Ras-like C3 botulinum toxin substrate 1

Overview
NameRas-like C3 botulinum toxin substrate 1
Smed IDSMED30003600
Length (bp)1113
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ras-like C3 botulinum toxin substrate 1 (SMED30003600) t-SNE clustered cells

Violin plots show distribution of expression levels for Ras-like C3 botulinum toxin substrate 1 (SMED30003600) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ras-like C3 botulinum toxin substrate 1 (SMED30003600) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ras-like C3 botulinum toxin substrate 1 (SMED30003600) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003600

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30003600h1SMcG0011310 Contig2622GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003600h1SMcG0011310 Contig2622GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30003600h1SMcG0022019 Contig2622GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003600h1SMcG0022019 Contig2622GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Human
Match: RAC1 (Rac family small GTPase 1 [Source:HGNC Symbol;Acc:HGNC:9801])

HSP 1 Score: 231.491 bits (589), Expect = 7.674e-75
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Human
Match: RAC2 (Rac family small GTPase 2 [Source:HGNC Symbol;Acc:HGNC:9802])

HSP 1 Score: 230.72 bits (587), Expect = 1.604e-74
Identity = 109/192 (56.77%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPK-CSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   +YENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL LAK+I++VK++ECSA ++ GL  VF+EAIRAV+   P R++ + CSLL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWFPEVRHHCPSTPIILVGTKLDLRDDKDTIEKLKEKKLAPITYPQGLALAKEIDSVKYLECSALTQRGLKTVFDEAIRAVLCPQPTRQQKRACSLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Human
Match: RAC3 (Rac family small GTPase 3 [Source:HGNC Symbol;Acc:HGNC:9803])

HSP 1 Score: 219.935 bits (559), Expect = 2.671e-70
Identity = 100/177 (56.50%), Postives = 132/177 (74.58%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVI 606
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PI+LVGTK+DLR             ++ +TY +GL +A++I +VK++ECSA ++ GL  VF+EAIRAV+
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPHTPILLVGTKLDLRDDKDTIERLRDKKLAPITYPQGLAMAREIGSVKYLECSALTQRGLKTVFDEAIRAVL 177          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Human
Match: RAC1 (Rac family small GTPase 1 [Source:HGNC Symbol;Acc:HGNC:9801])

HSP 1 Score: 220.705 bits (561), Expect = 2.858e-70
Identity = 110/211 (52.13%), Postives = 141/211 (66.82%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNT-------------------SVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T                    VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTVGETYGKDITSRGKDKPIADVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 211          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Human
Match: RHOG (ras homolog family member G [Source:HGNC Symbol;Acc:HGNC:672])

HSP 1 Score: 218.394 bits (555), Expect = 9.077e-70
Identity = 101/191 (52.88%), Postives = 136/191 (71.20%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M++IKCVVVGDG VGKTC LI YTTN FP EY+PT+FDN+ A   VDG  +NL+LWDTAGQE YD+LR   Y  T+VF++CFS+    +YENV++ W  E+ ++CP +PI+LVGTK DLR              + +T Q+G  LAKQI+AV+++ECSA  ++G+ +VF EA+RAV+N TP ++   C LL
Sbjct:    1 MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQSAVDGRTVNLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCPDVPILLVGTKKDLRAQPDTLRRLKEQGQAPITPQQGQALAKQIHAVRYLECSALQQDGVKEVFAEAVRAVLNPTPIKRGRSCILL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Celegans
Match: ced-10 (Ras-related protein ced-10 [Source:UniProtKB/Swiss-Prot;Acc:Q03206])

HSP 1 Score: 226.483 bits (576), Expect = 3.031e-73
Identity = 107/191 (56.02%), Postives = 137/191 (71.73%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  INL LWDTAGQE YD+LR   Y  T VFLVCF++ +  ++ENV+  W  E+ ++CP  PIILVGTK DLR             +  V+  +G ++AK+I AVK++ECSA ++ GL QVF+EAIRAV+    + KK KC++L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGRPINLGLWDTAGQEDYDRLRPLSYPQTDVFLVCFALNNPASFENVRAKWYPEVSHHCPNTPIILVGTKADLREDRDTVERLRERRLQPVSQTQGYVMAKEIKAVKYLECSALTQRGLKQVFDEAIRAVLTPPQRAKKSKCTVL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Celegans
Match: mig-2 (Rac-like GTPase [Source:UniProtKB/TrEMBL;Acc:G5EBV3])

HSP 1 Score: 217.624 bits (553), Expect = 8.892e-70
Identity = 97/195 (49.74%), Postives = 141/195 (72.31%), Query Frame = 1
Query:   64 LKSHMKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            + S  + IKCVVVGDG VGKTC LISYTT+ FP +YVPT+FDN+ A + +DG  +NL LWDTAGQE YD+LR   Y  T VF++CFSV+   +++NV   W  EIR +CP  P+ILVGTK+DLR  A       +   S ++  +G+ +A++I AVK++ECSA +++GL+QVFE+A+R++++  P++KK  C+++
Sbjct:    1 MSSPSRQIKCVVVGDGTVGKTCMLISYTTDSFPVQYVPTVFDNYSAQMSLDGNVVNLGLWDTAGQEDYDRLRPLSYPQTDVFILCFSVVSPVSFDNVASKWIPEIRQHCPDAPVILVGTKLDLRDEAEPMRALQAEGKSPISKTQGMKMAQKIKAVKYLECSALTQQGLTQVFEDAVRSILHPKPQKKKKSCNIM 195          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Celegans
Match: rac-2 (Ras-related protein rac-2 [Source:UniProtKB/Swiss-Prot;Acc:Q94124])

HSP 1 Score: 207.608 bits (527), Expect = 8.280e-66
Identity = 102/195 (52.31%), Postives = 135/195 (69.23%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVIN--QTP--KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC L+SYTTN FP EY+ T+FD +  N+MVDG  INLSLWDTAGQ+ YDQ R   +  T VFLVCF++ +  ++ENV+  W  E+ ++CP  PIILVGTK DLR             +  V++ +G ++AK+I AVK++ECSA ++ GL QVF+EAIR  +   QTP  + KK  C++L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLLSYTTNAFPGEYILTVFDTYSTNVMVDGRPINLSLWDTAGQDDYDQFRHLSFPQTDVFLVCFALNNPASFENVRAKWYPEVSHHCPNTPIILVGTKADLREDRDTIERLRERRLQPVSHTQGYVMAKEIKAVKYLECSALTQIGLKQVFDEAIRTGLTPPQTPQTRAKKSNCTVL 195          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Celegans
Match: cdc-42 (Cell division control protein 42 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q05062])

HSP 1 Score: 201.83 bits (512), Expect = 1.180e-63
Identity = 103/198 (52.02%), Postives = 133/198 (67.17%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLR-------HLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+ IKCVVVGDG VGKTC LISYTTN+FP+EYVPT+FDN+   +M+ G    L L+DTAGQE YD+LR   Y  T VFLVCFSV+   ++ENV+  W  EI ++C K P +LVGT+VDLR        LA       ST+V       G  LAK++ AVK+VECSA +++GL  VF+EAI A ++   + KK KC++L
Sbjct:    1 MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVAPASFENVREKWVPEISHHCSKTPFLLVGTQVDLRDDPGMLEKLAKNKQKPVSTDV-------GEKLAKELKAVKYVECSALTQKGLKNVFDEAILAALDPPQQEKKKKCNIL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Celegans
Match: rho-1 (Ras-like GTP-binding protein rhoA [Source:UniProtKB/Swiss-Prot;Acc:Q22038])

HSP 1 Score: 175.637 bits (444), Expect = 1.730e-53
Identity = 82/181 (45.30%), Postives = 119/181 (65.75%), Query Frame = 1
Query:   76 MKAI--KCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQ 612
            M AI  K V+VGDG  GKTC LI ++ ++FP+ YVPT+F+N+ A++ VDG  + L+LWDTAGQE YD+LR   Y +T V L+CFS+    + EN+   W  E+R++CP +PIILVG K DLR                V  ++G  +A+QI A  ++ECSAK+++G+ +VFE+A +A + Q
Sbjct:    1 MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPDVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVRHFCPNVPIILVGNKRDLRSDPQTVRELAKMKQEPVKPEQGRAIAEQIGAFAYLECSAKTKDGIREVFEKATQAALQQ 181          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Fly
Match: Rac2 (gene:FBgn0014011 transcript:FBtr0076867)

HSP 1 Score: 227.254 bits (578), Expect = 1.515e-73
Identity = 109/194 (56.19%), Postives = 143/194 (73.71%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAV---INQTPKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   INL LWDTAGQE YD+LR   Y  T VFL+CFS+++  ++ENV+  W  E+R++CP +PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA +++GL  VF+EAIR+V   + + PKR   KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDAKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWFPEVRHHCPSVPIILVGTKLDLRDDKQTIEKLKDKKLTPITYPQGLAMAKEIAAVKYLECSALTQKGLKTVFDEAIRSVLCPVVRGPKRH--KCALL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Fly
Match: Rac2 (gene:FBgn0014011 transcript:FBtr0332800)

HSP 1 Score: 227.254 bits (578), Expect = 1.515e-73
Identity = 109/194 (56.19%), Postives = 143/194 (73.71%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAV---INQTPKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   INL LWDTAGQE YD+LR   Y  T VFL+CFS+++  ++ENV+  W  E+R++CP +PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA +++GL  VF+EAIR+V   + + PKR   KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDAKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWFPEVRHHCPSVPIILVGTKLDLRDDKQTIEKLKDKKLTPITYPQGLAMAKEIAAVKYLECSALTQKGLKTVFDEAIRSVLCPVVRGPKRH--KCALL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Fly
Match: Rac1 (gene:FBgn0010333 transcript:FBtr0072730)

HSP 1 Score: 226.483 bits (576), Expect = 3.726e-73
Identity = 109/193 (56.48%), Postives = 142/193 (73.58%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQT--PKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   INL LWDTAGQE YD+LR   Y  T VFL+CFS+++  ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA +++GL  VF+EAIR+V+     PK K+ KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDAKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWYPEVRHHCPSTPIILVGTKLDLRDDKNTIEKLRDKKLAPITYPQGLAMAKEIGAVKYLECSALTQKGLKTVFDEAIRSVLCPVLQPKSKR-KCALL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Fly
Match: Rac1 (gene:FBgn0010333 transcript:FBtr0331868)

HSP 1 Score: 226.483 bits (576), Expect = 3.726e-73
Identity = 109/193 (56.48%), Postives = 142/193 (73.58%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQT--PKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   INL LWDTAGQE YD+LR   Y  T VFL+CFS+++  ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA +++GL  VF+EAIR+V+     PK K+ KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDAKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWYPEVRHHCPSTPIILVGTKLDLRDDKNTIEKLRDKKLAPITYPQGLAMAKEIGAVKYLECSALTQKGLKTVFDEAIRSVLCPVLQPKSKR-KCALL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Fly
Match: Mtl (gene:FBgn0039532 transcript:FBtr0085192)

HSP 1 Score: 207.994 bits (528), Expect = 7.045e-66
Identity = 100/191 (52.36%), Postives = 134/191 (70.16%), Query Frame = 1
Query:   79 KAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            + IKCVVVGDG VGKTC LISYTT+ FP EYVPT+FDN+ A + VD I ++L LWDTAGQE YD+LR   Y  T VFL+C+SV   +++ENV   W  EI+++CP  PIILVGTK+DLR      +      ++ +  ++G  LA +I AVK++ECSA ++ GL  VFEEA+RAV+   P KR++ KC ++
Sbjct:    5 RPIKCVVVGDGTVGKTCMLISYTTDCFPGEYVPTVFDNYSAPMQVDTIQVSLGLWDTAGQEDYDRLRPLSYPQTDVFLICYSVASPSSFENVTSKWYPEIKHHCPDAPIILVGTKIDLREDRETLSGLAEQGLTPLKREQGQKLANKIRAVKYMECSALTQRGLKPVFEEAVRAVLRPEPLKRRQRKCLIM 195          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Zebrafish
Match: rac1b (Rac family small GTPase 1b [Source:ZFIN;Acc:ZDB-GENE-060312-45])

HSP 1 Score: 230.335 bits (586), Expect = 1.575e-74
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++C   PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KCSLL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCQTTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCSLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Zebrafish
Match: rac3b (Rac family small GTPase 3b [Source:ZFIN;Acc:ZDB-GENE-080220-21])

HSP 1 Score: 228.794 bits (582), Expect = 5.975e-74
Identity = 108/192 (56.25%), Postives = 140/192 (72.92%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPK-CSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             +S +TY +GL +A++I AVK++ECSA ++ GL  VF+EAIRAV+   P +K+ K C+L 
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIERLRDKKLSPITYPQGLAMAREIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRGKRCTLF 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Zebrafish
Match: rac2 (Rac family small GTPase 2 [Source:ZFIN;Acc:ZDB-GENE-040625-27])

HSP 1 Score: 227.639 bits (579), Expect = 1.407e-73
Identity = 109/192 (56.77%), Postives = 139/192 (72.40%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL LAK+I+AVK++ECSA ++ GL  VF+EAIRAV+   P K KK  C +L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPSTPIILVGTKLDLRDEKETIEKLKEKKLAPITYPQGLALAKEIDAVKYLECSALTQRGLKTVFDEAIRAVLCPQPTKVKKKGCVML 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Zebrafish
Match: rac2 (Rac family small GTPase 2 [Source:ZFIN;Acc:ZDB-GENE-040625-27])

HSP 1 Score: 227.639 bits (579), Expect = 1.407e-73
Identity = 109/192 (56.77%), Postives = 139/192 (72.40%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL LAK+I+AVK++ECSA ++ GL  VF+EAIRAV+   P K KK  C +L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPSTPIILVGTKLDLRDEKETIEKLKEKKLAPITYPQGLALAKEIDAVKYLECSALTQRGLKTVFDEAIRAVLCPQPTKVKKKGCVML 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Zebrafish
Match: rac3a (Rac family small GTPase 3a [Source:ZFIN;Acc:ZDB-GENE-040718-256])

HSP 1 Score: 227.254 bits (578), Expect = 2.367e-73
Identity = 106/192 (55.21%), Postives = 140/192 (72.92%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +A++I AVK++ECSA ++ GL  VF+EAIRAV+   P K++  KC++ 
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIERLRDKKLAPITYPQGLAMAREIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRGKKCTVF 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Xenopus
Match: rac1 (Rac family small GTPase 1 [Source:NCBI gene;Acc:448118])

HSP 1 Score: 231.491 bits (589), Expect = 6.956e-75
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Xenopus
Match: yju2 (YJU2 splicing factor homolog [Source:Xenbase;Acc:XB-GENE-5938025])

HSP 1 Score: 229.95 bits (585), Expect = 2.698e-74
Identity = 110/192 (57.29%), Postives = 138/192 (71.88%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRK-KPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   +YENV+  W  E+R++CP  PIILVGTK+DLR             +S ++Y +GL LAK+I AVK++ECSA ++ GL  VF+EAIRAV+   P R  K KC +L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWYPEVRHHCPSTPIILVGTKLDLRDDKETIEKLKEKKLSPISYPQGLALAKEIEAVKYLECSALTQRGLKTVFDEAIRAVLCPPPSRPNKAKCVVL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Xenopus
Match: nab2 (NGFI-A binding protein 2 (EGR1 binding protein 2) [Source:Xenbase;Acc:XB-GENE-957953])

HSP 1 Score: 227.254 bits (578), Expect = 3.127e-73
Identity = 107/192 (55.73%), Postives = 139/192 (72.40%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             +S +TY +GL +A++I +VK++ECSA ++ GL  VF+EAIRAV+   P KR   KC++ 
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIERLRDKRLSPITYPQGLAMAREIGSVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKRAGKKCTVF 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Xenopus
Match: rhogl.1 (ras homolog family member G [Source:NCBI gene;Acc:100489878])

HSP 1 Score: 221.09 bits (562), Expect = 8.467e-71
Identity = 104/191 (54.45%), Postives = 139/191 (72.77%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M++IKCVVVGDG VGKTC LI YTTN FP EY+PT+FDN+ A   VDG  ++L+LWDTAGQE YD+LR   Y  T+VF++CFS+   T+YENVK+ W  E+ ++CP +PI+LVGTK DLR+ A         N   +T  +G  LAKQI+AVK++ECSA +++G+ +VF EA+RAV+N TP + K  C +L
Sbjct:    1 MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQTSVDGRTVSLNLWDTAGQEEYDRLRTLSYPQTNVFIICFSIASPTSYENVKHKWYPEVGHHCPNVPILLVGTKKDLRNNADVMKKLKEQNQMPITNHQGGNLAKQIHAVKYMECSALNQDGIKEVFAEAVRAVLNPTPVKDKKSCFIL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Xenopus
Match: rhogl.1 (ras homolog family member G like gene 1 [Source:NCBI gene;Acc:100216282])

HSP 1 Score: 220.32 bits (560), Expect = 1.887e-70
Identity = 102/191 (53.40%), Postives = 138/191 (72.25%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+ IKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ A + VDG  ++L+LWDTAGQE YD+LR   Y  T+VF++CFS+   ++Y NV++ W  E+ ++CP +PI+LVGTK DLR+ +         +++  T Q+G  LAKQI AVK++ECSA  ++G+ QVFEEA+RAV+    K+   KC LL
Sbjct:    1 MQTIKCVVVGDGAVGKTCLLISYTTNAFPEEYIPTVFDNYSAQMTVDGRTVSLNLWDTAGQEEYDRLRTLSYPQTNVFIICFSIGSPSSYANVRHKWHPEVSHHCPNVPILLVGTKQDLRNDSETIKKLKEQSLAPTTNQQGSSLAKQIGAVKYMECSALHQQGVRQVFEEAVRAVLYPVTKKNPKKCVLL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Mouse
Match: Rac1 (Rac family small GTPase 1 [Source:MGI Symbol;Acc:MGI:97845])

HSP 1 Score: 231.491 bits (589), Expect = 5.268e-75
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Mouse
Match: Rac2 (Rac family small GTPase 2 [Source:MGI Symbol;Acc:MGI:97846])

HSP 1 Score: 229.95 bits (585), Expect = 2.594e-74
Identity = 109/192 (56.77%), Postives = 140/192 (72.92%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPK-CSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   +YENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL LAK I++VK++ECSA ++ GL  VF+EAIRAV+   P R++ + CSLL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWFPEVRHHCPSTPIILVGTKLDLRDDKDTIEKLKEKKLAPITYPQGLALAKDIDSVKYLECSALTQRGLKTVFDEAIRAVLCPQPTRQQKRPCSLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Mouse
Match: Rac3 (Rac family small GTPase 3 [Source:MGI Symbol;Acc:MGI:2180784])

HSP 1 Score: 219.935 bits (559), Expect = 1.834e-70
Identity = 100/177 (56.50%), Postives = 132/177 (74.58%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVI 606
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PI+LVGTK+DLR             ++ +TY +GL +A++I +VK++ECSA ++ GL  VF+EAIRAV+
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPHTPILLVGTKLDLRDDKDTIERLRDKKLAPITYPQGLAMAREIGSVKYLECSALTQRGLKTVFDEAIRAVL 177          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Mouse
Match: Rac1 (Rac family small GTPase 1 [Source:MGI Symbol;Acc:MGI:97845])

HSP 1 Score: 220.32 bits (560), Expect = 2.139e-70
Identity = 110/211 (52.13%), Postives = 141/211 (66.82%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNT-------------------SVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T                    VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTVGDTCGKDRPSRGKDKPIADVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 211          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Mouse
Match: Rhog (ras homolog family member G [Source:MGI Symbol;Acc:MGI:1928370])

HSP 1 Score: 218.394 bits (555), Expect = 6.232e-70
Identity = 101/191 (52.88%), Postives = 136/191 (71.20%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M++IKCVVVGDG VGKTC LI YTTN FP EY+PT+FDN+ A   VDG  +NL+LWDTAGQE YD+LR   Y  T+VF++CFS+    +YENV++ W  E+ ++CP +PI+LVGTK DLR              + +T Q+G  LAKQI+AV+++ECSA  ++G+ +VF EA+RAV+N TP ++   C LL
Sbjct:    1 MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQSAVDGRTVNLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCPDVPILLVGTKKDLRAQPDTLRRLKEQGQAPITPQQGQALAKQIHAVRYLECSALQQDGVKEVFAEAVRAVLNPTPIKRGRSCILL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. UniProt/SwissProt
Match: sp|Q6RUV5|RAC1_RAT (Ras-related C3 botulinum toxin substrate 1 OS=Rattus norvegicus OX=10116 GN=Rac1 PE=1 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 3.685e-74
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. UniProt/SwissProt
Match: sp|P63001|RAC1_MOUSE (Ras-related C3 botulinum toxin substrate 1 OS=Mus musculus OX=10090 GN=Rac1 PE=1 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 3.685e-74
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. UniProt/SwissProt
Match: sp|P63000|RAC1_HUMAN (Ras-related C3 botulinum toxin substrate 1 OS=Homo sapiens OX=9606 GN=RAC1 PE=1 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 3.685e-74
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. UniProt/SwissProt
Match: sp|P62999|RAC1_CANLF (Ras-related C3 botulinum toxin substrate 1 OS=Canis lupus familiaris OX=9615 GN=RAC1 PE=2 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 3.685e-74
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. UniProt/SwissProt
Match: sp|P62998|RAC1_BOVIN (Ras-related C3 botulinum toxin substrate 1 OS=Bos taurus OX=9913 GN=RAC1 PE=1 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 3.685e-74
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. TrEMBL
Match: A0A4U5U475 (Ras-related C3 botulinum toxin substrate 2 GX OS=Collichthys lucidus OX=240159 GN=D9C73_002644 PE=4 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 4.511e-74
Identity = 114/192 (59.38%), Postives = 142/192 (73.96%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVI-NQTPKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   +YENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL LAK+I+AVK++ECSA ++ GL  VF+EAIRAV+  QTPK KK  CSLL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWYPEVRHHCPSTPIILVGTKLDLRDEKDTIEKLKEKKLAPITYPQGLALAKEIDAVKYLECSALTQRGLKTVFDEAIRAVLCPQTPKVKKKGCSLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. TrEMBL
Match: A0A553P2W1 (Uncharacterized protein OS=Tigriopus californicus OX=6832 GN=TCAL_01129 PE=4 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 4.264e-73
Identity = 111/192 (57.81%), Postives = 144/192 (75.00%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRK-KPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  INL LWDTAGQE YD+LR   Y  T VFL+CFS+++  ++ENV+  W  E+R++CP +PIILVGTK+DLR      +      +S +TY +GL +AK + AVK++ECSA +++GL  VF+EAIRAV+   PK K + KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWYPEVRHHCPSVPIILVGTKLDLRDEKDTIDKLKEKRLSPITYPQGLAMAKDVGAVKYLECSALTQKGLKTVFDEAIRAVLCPAPKPKPRRKCNLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. TrEMBL
Match: A0A3Q3EYP8 (Rac family small GTPase 1 OS=Labrus bergylta OX=56723 GN=RAC1 (1 of many) PE=4 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 6.872e-73
Identity = 110/192 (57.29%), Postives = 143/192 (74.48%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W+ E+R++CP  PIILVGTK+DLR             +S + Y +GL +AK+IN+VK++ECSA ++ GL  VF+EAIRAV+   P K++K KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWSPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLSPIIYPQGLAMAKEINSVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCTLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. TrEMBL
Match: A0A084VDM0 (Rac gtpase OS=Anopheles sinensis OX=74873 GN=ZHAS_00003093 PE=4 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 8.814e-73
Identity = 111/191 (58.12%), Postives = 142/191 (74.35%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  INL LWDTAGQE YD+LR   Y  T VFL+CFS+++  ++ENV+  W  E+R++CP IPIILVGTK+DLR      +      +S +TY +GL +AK+I AVK++ECSA +++GL  VF+EAIRAV+      KK KC +L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWYPEVRHHCPNIPIILVGTKLDLRDDKNTVDKLRDKKLSPITYPQGLAMAKEIGAVKYLECSALTQKGLKTVFDEAIRAVLCPMIPPKKSKCKIL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. TrEMBL
Match: A0A182ISJ0 (Uncharacterized protein OS=Anopheles atroparvus OX=41427 PE=4 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 8.814e-73
Identity = 111/191 (58.12%), Postives = 142/191 (74.35%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  INL LWDTAGQE YD+LR   Y  T VFL+CFS+++  ++ENV+  W  E+R++CP IPIILVGTK+DLR      +      +S +TY +GL +AK+I AVK++ECSA +++GL  VF+EAIRAV+      KK KC +L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVNPASFENVRAKWYPEVRHHCPNIPIILVGTKLDLRDDKNTVDKLRDKKLSPITYPQGLAMAKEIGAVKYLECSALTQKGLKTVFDEAIRAVLCPMIPPKKSKCKIL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Cavefish
Match: rac1b (ras-related C3 botulinum toxin substrate 1-like [Source:NCBI gene;Acc:111192975])

HSP 1 Score: 231.876 bits (590), Expect = 3.441e-75
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K +K KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDQDTIEKLKERKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKSRKRKCTLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Cavefish
Match: rac1a (ras-related C3 botulinum toxin substrate 1 [Source:NCBI gene;Acc:103034053])

HSP 1 Score: 231.491 bits (589), Expect = 4.137e-75
Identity = 110/192 (57.29%), Postives = 141/192 (73.44%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+   P K++K KC LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Cavefish
Match: rac1b (ras-related C3 botulinum toxin substrate 1-like [Source:NCBI gene;Acc:111192975])

HSP 1 Score: 229.18 bits (583), Expect = 3.779e-74
Identity = 109/192 (56.77%), Postives = 140/192 (72.92%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++  L  VF+EAIRAV+   P K +K KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDQDTIEKLKERKLTPITYPQGLAMAKEIGAVKYLECSALTQRDLKTVFDEAIRAVLCPPPVKSRKRKCTLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Cavefish
Match: rac1b (ras-related C3 botulinum toxin substrate 1-like [Source:NCBI gene;Acc:111192975])

HSP 1 Score: 229.18 bits (583), Expect = 3.779e-74
Identity = 109/192 (56.77%), Postives = 140/192 (72.92%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++  L  VF+EAIRAV+   P K +K KC+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDQDTIEKLKERKLTPITYPQGLAMAKEIGAVKYLECSALTQRDLKTVFDEAIRAVLCPPPVKSRKRKCTLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Cavefish
Match: rac3b (ras-related C3 botulinum toxin substrate 3-like [Source:NCBI gene;Acc:111192386])

HSP 1 Score: 228.794 bits (582), Expect = 5.064e-74
Identity = 108/192 (56.25%), Postives = 140/192 (72.92%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPK-CSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             +S +TY +GL +A++I AVK++ECSA ++ GL  VF+EAIRAV+   P +K+ K C+L 
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIERLRDKKLSPITYPQGLAMAREIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRGKRCTLF 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007368.1 (pep scaffold:Pmarinus_7.0:GL476419:214196:224439:-1 gene:ENSPMAG00000006646.1 transcript:ENSPMAT00000007368.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 224.557 bits (571), Expect = 4.977e-73
Identity = 107/193 (55.44%), Postives = 139/193 (72.02%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPK--RKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LIS++ N FP EY+PT+FDN+  NLMVDG  +NL LWDTAGQE YD+LR   Y  T +FL+CFSV+  ++Y+NV+  W KE+R++CP+ PIILVGTK D R            ++  VT  +GL LAK+I A+K++ECSA ++ GL  VF+E +RAVI+  PK  RKK  C+LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCMLISFSNNTFPGEYIPTVFDNYAVNLMVDGKYVNLGLWDTAGQEDYDRLRPLSYPQTDIFLICFSVVSPSSYDNVRVKWFKEVRHHCPETPIILVGTKQDQREDKDILKHLEEKSMQPVTNAQGLSLAKEIGAIKYLECSALTQRGLKNVFDETVRAVISPEPKIERKKKSCALL 193          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009835.1 (pep scaffold:Pmarinus_7.0:GL479385:19931:31465:1 gene:ENSPMAG00000008880.1 transcript:ENSPMAT00000009835.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 223.016 bits (567), Expect = 1.767e-72
Identity = 103/177 (58.19%), Postives = 132/177 (74.58%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVI 606
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL +AK+I AVK++ECSA ++ GL  VF+EAIRAV+
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTVEKLREKKLAPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVL 177          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004439.1 (pep scaffold:Pmarinus_7.0:GL476335:1598569:1600956:1 gene:ENSPMAG00000004041.1 transcript:ENSPMAT00000004439.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 179.874 bits (455), Expect = 1.286e-55
Identity = 89/196 (45.41%), Postives = 126/196 (64.29%), Query Frame = 1
Query:   76 MKAI--KCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNS---TNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M AI  K V+VGDG  GKTC LI ++ ++FP  YVPT+F+N+ A++ VDG  + L+LWDTAGQE YD+LR   Y +T V L+CFS+    + EN+   W  E++++CP +PIILVG K DLR+    DNT           V  +EG  +A +INA  ++ECSAK+++G+ +VFE A RA +    ++K   C LL
Sbjct:    1 MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRN---DDNTKRELAKMKQEPVRVEEGRDMANRINAFGYMECSAKTKDGVREVFEMATRAALQVRKRKKTGGCRLL 193          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Sea Lamprey
Match: rhoj (ras homolog family member J [Source:ZFIN;Acc:ZDB-GENE-040724-272])

HSP 1 Score: 178.718 bits (452), Expect = 6.290e-55
Identity = 84/185 (45.41%), Postives = 121/185 (65.41%), Query Frame = 1
Query:   85 IKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKC 639
            +KCVVVGDG VGKTC ++SY  + FP +YVPT+FD++  N+ V G    L L+DTAGQE Y QLR   Y NT +F++CFSV++  +Y NVK  W  E+R  CP +P +LVGT++DLR                +T+Q+G+ LAKQI A  ++ECSA +++GL  VF+EAI  ++ +   ++  +C
Sbjct:   21 LKCVVVGDGAVGKTCLIMSYANDAFPEQYVPTVFDHYAVNVKVGGSCYLLGLYDTAGQEDYHQLRPLSYPNTDMFMICFSVVNPASYHNVKEEWVPELRACCPDVPYLLVGTQIDLRDATKTLMRLKELKEKPMTHQQGVKLAKQIGAHCYLECSALTQKGLKAVFDEAILTILQRKKCKRSTRC 205          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001838.1 (pep scaffold:Pmarinus_7.0:GL478831:173097:182385:-1 gene:ENSPMAG00000001664.1 transcript:ENSPMAT00000001838.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 175.252 bits (443), Expect = 9.873e-54
Identity = 84/184 (45.65%), Postives = 119/184 (64.67%), Query Frame = 1
Query:   76 MKAI--KCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPK 621
            M AI  K V+VGDG  GKTC LI ++ ++FP  YVPT+F+N+ A++ VDG  + L+LWDTAGQE YD+LR   Y +T V L+CFS+    + EN+   W  E++++CP +PIILVG K DLR+               V  +EG  +A +INA  ++ECSAK+++G+ +VFE A RA +  T K
Sbjct:    1 MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDESTRRELAKMKQEPVKSEEGRDMANRINAFGYMECSAKTKDGVREVFEMATRAALQLTAK 184          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Yeast
Match: CDC42 (Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; mutants have defects in the organization of actin and septins; human homolog CDC42 can complement yeast cdc42 mutant [Source:SGD;Acc:S000004219])

HSP 1 Score: 196.438 bits (498), Expect = 2.338e-62
Identity = 92/191 (48.17%), Postives = 130/191 (68.06%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+ +KCVVVGDG VGKTC LISYTTN+FP +YVPT+FDN+   +M+      L L+DTAGQE YD+LR   Y +T VFLVCFSV+   ++ENVK  W  E+ ++CP +P ++VGT++DLR   +         +  +T ++G  LA+++ AVK+VECSA ++ GL  VF+EAI A +     +K  KC++L
Sbjct:    1 MQTLKCVVVGDGAVGKTCLLISYTTNQFPADYVPTVFDNYAVTVMIGDEPYTLGLFDTAGQEDYDRLRPLSYPSTDVFLVCFSVISPPSFENVKEKWFPEVHHHCPGVPCLVVGTQIDLRDDKVIIEKLQRQRLRPITSEQGSRLARELKAVKYVECSALTQRGLKNVFDEAIVAALEPPVIKKSKKCAIL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Yeast
Match: RHO1 (GTP-binding protein of the rho subfamily of Ras-like proteins; involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) [Source:SGD;Acc:S000006369])

HSP 1 Score: 163.31 bits (412), Expect = 3.388e-49
Identity = 81/171 (47.37%), Postives = 110/171 (64.33%), Query Frame = 1
Query:   88 KCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRA 600
            K V+VGDG  GKTC LI ++  +FP  YVPT+F+N+ A++ VDG  + L+LWDTAGQE YD+LR   Y +++V L+CFS+    + ENV+  W  E+ ++C  +PIILVG KVDLR+               VT QEG  +A QI A  + ECSAK+  G+ +VFE A RA
Sbjct:   12 KLVIVGDGACGKTCLLIVFSKGQFPEVYVPTVFENYVADVEVDGRRVELALWDTAGQEDYDRLRPLSYPDSNVVLICFSIDLPDSLENVQEKWIAEVLHFCQGVPIILVGCKVDLRNDPQTIEQLRQEGQQPVTSQEGQSVADQIGATGYYECSAKTGYGVREVFEAATRA 182          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Yeast
Match: RHO4 (Non-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi [Source:SGD;Acc:S000001763])

HSP 1 Score: 154.451 bits (389), Expect = 6.630e-45
Identity = 77/178 (43.26%), Postives = 111/178 (62.36%), Query Frame = 1
Query:   85 IKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLM-VDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQT 615
            +K VVVGDG VGKTC LISY    FP +Y+PT+F+N+  N+   +G  I L+LWDTAGQE Y +LR   Y N  V +VC+SV  +T+ +NV+  W  E++++CP  PI+LVG K DL      DN S+      V       LAK++ A   ++CSA+ +E + +VFE AI  +++ +
Sbjct:   73 LKIVVVGDGAVGKTCLLISYVQGTFPTDYIPTIFENYVTNIEGPNGQIIELALWDTAGQEEYSRLRPLSYTNADVLMVCYSVGSKTSLKNVEDLWFPEVKHFCPSTPIMLVGLKSDLYE---ADNLSD-----LVEPSSAESLAKRLGAFAHIQCSARLKENIDEVFETAIHTLLSDS 242          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Yeast
Match: RHO5 (Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; RAC1 ortholog; regulated by phosphorylation and ubiquitination; likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity [Source:SGD;Acc:S000005124])

HSP 1 Score: 152.14 bits (383), Expect = 1.739e-43
Identity = 85/233 (36.48%), Postives = 121/233 (51.93%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMV---------------DGIGI---------------NLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYC-----------PKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVI--NQTPKRKKPKCSL 645
            M++IKCV++GDG VGKT  LISYTTN FP +YVPT+FDN+   + +               D  G                 ++LWDTAGQE YD+LR  CY  T +FL+CFSV +  ++ NV   W  E++               K PI+LVGTK DLR            N  YV+ +E   L ++   + + ECSA ++ G+ +VFE+A+R  I   ++P +K    +L
Sbjct:    1 MRSIKCVIIGDGAVGKTSLLISYTTNSFPTDYVPTVFDNYSTTIAIPNGTASSPLELDNGNDKRGSLSSASSSPSTDRKLYKINLWDTAGQEDYDRLRPLCYPQTDIFLICFSVSEHASFANVTEKWLPELKQTSNIEGTSLYTKLGKYPILLVGTKSDLRDDPATQKKLQEANSDYVSQEEIDELVQRCGFMGYTECSAATQAGVREVFEQAVRYAIYEPESPNQKSANHTL 233          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Yeast
Match: RHO3 (Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p [Source:SGD;Acc:S000001380])

HSP 1 Score: 148.288 bits (373), Expect = 3.655e-43
Identity = 79/200 (39.50%), Postives = 117/200 (58.50%), Query Frame = 1
Query:   88 KCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRH-----LAI---------------GDN---TSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP 618
            K V++GDG  GKT  L  +T   FP  Y PT+F+N+  ++ VD   I LSLWDTAGQE +D+LR   Y +T   ++CFS+  R + ENV+  W  EI ++C  + ++LV  K DLR+      AI               G+N   TSN  N+  ++Y+EGL +AK+I A++++ECSAK  +G+++ F EA R  +   P
Sbjct:   18 KIVILGDGACGKTSLLNVFTRGYFPEVYEPTVFENYIHDIFVDSKHITLSLWDTAGQEEFDRLRSLSYSDTQCIMLCFSIDSRDSLENVQNKWVGEITDHCEGVKLVLVALKCDLRNNENESNAITPNNIQQDNSVSNDNGNNINSTSNGKNL--ISYEEGLAMAKKIGALRYLECSAKLNKGVNEAFTEAARVALTAGP 215          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Nematostella
Match: EDO38536 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCK6])

HSP 1 Score: 226.868 bits (577), Expect = 1.026e-73
Identity = 109/194 (56.19%), Postives = 139/194 (71.65%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPK---CSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  INL LWDTAGQE YD+LR   Y  T VFL+CFS++   +YENV+  W  E+ ++CP  PI+LVGTK+DLR      +      +  + Y +GL +AK+I AVK++ECSA +++GL  VF+EAIRAV+  T   KKP+   C LL
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLISPASYENVRAKWYPEVSHHCPNTPIVLVGTKLDLRDDKETIDKLKEKKLGPIGYTQGLQMAKEIGAVKYLECSALTQKGLKTVFDEAIRAVLCPTKPAKKPRNKGCKLL 194          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Nematostella
Match: EDO35019 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMN7])

HSP 1 Score: 213.001 bits (541), Expect = 2.279e-68
Identity = 100/178 (56.18%), Postives = 128/178 (71.91%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVIN 609
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  INL LWDTAGQE YD+LR   Y  T VFL+CFSV+   ++ENV+  W  E+ ++CP  PIILVGTK DLR             ++ ++   GL +AK+I A K++ECSA +++GL  VF++AIRAV+ 
Sbjct:    1 MQAIKCVVVGDGAVGKTCMLISYTTNAFPGEYIPTVFDNYSANVMVDGRPINLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSVISPASFENVRAKWYPEVSHHCPGTPIILVGTKTDLREDKEMIEKLKEKKLNPLSTSHGLQMAKEIKAAKYLECSALTQKGLKTVFDDAIRAVLE 178          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Nematostella
Match: EDO37472 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFJ1])

HSP 1 Score: 204.527 bits (519), Expect = 5.356e-65
Identity = 99/191 (51.83%), Postives = 129/191 (67.54%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+ IKCVVVGDG VGKTC LISYTTN+FP+EYVPT+FDN+   +M+ G    L L+DTAGQE YD+LR   Y  T VFLVCFSV+  +++ENVK  W  EI ++CPK P +LVGT+VDLR         +      +  +    LA+++ AVK+VECSA +++GL  VF+EAI A +     +KK KC LL
Sbjct:    1 MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQVDLRDDQGTIEKLSKNKQKPIAVEAAEKLARELRAVKYVECSALTQKGLKNVFDEAILAALEPPEPQKKKKCRLL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Nematostella
Match: EDO39465 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9Y5])

HSP 1 Score: 173.711 bits (439), Expect = 4.812e-53
Identity = 90/193 (46.63%), Postives = 126/193 (65.28%), Query Frame = 1
Query:   76 MKAI--KCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M AI  K V+VGDG  GKTC LI ++ ++FP  YVPT+F+N+ A++ VDG  + L+LWDTAGQE YD+LR   Y +T V L+CFS+    + EN+   W  E++++CP +PIILVG K DLR                V  +EG  +A +I+A  ++ECSAK+++G+ +VFE A RA + QT K+KK KC LL
Sbjct:    1 MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRMDENTKRELQKMKQEPVKIEEGRAMADKISAYAYLECSAKTKDGVREVFETATRAAL-QTKKKKKGKCVLL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Nematostella
Match: EDO36554 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI96])

HSP 1 Score: 144.821 bits (364), Expect = 7.496e-42
Identity = 72/189 (38.10%), Postives = 110/189 (58.20%), Query Frame = 1
Query:   85 IKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLM-VDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            +K  +VGDG VGKT   I+  TN FP EY+PT +D    +   VDGI     L+D+AG E +D+LR   Y NT +FLVCF +  R ++ENV   W  E+ ++ P +P +LVG K DLR      +  +S ++ +V+Y+EG   A+    ++++E SA   E + +V   +IR   +  PK+ K KC ++
Sbjct:    7 LKLYIVGDGAVGKTALFITAATNTFPTEYIPTAYDASEQDFFNVDGIKYQAVLFDSAGGEDFDRLRPLGYPNTDIFLVCFDIAHRASFENVALRWLPELDHFSPGVPRVLVGNKKDLRP---KQDQEDSHSIDFVSYEEGKEAAQNFK-MQYIENSALLNENVKEVITTSIRIARDNLPKKGKRKCHVM 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Medaka
Match: ENSORLT00000038381.1 (ras-related C3 botulinum toxin substrate 1 [Source:NCBI gene;Acc:101175028])

HSP 1 Score: 231.106 bits (588), Expect = 6.344e-75
Identity = 110/192 (57.29%), Postives = 140/192 (72.92%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKP-KCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             +S + Y +GL +AK+I+AVK++ECSA ++ GL  VF+EAIRAV+   P RKK  KC +L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPVTPIILVGTKLDLRDDKDTIEKLKEKKLSPIIYPQGLAMAKEISAVKYLECSALTQRGLKTVFDEAIRAVLCPPPDRKKKRKCRIL 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Medaka
Match: ENSORLT00000041989.1 (Rac family small GTPase 2 [Source:NCBI gene;Acc:101163549])

HSP 1 Score: 229.565 bits (584), Expect = 2.114e-74
Identity = 110/192 (57.29%), Postives = 139/192 (72.40%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTP-KRKKPKCSLL 648
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   +YENV+  W  E+R++CP  PIILVGTK+DLR             ++ +TY +GL LAK+I+AVK++ECSA ++ GL  VF+EAIRAV+   P K KK  C +L
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASYENVRAKWYPEVRHHCPSTPIILVGTKLDLRDEKDTIEKLKEKKLAPITYPQGLALAKEIDAVKYLECSALTQRGLKTVFDEAIRAVLCPQPAKVKKANCLML 192          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Medaka
Match: RAC1 (ras-related C3 botulinum toxin substrate 1 [Source:NCBI gene;Acc:101159016])

HSP 1 Score: 224.557 bits (571), Expect = 2.518e-72
Identity = 103/177 (58.19%), Postives = 132/177 (74.58%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVI 606
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             +S +TY +GL +AK+I +VK++ECSA ++ GL  VF+EAIRAV+
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKETTEKLKEKKLSPITYPQGLAMAKEIGSVKYLECSALTQRGLKTVFDEAIRAVL 177          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Medaka
Match: RAC1 (ras-related C3 botulinum toxin substrate 1 [Source:NCBI gene;Acc:101159016])

HSP 1 Score: 224.557 bits (571), Expect = 2.518e-72
Identity = 103/177 (58.19%), Postives = 132/177 (74.58%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVI 606
            M+AIKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVDG  +NL LWDTAGQE YD+LR   Y  T VFL+CFS++   ++ENV+  W  E+R++CP  PIILVGTK+DLR             +S +TY +GL +AK+I +VK++ECSA ++ GL  VF+EAIRAV+
Sbjct:    1 MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKETTEKLKEKKLSPITYPQGLAMAKEIGSVKYLECSALTQRGLKTVFDEAIRAVL 177          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Medaka
Match: rhogb (rho-related GTP-binding protein RhoG [Source:NCBI gene;Acc:101162944])

HSP 1 Score: 208.379 bits (529), Expect = 3.946e-66
Identity = 95/191 (49.74%), Postives = 134/191 (70.16%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+ +KCVVVGDG VGKTC LISYTT  FP EY+PT+FDN+ + + VDG  I+L+LWDTAGQE YD+LR   Y  T+VF++CFS+    ++ENVK+ W  E+ ++CP +PI+LVGTK DLR+           N + +++ +G  LA+QI A++++ECSA +++G+  VF EA+RA +N  P   K  C LL
Sbjct:    1 MQTVKCVVVGDGAVGKTCLLISYTTGAFPKEYIPTVFDNYSSQVTVDGRVISLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSISSPASFENVKHKWHPEVSHHCPGVPILLVGTKSDLRNNEELVKKLKEQNQAPISHSQGTSLARQIQALRYLECSALNQDGIKDVFVEAVRAYLNPQPTVNKRPCVLL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Planmine SMEST
Match: SMESG000005013.1 (SMESG000005013.1)

HSP 1 Score: 398.282 bits (1022), Expect = 8.271e-141
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL
Sbjct:    1 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 191          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Planmine SMEST
Match: SMESG000024961.1 (SMESG000024961.1)

HSP 1 Score: 230.72 bits (587), Expect = 6.140e-75
Identity = 106/185 (57.30%), Postives = 137/185 (74.05%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKK 630
            M+ IKCVVVGDG VGKTC LISYTTN FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VFL+CFS+++ ++YENV+  W  EI ++CP  PIILVGTK+DLR   +          + ++Y +GL+LAK+I AVK++ECSA +++GL  VF+EAIRAV+   P RKK
Sbjct:    1 MQGIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDNKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLINSSSYENVRAKWYPEITHHCPNTPIILVGTKLDLRENHMNGEAGKDRRNTPISYPQGLVLAKEIGAVKYLECSALTQKGLKSVFDEAIRAVLCPKPPRKK 185          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Planmine SMEST
Match: SMESG000005010.1 (SMESG000005010.1)

HSP 1 Score: 226.483 bits (576), Expect = 3.547e-73
Identity = 111/195 (56.92%), Postives = 144/195 (73.85%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLR-HLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPK---CSLL 648
            M++IKCVVVGDG VGKTC LISYTT+ FP  +VPT+FDN  AN+M++G  INL LWDTAGQE YD+LR   Y  T VFL+CFS++++ ++ NVK  W KE+ ++  + PIILVGTK+DLR   A+ +N  N    S VTYQ+GL L ++I AVK++ECSA+++  L QVFEEAIR VIN+ PK   P+   CSLL
Sbjct:    1 MRSIKCVVVGDGAVGKTCILISYTTDAFPTTHVPTVFDNMSANVMIEGTPINLGLWDTAGQEEYDRLRPLSYPETDVFLICFSLVNKDSFANVKEKWYKEVSHFSKETPIILVGTKLDLREERALNENDKNQIRSSPVTYQQGLALCQEIGAVKYIECSARTQVNLRQVFEEAIRVVINK-PKPSNPRFKFCSLL 194          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Planmine SMEST
Match: SMESG000024968.1 (SMESG000024968.1)

HSP 1 Score: 221.861 bits (564), Expect = 1.638e-71
Identity = 103/191 (53.93%), Postives = 139/191 (72.77%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL 648
            M+ IKCVVVGDG VGKTC LISYTT+ FP EY+PT+FDN+ AN+MVD   +NL LWDTAGQE YD+LR   Y  T VF++CFS+++ ++YENV+  W  E+ N+CP  PIILVGTK+DLR     D +  +    +++Y +GLIL K+I A K++ECSA ++ GL  VF+E+IR V+    K+ K +CS+L
Sbjct:    1 MQGIKCVVVGDGAVGKTCLLISYTTSAFPGEYIPTVFDNYSANIMVDNKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFIICFSLVNPSSYENVRAKWYPEVNNHCPNTPIILVGTKLDLR-----DESQTNKTGGHISYPQGLILMKEIGAKKYLECSALTQTGLKTVFDESIRVVLCPKQKQNKKRCSIL 186          
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Planmine SMEST
Match: SMESG000005012.1 (SMESG000005012.1)

HSP 1 Score: 203.371 bits (516), Expect = 2.755e-64
Identity = 95/179 (53.07%), Postives = 131/179 (73.18%), Query Frame = 1
Query:   76 MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIGINLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKEIRNYCPKIPIILVGTKVDLR-HLAIGDNTSNSTNVSYVTYQEGLILAKQINAVKFVECSAKSREGLSQVFEEAIRAVIN 609
            M++IKCVV+GDG VGKTC L+S+TT+ F +  +PT+FDN  AN+M+DG  INL LWDTAGQ+ +D +RV  Y  T VFL+CFS++++ ++ NVK  W KE+ +Y    PIILVGTK+DLR +    ++       S VTYQ+GL L ++I AVK++ECSAK++E L  VFEEA+R V+N
Sbjct:    1 MRSIKCVVIGDGAVGKTCILVSFTTDVFSSSPIPTVFDNMSANMMIDGKPINLGLWDTAGQDDFDGIRVVSYPQTDVFLICFSLVNKVSFVNVKEKWFKEVSHYSENTPIILVGTKLDLRDNKDFDEHDKGQVFSSPVTYQQGLALCQEIGAVKYIECSAKTQENLKMVFEEAVRIVLN 179          
The following BLAST results are available for this feature:
BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RAC17.674e-7557.29Rac family small GTPase 1 [Source:HGNC Symbol;Acc:... [more]
RAC21.604e-7456.77Rac family small GTPase 2 [Source:HGNC Symbol;Acc:... [more]
RAC32.671e-7056.50Rac family small GTPase 3 [Source:HGNC Symbol;Acc:... [more]
RAC12.858e-7052.13Rac family small GTPase 1 [Source:HGNC Symbol;Acc:... [more]
RHOG9.077e-7052.88ras homolog family member G [Source:HGNC Symbol;Ac... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ced-103.031e-7356.02Ras-related protein ced-10 [Source:UniProtKB/Swis... [more]
mig-28.892e-7049.74Rac-like GTPase [Source:UniProtKB/TrEMBL;Acc:G5EB... [more]
rac-28.280e-6652.31Ras-related protein rac-2 [Source:UniProtKB/Swiss... [more]
cdc-421.180e-6352.02Cell division control protein 42 homolog [Source:... [more]
rho-11.730e-5345.30Ras-like GTP-binding protein rhoA [Source:UniProt... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rac21.515e-7356.19gene:FBgn0014011 transcript:FBtr0076867[more]
Rac21.515e-7356.19gene:FBgn0014011 transcript:FBtr0332800[more]
Rac13.726e-7356.48gene:FBgn0010333 transcript:FBtr0072730[more]
Rac13.726e-7356.48gene:FBgn0010333 transcript:FBtr0331868[more]
Mtl7.045e-6652.36gene:FBgn0039532 transcript:FBtr0085192[more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rac1b1.575e-7457.29Rac family small GTPase 1b [Source:ZFIN;Acc:ZDB-GE... [more]
rac3b5.975e-7456.25Rac family small GTPase 3b [Source:ZFIN;Acc:ZDB-GE... [more]
rac21.407e-7356.77Rac family small GTPase 2 [Source:ZFIN;Acc:ZDB-GEN... [more]
rac21.407e-7356.77Rac family small GTPase 2 [Source:ZFIN;Acc:ZDB-GEN... [more]
rac3a2.367e-7355.21Rac family small GTPase 3a [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rac16.956e-7557.29Rac family small GTPase 1 [Source:NCBI gene;Acc:44... [more]
yju22.698e-7457.29YJU2 splicing factor homolog [Source:Xenbase;Acc:X... [more]
nab23.127e-7355.73NGFI-A binding protein 2 (EGR1 binding protein 2) ... [more]
rhogl.18.467e-7154.45ras homolog family member G [Source:NCBI gene;Acc:... [more]
rhogl.11.887e-7053.40ras homolog family member G like gene 1 [Source:NC... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rac15.268e-7557.29Rac family small GTPase 1 [Source:MGI Symbol;Acc:M... [more]
Rac22.594e-7456.77Rac family small GTPase 2 [Source:MGI Symbol;Acc:M... [more]
Rac31.834e-7056.50Rac family small GTPase 3 [Source:MGI Symbol;Acc:M... [more]
Rac12.139e-7052.13Rac family small GTPase 1 [Source:MGI Symbol;Acc:M... [more]
Rhog6.232e-7052.88ras homolog family member G [Source:MGI Symbol;Acc... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6RUV5|RAC1_RAT3.685e-7457.29Ras-related C3 botulinum toxin substrate 1 OS=Ratt... [more]
sp|P63001|RAC1_MOUSE3.685e-7457.29Ras-related C3 botulinum toxin substrate 1 OS=Mus ... [more]
sp|P63000|RAC1_HUMAN3.685e-7457.29Ras-related C3 botulinum toxin substrate 1 OS=Homo... [more]
sp|P62999|RAC1_CANLF3.685e-7457.29Ras-related C3 botulinum toxin substrate 1 OS=Cani... [more]
sp|P62998|RAC1_BOVIN3.685e-7457.29Ras-related C3 botulinum toxin substrate 1 OS=Bos ... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4U5U4754.511e-7459.38Ras-related C3 botulinum toxin substrate 2 GX OS=C... [more]
A0A553P2W14.264e-7357.81Uncharacterized protein OS=Tigriopus californicus ... [more]
A0A3Q3EYP86.872e-7357.29Rac family small GTPase 1 OS=Labrus bergylta OX=56... [more]
A0A084VDM08.814e-7358.12Rac gtpase OS=Anopheles sinensis OX=74873 GN=ZHAS_... [more]
A0A182ISJ08.814e-7358.12Uncharacterized protein OS=Anopheles atroparvus OX... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rac1b3.441e-7557.29ras-related C3 botulinum toxin substrate 1-like [S... [more]
rac1a4.137e-7557.29ras-related C3 botulinum toxin substrate 1 [Source... [more]
rac1b3.779e-7456.77ras-related C3 botulinum toxin substrate 1-like [S... [more]
rac1b3.779e-7456.77ras-related C3 botulinum toxin substrate 1-like [S... [more]
rac3b5.064e-7456.25ras-related C3 botulinum toxin substrate 3-like [S... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000007368.14.977e-7355.44pep scaffold:Pmarinus_7.0:GL476419:214196:224439:-... [more]
ENSPMAT00000009835.11.767e-7258.19pep scaffold:Pmarinus_7.0:GL479385:19931:31465:1 g... [more]
ENSPMAT00000004439.11.286e-5545.41pep scaffold:Pmarinus_7.0:GL476335:1598569:1600956... [more]
rhoj6.290e-5545.41ras homolog family member J [Source:ZFIN;Acc:ZDB-G... [more]
ENSPMAT00000001838.19.873e-5445.65pep scaffold:Pmarinus_7.0:GL478831:173097:182385:-... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
CDC422.338e-6248.17Small rho-like GTPase; essential for establishment... [more]
RHO13.388e-4947.37GTP-binding protein of the rho subfamily of Ras-li... [more]
RHO46.630e-4543.26Non-essential small GTPase; member of the Rho/Rac ... [more]
RHO51.739e-4336.48Non-essential small GTPase of the Rho/Rac family o... [more]
RHO33.655e-4339.50Non-essential small GTPase of the Rho/Rac family o... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO385361.026e-7356.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO350192.279e-6856.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO374725.356e-6551.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO394654.812e-5346.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO365547.496e-4238.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000038381.16.344e-7557.29ras-related C3 botulinum toxin substrate 1 [Source... [more]
ENSORLT00000041989.12.114e-7457.29Rac family small GTPase 2 [Source:NCBI gene;Acc:10... [more]
RAC12.518e-7258.19ras-related C3 botulinum toxin substrate 1 [Source... [more]
RAC12.518e-7258.19ras-related C3 botulinum toxin substrate 1 [Source... [more]
rhogb3.946e-6649.74rho-related GTP-binding protein RhoG [Source:NCBI ... [more]
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BLAST of Ras-like C3 botulinum toxin substrate 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005013.18.271e-141100.00SMESG000005013.1[more]
SMESG000024961.16.140e-7557.30SMESG000024961.1[more]
SMESG000005010.13.547e-7356.92SMESG000005010.1[more]
SMESG000024968.11.638e-7153.93SMESG000024968.1[more]
SMESG000005012.12.755e-6453.07SMESG000005012.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003600 ID=SMED30003600|Name=Ras-like C3 botulinum toxin substrate 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1113bp
GTTTAAATTAAGTCTACATATCGCATATTTCTAAAGCACAAAATTCAAAA
AAACTTTAAATTTCTAAAAAGTCATATGAAAGCCATTAAATGTGTTGTGG
TTGGGGATGGTCAAGTTGGAAAAACATGCACATTAATCAGCTATACAACA
AACAGATTCCCCAATGAATATGTTCCAACAATGTTTGATAATTGGTGTGC
TAACTTAATGGTTGATGGAATTGGAATTAATCTCAGTCTTTGGGATACTG
CAGGACAGGAAACATATGATCAGCTTAGAGTTACGTGTTATCAAAATACA
AGCGTTTTTCTCGTATGTTTCTCGGTAATGGATAGAACAACCTATGAAAA
TGTGAAATACACTTGGGCTAAGGAGATTAGAAATTATTGTCCCAAAATAC
CAATAATACTTGTCGGAACCAAAGTAGATTTGAGACACTTAGCTATCGGG
GATAATACTTCAAATAGTACCAATGTATCGTATGTGACGTATCAAGAAGG
TCTGATTTTAGCCAAACAAATAAATGCTGTCAAATTCGTGGAATGTTCAG
CGAAGTCACGTGAAGGCCTAAGCCAGGTATTTGAGGAAGCGATCCGGGCT
GTAATCAATCAGACACCAAAAAGGAAAAAGCCCAAGTGCTCACTGTTGTA
GATTGAAATCAAATTCTACAAAATCTGCACTTGATCCAATATTCAGATCA
CTGAATCAATTGTGATGATGCTCAATTTTTGGTGCATAATTTATTATTAT
TCGTGCATTTTACATTTGATTTTAGCGACAAAAAAATTTTTGATAAAAAT
CTTAACTGAACTGTGTGACATTCTGAAACTCGATGCAGTGTTCAACTGAT
GCTGAATTATTATTAATATTATTATTAATATTATTTTAATATATTTAATT
CAATTTGAAATTAATTTTGAACTTTGATTTTACACATATCAAATATTTTA
TTGAAGATATCGATCTTTGTTTTATCAATTTATGTGCAACGCTGATGTAC
TCGATCATTATTAATTAGTAATCTACAATCGATATAATAATTAATTTATT
AATATTGATTTAATTGATGTTCCGTGATTGGCTGCGCTTACTTTTACTTA
CATTTTTTTACCG
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protein sequence of SMED30003600-orf-1

>SMED30003600-orf-1 ID=SMED30003600-orf-1|Name=SMED30003600-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=192bp
MKAIKCVVVGDGQVGKTCTLISYTTNRFPNEYVPTMFDNWCANLMVDGIG
INLSLWDTAGQETYDQLRVTCYQNTSVFLVCFSVMDRTTYENVKYTWAKE
IRNYCPKIPIILVGTKVDLRHLAIGDNTSNSTNVSYVTYQEGLILAKQIN
AVKFVECSAKSREGLSQVFEEAIRAVINQTPKRKKPKCSLL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR005225Small_GTP-bd_dom
IPR003578Small_GTPase_Rho
IPR027417P-loop_NTPase
IPR001806Small_GTPase
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0005515protein binding
GO:0000166nucleotide binding
GO:0003924GTPase activity
GO:0017137Rab GTPase binding
GO:0019899enzyme binding
GO:0019901protein kinase binding
GO:0030742GTP-dependent protein binding
GO:0031996thioesterase binding
GO:0042826histone deacetylase binding
GO:0051022Rho GDP-dissociation inhibitor binding
GO:0051117ATPase binding
Vocabulary: biological process
TermDefinition
GO:0007264small GTPase mediated signal transduction
GO:0001934positive regulation of protein phosphorylation
GO:0002093auditory receptor cell morphogenesis
GO:0002551mast cell chemotaxis
GO:0003382epithelial cell morphogenesis
GO:0006897endocytosis
GO:0006911phagocytosis, engulfment
GO:0006935chemotaxis
GO:0006972hyperosmotic response
GO:0007010cytoskeleton organization
GO:0007015actin filament organization
GO:0007155cell adhesion
GO:0007186G-protein coupled receptor signaling pathway
GO:0007411axon guidance
GO:0008283cell proliferation
GO:0008361regulation of cell size
GO:0010592positive regulation of lamellipodium assembly
GO:0010762regulation of fibroblast migration
GO:0010811positive regulation of cell-substrate adhesion
GO:0014041regulation of neuron maturation
GO:0016358dendrite development
GO:0016477cell migration
GO:0016601Rac protein signal transduction
GO:0021799cerebral cortex radially oriented cell migration
GO:0021831embryonic olfactory bulb interneuron precursor migration
GO:0021894cerebral cortex GABAergic interneuron development
GO:0022604regulation of cell morphogenesis
GO:0030032lamellipodium assembly
GO:0030036actin cytoskeleton organization
GO:0030041actin filament polymerization
GO:0030334regulation of cell migration
GO:0030838positive regulation of actin filament polymerization
GO:0031529ruffle organization
GO:0032707negative regulation of interleukin-23 production
GO:0034446substrate adhesion-dependent cell spreading
GO:0035567non-canonical Wnt signaling pathway
GO:0043552positive regulation of phosphatidylinositol 3-kinase activity
GO:0043652engulfment of apoptotic cell
GO:0045216cell-cell junction organization
GO:0045453bone resorption
GO:0045740positive regulation of DNA replication
GO:0048168regulation of neuronal synaptic plasticity
GO:0048532anatomical structure arrangement
GO:0048812neuron projection morphogenesis
GO:0048813dendrite morphogenesis
GO:0048870cell motility
GO:0048873homeostasis of number of cells within a tissue
GO:0051496positive regulation of stress fiber assembly
GO:0051668localization within membrane
GO:0051894positive regulation of focal adhesion assembly
GO:0051932synaptic transmission, GABAergic
GO:0060071Wnt signaling pathway, planar cell polarity pathway
GO:0060263regulation of respiratory burst
GO:0060999positive regulation of dendritic spine development
GO:0071260cellular response to mechanical stimulus
GO:0071526semaphorin-plexin signaling pathway
GO:0071542dopaminergic neuron differentiation
GO:0072659protein localization to plasma membrane
GO:0090023positive regulation of neutrophil chemotaxis
GO:0090103cochlea morphogenesis
GO:0097178ruffle assembly
GO:1900026positive regulation of substrate adhesion-dependent cell spreading
GO:1904948midbrain dopaminergic neuron differentiation
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005802trans-Golgi network
GO:0005829cytosol
GO:0005884actin filament
GO:0005886plasma membrane
GO:0000139Golgi membrane
GO:0000242pericentriolar material
GO:0001891phagocytic cup
GO:0005925focal adhesion
GO:0016020membrane
GO:0019897extrinsic component of plasma membrane
GO:0030027lamellipodium
GO:0031012extracellular matrix
GO:0031410cytoplasmic vesicle
GO:0031901early endosome membrane
GO:0032587ruffle membrane
GO:0036464cytoplasmic ribonucleoprotein granule
GO:0042470melanosome
GO:0042995cell projection
GO:0043197dendritic spine
GO:0060091kinocilium
GO:0070062extracellular exosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00449RASTRNSFRMNGcoord: 44..66
score: 44.82
coord: 154..176
score: 33.72
coord: 4..25
score: 44.89
coord: 27..43
score: 26.73
coord: 106..119
score: 50.52
NoneNo IPR availableSMARTSM00173ras_sub_4coord: 1..179
e-value: 2.8E-21
score: 86.7
NoneNo IPR availableSMARTSM00175rab_sub_5coord: 4..179
e-value: 2.9E-20
score: 83.3
NoneNo IPR availableSMARTSM00174rho_sub_3coord: 6..179
e-value: 1.5E-89
score: 313.5
NoneNo IPR availableSMARTSM00176ran_sub_2coord: 9..190
e-value: 4.4E-4
score: 9.5
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 1..181
e-value: 1.2E-62
score: 212.4
NoneNo IPR availablePANTHERPTHR24072:SF291coord: 1..190
NoneNo IPR availablePANTHERPTHR24072RHO FAMILY GTPASEcoord: 1..190
NoneNo IPR availableCDDcd00157Rhocoord: 4..175
e-value: 6.12899E-97
score: 276.347
IPR001806Small GTPasePFAMPF00071Rascoord: 5..177
e-value: 2.9E-52
score: 176.5
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 1..170
e-value: 4.2E-32
score: 109.2
IPR003578Small GTPase RhoPROSITEPS51420RHOcoord: 1..176
score: 23.114
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 3..176