVSTVATPAECYARIAYALAEIRKYL 179          
BLAST of KH domain-containing protein vs. Ensembl Fly
Match: qkr58E-3 (gene:FBgn0022984 transcript:FBtr0307214)

HSP 1 Score: 79.7221 bits (195), Expect = 1.342e-16
Identity = 39/115 (33.91%), Postives = 69/115 (60.00%), Query Frame = 3
Query:  243 GIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTEK-LHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            G   + D+   K +K+  K+ +P  QYP  NF GK+LGP  ++L+ +QE+T  K+ I+G  + + +N          P + H +K L L++ ++ TPAE Y ++++A+AE++K L
Sbjct:   65 GKEFYADVYKQKPMKITQKVFVPVKQYPKFNFTGKILGPKGNSLRRLQEETQCKIAIKGRSSIRDRNKEEQLRSTGDPRYAHLQKDLFLEVSTVATPAECYARIAYALAEIRKYL 179          
BLAST of KH domain-containing protein vs. Ensembl Fly
Match: qkr58E-3 (gene:FBgn0022984 transcript:FBtr0290254)

HSP 1 Score: 79.7221 bits (195), Expect = 1.342e-16
Identity = 39/115 (33.91%), Postives = 69/115 (60.00%), Query Frame = 3
Query:  243 GIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTEK-LHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            G   + D+   K +K+  K+ +P  QYP  NF GK+LGP  ++L+ +QE+T  K+ I+G  + + +N          P + H +K L L++ ++ TPAE Y ++++A+AE++K L
Sbjct:   65 GKEFYADVYKQKPMKITQKVFVPVKQYPKFNFTGKILGPKGNSLRRLQEETQCKIAIKGRSSIRDRNKEEQLRSTGDPRYAHLQKDLFLEVSTVATPAECYARIAYALAEIRKYL 179          
BLAST of KH domain-containing protein vs. Ensembl Fly
Match: CG3927 (gene:FBgn0034739 transcript:FBtr0071853)

HSP 1 Score: 75.485 bits (184), Expect = 2.374e-15
Identity = 37/115 (32.17%), Postives = 69/115 (60.00%), Query Frame = 3
Query:  243 GIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTEK-LHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            G   F D+   K +K+  K+ +P +++P  NF GK+LGP  ++L+ +QE+T+ K+ I+G  + + +N          P + H  K L L++ ++  PAE Y ++++A+AE++K L
Sbjct:   66 GKEPFADVYQQKPMKIIQKVFVPVNKFPKFNFTGKILGPKGNSLRRLQEETHCKIVIKGRNSMRDRNKEEELRSSGDPRYAHLHKDLFLEVSAVAPPAECYARIAYALAEIRKYL 180          
BLAST of KH domain-containing protein vs. Ensembl Fly
Match: CG4021 (gene:FBgn0034659 transcript:FBtr0071716)

HSP 1 Score: 75.8702 bits (185), Expect = 2.821e-15
Identity = 35/109 (32.11%), Postives = 68/109 (62.39%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM-------PVFDHTEK-LHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            + D+   K +K+  K+ +P +QYP  NF GK+LGP  ++L+ +QE+T  K+ ++G  + + +N        P + H +K L L++ ++  PAE + ++++A+AE++K L
Sbjct:   73 YADVYRQKPMKIIQKVFVPVNQYPKFNFAGKILGPKGNSLRRLQEETQCKIALKGRSSMRDRNKEEELRSDPRYAHLQKNLFLEVSTVAIPAECHSRIAYALAEIRKYL 181          
BLAST of KH domain-containing protein vs. Ensembl Zebrafish
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:768147])

HSP 1 Score: 87.0409 bits (214), Expect = 6.025e-19
Identity = 53/161 (32.92%), Postives = 82/161 (50.93%), Query Frame = 3
Query:  105 PELKISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            PEL   K  +D   + A++   E I       L         R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I G          E  ++     + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:    8 PELVAEKESLDASFVHAMRLLAEEIEKFEGDEL---------RKDGEVKKYLDIISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKGKEEELRKSGEAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Zebrafish
Match: khdrbs1a (KH domain containing, RNA binding, signal transduction associated 1a [Source:ZFIN;Acc:ZDB-GENE-000210-25])

HSP 1 Score: 79.337 bits (194), Expect = 3.452e-16
Identity = 49/153 (32.03%), Postives = 84/153 (54.90%), Query Frame = 3
Query:  144 QIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIAD-----FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTE-KLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +++A ++ L+   T    +LS+    EI R++ G        ++DL  +K V+V+ ++ IP  QYP  NFVGK+LGP  ST+K +QE T  K+++ G  + + KN          P + H   +LH+ I+      + Y +++HA+ EV+K L
Sbjct:   20 ELLAEKDSLDSSFTHAMKLLSA----EIERVQKGEPKKDSELYLDLFASKSVRVKERVLIPVKQYPRFNFVGKILGPQGSTIKRLQEDTGAKISVLGKGSMRDKNKEEELRKGGDPKYAHLGMELHVHIEVFAPIPDCYLRMAHAMDEVKKFL 168          
BLAST of KH domain-containing protein vs. Ensembl Zebrafish
Match: khdrbs1a (KH domain containing, RNA binding, signal transduction associated 1a [Source:ZFIN;Acc:ZDB-GENE-000210-25])

HSP 1 Score: 79.337 bits (194), Expect = 3.745e-16
Identity = 49/153 (32.03%), Postives = 84/153 (54.90%), Query Frame = 3
Query:  144 QIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIAD-----FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTE-KLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +++A ++ L+   T    +LS+    EI R++ G        ++DL  +K V+V+ ++ IP  QYP  NFVGK+LGP  ST+K +QE T  K+++ G  + + KN          P + H   +LH+ I+      + Y +++HA+ EV+K L
Sbjct:   20 ELLAEKDSLDSSFTHAMKLLSA----EIERVQKGEPKKDSELYLDLFASKSVRVKERVLIPVKQYPRFNFVGKILGPQGSTIKRLQEDTGAKISVLGKGSMRDKNKEEELRKGGDPKYAHLGMELHVHIEVFAPIPDCYLRMAHAMDEVKKFL 168          
BLAST of KH domain-containing protein vs. Ensembl Zebrafish
Match: khdrbs1a (KH domain containing, RNA binding, signal transduction associated 1a [Source:ZFIN;Acc:ZDB-GENE-000210-25])

HSP 1 Score: 78.9518 bits (193), Expect = 4.091e-16
Identity = 49/153 (32.03%), Postives = 84/153 (54.90%), Query Frame = 3
Query:  144 QIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIAD-----FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTE-KLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +++A ++ L+   T    +LS+    EI R++ G        ++DL  +K V+V+ ++ IP  QYP  NFVGK+LGP  ST+K +QE T  K+++ G  + + KN          P + H   +LH+ I+      + Y +++HA+ EV+K L
Sbjct:   20 ELLAEKDSLDSSFTHAMKLLSA----EIERVQKGEPKKDSELYLDLFASKSVRVKERVLIPVKQYPRFNFVGKILGPQGSTIKRLQEDTGAKISVLGKGSMRDKNKEEELRKGGDPKYAHLGMELHVHIEVFAPIPDCYLRMAHAMDEVKKFL 168          
BLAST of KH domain-containing protein vs. Ensembl Zebrafish
Match: khdrbs1a (KH domain containing, RNA binding, signal transduction associated 1a [Source:ZFIN;Acc:ZDB-GENE-000210-25])

HSP 1 Score: 78.9518 bits (193), Expect = 4.091e-16
Identity = 49/153 (32.03%), Postives = 84/153 (54.90%), Query Frame = 3
Query:  144 QIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIAD-----FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDHTE-KLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +++A ++ L+   T    +LS+    EI R++ G        ++DL  +K V+V+ ++ IP  QYP  NFVGK+LGP  ST+K +QE T  K+++ G  + + KN          P + H   +LH+ I+      + Y +++HA+ EV+K L
Sbjct:   20 ELLAEKDSLDSSFTHAMKLLSA----EIERVQKGEPKKDSELYLDLFASKSVRVKERVLIPVKQYPRFNFVGKILGPQGSTIKRLQEDTGAKISVLGKGSMRDKNKEEELRKGGDPKYAHLGMELHVHIEVFAPIPDCYLRMAHAMDEVKKFL 168          
BLAST of KH domain-containing protein vs. Ensembl Xenopus
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:779662])

HSP 1 Score: 84.3445 bits (207), Expect = 8.476e-18
Identity = 43/115 (37.39%), Postives = 69/115 (60.00%), Query Frame = 3
Query:  243 GIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            G   ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  ++LK +QE+T  KM+I G          E  ++ +     H +++LH+ ++    P EAY ++SHA+ E++K L
Sbjct:   45 GEKKYLDIISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKIKEEELRKSDEAKHAHLSDELHVLLEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Xenopus
Match: KHDRBS3 (KH domain containing, RNA binding, signal transduction associated 3 [Source:NCBI gene;Acc:100379996])

HSP 1 Score: 83.1889 bits (204), Expect = 1.653e-17
Identity = 47/126 (37.30%), Postives = 73/126 (57.94%), Query Frame = 3
Query:  222 EIHRLRGGIAD----FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            EI +L+ G  +    +ID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE T TKM+I G  + + K              +   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   29 EIEKLQKGEDNTEDQYIDVVINKNMKLAQKVLIPIKQFPKFNFVGKLLGPRGNSLKRLQEDTLTKMSILGKGSMRDKAKEEELRKSGEAKYYHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 154          
BLAST of KH domain-containing protein vs. Ensembl Xenopus
Match: pdc (phosducin [Source:Xenbase;Acc:XB-GENE-983832])

HSP 1 Score: 82.8037 bits (203), Expect = 3.153e-17
Identity = 52/146 (35.62%), Postives = 80/146 (54.79%), Query Frame = 3
Query:  207 SLGASEIHRLR-GGIAD------FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDHTE-KLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVD 593
            SL   EI RL+ GG A       ++DL  +K +K++ +I IP   YP  NFVGK+LGP  +T+K +QE+T  K+++ G          E  ++   P + H    LH+ I+  G P E+Y +++HA+ EV+K L    P S+   D
Sbjct:   27 SLLGKEIERLKKGGDAKKDEEDTYLDLFSHKNMKLKERILIPVKLYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYSHLNMDLHVFIEVFGPPCESYTRMAHAMEEVKKFLVPLTPESFSYQD 172          
BLAST of KH domain-containing protein vs. Ensembl Xenopus
Match: pdc (phosducin [Source:Xenbase;Acc:XB-GENE-983832])

HSP 1 Score: 82.0333 bits (201), Expect = 6.164e-17
Identity = 49/138 (35.51%), Postives = 78/138 (56.52%), Query Frame = 3
Query:  207 SLGASEIHRLR-GGIAD------FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDHTE-KLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNL 569
            SL   EI RL+ GG A       ++DL  +K +K++ +I IP   YP  NFVGK+LGP  +T+K +QE+T  K+++ G          E  ++   P + H    LH+ I+  G P E+Y +++HA+ EV+K L  ++
Sbjct:   53 SLLGKEIERLKKGGDAKKDEEDTYLDLFSHKNMKLKERILIPVKLYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYSHLNMDLHVFIEVFGPPCESYTRMAHAMEEVKKFLVPDM 190          
BLAST of KH domain-containing protein vs. Ensembl Xenopus
Match: spsb3 (splA/ryanodine receptor domain and SOCS box containing 3 [Source:Xenbase;Acc:XB-GENE-971062])

HSP 1 Score: 56.225 bits (134), Expect = 1.711e-8
Identity = 42/135 (31.11%), Postives = 64/135 (47.41%), Query Frame = 3
Query:  282 VKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK-------NMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVDPNNQNAVLVSTEYNMAPRVYTTP 662
            V+++ K+ +P  +YP  NFVG++LGP   T K ++ +T  K+ +RG  + + K         P ++H  E LH+ I        A  KL  A+ EV+K L        P V        L +T    APR+ T P
Sbjct:   81 VQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLV-------PAVSITALAFSLAATA--QAPRIITGP 206          
BLAST of KH domain-containing protein vs. Ensembl Mouse
Match: Khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:MGI Symbol;Acc:MGI:2159649])

HSP 1 Score: 87.0409 bits (214), Expect = 5.962e-19
Identity = 49/135 (36.30%), Postives = 76/135 (56.30%), Query Frame = 3
Query:  204 SSLGASEIHRLRGGIAD-------FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            S L A EI + +G           ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  ++LK +QE+T  KM+I G          E  ++     + H +++LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   25 SRLLAEEIEKFQGSDGKKEDEEKKYLDVISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKTKEEELRKSGEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Mouse
Match: Khdrbs3 (KH domain containing, RNA binding, signal transduction associated 3 [Source:MGI Symbol;Acc:MGI:1313312])

HSP 1 Score: 85.5001 bits (210), Expect = 2.087e-18
Identity = 44/111 (39.64%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +ID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K              F   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   45 YIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 155          
BLAST of KH domain-containing protein vs. Ensembl Mouse
Match: Khdrbs3 (KH domain containing, RNA binding, signal transduction associated 3 [Source:MGI Symbol;Acc:MGI:1313312])

HSP 1 Score: 85.5001 bits (210), Expect = 2.087e-18
Identity = 44/111 (39.64%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +ID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K              F   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   45 YIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 155          
BLAST of KH domain-containing protein vs. Ensembl Mouse
Match: Khdrbs3 (KH domain containing, RNA binding, signal transduction associated 3 [Source:MGI Symbol;Acc:MGI:1313312])

HSP 1 Score: 85.5001 bits (210), Expect = 2.087e-18
Identity = 44/111 (39.64%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +ID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K              F   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   45 YIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 155          
BLAST of KH domain-containing protein vs. Ensembl Mouse
Match: Khdrbs3 (KH domain containing, RNA binding, signal transduction associated 3 [Source:MGI Symbol;Acc:MGI:1313312])

HSP 1 Score: 75.0998 bits (183), Expect = 3.006e-16
Identity = 40/102 (39.22%), Postives = 59/102 (57.84%), Query Frame = 3
Query:  282 VKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQK----------NMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K              F   + LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:    1 MKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLNDDLHVLIEVFAPPAEAYARMGHALEEIKKFL 102          
BLAST of KH domain-containing protein vs. UniProt/SwissProt
Match: sp|P13230|GRP33_ARTSA (Glycine-rich protein GRP33 OS=Artemia salina OX=85549 PE=2 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.944e-19
Identity = 47/127 (37.01%), Postives = 80/127 (62.99%), Query Frame = 3
Query:  249 ADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNMP---------VFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVDPN 599
            + F+DL  + KVK+ ++  +P DQ+P  NF+GKLLGPG ST+K +Q++T TK++I G  + + +N            + H  E+LH++I S+ +PAEA+ ++++A+ E++K +T       P  DPN
Sbjct:   61 SGFMDLYNDTKVKLVSRCCLPVDQFPKYNFLGKLLGPGGSTMKQLQDETMTKISILGRGSMRDRNKEEELRNSGDVKYAHLNEQLHIEIISIASPAEAHARMAYALTEIKKYIT-------PEEDPN 180          
BLAST of KH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5VWX1|KHDR2_HUMAN (KH domain-containing, RNA-binding, signal transduction-associated protein 2 OS=Homo sapiens OX=9606 GN=KHDRBS2 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.400e-18
Identity = 49/135 (36.30%), Postives = 76/135 (56.30%), Query Frame = 3
Query:  204 SSLGASEIHRLRGGIAD-------FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            S L A EI + +G           ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  ++LK +QE+T  KM+I G          E  ++     + H +++LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   25 SRLLAEEIEKFQGSDGKKEDEEKKYLDVISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKAKEEELRKSGEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q920F3|KHDR2_RAT (KH domain-containing, RNA-binding, signal transduction-associated protein 2 OS=Rattus norvegicus OX=10116 GN=Khdrbs2 PE=1 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.711e-18
Identity = 49/135 (36.30%), Postives = 76/135 (56.30%), Query Frame = 3
Query:  204 SSLGASEIHRLRGGIA-------DFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            S L A EI + +G           ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  ++LK +QE+T  KM+I G          E  ++     + H +++LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   25 SRLLAEEIEKFQGSDGRKEDEEKKYLDVISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKAKEEELRKSGEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9WU01|KHDR2_MOUSE (KH domain-containing, RNA-binding, signal transduction-associated protein 2 OS=Mus musculus OX=10090 GN=Khdrbs2 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.170e-18
Identity = 49/135 (36.30%), Postives = 76/135 (56.30%), Query Frame = 3
Query:  204 SSLGASEIHRLRGGIAD-------FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            S L A EI + +G           ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  ++LK +QE+T  KM+I G          E  ++     + H +++LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   25 SRLLAEEIEKFQGSDGKKEDEEKKYLDVISNKNIKLSERVLIPVKQYPKFNFVGKLLGPRGNSLKRLQEETGAKMSILGKGSMRDKTKEEELRKSGEAKYAHLSDELHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q08BJ2|KHDR2_DANRE (KH domain-containing, RNA-binding, signal transduction-associated protein 2 OS=Danio rerio OX=7955 GN=khdrbs2 PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.548e-18
Identity = 53/161 (32.92%), Postives = 82/161 (50.93%), Query Frame = 3
Query:  105 PELKISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            PEL   K  +D   + A++   E I       L         R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I G          E  ++     + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:    8 PELVAEKESLDASFVHAMRLLAEEIEKFEGDEL---------RKDGEVKKYLDIISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKGKEEELRKSGEAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. TrEMBL
Match: A4V6L0 (Sam68-like mammalian protein 1 (Fragment) OS=Dugesia japonica OX=6161 PE=2 SV=1)

HSP 1 Score: 369.392 bits (947), Expect = 7.445e-126
Identity = 188/243 (77.37%), Postives = 210/243 (86.42%), Query Frame = 3
Query:  114 KISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVDPNNQNAVLVSTEYNMAPRVYTTPXXXXXXXXXXXXXALRVSPRFVPPPAPTEWLNPEEANGGQELISGGPIHNEKNLNRITPY 842
            KISK FIDDQQIIAIQ+EL++I+ LNCPILS+L A EI RLRGGIAD+IDLILNKKVKVR K+DIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI+N K+  + + D  EKLHLQIDSLGTPAEAYYKLSHA+AEVQKCLTFNLP SYPTVDPNNQNAV + TEYNM PR Y +P+RG+QRGG RRG   R +PRFVPPPAP EW+ PEE NG Q++  GGPIH E+N +RI PY
Sbjct:    7 KISKSFIDDQQIIAIQSELDLIKDLNCPILSNLAAFEIDRLRGGIADYIDLILNKKVKVRAKVDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIDNQKKNAISIADQCEKLHLQIDSLGTPAEAYYKLSHAVAEVQKCLTFNLPLSYPTVDPNNQNAVQMVTEYNMTPRAYPSPVRGLQRGGARRGTVGRGNPRFVPPPAPAEWVKPEEPNGEQDISFGGPIHTERNPSRIAPY 249          
BLAST of KH domain-containing protein vs. TrEMBL
Match: A0A504Y6P7 (KH domain containing RNA binding signal OS=Fasciola gigantica OX=46835 GN=FGIG_01542 PE=4 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 3.339e-38
Identity = 77/160 (48.12%), Postives = 107/160 (66.88%), Query Frame = 3
Query:  111 LKISKPFIDDQQII-AIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI----ENPKQKNM-----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +K+ +P  +    + AI  EL+ +     P L +L  +E+ RLRGGIA+ IDL+ N++VK+R KIDIP  QYP  NFVGKLLGPG STL+++QE T TKM I G     +  K++ +     P + H  +KLHLQ+DSL  PAEAYY+LSHA+AE++K +
Sbjct:    1 MKMGEPRPEQSAAVEAIVRELKNLDETAFPALHALAENELFRLRGGIAESIDLVSNRQVKIRVKIDIPQTQYPNVNFVGKLLGPGGSTLRAVQESTKTKMAILGAGSLRDEAKERELLSNGDPKYQHLKQKLHLQVDSLAPPAEAYYRLSHALAELRKIM 160          
BLAST of KH domain-containing protein vs. TrEMBL
Match: A0A2H1C8T8 (KH domain containing RNA binding signal OS=Fasciola hepatica OX=6192 GN=D915_006012 PE=4 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 3.339e-38
Identity = 77/160 (48.12%), Postives = 107/160 (66.88%), Query Frame = 3
Query:  111 LKISKPFIDDQQII-AIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI----ENPKQKNM-----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +K+ +P  +    + AI  EL+ +     P L +L  +E+ RLRGGIA+ IDL+ N++VK+R KIDIP  QYP  NFVGKLLGPG STL+++QE T TKM I G     +  K++ +     P + H  +KLHLQ+DSL  PAEAYY+LSHA+AE++K +
Sbjct:    1 MKMGEPRPEQSAAVEAIVRELKNLDETAFPALHALAENELFRLRGGIAESIDLVSNRQVKIRVKIDIPQTQYPNVNFVGKLLGPGGSTLRAVQESTKTKMAILGAGSLRDEAKERELLSNGDPKYQHLKQKLHLQVDSLAPPAEAYYRLSHALAELRKIM 160          
BLAST of KH domain-containing protein vs. TrEMBL
Match: A0A448X8Z0 (KH domain-containing protein (Fragment) OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS24519 PE=4 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 7.606e-37
Identity = 71/135 (52.59%), Postives = 94/135 (69.63%), Query Frame = 3
Query:  165 ELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI----ENPKQKNM-----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIA 539
            ELE +     P L +L  SE+ RLRGGIAD I+++ NK+VK+R K++IP +QYP  NFVGKLLGPG  TL+SIQE+T TKM I G     +  K+K +     P + H  ++LHLQIDSL TP E+YY+LSHA++
Sbjct:   23 ELESLDQNAFPALHALAESELFRLRGGIADSIEIVDNKRVKIRAKVNIPTEQYPYINFVGKLLGPGGQTLRSIQEETKTKMAILGSGSLRDETKEKELLSNGDPKYQHLKQRLHLQIDSLATPVESYYRLSHALS 157          
BLAST of KH domain-containing protein vs. TrEMBL
Match: C1L818 (KH domain-containing, RNA-binding, signal transduction-associated protein 1 OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 7.669e-37
Identity = 75/147 (51.02%), Postives = 101/147 (68.71%), Query Frame = 3
Query:  147 IIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI----ENPKQKNM-----PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            + A+  EL+ + + + P L SL  +E+ RLRGGIAD  DL+ NK VKVR KI+IP  QYPT NFVGKLLGPG  TL+++QE T TKM I G     +  K+K +     P + H  +KLHLQ+DSLG P+E+ Y+L+HA+AEV+K +
Sbjct:   14 VEALVRELQNLDSNSFPALYSLAENELFRLRGGIADSFDLVSNKPVKVRAKIEIPQAQYPTINFVGKLLGPGGQTLRAVQETTKTKMAILGAGSLRDEAKEKQLLSNGDPKYQHLKQKLHLQVDSLGPPSESCYRLAHALAEVRKIM 160          
BLAST of KH domain-containing protein vs. Ensembl Cavefish
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:768147])

HSP 1 Score: 85.5001 bits (210), Expect = 1.592e-18
Identity = 44/119 (36.97%), Postives = 69/119 (57.98%), Query Frame = 3
Query:  231 RLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I G          E  ++     + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   41 RKDGEVKKYLDVISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKGKEEELRKSGEAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Cavefish
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:768147])

HSP 1 Score: 85.8853 bits (211), Expect = 2.360e-18
Identity = 44/119 (36.97%), Postives = 69/119 (57.98%), Query Frame = 3
Query:  231 RLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I G          E  ++     + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   41 RKDGEVKKYLDVISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKGKEEELRKSGEAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Cavefish
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:768147])

HSP 1 Score: 85.5001 bits (210), Expect = 2.376e-18
Identity = 44/119 (36.97%), Postives = 69/119 (57.98%), Query Frame = 3
Query:  231 RLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I G          E  ++     + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   41 RKDGEVKKYLDVISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKGKEEELRKSGEAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Cavefish
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:768147])

HSP 1 Score: 85.8853 bits (211), Expect = 2.607e-18
Identity = 44/119 (36.97%), Postives = 69/119 (57.98%), Query Frame = 3
Query:  231 RLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I G          E  ++     + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   41 RKDGEVKKYLDVISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKGKEEELRKSGEAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Cavefish
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:768147])

HSP 1 Score: 85.1149 bits (209), Expect = 2.997e-18
Identity = 44/119 (36.97%), Postives = 69/119 (57.98%), Query Frame = 3
Query:  231 RLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I G          E  ++     + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   41 RKDGEVKKYLDVISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKGKEEELRKSGEAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Sea Lamprey
Match: khdrbs2 (KH domain containing, RNA binding, signal transduction associated 2 [Source:NCBI gene;Acc:768147])

HSP 1 Score: 88.5817 bits (218), Expect = 2.534e-20
Identity = 46/111 (41.44%), Postives = 71/111 (63.96%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNMP---------VFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            F+DL+ NK +K+  ++ IPA Q+P  NFVGKLLGP  ++LK +QE+T TKM+I G  + + K +           + H +E+LH+ I+    P+EAY ++ HA+ EV+K L
Sbjct:   26 FLDLVSNKNMKLAERVIIPAKQHPKFNFVGKLLGPRGNSLKRMQEETGTKMSILGKGSMRDKALEEEMRKSGENKYAHLSEELHVLIEVFAPPSEAYSRMGHALDEVKKYL 136          
BLAST of KH domain-containing protein vs. Ensembl Sea Lamprey
Match: KHDRBS3 (KH RNA binding domain containing, signal transduction associated 3 [Source:HGNC Symbol;Acc:HGNC:18117])

HSP 1 Score: 80.8777 bits (198), Expect = 5.405e-18
Identity = 41/112 (36.61%), Postives = 68/112 (60.71%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLT 560
            FID++ N+ VK+  ++ IP  Q+P  NF+GKLLGP  ++LK ++E+T TK++I G          E+ ++     + H  E LH+ I+    P++AY ++ HA+ EV+K L 
Sbjct:   26 FIDVVSNRNVKLIERVIIPVRQHPKFNFIGKLLGPRGNSLKRLKEETGTKLSILGKGSMRDRDKEEDLRKSGEAKYAHLNEDLHVLIEVFAPPSQAYRRMGHALDEVKKYLV 137          
BLAST of KH domain-containing protein vs. Ensembl Sea Lamprey
Match: khdrbs1a (KH domain containing, RNA binding, signal transduction associated 1a [Source:ZFIN;Acc:ZDB-GENE-000210-25])

HSP 1 Score: 69.707 bits (169), Expect = 1.208e-13
Identity = 48/131 (36.64%), Postives = 69/131 (52.67%), Query Frame = 3
Query:  210 LGASEIHRLRGG-------IADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQK 551
            LGA EI RL+ G          + ++I  +  K+  K+ IPA Q+P  NFVGKLLGP  +TLK +Q +T T+M I G          E  +      + H  E+LH+ I+      EAY ++ HA+ EV+K
Sbjct:   29 LGA-EISRLQNGGDDKKDDDKKYRNIIGEQNFKLAEKVLIPAKQHPKFNFVGKLLGPQGNTLKRLQVETGTRMAIMGKGCMKDRAKEEELRNSGEAKYVHLNEELHVLIEVYAPLPEAYSRMGHALEEVKK 158          
BLAST of KH domain-containing protein vs. Ensembl Sea Lamprey
Match: sf1 (splicing factor 1 [Source:ZFIN;Acc:ZDB-GENE-030131-2492])

HSP 1 Score: 48.9062 bits (115), Expect = 9.136e-7
Identity = 20/45 (44.44%), Postives = 32/45 (71.11%), Query Frame = 3
Query:  285 KVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG 419
            K+  +I IP D++P  NF+G ++GP  +TLK I+++ N K+ IRG
Sbjct:  205 KISERIMIPQDEHPDINFIGLIIGPRGNTLKKIEKECNAKIMIRG 249          
BLAST of KH domain-containing protein vs. Ensembl Yeast
Match: MSL5 (Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000004106])

HSP 1 Score: 55.0694 bits (131), Expect = 6.231e-9
Identity = 25/48 (52.08%), Postives = 31/48 (64.58%), Query Frame = 3
Query:  276 KKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG 419
            +  K + K  IP DQYP  NFVG LLGP   TL+ +QE +N K+ IRG
Sbjct:  144 RPTKFQDKYYIPVDQYPDVNFVGLLLGPRGRTLRKLQEDSNCKIAIRG 191          
BLAST of KH domain-containing protein vs. Ensembl Nematostella
Match: EDO44127 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWI4])

HSP 1 Score: 65.855 bits (159), Expect = 4.539e-13
Identity = 41/106 (38.68%), Postives = 58/106 (54.72%), Query Frame = 3
Query:  270 LNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            L K VK+  K+ IP   +P  NFVGKLLGP  +T K +Q  T TKM+I G  + + K           P + H  E+LH+ I+    P +A+ +L  AI E++K L
Sbjct:   43 LYKPVKLSEKVFIPVKDHPKFNFVGKLLGPRGNTFKRLQNSTGTKMSILGKGSMRDKEKEEELRATEDPKYAHLGEELHVLIEVEAPPGQAHARLGIAIEEIKKYL 148          
BLAST of KH domain-containing protein vs. Ensembl Nematostella
Match: EDO30297 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T170])

HSP 1 Score: 57.3806 bits (137), Expect = 5.103e-10
Identity = 32/101 (31.68%), Postives = 52/101 (51.49%), Query Frame = 3
Query:  279 KVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM-------PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            +VK+  K+  P  ++P  NFVG+++GP   TL+ I+  T  K+ +RG  + K K +       P ++H  E LH+ I    T    + +L  A+  VQ  L
Sbjct:   50 RVKLVEKVYAPVKEFPKFNFVGRVIGPRGMTLREIESTTGCKLLVRGKGSMKDKKLEEEKRGQPNYEHLDEDLHVLISCEDTEERCHTRLEKAVERVQSLL 150          
BLAST of KH domain-containing protein vs. Ensembl Nematostella
Match: EDO45484 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSV9])

HSP 1 Score: 51.6026 bits (122), Expect = 1.187e-7
Identity = 30/83 (36.14%), Postives = 51/83 (61.45%), Query Frame = 3
Query:  282 VKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRG---IENPKQ---KNMPVFDHTEKLHLQIDSLGTPAEA 512
            +K++ K+ IP D+ P  NF+G L+GP  +TLK+++++TN K+ IRG   I++ KQ   ++M + +  E   L     G  AE+
Sbjct:  178 IKIQDKVMIPQDENPEVNFIGLLIGPRGNTLKNMEKETNAKIMIRGKGSIKDGKQGRGRDMQLMNSGEDEPLHALVTGPTAES 260          
BLAST of KH domain-containing protein vs. Ensembl Nematostella
Match: EDO28641 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5W9])

HSP 1 Score: 46.595 bits (109), Expect = 1.476e-6
Identity = 20/56 (35.71%), Postives = 34/56 (60.71%), Query Frame = 3
Query:  279 KVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM 446
            +VK+  K+  P  ++P  NFVG+++GP   TL+ I+  T  K+ +RG  + K K +
Sbjct:   89 RVKLVEKVYAPVKEFPKFNFVGRVIGPRGMTLREIESTTGCKLLVRGKGSMKDKKL 144          
BLAST of KH domain-containing protein vs. Ensembl Medaka
Match: KHDRBS3 (KH RNA binding domain containing, signal transduction associated 3 [Source:NCBI gene;Acc:101171680])

HSP 1 Score: 87.8113 bits (216), Expect = 2.457e-19
Identity = 47/111 (42.34%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            FID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE T TKM+I G          E  +Q     + H  E LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   46 FIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEDTLTKMSILGKGSMRDKEKEEELRQSGEAKYQHLNEDLHVLIEVFAPPAEAYARMGHALEEIKKFL 156          
BLAST of KH domain-containing protein vs. Ensembl Medaka
Match: KHDRBS3 (KH RNA binding domain containing, signal transduction associated 3 [Source:NCBI gene;Acc:101171680])

HSP 1 Score: 87.8113 bits (216), Expect = 2.779e-19
Identity = 47/111 (42.34%), Postives = 67/111 (60.36%), Query Frame = 3
Query:  255 FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---------ENPKQKNMPVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            FID+++NK +K+  K+ IP  Q+P  NFVGKLLGP  ++LK +QE T TKM+I G          E  +Q     + H  E LH+ I+    PAEAY ++ HA+ E++K L
Sbjct:   46 FIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEDTLTKMSILGKGSMRDKEKEEELRQSGEAKYQHLNEDLHVLIEVFAPPAEAYARMGHALEEIKKFL 156          
BLAST of KH domain-containing protein vs. Ensembl Medaka
Match: khdrbs2 (KH RNA binding domain containing, signal transduction associated 2 [Source:NCBI gene;Acc:101155877])

HSP 1 Score: 85.8853 bits (211), Expect = 1.367e-18
Identity = 54/161 (33.54%), Postives = 85/161 (52.80%), Query Frame = 3
Query:  105 PELKISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTI--RGIENPKQKNMPV-------FDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            PEL   K  +D   + A++   E I   +   L         R  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I  +G    K+K   +       + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:    8 PELVAEKNSLDPSFVHAVRLLAEEIEKYDGDEL---------RKDGDVKKYLDIISNKNIKLSERVLIPVRQYPKFNFVGKLLGPRGNSMKRLQEETGVKMSILGKGSMRDKEKEEELRKGGDAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 159          
BLAST of KH domain-containing protein vs. Ensembl Medaka
Match: khdrbs2 (KH RNA binding domain containing, signal transduction associated 2 [Source:NCBI gene;Acc:101155877])

HSP 1 Score: 85.8853 bits (211), Expect = 1.464e-18
Identity = 48/140 (34.29%), Postives = 81/140 (57.86%), Query Frame = 3
Query:  174 IIRTLNCPILSSLGASEIHRLR--GGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTI--RGIENPKQKNMPV-------FDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCL 557
            I  +++  +L+ +   +   LR  G +  ++D+I NK +K+  ++ IP  QYP  NFVGKLLGP  +++K +QE+T  KM+I  +G    K+K   +       + H +  LH+ I+    P EAY ++SHA+ E++K L
Sbjct:   23 IHSSIHGKVLTEIEKYDGDELRKDGDVKKYLDIISNKNIKLSERVLIPVRQYPKFNFVGKLLGPRGNSMKRLQEETGVKMSILGKGSMRDKEKEEELRKGGDAKYAHLSNDLHVLIEVFAPPGEAYSRMSHALEEIKKFL 162          
BLAST of KH domain-containing protein vs. Ensembl Medaka
Match: khdrbs1a (KH domain-containing, RNA-binding, signal transduction-associated protein 1 [Source:NCBI gene;Acc:101175524])

HSP 1 Score: 78.5666 bits (192), Expect = 5.221e-16
Identity = 54/173 (31.21%), Postives = 90/173 (52.02%), Query Frame = 3
Query:  144 QIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIAD-------FIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNM---------PVFDH-TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVDPNNQNA 611
            +++A ++ L+   T    +L    A+EI R++ G          ++DL   K +K++ ++ IP  QYP  NFVGKLLGP  ST+K +QE+T  K+++ G  + + KN            + H   +LH+ I+      EAY +++HA+ EV+K L        P +DP+  N 
Sbjct:    9 ELLAEKDSLDSSFTHAMKLL----AAEIERIQKGETKKEPEKETYLDLFATKSLKLKERVLIPIKQYPRVNFVGKLLGPQGSTIKRLQEETGAKISVLGKGSMRDKNKEEELRKGGEAKYAHLAMELHVFIEVTAPIPEAYMRMAHAMDEVKKFLIPEPGEHEPYMDPHFLNG 177          
BLAST of KH domain-containing protein vs. Planmine SMEST
Match: SMESG000068335.1 (SMESG000068335.1)

HSP 1 Score: 479.559 bits (1233), Expect = 6.891e-173
Identity = 249/250 (99.60%), Postives = 249/250 (99.60%), Query Frame = 3
Query:   93 MADAPELKISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVDPNNQNAVLVSTEYNMAPRVYTTPXXXXXXXXXXXXXALRVSPRFVPPPAPTEWLNPEEANGGQELISGGPIHNEKNLNRITPY 842
            MADAPELKISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVDPNNQNAV VSTEYNMAPRVYTTPIRGIQRGGNRRGNALRVSPRFVPPPAPTEWLNPEEANGGQELISGGPIHNEKNLNRITPY
Sbjct:    1 MADAPELKISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGIENPKQKNMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPFSYPTVDPNNQNAVQVSTEYNMAPRVYTTPIRGIQRGGNRRGNALRVSPRFVPPPAPTEWLNPEEANGGQELISGGPIHNEKNLNRITPY 250          
BLAST of KH domain-containing protein vs. Planmine SMEST
Match: SMESG000057199.1 (SMESG000057199.1)

HSP 1 Score: 182.57 bits (462), Expect = 4.930e-56
Identity = 99/181 (54.70%), Postives = 123/181 (67.96%), Query Frame = 3
Query:  129 FIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---ENPKQKNMPVFDH-----TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPF----SYPTVDPNNQNAVLVSTEYN 635
            ++D  QI  ++ EL+ I     P L+SLG +E+HRLRGGIAD +DL  NKKVK+R K+ IP DQYP+ NFVGKLLGPGASTLK IQ QTNTKMTI G+   +N K++     D       +KLHLQIDSLGTPAEAYY+LSHA+ EVQ C+   LP     SY  V  N  N ++ S+ Y+
Sbjct:   10 YVDQSQIDILEQELKEIDD-KYPYLASLGEAELHRLRGGIADSVDLFSNKKVKIRAKVHIPVDQYPSVNFVGKLLGPGASTLKGIQGQTNTKMTILGVGVLKNKKEEFQNKIDKKNISTKQKLHLQIDSLGTPAEAYYRLSHALVEVQNCMYHELPSGLVQSYHPVAYNWNNYMINSSTYD 189          
BLAST of KH domain-containing protein vs. Planmine SMEST
Match: SMESG000057199.1 (SMESG000057199.1)

HSP 1 Score: 182.57 bits (462), Expect = 5.361e-56
Identity = 99/181 (54.70%), Postives = 123/181 (67.96%), Query Frame = 3
Query:  129 FIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---ENPKQKNMPVFDH-----TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPF----SYPTVDPNNQNAVLVSTEYN 635
            ++D  QI  ++ EL+ I     P L+SLG +E+HRLRGGIAD +DL  NKKVK+R K+ IP DQYP+ NFVGKLLGPGASTLK IQ QTNTKMTI G+   +N K++     D       +KLHLQIDSLGTPAEAYY+LSHA+ EVQ C+   LP     SY  V  N  N ++ S+ Y+
Sbjct:   10 YVDQSQIDILEQELKEIDD-KYPYLASLGEAELHRLRGGIADSVDLFSNKKVKIRAKVHIPVDQYPSVNFVGKLLGPGASTLKGIQGQTNTKMTILGVGVLKNKKEEFQNKIDKKNISTKQKLHLQIDSLGTPAEAYYRLSHALVEVQNCMYHELPSGLVQSYHPVAYNWNNYMINSSTYD 189          
BLAST of KH domain-containing protein vs. Planmine SMEST
Match: SMESG000057199.1 (SMESG000057199.1)

HSP 1 Score: 174.481 bits (441), Expect = 5.603e-53
Identity = 98/181 (54.14%), Postives = 121/181 (66.85%), Query Frame = 3
Query:  129 FIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---ENPKQKNMPVFDH-----TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPF----SYPTVDPNNQNAVLVSTEYN 635
            ++D  QI  ++ EL+ I     P L+SL   E+HRLRGGIAD +DL  NKKVK+R K+ IP DQYP+ NFVGKLLGPGASTLK IQ QTNTKMTI G+   +N K++     D       +KLHLQIDSLGTPAEAYY+LSHA+ EVQ C+   LP     SY  V  N  N ++ S+ Y+
Sbjct:   10 YVDQSQIDILEQELKEIDD-KYPYLASL---ELHRLRGGIADSVDLFSNKKVKIRAKVHIPVDQYPSVNFVGKLLGPGASTLKGIQGQTNTKMTILGVGVLKNKKEEFQNKIDKKNISTKQKLHLQIDSLGTPAEAYYRLSHALVEVQNCMYHELPSGLVQSYHPVAYNWNNYMINSSTYD 186          
BLAST of KH domain-containing protein vs. Planmine SMEST
Match: SMESG000057199.1 (SMESG000057199.1)

HSP 1 Score: 174.866 bits (442), Expect = 6.272e-53
Identity = 98/181 (54.14%), Postives = 121/181 (66.85%), Query Frame = 3
Query:  129 FIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRGGIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQTNTKMTIRGI---ENPKQKNMPVFDH-----TEKLHLQIDSLGTPAEAYYKLSHAIAEVQKCLTFNLPF----SYPTVDPNNQNAVLVSTEYN 635
            ++D  QI  ++ EL+ I     P L+SL   E+HRLRGGIAD +DL  NKKVK+R K+ IP DQYP+ NFVGKLLGPGASTLK IQ QTNTKMTI G+   +N K++     D       +KLHLQIDSLGTPAEAYY+LSHA+ EVQ C+   LP     SY  V  N  N ++ S+ Y+
Sbjct:   10 YVDQSQIDILEQELKEIDD-KYPYLASL---ELHRLRGGIADSVDLFSNKKVKIRAKVHIPVDQYPSVNFVGKLLGPGASTLKGIQGQTNTKMTILGVGVLKNKKEEFQNKIDKKNISTKQKLHLQIDSLGTPAEAYYRLSHALVEVQNCMYHELPSGLVQSYHPVAYNWNNYMINSSTYD 186          
The following BLAST results are available for this feature:
BLAST of KH domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KHDRBS31.394e-1939.64KH RNA binding domain containing, signal transduct... [more]
KHDRBS27.079e-1936.30KH RNA binding domain containing, signal transduct... [more]
KHDRBS39.706e-1939.64KH RNA binding domain containing, signal transduct... [more]
KHDRBS33.099e-1839.64KH RNA binding domain containing, signal transduct... [more]
KHDRBS14.731e-1633.08KH RNA binding domain containing, signal transduct... [more]
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BLAST of KH domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
asd-26.633e-1034.74RNA-binding protein asd-2 [Source:UniProtKB/Swiss... [more]
asd-21.141e-934.74RNA-binding protein asd-2 [Source:UniProtKB/Swiss... [more]
asd-21.194e-934.74RNA-binding protein asd-2 [Source:UniProtKB/Swiss... [more]
asd-21.352e-934.74RNA-binding protein asd-2 [Source:UniProtKB/Swiss... [more]
Y69A2AR.323.453e-829.52pep chromosome:WBcel235:IV:2610408:2612430:1 gene:... [more]
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BLAST of KH domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
qkr58E-31.342e-1633.91gene:FBgn0022984 transcript:FBtr0339538[more]
qkr58E-31.342e-1633.91gene:FBgn0022984 transcript:FBtr0307214[more]
qkr58E-31.342e-1633.91gene:FBgn0022984 transcript:FBtr0290254[more]
CG39272.374e-1532.17gene:FBgn0034739 transcript:FBtr0071853[more]
CG40212.821e-1532.11gene:FBgn0034659 transcript:FBtr0071716[more]
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BLAST of KH domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
khdrbs26.025e-1932.92KH domain containing, RNA binding, signal transduc... [more]
khdrbs1a3.452e-1632.03KH domain containing, RNA binding, signal transduc... [more]
khdrbs1a3.745e-1632.03KH domain containing, RNA binding, signal transduc... [more]
khdrbs1a4.091e-1632.03KH domain containing, RNA binding, signal transduc... [more]
khdrbs1a4.091e-1632.03KH domain containing, RNA binding, signal transduc... [more]
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BLAST of KH domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
khdrbs28.476e-1837.39KH domain containing, RNA binding, signal transduc... [more]
KHDRBS31.653e-1737.30KH domain containing, RNA binding, signal transduc... [more]
pdc3.153e-1735.62phosducin [Source:Xenbase;Acc:XB-GENE-983832][more]
pdc6.164e-1735.51phosducin [Source:Xenbase;Acc:XB-GENE-983832][more]
spsb31.711e-831.11splA/ryanodine receptor domain and SOCS box contai... [more]
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BLAST of KH domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Khdrbs25.962e-1936.30KH domain containing, RNA binding, signal transduc... [more]
Khdrbs32.087e-1839.64KH domain containing, RNA binding, signal transduc... [more]
Khdrbs32.087e-1839.64KH domain containing, RNA binding, signal transduc... [more]
Khdrbs32.087e-1839.64KH domain containing, RNA binding, signal transduc... [more]
Khdrbs33.006e-1639.22KH domain containing, RNA binding, signal transduc... [more]
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BLAST of KH domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P13230|GRP33_ARTSA5.944e-1937.01Glycine-rich protein GRP33 OS=Artemia salina OX=85... [more]
sp|Q5VWX1|KHDR2_HUMAN3.400e-1836.30KH domain-containing, RNA-binding, signal transduc... [more]
sp|Q920F3|KHDR2_RAT3.711e-1836.30KH domain-containing, RNA-binding, signal transduc... [more]
sp|Q9WU01|KHDR2_MOUSE4.170e-1836.30KH domain-containing, RNA-binding, signal transduc... [more]
sp|Q08BJ2|KHDR2_DANRE4.548e-1832.92KH domain-containing, RNA-binding, signal transduc... [more]
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BLAST of KH domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A4V6L07.445e-12677.37Sam68-like mammalian protein 1 (Fragment) OS=Duges... [more]
A0A504Y6P73.339e-3848.13KH domain containing RNA binding signal OS=Fasciol... [more]
A0A2H1C8T83.339e-3848.13KH domain containing RNA binding signal OS=Fasciol... [more]
A0A448X8Z07.606e-3752.59KH domain-containing protein (Fragment) OS=Protopo... [more]
C1L8187.669e-3751.02KH domain-containing, RNA-binding, signal transduc... [more]
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BLAST of KH domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
khdrbs21.592e-1836.97KH domain containing, RNA binding, signal transduc... [more]
khdrbs22.360e-1836.97KH domain containing, RNA binding, signal transduc... [more]
khdrbs22.376e-1836.97KH domain containing, RNA binding, signal transduc... [more]
khdrbs22.607e-1836.97KH domain containing, RNA binding, signal transduc... [more]
khdrbs22.997e-1836.97KH domain containing, RNA binding, signal transduc... [more]
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BLAST of KH domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
khdrbs22.534e-2041.44KH domain containing, RNA binding, signal transduc... [more]
KHDRBS35.405e-1836.61KH RNA binding domain containing, signal transduct... [more]
khdrbs1a1.208e-1336.64KH domain containing, RNA binding, signal transduc... [more]
sf19.136e-744.44splicing factor 1 [Source:ZFIN;Acc:ZDB-GENE-030131... [more]
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BLAST of KH domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
MSL56.231e-952.08Component of commitment complex; which defines fir... [more]
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BLAST of KH domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO441274.539e-1338.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO302975.103e-1031.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO454841.187e-736.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO286411.476e-635.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of KH domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
KHDRBS32.457e-1942.34KH RNA binding domain containing, signal transduct... [more]
KHDRBS32.779e-1942.34KH RNA binding domain containing, signal transduct... [more]
khdrbs21.367e-1833.54KH RNA binding domain containing, signal transduct... [more]
khdrbs21.464e-1834.29KH RNA binding domain containing, signal transduct... [more]
khdrbs1a5.221e-1631.21KH domain-containing, RNA-binding, signal transduc... [more]
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BLAST of KH domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000068335.16.891e-17399.60SMESG000068335.1[more]
SMESG000057199.14.930e-5654.70SMESG000057199.1[more]
SMESG000057199.15.361e-5654.70SMESG000057199.1[more]
SMESG000057199.15.603e-5354.14SMESG000057199.1[more]
SMESG000057199.16.272e-5354.14SMESG000057199.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003741 ID=SMED30003741|Name=KH domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=971bp
AATATTTACCAATTTTAAAAATTACTAATTGAGAATTTCTTTTGTCTTTG
TTTTAAATTACATATCGTTATTTAATATTTAAATATTTTAAAATGGCAGA
TGCTCCAGAATTAAAAATTTCAAAACCATTTATTGATGATCAACAAATAA
TTGCAATTCAGAATGAATTGGAAATTATTAGAACCCTAAATTGTCCTATT
TTATCTAGTCTTGGAGCTTCTGAAATCCACAGGTTGAGAGGAGGAATAGC
TGATTTTATAGATTTAATTTTAAATAAAAAAGTTAAAGTAAGAACTAAAA
TTGATATACCAGCGGATCAGTATCCAACGACTAATTTTGTTGGGAAATTA
CTTGGACCTGGTGCAAGTACTTTGAAAAGTATTCAAGAACAAACCAATAC
CAAAATGACAATTCGTGGAATTGAAAATCCAAAGCAAAAGAATATGCCTG
TTTTCGATCACACTGAAAAATTACATTTACAGATAGATAGTTTAGGAACA
CCAGCTGAGGCTTACTATAAATTATCTCACGCAATTGCTGAAGTACAAAA
GTGCTTAACATTTAACCTTCCGTTCAGTTATCCTACTGTGGACCCTAATA
ATCAAAATGCAGTTCTGGTAAGTACAGAATATAATATGGCTCCACGTGTG
TACACAACACCAATAAGAGGTATCCAACGAGGTGGAAATAGAAGAGGAAA
CGCTCTACGAGTTAGTCCACGATTTGTCCCTCCTCCAGCTCCAACCGAAT
GGTTGAATCCTGAAGAAGCCAATGGCGGGCAAGAATTGATATCAGGAGGT
CCTATTCACAATGAAAAGAATTTGAATCGTATCACTCCATACTAAGGCAT
CAATAACAGTGAAAATTAATGTATTATAATATTAAACGTTTAATTGCAAA
TTGGAGTTCTGTTTTTATTAAATATTTTGGATATTATTTAACAATCAAAA
TTTTTGTTTGGCGTAAACCTG
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protein sequence of SMED30003741-orf-1

>SMED30003741-orf-1 ID=SMED30003741-orf-1|Name=SMED30003741-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=251bp
MADAPELKISKPFIDDQQIIAIQNELEIIRTLNCPILSSLGASEIHRLRG
GIADFIDLILNKKVKVRTKIDIPADQYPTTNFVGKLLGPGASTLKSIQEQ
TNTKMTIRGIENPKQKNMPVFDHTEKLHLQIDSLGTPAEAYYKLSHAIAE
VQKCLTFNLPFSYPTVDPNNQNAVLVSTEYNMAPRVYTTPIRGIQRGGNR
RGNALRVSPRFVPPPAPTEWLNPEEANGGQELISGGPIHNEKNLNRITPY
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036612KH_dom_type_1_sf
IPR004087KH_dom
IPR004088KH_dom_type_1
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0003723RNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004087K Homology domainSMARTSM00322kh_6coord: 64..153
e-value: 4.1E-6
score: 36.3
IPR036612K Homology domain, type 1 superfamilyGENE3DG3DSA:3.30.1370.10coord: 16..162
e-value: 1.5E-24
score: 89.2
IPR036612K Homology domain, type 1 superfamilySUPERFAMILYSSF54791Eukaryotic type KH-domain (KH-domain type I)coord: 67..156
IPR004088K Homology domain, type 1PFAMPF00013KH_1coord: 69..114
e-value: 9.9E-7
score: 28.5
NoneNo IPR availablePANTHERPTHR11208RNA-BINDING PROTEIN RELATEDcoord: 49..176
NoneNo IPR availablePANTHERPTHR11208:SF43GH05812P-RELATEDcoord: 49..176