SMED30003704
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003704 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30003704 aligns in the following genomic locations:
Homology
BLAST of SMED30003704 vs. Ensembl Human
Match: PYURF (PIGY upstream reading frame [Source:HGNC Symbol;Acc:HGNC:44317]) HSP 1 Score: 49.2914 bits (116), Expect = 4.808e-7 Identity = 24/45 (53.33%), Postives = 30/45 (66.67%), Query Frame = 2 Query: 194 DPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIII 328 DP L+FLVCPLSK L Y + EL E+ +AY I+DGIP +I Sbjct: 51 DPALLEFLVCPLSKKPLRYEASTNELINEELGIAYPIIDGIPNMI 95
BLAST of SMED30003704 vs. Ensembl Xenopus
Match: PYURF (PIGY upstream reading frame [Source:HGNC Symbol;Acc:HGNC:44317]) HSP 1 Score: 47.7506 bits (112), Expect = 3.230e-6 Identity = 24/48 (50.00%), Postives = 30/48 (62.50%), Query Frame = 2 Query: 194 DPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIARE 337 DP L FLVCPLS+ L Y EL E+ +AY IVDGIP +I ++ Sbjct: 90 DPTLLQFLVCPLSRKSLRYEESTNELINDELGIAYPIVDGIPNMIPQD 137
BLAST of SMED30003704 vs. Ensembl Mouse
Match: Pyurf (Pigy upstream reading frame [Source:MGI Symbol;Acc:MGI:1913709]) HSP 1 Score: 46.595 bits (109), Expect = 2.378e-6 Identity = 23/45 (51.11%), Postives = 28/45 (62.22%), Query Frame = 2 Query: 194 DPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIII 328 P L FLVCPLSK L Y + EL E+ +AY I+DGIP +I Sbjct: 49 HPALLQFLVCPLSKKPLRYEASTNELVNEELGIAYPIIDGIPNMI 93
BLAST of SMED30003704 vs. UniProt/SwissProt
Match: sp|Q2K364|Y3977_RHIEC (UPF0434 protein RHE_CH03977 OS=Rhizobium etli (strain CFN 42 / ATCC 51251) OX=347834 GN=RHE_CH03977 PE=3 SV=1) HSP 1 Score: 53.1434 bits (126), Expect = 2.330e-8 Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 2 Query: 188 RNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFD 352 R DP+ L+ LVCPLSK +LSY+ EL + Q+AY I DGIPI++ E D Sbjct: 7 RVDPKLLELLVCPLSKGRLSYDREHNELVSEKAQLAYPIRDGIPIMLVSEARRLD 61
BLAST of SMED30003704 vs. UniProt/SwissProt
Match: sp|B5ZTJ9|Y3773_RHILW (UPF0434 protein Rleg2_3773 OS=Rhizobium leguminosarum bv. trifolii (strain WSM2304) OX=395492 GN=Rleg2_3773 PE=3 SV=1) HSP 1 Score: 53.1434 bits (126), Expect = 2.653e-8 Identity = 27/55 (49.09%), Postives = 35/55 (63.64%), Query Frame = 2 Query: 188 RNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFD 352 R DP+ LD LVCPLSK +LSY+ EL + ++AY I DGIPI++ E D Sbjct: 7 RVDPKLLDLLVCPLSKGRLSYDREHNELVSEKARLAYPIRDGIPIMLVSEARRLD 61
BLAST of SMED30003704 vs. UniProt/SwissProt
Match: sp|B0UM25|Y487_METS4 (UPF0434 protein M446_0487 OS=Methylobacterium sp. (strain 4-46) OX=426117 GN=M446_0487 PE=3 SV=1) HSP 1 Score: 51.6026 bits (122), Expect = 8.819e-8 Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 2 Query: 185 VRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIARE 337 R DP+ L+ LVCPL+K +L Y+S ++EL ++AY I DGIPI++ E Sbjct: 10 TRIDPKLLELLVCPLTKGRLEYDSARQELISRSAKLAYPIRDGIPIMLPEE 60
BLAST of SMED30003704 vs. UniProt/SwissProt
Match: sp|B8IPV3|Y1613_METNO (UPF0434 protein Mnod_1613 OS=Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060) OX=460265 GN=Mnod_1613 PE=3 SV=1) HSP 1 Score: 51.6026 bits (122), Expect = 9.455e-8 Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 2 Query: 185 VRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIARE 337 R DP+ L+ LVCPL+K +L Y+S ++EL ++AY I DGIPI++ E Sbjct: 10 TRIDPKLLELLVCPLTKGRLEYDSARQELISRSAKLAYPIRDGIPIMLPEE 60
BLAST of SMED30003704 vs. UniProt/SwissProt
Match: sp|B3PR35|Y4260_RHIE6 (UPF0434 protein RHECIAT_CH0004260 OS=Rhizobium etli (strain CIAT 652) OX=491916 GN=RHECIAT_CH0004260 PE=3 SV=1) HSP 1 Score: 51.6026 bits (122), Expect = 1.047e-7 Identity = 26/55 (47.27%), Postives = 35/55 (63.64%), Query Frame = 2 Query: 188 RNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFD 352 R DP+ L+ LVCPLSK +LSY+ EL + ++AY I DGIPI++ E D Sbjct: 7 RVDPKLLELLVCPLSKGRLSYDREHNELVSEKARLAYPIRDGIPIMLVSEARRLD 61
BLAST of SMED30003704 vs. TrEMBL
Match: C1BGX7 (PreY, mitochondrial OS=Oncorhynchus mykiss OX=8022 GN=PREY PE=2 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 9.612e-8 Identity = 36/85 (42.35%), Postives = 47/85 (55.29%), Query Frame = 2 Query: 89 FCNAHLKLKD*LKY-HMIRTVFRYSSSFKHFPKVRN----DPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIII 328 CN L+ + KY H +R V S S +H V++ D L+FLVCPLSK QL Y EL E+ +AY I+DGIP +I Sbjct: 9 LCNETLRFQTTAKYVHFLRGVRPLSVSARHLTDVKDALPFDTSLLEFLVCPLSKKQLRYEEKTNELINEELGIAYPIIDGIPNMI 93
BLAST of SMED30003704 vs. TrEMBL
Match: A0A3D9Z315 (UPF0434 protein DES32_0709 OS=Methylovirgula ligni OX=569860 GN=DES32_0709 PE=3 SV=1) HSP 1 Score: 57.7658 bits (138), Expect = 2.086e-7 Identity = 28/59 (47.46%), Postives = 38/59 (64.41%), Query Frame = 2 Query: 179 PKVRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFDM 355 PK R DPR L+ LVCPL+K L Y++ K+EL ++AY I DGIPI++ E D+ Sbjct: 15 PKTRIDPRLLEILVCPLTKTTLEYDAQKQELISRAAKLAYPIRDGIPIMLPEEARPLDV 73
BLAST of SMED30003704 vs. TrEMBL
Match: A0A061AFP5 (Uncharacterized protein OS=Oncorhynchus mykiss OX=8022 GN=GSONMT00009444001 PE=3 SV=1) HSP 1 Score: 58.151 bits (139), Expect = 3.819e-7 Identity = 35/85 (41.18%), Postives = 48/85 (56.47%), Query Frame = 2 Query: 89 FCNAHLKLKD*LKY-HMIRTVFRYSSSFKHFPKVRN----DPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIII 328 CN L+ + +KY + +R V S S +H V++ D L+FLVCPLSK QL Y EL E+ +AY I+DGIP +I Sbjct: 9 LCNETLRFQTTVKYVNFLRGVRPLSVSARHLTDVKDALPFDTSLLEFLVCPLSKKQLRYEEKTNELINEELGIAYPIIDGIPNMI 93
BLAST of SMED30003704 vs. TrEMBL
Match: A0A0B1Q0V7 (UPF0434 protein LA66_19980 OS=Aureimonas altamirensis OX=370622 GN=LA66_19980 PE=3 SV=1) HSP 1 Score: 56.225 bits (134), Expect = 5.321e-7 Identity = 27/56 (48.21%), Postives = 37/56 (66.07%), Query Frame = 2 Query: 185 VRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFD 352 +R DP+ L+ LVCPL+K QLSY+S + EL + +AY I DGIPI++ E D Sbjct: 7 IRPDPKLLELLVCPLTKGQLSYDSERNELVSRKAGLAYPIRDGIPIMLPSEARILD 62
BLAST of SMED30003704 vs. TrEMBL
Match: C1BIF9 (PreY, mitochondrial OS=Oncorhynchus mykiss OX=8022 GN=PREY PE=2 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 6.878e-7 Identity = 34/85 (40.00%), Postives = 49/85 (57.65%), Query Frame = 2 Query: 89 FCNAHLKLKD*LKY-HMIRTVFRYSSSFKHFPKVRN----DPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIII 328 CN L+ + +KY + +R V S +H V++ D L+FLVCPLSK QL Y +EL E+++AY I+DGIP +I Sbjct: 9 LCNETLRFQTSVKYVNFLRGVRPLPVSARHLTDVKDALPFDTSLLEFLVCPLSKKQLRYEEKTDELINEELRIAYPIIDGIPNMI 93
BLAST of SMED30003704 vs. Ensembl Cavefish
Match: zgc:162634 (pep primary_assembly:Astyanax_mexicanus-2.0:24:26509090:26514451:1 gene:ENSAMXG00000036941.1 transcript:ENSAMXT00000040655.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:zgc:162634) HSP 1 Score: 46.595 bits (109), Expect = 1.607e-6 Identity = 28/68 (41.18%), Postives = 37/68 (54.41%), Query Frame = 2 Query: 134 MIRTVFRYSSSFKHFPKVRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIARE 337 ++ T R SS K R+ L LVCPLSK L YN EL E+ +AY I+DGIP +I ++ Sbjct: 17 LLNTQRRLSSGLKG--DDRSVESVLQLLVCPLSKTPLRYNKETNELINEELGIAYPIIDGIPNMIPQD 82
BLAST of SMED30003704 vs. Ensembl Nematostella
Match: EDO41571 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3Z0]) HSP 1 Score: 43.8986 bits (102), Expect = 5.045e-6 Identity = 24/60 (40.00%), Postives = 33/60 (55.00%), Query Frame = 2 Query: 182 KVRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFDMAN 361 K + D + LD LVCPLSK L YN+ EL EI VA+ I GIP ++ + + + Sbjct: 37 KQKFDVKLLDILVCPLSKKPLRYNAETNELISDEIGVAFSIEQGIPNLVPTDGKLLNTTD 96
BLAST of SMED30003704 vs. Ensembl Medaka
Match: zgc:162634 (PIGY upstream reading frame [Source:NCBI gene;Acc:101159718]) HSP 1 Score: 45.8246 bits (107), Expect = 3.637e-6 Identity = 22/44 (50.00%), Postives = 28/44 (63.64%), Query Frame = 2 Query: 206 LDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIARE 337 LD L CPLSK L YN EL E+ +AY I+DGIP +I ++ Sbjct: 54 LDVLACPLSKKPLRYNPDTNELINEELGIAYPIIDGIPNMIPQD 97
BLAST of SMED30003704 vs. Planmine SMEST
Match: SMESG000043698.1 (SMESG000043698.1) HSP 1 Score: 190.274 bits (482), Expect = 9.740e-62 Identity = 92/92 (100.00%), Postives = 92/92 (100.00%), Query Frame = 2 Query: 134 MIRTVFRYSSSFKHFPKVRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFDMANSFPFRILKNGEFKHPF 409 MIRTVFRYSSSFKHFPKVRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFDMANSFPFRILKNGEFKHPF Sbjct: 1 MIRTVFRYSSSFKHFPKVRNDPRRLDFLVCPLSKIQLSYNSFKEELECAEIQVAYKIVDGIPIIIAREDTCFDMANSFPFRILKNGEFKHPF 92 The following BLAST results are available for this feature:
BLAST of SMED30003704 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of SMED30003704 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30003704 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30003704 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30003704 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of SMED30003704 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of SMED30003704 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30003704 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30003704 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of SMED30003704 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30003704 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30003704 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30003704 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of SMED30003704 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003704 ID=SMED30003704|Name=SMED30003704|organism=Schmidtea mediterranea sexual|type=transcript|length=857bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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