SMED30003661
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003661 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30003661 aligns in the following genomic locations:
Homology
BLAST of SMED30003661 vs. Planmine SMEST
Match: SMESG000025366.1 (SMESG000025366.1) HSP 1 Score: 758.829 bits (1958), Expect = 0.000e+0 Identity = 469/495 (94.75%), Postives = 478/495 (96.57%), Query Frame = 1 Query: 145 MSDQVSRDILFKQELSVKLAYSLCSILFSIGVLADYSYYVLRVIEYGNYKDSIQRGFSYVILGXXXXXXXXXXXXTNLNYKYRNFRYISPIQRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNDKTDEMXXXXXXXXXXXXNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRXXXXXXXXXXXXYLIFTVACKTLEVLQRFNQNILILITTMKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKTSERNTRSIYVIYNSLILLENFLKSILLSLSLNRVPKKFKRKSWNAYLGSPFILMFSYIGIFCHQLISLPYPVNNTYQNKXXXXGIIHIGKPLAFLFDWMTILLFSSILIKFA 1629 MSDQVSRDILFKQELSVKL +SLCSILFSIGVLADYSYYVLRVIEYGNYKD IQRGFSYV LG+IVINIVIIIIN NLNYKYRNFRY PIQRPLILYLIYF LIIFSI GL S FIKLLLK +++TDEMVFSIIFGVFVVSNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYS++YKLSNCFISSINHHFNASF SKNGENSE+LFD EY ETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRITSTSGIIISGSYLIFTVACKTLEVLQ FNQNILILITTMKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGL+VIMDIVVDVFML TG DLKTSERNTRSIYVIYNSLILLENFLKSILLSLSLNRVPKKFKRKSWNAYLGSPFILMFSYIGIFCHQLISLPYPVNNTYQNKLLLLGIIHIGKPLAFLFDWMTILLFSSILIKFA Sbjct: 1 MSDQVSRDILFKQELSVKLTFSLCSILFSIGVLADYSYYVLRVIEYGNYKDRIQRGFSYVNLGMIVINIVIIIINKNLNYKYRNFRYRFPIQRPLILYLIYFLLIIFSIFGLTSYFIKLLLKHSNETDEMVFSIIFGVFVVSNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSFIYKLSNCFISSINHHFNASFFSKNGENSEFLFDPEYRETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRITSTSGIIISGSYLIFTVACKTLEVLQTFNQNILILITTMKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLSVIMDIVVDVFMLSTGLDLKTSERNTRSIYVIYNSLILLENFLKSILLSLSLNRVPKKFKRKSWNAYLGSPFILMFSYIGIFCHQLISLPYPVNNTYQNKLLLLGIIHIGKPLAFLFDWMTILLFSSILIKFA 495
BLAST of SMED30003661 vs. Planmine SMEST
Match: SMESG000025363.1 (SMESG000025363.1) HSP 1 Score: 629.787 bits (1623), Expect = 0.000e+0 Identity = 407/425 (95.76%), Postives = 415/425 (97.65%), Query Frame = 1 Query: 145 MSDQVSRDILFKQELSVKLAYSLCSILFSIGVLADYSYYVLRVIEYGNYKDSIQRGFSYVILGXXXXXXXXXXXXTNLNYKYRNFRYISPIQRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNDKTDEMXXXXXXXXXXXXNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRXXXXXXXXXXXXYLIFTVACKTLEVLQRFNQNILILITTMKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKTSERNTRSIYVIYNSLILLENFLKSILLSLSLNRVPKKFKRKS 1419 MSD+VSRDILFKQELSVKL +SLCSILFSIGVLADYSYYVLRVIEYGNYKD IQRGFSYV+LGI+VINIVIIIINTNLNYKYRNFRY PIQRPLILYLIYFPLIIFSIIGLISLFIKLLLKLNDKTDEMVFSIIFGVFVVSNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRITSTSGIIISGSYLIFTVACKTLEVLQRFNQNILILIT MKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKT+ERNTRSIYVIYNSLILLENFLKSILLSLSLNR +F+ + Sbjct: 1 MSDKVSRDILFKQELSVKLTFSLCSILFSIGVLADYSYYVLRVIEYGNYKDRIQRGFSYVMLGIVVINIVIIIINTNLNYKYRNFRYRFPIQRPLILYLIYFPLIIFSIIGLISLFIKLLLKLNDKTDEMVFSIIFGVFVVSNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRITSTSGIIISGSYLIFTVACKTLEVLQRFNQNILILITIMKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKTTERNTRSIYVIYNSLILLENFLKSILLSLSLNRFGDEFENEE 425
BLAST of SMED30003661 vs. Planmine SMEST
Match: SMESG000025362.1 (SMESG000025362.1) HSP 1 Score: 625.55 bits (1612), Expect = 0.000e+0 Identity = 409/435 (94.02%), Postives = 417/435 (95.86%), Query Frame = 1 Query: 145 MSDQVSRDILFKQELSVKLAYSLCSILFSIGVLADYSYYVLRVIEYGNYKDSIQRGFSYVILGXXXXXXXXXXXXTNLNYKYRNFRYISPIQRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNDKTDEMXXXXXXXXXXXXNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRXXXXXXXXXXXXYLIFTVACKTLEVLQRFNQNILILITTMKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKTSERNTRSIYVIYNSLILLENFLKSILLSLSLNRVPKKF---------KRKSW 1422 MSD+VSRDILFKQELSVKL +SLCSILFSIGVLADYSYYVLRVIEYGNYKD IQRGFSYV+LGI+VINIVIIIINTNLNYKYRNFRY PIQRPLILYLIYFPLIIFSIIGLISLFIKLLLKLNDKTDEMVFSIIFGVFVVSNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRITSTSGIIISGSYLIFTVACKTLEVLQRFNQNILILIT MKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKT+ERNTRSIYVIYNSLILLENFLKSILLSLSLNR K + K++ W Sbjct: 1 MSDKVSRDILFKQELSVKLTFSLCSILFSIGVLADYSYYVLRVIEYGNYKDRIQRGFSYVMLGIVVINIVIIIINTNLNYKYRNFRYRFPIQRPLILYLIYFPLIIFSIIGLISLFIKLLLKLNDKTDEMVFSIIFGVFVVSNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRITSTSGIIISGSYLIFTVACKTLEVLQRFNQNILILITIMKVLPIVVTILFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKTTERNTRSIYVIYNSLILLENFLKSILLSLSLNRKKKDYFGLRYSEKDKQRQW 435
BLAST of SMED30003661 vs. Planmine SMEST
Match: SMESG000025344.1 (SMESG000025344.1) HSP 1 Score: 102.834 bits (255), Expect = 5.095e-23 Identity = 122/481 (25.36%), Postives = 248/481 (51.56%), Query Frame = 1 Query: 178 KQELSVKLAYSLCSILFSIGVLADYSYYVLRVIEYGNYKDSIQRGFSYVILGXXXXXXXXXXXXTNLNYKYRNFRYISPIQRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNDKTDEMXXXXXXXXXXXXNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGN----QRXXXXXXXXXXXXYLIFTVACKTLEVLQRFNQNILILITTMKVLPIVVTI-------LFIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFT-GPDLKTSERNTRSIYVIYNSLI-LLENFLKSILLSLSLNRVPKKFKRKSWNAYLGSPFILMFSYIGIFCHQLISLPYPVNNTYQNKXXXXGIIHIGKPLAFLF 1581 ++E+ +K S+ LF + + +Y +YV ++ G+ KD +Y I + +++I++I NT +N+++RN R +++PLI YLI P++ S++GL + + + + +++ + ++LV+DI G DTGE WF+N+FN +TI++++ ++ + + L++ F +++N +F+S ++ +FD +T + ++I + +FI +LVIPL N+ +L R E++P+ +++S +G G +L F V L V+Q Q +I +++ ++V+ + I E++I + + +++K+ +IYLG FG I +IVV+++ L T G L S T+ I ++ ++ + L+E L + L+ L+ F + SW+ + SP ++ IG+F L Y N++Q +++ +G ++ PL L+ Sbjct: 11 RKEICLKFLISVPLFLFGMVYVTNYGFYVYQISPRGDRKDEYLDKINYSISFVKIVSIILICYNTQINHRFRNLRIKHKLEQPLIFYLICLPMLFISLMGLFVIIFHYTFEKSGTSTNILYFALVIFEYFLFIILVIDINGFSDTGEEWFMNYFNSMTIIITMTSVMIQICLNIIMILTDDFTNTMNR---TTFVSLFSNHNFTIFD----KTCYRTLVFLKKRAVITLSLVFIPFLVIPLKRNLTHLFRFKP----KEDLPEDKTVLSMNKVSSITGGFTVGVFLTFIVIVHLLYVMQNVPQ----VIIASRIIKLLVSFFISCLLGIMITEIKISARLNSSQYLNVKNILIYLGAFGQFSVQITNIVVNIYNLQTNGNGLFES---TKDILILTSNFVYLIETILMTTLMLFVLDGKAVSFPKNSWSIFFYSPINILILQIGVFTEYLTKSFYDEQNSFQIRMMEIGTMYFAIPLVILY 473
BLAST of SMED30003661 vs. Planmine SMEST
Match: SMESG000025369.1 (SMESG000025369.1) HSP 1 Score: 88.5817 bits (218), Expect = 2.558e-18 Identity = 123/506 (24.31%), Postives = 257/506 (50.79%), Query Frame = 1 Query: 124 RIFEKISMSDQVSRDILFKQELSVKLAYSLCSILFSIGVLADYSYYVLRVIEYGNYKDSIQRGFSYVILGXXXXXXXXXXXXTNLNYKYRNFRYISPIQRXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-XNDKTDEMXXXXXXXXXXXXNLVLVLDITGTKDTGELWFLNFFNFLTILVSIILFSLESSYSYVYKLSNCFISSINHHFNASFLSKNGENSEYLFDLEYTETALDWFADALENSLIIIVFIFISYLVIPLINNMKNLKRNISLKIFNENIPDQGNQRXXXXXXXXXXXXYLIFTVACKTLEVLQR--------FNQNILILITTMKVLPIVVTIL-FIKEMRIWSLDSEVSQVSLKSFIIYLGTFGYGLTVIMDIVVDVFMLFTGPDLKTSERNTRSIYVIYNSLILLENFLKSILLSLSLNRVPKKFKRKSWNAYLGSPFILMFSYIGIFCHQLISLPYPVNNTYQNKXXXXGIIHIGKPLAFLFDWMTILLFSS 1611 R + S+ Q +R+ + K +S+KL ++FSI L +YS +++R + I + + ++ + + NI++II N +NY++RN R +P++RP+ILYLI PL++ SII + + LK L+D D V + +++ ++ +LDI+G ++TGE W +++ NF I+++I +E+ + +K+S+ + + I +FN SF +N + +L T +A++ E+S+I+++ + YL++ L +MKN+ +++S N P + R + + + ++F++ L + + I +K + +V+ I+ I E+R+W E LK IY GTFG + ++ + ++F + + + + + Y+I + +++ L S L+ L L++ PK F + SW+ SP +L+ + + + + P ++ L+L+G +P+ ++ +++ +S Sbjct: 31 RTMPQDSIVTQSNRNAVIKFMISIKL------LIFSIVYLTNYSLFIVRFTPLKDRCIGIPKIITCAMVIVKLTNILLIIYNARINYRFRNLRIRNPVERPMILYLICLPLLVISIISISLSALNTTLKWLSDSIDMSVDFGCSILNLIAVILFILDISGCRNTGEEWLMSYVNFCIIIITICTIFMEAMKTLTFKMSSIYSNLIYKYFNISFYEENKFSKSFL-----TSSAINQIRVIEEDSIIMLLVVLFPYLIVCLDQHMKNMFKDVS----KGNNPQTLSVRNLTFLSLSGCITVVVFSIFELVLIIYTHGKPYTEWGCEEWIYRGFDGLKFIFVVINIVSMIHEIRLWLTVEENRFYDLKKLFIYFGTFGNIIKAVILVFFNIFQIVSQSVFENTTLSVNCSYIIMHVEDIIQTVLMSSLILLILDKEPKPFLKISWSYEFCSPILLLMNRVNEIIRCSLYMLIPNGKYFEINLILIGSYSFAQPIVIFYNVTCVIILAS 521 The following BLAST results are available for this feature:
BLAST of SMED30003661 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30003661 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30003661 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30003661 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30003661 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003661 ID=SMED30003661|Name=SMED30003661|organism=Schmidtea mediterranea sexual|type=transcript|length=1662bpback to top protein sequence of SMED30003661-orf-1 >SMED30003661-orf-1 ID=SMED30003661-orf-1|Name=SMED30003661-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=496bp MSDQVSRDILFKQELSVKLAYSLCSILFSIGVLADYSYYVLRVIEYGNYKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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