Dynein light chain

Overview
NameDynein light chain
Smed IDSMED30003729
Length (bp)1397
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Dynein light chain (SMED30003729) t-SNE clustered cells

Violin plots show distribution of expression levels for Dynein light chain (SMED30003729) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dynein light chain (SMED30003729) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dynein light chain (SMED30003729) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30003729

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003729SMESG000048100.1 Contig41096newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003729SMESG000048100.1 Contig41096uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30003729SMESG000048100.1 dd_Smed_v6_5884_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30003729SMESG000048100.1 dd_Smed_v4_5884_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Dynein light chain vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 46.595 bits (109), Expect = 1.545e-6
Identity = 21/82 (25.61%), Postives = 47/82 (57.32%), Query Frame = 1 
Query:  955 ISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            + ++ DM+D ++   ++ +  +  ++N   ++A  IK+  D  +N +WH + G++ +G  VTHE   FI+    +  IL+++
Sbjct:    7 VIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRN-FGSYVTHETKHFIYFYLGQVAILLFK 87
[Close]
BLAST of Dynein light chain vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 46.595 bits (109), Expect = 1.545e-6
Identity = 21/82 (25.61%), Postives = 47/82 (57.32%), Query Frame = 1 
Query:  955 ISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            + ++ DM+D ++   ++ +  +  ++N   ++A  IK+  D  +N +WH + G++ +G  VTHE   FI+    +  IL+++
Sbjct:    7 VIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRN-FGSYVTHETKHFIYFYLGQVAILLFK 87
[Close]
BLAST of Dynein light chain vs. Ensembl Celegans
Match: dlc-1 (Dynein light chain 1, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q22799])

HSP 1 Score: 46.595 bits (109), Expect = 1.545e-6
Identity = 21/82 (25.61%), Postives = 47/82 (57.32%), Query Frame = 1 
Query:  955 ISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            + ++ DM+D ++   ++ +  +  ++N   ++A  IK+  D  +N +WH + G++ +G  VTHE   FI+    +  IL+++
Sbjct:    7 VIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRN-FGSYVTHETKHFIYFYLGQVAILLFK 87
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|Q759T0|DYL1_ASHGO (Dynein light chain 1, cytoplasmic OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=DYN2 PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 7.691e-10
Identity = 31/81 (38.27%), Postives = 49/81 (60.49%), Query Frame = 1 
Query:  970 DMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT 1212
            D+TD LR+ +  +S+ +  +F    E+A  IK+ LD    ++WHV+ GK+ +G  VTHE+G FI+      G L + +FKT
Sbjct:   11 DITDELRDEIFGISVQAVEQFQLEREVAAYIKKELDSKHGQTWHVIVGKN-FGSYVTHEKGHFIYFYI---GPLAFLVFKT 87
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|Q6CWX4|DYL1_KLULA (Dynein light chain 1, cytoplasmic OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=DYN2 PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.198e-8
Identity = 30/81 (37.04%), Postives = 48/81 (59.26%), Query Frame = 1 
Query:  970 DMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT 1212
            D+TD LR+ +  LS ++   +    E+A  IK+ LD +   +WHV+ GK+ +G  VTHE+G F++      G L + +FKT
Sbjct:   10 DITDELRDEIFELSSNATANYKLEREIAAYIKKQLDVSQGETWHVIVGKN-FGSYVTHEKGYFVYFYI---GPLAFLVFKT 86
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|Q02647|DYL1_YEAST (Dynein light chain 1, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DYN2 PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.308e-8
Identity = 29/86 (33.72%), Postives = 51/86 (59.30%), Query Frame = 1 
Query:  955 ISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT 1212
            I +  D+TD L+  +L +S D+  ++    ++A  +K+ LD  +  +WHV+ GK+ +G  VTHE+G F++      G L + +FKT
Sbjct:   10 IVKASDITDKLKEDILTISKDALDKYQLERDIAGTVKKQLDVKYGNTWHVIVGKN-FGSYVTHEKGHFVYFYI---GPLAFLVFKT 91
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|Q6FUJ0|DYL1_CANGA (Dynein light chain 1, cytoplasmic OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=DYN2 PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 9.528e-8
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1 
Query:  952 VISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT 1212
            VI +  DM D ++  V  ++ ++ R      E+A  IK+ +D  +  +WHV+ G+ ++G  VTHE+G+F++      G L   +FKT
Sbjct:    4 VIVKASDMGDEMQQEVFRIAEEAMREHTLEREIASVIKKEMDSRYGHTWHVIVGR-SFGSYVTHEKGKFVYFYV---GPLALLVFKT 86
[Close]
BLAST of Dynein light chain vs. UniProt/SwissProt
Match: sp|P14202|TEGU_SCHMA (Tegument antigen OS=Schistosoma mansoni OX=6183 GN=A12 PE=2 SV=1)

HSP 1 Score: 50.0618 bits (118), Expect = 9.038e-6
Identity = 25/62 (40.32%), Postives = 34/62 (54.84%), Query Frame = 1 
Query:  262 EFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGL 447
            EFI+AF EID D +  I + EL +Y +        ++ W+  FD D    IS+EEFC   GL
Sbjct:   12 EFIRAFLEIDADSNEMIDKQELIKYCQKYRLDMKLIDPWIARFDTDKDNKISIEEFCRGFGL 73
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A267EIW9 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005207g1 PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 7.832e-21
Identity = 44/74 (59.46%), Postives = 58/74 (78.38%), Query Frame = 1 
Query:  259 MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQ 480
            M+F+ AFE+ID D SGEIT  ELE Y +  NY+  FVEKW+R+FD DNSGTI++EEFC TLGL+P ++  E++Q
Sbjct:    1 MDFLHAFEDIDSDFSGEITTEELEAYCRRHNYEDRFVEKWLRLFDHDNSGTITIEEFCDTLGLVPKQEYMEKVQ 74

HSP 2 Score: 64.6994 bits (156), Expect = 1.966e-8
Identity = 27/87 (31.03%), Postives = 50/87 (57.47%), Query Frame = 1 
Query:  940 DEIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            D + +I+EDP+++DS +  ++ ++  +  +F    ++A+ +K  LD  F R+WHV+ G+  YG   THE G   H       +L+W+
Sbjct:   87 DGVKIITEDPELSDSTKEEIVRIARQAQEQFVNERDIARHMKARLDDMFGRAWHVIVGRYQYGSFCTHETGSLFHFYVLRNAVLVWK 173
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A1I8IZM2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 2.963e-20
Identity = 44/74 (59.46%), Postives = 58/74 (78.38%), Query Frame = 1 
Query:  259 MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQ 480
            M+F+ AFE+ID D SGEIT  ELE Y +  NY+  FVEKW+R+FD DNSGTI++EEFC TLGL+P ++  E++Q
Sbjct:    1 MDFLHAFEDIDSDFSGEITTEELEAYCRRHNYEDRFVEKWLRLFDHDNSGTITIEEFCDTLGLVPKQEYMEKVQ 74

HSP 2 Score: 60.077 bits (144), Expect = 1.560e-6
Identity = 25/75 (33.33%), Postives = 45/75 (60.00%), Query Frame = 1 
Query:  940 DEIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIH 1164
            D + +I+EDP+++DS +  ++ ++  +  +F    ++A+ +K  LD  F R+WHV+ G+  YG   THE G   H
Sbjct:   87 DGVKIITEDPELSDSTKEEIVRIARQAQEQFVNERDIARHMKARLDDMFGRAWHVIVGRYQYGSFCTHETGSLFH 161
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A1I8I7N8 (Dynein light chain OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001380g1 PE=3 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 3.803e-20
Identity = 43/74 (58.11%), Postives = 57/74 (77.03%), Query Frame = 1 
Query:  259 MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQ 480
            M+F+ A+E ID D SGEIT  ELE Y +  NY+  FVEKW+R+FD DNSGTI++EEFC TLGL+P ++  E++Q
Sbjct:    1 MDFLTAYEAIDTDFSGEITSEELEAYCRKHNYEDKFVEKWLRLFDHDNSGTITIEEFCETLGLVPKQEYMEKVQ 74

HSP 2 Score: 75.485 bits (184), Expect = 2.971e-12
Identity = 33/87 (37.93%), Postives = 52/87 (59.77%), Query Frame = 1 
Query:  940 DEIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            D + +I+EDPDMTD L+  ++ L+  +  +F    ++A+ +K  LD  F+R+WHV+ G+  YG   THE GR  H       IL+W+
Sbjct:   85 DGVKIITEDPDMTDELKEEIVQLARTAQEQFENERDIARYMKSELDAKFDRAWHVIVGRFQYGSFCTHEVGRLFHFYVVRNAILVWK 171
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A1I8IZR3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 3.710e-19
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 1 
Query:  253 LHMEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQ 480
            + M+F+ AFE+ID D SGEIT  ELE Y +  NY+  FVEKW+R+FD DNSGTI++EEFC TLGL+P ++  E++Q
Sbjct:  281 ISMDFLHAFEDIDSDFSGEITTEELEAYCRRHNYEDRFVEKWLRLFDHDNSGTITIEEFCDTLGLVPKQEYMEKVQ 356

HSP 2 Score: 64.3142 bits (155), Expect = 2.802e-7
Identity = 27/87 (31.03%), Postives = 50/87 (57.47%), Query Frame = 1 
Query:  940 DEIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            D + +I+EDP+++DS +  ++ ++  +  +F    ++A+ +K  LD  F R+WHV+ G+  YG   THE G   H       +L+W+
Sbjct:  369 DGVKIITEDPELSDSTKEEIVRIARQAQEQFVNERDIARHMKARLDDMFGRAWHVIVGRYQYGSFCTHETGSLFHFYVLRNAVLVWK 455
[Close]
BLAST of Dynein light chain vs. TrEMBL
Match: A0A267GM98 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016025g1 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.033e-18
Identity = 41/77 (53.25%), Postives = 56/77 (72.73%), Query Frame = 1 
Query:  262 EFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQRRNS 492
            +F+ AFE ID D SGEITR ELE Y + +N+   FV+KW+ +FD DNSGTIS++E+C TLGL P  +  E++Q + S
Sbjct:    3 DFLTAFESIDTDFSGEITREELEAYCRKQNFDEKFVQKWLNLFDADNSGTISIDEYCDTLGLRPNTEYMEKVQVQRS 79
[Close]
BLAST of Dynein light chain vs. Ensembl Yeast
Match: DYN2 (Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments [Source:SGD;Acc:S000002832])

HSP 1 Score: 55.4546 bits (132), Expect = 1.884e-10
Identity = 29/86 (33.72%), Postives = 51/86 (59.30%), Query Frame = 1 
Query:  955 ISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT 1212
            I +  D+TD L+  +L +S D+  ++    ++A  +K+ LD  +  +WHV+ GK+ +G  VTHE+G F++      G L + +FKT
Sbjct:   10 IVKASDITDKLKEDILTISKDALDKYQLERDIAGTVKKQLDVKYGNTWHVIVGKN-FGSYVTHEKGHFVYFYI---GPLAFLVFKT 91
[Close]
BLAST of Dynein light chain vs. Ensembl Nematostella
Match: EDO44582 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVI5])

HSP 1 Score: 44.2838 bits (103), Expect = 4.410e-6
Identity = 21/82 (25.61%), Postives = 45/82 (54.88%), Query Frame = 1 
Query:  955 ISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            + ++ DM + ++   +  +  +  +FN   ++A  IK+  D  +N +WH + G++ +G  VTHE   FI+    +  IL+++
Sbjct:    7 VIKNADMAEDMQTDAIECATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRN-FGSYVTHETKHFIYFYLGQVAILLFK 87
[Close]
BLAST of Dynein light chain vs. Ensembl Medaka
Match: DYNLL2 (dynein light chain 2, cytoplasmic [Source:NCBI gene;Acc:101174042])

HSP 1 Score: 47.3654 bits (111), Expect = 1.128e-6
Identity = 24/88 (27.27%), Postives = 52/88 (59.09%), Query Frame = 1 
Query:  937 SDEIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            SD+  VI ++ DM+D ++   ++ ++ +  ++N   ++A  +K+  D  +N +WH + G++ +G  VTHE   FI+    +  IL+++
Sbjct:    2 SDKKAVI-KNADMSDEMQQDAVDCAMQAMEKYNIEKDIAAYVKKEFDKKYNPTWHCIVGRN-FGSYVTHETKHFIYFYLGQVAILLFK 87
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000048100.1 (SMESG000048100.1)

HSP 1 Score: 585.489 bits (1508), Expect = 0.000e+0
Identity = 313/318 (98.43%), Postives = 317/318 (99.69%), Query Frame = 1 
Query:  259 MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQRRNSQKQLELEAKSKKKSNEEDTQTXXXXXXXXKVISKDITVIIENPNYTTTQSFNGLEKNNSIEIESRKESNLIENDKSKEIDKQDSNQNSRSHEQMISLSTSLDRTKTDGESKLSPVKCLNEINQKXXXXXXXXXXXXXXXXXFKRIIIPSDEIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT 1212
            MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQRRNSQKQLELE KSKKKSNEEDTQTSNFSSSNSKVISK+ITVIIENPNYTTTQSFNGLEKNNSIEIESRKESNLIENDKSKEIDKQDSNQNSRSHEQ+ISLSTSLDRTKTDGESKLSPVKCLNEINQKETETRE+IEEYERMITRFKRIIIPSDEIYVISEDPDMT+SLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT
Sbjct:    1 MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQRRNSQKQLELEGKSKKKSNEEDTQTSNFSSSNSKVISKNITVIIENPNYTTTQSFNGLEKNNSIEIESRKESNLIENDKSKEIDKQDSNQNSRSHEQIISLSTSLDRTKTDGESKLSPVKCLNEINQKETETRESIEEYERMITRFKRIIIPSDEIYVISEDPDMTESLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQIFKT 318
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000042102.1 (SMESG000042102.1)

HSP 1 Score: 132.494 bits (332), Expect = 2.989e-36
Identity = 61/82 (74.39%), Postives = 71/82 (86.59%), Query Frame = 1 
Query:  259 MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQRRNSQKQL 504
            MEFIKAFEEID D SGEITRGEL  YM+ KNYKPAFVEKW+ IFD DNSGTI+LEEFC+TLGLIP K  ++RI++R S+K+L
Sbjct:    1 MEFIKAFEEIDSDNSGEITRGELIRYMRLKNYKPAFVEKWLNIFDADNSGTITLEEFCNTLGLIPNKNYQKRIEKRKSEKEL 82

HSP 2 Score: 87.4261 bits (215), Expect = 7.323e-20
Identity = 36/95 (37.89%), Postives = 61/95 (64.21%), Query Frame = 1 
Query:  919 KRIIIPSDEIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQI 1203
            K ++IP  ++YVI EDP      ++F + +  ++    N+ + +AK +K +LD  F RSW+VV   DAYG  ++HE G FIH++ R++ IL+W++
Sbjct:   86 KSLLIPLYDLYVIDEDPLNDPKFKDFCMKICQEATNNENDFISIAKYLKSVLDVKFGRSWNVVVDIDAYGSFLSHEPGAFIHIKVRKFAILLWKV 180
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000008592.1 (SMESG000008592.1)

HSP 1 Score: 90.8929 bits (224), Expect = 5.006e-21
Identity = 46/97 (47.42%), Postives = 65/97 (67.01%), Query Frame = 1 
Query:  259 MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQRRN--SQKQLELEAKSKKKSNEE 543
            M+ IKAFEEID D SGEIT+ EL  YM  K+Y+  FV KWM IFD +N+G I+LEEFC  LGL P +   +++Q +   S+  +++     +++NEE
Sbjct:    1 MDLIKAFEEIDTDYSGEITKDELINYMIKKDYEMGFVNKWMEIFDPNNTGRITLEEFCDALGLQPNEDYLQQVQTKQEESKPMIQICPPFNEETNEE 97

HSP 2 Score: 71.633 bits (174), Expect = 2.604e-14
Identity = 28/86 (32.56%), Postives = 52/86 (60.47%), Query Frame = 1 
Query:  943 EIYVISEDPDMTDSLRNFVLNLSLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRFIHVRAREWGILMWQ 1200
            ++ +I EDPD T   R F +++S  +++      ++AK IK+ LD  +N+ WHVV  +  +G  ++H+ G+F+H +   +  L+WQ
Sbjct:  100 DVEIIREDPDTTAEFRQFAIDVSHQASKDHKLEKDIAKSIKKKLDEEYNKIWHVVVARSTFGSFISHKSGKFMHYKIENFCYLIWQ 185
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000045029.1 (SMESG000045029.1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.430e-19
Identity = 41/93 (44.09%), Postives = 61/93 (65.59%), Query Frame = 1 
Query:  265 FIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGLIPGKKQKERIQRRNSQKQLELEAKSKKKSNEE 543
            FI+AF  ID D SGEI+  EL +YM   +Y+  F++KW++IFDIDNSGTI+LEE+C  LG+   + ++  +QRR S   +    K  + + +E
Sbjct:    4 FIEAFFAIDTDSSGEISTDELSDYMNKNHYRSEFIKKWLKIFDIDNSGTITLEEYCKVLGI---EYKRASLQRRMSMYSIPENVKILQSTMDE 93
[Close]
BLAST of Dynein light chain vs. Planmine SMEST
Match: SMESG000006950.1 (SMESG000006950.1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.302e-18
Identity = 38/61 (62.30%), Postives = 45/61 (73.77%), Query Frame = 1 
Query:  265 FIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSGTISLEEFCSTLGL 447
            FIKA+ +IDKD SG IT  EL  YM   NY+  FV KW+R+FD DN+G I+LEEFC TLGL
Sbjct:    5 FIKAYCQIDKDGSGTITADELRAYMTENNYQEDFVTKWLRLFDRDNTGRITLEEFCETLGL 65
The following BLAST results are available for this feature:
BLAST of Dynein light chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Dynein light chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
dlc-11.545e-625.61Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-11.545e-625.61Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
dlc-11.545e-625.61Dynein light chain 1, cytoplasmic [Source:UniProt... [more]
back to top
BLAST of Dynein light chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Dynein light chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Dynein light chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Dynein light chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Dynein light chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q759T0|DYL1_ASHGO7.691e-1038.27Dynein light chain 1, cytoplasmic OS=Ashbya gossyp... [more]
sp|Q6CWX4|DYL1_KLULA1.198e-837.04Dynein light chain 1, cytoplasmic OS=Kluyveromyces... [more]
sp|Q02647|DYL1_YEAST1.308e-833.72Dynein light chain 1, cytoplasmic OS=Saccharomyces... [more]
sp|Q6FUJ0|DYL1_CANGA9.528e-832.18Dynein light chain 1, cytoplasmic OS=Candida glabr... [more]
sp|P14202|TEGU_SCHMA9.038e-640.32Tegument antigen OS=Schistosoma mansoni OX=6183 GN... [more]
back to top
BLAST of Dynein light chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267EIW97.832e-2159.46Dynein light chain OS=Macrostomum lignano OX=28230... [more]
A0A1I8IZM22.963e-2059.46Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I7N83.803e-2058.11Dynein light chain OS=Macrostomum lignano OX=28230... [more]
A0A1I8IZR33.710e-1957.89Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GM981.033e-1853.25Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
back to top
BLAST of Dynein light chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Dynein light chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Dynein light chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
DYN21.884e-1033.72Cytoplasmic light chain dynein, microtubule motor ... [more]
back to top
BLAST of Dynein light chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO445824.410e-625.61Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Dynein light chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
DYNLL21.128e-627.27dynein light chain 2, cytoplasmic [Source:NCBI gen... [more]
back to top
BLAST of Dynein light chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000048100.10.000e+098.43SMESG000048100.1[more]
SMESG000042102.12.989e-3674.39SMESG000042102.1[more]
SMESG000008592.15.006e-2147.42SMESG000008592.1[more]
SMESG000045029.11.430e-1944.09SMESG000045029.1[more]
SMESG000006950.11.302e-1862.30SMESG000006950.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003729 ID=SMED30003729|Name=Dynein light chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1397bp
CCTAGAGATTGCTAAAATCTAAACTTATTAACAAGGTGATGGATTCATAG
ATCAGAAAACTACATAAGGTTATCCGAGATATATTTGTGTGTGTGTGCAC
GCGCGCATTCTCAAACAGTGCTTCATGCACACATCGATGCTTCAGTAGCA
TCACAACCCGATGCCAATTCTGTGCGTTTGTCTACAAAACTTATCAAAAG
TCACTGCTCATTCTAGTTGTAGTATTTTTAGAATTTTCAATTTGTGATAT
TTCTTCATATGGAATTTATCAAAGCTTTTGAAGAAATTGACAAAGATGAA
TCTGGAGAAATAACTCGCGGTGAACTCGAAGAATATATGAAAGCCAAAAA
CTATAAGCCAGCATTTGTTGAGAAATGGATGAGAATATTCGACATTGATA
ATTCGGGCACGATTTCATTGGAAGAGTTCTGCAGTACCCTTGGCTTAATA
CCCGGTAAGAAGCAAAAAGAAAGAATACAGCGTCGAAATTCTCAAAAGCA
GTTAGAATTAGAAGCTAAAAGTAAAAAGAAATCAAATGAAGAAGACACTC
AAACATCAAACTTCAGTTCCAGCAATTCGAAAGTTATTAGCAAAGACATA
ACGGTAATCATTGAAAATCCCAACTATACTACAACTCAAAGCTTTAATGG
ACTCGAAAAGAATAATTCCATCGAAATTGAGTCTAGAAAAGAGAGCAATT
TAATAGAAAATGATAAATCGAAGGAAATAGATAAACAGGATTCGAATCAA
AATTCTAGATCACATGAGCAAATGATTTCGTTGTCGACAAGTTTAGACAG
AACTAAAACCGACGGGGAATCCAAGCTTTCACCAGTTAAATGTCTCAATG
AAATTAATCAAAAAGAAACCGAAACTAGAGAAACTATTGAGGAATACGAA
AGAATGATAACGAGATTCAAAAGGATTATTATTCCGAGTGATGAAATATA
CGTTATATCCGAAGATCCTGATATGACTGACTCACTGAGGAATTTTGTAC
TAAACCTGAGCCTAGATTCCGCGAGAAGATTCAATGAGCCACTAGAAATG
GCAAAGTGTATAAAAAGAATTCTTGATTTTACTTTCAATCGGTCATGGCA
TGTTGTGACTGGGAAAGATGCATATGGGTGTTTAGTAACTCACGAAGAGG
GTAGATTTATTCATGTGCGAGCCAGAGAGTGGGGTATACTTATGTGGCAG
ATTTTCAAGACTTAATTCGAGGAAATTCACTTGTTTTTTTGTGATTGTTA
ATTTTCTCTTGATATACATGATGACTGTGGCTGGGCTTCTTTTGCAATTC
TTTGAATCATTGTTATATTATTCACATTATCATTCACCTGAATATGTTAT
GCCGGATAAAAGTAGAAATACAAAAATTTAAATTCTTTAAAAAAAAA
back to top

protein sequence of SMED30003729-orf-1

>SMED30003729-orf-1 ID=SMED30003729-orf-1|Name=SMED30003729-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=319bp
MEFIKAFEEIDKDESGEITRGELEEYMKAKNYKPAFVEKWMRIFDIDNSG
TISLEEFCSTLGLIPGKKQKERIQRRNSQKQLELEAKSKKKSNEEDTQTS
NFSSSNSKVISKDITVIIENPNYTTTQSFNGLEKNNSIEIESRKESNLIE
NDKSKEIDKQDSNQNSRSHEQMISLSTSLDRTKTDGESKLSPVKCLNEIN
QKETETRETIEEYERMITRFKRIIIPSDEIYVISEDPDMTDSLRNFVLNL
SLDSARRFNEPLEMAKCIKRILDFTFNRSWHVVTGKDAYGCLVTHEEGRF
IHVRAREWGILMWQIFKT*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR037177DLC_sf
IPR001372Dynein_light_chain_typ-1/2
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0006810transport
GO:0030473nuclear migration along microtubule
GO:0040001establishment of mitotic spindle localization
GO:0051292nuclear pore complex assembly
GO:2000582positive regulation of ATP-dependent microtubule motor activity, plus-end-directed
Vocabulary: cellular component
TermDefinition
GO:0030286dynein complex
GO:0005643nuclear pore
GO:0005737cytoplasm
GO:0005777peroxisome
GO:0005856cytoskeleton
GO:0005868cytoplasmic dynein complex
GO:0005874microtubule
GO:0005875microtubule associated complex
GO:0005881cytoplasmic microtubule
GO:1990429peroxisomal importomer complex
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0003774motor activity
GO:0008574ATP-dependent microtubule motor activity, plus-end-directed
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 196..216
NoneNo IPR availableCOILSCoilCoilcoord: 70..90
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..109
e-value: 4.0E-16
score: 60.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..165
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 166..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..96
IPR001372Dynein light chain, type 1/2SMARTSM01375Dynein_light_2coord: 227..315
e-value: 1.4E-13
score: 61.1
IPR001372Dynein light chain, type 1/2PFAMPF01221Dynein_lightcoord: 237..314
e-value: 8.6E-15
score: 54.8
IPR002048EF-hand domainSMARTSM00054efh_1coord: 2..30
e-value: 9.5E-4
score: 28.5
coord: 36..64
e-value: 3.0E-4
score: 30.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 5..58
e-value: 5.2E-10
score: 39.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 37..67
score: 10.775
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 1..33
score: 12.449
IPR002048EF-hand domainCDDcd00051EFhcoord: 2..58
e-value: 1.0966E-12
score: 60.2541
IPR037177Dynein light chain superfamilyGENE3DG3DSA:3.30.740.10coord: 225..316
e-value: 3.2E-14
score: 54.7
IPR037177Dynein light chain superfamilySUPERFAMILYSSF54648DLCcoord: 233..314
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 11..23
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 45..57
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 2..65