SMED30003656

Overview
NameSMED30003656
Smed IDSMED30003656
Length (bp)1552
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003656 (SMED30003656) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003656 (SMED30003656) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003656 (SMED30003656) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003656 (SMED30003656) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003656

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30003656 dd_Smed_v4_17687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30003656 vs. Ensembl Human
Match: ZBTB22 (zinc finger and BTB domain containing 22 [Source:HGNC Symbol;Acc:HGNC:13085])

HSP 1 Score: 55.4546 bits (132), Expect = 2.145e-7
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  484 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 532
[Close]
BLAST of SMED30003656 vs. Ensembl Human
Match: ZBTB22 (zinc finger and BTB domain containing 22 [Source:HGNC Symbol;Acc:HGNC:13085])

HSP 1 Score: 55.4546 bits (132), Expect = 2.145e-7
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  484 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 532
[Close]
BLAST of SMED30003656 vs. Ensembl Human
Match: ZBTB22 (zinc finger and BTB domain containing 22 [Source:HGNC Symbol;Acc:HGNC:13085])

HSP 1 Score: 55.4546 bits (132), Expect = 2.145e-7
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  484 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 532
[Close]
BLAST of SMED30003656 vs. Ensembl Human
Match: ZBTB22 (zinc finger and BTB domain containing 22 [Source:HGNC Symbol;Acc:HGNC:13085])

HSP 1 Score: 55.0694 bits (131), Expect = 2.163e-7
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  484 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 532
[Close]
BLAST of SMED30003656 vs. Ensembl Human
Match: ZBTB22 (zinc finger and BTB domain containing 22 [Source:HGNC Symbol;Acc:HGNC:13085])

HSP 1 Score: 55.0694 bits (131), Expect = 2.163e-7
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  484 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 532
[Close]
BLAST of SMED30003656 vs. Ensembl Celegans
Match: zag-1 (Zinc finger E-box-binding homeobox protein zag-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EBU4])

HSP 1 Score: 49.6766 bits (117), Expect = 4.453e-6
Identity = 21/50 (42.00%), Postives = 30/50 (60.00%), Query Frame = 1 
Query:  907 YLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            + C QC+  F   S+  RH   K+E+   RP++C +C K+FK  HHL EH
Sbjct:  481 FSCDQCDKVFGKQSSLARH---KYEHSGQRPYKCDICEKAFKHKHHLTEH 527
[Close]
BLAST of SMED30003656 vs. Ensembl Fly
Match: zfh1 (gene:FBgn0004606 transcript:FBtr0331180)

HSP 1 Score: 51.2174 bits (121), Expect = 2.599e-6
Identity = 23/50 (46.00%), Postives = 30/50 (60.00%), Query Frame = 1 
Query:  907 YLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            Y+C QC+  F   S+  RH   K+E+   RP+QC  C K+FK  HHL EH
Sbjct:  967 YVCDQCDKAFAKQSSLARH---KYEHSGQRPYQCIECPKAFKHKHHLTEH 1013
[Close]
BLAST of SMED30003656 vs. Ensembl Fly
Match: zfh1 (gene:FBgn0004606 transcript:FBtr0085701)

HSP 1 Score: 50.8322 bits (120), Expect = 2.804e-6
Identity = 23/50 (46.00%), Postives = 30/50 (60.00%), Query Frame = 1 
Query:  907 YLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            Y+C QC+  F   S+  RH   K+E+   RP+QC  C K+FK  HHL EH
Sbjct:  967 YVCDQCDKAFAKQSSLARH---KYEHSGQRPYQCIECPKAFKHKHHLTEH 1013
[Close]
BLAST of SMED30003656 vs. Ensembl Fly
Match: zfh1 (gene:FBgn0004606 transcript:FBtr0085702)

HSP 1 Score: 50.447 bits (119), Expect = 3.669e-6
Identity = 23/50 (46.00%), Postives = 30/50 (60.00%), Query Frame = 1 
Query:  907 YLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            Y+C QC+  F   S+  RH   K+E+   RP+QC  C K+FK  HHL EH
Sbjct:  660 YVCDQCDKAFAKQSSLARH---KYEHSGQRPYQCIECPKAFKHKHHLTEH 706
[Close]
BLAST of SMED30003656 vs. Ensembl Zebrafish
Match: zbtb22b (zinc finger and BTB domain containing 22b [Source:ZFIN;Acc:ZDB-GENE-010110-4])

HSP 1 Score: 55.0694 bits (131), Expect = 1.581e-7
Identity = 24/59 (40.68%), Postives = 35/59 (59.32%), Query Frame = 1 
Query:  883 TTGIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            + G   K ++C  C   F + S R+RH+++   + N RPF C VC K FK+ HHL EH+
Sbjct:  480 SAGSSGKVFMC-HCGKTFTHKSMRDRHINM---HLNLRPFHCPVCAKKFKMKHHLTEHM 534
[Close]
BLAST of SMED30003656 vs. Ensembl Zebrafish
Match: zbtb22a (zinc finger and BTB domain containing 22a [Source:ZFIN;Acc:ZDB-GENE-101105-1])

HSP 1 Score: 51.9878 bits (123), Expect = 1.202e-6
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K + C  C   +   S R+RHV ++H   N RPF C VC KSFK+ HHL +H+
Sbjct:  372 KVHFC-HCGKAYTLKSMRDRHVKMQH--LNLRPFSCPVCSKSFKMKHHLTKHV 421
[Close]
BLAST of SMED30003656 vs. Ensembl Zebrafish
Match: zeb2a (zinc finger E-box binding homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-070912-553])

HSP 1 Score: 51.2174 bits (121), Expect = 3.200e-6
Identity = 26/67 (38.81%), Postives = 37/67 (55.22%), Query Frame = 1 
Query:  856 VATRNNKEITTGIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            +A +  K+  +G+    Y C  C+  F   S+  RH   K+E+   RP QCQ+C K+FK  HHL EH
Sbjct:  987 LARKKIKKTESGM----YACDLCDKTFQKTSSLLRH---KYEHTGKRPHQCQICKKAFKHKHHLIEH 1046
[Close]
BLAST of SMED30003656 vs. Ensembl Zebrafish
Match: zeb2a (zinc finger E-box binding homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-070912-553])

HSP 1 Score: 51.2174 bits (121), Expect = 3.200e-6
Identity = 26/67 (38.81%), Postives = 37/67 (55.22%), Query Frame = 1 
Query:  856 VATRNNKEITTGIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            +A +  K+  +G+    Y C  C+  F   S+  RH   K+E+   RP QCQ+C K+FK  HHL EH
Sbjct:  987 LARKKIKKTESGM----YACDLCDKTFQKTSSLLRH---KYEHTGKRPHQCQICKKAFKHKHHLIEH 1046
[Close]
BLAST of SMED30003656 vs. Ensembl Zebrafish
Match: zeb2a (zinc finger E-box binding homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-070912-553])

HSP 1 Score: 51.2174 bits (121), Expect = 3.200e-6
Identity = 26/67 (38.81%), Postives = 37/67 (55.22%), Query Frame = 1 
Query:  856 VATRNNKEITTGIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            +A +  K+  +G+    Y C  C+  F   S+  RH   K+E+   RP QCQ+C K+FK  HHL EH
Sbjct:  987 LARKKIKKTESGM----YACDLCDKTFQKTSSLLRH---KYEHTGKRPHQCQICKKAFKHKHHLIEH 1046
[Close]
BLAST of SMED30003656 vs. Ensembl Xenopus
Match: zbtb6 (zinc finger and BTB domain containing 6 [Source:NCBI gene;Acc:100037884])

HSP 1 Score: 49.6766 bits (117), Expect = 8.709e-6
Identity = 23/53 (43.40%), Postives = 29/53 (54.72%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC QC   F    N NRH+     +   RPFQC VCLK+F     L++HL
Sbjct:  313 KLFLCLQCGKTFTQKKNLNRHIR---GHMGIRPFQCSVCLKTFTAKSTLQDHL 362
[Close]
BLAST of SMED30003656 vs. Ensembl Mouse
Match: Zbtb22 (zinc finger and BTB domain containing 22 [Source:MGI Symbol;Acc:MGI:1931870])

HSP 1 Score: 54.6842 bits (130), Expect = 2.219e-7
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  481 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 529
[Close]
BLAST of SMED30003656 vs. Ensembl Mouse
Match: Zbtb6 (zinc finger and BTB domain containing 6 [Source:MGI Symbol;Acc:MGI:2442998])

HSP 1 Score: 49.2914 bits (116), Expect = 8.477e-6
Identity = 23/53 (43.40%), Postives = 29/53 (54.72%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC QC   F    N NRH+     +   RPFQC VCLK+F     L++HL
Sbjct:  323 KLFLCLQCGKTFTQKKNLNRHIR---GHMGIRPFQCTVCLKTFTAKSTLQDHL 372
[Close]
BLAST of SMED30003656 vs. Ensembl Mouse
Match: Zbtb6 (zinc finger and BTB domain containing 6 [Source:MGI Symbol;Acc:MGI:2442998])

HSP 1 Score: 49.2914 bits (116), Expect = 8.477e-6
Identity = 23/53 (43.40%), Postives = 29/53 (54.72%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC QC   F    N NRH+     +   RPFQC VCLK+F     L++HL
Sbjct:  323 KLFLCLQCGKTFTQKKNLNRHIR---GHMGIRPFQCTVCLKTFTAKSTLQDHL 372
[Close]
BLAST of SMED30003656 vs. UniProt/SwissProt
Match: sp|O15209|ZBT22_HUMAN (Zinc finger and BTB domain-containing protein 22 OS=Homo sapiens OX=9606 GN=ZBTB22 PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.039e-6
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  484 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 532
[Close]
BLAST of SMED30003656 vs. UniProt/SwissProt
Match: sp|Q5TJE2|ZBT22_CANLF (Zinc finger and BTB domain-containing protein 22 OS=Canis lupus familiaris OX=9615 GN=ZBTB22 PE=3 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.230e-6
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  488 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 536
[Close]
BLAST of SMED30003656 vs. UniProt/SwissProt
Match: sp|Q9Z0G7|ZBT22_MOUSE (Zinc finger and BTB domain-containing protein 22 OS=Mus musculus OX=10090 GN=Zbtb22 PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.552e-6
Identity = 25/53 (47.17%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K +LC  C   F++ S R+RHV++   + N RPF C VC K FK+ HHL EH+
Sbjct:  481 KIFLC-HCGKAFSHKSMRDRHVNM---HLNLRPFDCPVCNKKFKMKHHLTEHM 529
[Close]
BLAST of SMED30003656 vs. Ensembl Cavefish
Match: zbtb22b (zinc finger and BTB domain containing 22b [Source:ZFIN;Acc:ZDB-GENE-010110-4])

HSP 1 Score: 55.0694 bits (131), Expect = 1.233e-7
Identity = 23/53 (43.40%), Postives = 34/53 (64.15%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K ++C  C  KF + S R+RH+++   + + RPF C VC K FK+ HHL EH+
Sbjct:  484 KVFMC-HCGKKFTHKSMRDRHINM---HLDLRPFHCPVCAKKFKMKHHLTEHM 532
[Close]
BLAST of SMED30003656 vs. Ensembl Cavefish
Match: zbtb22a (zinc finger and BTB domain containing 22a [Source:ZFIN;Acc:ZDB-GENE-101105-1])

HSP 1 Score: 53.9138 bits (128), Expect = 3.019e-7
Identity = 25/53 (47.17%), Postives = 32/53 (60.38%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K + C  C   F   S R+RHV ++H   N RPF C VC KSFK+ HHL +H+
Sbjct:  433 KVHFC-HCGKAFTLKSMRDRHVKMQH--LNLRPFACPVCAKSFKMKHHLTKHV 482
[Close]
BLAST of SMED30003656 vs. Ensembl Cavefish
Match: zbtb43 (zinc finger and BTB domain containing 43 [Source:NCBI gene;Acc:103027140])

HSP 1 Score: 52.373 bits (124), Expect = 7.491e-7
Identity = 28/72 (38.89%), Postives = 37/72 (51.39%), Query Frame = 1 
Query:  859 ATRNNKEITT-----GIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
               + KE TT      +  K Y C QC   F + S R+RH+ +   +   RPF C VC KSFK+ HHL  H+
Sbjct:  336 GGEDTKEQTTHSGFASVVYKLYPC-QCGKSFTHKSQRDRHMSM---HLGLRPFGCAVCGKSFKMKHHLVGHM 403
[Close]
BLAST of SMED30003656 vs. Ensembl Cavefish
Match: zeb2a (zinc finger E-box binding homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-070912-553])

HSP 1 Score: 51.2174 bits (121), Expect = 2.853e-6
Identity = 26/67 (38.81%), Postives = 37/67 (55.22%), Query Frame = 1 
Query:  856 VATRNNKEITTGIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            +A +  K+  +G+    Y C  C+  F   S+  RH   K+E+   RP QCQ+C K+FK  HHL EH
Sbjct:  969 LARKKIKKTESGM----YACDLCDKTFQKTSSLLRH---KYEHTGKRPHQCQICKKAFKHKHHLIEH 1028
[Close]
BLAST of SMED30003656 vs. Ensembl Cavefish
Match: zeb2a (zinc finger E-box binding homeobox 2a [Source:ZFIN;Acc:ZDB-GENE-070912-553])

HSP 1 Score: 50.8322 bits (120), Expect = 3.686e-6
Identity = 26/67 (38.81%), Postives = 37/67 (55.22%), Query Frame = 1 
Query:  856 VATRNNKEITTGIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEH 1056
            +A +  K+  +G+    Y C  C+  F   S+  RH   K+E+   RP QCQ+C K+FK  HHL EH
Sbjct: 1149 LARKKIKKTESGM----YACDLCDKTFQKTSSLLRH---KYEHTGKRPHQCQICKKAFKHKHHLIEH 1208
[Close]
BLAST of SMED30003656 vs. Ensembl Sea Lamprey
Match: zbtb43 (zinc finger and BTB domain containing 43 [Source:NCBI gene;Acc:557590])

HSP 1 Score: 51.9878 bits (123), Expect = 2.161e-7
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = 1 
Query:  886 TGIKIKTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
             G   K Y C QC   F + S R+RH+ +   +   RP+ C +C KSFK+ HHL  H+
Sbjct:  376 AGSACKLYQC-QCGKGFTHRSQRDRHMSM---HLGLRPYSCSICGKSFKMKHHLSGHM 429
[Close]
BLAST of SMED30003656 vs. Ensembl Medaka
Match: zbtb22a (zinc finger and BTB domain-containing protein 22 [Source:NCBI gene;Acc:101164416])

HSP 1 Score: 53.1434 bits (126), Expect = 4.628e-7
Identity = 25/53 (47.17%), Postives = 32/53 (60.38%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K + C  C   +   S R+RHV ++H   N RPF C VC KSFK+ HHL +HL
Sbjct:  469 KVHFC-HCGKAYTLKSMRDRHVKMQH--LNLRPFGCPVCSKSFKMKHHLTKHL 518
[Close]
BLAST of SMED30003656 vs. Ensembl Medaka
Match: zbtb22b (zinc finger and BTB domain-containing protein 22 [Source:NCBI gene;Acc:101155366])

HSP 1 Score: 53.5286 bits (127), Expect = 4.870e-7
Identity = 22/53 (41.51%), Postives = 33/53 (62.26%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K ++C  C   F + S R+RH+++   + + RPF C VC K FK+ HHL EH+
Sbjct:  533 KVFMC-HCGKSFTHKSMRDRHINM---HLDLRPFHCPVCAKKFKMKHHLTEHM 581
[Close]
BLAST of SMED30003656 vs. Ensembl Medaka
Match: zbtb22b (zinc finger and BTB domain-containing protein 22 [Source:NCBI gene;Acc:101155366])

HSP 1 Score: 53.1434 bits (126), Expect = 5.803e-7
Identity = 22/53 (41.51%), Postives = 33/53 (62.26%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K ++C  C   F + S R+RH+++   + + RPF C VC K FK+ HHL EH+
Sbjct:  538 KVFMC-HCGKSFTHKSMRDRHINM---HLDLRPFHCPVCAKKFKMKHHLTEHM 586
[Close]
BLAST of SMED30003656 vs. Ensembl Medaka
Match: zbtb22a (zinc finger and BTB domain-containing protein 22 [Source:NCBI gene;Acc:101164416])

HSP 1 Score: 51.9878 bits (123), Expect = 7.623e-7
Identity = 25/53 (47.17%), Postives = 32/53 (60.38%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K + C  C   +   S R+RHV ++H   N RPF C VC KSFK+ HHL +HL
Sbjct:  284 KVHFC-HCGKAYTLKSMRDRHVKMQH--LNLRPFGCPVCSKSFKMKHHLTKHL 333
[Close]
BLAST of SMED30003656 vs. Ensembl Medaka
Match: zbtb22a (zinc finger and BTB domain-containing protein 22 [Source:NCBI gene;Acc:101164416])

HSP 1 Score: 50.8322 bits (120), Expect = 2.572e-6
Identity = 25/53 (47.17%), Postives = 32/53 (60.38%), Query Frame = 1 
Query:  901 KTYLCSQCNAKFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHL 1059
            K + C  C   +   S R+RHV ++H   N RPF C VC KSFK+ HHL +HL
Sbjct:  275 KVHFC-HCGKAYTLKSMRDRHVKMQH--LNLRPFGCPVCSKSFKMKHHLTKHL 324
[Close]
BLAST of SMED30003656 vs. Planmine SMEST
Match: SMESG000067690.1 (SMESG000067690.1)

HSP 1 Score: 401.749 bits (1031), Expect = 3.711e-138
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 1 
Query:  109 MNYFENLLHGLECSTDKHCLIINVSFNDLPMDAFMECDTIEVTSNSDSNFNNRDXXXXXXXXXXXXXXXXXXXXXXXEPVSEAISTFTVKQEKDVLPSNAYNFDELPQEILNVPENYDSLNSAVSLPVSGNDCELSNESPGERGKFSPSKHQNQFISESPEDQALMSIENGCSILSSLIFNEPIEXXXXXXXXXXXXXXXXXPLQSSNANHLTIMNNFSLSHSNNVFPEINNLDLVSNNHTNSIPDHL 852
            MNYFENLLHGLECSTDKHCLIINVSFNDLPMDAFMECDTIEVTSNSDSNFNNRDKIKVKSKTNSKSKRNKNSRIINIEPVSEAISTFTVKQEKDVLPSNAYNFDELPQEILNVPENYDSLNSAVSLPVSGNDCELSNESPGERGKFSPSKHQNQFISESPEDQALMSIENGCSILSSLIFNEPIESFNSSNINLKSLSSPNIPLQSSNANHLTIMNNFSLSHSNNVFPEINNLDLVSNNHTNSIPD+L
Sbjct:    1 MNYFENLLHGLECSTDKHCLIINVSFNDLPMDAFMECDTIEVTSNSDSNFNNRDKIKVKSKTNSKSKRNKNSRIINIEPVSEAISTFTVKQEKDVLPSNAYNFDELPQEILNVPENYDSLNSAVSLPVSGNDCELSNESPGERGKFSPSKHQNQFISESPEDQALMSIENGCSILSSLIFNEPIESFNSSNINLKSLSSPNIPLQSSNANHLTIMNNFSLSHSNNVFPEINNLDLVSNNHTNSIPDYL 248

HSP 2 Score: 150.984 bits (380), Expect = 2.480e-41
Identity = 67/67 (100.00%), Postives = 67/67 (100.00%), Query Frame = 3 
Query: 1215 DYLPQIDERNLTVHRRFMYNNYMFVYHRKMKDGMVHWRCKNHGKTKCRARLHTRLGKIVKLPLCHNH 1415
            DYLPQIDERNLTVHRRFMYNNYMFVYHRKMKDGMVHWRCKNHGKTKCRARLHTRLGKIVKLPLCHNH
Sbjct:  246 DYLPQIDERNLTVHRRFMYNNYMFVYHRKMKDGMVHWRCKNHGKTKCRARLHTRLGKIVKLPLCHNH 312
The following BLAST results are available for this feature:
BLAST of SMED30003656 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ZBTB222.145e-747.17zinc finger and BTB domain containing 22 [Source:H... [more]
ZBTB222.145e-747.17zinc finger and BTB domain containing 22 [Source:H... [more]
ZBTB222.145e-747.17zinc finger and BTB domain containing 22 [Source:H... [more]
ZBTB222.163e-747.17zinc finger and BTB domain containing 22 [Source:H... [more]
ZBTB222.163e-747.17zinc finger and BTB domain containing 22 [Source:H... [more]
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BLAST of SMED30003656 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zag-14.453e-642.00Zinc finger E-box-binding homeobox protein zag-1 ... [more]
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BLAST of SMED30003656 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
zfh12.599e-646.00gene:FBgn0004606 transcript:FBtr0331180[more]
zfh12.804e-646.00gene:FBgn0004606 transcript:FBtr0085701[more]
zfh13.669e-646.00gene:FBgn0004606 transcript:FBtr0085702[more]
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BLAST of SMED30003656 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zbtb22b1.581e-740.68zinc finger and BTB domain containing 22b [Source:... [more]
zbtb22a1.202e-645.28zinc finger and BTB domain containing 22a [Source:... [more]
zeb2a3.200e-638.81zinc finger E-box binding homeobox 2a [Source:ZFIN... [more]
zeb2a3.200e-638.81zinc finger E-box binding homeobox 2a [Source:ZFIN... [more]
zeb2a3.200e-638.81zinc finger E-box binding homeobox 2a [Source:ZFIN... [more]
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BLAST of SMED30003656 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zbtb68.709e-643.40zinc finger and BTB domain containing 6 [Source:NC... [more]
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BLAST of SMED30003656 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Zbtb222.219e-747.17zinc finger and BTB domain containing 22 [Source:M... [more]
Zbtb68.477e-643.40zinc finger and BTB domain containing 6 [Source:MG... [more]
Zbtb68.477e-643.40zinc finger and BTB domain containing 6 [Source:MG... [more]
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BLAST of SMED30003656 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|O15209|ZBT22_HUMAN1.039e-647.17Zinc finger and BTB domain-containing protein 22 O... [more]
sp|Q5TJE2|ZBT22_CANLF1.230e-647.17Zinc finger and BTB domain-containing protein 22 O... [more]
sp|Q9Z0G7|ZBT22_MOUSE1.552e-647.17Zinc finger and BTB domain-containing protein 22 O... [more]
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BLAST of SMED30003656 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003656 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zbtb22b1.233e-743.40zinc finger and BTB domain containing 22b [Source:... [more]
zbtb22a3.019e-747.17zinc finger and BTB domain containing 22a [Source:... [more]
zbtb437.491e-738.89zinc finger and BTB domain containing 43 [Source:N... [more]
zeb2a2.853e-638.81zinc finger E-box binding homeobox 2a [Source:ZFIN... [more]
zeb2a3.686e-638.81zinc finger E-box binding homeobox 2a [Source:ZFIN... [more]
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BLAST of SMED30003656 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zbtb432.161e-739.66zinc finger and BTB domain containing 43 [Source:N... [more]
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BLAST of SMED30003656 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003656 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003656 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zbtb22a4.628e-747.17zinc finger and BTB domain-containing protein 22 [... [more]
zbtb22b4.870e-741.51zinc finger and BTB domain-containing protein 22 [... [more]
zbtb22b5.803e-741.51zinc finger and BTB domain-containing protein 22 [... [more]
zbtb22a7.623e-747.17zinc finger and BTB domain-containing protein 22 [... [more]
zbtb22a2.572e-647.17zinc finger and BTB domain-containing protein 22 [... [more]
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BLAST of SMED30003656 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000067690.13.711e-13899.60SMESG000067690.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003656 ID=SMED30003656|Name=SMED30003656|organism=Schmidtea mediterranea sexual|type=transcript|length=1552bp
TGAAAATTTTATATAATTTTTTTTCTAAATATGCTTTTCTTCACAAAAGA
GGAATTAAAAAGTCGAATATCTTGTATTAAACAAATGATGGATGCTCTAA
ACATTAAAATGAATTATTTTGAGAATTTATTGCACGGCTTAGAGTGTTCA
ACAGATAAACATTGTTTAATAATAAATGTTTCATTTAATGATTTACCAAT
GGATGCTTTCATGGAATGTGACACAATAGAAGTTACTTCTAATTCAGATA
GCAATTTTAACAATAGGGATAAAATAAAAGTCAAATCTAAAACTAATTCA
AAGAGTAAACGCAATAAAAATTCTCGCATTATAAATATTGAGCCTGTATC
TGAAGCAATTTCAACTTTTACAGTCAAGCAAGAGAAAGATGTTTTACCAT
CAAATGCATATAATTTCGATGAATTACCCCAAGAAATTTTAAATGTTCCA
GAAAACTATGACTCGTTAAATAGTGCTGTTTCTTTGCCAGTGTCAGGTAA
TGATTGTGAACTATCTAATGAATCGCCGGGGGAACGTGGTAAGTTCAGTC
CTTCAAAACACCAAAATCAATTCATCAGTGAATCACCAGAGGATCAGGCT
TTAATGTCTATAGAAAATGGATGTTCTATTTTAAGTTCTTTAATATTCAA
TGAACCAATTGAAAGTTTTAACTCTTCAAATATAAATCTGAAGTCATTAT
CTTCTCCAAATATTCCATTGCAATCTTCAAATGCCAATCATTTAACAATC
ATGAATAATTTTTCTTTATCTCATTCTAATAATGTATTTCCTGAAATCAA
TAATCTTGATTTGGTGTCAAATAACCACACAAATTCTATCCCAGATCATC
TTCATGTAGCAACAAGAAATAACAAAGAAATAACCACTGGTATTAAAATT
AAGACTTATTTGTGTTCCCAGTGCAATGCCAAATTTAATAATCTTTCAAA
TAGAAATAGGCATGTCCATTTGAAACACGAGTATTTTAACAATAGACCTT
TCCAGTGTCAGGTGTGCCTGAAATCCTTTAAATTGCATCATCATTTGAAA
GAACATTTATCCAGATGTGTTAATGATGTGATCAATATGACTATTAGTGA
TTAATCTATATATATATTGGTTGCCAAGTTTTATTCTATTAAATTTTTAT
TGTGAGCTTACAATCGAATTTGTAATTTTTTTCACAATTTAAATTTCGAT
TTGTTGTCTATTTAGACTATCTACCCCAAATTGATGAAAGAAATTTGACT
GTACACCGAAGATTTATGTATAACAATTATATGTTTGTTTATCATCGAAA
AATGAAAGATGGCATGGTTCACTGGAGATGTAAAAATCACGGTAAAACAA
AATGTCGTGCAAGATTGCATACTCGACTGGGGAAAATTGTTAAACTTCCT
TTATGTCATAATCATTAATTTATCATTTATTGTCGATTATTTTTTGTATA
TATTTTTAAGCAACATTTGAAAAATAATTTTTCTGCTTTTGATTGATGAT
ATCTTAGTAATAAAAGTTTTTGTGTGAATTGAAATATATATATATATATA
TA
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protein sequence of SMED30003656-orf-1

>SMED30003656-orf-1 ID=SMED30003656-orf-1|Name=SMED30003656-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=358bp
MLFFTKEELKSRISCIKQMMDALNIKMNYFENLLHGLECSTDKHCLIINV
SFNDLPMDAFMECDTIEVTSNSDSNFNNRDKIKVKSKTNSKSKRNKNSRI
INIEPVSEAISTFTVKQEKDVLPSNAYNFDELPQEILNVPENYDSLNSAV
SLPVSGNDCELSNESPGERGKFSPSKHQNQFISESPEDQALMSIENGCSI
LSSLIFNEPIESFNSSNINLKSLSSPNIPLQSSNANHLTIMNNFSLSHSN
NVFPEINNLDLVSNNHTNSIPDHLHVATRNNKEITTGIKIKTYLCSQCNA
KFNNLSNRNRHVHLKHEYFNNRPFQCQVCLKSFKLHHHLKEHLSRCVNDV
INMTISD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR007588Znf_FLYWCH
IPR015880Znf_C2H2-like
IPR013087Znf_C2H2_type
IPR007087Znf_C2H2
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046872metal ion binding
GO:0000977RNA polymerase II regulatory region sequence-specific DNA binding
GO:0003677DNA binding
GO:0003705transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
GO:0043565sequence-specific DNA binding
Vocabulary: biological process
TermDefinition
GO:0000122negative regulation of transcription from RNA polymerase II promoter
GO:0006355regulation of transcription, DNA-templated
GO:0007280pole cell migration
GO:0007399nervous system development
GO:0007498mesoderm development
GO:0007507heart development
GO:0007516hemocyte development
GO:0008045motor neuron axon guidance
GO:0008354germ cell migration
GO:0008406gonad development
GO:0019730antimicrobial humoral response
GO:0048103somatic stem cell division
GO:0048542lymph gland development
GO:0061321garland nephrocyte differentiation
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus