Collagen type IV alpha 2 chain

Overview
NameCollagen type IV alpha 2 chain
Smed IDSMED30003562
Length (bp)5140
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Collagen type IV alpha 2 chain (SMED30003562) t-SNE clustered cells

Violin plots show distribution of expression levels for Collagen type IV alpha 2 chain (SMED30003562) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Collagen type IV alpha 2 chain (SMED30003562) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Collagen type IV alpha 2 chain (SMED30003562) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003562

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 31

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30003562h1SMcG0016741 dd_Smed_v4_701_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003562h1SMcG0016741 dd_Smed_v4_701_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003562h1SMcG0016741 dd_Smed_v4_701_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30003562h1SMcG0016741 dd_Smed_v4_701_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig48629newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig48629uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig48629newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig48629uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig46804newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig41816uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig41816newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig11374newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig11374uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig11374newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig11374uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016742 Contig11374newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016742 Contig11374uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig41816uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig41816newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016742 Contig45181uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016742 Contig45181newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig46098uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig46098newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig45181uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016741 Contig45181newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig45181uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003562h1SMcG0016740 Contig45181newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
enteric muscle cellSMED30003562h1SMcG0016741 dd_Smed_v4_701_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30003562h1SMcG0016741 dd_Smed_v6_701_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
musculature systemSMED30003562h1SMcG0016741 dd_Smed_v6_701_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003562h1SMcG0016741 dd_Smed_v4_701_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Human
Match: COL24A1 (collagen type XXIV alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:20821])

HSP 1 Score: 143.665 bits (361), Expect = 1.799e-33
Identity = 242/758 (31.93%), Postives = 327/758 (43.14%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDL---GPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLG------RKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLM---------GSRGPQGVSGEPGSPGDAGVN-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQ--SFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELS 4696
            +G PG+ G  GLPG+PG+ G +G +GP G++   GP G  G  G QG  GA+G  G  G +G  G+ G  G  G  G++G +G  G +G PG  G  G  G  G  G  G  G +G  G  G  G +G+ G+ G      +KG  G IGP GE G  GP GK G  G +G  G +G+ G  GLM         G RG QG  G  G PG  G       +    G  GP G  G  G  G+ G  G +G KG +G+ G  GA G+ G  G PG  G  G +G+ G +G KG  G  GP G+ G+ G  G +G KG++G  G  G  G+ G  GP G +G  GI+G PGR G  G  G PG  G                           S GLPG  G+QGP          GE+G  G PGI G  G RG  G  G                             G  G  G+PG+ G + L G +G PG  G  G  G  G  G  GP G RGN GP G  G++GP G  G  GE                                R        I+   E ++     S +    + +D ++ +F  ++ ++N +  I+ P G  +D+ AR C D+    ++ +DG+Y+IDPN G   D+IEV C FS   +TC+  V                    +  +LE  + +            QMNFL   +S++   I   C  +  +  ++ S
Sbjct:  977 QGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSRGPPGKIGKSGPKGARGTRGAVGHLGLMGPDGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPGEDSTVLGPPGPRGEPGPVGDQGERGEPGAEGYKGHVGVPGLRGATGQQGPPGEPGDQGEQGLKGERGSEGNKGKKGAPGPSGKPGIPGLQGLLGPKGIQGYHGADGISGNPGKIGPPGKQGLPGIRGGPGRTGLAGAPGPPGVKG---------------------------SSGLPGSPGIQGP---------KGEQGLPGQPGIQGKRGHRGAQGDQGPC---------------------------GDPGLKGQPGEYGVQGLTGFQGFPGPKGPEGDAGIVGISGPKGPIGHRGNTGPLGREGIIGPTGRTGPRGE-----KGFRGETGPQGPRGQPGPPGPPGAPGPRKQMDINAAIQALIESNTALQMESYQNTEVTLIDHSEEIFKTLNYLSNLLHSIKNPLG-TRDNPARICKDLLNCEQKVSDGKYWIDPNLGCPSDAIEVFCNFSAGGQTCLPPV--------------------SVTKLEFGVGK-----------VQMNFLHLLSSEATHIITIHCLNTPRWTSTQTS 1634          

HSP 2 Score: 115.546 bits (288), Expect = 6.041e-25
Identity = 204/523 (39.01%), Postives = 250/523 (47.80%), Query Frame = 2
Query: 2537 IDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXX----------------XXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNP------GPKGETGLMGPIGPVGEDG 4039
            + G  G+ GP G  GP+GQ G+ GP G+ G +G  G  GE G +G RG  G  G TG+ GERG  G  G  G  G  G                           ++G+ G  G +G  G +GP GE G  GP G  G  G +GE G KG  G  G +G  G  G+ GEPG+PG+ G+                G  G++G+PG  G  GE G KG++GL G  G  GR G+ G PG  G  G  GQ G  G KGD G +GP GE G     GP G  G  G +G  G +G+ G+ G  G +GE GI G  G +G+ GP GLPGP G  G  G+D     P G                    G  G VG PGL G  G QGP G  G QGE G +G+RG  G  G  G  GP+G  GI G  G  G  G  G  G  G  G  G  G  GK G PG +G     G+ G+PG  G  GS G PG PG+ GP+G++G P      G +G  G  G  GP G+ G
Sbjct:  907 LPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTG---------------------------DRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQ---------------GKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVG---SRGPPGKIGKSGPKGARGTRGAVGHLGLMGPDGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPGEDSTVLGPPGPRGEPGPVGDQGERGEPGAEGYKGHVGVPGLRGATGQQGPPGEPGDQGEQGLKGERGSEGNKGKKGAPGPSGKPGIPGLQGLLGPKGIQGYHGADGISGNPGKIGPPGKQGLPGIRGGPGRTGLAGAPGPPGVKGSSGLPGSPGIQGPKGEQGLPGQPGIQGKRGHRGAQGDQGPCGDPG 1384          

HSP 3 Score: 110.923 bits (276), Expect = 1.493e-23
Identity = 175/514 (34.05%), Postives = 214/514 (41.63%), Query Frame = 2
Query: 2534 GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKG---SVGPIGPDGEKGDVGPLGKTG---------LKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPG------RIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            G  G+EG IG  G+LGPRG+   PG +G  G  G  G  GE+G +G+ GK G  GP G  G                                             E G+ G +G KG   S GP+GP GEKG +G  G  G         L G  G  G  GS+G  G  GSRGP G+ GE G  G  G                               +G++G +G  GLIG TG PG      + G  G PG TG  G  G+PG++G +GD   +GP GE G++                           GP G EGE G+QG PG KG++G  G  G  G  G  G+ GAPG                 GL GK GL+G  GG G  GE GE+G+ G PGI G +G+ G  G                                G +G VG PGQ+G    KG KG  G  G VGS+G PG  G +GP+G RG  G  G  GLMGP G
Sbjct:  804 GTQGEEGPIGAFGELGPRGK---PGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPG---------------------------------------------EVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKG------------------------------EKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGD---VGPPGEMGME---------------------------GPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEE---------------GLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTG------------------------------LPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSRGPPGKIGKSGPKGARGTRGAVGHLGLMGPDG 1164          

HSP 4 Score: 109.768 bits (273), Expect = 3.941e-23
Identity = 227/577 (39.34%), Postives = 274/577 (47.49%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---------------PGPQGIRGARGQSGPTGELGSK---------------GDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDV------GPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGS------------------PGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEK---GVKGDIGLIGPTGAP------GRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNM---GQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGK---RGNPGPKGETGLMG 4012
            GP G  GP+G+PG  G+ G +G  G KGD G +G +G                PGP G+RG  G SG TG+ G +               G  G+ GP+GP G  G RG PG  G  G  G  GPIG  G                     E G RG  G+KG  G  GP+G KG+V      G +G+TG  G  GEVG+ GS G+ G  GS GP G  GE G                   PG  G  G  G QG +G +G  GP G+ G  G  G +GEK   G +G  GLIG TG P      G+ G  G PG TG  G  G+PG++G +GD   +GP GE G++                           GP G EGE G+QG PG KG++G  G  G  G  G  G+ GAPG                 GL GK GL+G  GG G  GE GE+G+ G PGI G +   GQ G  GP GIVG  G RG+PG  G  G IG  G  GSRG  GK+G+ G KG R   G +G+ G +G +G  GEPG PG  G QG+    G PGPKGE G  G
Sbjct:  687 GPVGPIGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYPGDKGAVGLPGPPGMRGKSGPSGQTGDPGLQGPSGPPGPEGFPGDIGIPGQNGPEGPKGLLGNRGPPGPPGLKGTQGEEGPIGAFG---------------------ELGPRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGD---VGPPGEMGME---------------------------GPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEE---------------GLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSRGPPGKIGKSGPKGAR---GTRGAVGHLGLMGPDGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPG 1194          

HSP 5 Score: 100.523 bits (249), Expect = 2.484e-20
Identity = 202/550 (36.73%), Postives = 253/550 (46.00%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGE---LGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGE---------------KGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGE---PGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXX------------XXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GPPG +G  GL G PG+ G  G  G  G  G  G  G PG QG+ G  G  GP G    +GS G+ G+ GP+G  G  G RG  G                                        KG +G  G  G  GP G DG                +G VGP+G  G  G  G +G+ G++G  G+ G +G QG +GE   PG PGD G  G  G  G +G  GP+G  G  G+ G  GP G +G  GDIG+      PG+ G  GP G  G  G  G PG+KGT+G+ G +G  GE G                RGKPG++G  G  GP+G++GE+G QG  G+ GE GP+GLPG VG +G  G+ G  G              G  G  G  GP+G  GLPG  G +GP G   +QG  G+RG RGP G+ G  G +G  G  G                         +G RG  G +G+ G  GER  +G  G  G  G+ G +G PG PGL G Q   G+ GP GE G+ GP G  GE G
Sbjct:  561 GPPGMQGDKGLKGHPGLPGLPGEQGIPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFIGSPGEAGQLGPEGERGIPGIRGKKGF---------------------------------------KGRQGFPGDFGDRGPAGLDGSPGLVGGTGPPGFPGLRGSVGPVGPIGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYPGDKGAVGLPGPPGMRGKSGPSGQTGDPGLQGPSGPPGPEGFPGDIGI------PGQNGPEGPKGLLGNRGPPGPPGLKGTQGEEGPIGAFGELG---------------PRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPG---SQGPKGQRGSRGPDGLLGEQGIQGAKGEKG------------------------DQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQ---GDVGPPGEMGMEGPPGTEGESG 1020          

HSP 6 Score: 81.2629 bits (199), Expect = 1.769e-14
Identity = 172/521 (33.01%), Postives = 216/521 (41.46%), Query Frame = 2
Query: 2504 RGPSGLP---GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGP G+P   G PG+ G  G  GPKGD G       PG +G +G  G  GP G  G KG +G  G  G  G++G                                 + G  G  G  G  G +GL G +G+ GP      IG  GE G +GP G+ G+ G RG+ G KG +G  G  G RGP G+ G PG                              + G  GP G  G++G +G +GP      IG AG PG  G SG +G PG+KG KG+ G  G  GE G                                G +G +G+ GPPG +G+ GP G  G             PG  G SG  G +G  G  G+PG+ G +GP      +G  G RG  GPPG+ G  G+ GP G  G +                           G  GK G+ G  GE   +G+KG  G  G +G  GE GP GL G  G  G+ G KGE G  GP+GP GE G
Sbjct:  512 RGPRGIPGPHGNPGLPGLPGPKGPKGDPGFSPGQPVPGEKGDQGLSGLMGPPGMQGDKGLKGHPGLPGLPGEQG---------------------------------IPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFIGSPGEAGQLGPEGERGIPGIRGKKGFKGRQGFPGDFGDRGPAGLDGSPG------------------------------LVGGTGPPGFPGLRGSVGPVGP------IGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYP------------------------------GDKGAVGLPGPPGMRGKSGPSGQTG------------DPGLQGPSGPPGPEGFPGDIGIPGQNGPEGP------KGLLGNRGPPGPPGLKGTQGEEGPIGAFGEL---------------------------GPRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKG 888          

HSP 7 Score: 70.4774 bits (171), Expect = 3.821e-11
Identity = 190/552 (34.42%), Postives = 235/552 (42.57%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGG------------SGTQGEAG------ERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGI------KGSPGTIGAV---GSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RGP G  GP G PG PG+ G +G   PKGD G       PG +G +G  G  GP G  G KG +G  G  G  G++G                                 + G  G  G  G  G +GL G +G+ GP      IG  GE G +GP G+ G+ G RG+ G KG +G  G  G RGP G+ G PG                              + G  GP G  G++G +G +GP      IG AG PG  G SG +G PG+KG KG+ G  G  GE G                                G +G +G+ GPPG +G+ GP G  G             PG  G SG  G +G  G  G+PG+ G +GP G              GTQGE G      E G RG PG  G  G+ GP G  G VG  G  G+ G  GP+G  G  G  GS G  G+ G PG  G +  KG+       G PG IG +   G  G  GPPG  GP+G+RG+ GP G  G  G  G  GE G+
Sbjct:  512 RGPRGIPGPHGNPGLPGLPGPKG---PKGDPGFSPGQPVPGEKGDQGLSGLMGPPGMQGDKGLKGHPGLPGLPGEQG---------------------------------IPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFIGSPGEAGQLGPEGERGIPGIRGKKGFKGRQGFPGDFGDRGPAGLDGSPG------------------------------LVGGTGPPGFPGLRGSVGPVGP------IGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYP------------------------------GDKGAVGLPGPPGMRGKSGPSGQTG------------DPGLQGPSGPPGPEGFPGDIGIPGQNGPEGPKGLLGNRGPPGPPGLKGTQGEEGPIGAFGELGPRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGD 949          

HSP 8 Score: 63.5438 bits (153), Expect = 3.803e-9
Identity = 48/108 (44.44%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGV------KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            YKG  G+ G  GA G  GP G+ G +GE G+KG       KG+ G  GP G  GI G++G +G +G  G  G DG  G+ G +GPPG  G PGI+G  G  G  G  G
Sbjct: 1226 YKGHVGVPGLRGATGQQGPPGEPGDQGEQGLKGERGSEGNKGKKGAPGPSGKPGIPGLQGLLGPKGIQGYHGADGISGNPGKIGPPGKQGLPGIRGGPGRTGLAGAPG 1333          

HSP 9 Score: 62.3882 bits (150), Expect = 8.906e-9
Identity = 46/94 (48.94%), Postives = 55/94 (58.51%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQG---PRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            G +G RG  GA+GD GP G  G +G+ G  GV+G  G QG   P+GPEG AG+ G  G +G  G RG  G  G EG +GP G  G  G KG RG
Sbjct: 1363 GIQGKRGHRGAQGDQGPCGDPGLKGQPGEYGVQGLTGFQGFPGPKGPEGDAGIVGISGPKGPIGHRGNTGPLGREGIIGPTGRTGPRGEKGFRG 1456          

HSP 10 Score: 59.6918 bits (143), Expect = 7.075e-8
Identity = 48/102 (47.06%), Postives = 58/102 (56.86%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            + GARG  GS G KG  G  G  G  GE G++G KGE G QG RGP G+ G  G+    GE G +GK G +G  GS G  G  G PG++G +G VG  G  G
Sbjct:  910 HVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGDQGKRGPHGLIGKTGN---PGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMG 1008          

HSP 11 Score: 56.9954 bits (136), Expect = 3.944e-7
Identity = 47/118 (39.83%), Postives = 64/118 (54.24%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV------------KGDIGIQGETGPRGKDGNK---GDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G++G  GAKGD G +G VG  GE G++G  G  G +G +G +G+ GV            KG++G+ G  GP G+ G     G EG VG PG  G PG KG +G +G  G  G +
Sbjct: 1016 EGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSR 1133          

HSP 12 Score: 56.6102 bits (135), Expect = 5.619e-7
Identity = 43/102 (42.16%), Postives = 56/102 (54.90%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G RG +G  G +G  G +G  G  GE G++G++G+ G  G  G EG  G +G+ G+QGE G +G  G  G  G  G PG  G PG  G  G    QG+DG K
Sbjct:  966 GERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEG---LQGKDGLK 1064          

HSP 13 Score: 55.8398 bits (133), Expect = 1.013e-6
Identity = 42/102 (41.18%), Postives = 56/102 (54.90%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +GA+G +G  G +G HG  G+ G  GE G +G  G  G+ G  G  G+ G  G  G+QG+ GP G+ G +G  G+ G  G  G PG KG  G  G+ G  GE
Sbjct:  941 QGAKGEKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGE 1042          

HSP 14 Score: 55.4546 bits (132), Expect = 1.361e-6
Identity = 45/110 (40.91%), Postives = 61/110 (55.45%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            ++G  G++G  G+ GD G  G+ G RG  G  G  GE G++GP G EG +G+      KGD+G  G  G  G+ G +G+ G+ G  G  G  G+KG  G  G  G DGEK
Sbjct:  970 FQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQGKDGLKGVPGGRGLPGEDGEK 1079          

HSP 15 Score: 55.0694 bits (131), Expect = 1.557e-6
Identity = 113/275 (41.09%), Postives = 131/275 (47.64%), Query Frame = 2
Query: 3215 PGRIGEAGPPGKTGASGAEGQPGVKGTKGDTG------ILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPP------GLTGPQGKRGNPGPKGETG 4003
             G+ G  G PG  G  G  G PG KG KGD G      + G +G+QGL G +GP G +G KG +G PG  G  G  G  G  G +G  G PGR+G  GP G PGP G  G                          G PG+AG  GP G  G  G  G++G +G  G  G+ G RGP G  G  G  G  G PG  G  G +G  GP G  G+ G +G  G KG   L GIKG  G  G  G  GEPG P      GL GP G RG  GP G+TG
Sbjct:  509 SGKRGPRGIPGPHGNPGLPGLPGPKGPKGDPGFSPGQPVPGEKGDQGLSGLMGPPGMQGDKGLKGHPGLPGLPGEQGIPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFI------------------------GSPGEAGQLGPEGERGIPGIRGKKGFKGRQGFPGDFGDRGPAGLDGSPGLVGGTGPPGFPGLRGSVGPVGPIGPAGIPGPMGLSGNKG---LPGIKGDKGEQGTAGELGEPGYPGDKGAVGLPGPPGMRGKSGPSGQTG 756          

HSP 16 Score: 54.299 bits (129), Expect = 2.537e-6
Identity = 48/108 (44.44%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDI---GIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGS---RGPVGNQGRDGE 2134
            +G +G RGS G KG  G  G  G  G  G++G+ G  GIQG  G +GI+G  G I   G QG  G RG  G  G  G+ GPPG  G+ G+ GS   +GP G QG  G+
Sbjct: 1254 QGLKGERGSEGNKGKKGAPGPSGKPGIPGLQGLLGPKGIQGYHGADGISGNPGKIGPPGKQGLPGIRGGPGRTGLAGAPGPPGVKGSSGLPGSPGIQGPKGEQGLPGQ 1361          

HSP 17 Score: 53.9138 bits (128), Expect = 4.032e-6
Identity = 45/99 (45.45%), Postives = 55/99 (55.56%), Query Frame = 2
Query: 1859 GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGI------QGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G KGD G SG +G  G  G KG+KG  G+ G  G +GI G  G+IG       QG  GP G  G KG +G +G PG AG  G +G RG  G +G+ G K
Sbjct:  549 GEKGDQGLSGLMGPPGMQGDKGLKGHPGLPGLPGEQGIPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFIGSPGEAGQLGPEGERGIPGIRGKKGFK 647          

HSP 18 Score: 53.5286 bits (127), Expect = 4.813e-6
Identity = 44/103 (42.72%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKG---DIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G +G  G  G+ G  GP G+ G  G+ G++G +G  G QG +GP G  G+KG   + G+QG TG +G  G KG EG  G  G +G  G  G RG  G  GR+G
Sbjct: 1336 GVKGSSGLPGSPGIQGPKGEQGLPGQPGIQGKRGHRGAQGDQGPCGDPGLKGQPGEYGVQGLTGFQGFPGPKGPEGDAGIVGISGPKGPIGHRGNTGPLGREG 1438          

HSP 19 Score: 53.5286 bits (127), Expect = 5.415e-6
Identity = 98/292 (33.56%), Postives = 122/292 (41.78%), Query Frame = 2
Query: 3167 EKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            ++G +G  G  G  G PG  G  GP G  G S  +  PG KG +G +G++GP G QG             KG +G PG  G  G  G  G  G +G  G PGR+G  GP G PGP G  G                          G PG+AG  GP G  G  G  G++G +G  G  G+ G RGP                                  GLDG  G  G  G     G++GS G +G +G  G PGP GL+G +G  G  G KGE G  G +G  G  G+
Sbjct:  511 KRGPRGIPGPHGNPGLPGLPGPKGPKGDPGFSPGQPVPGEKGDQGLSGLMGPPGMQG------------DKGLKGHPGLPGLPGEQGIPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFI------------------------GSPGEAGQLGPEGERGIPGIRGKKGFKGRQGFPGDFGDRGP---------------------------------AGLDGSPGLVGGTGPPGFPGLRGSVGPVGPIGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYPGD 733          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Human
Match: COL27A1 (collagen type XXVII alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:22986])

HSP 1 Score: 120.939 bits (302), Expect = 1.425e-26
Identity = 251/794 (31.61%), Postives = 322/794 (40.55%), Query Frame = 2
Query: 2852 EKGVRGLLGRKGSVGPIGPDGEKG------------DVGPLGKTGLKGTRGEVGI---KGSEGKTGLMGS------------RGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEG-------------------------------------QPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPI---------GPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRT-PGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            E G+ G  G KG +GP+G  GE+G            D GP+G  GLKG RG+ G     G +G+ GLMG             RGP+G SG+ G  G  G  G KG QG+ G  G  G  G  G           G KG  G +GPTGAPGR+G  G PG  G  G +G                                       G +G  GD G  G  G QGL G  G  G  G  G RG PG +GS G  GP+G +G+ G    PGR+G  G  GLPGP G  G  G +G  GP G  G+ G  G  G  G  G  G  GPAG  G  G AG  G +GPPG  G  G  G  GP G                       G +G  GL G  GEPG KG+    G  G PG  G  G KG PG  G  G QG RG PG  G+ G++GP+         GP G+ G  G  G  GP GPPGP G  G PG  G      +       +      G+      S   +  +D    +F  +  ++N I+ I+ P G  K++ AR C D+    ++  DG Y++DPN G   D+IEV C F+   +TC+  +                     + ++E AISR            QMNFL   +S+  Q I   C    ++   E + +   K A      + +  + + G+                      F   +  DGC  +      + F   T D   LPII        + G     L V   CF
Sbjct: 1157 EPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPG---------TPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGA---PGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPG---------------------KRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGGPIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPLG-TKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPI--------------------TASKVEFAISR-----------VQMNFLHLLSSEVTQHITIHCLNMTVW--QEGTGQTPAKQAVRFRAWNGQIFE-AGGQ----------------------FRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSG-KQYRLEVGPACF 1859          

HSP 2 Score: 71.633 bits (174), Expect = 1.344e-11
Identity = 172/497 (34.61%), Postives = 208/497 (41.85%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPG---------PQGIRGARGQSGPTGELGSKGDRGKF---------------GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGR---------------KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGI------------KGSEGKTG-------------LMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPR---GEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQ---------GVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIV 3748
            +GPPG  G  GLPG+ G+ G  G +G  G+ G  GQ G PG         P G++G RG  GP GE G KG  G                 GP+G +G +GE+G  G++G                                     KG +G LG                KGS G +GP G  G +G  G+ GL G  G  GI            KG +G+ G              +G RG +G  G+PG PG  GV                            G RG+ G +G  G  GP G PG  G  G  G  G  G  G PG +G +G  G+ GPR   G QGL+G  GP G  G  G  G+ G QG  G+ GP          GV G  G QGPPG KGE G  G  GP G  G  G+ G PG  G  G  G  G  G  G PG AGL GP G  G  G  G +G RGPPG+   MG+ G  GP+GI+
Sbjct: 1149 KGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGA------------------------------------KGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQ---------------------------GLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGL---MGKEGIVGPLGIL 1579          

HSP 3 Score: 64.3142 bits (155), Expect = 2.685e-9
Identity = 151/414 (36.47%), Postives = 181/414 (43.72%), Query Frame = 2
Query: 2525 GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP------GKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIV 3748
            G PG+   +   G KGD+G  G SGNPGP G +G +G  GP G     G++G+ GP+G  G K                                                G  G  G  G VG  GP G +G +G  G  GL G+ GE G+ G  GK G MG  G  GV GE G PG  G                 G +G+ G PG PG  G+ GV G IG  GP G  G +G  G P      G+ G  G  G PG +G KG  G+ G  G +G  GPLG +G KG  G  G PG +G  G+ GP G  G  G++G PG +G  GP G  GP G  GP G  GAPG                 G PG+ G  G  G  G +GE G+ G  GP G  G MG  G  G  GIV
Sbjct:  676 GDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGH---PGEQGQPGPEGSPGAK------------------------------------------------GYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDG---------------NPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLE---------------GQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIV 1008          

HSP 4 Score: 63.5438 bits (153), Expect = 4.376e-9
Identity = 113/278 (40.65%), Postives = 141/278 (50.72%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGI---RGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDI---GLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILG 3319
            RG  G PG PG  G+EG  G +G  G +GQ G+PGP+G    +G++G  GP G+ G  G  G+ G QG  G  G RG  G QG  G AGP+G  G  G  G +G +G  G+PGP GP G++G  G+ G  G+ GP G  GE G  G LG  G +GT G  G+ G++G+    GS+G  G SGE G PG AG+                               G KG  GDI   G+ GP G PG +G+ G  G  G  G  G PG KG KG  G  G
Sbjct: 1354 RGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEP---GSKGQPGDSGEMGFPGMAGL------------------------------FGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWG 1598          

HSP 5 Score: 60.077 bits (144), Expect = 4.664e-8
Identity = 99/288 (34.38%), Postives = 122/288 (42.36%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---------PGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQ 3334
            G  GP G PG  G  G++G  GP GD GP+G  G          PG  G RG  G  G  G++G  G  G FG +GP G  G                                   G  G  GP G  G+ G LG  G +G  GP G KG +G +G+ GLKG +GE G+ G  G  G  G +G QG+ G PG+ G  G                              P G+ G KG IG  GP G  G  G+ GPPG  G  G +G+PG +G  G  G LGP G++
Sbjct:  724 GQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGFPGVFGERGPPGLDGN---------------------------------PGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPG------------------------------PLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDE 948          

HSP 6 Score: 59.6918 bits (143), Expect = 6.262e-8
Identity = 184/525 (35.05%), Postives = 220/525 (41.90%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGE------------LGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDG---EKGDVGPLGKTGL-------------KGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN 3979
            GP GT G  GL G+ G  G EG  GP G  G +G  G+PGP G  G +GQ G  GE             G +G  GK G +G TG KG +G  G  G  G  G  GP G  G  G +G  G  G PG  G  GE G+ G  G KG VGP+GP G   EKG+ G  GK                +G RGE G  G  G+ G+ G RG  G  G+PG PG  G                                   G KG  G  GP G   + G+AG PG+ G  G +G PG +G  G  G+ G  G  GL G  G  G +G +G  G PG  G  G  G  GV G  G QGPPG KGE G  G  GP G                             G  G+ GL G  G  G++G+ G+ G+ G PG+AG  G +GP G IG                            +G+ G  G PG  G+  + G  G  G  G  G KG+ G  G  G QG RG 
Sbjct: 1171 GPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQQGQPGHPGPRGW---------------------------------PGPKGSKGAEGPKG---KQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKR---------------------------GTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGF---------------------------KGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGP 1605          

HSP 7 Score: 58.5362 bits (140), Expect = 1.491e-7
Identity = 208/599 (34.72%), Postives = 251/599 (41.90%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPG---IDGKEGSIGPKGD---------------LGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSR---------------------------------------GPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGG---------SGTQGEAG----------------ERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GPPG  GP G+ GKPG   + G  G +GP+GD                G +G  G PG  G++G  G  G  G +G +G  G  G  G  G  GE+GD G  GPPG  GP G +G  G PG  G  G  G  GP G  G  G+RG  GR+G  GP GP GE+G  G  G  G +G  G  G +G  G+ G +GSR                                       GP G  GEPG PG+AG                                    +KGD+G +G  G  G IG+ G PG  G SG  G  G+KG +GD    GP GE G  G  G +G  G  G  G  G +G  G SG QG +G  G +G  G +G+LG +G+PG  G  G  G  G+ G  G +G  G  G  G PGL G  G +G  G           G QGE G                +RGDRG PG  G  GQ G  G                               RG  G+ G+PG  G R   G KGS G  G  G +G+ G PG  G QG +G PGP+G  G  G  G  G DG
Sbjct:  916 GPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPPGVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSRGLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPIGPPGEMGPKGPPGAVGEPGLPGEAG------------------------------------MKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGDRGDP---GPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAKGYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPLGPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQG------------------------------LRGKPGQQGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGIAGPDG 1445          

HSP 8 Score: 56.225 bits (134), Expect = 8.305e-7
Identity = 49/102 (48.04%), Postives = 63/102 (61.76%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G+ G +G KGD GP   +GT GE G+ G +GE G++G  GP G  G+KGD   +G+ GP G+ G KG EG +G  G  G PG+ G RGP G  G+ GEK
Sbjct: 1156 GEPGLPGEAGMKGDLGP---LGTPGEQGLIGQRGEPGLEGDSGPMGPDGLKGD---RGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEK 1251          

HSP 9 Score: 55.4546 bits (132), Expect = 1.115e-6
Identity = 42/103 (40.78%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +KG  G  G  G +G  GP G  G +G  G +G+ G  G  GP+G  G  G+ G  G+ G  G RG  G  G  G +G PG+   PG+ G RGP G  G  GE
Sbjct:  710 HKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGKMGMPGF---PGVFGERGPPGLDGNPGE 809          

HSP 10 Score: 55.0694 bits (131), Expect = 1.629e-6
Identity = 44/103 (42.72%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G +G SG  G  GP G+ GT G  G+ G +GE G +G  G  G  G  G  G+ G  GP G  G KG +G  GPPG  G  GI G  G +G  G  G K
Sbjct: 1486 QGPPGFKGESGLPGQLGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPK 1588          

HSP 11 Score: 54.6842 bits (130), Expect = 2.262e-6
Identity = 137/402 (34.08%), Postives = 164/402 (40.80%), Query Frame = 2
Query: 2852 EKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTK---GDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---PGPKGETGLMGPIGPVGEDG 4039
             KG  G  G  G  G  GP+G  G  G  G+ GL G  G+ G KGS G  GL       G+ G PGS G+ G+                                  GV G  G +G  G PG  GE GPPG  G  G  G PG  G     GD G+LGP G  G                              P+G++G +G  G PG KG+ G  G+PG  G  G  G  G+ G                PG PG  G  GP G  G +G  G  G  GP G  G++G  G NGP G+                      G  G+RGL G  G+ G +G+    G  G+PG  G  G KG PG PGL G +G+ G+   PGP GE G MG IG VGE G
Sbjct:  710 HKGYPGPAGHPGEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGL------PGLFGLPGSDGERGL---------------------------------PGVPGKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPG------------------------------PKGMKGLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGL---------------PGFPGARGKPGPLGKVGDKGSIGFPGPPGPEGFPGDIGPPGDNGPEGM---------------------KGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLEGQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPG 1006          

HSP 12 Score: 54.299 bits (129), Expect = 3.088e-6
Identity = 58/166 (34.94%), Postives = 79/166 (47.59%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIK 2983
            +GPPG +G SGLPG+         +GP G  G  G++G PG QG  G++GQ G +GE+G  G  G FGP+GP GD G +                                       G  G +G  GL+G++G VGP+G  G  G  GP G    KG+RG+ G++
Sbjct: 1486 QGPPGFKGESGLPGQ---------LGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFK---------------------------------------GIQGPRGPPGLMGKEGIVGPLGILGPSGLPGPKGD---KGSRGDWGLQ 1600          

HSP 13 Score: 53.9138 bits (128), Expect = 3.248e-6
Identity = 46/112 (41.07%), Postives = 58/112 (51.79%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDG---------NKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + G RG  G  G+KG  GP G+ G  G  G +GV+G  G+ GPRG   + G +G  GI G  G  G+DG         + GD G +GP G  GNPG+ G  G  G  G  GE
Sbjct: 1386 HPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRG---VVGRQGLEGIAGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGE 1494          

HSP 14 Score: 53.9138 bits (128), Expect = 4.043e-6
Identity = 46/103 (44.66%), Postives = 54/103 (52.43%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + GA+G  G  G  G+ GP G+ G +G  G  G  GE   QG  GPEG  G KG  G QG  GP G  G KG  G +G PG  G PG  G RG  G  G+ G+
Sbjct:  686 HDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHPGE---QGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGK 785          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Human
Match: COL11A1 (collagen type XI alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:2186])

HSP 1 Score: 115.161 bits (287), Expect = 8.275e-25
Identity = 68/263 (25.86%), Postives = 116/263 (44.11%), Query Frame = 2
Query: 4226 FVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGER-LGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            + D  + +F  ++ +   I+ ++FP G  + + ARTC D++L      DG+Y+IDPN+G   DS +V C F+   +TCI   K+  G+     P +   +W +  +  G +    D+    ++  QM FL+   + + Q   + C QS  +                   +D  S    K  R LG N+  M        +    F  T+ YDGC  R     +  ++ T  +  +PI+D  IND+ ++     G  V  +CF
Sbjct: 1456 YSDGMEEIFGSLNSLKQDIEHMKFPMG-TQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKPGSWFSEFK-RGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAW-------------------YDVSSGSYDKALRFLGSNDEEMS-------YDNNPFIKTL-YDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQN-QKFGFEVGPVCF 1688          

HSP 2 Score: 108.227 bits (269), Expect = 1.043e-22
Identity = 69/275 (25.09%), Postives = 114/275 (41.45%), Query Frame = 2
Query:   98 WN*NLYLYQFSGPITTMLNITSLKRGVXXXXXXXXXXXXXXXXXXTQALSYRDGV-VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLFQG-KFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
            W    +L+ F+    T L +T L                       QA   R    VD+L A+   +   G +    FC N +       A+R      L A +  LF G  FP++FSIL T K +      + SI N++    + V  GR     + D    P+   +  L    ++ DG+WHR+A SV+K +V++++DC+   +  L+R+   +  + G          +  F G +   L+ G    A+  C+ Y+PDC++        Q P
Sbjct:    8 WKTKRWLWDFT---VTTLALTFL----------------------FQAREVRGAAPVDVLKALDFHNSPEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDY-PLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKAAQAQEP 256          

HSP 3 Score: 70.4774 bits (171), Expect = 3.358e-11
Identity = 51/103 (49.51%), Postives = 63/103 (61.17%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G+RG  G+KG+ G  G  G +G++G+KG +GE G  GPRG +G  G KG  G  G+ GP G+ G KG  G  G PGY G  G KGS G  G  G +GEK
Sbjct:  649 KGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEK 751          

HSP 4 Score: 60.077 bits (144), Expect = 4.516e-8
Identity = 48/108 (44.44%), Postives = 57/108 (52.78%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP---------GYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +G  G KGD G  GQ+G RGE G +G KG  G  G  GP G AG KG +G+ G  G  G+ G KG  G  G P         G AG PG +G RGP G +G  G +
Sbjct:  671 GFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGAR 778          

HSP 5 Score: 56.6102 bits (135), Expect = 5.654e-7
Identity = 137/437 (31.35%), Postives = 165/437 (37.76%), Query Frame = 2
Query: 2585 RGQSGNPGPQGIR------------------GARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG---------------------------------IKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG 3742
            +G+SG+PGPQG R                  GA G  G  GE G+KGDRG  G  G  GDKG R                     G  G +G  G  G+ G +G  GE G RGL G  G  G +GP G  G  G  G  G+ G  G  G                                  KG +GK GL G  G  G  G PG  G +G                 G +G  G  G  G  G +GVKG  G+ G  G+ G  GE G P      G +G  G+KG +G+ G +GPRGE G +                           GP+G  G  G  GP G+ GE G +G+PG                               PG PG+ G +G  G  G  G  GE+G RG  G  G  GQRGP GP G
Sbjct:  436 KGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHR---------------------GERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEK---------------GALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFP------GFKGDMGLKGDRGEVGQIGPRGEDGPE---------------------------GPKGRAGPTGDPGPSGQAGEKGKLGVPG------------------------------LPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRG 773          

HSP 6 Score: 55.0694 bits (131), Expect = 1.676e-6
Identity = 41/97 (42.27%), Postives = 53/97 (54.64%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            G  G +G +G  GD GPSGQ G +G++G+ G+ G  G QGP+G  G  G  G  G +G  G  GK G +G  G  GP G       +G+RGP G  G
Sbjct:  695 GPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGS------RGARGPTGKPG 785          

HSP 7 Score: 54.6842 bits (130), Expect = 1.886e-6
Identity = 149/391 (38.11%), Postives = 178/391 (45.52%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKR---GNPGPKGETGLMGPIGPVGEDG 4039
            G  G  GP GP G +G     G TG  G RG  G  G  G  G  G++G +G  G PG PGD G                    G +G  G PGP G+ G++G+ G IGP G P   GEAGP G  G  G  G PG  G  G  G  GP+G  G  G  GP G +G  G +G PG QG  G  G +G +G+ G+ G PG  G  G  G  G  G  G  G  G  GP G  G  G  G  G  GL G  G +G  G  G +G+ G +GDRG  G  G  G+ GP GP G                          G  G  G  G+ G+KG+  + G+ G PG  G  GS G PG PG  G +G R   G PGP+G+ G  GP G  G  G
Sbjct:  437 GESGDPGPQGPRGVQGPP---GPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHR------------------GERGPQGPPGPPGDDGMRGEDGEIGPRGLP---GEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRA------------------------GPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGARG 779          

HSP 8 Score: 53.5286 bits (127), Expect = 5.095e-6
Identity = 112/296 (37.84%), Postives = 135/296 (45.61%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSG------NPGPQ------GIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            RG  G RG  G PG+PG+ G +G  GPKG++GP+G+ G      NPGPQ      G  G  G+ GP G+ G  G  G  GP G  G +G+ G+ G+ GPPGP GP G  G  G  G                    GVRGL G KG  G  G  G KGD+      GLKG RGEVG  G  G+ G  G +G  G +G+PG  G A                                    G KG +G+ G  G PGR    GP G TG  G  G  G KG +G  G  GPRG++G
Sbjct:  535 RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGA------------------DGVRGLKGSKGEKGEDGFPGFKGDM------GLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQA------------------------------------GEKGKLGVPGLPGYPGR---QGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRG 767          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Human
Match: COL11A1 (collagen type XI alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:2186])

HSP 1 Score: 114.005 bits (284), Expect = 2.115e-24
Identity = 68/263 (25.86%), Postives = 116/263 (44.11%), Query Frame = 2
Query: 4226 FVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGER-LGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            + D  + +F  ++ +   I+ ++FP G  + + ARTC D++L      DG+Y+IDPN+G   DS +V C F+   +TCI   K+  G+     P +   +W +  +  G +    D+    ++  QM FL+   + + Q   + C QS  +                   +D  S    K  R LG N+  M        +    F  T+ YDGC  R     +  ++ T  +  +PI+D  IND+ ++     G  V  +CF
Sbjct: 1533 YSDGMEEIFGSLNSLKQDIEHMKFPMG-TQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKPGSWFSEFK-RGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAW-------------------YDVSSGSYDKALRFLGSNDEEMS-------YDNNPFIKTL-YDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQN-QKFGFEVGPVCF 1765          

HSP 2 Score: 106.686 bits (265), Expect = 2.920e-22
Identity = 69/275 (25.09%), Postives = 114/275 (41.45%), Query Frame = 2
Query:   98 WN*NLYLYQFSGPITTMLNITSLKRGVXXXXXXXXXXXXXXXXXXTQALSYRDGV-VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLFQG-KFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
            W    +L+ F+    T L +T L                       QA   R    VD+L A+   +   G +    FC N +       A+R      L A +  LF G  FP++FSIL T K +      + SI N++    + V  GR     + D    P+   +  L    ++ DG+WHR+A SV+K +V++++DC+   +  L+R+   +  + G          +  F G +   L+ G    A+  C+ Y+PDC++        Q P
Sbjct:    8 WKTKRWLWDFT---VTTLALTFL----------------------FQAREVRGAAPVDVLKALDFHNSPEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDY-PLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKAAQAQEP 256          

HSP 3 Score: 70.4774 bits (171), Expect = 2.961e-11
Identity = 51/103 (49.51%), Postives = 63/103 (61.17%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G+RG  G+KG+ G  G  G +G++G+KG +GE G  GPRG +G  G KG  G  G+ GP G+ G KG  G  G PGY G  G KGS G  G  G +GEK
Sbjct:  726 KGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEK 828          

HSP 4 Score: 60.4622 bits (145), Expect = 4.217e-8
Identity = 48/108 (44.44%), Postives = 57/108 (52.78%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP---------GYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +G  G KGD G  GQ+G RGE G +G KG  G  G  GP G AG KG +G+ G  G  G+ G KG  G  G P         G AG PG +G RGP G +G  G +
Sbjct:  748 GFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGAR 855          

HSP 5 Score: 57.3806 bits (137), Expect = 3.210e-7
Identity = 137/437 (31.35%), Postives = 165/437 (37.76%), Query Frame = 2
Query: 2585 RGQSGNPGPQGIR------------------GARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG---------------------------------IKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG 3742
            +G+SG+PGPQG R                  GA G  G  GE G+KGDRG  G  G  GDKG R                     G  G +G  G  G+ G +G  GE G RGL G  G  G +GP G  G  G  G  G+ G  G  G                                  KG +GK GL G  G  G  G PG  G +G                 G +G  G  G  G  G +GVKG  G+ G  G+ G  GE G P      G +G  G+KG +G+ G +GPRGE G +                           GP+G  G  G  GP G+ GE G +G+PG                               PG PG+ G +G  G  G  G  GE+G RG  G  G  GQRGP GP G
Sbjct:  513 KGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHR---------------------GERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEK---------------GALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFP------GFKGDMGLKGDRGEVGQIGPRGEDGPE---------------------------GPKGRAGPTGDPGPSGQAGEKGKLGVPG------------------------------LPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRG 850          

HSP 6 Score: 55.8398 bits (133), Expect = 8.315e-7
Identity = 149/391 (38.11%), Postives = 178/391 (45.52%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKR---GNPGPKGETGLMGPIGPVGEDG 4039
            G  G  GP GP G +G     G TG  G RG  G  G  G  G  G++G +G  G PG PGD G                    G +G  G PGP G+ G++G+ G IGP G P   GEAGP G  G  G  G PG  G  G  G  GP+G  G  G  GP G +G  G +G PG QG  G  G +G +G+ G+ G PG  G  G  G  G  G  G  G  G  GP G  G  G  G  G  GL G  G +G  G  G +G+ G +GDRG  G  G  G+ GP GP G                          G  G  G  G+ G+KG+  + G+ G PG  G  GS G PG PG  G +G R   G PGP+G+ G  GP G  G  G
Sbjct:  514 GESGDPGPQGPRGVQGPP---GPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHR------------------GERGPQGPPGPPGDDGMRGEDGEIGPRGLP---GEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRA------------------------GPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGARG 856          

HSP 7 Score: 55.0694 bits (131), Expect = 1.577e-6
Identity = 41/93 (44.09%), Postives = 52/93 (55.91%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIK 2092
            G  G +G +G  GD GPSGQ G +G++G+ G+ G  G QGP+G  G  G       KG  G+ G+ GPRG+ G  G  GS G  G  G PG K
Sbjct:  772 GPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGARGPTGKPGPK 864          

HSP 8 Score: 54.299 bits (129), Expect = 3.067e-6
Identity = 112/296 (37.84%), Postives = 135/296 (45.61%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSG------NPGPQ------GIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            RG  G RG  G PG+PG+ G +G  GPKG++GP+G+ G      NPGPQ      G  G  G+ GP G+ G  G  G  GP G  G +G+ G+ G+ GPPGP GP G  G  G  G                    GVRGL G KG  G  G  G KGD+      GLKG RGEVG  G  G+ G  G +G  G +G+PG  G A                                    G KG +G+ G  G PGR    GP G TG  G  G  G KG +G  G  GPRG++G
Sbjct:  612 RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGA------------------DGVRGLKGSKGEKGEDGFPGFKGDM------GLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQA------------------------------------GEKGKLGVPGLPGYPGR---QGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRG 844          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Human
Match: COL11A1 (collagen type XI alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:2186])

HSP 1 Score: 113.62 bits (283), Expect = 2.288e-24
Identity = 68/263 (25.86%), Postives = 116/263 (44.11%), Query Frame = 2
Query: 4226 FVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGER-LGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            + D  + +F  ++ +   I+ ++FP G  + + ARTC D++L      DG+Y+IDPN+G   DS +V C F+   +TCI   K+  G+     P +   +W +  +  G +    D+    ++  QM FL+   + + Q   + C QS  +                   +D  S    K  R LG N+  M        +    F  T+ YDGC  R     +  ++ T  +  +PI+D  IND+ ++     G  V  +CF
Sbjct: 1584 YSDGMEEIFGSLNSLKQDIEHMKFPMG-TQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKPGSWFSEFK-RGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAW-------------------YDVSSGSYDKALRFLGSNDEEMS-------YDNNPFIKTL-YDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDFGDQN-QKFGFEVGPVCF 1816          

HSP 2 Score: 107.071 bits (266), Expect = 2.828e-22
Identity = 69/275 (25.09%), Postives = 114/275 (41.45%), Query Frame = 2
Query:   98 WN*NLYLYQFSGPITTMLNITSLKRGVXXXXXXXXXXXXXXXXXXTQALSYRDGV-VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLFQG-KFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
            W    +L+ F+    T L +T L                       QA   R    VD+L A+   +   G +    FC N +       A+R      L A +  LF G  FP++FSIL T K +      + SI N++    + V  GR     + D    P+   +  L    ++ DG+WHR+A SV+K +V++++DC+   +  L+R+   +  + G          +  F G +   L+ G    A+  C+ Y+PDC++        Q P
Sbjct:    8 WKTKRWLWDFT---VTTLALTFL----------------------FQAREVRGAAPVDVLKALDFHNSPEGISKTTGFCTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIYNEHGIQQIGVEVGRSPVFLFEDHTGKPAPEDY-PLFRTVNIADGKWHRVAISVEKKTVTMIVDCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYSPDCDSSAPKAAQAQEP 256          

HSP 3 Score: 70.8626 bits (172), Expect = 2.516e-11
Identity = 51/103 (49.51%), Postives = 63/103 (61.17%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G+RG  G+KG+ G  G  G +G++G+KG +GE G  GPRG +G  G KG  G  G+ GP G+ G KG  G  G PGY G  G KGS G  G  G +GEK
Sbjct:  777 KGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEK 879          

HSP 4 Score: 60.4622 bits (145), Expect = 3.671e-8
Identity = 48/108 (44.44%), Postives = 57/108 (52.78%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP---------GYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +G  G KGD G  GQ+G RGE G +G KG  G  G  GP G AG KG +G+ G  G  G+ G KG  G  G P         G AG PG +G RGP G +G  G +
Sbjct:  799 GFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGAR 906          

HSP 5 Score: 57.7658 bits (138), Expect = 2.321e-7
Identity = 137/437 (31.35%), Postives = 165/437 (37.76%), Query Frame = 2
Query: 2585 RGQSGNPGPQGIR------------------GARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG---------------------------------IKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG 3742
            +G+SG+PGPQG R                  GA G  G  GE G+KGDRG  G  G  GDKG R                     G  G +G  G  G+ G +G  GE G RGL G  G  G +GP G  G  G  G  G+ G  G  G                                  KG +GK GL G  G  G  G PG  G +G                 G +G  G  G  G  G +GVKG  G+ G  G+ G  GE G P      G +G  G+KG +G+ G +GPRGE G +                           GP+G  G  G  GP G+ GE G +G+PG                               PG PG+ G +G  G  G  G  GE+G RG  G  G  GQRGP GP G
Sbjct:  564 KGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHR---------------------GERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEK---------------GALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFP------GFKGDMGLKGDRGEVGQIGPRGEDGPE---------------------------GPKGRAGPTGDPGPSGQAGEKGKLGVPG------------------------------LPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRG 901          

HSP 6 Score: 56.6102 bits (135), Expect = 6.114e-7
Identity = 149/391 (38.11%), Postives = 178/391 (45.52%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKR---GNPGPKGETGLMGPIGPVGEDG 4039
            G  G  GP GP G +G     G TG  G RG  G  G  G  G  G++G +G  G PG PGD G                    G +G  G PGP G+ G++G+ G IGP G P   GEAGP G  G  G  G PG  G  G  G  GP+G  G  G  GP G +G  G +G PG QG  G  G +G +G+ G+ G PG  G  G  G  G  G  G  G  G  GP G  G  G  G  G  GL G  G +G  G  G +G+ G +GDRG  G  G  G+ GP GP G                          G  G  G  G+ G+KG+  + G+ G PG  G  GS G PG PG  G +G R   G PGP+G+ G  GP G  G  G
Sbjct:  565 GESGDPGPQGPRGVQGPP---GPTGKPGKRGRPGADGGRGMPGEPGAKGDRGFDGLPGLPGDKGHR------------------GERGPQGPPGPPGDDGMRGEDGEIGPRGLP---GEAGPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRA------------------------GPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGSRGARG 907          

HSP 7 Score: 55.0694 bits (131), Expect = 1.443e-6
Identity = 41/97 (42.27%), Postives = 53/97 (54.64%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            G  G +G +G  GD GPSGQ G +G++G+ G+ G  G QGP+G  G  G  G  G +G  G  GK G +G  G  GP G       +G+RGP G  G
Sbjct:  823 GPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPRGS------RGARGPTGKPG 913          

HSP 8 Score: 54.6842 bits (130), Expect = 2.432e-6
Identity = 112/296 (37.84%), Postives = 135/296 (45.61%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSG------NPGPQ------GIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            RG  G RG  G PG+PG+ G +G  GPKG++GP+G+ G      NPGPQ      G  G  G+ GP G+ G  G  G  GP G  G +G+ G+ G+ GPPGP GP G  G  G  G                    GVRGL G KG  G  G  G KGD+      GLKG RGEVG  G  G+ G  G +G  G +G+PG  G A                                    G KG +G+ G  G PGR    GP G TG  G  G  G KG +G  G  GPRG++G
Sbjct:  663 RGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGA------------------DGVRGLKGSKGEKGEDGFPGFKGDM------GLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPSGQA------------------------------------GEKGKLGVPGLPGYPGR---QGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRG 895          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Celegans
Match: col-135 (COLlagen [Source:UniProtKB/TrEMBL;Acc:G3MU58])

HSP 1 Score: 119.013 bits (297), Expect = 7.147e-27
Identity = 209/533 (39.21%), Postives = 263/533 (49.34%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGP---------TGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAG---LQGPAGGSG------TQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGE------PGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            +G PG +G  G  G+ G +G   S G KGD G +G  GNPGP+G +GA G  G  G  GSKGD+G  G +G          TGDKGE+G  G++G  G  G                                G +G  G KG+ G  G DG  G  G  G  G KG  G  G KG +G+ GL G +G +G  G PG+ GD G  G  G +G  G+ G  G  G +G+ G       KG KG  G  G  G PG  G+ G  G  GA GA+G PG KG KG+ G+ GP+G++G DG  G  G KG KG  G  G  G+ G+ G +G +G  G  G PG+KG+ G  GL GP G  G  G  GA G  G  G  G  G  G+PG  G  G   L GP G  G      ++G+ GE+GD G PG  G+ G++G +G  G  G DG  GQ G  G  G          +G DG+ G+      PGQKG++  KG  G PG  G  G KG+ G PG    +G+ G PG KG+ G  G
Sbjct:  156 KGSPGQKGDKGEKGEKGSNG---SPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTP------------------------------GSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGP------KGDKGADGAKGANGTPGAKGDKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQPGQKGDKGDKGQG-------EKGQDGQPGQKGQDGQPGQKGDKGEKGDIGQPGQKGDKGDKGQDGQPGQ---KGQDGQPGQKGQDGQPG 639          

HSP 2 Score: 108.227 bits (269), Expect = 1.514e-23
Identity = 192/545 (35.23%), Postives = 244/545 (44.77%), Query Frame = 2
Query: 2531 PGIDGKEGSIGPKGDLGPRGQSGNPGPQ--GIRGARGQSGPTGELGSKGD------------------------RGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP------TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            PG +GK+G+ G KGD G +G  G+ G    G++G  G  GP GE G  GD                        +G+ G  G  GDKGE+G+ GS G PG  G  G  G  G+PG KG KG  G  G  G  G KG +G  G KG+ G  G  G+ GD G  G  G KG +G  G  GS+G  G  G+ G +G  G PGS GD G +                     G  G  G  G+KG KG+ GL GP       G PG  G+ G  G  GA GA+G PG KG KG+ G+ GP+G++G D            G +G  G  G+ G+ G +G +G  G  G PG KG+ G  GL GP G                       G  G+PG  G  G +G  G  G  G  G +GD+G          PG  G+ G++G +GP G                            +G DG  G  G KGE+   G KG+ GT G+ G KG+ G  G  G +G  G PG KG+ G  G IG  G+ G+
Sbjct:   79 PGTNGKDGTPGDKGDTGDKGDKGDTGAPGVGVKGDPGAQGPPGEKGKDGDKGAPGAKGSKGDDGKKGDKGSSGEKGEKGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGAD---------------------GAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGAD------------GAKGANGTPGAKGDKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGD---------------------KGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKG---------------------------DKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGD 542          

HSP 3 Score: 106.686 bits (265), Expect = 4.765e-23
Identity = 191/537 (35.57%), Postives = 245/537 (45.62%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLG------------PRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQG---PTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP------TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGE 4033
            +G PG +GP   PG+ G DG +G+ G KG  G             +G+ G+PG +G +G +G+ G  G  GSKGD+G+ G  G   P GDKG                                   G  G  G  G KG +G  G KG+ G  G  G+ GD G  G  G KG +G  G  GS+G  G  G+ G +G  G PGS GD G +G KG  G  G                     +KG KG+ GL GP       G PG  G+ G  G  GA GA+G PG KG KG+ G+ GP+G++G DG  G  G+ G KG            + G +G +G  G  G PG KG+ G  GL GP G  G  G  GA G  G  G  G  G  G+PG  G  G  G  G  GT    G++GD+G  G+ G  G +G +G  G  G  G +G  G               ++G DG  G  G KG++ L G KG  G  G  GSKG+ G  G  G  G++G+ G KG+ G  G  G  G+
Sbjct:  111 KGDPGAQGP---PGEKGKDGDKGAPGAKGSKGDDGKKGDKGSSGEKGEKGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKG---------------------------------ADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPG---------------------QKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGADGAKGANGTPGAKG------------DKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGT---PGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADG---------------AKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQ 560          

HSP 4 Score: 75.8702 bits (185), Expect = 2.065e-13
Identity = 173/415 (41.69%), Postives = 213/415 (51.33%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDG---EKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP------TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKG--------ELGPIGLPGPVGTXXXXXXXXXXXXXXX---XXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE 3673
            G PG++G +G  G+ G DG +G  G  G  G +G  G  G  G +GA G  G  G+ G+ G +G  G  G  GDKGE+G  G +G  G  G  G  G  G  G  G KG  G PG +G  GEKG+ G  G KG+ G  G +G    KGD G  G  G KG  G  G KG +G+ GL G +G +G  G PG+ GD G  G  G +G  G+ G  G  G  G  G  G  G+KG KG+ GL GP       G PG  G+ G  G  GA GA+G PG KG KGD G+ GP+G++G DG  G  G KG KG  G+PG++G  G  G  G  G+ G  G PG+KG        E G  G PG  G  G  G+ G  G  G     GQ GD G  G  G PG+ G  G  G  G  G+ G+
Sbjct:  226 GNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGADGAKGANGTPGAKGDKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQPGQKGDKGDKGQGEKGQDGQPGQKGQDGQPGQKGDKGEKGDIGQPGQKGDKGDKGQDGQPGQKGQDGQPGQKGQDGQPGQ 640          

HSP 5 Score: 60.8474 bits (146), Expect = 8.345e-9
Identity = 47/101 (46.53%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KGA G  G+ G KGD G  G  G  G  G KG KGE G+ GP+G +G  G KG  G  G  G +G+ G  G +G+ G PG  G+ G KG  GP G++G DG
Sbjct:  312 KGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGADGAKGANGTPGAKGDKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGDKGADG 412          

HSP 6 Score: 57.7658 bits (138), Expect = 6.344e-8
Identity = 51/115 (44.35%), Postives = 65/115 (56.52%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVG---MKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKD---GNKGDEGSVGPPGYAGNPGIKGSR------GPVGNQGRDG 2131
            GA+G  G+ GAKGD G  G  G +G  G    KG KGE G+ GP+G    +G  G KGD G +G  G +G D   G+KGD+G+ G  G  G PG KG +      GP G++G DG
Sbjct:  361 GAKGANGTPGAKGDKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADG 475          

HSP 7 Score: 56.9954 bits (136), Expect = 1.142e-7
Identity = 49/111 (44.14%), Postives = 62/111 (55.86%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKD------GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA+G  G+ G+KGD G  G  G  G  G KG KGE G+ GP+G    +G  G KGD G +G  G +G D      G+KG++G  GP G  G  G KG+ G  G +G  GEK
Sbjct:  268 GAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGADGAKGANGTPGAKGDKGEK 378          

HSP 8 Score: 56.9954 bits (136), Expect = 1.309e-7
Identity = 47/110 (42.73%), Postives = 59/110 (53.64%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK---------DGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KG +G +G  G KGD G  G  G  G  G KG KGE G  G +G +G  G KGD G +G  GP+G           G+KG++G+ G  G  G PG KG +G  G +G DG
Sbjct:  342 KGDKGEKGLDGPKGDKGADGAKGANGTPGAKGDKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADG 451          

HSP 9 Score: 56.225 bits (134), Expect = 1.809e-7
Identity = 46/109 (42.20%), Postives = 61/109 (55.96%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKD------GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG +G +G+ GAKG  G  G  G +GE G+ G KG+ G  G +G  G  G KGD G +G  G +G D      G+KG++G  GP G  G  G  G++G  G +G DG K
Sbjct:  321 KGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGADGAKGANGTPGAKGDKGEKGTDGAKGADGTPGAKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAK 429          

HSP 10 Score: 56.225 bits (134), Expect = 1.856e-7
Identity = 47/105 (44.76%), Postives = 59/105 (56.19%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA+G  G+ G+KGD G  G  G  G  G KG KGE G+ GP+G    +G  G KGD G +G  G +G DG  G +G  G  G  G  G KG+ G  G++G  GEK
Sbjct:  427 GAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEK 531          

HSP 11 Score: 56.225 bits (134), Expect = 2.277e-7
Identity = 48/116 (41.38%), Postives = 62/116 (53.45%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD------HGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDG---------NKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            GA+G  G+ G KGD       GP G  G  G  G KG KGE G  G +G +G  G KGD G +G  GP+G  G         +KG++G +G PG  G+ G KG  G  G +G+DG+
Sbjct:  445 GAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQ 560          

HSP 12 Score: 55.8398 bits (133), Expect = 2.376e-7
Identity = 48/118 (40.68%), Postives = 63/118 (53.39%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK---------DGNKGDEGSVGPPGYAGNPGIKGSR------GPVGNQGRDGEK 2137
            KG +G  G+ G+KGD G  G  G +G  G  G KG+ G  G +G +G  G KGD G +G  GP+G           G+KGD+G+ G  G  G PG KG +      GP G++G DG K
Sbjct:  246 KGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGADGAK 363          

HSP 13 Score: 55.8398 bits (133), Expect = 2.915e-7
Identity = 47/109 (43.12%), Postives = 63/109 (57.80%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKD---GNKGDEGSVGPPGYAGNPGIKGSR------GPVGNQGRDG 2131
            G++G +GS GAKG     G  G++G+ G KG KG  G +G +G  G  G KGD G +G  G +G D   G+KGD+G+ G  G  G PG KG +      GP G++G DG
Sbjct:  211 GSKGDKGSDGAKG---ADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADG 316          

HSP 14 Score: 54.299 bits (129), Expect = 7.067e-7
Identity = 45/101 (44.55%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 1838 RGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQG---PRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G +GS G KGD G  G+ G+ G  G KG KGE G  G   P+G +G  G KG  G  G  G +G DG KG +G+ G  G  G+ G KGS G  G++G +G
Sbjct:  153 KGEKGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANG 253          

HSP 15 Score: 54.299 bits (129), Expect = 8.168e-7
Identity = 47/109 (43.12%), Postives = 59/109 (54.13%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKD------GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG +G  G+ GAKGD G  G  G +G  G  G KG+ G  G +G +G  G KGD G +G  GP+G        G KGD+G  G  G  G  G  GS+G  G++G DG K
Sbjct:  405 KGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPK 513          

HSP 16 Score: 53.9138 bits (128), Expect = 1.168e-6
Identity = 45/108 (41.67%), Postives = 61/108 (56.48%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGI------QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +G +GS GAKGD G +G  G++G+ G KG  G  G       +G +G +G  G  G  G +G+ G +G DG KGD+G+ G PG  G+ G KG+ G  G  G  G K
Sbjct:  235 GDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSK 342          

HSP 17 Score: 53.1434 bits (126), Expect = 1.614e-6
Identity = 50/120 (41.67%), Postives = 61/120 (50.83%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD------HGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGI------------QGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA+G  G+ GAKGD       GP G  G  G  G KG KGE G  G +G +G  G KGD G             +G+ G +G DG KGD+G+ G PG  G+ G KG+ G  G  G  G K
Sbjct:  382 GAKGADGTPGAKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSK 501          

HSP 18 Score: 52.373 bits (124), Expect = 3.216e-6
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV---KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G  G+ G KG+ G  G  G  G  G KG KG+ G+ GP+G +G  G    KGD G +G+ G  G+ G+KG++G  G PG  G  G  G +G  G++G+ GEK
Sbjct:  471 KGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQPGQKGDKGDKGQ-GEK 575          

HSP 19 Score: 51.9878 bits (123), Expect = 4.598e-6
Identity = 45/105 (42.86%), Postives = 58/105 (55.24%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGV---KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G++G +G  GA G+ GP G  G  G  G  G    KG+ G  G +G +G  G KGD G +GE   +G DG KGD+G+ G PG  G+ G KG+ G  G  G  G K
Sbjct:  178 GSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGE---KGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSK 279          

HSP 20 Score: 51.9878 bits (123), Expect = 4.638e-6
Identity = 44/103 (42.72%), Postives = 55/103 (53.40%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD------HGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            GA+G  G+ G KGD       GP G  G  G  G KG KG+ G  G +G +G  G KGD G +G  GP+G  G  G +G+ G PG  G+ G KG+ G  G  G
Sbjct:  286 GAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAKGADGTPGSKGDKGEKGLDGPKGDKGADGAKGANGTPGAKGDKGEKGTDGAKGADG 388          

HSP 21 Score: 51.9878 bits (123), Expect = 4.677e-6
Identity = 45/105 (42.86%), Postives = 54/105 (51.43%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSV---GPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G++G  G  G KG  G  G  G  G  G KG KGE G  G +G +G  G KGD G  G  G  G  G KGD+G     GP G  G  G  G++G  G++G DG K
Sbjct:  229 GSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGDKGADGAK 333          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Celegans
Match: col-135 (COLlagen [Source:UniProtKB/TrEMBL;Acc:G3MU58])

HSP 1 Score: 98.9821 bits (245), Expect = 9.852e-21
Identity = 185/527 (35.10%), Postives = 233/527 (44.21%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLG------------PRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQG---PTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGD---IGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            +G PG +GP   PG+ G DG +G+ G KG  G             +G+ G+PG +G +G +G+ G  G  GSKGD+G+ G  G   P GDKG                                   G  G  G  G KG +G  G KG+ G  G  G+ GD G  G  G KG +G  G  GS+G  G  G+ G +G  G PGS GD G +G KG  G  G +G  G  G+ G  G  G  G  G KGD    G  G  GA G  G  G  G  GA GA+G PG KG KG+ G+ GP+G++G D                  G  G+ G+ G +G +G  G  G PG KG+ G  GL GP G                       G  G+PG  G  G +G  G  G +G+ GE+G  G PG  G  GQ G  G  G                    G  G  G  G  G+ G+PGQKG++  KG  G PG  G  G KG+ G PG    +G+ G PG KG+ G  G
Sbjct:  111 KGDPGAQGP---PGEKGKDGDKGAPGAKGSKGDDGKKGDKGSSGEKGEKGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKG---------------------------------ADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGAD------------------GTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGD---------------------KGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQPGQKGDKGDK----------------GQGEKGQDGQPGQKGQDGQPGQKGDKGEKGDIGQPGQKGDKGDKGQDGQPGQ---KGQDGQPGQKGQDGQPG 543          

HSP 2 Score: 97.8265 bits (242), Expect = 2.112e-20
Identity = 153/406 (37.68%), Postives = 193/406 (47.54%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------XEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGS-----------KGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G KGS G  G  GEKG+ G  G  G KG +GE G  G+ G  G  G+ G +G  G PGS GD G +G KG  G  GS+G TG  G +G  G  G +      G KG KG+ G  G  GA G  G  G  G  GA GA+G PG KG KG+ G+ GP+G++G DG  G  G KG KG  G  G  G+ G+ G +G +G  G  G PG+KG+ G  GL GP G                       G  G+PG  G  G +G  G  G  G  G +GD+G  G+ G  G +G +G  G                          G +G  G +G+PGQKG++  KG  G PG  G  G            +GE G  G  G +G+ G PG KG+ G  G IG  G+ G+
Sbjct:  154 GEKGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGD---------------------KGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSK------------------------GDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQPGQKGDKGDKGQGEKGQDGQPGQKGQDGQPGQKGDKGEKGDIGQPGQKGD 514          

HSP 3 Score: 83.1889 bits (204), Expect = 7.900e-16
Identity = 166/422 (39.34%), Postives = 204/422 (48.34%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGP---------TGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP------TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKG--------ELGPIGLPGPVGTXXXXXXXXXXXXXXX---XXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE 3673
            +G PG +G  G  G+ G +G   S G KGD G +G  GNPGP+G +GA G  G  G  GSKGD+G  G +G          TGDKGE+G  G++G  G  G                                G +G  G KG+ G  G DG  G  G  G  G KG  G  G KG +G+ GL G +G +G  G PG+ GD G  G  G +G  G+ G  G  G  G  G  G  G+KG KG+ GL GP       G PG  G+ G  G  GA GA+G PG KG KGD G+ GP+G++G DG  G  G KG KG  G+PG++G  G  G  G  G+ G  G PG+KG        E G  G PG  G  G  G+ G  G  G     GQ GD G  G  G PG+ G  G  G  G  G+ G+
Sbjct:  156 KGSPGQKGDKGEKGEKGSNG---SPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTP------------------------------GSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQPGQKGDKGDKGQGEKGQDGQPGQKGQDGQPGQKGDKGEKGDIGQPGQKGDKGDKGQDGQPGQKGQDGQPGQKGQDGQPGQ 544          

HSP 4 Score: 78.9518 bits (193), Expect = 1.779e-14
Identity = 142/414 (34.30%), Postives = 178/414 (43.00%), Query Frame = 2
Query: 2873 LGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRG------------EVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVK---GTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G KG  G  GP GEKG  G  G  G KG++G            E G KGS G+ G  G +G +G +G PGS GD G  G  G                             G KGD G  G  GA G  G  G  G  GA GA+G PG K   G KG+ G  G +G++G +G  G  G KG KG  G  G  G+ G+ G +G +G  G  G PG+KG+ G  GL GP G                       G  G+PG  G  G +G  G  G  G  G +GD+G          PG  G+ G++G +GP G                            +G DG  G  G KGE+   G KG+ GT G+ G KG+ G  G  G +G  G PG KG+ G  G IG  G+ G+
Sbjct:  108 VGVKGDPGAQGPPGEKGKDGDKGAPGAKGSKGDDGKKGDKGSSGEKGEKGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNP---------------------------GPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGD---------------------KGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKG---------------------------DKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGD 446          

HSP 5 Score: 75.0998 bits (183), Expect = 2.627e-13
Identity = 149/400 (37.25%), Postives = 191/400 (47.75%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGI------KGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GV G  G+ G+ G  G  G+KGD G  G  G       VG+KG  G  G  G +G  G  G PG+                      G  G  G  G  G  GEKG K         G+PG+ G+ G  G+ G++G+ G  G KG KG  G  GP+G++G DG  G  G+ G KG +G  G +G+ GN G +G  G+ G +G  G KG+ G  G PG  G                 G+ G DG  G+ G PG  G +G  G  G  G  G++GD+G  G+ G  G +G +G  G  G  G +G  G  G  G  G+ G KG+ G  G  G PGQKG++  KG+      KG+ GT GA G KGE G  G  G  G  G+ G KG+ GL GP G  G DG
Sbjct:   77 GVPGTNGKDGTPGDKGDTGDKGDKGDTGAPG-------VGVKGDPGAQGPPGEKGKDGDKGAPGA---------------------KGSKGDDGKKGDKGSSGEKGEK---------GSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGD---------------KGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADG 424          

HSP 6 Score: 60.4622 bits (145), Expect = 1.003e-8
Identity = 50/112 (44.64%), Postives = 64/112 (57.14%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKD---GNKGDEGSVGPPGYAGNPGIKGSR------GPVGNQGRDG 2131
            GA+G  G+ G+KGD G  G  G  G  G KG KGE G+ GP+G    +G  G KGD G +G  G +G D   G+KGD+G+ G  G  G PG KG +      GP G++G DG
Sbjct:  268 GAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADG 379          

HSP 7 Score: 56.6102 bits (135), Expect = 1.378e-7
Identity = 47/105 (44.76%), Postives = 59/105 (56.19%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA+G  G+ G+KGD G  G  G  G  G KG KGE G+ GP+G    +G  G KGD G +G  G +G DG  G +G  G  G  G  G KG+ G  G++G  GEK
Sbjct:  331 GAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEK 435          

HSP 8 Score: 56.225 bits (134), Expect = 1.707e-7
Identity = 48/116 (41.38%), Postives = 62/116 (53.45%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD------HGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDG---------NKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            GA+G  G+ G KGD       GP G  G  G  G KG KGE G  G +G +G  G KGD G +G  GP+G  G         +KG++G +G PG  G+ G KG  G  G +G+DG+
Sbjct:  349 GAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQ 464          

HSP 9 Score: 55.8398 bits (133), Expect = 2.709e-7
Identity = 47/109 (43.12%), Postives = 63/109 (57.80%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKD---GNKGDEGSVGPPGYAGNPGIKGSR------GPVGNQGRDG 2131
            G++G +GS GAKG     G  G++G+ G KG KG  G +G +G  G  G KGD G +G  G +G D   G+KGD+G+ G  G  G PG KG +      GP G++G DG
Sbjct:  211 GSKGDKGSDGAKG---ADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADG 316          

HSP 10 Score: 55.0694 bits (131), Expect = 4.119e-7
Identity = 45/110 (40.91%), Postives = 60/110 (54.55%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK---------DGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KG +G  G+ G+KGD G  G  G +G  G  G KG+ G  G +G +G  G KGD G +G  GP+G           G+KG++G+ G  G  G PG KG +G  G +G DG
Sbjct:  246 KGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADG 355          

HSP 11 Score: 54.6842 bits (130), Expect = 5.849e-7
Identity = 45/101 (44.55%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 1838 RGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQG---PRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G +GS G KGD G  G+ G+ G  G KG KGE G  G   P+G +G  G KG  G  G  G +G DG KG +G+ G  G  G+ G KGS G  G++G +G
Sbjct:  153 KGEKGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANG 253          

HSP 12 Score: 54.6842 bits (130), Expect = 6.210e-7
Identity = 47/109 (43.12%), Postives = 59/109 (54.13%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKD------GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG +G  G+ GAKGD G  G  G +G  G  G KG+ G  G +G +G  G KGD G +G  GP+G        G KGD+G  G  G  G  G  GS+G  G++G DG K
Sbjct:  309 KGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPK 417          

HSP 13 Score: 53.9138 bits (128), Expect = 1.037e-6
Identity = 45/108 (41.67%), Postives = 61/108 (56.48%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGI------QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +G +GS GAKGD G +G  G++G+ G KG  G  G       +G +G +G  G  G  G +G+ G +G DG KGD+G+ G PG  G+ G KG+ G  G  G  G K
Sbjct:  235 GDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSK 342          

HSP 14 Score: 52.7582 bits (125), Expect = 2.277e-6
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV---KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G  G+ G KG+ G  G  G  G  G KG KG+ G+ GP+G +G  G    KGD G +G+ G  G+ G+KG++G  G PG  G  G  G +G  G++G+ GEK
Sbjct:  375 KGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGDKGLDGPKGDKGADGTPGSKGDKGEKGDIGQPGQKGDKGEKGQDGQPGQKGQDGQPGQKGDKGDKGQ-GEK 479          

HSP 15 Score: 52.373 bits (124), Expect = 3.178e-6
Identity = 48/111 (43.24%), Postives = 56/111 (50.45%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD------HGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSV---GPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA+G  G+ G KGD       GP G  G  G  G KG KGE G  G +G +G  G KGD G  G  G  G  G KGD+G     GP G  G  G  G++G  G +G DG K
Sbjct:  286 GAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAK 396          

HSP 16 Score: 51.9878 bits (123), Expect = 3.868e-6
Identity = 45/105 (42.86%), Postives = 58/105 (55.24%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGV---KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G++G +G  GA G+ GP G  G  G  G  G    KG+ G  G +G +G  G KGD G +GE   +G DG KGD+G+ G PG  G+ G KG+ G  G  G  G K
Sbjct:  178 GSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGE---KGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSK 279          

HSP 17 Score: 51.2174 bits (121), Expect = 6.459e-6
Identity = 45/105 (42.86%), Postives = 53/105 (50.48%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSV---GPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G++G  G  G KG  G  G  G  G  G KG KGE G  G +G +G  G KGD G  G  G  G  G KGD+G     GP G  G  G  G++G  G +G DG K
Sbjct:  229 GSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEKGADGAKGADGTPGSKGDKGADGAKGADGTPGQKGDKGEKGLDGPKGDKGADGTPGAKGDKGEKGADGAK 333          

HSP 18 Score: 50.8322 bits (120), Expect = 8.937e-6
Identity = 45/109 (41.28%), Postives = 59/109 (54.13%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGV------KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG+ G +G  G KG+ G +G  G++G+ G KG       KG+ G  G +G +G  G KGD G  G  G  G  G+KGD G  G  G  G  G KG+ G  G++G  GEK
Sbjct:  156 KGSPGQKGDKGEKGEKGSNGSPGSKGDKGEKGADGNPGPKGDKGADGAKGADGTPGSKGDKGSDGAKGADGNPGSKGDTGDKGEKGSDGAKGDKGANGTPGSKGDKGEK 264          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Celegans
Match: emb-9 (Collagen alpha-1(IV) chain [Source:UniProtKB/Swiss-Prot;Acc:P17139])

HSP 1 Score: 68.9366 bits (167), Expect = 3.424e-11
Identity = 179/557 (32.14%), Postives = 219/557 (39.32%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGK--EGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGK---------TGLMGSRGPQGVSGEPGSPGDAG----VNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGV--------KGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELG-----IQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQG-------PAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGI--VXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERY---LKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GPPG  G +G PG+PG+ G   EG +  +G  G +G+SG     G+ G  G SG  G  G+KGD G +G  G  G  G +G  G +          P                     +   G  G  G+ G KG  GP+GP G  G  GP G  GLKG +G++G  G  GK          G  GS+G QG+ G PG PG  G             +             G  G  G +G  GV+G  GL G  G PG +G  GP    GA GA GQPG+        KG +G+ G  G  GE GL                         G  G  G  GE G     IQGPPG  G+ G  G PG  G  G                         PG  G+ G  G       P G  GT GE G  G  G PG+ G  G RG  G +G   +  D                    +   G  G+VG PG  G+     L G  G PG  G  G+ G PG  G+ G  G  G PG +GE+GL+G  G  G DG
Sbjct:  139 GPPGQSGQNGNPGRPGLSGPPGEGGVNSQGRKGVKGESGR---SGVPGLPGNSGYPGLKGAKGDPGPYGLPGFPGVSGLKGRMGVRTSGVKGEKGLPGPPGPPGQPGSYPWASKPIEMEVLQGPVGPAGVKGEKGRDGPVGPPGMLGLDGPPGYPGLKGQKGDLGDAGQRGKRGKDGVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGEGTGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGP---VGAPGAPGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGL------------------------PGEPGDCGYPGEDGLPGYDIQGPPGLDGQSGRDGFPGIPGDIG------------------------DPGYSGEKGFPGTGVNKVGPDGYPGTPGERGMDGLPGFPGLHGEPGMRGQQGEVGFNGIDGDCGEPGLDGYPGAPGAPGAPGETGFGFPGQVGYPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDG 641          

HSP 2 Score: 63.1586 bits (152), Expect = 2.321e-9
Identity = 180/559 (32.20%), Postives = 217/559 (38.82%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGE-KGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEA-----------------GPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPP--------------GIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGK---------------RGNPGPKGETGLMGPIGPVGEDG 4039
            +GP G  G  G  G++G +GP G LG  G      P G  G +GQ G  G+ G +G RGK G  G  G+KG +                        G++G  G  G PG +G  GE G  G  G +G  GP GP GE  G+ GP G  G  G +G  G+ G +G  G +G RGP G  G PG PG  G+                                EKG +G+ G  G  G PG  GE                  GPPG  G SG +G PG+ G  GD G  G +G  G                                GV  ++G  G PG  GE G  GLPG  G                             G PG  G QG  G +G  G+ GE G  G P              G  G +G  GPNG  G  G  GP G PG  G  G  G PG  G  G DG  G+PG +GE  L GI G  G  G  G++G+ G PG +G  G                +G PG  G+ G  GP G  GEDG
Sbjct:  257 QGPVGPAGVKGEKGRDGPVGPPGMLGLDG------PPGYPGLKGQKGDLGDAGQRGKRGKDGVPGNYGEKGSQ------------------------GEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGEGTGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGPVGAPGAPGQPGIDGM----------------------------PGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPGYDIQGPPGLDGQSGRDGFPGIPGDIGDPGYSGEKGFPGT-------------------------------GVN-KVGPDGYPGTPGERGMDGLPGFPGLH---------------------------GEPGMRGQQGEVGFNGIDGDCGEPGLDGYPGAPGAPGAPGETGFGFPGQVGYPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDG 698          

HSP 3 Score: 61.6178 bits (148), Expect = 6.330e-9
Identity = 201/543 (37.02%), Postives = 235/543 (43.28%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGI------------------DGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGP-TGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGL--------KGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGD-IGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDT---GILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGP---PGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGER---------GDRGPPGIAGNMGQRGPN---GPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN 3979
            GPPG  G  G PG PG+                  DG  G+ G KG  G +G  G PG  G +G  G+ G  G  G +GD G  GP G  TG+ G  G  G  G                                     +G +GL G  G  GP+GP G  G  G  G+ G+        KG RGE G  G  G+ GL G  G  G  GE G PG       +G  G  G  G  G  G+ G  G PG  GEKG  G  +  +GP G PG  GE G  G  G  G  G+PG++G +G+    GI G  GE GLD             G    G  G  G  GP G  G  G+ GP   PGR G  G  G PG  G +G  G  G PG  G SG  G DG  G  G PG  G  G  G SG  G  G+          GD+G PG  G  G  GP+   G  G+VG  G RG+ G  G  G  G  G +GSRGLDG  G PG+ G   L G+ G+ G  G VG  GEPG PG  G  G+ GN
Sbjct:  276 GPPGMLGLDGPPGYPGLKGQKGDLGDAGQRGKRGKDGVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGEGTGEAGPHGAQGFDGV------------------------------------QGGKGLPGHDGLPGPVGPRGPVGAPGAPGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPG----YDIQGPPGLDGQSGRDGFPGIPGDIGDPGYSGEKGFPGTGVNKVGPDGYPGTPGERGMDGLPGFPGLHGEPGMRGQQGEVGFNGIDGDCGEPGLD-GYPGAPGAPGAPGETGFGFPGQVGYPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDGLVGFPGLRGEHGDNGLPGLEGECGEEGSRGLDGVPGYPGEHGTDGLPGLPGADGQPGFVGEAGEPGTPGYRGQPGEPGN 777          

HSP 4 Score: 59.6918 bits (143), Expect = 2.358e-8
Identity = 168/537 (31.28%), Postives = 201/537 (37.43%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGS---IGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKG---SEGKTGLMGSRGPQGVSGEPGSPGDAG------VNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGP-------AGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVG--EPGQKGERYLKGIKGSPGTIGAVGSKGEP-----------GPPGLTGPQGKRGNPGPKGETG 4003
            G PG  GP G PG PG+ G EG    +G KG  G RG  G+PG  G++G  G  G  GE G  G  G  G  G  G  G                                            G+ G  G  GR G  GP G    +G V   G+ G+KG  G  G+ G   + G  GL G++G  G  G PG PG +G      V     +  K     P  P      P    P   + ++G +G  G  G  GR G  GPPG  G  G  G PG+KG KGD G  G RG++G D                     G  GN G +G +GE G+ G PG  G  G  G PG  G                            PG  G  G +GP       AG  G QG  G +G +G PG  G  G  GP GP+G                       G  G  G+DG  G  E G +GE    G  G PG  G  G  G P           GPPGL G  G+ G PG  G+ G
Sbjct:   61 GAPGQDGPEGAPGAPGMFGAEGDFGDMGSKGARGDRGLPGSPGHPGLQGLDGLPGLKGEEGIPGCNGTDGFPGMPGLAGP------------------------------------------PGQSGQNGNPGRPGLSGPPG----EGGVNSQGRKGVKGESGRSGVPGLPGNSGYPGLKGAKGDPGPYGLPGFPGVSGLKGRMGVRTSGVKGEKGLPGPPGPPGQPGSYPWASKPIEMEVLQGPVGPAGVKGEKGRDGPVGPPGMLGLDGPPGYPGLKGQKGDLGDAGQRGKRGKD---------------------GVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGR---------------------------PGFEGDCGPEGPLGEGTGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGPVGAP---------------------GAPGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPGYDIQGPPGLDGQSGRDGFPGIPGDIG 482          

HSP 5 Score: 56.9954 bits (136), Expect = 1.585e-7
Identity = 127/401 (31.67%), Postives = 157/401 (39.15%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGL---PGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAG------LQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGE 4033
            G KG  G  G  GE G  G  G+ G +G  G  G+ G+EG  G MGS+G +G  G PGSPG  G+                                 +G+ G  GL G  G PG  G  G PG  G +G  GQ G  G  G  G+ GP GE G++                          SG  G+ G  G  G  G KG+ GP GL   PG  G  G  G   +              P      P  +       LQGP G +G +GE G  G  GPPG+   +G  GP G  G+                           +G  G +G+ GQ+G+R   G  G PG  G  GS+GE      PG PG  G  G+ G PG  G  G  GP GP+GE
Sbjct:   43 GTKGERGNPGFGGEPGHPGAPGQDGPEGAPGAPGMFGAEGDFGDMGSKGARGDRGLPGSPGHPGL---------------------------------QGLDGLPGLKGEEGIPGCNGTDGFPGMPGLAGPPGQSGQNGNPGRPGLSGPPGEGGVNSQGRKGVKGESG-------------RSGVPGLPGNSGYPGLKGAKGDPGPYGLPGFPGVSGLKGRMGVRTSGVKGEKGLPGPPGPPGQPGSYPWASKPIEMEVLQGPVGPAGVKGEKGRDGPVGPPGM---LGLDGPPGYPGL---------------------------KGQKGDLGDAGQRGKR---GKDGVPGNYGEKGSQGEQGLGGTPGYPGTKGGAGEPGYPGRPGFEGDCGPEGPLGE 364          

HSP 6 Score: 55.8398 bits (133), Expect = 3.972e-7
Identity = 189/530 (35.66%), Postives = 218/530 (41.13%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGD-IGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTG---ILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGP---PGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMG------QRGPNGPIGI------VXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN------PG------PK 3991
            PGT+G +G PG PG  G EG  GP+G LG    +G  GP G +G  G  G  G  G  G  G  GP+GP G  G                                             EKG RG  G  G  G  G  GE GD G  G+ GL G      I+G  G  G  G  G  G+ G+ G PG +G                                 EKG  G  +  +GP G PG  GE G  G  G  G  G+PG++G +G+ G   I G  GE GLDG  G  G+ G  G  G           GP G  G  G+ GP   PGR G  G  G PG  G +G  G  G PG  G SG  G DG  G  G PG  G  G  G SG  G  G+ G  G PG  G+ G      Q G  GP GI      VG  G RG+ G  G  G  G  G +GSRGLDG  G PG+ G   L G+ G+ G  G VG  GEPG PG  G  G+ GN      PG      P 
Sbjct:  335 PGTKGGAGEPGYPGRPGFEGDCGPEGPLGEG--TGEAGPHGAQGFDGVQGGKGLPGHDGLPGPVGPRGPVGAPGA-------------------------------PGQPGIDGMPGYTEKGDRGEDGYPGFAGEPGLPGEPGDCGYPGEDGLPGYD----IQGPPGLDGQSGRDGFPGIPGDIGDPGYSG---------------------------------EKGFPGTGVNKVGPDGYPGTPGERGMDGLPGFPGLHGEPGMRGQQGEVGFNGIDGDCGEPGLDGYPGAPGAPGAPGETGFGFPGQVG-YPGPNGDAGAAGLPGPDGYPGRDGLPGTPGYPGEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDGLVGFPGLRGEHGDNGLPGLEGECGEEGSRGLDGVPGYPGEHGTDGLPGLPGADGQPGFVGEAGEPGTPGYRGQPGEPGNLAYPGQPGDVGYPGPD 793          

HSP 7 Score: 55.8398 bits (133), Expect = 3.972e-7
Identity = 159/406 (39.16%), Postives = 185/406 (45.57%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLI------------GPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G  G+ G+ G+ G  G  GE G VG  GK G  GT G  G  G  G +G  G+ G  G+ G PG+PGD G  G  G+ G  G  G  G  G+ G PG       +G  GD GL             G  G PG  GE G  G  G  GA+GQPG  G  G+ G  G RG+ G  G L   G  G  G  G  G  G  G  G  G+ GE G  G     G  G  G PG  G  G  G  G PG PG +G  G  G  G PGLPG+ G  G  G  G +GE GE G  G PG  G  GQ+G  G  G  G  G  G P                  GL G+ G+PG KGE  L G  G PG+ G +G+ G+ G PG  G  G  GN G  G+ GL G  G  G+ G
Sbjct:  608 GEAGMNGQDGAPGQPGSRGESGLVGIDGKKGRDGTPGTRGQDGGPGYSGEAGAPGQNGMDGYPGAPGDQGYPGSPGQDGYPGPSGIPGEDGLVGFPGL------RGEHGDNGLPGLEGECGEEGSRGLDGVPGYPGEHGTDGLPGLPGADGQPGFVGEAGEPGTPGYRGQPGEPGNLAYPGQPGDVGYPGPDGPPGLPGQDGLPGLNGERGDNGD-SYPGNPGLSGQPGDAGYDGLDGVPGPPGYPGITGMPGLKGESGLPGLPGRQGNDGIPGQPGLEGECGEDGFPGSPGQPGYPGQQGREGEKGYPGIPGENGLP------------------GLRGQDGQPGLKGENGLDGQPGYPGSAGQLGTPGDVGYPGAPGENGDNGNQGRDGQPGLRGESGQPGQPG 988          

HSP 8 Score: 53.1434 bits (126), Expect = 2.180e-6
Identity = 44/101 (43.56%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G+ G  G  G  G  GQ G  G+ G +G KG  GI G  G  G+ G  G  G++GE G  G+ G  G  G +G PG  G PG  G  G  GNQGRDG+
Sbjct:  874 GQPGLEGECGEDGFPGSPGQPGYPGQQGREGEKGYPGIPGENGLPGLRGQDGQPGLKGENGLDGQPGYPGSAGQLGTPGDVGYPGAPGENGDNGNQGRDGQ 974          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Celegans
Match: dpy-17 (DumPY: shorter than wild-type [Source:UniProtKB/TrEMBL;Acc:Q20778])

HSP 1 Score: 62.003 bits (149), Expect = 1.739e-9
Identity = 69/201 (34.33%), Postives = 85/201 (42.29%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGS-------------IGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPG 3049
            +G PG  GP G+PG PG+DG++                 P G  GP G  G PG +G+RGARGQ+   G  GS G  G  GP GP G  GE G  G  G                                         GL G KG+ G  G  G++G+ G  G TG+ G  GE G +G +G  G  G+ G  G  GEPG
Sbjct:  161 KGTPGHDGPDGIPGVPGVDGEDADDAKAQTQQYDGCFTCPAGPQGPPGSQGKPGARGMRGARGQAAMPGRDGSPGMPGSLGPIGPPGAAGEEGPTGEPGADVEHQI----------------------------------GLPGAKGTPGAPGESGDQGEQGDRGATGIAGPPGERGPQGEKGDDGPNGAAGSPGEEGEPG 327          

HSP 2 Score: 51.2174 bits (121), Expect = 4.357e-6
Identity = 43/108 (39.81%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPE-----GIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G RG RG +   G  G  G  G+ G +G  G  GE G  G  G +     G+ G KG  G  GE+G +G+ G++G  G  GPPG  G  G KG  GP G  G  GE+
Sbjct:  216 RGMRGARGQAAMPGRDGSPGMPGSLGPIGPPGAAGEEGPTGEPGADVEHQIGLPGAKGTPGAPGESGDQGEQGDRGATGIAGPPGERGPQGEKGDDGPNGAAGSPGEE 323          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Celegans
Match: let-2 (Collagen alpha-2(IV) chain [Source:UniProtKB/Swiss-Prot;Acc:P17140])

HSP 1 Score: 61.2326 bits (147), Expect = 7.581e-9
Identity = 158/420 (37.62%), Postives = 194/420 (46.19%), Query Frame = 2
Query: 2852 EKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVK------------GTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSP-----------GLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            ++G  GL G KG  GP   DG+KGD G  G+ GL+G +G  G+ G  G  G  G  G  G  G+PG  G  G+ G+ G QG  GS G  G  G  G+ G+ G  G+ G+ G  GL G  G  G +G++G PG +GA G +GQPGV             G  GD G+ G  G++G +G  G  G  GL G +G  G  G  G  G  G  G+ G+ G PG KG+ G  G PG  G  G SG+ G  G PG  GQ G                   G PG  G +G +G SG  G  G +G+ G PG  G  G  G NG  G                            RG DG  G PG+ G+  + G+KG  G  G  G  G PG PGL G  G  G PG KGE G  G  G  G  GE
Sbjct: 1013 DRGFNGLPGEKGEAGPAARDGQKGDAGLPGQPGLRGPQGPSGLPGVPGFKGETGLPG-YGQPGQPGEKGLPGIPGKAGRQGAPGSPGQDGLPGFPGMKGESGYPGQDGLPGRDGLPGVPGQKGDLGQSGQPGLSGAPGLDGQPGVPGIRGDKGQGGLPGIPGDRGMDGYPGQKGENGYPGQPGLPGLGGEKGFAGTPGFPGLKGSPGYPGQDGLPGIPGLKGDSGFPGQPGQEGLPGLSGEKGMGGLPGMPGQPGQSIAGPVGPPGAPGLQGKDGFPGLPGQKGESGLSGLPGAPGLKGESGMPGFPGAKGDLGANGIPG---------------------------KRGEDGLPGVPGRDGQPGIPGLKGEVGGAGLPGQPGFPGIPGLKGEGGLPGFPGAKGEAGFPGTPGVPGYAGE 1404          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Fly
Match: Col4a1 (gene:FBgn0000299 transcript:FBtr0079003)

HSP 1 Score: 86.6557 bits (213), Expect = 2.096e-16
Identity = 206/554 (37.18%), Postives = 246/554 (44.40%), Query Frame = 2
Query: 2495 PGTRGPSGLPG---KPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGS---------KGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR--------IGEAGPPGK---TGASGAEGQPGVKGTKGD---TGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLP-------GKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGE-----RYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            PG RG +GLPG   +PGI G++G +GP   +GP G +G PG  GI G RG+ G  GE GS         KGDRG  G  GP                                                   KG  G+ G  G  GP G  G  G  G  G TGL G  G VG +G  G  GLMG +G QG++G PG  G  G+ GEKG QG  G  GP G  G     GQ G  G  G++GD G  G  G PG          G AGPPG+    G SG +G+ G+ G KG+    G+ G  GE+G  G  G  G+ G+ G  G  G  G+ G  G +G +GE G+ G PG KGE GP+GL G  G                 G  G+ G  G PGLP       G  G QG  G +G +GE GERG  GP G+AG  G RG  GP G  G                          G+ G  G+ G +GE       +KG KG PG  G  G +G  G PGL G +G  G  G  G  GL GPIGP G  GE
Sbjct:  889 PGDRGDAGLPGVSGRPGIVGEKGDVGP---IGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGP---------------------------------------------------KGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGA---------------PGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGL------------------------NGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGE 1349          

HSP 2 Score: 84.7297 bits (208), Expect = 7.676e-16
Identity = 181/541 (33.46%), Postives = 222/541 (41.04%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGP---KGDLGPRGQSGNPGPQGIRG--ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP---------------------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSP-------GDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPG---QKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM 4009
            PG  GP GLPG     G++G  GP   +GD GP G  G PG +G+ G    G++GP GE G +G  G  G  G  G+KGE+G                                           +GV G  G KGSVG                      +G  G++GD G  G +GL G +GE G  G +G TG  G +G +G+ G+PG P       GD G  GE+G  G                        EKG +G+ GL GP G  G  G+ G  G  GASG  G PG KG       +GPRGE G  G                              ++GE G+ G PGR G  G IG PG +G                             GLPG AG  G  G  G  G AG +G+RG  G  G  GQ G  G  G+ G  GP+G  G  G  G  G  G KG RGL G  G PG   QKG+    G+ G+ G +GA G +G  GP G  G  G  G PG KGE G++
Sbjct: 1048 PGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGL------------------------------------------QGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTG------------------------EKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGD------IGPRGEIGYPGVT----------------------------IKGEKGLPGRPGRNGRQGLIGAPGLIGER---------------------------GLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGML 1461          

HSP 3 Score: 84.3445 bits (207), Expect = 1.122e-15
Identity = 178/518 (34.36%), Postives = 218/518 (42.08%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSG-PTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGT--RGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            PG RG  G PG  G+ G +G  GP   +G +G +G PGP+G RG RGQ G P      +GD+G  G +G TG+KGE+G+ G  GP G AG                               KG RGL G  G+ G  G  G KGD+GP G+ G  G   +GE G+ G  G+ G  G  G  G+ GE G PG AG                                 E G+ G  G IGP G+ G  G AG PG+ G  G  G PG+KG   DTG  G +GE+GL+                              G EG+ G +G    +G  GP GLPG VG  G +                        G PG  G  GP G  G +G  G +G  G  G  G  GQ+G  G +                   P G  G  G  G +G  GEPG+ GER L GI+G  G  G  G  GE G  G  GP+G RG PG +G  G +G IG  GE G
Sbjct: 1170 PGDRGDKGEPGLSGLPGLKGETGP---VGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGA------------------------------KGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAG---------------------------------EPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKG---DTGPQGFKGERGLN------------------------------GFEGQKGDKG---DRGLQGPSGLPGLVGQKGDT------------------------GYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGML------------------PPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPG 1543          

HSP 4 Score: 83.5741 bits (205), Expect = 1.558e-15
Identity = 185/539 (34.32%), Postives = 233/539 (43.23%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRG------PQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGA---SGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGT------------IGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            +G PG  G  G PG+ G  G+ G  G KG+ GP+G  G  GP+G+ G RG+ G  G +G  G+ GK G +G  G  G+                             G          GP+G  G+ GL G KG  GP GP G  G  G     G  G RG+ G+ G  G+ G++G +G      P GV+G PG PG  GV G                                G KG+ G  G  G PG  G+ G PG  G    +G  G PG +G  G  G+ G +G++G  G  G  G  G +G  G PG  G  G+ G  G  G+ G+ G PG KG  G  GL GP G  G + + G  G PG SG  GD GP G+PG PG+ GL G A   G  G  GE+GD+G  GI G  G  G  G                               +GL G  G+PG+KG     GI G+PG             +G  G +G+ G PGL+G  G +G  GP G  G  G  GP GE G
Sbjct:  764 KGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQ----------------------------PGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADG---SVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRG------------------------------RDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAV-HGRAGPPGEKGDQGRSGIDGRDGINGEKG------------------------------EQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERG 1210          

HSP 5 Score: 80.1073 bits (196), Expect = 1.920e-14
Identity = 192/555 (34.59%), Postives = 234/555 (42.16%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQS--GNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP---------------QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKG---TKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKG---ELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERG-----DRGPPGI---AGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGA------VGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G++G +G+PG PG+ G +G  G KG+ G  G S  G PG  G  GA GQ G  G  G+ G +G  G +G  G K                                     +    GP G+KG  GL G  G  G  GP GE+G  G  G  G+ G  G  G KG +G+TGL G+ G                +G  G PG+PG  GV G KG +G  G  GP G               E G KG+ GL G     G PGR G  G PG  G S  +G+PG  G    KGD G  G  GE+G                      +      G +G  G+ G+ GPPG  G   E G  GL G  G  GP G +G  G  G  G+ G+ G VG PG PGK GL+G  G +G  G  GE G       GPPG+    G  G RGP GPIG                             G DG VG PG +G+  L G+ G PG +G       +G  G  GPPG+ G  G RG  G KGE G  G +G  G  G+
Sbjct:  496 GSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGK------------------------------------CSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKG---------------EFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQS-IKGEPGFHGRDGAKGDKGSFGRSGEKG--------------EPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFP---------------------------GADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGD 957          

HSP 6 Score: 72.7886 bits (177), Expect = 3.420e-12
Identity = 200/562 (35.59%), Postives = 238/562 (42.35%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGI------------------------------RGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----XXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXX----------XXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXX--XSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYL---------KGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            PG +GP+G PG PGI+GK+G  G  G  G  G SG PGP+G                               RG  G +GP G  G KG+RG  GP G  G +GE G  G +G                   +              GP+G +G+KG  GL+GRKG  GP G  G  G  G  G  G  G RG  G  G  G TG  G RG  G++G PG+PG  G  G  G  GK G  GP GP G       P          G  G +G+ G  GL GP G  G+ G AG PG+ G    EG PG KG KG  G+ GP+G                  G             G QG +G+ GI G PG KG  G  G  G  G  G S       P               G+PG  G  G++G  GG  +   AG +GD+G  G+ G  G+ G  GP G  G  G RG  G  G  GP G  G  G  GL G  GEPG+     L         KG  G+PG  G  G KG  G PG+ GP+G+ G    KGE GL G
Sbjct:  148 PGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGN---EGPPGKKGEKGTAGLNGPKGS-------IGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGF---KGEKGLSG 696          

HSP 7 Score: 68.1662 bits (165), Expect = 7.221e-11
Identity = 213/590 (36.10%), Postives = 260/590 (44.07%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---PGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---------GDVGPLGKTGLKGTRGEVGIKGS-----------------------------------EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGI---LGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKA-----GLQGPAGGSGTQGEAGERGDRGPPGIA-------------GNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM--GPIGPVGEDG 4039
            G RG  G  G+PG+ G++G  GP+GD G  GQ G    PG  G RG +G  GP G  G KGDRG+ G  G  G+ G++G+PG  G  G  G  GP G  G      G      P         G +GL G  G  GP G +G+K         G+ GP GK G KGT G  G KGS                                    G  GL GS+G +G  G  G+PGD+ +       G  G+ G  G  G  G PG PG +G+ G+KGD+G        GP G  G  G  G PGK GA G  G+ G  G +G  GI    GP GE+G DG  G  G+ G  G              G +G  G  G +G  G KG  G  G+PGP G  G  G+ G  G PG  G  G  G  G PG+PG++     G  G  G  G +G  G  G++G PG               G  GQ G  GP G                 G  G  G  G +G  G  G PG +G R L G +G  G  GAVG  G PG  GL G  G+ G PGP+GE G+   GP+GP G +G
Sbjct:  295 GPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGA--PGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLG----RPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPG---------------EDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNG 863          

HSP 8 Score: 67.781 bits (164), Expect = 9.697e-11
Identity = 214/550 (38.91%), Postives = 258/550 (46.91%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGK---PGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKT--GLMGSRGPQGVSGEPGSPGDA----GVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNS-------GPQGVEGELGIQGPPGRKGELGPIGLP------GPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE---------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG  GP+GL G+   PG++G  G  G KGD GP GQ G+ G +G  G  GQ+G  G  G  G+ G  G  G  G  G+ G PG +G  G  GP G  G  GS G+KG          +G  GE G RG  G  G  G  G  GE+GD GP    G KG RGE G+KG +G +  G M   G  G+ GE G P  +      +   G +G  G +G  G VG +G PG  G  G  G KG+ GL G  G  GR G  GPPG TG  G  G+PG+ G  G+ G  G  G  G  G  G +G  G  GG                    GPQG+ G  G+ GP G  G+ G  GLP      GP G  G  G  G  GP G  G  G  GP G  G  G AGL     G G QG  G+ G  G PG+ G+ G+RG  G  G             +G  G  G  G  G +G  G+ G PGQKG+  +KG  G   +    G KG+         PG  G  GP G+RG PG +G  G+ G  GP GE GE
Sbjct:   91 RGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPY---GAKGPRGEHGLKGEKGASCYGPM-KPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLP----GYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGA-------PGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGE 625          

HSP 9 Score: 67.781 bits (164), Expect = 1.138e-10
Identity = 205/578 (35.47%), Postives = 244/578 (42.21%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---------------------QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL----------------GKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGV--------------KGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV---GSKGEPGPPGLTGPQGKRGNPGPKGETGL-----MGPIGPVGEDGE 4042
            RGPPG RG    PG+ G DG  G  GP G+ G  G++G PG                      +G  GA G  G  G  G++G  G  GP+G  G KGE+G  G+ G  G  G  G  G  G PG                  E G  G  G KG  G  G  GEKG+ G                  G+TG+ G  GE G  G  G TGL G+ GP    G PG  G  G+N                              G +G KG+ G +G  G PG+ G  G PG+ G  G  G+PG+              +G KGD G  GP G  G D               G  G  G  G++G  GV G  GI G  G  G +GP G+ GP G  G  G  G  G  G  G  G  G  G+ G  G  G  GP G +G  G  G+RG  G PG++G  G +G  G  G  GP G RG PG  G MG  G+ G  G+ G  G  G PG+KG +   G+ G PG  G     G KGEPGP GL G  G  G PG  GE GL      G  GP GE G+
Sbjct:  596 RGPPGERG---YPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKG-------------EPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGP---QGPPGVEGPRGLN------------------------------GPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGAD---------------GSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGD 1109          

HSP 10 Score: 59.3066 bits (142), Expect = 4.244e-8
Identity = 103/287 (35.89%), Postives = 128/287 (44.60%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP---------QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGP 3322
            G RG  GL G+PG+ G  G IGP G  G RG +G+PG     G  GA G  G TG  G KG+RG  G +G  GDKG+RG  G  G PG  G                              +KG  G  G  G+ GP+G  GE+G  GP G+ G  GT    G+ G +G+ G++   GP          G  GEPG PG+ G+                            G +GE+G KG+ GLIG TG  GR G  G  G+ G  G EG  G+ G KG+ G   P
Sbjct: 1321 GERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVG------------------------------QKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGT---PGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGL---------------------------IGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAP 1547          

HSP 11 Score: 56.9954 bits (136), Expect = 2.085e-7
Identity = 202/546 (37.00%), Postives = 247/546 (45.24%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRG--------LLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEG---KTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEA---------GPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGE---AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP--------GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G  GP GL G  G+ G +G  G KG  G  G+ GN GP G +G +G +G  G  GS G  G  GP GP G KG+                  +   G  G KG  G+ G PG +G  GE+G +G         LGR G+ G  G  G+KGD    G+ G  G +G++GIKG  G    +   G +G +G SG PG PG  G        G  G +G  G  G  GI GQ GP GEKG  G  GL G TG PG+             G  G  GA GA+G  G KG +G  GI GP+GE G  G  G  G+ G  G  G+ GR G  G  G Q ++GE G  G  G KG+ G  G  G  G  G    D    P  G+            G PG+ G+ GP G  G+ GE    G +G+ GP G  G  G RG NGP G                            +G  G VG PG  G+  L+GI   PG  G  G +GEP        GPPGL G QG++G+ GP G  G  G  G VG  G+
Sbjct:  413 GAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGD----------------AGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDA---GRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGAR------GPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPG-QSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNK-----------GEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRG---------------------------EKGNQGAVGVPGNPGKDGLRGI---PGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGD 891          

HSP 12 Score: 55.8398 bits (133), Expect = 4.374e-7
Identity = 50/107 (46.73%), Postives = 61/107 (57.01%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPS--GQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPG--IKGSRGPVGNQGRDGEK 2137
            KG RGIRG  G      P   G  G++GE G  G KGE G +G  GP G+AG KGD G+QG  G  G +G  G +G +GP G  G PG  IKG +G  G  GR+G +
Sbjct: 1206 KGERGIRGQPGLPATV-PDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQ 1311          

HSP 13 Score: 55.0694 bits (131), Expect = 8.726e-7
Identity = 119/377 (31.56%), Postives = 149/377 (39.52%), Query Frame = 2
Query: 2900 IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGS-------GTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGE---PGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGET 4000
            I   G +G  GPLG TGLKG  G  G++G  G  G  G  GP G  G+ G  G  G++                                 G  G  G+ GP G PG       PG  G  G +GQ G+ G +G +G+ GPRG  G  G  G  G    + G    G +G  G  G  G+ G  G  G  G +G+ GP G  GP G  G  G+ GA                  PG PG  G +G    S          G  G+ G +G PG+ G  G+ GP G                                GLDG+ GE   PG  G+R  +G  G PG+ G  G +GEPG  GL G  G++G PG  G T
Sbjct:   85 IAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLH---------------------------------GQAGVPGVQGPAGNPGA------PGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPM-----------KPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEG------------------------------DTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGAT 381          

HSP 14 Score: 54.6842 bits (130), Expect = 1.007e-6
Identity = 114/287 (39.72%), Postives = 132/287 (45.99%), Query Frame = 2
Query: 3167 EKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG--IVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSP---GTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            EKG +G  G +GPTG  G +G  G  G +G  G +G PG  G +GD G    RG  GL             G  G PG QG  GN G  G+ G+ G  G  G  G  G  G+PGP G +G  G  G  G P         G  G PG  G AGL GP    G  GE GERGD GP G  G  G+ G  G  G    GP  P           P  +   K +  + G  G+ GQKGE  L G KG P   G  G  G KGE G PG  G +G++GN GP G TG  G
Sbjct:   87 EKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGE---RGSPGLH------------GQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGP---QGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKG 355          

HSP 15 Score: 54.6842 bits (130), Expect = 1.105e-6
Identity = 51/115 (44.35%), Postives = 60/115 (52.17%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVG------------TRGEVGMKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G  G+ G  G KGD GP+G +G             RG+ G+ GV G  GI G +G   P G AGV G  G+ G  G RG+DG KG+ GS   PG  G PG KG RG  GN G  G
Sbjct:  857 GPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGS---PGLVGMPGNKGDRGAPGNDGPKG 968          

HSP 16 Score: 54.299 bits (129), Expect = 1.398e-6
Identity = 190/519 (36.61%), Postives = 228/519 (43.93%), Query Frame = 2
Query: 2495 PGTRGPSGLPG------KPGIDGKEGSIGPKGDLG---------PRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGK---FGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG--------IKGS---EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEG---ELGIQGPPGRKGELGP-----IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPG 3940
            PGT G +G PG      +PG  G++G +G KGD+G         P+G  G  G  GI G  G  GP GE G  G+RG     G  GP G+KGE G  G  G  G  G      ++     KG KG  G PG +G  G KG  GL G  G  G  G  GEKG  G  G  G  G  G  G        IKG     G+ G  G +G  G SGE G PG   ++  K                             KG  G  G+ GP G  G  GE G  G  G +G +G PGV+G +G   + GPRGE+G                           N G  GV G   + G++G PGR G+ GP     I  PGP+G  G +G  G  G  G +G  G  G  GS G PG  G  G  G SG  G  GE+GD GP G AG  G  G  G  G+ G DG +G+PG  G +G  GN G +G+ G DG  G  G  G    +G  G PG  GA G KG  G
Sbjct:  533 PGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKG-----------------------NKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRG---LNGPRGEKG---------------------------NQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATG 998          

HSP 17 Score: 53.5286 bits (127), Expect = 2.112e-6
Identity = 103/295 (34.92%), Postives = 126/295 (42.71%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKP-------GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPD---GEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLD 3343
            GP G RG  G PG P       G  G +G  G  G+ G +G+ G  GP G+ GA+G  G  G  G+ G  G  G +G  G +GE                                           GEKG+ G  GR G  G IG     GE+G  G  G+ GL G  G +G  GS+G+ GL GS G  G  G PG+PG  G                 GP G +G  G  G  G+KG KGD GL GP+G PG +G+    G TG  G  G  G  G  G+ G  GP+G  G D
Sbjct: 1204 GPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGE----------------------------------IGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDT---------------GPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQK---GDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRD 1446          

HSP 18 Score: 53.5286 bits (127), Expect = 2.607e-6
Identity = 48/116 (41.38%), Postives = 62/116 (53.45%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVG-----TRGEVGM---------KGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA G+ G  GAKGD GP G++G      +GE G+         +G+ G  G+ G RG  G+AG  G +G+ G  GP G  G +G  GS G PG  G PG  G +G  G QG  GE+
Sbjct: 1268 GASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGER 1383          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Fly
Match: Col4a1 (gene:FBgn0000299 transcript:FBtr0079002)

HSP 1 Score: 86.6557 bits (213), Expect = 2.096e-16
Identity = 206/554 (37.18%), Postives = 246/554 (44.40%), Query Frame = 2
Query: 2495 PGTRGPSGLPG---KPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGS---------KGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR--------IGEAGPPGK---TGASGAEGQPGVKGTKGD---TGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLP-------GKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGE-----RYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            PG RG +GLPG   +PGI G++G +GP   +GP G +G PG  GI G RG+ G  GE GS         KGDRG  G  GP                                                   KG  G+ G  G  GP G  G  G  G  G TGL G  G VG +G  G  GLMG +G QG++G PG  G  G+ GEKG QG  G  GP G  G     GQ G  G  G++GD G  G  G PG          G AGPPG+    G SG +G+ G+ G KG+    G+ G  GE+G  G  G  G+ G+ G  G  G  G+ G  G +G +GE G+ G PG KGE GP+GL G  G                 G  G+ G  G PGLP       G  G QG  G +G +GE GERG  GP G+AG  G RG  GP G  G                          G+ G  G+ G +GE       +KG KG PG  G  G +G  G PGL G +G  G  G  G  GL GPIGP G  GE
Sbjct:  889 PGDRGDAGLPGVSGRPGIVGEKGDVGP---IGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGP---------------------------------------------------KGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGA---------------PGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGL------------------------NGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGE 1349          

HSP 2 Score: 84.7297 bits (208), Expect = 7.676e-16
Identity = 181/541 (33.46%), Postives = 222/541 (41.04%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGP---KGDLGPRGQSGNPGPQGIRG--ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP---------------------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSP-------GDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPG---QKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM 4009
            PG  GP GLPG     G++G  GP   +GD GP G  G PG +G+ G    G++GP GE G +G  G  G  G  G+KGE+G                                           +GV G  G KGSVG                      +G  G++GD G  G +GL G +GE G  G +G TG  G +G +G+ G+PG P       GD G  GE+G  G                        EKG +G+ GL GP G  G  G+ G  G  GASG  G PG KG       +GPRGE G  G                              ++GE G+ G PGR G  G IG PG +G                             GLPG AG  G  G  G  G AG +G+RG  G  G  GQ G  G  G+ G  GP+G  G  G  G  G  G KG RGL G  G PG   QKG+    G+ G+ G +GA G +G  GP G  G  G  G PG KGE G++
Sbjct: 1048 PGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGL------------------------------------------QGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTG------------------------EKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGD------IGPRGEIGYPGVT----------------------------IKGEKGLPGRPGRNGRQGLIGAPGLIGER---------------------------GLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGML 1461          

HSP 3 Score: 84.3445 bits (207), Expect = 1.122e-15
Identity = 178/518 (34.36%), Postives = 218/518 (42.08%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSG-PTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGT--RGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            PG RG  G PG  G+ G +G  GP   +G +G +G PGP+G RG RGQ G P      +GD+G  G +G TG+KGE+G+ G  GP G AG                               KG RGL G  G+ G  G  G KGD+GP G+ G  G   +GE G+ G  G+ G  G  G  G+ GE G PG AG                                 E G+ G  G IGP G+ G  G AG PG+ G  G  G PG+KG   DTG  G +GE+GL+                              G EG+ G +G    +G  GP GLPG VG  G +                        G PG  G  GP G  G +G  G +G  G  G  G  GQ+G  G +                   P G  G  G  G +G  GEPG+ GER L GI+G  G  G  G  GE G  G  GP+G RG PG +G  G +G IG  GE G
Sbjct: 1170 PGDRGDKGEPGLSGLPGLKGETGP---VGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGA------------------------------KGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAG---------------------------------EPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKG---DTGPQGFKGERGLN------------------------------GFEGQKGDKG---DRGLQGPSGLPGLVGQKGDT------------------------GYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGML------------------PPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPG 1543          

HSP 4 Score: 83.5741 bits (205), Expect = 1.558e-15
Identity = 185/539 (34.32%), Postives = 233/539 (43.23%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRG------PQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGA---SGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGT------------IGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            +G PG  G  G PG+ G  G+ G  G KG+ GP+G  G  GP+G+ G RG+ G  G +G  G+ GK G +G  G  G+                             G          GP+G  G+ GL G KG  GP GP G  G  G     G  G RG+ G+ G  G+ G++G +G      P GV+G PG PG  GV G                                G KG+ G  G  G PG  G+ G PG  G    +G  G PG +G  G  G+ G +G++G  G  G  G  G +G  G PG  G  G+ G  G  G+ G+ G PG KG  G  GL GP G  G + + G  G PG SG  GD GP G+PG PG+ GL G A   G  G  GE+GD+G  GI G  G  G  G                               +GL G  G+PG+KG     GI G+PG             +G  G +G+ G PGL+G  G +G  GP G  G  G  GP GE G
Sbjct:  764 KGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQ----------------------------PGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADG---SVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRG------------------------------RDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAV-HGRAGPPGEKGDQGRSGIDGRDGINGEKG------------------------------EQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERG 1210          

HSP 5 Score: 80.1073 bits (196), Expect = 1.920e-14
Identity = 192/555 (34.59%), Postives = 234/555 (42.16%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQS--GNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP---------------QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKG---TKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKG---ELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERG-----DRGPPGI---AGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGA------VGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G++G +G+PG PG+ G +G  G KG+ G  G S  G PG  G  GA GQ G  G  G+ G +G  G +G  G K                                     +    GP G+KG  GL G  G  G  GP GE+G  G  G  G+ G  G  G KG +G+TGL G+ G                +G  G PG+PG  GV G KG +G  G  GP G               E G KG+ GL G     G PGR G  G PG  G S  +G+PG  G    KGD G  G  GE+G                      +      G +G  G+ G+ GPPG  G   E G  GL G  G  GP G +G  G  G  G+ G+ G VG PG PGK GL+G  G +G  G  GE G       GPPG+    G  G RGP GPIG                             G DG VG PG +G+  L G+ G PG +G       +G  G  GPPG+ G  G RG  G KGE G  G +G  G  G+
Sbjct:  496 GSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGK------------------------------------CSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKG---------------EFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQS-IKGEPGFHGRDGAKGDKGSFGRSGEKG--------------EPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFP---------------------------GADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGD 957          

HSP 6 Score: 72.7886 bits (177), Expect = 3.420e-12
Identity = 200/562 (35.59%), Postives = 238/562 (42.35%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGI------------------------------RGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----XXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXX----------XXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXX--XSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYL---------KGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            PG +GP+G PG PGI+GK+G  G  G  G  G SG PGP+G                               RG  G +GP G  G KG+RG  GP G  G +GE G  G +G                   +              GP+G +G+KG  GL+GRKG  GP G  G  G  G  G  G  G RG  G  G  G TG  G RG  G++G PG+PG  G  G  G  GK G  GP GP G       P          G  G +G+ G  GL GP G  G+ G AG PG+ G    EG PG KG KG  G+ GP+G                  G             G QG +G+ GI G PG KG  G  G  G  G  G S       P               G+PG  G  G++G  GG  +   AG +GD+G  G+ G  G+ G  GP G  G  G RG  G  G  GP G  G  G  GL G  GEPG+     L         KG  G+PG  G  G KG  G PG+ GP+G+ G    KGE GL G
Sbjct:  148 PGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGN---EGPPGKKGEKGTAGLNGPKGS-------IGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGF---KGEKGLSG 696          

HSP 7 Score: 68.1662 bits (165), Expect = 7.221e-11
Identity = 213/590 (36.10%), Postives = 260/590 (44.07%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---PGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---------GDVGPLGKTGLKGTRGEVGIKGS-----------------------------------EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGI---LGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKA-----GLQGPAGGSGTQGEAGERGDRGPPGIA-------------GNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM--GPIGPVGEDG 4039
            G RG  G  G+PG+ G++G  GP+GD G  GQ G    PG  G RG +G  GP G  G KGDRG+ G  G  G+ G++G+PG  G  G  G  GP G  G      G      P         G +GL G  G  GP G +G+K         G+ GP GK G KGT G  G KGS                                    G  GL GS+G +G  G  G+PGD+ +       G  G+ G  G  G  G PG PG +G+ G+KGD+G        GP G  G  G  G PGK GA G  G+ G  G +G  GI    GP GE+G DG  G  G+ G  G              G +G  G  G +G  G KG  G  G+PGP G  G  G+ G  G PG  G  G  G  G PG+PG++     G  G  G  G +G  G  G++G PG               G  GQ G  GP G                 G  G  G  G +G  G  G PG +G R L G +G  G  GAVG  G PG  GL G  G+ G PGP+GE G+   GP+GP G +G
Sbjct:  295 GPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGA--PGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLG----RPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPG---------------EDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNG 863          

HSP 8 Score: 67.781 bits (164), Expect = 9.697e-11
Identity = 214/550 (38.91%), Postives = 258/550 (46.91%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGK---PGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKT--GLMGSRGPQGVSGEPGSPGDA----GVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNS-------GPQGVEGELGIQGPPGRKGELGPIGLP------GPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE---------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG  GP+GL G+   PG++G  G  G KGD GP GQ G+ G +G  G  GQ+G  G  G  G+ G  G  G  G  G+ G PG +G  G  GP G  G  GS G+KG          +G  GE G RG  G  G  G  G  GE+GD GP    G KG RGE G+KG +G +  G M   G  G+ GE G P  +      +   G +G  G +G  G VG +G PG  G  G  G KG+ GL G  G  GR G  GPPG TG  G  G+PG+ G  G+ G  G  G  G  G  G +G  G  GG                    GPQG+ G  G+ GP G  G+ G  GLP      GP G  G  G  G  GP G  G  G  GP G  G  G AGL     G G QG  G+ G  G PG+ G+ G+RG  G  G             +G  G  G  G  G +G  G+ G PGQKG+  +KG  G   +    G KG+         PG  G  GP G+RG PG +G  G+ G  GP GE GE
Sbjct:   91 RGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPY---GAKGPRGEHGLKGEKGASCYGPM-KPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLP----GYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGA-------PGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGE 625          

HSP 9 Score: 67.781 bits (164), Expect = 1.138e-10
Identity = 205/578 (35.47%), Postives = 244/578 (42.21%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---------------------QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL----------------GKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGV--------------KGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV---GSKGEPGPPGLTGPQGKRGNPGPKGETGL-----MGPIGPVGEDGE 4042
            RGPPG RG    PG+ G DG  G  GP G+ G  G++G PG                      +G  GA G  G  G  G++G  G  GP+G  G KGE+G  G+ G  G  G  G  G  G PG                  E G  G  G KG  G  G  GEKG+ G                  G+TG+ G  GE G  G  G TGL G+ GP    G PG  G  G+N                              G +G KG+ G +G  G PG+ G  G PG+ G  G  G+PG+              +G KGD G  GP G  G D               G  G  G  G++G  GV G  GI G  G  G +GP G+ GP G  G  G  G  G  G  G  G  G  G+ G  G  G  GP G +G  G  G+RG  G PG++G  G +G  G  G  GP G RG PG  G MG  G+ G  G+ G  G  G PG+KG +   G+ G PG  G     G KGEPGP GL G  G  G PG  GE GL      G  GP GE G+
Sbjct:  596 RGPPGERG---YPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKG-------------EPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGP---QGPPGVEGPRGLN------------------------------GPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGAD---------------GSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGD 1109          

HSP 10 Score: 59.3066 bits (142), Expect = 4.244e-8
Identity = 103/287 (35.89%), Postives = 128/287 (44.60%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP---------QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGP 3322
            G RG  GL G+PG+ G  G IGP G  G RG +G+PG     G  GA G  G TG  G KG+RG  G +G  GDKG+RG  G  G PG  G                              +KG  G  G  G+ GP+G  GE+G  GP G+ G  GT    G+ G +G+ G++   GP          G  GEPG PG+ G+                            G +GE+G KG+ GLIG TG  GR G  G  G+ G  G EG  G+ G KG+ G   P
Sbjct: 1321 GERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVG------------------------------QKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGT---PGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGL---------------------------IGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAP 1547          

HSP 11 Score: 56.9954 bits (136), Expect = 2.085e-7
Identity = 202/546 (37.00%), Postives = 247/546 (45.24%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRG--------LLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEG---KTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEA---------GPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGE---AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP--------GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G  GP GL G  G+ G +G  G KG  G  G+ GN GP G +G +G +G  G  GS G  G  GP GP G KG+                  +   G  G KG  G+ G PG +G  GE+G +G         LGR G+ G  G  G+KGD    G+ G  G +G++GIKG  G    +   G +G +G SG PG PG  G        G  G +G  G  G  GI GQ GP GEKG  G  GL G TG PG+             G  G  GA GA+G  G KG +G  GI GP+GE G  G  G  G+ G  G  G+ GR G  G  G Q ++GE G  G  G KG+ G  G  G  G  G    D    P  G+            G PG+ G+ GP G  G+ GE    G +G+ GP G  G  G RG NGP G                            +G  G VG PG  G+  L+GI   PG  G  G +GEP        GPPGL G QG++G+ GP G  G  G  G VG  G+
Sbjct:  413 GAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGD----------------AGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDA---GRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGAR------GPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPG-QSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNK-----------GEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRG---------------------------EKGNQGAVGVPGNPGKDGLRGI---PGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGD 891          

HSP 12 Score: 55.8398 bits (133), Expect = 4.374e-7
Identity = 50/107 (46.73%), Postives = 61/107 (57.01%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPS--GQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPG--IKGSRGPVGNQGRDGEK 2137
            KG RGIRG  G      P   G  G++GE G  G KGE G +G  GP G+AG KGD G+QG  G  G +G  G +G +GP G  G PG  IKG +G  G  GR+G +
Sbjct: 1206 KGERGIRGQPGLPATV-PDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQ 1311          

HSP 13 Score: 55.0694 bits (131), Expect = 8.726e-7
Identity = 119/377 (31.56%), Postives = 149/377 (39.52%), Query Frame = 2
Query: 2900 IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGS-------GTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGE---PGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGET 4000
            I   G +G  GPLG TGLKG  G  G++G  G  G  G  GP G  G+ G  G  G++                                 G  G  G+ GP G PG       PG  G  G +GQ G+ G +G +G+ GPRG  G  G  G  G    + G    G +G  G  G  G+ G  G  G  G +G+ GP G  GP G  G  G+ GA                  PG PG  G +G    S          G  G+ G +G PG+ G  G+ GP G                                GLDG+ GE   PG  G+R  +G  G PG+ G  G +GEPG  GL G  G++G PG  G T
Sbjct:   85 IAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLH---------------------------------GQAGVPGVQGPAGNPGA------PGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPM-----------KPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEG------------------------------DTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGAT 381          

HSP 14 Score: 54.6842 bits (130), Expect = 1.007e-6
Identity = 114/287 (39.72%), Postives = 132/287 (45.99%), Query Frame = 2
Query: 3167 EKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG--IVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSP---GTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            EKG +G  G +GPTG  G +G  G  G +G  G +G PG  G +GD G    RG  GL             G  G PG QG  GN G  G+ G+ G  G  G  G  G  G+PGP G +G  G  G  G P         G  G PG  G AGL GP    G  GE GERGD GP G  G  G+ G  G  G    GP  P           P  +   K +  + G  G+ GQKGE  L G KG P   G  G  G KGE G PG  G +G++GN GP G TG  G
Sbjct:   87 EKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGE---RGSPGLH------------GQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGP---QGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKG 355          

HSP 15 Score: 54.6842 bits (130), Expect = 1.105e-6
Identity = 51/115 (44.35%), Postives = 60/115 (52.17%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVG------------TRGEVGMKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G  G+ G  G KGD GP+G +G             RG+ G+ GV G  GI G +G   P G AGV G  G+ G  G RG+DG KG+ GS   PG  G PG KG RG  GN G  G
Sbjct:  857 GPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGS---PGLVGMPGNKGDRGAPGNDGPKG 968          

HSP 16 Score: 54.299 bits (129), Expect = 1.398e-6
Identity = 190/519 (36.61%), Postives = 228/519 (43.93%), Query Frame = 2
Query: 2495 PGTRGPSGLPG------KPGIDGKEGSIGPKGDLG---------PRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGK---FGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG--------IKGS---EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEG---ELGIQGPPGRKGELGP-----IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPG 3940
            PGT G +G PG      +PG  G++G +G KGD+G         P+G  G  G  GI G  G  GP GE G  G+RG     G  GP G+KGE G  G  G  G  G      ++     KG KG  G PG +G  G KG  GL G  G  G  G  GEKG  G  G  G  G  G  G        IKG     G+ G  G +G  G SGE G PG   ++  K                             KG  G  G+ GP G  G  GE G  G  G +G +G PGV+G +G   + GPRGE+G                           N G  GV G   + G++G PGR G+ GP     I  PGP+G  G +G  G  G  G +G  G  G  GS G PG  G  G  G SG  G  GE+GD GP G AG  G  G  G  G+ G DG +G+PG  G +G  GN G +G+ G DG  G  G  G    +G  G PG  GA G KG  G
Sbjct:  533 PGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKG-----------------------NKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRG---LNGPRGEKG---------------------------NQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATG 998          

HSP 17 Score: 53.5286 bits (127), Expect = 2.112e-6
Identity = 103/295 (34.92%), Postives = 126/295 (42.71%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKP-------GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPD---GEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLD 3343
            GP G RG  G PG P       G  G +G  G  G+ G +G+ G  GP G+ GA+G  G  G  G+ G  G  G +G  G +GE                                           GEKG+ G  GR G  G IG     GE+G  G  G+ GL G  G +G  GS+G+ GL GS G  G  G PG+PG  G                 GP G +G  G  G  G+KG KGD GL GP+G PG +G+    G TG  G  G  G  G  G+ G  GP+G  G D
Sbjct: 1204 GPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGE----------------------------------IGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDT---------------GPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQK---GDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRD 1446          

HSP 18 Score: 53.5286 bits (127), Expect = 2.607e-6
Identity = 48/116 (41.38%), Postives = 62/116 (53.45%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVG-----TRGEVGM---------KGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA G+ G  GAKGD GP G++G      +GE G+         +G+ G  G+ G RG  G+AG  G +G+ G  GP G  G +G  GS G PG  G PG  G +G  G QG  GE+
Sbjct: 1268 GASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGER 1383          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Fly
Match: Col4a1 (gene:FBgn0000299 transcript:FBtr0079001)

HSP 1 Score: 86.6557 bits (213), Expect = 2.096e-16
Identity = 206/554 (37.18%), Postives = 246/554 (44.40%), Query Frame = 2
Query: 2495 PGTRGPSGLPG---KPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGS---------KGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR--------IGEAGPPGK---TGASGAEGQPGVKGTKGD---TGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLP-------GKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGE-----RYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            PG RG +GLPG   +PGI G++G +GP   +GP G +G PG  GI G RG+ G  GE GS         KGDRG  G  GP                                                   KG  G+ G  G  GP G  G  G  G  G TGL G  G VG +G  G  GLMG +G QG++G PG  G  G+ GEKG QG  G  GP G  G     GQ G  G  G++GD G  G  G PG          G AGPPG+    G SG +G+ G+ G KG+    G+ G  GE+G  G  G  G+ G+ G  G  G  G+ G  G +G +GE G+ G PG KGE GP+GL G  G                 G  G+ G  G PGLP       G  G QG  G +G +GE GERG  GP G+AG  G RG  GP G  G                          G+ G  G+ G +GE       +KG KG PG  G  G +G  G PGL G +G  G  G  G  GL GPIGP G  GE
Sbjct:  889 PGDRGDAGLPGVSGRPGIVGEKGDVGP---IGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGP---------------------------------------------------KGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGA---------------PGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGL------------------------NGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGE 1349          

HSP 2 Score: 84.7297 bits (208), Expect = 7.676e-16
Identity = 181/541 (33.46%), Postives = 222/541 (41.04%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGP---KGDLGPRGQSGNPGPQGIRG--ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP---------------------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSP-------GDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPG---QKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM 4009
            PG  GP GLPG     G++G  GP   +GD GP G  G PG +G+ G    G++GP GE G +G  G  G  G  G+KGE+G                                           +GV G  G KGSVG                      +G  G++GD G  G +GL G +GE G  G +G TG  G +G +G+ G+PG P       GD G  GE+G  G                        EKG +G+ GL GP G  G  G+ G  G  GASG  G PG KG       +GPRGE G  G                              ++GE G+ G PGR G  G IG PG +G                             GLPG AG  G  G  G  G AG +G+RG  G  G  GQ G  G  G+ G  GP+G  G  G  G  G  G KG RGL G  G PG   QKG+    G+ G+ G +GA G +G  GP G  G  G  G PG KGE G++
Sbjct: 1048 PGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGDQGRSGIDGRDGINGEKGEQGL------------------------------------------QGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTG------------------------EKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGD------IGPRGEIGYPGVT----------------------------IKGEKGLPGRPGRNGRQGLIGAPGLIGER---------------------------GLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGML 1461          

HSP 3 Score: 84.3445 bits (207), Expect = 1.122e-15
Identity = 178/518 (34.36%), Postives = 218/518 (42.08%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSG-PTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGT--RGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            PG RG  G PG  G+ G +G  GP   +G +G +G PGP+G RG RGQ G P      +GD+G  G +G TG+KGE+G+ G  GP G AG                               KG RGL G  G+ G  G  G KGD+GP G+ G  G   +GE G+ G  G+ G  G  G  G+ GE G PG AG                                 E G+ G  G IGP G+ G  G AG PG+ G  G  G PG+KG   DTG  G +GE+GL+                              G EG+ G +G    +G  GP GLPG VG  G +                        G PG  G  GP G  G +G  G +G  G  G  G  GQ+G  G +                   P G  G  G  G +G  GEPG+ GER L GI+G  G  G  G  GE G  G  GP+G RG PG +G  G +G IG  GE G
Sbjct: 1170 PGDRGDKGEPGLSGLPGLKGETGP---VGLQGFTGAPGPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGA------------------------------KGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAG---------------------------------EPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKG---DTGPQGFKGERGLN------------------------------GFEGQKGDKG---DRGLQGPSGLPGLVGQKGDT------------------------GYPGLNGNDGPVGAPGERGFTGPKGRDGRDGTPGLPGQKGEPGML------------------PPPGPKGEPGQPGRNGPKGEPGRPGERGLIGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPG 1543          

HSP 4 Score: 83.5741 bits (205), Expect = 1.558e-15
Identity = 185/539 (34.32%), Postives = 233/539 (43.23%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRG------PQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGA---SGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGT------------IGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            +G PG  G  G PG+ G  G+ G  G KG+ GP+G  G  GP+G+ G RG+ G  G +G  G+ GK G +G  G  G+                             G          GP+G  G+ GL G KG  GP GP G  G  G     G  G RG+ G+ G  G+ G++G +G      P GV+G PG PG  GV G                                G KG+ G  G  G PG  G+ G PG  G    +G  G PG +G  G  G+ G +G++G  G  G  G  G +G  G PG  G  G+ G  G  G+ G+ G PG KG  G  GL GP G  G + + G  G PG SG  GD GP G+PG PG+ GL G A   G  G  GE+GD+G  GI G  G  G  G                               +GL G  G+PG+KG     GI G+PG             +G  G +G+ G PGL+G  G +G  GP G  G  G  GP GE G
Sbjct:  764 KGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQ----------------------------PGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADG---SVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRG------------------------------RDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAV-HGRAGPPGEKGDQGRSGIDGRDGINGEKG------------------------------EQGLQGVWGQPGEKGSVGAPGIPGAPGMDGLPGAAGAPGAVGYPGDRGDKGEPGLSGLPGLKGETGPVGLQGFTGAPGPKGERG 1210          

HSP 5 Score: 80.1073 bits (196), Expect = 1.920e-14
Identity = 192/555 (34.59%), Postives = 234/555 (42.16%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQS--GNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP---------------QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKG---TKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKG---ELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERG-----DRGPPGI---AGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGA------VGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G++G +G+PG PG+ G +G  G KG+ G  G S  G PG  G  GA GQ G  G  G+ G +G  G +G  G K                                     +    GP G+KG  GL G  G  G  GP GE+G  G  G  G+ G  G  G KG +G+TGL G+ G                +G  G PG+PG  GV G KG +G  G  GP G               E G KG+ GL G     G PGR G  G PG  G S  +G+PG  G    KGD G  G  GE+G                      +      G +G  G+ G+ GPPG  G   E G  GL G  G  GP G +G  G  G  G+ G+ G VG PG PGK GL+G  G +G  G  GE G       GPPG+    G  G RGP GPIG                             G DG VG PG +G+  L G+ G PG +G       +G  G  GPPG+ G  G RG  G KGE G  G +G  G  G+
Sbjct:  496 GSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGK------------------------------------CSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKG---------------EFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQS-IKGEPGFHGRDGAKGDKGSFGRSGEKG--------------EPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFP---------------------------GADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGD 957          

HSP 6 Score: 72.7886 bits (177), Expect = 3.420e-12
Identity = 200/562 (35.59%), Postives = 238/562 (42.35%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGI------------------------------RGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----XXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXX----------XXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXX--XSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYL---------KGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            PG +GP+G PG PGI+GK+G  G  G  G  G SG PGP+G                               RG  G +GP G  G KG+RG  GP G  G +GE G  G +G                   +              GP+G +G+KG  GL+GRKG  GP G  G  G  G  G  G  G RG  G  G  G TG  G RG  G++G PG+PG  G  G  G  GK G  GP GP G       P          G  G +G+ G  GL GP G  G+ G AG PG+ G    EG PG KG KG  G+ GP+G                  G             G QG +G+ GI G PG KG  G  G  G  G  G S       P               G+PG  G  G++G  GG  +   AG +GD+G  G+ G  G+ G  GP G  G  G RG  G  G  GP G  G  G  GL G  GEPG+     L         KG  G+PG  G  G KG  G PG+ GP+G+ G    KGE GL G
Sbjct:  148 PGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGN---EGPPGKKGEKGTAGLNGPKGS-------IGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGF---KGEKGLSG 696          

HSP 7 Score: 68.1662 bits (165), Expect = 7.221e-11
Identity = 213/590 (36.10%), Postives = 260/590 (44.07%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---PGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---------GDVGPLGKTGLKGTRGEVGIKGS-----------------------------------EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGI---LGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKA-----GLQGPAGGSGTQGEAGERGDRGPPGIA-------------GNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM--GPIGPVGEDG 4039
            G RG  G  G+PG+ G++G  GP+GD G  GQ G    PG  G RG +G  GP G  G KGDRG+ G  G  G+ G++G+PG  G  G  G  GP G  G      G      P         G +GL G  G  GP G +G+K         G+ GP GK G KGT G  G KGS                                    G  GL GS+G +G  G  G+PGD+ +       G  G+ G  G  G  G PG PG +G+ G+KGD+G        GP G  G  G  G PGK GA G  G+ G  G +G  GI    GP GE+G DG  G  G+ G  G              G +G  G  G +G  G KG  G  G+PGP G  G  G+ G  G PG  G  G  G  G PG+PG++     G  G  G  G +G  G  G++G PG               G  GQ G  GP G                 G  G  G  G +G  G  G PG +G R L G +G  G  GAVG  G PG  GL G  G+ G PGP+GE G+   GP+GP G +G
Sbjct:  295 GPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGA--PGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLG----RPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPG---------------EDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNG 863          

HSP 8 Score: 67.781 bits (164), Expect = 9.697e-11
Identity = 214/550 (38.91%), Postives = 258/550 (46.91%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGK---PGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKT--GLMGSRGPQGVSGEPGSPGDA----GVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNS-------GPQGVEGELGIQGPPGRKGELGPIGLP------GPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE---------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG  GP+GL G+   PG++G  G  G KGD GP GQ G+ G +G  G  GQ+G  G  G  G+ G  G  G  G  G+ G PG +G  G  GP G  G  GS G+KG          +G  GE G RG  G  G  G  G  GE+GD GP    G KG RGE G+KG +G +  G M   G  G+ GE G P  +      +   G +G  G +G  G VG +G PG  G  G  G KG+ GL G  G  GR G  GPPG TG  G  G+PG+ G  G+ G  G  G  G  G  G +G  G  GG                    GPQG+ G  G+ GP G  G+ G  GLP      GP G  G  G  G  GP G  G  G  GP G  G  G AGL     G G QG  G+ G  G PG+ G+ G+RG  G  G             +G  G  G  G  G +G  G+ G PGQKG+  +KG  G   +    G KG+         PG  G  GP G+RG PG +G  G+ G  GP GE GE
Sbjct:   91 RGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLHGQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPY---GAKGPRGEHGLKGEKGASCYGPM-KPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGATGKPGLLGPPGPPGGGRGTPGPPGPKGPRGYVGAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGDAGLP----GYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGA-------PGDSKLGRPGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGE 625          

HSP 9 Score: 67.781 bits (164), Expect = 1.138e-10
Identity = 205/578 (35.47%), Postives = 244/578 (42.21%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---------------------QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL----------------GKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGV--------------KGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV---GSKGEPGPPGLTGPQGKRGNPGPKGETGL-----MGPIGPVGEDGE 4042
            RGPPG RG    PG+ G DG  G  GP G+ G  G++G PG                      +G  GA G  G  G  G++G  G  GP+G  G KGE+G  G+ G  G  G  G  G  G PG                  E G  G  G KG  G  G  GEKG+ G                  G+TG+ G  GE G  G  G TGL G+ GP    G PG  G  G+N                              G +G KG+ G +G  G PG+ G  G PG+ G  G  G+PG+              +G KGD G  GP G  G D               G  G  G  G++G  GV G  GI G  G  G +GP G+ GP G  G  G  G  G  G  G  G  G  G+ G  G  G  GP G +G  G  G+RG  G PG++G  G +G  G  G  GP G RG PG  G MG  G+ G  G+ G  G  G PG+KG +   G+ G PG  G     G KGEPGP GL G  G  G PG  GE GL      G  GP GE G+
Sbjct:  596 RGPPGERG---YPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKG-------------EPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGP---QGPPGVEGPRGLN------------------------------GPRGEKGNQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGAD---------------GSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATGLTGNDGPVGGRGPPGAPGLMGIKGDQGLAGAPGQQGLDGMPGEKGNQGFPGLDGPPGLPGDASEKGQKGEPGPSGLRGDTGPAGTPGWPGEKGLPGLAVHGRAGPPGEKGD 1109          

HSP 10 Score: 59.3066 bits (142), Expect = 4.244e-8
Identity = 103/287 (35.89%), Postives = 128/287 (44.60%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP---------QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGP 3322
            G RG  GL G+PG+ G  G IGP G  G RG +G+PG     G  GA G  G TG  G KG+RG  G +G  GDKG+RG  G  G PG  G                              +KG  G  G  G+ GP+G  GE+G  GP G+ G  GT    G+ G +G+ G++   GP          G  GEPG PG+ G+                            G +GE+G KG+ GLIG TG  GR G  G  G+ G  G EG  G+ G KG+ G   P
Sbjct: 1321 GERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVG------------------------------QKGDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRDGT---PGLPGQKGEPGMLPPPGPKGEPGQPGRNGPKGEPGRPGERGL---------------------------IGIQGERGEKGERGLIGETGNVGRPGPKGDRGEPGERGYEGAIGLIGQKGEPGAPAP 1547          

HSP 11 Score: 56.9954 bits (136), Expect = 2.085e-7
Identity = 202/546 (37.00%), Postives = 247/546 (45.24%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRG--------LLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEG---KTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEA---------GPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGE---AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP--------GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G  GP GL G  G+ G +G  G KG  G  G+ GN GP G +G +G +G  G  GS G  G  GP GP G KG+                  +   G  G KG  G+ G PG +G  GE+G +G         LGR G+ G  G  G+KGD    G+ G  G +G++GIKG  G    +   G +G +G SG PG PG  G        G  G +G  G  G  GI GQ GP GEKG  G  GL G TG PG+             G  G  GA GA+G  G KG +G  GI GP+GE G  G  G  G+ G  G  G+ GR G  G  G Q ++GE G  G  G KG+ G  G  G  G  G    D    P  G+            G PG+ G+ GP G  G+ GE    G +G+ GP G  G  G RG NGP G                            +G  G VG PG  G+  L+GI   PG  G  G +GEP        GPPGL G QG++G+ GP G  G  G  G VG  G+
Sbjct:  413 GAPGPQGLNGVDGLPGPQGYNGQKGGAGLPGRPGNEGPPGKKGEKGTAGLNGPKGSIGPIGHPGPPGPEGQKGD----------------AGLPGYGIQGSKGDAGIPGYPGLKGSKGERGFKGNAGAPGDSKLGRPGTPGAAGAPGQKGDA---GRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGAR------GPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPG-QSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKGNK-----------GEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRGLNGPRG---------------------------EKGNQGAVGVPGNPGKDGLRGI---PGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGD 891          

HSP 12 Score: 55.8398 bits (133), Expect = 4.374e-7
Identity = 50/107 (46.73%), Postives = 61/107 (57.01%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPS--GQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPG--IKGSRGPVGNQGRDGEK 2137
            KG RGIRG  G      P   G  G++GE G  G KGE G +G  GP G+AG KGD G+QG  G  G +G  G +G +GP G  G PG  IKG +G  G  GR+G +
Sbjct: 1206 KGERGIRGQPGLPATV-PDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQ 1311          

HSP 13 Score: 55.0694 bits (131), Expect = 8.726e-7
Identity = 119/377 (31.56%), Postives = 149/377 (39.52%), Query Frame = 2
Query: 2900 IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGS-------GTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGE---PGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGET 4000
            I   G +G  GPLG TGLKG  G  G++G  G  G  G  GP G  G+ G  G  G++                                 G  G  G+ GP G PG       PG  G  G +GQ G+ G +G +G+ GPRG  G  G  G  G    + G    G +G  G  G  G+ G  G  G  G +G+ GP G  GP G  G  G+ GA                  PG PG  G +G    S          G  G+ G +G PG+ G  G+ GP G                                GLDG+ GE   PG  G+R  +G  G PG+ G  G +GEPG  GL G  G++G PG  G T
Sbjct:   85 IAEKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGERGSPGLH---------------------------------GQAGVPGVQGPAGNPGA------PGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGPQGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPM-----------KPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEG------------------------------DTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKGDRGEPGLNGLPGNPGQKGEPGRAGAT 381          

HSP 14 Score: 54.6842 bits (130), Expect = 1.007e-6
Identity = 114/287 (39.72%), Postives = 132/287 (45.99%), Query Frame = 2
Query: 3167 EKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG--IVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSP---GTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            EKG +G  G +GPTG  G +G  G  G +G  G +G PG  G +GD G    RG  GL             G  G PG QG  GN G  G+ G+ G  G  G  G  G  G+PGP G +G  G  G  G P         G  G PG  G AGL GP    G  GE GERGD GP G  G  G+ G  G  G    GP  P           P  +   K +  + G  G+ GQKGE  L G KG P   G  G  G KGE G PG  G +G++GN GP G TG  G
Sbjct:   87 EKGNRGLPGPLGPTGLKGEMGFPGMEGPSGDKGQKGDPGPYGQRGDKGE---RGSPGLH------------GQAGVPGVQGPAGNPGAPGINGKDGCDGQDGIPGLEGLSGMPGPRGYAGQLGSKGEKGEPAKENGDYAKGEKGEPGWRGTAGLAGP---QGFPGEKGERGDSGPYGAKGPRGEHGLKGEKGASCYGPMKPGAPGIKGEKGEPASSFPVKPTHTVMGPRGDMGQKGEPGLVGRKGEPGPEGDTGLDGQKGEKGLPGGPGDRGRQGNFGPPGSTGQKG 355          

HSP 15 Score: 54.6842 bits (130), Expect = 1.105e-6
Identity = 51/115 (44.35%), Postives = 60/115 (52.17%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVG------------TRGEVGMKGVKGEYGIQGPRG---PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G  G+ G  G KGD GP+G +G             RG+ G+ GV G  GI G +G   P G AGV G  G+ G  G RG+DG KG+ GS   PG  G PG KG RG  GN G  G
Sbjct:  857 GPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGS---PGLVGMPGNKGDRGAPGNDGPKG 968          

HSP 16 Score: 54.299 bits (129), Expect = 1.398e-6
Identity = 190/519 (36.61%), Postives = 228/519 (43.93%), Query Frame = 2
Query: 2495 PGTRGPSGLPG------KPGIDGKEGSIGPKGDLG---------PRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGK---FGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG--------IKGS---EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEG---ELGIQGPPGRKGELGP-----IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPG 3940
            PGT G +G PG      +PG  G++G +G KGD+G         P+G  G  G  GI G  G  GP GE G  G+RG     G  GP G+KGE G  G  G  G  G      ++     KG KG  G PG +G  G KG  GL G  G  G  G  GEKG  G  G  G  G  G  G        IKG     G+ G  G +G  G SGE G PG   ++  K                             KG  G  G+ GP G  G  GE G  G  G +G +G PGV+G +G   + GPRGE+G                           N G  GV G   + G++G PGR G+ GP     I  PGP+G  G +G  G  G  G +G  G  G  GS G PG  G  G  G SG  G  GE+GD GP G AG  G  G  G  G+ G DG +G+PG  G +G  GN G +G+ G DG  G  G  G    +G  G PG  GA G KG  G
Sbjct:  533 PGTPGAAGAPGQKGDAGRPGTPGQKGDMGIKGDVGGKCSSCRAGPKGDKGTSGLPGIPGKDGARGPPGERGYPGERGHDGINGQTGPPGEKGEDGRTGLPGATGEPGKPALCDLSLIEPLKGDKGYPGAPGAKGVQGFKGAEGLPGIPGPKGEFGFKGEKGLSGAPGNDGTPGRAGRDGYPGIPGQSIKGEPGFHGRDGAKGDKGSFGRSGEKGEPGSCALDEIKMPAKG-----------------------NKGEPGQTGMPGPPGEDGSPGERGYTGLKGNTGPQGPPGVEGPRG---LNGPRGEKG---------------------------NQGAVGVPGNPGKDGLRGIPGRNGQPGPRGEPGISRPGPMGPPGLNGLQGEKGDRGPTGPIGFPGADGSVGYPGDRGDAGLPGVSGRPGIVGEKGDVGPIGPAGVAGPPGVPGIDGVRGRDGAKGEPGSPGLVGMPGNKGDRGAPGNDGPKGFAGVTGAPGKRGPAGIPGVSGAKGDKGATG 998          

HSP 17 Score: 53.5286 bits (127), Expect = 2.112e-6
Identity = 103/295 (34.92%), Postives = 126/295 (42.71%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKP-------GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPD---GEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLD 3343
            GP G RG  G PG P       G  G +G  G  G+ G +G+ G  GP G+ GA+G  G  G  G+ G  G  G +G  G +GE                                           GEKG+ G  GR G  G IG     GE+G  G  G+ GL G  G +G  GS+G+ GL GS G  G  G PG+PG  G                 GP G +G  G  G  G+KG KGD GL GP+G PG +G+    G TG  G  G  G  G  G+ G  GP+G  G D
Sbjct: 1204 GPKGERGIRGQPGLPATVPDIRGDKGSQGERGYTGEKGEQGERGLTGPAGVAGAKGDRGLQGPPGASGLNGIPGAKGDIGPRGE----------------------------------IGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDT---------------GPQGFKGERGLNGFEGQKGDKGDRGLQGPSGLPGLVGQK---GDTGYPGLNGNDGPVGAPGERGFTGPKGRDGRD 1446          

HSP 18 Score: 53.5286 bits (127), Expect = 2.607e-6
Identity = 48/116 (41.38%), Postives = 62/116 (53.45%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVG-----TRGEVGM---------KGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA G+ G  GAKGD GP G++G      +GE G+         +G+ G  G+ G RG  G+AG  G +G+ G  GP G  G +G  GS G PG  G PG  G +G  G QG  GE+
Sbjct: 1268 GASGLNGIPGAKGDIGPRGEIGYPGVTIKGEKGLPGRPGRNGRQGLIGAPGLIGERGLPGLAGEPGLVGLPGPIGPAGSKGERGLAGSPGQPGQDGFPGAPGLKGDTGPQGFKGER 1383          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Fly
Match: vkg (gene:FBgn0016075 transcript:FBtr0333541)

HSP 1 Score: 84.7297 bits (208), Expect = 8.747e-16
Identity = 191/546 (34.98%), Postives = 242/546 (44.32%), Query Frame = 2
Query: 2519 LPGKPGIDGKEGSIGPKGDLGPRGQSGNP------------------GPQGIRGARGQSGPTGELGSKGDRGKFGP-----------QGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGV-----NXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPI-----GIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVG 4030
            L G+PG DG +G  G  GD G +G  G P                  GP+G++GA+G+ G  G  G  GDRG  G             GPTGD G++G PG  G  G                                 E G+ G +G KG  G  G +  +G++GP G++G+KG  G+VG   + G+ GL G  GP+GV GE G  G  G      N  +      G  GP G  G  G     G +G+KG  G +G  G  GA G IG +G  G  G +G +G PG  G  G  G++G  GE+G                  + G  G  G+ GP+G  GE G   P G  G+ GP G PG  G  G  G+ G                  +PG PG+ G +G A  SG +G+ GE G  GP   PG  G  GQRGP G       G+ G  G  G PGP G  G  G  G +G RGL G+ G PG+ G + L G  G  G  G  G+ G PGP G  GPQG+ G  G  G  G +G  GP G
Sbjct:  684 LIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGLPGRDG---------------------------------EPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVG---APGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERG------------------EIGYNGRQGDIGPRGPNGEFG---PKGLSGDDGPDGYPGANGLPGRKGETG------------------NPGFPGRPGAKGVAAYSGIKGDDGESGLTGPIGYPGAPGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRG 1154          

HSP 2 Score: 71.633 bits (174), Expect = 8.352e-12
Identity = 197/523 (37.67%), Postives = 238/523 (45.51%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL-----GKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGP------PGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            R   GLPG  G +G  G  G KG  G      N GP   RG RGQ G TG  GS G+RG  G  GP G +G +G+PG  G  G                                   G  G+ G  G VG  GPDG+  +VG L     G TG  G RG+ G  G +G+ G  GS+G +G +G+ G  GDAG  G  GE G+ G  G  G           G  G  G+KG+ G  G TG  G  GE  P       G+ G  G  G  GVKG KG+ G +G RGE G               G   PG  G+ G  GP G  G+ G  G PGR GE G  G  G  G  G  G++   G  G  G++G  G  G  G PG+ GL G  G  G +GE G  G  G  G+ GN GQRG +  +G  GP G  G+ G   P G  G  G  GS G +G+ G  G  G    +G+ G+ G  G  GS G PG PG+ G  G+RG  G  G  G +GP GP GE G
Sbjct:  503 R---GLPGDDGYNGLRGLPGEKGLRGDDCPVCNAGP---RGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQGNPGRAGHKGL---------------------------------PGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPGRNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIG----------DRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRG-DFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFG 975          

HSP 3 Score: 70.4774 bits (171), Expect = 1.584e-11
Identity = 214/567 (37.74%), Postives = 261/567 (46.03%), Query Frame = 2
Query: 2486 RGPPG---TRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELG---SKGDRGKF--------------GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTG---APGRIGEAGPPGKT--------GASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPG----LPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXX-----------XXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP--GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG    +G  G PG+ GI G  G +G  G  G    +G PGP+G++G +G  G  G+ G   +KG RG F              G Q P G KG++G+ GS G  G  G  G IG +G  G  G  G+ G PG  G  G  G+ G +G +G +G  G  G+ G  GP G+ G KG  G+ G  G  G  GL G +G  G  G PG PG  GV    G                            KG  G+ GL GP G   APG  G+ GP G +        G  G  G PG  G  G  G+ G RG++GL G  G  G  G KG  G PGR G    +G +G  G  G QGP G +GE G +GL G  G  G  G  G  G  G  G+ GD+G VG PG    L  ++  +G  G  G QGE GE+GD GP G  G  G +G  G IG  G  G  G  G  G  G                G +G  GLDG+ G PG  G++   G  G  G  GA+G +G    GPPG   PQG  G PG  G  G  G IGP GE G+
Sbjct:  804 RGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYG---LAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFGPKGLSGDDGPDGYPGANGLPGRKGETGNPGFPGRPGAKGVAAYSGI---------------------------KGDDGESGLTGPIGYPGAPGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGI---NGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDMGPIGFIGPPGAKGERGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGHRGPQIQGPPG---PQGDVGFPGAPGHNGRHGLIGPKGELGD 1334          

HSP 4 Score: 64.6994 bits (156), Expect = 8.719e-10
Identity = 191/551 (34.66%), Postives = 229/551 (41.56%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGP--------RGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSR--GPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR---IGEAGPPGKTGAS------------GAEGQPGVKGTKGDTGILGPRGEQGL--DXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ------GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV---GSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            +G PG  G  GLPG  GI G+ G +G  G  G         +G+ G+ G  G RG  G  G  G  GS G +G+ G  G  GD G+ G  G  G PG  G +G  G   +       G  G  G +G  G+ G  G  G KG   P       G  GE G  GP G  G KG +G +G++G  G  G  G    GP G  G PG  GD G                       G PG PG  GE G+ G IG  G  G PG+    GE GPPG++G              G  G+PG KG KG+ G  G  G+ GL  +         G  G +G+PGR G     G +G +GE+G  G  G+ G  G IG  G                                GL G AGLQG  G  G        GE GERG+ G  G  G++G RGPNG  G                  P G  G  G  G  G  G PG+KGE    G  G PG  G     G KG+ G  GLTGP G  G PG KG+ G +G
Sbjct:  569 QGNPGRAGHKGLPGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPGRNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQ---------------------GAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSG------------------------------RRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFG------------------PKGLSGDDGPDGYPGANGLPGRKGETGNPGFPGRPGAKGVAAYSGIKGDDGESGLTGPIGYPGAPGAKGQRGPVG 1050          

HSP 5 Score: 62.003 bits (149), Expect = 6.976e-9
Identity = 183/536 (34.14%), Postives = 223/536 (41.60%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEG------SIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKF------GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXX-XXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSG--EPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP--TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGET---GLMGPIGPVGE 4033
            +G  G+ GP+GLPG+ G+ G+ G        G  G+ G +G  G PG  GI G +G +G  G  G KG +G+       G  G  GD+G RG  G QG  G  G  G +G+       +      G  G QG  GE+G +G +G  G +GP G  GE+GD+G           G++G  G+EG  G  G +GP+G  G   P   GD GV          G  G  G  G  G  G PGP GE G  G IG  GP   G PG  G+ G PG  G +G  G  G KG  GD G  G RGE G                             G QG  G++G QG PG  G  G  G                                  PGLPGK G  G  G  G  G+AG  G  G  G+ G  GQ G      +                     PG +G  GLDG  GE G  G   L G +G  G +G  G +GE G  G  GPQG+RG+ G  G T   GL G  GP GE
Sbjct: 1063 KGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDMGPIGFIGPPGAKGERGDIG---------YAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGV---------AGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGHRGPQIQGPPGPQGDVGFPGAPGHNGRHGLIGPKGELGDMGRQGERGESGY-------------------------AIVGRQGDIGDIGFQGEPGWDGAKGEQGY---------------------------------PGLPGKNGRVGAPGPRGPTGDAGWGGIDGMDGLVGPKGQPGVTYSYSMA-------------------RPGDRGEPGLDGFQGEEGDGGAPGLIGFQGQRGAVGYRGDQGEVGYTGADGPQGQRGDKGYMGLTGAPGLRGLPGPQGE 1503          

HSP 6 Score: 60.8474 bits (146), Expect = 1.368e-8
Identity = 137/418 (32.78%), Postives = 170/418 (40.67%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGV---------SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPG-----VKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELG-----PIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG------ETGLMGPIGPVGE 4033
            KG++G +G  G +G  G +G  GD+GP G+ G  G +G+VG  G +G+ G  G  GP+G          SGEPG+PG  G++                                 G  G  G+ GP+GAPG+ G  GPPGK G  G  G+ G      KGTKG+      RGE          G  G  G  G  G +GS G++G  G+ GE G  G PG KG+ G     P  L GP G  G  G   +                   G  G  GLQG  G  G  GE G  G  G PG  G +G  G                                  RG  GK GEPG+ G++ +KG  G  G  G  GS GE G  G TG  G +G  GP G         L GPIG  G+
Sbjct:   41 KGIKGRMGAPGPIGVPGLEGPAGDIGPPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGPRGID---------------------------------GCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGN------RGET---------GQPGGVGPPGFDGDRGSKGDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLIGPPGAKGEPGDSLSGVLKPDDTLK---------GYKGYVGLQGDEGPQGPTGEQGAVGRNGLPGARGEIGGPG---------------------------------ERGKPGKDGEPGRFGDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTKSLPGPIGSQGD 368          

HSP 7 Score: 53.9138 bits (128), Expect = 1.632e-6
Identity = 176/549 (32.06%), Postives = 214/549 (38.98%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKP---------GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRG---------------ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP--IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTG----LMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G PG  G  GL G P         G+ G +G  GP G   P      PGP G +G                 G+ GP G  G  GD G  G +GP G + ER                        G+ G  G +G PGPQGP GE G+ G  G  G  G   +GP GE G  G  G  G +G RGEVG+ G +G  G    ++G  G QG  G  G PGD G N                        G  G  GEKG++GD         P  +  AGP G  G  G  G PG  G +G  G+ GPRG QGL                      G+ G +G +G+ G  GI G PG+ G  GP G    VG+                            G  G+ G  G  G  G +GE G+RGD G  G  G  G+ G +G  G              G           G  G DG  GE G  G+   KG+KG P       + GEPG  G TGP+G +G  G +G  GL G IG  G  GE
Sbjct:  312 KGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTKSLPGPIGSQGDIGPPGEQGPPGLPGKPGRRGPIGLAGQSGDPGLNGSRGPPG-RSER------------------------GEAGDYGFIGPPGPQGPPGEAGLPGRYGLHGEPGQNVVGPKGEPGLNGQPGLEGYRGDRGEVGLPGDKGLPGEGYNIVGPPGSQGPPGFRGLPGDDGYN------------------------GLRGLPGEKGLRGDD-------CP--VCNAGPRGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQ---------------------GNPGRAGHKGLPGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDT----------GDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGA------------PGRNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGE 759          

HSP 8 Score: 53.1434 bits (126), Expect = 3.452e-6
Identity = 112/308 (36.36%), Postives = 132/308 (42.86%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERX-----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVG-----PLGKTGLKGTRGEVG------------IKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            GPPG  GP G  G  G  G++G  G +GD+GP+G+ G PG  G  G  G  GP G  G  G  G  GP G  G  G RG                G     G  G+ G+ G  G VG PG  G  G KG  G  G  G  G  G  G KGD G     P    G  G +GE G            +KG +G  GL G  GPQG +GE G+ G  G+                           PG RGE G  G+ G  G  G PGR G+    G PG TGA G +G PG +G  G TG+ G +G  G
Sbjct:   66 GPPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGPRGIDGCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGNRGETGQPGGVGPPGFDGDRGSKGDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLIGPPGAKGEPGDSLSGVLKPDDTLKGYKGYVGLQGDEGPQGPTGEQGAVGRNGL---------------------------PGARGEIGGPGERGKPGKDGEPGRFGDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAG 346          

HSP 9 Score: 52.7582 bits (125), Expect = 3.661e-6
Identity = 46/96 (47.92%), Postives = 53/96 (55.21%), Query Frame = 2
Query: 1844 IRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            I G  GAKG  GP+G  G +G  G+ G  GE G+ G  G +G  GV G   IQGE GP G+ G KG  G VG PG  G  G  G +G  G QG DG
Sbjct:  761 IPGPVGAKGYPGPTGDYGQQGAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDG 856          

HSP 10 Score: 51.6026 bits (122), Expect = 8.008e-6
Identity = 43/91 (47.25%), Postives = 51/91 (56.04%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIA--GVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGP 2107
            G  G  GAKG+ G  G  G  G  G +G+KG  G QGPRGP GI     KGD G+ G  G  G+ G+ G +G+ GPPG  G  G  G RGP
Sbjct: 1209 GFIGPPGAKGERGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGHRGP 1299          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Fly
Match: vkg (gene:FBgn0016075 transcript:FBtr0079036)

HSP 1 Score: 84.7297 bits (208), Expect = 8.747e-16
Identity = 191/546 (34.98%), Postives = 242/546 (44.32%), Query Frame = 2
Query: 2519 LPGKPGIDGKEGSIGPKGDLGPRGQSGNP------------------GPQGIRGARGQSGPTGELGSKGDRGKFGP-----------QGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGV-----NXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPI-----GIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVG 4030
            L G+PG DG +G  G  GD G +G  G P                  GP+G++GA+G+ G  G  G  GDRG  G             GPTGD G++G PG  G  G                                 E G+ G +G KG  G  G +  +G++GP G++G+KG  G+VG   + G+ GL G  GP+GV GE G  G  G      N  +      G  GP G  G  G     G +G+KG  G +G  G  GA G IG +G  G  G +G +G PG  G  G  G++G  GE+G                  + G  G  G+ GP+G  GE G   P G  G+ GP G PG  G  G  G+ G                  +PG PG+ G +G A  SG +G+ GE G  GP   PG  G  GQRGP G       G+ G  G  G PGP G  G  G  G +G RGL G+ G PG+ G + L G  G  G  G  G+ G PGP G  GPQG+ G  G  G  G +G  GP G
Sbjct:  684 LIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGLPGRDG---------------------------------EPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVG---APGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERG------------------EIGYNGRQGDIGPRGPNGEFG---PKGLSGDDGPDGYPGANGLPGRKGETG------------------NPGFPGRPGAKGVAAYSGIKGDDGESGLTGPIGYPGAPGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRG 1154          

HSP 2 Score: 71.633 bits (174), Expect = 8.352e-12
Identity = 197/523 (37.67%), Postives = 238/523 (45.51%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL-----GKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGP------PGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            R   GLPG  G +G  G  G KG  G      N GP   RG RGQ G TG  GS G+RG  G  GP G +G +G+PG  G  G                                   G  G+ G  G VG  GPDG+  +VG L     G TG  G RG+ G  G +G+ G  GS+G +G +G+ G  GDAG  G  GE G+ G  G  G           G  G  G+KG+ G  G TG  G  GE  P       G+ G  G  G  GVKG KG+ G +G RGE G               G   PG  G+ G  GP G  G+ G  G PGR GE G  G  G  G  G  G++   G  G  G++G  G  G  G PG+ GL G  G  G +GE G  G  G  G+ GN GQRG +  +G  GP G  G+ G   P G  G  G  GS G +G+ G  G  G    +G+ G+ G  G  GS G PG PG+ G  G+RG  G  G  G +GP GP GE G
Sbjct:  503 R---GLPGDDGYNGLRGLPGEKGLRGDDCPVCNAGP---RGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQGNPGRAGHKGL---------------------------------PGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPGRNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIG----------DRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRG-DFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFG 975          

HSP 3 Score: 70.4774 bits (171), Expect = 1.584e-11
Identity = 214/567 (37.74%), Postives = 261/567 (46.03%), Query Frame = 2
Query: 2486 RGPPG---TRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELG---SKGDRGKF--------------GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTG---APGRIGEAGPPGKT--------GASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPG----LPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXX-----------XXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP--GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG    +G  G PG+ GI G  G +G  G  G    +G PGP+G++G +G  G  G+ G   +KG RG F              G Q P G KG++G+ GS G  G  G  G IG +G  G  G  G+ G PG  G  G  G+ G +G +G +G  G  G+ G  GP G+ G KG  G+ G  G  G  GL G +G  G  G PG PG  GV    G                            KG  G+ GL GP G   APG  G+ GP G +        G  G  G PG  G  G  G+ G RG++GL G  G  G  G KG  G PGR G    +G +G  G  G QGP G +GE G +GL G  G  G  G  G  G  G  G+ GD+G VG PG    L  ++  +G  G  G QGE GE+GD GP G  G  G +G  G IG  G  G  G  G  G  G                G +G  GLDG+ G PG  G++   G  G  G  GA+G +G    GPPG   PQG  G PG  G  G  G IGP GE G+
Sbjct:  804 RGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYG---LAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFGPKGLSGDDGPDGYPGANGLPGRKGETGNPGFPGRPGAKGVAAYSGI---------------------------KGDDGESGLTGPIGYPGAPGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGI---NGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDMGPIGFIGPPGAKGERGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGHRGPQIQGPPG---PQGDVGFPGAPGHNGRHGLIGPKGELGD 1334          

HSP 4 Score: 64.6994 bits (156), Expect = 8.719e-10
Identity = 191/551 (34.66%), Postives = 229/551 (41.56%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGP--------RGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSR--GPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR---IGEAGPPGKTGAS------------GAEGQPGVKGTKGDTGILGPRGEQGL--DXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ------GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV---GSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            +G PG  G  GLPG  GI G+ G +G  G  G         +G+ G+ G  G RG  G  G  G  GS G +G+ G  G  GD G+ G  G  G PG  G +G  G   +       G  G  G +G  G+ G  G  G KG   P       G  GE G  GP G  G KG +G +G++G  G  G  G    GP G  G PG  GD G                       G PG PG  GE G+ G IG  G  G PG+    GE GPPG++G              G  G+PG KG KG+ G  G  G+ GL  +         G  G +G+PGR G     G +G +GE+G  G  G+ G  G IG  G                                GL G AGLQG  G  G        GE GERG+ G  G  G++G RGPNG  G                  P G  G  G  G  G  G PG+KGE    G  G PG  G     G KG+ G  GLTGP G  G PG KG+ G +G
Sbjct:  569 QGNPGRAGHKGLPGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGAPGRNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQ---------------------GAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSG------------------------------RRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFG------------------PKGLSGDDGPDGYPGANGLPGRKGETGNPGFPGRPGAKGVAAYSGIKGDDGESGLTGPIGYPGAPGAKGQRGPVG 1050          

HSP 5 Score: 62.003 bits (149), Expect = 6.976e-9
Identity = 183/536 (34.14%), Postives = 223/536 (41.60%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEG------SIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKF------GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXX-XXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSG--EPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP--TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGET---GLMGPIGPVGE 4033
            +G  G+ GP+GLPG+ G+ G+ G        G  G+ G +G  G PG  GI G +G +G  G  G KG +G+       G  G  GD+G RG  G QG  G  G  G +G+       +      G  G QG  GE+G +G +G  G +GP G  GE+GD+G           G++G  G+EG  G  G +GP+G  G   P   GD GV          G  G  G  G  G  G PGP GE G  G IG  GP   G PG  G+ G PG  G +G  G  G KG  GD G  G RGE G                             G QG  G++G QG PG  G  G  G                                  PGLPGK G  G  G  G  G+AG  G  G  G+ G  GQ G      +                     PG +G  GLDG  GE G  G   L G +G  G +G  G +GE G  G  GPQG+RG+ G  G T   GL G  GP GE
Sbjct: 1063 KGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKGLGGYPGRNGINGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDMGPIGFIGPPGAKGERGDIG---------YAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGV---------AGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGHRGPQIQGPPGPQGDVGFPGAPGHNGRHGLIGPKGELGDMGRQGERGESGY-------------------------AIVGRQGDIGDIGFQGEPGWDGAKGEQGY---------------------------------PGLPGKNGRVGAPGPRGPTGDAGWGGIDGMDGLVGPKGQPGVTYSYSMA-------------------RPGDRGEPGLDGFQGEEGDGGAPGLIGFQGQRGAVGYRGDQGEVGYTGADGPQGQRGDKGYMGLTGAPGLRGLPGPQGE 1503          

HSP 6 Score: 60.8474 bits (146), Expect = 1.368e-8
Identity = 137/418 (32.78%), Postives = 170/418 (40.67%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGV---------SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPG-----VKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELG-----PIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG------ETGLMGPIGPVGE 4033
            KG++G +G  G +G  G +G  GD+GP G+ G  G +G+VG  G +G+ G  G  GP+G          SGEPG+PG  G++                                 G  G  G+ GP+GAPG+ G  GPPGK G  G  G+ G      KGTKG+      RGE          G  G  G  G  G +GS G++G  G+ GE G  G PG KG+ G     P  L GP G  G  G   +                   G  G  GLQG  G  G  GE G  G  G PG  G +G  G                                  RG  GK GEPG+ G++ +KG  G  G  G  GS GE G  G TG  G +G  GP G         L GPIG  G+
Sbjct:   41 KGIKGRMGAPGPIGVPGLEGPAGDIGPPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGPRGID---------------------------------GCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGN------RGET---------GQPGGVGPPGFDGDRGSKGDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLIGPPGAKGEPGDSLSGVLKPDDTLK---------GYKGYVGLQGDEGPQGPTGEQGAVGRNGLPGARGEIGGPG---------------------------------ERGKPGKDGEPGRFGDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTKSLPGPIGSQGD 368          

HSP 7 Score: 53.9138 bits (128), Expect = 1.632e-6
Identity = 176/549 (32.06%), Postives = 214/549 (38.98%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKP---------GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRG---------------ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP--IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTG----LMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G PG  G  GL G P         G+ G +G  GP G   P      PGP G +G                 G+ GP G  G  GD G  G +GP G + ER                        G+ G  G +G PGPQGP GE G+ G  G  G  G   +GP GE G  G  G  G +G RGEVG+ G +G  G    ++G  G QG  G  G PGD G N                        G  G  GEKG++GD         P  +  AGP G  G  G  G PG  G +G  G+ GPRG QGL                      G+ G +G +G+ G  GI G PG+ G  GP G    VG+                            G  G+ G  G  G  G +GE G+RGD G  G  G  G+ G +G  G              G           G  G DG  GE G  G+   KG+KG P       + GEPG  G TGP+G +G  G +G  GL G IG  G  GE
Sbjct:  312 KGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTKSLPGPIGSQGDIGPPGEQGPPGLPGKPGRRGPIGLAGQSGDPGLNGSRGPPG-RSER------------------------GEAGDYGFIGPPGPQGPPGEAGLPGRYGLHGEPGQNVVGPKGEPGLNGQPGLEGYRGDRGEVGLPGDKGLPGEGYNIVGPPGSQGPPGFRGLPGDDGYN------------------------GLRGLPGEKGLRGDD-------CP--VCNAGPRGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQ---------------------GNPGRAGHKGLPGPAGIPGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDSGHRGDT----------GDDGEKGRDGSDGSKGERGETGQRGDYGDAGYQGRDGEPGRDGRDGA------------PGRNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNTGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGE 759          

HSP 8 Score: 53.1434 bits (126), Expect = 3.452e-6
Identity = 112/308 (36.36%), Postives = 132/308 (42.86%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERX-----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVG-----PLGKTGLKGTRGEVG------------IKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            GPPG  GP G  G  G  G++G  G +GD+GP+G+ G PG  G  G  G  GP G  G  G  G  GP G  G  G RG                G     G  G+ G+ G  G VG PG  G  G KG  G  G  G  G  G  G KGD G     P    G  G +GE G            +KG +G  GL G  GPQG +GE G+ G  G+                           PG RGE G  G+ G  G  G PGR G+    G PG TGA G +G PG +G  G TG+ G +G  G
Sbjct:   66 GPPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGPRGIDGCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGNRGETGQPGGVGPPGFDGDRGSKGDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLIGPPGAKGEPGDSLSGVLKPDDTLKGYKGYVGLQGDEGPQGPTGEQGAVGRNGL---------------------------PGARGEIGGPGERGKPGKDGEPGRFGDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAG 346          

HSP 9 Score: 52.7582 bits (125), Expect = 3.661e-6
Identity = 46/96 (47.92%), Postives = 53/96 (55.21%), Query Frame = 2
Query: 1844 IRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            I G  GAKG  GP+G  G +G  G+ G  GE G+ G  G +G  GV G   IQGE GP G+ G KG  G VG PG  G  G  G +G  G QG DG
Sbjct:  761 IPGPVGAKGYPGPTGDYGQQGAPGLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDG 856          

HSP 10 Score: 51.6026 bits (122), Expect = 8.008e-6
Identity = 43/91 (47.25%), Postives = 51/91 (56.04%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIA--GVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGP 2107
            G  G  GAKG+ G  G  G  G  G +G+KG  G QGPRGP GI     KGD G+ G  G  G+ G+ G +G+ GPPG  G  G  G RGP
Sbjct: 1209 GFIGPPGAKGERGDIGYAGQLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGHRGP 1299          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Zebrafish
Match: col27a1b (collagen, type XXVII, alpha 1b [Source:NCBI gene;Acc:560145])

HSP 1 Score: 162.925 bits (411), Expect = 1.091e-39
Identity = 257/862 (29.81%), Postives = 348/862 (40.37%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGP------KGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGA---EGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXX------XXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSG---TQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPG---PPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            GPPG +G  G PG PG+DG+ G  GP      +GD+G  G+SG  G  G +G  G++G  G +G KG  GK G  G  G  GE+G  G QG                                    + G  G LG +G  G IGP G +G  G +G TG  G  GE G+ G EG  G  GS G  G  GE G  GD G                                G  G  GDIG +G  G  G  G+ G PG  G  G    EG PGV G  G  G  GP+G +G                           N GP+G       +G  G +G  GP+G+PGP G  G  G +G      A G  G  G  GD G  G  GLPGK G QG AG  G    QG  G +G+RG PG +G  G+RG NG                                G  GK G+ G KG+    G +G PG +G  G +G PG   P G+ GP+G +G  G +G  G +G IGP G  G  G  G  G  GPPGP G  G P    +   Q        + + +          + T     +D    +F  +  ++  I  I+ P G  +++ AR C D+     +  DG Y+IDPN G   D+IEV C F+   +TC+  V                     + +LE  +S             QMNF+   +S++ Q I   C    ++     SE++K   ++++    +    +  G       G+++  + KDE            DG  ++T     +F   T D ++LPI++   N  + K  S   L V  +CF
Sbjct:  651 GPPGAKGILGEPGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQG------------------------------------QLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDG-----------------------------KVEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKG---------------------------NKGPKGKNSP---RGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGIPGQSGPPGKRGLNG------------------------------GMGFPGKQGDQGFKGQPGDAGEQGFPGVLGIFGPQGPPGDFGPVGVQGPKGPQGLMGMQGAIGPVGIIGPSGNPGPQGDKGNKGEMGPPGPRGPPGPPVSIFNPFYQENEALGAALHVFDSRSAIRSEMYQDTDL-PLLDQGSEIFKTLHYLSILIHSIKNPLG-TQENPARMCRDLLECEHRLNDGTYWIDPNLGCSSDNIEVTCSFTNGGQTCLKPV--------------------TASKLEIGVSL-----------IQMNFIHLLSSEAVQIITVHCLNISVWA----SEDSKTPSSSMVYFKAWDGQIIEAG-------GFIEPDLLKDE--------CWITDGRWHQT-----QFIFRTQDPNLLPIVEI-YNLPSTKPGSHYHLEVGPVCF 1329          

HSP 2 Score: 108.227 bits (269), Expect = 6.792e-23
Identity = 187/545 (34.31%), Postives = 242/545 (44.40%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIG---PKGDLGPRGQSGNPGPQGIRGARGQSGP--------TGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTG---LMGSRGPQGV---SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELG----IQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPN---GPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKR------GNPGPKGETGLMGPIGPVGEDG 4039
            P   G  G+ G PG+ G+ G +G   P G++G  G+ G+PG  G RG  G +G          GE G  G  G  G QGPTG  G +G                                            G  G  G  G  GP GPDG KG+ G +G  G  G +G +G KG+EG+TG   L+G +GP+G    +GE G PG+ G                                  KG +G +G +G  G  G  G  GP G  G +G  G  G  G  G+ G++G  G QG                      QGS G+ GP+G +GE G    ++GPPG  G++GP+G  G  G      + G PG PG  G  G+ G  G+PG+PG +G +G  G  G++G  G +G   P G +GN G  GP    GP G++G +G  G PGP G  G  G  G  G  G DG VG PG+ G +   G  G PG  G+ G KGE G PG +GP GKR      G PG +G+ G  G  G  GE G
Sbjct:  570 PKVCGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGHPGAPGERGLSGPAGAPVSHCYGKIGETGEAGPTGFTGVQGPTGPPGAKGIL------------------------------------------GEPGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKG---------------------------------SKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQG---------------------PQGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGPVGNRGDRG------EPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGIPGQSGPPGKRGLNGGMGFPGKQGDQGFKGQPGDAGEQG 1012          

HSP 3 Score: 105.145 bits (261), Expect = 5.128e-22
Identity = 192/537 (35.75%), Postives = 236/537 (43.95%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKP-----GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRG------ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGA---EGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXX------XXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGG---SGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVG 4030
            G PG RG SG  G P     G  G+ G  GP G  G +G +G PG +GI G        G++GP+G  G+KG++G  G +G +G+KG  G  G++G  G  G  G  G  G  G  G +G  G  G +G  G+ G  G LG +G  G IGP G +G  G +G TG  G  GE G+ G EG  G  GS G  G  GE G  GD G                                G  G  GDIG +G  G  G  G+ G PG  G  G    EG PGV G  G  G  GP+G +G                           N GP+G       +G  G +G  GP+G+PGP G  G  G +      GA G  G  G  GD G  G  GLPGK G QG AG     G QG  G +G+RG PG +G  G+RG NG                                G  GK G+ G KG+    G +G PG +G  G +   GPPG  GP G +G  GP+G  G+ G IGPVG
Sbjct:  610 GAPGERGLSGPAGAPVSHCYGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDG-----------------------------KVEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKG---------------------------NKGPKGKNSP---RGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGIPGQSGPPGKRGLNG------------------------------GMGFPGKQGDQGFKGQPGDAGEQGFPGVLGIFGPQ---GPPGDFGPVGVQGPKGPQGLMGMQGAIGPVG 1054          

HSP 4 Score: 80.4925 bits (197), Expect = 1.618e-14
Identity = 121/357 (33.89%), Postives = 149/357 (41.74%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGK-EGSIGPKGDLGPRGQSGN---------PGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP---IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVG 3517
            +G  G+ GP G  G+ G DGK EG  GP GD+GP G  G+         PG +G++G RG+ G  G  G+ G RG  GP+G  G+KG +                                    G   P GE G RG  G  G  GP   IG +G +GD GP G  G  G +G  G  G +G  GL G  GPQG +G PG PG  G                                G KG +G  G  GP G  G  G  G PGK G  G +GQPG  G +G  G+LG  G QG                          G+ GP GV+G  G QG  G +G +GP+G+ GP G
Sbjct:  794 QGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPK------------------------------------GKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQG------------------------------SFGPKGERGIPGQSGPPGKRGLNGGMGFPGKQGDQGFKGQPGDAGEQGFPGVLGIFGPQG------------------------PPGDFGPVGVQGPKGPQGLMGMQGAIGPVGIIGPSG 1060          

HSP 5 Score: 71.2478 bits (173), Expect = 1.097e-11
Identity = 138/409 (33.74%), Postives = 170/409 (41.56%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKF-GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGL---------IGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGD 3682
            +G  G  GP G  G  G  G  G +G  G+ G  G  G+ GPQG   + G  GP GE G +GD GK  GP GP+GD G                                           +G +G RG  G  G  G  G  GE+G  G  G  G  G RG  G KGS+G  G  G   P+G SG  GSPG  GV G +G  G++G +G  GP G  G  G  G  G+ G  GD+GL          G  G PG  G  GP G+ G  G  G PG +G  G  G  G +G+QG                       G  G++G QG  G LGI GP G  G+ GP+G+ GP G  G  G                       G  G  G+ GP+G  G QG+ G +G+
Sbjct:  752 QGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQG---SLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGP------------------------------------------VGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGIPGQSGPPGKRGLNGGMGFPGKQGDQGFK---------------------GQPGDAGEQGFPGVLGIFGPQGPPGDFGPVGVQGPKGPQGLMGMQ---------------------GAIGPVGIIGPSGNPGPQGDKGNKGE 1073          

HSP 6 Score: 64.6994 bits (156), Expect = 1.158e-9
Identity = 99/330 (30.00%), Postives = 130/330 (39.39%), Query Frame = 2
Query: 3167 EKGVKGDIGLIGPTGAPGRI----------------------GEAGPPGKTGASGAE--------GQPGVKGTKGDTGILGPRGEQGLDXXXXX--------XXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            E G +GD G+ G  G PG                        G+ GPPG+ G  G +        G PG+ G +G  G +GP GE GL G  G         +            G+ G TG +GP G  G  G  GPPG KG LG  G                                  PG+ G+AG  GP G  G QG+ G  G+ G  G+ G  G  G  G  G++G                         +G +GK+G+ G++G+   KG KG  G +G +G+ GE G  G  GP+G RG       TG MG  G +G+ GE
Sbjct:  515 EPGFQGDKGIQGSPGLPGVQGKPGPQVDFYVFVYYCFLKGFPGDIGPPGQNGVEGPKVCGEPGMTGNPGMLGERGPVGFVGPFGEMGLAGEKGHPGAPGERGLSGPAGAPVSHCYGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPG---------------------------------DPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGV------------------------KGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGT------TGFMGATGKMGQHGE 781          

HSP 7 Score: 63.5438 bits (153), Expect = 2.373e-9
Identity = 103/277 (37.18%), Postives = 126/277 (45.49%), Query Frame = 2
Query: 3242 PGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQG--------VEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGG--SGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            P + G  G  G+PG  G +GD GI G  G  G+     P               +G  G+ GP G        V GE G+ G PG  GE GP+G  GP G                  + G  G  G PG PG+ GL GPAG   S   G+ GE G+ GP G  G  G  GP G  GI+      G+PG  G  G  G  GP G++G  G VG  G+ GE+ + G KG+ G  G  G  G  GP G  G  G+RG PG KG  G  G +G +G  GE
Sbjct:  501 PCQQGELGLPGEPGEPGFQGDKGIQGSPGLPGVQ--GKPGPQVDFYVFVYYCFLKGFPGDIGPPGQNGVEGPKVCGEPGMTGNPGMLGERGPVGFVGPFG------------------EMGLAGEKGHPGAPGERGLSGPAGAPVSHCYGKIGETGEAGPTGFTGVQGPTGPPGAKGIL------GEPGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGE 751          

HSP 8 Score: 62.7734 bits (151), Expect = 3.970e-9
Identity = 46/102 (45.10%), Postives = 58/102 (56.86%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G  G +G +G  GP G  G  GE G  GV GE    GP GP+G  G +GD+G++GE+G +G  G KG EG  G PG  G  G +G  G  G +G+ GEK
Sbjct:  636 GEAGPTGFTGVQGPTGPPGAKGILGEPGDPGVDGE---AGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEK 734          

HSP 9 Score: 61.6178 bits (148), Expect = 9.078e-9
Identity = 47/117 (40.17%), Postives = 60/117 (51.28%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVG---------------PPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G RG+ G +GA   H   G++G  GE G  G  G  G  GP G +GI G  GD G+ GE GP G DG KG++G VG                 G  G+PG+ G +GP G  G+ GE+
Sbjct:  613 GERGLSGPAGAPVSHC-YGKIGETGEAGPTGFTGVQGPTGPPGAKGILGEPGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGER 728          

HSP 10 Score: 60.077 bits (144), Expect = 2.909e-8
Identity = 47/99 (47.47%), Postives = 55/99 (55.56%), Query Frame = 2
Query: 1844 IRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGN---PGIKGSRGPVGNQGRDG 2131
            + G  G  GD GP G  G RGE G  G  G  G+QG RG EG  GV G+ G +G  GP+G  GNKG +G   P G +GN   PG  G  GP G  GR+G
Sbjct:  815 VEGPPGPSGDIGPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREG 913          

HSP 11 Score: 57.3806 bits (137), Expect = 1.711e-7
Identity = 46/114 (40.35%), Postives = 57/114 (50.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPG-------------YAGNPGIKGSRGPVGNQGRDGE 2134
            G  G RG  G KG  G  GQ+G  G +G +G  G  G +G RG  G  G  G +G  GE G  G +G++G +GS+G PG               G PG  G  GPVGN+G  GE
Sbjct:  723 GKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDGKVEGPPGPSGDIGPVGNRGDRGE 836          

HSP 12 Score: 57.3806 bits (137), Expect = 1.893e-7
Identity = 47/105 (44.76%), Postives = 56/105 (53.33%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP---GYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G RG  G  GD G  G  G +GE G +G  G  G  GPRG  G  G KG+ G +G+  PRG+ GN+G  G VG P   G  G  G +G  GP G  G+DG K
Sbjct:  826 GPVGNRGDRGEPGDPGYPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAK 930          

HSP 13 Score: 56.6102 bits (135), Expect = 3.360e-7
Identity = 43/103 (41.75%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG    RG SG +G  GP G  G RG +G +G +G+ G  G  G +G  G+ GD+G  G+ G  GK G +G+ G+ G PG  G+ G KG RG  G  G  G++
Sbjct:  882 KGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGIPGQSGPPGKR 984          

HSP 14 Score: 53.9138 bits (128), Expect = 2.300e-6
Identity = 43/104 (41.35%), Postives = 51/104 (49.04%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +KG  G  G  G  G  GP G++G  GE G  G KG  G QG  G  G  G +GD G  G  G RG  G  G  G +G  G  G  G +G +GP G+ G  G K
Sbjct:  700 FKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPK 803          

HSP 15 Score: 53.1434 bits (126), Expect = 3.979e-6
Identity = 44/102 (43.14%), Postives = 53/102 (51.96%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            Y G  G++G  G +G  G  G  G RG  G KG KG  G +G   P G +G +G  G  G  GPRG  G +G EG  GP G AG  G KG  G +G+ G  G
Sbjct:  842 YPGLEGVQGERGKEGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVG 943          

HSP 16 Score: 52.7582 bits (125), Expect = 4.751e-6
Identity = 43/101 (42.57%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KG  G  G  G  G+ GPSG  G +GE G  G++GE G +G  G +G  G  GD G+ G  GP GK G  G+ G  G  G  G+ G  G  G +G QG  G
Sbjct:  656 KGILGEPGDPGVDGEAGPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTG 756          

HSP 17 Score: 52.7582 bits (125), Expect = 5.671e-6
Identity = 43/104 (41.35%), Postives = 58/104 (55.77%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP---GYAGNPGIKGSRGPVGNQGRDG 2131
            +GA G+ G+SG +G  GP G  G +G  G    +GE G +G  GP G+ G +G IG +G  G  G DG  G +G+ G P   G+ G+ G+ G  GP GN G  G
Sbjct:  855 EGAPGVPGNSGPRGFPGPKGSKGNKGPKGKNSPRGESGNRGSPGPVGVPGPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPG 958          

HSP 18 Score: 52.373 bits (124), Expect = 6.601e-6
Identity = 42/104 (40.38%), Postives = 56/104 (53.85%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYG---IQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G  G+ G +GD G  G+ G +G +G KG +G  G   + G +GPEG  G  G+ G  GE G +G  G  G+ G++G  G  G  G KGSRG  G  G  G+
Sbjct:  672 GPSGPDGAKGEQGDVGLEGESGEKGVIGFKGTEGRTGDPGLIGVKGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGK 775          

HSP 19 Score: 52.373 bits (124), Expect = 7.366e-6
Identity = 40/99 (40.40%), Postives = 53/99 (53.54%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGR 2125
            KG  G  G +G +G  G  G  G +G++G  G  GE G  G  GP+G  G  G +G  G+ G  G+ G  G EG  GP G  G+PG KG +G  G+ G+
Sbjct:  716 KGPEGKIGKTGERGKPGEKGSKGHQGQLGEMGALGEQGDTGFIGPKGSRGTTGFMGATGKMGQHGEPGLVGYEGHQGPQGSLGSPGPKGEKGEQGDDGK 814          

HSP 20 Score: 51.9878 bits (123), Expect = 7.749e-6
Identity = 45/112 (40.18%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKG---VKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKD---------GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G RG+ G  G +GD GP G  G  G  GM G     G+ G+ G  GP+G AG  G  G+QG  GP+G+          G +G  G +G PG  G+ G KG  G  G QG  G
Sbjct:  904 GPRGVIGREGFEGDPGPDGAAGKDGAKGMPGDLGHDGDVGLPGKPGPQGNAGAPGLPGVQGSFGPKGERGIPGQSGPPGKRGLNGGMGFPGKQGDQGFKGQPGDAGEQGFPG 1015          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Zebrafish
Match: si:dkey-21p1.3 (si:dkey-21p1.3 [Source:ZFIN;Acc:ZDB-GENE-081105-162])

HSP 1 Score: 155.992 bits (393), Expect = 1.178e-37
Identity = 285/890 (32.02%), Postives = 383/890 (43.03%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGT------------------RGEVGIKGSEGKTGLMGSRGPQ---GVSGEPGSPGD---------AGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGEL-------GIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXX--XXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPI------GIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVK--IRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQ-GGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGC----MYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            G RGP GL GKPG  G +G IG KG+ G RG +G PG  GI G +G+ G  G  G  G++GK GP+GP G  G+                G  G  G  G+ G +G+ G+ GP G  G  GV G  G+ G +GPIGP+G+KG+ G  G +GLKG                    G  GI+G  G  GL G +GP+   G+SG PG  GD          GV G+ G  G+ G  G  G  G +G  G PG  GE G  G IG  G  G PG  G+ G  G++G  G +G+PG+KG+ G  G +G  G  GL   +G  G  G +G  G  G+ G+ G  GP+G +G L       G +G PGR+G  G  G PG  GT G  G  G  G P      +   +G +G  G PG+ GLQG AG +G  G  G RG  GP G  G  G+ GP+GPI      G+ G  G  GQ G +G +G  G  GP G  G+ G  G PG KG    KGIKG  G  G  G KG PG PG+ G QGK G  G KG+ G  G  G V +                                          VK     PP+    + K  +R   F + +D + +                   KD+ A +C ++ L +    DG YY+DPN G   D++ V C F+    TC+S V+    G++         ++   S      + +   L    +D  Q+ FLR  ++ + Q I   C +            N +K    L    ++ I+                            +V I   GC      R  GSS   +L   D+ +LPI D  +            + +  +CF
Sbjct:  350 GHRGPRGLQGKPGAPGIQGPIGKKGETGSRGLTGKPGKPGISGVKGKEGSVGLHGKPGEKGKIGPEGPKGKPGQ---------------PGLQGNIGQWGETGVRGLEGHQGPPGLPGPHGVAGYPGKDGPLGPIGPEGDKGNKGLRGDSGLKGVAGIDGEEGEKGKEGSVGDHGPKGIQGESGNQGLSGVKGPKGRDGMSGAPGQKGDPGSSGEKGHPGVRGQSGLVGQYGPGGNMGEGGDKGDKGLPGLAGETGTTGPIGPSGVIGLPGLTGKEGFGGQSGKHGPKGEPGLKGSSGGPGKIGSVGAIGL---IGKKGEPGKQGRVGHVGQPGAVGPQGPKGKQGSLGAPGKKGGTRGFPGRRGSKGVAGKPGRSGTDGLLGVIGTEGDPNDILDDRFSTEGDIGFRGRPGRPGLQGSAGATGIPGLQGSRGPAGPKGSIGLKGKTGPSGPIGPKGIPGLQGKKGDDGQKGTMGQVGITGLQGPIGPPGVKGNQGTPGYKGRPGPKGIKGESGARGRPGGKGLPGSPGIKGVQGKSGQKGVKGQRGRPGKPGIVSQ------------------------------------------VKPGPHTPPQRRGSQNKALSRMLRFDNFDDWIEENSHLQG------------TKDNPASSCRELSLIQPHLRDGHYYVDPNHGCPFDALRVFCNFTAGGLTCVSPVQSNISGVW--------PSSVDRSKMWFSELHKGFKLKYKDLDVVQLRFLRLHSNSATQSISLKCPK------KHSPTVNHWKTMLHLRGDSYEEINPP--------------------------HVIITRHGCEVTAQVRVHGSS---ELHRGDMQLLPIRDVFVEGGEETWKGHANIILGHLCF 1124          

HSP 2 Score: 84.7297 bits (208), Expect = 7.708e-16
Identity = 194/556 (34.89%), Postives = 248/556 (44.60%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMG------SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPG------------------------VKGTKGDTGILGP------RGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGS------KGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G+ GP+GLPG  G DG  G  G +G  GP+G+ G+PG QG+ G RG  G  G+ G  G  G  G  G +GD+G  G+PG +G PG  G  G                            KG +GL G  GS GP G  G  G  G LG  G +G RG  G++G  G  G+ G        G +G++G+PG PG +GV                                 KG +G +GL G  G  G+IG  GP GK G  G +G  G                        V G  G  G LGP      +G +GL G  G  G  G+ G  G+ G++GS G+ GP+G++GE G QG  G KG  G  G+ G  G                 G  G  G  G PG+ G++GL G  G  G  GE G++GD+G PG+AG  G  GP GP G++                  G PG  G  G  G+ G+ G KGE  LKG  G PG IG+VG+      KGEPG  G  G  G+ G  GP+G  G  G +G  G+ G
Sbjct:  224 GSNGPAGLPGPTGNDGFIGEPGRQGQDGPKGERGSPGIQGVPGPRGDKGRIGQGGFSGFPGPLGEMGESGDQGPEGEPGIKGRPGVPGGTG---------------------------SKGRKGLQGVSGSRGPDGTQGAHGSTGLLGSNGDRGHRGPRGLQGKPGAPGIQGPIGKKGETGSRGLTGKPGKPGISGV---------------------------------KGKEGSVGLHGKPGEKGKIGPEGPKGKPGQPGLQGNIGQWGETGVRGLEGHQGPPGLPGPHGVAGYPGKDGPLGPIGPEGDKGNKGLRGDSGLKGVAGIDGEEGEKGKEGSVGDHGPKGIQGESGNQGLSGVKGPKGRDGMSGAPGQ---------------KGDPGSSGEKGHPGVRGQSGLVGQYGPGGNMGEGGDKGDKGLPGLAGETGTTGPIGPSGVI------------------GLPGLTGKEGFGGQSGKHGPKGEPGLKGSSGGPGKIGSVGAIGLIGKKGEPGKQGRVGHVGQPGAVGPQGPKGKQGSLGAPGKKG 686          

HSP 3 Score: 79.7221 bits (195), Expect = 3.165e-14
Identity = 190/562 (33.81%), Postives = 220/562 (39.15%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGP------------QGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLM---GSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQP------GVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAG---------NMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN------------------PGPKGETGLMGPIGPVG 4030
            G PG  GPSG PG     G +G  G KGD   +G +G PG QG RG  GQSG  GE GS G  G  GP            QG  G KGER                              G  G  G  GP G+KG  G  G  G  GP+G  GE GD GP G+ G+KG  G  G  GS+G+ GL    GSRGP G  G  GS G  G N                              G++G +G  GL G  GAPG  G  G  G+TG+ G  G+P      GVKG +G  G+ G  GE+G                          G  GP+G  G+ G+QG  G+ GE G  GL                   G  G  G  GP G  G PGK G  GP G  G +G  G RGD G  G+AG           G  G +GP GI                           +G  G  G  G KG +   G+ G+PG  G  GS GE G PG+ G  G  G                   PG  GETG  GPIGP G
Sbjct:  170 GKPGNLGPSGDPGLKARRGMKGESGQKGD---QGAAGLPGKQGFRGRGGQSGVLGETGSNGPAGLPGPTGNDGFIGEPGRQGQDGPKGER------------------------------GSPGIQGVPGPRGDKGRIGQGGFSGFPGPLGEMGESGDQGPEGEPGIKGRPGVPGGTGSKGRKGLQGVSGSRGPDGTQGAHGSTGLLGSN------------------------------GDRGHRGPRGLQGKPGAPGIQGPIGKKGETGSRGLTGKPGKPGISGVKGKEGSVGLHGKPGEKG------------------------KIGPEGPKGKPGQPGLQGNIGQWGETGVRGL------------------EGHQGPPGLPGPHGVAGYPGKDGPLGPIGPEGDKGNKGLRGDSGLKGVAGIDGEEGEKGKEGSVGDHGPKGI---------------------------QGESGNQGLSGVKGPKGRDGMSGAPGQKGDPGSSGEKGHPGVRGQSGLVGQYGPGGNMGEGGDKGDKGLPGLAGETGTTGPIGPSG 599          

HSP 4 Score: 73.1738 bits (178), Expect = 2.619e-12
Identity = 202/598 (33.78%), Postives = 251/598 (41.97%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGP------------QGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL------GKTGLKGT---RGEVGIKGSE------------------GKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEK---GVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQ------------------------PGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGER-------GDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXS-----RGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGE------TGLMGPIGPVGEDG 4039
            G +GP G PG  G  G  G  G KG  G +G SG+ GP G +GA G    TG LGS GDRG  GP            QGP G KGE                                  G+ G  G  G+ G+ G+ G++GSVG  G  GEKG +GP       G+ GL+G     GE G++G E                  GK G +G  GP+G  G  G  GD+G+ G  G  G++G +G  G VG  G  G  G  G +   GVKG  G  G +GAPG+ G+ G  G+ G  G  GQ                        PG+ G  G TG +GP G  GL             G  GK G  G +G  GP+G  G  G  G PG+ G +G IGL G  G  G  G+ G                    G PG  G QGP G  G+ G  G++       G RG  G+AG  G+ G +G +G++G +G                           +G  G  G PG +G R   G KGS G  G  G  G  GP G+ G QGK+G+ G KG       TGL GPIGP G  G
Sbjct:  293 GDQGPEGEPGIKGRPGVPGGTGSKGRKGLQGVSGSRGPDGTQGAHGS---TGLLGSNGDRGHRGPRGLQGKPGAPGIQGPIGKKGE---------------------------------TGSRGLTGKPGKPGISGVKGKEGSVGLHGKPGEKGKIGPEGPKGKPGQPGLQGNIGQWGETGVRGLEGHQGPPGLPGPHGVAGYPGKDGPLGPIGPEGDKGNKGLRGDSGLKGVAGIDGEEGEKGKEGSVGDHGPKGIQGESGNQGLSGVKGPKGRDGMSGAPGQKGDPGSSGEKGHPGVRGQSGLVGQYGPGGNMGEGGDKGDKGLPGLAGETGTTGPIGPSGVIGLP------------GLTGKEGFGGQSGKHGPKGEPGLKGSSGGPGKIGSVGAIGLIGKKGEPGKQGRVGHV------------------GQPGAVGPQGPKGKQGSLGAPGKKGGTRGFPGRRGSKGVAGKPGRSGTDGLLGVIGTEGDPNDILDDRFSTEGDIGFRGRPGRPGLQGSAGATGIPGLQGSRGPAGPKGSIGLKGKTGPSGPIGPKGIPGLQGKKGDDGQKGTMGQVGITGLQGPIGPPGVKG 824          

HSP 5 Score: 69.3218 bits (168), Expect = 3.789e-11
Identity = 133/433 (30.72%), Postives = 158/433 (36.49%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGA------RGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKG-----------TRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGP 3736
            GP G  G  GL GK G  G+ G  GPKG+ G +G SG PG  G  GA      +G+ G  G +G  G  G  GPQGP G +G                                          P  + G RG  GR+GS G  G  G  G  G LG  G +G           T G++G +G  G+ GL GS G  G+ G  GS G A                                    G KG IGL G TG  G IG  G PG  G  G +GQ G  G  G TG+ GP G  G+                            G QG  G  G  GP G KGE G  G PG                                GLPG  G++G  G SG +G  G+RG  G PGI   + + GP+ P
Sbjct:  596 GPSGVIGLPGLTGKEGFGGQSGKHGPKGEPGLKGSSGGPGKIGSVGAIGLIGKKGEPGKQGRVGHVGQPGAVGPQGPKGKQGS--------------------------------------LGAPGKKGGTRGFPGRRGSKGVAGKPGRSGTDGLLGVIGTEGDPNDILDDRFSTEGDIGFRGRPGRPGLQGSAGATGIPGLQGSRGPA------------------------------------GPKGSIGLKGKTGPSGPIGPKGIPGLQGKKGDDGQKGTMGQVGITGLQGPIGPPGV---------------------------KGNQGTPGYKGRPGPKGIKGESGARGRPG------------------------------GKGLPGSPGIKGVQGKSGQKGVKGQRGRPGKPGIVSQV-KPGPHTP 896          

HSP 6 Score: 61.2326 bits (147), Expect = 1.130e-8
Identity = 132/401 (32.92%), Postives = 162/401 (40.40%), Query Frame = 2
Query: 2864 RGLLGRKGSVGPIGPD------GEKGDVGPLGKTGLK---GTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGD------TGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            RG +G +GS+G  GP       G+ G++GP G  GLK   G +GE G KG +G  GL G +G +G  G+ G  G+ G N                              G  G+ G  G  G  G PGR G+ GP G+ G+ G +G PG +G KG       +G  GP GE G                            SG QG EGE GI+G PG  G  G  G  G  G S                           G  G  G QG  G +G  G  G+RG RGP G+ G  G  G  GPIG                       G  GSRGL GK G+PG      + G+KG  G++G  G  GE G  G  GP+GK G PG +G  G  G  G
Sbjct:  148 RGDVGIEGSIGGPGPPGIKGAIGKPGNLGPSGDPGLKARRGMKGESGQKGDQGAAGLPGKQGFRGRGGQSGVLGETGSN------------------------------GPAGLPGPTGNDGFIGEPGRQGQDGPKGERGSPGIQGVPGPRGDKGRIGQGGFSGFPGPLGEMG---------------------------ESGDQGPEGEPGIKGRPGVPGGTGSKGRKGLQGVS---------------------------GSRGPDGTQGAHGSTGLLGSNGDRGHRGPRGLQGKPGAPGIQGPIG---------------------KKGETGSRGLTGKPGKPG------ISGVKGKEGSVGLHGKPGEKGKIGPEGPKGKPGQPGLQGNIGQWGETG 437          

HSP 7 Score: 57.7658 bits (138), Expect = 1.577e-7
Identity = 76/231 (32.90%), Postives = 95/231 (41.13%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIG-----------------PKGDLGPRGQSGNPGPQGIRGA------RGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGE------------PGSPGDAGVN 3073
            RG PG RG  G+ GKPG  G +G +G                  +GD+G RG+ G PG QG  GA      +G  GP G  GS G +GK GP GP G K                                                G+ GL G+KG       DG+KG +G +G TGL+G  G  G+KG++G  G  G  GP+G+ GE            PGSPG  GV 
Sbjct:  689 RGFPGRRGSKGVAGKPGRSGTDGLLGVIGTEGDPNDILDDRFSTEGDIGFRGRPGRPGLQGSAGATGIPGLQGSRGPAGPKGSIGLKGKTGPSGPIGPK------------------------------------------------GIPGLQGKKGD------DGQKGTMGQVGITGLQGPIGPPGVKGNQGTPGYKGRPGPKGIKGESGARGRPGGKGLPGSPGIKGVQ 865          

HSP 8 Score: 56.6102 bits (135), Expect = 2.898e-7
Identity = 47/105 (44.76%), Postives = 61/105 (58.10%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGV---KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA GI G  G++G  GP G +G +G+ G  G    KG  G+QG +G +G  G  G +GI G  GP G  G KG++G+   PGY G PG KG +G  G +GR G K
Sbjct:  752 GATGIPGLQGSRGPAGPKGSIGLKGKTGPSGPIGPKGIPGLQGKKGDDGQKGTMGQVGITGLQGPIGPPGVKGNQGT---PGYKGRPGPKGIKGESGARGRPGGK 853          

HSP 9 Score: 56.6102 bits (135), Expect = 3.101e-7
Identity = 46/115 (40.00%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGE------------YGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G +GS G KG  GPSG +G +G  G++G KG+             G+QGP GP G+ G +G  G +G  GP+G  G  G  G  G  G  G+PGIKG +G  G +G  G++
Sbjct:  763 RGPAGPKGSIGLKGKTGPSGPIGPKGIPGLQGKKGDDGQKGTMGQVGITGLQGPIGPPGVKGNQGTPGYKGRPGPKGIKGESGARGRPGGKGLPGSPGIKGVQGKSGQKGVKGQR 877          

HSP 10 Score: 55.0694 bits (131), Expect = 1.003e-6
Identity = 45/102 (44.12%), Postives = 59/102 (57.84%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGN---PGIKGSRGPVGNQGRDGEK 2137
            G++GS+GA G  G  G  G  G  G  G+KG+ G  GP GP+GI G++G  G  G+ G  G+ G  G +G +GPPG  GN   PG KG  GP G +G  G +
Sbjct:  746 GLQGSAGATGIPGLQGSRGPAGPKGSIGLKGKTGPSGPIGPKGIPGLQGKKGDDGQKGTMGQVGITGLQGPIGPPGVKGNQGTPGYKGRPGPKGIKGESGAR 847          

HSP 11 Score: 54.299 bits (129), Expect = 1.597e-6
Identity = 49/118 (41.53%), Postives = 65/118 (55.08%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPS---GQVGTRGEVGMKGVKGEYGIQGPR---GPEGIAGVKGDIGIQGETGPRGKDGN---------KGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
             K  RG++G SG KGD G +   G+ G RG  G  GV GE G  GP    GP G  G  G+ G QG+ GP+G+ G+         +GD+G +G  G++G PG  G  G  G+QG +GE
Sbjct:  183 LKARRGMKGESGQKGDQGAAGLPGKQGFRGRGGQSGVLGETGSNGPAGLPGPTGNDGFIGEPGRQGQDGPKGERGSPGIQGVPGPRGDKGRIGQGGFSGFPGPLGEMGESGDQGPEGE 300          

HSP 12 Score: 53.1434 bits (126), Expect = 4.183e-6
Identity = 47/113 (41.59%), Postives = 60/113 (53.10%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG---IAGVKGDIGIQGETGPRGKDGN---------KGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G++G +G  G  G  GP G  G  G  G+ G  G+ G +GPRG +G     G++G IG +GETG RG  G          KG EGSVG     G PG KG  GP G +G+ G+
Sbjct:  314 GSKGRKGLQGVSGSRGPDGTQGAHGSTGLLGSNGDRGHRGPRGLQGKPGAPGIQGPIGKKGETGSRGLTGKPGKPGISGVKGKEGSVG---LHGKPGEKGKIGPEGPKGKPGQ 423          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Zebrafish
Match: col1a2 (collagen, type I, alpha 2 [Source:ZFIN;Acc:ZDB-GENE-030131-8415])

HSP 1 Score: 130.183 bits (326), Expect = 1.317e-29
Identity = 293/903 (32.45%), Postives = 371/903 (41.09%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQ------------------SGNPGPQGIRGARGQSGPTGELGSKG------DRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKG---------------------SEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGE------------AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITN-KIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPM--KGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLE--TTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            GP G  G +G PG  GI G +G  GP G  G RG                   SG PGP G +G  G  GP G  G +G      +RG  G  G  G+KGE G  G +G  G  G  G  G +G PG  G  G  G  G  GP G  G RG  G +G  GP GP G  G  G  G+TG +G +G  G KG                       G  G  G  GP G++G PG+PG                                      G  G  G++GP G        GP GK G  G  G  G  G  G+ G++GP G  G  GP G  G+ G  G  G             QG  G  G  G PG +G+ G  G+PG VG  G  G  GAPG  G  G           G+PG  G QG AG  G+ G              G+RG+ G PG AG +G  GPNGP G V         G  G  G  G  GP G+ G  G  G PG  G R  KG+ G  G  G  G +G PG  G+ GP G  G+ GP G  G  GP GP G +G +G  G  G  G  GPPG RG  G  G  G  G PG PG          +SG Y      Q+ +   D   D   K  N +I+ +  P G  K+  ARTC DI+L   + + G Y+IDPN+G   D+I+  C FS   +TCI    +     N+ +    K  T +  ++      +   +  S     TQ+ F+R   +++ Q I + C  S+ Y D   +E    K A +L                   +G  D  +  +   R  F  ++  DGC   T G  SK  +E  T   S LPI+D    D         GL++  +CF
Sbjct:  549 GPAGPVGEAGKPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGKDGARGAPGPSGPPGPAGANGDKGETGSFGPPGPAGPRGAPGERGESGPAGPSGFAGPPGADGQTGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRV------------------------------------GPPGPAGIVGPAGL------TGPAGKDGPRGPRGDVGPAGPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGP------------QGQLGSQGFNGLPGSRGDRGLPGIPGSVGEPGRVGPAGAPGARGPGGNI---------GMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGTAGPVGAPGPNGPSGAV---------GRPGNRGESGPSGPTGAVGPAGARGAPGPAGPRGEKGVAGEKGDRGMKGLRGHPGLQGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDGSNGMPGAIGPPGHRGPAGHVGPAGPPGSPGLPGPPGPSGGGYDTSGGYDEYRADQASLRAKDYEVDATIKSLNTQIENLLSPEGSKKNP-ARTCRDIRLSHPEWSSGFYWIDPNQGCTMDAIKAFCDFSTG-QTCIHPHPESIPRKNWYRSSQEKKHTWFGETINSGTEFAYNDETLSPQSMATQLAFMRLLANQAVQNITYHCKNSIAYMD---AENGNLKKAVLL-------------------QGSNDVELRAEGNSR--FTFSVLEDGCSRHT-GQWSKTVIEYRTNKPSRLPILDIAPLDIGGA-DQEFGLDIGPVCF 1351          

HSP 2 Score: 84.3445 bits (207), Expect = 1.359e-15
Identity = 233/571 (40.81%), Postives = 282/571 (49.39%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQG---IRGARGQSGPTGELGSKGDRGKFGPQG---PTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVG------PLGKTGLK---------------GTRGEVGI---------------KGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGE------------AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGE---PGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G  GP+GL G PG DG  G  GP G  G  G+ G  GP G    +G  G +GP GE G  GDRG  G QG   P G KGERG+PG  G  G  GP G  G +G+PG  G KG  G  GP G  G +G  G+ G +G+ G  G  GEKG+ G        GK G +               G +GE G                +G  G  G  G  GP G  G+ G  G+ G  G KG+ G  G  GP G  G  G  G  GP G +G  G  GL G  GAPGR+G  GP G  G +G  G  G  G +G  G +GP G  G +G +GP+G  G KG  G+ G  G+ G +GPQG  G  G  G PG +G+ G  G+PG VG  G  G  GAPG  G  G           G+PG  G QG AG  G+ G              G+RG+ G PG AG +G  GPNGP G VG  G RG+ GP GP G +G  G +G+ G  G  GE    G+KG+R +KG++G PG       +G PGP G +G  G  G  GP G  G  GP GP G+DG
Sbjct:  501 GPVGPTGLRGSPGPDGNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEAGKPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGKDGARGAPGPSGPPGPAGANGDKGETGSFGPPGPAGPRGAPGERGESGPAGPSGFAGPPGADGQTGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRVGPPGPAGIVGPAGLTGPAGKDGPRGPRGDVGPAGPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGPQGQLGSQGFNGLPGSRGDRGLPGIPGSVGEPGRVGPAGAPGARGPGGNI---------GMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGTAGPVGAPGPNGPSGAVGRPGNRGESGPSGPTGAVGPAGARGAPGPAGPRGEKGVAGEKGDRGMKGLRGHPGL------QGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDG 1056          

HSP 3 Score: 74.3294 bits (181), Expect = 1.370e-12
Identity = 235/584 (40.24%), Postives = 282/584 (48.29%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKG------DRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPI------GPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGS---PGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGV---KGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXN---------------SGPQGVEGELGIQGPPGRKGELGPIGLPGPVG---------------TXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGK------AGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP---------GPPGLTGPQ---GKRGNPGPKGETGLMGPIGPVGEDGE 4042
            GP G RGP G  G  G+ G  G  GP G  GPRG  G PG +G  G  G++G  G +G++G        G  G +GP+G  G+                   G  G PG  G +G  GN G  GP G  G  G  G KG VGP       GPDG  G  GP+G  G  G +GE G  G+ G  GL G  GP G +G+PG    PGD GV+G  G +G++G+ GP G  G QG  G  GP G  G    KG+ G +GP GAPG  G AG PG+ GA+G    PG KG KG+ G  G  G  G DG  G  G  G  G  G  G +G TG+                G  G  G  G  GPPG  G+ GP G  GP G                +GP G  G  GPPG  G +G  G  G PG PG+      AG+ GPAG +G  G+ G RG RG  G AG  G+ G  GP+G+ G  GP G+ G  G  GP G  G  GS+G +G    PG +G+R L GI GS G  G VG  G P         G PG+TGPQ   G+ G+PG  G  G  G  G  G+ GE
Sbjct:  381 GPLGLRGPRGAAGTRGLPGLAGRSGPMGMPGPRGGVGAPGARGPPGDAGRAGEAGLVGARGLPGSPGSSGPPGKEGPSGAAGQ------------------DGRTGPPGPTGPRGQPGNIGFPGPKGPSGEAGKPGEKGPVGPTGLRGSPGPDGNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEAGKPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGT---PGAKGEKGEAGYRGLEGNAGKDGARGAPGPSGPPGPAGANGDKGETGSFGPPGPAGPRGAPGERGESGPAGPSGFAGPPGADGQTGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRVGPPGPAGIVGPAGLTGPAGKDGPRGPRGDVGPAGPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGPQGQLGSQGFNGL---PGSRGDRGLPGIPGSVGEPGRVGPAGAPGARGPGGNIGMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGE 940          

HSP 4 Score: 70.4774 bits (171), Expect = 2.258e-11
Identity = 209/566 (36.93%), Postives = 254/566 (44.88%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDV---------------GPLGKTGLKGTRGE------VGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQ---GPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ---------------------------GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            PG +GPSG  GKPG  G  G  G +G  GP G +G  GP G+ GA G+ G  G  G+ G +G  GP GP G+ G                                             + G RG+ G +G  GP G  GE+G+                GP G  G  G +GE       G  G +G  G+ G RG  G  G  G  G+AG  G +G  GK G++G  GP G  G  G  G +GE G  G  G  GP GAPG  GE+GP G +G +G  G  G  G +G+ G  G +G+ G  GP GP G+ G  G  G  G  G+ G+SGP G+ G  G     GPPG  G +GP GL GP G  GP G  G  GP                G PG+ G+ GP G +G +                           G  G RGDRG PGI G++G+ G  GP G  G  GP G  G  G  GP G  G +GS G DG  G PG  G +  +G  GSPGT G VG+ G  GP G  G  G RG  GP G TG +GP G  G  G
Sbjct:  485 PGPKGPSGEAGKPGEKGPVGPTGLRGSPGPDGNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEAG---------------------------------------------KPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGKDGARGAPGPSGPPGPAGANGDKGETGSFGPPGPAGPRGAPGERGESGPAGPSGFAGPPGADGQTGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRVGPPGPAGIVGPAGLTGPAGKDGPRGPRGDVGPA---------------GPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGPQGQLGSQGFNGLPGSRGDRGLPGIPGSVGEPGRVGPAGAPGARGPGGNIGMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGTAGPVGAPGPNGPSGAVGRPGNRGESGPSGPTGAVGPAGARGAPG 990          

HSP 5 Score: 58.151 bits (139), Expect = 1.267e-7
Identity = 157/451 (34.81%), Postives = 174/451 (38.58%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRG------------------ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEV------------GIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIV 3748
            RG  G  GPSG  G PG DG+    GP+G+ GP G  G+ GP G  G                  ARG SGPTG  G  G  G+ GP GP G  G                                         G  G  G  G  G +G VGP GP GE G +GPLG  G KG  GE             G  GS+G  GL GSRG +G+ G PGS G+ G                 GP G  G  G  G  G  G+ G  G  G  G+PG  G  G PG  G  G  G+PG  GT G  G  GP G  G                             G  G  GE G  GP G  G  G  G PGP G        G  G  G  G  G  G  G PGL G  G  GP+G SG  G AG  G RGP G  G  G+ G NG  G +
Sbjct:  704 RGESGPAGPSGFAGPPGADGQ---TGPRGEKGPAGGKGDAGPAGPAGPAGNTGPLGPSGPVGPPGARGDSGPTGLTGFPGAPGRVGPPGPAGIVGP---------------------------------------AGLTGPAGKDGPRGPRGDVGPAGPPGENGMIGPLGLAGEKGPPGEAGAPGAPGPAGPQGQLGSQGFNGLPGSRGDRGLPGIPGSVGEPG---------------RVGPAGAPGARGPGGNIGMPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGTAGPVGAPGPNGPSGA---------------------------VGRPGNRGESGPSGPTGAVGPAGARGAPGPAGP------RGEKGVAGEKGDRGMKGLRGHPGLQGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDGSNGMPGAI 1064          

HSP 6 Score: 57.7658 bits (138), Expect = 1.333e-7
Identity = 198/557 (35.55%), Postives = 231/557 (41.47%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQ---------------------GPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRG------------------PNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGE---TGLMGPIGPVGEDGE 4042
            G RGPSG PG PG  G +G  G  G+ G  G  G  GP G  G  G+ G  G  G  GDRG  G QG  G  G                                     PG  G  G +G  G+ GRKG  G  G  GE G  G  G  G +G RG  G +G  G  G  G+RG  G +G  G  G  G                       G PG PG  G KG  G  G  GP+GA G+ GE GP G  G  G  G PG  G  G  G  G  G  G  G  GP G  G +G  G  G +G  G+ GP GV+G+ G++                     G  G +G  GP+GL GP G +G                          GLPG AG  GP G  G +G  G  G RGPPG AG  G+ G                    GP G  G DG  G PGP GP G  GN G  G +G  G+ G+PG+KG     G++GSPG     G+ G  GP GL G  G++G  GP G     GL GP GPVGE G+
Sbjct:   87 GPRGPSGSPGAPGAQGLQGHAGEPGEPGQAGAIGARGPPGPPGKNGEDGNNGRPGKPGDRGVLGAQGARGFPGT------------------------------------PGLPGMKGHRGYNGIDGRKGEPGAAGAKGENGAAGSNGTPGQRGGRGLPGERGRVGPAGPAGARGADGNTGPAGPAGPLG---------------------SAGPPGFPGAPGPKGELGPAGPTGPSGAQGQRGEPGPNGAVGPVGPPGNPGANGINGAKGAAGLPGIAGAPGFPGPRGGPGPQGPSGASGPRGLGGDPGPVGVKGDSGVKGEPGSAGPQGPPGPSGEEGKRGSTGEQGPTGPLGLRGPRGAAGTR------------------------GLPGLAGRSGPMGMPGPRGGVGAPGARGPPGDAGRAGEAGLVGARGLPGSPGSSGPPGKEGPSGAAGQDGRTGPPGPTGPRGQPGNIGFPGPKGPSGEAGKPGEKGPVGPTGLRGSPGPD---GNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEAGK 559          

HSP 7 Score: 56.225 bits (134), Expect = 3.957e-7
Identity = 40/91 (43.96%), Postives = 52/91 (57.14%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRG---------KDGNKGDEGSVGPPGYAG 2077
            G  G RG+ G  G  G  G  G +G+ GMKG++G  G+QG  GP G +G  G  GI G +GPRG         KDG+ G  G++GPPG+ G
Sbjct:  981 GPAGARGAPGPAGPRGEKGVAGEKGDRGMKGLRGHPGLQGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDGSNGMPGAIGPPGHRG 1071          

HSP 8 Score: 53.9138 bits (128), Expect = 2.193e-6
Identity = 201/569 (35.33%), Postives = 243/569 (42.71%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRG---ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEV---GIKGSEGKTGLMG---------------------SRGPQGVSGEPGS---PGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGK------TGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGK---VGEPGQKGER------------YLKGIKGSPGTIGAVGSKGEP---GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            PG +G  G  G  G  G+ G+ G KG+ G  G +G PG +G RG    RG+ GP G  G++G  G  GP GP G  G  G PG  G PGP G                                     LG  G  GP G  G++G+ GP G  G  G  G     GI G++G  GL G                     + GP+G+ G+PG     GD+GV GE G  G QG  GP+G  G +G  G+ GP G  G++G  G  G  G PG  G +GP G        GA GA G PG  G  G+ G++G RG  G  G  GP G +G  G  G+ GR G  G +GP+G  G +G  GP G  GE G  G  GPVG +G                          G PG  G  GPAG  G  G  GE+G++GP G  G  G  GP GP+G                       G  G RG+ G     G  G KGER               G +G  GT G  G+KGEP   GP G  GPQG  G PG +G  G  G  G  GE G
Sbjct:  164 PGMKGHRGYNGIDGRKGEPGAAGAKGENGAAGSNGTPGQRGGRGLPGERGRVGPAGPAGARGADGNTGPAGPAGPLGSAGPPGFPGAPGPKGE------------------------------------LGPAGPTGPSGAQGQRGEPGPNGAVGPVGPPGNPGANGINGAKGAAGLPGIAGAPGFPGPRGGPGPQGPSGASGPRGLGGDPGPVGVKGDSGVKGEPGSAGPQGPPGPSGEEGKRGSTGEQGPTGPLGLRGPRGAAGTRGLPGLAGRSGPMGMPGPRGGVGAPGARGPPGDAGRAGEAGLVGARGLPGSPGSSGPPGKEGPSGAAGQDGRTGPPGPTGPRGQPGNIGFPGPKGPSGEAGKPGEKGPVGPTGLR------------------------GSPGPDGNNGPAGPVGLAGAPGEKGEQGPSGAPGFQGLPGPAGPVGEA---------------------GKPGDRGIPGDQGVSGPAGVKGERGNPGPAGAAGAQGPIGARGPSGTPGPDGNKGEPGAVGPAGAPGPQGAAGMPGERGAAGTPGAKGEKGEAG 651          

HSP 9 Score: 51.9878 bits (123), Expect = 7.514e-6
Identity = 45/103 (43.69%), Postives = 55/103 (53.40%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGD---IGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G  G  G+ G  G+ G SG  G  G VG  G +G  G  GPRG +G+AG KGD    G++G  G +G  G  G  G  GP G AG  G +G  GP G  G+DG
Sbjct:  954 GPNGPSGAVGRPGNRGESGPSGPTGAVGPAGARGAPGPAGPRGEKGVAGEKGDRGMKGLRGHPGLQGMPGPNGPSGDSGPAGIAGPSGPRGPAGPNGPAGKDG 1056          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Zebrafish
Match: col11a1a (collagen, type XI, alpha 1a [Source:ZFIN;Acc:ZDB-GENE-070209-167])

HSP 1 Score: 129.413 bits (324), Expect = 1.979e-29
Identity = 122/475 (25.68%), Postives = 191/475 (40.21%), Query Frame = 2
Query: 3605 GLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDAL---FDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            G PGK G  GP G SG  G  G RG  GP    G+ G  GQ GP GP+              +            G  GL G +G PG++GE   KG +G PG  GA GSKG+ GP    GP+G +G+ GP G+ G  G +                                               + I +P +       ++  A + +D  + +   F  ++ +   I+R+++P G  +++ AR+C D++L   +  DG+YYIDPN+G   DS +V C F+   +TCI   K+  G+     P +   +W +  +  G I    D     ++  QM FL+   + + Q + + C QSV + D+     ++         H   S D          E   D   Y          +    DGC  R     +  ++ T  +  +PI+D +  D+ +      G  V  +CF
Sbjct:  989 GPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPM--------------VREKIMFLLFFLSGHPGLIGLIGPPGEQGE---KGDRGLPGPQGAAGSKGDNGP---QGPKGSKGSTGPAGQKGDTGTV--------------------------------GPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMG-TQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFK-RGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDR-------ALHFLGSND---------EEISYDNNPY----------IKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPN-QKFGFEVGPVCF 1382          

HSP 2 Score: 105.916 bits (263), Expect = 2.946e-22
Identity = 64/218 (29.36%), Postives = 98/218 (44.95%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRRK----AFRYESGNSLMAKSDVLF-QGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
            VD+L  ++      G      FC N R      A++      L A +  LF  G FP++FSIL T K +A     + SI N+     L V  GR     Y D    P+   +  L    +L DG+WHR+  SV+K +V++++DC+   +  L R+      + G          + SF G +  LL+V     A+  C+ Y+PDC+    D +  Q P
Sbjct:   25 VDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDY-PLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEP 241          

HSP 3 Score: 62.003 bits (149), Expect = 8.563e-9
Identity = 47/108 (43.52%), Postives = 63/108 (58.33%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQV------GTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            Y G RG++G+ G +G  G  G+       G +G++G+KG +GE G+ GPRG +G  G KG  G  GE+GP+G  G KG  G  G PGY G  G KG  G  G +G+ G
Sbjct:  596 YPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGVAGKAGPRGQRG 703          

HSP 4 Score: 58.151 bits (139), Expect = 1.103e-7
Identity = 109/299 (36.45%), Postives = 125/299 (41.81%), Query Frame = 2
Query: 3170 KGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGE---------------------LGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG 3994
            KG  GD G  GP    G PG+IG+ G  G+ GA GA G PG  G+KGD G  G  G  G  G  G  G  G  G  G+ G +G  G  GP+G+ GE                        QG PG +G   PIG PGP G  G  G  G  G  G  G+ G       PG  G  GL+G  G  G  G  GE G  GP G AG  G+ GP G                              S G  GK+G PG  G    +G KG  G  G  G +G  GP G  G +G  G PGPKG
Sbjct:  465 KGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESYLGHRFSCALHHKHLIIFHCCHFQGQPGPQG---PIGYPGPRGVKGADGVRGLKGGKGEKGEDGF------PGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQG------------------------------SAGEKGKLGVPGLPGYPGRQGPKGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKG 724          

HSP 5 Score: 55.0694 bits (131), Expect = 9.802e-7
Identity = 116/293 (39.59%), Postives = 133/293 (45.39%), Query Frame = 2
Query: 2882 KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPG------RIGEAGPPGKT---GASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNG 3733
            KG  G  GP G +G  GP G+ G  G RG  G  G+ G  G  GS+G +G  G PG PG+ G                    G  G  G PGP GE G +G+ G IGP G  G      R   A              +GQP   G +G  G  GPRG +G D   G  G KG KG +G+ G  G  G+ G +G  GELG+ GP G  G  GP G  GP G SGP G  G  G  G             PGLPG  G QGP    G  G+AG RG RGP G  G  G RGP G
Sbjct:  465 KGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKG------------------HRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESYLGHRFSCALHHKHLIIFHCCHFQGQP---GPQGPIGYPGPRGVKGAD---GVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGV------------PGLPGYPGRQGP---KGVAGKAGPRGQRGPTGPRGARGARGPTG 718          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Zebrafish
Match: col11a1a (collagen, type XI, alpha 1a [Source:ZFIN;Acc:ZDB-GENE-070209-167])

HSP 1 Score: 129.413 bits (324), Expect = 2.040e-29
Identity = 122/475 (25.68%), Postives = 191/475 (40.21%), Query Frame = 2
Query: 3605 GLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDAL---FDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            G PGK G  GP G SG  G  G RG  GP    G+ G  GQ GP GP+              +            G  GL G +G PG++GE   KG +G PG  GA GSKG+ GP    GP+G +G+ GP G+ G  G +                                               + I +P +       ++  A + +D  + +   F  ++ +   I+R+++P G  +++ AR+C D++L   +  DG+YYIDPN+G   DS +V C F+   +TCI   K+  G+     P +   +W +  +  G I    D     ++  QM FL+   + + Q + + C QSV + D+     ++         H   S D          E   D   Y          +    DGC  R     +  ++ T  +  +PI+D +  D+ +      G  V  +CF
Sbjct: 1070 GPPGKTGPVGPQGPSGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPM--------------VREKIMFLLFFLSGHPGLIGLIGPPGEQGE---KGDRGLPGPQGAAGSKGDNGP---QGPKGSKGSTGPAGQKGDTGTV--------------------------------GPPGPPGPPGEVIQPLPIHSPKKSRRSIDMQADEAGTMLDYGEGMEDIFSSLNNLKQDIERMKYPMG-TQNNPARSCKDLQLAHPEFPDGEYYIDPNQGCSGDSFKVYCNFTAGGETCIFPDKKSNGVRISSWPKEVPGSWFSEFK-RGKILSYVDAEGNSINMVQMTFLKLLTASARQNLTYSCHQSVAWHDATTDSYDR-------ALHFLGSND---------EEISYDNNPY----------IKALSDGCAVRKGYGKTVLEINTPKIDQVPIVDVKFTDFGDPN-QKFGFEVGPVCF 1463          

HSP 2 Score: 105.145 bits (261), Expect = 5.070e-22
Identity = 64/218 (29.36%), Postives = 98/218 (44.95%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRRK----AFRYESGNSLMAKSDVLF-QGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
            VD+L  ++      G      FC N R      A++      L A +  LF  G FP++FSIL T K +A     + SI N+     L V  GR     Y D    P+   +  L    +L DG+WHR+  SV+K +V++++DC+   +  L R+      + G          + SF G +  LL+V     A+  C+ Y+PDC+    D +  Q P
Sbjct:   29 VDVLKVLEFPTVPEGVEKTSGFCTNRRASQPDAAYKISKNAQLSAPTKQLFPDGVFPEDFSILTTIKPKAGIQSFLLSIYNEKGVQQLGVEVGRSPVFLYEDQEGKPAPEDY-PLFRALNLADGKWHRVGISVEKKTVTIVVDCKKKLTKPLGRSDHASIDTKGITVFGTRILDEESFEGDIQQLLIVADPKAAYDYCEHYSPDCDVPHKDTLQAQEP 245          

HSP 3 Score: 61.6178 bits (148), Expect = 9.195e-9
Identity = 47/108 (43.52%), Postives = 63/108 (58.33%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQV------GTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            Y G RG++G+ G +G  G  G+       G +G++G+KG +GE G+ GPRG +G  G KG  G  GE+GP+G  G KG  G  G PGY G  G KG  G  G +G+ G
Sbjct:  677 YPGPRGVKGADGVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGVPGLPGYPGRQGPKGVAGKAGPRGQRG 784          

HSP 4 Score: 58.9214 bits (141), Expect = 6.783e-8
Identity = 109/299 (36.45%), Postives = 125/299 (41.81%), Query Frame = 2
Query: 3170 KGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGE---------------------LGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG 3994
            KG  GD G  GP    G PG+IG+ G  G+ GA GA G PG  G+KGD G  G  G  G  G  G  G  G  G  G+ G +G  G  GP+G+ GE                        QG PG +G   PIG PGP G  G  G  G  G  G  G+ G       PG  G  GL+G  G  G  G  GE G  GP G AG  G+ GP G                              S G  GK+G PG  G    +G KG  G  G  G +G  GP G  G +G  G PGPKG
Sbjct:  546 KGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKGHRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESYLGHRFSCALHHKHLIIFHCCHFQGQPGPQG---PIGYPGPRGVKGADGVRGLKGGKGEKGEDGF------PGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQG------------------------------SAGEKGKLGVPGLPGYPGRQGPKGVAGKAGPRGQRGPTGPRGARGARGPTGKPGPKG 805          

HSP 5 Score: 55.0694 bits (131), Expect = 9.337e-7
Identity = 116/293 (39.59%), Postives = 133/293 (45.39%), Query Frame = 2
Query: 2882 KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPG------RIGEAGPPGKT---GASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNG 3733
            KG  G  GP G +G  GP G+ G  G RG  G  G+ G  G  GS+G +G  G PG PG+ G                    G  G  G PGP GE G +G+ G IGP G  G      R   A              +GQP   G +G  G  GPRG +G D   G  G KG KG +G+ G  G  G+ G +G  GELG+ GP G  G  GP G  GP G SGP G  G  G  G             PGLPG  G QGP    G  G+AG RG RGP G  G  G RGP G
Sbjct:  546 KGESGDHGPQGPRGIQGPPGQIGKPGKRGRAGADGARGMPGESGSKGDRGFDGLPGLPGEKG------------------HRGETGPAGPPGPTGEDGPRGEDGEIGPRGLAGESYLGHRFSCALHHKHLIIFHCCHFQGQP---GPQGPIGYPGPRGVKGAD---GVRGLKGGKGEKGEDGFPGFKGDMGLKGDRGELGLAGPRGEDGPEGPKGRAGPNGESGPQGSAGEKGKLGV------------PGLPGYPGRQGP---KGVAGKAGPRGQRGPTGPRGARGARGPTG 799          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Xenopus
Match: ENSXETT00000017769.1 (collagen, type XXVII, alpha 1 [Source:NCBI gene;Acc:100490770])

HSP 1 Score: 156.762 bits (395), Expect = 1.593e-37
Identity = 286/898 (31.85%), Postives = 378/898 (42.09%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTG------------LKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASG-------------------------AEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEG------ELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM------GPIG---------PVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRT-PGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            PG  GPSG PG+ GI G  G  G +G +G  G+ G+ GPQG   A+G+ GP GE G KGD G  G  G  G  G+RGDPG +G                                    E G+ G  G KG +G  GP+GEKG+ G  G  G            ++G  G+ G +G +GK G  GS+G +G  GE G PGD G  G                               +G KG  G  GP G+PGR+G  G PG  G  G                         AEG PG  G +G  G  G RGE G     G  G  G +G RG  G+QG  G+SGP+G++G       +G++G PG+ GE G  G PG +G +GP G  G  G  G +G  G  G  GSPGL G+ G  G  G +GT G+ G  G  G PG  G  G RG  G  G +G  G                      RG++G +G  G KGE   KG  G PG +G  G  G  GP G  G  G +G  GPKG  GLM      GP+G         PVG+ G  G  G  GP GPPGP G  G PG S       +       +       SSG   +ST     VD    +F  +  +++ I+ I+ P G  +D+ AR C D+   +++  DG Y+IDPN G   D+IEV C F+E  +TC+  + +                     ++E  + R            QMNFL   +S++ Q I F C     +  S  S+  +FK  +    H F +            EG              +F   +  DGC  +      +     T D +  PI+D       + G+ T+ L V S+CF
Sbjct:  959 PGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQG---AKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEG------------------------------------ESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTG------------------------------NQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPG---DPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPG---------------------KRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYRQEDFAASYQALM---DSSGTSYQSTNYM-MVDNGSEIFKTLHYLSSLIQSIKSPLG-TRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKS--------------------KMEFEVGR-----------VQMNFLHLLSSEAMQSITFHCLNVPAWEKS--SKAVRFKAWS---GHIFGT------------EG--------------MFQPEVSVDGCKVQDGRWHQTVLIFRTQDPTQFPIVDVLNLPLPSPGM-TIHLEVGSVCF 1695          

HSP 2 Score: 108.997 bits (271), Expect = 6.369e-23
Identity = 176/529 (33.27%), Postives = 207/529 (39.13%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG------------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GP G  G +G  G  GI GK+G IGP+G +G RG    PGP G  G  G SGP GE G  G+ G  G QGP G  GE                                              G +G  G KG  GP G  G KGD+GPLG  G +G  G+ G  G EG++GL G  G +G  G+PG  G+ G                                 EKGV+G  G            + G  G PG+ GE G PG+ G+ G EG  G  G  GD G  G                                 N GP+G  G  G  G PGR G  G  G+PG           G  GPPG  G  G  G  G  G  GKA  +G  G  G +G  G+ GDRG PG  G  GQ GP GP                              RG  G+ G PG  G R L+G++GS GT+G  G  G+PG  G  G  G  G  GP+G  G  G  GP G DG
Sbjct:  918 GPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGI---PGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGE---------------------------------------------IGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKG---------------------------------EKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTG---------------------------------NQGPKGSRGTRGPVGSPGRMGAQGEPGMPG---------YQGHKGPPGPLGPPGIKGEKGEQGDDGKA--EGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGP------------------------------RGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDG 1291          

HSP 3 Score: 106.686 bits (265), Expect = 3.009e-22
Identity = 211/541 (39.00%), Postives = 255/541 (47.13%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPR---------------GQSGNPGPQGIRGARGQSGPTG---ELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ------GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV------GSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            GPPG  GP G+ GKPG  G  G  G  G  G                 G+ G PG  G  G +G++G TG   ++G +G +G  GP G TG  GE+GD G  G PGP G  G +G  G+PG  G  G  G PG  G  G  GVRG  GR+G+ GP GP GE G  G  G +G++G +G +G +G  G+ G+ G  G  G+SG  G PG+ G+                                  G  G  G  GP GA G IG  GP    GA G  G PG  G KGD G LG  GEQGL                         G  G  G+EGE G+ GP G KGE   IG PGP G  G  G +G  G  G  G +G  G  G  G PGK G +G  G  G++      GE G  GD+GP G  G  G RG  GP+G  G  G +G+PG  G  G  G PGP G  G+ G+ GE G  G+   +G  G+PG  G V      G  G+PG PG  GP+G RGN G +G  G  GP G
Sbjct:  762 GPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGIT---------------------------------GNPGPAGAQGPVGALGEIGHKGP---QGAKGEPGPPGETGLKGDLGPLGIPGEQGL------------------------IGQRGDPGLEGESGLTGPDGMKGE---IGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGK--AEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRG 1237          

HSP 4 Score: 89.3521 bits (220), Expect = 5.442e-17
Identity = 156/514 (30.35%), Postives = 200/514 (38.91%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPT------------GDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTG------LMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKR 3973
             G PG +G  G PG+ G  G EG +G  G  G +G +GN GP+G RG RG  G  G +G++G+ G  G QG              G+KGE+GD G                                        +G  G  G +G VG  G  GE GD G  G+ G +G RG  G +G  G +G      L GS+G  G+ G+PG PG++G +                                 G  G IG+ GP G  GR G+ GP G  G  G +G PG+KG +GD G +G  G  G                             G  GV G  G QGPPG +GE G   LPG +G  G                          G+ G  GL G  G  GT+G+ G+ G+ G PGIAG  G +GP G +G                            +G+ G       KG   L G +G+ G +G VG  G PGP G  G QG  
Sbjct: 1088 EGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKA--------------------------------------EGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAH---------------------------------GQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLG---------------------------KRGNTGVPGLPGAQGPPGFRGERG---LPGEIGLPGKR------------------------GMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGF---------------------------KGIQGP------KGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDN 1443          

HSP 5 Score: 85.1149 bits (209), Expect = 1.086e-15
Identity = 202/540 (37.41%), Postives = 234/540 (43.33%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGD---LGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGD---IGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPG------------PVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER---YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RGP G+ G  G PG+ G+ G  G  GPKG+   +GP G  G PG  G RG  G  G  G +G  G  G FG +GP G  G                                   G  G  GP G  G  G +G  G+VG  G  G KG  G  G++GLKG +G  G  G  G  G  G +G QG+ G PG  G  GV G+ G++G  G  GP GP G  G  G PG  G +G+KG     G  GP G PG  G+ GP G  GA G EG PG KG  G  G  GP+GE G                           N+G  G  GE G+QG  G  GE G  G  G              GT G  G  G PG PG  G  G  G  GSPG+ G  G +G  G  G  GE G  G +GP GI G  G  GP G +G                            RG+ G  G PG  G       KGI G+PG  GA G  G  G  G  GPQG +G PGP GETGL G +GP+G  GE
Sbjct:  572 RGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGN---------------------------------PGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAG---------------------------NTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMG---------------------------ERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGE 1024          

HSP 6 Score: 68.5514 bits (166), Expect = 1.134e-10
Identity = 199/542 (36.72%), Postives = 236/542 (43.54%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG---PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGV------------KGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP------------GPPGLTGPQGK---RGNPGPKGETGLMGPIGPVGEDG 4039
             GP G  G PG+   +   G KGD G  G +G PGP   +G +G  GP G  G  G R   GP+G                                                     G +G  GR+G  GPIG   P G +G +GP G  GL G  GE G+ G  GK G MG  G  G  GE G PG  G                       G  G PGP G  G  GD+G +G  G PG  G  G PG +G SG +G  G+            +G KG  G+ GP G +G  G +G  G KG  G  G PG +G  G+ GP G  G  GI+G PG +G  GP G PG  G  GP G  GAPG  G  G+          G PG  G +GP G +G  G +G+ G++G  G  G +G+ G  G  G  G  G  G PG  G MG  GNPGP GS G  G  G PG +G   ++G KG  G  G  G  GEP            G  GL GPQGK   RG PGP G  G+ GP GP GE G
Sbjct:  515 HGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVR---GPEG---------------------------------------------------SPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDG---------------------NPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRK---------GYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKG 972          

HSP 7 Score: 65.0846 bits (157), Expect = 1.433e-9
Identity = 196/582 (33.68%), Postives = 241/582 (41.41%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARG------------------------------QSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXX------XXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---------------------------PGPKGETGLMGPIGPVGEDGE 4042
            +G PG  G SGL G  GI G  G  G  G  G +G  G PGP G+RG  G                              ++GP G  G  G RG  GP+G  G +G+ G  G  G                                      G+ G  GRKG  G +G +G KG+ G  G++G  G +G  G  G  G+TG+ G +G +G  G PG PG+ G  G  G  G  GS GP GP G  G  G PG RG KG +G  G  GP G PG  G  GP   +G  G +G  G +G  G+ GI GP G  G+                           SGP G  GE GI G PG  G  GP+G  G +G                    G  G  G PG PG+ GL+G  G  G  GE G  G RG PG+ G  G  GP+G  G +G        G +G  G  G  GP G  G  G RG++GK G+ G+KG+   KG KG  G +G  G  G+ GP G  GP+G RG                            PGP G  G+ G  G  G+DG+
Sbjct:  686 KGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPG------------------------------------APGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGP---SGIQGKQGLIGPQGKMGERGIPGPNGFPGI---------------------------SGPSGPPGEKGITGNPGPAGAQGPVGALGEIG------------------HKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGK 1183          

HSP 8 Score: 60.4622 bits (145), Expect = 3.318e-8
Identity = 109/260 (41.92%), Postives = 142/260 (54.62%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTG 3256
            RGP G  G  G PG PG  G++G  GP+G+ G +G  G+ GP   QG+RG++G  G  G+ G  G+ G  G  G  G  G RG  G QG  GPAGP+G  G  GSPG KG +G  G         E G+ G LG++G+ G  G  G +G  G  G+ GL G  G  G +G EG  GL G++G  G  G+PG PG+ G  G  G  G +G  G  G  G+QG  G  G  G++G  G +G++GP G PG +G+ G  G  G
Sbjct: 1194 RGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDG---------EIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNG 1444          

HSP 9 Score: 58.151 bits (139), Expect = 1.486e-7
Identity = 43/101 (42.57%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KG  G++G  G  G+ G  GQ G  G  G +GV G  G +GP GP+GI G  G  G++GE   +G DG  G  G++G  G  G PG+ G++GP G +G  G
Sbjct: 1245 KGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGE---QGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERG 1342          

HSP 10 Score: 58.151 bits (139), Expect = 1.715e-7
Identity = 176/566 (31.10%), Postives = 219/566 (38.69%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPG---------KPGIDGKEGSIGPK---------GDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTG---DKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEA---------------------------------------------GPPGKTGASGAEG---QPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN 3979
            G PG RG  G PG          PG+DG  G +G           GD+G  G  G PG +G++G  G SG +G  G KG +G  G  G  G   DKG +G PG  G  G  G  G  G  G  G  G  G  G PG  GP GE G  G+ G+ G+ G  GP G+ G  G  G  G  G  G  G  G +G  G++G+ GP+G +G  G  G  G                                 E+G++G IG +G TG  G  G+                                              GP G+ GA G +G   +PG+ GTKG +GI   +G+QGL                            GPQG  GE GI GP G  G  GP G PG  G +                           G PG AG QGP G  G  G  G +G +G PG  G  G +G  GP+GI                         G +GL G+ G+PG +GE  L G  G  G IG  G +GE G  G+ G +GK G 
Sbjct:  624 GVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIG---------------------------------EQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGI---QGKQGL---------------------------IGPQGKMGERGIPGPNGFPGISGPSGPPGEKGIT---------------------------GNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGI------------------------PGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGP 1075          

HSP 11 Score: 56.225 bits (134), Expect = 6.437e-7
Identity = 44/101 (43.56%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            K   G++G  G  G  G  G +  +G+ G  G +G  G  G RGPEG  G KG  G QG  GP G  G KG+ G +GPPG  G PG  G RG  G  G+ G
Sbjct:  530 KAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRG 630          

HSP 12 Score: 55.4546 bits (132), Expect = 1.067e-6
Identity = 46/106 (43.40%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP---PGYAGNPGIKGSRGPVGNQGRDGE 2134
            Y G  G  G  G  G  G  G  G RG  G++G KG  G++G  G  G +G  G  G  G TGPRG  G +G EG  GP   PG  G+PG+K      G QG DGE
Sbjct: 1211 YPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLK------GEQGDDGE 1310          

HSP 13 Score: 55.0694 bits (131), Expect = 1.572e-6
Identity = 43/100 (43.00%), Postives = 54/100 (54.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G +G  G  G +G  GP G++G RG  G  G  G  G  GP G +GI G  G  G QG  G  G+ G+KG +G+ G PG  G  G+KG  GP+G  G  G
Sbjct:  927 GTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQG 1026          

HSP 14 Score: 54.6842 bits (130), Expect = 1.957e-6
Identity = 37/91 (40.66%), Postives = 55/91 (60.44%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            G  G +G  GAKG+ GP G+ G +G++G  G+ GE         +G+ G +GD G++GE+G  G DG KG+ G  GP G  G  G++G++G
Sbjct:  990 GEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGE---------QGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKG 1071          

HSP 15 Score: 54.6842 bits (130), Expect = 2.129e-6
Identity = 41/101 (40.59%), Postives = 54/101 (53.47%), Query Frame = 2
Query: 1835 ARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
             RG +G  GA+G  GP+G+ G  G  G  G++G+ G+ GP+G  G  G+ G  G  G +GP G  G K         G  GNPG  G++GPVG  G  G K
Sbjct:  904 VRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEK---------GITGNPGPAGAQGPVGALGEIGHK 995          

HSP 16 Score: 52.7582 bits (125), Expect = 7.531e-6
Identity = 44/100 (44.00%), Postives = 53/100 (53.00%), Query Frame = 2
Query: 1847 RGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPR---GPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
             G+ GA GD GP G  G RGE G  G  G+ G +GPR   G +G+ G  G  G+QG  G +G  G KG  G  G  G  G PG  G  GP G  GR G++
Sbjct: 1185 EGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQE 1284          

HSP 17 Score: 52.373 bits (124), Expect = 9.375e-6
Identity = 45/98 (45.92%), Postives = 54/98 (55.10%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            +G  G  G SGAKG+ G  GQ G  GE+G  G+ G +   GP+GP G  G KG  G +G  G  GK+G  G  G VGP    GNPG  G RG  G+ G
Sbjct: 1353 RGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLF---GPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGP---NGNPGPVGDRGSQGDNG 1444          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Xenopus
Match: ENSXETT00000032329.1 (collagen, type XXVII, alpha 1 [Source:NCBI gene;Acc:100490770])

HSP 1 Score: 156.762 bits (395), Expect = 1.606e-37
Identity = 286/898 (31.85%), Postives = 378/898 (42.09%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTG------------LKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASG-------------------------AEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEG------ELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM------GPIG---------PVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRT-PGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            PG  GPSG PG+ GI G  G  G +G +G  G+ G+ GPQG   A+G+ GP GE G KGD G  G  G  G  G+RGDPG +G                                    E G+ G  G KG +G  GP+GEKG+ G  G  G            ++G  G+ G +G +GK G  GS+G +G  GE G PGD G  G                               +G KG  G  GP G+PGR+G  G PG  G  G                         AEG PG  G +G  G  G RGE G     G  G  G +G RG  G+QG  G+SGP+G++G       +G++G PG+ GE G  G PG +G +GP G  G  G  G +G  G  G  GSPGL G+ G  G  G +GT G+ G  G  G PG  G  G RG  G  G +G  G                      RG++G +G  G KGE   KG  G PG +G  G  G  GP G  G  G +G  GPKG  GLM      GP+G         PVG+ G  G  G  GP GPPGP G  G PG S       +       +       SSG   +ST     VD    +F  +  +++ I+ I+ P G  +D+ AR C D+   +++  DG Y+IDPN G   D+IEV C F+E  +TC+  + +                     ++E  + R            QMNFL   +S++ Q I F C     +  S  S+  +FK  +    H F +            EG              +F   +  DGC  +      +     T D +  PI+D       + G+ T+ L V S+CF
Sbjct:  959 PGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQG---AKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEG------------------------------------ESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTG------------------------------NQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKAEGTPGAPGDRGPVGDGGDRGEPG---DPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPG---------------------KRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNGMPGPRGPPGPRGKPGPPGPSVILDSYRQEDFAASYQALM---DSSGTSYQSTNYM-MVDNGSEIFKTLHYLSSLIQSIKSPLG-TRDNPARICRDLMNCQQKMGDGTYWIDPNIGCSSDTIEVTCNFTEGGQTCLKPITKS--------------------KMEFEVGR-----------VQMNFLHLLSSEAMQSITFHCLNVPAWEKS--SKAVRFKAWS---GHIFGT------------EG--------------MFQPEVSVDGCKVQDGRWHQTVLIFRTQDPTQFPIVDVLNLPLPSPGM-TIHLEVGSVCF 1695          

HSP 2 Score: 108.612 bits (270), Expect = 6.643e-23
Identity = 176/529 (33.27%), Postives = 207/529 (39.13%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG------------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GP G  G +G  G  GI GK+G IGP+G +G RG    PGP G  G  G SGP GE G  G+ G  G QGP G  GE                                              G +G  G KG  GP G  G KGD+GPLG  G +G  G+ G  G EG++GL G  G +G  G+PG  G+ G                                 EKGV+G  G            + G  G PG+ GE G PG+ G+ G EG  G  G  GD G  G                                 N GP+G  G  G  G PGR G  G  G+PG           G  GPPG  G  G  G  G  G  GKA  +G  G  G +G  G+ GDRG PG  G  GQ GP GP                              RG  G+ G PG  G R L+G++GS GT+G  G  G+PG  G  G  G  G  GP+G  G  G  GP G DG
Sbjct:  918 GPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGI---PGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGE---------------------------------------------IGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKG---------------------------------EKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTG---------------------------------NQGPKGSRGTRGPVGSPGRMGAQGEPGMPG---------YQGHKGPPGPLGPPGIKGEKGEQGDDGKA--EGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGP------------------------------RGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDG 1291          

HSP 3 Score: 106.301 bits (264), Expect = 3.415e-22
Identity = 211/541 (39.00%), Postives = 255/541 (47.13%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPR---------------GQSGNPGPQGIRGARGQSGPTG---ELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ------GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV------GSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            GPPG  GP G+ GKPG  G  G  G  G  G                 G+ G PG  G  G +G++G TG   ++G +G +G  GP G TG  GE+GD G  G PGP G  G +G  G+PG  G  G  G PG  G  G  GVRG  GR+G+ GP GP GE G  G  G +G++G +G +G +G  G+ G+ G  G  G+SG  G PG+ G+                                  G  G  G  GP GA G IG  GP    GA G  G PG  G KGD G LG  GEQGL                         G  G  G+EGE G+ GP G KGE   IG PGP G  G  G +G  G  G  G +G  G  G  G PGK G +G  G  G++      GE G  GD+GP G  G  G RG  GP+G  G  G +G+PG  G  G  G PGP G  G+ G+ GE G  G+   +G  G+PG  G V      G  G+PG PG  GP+G RGN G +G  G  GP G
Sbjct:  762 GPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGIT---------------------------------GNPGPAGAQGPVGALGEIGHKGP---QGAKGEPGPPGETGLKGDLGPLGIPGEQGL------------------------IGQRGDPGLEGESGLTGPDGMKGE---IGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGK--AEGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRG 1237          

HSP 4 Score: 89.3521 bits (220), Expect = 5.971e-17
Identity = 156/514 (30.35%), Postives = 200/514 (38.91%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPT------------GDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTG------LMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKR 3973
             G PG +G  G PG+ G  G EG +G  G  G +G +GN GP+G RG RG  G  G +G++G+ G  G QG              G+KGE+GD G                                        +G  G  G +G VG  G  GE GD G  G+ G +G RG  G +G  G +G      L GS+G  G+ G+PG PG++G +                                 G  G IG+ GP G  GR G+ GP G  G  G +G PG+KG +GD G +G  G  G                             G  GV G  G QGPPG +GE G   LPG +G  G                          G+ G  GL G  G  GT+G+ G+ G+ G PGIAG  G +GP G +G                            +G+ G       KG   L G +G+ G +G VG  G PGP G  G QG  
Sbjct: 1088 EGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGKA--------------------------------------EGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAH---------------------------------GQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDGEIGLAGTLG---------------------------KRGNTGVPGLPGAQGPPGFRGERG---LPGEIGLPGKR------------------------GMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGF---------------------------KGIQGP------KGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDN 1443          

HSP 5 Score: 85.1149 bits (209), Expect = 1.059e-15
Identity = 202/540 (37.41%), Postives = 234/540 (43.33%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGD---LGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGD---IGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPG------------PVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER---YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RGP G+ G  G PG+ G+ G  G  GPKG+   +GP G  G PG  G RG  G  G  G +G  G  G FG +GP G  G                                   G  G  GP G  G  G +G  G+VG  G  G KG  G  G++GLKG +G  G  G  G  G  G +G QG+ G PG  G  GV G+ G++G  G  GP GP G  G  G PG  G +G+KG     G  GP G PG  G+ GP G  GA G EG PG KG  G  G  GP+GE G                           N+G  G  GE G+QG  G  GE G  G  G              GT G  G  G PG PG  G  G  G  GSPG+ G  G +G  G  G  GE G  G +GP GI G  G  GP G +G                            RG+ G  G PG  G       KGI G+PG  GA G  G  G  G  GPQG +G PGP GETGL G +GP+G  GE
Sbjct:  572 RGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDGN---------------------------------PGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAG---------------------------NTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMG---------------------------ERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGE 1024          

HSP 6 Score: 68.5514 bits (166), Expect = 1.213e-10
Identity = 199/542 (36.72%), Postives = 236/542 (43.54%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG---PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGV------------KGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP------------GPPGLTGPQGK---RGNPGPKGETGLMGPIGPVGEDG 4039
             GP G  G PG+   +   G KGD G  G +G PGP   +G +G  GP G  G  G R   GP+G                                                     G +G  GR+G  GPIG   P G +G +GP G  GL G  GE G+ G  GK G MG  G  G  GE G PG  G                       G  G PGP G  G  GD+G +G  G PG  G  G PG +G SG +G  G+            +G KG  G+ GP G +G  G +G  G KG  G  G PG +G  G+ GP G  G  GI+G PG +G  GP G PG  G  GP G  GAPG  G  G+          G PG  G +GP G +G  G +G+ G++G  G  G +G+ G  G  G  G  G  G PG  G MG  GNPGP GS G  G  G PG +G   ++G KG  G  G  G  GEP            G  GL GPQGK   RG PGP G  G+ GP GP GE G
Sbjct:  515 HGPKGQKGDPGLSPGKAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVR---GPEG---------------------------------------------------SPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRGTMGRPGFPGDFGERGPPGLDG---------------------NPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRK---------GYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKG 972          

HSP 7 Score: 64.6994 bits (156), Expect = 1.626e-9
Identity = 196/582 (33.68%), Postives = 241/582 (41.41%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARG------------------------------QSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXX------XXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---------------------------PGPKGETGLMGPIGPVGEDGE 4042
            +G PG  G SGL G  GI G  G  G  G  G +G  G PGP G+RG  G                              ++GP G  G  G RG  GP+G  G +G+ G  G  G                                      G+ G  GRKG  G +G +G KG+ G  G++G  G +G  G  G  G+TG+ G +G +G  G PG PG+ G  G  G  G  GS GP GP G  G  G PG RG KG +G  G  GP G PG  G  GP   +G  G +G  G +G  G+ GI GP G  G+                           SGP G  GE GI G PG  G  GP+G  G +G                    G  G  G PG PG+ GL+G  G  G  GE G  G RG PG+ G  G  GP+G  G +G        G +G  G  G  GP G  G  G RG++GK G+ G+KG+   KG KG  G +G  G  G+ GP G  GP+G RG                            PGP G  G+ G  G  G+DG+
Sbjct:  686 KGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPG------------------------------------APGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIGEQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGP---SGIQGKQGLIGPQGKMGERGIPGPNGFPGI---------------------------SGPSGPPGEKGITGNPGPAGAQGPVGALGEIG------------------HKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGPPGPSGIPGVRGVEGKPGKQGEKGKPGQKGSKGFEGHLGETGVPGDQGPTGNQGPKGSRGTRGPVGSPGRMGAQGEPGMPGYQGHKGPPGPLGPPGIKGEKGEQGDDGK 1183          

HSP 8 Score: 60.4622 bits (145), Expect = 3.610e-8
Identity = 109/260 (41.92%), Postives = 142/260 (54.62%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTG 3256
            RGP G  G  G PG PG  G++G  GP+G+ G +G  G+ GP   QG+RG++G  G  G+ G  G+ G  G  G  G  G RG  G QG  GPAGP+G  G  GSPG KG +G  G         E G+ G LG++G+ G  G  G +G  G  G+ GL G  G  G +G EG  GL G++G  G  G+PG PG+ G  G  G  G +G  G  G  G+QG  G  G  G++G  G +G++GP G PG +G+ G  G  G
Sbjct: 1194 RGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGEQGDDG---------EIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERGLPGEIGLPGKRGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLFGPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGPNGNPGPVGDRGSQGDNG 1444          

HSP 9 Score: 58.5362 bits (140), Expect = 1.449e-7
Identity = 43/101 (42.57%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KG  G++G  G  G+ G  GQ G  G  G +GV G  G +GP GP+GI G  G  G++GE   +G DG  G  G++G  G  G PG+ G++GP G +G  G
Sbjct: 1245 KGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLKGE---QGDDGEIGLAGTLGKRGNTGVPGLPGAQGPPGFRGERG 1342          

HSP 10 Score: 57.7658 bits (138), Expect = 1.979e-7
Identity = 176/566 (31.10%), Postives = 219/566 (38.69%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPG---------KPGIDGKEGSIGPK---------GDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTG---DKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEA---------------------------------------------GPPGKTGASGAEG---QPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN 3979
            G PG RG  G PG          PG+DG  G +G           GD+G  G  G PG +G++G  G SG +G  G KG +G  G  G  G   DKG +G PG  G  G  G  G  G  G  G  G  G  G PG  GP GE G  G+ G+ G+ G  GP G+ G  G  G  G  G  G  G  G +G  G++G+ GP+G +G  G  G  G                                 E+G++G IG +G TG  G  G+                                              GP G+ GA G +G   +PG+ GTKG +GI   +G+QGL                            GPQG  GE GI GP G  G  GP G PG  G +                           G PG AG QGP G  G  G  G +G +G PG  G  G +G  GP+GI                         G +GL G+ G+PG +GE  L G  G  G IG  G +GE G  G+ G +GK G 
Sbjct:  624 GVPGKRGTMGRPGFPGDFGERGPPGLDGNPGELGAPGPPGVPGFIGDMGAVGAVGFPGLKGLKGLPGNSGESGLKGDKGIQGFPGIPGDIGFQGDKGSQGLPGPPGMRGKTGVMGKPGDKGFAGFPGPPGPEGFPGDIGPPGENGPEGIKGKPGARGFPGPRGQPGHEGDEGPIGPPGAPGLEGFPGRKGYPGILGAEGPKGEAGNTGRSGKIG---------------------------------EQGLQGFIGPVGETGIAGEKGDRGGIGSPGPPGEKGTMGHPGNPGPPGSPGPPGPPGSPGSRGSPGVRGPKGRRGARGPDGPAGEPGLAGTKGPSGI---QGKQGL---------------------------IGPQGKMGERGIPGPNGFPGISGPSGPPGEKGIT---------------------------GNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGI------------------------PGEQGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKGKDGP 1075          

HSP 11 Score: 56.225 bits (134), Expect = 5.917e-7
Identity = 44/101 (43.56%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            K   G++G  G  G  G  G +  +G+ G  G +G  G  G RGPEG  G KG  G QG  GP G  G KG+ G +GPPG  G PG  G RG  G  G+ G
Sbjct:  530 KAINGLKGDPGLPGLTGVPGPLSRKGQKGYPGPQGHPGEPGVRGPEGSPGAKGYPGRQGLPGPIGVSGPKGNRGYIGPPGLFGLPGADGERGLPGVPGKRG 630          

HSP 12 Score: 55.4546 bits (132), Expect = 1.040e-6
Identity = 46/106 (43.40%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP---PGYAGNPGIKGSRGPVGNQGRDGE 2134
            Y G  G  G  G  G  G  G  G RG  G++G KG  G++G  G  G +G  G  G  G TGPRG  G +G EG  GP   PG  G+PG+K      G QG DGE
Sbjct: 1211 YPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQEGPAGPDGIPGKDGSPGLK------GEQGDDGE 1310          

HSP 13 Score: 55.0694 bits (131), Expect = 1.470e-6
Identity = 43/100 (43.00%), Postives = 54/100 (54.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G +G  G  G +G  GP G++G RG  G  G  G  G  GP G +GI G  G  G QG  G  G+ G+KG +G+ G PG  G  G+KG  GP+G  G  G
Sbjct:  927 GTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEKGITGNPGPAGAQGPVGALGEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGEQG 1026          

HSP 14 Score: 54.6842 bits (130), Expect = 1.845e-6
Identity = 37/91 (40.66%), Postives = 55/91 (60.44%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            G  G +G  GAKG+ GP G+ G +G++G  G+ GE         +G+ G +GD G++GE+G  G DG KG+ G  GP G  G  G++G++G
Sbjct:  990 GEIGHKGPQGAKGEPGPPGETGLKGDLGPLGIPGE---------QGLIGQRGDPGLEGESGLTGPDGMKGEIGQPGPEGEKGEKGVEGTKG 1071          

HSP 15 Score: 54.6842 bits (130), Expect = 1.974e-6
Identity = 41/101 (40.59%), Postives = 54/101 (53.47%), Query Frame = 2
Query: 1835 ARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
             RG +G  GA+G  GP+G+ G  G  G  G++G+ G+ GP+G  G  G+ G  G  G +GP G  G K         G  GNPG  G++GPVG  G  G K
Sbjct:  904 VRGPKGRRGARGPDGPAGEPGLAGTKGPSGIQGKQGLIGPQGKMGERGIPGPNGFPGISGPSGPPGEK---------GITGNPGPAGAQGPVGALGEIGHK 995          

HSP 16 Score: 52.7582 bits (125), Expect = 7.282e-6
Identity = 44/100 (44.00%), Postives = 53/100 (53.00%), Query Frame = 2
Query: 1847 RGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPR---GPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
             G+ GA GD GP G  G RGE G  G  G+ G +GPR   G +G+ G  G  G+QG  G +G  G KG  G  G  G  G PG  G  GP G  GR G++
Sbjct: 1185 EGTPGAPGDRGPVGDGGDRGEPGDPGYPGQDGPEGPRGNAGQQGLPGHSGPRGLQGLRGSKGTVGLKGKPGKPGESGAHGQPGAIGVTGPRGVVGRQGQE 1284          

HSP 17 Score: 52.373 bits (124), Expect = 8.838e-6
Identity = 45/98 (45.92%), Postives = 54/98 (55.10%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            +G  G  G SGAKG+ G  GQ G  GE+G  G+ G +   GP+GP G  G KG  G +G  G  GK+G  G  G VGP    GNPG  G RG  G+ G
Sbjct: 1353 RGMEGGMGLSGAKGELGTKGQPGDPGEMGFPGIAGLF---GPKGPPGDVGFKGIQGPKGPLGLMGKEGAGGPMGIVGP---NGNPGPVGDRGSQGDNG 1444          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Xenopus
Match: COL5A1 (collagen type V alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:2209])

HSP 1 Score: 147.902 bits (372), Expect = 6.949e-35
Identity = 271/924 (29.33%), Postives = 371/924 (40.15%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARG---QSGPTGELG---------SKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPV----------------GTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQG---------------EAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGA------VGSKGEPG------PP----------------GLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDA------------LFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            GP G+ GPSGL G+ G  G +G  GP+G  GP G+ G  G  G  GARG   Q+GP G+ G          KG RG  GPQGP G  G RG                                               G+    G +GP GP GE G  GP G+ G  G  G +G  G +GK G         V G PG PG  G                        +        E  V G  G  GP G   + G  GP G+ G  GA G+PG KG  G+TG +G RG        GP G  G +G  G  G++GS G+ GP G+ G+    GPPG +G  G  GLPGP+                G  G +G  G  G PG  G  G  GP G  G PG+ G QGPAG  G QG               E G++G+ G PG  GN G +G           GP G PGP GP+G  G  G  G  G  G+ G  GQKG+   +G  G PG +G       + +  +P       PP                GL GP G++G    KG+ GL GP GP G  GE                  +    +                 I+  P  SS R +RS  A   VD  +A            +F  ++ +  +I++++ P G N+ + ARTC D+ L   +  DG+Y+IDPN+G  +DS +V C F+   ++CI   K+  G      P +   +W +  +  G +    D +   +   QM F++  ++   Q I + C QSV + D+     N +  A   +                   G  D+ +  D    P     +  DGC  +     +  +L+T+ +   PI+D   ND+ +  +   G  V  +CF
Sbjct:  524 GPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSV-----------------------------------------GIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLG---------VPGLPGYPGRQG--------------------PKVTLSSSLIKPPENSVPGTPGKPGPRG---QRGPTGPRGERGPRGATGKPGPKGPTGETGPMGERGHP------GPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGK---DGPPGLRGFTGERGLPGPICFSSLLKTLFSFQGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKG---------EQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQVMPTNIANVSKPTYIYISTPPLCCHRGHVLLELSCLIGLIGPPGEQGE---KGDRGLPGPQGPHGAKGEQVCIFSSHKETSLNDGEHQSPNFEGPPGEV-----------IQPLPIHSSKRTRRSIDASQVVDDGNADNYMDYADGMEEIFGSLNSLKQEIEQMKHPLG-NQQNPARTCKDLLLCHPELPDGEYWIDPNQGCSRDSFKVFCNFTAGGESCIFPDKKSEGARITSWPKENPGSWFSEFK-RGKLLSYVDSNGNPIGVVQMTFMKLLSASVKQNITYNCYQSVAWHDATT---NSYDKAIRFL-------------------GSNDEEMSYDN--NPSIRAIM--DGCASKKGYQKTVLELDTSKVEQAPIVDIMFNDFGDP-VQKFGFEVGPVCF 1313          

HSP 2 Score: 107.457 bits (267), Expect = 1.419e-22
Identity = 62/222 (27.93%), Postives = 100/222 (45.05%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLFQG-KFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTS-------FMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
             DLL A++  +   G      FC   +       AFR      L A ++ LF    FP++FS+L T K +      + SI N+   + + +T G +    Y D            F N+    +L+DG+WHR+AFSV K +++L +DC    ++ L R+  P+  + G +        +  F G +  L++V  A  A   C+ Y PDC+    D   +Q P
Sbjct:   36 ADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNI----NLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDP 253          

HSP 3 Score: 77.7962 bits (190), Expect = 1.589e-13
Identity = 181/559 (32.38%), Postives = 222/559 (39.71%), Query Frame = 2
Query: 2498 GTRGPSGLPGKP---------GIDGKEGSIGPKGDLGPRGQSGNPGP------------QGIRGARGQSGPTGEL---------GSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAG------------GSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKG---------IKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            G  GP GL G+P         G+ G+ G +GP+   GPRG  G+PGP             G RG  GQ+GP G+          G KG RG  GPQGP G  G RG                                               G+    G +GP GP GE G  GP G+ G  G  G +G  G +GK          GV G PG PG  G                        +        E  V G  G  GP    G+ G  GP G+ G  GA G+PG KG  G+TG +G RG        GP G  G +G  G  G++GS G+ GP G+ G+    GPPG +G  G  GLPGP+  S       +                   G PG+ G  GPAG              G  GE G  G++GP G AG  G +GP                  +G  GP G  GP G  G  G++GEPGQKG R  KG          +G  G  G  GS GEPGP G  G  G++G+ GP+G  G  GP+G
Sbjct:  512 GPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQ---GPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSV-----------------------------------------GIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKL---------GVPGLPGYPGRQG--------------------PKVTLSSSLIKPPENSVPGTPGKPGPR---GQRGPTGPRGERGPRGATGKPGPKGPTGETGPMGERGHP------GPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGK---DGPPGLRGFTGERGLPGPICFSSLLKTLFSFQ-----------------GSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGP------------------VGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVG 950          

HSP 4 Score: 66.6254 bits (161), Expect = 4.447e-10
Identity = 132/410 (32.20%), Postives = 152/410 (37.07%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGT-------KGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQ---GVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGP----------------KGETGLMGPIGPV 4027
             R    GP GP G  G  GPLG  G  G +GE G  G +G  G  GS GP G  G  G  G  G                             G  G+ G KGD G  G  G PG  G  GP G  G  G  G  GVK          G+ G  GPRGE G +                           GP+   G  GE+G  GP G KG+LG  GLPG  G  GP     +                  PG PGK G +G  G +G +GE G RG  G PG  G  G+ GP G                               RG  G  G PG++G   + G +GS G  G  G  G+ GPPGL G  G+RG PGP                 GE G  GP GP+
Sbjct:  506 ARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGAR---------------------------GMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSVGIFLFHGEIGPPGPRGEDGPE---------------------------GPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKVTLSSSLIKPPENSV--------PGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGPTGETGPMG------------------------------ERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPICFSSLLKTLFSFQGSPGERGAAGPAGPI 823          

HSP 5 Score: 55.4546 bits (132), Expect = 9.381e-7
Identity = 44/97 (45.36%), Postives = 52/97 (53.61%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGET---GPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
             +RG SG  G  G  G +G+ G  G+KG  G+ G QGPRGP+G  G  G  G +G     G RG  G  G +G  G  G  G PG KG RGP G QG
Sbjct:  509 ALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQG 605          

HSP 6 Score: 55.0694 bits (131), Expect = 1.293e-6
Identity = 58/141 (41.13%), Postives = 76/141 (53.90%), Query Frame = 2
Query:  974 GRDGLPGTPGVPGAPGHIFVLPI--------PDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLTGPKGRSGDIGMQGLPGNK 1372
            GR GLPG  G+PG PG + +LP                + + +Q +  +++    L+G  GP G+TG PG  G  G  G+KG+ G  G  G  GP+G  G  G PG  GR+G+DG RG  G TGPKG  G  G+ GLPG K
Sbjct:  455 GRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEK 595          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Xenopus
Match: COL5A1 (collagen type V alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:2209])

HSP 1 Score: 147.517 bits (371), Expect = 8.428e-35
Identity = 271/924 (29.33%), Postives = 371/924 (40.15%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARG---QSGPTGELG---------SKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPV----------------GTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQG---------------EAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGA------VGSKGEPG------PP----------------GLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDA------------LFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            GP G+ GPSGL G+ G  G +G  GP+G  GP G+ G  G  G  GARG   Q+GP G+ G          KG RG  GPQGP G  G RG                                               G+    G +GP GP GE G  GP G+ G  G  G +G  G +GK G         V G PG PG  G          +                      E  V G  G  GP G   + G  GP G+ G  GA G+PG KG  G+TG +G RG        GP G  G +G  G  G++GS G+ GP G+ G+    GPPG +G  G  GLPGP+                G  G +G  G  G PG  G  G  GP G  G PG+ G QGPAG  G QG               E G++G+ G PG  GN G +G           GP G PGP GP+G  G  G  G  G  G+ G  GQKG+   +G  G PG +G       + +  +P       PP                GL GP G++G    KG+ GL GP GP G  GE                  +    +                 I+  P  SS R +RS  A   VD  +A            +F  ++ +  +I++++ P G N+ + ARTC D+ L   +  DG+Y+IDPN+G  +DS +V C F+   ++CI   K+  G      P +   +W +  +  G +    D +   +   QM F++  ++   Q I + C QSV + D+     N +  A   +                   G  D+ +  D    P     +  DGC  +     +  +L+T+ +   PI+D   ND+ +  +   G  V  +CF
Sbjct:  521 GPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSV-----------------------------------------GIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKLG---------VPGLPGYPGRQGPKVTLSSSLIKPP--------------------ENSVPGTPGKPGPRG---QRGPTGPRGERGPRGATGKPGPKGPTGETGPMGERGHP------GPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGK---DGPPGLRGFTGERGLPGPICFSSLLKTLFSFQGSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKG---------EQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQVMPTNIANVSKPTYIYISTPPLCCHRGHVLLELSCLIGLIGPPGEQGE---KGDRGLPGPQGPHGAKGEQVCIFSSHKETSLNDGEHQSPNFEGPPGEV-----------IQPLPIHSSKRTRRSIDASQVVDDGNADNYMDYADGMEEIFGSLNSLKQEIEQMKHPLG-NQQNPARTCKDLLLCHPELPDGEYWIDPNQGCSRDSFKVFCNFTAGGESCIFPDKKSEGARITSWPKENPGSWFSEFK-RGKLLSYVDSNGNPIGVVQMTFMKLLSASVKQNITYNCYQSVAWHDATT---NSYDKAIRFL-------------------GSNDEEMSYDN--NPSIRAIM--DGCASKKGYQKTVLELDTSKVEQAPIVDIMFNDFGDP-VQKFGFEVGPVCF 1310          

HSP 2 Score: 108.227 bits (269), Expect = 8.040e-23
Identity = 62/222 (27.93%), Postives = 100/222 (45.05%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLFQG-KFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTS-------FMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
             DLL A++  +   G      FC   +       AFR      L A ++ LF    FP++FS+L T K +      + SI N+   + + +T G +    Y D            F N+    +L+DG+WHR+AFSV K +++L +DC    ++ L R+  P+  + G +        +  F G +  L++V  A  A   C+ Y PDC+    D   +Q P
Sbjct:   33 ADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNI----NLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDP 250          

HSP 3 Score: 76.2554 bits (186), Expect = 4.338e-13
Identity = 181/559 (32.38%), Postives = 222/559 (39.71%), Query Frame = 2
Query: 2498 GTRGPSGLPGKP---------GIDGKEGSIGPKGDLGPRGQSGNPGP------------QGIRGARGQSGPTGEL---------GSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAG------------GSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKG---------IKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            G  GP GL G+P         G+ G+ G +GP+   GPRG  G+PGP             G RG  GQ+GP G+          G KG RG  GPQGP G  G RG                                               G+    G +GP GP GE G  GP G+ G  G  G +G  G +GK          GV G PG PG  G                        +        E  V G  G  GP    G+ G  GP G+ G  GA G+PG KG  G+TG +G RG        GP G  G +G  G  G++GS G+ GP G+ G+    GPPG +G  G  GLPGP+  S       +                   G PG+ G  GPAG              G  GE G  G++GP G AG  G +GP                  +G  GP G  GP G  G  G++GEPGQKG R  KG          +G  G  G  GS GEPGP G  G  G++G+ GP+G  G  GP+G
Sbjct:  509 GPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQ---GPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSV-----------------------------------------GIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGKL---------GVPGLPGYPGRQG--------------------PKVTLSSSLIKPPENSVPGTPGKPGPR---GQRGPTGPRGERGPRGATGKPGPKGPTGETGPMGERGHP------GPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGK---DGPPGLRGFTGERGLPGPICFSSLLKTLFSFQ-----------------GSPGERGAAGPAGPIGLPGRPGPQGPPGPAGEKGTPGEKGPQGPAGRDGIQGP------------------VGLPGPAGPVGPPGEDGDKGEIGEPGQKGNRGDKGEQGPPGPPGPQGPIGQPGPSGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVG 947          

HSP 4 Score: 65.4698 bits (158), Expect = 8.806e-10
Identity = 132/410 (32.20%), Postives = 152/410 (37.07%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGT-------KGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQ---GVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGP----------------KGETGLMGPIGPV 4027
             R    GP GP G  G  GPLG  G  G +GE G  G +G  G  GS GP G  G  G  G  G                             G  G+ G KGD G  G  G PG  G  GP G  G  G  G  GVK          G+ G  GPRGE G +                           GP+   G  GE+G  GP G KG+LG  GLPG  G  GP     +                  PG PGK G +G  G +G +GE G RG  G PG  G  G+ GP G                               RG  G  G PG++G   + G +GS G  G  G  G+ GPPGL G  G+RG PGP                 GE G  GP GP+
Sbjct:  503 ARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGAR---------------------------GMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSVGIFLFHGEIGPPGPRGEDGPE---------------------------GPKGRGGPSGEIGPLGPGGEKGKLGVPGLPGYPGRQGPKVTLSSSLIKPPENSV--------PGTPGKPGPRGQRGPTGPRGERGPRGATGKPGPKGPTGETGPMG------------------------------ERGHPGPPGPPGEQGLPGVAGKEGSKGDPGPAGLPGKDGPPGLRGFTGERGLPGPICFSSLLKTLFSFQGSPGERGAAGPAGPI 820          

HSP 5 Score: 55.4546 bits (132), Expect = 1.003e-6
Identity = 44/97 (45.36%), Postives = 52/97 (53.61%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGET---GPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
             +RG SG  G  G  G +G+ G  G+KG  G+ G QGPRGP+G  G  G  G +G     G RG  G  G +G  G  G  G PG KG RGP G QG
Sbjct:  506 ALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQG 602          

HSP 6 Score: 55.4546 bits (132), Expect = 1.158e-6
Identity = 58/141 (41.13%), Postives = 76/141 (53.90%), Query Frame = 2
Query:  974 GRDGLPGTPGVPGAPGHIFVLPI--------PDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLTGPKGRSGDIGMQGLPGNK 1372
            GR GLPG  G+PG PG + +LP                + + +Q +  +++    L+G  GP G+TG PG  G  G  G+KG+ G  G  G  GP+G  G  G PG  GR+G+DG RG  G TGPKG  G  G+ GLPG K
Sbjct:  452 GRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEK 592          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Xenopus
Match: COL5A1 (collagen type V alpha 1 chain [Source:HGNC Symbol;Acc:HGNC:2209])

HSP 1 Score: 146.747 bits (369), Expect = 1.060e-34
Identity = 218/772 (28.24%), Postives = 299/772 (38.73%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR-------IGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIA-----------------GNMGQRGPNGPI------------------GIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDA------------LFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            G  GL G  G +GP GP G +G  GPLGK G +G  G  G +G  G+TG  G RG  G+ G PG  G  G                                   G +G IG  GP G   R        GE GPPG  G  G EG  G  G  G+ G LGP GE+G                           + GP G+ G+    GPPG +G  G  GLPGP+G  GP G  G  G  G  G  G  GPVG PG  G  G  G  G  G QG  G++GD GP G A                 G  G  GP G                    GI GP                      G  GL G +G PG++GE+               G +G PGP G  G +G++G  GPKGE+G+ GP GP G  GE                                         I+  P  SS R +RS  A   VD  +A            +F  ++ +  +I++++ P G N+ + ARTC D+ L   +  DG+Y+IDPN+G  +DS +V C F+   ++CI   K+  G      P +   +W +  +  G +    D +   +   QM F++  ++   Q I + C QSV + D   +  N +  A   +                   G  D+ +  D    P     +  DGC  +     +  +L+T+ +   PI+D   ND+ +  +   G  V  +CF
Sbjct:  410 GPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRG---------------------------------PAGPQGPIGYPGPRGVKVRSVGIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKG---------------------------DPGPAGLPGK---DGPPGLRGFTGERGLPGPIGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQVMPTNIANGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVVSSSLKSYDCLSSDPQCVKGYGHPGLIGLIGPPGEQGEK---------------GDRGLPGPQGPHGAKGEQGPTGPKGESGIPGPPGPPGPPGEV----------------------------------------IQPLPIHSSKRTRRSIDASQVVDDGNADNYMDYADGMEEIFGSLNSLKQEIEQMKHPLG-NQQNPARTCKDLLLCHPELPDGEYWIDPNQGCSRDSFKVFCNFTAGGESCIFPDKKSEGARITSWPKENPGSWFSEFK-RGKLLSYVDSNGNPIGVVQMTFMKLLSASVKQNITYNCYQSVAWHD---ATTNSYDKAIRFL-------------------GSNDEEMSYDN--NPSIRAIM--DGCASKKGYQKTVLELDTSKVEQAPIVDIMFNDFGDP-VQKFGFEVGPVCF 1034          

HSP 2 Score: 106.686 bits (265), Expect = 2.480e-22
Identity = 62/222 (27.93%), Postives = 100/222 (45.05%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLFQG-KFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTS-------FMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
             DLL A++  +   G      FC   +       AFR      L A ++ LF    FP++FS+L T K +      + SI N+   + + +T G +    Y D            F N+    +L+DG+WHR+AFSV K +++L +DC    ++ L R+  P+  + G +        +  F G +  L++V  A  A   C+ Y PDC+    D   +Q P
Sbjct:   36 ADLLKALEFHNLPDGIKKTTGFCAYRKSSDGPDVAFRVSKEAQLSAPTNQLFSDVAFPEDFSLLATVKPKKGSHSYLLSIYNEQGTLQIGLTLGWEPMFVYEDHQGHPPPEDYPIFRNI----NLSDGKWHRVAFSVHKKTITLFLDCVKKATLPLKRSDGPIIDTNGIVVFGTRILDEQVFEGDIQQLMIVPDARAAHDYCEHYTPDCDTAIPDAPQSQDP 253          

HSP 3 Score: 72.4034 bits (176), Expect = 7.229e-12
Identity = 157/440 (35.68%), Postives = 181/440 (41.14%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGP-------------LGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPI------------GLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG 3742
            G  GP GL G+PG  G  G  G KG+ G  G  G  GPQG  G  G+ G  G  GS G RG  G  GP GD+G                                               G+ GL G KG  GP GP G  G  GP              G+ G  G RGE G +G +G+ G  G  GP G  GE G PG AG+                   G  G PG  G  GE+G+ G IG  GP G  GR G  GP G  G +G  G PG  G KG  G  GPRG+QGL G  G  G +G  G  G  G Q    N       GE G++GPPG+ G +GP             G+PGPV +S     D     P               G PG  GL GP    G QGE G+RG  GP G  G  G++GP GP G
Sbjct:  392 GPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRG---------------------------------------------FDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSVGIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGDPGPAGL------------------PGKDGPPGLRGFTGERGLPGPIGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQVMPTNI----ANGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVVSSSLKSYDCLSSDPQCVKGY---------GHPGLIGLIGPP---GEQGEKGDRGLPGPQGPHGAKGEQGPTGPKG 752          

HSP 4 Score: 70.4774 bits (171), Expect = 2.573e-11
Identity = 130/383 (33.94%), Postives = 156/383 (40.73%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARG---QSGPTGELG---------SKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTG-----ASGAEGQPGVKGTKGDT------------------GILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGE-----LGIQGPPGRKGELGPIGLPGPVG 3517
            GP G+ GPSGL G+ G  G +G  GP+G  GP G+ G  G  G  GARG   Q+GP G+ G          KG RG  GPQGP G  G RG                                           +G +G  G  G +GP+GP GEKGD GP G  G  G  G  G  G  G  G +G +GPQG +G  G  G  G+             GP GPVG  G  G  G  GE G +G  GL G  G  G  G AGPPG  G      + A G+ G++G  G T                  GI GP     L                    +     +S PQ V+G      +G+ GPPG +GE G  GLPGP G
Sbjct:  404 GPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSVGIFLFHGEIGPPGPRGEDG-----------------PEGPKGRGGPSGEIGPLGPGGEKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGEKGPQGPAGRDGIQGPVGLP------------GPAGPVGPPGEDGDKGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQVMPTNIANGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVVSSSL--------------------KSYDCLSSDPQCVKGYGHPGLIGLIGPPGEQGEKGDRGLPGPQG 737          

HSP 5 Score: 57.3806 bits (137), Expect = 2.761e-7
Identity = 104/324 (32.10%), Postives = 126/324 (38.89%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGI----------DGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDG------------------EKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGV--------SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVK---GTKGDTGILGPRGEQGL 3340
            RGP G +GP G PG  G+           G+ G  GP+G+ GP G  G  GP G  G  G  G  G+ G  G  GK GP G  G  GER                                             G+ G +G KG  GP G DG                  E GD G  G+ G +G +G  G KG EG  G  G  GP G+        +GE G  G  G  G  G QG  G  GP G  G+ G       +    +  D   +   G PG IG  GPPG+ G  G  G PG +   G KG+ G  GP+GE G+
Sbjct:  478 RGPAGPQGPIGYPGPRGVKVRSVGIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGDPGPAGLPGKDGPPGLRGFTGER---------------------------------------------GLPGPIGEKGPQGPAGRDGIQGPVGLPGPAGPVGPPGEDGDKGSDGEPGPRGQQGLFGQKGDEGPRGFAGPPGPVGLQVMPTNIANGESGLEGPPGKTGPIGPQGPPGKPGPEGLRGIPGPVVSSSLKSYDCLSSDPQCVKGYGHPGLIGLIGPPGEQGEKGDRGLPGPQGPHGAKGEQGPTGPKGESGI 756          

HSP 6 Score: 56.6102 bits (135), Expect = 5.119e-7
Identity = 50/113 (44.25%), Postives = 57/113 (50.44%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVK----------GEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G+ G  G KG  GP+G  G  G  G +GVK          GE G  GPRG +G  G KG  G  GE GP G  G KGD G  G PG  G PG++G  G  G  G  GEK
Sbjct:  463 RGFDGLGGLPGEKGHRGPAGPQGPIGYPGPRGVKVRSVGIFLFHGEIGPPGPRGEDGPEGPKGRGGPSGEIGPLGPGGEKGDPGPAGLPGKDGPPGLRGFTGERGLPGPIGEK 575          

HSP 7 Score: 55.4546 bits (132), Expect = 9.330e-7
Identity = 44/97 (45.36%), Postives = 52/97 (53.61%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGET---GPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
             +RG SG  G  G  G +G+ G  G+KG  G+ G QGPRGP+G  G  G  G +G     G RG  G  G +G  G  G  G PG KG RGP G QG
Sbjct:  389 ALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEKGHRGPAGPQG 485          

HSP 8 Score: 55.0694 bits (131), Expect = 1.309e-6
Identity = 58/141 (41.13%), Postives = 76/141 (53.90%), Query Frame = 2
Query:  974 GRDGLPGTPGVPGAPGHIFVLPI--------PDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLTGPKGRSGDIGMQGLPGNK 1372
            GR GLPG  G+PG PG + +LP                + + +Q +  +++    L+G  GP G+TG PG  G  G  G+KG+ G  G  G  GP+G  G  G PG  GR+G+DG RG  G TGPKG  G  G+ GLPG K
Sbjct:  335 GRAGLPGADGLPGPPGTMLMLPFRFSGGGDGGSKGPQVSAQESQAQAILQQARLALRGPSGPMGLTGRPGPLGSPGPSGLKGESGDLGPQGPRGPQGSPGPLGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLGGLPGEK 475          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Mouse
Match: Col27a1 (collagen, type XXVII, alpha 1 [Source:MGI Symbol;Acc:MGI:2672118])

HSP 1 Score: 139.043 bits (349), Expect = 3.086e-32
Identity = 286/771 (37.09%), Postives = 369/771 (47.86%), Query Frame = 2
Query: 2543 GKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKG------------DVGPLGKTGLKGTRG------EVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXX-NSGPQGVEGELGIQGPPGRKGELG---PIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGT------------QGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG---------PVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIY----GDSELSEENKFK 4714
            G  G  G KG  GPRG  G  G QG +G +G+ GP G  G  G +G  G +G +G KG  G PG  GPPG  G  G  G  G  G  G  G +G  GP G +GE G+ G  G KG +GP+GP GE+G            D GP+G  GLKG RG      E G KG EG  G  GS GP G++G PG  G  G  GEKG++G +G++G  G +G  GIPG+PGP G  G KG  G +GPTGAPGR+G  G PG  G +G +G   + G  G  G  G +G+QG DG            G            + G  G EG  G++G PG++G+ G   P G PGP G+ G  G  G PG  G SG+ G  G  G PG  G  G QGP G +G+            QG+ G  GD GP G AG  G  G  G  G  GP G +G+ G  G +GP G  G +G  GL G  GEPG KG+    G  G PG  G  G KG PG  G  G QG RG PG  G+ G++GP G         P G+ G  G  G  GP GPPGP G  G PG         +       +     +G+      S   Q  +D    +F  +  ++N I+ I+ P G  K++ AR C D+    ++  DG Y++DPN G   D+IEV C F++  +TC+  +                     + + E A+SR            QMNFL   +S+  Q I   C    ++    G S   +  +F+
Sbjct: 1039 GLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKG---ITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLG-TKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPI--------------------TASKAEFAVSR-----------VQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFR 1774          

HSP 2 Score: 91.6633 bits (226), Expect = 7.623e-18
Identity = 229/579 (39.55%), Postives = 279/579 (48.19%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDG---KEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGEL------------GSKGDRG------------KFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK------------GDVGPLGKTGLKGTR------GEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLD-XXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGE---LGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGT------------QGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RG PG RG  G  G  G DG   ++GS G KG +GPRG+ G PG QG  G RG SG  G L            G  G+ G            + GP+GP G  GE                                              G+ G  G KG +GP+GP GE+            GD GP+G  GLKG R      GE G KG EG  G  GS GP G++G PG  G  G  GEKG++G +G++G  G +G  GIPG+PGP G  G KG  G +GPTGAPGR+G  G PG  G +G +   G+ G  G  G  G +G+QG D    GP G  G +G  G  G +G  G+ G  G EG  G++G PG++G+    GP G PGP G+ G  G  G PG  G SG+ G  G  G PG  G  G QGP G +G+            QG+ G  GD GP G AG  G  G  G  G  GP G +G+ G  G +GP G  G +G  GL G  GEPG KG+    G  G PG  G  G KG PG  G  G QG RG PG  G+ G++GP G +G  G
Sbjct: 1038 RGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGE---------------------------------------------PGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHK---GITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSG 1568          

HSP 3 Score: 80.1073 bits (196), Expect = 3.123e-14
Identity = 201/620 (32.42%), Postives = 245/620 (39.52%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPG---IDGKEGSIGPKGDL------------GPRGQSGNPGPQGIRGARGQSG------PTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGE---------KGDVGPLGKTGLKGTRGEVGIKGSEGKTGL---------------------------------MGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXS---PGLPGKAGLQGPAGGSGTQGEAG-------------------------------------ERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GPPG  GP G+ GKPG   + G  G +GP+GD             G  G+ G PG  G+ G++G+ G      P GE G  G  G  G  G  G+KG+RG  G  G PGP G  G  G  G  G+ G  G  G PG       +G+ G+ G KG  GP GPDG          KG VGP G+ G  G +G  G +G  G  G                                  MG +GP G  GEPG PGD+                                    G+KGD+G +GP G  G IG+ G PG  G  G  G  G+KG +GD G  G  GE+G +                  G +G  G+ GP G+ G  G +G PG++GE G  G  G  G  G  G+ G PG PG  G  G  G  G+    G PG+ G QG  G +G  G  G                                     +RGDRG PG  G  GQ G  G                               RG  G+ G+PG  G R   G KGS G  G  G  G+ GP G  G QG +G PGP+G  G  GP G  G DG
Sbjct:  901 GPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPG------SRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDS------------------------------------GMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQE------------------GLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQG------------------------------LRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDG 1430          

HSP 4 Score: 71.2478 bits (173), Expect = 1.368e-11
Identity = 114/338 (33.73%), Postives = 143/338 (42.31%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVG 3517
            RG  G PG PG  G+EG  G +G+ G +GQ G+PGP      RG+ GP G  G +G +GK G  GP+G +G                                              +G++GL G +G VG  GP+G  G  G  G+ G  G +G+ G  G  G  G  G RG  GV+G PG+ G                                      G KG+ GL G  G PG+ G     G TG  G +G+PG KG  GD+G +G  G  GL          G KG  G  G +G  G  GP G+ G+ GI GPPG    LGP GLPGP G
Sbjct: 1339 RGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGP------RGRPGPKGSKGEEGPKGKPGKAGPSGRRG---------------------------------------------TQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPP------------------------------------GFKGESGLPGQLGPPGKRGTE---GGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGL---------FGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGM---LGPSGLPGPKG 1574          

HSP 5 Score: 70.8626 bits (172), Expect = 1.633e-11
Identity = 213/568 (37.50%), Postives = 254/568 (44.72%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG---PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ------GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASG------------AEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPP---GRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDR------------GPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXX---------XXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLK---GIKGSPGTIGAVGSKGEPGPPGLTGP------QGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G  GP G PG  G  G++G  GP GD GP+G  G  G  G+ G  G  G  G  G  G RG+ G  G  GD GERG PG  G PG  G  G                             GV GL+G  G++GP+G   P G KG +G +G+ GLKG +GE G+ G  G  G  G +G        G  G+PG  G AG                 G +G  G PG  G  G+ G  GD G  G  G PG  G  GPPG+ G  G             EGQPG KG  G  G+ G +GE G                       G  G  G QG+ G +G+ G P   G KG+ G +G PG  G  G  G  G PG  G  G+ G  GP GS GLPG  G +G  G  G  G AGE+G +            G PG  G  G+RG +G  G +G  GP G PG           GP GP+G PG  G +G  G VGEPG  G+  +K   G  G PG  G +G +GEPG  G  GP      +G RG+PGP GE G  G  G  GEDG
Sbjct:  709 GQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPG---------------------------PPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAG---------------DKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPG---------------------DPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDG 1213          

HSP 6 Score: 70.8626 bits (172), Expect = 1.807e-11
Identity = 48/102 (47.06%), Postives = 63/102 (61.76%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G+ G SG KGD GP G  G +G +G +G  G  G  GP GP+G+ G +GD G  GE G +G++G KG++GS GPPG  G PG +G  G  G +G+ G K
Sbjct: 1141 GEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAK 1242          

HSP 7 Score: 68.1662 bits (165), Expect = 1.243e-10
Identity = 187/514 (36.38%), Postives = 225/514 (43.77%), Query Frame = 2
Query: 2525 GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVE------------GELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXX---------XXXXXXXXXXXXXSRGLDGKVGEPGQKGER---YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG 3994
            G PG+   +   G KG++G  G SGNPGP G +G +G  G     G  G++G+ GP+G                                                     G +G  GR+G  GP+G      D GP G  G  G  G  G+ GS+G+ GL G  G +G  G PG PGD G  G  G  G  G  G                    G  G +GLIG TGA G +G  GP G  G  G  G+PG+KG KG+ G+ G  G+ G  G  G  G  GL GGRGKPG  G  G+ G  G              G  G  GP G KG+ G  GLPGP G  GP G +G  GPPG  G  G  G  G PG PG  G +G  G  G  G  GE+G  G  G+ G  G  G  G  G++GP G  G  G +         G  G  G  GP GSRGL G  G  G +G R      G +GS G  G VG +G PG PG  G  G+RG+ G KG
Sbjct:  661 GDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPG---AAGHPGEQGQPGPEG---------------------------------------------------SPGAKGYPGRQGFPGPVG------DPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGL------------------PGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKG 1096          

HSP 8 Score: 64.6994 bits (156), Expect = 1.371e-9
Identity = 49/109 (44.95%), Postives = 58/109 (53.21%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG------PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + G RG  G  G+KG+ GP G+ G  G  G +G +G  G+ GPRG      PEG AG  G  G  G  G +G  GN GD G VGP G  GNPG+ G  G  G  G  GE
Sbjct: 1371 HPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGE 1479          

HSP 9 Score: 63.1586 bits (152), Expect = 3.492e-9
Identity = 142/400 (35.50%), Postives = 160/400 (40.00%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP---GKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---PGPKGETGLMGPIGPVGEDG 4039
            G  G  G KG  G  G  GE+G  GP G  G KG  G  G  G  G  G  GSRG  G+ G  G PG  G  G                                GV G  G +G  G PG  GE GPP   G  G  G  G PGV G  GDTG LGP G                                GP+G++G +G  G PG KG+ G  G+PG  G        G  G  G  G  G  G  G PG  GKAG +G  G  G  G  G  GD GPPG  G  G +G  G                              +RGL G  G+ G +G+    G  G PG  G  G KG PG PGL G +G+ G+   PGP GE GLMG IG VGE G
Sbjct:  688 GPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGL------------------------------PGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVG------------------------------YPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGD------PGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPG------------------------------ARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPG 991          

HSP 10 Score: 61.2326 bits (147), Expect = 1.413e-8
Identity = 204/589 (34.63%), Postives = 251/589 (42.61%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGK------------PGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGD---------------VGPLGKTGLKGT------------RGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGAS---GAEGQPGVKGTKGDTGI------------LGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER------YLKGIKGSPGTIGAVGSKGE---------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GP G+RG  GLPG             PG+ GK G +G  G  G  G+ G PG  G  G  G  GP G LG  GD G  GP G  G KG +                                    G  G +GE G++G  G +G  G  G  G +GD                GPLGK G KG+             G++G  G  G  G+ G  G +G+ G PG  G  G                 GP+G  G+PG  G  G KG  G  GL G  G PG  G  GP G+ G     G  G+PG+ G KGD G+            +G  G  G  G  G  G  G  G RG PG +G+ G+ GP+G +G  G QG  G KG +GP G PG  G  G +G+ G  G  G  G  G  GP G+ GLPG+ G QGP G  G  GE G +G  G    PG+ G+ G +G  GP+G                        P G +GL G+ GEPG +G+        LKG +G PG  G  G KG+         PGPPG+TG  G+ G PG +GE G  G  G  G  G
Sbjct:  736 GPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMK------------------------------------GLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEG---------------DEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLG------------------------PPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQG 1249          

HSP 11 Score: 60.077 bits (144), Expect = 3.335e-8
Identity = 48/111 (43.24%), Postives = 57/111 (51.35%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP---------PGYAGNPGIKGSRGPVGNQGRDG 2131
            Y G  G++G  G  G  G  G  G RG  G KG KGE G +G  G  G +G +G  G+QG  GPRG  G +G EG+ G          PGY G+ G  G  GPVG  GR G
Sbjct: 1350 YPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRG 1460          

HSP 12 Score: 58.9214 bits (141), Expect = 8.422e-8
Identity = 194/552 (35.14%), Postives = 240/552 (43.48%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---GDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ---GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKV---GEPGQKGERYLKGIKGSPGTIGAV---------GSKGEPGPPGLT---------------GPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G PG  G  G  G+PG +G  G+ G  G  G  G  G+PGP+G RG  G  G  G  GS G+RG  G  G  G+ G                                     PG  G  GE+G  GL G  G +G  GP G     GD G LG  G  G +G  G+ G  G+ GL G +G Q   GVSG+PG  GD G +               G  G+ G  G+PGP G+ G KG +G  GP G  G  G+ GPPG  G  G +G+PG +G  G  G LGP G++         G  G  G  G  G+ G  G  G  G++G  G  G PGR G +G  GL G +G  G  G  G  G  G  G  G  GP GS G PG  G  G  G  G  G  G RG  G  G  G+ G RGP+GP G                         +GS+GL G+V   G PGQ G++   G +G  G  G +         G+KG PG PG                 GP G  G PG  G++G+ G +GP+G  GE
Sbjct:  696 KGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGR------------------------------------PGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSH---------------GLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDE---------GPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAG------------------------EQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGE 1163          

HSP 13 Score: 56.225 bits (134), Expect = 5.460e-7
Identity = 47/103 (45.63%), Postives = 54/103 (52.43%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + GA+G  G  G  G+ GP G+ G +G  G  G  GE   QG  GPEG  G KG  G QG  GP G  G KG  G +G PG  G PG  G RG  G  G+ GE
Sbjct:  671 HDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGE---QGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGE 770          

HSP 14 Score: 54.6842 bits (130), Expect = 1.577e-6
Identity = 45/104 (43.27%), Postives = 58/104 (55.77%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            Y G  G+ G  G+ G+ G  G  G RGE+G  G  G++G    RGP G+ G  G+IG+    GP G  G  GD G++GP GY G  G+KG  G VG  G  G+K
Sbjct:  743 YIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGE---RGPPGLDGNPGEIGL---PGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDK 840          

HSP 15 Score: 54.6842 bits (130), Expect = 1.645e-6
Identity = 44/100 (44.00%), Postives = 53/100 (53.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY---AGNPGIKGSRGPVGNQG 2122
            G RG  G +G  G+ G  G  G  G+ G  G  G  G+ GP+GP G      DIG +G  GPRG  G  G EG +GPPG    +G PG KG RG  G+ G
Sbjct: 1490 GKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPG------DIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLG 1583          

HSP 16 Score: 53.1434 bits (126), Expect = 3.818e-6
Identity = 46/108 (42.59%), Postives = 57/108 (52.78%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQG------ETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA+G  G  G  G  G  G  G+RG +G+ G+ G  G  G RG  G+ G +G++G  G      E GP G DGN G+ G  GPPG  G  G  G+ GPVG  G  G K
Sbjct:  718 GAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMK 825          

HSP 17 Score: 53.1434 bits (126), Expect = 4.874e-6
Identity = 42/103 (40.78%), Postives = 51/103 (49.51%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +KG  G  G  G +G  GP G  G +G  G +G  G  G  GP+G  G  G+ G  G+ G  G RG  G  G  G +G PG+   PG  G RGP G  G  GE
Sbjct:  695 HKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGF---PGDFGERGPPGLDGNPGE 794          

HSP 18 Score: 52.7582 bits (125), Expect = 5.530e-6
Identity = 39/88 (44.32%), Postives = 51/88 (57.95%), Query Frame = 2
Query: 1868 GDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G  GP G VG  G  G  G+KG+ G  GP G +G+ G +G+ G++G+ GP G DG KGD G  GP G  G  G +G +G  G+ G  G
Sbjct: 1132 GPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPG 1219          

HSP 19 Score: 52.373 bits (124), Expect = 7.060e-6
Identity = 198/578 (34.26%), Postives = 242/578 (41.87%), Query Frame = 2
Query: 2486 RGPPGTRGPSG---LPGKPGI---DGKEGSIGPKGDLGPRGQSG------NPGPQGIRGARGQSGPTGELGSKGDRG------------KFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGP------------DGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEP---GSPGDAGVNXXXXXXXXXXXX---------XXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRG------EQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV---------------GSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            RGPPG  G  G   LPG PG+    G  G++GP G  GP+G  G       PG +G +G +G  G +G+ G +GD+G            K GP G  GDKG                 G  G  G  G  G  G  G+ GP+G  G+ G RGL G  G +GP G             +G+ G  G  G+ GL G++GE G  G  G  G  G  G  G+ GEP   G  GD GV G  G  G +GS          G  G  G  G PG  G  G +G KG  G  GP G  G  G  G  G+ G  G  GQPG +G  G+ G  G +G        G  G  G  G  G +G +G  G  G  G  GP G  GE G+ G  G KG+LGP+G PG  G                       G  G PGL G  G  GP G  G +G+ G  G+ G  G  G  G+ G  GP GI G                          G +GK G+ G+KG+R  KG KG  G +G +               GS+G  GP G  G  G +G PG  G  G  G
Sbjct:  783 RGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGS---------------LGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGL---------------------IGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVP------------------------GREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKG 1300          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Mouse
Match: Col24a1 (collagen, type XXIV, alpha 1 [Source:MGI Symbol;Acc:MGI:1918605])

HSP 1 Score: 125.946 bits (315), Expect = 2.690e-28
Identity = 260/824 (31.55%), Postives = 356/824 (43.20%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQ---SGNPGPQGIRG---ARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGK---TGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKG-DTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXX------XXXNSGPQGVEGELGIQGPPGRKGELGPIG---LPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRG-PNGP--IGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYL---KGIKGSPGTIGAVGSKGE---------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXX-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQ--SFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSV---KQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNF-LRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKD 4819
            G PG RG  G PG  G+ G  G +GP G+ GPRG    +G PG  G+ G     G SG  GE G+KGD G  G  G TG+ G RG+PG+ G  G  G +G  G  G  G  G               E G +G +G  G+ GP+G  G+ G  GP G  G  G RG +G KG +G+   TG  GSRGP G  GE G  G  G                                  +G  G +GL+GP G PG  G  G  G+ G SG  G  G KG  G D+ +LGP G  G  GP+G  G  G  G  G  G  G      +TG  GP G  G+ G QGP G +G  GP G   +PGP G                            PG+PG  G  GP G  G  G  G  G  G PG+ G  G  G P  P   G+ G  GP+G  G  GP G  G PGPKG +GL G+ G PGQ+G R     +G++G+PG  G  G  G+         PGP G  G  G  G  GPKG  G  G  GP+G +G  G  G  GP                              R        IR   E +S +   S +    + +  +  +F  +  +++ +  I+ P G  +++ AR C D+   + + +DG+Y+IDPN G   D+ EV C FS   +TC+S V   K + G+   +       + + +  +       P   S + D  ++    +  N + ++E   L  Q V+  D ++ + +  K   +  T +   + +++ + L P+ G   KR  + 
Sbjct:  982 GSPGERGVQGKPGFQGLPGSSGDVGPAGEPGPRGLPGIAGLPGEMGVEGPPGTEGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPGAPGEEGLQGKDGLKGAPGGSGLPG---------------EDGDKGEMGLPGTAGPVGRPGQMGLPGPEGIVGTPGQRGRLGKKGDKGQVGPTGEAGSRGPPGSVGENGPKGARGT---------------------------------RGAVGPLGLMGPEGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPGEDSTVLGPPGPPGEPGPMGEQGETGEHGEEGYKGHMGVPGLRGATGQQGPPGEPGDQGGQGPKGERGSEGPQGKRGVPGPSG---------------------------KPGIPGVPGFPGPKGLQGYPGVDGMSGYPGKPGLPGKQGLLGVPGSPGRTGVAGSPGPQGGKGASGPPGSPGAPGPKGEQGLPGQPGVPGQRGHRGTPGDQGLRGAPGLKGQPGEHGDQGLAGFQGFPGPRGPEGDAGIVGIVGPKGPIGQRGNTGPLGREGIIGPTGGTGPRGEKGFRGETGPQGPRGQPGPPGPPGAPGPRRQMDINAAIRALIESNSAQQMESYQNTEGTLISHSSDIFKTLTYLSSLLSSIKNPLG-TRENPARICKDLLSCQYKVSDGKYWIDPNLGCSSDAFEVFCNFSAGGQTCLSPVSVTKLEFGVSKVQMNFLHLLSSEATHTITIHCLNTPRWSSTWADGPELPISFKGWNGQIFEENTLLEPQ-VLSDDCKIQDGSWHKAKFLFHTQNPNQLPVTEVQNL-PHLGTEQKRYIES 1727          

HSP 2 Score: 99.7525 bits (247), Expect = 2.746e-20
Identity = 196/541 (36.23%), Postives = 242/541 (44.73%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSK---------------GDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGP---AGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGP------PGLTGPQGKRGNP---GPKGETGLMGPIGPVGEDG 4039
            G+RGPSG+ G  G  G  G  G  G  G +G  G+PGP GIRG  G SG  G+ G +               G  G+ GP+GP G  G RG PG  G  G  G  GPIG  G                     E G RG  GRKG +G  GP+G KG+VG  G  G  G  G VG+ G  G TG +G +G +G  G  G  G+ GV                  +G  G PG PGP G  G+ G +G  G  G+PG  G+ GP G  G +G +G  G KG KG+ G    RG  GL G  G                     + G +GV+G+ G QG PG  G++GP G PGP G  G +                        GLPG+ G++GP    G SG QGE G +GD GP G AG  G+ GP G  G                 G  G  G  G +G  G  G PG+ G++   G+ G+ G +G  G  G PGP      PG  G  GK+G+    GP GE G  GP G VGE+G
Sbjct:  724 GSRGPSGIKGDKGEQGVAGEPGEPGYPGDKGNIGSPGPPGIRGKSGPSGQPGDPGPQGPSGPPGPEGFPGDIGIPGQNGPEGPKGHLGNRGPPGPPGLKGTQGEEGPIGPFG---------------------ELGSRGKPGRKGYMGEPGPEGLKGEVGDQGDIGKTGETGPVGLPGEVGITGSIGEKGERGSPGPLGPQGEKGV------------------MGYPGPPGAPGPMGPLGLPGLVGARGAPGSPGPKGQRGPRGPDGLAGDQGGHGAKGEKGNQGK---RGLPGLPGKAG---------------------SPGERGVQGKPGFQGLPGSSGDVGPAGEPGPRGLPGIA------------------------GLPGEMGVEGPPGTEGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPG---------------APGEEGLQGKDGLKGAPGGSGLPGEDGDKGEMGLPGTAGPVGRPGQMGLPGPEGIVGTPGQRGRLGKKGDKGQVGPTGEAGSRGPPGSVGENG 1162          

HSP 3 Score: 94.7449 bits (234), Expect = 9.895e-19
Identity = 208/617 (33.71%), Postives = 255/617 (41.33%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGS------------------------IGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG------------------------------------------------IKGSEGKTGLMGSRGPQGVSGEPGS---PGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA------------------GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGP------PGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGP G  GP G PG PG+ G +G                         +GP G  G +G  G+PG  G+RG  G  G  G +GS G  G+ G  GP G+ G                                              KGVRG +G  G VG +GP+GE+G  G  GK G KG +G  G                                                + GS G +G+ G +G QGV+GEPG    PGD G  G  G  G +G  GP+G  G  G  G  GP G +G  GDIG+      PG+ G  GP G  G  G  G PG+KGT+G+ G +GP GE G                RGKPGR+G  G  GP+G++GE+G QG  G+ GE GP+GLPG VG                          GS G  G+ G  GP G  G +G                    G  G RG PG  G  GQRGP GP G+ G  G  G  G  G  G  G P      GL GK G PG++G +   G +G PG+ G VG  GEPGP       GL G  G  G PG +G++GL G  G  G+ G
Sbjct:  531 RGPRGIPGPHGNPGLPGLPGPKGPKGDPGLSPGQAASGEKGDPGLLGLVGPPGLQGAKGLKGHPGLPGLRGEHGLPGLAGNIGSPGYPGRQGLAGPEGNPGS---------------------------------------------KGVRGFIGSPGEVGQLGPEGERGTPGVRGKKGPKGRQGFPGDFGDRGPAGLDGSPGLVGGTGPPGFPGVTGSVGPAGPTGPPGAPGPMGLSGSRGPSGIKGDKGEQGVAGEPGEPGYPGDKGNIGSPGPPGIRGKSGPSGQPGDPGPQGPSGPPGPEGFPGDIGI------PGQNGPEGPKGHLGNRGPPGPPGLKGTQGEEGPIGPFGELG---------------SRGKPGRKGYMGEPGPEGLKGEVGDQGDIGKTGETGPVGLPGEVGI------------------------TGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGAPGPMGPLGLPGLVGARGAPGSPGPKGQRGPRGPDGLAGDQGGHGAKGEKGNQGKRGLP------GLPGKAGSPGERGVQGKPGFQGLPGSSGDVGPAGEPGPRGLPGIAGLPGEMGVEGPPGTEGDSGLQGEPGAKGDGG 1051          

HSP 4 Score: 87.0409 bits (214), Expect = 1.990e-16
Identity = 204/536 (38.06%), Postives = 235/536 (43.84%), Query Frame = 2
Query: 2519 LPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDG------------EKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXS----------------PGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            LPG  G  G  GS GPKG  GPRG      P G+ G +G  G  GE G++G RG  G  G  G  GERG  G                                       + G +GL G  G VGP G  G            E G  GP G  G  G +GE G KG  G  G  G+ G  G  GEPG+PG+ G+ G+ G +G  G  G  G               E G KG++GL G  G  GR G+ G PG  G  G  GQ G  G KGD G +GP GE G  GP G +G  G KG RG  G  G  G  GP   EGE GI G  G +G+ GP GLPGP G  G  G+D     P G                            PGL G  G QGP G  G QG  G +G+RG  G  G  G  GP+G  GI         PG  G  GP G  G  G  G+ G  G+PG  G++ L G+ GSPG  G  GS G  G  G +GP G  G PGPKGE GL G  G  G+ G 
Sbjct:  926 LPGLVGARGAPGSPGPKGQRGPRG------PDGLAGDQGGHGAKGEKGNQGKRGLPGLPGKAGSPGERGVQG---------------------------------------KPGFQGLPGSSGDVGPAGEPGPRGLPGIAGLPGEMGVEGPPGTEGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPGAPGEEGLQGKDGLKGAPGGSGLPG---------------EDGDKGEMGLPGTAGPVGRPGQMGLPGPEGIVGTPGQRGRLGKKGDKGQVGPTGEAGSRGPPGSVGENGPKGARGTRGAVGPLGLMGP---EGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPGEDSTVLGPPGPPGEPGPMGEQGETGEHGEEGYKGHMGVPGLRGATGQQGPPGEPGDQGGQGPKGERGSEGPQGKRGVPGPSGKPGI---------PGVPGFPGPKGLQGYPGVDGMSGYPGKPGLPGKQGLLGVPGSPGRTGVAGSPGPQGGKGASGPPGSPGAPGPKGEQGLPGQPGVPGQRGH 1389          

HSP 5 Score: 85.1149 bits (209), Expect = 9.309e-16
Identity = 184/546 (33.70%), Postives = 221/546 (40.48%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGK---EGSIGPKGDLGPRGQSGNPGPQGI------RGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTG------LKGTRGEVG---IKGSEGKTGLMGSRGPQGVSGEPGSPG----------DAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILG------PRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA---------GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG 3994
             GPPGT G SGL G+PG  G     GS G  G+ GPRG+ G PG +G+      +GA G SG  GE G KG+ G  G  GP G  G+ G PG +G  G                                   G RG LG+KG  G +GP GE G  GP G  G       +GTRG VG   + G EG+ G+ G RG QG  G  G PG          D+ V G  G  G+ G  G  G  G  G  G  G  G  G++G  G  GP G PG  G  GP G+ G+ G +G+ GV G  G  GI G      P+G QG                                GV+G  G  G PG  G+ G +G+PG                              SPG  G AG  GP GG G  G           GE+G  G PG+ G  G RG  G  G+                        +G+ GL G+ GE G +G   L G +G PG  G  G  G  G  G  GP G+RGN GP G
Sbjct: 1029 EGPPGTEGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPGAPGEEGLQGKDGLKGAPGGSGLPGEDGDKGEMGLPGTAGPVGRPGQMGLPGPEGIVGT---------------------------------PGQRGRLGKKGDKGQVGPTGEAGSRGPPGSVGENGPKGARGTRGAVGPLGLMGPEGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPGEDSTVLGPPGPPGEPGPMGEQGETGEHGEEGYKGHMGVPGLRGATGQQGPPGEPGDQGGQGPKGERGSEGPQGKRGVPGPSGKPGIPGVPGFPGPKGLQGYP------------------------------GVDGMSGYPGKPGLPGKQGLLGVPG------------------------------SPGRTGVAGSPGPQGGKGASGPPGSPGAPGPKGEQGLPGQPGVPGQRGHRGTPGDQGL------------------------RGAPGLKGQPGEHGDQG---LAGFQGFPGPRGPEGDAGIVGIVGPKGPIGQRGNTGPLG 1454          

HSP 6 Score: 61.2326 bits (147), Expect = 1.600e-8
Identity = 45/109 (41.28%), Postives = 64/109 (58.72%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP------GYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G +G  G+ GD GP+G+ G RG  G+ G+ GE G++GP G EG +G++G+ G +G+ GP G  G  G+ G  G P      G  G  G+KG+ G  G  G DG+K
Sbjct:  990 QGKPGFQGLPGSSGDVGPAGEPGPRGLPGIAGLPGEMGVEGPPGTEGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPGAPGEEGLQGKDGLKGAPGGSGLPGEDGDK 1098          

HSP 7 Score: 59.6918 bits (143), Expect = 4.665e-8
Identity = 46/102 (45.10%), Postives = 57/102 (55.88%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G RG++G  G +G  G SG VG  GE G +G+ G  G+ G  G EG  G +GD G+QGE G +G  G  G  G+ G PG  G PG  G     G QG+DG K
Sbjct:  985 GERGVQGKPGFQGLPGSSGDVGPAGEPGPRGLPGIAGLPGEMGVEGPPGTEGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPGAPGEE---GLQGKDGLK 1083          

HSP 8 Score: 59.3066 bits (142), Expect = 5.429e-8
Identity = 45/103 (43.69%), Postives = 60/103 (58.25%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G++G  GAKGD GP+G  G  GE G +G  G  G +G +G +G+ G  G  G+ GE G +G+ G  G  G VG PG  G PG +G  G  G +GR G+K
Sbjct: 1035 EGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPGAPGEEGLQGKDGLKGAPGGSGLPGEDGDKGEMGLPGTAGPVGRPGQMGLPGPEGIVGTPGQRGRLGKK 1137          

HSP 9 Score: 58.5362 bits (140), Expect = 8.999e-8
Identity = 49/105 (46.67%), Postives = 59/105 (56.19%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGI---AGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GARG  GS G KG  GP G  G  G+ G  G KGE G QG RG  G+   AG  G+ G+QG+ G +G  G+ GD G  G PG  G PGI G  G +G +G  G +
Sbjct:  931 GARGAPGSPGPKGQRGPRGPDGLAGDQGGHGAKGEKGNQGKRGLPGLPGKAGSPGERGVQGKPGFQGLPGSSGDVGPAGEPGPRGLPGIAGLPGEMGVEGPPGTE 1035          

HSP 10 Score: 57.3806 bits (137), Expect = 2.391e-7
Identity = 46/111 (41.44%), Postives = 60/111 (54.05%), Query Frame = 2
Query: 1847 RGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGI------QGETGPRGKDGNK---------GDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + +SG KGD G  G VG  G  G KG+KG  G+ G RG  G+ G+ G+IG       QG  GP G  G+K         G+ G +GP G  G PG++G +GP G QG  G+
Sbjct:  564 QAASGEKGDPGLLGLVGPPGLQGAKGLKGHPGLPGLRGEHGLPGLAGNIGSPGYPGRQGLAGPEGNPGSKGVRGFIGSPGEVGQLGPEGERGTPGVRGKKGPKGRQGFPGD 674          

HSP 11 Score: 54.6842 bits (130), Expect = 1.474e-6
Identity = 45/102 (44.12%), Postives = 55/102 (53.92%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            YKG  G+ G  GA G  GP G+ G +G  G KG +G  G QG RG  G +G  G  G+ G  GP+G  G  G +G  G PG  G PG +G  G  G+ GR G
Sbjct: 1245 YKGHMGVPGLRGATGQQGPPGEPGDQGGQGPKGERGSEGPQGKRGVPGPSGKPGIPGVPGFPGPKGLQGYPGVDGMSGYPGKPGLPGKQGLLGVPGSPGRTG 1346          

HSP 12 Score: 54.299 bits (129), Expect = 1.745e-6
Identity = 43/96 (44.79%), Postives = 56/96 (58.33%), Query Frame = 2
Query: 1844 IRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            ++G+ G +G  GP G++G+RG+ G KG  GE    GP G +G  G +GDIG  GETGP G  G  G  GS+G  G  G+P      GP+G QG  G
Sbjct:  821 LKGTQGEEGPIGPFGELGSRGKPGRKGYMGE---PGPEGLKGEVGDQGDIGKTGETGPVGLPGEVGITGSIGEKGERGSP------GPLGPQGEKG 907          

HSP 13 Score: 51.9878 bits (123), Expect = 9.973e-6
Identity = 41/103 (39.81%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G+ G +G+ G+ G  G+ G +G  G  G  G  G  GPRG  GIAG+ G++G++G  G  G  G +G+ G+ G  G AG+ G  G  GP G  G  GE+
Sbjct:  972 RGLPGLPGKAGSPGERGVQGKPGFQGLPGSSGDVGPAGEPGPRGLPGIAGLPGEMGVEGPPGTEGDSGLQGEPGAKGDGGPAGSAGATGEPGPRGEPGAPGEE 1074          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Mouse
Match: Col11a1 (collagen, type XI, alpha 1 [Source:MGI Symbol;Acc:MGI:88446])

HSP 1 Score: 113.62 bits (283), Expect = 1.918e-24
Identity = 68/262 (25.95%), Postives = 117/262 (44.66%), Query Frame = 2
Query: 4226 FVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            + D  + +F  ++ +   I+ ++FP G  + + ARTC D++L      DG+Y+IDPN+G   DS +V C F+   +TCI   K+  G+     P +   +W +  +  G +    D+    ++  QM FL+   + + Q   + C QS  + D             VL     K++       LG N+  M    Y++       ++   YDGC  R     +  ++ T  +  +PIID  IND+ ++     G  V   CF
Sbjct: 1570 YSDGMEEIFGSLNSLKQDIEHMKFPMG-TQTNPARTCKDLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRISSWPKEKPGSWYSEFK-RGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSAAWYD-------------VLSGSYDKALRF-----LGSNDEEMS---YENN-----PHIKALYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQN-QKFGFEVGPACF 1802          

HSP 2 Score: 104.76 bits (260), Expect = 7.919e-22
Identity = 58/207 (28.02%), Postives = 101/207 (48.79%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLFQG-KFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPS-STSFMNLPF--EFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPL-DISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCN 853
            VD+L A+   +  +G +    FC N +       A+R      + A +  LF G  FP++FSIL T K +      + S+ N++    L V  GR     + D +   +  N P     ++ DG+WHR+A SV+K +V++++DC+   +  L+R+   + D +G  ++  +    D  F G +   L+ G    A+  C  Y+PDC+
Sbjct:   38 VDILKALDFHNSPVGISKTTGFCTNRKNSKDPDVAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFEDHTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRILETDV-FQGDIQQFLITGDPKAAYDYCDHYSPDCD 243          

HSP 3 Score: 70.8626 bits (172), Expect = 1.892e-11
Identity = 51/103 (49.51%), Postives = 63/103 (61.17%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G+RG  G+KG+ G  G  G +G++G+KG +GE G  GPRG +G  G KG  G  G+ GP G+ G KG  G  G PGY G  G KGS G  G  G +GEK
Sbjct:  763 KGADGVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEK 865          

HSP 4 Score: 60.4622 bits (145), Expect = 2.496e-8
Identity = 50/111 (45.05%), Postives = 58/111 (52.25%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP---------GYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G +G  G KGD G  GQVG RGE G +G KG  G  G  GP G AG KG +G+ G  G  G+ G KG  G  G P         G AG PG +G RGP G +G  G +
Sbjct:  782 GFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGAR 892          

HSP 5 Score: 55.8398 bits (133), Expect = 6.124e-7
Identity = 187/460 (40.65%), Postives = 215/460 (46.74%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGP---------DGEKGDVG------------------PLGKTGLKGTRGEVGIKGSEGKTGLM---GSRGPQGVSGEP---------------GSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNG 3733
            GP G  G  G  G PG+ G  G  GPKG  G  G  G  G +G RG  G+ GP G+ G  G RG  G +GPTG  G +G  G  GPPGP G  GP G  G  G  G KG  G  G  G  G  G RG  G +G  GP GP          GE G +G                    GK G KG  G  GI G +G  G+    G RG  G  G P               GSPG+ G  G  G  G  G  GP GP G  G  G PG +G +G  G  G+ GP G PG  G AG PG+ G  G  G+PG KG+KGD      +GE G  GP G  G  G  G  G  G  G  G  G  G +G+ G +G PG  G +G  GLPGP G  G +G  G  GPPG  G  G  GP G+ G  G  G  G  G  G +GE GE G+ GPPG AG+ G +G  G
Sbjct:  824 GPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGPQGPVGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDKGEIGEPGQKGSKGD------KGENGPPGPPGLQGPVGAPGIAGGDGEPGPRGQQGMFGQKGDEGARGFPGLPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGVVGDKGEPGEAGNPGPPGEAGSGGLKGERG 1277          

HSP 6 Score: 55.4546 bits (132), Expect = 9.732e-7
Identity = 42/93 (45.16%), Postives = 52/93 (55.91%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIK 2092
            G  G +G +G  GD GPSGQ G +G++G+ G+ G  G QGP+G  G  G       KG  GI G+ GPRG+ G  G  GS G  G  G PG K
Sbjct:  809 GPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPK 901          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Mouse
Match: Col5a1 (collagen, type V, alpha 1 [Source:MGI Symbol;Acc:MGI:88457])

HSP 1 Score: 111.309 bits (277), Expect = 9.923e-24
Identity = 64/219 (29.22%), Postives = 98/219 (44.75%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRRK-----AFRYESGNSLMAKSDVLF-QGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGP 889
             DLL  +   +   G T    FC   R       A+R      L   +  L+ +  FP++FSIL T K++      + SI N+     L +  GR     Y D    P    +   P   +L+DG+WHRIA SV K +V+L++DC+   +  L+R+  P+  + G +        D  F G +  LL V     A+  C+ Y+PDC+    D   +Q P
Sbjct:   40 ADLLEMLDFHNLPSGVTKTTGFCATRRSSSEPDVAYRVSKDAQLSMPTKQLYPESGFPEDFSILTTVKAKKGSQAFLVSIYNEQGIQQLGLELGRSPVFLYEDHTGKPGPEEYPLFPG-INLSDGKWHRIALSVYKKNVTLILDCKKKITKFLSRSDHPIIDTNGIVMFGSRILDDEIFEGDIQQLLFVSDNRAAYDYCEHYSPDCDTAVPDTPQSQDP 257          

HSP 2 Score: 107.457 bits (267), Expect = 1.426e-22
Identity = 65/267 (24.34%), Postives = 116/267 (43.45%), Query Frame = 2
Query: 4220 QSFVDINDAL---FDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            +S+VD  D +   F  ++ +  +I++++ P G  + + ARTC D++L      DG+Y++DPN+G  +DS +V C F+    TC+   K+  G      P +   +W +  +  G +    D     V   QM FLR  ++ ++Q + + C QSV + D+     +  K    L ++D               E   D   Y          +    DGC  +     +  +++T  +  +PI+D   ND+  +     G  V   CF
Sbjct: 1599 ESYVDYADGMEEIFGSLNSLKLEIEQMKRPLG-TQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFK-RGKLLSYVDAEGNPVGVVQMTFLRLLSASAHQNVTYNCYQSVAWQDAATGSYD--KAIRFLGSND--------------EEMSYDNNPY----------IRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFNDF-GEASQKFGFEVGPACF 1836          

HSP 3 Score: 68.1662 bits (165), Expect = 1.050e-10
Identity = 52/103 (50.49%), Postives = 61/103 (59.22%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA GIRG  G KG+ G  G  G +G++G+KG +GE G  GPRG +G  G KG  G  G+ GP G  G KG  G  G PGY G  G KGS G  G  G +GEK
Sbjct:  795 KGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEK 897          

HSP 4 Score: 52.7582 bits (125), Expect = 5.669e-6
Identity = 41/95 (43.16%), Postives = 50/95 (52.63%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGN 2116
            G  G +G  G  GD GP G  G +G++G+ G+ G  G QGP+G  G  G  G  G +G  G  GK G +G  G  GP G  G  GI G  GP GN
Sbjct:  841 GPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGN 935          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Mouse
Match: Col5a3 (collagen, type V, alpha 3 [Source:MGI Symbol;Acc:MGI:1858212])

HSP 1 Score: 109.383 bits (272), Expect = 3.781e-23
Identity = 136/484 (28.10%), Postives = 215/484 (44.42%), Query Frame = 2
Query: 3605 GLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPIG------IVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGP---IGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVK--QQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCFS 5014
            GLPGK G  G  G  G +GE G +GD GP   PG  G +G RGP G IG      I GP G  G  GP G +GP G  GP G  GL G  G  G+KG   L G+ G PG  G  G +G PG  G  G QG  G PGP G  G  GP   +GP+G+ G  G+ G LGP G PGP G  G PG   +                       G  +R +   +     + +   ++ ++ ++++++ P G   +S    C ++        DG+Y+IDPN+G  +D+ +V C F+   +TC+   K  +   L ++ +   G   W ++ R     S   D     V+  Q+ FL+  ++ ++Q   ++C  SV + D    +    +++      +++ +  ++                          + + +DGC  R   + + F+  ++ +  LP+ D   +D+        G  + S+CFS
Sbjct: 1306 GLPGKRGSPGRMGPEGREGEKGAKGDAGPDGPPGRTGPIGARGPPGRIGPDGLPGIPGPVGEPGLLGPPGLIGPPGPLGPPGLPGLKGDAGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKGSKGSPGSLGPRGDPGPAGPPGPPGSPAEV---------------------HGLRRRRSVTDTLEGGLEEVMASLNSLSLELQQLQRPLG-TAESPGLMCRELHRDHPHLPDGEYWIDPNQGCARDAFKVFCNFTAGGETCLYPDKKFETVKLASWSREKPG--GWYSTFRRGKKFSYV-DADGSPVNVVQLTFLKLLSAAAHQRFTYICQNSVAWLDEAAGDH---RHSIRFQGTNWEELSFNQTT---------------------AATIKVSHDGCRVRKGQAKTLFEF-SSSVGFLPLWDVAASDFGQTN-QKFGFELGSICFS 1738          

HSP 2 Score: 89.7373 bits (221), Expect = 2.865e-17
Identity = 57/204 (27.94%), Postives = 89/204 (43.63%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCG---NSRRKAFRYESGNSLMAKSDVLF-QGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVL--QDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNN 856
            VD+L A  +     G      FC        +AFR    + L   +  LF  G FP+ FS+L+T +++  +   + SI ++       V                SF  LP + ++ DG+WHR+A S+  + V+L++DC+            P  IS   L V+  QD   + SF G +  LLL+     AF+ C+ Y P C  
Sbjct:   33 VDVLEAWGVHRDQAGVAEGPGFCPLRIPQGDRAFRVGKSSLLSVPTWQLFPDGHFPENFSVLLTLRAQPANQSVLLSIYDEK-----GVRQLGLALGPALGLLGDSFRPLPKQVNIMDGRWHRVAVSISGNKVTLVVDCE---PQPPTFGQGPRFISTAGLTVMGTQDTR-EESFEGDIQELLLIPDPQAAFQACESYLPGCET 227          

HSP 3 Score: 85.8853 bits (211), Expect = 4.787e-16
Identity = 215/534 (40.26%), Postives = 269/534 (50.37%), Query Frame = 2
Query: 2501 TRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGV------KGDIGLIGPTGAPGRIGEAGPPGKTG---------------ASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGP---IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQR---GPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            + GP G PG  G  G++G+ GP G  GPRG  G      +RG +G SG  G  G KG+RG+ G  G  GD+G +GD G+ GPPGP G +GP G  G  G  G +G  G  G +G   + GV GL G  G  GP G  G  G +GPLG+ G +G  G+ G +G  G  G  G RG  G +G+PG  GD G NG  G  G++G  G  GP G  G  G PGP+G+ G+      +G++G  G TG PG  G  GP GK G                 G +G PG++G +G  G LGP G  G +GP GP G  G +G  G PG  G  G+ GP    G +G  G PG +G +GP   IGLPG  G  GP G  G  G PG  G  G  G     G+PG  GLQGP+G +G  GE G++G+ G PG  G+ G +   GP GP GI                           RG  G  G PG  G    +G +G PG  G  G  G  G  G+ GP G +G PGP GE G +G +G
Sbjct:  677 SEGPPGHPGHEGPTGEKGAQGPPGSAGPRGYPG------LRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKG---KLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGPRGFPGPQGAPGDPGPIGLKGDKGP---PGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKD---GIPGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGI---------------------------RGPAGHSGLPGADG---AQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVG 1165          

HSP 4 Score: 82.8037 bits (203), Expect = 4.178e-15
Identity = 202/565 (35.75%), Postives = 236/565 (41.77%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELG------------SKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGL---------------------QGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER---------YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGL------MGPIGPVGEDG 4039
            GPPG  G  GL G PG  G+ G  GP+G +GPRG  G  G  G+ G+ G  G  G +G            + G +G  GPQGP G  GE+G PG+ G PG  G                                      G  G  G  GP GEKG  GP G  G +G  G  G+KG+ G  GL G +G +G  G PG  GD G                 G  G  G PG  G  G +G KG  GL G  G PG  GE G  G  G  G  G+PG KG+ G  G LGP GE+G                          G +G  G EGE G  G  G +G+ G  G PGP G                       G  GSPG PG+ GL                      G +G  G +GE G +G  GPPG AG +   GP G +G VGP G RG PGP GP G  G PG +G  G  G++G  G  G+            +G  G PG IG  G KG PGP G  G  G+RG  GP G  GL       GPIGP GE G
Sbjct:  574 GPPGEDGVKGLQGPPGPTGQAGEPGPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSE------------------------------------GPPGHPGHEGPTGEKGAQGPPGSAGPRGYPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPK---------------GDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKG------------------------KRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGD---------------------VGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAGVL---GPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGPRGFPGPQGAPGDPGPIGLKGDKGPPGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKG 1039          

HSP 5 Score: 79.7221 bits (195), Expect = 3.835e-14
Identity = 159/463 (34.34%), Postives = 197/463 (42.55%), Query Frame = 2
Query: 2486 RGPPGTRGPSG------LPGKPGIDGKEGSIGPKGDLGP------RGQSGNPGPQG------IRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKT------------------GLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGP------PGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIV 3748
             GPPG  G  G      LPG PG  G +GSIG  G LGP      RG++G PG +G       RG RGQ G TG+ G KGD G+ G  GP G+KG              G  GP G  G  G  G +G  G  G  G  GE G +GL G  G  G +GP G+ GDVGPLG+                   G +G +GE+G  GS GK G  G RG  G  G PG PG  G+                                 KG KG  G +G  G+PG  G  GP      PG++G  G  G  G KG+ G+ G  GP G+ G+                            GP G++G  G  GP G +G+ G +G+PG  G+ G  G                       G PG  G++GPAG SG  G  G +G RGPPG+ G  G  G  G +G++
Sbjct:  774 EGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKG------------LPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIEGREGAKGELGPLGSVGKEGPPGPRGFPGPQGAPGDPGPIGL---------------------------------KGDKGPPGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKDGI---------------------------PGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDA---------------------GPPGPTGIRGPAGHSGLPGADGAQGRRGPPGLFGQKGDDGVRGFVGVI 1143          

HSP 6 Score: 70.0922 bits (170), Expect = 2.936e-11
Identity = 150/411 (36.50%), Postives = 180/411 (43.80%), Query Frame = 2
Query: 2882 KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGS------------EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGEL------------GPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            KG  GP+G  G  G VG  G  GLKG  GEVG +G             EGKTG  G+ G +G+ G+ G  GD G +                        G PG  GEKG +GD G +G  G PG  G  G  G  G +G  G+PG +G  G  G+ GP G  G+ G  G  G+KG  G  G+PG  G  GN G QG+ G  G  G PG KG              GP G PG  G +G  G  G PG  G              G PG  G++G +G  G QGE GERG+ G PG  G+ G +                           G+ G  G  G  G+ G  GQKG   L G +G PG  G  G  G PG PG        G PGPKG  G  GP+GP+GE G+
Sbjct:  477 KGPPGPVGLTGRPGPVGLPGYPGLKGELGEVGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGPKGDRGFD------------------------GLPGLPGEKGQRGDFGRVGQPGPPGEDGVKGLQGPPGPTGQAGEPGPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQGPPGSAGPR------------GYPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPK---------------------------GDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKGKLGVPGLPGYP------GRPGPKGSIGFPGPLGPLGEKGK 818          

HSP 7 Score: 65.855 bits (159), Expect = 5.650e-10
Identity = 198/590 (33.56%), Postives = 232/590 (39.32%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPG---KPGIDGKEGSIGPKGDLGP------------RGQSGNPGPQGIRGARGQ---------------SGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGP------------LGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGA------------SGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ------------------GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            GP G  GP GLPG   +PG+ G +G+ G KG++GP             G  G PGPQG  G  G+                GP G  G +G  G+ G QGP G  G RG PG +G  G                                   G RGL G KG  G  G  G KGD GP             G+ G +G +G  G+ G EG  G  G +G  GV G PG PG  G                                  KG  G  G +GP G  G+ G+AG PG+ G              GA GQPG KG  G  G  GP GE+GL G  GP G  G KG  G  G+ G +G+ G +G  G  G+ GPPG  G LGP G  G VG  G  G  G PGPPG  G  G +      G  G  G  GP G  G +                  G  G +GD+GPPG  G  G  G  GP+G                                G +G PGQ G +         G IG  G KG PG  G  GP GK G PGP G  G  G  GP GE+G+
Sbjct:  598 GPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQGPPGSAGPRGYPGLRGVKGT---------------------------------SGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKGKLGVPGLPGYPGRPG---------------------------------PKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEQGLPGIE------GREGAKGELGPLGSVGKEGPPGPRGFPGPQGAPGDPGPIGLKGDKGPPGPVGANGSPGERGPVGP------------------------------SGGIGLPGQSGGQ---------GPIGPAGEKGSPGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEEGD 1076          

HSP 8 Score: 62.3882 bits (150), Expect = 5.877e-9
Identity = 188/572 (32.87%), Postives = 221/572 (38.64%), Query Frame = 2
Query: 2486 RGPPGT------RGPSGLPGKPGIDGKEGSIGPKGDLGP---------RGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEK---------------GVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGI---QGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTG---------------------PQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            +GPPG        GP GLPG PG+ G+ G +GP+G  G           G++G  G  G RG  G +GP      KGDRG  G  G  G+KG+                                                RG  GR G  GP G DG KG  GP G T   G  GE G +G  G  GL G  G  GV+G  G+PG  G  G  GE G  G QG  G  G+ G  G  G  GEK               G  G  G  GPT   G  G  GPPG  G  G  G  GVKGT G+ G+ G +GE+G DG  G  G +G KG RG PG  G  G  GP+G +G  G+   +GPPG  GE G +G+PG                               PG PG+ G +G  G  G  G  GE+G RG  G  G  G+R                                          G PG +G+R   G  G PG  G VG  G PGPPG  G                       G  G+PG +GE G  G  GP G  G
Sbjct:  477 KGPPGPVGLTGRPGPVGLPGYPGLKGELGEVGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGP------KGDRGFDGLPGLPGEKGQ------------------------------------------------RGDFGRVGQPGPPGEDGVKGLQGPPGPT---GQAGEPGPRGLIGPRGLPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPT---GEKGAQGPPGSAGPRGYPGLRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGPPGPRGEDGPEGQKGPGGLPGDEGPPGAAGEKGKLGVPG------------------------------LPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGER------------------------------------------GTPGTRGDRGQPGATGQPGPKGDVGQNGSPGPPGEKGLPGLQGPPGFPGPKGPPGPQGKDGISGHPGQRGELGFQGLTGPPGPAG 916          

HSP 9 Score: 58.9214 bits (141), Expect = 7.233e-8
Identity = 52/112 (46.43%), Postives = 63/112 (56.25%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGM---KGVKGEYGIQGP------RGPEGIAGVKGDIGIQGETGPRGKDGNKGDE------GSVGPPGYAGNPGIKGSRGPVGNQG 2122
            G  G++G SGA G   PSG+ G +GEVGM   KG KG+ G  GP      RGP G +G+ G  G QG  GP G  G KGD+      G +GPPG  G PG  G +G VG+ G
Sbjct: 1057 GPPGLQGPSGAAG---PSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHSGLPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVG 1165          

HSP 10 Score: 57.3806 bits (137), Expect = 2.352e-7
Identity = 47/105 (44.76%), Postives = 60/105 (57.14%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGP---RGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G+ G SG +G  GP+G+ G+ GE G  G  G+ GI GP   +GP G AG  G+ G +GE G  G  G+KGD+G  GPPG     GI+G  G  G  G DG +
Sbjct: 1018 GGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPT---GIRGPAGHSGLPGADGAQ 1119          

HSP 11 Score: 55.0694 bits (131), Expect = 1.018e-6
Identity = 73/168 (43.45%), Postives = 90/168 (53.57%), Query Frame = 2
Query: 2861 VRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---------AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
             RGL G++GS G +GP+G +G+         KG +G+ G  G  G+TG +G+RGP G  G  G PG  G  GE G  G  G  GP GP+G  G+PG  G  G KG KG IGLIG  G PG  GE          GPPG  G  G  G  G  G  G  G++GP G++G
Sbjct: 1304 ARGLPGKRGSPGRMGPEGREGE---------KGAKGDAGPDGPPGRTGPIGARGPPGRIGPDGLPGIPGPVGEPGLLGPPGLIGPPGPLGPPGLPGLKGDAGPKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKG 1462          

HSP 12 Score: 54.6842 bits (130), Expect = 1.552e-6
Identity = 45/103 (43.69%), Postives = 56/103 (54.37%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSG------QVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            G  G  GS G +G  GPSG      Q G +G +G  G KG  G +G  GP G  G+ G  G+QG +G  G  G +GD+G VG PG+ G+ G KG  GP G  G
Sbjct: 1000 GPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTG 1102          

HSP 13 Score: 53.5286 bits (127), Expect = 2.918e-6
Identity = 45/109 (41.28%), Postives = 54/109 (49.54%), Query Frame = 2
Query: 1838 RGIRGSSGAKGDHGPSGQVGTRGEVGMKGV---------KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
             G +G  G  GD GP G  G +G++G+ G+         KG  G  GP GP G  G +G  G  GE G RG  G +GD G    PG  G PG KG  G  G+ G  GEK
Sbjct:  762 EGQKGPGGLPGDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQ---PGATGQPGPKGDVGQNGSPGPPGEK 867          

HSP 14 Score: 52.7582 bits (125), Expect = 5.306e-6
Identity = 41/92 (44.57%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 1862 AKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            A GD GP G  G +G  G  G  G  G +GP GP G  G+ G  G QG  GP G+ G+ G+ G+ GP G  G PG  G +GP G  G  GE+
Sbjct:  983 APGDPGPIGLKGDKGPPGPVGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGTPGPTGKDGIPGPPGLQGPSGAAGPSGEE 1074          
BLAST of Collagen type IV alpha 2 chain vs. UniProt/SwissProt
Match: sp|C7DZK3|CRA1A_DANRE (Collagen alpha-1(XXVII) chain A OS=Danio rerio OX=7955 GN=col27a1a PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 8.335e-33
Identity = 288/905 (31.82%), Postives = 386/905 (42.65%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLG------PRGQSGNPGPQGIRGARGQSGPTGELG------------SKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEG---KTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIG---EAGPPGKTGASGAEGQPGVKGTKGDTGILG------------------PRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNS----GPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRG----PNGP--IGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGS------PGTIGAVGSKGEPG---PPGLTGPQGKRGNPGP---KGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYK---RSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            G RGP G+PG+ G +GK+G  GP G +G        G+SG  GP+G  G +G SG TG+ G            + G +G  GP+GP G  G+                     +G PG+ G KG +G  G  G  G  G RG  G +G  GP GPDG KG+ G +G  G +G RG+ GIKG +G     GL G RGP+G SG+ G  G  G+                                 KG KG+IG +G TG+ G+IG     GP G  G  G  G PG  G +GD GI G                   +GEQG DG +          G                 G QGV+G  G  G PG  G+ GP G  GP G+ G  G+ G  G  G  G  G  G VG PG  G  G +G  G  GT G AG+ G RG PG  G+ G+ G    P  P  +G++G  GP+G  GP G  G  G+PGP G RG  G +G PG +G+R  KG  G       PG +G  G KG PG   P G+ GP+G +GN G     G  G++GPIG  G  G++G  G LG  GP G PG RG PG  G  G          + +  P    +   K   R       +D    +   +D +++ +  ++ P G  +D  AR C D+   R    DG Y+IDPN G   DSIEV C FS   +TC+  +                     + +LE ++ R            QMNFL   ++ + Q I   C    I+  +     N+      +  H +       GE L P+       V +D   +   N   ++   ++R             D ++LP++    N       S   L V  +CF
Sbjct:  995 GPRGPDGVPGEIGTEGKKGPDGPPGKIGFPGHAGKIGESGEVGPKGFPGIQGPSGATGDKGIAGEPGPSGPPGTLGPQGNPGPKGPAGKVGD---------------------SGLPGEPGEKGSIGLAGNAGAAGLIGARGEPGLEGEAGPAGPDGTKGEKGDMGTEGEQGVRGDPGIKGKDGPPGDPGLTGVRGPEGKSGKSGERGKPGL---------------------------------KGAKGNIGHLGETGSVGKIGPIGTTGPKGSRGTIGHAGAPGRMGLQGDPGISGYEGHKGPQGPIGPPGPKGAKGEQGDDGKVEGPTGAPGLRGPVGKRGDRGEPGDPGYVGQQGVDGLRGKPGAPGLPGDPGPRGTQGPKGSKGEQGQKGKQGQQGERGSRGSPGVVGLPGPRGTVGREGREGFPGTDGLAGKDGSRGTPGDQGDDGEFGLPGKPGAPGKVGVIGLPGPQGSFGPKGERGLPGHPGPSGKRGFKGGMGLPGPQGDRGSKGQPGDIGEPGFPGMLGMFGPKGPPGDFGPKGIQGPKGPQGNMGRGGLAGPVGVIGPIGNPGSRGDTGNKGELGVQGPRGAPGPRGPPGLPGPPGIPLAMNQDFGLGVVQPVFRETHTQKIEGRGLLDMPMLDQAPEILRTLDYLSSLVHSLKNPLG-TRDHPARLCRDLHDCRDTLYDGTYWIDPNLGCSSDSIEVMCNFSSGGRTCLRPI--------------------TTAKLEFSVGR-----------VQMNFLHLLSAGAEQRITIHCLNVTIWSHA----PNQPPSQNAVQFHSW------IGEVLEPD-------VLEDTCWQ--LNGRWQHADFLFR-----------VLDPALLPVVRIS-NLPKVMPSSRFHLEVGPVCF 1782          

HSP 2 Score: 88.1965 bits (217), Expect = 7.268e-16
Identity = 219/579 (37.82%), Postives = 268/579 (46.29%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGD---LGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGP---DGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPG---SPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEA------------GPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGE------------------------LGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA---------------GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +GP G  G  G PG+ G+ G  G +GPKG    +G  G  G PG  G RG+ G  G  G++G  G  G FG +GP G  GE                                    PG  G  G  GV GL+G  G  G +G    +G KG  G +G++GLKG +G+VG+ G +G+ G  G +G QG+ G PG    PG  G  GE G  G  G  GP G  G  G PG  GP G KG  G  GL GP GA GR G+             G  G  G  GA G  GVKG +G TG  GP GE+GL G +GP G  GL G +G  G  G  G  G +G  G                         +GI+GP GR+G  GP G+PG +GT G  G DG PG  G  G AG  G  G  G  G  G+QGP+G +G +G A               G  G +GP G  G+ G  G  G  G +G  G  G  G IG  G  G  G  G  G DG  GE G  G    +G++G PG  G  G  G+PG  G+ GP+GK G  G +G+ GL G  G +G  GE
Sbjct:  655 QGPVGEAGAKGYPGRQGLPGPIGPVGPKGARGFIGIPGLFGLPGADGERGSPGPPGKRGKMGRPGFPGDFGERGPPGLDGE------------------------------------PGVIGAPGPPGVLGLIGDMGPAGTVGVPGLNGLKGVPGNMGESGLKGDKGDVGLPGEQGEIGFQGDKGVQGLPGLPGPRGKPGPQGKTGEIGPSGLPGPPGPEGFPGDIGKPGLNGPEGPKGKPGARGLPGPRGAAGREGDEGPLGPPGPFGLEGQMGSKGFPGALGLEGVKGEQGVTGKAGPMGERGLVGFIGPGGEAGLAGEKGDRGEMGLPGPPGEKGSTGHPGTPGEGGPPGPPGSPGSPGSRGPIGIRGPKGRRGPRGPDGVPGEIGTEGKKGPDGPPGKIGFPGHAGKIGESGEVGPKGFPGIQGPSGATGDKGIAGEPGPSGPPGTLGPQGNPGPKGPAGKVGDSGLPGEPGEKGSIGLAGNAGAAGLIGARGEPGLEGEAGPAGPDGTKGEKGDMGTEGEQGVRGDPGIKGKDGPPGDPGLTGVRGPEGKSGKSGERGKPGLKGAKGNIGHLGE 1197          

HSP 3 Score: 70.8626 bits (172), Expect = 1.174e-10
Identity = 210/550 (38.18%), Postives = 253/550 (46.00%), Query Frame = 2
Query: 2525 GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDG------------EKGDVGPLGKTGLKGTRGEVGIKG---------------SEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIG---------------PTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE------------RGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            G+ G  G  G++G +G  G +G +G  GP G RG  G  GP GE G  G++G  G  G  G  GE+G  G  G PG  GP GP G  GSPG +G  G+ G  G +GP G  GV G +G +G  GP GP G            E G+VGP G  G++G  G  G KG                +G  G  G  G  G SG PG PG+ G  G  G  G  G  G  G  G++G  G  GP G KG KGD+G  G               P G PG  G  GP GK+G SG  G+PG+KG KG+ G LG  G  G                     + G  G +GP+G  G +G  G PGR G  G  G+ G  G  GP G  G PGP G  G+ GDDG V  P   G  GL+GP G  G +GE G+            RG  G PG+ G+ G RG  GP G           G  G  G  G  G +GSRG  G VG PG +G            T+G  G +G PG  GL G  G RG PG +G+ G  G
Sbjct:  890 GQMGSKGFPGALGLEGVKGEQGVTGKAGPMGERGLVGFIGPGGEAGLAGEKGDRGEMGLPGPPGEKGSTGHPGTPGEGGPPGPPGSPGSPGSRGPIGIRGPKGRRGPRGPDGVPGEIGTEGKKGPDGPPGKIGFPGHAGKIGESGEVGPKGFPGIQGPSGATGDKGIAGEPGPSGPPGTLGPQGNPGPKGPAGKVGDSGLPGEPGEKGSIGLAGNAGAAGLIGARGEPGLEGEAGPAGPDGTKGEKGDMGTEGEQGVRGDPGIKGKDGPPGDPGLTGVRGPEGKSGKSGERGKPGLKGAKGNIGHLGETGSVG---------------------KIGPIGTTGPKGSRGTIGHAGAPGRMGLQGDPGISGYEGHKGPQGPIGPPGPKGAKGEQGDDGKVEGPT--GAPGLRGPVGKRGDRGEPGDPGYVGQQGVDGLRGKPGAPGLPGDPGPRGTQGPKG---------SKGEQGQKGKQGQQGERGSRGSPGVVGLPGPRG------------TVGREGREGFPGTDGLAGKDGSRGTPGDQGDDGEFG 1395          

HSP 4 Score: 65.0846 bits (157), Expect = 6.417e-9
Identity = 179/446 (40.13%), Postives = 215/446 (48.21%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKG---------------DVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGEL---------------------GIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDR---------GPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGP------PGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            KG +G +G  G VG +G +G KG               + GP+G+ G KG  G  G+ G  G  G  G+RG  G+ G  G PG  G  G  G  GK+G  G  G  G  G  G PG  GE GV   IG  GP G  G IG+ GP G  G  G  G  GV G  G++G+ G +G+ GL G  G IG +G KG +G PG  G  G  GPQG  GE+                     G+ GP G KG+ G  GLPGP G    +G++G  GP G  G  G +G +GS G PG  GL+G  G  G  G+AG  G+R         G  G+AG  G RG  G  G  G  G  G PG  G  GP G PG  GS G  G +G  G KG R  +G  G PG IG  G KG  GP      PG  G  G+ G  GPKG  G+ GP G  G+ G
Sbjct:  616 KGDKGDVGLPGPVGLVGVEGRKGQKGHPGPPGLPGEPGEQGPVGEAGAKGYPGRQGLPGPIGPVGPKGARGFIGIPGLFGLPGADGERGSPGPPGKRGKMGRPGFPGDFGERGPPGLDGEPGV---IGAPGPPGVLGLIGDMGPAGTVGVPGLNGLKGVPGNMGESGLKGDKGDVGLPGEQGEIGFQGDKGVQGLPGLPGPRGKPGPQGKTGEIGPSGLPGPPGPEGFPGDIGKPGLNGPEGPKGKPGARGLPGPRGA---AGREGDEGPLGPPGPFGLEGQMGSKGFPGALGLEGVKGEQGVTGKAGPMGERGLVGFIGPGGEAGLAGEKGDRGEMGLPGPPGEKGSTGHPGTPGEGGPPGPPGSPGSPGSRGPIGIRGPKGRRGPRGPDGVPGEIGTEGKKGPDGPPGKIGFPGHAGKIGESGEVGPKGFPGIQGPSGATGDKG 1055          

HSP 5 Score: 56.225 bits (134), Expect = 3.266e-6
Identity = 94/302 (31.13%), Postives = 116/302 (38.41%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPT------------GELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPT------GAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            RGP G RG  G PG PG  G++G  G +G  G  G  G+PGP+G +G +G  G              G  GS G  G  GP+G  G +G  G                                            G  GL G+ GS G  G  G+ G+ G  GK G  G  G +G+ G +      GS GP+G  G PG PG +G                                 ++G KG +GL GP       G PG IGE G PG  G  G +G PG  G KG  G  GP+G  G
Sbjct: 1277 RGPVGKRGDRGEPGDPGYVGQQGVDGLRGKPGAPGLPGDPGPRGTQGPKGSKGEQGQKGKQGQQGERGSRGSPGVVGLPGPRGTVGREGREGF------------------------------------------PGTDGLAGKDGSRGTPGDQGDDGEFGLPGKPGAPGKVGVIGLPGPQ------GSFGPKGERGLPGHPGPSG---------------------------------KRGFKGGMGLPGPQGDRGSKGQPGDIGEPGFPGMLGMFGPKGPPGDFGPKGIQGPKGPQGNMG 1497          

HSP 6 Score: 55.0694 bits (131), Expect = 6.854e-6
Identity = 143/380 (37.63%), Postives = 172/380 (45.26%), Query Frame = 2
Query: 2972 VGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEK------GVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPG---------------TIGAVGSKGEPGPPGL---TGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
             G+KG +G  GL   + P+G  G+ G PG  G+ G +G +G++G  GP G  G  G  G  G  G K      G+ G IG +GP GA G IG  G  G  GA G  G PG  G +G  G  G  G+ G                             GP G++GE G+ G PG  G LG IG  GP GT                G  G +G  G PG  G++GL+G  G  G  GE GE G +G  G+ G  G  GP G  G  G  G  G  G  GP GP G PG  G  GL+G  G  G+ G R L G +G+ G                 G +GSKG PG  GL    G QG  G  GP GE GL+G IGP GE G
Sbjct:  598 TGLKGKKGDPGLSPGKAPKGDKGDVGLPGPVGLVGVEGRKGQKGHPGPPGLPGEPGEQGPVGEAGAKGYPGRQGLPGPIGPVGPKGARGFIGIPGLFGLPGADGERGSPGPPGKRGKMGRPGFPGDFG---------------------------ERGPPGLDGEPGVIGAPGPPGVLGLIGDMGPAGT---------------VGVPGLNGLKGVPGNMGESGLKGDKGDVGLPGEQGEIGFQGDKGVQGLPGLPGPRGKPGPQGKTGEIGPSGLPGPPGPEGFPGDIGKPGLNGPEGPKGKPGARGLPGPRGAAGREGDEGPLGPPGPFGLEGQMGSKGFPGALGLEGVKGEQGVTGKAGPMGERGLVGFIGPGGEAG 935          
BLAST of Collagen type IV alpha 2 chain vs. UniProt/SwissProt
Match: sp|Q17RW2|COOA1_HUMAN (Collagen alpha-1(XXIV) chain OS=Homo sapiens OX=9606 GN=COL24A1 PE=1 SV=2)

HSP 1 Score: 143.665 bits (361), Expect = 8.641e-33
Identity = 242/758 (31.93%), Postives = 327/758 (43.14%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDL---GPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLG------RKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLM---------GSRGPQGVSGEPGSPGDAGVN-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQ--SFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELS 4696
            +G PG+ G  GLPG+PG+ G +G +GP G++   GP G  G  G QG  GA+G  G  G +G  G+ G  G  G  G++G +G  G +G PG  G  G  G  G  G  G  G +G  G  G  G +G+ G+ G      +KG  G IGP GE G  GP GK G  G +G  G +G+ G  GLM         G RG QG  G  G PG  G       +    G  GP G  G  G  G+ G  G +G KG +G+ G  GA G+ G  G PG  G  G +G+ G +G KG  G  GP G+ G+ G  G +G KG++G  G  G  G+ G  GP G +G  GI+G PGR G  G  G PG  G                           S GLPG  G+QGP          GE+G  G PGI G  G RG  G  G                             G  G  G+PG+ G + L G +G PG  G  G  G  G  G  GP G RGN GP G  G++GP G  G  GE                                R        I+   E ++     S +    + +D ++ +F  ++ ++N +  I+ P G  +D+ AR C D+    ++ +DG+Y+IDPN G   D+IEV C FS   +TC+  V                    +  +LE  + +            QMNFL   +S++   I   C  +  +  ++ S
Sbjct:  977 QGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSRGPPGKIGKSGPKGARGTRGAVGHLGLMGPDGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPGEDSTVLGPPGPRGEPGPVGDQGERGEPGAEGYKGHVGVPGLRGATGQQGPPGEPGDQGEQGLKGERGSEGNKGKKGAPGPSGKPGIPGLQGLLGPKGIQGYHGADGISGNPGKIGPPGKQGLPGIRGGPGRTGLAGAPGPPGVKG---------------------------SSGLPGSPGIQGP---------KGEQGLPGQPGIQGKRGHRGAQGDQGPC---------------------------GDPGLKGQPGEYGVQGLTGFQGFPGPKGPEGDAGIVGISGPKGPIGHRGNTGPLGREGIIGPTGRTGPRGE-----KGFRGETGPQGPRGQPGPPGPPGAPGPRKQMDINAAIQALIESNTALQMESYQNTEVTLIDHSEEIFKTLNYLSNLLHSIKNPLG-TRDNPARICKDLLNCEQKVSDGKYWIDPNLGCPSDAIEVFCNFSAGGQTCLPPV--------------------SVTKLEFGVGK-----------VQMNFLHLLSSEATHIITIHCLNTPRWTSTQTS 1634          

HSP 2 Score: 115.546 bits (288), Expect = 2.901e-24
Identity = 204/523 (39.01%), Postives = 250/523 (47.80%), Query Frame = 2
Query: 2537 IDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXX----------------XXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNP------GPKGETGLMGPIGPVGEDG 4039
            + G  G+ GP G  GP+GQ G+ GP G+ G +G  G  GE G +G RG  G  G TG+ GERG  G  G  G  G  G                           ++G+ G  G +G  G +GP GE G  GP G  G  G +GE G KG  G  G +G  G  G+ GEPG+PG+ G+                G  G++G+PG  G  GE G KG++GL G  G  GR G+ G PG  G  G  GQ G  G KGD G +GP GE G     GP G  G  G +G  G +G+ G+ G  G +GE GI G  G +G+ GP GLPGP G  G  G+D     P G                    G  G VG PGL G  G QGP G  G QGE G +G+RG  G  G  G  GP+G  GI G  G  G  G  G  G  G  G  G  G  GK G PG +G     G+ G+PG  G  GS G PG PG+ GP+G++G P      G +G  G  G  GP G+ G
Sbjct:  907 LPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTG---------------------------DRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQ---------------GKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVG---SRGPPGKIGKSGPKGARGTRGAVGHLGLMGPDGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPGEDSTVLGPPGPRGEPGPVGDQGERGEPGAEGYKGHVGVPGLRGATGQQGPPGEPGDQGEQGLKGERGSEGNKGKKGAPGPSGKPGIPGLQGLLGPKGIQGYHGADGISGNPGKIGPPGKQGLPGIRGGPGRTGLAGAPGPPGVKGSSGLPGSPGIQGPKGEQGLPGQPGIQGKRGHRGAQGDQGPCGDPG 1384          

HSP 3 Score: 110.923 bits (276), Expect = 7.170e-23
Identity = 175/514 (34.05%), Postives = 214/514 (41.63%), Query Frame = 2
Query: 2534 GIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKG---SVGPIGPDGEKGDVGPLGKTG---------LKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPG------RIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            G  G+EG IG  G+LGPRG+   PG +G  G  G  G  GE+G +G+ GK G  GP G  G                                             E G+ G +G KG   S GP+GP GEKG +G  G  G         L G  G  G  GS+G  G  GSRGP G+ GE G  G  G                               +G++G +G  GLIG TG PG      + G  G PG TG  G  G+PG++G +GD   +GP GE G++                           GP G EGE G+QG PG KG++G  G  G  G  G  G+ GAPG                 GL GK GL+G  GG G  GE GE+G+ G PGI G +G+ G  G                                G +G VG PGQ+G    KG KG  G  G VGS+G PG  G +GP+G RG  G  G  GLMGP G
Sbjct:  804 GTQGEEGPIGAFGELGPRGK---PGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPG---------------------------------------------EVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKG------------------------------EKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGD---VGPPGEMGME---------------------------GPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEE---------------GLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTG------------------------------LPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSRGPPGKIGKSGPKGARGTRGAVGHLGLMGPDG 1164          

HSP 4 Score: 109.768 bits (273), Expect = 1.892e-22
Identity = 227/577 (39.34%), Postives = 274/577 (47.49%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---------------PGPQGIRGARGQSGPTGELGSK---------------GDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDV------GPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGS------------------PGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEK---GVKGDIGLIGPTGAP------GRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNM---GQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGK---RGNPGPKGETGLMG 4012
            GP G  GP+G+PG  G+ G +G  G KGD G +G +G                PGP G+RG  G SG TG+ G +               G  G+ GP+GP G  G RG PG  G  G  G  GPIG  G                     E G RG  G+KG  G  GP+G KG+V      G +G+TG  G  GEVG+ GS G+ G  GS GP G  GE G                   PG  G  G  G QG +G +G  GP G+ G  G  G +GEK   G +G  GLIG TG P      G+ G  G PG TG  G  G+PG++G +GD   +GP GE G++                           GP G EGE G+QG PG KG++G  G  G  G  G  G+ GAPG                 GL GK GL+G  GG G  GE GE+G+ G PGI G +   GQ G  GP GIVG  G RG+PG  G  G IG  G  GSRG  GK+G+ G KG R   G +G+ G +G +G  GEPG PG  G QG+    G PGPKGE G  G
Sbjct:  687 GPVGPIGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYPGDKGAVGLPGPPGMRGKSGPSGQTGDPGLQGPSGPPGPEGFPGDIGIPGQNGPEGPKGLLGNRGPPGPPGLKGTQGEEGPIGAFG---------------------ELGPRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGD---VGPPGEMGME---------------------------GPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEE---------------GLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSRGPPGKIGKSGPKGAR---GTRGAVGHLGLMGPDGEPGIPGYRGHQGQPGPSGLPGPKGEKGYPG 1194          

HSP 5 Score: 100.523 bits (249), Expect = 1.193e-19
Identity = 202/550 (36.73%), Postives = 253/550 (46.00%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGE---LGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGE---------------KGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGE---PGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXX------------XXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GPPG +G  GL G PG+ G  G  G  G  G  G  G PG QG+ G  G  GP G    +GS G+ G+ GP+G  G  G RG  G                                        KG +G  G  G  GP G DG                +G VGP+G  G  G  G +G+ G++G  G+ G +G QG +GE   PG PGD G  G  G  G +G  GP+G  G  G+ G  GP G +G  GDIG+      PG+ G  GP G  G  G  G PG+KGT+G+ G +G  GE G                RGKPG++G  G  GP+G++GE+G QG  G+ GE GP+GLPG VG +G  G+ G  G              G  G  G  GP+G  GLPG  G +GP G   +QG  G+RG RGP G+ G  G +G  G  G                         +G RG  G +G+ G  GER  +G  G  G  G+ G +G PG PGL G Q   G+ GP GE G+ GP G  GE G
Sbjct:  561 GPPGMQGDKGLKGHPGLPGLPGEQGIPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFIGSPGEAGQLGPEGERGIPGIRGKKGF---------------------------------------KGRQGFPGDFGDRGPAGLDGSPGLVGGTGPPGFPGLRGSVGPVGPIGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYPGDKGAVGLPGPPGMRGKSGPSGQTGDPGLQGPSGPPGPEGFPGDIGI------PGQNGPEGPKGLLGNRGPPGPPGLKGTQGEEGPIGAFGELG---------------PRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPG---SQGPKGQRGSRGPDGLLGEQGIQGAKGEKG------------------------DQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQ---GDVGPPGEMGMEGPPGTEGESG 1020          

HSP 6 Score: 81.2629 bits (199), Expect = 8.494e-14
Identity = 172/521 (33.01%), Postives = 216/521 (41.46%), Query Frame = 2
Query: 2504 RGPSGLP---GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGP G+P   G PG+ G  G  GPKGD G       PG +G +G  G  GP G  G KG +G  G  G  G++G                                 + G  G  G  G  G +GL G +G+ GP      IG  GE G +GP G+ G+ G RG+ G KG +G  G  G RGP G+ G PG                              + G  GP G  G++G +G +GP      IG AG PG  G SG +G PG+KG KG+ G  G  GE G                                G +G +G+ GPPG +G+ GP G  G             PG  G SG  G +G  G  G+PG+ G +GP      +G  G RG  GPPG+ G  G+ GP G  G +                           G  GK G+ G  GE   +G+KG  G  G +G  GE GP GL G  G  G+ G KGE G  GP+GP GE G
Sbjct:  512 RGPRGIPGPHGNPGLPGLPGPKGPKGDPGFSPGQPVPGEKGDQGLSGLMGPPGMQGDKGLKGHPGLPGLPGEQG---------------------------------IPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFIGSPGEAGQLGPEGERGIPGIRGKKGFKGRQGFPGDFGDRGPAGLDGSPG------------------------------LVGGTGPPGFPGLRGSVGPVGP------IGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYP------------------------------GDKGAVGLPGPPGMRGKSGPSGQTG------------DPGLQGPSGPPGPEGFPGDIGIPGQNGPEGP------KGLLGNRGPPGPPGLKGTQGEEGPIGAFGEL---------------------------GPRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKG 888          

HSP 7 Score: 70.4774 bits (171), Expect = 1.835e-10
Identity = 190/552 (34.42%), Postives = 235/552 (42.57%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP------IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGG------------SGTQGEAG------ERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGI------KGSPGTIGAV---GSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RGP G  GP G PG PG+ G +G   PKGD G       PG +G +G  G  GP G  G KG +G  G  G  G++G                                 + G  G  G  G  G +GL G +G+ GP      IG  GE G +GP G+ G+ G RG+ G KG +G  G  G RGP G+ G PG                              + G  GP G  G++G +G +GP      IG AG PG  G SG +G PG+KG KG+ G  G  GE G                                G +G +G+ GPPG +G+ GP G  G             PG  G SG  G +G  G  G+PG+ G +GP G              GTQGE G      E G RG PG  G  G+ GP G  G VG  G  G+ G  GP+G  G  G  GS G  G+ G PG  G +  KG+       G PG IG +   G  G  GPPG  GP+G+RG+ GP G  G  G  G  GE G+
Sbjct:  512 RGPRGIPGPHGNPGLPGLPGPKG---PKGDPGFSPGQPVPGEKGDQGLSGLMGPPGMQGDKGLKGHPGLPGLPGEQG---------------------------------IPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFIGSPGEAGQLGPEGERGIPGIRGKKGFKGRQGFPGDFGDRGPAGLDGSPG------------------------------LVGGTGPPGFPGLRGSVGPVGP------IGPAGIPGPMGLSGNKGLPGIKGDKGEQGTAGELGEPGYP------------------------------GDKGAVGLPGPPGMRGKSGPSGQTG------------DPGLQGPSGPPGPEGFPGDIGIPGQNGPEGPKGLLGNRGPPGPPGLKGTQGEEGPIGAFGELGPRGKPGQKGYAGEPGPEGLKGEVGDQGNIGKIGETGPVGLPGEVGMTGSIGEKGERGSPGPLGPQGEKGVMGYPGPPGVPGPIGPLGLPGHVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGD 949          

HSP 8 Score: 63.5438 bits (153), Expect = 1.826e-8
Identity = 48/108 (44.44%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGV------KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            YKG  G+ G  GA G  GP G+ G +GE G+KG       KG+ G  GP G  GI G++G +G +G  G  G DG  G+ G +GPPG  G PGI+G  G  G  G  G
Sbjct: 1226 YKGHVGVPGLRGATGQQGPPGEPGDQGEQGLKGERGSEGNKGKKGAPGPSGKPGIPGLQGLLGPKGIQGYHGADGISGNPGKIGPPGKQGLPGIRGGPGRTGLAGAPG 1333          

HSP 9 Score: 62.3882 bits (150), Expect = 4.277e-8
Identity = 46/94 (48.94%), Postives = 55/94 (58.51%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQG---PRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            G +G RG  GA+GD GP G  G +G+ G  GV+G  G QG   P+GPEG AG+ G  G +G  G RG  G  G EG +GP G  G  G KG RG
Sbjct: 1363 GIQGKRGHRGAQGDQGPCGDPGLKGQPGEYGVQGLTGFQGFPGPKGPEGDAGIVGISGPKGPIGHRGNTGPLGREGIIGPTGRTGPRGEKGFRG 1456          

HSP 10 Score: 59.6918 bits (143), Expect = 3.397e-7
Identity = 48/102 (47.06%), Postives = 58/102 (56.86%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            + GARG  GS G KG  G  G  G  GE G++G KGE G QG RGP G+ G  G+    GE G +GK G +G  GS G  G  G PG++G +G VG  G  G
Sbjct:  910 HVGARGPPGSQGPKGQRGSRGPDGLLGEQGIQGAKGEKGDQGKRGPHGLIGKTGN---PGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMG 1008          

HSP 11 Score: 56.9954 bits (136), Expect = 1.894e-6
Identity = 47/118 (39.83%), Postives = 64/118 (54.24%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV------------KGDIGIQGETGPRGKDGNK---GDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G  G++G  GAKGD G +G VG  GE G++G  G  G +G +G +G+ GV            KG++G+ G  GP G+ G     G EG VG PG  G PG KG +G +G  G  G +
Sbjct: 1016 EGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQGKDGLKGVPGGRGLPGEDGEKGEMGLPGIIGPLGRSGQTGLPGPEGIVGIPGQRGRPGKKGDKGQIGPTGEVGSR 1133          

HSP 12 Score: 56.6102 bits (135), Expect = 2.698e-6
Identity = 43/102 (42.16%), Postives = 56/102 (54.90%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G RG +G  G +G  G +G  G  GE G++G++G+ G  G  G EG  G +G+ G+QGE G +G  G  G  G  G PG  G PG  G  G    QG+DG K
Sbjct:  966 GERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEG---LQGKDGLK 1064          

HSP 13 Score: 55.8398 bits (133), Expect = 4.866e-6
Identity = 42/102 (41.18%), Postives = 56/102 (54.90%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +GA+G +G  G +G HG  G+ G  GE G +G  G  G+ G  G  G+ G  G  G+QG+ GP G+ G +G  G+ G  G  G PG KG  G  G+ G  GE
Sbjct:  941 QGAKGEKGDQGKRGPHGLIGKTGNPGERGFQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGE 1042          

HSP 14 Score: 55.4546 bits (132), Expect = 6.534e-6
Identity = 45/110 (40.91%), Postives = 61/110 (55.45%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGV------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            ++G  G++G  G+ GD G  G+ G RG  G  G  GE G++GP G EG +G+      KGD+G  G  G  G+ G +G+ G+ G  G  G  G+KG  G  G  G DGEK
Sbjct:  970 FQGKPGLQGLPGSTGDRGLPGEPGLRGLQGDVGPPGEMGMEGPPGTEGESGLQGEPGAKGDVGTAGSVGGTGEPGLRGEPGAPGEEGLQGKDGLKGVPGGRGLPGEDGEK 1079          

HSP 15 Score: 55.0694 bits (131), Expect = 7.476e-6
Identity = 113/275 (41.09%), Postives = 131/275 (47.64%), Query Frame = 2
Query: 3215 PGRIGEAGPPGKTGASGAEGQPGVKGTKGDTG------ILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPP------GLTGPQGKRGNPGPKGETG 4003
             G+ G  G PG  G  G  G PG KG KGD G      + G +G+QGL G +GP G +G KG +G PG  G  G  G  G  G +G  G PGR+G  GP G PGP G  G                          G PG+AG  GP G  G  G  G++G +G  G  G+ G RGP G  G  G  G  G PG  G  G +G  GP G  G+ G +G  G KG   L GIKG  G  G  G  GEPG P      GL GP G RG  GP G+TG
Sbjct:  509 SGKRGPRGIPGPHGNPGLPGLPGPKGPKGDPGFSPGQPVPGEKGDQGLSGLMGPPGMQGDKGLKGHPGLPGLPGEQGIPGFAGNIGSPGYPGRQGLAGPEGNPGPKGAQGFI------------------------GSPGEAGQLGPEGERGIPGIRGKKGFKGRQGFPGDFGDRGPAGLDGSPGLVGGTGPPGFPGLRGSVGPVGPIGPAGIPGPMGLSGNKG---LPGIKGDKGEQGTAGELGEPGYPGDKGAVGLPGPPGMRGKSGPSGQTG 756          
BLAST of Collagen type IV alpha 2 chain vs. UniProt/SwissProt
Match: sp|Q5QNQ9|CORA1_MOUSE (Collagen alpha-1(XXVII) chain OS=Mus musculus OX=10090 GN=Col27a1 PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.158e-31
Identity = 286/771 (37.09%), Postives = 369/771 (47.86%), Query Frame = 2
Query: 2543 GKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKG------------DVGPLGKTGLKGTRG------EVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXX-NSGPQGVEGELGIQGPPGRKGELG---PIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGT------------QGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG---------PVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIY----GDSELSEENKFK 4714
            G  G  G KG  GPRG  G  G QG +G +G+ GP G  G  G +G  G +G +G KG  G PG  GPPG  G  G  G  G  G  G  G +G  GP G +GE G+ G  G KG +GP+GP GE+G            D GP+G  GLKG RG      E G KG EG  G  GS GP G++G PG  G  G  GEKG++G +G++G  G +G  GIPG+PGP G  G KG  G +GPTGAPGR+G  G PG  G +G +G   + G  G  G  G +G+QG DG            G            + G  G EG  G++G PG++G+ G   P G PGP G+ G  G  G PG  G SG+ G  G  G PG  G  G QGP G +G+            QG+ G  GD GP G AG  G  G  G  G  GP G +G+ G  G +GP G  G +G  GL G  GEPG KG+    G  G PG  G  G KG PG  G  G QG RG PG  G+ G++GP G         P G+ G  G  G  GP GPPGP G  G PG         +       +     +G+      S   Q  +D    +F  +  ++N I+ I+ P G  K++ AR C D+    ++  DG Y++DPN G   D+IEV C F++  +TC+  +                     + + E A+SR            QMNFL   +S+  Q I   C    ++    G S   +  +F+
Sbjct: 1039 GLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKG---ITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDLGLQGPRGPPGPRGRPGPPGPPWHPIQFQQDDLGAAFQTWMDAQGAVRSEGYSYPDQLALDQGGEIFKTLHYLSNLIQSIKTPLG-TKENPARVCRDLMDCEQRMADGTYWVDPNLGCSSDTIEVSCNFTQGGQTCLKPI--------------------TASKAEFAVSR-----------VQMNFLHLLSSEGTQHITIHCLNMTVWQEGPGRSSARQAVRFR 1774          

HSP 2 Score: 91.6633 bits (226), Expect = 5.333e-17
Identity = 229/579 (39.55%), Postives = 279/579 (48.19%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDG---KEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGEL------------GSKGDRG------------KFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK------------GDVGPLGKTGLKGTR------GEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLD-XXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGE---LGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGT------------QGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RG PG RG  G  G  G DG   ++GS G KG +GPRG+ G PG QG  G RG SG  G L            G  G+ G            + GP+GP G  GE                                              G+ G  G KG +GP+GP GE+            GD GP+G  GLKG R      GE G KG EG  G  GS GP G++G PG  G  G  GEKG++G +G++G  G +G  GIPG+PGP G  G KG  G +GPTGAPGR+G  G PG  G +G +   G+ G  G  G  G +G+QG D    GP G  G +G  G  G +G  G+ G  G EG  G++G PG++G+    GP G PGP G+ G  G  G PG  G SG+ G  G  G PG  G  G QGP G +G+            QG+ G  GD GP G AG  G  G  G  G  GP G +G+ G  G +GP G  G +G  GL G  GEPG KG+    G  G PG  G  G KG PG  G  G QG RG PG  G+ G++GP G +G  G
Sbjct: 1038 RGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGE---------------------------------------------PGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHK---GITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSG 1568          

HSP 3 Score: 80.1073 bits (196), Expect = 2.184e-13
Identity = 201/620 (32.42%), Postives = 245/620 (39.52%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPG---IDGKEGSIGPKGDL------------GPRGQSGNPGPQGIRGARGQSG------PTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGE---------KGDVGPLGKTGLKGTRGEVGIKGSEGKTGL---------------------------------MGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXS---PGLPGKAGLQGPAGGSGTQGEAG-------------------------------------ERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GPPG  GP G+ GKPG   + G  G +GP+GD             G  G+ G PG  G+ G++G+ G      P GE G  G  G  G  G  G+KG+RG  G  G PGP G  G  G  G  G+ G  G  G PG       +G+ G+ G KG  GP GPDG          KG VGP G+ G  G +G  G +G  G  G                                  MG +GP G  GEPG PGD+                                    G+KGD+G +GP G  G IG+ G PG  G  G  G  G+KG +GD G  G  GE+G +                  G +G  G+ GP G+ G  G +G PG++GE G  G  G  G  G  G+ G PG PG  G  G  G  G+    G PG+ G QG  G +G  G  G                                     +RGDRG PG  G  GQ G  G                               RG  G+ G+PG  G R   G KGS G  G  G  G+ GP G  G QG +G PGP+G  G  GP G  G DG
Sbjct:  901 GPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPG------SRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDS------------------------------------GMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQE------------------GLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQGYLGEMGIPGEPGPPGTPGPKGSRGTLGPTGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGDQGEDGKTEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQG------------------------------LRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDG 1430          

HSP 4 Score: 71.2478 bits (173), Expect = 9.571e-11
Identity = 114/338 (33.73%), Postives = 143/338 (42.31%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVG 3517
            RG  G PG PG  G+EG  G +G+ G +GQ G+PGP      RG+ GP G  G +G +GK G  GP+G +G                                              +G++GL G +G VG  GP+G  G  G  G+ G  G +G+ G  G  G  G  G RG  GV+G PG+ G                                      G KG+ GL G  G PG+ G     G TG  G +G+PG KG  GD+G +G  G  GL          G KG  G  G +G  G  GP G+ G+ GI GPPG    LGP GLPGP G
Sbjct: 1339 RGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGP------RGRPGPKGSKGEEGPKGKPGKAGPSGRRG---------------------------------------------TQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPP------------------------------------GFKGESGLPGQLGPPGKRGTE---GGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGL---------FGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGM---LGPSGLPGPKG 1574          

HSP 5 Score: 70.8626 bits (172), Expect = 1.142e-10
Identity = 213/568 (37.50%), Postives = 254/568 (44.72%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG---PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ------GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASG------------AEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPP---GRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDR------------GPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXX---------XXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLK---GIKGSPGTIGAVGSKGEPGPPGLTGP------QGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G  GP G PG  G  G++G  GP GD GP+G  G  G  G+ G  G  G  G  G  G RG+ G  G  GD GERG PG  G PG  G  G                             GV GL+G  G++GP+G   P G KG +G +G+ GLKG +GE G+ G  G  G  G +G        G  G+PG  G AG                 G +G  G PG  G  G+ G  GD G  G  G PG  G  GPPG+ G  G             EGQPG KG  G  G+ G +GE G                       G  G  G QG+ G +G+ G P   G KG+ G +G PG  G  G  G  G PG  G  G+ G  GP GS GLPG  G +G  G  G  G AGE+G +            G PG  G  G+RG +G  G +G  GP G PG           GP GP+G PG  G +G  G VGEPG  G+  +K   G  G PG  G +G +GEPG  G  GP      +G RG+PGP GE G  G  G  GEDG
Sbjct:  709 GQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPG---------------------------PPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAG---------------DKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPG---------------------DPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDG 1213          

HSP 6 Score: 70.8626 bits (172), Expect = 1.264e-10
Identity = 48/102 (47.06%), Postives = 63/102 (61.76%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G+ G SG KGD GP G  G +G +G +G  G  G  GP GP+G+ G +GD G  GE G +G++G KG++GS GPPG  G PG +G  G  G +G+ G K
Sbjct: 1141 GEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAK 1242          

HSP 7 Score: 68.1662 bits (165), Expect = 8.697e-10
Identity = 187/514 (36.38%), Postives = 225/514 (43.77%), Query Frame = 2
Query: 2525 GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVE------------GELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXX---------XXXXXXXXXXXXXSRGLDGKVGEPGQKGER---YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG 3994
            G PG+   +   G KG++G  G SGNPGP G +G +G  G     G  G++G+ GP+G                                                     G +G  GR+G  GP+G      D GP G  G  G  G  G+ GS+G+ GL G  G +G  G PG PGD G  G  G  G  G  G                    G  G +GLIG TGA G +G  GP G  G  G  G+PG+KG KG+ G+ G  G+ G  G  G  G  GL GGRGKPG  G  G+ G  G              G  G  GP G KG+ G  GLPGP G  GP G +G  GPPG  G  G  G  G PG PG  G +G  G  G  G  GE+G  G  G+ G  G  G  G  G++GP G  G  G +         G  G  G  GP GSRGL G  G  G +G R      G +GS G  G VG +G PG PG  G  G+RG+ G KG
Sbjct:  661 GDPGLSPGQAHDGAKGNMGLPGLSGNPGPLGRKGHKGHPG---AAGHPGEQGQPGPEG---------------------------------------------------SPGAKGYPGRQGFPGPVG------DPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGL------------------PGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKG 1096          

HSP 8 Score: 64.6994 bits (156), Expect = 9.590e-9
Identity = 49/109 (44.95%), Postives = 58/109 (53.21%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG------PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + G RG  G  G+KG+ GP G+ G  G  G +G +G  G+ GPRG      PEG AG  G  G  G  G +G  GN GD G VGP G  GNPG+ G  G  G  G  GE
Sbjct: 1371 HPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGE 1479          

HSP 9 Score: 63.1586 bits (152), Expect = 2.442e-8
Identity = 142/400 (35.50%), Postives = 160/400 (40.00%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP---GKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---PGPKGETGLMGPIGPVGEDG 4039
            G  G  G KG  G  G  GE+G  GP G  G KG  G  G  G  G  G  GSRG  G+ G  G PG  G  G                                GV G  G +G  G PG  GE GPP   G  G  G  G PGV G  GDTG LGP G                                GP+G++G +G  G PG KG+ G  G+PG  G        G  G  G  G  G  G  G PG  GKAG +G  G  G  G  G  GD GPPG  G  G +G  G                              +RGL G  G+ G +G+    G  G PG  G  G KG PG PGL G +G+ G+   PGP GE GLMG IG VGE G
Sbjct:  688 GPLGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGL------------------------------PGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVG------------------------------YPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGD------PGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPG------------------------------ARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPG 991          

HSP 10 Score: 61.2326 bits (147), Expect = 9.884e-8
Identity = 204/589 (34.63%), Postives = 251/589 (42.61%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGK------------PGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGD---------------VGPLGKTGLKGT------------RGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGAS---GAEGQPGVKGTKGDTGI------------LGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER------YLKGIKGSPGTIGAVGSKGE---------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GP G+RG  GLPG             PG+ GK G +G  G  G  G+ G PG  G  G  G  GP G LG  GD G  GP G  G KG +                                    G  G +GE G++G  G +G  G  G  G +GD                GPLGK G KG+             G++G  G  G  G+ G  G +G+ G PG  G  G                 GP+G  G+PG  G  G KG  G  GL G  G PG  G  GP G+ G     G  G+PG+ G KGD G+            +G  G  G  G  G  G  G  G RG PG +G+ G+ GP+G +G  G QG  G KG +GP G PG  G  G +G+ G  G  G  G  G  GP G+ GLPG+ G QGP G  G  GE G +G  G    PG+ G+ G +G  GP+G                        P G +GL G+ GEPG +G+        LKG +G PG  G  G KG+         PGPPG+TG  G+ G PG +GE G  G  G  G  G
Sbjct:  736 GPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMK------------------------------------GLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEG---------------DEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLG------------------------PPGEQGLIGQRGEPGLEGDHGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEDGSPGPPGITGVPGREGKPGKQGEKGQRGAKGAKGHQG 1249          

HSP 11 Score: 60.077 bits (144), Expect = 2.333e-7
Identity = 48/111 (43.24%), Postives = 57/111 (51.35%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP---------PGYAGNPGIKGSRGPVGNQGRDG 2131
            Y G  G++G  G  G  G  G  G RG  G KG KGE G +G  G  G +G +G  G+QG  GPRG  G +G EG+ G          PGY G+ G  G  GPVG  GR G
Sbjct: 1350 YPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGPSGRRGTQGLQGLPGPRGVVGRQGPEGTAGSDGIPGRDGRPGYQGDQGNDGDPGPVGPAGRRG 1460          

HSP 12 Score: 58.9214 bits (141), Expect = 5.891e-7
Identity = 194/552 (35.14%), Postives = 240/552 (43.48%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---GDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ---GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKV---GEPGQKGERYLKGIKGSPGTIGAV---------GSKGEPGPPGLT---------------GPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G PG  G  G  G+PG +G  G+ G  G  G  G  G+PGP+G RG  G  G  G  GS G+RG  G  G  G+ G                                     PG  G  GE+G  GL G  G +G  GP G     GD G LG  G  G +G  G+ G  G+ GL G +G Q   GVSG+PG  GD G +               G  G+ G  G+PGP G+ G KG +G  GP G  G  G+ GPPG  G  G +G+PG +G  G  G LGP G++         G  G  G  G  G+ G  G  G  G++G  G  G PGR G +G  GL G +G  G  G  G  G  G  G  G  GP GS G PG  G  G  G  G  G  G RG  G  G  G+ G RGP+GP G                         +GS+GL G+V   G PGQ G++   G +G  G  G +         G+KG PG PG                 GP G  G PG  G++G+ G +GP+G  GE
Sbjct:  696 KGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGEMGR------------------------------------PGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLIGDTGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSH---------------GLPGLPGGRGKPGPLGKAGDKGSLGFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDE---------GPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFIGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGIGNPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAG------------------------EQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGE 1163          

HSP 13 Score: 56.225 bits (134), Expect = 3.819e-6
Identity = 47/103 (45.63%), Postives = 54/103 (52.43%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + GA+G  G  G  G+ GP G+ G +G  G  G  GE   QG  GPEG  G KG  G QG  GP G  G KG  G +G PG  G PG  G RG  G  G+ GE
Sbjct:  671 HDGAKGNMGLPGLSGNPGPLGRKGHKGHPGAAGHPGE---QGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVPGKRGE 770          
BLAST of Collagen type IV alpha 2 chain vs. UniProt/SwissProt
Match: sp|P02465|CO1A2_BOVIN (Collagen alpha-2(I) chain OS=Bos taurus OX=9913 GN=COL1A2 PE=1 SV=2)

HSP 1 Score: 137.502 bits (345), Expect = 5.193e-31
Identity = 312/935 (33.37%), Postives = 415/935 (44.39%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGP---QGIRGARGQSGPTG---------------ELGSKGDRG---KFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLG---------KTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPG---------------SPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRI---------------GEAGPPGKTGASGAEGQP---------------------GVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGE------LGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGS---PGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFD---KVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDH--TQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRT-PGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            PG +GPSG PGK G  G  G  G +G  GP G +G  GP   QG++G +G+ GP G               E G  G+RG   +FG  GP G +GERG PG  G  GP GP G  G +G PG  G                KG  G++G  G+ GP GP G  G+ G  G         +TGL+G  G  G  G+ G  G +G+ GP G +G+ G               SPG+ G      E G  G  G  GP G  G PG  G RG KG KG+ G +GPTG  G                 G+ GPPG TG  GA G+                      G++G +GD G +G  GE G  GP G +G KG  G  G  G  G+ G  G  G  G LG+   PG +GE       G +G PGP+G +GP G  G PG  G  G  G  G  G  G PG  G  G  G  G +GE G  G+ GP G AG  G +GP GP+G  G  G  G  G +GP G +G  GP G +G+ G  GEPG KG R L G+KG     G  G  G  G+ G PG  GP G      P+G  G  GP G  G  G+ GA GP G  G  G  G  G PG  G  G  G  G   +        G  G + R+ + +S   +    ++    +  + N+I+ +  P G  K+ A RTC D++L   + + G Y+IDPN+G   D+I+V C FS   +TCI +  +   + N+ +  K + +      + G      ++  +      TQ+ F+R   + + Q I + C  S+ Y D E       K A +L                   +G  D  +  +   R  F  T+  DGC  +T     +  + +T   S LPI+D    D        + LN+  +CF
Sbjct:  493 PGPKGPSGDPGKAGEKGHAGLAGARGAPGPDGNNGAQGPPGLQGVQGGKGEQGPAGPPGFQGLPGPAGTAGEAGKPGERGIPGEFGLPGPAGARGERGPPGESGAAGPTGPIGSRGPSGPPGPDG---------------NKGEPGVVGAPGTAGPSGPSGLPGERGAAGIPGGKGEKGETGLRGDIGSPGRDGARGAPGAIGAPGPAGANGDRGEAGPAGPAGPAGPRGSPGERG------EVGPAGPNGFAGPAGAAGQPGAKGERGTKGPKGENGPVGPTGPVGAAGPSGPNGPPGPAGSRGDGGPPGATGFPGAAGRTGPPGPSGISGPPGPPGPAGKEGLRGPRGDQGPVGRSGETGASGPPGFVGEKGPSGEPGTAGPPGTPGPQGLLGAPGFLGL---PGSRGERGLPGVAGSVGEPGPLGIAGPPGARGPPGNVGNPGVNGAPGEAGRDGNPGNDGPPGRDGQPGHKGERGYPGNAGPVGAAGAPGPQGPVGPVGKHGNRGEPGPAGAVGPAGAVGPRGPSGPQGIRGDKGEPGDKGPRGLPGLKGHNGLQGLPGLAGHHGDQGAPGAVGPAG------PRGPAGPSGPAGKDGRIGQPGAVGPAGIRGSQGSQGPAGPPGPPGPPGPPGPSGGGYEF-------GFDGDFYRADQPRSPTSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPA-RTCRDLRLSHPEWSSGYYWIDPNQGCTMDAIKVYCDFSTG-ETCIRAQPEDIPVKNWYRNSKAKKHVWVGETINGGTQFEYNVEGVTTKEMATQLAFMRLLANHASQNITYHCKNSIAYMDEET---GNLKKAVIL-------------------QGSNDVELVAEGNSR--FTYTVLVDGCSKKTNEWQKTIIEYKTNKPSRLPILDIAPLDIGGA-DQEIRLNIGPVCF 1363          

HSP 2 Score: 75.0998 bits (183), Expect = 6.628e-12
Identity = 148/408 (36.27%), Postives = 172/408 (42.16%), Query Frame = 2
Query: 2864 RGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIG---LPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---------------------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGP 4015
            RGL G  G  G +GP G +G  GP G       RG  G  G  G+ GLMG   P+G  G PG+ G AG  G  G                             G+ G  G IGP GA G  G  G PG  G SG    PG  G KG  G+ G RG  G DG                        N+G QG  G  G+QG  G +G  GP G   LPGP GT+G +GK G                    G+PG+ GL GPAG  G +G  GE G  GP                     PG+ G  G  GP+GP G+         PG  G  G  G  G KG  GL G +G PG+ G R   G  G+PG  GA G +GE GP G  GP G RG+PG +GE G  GP
Sbjct:  403 RGLPGADGRAGVMGPAGSRGATGPAG------VRGPNGDSGRPGEPGLMG---PRGFPGSPGNIGPAGKEGPVGL---------------------------PGIDGRPGPIGPAGARGEPGNIGFPGPKGPSG---DPGKAGEKGHAGLAGARGAPGPDG------------------------NNGAQGPPGLQGVQGGKGEQGPAGPPGFQGLPGPAGTAGEAGKPGER------------------GIPGEFGLPGPAGARGERGPPGESGAAGPTGPIGSRGPSGPPGPDGNKGEPGVVGAPGTAGPSGPSGL---------PGERGAAGIPGGKGEKGETGLRGDIGSPGRDGARGAPGAIGAPGPAGANGDRGEAGPAGPAGPAGPRGSPGERGEVGPAGP 720          

HSP 3 Score: 72.7886 bits (177), Expect = 3.003e-11
Identity = 152/421 (36.10%), Postives = 174/421 (41.33%), Query Frame = 2
Query: 2939 GKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPI---GLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---------------------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE---------------PGPPGLTGP------------------QGKRGNPGPKGETGLMGPIGPVG 4030
            G  G  G  G +G  GS G TG  G RGP G SG PG PG  G  G  G  G  G  G  GPVG+             G+ G  G IGP GA G  G  G PG  G S   G PG  G KG  G+ G RG  G D                        GN+G QG  G  G+QG  G +G  GP    GLPGP GT+G +GK G                    G+PG+ GL GPAG  G +G  GE G  GP                     PG+ G  G  GP+GP G+         PG  G  G  G  G KG  GL G +G PG+ G R   G  G+PG  GA G +GE               PG  G  GP                  +G+RG  GPKGE G +GP GPVG
Sbjct:  404 GLPGADGRAGVMGPAGSRGATGPAGVRGPNGDSGRPGEPGLMGPRGFPGSPGNIGPAGKEGPVGL------------PGIDGRPGPIGPAGARGEPGNIGFPGPKGPS---GDPGKAGEKGHAGLAGARGAPGPD------------------------GNNGAQGPPGLQGVQGGKGEQGPAGPPGFQGLPGPAGTAGEAGKPGER------------------GIPGEFGLPGPAGARGERGPPGESGAAGPTGPIGSRGPSGPPGPDGNKGEPGVVGAPGTAGPSGPSGL---------PGERGAAGIPGGKGEKGETGLRGDIGSPGRDGARGAPGAIGAPGPAGANGDRGEAGPAGPAGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGERGTKGPKGENGPVGPTGPVG 758          

HSP 4 Score: 72.7886 bits (177), Expect = 3.296e-11
Identity = 217/593 (36.59%), Postives = 262/593 (44.18%), Query Frame = 2
Query: 2489 GPPGTRGP---SGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAE---------------GQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQG---------------VEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPG---------------------KAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGL---DGKVGEPGQKGERYLK------------GIKGSPGTIGAVGSKGEPGPPGLTGPQGKRG------NPGPKGETGLMGPIGPVGEDGE 4042
            GP G RGP   SG PG+PG+ G  G  G  G++GP G+ G  G  GI G  G  GP G  G  G+ G  GP+GP+GD G+                   G  G  G  G +G  G  G  G  G  G++G+ G KG  GP GP G +G  GP G  G  G  GE GI G  G  G  G+RG +G  GE G+ G  G                       G  G  GP G  G KG+ G++G  G  G  G +G PG+ GA+G                 G PG  G +G  G +G  G  G +G  G  G  G  G  G  G  G  G  GP G                +GE G +GP   KGE GP+G  GPVG +GPSG +G PGP G  G  G  G  G PG  G                     K GL+GP G  G  G +GE G  GPPG    +G++GP+G  G  GP G  G  G +G  G +G PG +G RGL    G VGEPG  G                 G+ G+PG  G  G+ G  GPPG  G  G +G      N GP G  G  GP GPVG  G+
Sbjct:  425 GPAGVRGPNGDSGRPGEPGLMGPRGFPGSPGNIGPAGKEGPVGLPGIDGRPGPIGPAGARGEPGNIGFPGPKGPSGDPGK------------------AGEKGHAGLAGARGAPGPDGNNGAQGPPGLQGVQGGKGEQGPAGPPGFQGLPGPAGTAGEAGKPGERGIPGEFGLPGPAGARGERGPPGESGAAGPTG---------------------PIGSRGPSGPPGPDGNKGEPGVVGAPGTAGPSGPSGLPGERGAAGIPGGKGEKGETGLRGDIGSPGRDGARGAPGAIGAPGPAGANGDRGEAGPAGPAGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGERGTKGP---KGENGPVGPTGPVGAAGPSGPNGPPGPAGSRGDGGPPGATGFPGAAGRTGPPGPSGISGPPGPPGPAGKEGLRGPRGDQGPVGRSGETGASGPPGF---VGEKGPSGEPGTAGPPGTPGPQGLLGAPGFLGLPGSRGERGLPGVAGSVGEPGPLGIAGPPGARGPPGNVGNPGVNGAPGEAGRDGNPGNDGPPGRDGQPGHKGERGYPGNAGPVGAAGAPGPQGPVGPVGK 972          

HSP 5 Score: 61.6178 bits (148), Expect = 7.823e-8
Identity = 50/105 (47.62%), Postives = 58/105 (55.24%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVG---TRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            Y G  G  G++GA G  GP G VG    RGE G  G  G  G  GPRGP G  G++GD G  G+ GPRG  G KG  G  G PG AG+ G +G+ G VG  G  G
Sbjct:  948 YPGNAGPVGAAGAPGPQGPVGPVGKHGNRGEPGPAGAVGPAGAVGPRGPSGPQGIRGDKGEPGDKGPRGLPGLKGHNGLQGLPGLAGHHGDQGAPGAVGPAGPRG 1052          

HSP 6 Score: 59.3066 bits (142), Expect = 3.663e-7
Identity = 45/101 (44.55%), Postives = 58/101 (57.43%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G RG  G  G  GP+G VG RG  G +G++G+ G  G +GP G+ G+KG  G+QG  G  G  G++G  G+VGP   AG  G  G  GP G  GR G+
Sbjct:  971 GKHGNRGEPGPAGAVGPAGAVGPRGPSGPQGIRGDKGEPGDKGPRGLPGLKGHNGLQGLPGLAGHHGDQGAPGAVGP---AGPRGPAGPSGPAGKDGRIGQ 1068          

HSP 7 Score: 54.6842 bits (130), Expect = 9.584e-6
Identity = 193/549 (35.15%), Postives = 229/549 (41.71%), Query Frame = 2
Query: 2516 GLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPG------------RIGEAGPPGKTGASGAEGQPGV------------KGTKGDTGILGP---------------RGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGD------RGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPV 4027
            G PGKPG  G+ G +GP+G        G PG  G+ G +G  G  G  G KG  G  G +G  G  GE                                        G  G+ G RGL G +G VG  GP G +G  G +G  G  G  G  G  G  G  G  G  GP    G PG  G AG  GE G  G  G  GP G  G  G+PG  G  G  GV G  GL GP G PG             +GE GP G  G SG +G+PG             +G +G TG +GP               RG  G DG  G +G  G +G  G  G +G  G+SG  G  G +G +G PG  G +GP G  GPVG  G  G+ G  GP G              G PG  G  GP G SG  G+AGE+G       RG PG  GN G +GP G  G+                        +G +G  G  G PG +G   L G  G+ G  G  G +G PG  GL GP G RG  GP GE+G  GP GP+
Sbjct:  143 GHPGKPGRPGERGVVGPQG------ARGFPGTPGLPGFKGIRGHNGLDGLKGQPGAPGVKGEPGAPGEN---------------------------------------GTPGQTGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGP---VGNPGPAGPAGPRGEVGLPGLSGPVGPPGNPGANGLPGAKGAAGLPGVAGAPGLPGPRGIPGPVGAAGATGARGLVGEPGPAGSKGESGNKGEPGAVGQPGPPGPSGEEGKRGSTGEIGPAGPPGPPGLRGNPGSRGLPGADGRAGVMGPAGSRGATGPAGVRGPNGDSGRPGEPGLMGPRGFPGSPGNIGPAGKEGPVGLPGIDGRPGPIGPAGAR------------GEPGNIGFPGPKGPSGDPGKAGEKGHAGLAGARGAPGPDGNNGAQGPPGLQGV------------------------QGGKGEQGPAGPPGFQG---LPGPAGTAGEAGKPGERGIPGEFGLPGPAGARGERGPPGESGAAGPTGPI 604          
BLAST of Collagen type IV alpha 2 chain vs. UniProt/SwissProt
Match: sp|Q80ZF0|CORA1_RAT (Collagen alpha-1(XXVII) chain OS=Rattus norvegicus OX=10116 GN=Col27a1 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.364e-30
Identity = 313/890 (35.17%), Postives = 404/890 (45.39%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDG---KEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGV---RGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTG----------------------------ASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPG------RKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGP---NGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKR--STRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIY--GDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGC-MYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            RG PG RG  G  G  G DG   ++GS G KG +GPRG+ G PG QG  G RG SG  G LG  G  G  G +G  G+ G +G  G  GPPG  GP GP G  G PG  G  G+ G+ GP GP GE+G+   RG  G +G +GP+GPDG KGD G  G  G  G +G+ G+KG EG  G  G  G +G  G+PGS G+ G  G KG +G QG  G  G  G  G PG PGP+G +G       +GP GAPGR+G  G PG  G                              GA G PG +G  GD G  G  G+ G  G  G  G +G  G +G+PG  G  G  GP+G +GE G +G PG      R+G  G  GLPGP G  G  G +G  G  G            +PG  G+ G QG  G  G  G  G  G RG PG+AG  G +GP    G  G+ G  GP G+ G  G  G  GN G  GS+G  G  GE G  G   L G KG PG IG  G +G  GPPGL G +G  G PG  G +GL    GP G+ G  G  G  GP GPPGP G  G PG         +       +      G+  R ++  S   Q  +D    +F  +  ++N I+ I+ P G  K++ AR C D+    ++  DG Y++DPN G   D+IEV C F+   +TC+  +                     + + E A+SR            QMNFL   +S+  Q I   C    ++  G +  S     ++                           + +V+ +  G+  F   +  DGC ++      + F   T D   LPI+        + G     L V   CF
Sbjct: 1047 RGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGITGVRGREGKPGSQGEKGQRGAKGAKGYQGQLGEMGIPGDPGPPGTPGPKGSRGT------LGPMGAPGRMGAQGEPGLAGYNGHKGITGPLGPPGPKGEKGEQGEDGKTEGAPGPPGERGPVGDRGDRGEPGDPGYPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGASGRRGTQGLQGLPGPRGVVGRQGPEGMAGQDG------------NPGRDGRPGYQGEQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGESGLPGQLGPPGKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPGDIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGL---PGPKGDRGSRGDWGLQGPRGPPGPRGRPGPPGPPWHPVQFQQDDLEAAFQTWMDAHGAV-RLEQGYSYPDQLMLDQGGEIFKTLHYLSNLIQSIKTPLG-TKENPARVCRDLMDCEQKMADGIYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPI--------------------TASKAEFAVSR-----------VQMNFLHLLSSEGTQHITIHCLNMTVWQEGPARPSARQAVRFRA------------------------WNGQVF-EAGGQ--FRPEVSMDGCKVHDGRWHQTLFTFRTQDPQQLPIVSVDNLPPVSSG-KQYRLEVGPACF 1854          

HSP 2 Score: 83.5741 bits (205), Expect = 1.500e-14
Identity = 225/610 (36.89%), Postives = 274/610 (44.92%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPG---IDGKEGSIGPKGDL------------GPRGQSGNPGPQGIRGARGQSG------PTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGE---------KGDVGPLGKTGLKGTRGEVGIKGSEGKTGL---------------------------------MGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---------AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAG---------------------LQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GPPG  GP G+ GKPG   + G  G +GP+GD             G  G+ G PG  G+ G++G+ G      P GE G  G  G  G  G  G+KG+RG  G  G PGP G  G  G  G  GD G  G  G PG       +G+ G+ G KG  GP GPDG          KG VGP G+ G  G +G  G +G  G  G                                  MG +GP G  GEPG PGD+G+ G+ G  G  G QG  G  G  G+ G  GP G  G+KGD G  GP G  G  G+          GPPG TG  G EG+PG +G KG  G  G +G QG    LG +G  G  G  G PG +GS G  GP G  G +G QG PG  G  G  G+                       Q  D    G+PG PG+ G                     +QG  G  G QG+ G  G RG PG  G+ G+ GP G  G  G  G RG  G  G  GP G  G +G  G+ G+ G PG+ G    +G +G+ G  G VG  G  G PG+ G  G +G PG KGE+GL G +GP G+ G
Sbjct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

HSP 3 Score: 75.0998 bits (183), Expect = 6.633e-12
Identity = 194/545 (35.60%), Postives = 237/545 (43.49%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG---PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKV---GEPGQKGERYLKGIKGSPGTIGAV---------GSKGEPGPPGLT---------------GPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G  GP G PG  G  G++G  GP GD GP+G  G  G  G+ G  G  G  G  G  G RG+ G  G  GD GERG PG  G PG  G  G                             GV GLLG  G++GP+G   P G KG +G +G+ GLKG +GE G+ G  G  G  G +G  G+ G PG+ G  G                              P G+ G KG +GL GP G  G  G+ GPPG  G  G +G+PG +G  G  G LGP G++         G  G  G  G  G+ G  G  G  G++G  G  G PGR G +G  GL G VG  G  G  G  G  G  G  G  GP GS G PG  G  G  G  G  G  G RG  G  G  G+ G RGP+GP G                         +GS+GL G+V   G PGQ G++   G +G  G  G +         G+KG PG PG                 GP G  G PG  G++G+ G +GP+G  GE
Sbjct:  718 GQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGIPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPG---------------------------PPGVLGLLGDMGALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGFPGARGKPG------------------------------PMGKAGDKGSLGLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDE---------GPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAG------------------------EQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGE 1172          

HSP 4 Score: 72.0182 bits (175), Expect = 5.878e-11
Identity = 196/551 (35.57%), Postives = 251/551 (45.55%), Query Frame = 2
Query: 2498 GTRGPSGL---PGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGAS---GAEGQPGVKGTKGDTGI------------LGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER------YLKGIKGSPGTIGAVGSKGE---------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G RGP GL   PG+ G+ G  G +G  GD+G  G  G PGP+G++G  G     GE G KGD+G+ G  G +GD G +                                       G  G  G+ G  G +G  GP+G  G+KG +G  G  G +G  G++G  G  G  G+ G  G +G+ G PG  G  G                 GP+G  G+PG  G  G KG  G  GL G  G PG  G  GP G+ G     G  G+PG+ G KGD G+            +G  G  G  G  G  G  G  G RG PG +G+ G+ GP+G +G  G QG  G KG +GP G PG  G  G +G+ G  G  G  G  G  GP G+ GLPG+ G QGP G  G  GE G +G  G    PG+ G+ G +G  GP+G                        P G +GL G+ GEPG +G+        LKG +G PG  G  G KG+         PGPPG+TG +G+ G PG +GE G  G  G  G  G+
Sbjct:  790 GERGPPGLDGNPGEIGLPGPPGVLGLLGDMGALGPVGYPGPKGMKGLMGG---VGEPGLKGDKGEQGVPGVSGDPGFQ---------------------------------------GDKGSHGLPGFPGARGKPGPMGKAGDKGSLGLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEG---------------DEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLG------------------------PPGEQGLIGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGITGVRGREGKPGSQGEKGQRGAKGAKGYQGQ 1259          

HSP 5 Score: 67.781 bits (164), Expect = 1.149e-9
Identity = 49/109 (44.95%), Postives = 61/109 (55.96%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG------PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + G RG  G  G+KG+ GP G+ G  G  G +G +G  G+ GPRG      PEG+AG  G+ G  G  G +G+ GN GD G VGP G  GNPG+ G  G  G  G  GE
Sbjct: 1380 HPGPRGRPGPKGSKGEEGPKGKPGKAGASGRRGTQGLQGLPGPRGVVGRQGPEGMAGQDGNPGRDGRPGYQGEQGNDGDPGPVGPAGRRGNPGVAGLPGAQGPPGFKGE 1488          

HSP 6 Score: 65.855 bits (159), Expect = 3.706e-9
Identity = 46/102 (45.10%), Postives = 62/102 (60.78%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G+ G SG KGD GP G  G +G +G +G  G  G  GP GP+G+ G +GD G  GE G +G++G KG+EG  GPPG     G++G  G  G+QG  G++
Sbjct: 1150 GEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGIT---GVRGREGKPGSQGEKGQR 1248          

HSP 7 Score: 65.4698 bits (158), Expect = 4.812e-9
Identity = 139/400 (34.75%), Postives = 163/400 (40.75%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP---GKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---PGPKGETGLMGPIGPVGEDG 4039
            G  G  G KG  G  G  GE+G  GP G  G KG  G  G  G  G  G  GSRG  G+ G  G PG  G  G                                G+ G  G +G  G PG  GE GPP   G  G  G  G PGV G  GD G LGP G                                GP+G++G +G  G PG KG+ G  G+PG  G  G  G  G+ G                PG PG  G  GP G +G +G  G  G  GP G  G++G  G NGP G+                      G  G+RGL G  G+ G +G+    G  G PG  G  G KG PG PGL G +G+ G+   PGP GE GLMG +G VGE G
Sbjct:  697 GPMGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGL------------------------------PGIPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLLGDMGALGPVG------------------------------YPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGL---------------PGFPGARGKPGPMGKAGDKGSLGLPGPPGPEGFPGDIGPPGDNGPEGM---------------------KGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPG 1000          

HSP 8 Score: 62.3882 bits (150), Expect = 5.305e-8
Identity = 181/526 (34.41%), Postives = 212/526 (40.30%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQ------SGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXX------------------XXXXSRGLDGKVGEPGQKGER---YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG 3994
            G  G  GL G PG  G++G  G  G  G  G+ G PGP+G  GA+G        GP G+ G KG RG  G  G  G  G                                               G RGL G  G  G +G  G  GD G  G  GL G  GE+G+ G  G  GL+G  G  G  G PG  G  G+ G  GE G                         KG KG+ G+ G +G PG  G+ G  G  G  GA G+PG  G  GD G L                     G  G PG +G  G+ GP G  G  G++G PG +G  GP G  GP G  GP G  G PG                 G PG+ G  G  G  G++GE G+ G  GP G  G MG  G  G  GIVG  G RG  GP G  GP G+                   GP GSRGL G  G  G +G R      G +GS G  G VG +G PG PG  G  G+RG+ G KG
Sbjct:  685 GNMGLPGLAGNPGPMGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPG---------------------------------------------SDGERGLPGIPGKRGEMGRPGFPGDFGERGPPGLDGNPGEIGLPGPPGVLGLLGDMGALGPVGYPGPKGMKGLMGGVGEPGL------------------------KGDKGEQGVPGVSGDPGFQGDKGSHGLPGFPGARGKPGPMGKAGDKGSL---------------------GLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLE---------------GQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKG 1105          

HSP 9 Score: 58.9214 bits (141), Expect = 4.737e-7
Identity = 48/111 (43.24%), Postives = 55/111 (49.55%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP---------PGYAGNPGIKGSRGPVGNQGRDG 2131
            Y G  G++G  G  G  G  G  G RG  G KG KGE G +G  G  G +G +G  G+QG  GPRG  G +G EG  G          PGY G  G  G  GPVG  GR G
Sbjct: 1359 YPGQEGVQGLRGEPGQQGQPGHPGPRGRPGPKGSKGEEGPKGKPGKAGASGRRGTQGLQGLPGPRGVVGRQGPEGMAGQDGNPGRDGRPGYQGEQGNDGDPGPVGPAGRRG 1469          

HSP 10 Score: 57.7658 bits (138), Expect = 1.128e-6
Identity = 194/550 (35.27%), Postives = 232/550 (42.18%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGP------------DGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEP---GSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXE---------KGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRG------EQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV---------------GSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            G  GP G PG  G+ G  G +G  G  G +G+ G PG  G  G +G  G  G  G  G RGK GP G  GDKG                 G  G  G  G  G  G  G+ GP+G  G+ G RGL G  G +GP G             +G+ G  G  G+ GL G++GE G  G  G  G  G  G  G+ GEP   G  GD GV G  G  G +GS G  G  G  G PG+PGP G          +G KG  G  GP G  G  G  G  G+ G  G  GQPG +G  G+ G  G +G        G  G  G  G  G +G +G  G  G  G  GP G  GE G+ G  G KG+LGP+G PG  G                       G  G PGL G  G  GP G  G +G+ G  G+ G  G  G  G+ G  GP GI G                         RG +GK G  G+KG+R  KG KG  G +G +               GS+G  GP G  G  G +G PG  G  G  G
Sbjct:  820 GALGPVGYPGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGFPGARGKPGPMGKAGDKGS---------------LGLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDEGPMGPPGVPGLEGQPGRKGFPGRPGLDGSKGEPGDPGRPGPVGEQGLMGFVGLVGEPGIVGEKGDRGVMGPPGAPGPKGSMGHPGTPGGVGDPGEPGPWGPPGSRGLPGMRGAKGHRGPRGPDGPAGEQGSKGLKGRVGPRGRPGQPGQQGAAGERGHSGAKGFLGIPGPSGPPGAKGLPGEPGSQGPQGPVGPPGEMGPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGL---------------------IGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGITGV------------------------RGREGKPGSQGEKGQRGAKGAKGYQGQLGEMGIPGDPGPPGTPGPKGSRGTLGPMGAPGRMGAQGEPGLAGYNGHKG 1309          

HSP 11 Score: 56.225 bits (134), Expect = 3.397e-6
Identity = 47/103 (45.63%), Postives = 54/103 (52.43%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + GA+G  G  G  G+ GP G+ G +G  G  G  GE   QG  GPEG  G KG  G QG  GP G  G KG  G +G PG  G PG  G RG  G  G+ GE
Sbjct:  680 HDGAKGNMGLPGLAGNPGPMGRKGHKGHPGAAGHPGE---QGQPGPEGSPGAKGYPGRQGFPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGIPGKRGE 779          

HSP 12 Score: 55.8398 bits (133), Expect = 4.639e-6
Identity = 105/333 (31.53%), Postives = 134/333 (40.24%), Query Frame = 2
Query: 3173 GVKGDIGLIGPTGAPG---RIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGP---PGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGI------------KGSPGTIGAVGSKGEP---------------------------GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGED 4036
            G KG++GL G  G PG   R G  G PG  G  G +GQPG +G+ G  G  G +G  G                          G+ GP+G  G +G+ G    PG  GE G  G+PG  G  G                         PG PG  G +GP    G  G  GE G  GPPG+ G +G  G  GP+G                      PGPKG +GL G VGEPG KG++  +G+            KGS G  G  G++G+P                           GPPG  GP+G +G PG +G  G  G +GP G++
Sbjct:  682 GAKGNMGLPGLAGNPGPMGRKGHKGHPGAAGHPGEQGQPGPEGSPGAKGYPGRQGFPG------------------------PVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGIPGKRGEMG------------------------RPGFPGDFGERGP---PGLDGNPGEIGLPGPPGVLGLLGDMGALGPVGY---------------------PGPKGMKGLMGGVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSHGLPGFPGARGKPGPMGKAGDKGSLGLPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPPGQLGPEGDE 942          

HSP 13 Score: 55.0694 bits (131), Expect = 7.371e-6
Identity = 44/100 (44.00%), Postives = 53/100 (53.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY---AGNPGIKGSRGPVGNQG 2122
            G RG  G +G  G+ G  G  G  G+ G  G  G  G+ GP+GP G      DIG +G  GPRG  G  G EG +GPPG    +G PG KG RG  G+ G
Sbjct: 1499 GKRGTEGGTGLPGNQGEPGSKGQPGDSGEMGFPGVAGLFGPKGPPG------DIGFKGIQGPRGPPGLMGKEGIIGPPGMLGPSGLPGPKGDRGSRGDWG 1592          

HSP 14 Score: 54.6842 bits (130), Expect = 9.981e-6
Identity = 44/105 (41.90%), Postives = 53/105 (50.48%), Query Frame = 2
Query: 1868 GDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP---------------PGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  GP G VG  G  G  G+KG+ G  GP G +G+ G +G+ G++G+ GP G DG KGD G  GP                G  G PGI G RG  G  G  GEK
Sbjct: 1141 GPKGPPGAVGEPGLPGDSGMKGDLGPLGPPGEQGLIGQRGEPGLEGDLGPVGPDGLKGDRGDPGPDGEHGEKGQEGLKGEEGLPGPPGITGVRGREGKPGSQGEK 1245          
BLAST of Collagen type IV alpha 2 chain vs. TrEMBL
Match: A0A5J4NUW8 (Collagen, type V/XI/XXIV/XXVII, alpha OS=Paragonimus westermani OX=34504 GN=DEA37_0001678 PE=4 SV=1)

HSP 1 Score: 399.823 bits (1026), Expect = 1.064e-110
Identity = 399/905 (44.09%), Postives = 517/905 (57.13%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---------------------AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQ---------------------GPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAG---------------GSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRA---QSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE--VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYG-DSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            RG PG RG  G PG+PG++G EG  G KGD GP G +G  GP G+RG+ G +GP GELG KGD+G+ G  G  GDKG+RG PG+QG PG  GP GP G+ GSPG+ G KG  G PGP G  GEKGVRGL+GR+G+ GPIG DGE GD G  G  G KG RG+ G +G +G  G +G+ GPQGV+GEPG  G  GV                      G  G  G  GE G  G +G+ GP GA G  GE                      G PGK G +G  G PG+KG+KG+ GILGPRG QG  G  GP G  G KG RGKPGRQG  G +GP G+ G+ G Q                     GPPG+ G  GPIGL GP G  GP G  G  G  G  G  G  G  G+PG PG  G  GP G                SG  G  GE+G +GPPGIAG  G  G  G +G                                G  GEPG      +KG+ G+PGT+G  G KGE G  G  GP G++G  GP+GE G  GP GP G+DGE G  G  GP GPPGP G RG PG  GD G  GRPGP GQVK+ N PE    R +R+  A   Q+  D+N  +F ++D I  +++  R P G + +  ARTC DI+L  K + +  Y+IDPNEGS +D+++V+C F ++  V+TC+ S   QG L  Y KP+ G++ WQ+ LR+  + +++PDLH+ Y DH+Q+N LR Q+ ++ QE+ FLCD + IYG   +   E++F YAT L+ H+ + +DL  GERLG    +MDKR+Y++   R   ++ +EYDGC Y + G+S+K ++ET+DL++LPIIDF++  Y+ +G+S+L LNV ++C+
Sbjct:  782 RGMPGPRGAPGNPGRPGVEGVEGPQGDKGDAGPLGPTGPSGPVGLRGSPGPTGPPGELGPKGDQGRPGMPGSIGDKGDRGPPGAQGSPGVLGPAGPQGMPGSPGEPGSKGARGQPGPIGARGEKGVRGLIGRQGAHGPIGSDGETGDQGSPGLQGAKGDRGDTGEEGPQGPPGDVGAAGPQGVAGEPGEAGPPGVT---------------------GPKGIEGKVGEMGPIGPMGVQGPPGANGPRGEPGTKGVPGPDGPPGDVGPPGLTGAPGKVGNAGPPGLPGIKGSKGEPGILGPRGLQGPAGKAGPEGPIGPKGNRGKPGRQGQPGPAGPPGILGDPGFQGVPGIQGPPGPSGKPGPQGSQGPPGKDGVRGPIGLDGPFGEDGPPGPPGPAGAAGPQGPVGSAGETGAPGDPGSRGPPGPVGTDGEPGLPGPPGPEGPSGPTGPQGEQGFQGPPGIAGPKGNVGKQGAVGPT------------------------------GASGEPG------MKGVTGNPGTVGPTGPKGEQGSIGDPGPAGQQGERGPEGEIGPFGPRGPAGDDGEPGPQGRQGPPGPPGPMGPRGKPGQPGDPGPMGRPGPQGQVKVTNAPEPGLRRRRRAVSAQVEQTLEDLNLKIFQRIDAIHKRVRLARQPAGSSPNYPARTCKDIRLANKLARNDTYWIDPNEGSSRDTVKVKCNFYDDGRVETCVESTMDQGILATYMKPLPGDSEWQSQLRVINS-TKSPDLHT-YGDHSQVNMLRIQHRRASQELLFLCDGTAIYGLWDDRRHESEFDYATALLAHNGRMLDLRAGERLGFGLDHMDKRIYRNALQRADTSIIVEYDGCQYMSTGASTKIRVETSDLNVLPIIDFKVRSYDRQGLSSLSLNVGAVCY 1627          

HSP 2 Score: 219.935 bits (559), Expect = 9.676e-54
Identity = 152/407 (37.35%), Postives = 212/407 (52.09%), Query Frame = 2
Query:  254 DGVVDLLTAVQISDKNIGTTAEEP-FCGNS----RRKAFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRT--SDPLDI-SGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNN--DFNDVMMNQGPVKXXXXXXXXXXXXXXXXXXXXXXXXXNGRDGLPGTPGVPGAPGHIFVLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGL------------------------TGPKGRSGDIGMQGLPGNK 1372
            +G +DLL AV I          +P FC  S    +  A+ Y  G+SL   +  +F  K P +FSIL T + ++  +Q +F + +     +LAV+ G  ITL Y   S      + F + L   +WHRI+ SV   SV+LL+DC+ + S  L R     PL I  G  L+VLQ  +G  SF+GI+D LLL+     A+  C +Y+P CN    FN+ M ++                          EG++GR G+PG+PG+PG   H+ V+PIP   +N   R+A FR+ ++++  +LKG RG QGM GLPG QGP+GY G KG+ G  GD GF+GP GLRG  G  GP GR G DGE G++GL                         GP+G  GDIG +G PG +
Sbjct:   56 EGSIDLLKAVSIDLAAPTDVIRQPGFCPPSDEQLQHTAYSYHRGSSLRVFATKIFADKLPTDFSILATVRLKSTGAQKLFDMVDGKQQALLAVSVGPIITLHYY--SQGKLHLVEFSYPLEVNRWHRISLSVKGDSVTLLVDCRVVQSKSLQRPIPVPPLAIPDGSTLHVLQTADGKNSFSGIVDRLLLIPNPEGAYDHCTVYSP-CNRVAYFNERMQSE-------------------------DEGRDGRSGIPGSPGIPGVASHVLVIPIPSFGKNEMSRMASFRSVVQQSVMMLKGIRGQQGMVGLPGIQGPVGYVGEKGEQGVVGDPGFMGPTGLRGPPGPDGPRGRPGVDGEVGTSGLPGAKGQPGLPGLPGLPGPKGHKGDIGPQGNKGDIGKRGAPGPR 434          

HSP 3 Score: 112.464 bits (280), Expect = 8.794e-21
Identity = 209/563 (37.12%), Postives = 254/563 (45.12%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRI---------------------------------GEAGPPGK------TGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGPPG RG  G PG PG  G +G+ G KG+ G  G  G+ GP G  G +G  GP G  GSKGD+   GP G TG +GER                                          G+KG +G  G  G  G  GP G KG+ G +G+ G +G  G+ G +G+ G  G  G +GP+G  G PG  G  G  G+ G  G  G +GP GP G  G  G PG  G  G+KG+ G +GPTG  G +                                 GEAG PG+       G  GA GQ G +G+ G+ G+ GPRG  G                           N G  GVEG  G QG  G  G LGP G  GPVG        G+PGP G  G+ G  G  G PG+PG  G +G  G  G QG  G  G  GP      PG  G+ G RG  GPIG  G  G RG  G  G  GPIG+ G  G +G  G  G  G +G+   +G +G PG +GA G +G  G PG  GP G  G  G +G+ G MGPIGP+G  G
Sbjct:  515 RGPPGARGLVGSPGLPGNPGPQGNAGAKGEPGATGPIGDTGPMGPPGYKGDRGPRGPAGSKGDQ---GPPGKTGVRGER------------------------------------------GDKGTKGERGNPGPRGSDGPVGIKGETGMVGEPGPRGLEGQKGAQGARGPAGYGGIQGPKGALGLPGPIGRTGEKGDPGPVGPLGPRGPPGPRGAPGPRGSPGNSGPVGMKGEDGPVGPTGVLGPVGPQGPRGVPGFAGGPGPVGLPGRPGPPGEIGERGEAGEPGQPGEVGPIGMVGAPGQTGEQGSPGERGMPGPRGAPG---------------------------NPGRPGVEGVEGPQGDKGDAGPLGPTGPSGPVGL------RGSPGPTGPPGELGPKGDQGRPGMPGSIGDKGDRGPPGAQGSPGVLGPAGPQGMPGSPGEPGSKGARGQPGPIGARGEKGVRGLIGRQGAHGPIGSDGETGDQGSPGLQGAKGDRGDTGEEGPQGPPGDVGAAGPQGVAGEPGEAGPPGVTGPKGIEGKVGEMGPIGPMGVQG 999          

HSP 4 Score: 97.4413 bits (241), Expect = 2.987e-16
Identity = 235/570 (41.23%), Postives = 286/570 (50.18%), Query Frame = 2
Query: 2495 PGTRGP---SGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKG------VRGLLGRKGSVGPIGPDGEK------------GDVGPLGKTGLKGTRGEVGIKGSE------GKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGP---RGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKA---GLQGPAGGSGTQGEAGERGDRGPPGIAGN------------MGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGP---KGETGLMGPI------GPVGEDGE 4042
            PG +G     GLPG PG  G +G IGP+G+ G  G+ G PGP   RG  G+ GPTGE G  G +G FGP GP G +G  G  G  G  G  G  G +G  G  G  G  G+ G  GP+G  G+ G       RGL+G  G  G  GP G              GD GP+G  G KG RG  G  GS+      GKTG+ G RG +G  GE G+PG  G +G                                G+KG+ G++   G PG  G  G  G  GA G  G  G++G KG  G+ GP    GE+G  GP+GP+G +G  G RG PG +GS GNSGP G++GE G  GP G  G +GP G  G  G +G  G  G PG PG  G+ G+ G  G PG PG+    G+ G  G +G QG  GERG  GP G  GN             G +G  GP+G  GP GP G  G  GP GP G  GPKG +G  G  G  G KG+R   G +GSPG +G  G +G PG PG  G +G RG PGP   +GE G+ G I      GP+G DGE
Sbjct:  392 PGAKGQPGLPGLPGLPGPKGHKGDIGPQGNKGDIGKRGAPGP---RGTEGEVGPTGEPGPAGTQGSFGPVGPPGVRGSPGRVGPSGMKGEMGDAGAVGPPGPRGPPGPPGMDGPQGPRGLSGQPGSRGPPGARGLVGSPGLPGNPGPQGNAGAKGEPGATGPIGDTGPMGPPGYKGDRGPRGPAGSKGDQGPPGKTGVRGERGDKGTKGERGNPGPRGSDGPV------------------------------GIKGETGMV---GEPGPRGLEGQKGAQGARGPAGYGGIQGPKGALGLPGPIGRTGEKGDPGPVGPLGPRGPPGPRGAPGPRGSPGNSGPVGMKGEDGPVGPTGVLGPVGPQGPRGVPGFAGGPGPVGLPGRPGPPGEIGERGEAGEPGQPGEVGPIGMVGAPGQTGEQGSPGERGMPGPRGAPGNPGRPGVEGVEGPQGDKGDAGPLGPTGPSGPVGLRGSPGPTGPPGELGPKGDQGRPGMPGSIGDKGDRGPPGAQGSPGVLGPAGPQGMPGSPGEPGSKGARGQPGPIGARGEKGVRGLIGRQGAHGPIGSDGE 925          

HSP 5 Score: 95.1301 bits (235), Expect = 1.376e-15
Identity = 82/141 (58.16%), Postives = 97/141 (68.79%), Query Frame = 2
Query: 1715 GSAGLPGNPXXXXXXXXXXXXXXXXXXXXXXXXXXXXYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GS GLPGNPG QG+ G KG+ G +GP G+ G  GPPGYKG RG RG +G+KGD GP G+ G RGE G KG KGE G  GPRG +G  G+KG+ G+ GE GPRG +G KG +G+ GP GY G  G KG+ G  G  GR GEK
Sbjct:  525 GSPGLPGNPGPQGNAGAKGEPGATGPIGDTGPMGPPGYKGDRGPRGPAGSKGDQGPPGKTGVRGERGDKGTKGERGNPGPRGSDGPVGIKGETGMVGEPGPRGLEGQKGAQGARGPAGYGGIQGPKGALGLPGPIGRTGEK 665          

HSP 6 Score: 69.3218 bits (168), Expect = 1.074e-7
Identity = 149/414 (35.99%), Postives = 183/414 (44.20%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQ---------GLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTI---GAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGP------IGPVGEDGE 4042
            +G +G++G  G  GP+G  GEKG+ G +G  G  G  G  G  G +G  G  G  G  G SG PG+                         G  G+PG PG  G KG KGDIG  G  G  G+ G  GP G  G  G  G+PG  GT+G  G +GP G +         G+ G +G  G+ G  G RG PG  G  G  GP+G+ G+ G +GPPG +G +G                                    SPGLPG  G QG AG  G  G  G  GD GP G  G  G RGP GP G                    G+ GP G  G+ G+ G+ G KGER   G +GS G +   G  G  GEPGP GL G +G +G  GP G  G+ GP       GP+G  GE
Sbjct:  329 RGQQGMVGLPGIQGPVGYVGEKGEQGVVGDPGFMGPTGLRGPPGPDGPRGRPGVDGEVGTSGLPGA------------------------KGQPGLPGLPGLPGPKGHKGDIGPQGNKGDIGKRGAPGPRGTEGEVGPTGEPGPAGTQGSFGPVGPPGVRGSPGRVGPSGMKGEMGDAGAVGPPGPRGPPGPPGMDGPQGPRGLSGQPGSRGPPGARGLVG------------------------------------SPGLPGNPGPQGNAGAKGEPGATGPIGDTGPMGPPGYKGDRGPRGPAG------------------SKGDQGPPGKTGVRGERGDKGTKGERGNPGPRGSDGPVGIKGETGMVGEPGPRGLEGQKGAQGARGPAGYGGIQGPKGALGLPGPIGRTGE 664          

HSP 7 Score: 65.0846 bits (157), Expect = 1.886e-6
Identity = 47/100 (47.00%), Postives = 59/100 (59.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            GARG+ GS G  G+ GP G  G +GE G  G  G+ G  GP G +G  G +G  G +G+ GP GK G +G+ G  G  G  GNPG +GS GPVG +G  G
Sbjct:  519 GARGLVGSPGLPGNPGPQGNAGAKGEPGATGPIGDTGPMGPPGYKGDRGPRGPAGSKGDQGPPGKTGVRGERGDKGTKGERGNPGPRGSDGPVGIKGETG 618          
BLAST of Collagen type IV alpha 2 chain vs. TrEMBL
Match: A0A4Z2DJT0 (Collagen alpha-1(V) chain isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_000274 PE=4 SV=1)

HSP 1 Score: 379.022 bits (972), Expect = 4.263e-105
Identity = 405/849 (47.70%), Postives = 517/849 (60.90%), Query Frame = 2
Query: 2528 KPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQ---------GPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ---GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEP---GQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQ---SFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE--VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGD-SELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            KPG++G EG  G KG  G  G SG  GP G+RG RG  GP+G+ G+KGD G+ GP G  GDKG+RG PG  GPPGP GP GP G+ GS G+ G KG  G PGP G  GEKGV+GL+G+ G  GP G DGE+GD G  G  G KG RGE G +GS G+ G  GS GPQG+ GEPG  G+AGV G KG +GK G  GP GP GVQG PG+ GPRGE G+KG IG  GP G PG +G AG PGK G  GA G PG+ G KG+ G  G RGEQG  G +GP G KG+KG  G+PGRQG  G++GP G+ GE G+Q         G PG  GE G  GLPG  G  G  G +G  G  G  G  G  G  G  G PG AG  GP G  G +   G  G  G+RGPPGI G  GQ GP GP G                 G  G PG  G +G  GK+G P   G KGE   KG+ G+PGT+G  G KGE G  G  GP GK+G  G  G+ G  GP GP G++GESG  GP+G  GPPG  G RG  G SGD G  G+ GP GQVKI N  E    RYKRS   Q   +   IN  +F +VD +  K++  R+ TG +  + ARTC D++L  K + +  Y+IDPNEGS +D+I  +C F ++  V+TC+ S   QG L  Y KP+  ++ WQ+ LR+  + +   DLH+ Y  H+Q+N LR Q+  + QE+EFLCD + IYG  +  + ++ F  AT L+ H+ + +DL+ GERLGP + +MDKR Y++   R   ++T+EYDGC Y T G+++K  +ET DL +LPIIDF++ +++ +   +L +NV ++C+
Sbjct:  500 KPGVEGIEGVQGEKGTKGAPGPSGPSGPVGLRGTRGPPGPSGDPGAKGDEGRRGPHGSLGDKGDRGPPGPHGPPGPIGPVGPQGLPGSQGEPGDKGARGPPGPVGETGEKGVQGLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGEIGNAGVPGPKGIEGKVGPPGPPGPAGVQGSPGRSGPRGEPGIKGVIGAEGPVGFPGPVGLAGAPGKMGVPGAPGSPGLPGLKGEQGKPGARGEQGAQGAIGPAGPKGMKGMLGRPGRQGQPGSAGPPGIVGEPGVQGAQGPMGPIGAPGLPGEPGSAGLPGKDGVRGDIGPEGIFGDDGPPGPPGPVGVPGVQGPPGTAGETGPIGDPGDRGAPGSIGSPGERGPPGIMGAEGQVGPQGPEG---------------EQGLQGPPGDSGPKGNIGKIGSPGPVGPKGESGPKGVGGNPGTVGPPGPKGEQGSIGDPGPSGKQGEMGGHGDQGPPGPRGPNGDEGESGPPGPIGQPGPPGLQGPRGEQGHSGDPGPMGKQGPQGQVKITNNGENLRMRYKRSAVVQTEETLEQINQNIFGRVDALNKKVRSRRYATGLSPQNPARTCKDVRLTNKFAQNDTYWIDPNEGSNRDAIRAKCTFYDDGTVETCVDSSMDQGILVTYLKPLPSDSEWQSQLRVMNSTTPL-DLHN-YGSHSQVNMLRIQHRYASQELEFLCDGTEIYGSWNPRTSQSDFSKATSLLAHNERMLDLTSGERLGPGKYHMDKRNYRNNLERVDTSITVEYDGCQYLTRGAATKLLIETRDLEVLPIIDFKVRNFDKQDTCSLSINVGAVCY 1331          

HSP 2 Score: 86.2705 bits (212), Expect = 6.391e-13
Identity = 166/389 (42.67%), Postives = 195/389 (50.13%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGV------------------SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG---------------------------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVG 3517
            RGPPG  G +G  G  G+ GK G  GP G  G +G  G+PGPQG +G RG+ G  G  G +G  G  GPQG  G+ GE       G  G  GP G  G  G PG  G  GV G+PG  GP GE G++G++G +G VG  GP G  G  G +G  G  G+ G  G+KG +GK G  G +G QG                    G+PGS G  G+ GE G QG QG  GP G  G+ G PG  G  G+ GV+GDIG                             G TG  G  G+ G PG  G+ G  G PG+ G +G  G  GP GEQGL GP G  G KG  G  G PG  G  G SGP+GV G  G  GPPG KGE G IG PGP G
Sbjct:  597 RGPPGPVGETGEKGVQGLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGE------IGNAGVPGPKGIEGKVGPPGPPGPAGVQGSPGRSGPRGEPGIKGVIGAEGPVGFPGPVGLAGAPGKMGVPGAPGSPGLPGLKGEQGKPGARGEQGAQGAIGPAGPKGMKGMLGRPGRQGQPGSAGPPGIVGEPGVQGAQGPMGPIGAPGLPGEPGSAGLPGKDGVRGDIGPEGIFGDDGPPGPPGPVGVPGVQGPPGTAGETGPIGDPGDRGAPGSIGSPGERGPPGIMGAEGQVGPQGPEGEQGLQGPPGDSGPKGNIGKIGSPGPVGPKGESGPKGVGGNPGTVGPPGPKGEQGSIGDPGPSG 979          

HSP 3 Score: 73.559 bits (179), Expect = 4.774e-9
Identity = 71/173 (41.04%), Postives = 88/173 (50.87%), Query Frame = 2
Query: 1031 VLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLT------------------------GPKGRSGDIGMQGLPGNKXXXXXXXXXXXXXXXXXXXXXXASGRPGDVGPPGE 1477
            ++P+P   +N   R+A FRT++++   +LKG+RG QGM GLPG QGP G RG KGD G  G+ GFIGP GLRG  G  GP GR G DGE G++GL                         GP G  GDIG++G PG                          GR G+VGPPGE
Sbjct:    1 MMPLPSFGKNELSRMASFRTTVQQAVMMLKGSRGQQGMIGLPGIQGPPGPRGSKGDQGLIGEPGFIGPTGLRGPPGPVGPRGRPGVDGEVGASGLAGPKGLPGLPGLPGLPGHRGHKGEIGPSGIKGDIGLRGPPG------------------------PYGRDGEVGPPGE 149          

HSP 4 Score: 71.633 bits (174), Expect = 2.074e-8
Identity = 224/641 (34.95%), Postives = 270/641 (42.12%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGP---------------------TGELGSKGDRGKFGP---QGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGS---------------------VGPIGPDGEKGD---VGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ------------GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---------------AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGEL------------------------GIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE------RGDRGPPGIAGNMGQRGPN---GPIGIVXXXXXXXXX---------XXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGPPG  G  G PG  G  G +G+ GPKG+ G  G+ G+ GP G  G +G  GP                      GE GSKG+RG  GP   +GP G KGE                                        G IGE G RGL G+KG+                      G IG  GEKGD   +GPLG +G  G RG  G +G+ G++G +G +G +            G  G  G+PG AGV          G  G  G  G  G+ G  G  GE G+ G+IG IG  G PG++GE                G PGK G  G EG  G KGTKG  G  GP G  GL       G++G  G  G PG +G  G  GP G  G+                         G QG PG KG  GP   PGPVG +G  G                       GL GK GL+GP G  G QG+ G       +GDRG PG  G+ GQ+G     GP G+VG  G  G           G +GP GP G  G +GS G  G  GEPG KG    +G  G PG +G  G+ G+      PG PGL G +G++G PG +GE G  G IGP G  G
Sbjct:  219 RGPPGVTGLPGSPGMTGNPGPQGNAGPKGEPGLTGEPGDTGPVGASGYKGDRGPRGLPGAKGAQGAAGLTGVRGDRGEKGSKGERGLPGPRGSEGPVGIKGE---------------------------------------PGIIGELGPRGLEGQKGNEGPRGSPGYPGGPGPKGTVGPAGGIGSPGEKGDPGHIGPLGLSGPVGPRGAPGPRGAPGQSGPVGFKGDEGPTGPTGPPGAAGPQGPRGTPGFAGVP---------GPPGLPGRPGPVGLLGDRGEPGEPGIPGEIGPIGMVGVPGQVGEQGAPGERGPPGPRGPQGIPGKPGVEGIEGVQGEKGTKGAPGPSGPSGPVGL------RGTRGPPGPSGDPGAKGDEGRRGPHGSLGDKGDRGPPGPHGPPGPIGPVGPQGLPGSQGEPGDKGARGP---PGPVGETGEKGVQ---------------------GLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGEIGNAGVPGPKGIEGKVGPPGPPGPAGVQGSPGRSGPRGEPGIKGVIGAEGPVGFPGPVGLAGAPGKMGVPGAPGSPGLPGLKGEQGKPGARGEQGAQGAIGPAGPKG 781          

HSP 5 Score: 70.8626 bits (172), Expect = 3.621e-8
Identity = 226/611 (36.99%), Postives = 263/611 (43.04%), Query Frame = 2
Query: 2486 RGPPGTR---GPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIR------------------------------------GARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLG------------KTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDA---GVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGIL---------GPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGL---PGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDR------GPPGIAGNMGQRGPNGPIG---------IVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYL---------------KGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVG 4030
            RGPPG     G  G PG+PG  G +G +GP G  G RG  G  GP G++                                    GARG  G TG  GS G  G  GPQG  G KGE                                                 GL G  G  GP+G  G KGD GP G             TG++G RGE G KG  G  G  GS GP G+ GEPG  G+    G+ G+KG +G +GS G  G  G +G  G  G  G  G KGD G IGP G  G +G  G PG  GA G  G  G KG +G TG           GPRG  G  G  GP G  G  G  G               + G+ G  G PG  GE+GPIG+   PG VG  G  G+ G PGP G  G  G  G  G  G+ G+ G +G  G SG  G  G RG R      G PG  G+ G+RGP+G +G           GP GP G  GP G  G  G PG KG+RG  G VGE G+KG + L               +G +GSPG  GA G +GEPG  G TG QG  G+PGP+G  G  G IG  G
Sbjct:  132 RGPPGPYGRDGEVGPPGEPGSAGLQGPMGPVGPAGLRGLPGRSGPNGLKGEFGDSGEIGPPGPQGPVGPPGVPGPQGIRGLTGKPGARGPPGVTGLPGSPGMTGNPGPQGNAGPKGE------------------------------------------------PGLTGEPGDTGPVGASGYKGDRGPRGLPGAKGAQGAAGLTGVRGDRGEKGSKGERGLPGPRGSEGPVGIKGEPGIIGELGPRGLEGQKGNEGPRGSPGYPGGPGPKGTVGPAGGIGSPGEKGDPGHIGPLGLSGPVGPRGAPGPRGAPGQSGPVGFKGDEGPTGPTGPPGAAGPQGPRGTPGFAGVPGPPGLPGRPGPVG---------------LLGDRGEPGEPGIPGEIGPIGMVGVPGQVGEQGAPGERGPPGPRGPQGIPGKPGVEGIEGVQGEKGTKGAPGPSGPSGPVGLRGTRGPPGPSGDPGAKGDEGRRGPHGSLGDKGDRGPPGPHGPPGPIGPVGPQGLPGSQGEPGDKGARGPPGPVGETGEKGVQGLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGEIGNAG 679          

HSP 6 Score: 67.781 bits (164), Expect = 2.769e-7
Identity = 138/294 (46.94%), Postives = 161/294 (54.76%), Query Frame = 2
Query: 2879 RKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGIL---------GPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRG 3724
             +G  G IGP G KGD+G  G  G  G  GEVG  G  G  GL G  GP G +G  G PG +G NG KGE G  G  GP GP G  G PG PGP+G +G+ G  G  GP   TG PG  G  G PG  G +G +G+PG+ G  GDTG +         GPRG  G  G  G  G  G++G RG+ G +G  G  GP+G EG +GI+G PG  GELGP GL G  G  GP G  G PG PG  G  G  G +GSPG  G  G  GP G SG  G  G  G RG PG +G +G +G
Sbjct:  113 HRGHKGEIGPSGIKGDIGLRGPPGPYGRDGEVGPPGEPGSAGLQGPMGPVGPAGLRGLPGRSGPNGLKGEFGDSGEIGPPGPQGPVGPPGVPGPQGIRGLTGKPGARGPPGVTGLPGSPGMTGNPGPQGNAGPKGEPGLTGEPGDTGPVGASGYKGDRGPRGLPGAKGAQGAAGLTGVRGDRGEKGSKGERGLPGPRGSEGPVGIKGEPGIIGELGPRGLEGQKGNEGPRGSPGYPGGPGPKGTVGPAGGIGSPGEKGDPGHIGPLGLSGPVGPRGAPGPRGAPGQSGPVGFKG 406          
BLAST of Collagen type IV alpha 2 chain vs. TrEMBL
Match: A0A4Z2DJH5 (Collagen alpha-1(V) chain isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_000274 PE=4 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 2.064e-104
Identity = 405/849 (47.70%), Postives = 517/849 (60.90%), Query Frame = 2
Query: 2528 KPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQ---------GPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ---GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEP---GQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQ---SFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE--VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGD-SELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            KPG++G EG  G KG  G  G SG  GP G+RG RG  GP+G+ G+KGD G+ GP G  GDKG+RG PG  GPPGP GP GP G+ GS G+ G KG  G PGP G  GEKGV+GL+G+ G  GP G DGE+GD G  G  G KG RGE G +GS G+ G  GS GPQG+ GEPG  G+AGV G KG +GK G  GP GP GVQG PG+ GPRGE G+KG IG  GP G PG +G AG PGK G  GA G PG+ G KG+ G  G RGEQG  G +GP G KG+KG  G+PGRQG  G++GP G+ GE G+Q         G PG  GE G  GLPG  G  G  G +G  G  G  G  G  G  G  G PG AG  GP G  G +   G  G  G+RGPPGI G  GQ GP GP G                 G  G PG  G +G  GK+G P   G KGE   KG+ G+PGT+G  G KGE G  G  GP GK+G  G  G+ G  GP GP G++GESG  GP+G  GPPG  G RG  G SGD G  G+ GP GQVKI N  E    RYKRS   Q   +   IN  +F +VD +  K++  R+ TG +  + ARTC D++L  K + +  Y+IDPNEGS +D+I  +C F ++  V+TC+ S   QG L  Y KP+  ++ WQ+ LR+  + +   DLH+ Y  H+Q+N LR Q+  + QE+EFLCD + IYG  +  + ++ F  AT L+ H+ + +DL+ GERLGP + +MDKR Y++   R   ++T+EYDGC Y T G+++K  +ET DL +LPIIDF++ +++ +   +L +NV ++C+
Sbjct:  822 KPGVEGIEGVQGEKGTKGAPGPSGPSGPVGLRGTRGPPGPSGDPGAKGDEGRRGPHGSLGDKGDRGPPGPHGPPGPIGPVGPQGLPGSQGEPGDKGARGPPGPVGETGEKGVQGLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGEIGNAGVPGPKGIEGKVGPPGPPGPAGVQGSPGRSGPRGEPGIKGVIGAEGPVGFPGPVGLAGAPGKMGVPGAPGSPGLPGLKGEQGKPGARGEQGAQGAIGPAGPKGMKGMLGRPGRQGQPGSAGPPGIVGEPGVQGAQGPMGPIGAPGLPGEPGSAGLPGKDGVRGDIGPEGIFGDDGPPGPPGPVGVPGVQGPPGTAGETGPIGDPGDRGAPGSIGSPGERGPPGIMGAEGQVGPQGPEG---------------EQGLQGPPGDSGPKGNIGKIGSPGPVGPKGESGPKGVGGNPGTVGPPGPKGEQGSIGDPGPSGKQGEMGGHGDQGPPGPRGPNGDEGESGPPGPIGQPGPPGLQGPRGEQGHSGDPGPMGKQGPQGQVKITNNGENLRMRYKRSAVVQTEETLEQINQNIFGRVDALNKKVRSRRYATGLSPQNPARTCKDVRLTNKFAQNDTYWIDPNEGSNRDAIRAKCTFYDDGTVETCVDSSMDQGILVTYLKPLPSDSEWQSQLRVMNSTTPL-DLHN-YGSHSQVNMLRIQHRYASQELEFLCDGTEIYGSWNPRTSQSDFSKATSLLAHNERMLDLTSGERLGPGKYHMDKRNYRNNLERVDTSITVEYDGCQYLTRGAATKLLIETRDLEVLPIIDFKVRNFDKQDTCSLSINVGAVCY 1653          

HSP 2 Score: 219.935 bits (559), Expect = 1.089e-53
Identity = 173/444 (38.96%), Postives = 235/444 (52.93%), Query Frame = 2
Query:  248 YRDGVVDLLTAVQISD-KNIGTTAEEPFCGNSRRK----AFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPF-EFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNR-TSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFN-DVMMNQGP--VKXXXXXXXXXXXXXXXXXXXXXXXXXNGRDGLPGTPGVPGAPGHIFVLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLT------------------------GPKGRSGDIGMQGLPGNKXXXXXXXXXXXXXXXXXXXXXXASGRPGDVGPPGE 1477
            ++D + DLL  V+I    +   T +  FC     +    A+ Y  G+ L   ++ +F   FP++FSIL T +     +Q +F + ++     LA++ G  I L YR  S  S   + F    + D +WHRIA S+   SV+LLIDC+ I S  LNR  ++ L  + G LYVLQD  G ASF+G ++ LL+V     A+ QC IY+P   +D   + M  Q P   ++    GQ G  G  GPKG KGQ G++GR G+PG PG+PG P HI ++P+P   +N   R+A FRT++++   +LKG+RG QGM GLPG QGP G RG KGD G  G+ GFIGP GLRG  G  GP GR G DGE G++GL                         GP G  GDIG++G PG                          GR G+VGPPGE
Sbjct:   54 FKD-ITDLLQLVEIDKLDSYEVTRQYGFCPTQLTENPNTAYSYHRGDKLKLPTEKVFPSSFPQDFSILTTIRLRHEQNQKIFDVIDETGQSQLAISVGNSIVLYYRL-SGDSLSIVEFTNVKMNDLRWHRIALSIKGDSVTLLIDCKVITSKLLNRPLTENLKFNKGVLYVLQDKTGQASFSGAIEQLLIVPSPGGAYDQCSIYSPCSESDKTIEEMQLQEPYIYEQTRNFGQPGEKGEVGPKGEKGQPGRDGRSGIPGAPGLPGVPSHILMMPLPSFGKNELSRMASFRTTVQQAVMMLKGSRGQQGMIGLPGIQGPPGPRGSKGDQGLIGEPGFIGPTGLRGPPGPVGPRGRPGVDGEVGASGLAGPKGLPGLPGLPGLPGHRGHKGEIGPSGIKGDIGLRGPPG------------------------PYGRDGEVGPPGE 471          

HSP 3 Score: 91.6633 bits (226), Expect = 1.767e-14
Identity = 166/389 (42.67%), Postives = 195/389 (50.13%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGV------------------SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG---------------------------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVG 3517
            RGPPG  G +G  G  G+ GK G  GP G  G +G  G+PGPQG +G RG+ G  G  G +G  G  GPQG  G+ GE       G  G  GP G  G  G PG  G  GV G+PG  GP GE G++G++G +G VG  GP G  G  G +G  G  G+ G  G+KG +GK G  G +G QG                    G+PGS G  G+ GE G QG QG  GP G  G+ G PG  G  G+ GV+GDIG                             G TG  G  G+ G PG  G+ G  G PG+ G +G  G  GP GEQGL GP G  G KG  G  G PG  G  G SGP+GV G  G  GPPG KGE G IG PGP G
Sbjct:  919 RGPPGPVGETGEKGVQGLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGE------IGNAGVPGPKGIEGKVGPPGPPGPAGVQGSPGRSGPRGEPGIKGVIGAEGPVGFPGPVGLAGAPGKMGVPGAPGSPGLPGLKGEQGKPGARGEQGAQGAIGPAGPKGMKGMLGRPGRQGQPGSAGPPGIVGEPGVQGAQGPMGPIGAPGLPGEPGSAGLPGKDGVRGDIGPEGIFGDDGPPGPPGPVGVPGVQGPPGTAGETGPIGDPGDRGAPGSIGSPGERGPPGIMGAEGQVGPQGPEGEQGLQGPPGDSGPKGNIGKIGSPGPVGPKGESGPKGVGGNPGTVGPPGPKGEQGSIGDPGPSG 1301          

HSP 4 Score: 81.6481 bits (200), Expect = 1.766e-11
Identity = 224/641 (34.95%), Postives = 270/641 (42.12%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGP---------------------TGELGSKGDRGKFGP---QGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGS---------------------VGPIGPDGEKGD---VGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ------------GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---------------AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGEL------------------------GIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE------RGDRGPPGIAGNMGQRGPN---GPIGIVXXXXXXXXX---------XXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE------PGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGPPG  G  G PG  G  G +G+ GPKG+ G  G+ G+ GP G  G +G  GP                      GE GSKG+RG  GP   +GP G KGE                                        G IGE G RGL G+KG+                      G IG  GEKGD   +GPLG +G  G RG  G +G+ G++G +G +G +            G  G  G+PG AGV          G  G  G  G  G+ G  G  GE G+ G+IG IG  G PG++GE                G PGK G  G EG  G KGTKG  G  GP G  GL       G++G  G  G PG +G  G  GP G  G+                         G QG PG KG  GP   PGPVG +G  G                       GL GK GL+GP G  G QG+ G       +GDRG PG  G+ GQ+G     GP G+VG  G  G           G +GP GP G  G +GS G  G  GEPG KG    +G  G PG +G  G+ G+      PG PGL G +G++G PG +GE G  G IGP G  G
Sbjct:  541 RGPPGVTGLPGSPGMTGNPGPQGNAGPKGEPGLTGEPGDTGPVGASGYKGDRGPRGLPGAKGAQGAAGLTGVRGDRGEKGSKGERGLPGPRGSEGPVGIKGE---------------------------------------PGIIGELGPRGLEGQKGNEGPRGSPGYPGGPGPKGTVGPAGGIGSPGEKGDPGHIGPLGLSGPVGPRGAPGPRGAPGQSGPVGFKGDEGPTGPTGPPGAAGPQGPRGTPGFAGVP---------GPPGLPGRPGPVGLLGDRGEPGEPGIPGEIGPIGMVGVPGQVGEQGAPGERGPPGPRGPQGIPGKPGVEGIEGVQGEKGTKGAPGPSGPSGPVGL------RGTRGPPGPSGDPGAKGDEGRRGPHGSLGDKGDRGPPGPHGPPGPIGPVGPQGLPGSQGEPGDKGARGP---PGPVGETGEKGVQ---------------------GLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGEIGNAGVPGPKGIEGKVGPPGPPGPAGVQGSPGRSGPRGEPGIKGVIGAEGPVGFPGPVGLAGAPGKMGVPGAPGSPGLPGLKGEQGKPGARGEQGAQGAIGPAGPKG 1103          

HSP 5 Score: 78.9518 bits (193), Expect = 1.358e-10
Identity = 227/614 (36.97%), Postives = 265/614 (43.16%), Query Frame = 2
Query: 2486 RGPPGTRGPSGL---PGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIR------------------------------------GARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLG------------KTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDA---GVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGIL---------GPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGL---PGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDR------GPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXS---------RGLDGKVGEPGQKGERYL---------------KGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGPPG  G  G    PG+PG  G +G +GP G  G RG  G  GP G++                                    GARG  G TG  GS G  G  GPQG  G                                                 KG  GL G  G  GP+G  G KGD GP G             TG++G RGE G KG  G  G  GS GP G+ GEPG  G+    G+ G+KG +G +GS G  G  G +G  G  G  G  G KGD G IGP G  G +G  G PG  GA G  G  G KG +G TG           GPRG  G                 G PG  G  G  GP G+ G+ G  G PG  GE+GPIG+   PG VG  G  G+ G PGP G  G  G  G  G  G+ G+ G +G  G SG  G  G RG R      G PG  G+ G+RGP+G +G  G  GP G  GP GP+GP+G  G  GS         RG  G VGE G+KG + L               +G +GSPG  GA G +GEPG  G TG QG  G+PGP+G  G  G IG  G  G
Sbjct:  454 RGPPGPYGRDGEVGPPGEPGSAGLQGPMGPVGPAGLRGLPGRSGPNGLKGEFGDSGEIGPPGPQGPVGPPGVPGPQGIRGLTGKPGARGPPGVTGLPGSPGMTGNPGPQGNAG------------------------------------------------PKGEPGLTGEPGDTGPVGASGYKGDRGPRGLPGAKGAQGAAGLTGVRGDRGEKGSKGERGLPGPRGSEGPVGIKGEPGIIGELGPRGLEGQKGNEGPRGSPGYPGGPGPKGTVGPAGGIGSPGEKGDPGHIGPLGLSGPVGPRGAPGPRGAPGQSGPVGFKGDEGPTGPTGPPGAAGPQGPRGTPGF---------------AGVPGPPGLPGRPGPVGLLGDRGEPGEPGIPGEIGPIGMVGVPGQVGEQGAPGERGPPGPRGPQGIPGKPGVEGIEGVQGEKGTKGAPGPSGPSGPVGLRGTRGPPGPSGDPGAKGDEGRRGPHGSLGDKGDRGPPGPHGPPGPIGPVGPQGLPGSQGEPGDKGARGPPGPVGETGEKGVQGLIGKPGLRGPTGADGEQGDQGSPGPQGAKGDRGEPGEQGSTGQQGAAGSPGPQGMVGEPGEIGNAGVPG 1004          

HSP 6 Score: 73.9442 bits (180), Expect = 4.306e-9
Identity = 138/294 (46.94%), Postives = 161/294 (54.76%), Query Frame = 2
Query: 2879 RKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGIL---------GPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRG 3724
             +G  G IGP G KGD+G  G  G  G  GEVG  G  G  GL G  GP G +G  G PG +G NG KGE G  G  GP GP G  G PG PGP+G +G+ G  G  GP   TG PG  G  G PG  G +G +G+PG+ G  GDTG +         GPRG  G  G  G  G  G++G RG+ G +G  G  GP+G EG +GI+G PG  GELGP GL G  G  GP G  G PG PG  G  G  G +GSPG  G  G  GP G SG  G  G  G RG PG +G +G +G
Sbjct:  435 HRGHKGEIGPSGIKGDIGLRGPPGPYGRDGEVGPPGEPGSAGLQGPMGPVGPAGLRGLPGRSGPNGLKGEFGDSGEIGPPGPQGPVGPPGVPGPQGIRGLTGKPGARGPPGVTGLPGSPGMTGNPGPQGNAGPKGEPGLTGEPGDTGPVGASGYKGDRGPRGLPGAKGAQGAAGLTGVRGDRGEKGSKGERGLPGPRGSEGPVGIKGEPGIIGELGPRGLEGQKGNEGPRGSPGYPGGPGPKGTVGPAGGIGSPGEKGDPGHIGPLGLSGPVGPRGAPGPRGAPGQSGPVGFKG 728          

HSP 7 Score: 64.6994 bits (156), Expect = 2.584e-6
Identity = 156/445 (35.06%), Postives = 187/445 (42.02%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLK---------GIKGSPGTIGAV---------------------------------------GSKGEP---GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGED 4036
            +G +G++G  G  GP GP G KGD G +G+ G  G             TGL G  GP G  G PG  G+ G +   G  G +G  G  G  G+ G  G  G  G  G+KGDIGL GP G  GR GE GPPG+ G++G +G  G  G  G  G+ G  G  GL G  G  G  G  G +G  G  G  G  G +G+ G+ G +GPPG        GLPG                              SPG+ G  G QG AG  G  G  GE GD GP G +G  G RGP G                    G  G  G  G  G+ G  GE G KGER L          GIKG PG IG +                                       G KG+P   GP GL+GP G RG PGP+G  G  GP+G  G++
Sbjct:  355 RGQQGMIGLPGIQGPPGPRGSKGDQGLIGEPGFIGP------------TGLRGPPGPVGPRGRPGVDGEVGAS---GLAGPKGLPGLPGLPGLPGHRGHKGEIGPSGIKGDIGLRGPPGPYGRDGEVGPPGEPGSAGLQGPMGPVGPAGLRGLPGRSGPNGLKGEFGDSGEIGPPGPQGPVGPPGVPGPQGIRGLTGKPGARGPPGV------TGLPG------------------------------SPGMTGNPGPQGNAGPKGEPGLTGEPGDTGPVGASGYKGDRGPRG------------------LPGAKGAQGAAGLTGVRGDRGEKGSKGERGLPGPRGSEGPVGIKGEPGIIGELGPRGLEGQKGNEGPRGSPGYPGGPGPKGTVGPAGGIGSPGEKGDPGHIGPLGLSGPVGPRGAPGPRGAPGQSGPVGFKGDE 730          

HSP 8 Score: 64.3142 bits (155), Expect = 3.163e-6
Identity = 49/103 (47.57%), Postives = 60/103 (58.25%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNK---GDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G RG  GS G+ G+ GP G +G  G+VG +G +GE G+QGP G  G  G  G IG  G  GP+G+ G K   G+ G+VGPPG  G  G  G  GP G QG  G
Sbjct: 1205 GDRGAPGSIGSPGERGPPGIMGAEGQVGPQGPEGEQGLQGPPGDSGPKGNIGKIGSPGPVGPKGESGPKGVGGNPGTVGPPGPKGEQGSIGDPGPSGKQGEMG 1307          
BLAST of Collagen type IV alpha 2 chain vs. TrEMBL
Match: A0A3R7GIF4 (Collagen alpha-1(XXVII) chain B OS=Clonorchis sinensis OX=79923 GN=col27a1b PE=4 SV=1)

HSP 1 Score: 367.851 bits (943), Expect = 3.311e-100
Identity = 386/895 (43.13%), Postives = 488/895 (54.53%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGE---AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ------------------GEAGERGD-------RGP---PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPP---------------GLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRST---RAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE--VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYG--DSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            RG  G RGP G PG+PG++G EG  G KGD+GP G +G  GP G+RG+ G  GP GE+G KGD+G+ G QG  GDKG+RG  G QGPPG  GP GP G+ GSPG+ G KG  G PGP G  GEKG+RGL+G+ G+ GP G DGE GD+G  G  G KG RG+ G +G  G TG +G+ G QG  GE G PG AGV G KG +GK G  GP GPVGVQG PG  GPRGE G K   GL GP G PG +G    +G PGK G  G  G PGV+G KG+ G+LG RG QG+                         G  GP G +G  GIQG PGR+G  GP G PG  G  G  G  G  GP G  G  G  G  G PG  G  G+ GP G  G                    G AGE G        +GP   PG  GN G+ G  GP+G                          G +GL G  G PG  G           G  G  GS G+PGPP               G  GP G  G PGP+G  G  GP G  G  G+                     PG  G+ G  GRPGP GQVK+ N PE    R +RST   R Q+  DIN  +F +VD +  + +  R P G      ARTC DI+L  K + +  Y+IDPNEG   D+I+V+CVF ++  V+TC+ S   QG L  Y KP+ G++ WQ+ LR+  + ++ P+LH  Y DH+Q+N LR Q+ ++ QE+EFLCD + IYG  DS+LSE  KF  AT L+ H+ + +DL  GERLG    +MDKR+Y++   R   ++T+EYDGC Y   G+++K ++ETTDL +LP+IDF++  ++  G+S+L LNV S+C+
Sbjct:  797 RGLAGPRGPQGNPGRPGVEGFEGPQGDKGDVGPIGPTGPSGPVGLRGSPGPVGPPGEVGPKGDQGRPGMQGSIGDKGDRGPHGPQGPPGVVGPTGPQGLPGSPGEPGSKGARGQPGPIGVRGEKGIRGLVGKPGAHGPTGVDGEMGDIGSPGPQGSKGDRGDRGEEGLPGPTGDIGATGLQGAPGESGEPGPAGVAGPKGIEGKAGPPGPEGPVGVQGSPGPNGPRGEPGTK---GLPGPDGHPGEVGPPGISGAPGKVGNPGPSGTPGVRGPKGEPGLLGSRGLQGI------------------------PGKPGPDGPQGPKGIQGRPGRQGRPGPAGPPGIAGHPGLQGIPGPQGPLGPPGDVGPQGSTGLPGRDGGRGIMGPDGAVGEDGPPGPPGPAGTPGPQGPVGSAGETGHVGETPGLQGPVGQPGTKGNPGKPGGTGPVG------------------------NPGDQGLKGVKGNPGTVGPT---------GPQGEQGSIGDPGPPGETGEVGGEGDVGPQGPRGPTGDDGEPGPQGRQGPAGPPGQKGPRGK---------------------PGQPGEPGPMGRPGPQGQVKVTNAPENGLRRVRRSTNPVREQTLEDINARIFQRVDTLYKRARLARHPQGTRIKYPARTCKDIRLVNKFAKNDTYWIDPNEGISGDTIKVQCVFYDDGRVETCVESTMDQGMLTTYMKPLAGDSEWQSQLRVMNS-TKPPNLHD-YGDHSQVNMLRIQHRRASQELEFLCDGTAIYGRWDSKLSEP-KFDVATALLAHNGRMLDLGSGERLGFGLEHMDKRIYRNTLDRIDTSITVEYDGCQYMNRGAATKIRVETTDLEVLPVIDFKVRSFDQSGLSSLSLNVGSVCY 1607          

HSP 2 Score: 215.312 bits (547), Expect = 3.117e-52
Identity = 148/374 (39.57%), Postives = 211/374 (56.42%), Query Frame = 2
Query:  233 TQALSYRDGVVDLLTAVQISDKNIGTTAEEP-FC---GNSRRKAFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRT-SDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNP-DCNNDFNDVMMNQGPVKXXXXXXXXXXXXXXXXXXXXXXXXXNGRD---GLPGTPGVPGAPGHIFVLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLTGPK 1327
            TQ+    +   DLL AV I          +P FC     ++  A+ Y  G  L  ++D +F   FP++FSIL T + +     ++F + +      LA+T G  ITL Y          + F   L   +W+RI+ S+   S++LL++C+ + S  L R+ SDP  +  G LYV+Q  +G ASFTG++D LL++     A+  C  ++P + +N F D M  +     E   G  G PG KG  G KG++G+ GRD   G+PG+PG PG P HI V+P+P   +N   R+A FRT ++++  +LKG+RG QGMTGLPG QGP GY G KG+ G  GD GF+GP GL+G  G  GP GR G DGE G++GL G K
Sbjct:   39 TQSQDSTEVAYDLLKAVTIDSAAPTDVIRQPGFCPPADGNQNTAYSYHRGGQLQVEADRVFPATFPRDFSILATVRLKTAAEGELFELVDPEGKRRLALTLGSTITLDYY--GEGQMRKVRFTQSLVPYRWYRISLSIKGDSITLLVNCRVVQSQMLVRSPSDPTGLESGTLYVMQHKDGKASFTGVVDRLLIIPTPEGAYNHCTTFSPCEKSNSFYDSMQAEEVETTEWQYGIPGAPGEKGEPGLKGEKGQPGRDGRSGIPGSPGSPGVPSHILVMPLPSFGKNELSRMASFRTMVQQSVMMLKGSRGQQGMTGLPGIQGPPGYVGEKGEQGHTGDPGFMGPSGLQGPPGPNGPRGRPGLDGEVGASGLPGSK 410          

HSP 3 Score: 102.834 bits (255), Expect = 6.337e-18
Identity = 195/554 (35.20%), Postives = 240/554 (43.32%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---GDVGPLGKTGLKGT---RGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGA------------------------------PGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RGPPG RGP G PG PG  G++G++G KG+ G  G  G+ GP G  G +G  GP G +G KGD+G  G  G  G++GE+G                                           KG  G  G +GS GP+G  GE    G+ GP G  G KG    RG  G  GS+G  G +G  GP G +GE G PG  G  G +G  G  G+ GP G  G  G  G  G  G  G  G IG +GP G+                              PG  G  G  G  G  GA GQ G +G  G+ G+ GPRG QG                           N G  GVE   G +GP G KG++GPIG  GP G  G  G  G  GPPG                  + G +G  G  G QG  G++GDRGP G  G  G  GP GP G+                   G+PG  GS+G  G+ G  G +GE+ ++G+ G PG  G  G  GE G  G  GPQG +G+ G +GE GL GP G +G  G
Sbjct:  530 RGPPGERGPQGSPGLPGNPGQQGNVGVKGEPGAPGLVGDSGPLGPPGYKGDRGPRGLIGPKGDQGAPGKTGVRGERGEKGA------------------------------------------KGEPGNAGPRGSDGPLGSKGENGMPGEPGPRGMEGPKGNQGGRGAAGFVGSQGPKGAVGPAGPIGRTGEKGDPGPIGPLGPRGPVGPPGAPGPRGAPGPSGPTGMKGDEGPVGPTGPIGPVGPQGSRGMPGFVGGAGPVGLPGRPGPPGYVGDRGEPGEPGLPGEIGPMGMVGAPGQTGEQGAVGERGLAGPRGPQG---------------------------NPGRPGVE---GFEGPQGDKGDVGPIGPTGPSGPVGLRGSPGPVGPPG------------------EVGPKGDQGRPGMQGSIGDKGDRGPHGPQGPPGVVGPTGPQGL------------------PGSPGEPGSKGARGQPGPIGVRGEKGIRGLVGKPGAHGPTGVDGEMGDIGSPGPQGSKGDRGDRGEEGLPGPTGDIGATG 975          

HSP 4 Score: 90.1225 bits (222), Expect = 5.609e-14
Identity = 81/142 (57.04%), Postives = 95/142 (66.90%), Query Frame = 2
Query: 1712 QGSAGLPGNPXXXXXXXXXXXXXXXXXXXXXXXXXXXXYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            QGS GLPGNPGQQG++G KG+ G  G  G+ G  GPPGYKG RG RG  G KGD G  G+ G RGE G KG KGE G  GPRG +G  G KG+ G+ GE GPRG +G KG++G  G  G+ G+ G KG+ GP G  GR GEK
Sbjct:  539 QGSPGLPGNPGQQGNVGVKGEPGAPGLVGDSGPLGPPGYKGDRGPRGLIGPKGDQGAPGKTGVRGERGEKGAKGEPGNAGPRGSDGPLGSKGENGMPGEPGPRGMEGPKGNQGGRGAAGFVGSQGPKGAVGPAGPIGRTGEK 680          

HSP 5 Score: 71.633 bits (174), Expect = 1.919e-8
Identity = 140/383 (36.55%), Postives = 166/383 (43.34%), Query Frame = 2
Query: 2921 GDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQG---VSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGN---------------MGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGP 4015
            G  GP G TGL G RG  G +G +G  GL G+ G QG   V GEPG+PG  G +G  G  G                         KG +G  GLIGP G  G  G+ G  G+ G  GA+G+PG  G +G  G LG +GE G+             G  G  G +G  GN G +G  G +G QGP G  G  GPIG  G  G  GP G  G  GP G  G  G  G  G  G  G  G +GP G +G  G  G +G RG PG  G                +G RG  G  G+                         G  G  G VG PGQ GE+   G +G  G  G  G+ G PG  G  GPQG +G+ GP G TG  GP
Sbjct:  516 GPQGPRGLTGLPGLRGPPGERGPQGSPGLPGNPGQQGNVGVKGEPGAPGLVGDSGPLGPPGY------------------------KGDRGPRGLIGPKGDQGAPGKTGVRGERGEKGAKGEPGNAGPRGSDGPLGSKGENGM------------PGEPGPRGMEGPKGNQGGRGAAGFVGSQGPKGAVGPAGPIGRTGEKGDPGPIGPLGPRGPVGPPGAPGPRGAPGPSGPTGMKGDEGPVGPTGPIGPVGPQGSRGMPGFVGGAGPVGLPGRPGPPGYVGDRGEPGEPGL------------------------PGEIGPMGMVGAPGQTGEQGAVGERGLAGPRGPQGNPGRPGVEGFEGPQGDKGDVGPIGPTGPSGP 838          

HSP 6 Score: 70.0922 bits (170), Expect = 6.038e-8
Identity = 144/392 (36.73%), Postives = 172/392 (43.88%), Query Frame = 2
Query: 2900 IGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ---GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGN---------------MGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            +GP G  G  GP G TGL G RG  G +G +G  GL G+ G Q   GV GEPG+PG  G +G  G  G                         KG +G  GLIGP G  G  G+ G  G+ G  GA+G+PG  G +G  G LG +GE G+             G  G  G +G  GN G +G  G +G QGP G  G  GPIG  G  G  GP G  G  GP G  G  G  G  G  G  G  G +GP G +G  G  G +G RG PG  G                +G RG  G  G+                         G  G  G VG PGQ GE+   G +G  G  G  G+ G PG  G  GPQG +G+ GP G TG  GP+G
Sbjct:  509 VGPPGMLGPQGPRGLTGLPGLRGPPGERGPQGSPGLPGNPGQQGNVGVKGEPGAPGLVGDSGPLGPPGY------------------------KGDRGPRGLIGPKGDQGAPGKTGVRGERGEKGAKGEPGNAGPRGSDGPLGSKGENGM------------PGEPGPRGMEGPKGNQGGRGAAGFVGSQGPKGAVGPAGPIGRTGEKGDPGPIGPLGPRGPVGPPGAPGPRGAPGPSGPTGMKGDEGPVGPTGPIGPVGPQGSRGMPGFVGGAGPVGLPGRPGPPGYVGDRGEPGEPGL------------------------PGEIGPMGMVGAPGQTGEQGAVGERGLAGPRGPQGNPGRPGVEGFEGPQGDKGDVGPIGPTGPSGPVG 840          

HSP 7 Score: 65.0846 bits (157), Expect = 1.850e-6
Identity = 152/484 (31.40%), Postives = 187/484 (38.64%), Query Frame = 2
Query: 2894 GPIGPDGEKGDVGP------------------------------------------LGKTGLKGTRGEV------------------GIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGD------TGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---------------------------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSR---GLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNP------GPKGETGLMGPIGPVGEDG 4039
            G +GP G KGD+GP                                          +G  G+KG  G+V                  G +G  G TGL G RGP G  G  GSPG  G                                   G +G++G+ G  GAPG +G++GP G  G  G  G  G+ G KGD      TG+ G RGE+G                       G  GN+GP+G +G LG +G  G  GE GP G+ GP G  G  G                       G  G  G +G  G +G  G  GE+GD GP                            G+ G+ G  GP GPIG VGP G RG PG +G  GP+G PG  G     G  G+ GEPG  GE    G+ G+PG  G  G+ GE G  G  GPQG  G P      GP+G+ G +GPIGP G  G
Sbjct:  429 GDVGPQGNKGDIGPRGPIGPRGRTGEVGPVGEPGPPGLQGPMGPPGPPGVRGLPGSMGPGGMKGEMGDVGIMGPPGPRGPVGPPGMLGPQGPRGLTGLPGLRGPPGERGPQGSPGLPG---------------------------------NPGQQGNVGVKGEPGAPGLVGDSGPLGPPGYKGDRGPRGLIGPKGDQGAPGKTGVRGERGEKGAK---------------------GEPGNAGPRGSDGPLGSKGENGMPGEPGPRGMEGPKGNQGGRGAA---------------------GFVGSQGPKGAVGPAGPIGRTGEKGDPGPIGPLGPRGPVGPPGAPGPRGAPGPSGPTGMKGDEGPVGPTGPIGPVGPQGSRGMPGFVGGAGPVGLPGRPGPPGYVGDRGEPGEPGLPGEIGPMGMVGAPGQTGEQGAVGERGLAGPRGPQGNPGRPGVEGFEGPQGDKGDVGPIGPTGPSG 837          
BLAST of Collagen type IV alpha 2 chain vs. TrEMBL
Match: A0A183ADB6 (Fibrillar collagen NC1 domain-containing protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS4951 PE=4 SV=1)

HSP 1 Score: 367.081 bits (941), Expect = 5.736e-100
Identity = 386/888 (43.47%), Postives = 498/888 (56.08%), Query Frame = 2
Query: 2528 KPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP------TGAPGRIGEAGPPGKTGASGAEGQPG------------------------VKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGP------------------------AGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRA---QSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE--VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSE-LSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            KPG++G EG  G KGD+GP G  G  GP G+RG  G +G  GE G KG++G+ G QG  GDKGERG PG QGPPG  GP GP G+ GSPG+ G KG  G PGP G  GEKGVRG  G++G+ GP+G DGE GD G  G  G KG RG++G +G  G  G +G+ GPQG  G+PG+ G AG                                G KGV+G  G +GP       G+PG  G  G PG  GA+GAEG PG                        +KG KGD    GP GE+GL                      G  G  GPQG +G  G +G PGR+G+ GP G PG +G  G  G  G  GPPG +G  G  G +G PG  G  G  GP                         G +G  G  G+ G+RGPPG  G++G+ G  G  G  GP GP G PG  GP G +G PGPKG+ G  G  G  G  GE  +KG+ G+PGT+G +G KGE G  G  GP GK+G  GP+GE G  GP GP G+DGE+G  G  GP GPPGPPG RG PG  GD G  GRPGP GQVK+ N PE    R +RS      Q+  ++N  +F +VD +  + +  R PTG ++   ARTC DI+L  K + +  Y+IDPNEG+  D+I  +C+F ++  V+TCI S    G L  Y KP+ G++ WQ+ LR+  + +  PDLH+ Y DH+Q+NFLR Q+  + QE EFLCD + IYG       E +F  AT LMTH+ + +DLS GERLG    +MD+RVY++   R    +T+EYDGC Y   G+S+K K+ET+DL +LPIIDF++  ++  G+S++ LNV ++C+
Sbjct:  756 KPGVEGFEGPQGEKGDVGPAGPPGPNGPVGLRGTAGPAGAPGEPGPKGEQGRAGTQGSIGDKGERGPPGPQGPPGVVGPAGPQGMPGSPGEPGSKGARGQPGPIGERGEKGVRGEAGKQGARGPVGADGEIGDQGSPGPQGTKGDRGDIGEQGPTGPQGDVGAAGPQGAPGQPGAVGPAGPK------------------------------GPKGVEGKAGPMGPEGPIGVQGSPGANGPRGEPGSKGATGAEGPPGLAGPVGTPGGPGKMGNPGPPGMPGLKGLKGDQ---GPPGERGLH---------------------GPQGEPGPQGPKGTKGNRGRPGRQGQHGPAGPPGVMGEPGHQGIPGPRGPPGPAGDMGPQGSIGPPGKDGFRGGIGPNGPFGEDGPPGPPGPAGTPGPQGPVGAAGEAGSVGDPGNRGPPGPVGSVGEPGLPGAAGPEGPVGPPGPPGEQGPQGNVGLPGPKGNVGKPGPTGPIGLPGEPGMKGVTGNPGTVGPIGPKGEQGSSGDPGPPGKQGERGPEGELGPPGPRGPAGDDGEAGPQGRQGPSGPPGPPGPRGKPGQVGDPGPMGRPGPQGQVKVTNAPEPGLRRRRRSASTYPEQTLEELNAKIFQRVDSLHKRARLARHPTGTSRQYPARTCKDIRLANKFARNDTYWIDPNEGANTDAINAKCIFYDDGRVETCIESEMDPGILATYVKPLPGDSEWQSQLRVLNSTT-TPDLHA-YGDHSQVNFLRIQHRSASQEFEFLCDGTAIYGKWNGKRNEPEFDAATALMTHNGRMLDLSTGERLGHGLEHMDRRVYRNSLSRLDTIITVEYDGCQYMNKGASTKLKVETSDLDVLPIIDFKVRSFDKHGLSSISLNVGAVCY 1587          

HSP 2 Score: 225.328 bits (573), Expect = 2.499e-55
Identity = 152/387 (39.28%), Postives = 214/387 (55.30%), Query Frame = 2
Query:  239 ALSYRD-GVVDLLTAVQISDKNIGTTAEEP-FC---GNSRRKAFRYESGNSLMAKSDVLFQGKFPKEFSILITAK-SEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNR-TSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFN--DVMMNQGPVKXXXXXXXXXXXXXXXXXXXXXXXXXNGRDGLPGTPGVPGAPGHIFVLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLTGPKGRSGDIGMQGLPGNK 1372
             L YR   V+DLL+AV IS  +    + +P FC   G     A+ Y  G +L   ++ +F   F  +FSILIT +  + L S+ +F + + N    LAV  G  I L Y    ST  +  P    L   +WHRI FSV   SV+LL+DC    SM L R  +  ++ + G LYVLQD  G  +FTG+++ LL++     A++ C  Y+P C+   N  D+M  +                 +  +      G++GR+G+PG+PG+PG   H+ ++P+P   +N   R+A FR+ ++++  +LKG RG QGMTGLPG QGP G  G KGD G  GD GF+GP GLRG  G  GP GR G DGE G++GL G KG+ G  G+ GLPG K
Sbjct:    3 TLVYRSQTVIDLLSAVSISSADPTEVSLQPGFCAPPGIVANTAYSYHRGGNLRVPAEKVFPDDFTSDFSILITMRLKQQLRSERLFELVDSNQKTRLAVDVGPSIKLIYGLDGSTQTLEFPAR--LVTHRWHRIGFSVKGDSVTLLLDCSVQQSMALRRPLTGRMNWANGTLYVLQDSTGKPAFTGVVERLLIIPSPEGAYEHCSTYSP-CDKMINIDDLMQLE----------------EETQEVRYANFGRDGRNGIPGSPGLPGVASHVLLMPLPSFGKNEMSRMASFRSMVQQSVMMLKGGRGQQGMTGLPGIQGPPGLVGEKGDQGVIGDPGFMGPTGLRGPPGPNGPRGRPGVDGEVGASGLPGSKGQPGMPGLPGLPGPK 370          

HSP 3 Score: 92.8189 bits (229), Expect = 7.069e-15
Identity = 83/143 (58.04%), Postives = 96/143 (67.13%), Query Frame = 2
Query: 1709 VQGSAGLPGNPXXXXXXXXXXXXXXXXXXXXXXXXXXXXYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            + GSAGLPGNPG QG +G KG+ G +GP G+ G  GP GYKG RG RG  GAKGD G  G+ G RG+ G KG KGE G  G RG +G  GVKG+ G+ GE GPRG +G KG++G  G  GY G  G KGS GPVG  GR GEK
Sbjct:  483 LPGSAGLPGNPGPQGSVGPKGEPGATGPIGDNGPMGPAGYKGDRGPRGPPGAKGDQGNPGKTGVRGDRGEKGSKGERGNPGARGSDGPIGVKGEPGMVGEPGPRGLEGPKGNQGPRGAAGYQGAQGPKGSVGPVGPIGRHGEK 625          

HSP 4 Score: 66.6254 bits (161), Expect = 6.767e-7
Identity = 72/193 (37.31%), Postives = 88/193 (45.60%), Query Frame = 2
Query: 2507 GPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGL------KGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAG 3067
            G +GLPG PG    +GS+GPKG+ G  G  G+ GP G  G +G  GP G  G+KGD+G  G  G  GD+GE+                                          G KG RG  G +GS GPIG  GE G VG  G  GL      +G RG  G +G++G  G +G  GP G  GE G PG  G
Sbjct:  485 GSAGLPGNPG---PQGSVGPKGEPGATGPIGDNGPMGPAGYKGDRGPRGPPGAKGDQGNPGKTGVRGDRGEK------------------------------------------GSKGERGNPGARGSDGPIGVKGEPGMVGEPGPRGLEGPKGNQGPRGAAGYQGAQGPKGSVGPVGPIGRHGEKGDPGMPG 632          

HSP 5 Score: 64.3142 bits (155), Expect = 3.219e-6
Identity = 63/148 (42.57%), Postives = 75/148 (50.68%), Query Frame = 2
Query: 3599 SPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            S GLPG  G QG  G  G  G  G  GD GP G AG  G RGP GP G           G  G  G  G  G +G +G  G+ G PG +G     G+KG PG +G  G +G  GP G  GP+G  G  G +G  G +GP+GP+G  GE
Sbjct:  486 SAGLPGNPGPQGSVGPKGEPGATGPIGDNGPMGPAGYKGDRGPRGPPGA---------KGDQGNPGKTGVRGDRGEKGSKGERGNPGARGSDGPIGVKGEPGMVGEPGPRGLEGPKGNQGPRGAAGYQGAQGPKGSVGPVGPIGRHGE 624          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Cavefish
Match: col27a1b (collagen, type XXVII, alpha 1b [Source:NCBI gene;Acc:560145])

HSP 1 Score: 137.502 bits (345), Expect = 2.705e-32
Identity = 282/905 (31.16%), Postives = 381/905 (42.10%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGE------LGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEG---KTGLMGSRGPQGVSG---EPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTK--------------------------------GDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE---------RGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGI------KGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDK-------VDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCFS 5014
            G +G  GLPG PGI G+ G  G  GD+GP GQ+G  GP+G  G  G  G  GE      +G  G+ G  G +G  G  GERG+PG QG P  +   G  G  G+ G  G +G  G PG +G  GE  ++G  G  G  G  GPDG KG+ G LG  G  G +GE+G+KG EG     GL+G RGP+G  G   E G PG+ G  G +G  G  G                     E G  G +G  GP G  G IG   PPGK G  G  G  G +G +                                GD G +G RG++G  G  G  G  G+ G RGK G  G  GN GP+G       QGP G KG+ GP G  G  G SG  G  G+ G  G  G  G +GP G PG+ G +G  GP G +G QG  GE          G  GPPG+ G+ G  GP G  G+ G  GP G                   RG+ G +G  G++G+  +KG       +G PG +G  G K   GPPG  GP G +G  GP+G  G  G IGPVG  G                                       Q   +N   G++ +    + +    D++  + D+       +  ++N I  I+ P G  +++ AR C D+    ++  DG Y+IDPN G   D+IEV C F+   +TC+  +                            +S+      M V   QMNF+   +S++ Q I   C    ++  +  S       +    T + + I            G +   + KDE            DG  ++T     +F+  T D ++LPI+D  +N    K  S   L V  +CFS
Sbjct:   39 GDKGIQGLPGMPGIRGRPGPQGFPGDIGPPGQNGPEGPKGEPGIMGNVGMLGERGLVGFIGPVGETGLLGEKGHPGTPGERGEPGPQGIPVSSAVIGETGEPGARGFPGIQGPSGPPGAKGIPGEP-IQGEPGHDGEAGRSGPDGAKGEKGDLGAEGEHGEKGEIGLKGKEGPPGDPGLVGVRGPEGKPGKIGERGKPGEKGSKGHQGHLGMTG---------------------EVGDLGPVGFPGPKGNRGTIGFVVPPGKMGMQGDPGLSGYEGHQGPQGPIGPPGPKGAKQGEQGDDGKIEGPAGPPGDIGPVGHRGDRGESGDPGYPGQIGVDGQRGKAGAPGLPGNPGPRGT------QGPKGSKGDQGPKGKKGVPGESGSRGSSGSVGLSGPKGVVGREGPEGLPGMDGLSGKDGPKGIAGEQGNDGEVGLPGNSGPHGQTGPPGLRGSQGSFGPKGERGLAGETGPIG------------------KRGVPGGMGNQGKQGDTGVKGQPGDPGEQGFPGILGIFGPK---GPPGDFGPSGIQGPKGPQGLMGREGTIGPVGIIGP--------------SGYPGPQGDKGNRGETGPPGPRGPQGPPKNGELGAAFQVIFDSYSALRSDMDLPMLDQGTEIFKTLHYLSNLIYSIKNPLG-TQENPARMCRDLLDCEQRMNDGTYWIDPNLGCSSDTIEVTCNFTGGGQTCLRPL---------------------------TVSKL----EMGVGLIQMNFIHLLSSEAVQLITIHCLNVPVWA-TAASRPPSASNSVRFKTWNGQMIQAG---------GLIQPDLVKDE--------CWMTDGRWHQT-----QFQFVTQDPNLLPIVDV-LNLPITKPGSNYHLEVGPVCFS 824          

HSP 2 Score: 96.2857 bits (238), Expect = 1.574e-19
Identity = 180/544 (33.09%), Postives = 221/544 (40.62%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDG------------------KEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSE---GKTGLMGSRGPQGVS---GEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNP---GPKGETGLMGPIGPVGEDG 4039
            GPPG  GP G  G+PGI G                  + G +G KG  G  G+ G PGPQGI  +    G TGE G++G  G  GP GP G KG  G+P                                           ++G  G  G  G  GPDG KG+ G LG  G  G +GE+G+KG E   G  GL+G RGP+G     GE G PG+ G                                  KG +G +G+ G  G  G +G  GP G  G  G    PG  G +GD G+ G  G Q                              G QG +G+  I+GP G  G++GP+G  G  G                          G PG PG+ G+ G  G +G  G  G  G RG  G  G+ G +GP G  G+ G  G RG  G +G  GP G  G +G  GL G  G  G+ G    KGI G  G  G VG  G  GP G TGP G RG+    GPKGE GL G  GP+G+ G
Sbjct:   66 GPPGQNGPEGPKGEPGIMGNVGMLGERGLVGFIGPVGETGLLGEKGHPGTPGERGEPGPQGIPVSSAVIGETGEPGARGFPGIQGPSGPPGAKGIPGEP-------------------------------------------IQGEPGHDGEAGRSGPDGAKGEKGDLGAEGEHGEKGEIGLKGKEGPPGDPGLVGVRGPEGKPGKIGERGKPGEKG---------------------------------SKGHQGHLGMTGEVGDLGPVGFPGPKGNRGTIGFVVPPGKMGMQGDPGLSGYEGHQ--------------GPQGPIGPPGPKGAKQGEQGDDGK--IEGPAGPPGDIGPVGHRGDRGE------------------------SGDPGYPGQIGVDGQRGKAGAPGLPGNPGPRGTQGPKGSKGDQGPKGKKGVPGESGSRGSSGSVGLSGPKGVVGREGPEGLPGMDGLSGKDGP---KGIAGEQGNDGEVGLPGNSGPHGQTGPPGLRGSQGSFGPKGERGLAGETGPIGKRG 490          

HSP 3 Score: 65.4698 bits (158), Expect = 4.745e-10
Identity = 110/343 (32.07%), Postives = 139/343 (40.52%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVG 3517
            GP G RG  G  G PG  G+ G  G +G  G  G  GNPGP+G +G +G         SKGD      QGP G KG                                G  G+ G  G +G  G +G++GR+G  G  G DG  G  GP G  G +G  GEVG+ G+   +G  G  GP G+ G  GS G  G                                 E+G+ G+ G IG  G PG +G  G  G TG  G  G PG +G  G  GI GP+G  G                           + GP G++G  G QG  GR+G +GP+G+ GP G
Sbjct:  331 GPVGHRGDRGESGDPGYPGQIGVDGQRGKAGAPGLPGNPGPRGTQGPKG---------SKGD------QGPKGKKG------------------------------VPGESGSRGSSGSVGLSGPKGVVGREGPEGLPGMDGLSGKDGPKGIAGEQGNDGEVGLPGN---SGPHGQTGPPGLRGSQGSFGPKG---------------------------------ERGLAGETGPIGKRGVPGGMGNQGKQGDTGVKGQPGDPGEQGFPGILGIFGPKGPPG---------------------------DFGPSGIQGPKGPQGLMGREGTIGPVGIIGPSG 565          

HSP 4 Score: 61.2326 bits (147), Expect = 9.906e-9
Identity = 108/293 (36.86%), Postives = 134/293 (45.73%), Query Frame = 2
Query: 3176 VKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAG---GSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGI--VXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
             +G++GL G  G PG  G+ G  G  G  G  G+PG +G  GD   +GP G+                              +GP+G +GE GI G  G  GE G +G  GPVG                    G  G  G PG PG+ G  GP G    S   GE GE G RG PGI      +GP+GP G   +  +  +G+PG  G  G  G  G KG +G  G  GE G+KGE  LKG +G PG  G VG +G  G PG  G +GK G  G KG  G +G  G VG+ G
Sbjct:   22 FEGEVGLPGEPGEPGFQGDKGIQGLPGMPGIRGRPGPQGFPGD---IGPPGQ------------------------------NGPEGPKGEPGIMGNVGMLGERGLVGFIGPVGE------------------TGLLGEKGHPGTPGERGEPGPQGIPVSSAVIGETGEPGARGFPGI------QGPSGPPGAKGIPGEPIQGEPGHDGEAGRSGPDGAKGEKGDLGAEGEHGEKGEIGLKGKEGPPGDPGLVGVRGPEGKPGKIGERGKPGEKGSKGHQGHLGMTGEVGDLG 257          

HSP 5 Score: 55.8398 bits (133), Expect = 4.863e-7
Identity = 42/94 (44.68%), Postives = 58/94 (61.70%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVG 2113
            GA+G +G  GA+G+HG  G++G +G+   +G  G+ G+ G RGPEG  G  G+ G  GE G +G  G+ G  G VG  G  G PG KG+RG +G
Sbjct:  182 GAKGEKGDLGAEGEHGEKGEIGLKGK---EGPPGDPGLVGVRGPEGKPGKIGERGKPGEKGSKGHQGHLGMTGEVGDLGPVGFPGPKGNRGTIG 272          

HSP 6 Score: 55.0694 bits (131), Expect = 8.368e-7
Identity = 45/102 (44.12%), Postives = 57/102 (55.88%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G RG  G +G  GP G  G  GE  ++G  G  G  G  GP+G  G KGD+G +GE G +G+ G KG EG  G PG  G  G +G  G +G +G+ GEK
Sbjct:  138 GEPGARGFPGIQGPSGPPGAKGIPGEP-IQGEPGHDGEAGRSGPDGAKGEKGDLGAEGEHGEKGEIGLKGKEGPPGDPGLVGVRGPEGKPGKIGERGKPGEK 238          

HSP 7 Score: 54.299 bits (129), Expect = 1.155e-6
Identity = 61/172 (35.47%), Postives = 74/172 (43.02%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSG------------NPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEV 2974
            PG  GP G  G PG+ G +GS GPKG+ G  G++G            N G QG  G +GQ G  GE G  G  G FGP+GP GD G                                         G  G KG +GL+GR+G++GP+G  G  G  GP G    KG RGE 
Sbjct:  450 PGNSGPHGQTGPPGLRGSQGSFGPKGERGLAGETGPIGKRGVPGGMGNQGKQGDTGVKGQPGDPGEQGFPGILGIFGPKGPPGDFGPS---------------------------------------GIQGPKGPQGLMGREGTIGPVGIIGPSGYPGPQGD---KGNRGET 579          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Cavefish
Match: col1a1a (collagen, type I, alpha 1a [Source:ZFIN;Acc:ZDB-GENE-030131-9102])

HSP 1 Score: 128.257 bits (321), Expect = 3.875e-29
Identity = 298/922 (32.32%), Postives = 375/922 (40.67%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPG---IDGKEGSIGPKGDLGPRG---QSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGP---QGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVG------------------PIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTG---ASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXX---------XXSPGLPGKAGLQGPAGGSGTQGEA------------------------GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGT---------IGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPV---GEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYK--RSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQ---QGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVD-HTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSK--FKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            PG +G +G PGKPG   + G+ G+ GP G  G RG   + G PGP G  G+RG  G  G  G+KG+ G  G    QGP G +G                                            GE+G  GL G KG  G                    G  GE G  GP+G TG +G  GE G  G+ G  G  G  G  G +G  G PGD G  G+ G  G  G+ G  GP G  G  G       KG +G  G  G TG PG  G  GPPG  G     GA G  G +G KG+ G  GP G  G  G  GP G+ G KG  G  G  GS+G  GPQG+ G+ GI G PG++GE G  GLPGP G  G  G  G  G  G  G  G  G           G+PG  G AG  G AG  G +GE                         G+RG+ GP G AG MG  GP GP G                             G  G  GE G+ GER +KG +G  G           G  G  G  GP G  GP G  G PG  G +GL GPIGP    G  GE G AGP GP GPPGPPG  G   D G               I  P E +   ++  R+  A    D +  +   +  ++ +I+ IR P G  K+  ARTC D+K+       G+Y+IDP++G  +D+I+V C   E  +TC+   +    Q   Y  K   + +  W      +G         S   D + Q+ FLR  ++++ Q I + C  S+ Y D         K A +L   +   I      R                     F  ++  DGC   T G+  K     +TT  S LPIID    D         G+ V  +CF
Sbjct:  630 PGPQGATGEPGKPGEQGLPGEAGATGPSGSRGDRGFPGERGAPGPAGPVGSRGSPGAAGNDGAKGESGASGAPGAQGPPGLQG------------------------------------------MPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQGDKGEPGAAGPVGPTGARGPPGERGESGAPGPAGFAGPPGADGQAGAKGEPGDTGAKGDAGAPGAAGATGAPGPQGPVGATGP------KGARGAAGPPGATGFPGAAGRVGPPGPAGNSGPPGAPGPSGKEGPKGNRGETGPAGRPGEIGAAGPPGAPGEKGTPGAEGAPGSSGIPGPQGIAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGPGGPGGERGPPGPMGPPGLAGPPGEPGREGTPGNEGSAGRDGAAGAKGDRGETGAAGAPGAPGPPGAPGPIGPAGKTGDRGESGPAGPAGAMGPAGPRGPSGPA---------------------------GARGDKGETGEAGERGMKGHRGFTGMQGPPGPPGPSGESGPAGSSGPAGPRGPSGSAGTPGKDGMSGLPGPIGPPGPRGRSGEIGPAGPPGPPGPPGPPGPSGGGFDIGF--------------IAQPQEKAPDPFRHFRADDANVMRDRDLEVDTTLKSLSQQIESIRSPDGTKKNP-ARTCRDLKMCHPDWKSGEYWIDPDQGCNQDAIKVYCNM-ETGETCVYPSQSDIPQKNWYTSKNIKEKKHVWFGEAMTDGFQFEYGGEESKPEDVNIQLTFLRLMSTEASQNITYHCKNSIAYMD---QASGNLKKALLLQGSNEIEIRAEGNSR---------------------FTYSVTEDGCTSHT-GAWGKTVIDYKTTKTSRLPIIDIAPMDVGAP-NQEFGIEVGPVCF 1434          

HSP 2 Score: 82.4185 bits (202), Expect = 3.833e-15
Identity = 219/554 (39.53%), Postives = 265/554 (47.83%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---------------PGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGD---KGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEV---------------GIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGA---EGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELG---PIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQ---KGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGP 4024
            G PG +G +G+ G+ G  G +G  GP G+ G RG  G                PG +G  GA G SGP G  G +G  G  GP+G TG+    GE G PGS+G  G  G  GP G  G+ G  G  G  G PGP G  G+ GV G  G KG+ G  G  GE+G +G +G  G  G  G+V               G +G  G  G  G  GPQG +GEPG PG+ G+             G  G  G  G  G  G  GE+G  G  G +G  G+PG  G  G  G++GASGA   +G PG++G  G+ G  G  G +G  G  G  G+ G  G  G  G QG  G  G  G  G  G +GPPG +GE G   P G  GP G  G +G  G PG  G  G AG  G  G+ G     G QGP G +G +G  G  G  G  G  G  G+ GP GP G           GP G  GP G  GPKG+RG  G  G PG+    G     G KG+PG  GA GS G PGP G+ G +G  G PG +GE G  G  GP
Sbjct:  421 GAPGAKGEAGVKGESGAPGVQGPPGPAGEEGKRGARGEPGAAGGRGPPGERGAPGARGFPGADGASGPKGAPGERGGPGAVGPKGATGEPGRNGEPGMPGSKGMTGSPGSPGPDGKTGATGPAGQDGRPGPPGPVGARGQPGVMGFPGPKGAAGEAGKPGERGVMGAIGVAGAPGKDGDVGAPGAPGAAGPSGERGEQGPAGPPGFQGLPGPQGATGEPGKPGEQGL------------PGEAGATGPSGSRGDRGFPGERGAPGPAGPVGSRGSPGAAGNDGAKGESGASGAPGAQGPPGLQGMPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQGDKGEPGAAGPVGPTGARGPPGERGESGAPGPAGFAGPPGADGQAGAKGEPGDTGAKGDAGAPGAAGATG---APGPQGPVGATGPKGARGAAGPPGATGFPGAAGRVGPPGPAG---------NSGPPGAPGPSGKEGPKGNRGETGPAGRPGEIGAAGPPGAPGEKGTPGAEGAPGSSGIPGPQGIAGQRGIVGLPGQRGERGFPGLPGP 950          

HSP 3 Score: 68.9366 bits (167), Expect = 5.489e-11
Identity = 196/516 (37.98%), Postives = 232/516 (44.96%), Query Frame = 2
Query: 2597 GNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---GDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRI---------------GEAGP---------PGKTGASGAEGQPGVKGTKGDTGILGP---RGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRK---GELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPG------------LTGPQGKRGNPGPKGETGLM 4009
            GN G  G  GA+G++G  GE G+ G +G  GP G  G +G RG+PG+ G  GP G  G                             G RG  G  G+ GP G  GE+   G VGP G TG  G  GE G+ GS+G TG  GS GP G +G  G  G  G  G  G  G +G  G  G  G +G  G+ G  GE+GV G IG+ G  G  G +               GE GP         PG  GA+G  G+PG +G  G+ G  GP   RG++G  G  G  G  G  G RG PG  G+ G  G  G  G  G QGPPG +   GE G  GLPG  G  G  G  GA G  G  G AG        G  G+ G  GP G +G +G  GERG+ G PG AG  G  G +G  G  G  G  G  G  G  G  G  G  G +   G VG  G KG R   G  G+ G  GA G  G PGP G              GP+G RG  GP G  G +
Sbjct:  415 GNTGEAGAPGAKGEAGVKGESGAPGVQGPPGPAGEEGKRGARGEPGAAGGRGPPGERGA---------------------------PGARGFPGADGASGPKGAPGERGGPGAVGPKGATGEPGRNGEPGMPGSKGMTGSPGSPGPDGKTGATGPAGQDGRPGPPGPVGARGQPGVMGFPGPKGAAGEAGKPGERGVMGAIGVAGAPGKDGDVGAPGAPGAAGPSGERGEQGPAGPPGFQGLPGPQGATGEPGKPGEQGLPGEAGATGPSGSRGDRGFPGERGAPGPAGPVGSRGSPGAAGNDGAKGESGASGAPGAQGPPGLQGMPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQ------GDKGEPGAAGPVGPTGARGPPGERGESGAPGPAGFAGPPGADGQAGAKGEPGDTGAKGDAGAPGAAGATGAPGPQ---GPVGATGPKGARGAAGPPGATGFPGAAGRVGPPGPAGNSGPPGAPGPSGKEGPKGNRGETGPAGRPGEI 894          

HSP 4 Score: 65.0846 bits (157), Expect = 6.895e-10
Identity = 151/393 (38.42%), Postives = 184/393 (46.82%), Query Frame = 2
Query: 2936 LGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGR------------------KGELGPIG------LPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
             G+ G  G +GE G+KG  G  G+ G  GP G  G+ G+ G+ G  G +G  G++G+ G  G  G  G  G  G  GE+G  G +G  G TG PGR GE G PG  G +G+ G PG  G  G TG  G  G  G  GP+G  G  G+ G  G  G  G  G  G +GV G +G+ G PG+                  +GE GP G      LPGP G +                           G PGK G QG  G +G  G +G RGDRG PG      +RG  GP G VG  G  G  G  G  G  G  G  G++G  G  G PG++G   L G+KG  G  GA G+ G  GPPG  G QG +G PG  G  G  G  GP GE GE
Sbjct:  417 TGEAGAPGAKGEAGVKGESGAPGVQGPPGPAGEEGKRGARGEPGAAGGRGPPGERGAPGARGFPGADGASGPKGAPGERGGPGAVGPKGATGEPGRNGEPGMPGSKGMTGSPGSPGPDGKTGATGPAGQDGRPGPPGPVGARGQPGVMGFPGPKGAAGEAGKPGERGVMGAIGVAGAPGKDGDVGAPGAPGAAGPSGERGEQGPAGPPGFQGLPGPQGAT---------------------------GEPGKPGEQGLPGEAGATGPSGSRGDRGFPG------ERGAPGPAGPVGSRGSPGAAGNDGAKGESGASGAPGAQGPPGLQGMPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQGDKGEPGAAGPVGPTGARGPPGERGE 776          

HSP 5 Score: 62.003 bits (149), Expect = 5.974e-9
Identity = 217/591 (36.72%), Postives = 256/591 (43.32%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGD------------------------LGPRGQSGNPGPQGIRGARGQSGPTGELG------------SKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPG---------------LPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAV------------------------GSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            PG++G +G PG PG DGK G+ GP G                          G  G++G PG +G+ GA G +G  G+ G              G+RG+ GP GP G +G                 GP G  G PG  G +G+ G  G  GP G +G RG  G +G+ GP GP G +G  G  G  G KG  G  G  G++G  GL G  G +G +G PG  GD G  G KG  G  G  GP G  G            +KG  G  G +GPTGA       GPPG+ G SGA G  G  G  G  G  G +GE G  G  G  G+ G  G  G PG QG  G +GP+G  G  G  G  G  G  G +G PGP G SGP G  G  G  G  G  G+ GP G PG                PG  G  G +G  G QG AG+RG  G PG  G  G  G  GP G  G  GP G  G  GP GP+G PG  G  G  G+ G PG +G     G  G+ G  G                          G +GE GP G  G  G  G  GP G  G  G  G  GE GE
Sbjct:  519 PGSKGMTGSPGSPGPDGKTGATGPAGQDGRPGPPGPVGARGQPGVMGFPGPKGAAGEAGKPGERGVMGAIGVAGAPGKDGDVGAPGAPGAAGPSGERGEQGPAGPPGFQG---------------LPGPQGATGEPGKPGEQGLPGEAGATGPSGSRGDRGFPGERGAPGPAGPVGSRGSPGAAGNDGAKGESGASGAPGAQGPPGLQGMPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQG------------DKGEPGAAGPVGPTGA------RGPPGERGESGAPGPAGFAGPPGADGQAGAKGEPGDTGAKGDAGAPGAAGATGAPGPQGPVGATGPKGARGAAGPPGATGFPGAAGRVGPPGPAGNSGPPGAPGPSGKEGPKGNRGETGPAGRPGEIGAAGPPGAPGEKGTPGAEGAPGSSGIPGPQGIAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGPGGPGGERGPPGPMGPPGLAGPPGEPGREGTPGNEGSAGRDGAAGAKGDRGETGAAGAPGAPGPPGAPGPIGPAGKTGDRGESGPAGPAGAMGPAGPRGPSGPAGARGDKGETGEAGE 1076          

HSP 6 Score: 62.003 bits (149), Expect = 6.779e-9
Identity = 201/566 (35.51%), Postives = 233/566 (41.17%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPK---GDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVG------------------PIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTG---ASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXX---------XXSPGLPGKAGLQGPAGGSGTQGE------------------------AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGT---------IGAVGSKGEPGPPGLTGPQGKRGNPG 3985
            +GP G  G  GLPG  G  G+ G  G +   G+ G  G SG+ G +G  G RG  GP G +GS+G  G  G  G  G+ G                             G  G  G PG QG  GE+G  GL G KG  G                    G  GE G  GP+G TG +G  GE G  G+ G  G  G  G  G +G  G PGD G  G+ G  G  G+ G  GP G  G  G       KG +G  G  G TG PG  G  GPPG  G     GA G  G +G KG+ G  GP G  G  G  GP G+ G KG  G  G  GS+G  GPQG+ G+ GI G PG++GE G  GLPGP G  G  G  G  G  G  G  G  G           G+PG  G AG  G AG  G +GE                         G+RG+ GP G AG MG  GP GP G                             G  G  GE G+ GER +KG +G  G           G  G  G  GP G  GP G  G PG
Sbjct:  618 QGPAGPPGFQGLPGPQGATGEPGKPGEQGLPGEAGATGPSGSRGDRGFPGERGAPGPAGPVGSRGSPGAAGNDGAKGESG---------------------------ASGAPGAQGPPGLQGMPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQGDKGEPGAAGPVGPTGARGPPGERGESGAPGPAGFAGPPGADGQAGAKGEPGDTGAKGDAGAPGAAGATGAPGPQGPVGATGP------KGARGAAGPPGATGFPGAAGRVGPPGPAGNSGPPGAPGPSGKEGPKGNRGETGPAGRPGEIGAAGPPGAPGEKGTPGAEGAPGSSGIPGPQGIAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGPGGPGGERGPPGPMGPPGLAGPPGEPGREGTPGNEGSAGRDGAAGAKGDRGETGAAGAPGAPGPPGAPGPIGPAGKTGDRGESGPAGPAGAMGPAGPRGPSGPA---------------------------GARGDKGETGEAGERGMKGHRGFTGMQGPPGPPGPSGESGPAGSSGPAGPRGPSGSAGTPG 1123          

HSP 7 Score: 60.8474 bits (146), Expect = 1.388e-8
Identity = 216/633 (34.12%), Postives = 255/633 (40.28%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIG------PKGDLGPRGQSGNPG------------PQGIRGARGQSGPTG------------------------------------ELGSKGDRGKFGPQGP---------------TGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------------VGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEG---------------QPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPG---------------TIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGP---IGPVGEDGE 4042
            RGP G +G  G PG PG+ G +G  G       KGD GP G  G PG            P+G+ G RG++GP G                                    E+G++G RG  GPQG                 G+ G  G PG +G PG  G  G  G  G  G  G  G  G PGP+G  GE G  G  G  G  G  G  G +G  GP G+ G +G RGE             G  G+ G  G  G+ GP+G  GE G PG  G                             G  GE G  G+ G+ G  G  G  G  GP GKTGA+G  G               QPGV G  G  G  G  G+ G  G +G IG  G  G  G  G  G+ G +GP G  GE G  GPPG +      GLPGP G +                           G PGK G QG  G +G  G +G RGDRG P      G+RG  GP G VG  G  G  G  G  G  G  G  G++G  G  G PG++G   L G+KG  G                 GA G KGEPG  G  GP G RG PG +GE+G  GP    GP G DG+
Sbjct:  228 RGPSGPQGARGFPGTPGLPGIKGHRGFSGLDGAKGDTGPAGPKGEPGAPGENGTPGAMGPRGLPGERGRAGPNGAAGARGNDGAAGAAGPPGPTGPAGPPGFPGGPGAKGEVGAQGGRGAEGPQGARGEPGNPGPAGPAGPAGNNGADGAPGLKGGPGAPGIAGAPGFPGPRGPPGPSGTPGAPGPKGNTGEAGAPGAKGEAGVKGESGAPGVQGPPGPAGEEGKRGARGEPGAAGGRGPPGERGAPGARGFPGADGASGPKGAPGERGGPGAVGPK---------------------------GATGEPGRNGEPGMPGSKGMTGSPGSPGPDGKTGATGPAGQDGRPGPPGPVGARGQPGVMGFPGPKGAAGEAGKPGERGVMGAIGVAGAPGKDGDVGAPGAPGAAGPSGERGEQGPAGPPGFQ------GLPGPQGAT---------------------------GEPGKPGEQGLPGEAGATGPSGSRGDRGFP------GERGAPGPAGPVGSRGSPGAAGNDGAKGESGASGAPGAQGPPGLQGMPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQGDKGEPGAAGPVGPTGARGPPGERGESGAPGPAGFAGPPGADGQ 794          

HSP 8 Score: 55.8398 bits (133), Expect = 4.596e-7
Identity = 122/310 (39.35%), Postives = 154/310 (49.68%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPK------GDLGPRGQSGNPGPQ------------------GIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEK---------GDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGAS---GAEGQPGVKGTKGDT 3307
            RG PG  GP G  G+PG +G+ G  G K      G  GP G++G  GP                   G+ G  G  G  GE G  G+RG  G  G  G  G+ GD G+ G PG AGP+G  G  G  G  G +G+ G  G  G  G+ G +GL G  G+ GP G  G++         G  GP+G  G  G  G  G KG  G +G  G++GP G+ G PG  G AG+ G KG++G QG++G  G  G  G  G  G +GE G  G +G  G  G PG  GE+G PG  G +   GA+GQ G KG  GDT
Sbjct:  495 RGGPGAVGPKGATGEPGRNGEPGMPGSKGMTGSPGSPGPDGKTGATGPAGQDGRPGPPGPVGARGQPGVMGFPGPKGAAGEAGKPGERGVMGAIGVAGAPGKDGDVGAPGAPGAAGPSGERGEQGPAGPPGFQGLPGPQGATGEPGKPGEQGLPGEAGATGPSGSRGDRGFPGERGAPGPAGPVGSRGSPGAAGNDGAKGESGASGAPGAQGPPGLQGMPGERGAAGLPGLKGDRGDQGAKGADGAAGPPGPAGAQGDKGEPGAAGPVGPTGARGPPGERGESGAPGPAGFAGPPGADGQAGAKGEPGDT 804          

HSP 9 Score: 53.9138 bits (128), Expect = 1.712e-6
Identity = 42/93 (45.16%), Postives = 50/93 (53.76%), Query Frame = 2
Query: 1859 GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +G  GP G+ G  GE G  G  GE G  GP+G  G  G  G  GI+G  G  G DG KGD G  GP G  G PG  G+ G +G +G  GE+
Sbjct:  202 GPRGAAGPPGKNGEDGESGKPGRPGERGPSGPQGARGFPGTPGLPGIKGHRGFSGLDGAKGDTGPAGPKGEPGAPGENGTPGAMGPRGLPGER 294          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Cavefish
Match: col11a2 (collagen, type XI, alpha 2 [Source:ZFIN;Acc:ZDB-GENE-000208-23])

HSP 1 Score: 123.635 bits (309), Expect = 1.253e-27
Identity = 117/406 (28.82%), Postives = 184/406 (45.32%), Query Frame = 2
Query: 3833 LDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGS--------SGRYKRSTRAQSFVDINDAL---FDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDY--NNKGISTLGLNVLSMCF 5011
            L G+ G  G+KG   L G+ G PG  G  G +G PGP G +G +G+ G PG    TG +GP GP G  G  G  G  G  G  GP G++G+ G  G  G  G    P  ++I    + S         G+    T    F++ N+ +   F  ++ +  +I+ +RFP G  KDS ARTC D+ L + + TD +Y+IDPN+G  +DS +V C F+   +TC+   K+   +     P +    W +       +S   D +   V   Q+ FLR  + ++ Q + + C  SV + DS  +  N ++ A   +  + + I               +   Y          +    DGC YR     +  ++ T  +  LP++D +I D+  NN+     G  V  +CF
Sbjct: 1420 LRGEAGAKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGSKGETGMPG---GTGPLGPAGPAGVPGPRGIKGAKGAAGGAGPKGEKGVQGPPGPPGPPGDVIQPLPIQIPKKSKRSIDASEILPDGKAMDDTTGTEFLNGNEGMEEVFGSLNSLRQEIEAMRFPLG-TKDSPARTCQDLLLSQPEYTD-EYWIDPNQGCTRDSFKVYCNFTAGGETCLYPSKEVENVKMNSWPKEKPGTWYSRFAKGSKMSYV-DSNGEPVGVVQIGFLRLLSLQARQNLTYHCHHSVAWADS--TSGNGYQRALHFLGSNEEEISY-------------ETNPY----------IKALSDGCSYRKGYDRTVLEINTPQVEQLPLLDMKITDFGENNQ---KFGFEVGPVCF 1791          

HSP 2 Score: 100.523 bits (249), Expect = 1.218e-20
Identity = 63/217 (29.03%), Postives = 98/217 (45.16%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGNSRR-----KAFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEF------DLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMN 880
            VD+L  +Q++    G      FC  SRR      A+R      + A +  LF  +FP+ FSI+   K++      + SI N+     L +  GR     Y D    S M  P ++      +L DG WHRIA SV K +V+LL+DC+   +  L R++ P+  + G          +  F G +  LL+      A+  C+ Y+PDC++    V   
Sbjct:   37 VDVLRVMQLNSLPEGVQKVPGFC-TSRRAGTADHAYRITKKAQISAPTKQLFS-RFPENFSIMALVKAKPGLQAFLLSIYNEQGVQQLGLELGRSPVFLYED---QSRMPAPEDYPLFTGINLADG-WHRIAISVQKKNVTLLLDCKKRMTKALPRSNKPVLDTKGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKVQAQ 247          

HSP 3 Score: 81.2629 bits (199), Expect = 8.788e-15
Identity = 179/511 (35.03%), Postives = 214/511 (41.88%), Query Frame = 2
Query: 2516 GLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQ------------------GIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG 3994
            G PG  G  G+ G +G  G  G +G+SG+PG Q                  G  GA G  G  GE G KGDRG  G  G  GDK                                                G RG  G  G VGP G DGE+GD G +G  GL G  G  G+ G +G  G+ GS   QGV G  G PG  G  G +GE G  G QG  G  G+ G  G  GP GEKG  G  GL    G PG  G  G PGK G SG +G  G  G +G  G  GPRG +G+ G  G  G                  + G +G +G  GI+G  G KGE G +G+PGP G                             G  G  G  GP G  G+ G  GE+G  G PG+ G  G++GP G +G                 G  G  G KG RGL GK G  GQ+G    +G +G  G+ G  G+KG  G  G  GP G+RG PGP+G
Sbjct:  507 GPPGPMGFTGRPGPLGTPGSNGLKGESGDPGAQGPRGPQGMPGPPGKAGRRGRAGADGARGMPGETGIKGDRGFDGLPGLPGDK------------------------------------------------GYRGNNGAMGPVGPPGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIAGS---QGVRGSDGPPGPKGNLGPQGEPGPPGQQGGPGTQGMPGPQGATGPPGEKGPTGKPGL---PGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGSIGYPGPRGIKGVQGIRGLKG------------------HKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGED---------------------------GPEGPKGRVGPPGEIGSLGPVGEKGKLGVPGLPGYPGRQGPKGSLGF---------------PGFPGTNGEKGGRGLIGKTGPRGQRGPTGPRGQRGPRGSTGKSGAKGSSGGDGPAGPPGERGLPGPQG 903          

HSP 4 Score: 75.8702 bits (185), Expect = 4.312e-13
Identity = 145/369 (39.30%), Postives = 178/369 (48.24%), Query Frame = 2
Query: 2942 KTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQ---GPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            +  LKG  G +G  G  G  G  GS G +G SG+PG+       G +G QG  G  G  G  G  G  G  G  GE G+KGD G     G PG  G+ G  G  GA G  G PG  G +GD G +GPRG  G                  +PG +G  G  GP G+ G  G++   GPPG KG LGP G PGP G  G  G  G PGP G +G  G+ GP G PGLPG  G  GP G  G +G +G +G++GP G  G++G  GP G  G+                           +G+ G  G  G+KGE    GIKG  G  G  G  G PGP G  GP+G +G  GP GE G +GP+G  G+ G
Sbjct:  502 RLALKGPPGPMGFTGRPGPLGTPGSNGLKGESGDPGA------QGPRGPQGMPGPPGKAGRRGRAGADGARGMPGETGIKGDRGF---DGLPGLPGDKGYRGNNGAMGPVGPPGEDGERGDDGDVGPRGLPG------------------EPGPRGLLGPKGPPGIAGSQGVRGSDGPPGPKGNLGPQGEPGPPGQQGGPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGSIGYPGPRGIKGV---------------------------QGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGSLGPVGEKGKLG 816          

HSP 5 Score: 70.8626 bits (172), Expect = 1.423e-11
Identity = 152/425 (35.76%), Postives = 180/425 (42.35%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIR---GARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGG---SGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG 3742
            RG  G  G  G+PG+ GI         KGD G  G  G PG +G R   GA G  GP GE G +GD G  GP+G  G+ G RG  G +GPPG AG  G  G  G PG KG  G  G PGP G  G  G +G+ G +G+    GP GEKG  G  G  G+ G  G  G  G EG +G  G++GP G  G  G PG  G+                                 KGV+G  GL          G  G  G+ G  G +G  GVKG +G+ G+ GPRGE                               GP+G +G +   GPPG  G LGP+G  G +G                            PGLPG  G QGP G     G  G  GE+G RG  G  G  GQRGP GP G
Sbjct:  557 RGRAGADGARGMPGETGI---------KGDRGFDGLPGLPGDKGYRGNNGAMGPVGPPGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIAGSQGVRGSDGPPGPKGNLGPQGEPGPPGQQGGPGTQGMPGPQGAT---GPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGSIGYPGPRGI---------------------------------KGVQGIRGL---------KGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGE------------------------------DGPEGPKGRV---GPPGEIGSLGPVGEKGKLGV---------------------------PGLPGYPGRQGPKGSLGFPGFPGTNGEKGGRGLIGKTGPRGQRGPTGPRG 867          

HSP 6 Score: 70.0922 bits (170), Expect = 2.243e-11
Identity = 51/103 (49.51%), Postives = 61/103 (59.22%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG +GIRG  G KG+ G  G  G +G+ G+KG +GE G+ GPRG +G  G KG +G  GE G  G  G KG  G  G PGY G  G KGS G  G  G +GEK
Sbjct:  743 KGVQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGSLGPVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGTNGEK 845          

HSP 7 Score: 68.1662 bits (165), Expect = 9.391e-11
Identity = 100/287 (34.84%), Postives = 119/287 (41.46%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLK---GTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGL 3340
            GPPG +GP+G PG PG+ G +G  G  G  GP G  GN GP G +G+ G  GP G  G +G RG  G +G  G+ G                                            G KG  G+ G +G VG  GP GE G  GP G+ G     G+ G VG KG  G  GL G  G QG  G  G PG  G N                                 G KG  GLIG TG  G+ G  GP G+ G  G+ G+ G KG+ G  G  GP GE+GL
Sbjct:  687 GPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGSIGYPGPRGIKGVQGIRGLKGHKGEKGEDG------------------------------------------FPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGSLGPVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGTN---------------------------------GEKGGRGLIGKTGPRGQRGPTGPRGQRGPRGSTGKSGAKGSSGGDGPAGPPGERGL 898          

HSP 8 Score: 65.855 bits (159), Expect = 4.242e-10
Identity = 48/106 (45.28%), Postives = 63/106 (59.43%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGI------QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G  G  G SG KG+ GPSG  G+ G  G +G+KG  GI      +G +G +G  G+KGD G++GE G  G  G +G++G  GP G  G PG  GS GPVG +G+ G
Sbjct:  711 GHPGKEGPSGTKGNQGPSGPQGSIGYPGPRGIKGVQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGEDGPEGPKGRVGPPGEIGSLGPVGEKGKLG 816          

HSP 9 Score: 64.3142 bits (155), Expect = 1.439e-9
Identity = 128/350 (36.57%), Postives = 154/350 (44.00%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRG---LLGRKGSVGPIG------PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGT 3520
            G RG +G  G  G  G++G  G  GD+GPRG  G PGP+G+ G +   GP G  GS+G RG  GP GP G+                GP G  G  G  G  G +G+ G  G  GP GEKG  G   L G  G+ GP G      P G KG+ GP G  G  G  G  GIKG +G  GL G +G +G  G PG  GD GV                            G RGE GV G  G  GP G  GR+G  G  G  G  G +G+ GV G  G  G  GP+G  G                       G  G +G +G  G +G  GP G++G  GP G  GP G+
Sbjct:  591 GYRGNNGAMGPVGPPGEDGERGDDGDVGPRGLPGEPGPRGLLGPK---GPPGIAGSQGVRGSDGPPGPKGN---------------LGPQGEPGPPGQQGGPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGSIGYPGPRGIKGVQGIRGLKGHKGEKGEDGFPGIKGDFGVK---------------------------GERGEVGVPGPRGEDGPEGPKGRVGPPGEIGSLGPVGEKGKLGVPGLPGYPGRQGPKGSLGF---------------------PGFPGTNGEKGGRGLIGKTGPRGQRGPTGPRGQRGPRGS 874          

HSP 10 Score: 55.4546 bits (132), Expect = 7.636e-7
Identity = 46/100 (46.00%), Postives = 57/100 (57.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSV---GPPGYAGNPGIKGSRGPVGNQG 2122
            GARG+ G +G KGD G  G  G  G+   KG +G  G  GP GP G  G +GD    G+ GPRG  G  G  G +   GPPG AG+ G++GS GP G +G
Sbjct:  564 GARGMPGETGIKGDRGFDGLPGLPGD---KGYRGNNGAMGPVGPPGEDGERGD---DGDVGPRGLPGEPGPRGLLGPKGPPGIAGSQGVRGSDGPPGPKG 657          

HSP 11 Score: 54.6842 bits (130), Expect = 1.144e-6
Identity = 45/107 (42.06%), Postives = 59/107 (55.14%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRG---------EVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G +G++G  G+ GP+G+ G  G           G +G  G  G QGP GP+G  G  G  GI+G  G RG  G+KG++G  G PG  G+ G+KG RG VG  G  GE
Sbjct:  681 GPQGATGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGSIGYPGPRGIKGVQGIRGLKGHKGEKGEDGFPGIKGDFGVKGERGEVGVPGPRGE 787          

HSP 12 Score: 54.299 bits (129), Expect = 1.485e-6
Identity = 58/139 (41.73%), Postives = 74/139 (53.24%), Query Frame = 2
Query:  974 GRDGLPGTPGVPGAPGHIFVLPIPDSEENSNR------RVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLTGPKGRSGDIGMQGLPGNK 1372
            G+ GLPG  GVPG PG   +LP    +   ++      + AQ +  + +    LKG  GP G TG PG  G  G  G+KG+ G  G  G  GP+G+ G  G  G  GR+G DG RG  G TG KG  G  G+ GLPG+K
Sbjct:  452 GKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAQAILSQARLALKGPPGPMGFTGRPGPLGTPGSNGLKGESGDPGAQGPRGPQGMPGPPGKAGRRGRAGADGARGMPGETGIKGDRGFDGLPGLPGDK 590          

HSP 13 Score: 53.9138 bits (128), Expect = 1.849e-6
Identity = 39/91 (42.86%), Postives = 53/91 (58.24%), Query Frame = 2
Query: 1883 SGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGE------TGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G+ G  G  G +G+ GE GI+G RG +G+ G+ GD G +G        GP G+DG +GD+G VGP G  G PG +G  GP G  G  G +
Sbjct:  554 AGRRGRAGADGARGMPGETGIKGDRGFDGLPGLPGDKGYRGNNGAMGPVGPPGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIAGSQ 644          

HSP 14 Score: 52.7582 bits (125), Expect = 4.747e-6
Identity = 109/306 (35.62%), Postives = 129/306 (42.16%), Query Frame = 2
Query: 3218 GRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGP--RGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDR---GPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGE---PGQKGERYLKGIKGSPGTIGAVGSKGEPGPPG------------------------LTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G  GE GPPGK G  GA+G PG  GT     ++ P   G+ G D        +                       +  L ++GPP   G +G  G PGP+GT G +G  G  G PG  G  G  G  G PG  G+ G  G  G  G  GE G +GDR   G PG+ G+ G RG NG +G V                  G PG  G RG DG VG    PG+ G R L G KG PG  G+ G +G  GPPG                        + GPQG  G PG KG TG  G  G  G DG
Sbjct:  443 GDPGERGPPGKAGLPGADGVPGPPGTS----VMLPFRFGQSGGDKGPAVSAQEAQAQAILS---------------QARLALKGPP---GPMGFTGRPGPLGTPGSNGLKGESGDPGAQGPRGPQGMPGPPGKAGRRGRAGADGARGMPGETGIKGDRGFDGLPGLPGDKGYRGNNGAMGPV------------------GPPGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIAGSQGVRGSDGPPGPKGNLGPQGEPGPPGQQGGPGTQGMPGPQGATGPPGEKGPTGKPGLPGMPGADG 708          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Cavefish
Match: col1a2 (collagen, type I, alpha 2 [Source:ZFIN;Acc:ZDB-GENE-030131-8415])

HSP 1 Score: 118.627 bits (296), Expect = 3.889e-26
Identity = 291/918 (31.70%), Postives = 381/918 (41.50%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGN---PGPQGIRGARGQSGPTGELGSKGDRGKFGPQG---PTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLG---KTGLKGTR---------------GEVGIKGSEGKTGLMGSRGPQGVSGE------------------PGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPG---KTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG------------------------------ETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDH--TQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGC-MYRTPGSSSKFKLE--TTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            G +GPSG PG  G+ G  G +G  G  G RG  G+    GP G++G RG  GP G  G++G  G  GP G   P G KGE G  G+ G PG                                  +G  G+ G +G+ G  G  GEKG+ G  G     G  G R               G+ G  GS G +G  G+RGP G  GE                   G+ G+ G  G KG+ G  G  GP G  G  G  G  GP G +G  G  GL G  GA GR+G AGP G     G +GA+G+ G +G +GD G  GP+GE GL GP G  G KG  G  G  G  G    +GPQG  G  G  G PG +GE G   LPG  G+ G +G+ G  G PG  G +G+       GLPG  G QG AG  G+ G  G  G  G  GI G+ G+ G  GP+GI G  GP G  G +G  G  G  G   S G  G VG  G +G  +L+G  G  G  G  G KG+ G  GL G  G +G PGP G                               +G  GPIGP G  G  G  GP+GP G PG  G  G  G   D  G        Q  +R      +  Y+     +S     D L        N  +  R               DI+L     T   Y+IDPN+G   D+I+  C FS   +TCI +  +     N+ +  + + +      + G      +  ++      TQ+ F+R   +++ Q + + C  S+ Y D   +E    K A +L                   +G  D  +  +   R  F  ++  DGC + R  G  SK  +E  T   S LPI+D    D         GL++  +CF
Sbjct:  533 GEQGPSGAPGFQGLPGPAGPVGETGKPGDRGIPGDQGVAGPSGVKGERGTPGPAGASGAQGPIGARGPAGTPGPDGSKGEPGAVGAAGAPG---------------------------------HQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGRDGARGAPGPSGPPGPAGANGDKGETGSFGPSGPAGARGPSGERGESGPAGPPGFAGPPGADGQAGARGEKGPGGVKGDVGAPGPAGPAGNTGPLGPSGPVGPPGARGDSGPPGLTGFPGAAGRVGPAGPAGIVGPPGPTGAQGKDGPRGLRGDVGPAGPQGENGLIGPPGLSGEKGSPGESGPMGPPGI---AGPQGQLGSQGFNGLPGSRGERG---LPGAAGSVGEAGRVGPSGSPGPRGPSGNI------GLPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGPLGIAGAPGPLGPSGAVGRPGNRGEAG---SAGPSGAVGPAGARGAPFLQGPSGPRGEKGVAGDKGDRGMKGLRGHPGLQGMPGPNGPSGDTGAAGIAGPAGPRGPAGPNGPPGKDGRSGTHGPIGPAGHRGPPGHVGPVGPPGSPGLAGPPGAAGGGYDVSGGYDEYRADQAALR------AKDYEVDATIKSLNTQIDNLLSPEGSKKNPARTCR---------------DIRLSHPDWTS-FYWIDPNQGCTMDAIKAFCDFSTG-QTCIHAHPESIPRKNWYRSSQEKKHIWFGETINGGTEFGYNDETLSPQSMATQLAFMRLLANQAVQNVTYHCKNSIAYMD---AENGNLKKAVLL-------------------QGSNDVELRAEGNSR--FTFSVLEDGCTLQRHTGQWSKTVIEYRTNKPSRLPILDIAPLDIGGA-DQEFGLDIGPVCF 1354          

HSP 2 Score: 80.8777 bits (198), Expect = 1.166e-14
Identity = 198/541 (36.60%), Postives = 243/541 (44.92%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKG------DLGPRGQSGNPGPQGIRGARGQSGPT---GELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGP---------------DGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP---GKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G  GP GL G PG  G++G  G  G        GP G++G PG +GI G +G +GP+   GE G+ G  G  G QGP G +G  G PG  G  G  G  G  G  G  G  G  G  G  G  G  GEKG  G  G +G+ G  G                +G+KG+ G  G +G  G RG  G +G  G  G  G  GP G  G+ G+ G+ G  G KG+ G  G  GP G  G  G  G  GP G +G  G  GL G  GA GR+G AGP    G  G +GA+G+ G +G +GD G  GP+GE GL                            GP G+ GE   +G PG  G +GP G+ GP G  G  G +                     GLPG  G +G  G +G+ GEAG  G  G P      G RGP+G IG+                G  G  G  G  G  G  G PG+ G   +KG +G PG+ G +G  G PGP    GP G  G PG +GE G  GP G VG  G
Sbjct:  518 GPAGPVGLGGAPGEKGEQGPSGAPGFQGLPGPAGPVGETGKPGDRGIPGDQGVAGPSGVKGERGTPGPAGASGAQGPIGARGPAGTPGPDGSKGEPGAVGAAGAPGHQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGRDGARGAPGPSGPPGPAGANGDKGETGSFGPSGPAGARGPSGERGESGPAGPPGFAGPPGADGQAGARGEKGPGGVKGDVGAPGPAGPAGNTGPLGPSGPVGPPGARGDSGPPGLTGFPGAAGRVGPAGPAGIVGPPGPTGAQGKDGPRGLRGDVGPAGPQGENGL---------------------------IGPPGLSGE---KGSPGESGPMGPPGIAGPQGQLGSQGFN---------------------GLPGSRGERGLPGAAGSVGEAGRVGPSGSP------GPRGPSGNIGL---------------PGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGPLGIAGAPGP---LGPSGAVGRPGNRGEAGSAGPSGAVGPAG 983          

HSP 3 Score: 65.4698 bits (158), Expect = 5.794e-10
Identity = 181/525 (34.48%), Postives = 228/525 (43.43%), Query Frame = 2
Query: 2540 DGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQG---PTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGA---------EGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGP---------PGLTGPQG---KRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G  G +G  G  G +G+ G  G  G +G  G +GP GE G  GDRG  G QG   P+G KGERG PG  G                                      G +G +G +G  G  GPDG KG+ G +G  G  G +G  G+ G  G  G  G++G +G +G  G  G+AG +G +G  G  G  GP G                 G KG+ G  GP+G  G  G +G  G++G +G          +GQ G +G KG  G+   +G+ G  GP GP G+ G  G  G  G  G+ G+SGP G+ G  G  G  G  G  G +G PGP G  G  G  G  G  G +            G  G+ GL GP G SG +G  GE G  GPPGIAG  GQ G                                 S+G +G    PG +GER L G  GS G  G VG  G PGP         PG+TGPQG   + G+PG  G  G  G  G  G+ GE
Sbjct:  517 NGPAGPVGLGGAPGEKGEQGPSGAPGFQGLPGPAGPVGETGKPGDRGIPGDQGVAGPSGVKGERGTPGPAGA------------------------------------SGAQGPIGARGPAGTPGPDGSKGEPGAVGAAGAPGHQGAAGMPGERGAAGTPGAKGEKGEAGYRGLEGNAGRDGARGAPGPSGPPGPAG---------------ANGDKGETGSFGPSGPAGARGPSGERGESGPAGPPGFAGPPGADGQAGARGEKGPGGV---KGDVGAPGPAGPAGNTGPLGPSGPVGPPGARGDSGPPGLTGFPGAAGRVGPAGPAGIVGPPGPTGAQGKDGPRGLRGDVGPA------------GPQGENGLIGPPGLSGEKGSPGESGPMGPPGIAGPQGQLG---------------------------------SQGFNGL---PGSRGERGLPGAAGSVGEAGRVGPSGSPGPRGPSGNIGLPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGE 939          

HSP 4 Score: 59.3066 bits (142), Expect = 4.700e-8
Identity = 66/192 (34.38%), Postives = 82/192 (42.71%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGV 3070
            PG+RG  GLPG  G  G+ G +GP G  GPRG SGN G  G+ G +G++G  G  G+ G  G+ G  G  GD+G                                             E G  G LG  G+ GP+GP G  G  G  G+ G  G  G VG  G+ G   L G  GP+G  G  G  GD G+
Sbjct:  865 PGSRGERGLPGAAGSVGEAGRVGPSGSPGPRGPSGNIGLPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRG---------------------------------------------EPGSPGPLGIAGAPGPLGPSGAVGRPGNRGEAGSAGPSGAVGPAGARGAPFLQGPSGPRGEKGVAGDKGDRGM 1011          

HSP 5 Score: 56.9954 bits (136), Expect = 1.989e-7
Identity = 65/188 (34.57%), Postives = 78/188 (41.49%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGE---KGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGE 3043
            G PG RGPSG  G PG+ G +G  G +G  G  G  G PG  GI+G RG+ G  G LG  G  G  GP G  G  G R                                             G  G  G  G+VGP G  G    +G  GP G+ G+ G +G+ G+KG  G  GL G  GP G SG+
Sbjct:  890 GSPGPRGPSGNIGLPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPGPLGIAGAPGPLGPSGAVGRPGNR---------------------------------------------GEAGSAGPSGAVGPAGARGAPFLQGPSGPRGEKGVAGDKGDRGMKGLRGHPGLQGMPGPNGPSGD 1032          

HSP 6 Score: 55.8398 bits (133), Expect = 5.473e-7
Identity = 45/98 (45.92%), Postives = 55/98 (56.12%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            KG RG  GS G  G  G  G +G  G VG  G +GE G  GP G  G AG +G   +QG +GPRG+ G  GD+G  G  G  G+PG++G  GP G  G
Sbjct:  934 KGDRGEPGSPGPLGIAGAPGPLGPSGAVGRPGNRGEAGSAGPSGAVGPAGARGAPFLQGPSGPRGEKGVAGDKGDRGMKGLRGHPGLQGMPGPNGPSG 1031          

HSP 7 Score: 55.0694 bits (131), Expect = 9.311e-7
Identity = 45/97 (46.39%), Postives = 55/97 (56.70%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKG---EYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            GI G  G  G  G +G  G+RGE G+ G  G   E G  GP G  G  G  G+IG+ G TGP+G+ G +G  G+ GPPG  G  GIKG RG  G+ G
Sbjct:  848 GIAGPQGQLGSQGFNGLPGSRGERGLPGAAGSVGEAGRVGPSGSPGPRGPSGNIGLPGMTGPQGEAGREGSPGNDGPPGRPGAAGIKGDRGEPGSPG 944          

HSP 8 Score: 52.373 bits (124), Expect = 4.988e-6
Identity = 42/95 (44.21%), Postives = 52/95 (54.74%), Query Frame = 2
Query: 1871 DHGPSGQVG------TRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            ++GP+G VG       +GE G  G  G  G+ GP GP G  G  GD GI G+ G  G  G KG+ G+ GP G +G  G  G+RGP G  G DG K
Sbjct:  516 NNGPAGPVGLGGAPGEKGEQGPSGAPGFQGLPGPAGPVGETGKPGDRGIPGDQGVAGPSGVKGERGTPGPAGASGAQGPIGARGPAGTPGPDGSK 610          

HSP 9 Score: 51.9878 bits (123), Expect = 6.370e-6
Identity = 48/103 (46.60%), Postives = 56/103 (54.37%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGI---QGPRGPEGIAGVKGDIGIQGET---GPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            GA+G  G  G +GD GP+G  G  G +G  G+ GE G     GP GP GIAG +G +G QG     G RG+ G  G  GSVG  G  G  G  G RGP GN G
Sbjct:  800 GAQGKDGPRGLRGDVGPAGPQGENGLIGPPGLSGEKGSPGESGPMGPPGIAGPQGQLGSQGFNGLPGSRGERGLPGAAGSVGEAGRVGPSGSPGPRGPSGNIG 902          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Cavefish
Match: col5a3b (collagen, type V, alpha 3b [Source:ZFIN;Acc:ZDB-GENE-110728-4])

HSP 1 Score: 117.087 bits (292), Expect = 1.001e-25
Identity = 110/397 (27.71%), Postives = 172/397 (43.32%), Query Frame = 2
Query:  263 VDLLTAVQISDKNIGTTAEEPFCGN-----SRRKAFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGPVKXXXXXXXXXXXXXXXXXXXXXXXXXN------------GRDGLPGTPGVPGAPGHIFVLPIPDSEENSNR------RVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGD---PGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGER-GSNGLTGPKGRSGDIGMQGL 1360
            +D+L  + +SD+  G + E   C N         A++ +    +   +  LF  +FP+ FS+L+T +        + SI N +    L +  GR     Y D    PS   +     + +L DG+WHR+AFSV+   V+L +DC+ + ++ L R  +P   + G          +  F G +  L++    + A   C  Y PDC++      +   P +      +     P GP G  G  G              GR GL G  G+PG PG++ +LP      +  +      + AQ +  ++     +KG  GP G+ G PG  G  G  G+KGD   PG  GD GF             G PG  G  G R GS+G  GP+G+ G+ G +G+
Sbjct:    4 IDVLKILDLSDRMEGVSLEAGLCTNRPGLDEVDLAYKIDKKIQVSVPTKQLFPSEFPENFSVLVTVRPRRGAQCFLLSIYNPDGVQQLGLELGRSPVFLYEDQNGRPSPDQYPIF-KKINLADGRWHRLAFSVEGKKVTLYLDCKKLTTLDLPRGEEPKVSTEGVTVFGTRLLDEEVFEGEIQHLIISPDPAAAADYCVNYIPDCDSALLYSSLLLDPEEGPGGPEKGEKGEPAGPPGEAGPMGPPGPRGDPGDLGPAGRQGLAGADGIPGPPGNMLMLPQFQFGGDGQKVPVVSAQEAQAQAILQHAKLSMKGPPGPLGLNGRPGPLGNPGPTGLKGDSGLPGPPGDRGF------------DGLPGLPGNKGHRQGSDGQPGPRGQPGEPGGRGV 387          

HSP 2 Score: 104.76 bits (260), Expect = 5.739e-22
Identity = 212/543 (39.04%), Postives = 259/543 (47.70%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGI---RGARGQSGPTGELGSKGDRGKFG-------PQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGR----KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGER---GDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER--------YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            GPPG RG  GLPG PG  G     G  G  GPRGQ G PG +G+   +G++G  G  GE G+ G +G  G       PQG  G  G++G  G                                       +KG+RGL G     +G  G  GP GEKG  G  G  G  G  G  G+KG++G  G+ GS+G +G  G PG+ G+ GV G+ G+ G  G +G  GP G +G  G  G  G  G+ G+ G +G  G PG  G  GP G  G  GA G  G KG KG  G  G  G++G +   G  G++G +G  GK G +GS G+ GP G  GE G QGP GR GE+GP G  GP G                 G  G  G  G PG  GK G  GP+G  G QG AGE    G+RG PG AG  G++G  G  G  G  G  G  G  G  GP G  G +GSRG  G +G PG KG             G +G PG  GA+G  G  G  G  GP G++G P      G  GPIGP G+DGE
Sbjct:  346 GPPGDRGFDGLPGLPGNKGHR--QGSDGQPGPRGQPGEPGGRGVDGVQGSKGNLGSPGEPGAPGQQGNPGIMQGFPGPQGLVGLPGDKGPQG---------------------------------------KKGMRGLPGTDGPPQGHPGREGPPGEKGLPGLAGVQGPVGYPGARGVKGADGLRGIKGSKGEKGEDGFPGAKGEMGVKGDGGDTGPVGMRGEDGPEGPKGQSGPLGDPGSSGIAGEKGKLGVPGLPGYPGRQGPKGTDGFPGAIGVAGEKGRKGPPGQAGIAGQRGPN---GARGARGARGPTGKSGDKGSAGHDGPPGPSGERGPQGPQGRVGEIGPKGPSGPAGK---------------DGLPGHPGQRGEPGFQGKTGPPGPSGVVGPQGNAGETGPMGERGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTGVSGKNGPTGLKGFRGSRGAPGTMGPPGLKGSWGPVGSSGPGATGERGPPGLAGAIGQPGRTGTAGGPGPMGEKGEP-----QGEKGPIGPAGQDGE 824          

HSP 3 Score: 98.5969 bits (244), Expect = 4.881e-20
Identity = 208/572 (36.36%), Postives = 251/572 (43.88%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPG------KPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGK-----TGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEP----GSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGS------PGTIGAVGSKGE------------------------------PGPPGL---TGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            +GP G  G  GLPG      +PG  GK G  GP G +GP+G +G  GP G RG  G +GP GE G  G  GK G +G  G  G                                         G  G  G++G  G +G+ G +GP G KG  GP+G      TG +G  G  G  G  G+TG  G  GP G  GEP    G  G AG +GE+G  G  G+ GP GP G            E G KG+ G  G  G+ G  GE GP G TG  G EGQPG+ G  G++G   P G+QG+             G +G  G +G  G +GP+G++G   + GPPG KGE G  GL GP G  GP G  G  G                 G PG+ G++G  GG G +GE GE GD GP GI G+ G++G  G  G  G  G  G PG  G  G  G  G  G  GL G  G PG+ G   + G  GS      PG  G  G+ GE                              PG  GL   TGP G +GNPG  G  GL G  GP GE G
Sbjct:  651 KGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGVVGPQGNAGETGPMGERGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTG---------------------------------------VSGKNGPTGLKGFRGSRGAPGTMGPPGLKGSWGPVGSSGPGATGERGPPGLAGAIGQPGRTGTAGGPGPMGEKGEPQGEKGPIGPAGQDGEQGPVGLPGATGPPGPPG------------EDGDKGETGGPGQKGSKGDKGEPGPAGPTGTQGPEGQPGIPGVDGESG---PPGQQGM------------YGQKGDEGLRGFKGATGPRGLQG---MPGPPGEKGESGHSGLIGPPGQFGPRGPQGPSGGQQ--------------GPPGRPGVRGQPGGVGEKGEDGESGDPGPVGIPGSNGEKGDQGEKGDSGASGAGGIPGARGTSGEDGAKGNSGPIGLTGDPGPPGEAGTDGIDGAPGSKGDNGEPGKAGPPGASGEPGPPGRPGRRGHLGPPGKEGKPGMKGDKGAPGYEGLLGKTGPVGGQGNPGKAGPQGLPGIPGPAGEQG 1139          

HSP 4 Score: 92.8189 bits (229), Expect = 3.138e-18
Identity = 225/578 (38.93%), Postives = 279/578 (48.27%), Query Frame = 2
Query: 2486 RGPPGT------RGP---------------SGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGD---RGKFGPQGPT------GDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGK-----TGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEP----GSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT---------------GPQGKRGNPGPKGE------TGLMGPIGPVGEDG 4039
            +GPPG       RGP               SG  G  G DG  G  G +G  GP+G+ G  GP+G  G  G+ G  G  G +G+   +GK GP GP+      G+ GE G  G +G PG AGP G  G+ G+ G +G KG  G+ G  G  G  G++G  G +G+ G +GP G KG  GP+G      TG +G  G  G  G  G+TG  G  GP G  GEP    G  G AG +            G  GPVG+ G  G PGP GE G KG+ G  G  G+ G  GE GP G TG  G EGQPG+ G  G++   GP G+QG+             G +G  G +G  G +GP+G++   G+ GPPG KGE G  GL GP G  GP G  G  G                 G PG+ G++G  GG G +GE GE GD GP GI G+ G++G  G  G  G  G  G PG  G  G  G  G  G  GL    G+PG  GE    GI G+PG+ G  G  G+ GPPG +               GP GK G PG KG+       GL+G  GPVG  G
Sbjct:  588 KGPPGQAGIAGQRGPNGARGARGARGPTGKSGDKGSAGHDGPPGPSGERGPQGPQGRVGEIGPKGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGVVGPQGNAGETGPMGERGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTGVSGKNGPTGLKGFRGSRGAPGTMGPPGLKGSWGPVGSSGPGATGERGPPGLAGAIGQPGRTGTAGGPGPMGEKGEPQGEKGPIGPAGQD------------GEQGPVGLPGATGPPGPPGEDGDKGETGGPGQKGSKGDKGEPGPAGPTGTQGPEGQPGIPGVDGES---GPPGQQGM------------YGQKGDEGLRGFKGATGPRGLQ---GMPGPPGEKGESGHSGLIGPPGQFGPRGPQGPSGGQQ--------------GPPGRPGVRGQPGGVGEKGEDGESGDPGPVGIPGSNGEKGDQGEKGDSGASGAGGIPGARGTSGEDGAKGNSGPIGL---TGDPGPPGEAGTDGIDGAPGSKGDNGEPGKAGPPGASGEPGPPGRPGRRGHLGPPGKEGKPGMKGDKGAPGYEGLLGKTGPVGGQG 1118          

HSP 5 Score: 92.4337 bits (228), Expect = 3.778e-18
Identity = 200/552 (36.23%), Postives = 243/552 (44.02%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIR------GARGQSGPTGELGSK---GDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSV---GPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLP---GKAGLQGPAGGSGTQGEAGE-RGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGL---------------TGPQGKRGNPGP---KGETGLMGPIGPVGEDG 4039
            RG  G  G  G+ G  G  G++G  G KG++G +G  G+ GP G+R      G +GQSGP G+ GS    G++GK G  G  G  G +G  G+ G PG  G  G  G  G PG  G  G  G  G +G  G +G  G  G KGS    GP GP GE+G  GP G+ G  G +G  G  G +G  G  G RG  G  G+ G PG +GV                                  G +G+ G  GP G  G  G AGPPG+ G  GA G+ G KG  G TG+ G  G  GL                            G +G  G  G  GPPG KG  GP+G  GP  T                      G  G PGL    G+ G  G AGG G  GE GE +G++GP G AG  G++GP      VG  G  G PGP G  G  G  G  G +G  G  GEPG  G    +G +G PG  G  G  G PG  G+               TGP+G +G PGP   KGE+G  G IGP G+ G
Sbjct:  480 RGVKGADGLRGIKGSKGEKGEDGFPGAKGEMGVKGDGGDTGPVGMRGEDGPEGPKGQSGPLGDPGSSGIAGEKGKLGVPGLPGYPGRQGPKGTDGFPGAIGVAGEKGRKGPPGQAGIAGQRGPNGARGARGARGPTGKSGDKGSAGHDGPPGPSGERGPQGPQGRVGEIGPKGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGV---------------------------------VGPQGNAGETGPMGERGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTGVSGKNGPTGL---------------------------KGFRGSRGAPGTMGPPGLKGSWGPVGSSGPGAT----------------------GERGPPGLAGAIGQPGRTGTAGGPGPMGEKGEPQGEKGPIGPAGQDGEQGP------VGLPGATGPPGPPGEDGDKGETGGPGQKGSKGDKGEPGPAGPTGTQGPEGQPGIPGVDGESGPPGQQGMYGQKGDEGLRGFKGATGPRGLQGMPGPPGEKGESGHSGLIGPPGQFG 943          

HSP 6 Score: 87.8113 bits (216), Expect = 8.515e-17
Identity = 191/507 (37.67%), Postives = 221/507 (43.59%), Query Frame = 2
Query: 2567 KGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGI----KGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGE---AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSR---GLDGKVGEPGQKGERYLKGIKGSPGTIGA------VGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            KG  GP G +G PGP G  G  G  G +G  G  GDRG  G  G  G+KG R                                      QG  G+ G RG  G  G  G  G  G KG++G  G+ G  G +G  GI     G +G  GL G +GPQG  G  G PG  G                      QG PG+ GP GEKG+ G  G+ GP G PG        G  GA G  G  G KG KG+ G  G +GE G+                     +G  G++GP G+ GE G +GP   KG+ GP+G PG  G                   AG+ G +G PGLPG  G QGP G  G  G    AGE+G +GPPG AG  GQRGPNG  G  G  GP G+ G  G  G  G PGP G R   G  G+VGE G KG     G  G PG  G        G  G PGP G+ GPQG  G  GP GE G  G  GP GE G
Sbjct:  315 KGPPGPLGLNGRPGPLGNPGPTGLKGDSGLPGPPGDRGFDGLPGLPGNKGHR--------------------------------------QGSDGQPGPRGQPGEPGGRGVDGVQGSKGNLGSPGEPGAPGQQGNPGIMQGFPGPQGLVGLPGDKGPQGKKGMRGLPGTDG--------------------PPQGHPGREGPPGEKGLPGLAGVQGPVGYPGAR------GVKGADGLRGIKGSKGEKGEDGFPGAKGEMGV---------------------KGDGGDTGPVGMRGEDGPEGP---KGQSGPLGDPGSSGI------------------AGEKGKLGVPGLPGYPGRQGPKGTDGFPGAIGVAGEKGRKGPPGQAGIAGQRGPNGARGARGARGPTGKSGDKGSAGHDGPPGPSGERGPQGPQGRVGEIGPKGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGVVGPQGNAGETGPMGERGHPGSAGPPGEQG 715          

HSP 7 Score: 84.7297 bits (208), Expect = 8.898e-16
Identity = 194/549 (35.34%), Postives = 232/549 (42.26%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGKEGSIGPKGDLGPR----GQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPI------GPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGS------------PGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYL------------------KGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            PG +G  GLPG  G  GK+G  G  G  GP     G+ G PG +G+ G  G  GP G  G++G +G  G +G  G KGE+G+ G  G                                     KG  G+ G  G  GP+      GP+G KG  GPLG  G  G  GE G  G  G  G  G +GP+G  G PG+            PG AG+ G++G  G +G++G  GP G  G  G  G  G  G  G+ G  GP G  G IG  GP G  G  G  G PG +G  G  G  GP G  G+                   G QG+ G +GP G  G  G  GPPG +G  G  G  G  G  G +G  G  GP G  G  G  G  G+ G PG  G  GP G SG     G  G+RGPPG+AG +GQ G  G  G                 GP         +G  G +G  GQ GE+                    KG  G PG  G+ G KGEPGP G TG QG  G PG  G  G  GP G
Sbjct:  419 PGPQGLVGLPGDKGPQGKKGMRGLPGTDGPPQGHPGREGPPGEKGLPGLAGVQGPVGYPGARGVKGADGLRGIKGSKGEKGEDGFPGA------------------------------------KGEMGVKGDGGDTGPVGMRGEDGPEGPKGQSGPLGDPGSSGIAGEKGKLGVPGLPGYPGRQGPKGTDGFPGAIGVAGEKGRKGPPGQAGIAGQRGPNGARGARGARGPTGKSGDKGSAGHDGPPGPSGERGPQGPQGRVGEIGPKGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGV------------------VGPQGNAGETGPMGERGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTGVSGKNGPTGLKGFRGSRGAPGTMGPPGLKGSWGPVGSSGP----GATGERGPPGLAGAIGQPGRTGTAG-----------------GPGPMGEKGEPQGEKGPIGPAGQDGEQGPVGLPGATGPPGPPGEDGDKGETGGPGQKGSKGDKGEPGPAGPTGTQGPEGQPGIPGVDGESGPPG 892          

HSP 8 Score: 81.6481 bits (200), Expect = 7.655e-15
Identity = 200/518 (38.61%), Postives = 243/518 (46.91%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGD--KGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPD---------GEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGL 4006
            RG PG+ GP G  G PG  GKEG+   KGD G  G SG  GP G++G RG  G  G +G  G +G +GP G +G    GERG PG  G  G  G  G  G  G  G+KG               EKG  G  G+ G  GP+G           GE GD G  G  G KG++G+ G  G  G TG  G  G  G+ G  G  G  G  G  G++G +G +G  G  G +G+ G PGP GEKG  G  GLIGP   PG+ G  GP G +G  G +G PG  G +G  G +G +GE G  G  GP+G  G  G +G  G +G +G SG  G+ G  G  G  G KG  GPIGL G                               PG PG+AG  G  G  G++G+ GE G  GPPG +G                             GP G PG +G  G  GK G+PG KG++   G +G  G  G VG +G    PG  GPQG  G PGP GE GL
Sbjct:  702 RGHPGSAGPPGEQGLPGAAGKEGA---KGDPGSTGVSGKNGPTGLKGFRGSRGAPGTMGPPGLKGSWGPVGSSGPGATGERGPPGLAGAIGQPGRTGTAGGPGPMGEKGEPQG-----------EKGPIGPAGQDGEQGPVGLPGATGPPGPPGEDGDKGETGGPGQKGSKGDKGEPGPAGPTGTQGPEGQPGIPGVDGESGPPGQQGMYGQKGDEGLRGFKGATGPRGLQGMPGPPGEKGESGHSGLIGP---PGQFGPRGPQGPSG--GQQGPPGRPGVRGQPGGVGEKGEDGESGDPGPVGIPGSNGEKGDQGEKGDSGASGAGGIPGARGTSGEDGAKGNSGPIGLTG------------------------------DPGPPGEAGTDGIDGAPGSKGDNGEPGKAGPPGASGEP---------------------------GPPGRPGRRGHLGPPGKEGKPGMKGDKGAPGYEGLLGKTGPVGGQGN---PGKAGPQGLPGIPGPAGEQGL 1140          

HSP 9 Score: 80.1073 bits (196), Expect = 1.826e-14
Identity = 144/384 (37.50%), Postives = 178/384 (46.35%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDG------KEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKG---DRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIK---------------GSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP------GKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSG------------PQGVEGELGIQGPPGRKGELGPIGLPGPV 3514
            GP GT+GP G PG PG+DG      ++G  G KGD G RG  G  GP+G++G  G  G  GE G  G     G+FGP+GP G  G +                                             G  G+ G+ G VG  G DGE GD GP+G  G  G +G+ G K               G+ G+ G  G+ GP G++G+PG PG+AG +G  G  G +G  G  G  G  G  G+PGP G  G +G +G  G  G PG  G+ G P      GKTG  G +G PG  G +G  GI GP GEQGL+GP G  G  G  G  G PG +G  G  G            P G  GE G +G PG +G  GP G PGPV
Sbjct:  868 GPTGTQGPEGQPGIPGVDGESGPPGQQGMYGQKGDEGLRGFKGATGPRGLQGMPGPPGEKGESGHSGLIGPPGQFGPRGPQGPSGGQQG-----------------------------------------PPGRPGVRGQPGGVGEKGEDGESGDPGPVGIPGSNGEKGDQGEKGDSGASGAGGIPGARGTSGEDGAKGNSGPIGLTGDPGPPGEAGTDGIDGAPGSKGDNGEPGKAGPPGASGEPGPPGRPGRRGHLGPPGKEGKPGMKGDKGAPGYEGLLGKTGPVGGQGNPGKAGPQGLPGIPGPAGEQGLNGPPGQTGPPGPMGPSGLPGLKGDHGRKGDKGHGGLIGLIGPPGEFGEKGDRGMPGNQGVQGPKGDPGPV 1210          

HSP 10 Score: 73.1738 bits (178), Expect = 2.395e-12
Identity = 43/166 (25.90%), Postives = 76/166 (45.78%), Query Frame = 2
Query: 4241 DALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCI------SSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGD-SELSEENKFKY 4717
            + +F  +  +  +++ +R P G    S ARTC ++ +   +  D  Y+IDPN G  +D+ +  C F+ E +TC+       SVK     +N +KP      W +  R     S   D     V   Q+ FL+  ++ + Q   +LC  SV + D +  S +   ++
Sbjct: 1292 EEVFASLASMRTEVEGLRTPQG-TYHSPARTCKELWMWNPKLPD-VYWIDPNHGCHRDAFKAFCNFTAEGETCLQPHSKYQSVKLA--AWNKEKP----GTWFSKFRKGSQFSYI-DADGNDVHVVQLGFLKLLSATARQSFTYLCQNSVGWLDGATFSHKRALRF 1448          

HSP 11 Score: 67.0106 bits (162), Expect = 1.754e-10
Identity = 203/525 (38.67%), Postives = 237/525 (45.14%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGE------LGSKGD-RGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG---PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPG-------RIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGP---------RGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQK------------GERYLKGIKGSPGTIGAVGSKGEPGPP 3946
            RG PGT GP GL         +GS GP G  GP G +G  GP G+ GA GQ G TG       +G KG+ +G+ GP GP G  GE+G  G  G  GP GP G  G  G  G  G               +KG +G  G  G  GP G   P+G+ G  G  G++G  G +G  G KG EG  G  G+ GP+G+ G PG PG                                    EKG  G  GLIGP G  G         G+ GPPG+ G  G  G  G KG  G++G  GP         +G+QG  G  G  G+ G+ G RG  G  G+ GNSGP G+ G+    GPPG  G  G  G PG  G +G  GK G PG  G  G  G  G  G  G PGK G  G  G  G  G  G  G  GP G  GN G+ GP G  GI GP G +G  GP G  GP G  GP G  GL G  G  G K            GE   KG +G PG  G  G KG+PGP 
Sbjct:  750 RGAPGTMGPPGL---------KGSWGPVGSSGP-GATGERGPPGLAGAIGQPGRTGTAGGPGPMGEKGEPQGEKGPIGPAGQDGEQGPVGLPGATGPPGPPGEDGDKGETGGPG---------------QKGSKGDKGEPGPAGPTGTQGPEGQPGIPGVDGESGPPGQQGMYGQKGDEGLRGFKGATGPRGLQGMPGPPG------------------------------------EKGESGHSGLIGPPGQFGPRGPQGPSGGQQGPPGRPGVRGQPGGVGEKGEDGESGDPGPVGIPGSNGEKGDQGEKGDSGASGAGGIPGARGTSGEDGAKGNSGPIGLTGD---PGPPGEAGTDGIDGAPGSKGDNGEPGKAGPPGASGEPGPPGRPGRRGHLGPPGKEGKPGMKGDKGAPGYEGLLGKTGPVGGQGNPGKAGPQGLPGIPGPAGEQGLNGPPGQTGPPGPMGPSGLPGLKGDHGRKGDKGHGGLIGLIGPPGEFGEKGDRGMPGNQGVQGPKGDPGPV 1210          

HSP 12 Score: 56.6102 bits (135), Expect = 3.268e-7
Identity = 46/99 (46.46%), Postives = 53/99 (53.54%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            ++G  G  G SG  G  G +G+ G  GE G  G  G  G QG  G  G  G KGD G  G +G  G  G KG  GS G PG  G PG+KGS GPVG+ G
Sbjct:  674 FQGKTGPPGPSGVVGPQGNAGETGPMGERGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTGVSGKNGPTGLKGFRGSRGAPGTMGPPGLKGSWGPVGSSG 772          

HSP 13 Score: 56.225 bits (134), Expect = 3.771e-7
Identity = 46/101 (45.54%), Postives = 54/101 (53.47%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G +G +G  G  G  GPSG  G  G  G  G +GE G QG  GP G +GV G  G  GETGP G+   +G  GS GPPG  G PG  G  G  G+ G  G
Sbjct:  636 RGPQGPQGRVGEIGPKGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGVVGPQGNAGETGPMGE---RGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTG 733          

HSP 14 Score: 55.8398 bits (133), Expect = 5.284e-7
Identity = 49/129 (37.98%), Postives = 62/129 (48.06%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGD------------IGIQGETGPRGKDGNKGDEGSVGPPGY----------------AGNPGIKGSRGPVGNQGRDGE 2134
            G  G +GS G KG+ GP+G  GT+G  G  G+ G  G  GP G +G+ G KGD             G+QG  GP G+ G  G  G +GPPG                  G PG++G  G VG +G DGE
Sbjct:  850 GGPGQKGSKGDKGEPGPAGPTGTQGPEGQPGIPGVDGESGPPGQQGMYGQKGDEGLRGFKGATGPRGLQGMPGPPGEKGESGHSGLIGPPGQFGPRGPQGPSGGQQGPPGRPGVRGQPGGVGEKGEDGE 978          

HSP 15 Score: 55.0694 bits (131), Expect = 8.191e-7
Identity = 48/103 (46.60%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G+RG  G+KG+ G  G  G +GE+G+KG  G+ G  G RG +G  G KG  G  G+ G  G  G KG  G  G PGY G  G KG+ G  G  G  GEK
Sbjct:  483 KGADGLRGIKGSKGEKGEDGFPGAKGEMGVKGDGGDTGPVGMRGEDGPEGPKGQSGPLGDPGSSGIAGEKGKLGVPGLPGYPGRQGPKGTDGFPGAIGVAGEK 585          

HSP 16 Score: 54.6842 bits (130), Expect = 1.197e-6
Identity = 45/99 (45.45%), Postives = 53/99 (53.54%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G  G+ G  GP G  G RG  G +G  GE G +GP GP G  G+ G  G +GE G +GK G  G  G VGP G AG  G  G RG  G+ G  GE+
Sbjct:  616 GKSGDKGSAGHDGPPGPSGERGPQGPQGRVGEIGPKGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGVVGPQGNAGETGPMGERGHPGSAGPPGEQ 714          

HSP 17 Score: 53.1434 bits (126), Expect = 3.077e-6
Identity = 45/99 (45.45%), Postives = 56/99 (56.57%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +G  G KGD G +G VG RGE G +G KG+ G  G  G  GIAG KG +G+ G  G  G+ G KG +G    PG  G  G KG +GP G  G  G++
Sbjct:  505 GAKGEMGVKGDGGDTGPVGMRGEDGPEGPKGQSGPLGDPGSSGIAGEKGKLGVPGLPGYPGRQGPKGTDGF---PGAIGVAGEKGRKGPPGQAGIAGQR 600          

HSP 18 Score: 52.7582 bits (125), Expect = 4.573e-6
Identity = 42/101 (41.58%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G +G  G  G KG  GP+G+ G  G  G +G  G  G  GP GP G+ G +G+ G  G  G RG  G+ G  G  G PG AG  G KG  G  G  G++G
Sbjct:  639 QGPQGRVGEIGPKGPSGPAGKDGLPGHPGQRGEPGFQGKTGPPGPSGVVGPQGNAGETGPMGERGHPGSAGPPGEQGLPGAAGKEGAKGDPGSTGVSGKNG 739          

HSP 19 Score: 51.6026 bits (122), Expect = 9.847e-6
Identity = 47/122 (38.52%), Postives = 59/122 (48.36%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK---------------------DGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G +G SG  GD G SG  G +G++G+ G+ G  G QGP+G +G  G  G  G +G  GP G+                      G  GD+GS    G+ G PG  G RGP G QGR GE
Sbjct:  529 GPEGPKGQSGPLGDPGSSGIAGEKGKLGVPGLPGYPGRQGPKGTDGFPGAIGVAGEKGRKGPPGQAGIAGQRGPNGARGARGARGPTGKSGDKGSA---GHDGPPGPSGERGPQGPQGRVGE 647          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005318.1 (pep scaffold:Pmarinus_7.0:GL477895:43798:56979:-1 gene:ENSPMAG00000004829.1 transcript:ENSPMAT00000005318.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 134.806 bits (338), Expect = 1.180e-32
Identity = 219/749 (29.24%), Postives = 285/749 (38.05%), Query Frame = 2
Query: 2882 KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVG------------IKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVG------------SKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFD-KVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEG-------------AISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRT-PGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            KG  G  GP G  G+VG +G TG  G  GE G              G++GK G MG RG  G  GEPG PG  G+                            G +GE+G +G  G  G  GAPG  G  G  G  G SG  G+ GV G  G TG LG                                  SGP G +GE   +G PG  G  G  G PG  G  G SG DGA G  G +G  GD G  G+PG PG  G  G  G SG  G+ G RG+ GP G +G +G+ G  GP+G                            +G +G  GE G  GER +KG +G  G  G  G              G  GP G TGP G RG  G  G+ G +GP G  G  GE                                  G    + +   P+    RY    RA   +  +DA  D  +  +TN+++ I  P G   D  AR+C D+ L       G+Y+IDPNEGS  D+++V C   E  +TC+         Y   K +  +  W N    E                S   D  S  +   Q+NFLR   +++ Q I +LC  SV Y D+  S     + A  LM                   G ++ R      G+  F  ++  DGC   T     +  + +T   S LPI+D    D         G+ +  +CF
Sbjct:    1 KGPSGEGGPKGALGEVGGMGMTGFPGAPGESGTGGPPGGIGPQGASGAQGKDGSMGPRGDTGTPGEPGEPGPTGL---------------------------LGNQGERGERGPSGTRGTAGAPGSSGFLGSHGIQGLSGQRGERGVPGLNGPTGSLG---------------------------------KSGPTGEQGE---RGAPGNVGSPGLNGAPGESGREGSSGTDGASGRDGLAGVRGDRGEPGTPGRPGNPGNMGNPGLSGANGKDGHRGETGPQGDSGIVGRPGSAGPLG---------------------------PQGPNGAHGERGDSGERGIKGHRGWSGFQGLPGLPGPHGHQGGPGINGGSGPRGPTGPAGGRGKDGHNGQAGPLGPSGHRGRPGE--------AGSAGPAGSPGIPGPPGPPGPAANGYGQLPLIGMEKGPDPYPYRY----RADEALKEHDAEVDVTLKSLTNQLENIMSPEGTKTD-PARSCRDLMLCHSDWKSGEYWIDPNEGSVLDAMKVWCNM-ETGETCV---------YPKPKNVPSKNWWSNKGSREHKHVWFGEAMPGGFHFSYGDDTMSANIAEVQLNFLRLVAAEASQNITYLCRNSVAYADAAGS----LRKAVRLM-----------------GSGELELRA----DGKNRFTYSMLEDGCAKHTGEWGKTVLQYKTNKPSRLPIVDIAPLDIGGP-EQEFGVEIGPVCF 610          

HSP 2 Score: 74.7146 bits (182), Expect = 1.072e-13
Identity = 98/277 (35.38%), Postives = 124/277 (44.77%), Query Frame = 2
Query: 2534 GIDGKEGSIGPKGDLGPRGQSGNPGP------QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGP---LGKTGLKGTRGEVGIKGS------EGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILG 3319
            G  GK+GS+GP+GD G  G+ G PGP      QG RG RG SG  G  G+ G  G  G  G  G  G+R                                          GE+GV GL G  GS+G  GP GE+G+ G    +G  GL G  GE G +GS       G+ GL G RG +G  G PG PG+ G                 G  G+ G  G+ G RGE G +GD G++G  G+ G +G  GP G  G  G  G+ G+KG +G +G  G
Sbjct:   47 GAQGKDGSMGPRGDTGTPGEPGEPGPTGLLGNQGERGERGPSGTRGTAGAPGSSGFLGSHGIQGLSGQR------------------------------------------GERGVPGLNGPTGSLGKSGPTGEQGERGAPGNVGSPGLNGAPGESGREGSSGTDGASGRDGLAGVRGDRGEPGTPGRPGNPG---------------NMGNPGLSGANGKDGHRGETGPQGDSGIVGRPGSAGPLGPQGPNGAHGERGDSGERGIKGHRGWSGFQG 266          

HSP 3 Score: 61.2326 bits (147), Expect = 1.751e-9
Identity = 47/106 (44.34%), Postives = 60/106 (56.60%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQG---PRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNP---GIKGSRGPVGNQGRDG 2131
            G  G+ G+ G +G+ GPSG  GT G  G  G  G +GIQG    RG  G+ G+ G  G  G++GP G+ G +G  G+VG PG  G P   G +GS G  G  GRDG
Sbjct:   71 GPTGLLGNQGERGERGPSGTRGTAGAPGSSGFLGSHGIQGLSGQRGERGVPGLNGPTGSLGKSGPTGEQGERGAPGNVGSPGLNGAPGESGREGSSGTDGASGRDG 176          

HSP 4 Score: 60.8474 bits (146), Expect = 2.025e-9
Identity = 70/171 (40.94%), Postives = 86/171 (50.29%), Query Frame = 2
Query: 2852 EKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTG---------LKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            E G  GLLG +G  G  GP G +G  G  G +G         L G RGE G+ G  G TG +G  GP G  GE G+PG+ G        G  G+ G +G  G  G  G  G  G  GV+GD G  G  G PG  G  G PG +GA+G +G  G  G +GD+GI+G  G  G
Sbjct:   69 EPGPTGLLGNQGERGERGPSGTRGTAGAPGSSGFLGSHGIQGLSGQRGERGVPGLNGPTGSLGKSGPTGEQGERGAPGNVG------SPGLNGAPGESGREGSSGTDGASGRDGLAGVRGDRGEPGTPGRPGNPGNMGNPGLSGANGKDGHRGETGPQGDSGIVGRPGSAG 233          

HSP 5 Score: 57.3806 bits (137), Expect = 2.349e-8
Identity = 69/194 (35.57%), Postives = 82/194 (42.27%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAG 3067
            RGP GTRG +G PG  G     GS G +G  G RG+ G PG  G  G+ G+SGPTGE G +G  G  G  G  G  GE                                           G +G  G  G  G  G  G  G++G+ G  G+ G  G  G  G+ G+ GK G  G  GPQG SG  G PG AG
Sbjct:   85 RGPSGTRGTAGAPGSSGF---LGSHGIQGLSGQRGERGVPGLNGPTGSLGKSGPTGEQGERGAPGNVGSPGLNGAPGE------------------------------------------SGREGSSGTDGASGRDGLAGVRGDRGEPGTPGRPGNPGNMGNPGLSGANGKDGHRGETGPQGDSGIVGRPGSAG 233          

HSP 6 Score: 50.447 bits (119), Expect = 3.309e-6
Identity = 42/100 (42.00%), Postives = 51/100 (51.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            GA+G  GS G +GD G  G+ G  G  G+ G +GE G +GP G  G AG  G  G  G  G +G  G +G+ G    PG  G  G  G  GP G QG  G
Sbjct:   47 GAQGKDGSMGPRGDTGTPGEPGEPGPTGLLGNQGERGERGPSGTRGTAGAPGSSGFLGSHGIQGLSGQRGERGV---PGLNGPTGSLGKSGPTGEQGERG 143          

HSP 7 Score: 50.0618 bits (118), Expect = 4.130e-6
Identity = 43/104 (41.35%), Postives = 53/104 (50.96%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHG---PSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G  G  GSSG  G HG    SGQ G RG  G+ G  G  G  GP G +G  G  G++G  G  G  G+ G +G  G+ G  G  G  G++G RG  G  GR G
Sbjct:   91 RGTAGAPGSSGFLGSHGIQGLSGQRGERGVPGLNGPTGSLGKSGPTGEQGERGAPGNVGSPGLNGAPGESGREGSSGTDGASGRDGLAGVRGDRGEPGTPGRPG 194          

HSP 8 Score: 49.6766 bits (117), Expect = 5.565e-6
Identity = 42/102 (41.18%), Postives = 51/102 (50.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G RG RG  G  G  G  G+ G  GE G +G  G  G  G  G  G +G +G  G  G +G  G  G +GD G  G PG  GNPG  G+ G  G  G+DG +
Sbjct:  113 GQRGERGVPGLNGPTGSLGKSGPTGEQGERGAPGNVGSPGLNGAPGESGREGSSGTDGASGRDGLAGVRGDRGEPGTPGRPGNPGNMGNPGLSGANGKDGHR 214          

HSP 9 Score: 49.6766 bits (117), Expect = 6.060e-6
Identity = 43/102 (42.16%), Postives = 50/102 (49.02%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G  GSSG  G  G  G  G RG+ G  G  G  G  G  G  G++G  G  G +GETGP+G  G  G  GS GP G  G  G  G RG  G +G  G +
Sbjct:  158 GESGREGSSGTDGASGRDGLAGVRGDRGEPGTPGRPGNPGNMGNPGLSGANGKDGHRGETGPQGDSGIVGRPGSAGPLGPQGPNGAHGERGDSGERGIKGHR 259          

HSP 10 Score: 49.2914 bits (116), Expect = 7.628e-6
Identity = 58/174 (33.33%), Postives = 71/174 (40.80%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGL 3007
            RG PG  G  GL G PG  G+EGS G  G       SG  G  G+RG RG+ G  G  G+ G+ G  G  G  G  G R                                       G  G +G  G++GR GS GP+GP G  G  G  G +G +G +G  G  G +G  GL
Sbjct:  142 RGAPGNVGSPGLNGAPGESGREGSSGTDG------ASGRDGLAGVRGDRGEPGTPGRPGNPGNMGNPGLSGANGKDGHR---------------------------------------GETGPQGDSGIVGRPGSAGPLGPQGPNGAHGERGDSGERGIKGHRGWSGFQGLPGL 270          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009617.1 (pep scaffold:Pmarinus_7.0:GL476473:139194:170406:1 gene:ENSPMAG00000008685.1 transcript:ENSPMAT00000009617.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 96.6709 bits (239), Expect = 2.590e-20
Identity = 199/515 (38.64%), Postives = 233/515 (45.24%), Query Frame = 2
Query: 2516 GLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIG-------LIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G+PG+ G  G  G+ G +GD G +G  G PG  G RG  G  GP G  G+ GD+G  GP GPTG                                                  G RG  G +G  GP GP G  G  G  G+ G KG +GE G KG        G+ GPQG SG PG  G  GV+G                               KG +G  G       L GP      +   G PG  G +G  G+ G KG +GD+G +G  GE GL GP+G  G KG  G  G PG  G  G    QG+ G+ GI G PG++GE G  GLPGP G  G  G  GA G  G  G  G  G  G PG  G+ G  G  G  G  G AG +G+RG  G AG  G  G  G  G VGP G  G  G  GP GP+G PGP G+RGL G  G  G KGE    G +G  G  G  G +G PGPPG +G QG  G+ GP G  GL GP GP G+DG
Sbjct:  149 GMPGERGATGISGAKGDRGDSGEKGPEGAPGKDGGRGVTGPIGPPGPAGAHGDKGDAGPAGPTG------------------------------------------------PSGARGAPGERGETGPPGPAGFAGPPGSDGQPGAKGEQGETGQKGD------AGAPGPQGPSGAPGPQGPNGVSG------------------------------PKGARGAQGPPPGARALCGPAVDVAPVPFQGNPGSPGPAGPNGKDGPKGIRGDSGPVGRAGEPGLQGPIGAPGEKGDAGEDGPPGPDGPPGA---QGLSGQRGIVGLPGQRGERGFPGLPGPAGEPGKQGSTGASGERGPPGPVGPPGLSGPPGEQGREGNAGSDGAPGRDGAAGAKGERGEAGAAGQPGAPGSPGAPGPVGPTGKNGARGDQGPQGPMGPPGPSGARGLPGPQGPRGDKGETGEAGERGIKGHRGFTGLQGLPGPPGSSGDQGLAGSVGPSGPRGLSGPTGPPGKDG 576          

HSP 2 Score: 87.4261 bits (215), Expect = 1.957e-17
Identity = 70/269 (26.02%), Postives = 108/269 (40.15%), Query Frame = 2
Query: 4196 RYKRSTRAQSFVDINDALFD-KVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHT--------QMNFLRNQNSKSYQ-----EIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDY 4960
            RY RS +A   +  +DA  D  +  ++ +I+ +R P G  K+ A RTC D+KL   +   G Y+IDPN+G   D+++V C   E  +TC+ +  Q     N+    + ++  +  +    A+S    LH  Y D          QM FLR  + ++Y       I + C  S+ Y D E       K A +L   +   I      R   N        +  E+GR      +EY                 T   S LP +D    D 
Sbjct:  637 RYYRSDQALPELRQHDAEVDASIKSLSGQIEGLRSPEGTRKNPA-RTCRDLKLCHPEWESGNYWIDPNQGCTLDALKVFCNM-ETGETCVYANPQTISRKNW---WQSKSADRKHVWFGEAMSGG--LHFNYGDDNLPANTAAIQMTFLRLLSMEAYDLPAAANITYHCKNSIAYMDEET---GNLKKALLLQGSNDMEIRAEGNSRFTYNVLEDSCTKHTGEWGR----TVVEY----------------RTQKTSRLPFVDVAPMDV 875          

HSP 3 Score: 74.3294 bits (181), Expect = 1.778e-13
Identity = 170/426 (39.91%), Postives = 195/426 (45.77%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXX-XXXXXXXXXXXXXXXXXEKGVRG------LLGRKGSVGPIGPDGEKGDV---------GPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQ 3718
            GP G  GPSG  G PG  G+ G  GP G  GP G  G PG +G +G  GQ G  G  G +G  G  GPQGP G  G +G  G+QGPP  A       V  +P   +G  G  G  GP G  G KG+RG        G  G  GPIG  GEKGD          GP G  GL G RG VG+ G  G+ G  G  GP   +GEPG  G  G +            G  GP G  G PG  GP GE+G +G+ G     GAPGR G AG  G+ G +GA GQPG  G+ G  G +GP G+ G  G  GP G  G  G  G  G  G  G  G +G  GE G +G  G +G  G  GLPGP                                 PG +G QG AG  G  G  G  G  GPPG  GN GQ
Sbjct:  206 GPAGPTGPSGARGAPGERGETGPPGPAGFAGPPGSDGQPGAKGEQGETGQKGDAGAPGPQGPSGAPGPQGPNGVSGPKGARGAQGPPPGARALCGPAVDVAPVPFQGNPGSPGPAGPNGKDGPKGIRGDSGPVGRAGEPGLQGPIGAPGEKGDAGEDGPPGPDGPPGAQGLSGQRGIVGLPGQRGERGFPGLPGP---AGEPGKQGSTGAS------------GERGPPGPVGPPGLSGPPGEQGREGNAGS---DGAPGRDGAAGAKGERGEAGAAGQPGAPGSPGAPGPVGPTGKNGARGDQGPQGPMGPPGPSGARGLPGPQGPRGDKGETGEAGERGIKGHRGFTGLQGLPGP---------------------------------PGSSGDQGLAGSVGPSGPRGLSGPTGPPGKDGNNGQ 580          

HSP 4 Score: 61.6178 bits (148), Expect = 1.567e-9
Identity = 167/472 (35.38%), Postives = 196/472 (41.53%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGK---------------------TGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPT------GAPGRIGEAGPPGKTGAS---GAEGQPGVK------GTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQG----PPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA---------------------------GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---------PGPKGETGLMGPIGPVGEDGE 4042
            +G RG  G +G+ GP G  G +G  G  G                       G+ G RG  GI G++G  G  G +GP+G    PG  G  GV                      G  G PGP G  G KGD G  GPT      GAPG  GE GPPG  G +   G++GQPG K      G KGD G  GP+G  G  GP                         GP GV G  G +G    PPG +   GP     PV   G  G  G  GP G  G  G  G  G  G  G+ GLQGP G  G +G+A                           G+RG+RG PG+ G  G+ G  G  G  G  GP G  GP G  GP G  G +G+ G DG  G  G  G +  +G  G+ G  GA GS G PGP G TG  G RG+         PGP G  GL GP GP G+ GE
Sbjct:   97 RGERGFPGERGAPGPQGLQGARGLPGTPGTDGPKGATGPGGPPGAQGPPGLQGMPGERGATGISGAKGDRGDSGEKGPEGA---PGKDGGRGVT---------------------GPIGPPGPAGAHGDKGDAGPAGPTGPSGARGAPGERGETGPPGPAGFAGPPGSDGQPGAKGEQGETGQKGDAGAPGPQGPSGAPGP------------------------QGPNGVSGPKGARGAQGPPPGARALCGPAVDVAPVPFQGNPGSPGPAGPNGKDGPKGIRGDSGPVGRAGEPGLQGPIGAPGEKGDAGEDGPPGPDGPPGAQGLSGQRGIVGLPGQRGERGFPGLPGPAGEPGKQGSTGASGERGPPGPVGPPGLSGPPGEQGREGNAGSDGAPGRDGAAGAKGERGEAGAAGQPGAPGSPGAPGPVGPTGKNGARGDQGPQGPMGPPGPSGARGLPGPQGPRGDKGE 520          

HSP 5 Score: 58.151 bits (139), Expect = 1.681e-8
Identity = 160/442 (36.20%), Postives = 190/442 (42.99%), Query Frame = 2
Query: 2924 DVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSR---GPQGVSGE---PGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR---------------------IGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLP----GKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGI---KGSPGTIGA------------------------------VGSKGEPGPPGLTGPQGKR------GNPGPKGETGLMGPIGPVGEDG 4039
            + G  G  G+ G  G+VG+ G+ G+ G  G R   GPQG+ G    PG+PG  G  G  G  G  G+QGP G  G+ G  G  G  G KG +GD G  GP GAPG+                      G+AGP G TG SGA G PG +G  G  G  G  G  G D                        G  G +G +GE G +G  G  G  GP G PGP G +G SG  GA G  G    A           P    G  G  GPAG +G  G  G RGD GP G AG  G +   GPIG  G  G  G+ GP GP GP G  G  G RG+   VG PGQ+GER   G+    G PG  G+                               GS G PG  G  G +G+R      G PG  G  G  GP+GP G++G
Sbjct:   75 EAGKPGAQGVPGEAGKVGVSGARGERGFPGERGAPGPQGLQGARGLPGTPGTDGPKGATGPGGPPGAQGPPGLQGMPGERGATGISGAKGDRGDSGEKGPEGAPGKDGGRGVTGPIGPPGPAGAHGDKGDAGPAGPTGPSGARGAPGERGETGPPGPAGFAGPPGSD------------------------GQPGAKGEQGETGQKGDAGAPGPQGPSGAPGPQGPNGVSGPKGARGAQGPPPGARALCGPAVDVAPVPFQGNPGSPGPAGPNGKDGPKGIRGDSGPVGRAGEPGLQ---GPIGAPGEKGDAGEDGPPGPDGPPGAQGLSGQRGI---VGLPGQRGERGFPGLPGPAGEPGKQGSTGASGERGPPGPVGPPGLSGPPGEQGREGNAGSDGAPGRDGAAGAKGERGEAGAAGQPGAPGSPGAPGPVGPTGKNG 486          

HSP 6 Score: 53.9138 bits (128), Expect = 3.488e-7
Identity = 52/105 (49.52%), Postives = 57/105 (54.29%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPP---GYAGNPGIKGSRGPVGNQGRDGEK 2137
            G RG  G SGAKGD G SG+ G  G  G  G +G  G  GP GP G  G KGD G  G TGP G  G  G+ G  GPP   G+AG PG  G  G  G QG  G+K
Sbjct:  152 GERGATGISGAKGDRGDSGEKGPEGAPGKDGGRGVTGPIGPPGPAGAHGDKGDAGPAGPTGPSGARGAPGERGETGPPGPAGFAGPPGSDGQPGAKGEQGETGQK 256          

HSP 7 Score: 50.8322 bits (120), Expect = 2.640e-6
Identity = 39/91 (42.86%), Postives = 48/91 (52.75%), Query Frame = 2
Query: 1865 KGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
             GD G +G  G  G  G +G  GE G  GP GP G AG  G  G  G  G +G+ G KGD G+ GP G +G PG +G  G  G +G  G +
Sbjct:  199 HGDKGDAGPAGPTGPSGARGAPGERGETGPPGPAGFAGPPGSDGQPGAKGEQGETGQKGDAGAPGPQGPSGAPGPQGPNGVSGPKGARGAQ 289          

HSP 8 Score: 50.0618 bits (118), Expect = 5.423e-6
Identity = 47/131 (35.88%), Postives = 60/131 (45.80%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQ----------------------------GETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            + G  G  G  GAKG+ G +GQ   +G+ G  G +G  G  GP+GP G++G KG  G Q                            G  GP GKDG KG  G  GP G AG PG++   GP+G  G  G+
Sbjct:  234 FAGPPGSDGQPGAKGEQGETGQ---KGDAGAPGPQGPSGAPGPQGPNGVSGPKGARGAQGPPPGARALCGPAVDVAPVPFQGNPGSPGPAGPNGKDGPKGIRGDSGPVGRAGEPGLQ---GPIGAPGEKGD 358          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002207.1 (pep scaffold:Pmarinus_7.0:GL476843:46518:72113:-1 gene:ENSPMAG00000001999.1 transcript:ENSPMAT00000002207.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.8189 bits (229), Expect = 5.783e-20
Identity = 75/268 (27.99%), Postives = 117/268 (43.66%), Query Frame = 2
Query: 4217 AQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTD-GQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGG--LYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            A  + D  + +F  +  +  +I+ I+ PTG  + S  RTC D++L +    D G+YYIDPN+G   DS  V C F+   +TCI   K+  G  L  + K   G   W +  +     S   DL    +   QM FLR  +S + Q   + C  SV + D     E +  YA  L               +G NE  M++       G P  ++   YDGC  R     +  ++ T  +  +PI+D  ++D+ +      G  V  +CF
Sbjct:  146 AVDYPDGMEEIFGSISSLKREIELIKHPTG-AQSSPGRTCKDLQLSQHDLPDAGEYYIDPNQGCSADSFRVYCNFTAGGETCIYPDKKSEGARLSAWSKETPG--TWYSEYKRGKQFSYV-DLDGQGIGTVQMTFLRLLSSAARQNFTYNCHHSVGWLD-----EAEGSYARAL-------------RFMGANEQEMERG------GSP--HIRALYDGCSMRRGYEKTVLEIHTHKVQQMPIVDVLVSDFGDPH-QKFGFEVGPVCF 382          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001576.1 (pep scaffold:Pmarinus_7.0:GL476350:400893:424819:1 gene:ENSPMAG00000001417.1 transcript:ENSPMAT00000001576.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 94.7449 bits (234), Expect = 9.039e-20
Identity = 79/261 (30.27%), Postives = 115/261 (44.06%), Query Frame = 2
Query: 4196 RYKRSTRAQSFVDINDALFDKVDK-ITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCI----SSVKQQGGLYNYKKPMK----GETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLE--TTDLSMLPIIDF 4945
            R+ R  +A + +  +DA  D   K +TN+I  IR P G  K+  ARTC D+KL   +   G Y+IDPN+G   D+I+V C   E  +TC+     +V ++    N  K  K    GET    S    G  S  P+  ++     QM FLR  ++++ Q I + C  S+ Y D +       K A +L                   +G  D  +  +   R  +NV    DGC   T G   +  LE  T   S LPI+D 
Sbjct:  494 RFYRGDQASADLRQHDAEVDATLKSLTNQIDSIRSPEGSRKN-PARTCRDLKLCHPEWKSGDYFIDPNQGCTLDAIKVFCNM-ESGETCVYASPQNVPRKSWWTNKSKDRKHIWFGETMNSGSQFNYGDDSLPPNTAAI-----QMTFLRLLSTEASQNITYHCKNSIAYMDEQA---GNLKKALLL-------------------QGSNDIEIRAEGNSRFTYNVL--EDGCTKHT-GDWGQTVLEYRTQKTSRLPIVDI 722          

HSP 2 Score: 58.5362 bits (140), Expect = 1.397e-8
Identity = 87/206 (42.23%), Postives = 102/206 (49.51%), Query Frame = 2
Query: 2486 RGPPGTRG------PSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGP---------LGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPG 3058
            RG PG RG      P+G  G PG DG+ G+ G +G+ G +G +G PGPQG  G+ G  GP G  G KG RG  GP G TG  G             AG  GP G  G+PG  G  G  G  GP+G  G+ G  G  G  G  G  GP GEKG+ G           G  GL G RG VG+ G  G+ G  G  GP G  G+ GSPG
Sbjct:   75 RGSPGERGETGPPGPAGFAGPPGSDGQPGAKGEQGESGQKGDAGAPGPQGPAGSPGPQGPNGVPGPKGARGAQGPSGATGFPG------------AAGRVGPPGPNGNPGPPGPPGPGGKDGPKGIRGDSGPAGRSGEPGLQGAAGPPGEKGEPGEDGPSGPEGPPGPMGLAGQRGIVGLPGQRGERGFSGLPGPSGEPGKQGSPG 268          

HSP 3 Score: 49.6766 bits (117), Expect = 6.948e-6
Identity = 47/142 (33.10%), Postives = 63/142 (44.37%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIG------------------------IQGETGPRGKDGNKGDEGSVGPPGYAGNPG---------------IKGSRGPVGNQGRDGEK 2137
            +G  G +G +GA G  GP+G  G +G  G+ G KG  G QGP G  G  G  G +G                        I+G++GP G+ G  G +G+ GPPG  G PG               + G RG VG  G+ GE+
Sbjct:  108 QGESGQKGDAGAPGPQGPAGSPGPQGPNGVPGPKGARGAQGPSGATGFPGAAGRVGPPGPNGNPGPPGPPGPGGKDGPKGIRGDSGPAGRSGEPGLQGAAGPPGEKGEPGEDGPSGPEGPPGPMGLAGQRGIVGLPGQRGER 249          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003197.1 (pep scaffold:Pmarinus_7.0:GL479090:2162:37629:-1 gene:ENSPMAG00000002618.1 transcript:ENSPMAT00000003197.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 95.1301 bits (235), Expect = 9.630e-20
Identity = 74/282 (26.24%), Postives = 117/282 (41.49%), Query Frame = 2
Query: 4208 STRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGA-------ISRAPDLHSMYVDHT-----QMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLE--TTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            +  AQ+  + N  L   +  +  +++ +  P G  K+ A RTC ++KL       G Y+IDPNEGS  D+I+V C   E  ++C+          N   P     NW   L+  G+       ++ +PD    Y + +     Q+ FLR  ++++ Q+I + C  SV Y D  +      + A +L                G NE  M+ R      G      ++  DGC   T G   K  +E  T   S LPI+D    D         G++V  +CF
Sbjct:  945 ADEAQNQGERNLELEASISSLNVRVENMVSPDGSQKNPA-RTCRNLKLCYPDLPSGLYWIDPNEGSKSDAIQVWCNM-ETGESCM----------NADNPSIARKNWW--LKPSGSKKHVWFGVTMSPDAQFTYGEDSHSTEIQLTFLRLFSTEASQKITYHCKNSVAYQDGAV---GNLQQALLLQ---------------GSNE--MEIRAE----GNSKLAYSVLEDGCTMHT-GQWGKTVIEYRTPKTSRLPIVDIAPKDVGGP-DQEFGVDVGPVCF 1186          

HSP 2 Score: 63.929 bits (154), Expect = 2.946e-10
Identity = 65/179 (36.31%), Postives = 81/179 (45.25%), Query Frame = 2
Query: 2546 KEGSIGPKGDLGPRGQSGNPG-----PQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAG 3067
            KEG+ GP+G+ GP G +G PG     P G +GA G  GP G  GS G  G  G +G  G  G+RG+ G                                         G  GL+G  G  GP+GP GE+G  GP G  G  G+ G+ G  G+ G  GL G  GPQG  G+ G PG+AG
Sbjct:  654 KEGTKGPRGETGPPGSTGAPGAGVTGPSGEKGAPGDDGPVGAEGSPGPSGLAGSRGVVGLPGQRGERGF---------------------------------------SGPAGLVGEAGKQGPVGPSGERGSAGPAGAMGSAGSPGDSGRDGAAGADGLPGRDGPQGPKGDRGPPGNAG 793          

HSP 3 Score: 60.4622 bits (145), Expect = 3.776e-9
Identity = 121/301 (40.20%), Postives = 139/301 (46.18%), Query Frame = 2
Query: 3170 KGVKGDIGLIGPTGAPGR-----IGEAGPPGKTGASGAEGQPG---VKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP---GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            KG +G+ G  G TGAPG       GE G PG  G  GAEG PG   + G++G  G+ G RGE+G                            SGP G+ GE G QGP G  GE G  G  G +G++G  G  G  G  G              GLPG+ G QGP G  G  G AG  G  G  G +G  G  G  G  G VGP GP G  GP GP GP+G  G  G RG +G  GE G KG R   G+ G PG  G  G  G P   GP G  GP G  G+ G  G  G  GP+GP G  G
Sbjct:  658 KGPRGETGPPGSTGAPGAGVTGPSGEKGAPGDDGPVGAEGSPGPSGLAGSRGVVGLPGQRGERGF---------------------------SGPAGLVGEAGKQGPVGPSGERGSAGPAGAMGSAGSPGDSGRDGAAGAD------------GLPGRDGPQGPKGDRGPPGNAGPAGAPGASGPSGQAGSPGRTGERGDVGPAGPSGPAGPPGPRGPVGPLGGLGERGDNGAAGERGHKGHRGFAGLPGPPGPPGLSGESGTPGPAGPAGARGPVGGAGSHGKDGANGAPGPLGPAGPRG 919          

HSP 4 Score: 56.9954 bits (136), Expect = 4.336e-8
Identity = 46/103 (44.66%), Postives = 59/103 (57.28%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G  G  GA+G  GPSG  G+RG VG+ G +GE G  GP      AG+ G+ G QG  GP G+ G+ G  G++G  G  G+ G  G+ G  G  GRDG +
Sbjct:  684 KGAPGDDGPVGAEGSPGPSGLAGSRGVVGLPGQRGERGFSGP------AGLVGEAGKQGPVGPSGERGSAGPAGAMGSAGSPGDSGRDGAAGADGLPGRDGPQ 780          

HSP 5 Score: 50.8322 bits (120), Expect = 2.881e-6
Identity = 105/304 (34.54%), Postives = 128/304 (42.11%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPG--IDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRG------KFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSP---GDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP------------TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRG 3328
            +GP G  GP G  G PG  + G  G  G  GD GP G  G+PGP G+ G+RG  G  G+ G +G  G      + G QGP G  GER                                          G  G  G +G  GS G  G DG  G  G  G+ G +G +G+ G  G+ G  G  G+ GP G +G PG     GD G  G  G  G  G +GP GP+G  G  G  G  GE+G KG  G  G             +G PG  G AG  G  G +G+ G+ G  G  G  G  GPRG
Sbjct:  658 KGPRGETGPPGSTGAPGAGVTGPSGEKGAPGDDGPVGAEGSPGPSGLAGSRGVVGLPGQRGERGFSGPAGLVGEAGKQGPVGPSGER------------------------------------------GSAGPAGAMGSAGSPGDSGRDGAAGADGLPGRDGPQGPKGDRGPPGNAGPAGAPGASGPSGQAGSPGRTGERGDVGPAGPSGPAGPPGPRGPVGPLGGLGERGDNGAAGERGHKGHRGFAGLPGPPGPPGLSGESGTPGPAGPAGARGPVGGAGSHGKDGANGAPGPLGPAGPRG 919          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Nematostella
Match: EDO44729 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV66])

HSP 1 Score: 136.732 bits (343), Expect = 3.392e-32
Identity = 256/881 (29.06%), Postives = 342/881 (38.82%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGV---SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMG---------------------------------QRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGIST---LGLNVLSMCF 5011
            RG  G  GP G  G PG  G EG  GP G  G  G  GN G +G  G +G  G  G  G KG  G+ G +GP GD GE                                     G +GP GE G  G+ G  G  GPIG  G +G  GP G  GL G RG  G +GS G  G+ G +GP GV    G+ G+PGD G                              P  E GV G  GL GP G  G  GE G  GK G  G  G  G +G++GD G +G R + G                             G +G  G +G QG  GR G  G  G+ GP G  G  G  G  GPPG  G  G        G  GKAG  G  G  G +G  G+ G  GP G  G++G                                  RGP GP+GI                        KG RG  G +G+ G KG   L G +G  G  G +G KG  G  G  G  G+RG  GP GE G++G  G +G  G  G+ G +G  G PG  G +G  G  G  G  G+PG    + +               +  + + +ND     + ++ +K K    P G ++ + A TC ++K       DG+Y+IDPN+G   D+I+V C F    ++C+    +      +         W    +LE    R   + +   +  Q+NFLR   +K  Q+I   C   V++        +   Y      H F       G +   N       V +D           +YD   +R     ++   ETT L  LPI+DF    Y ++ ++T    G+ +  +CF
Sbjct:  406 RGAAGPSGPRGALGHPGPQGLEGDRGPIGKPGVSGTKGNIGAKGRIGLQGDRGEQGPFGPKGAGGEMGSKGPPGDFGE------------------------------------VGVRGPNGELGSEGVPGEVGKPGPIGYQGREGFKGPNGLPGLPGKRGLRGSEGSTGVYGMKGMKGPIGVPGFRGQHGTPGDIG-----------------------------PPGSESGVPGKTGLRGPMGHKGPDGEKGYVGKRGEKGFMGLQGDQGSRGDRGPIGERAKDG---------------------------EKGLKGETGPIGGQGLEGRTGVQGDDGIRGPQGKQGGVGPTGGHGPPGPMGDDG------DAGSKGKAGKIGTKGEPGRKGSPGDIGYVGPKGFLGDVGPLGEKGGQGAYGPLGEPGGPGPKGGGGPKGKLGDRGPQGPVGI------------------------KGIRGASGPIGDSGIKGGYGLMGPRGPDGNPGLLGRKGRQGEKGTAGRLGERGKKGPFGENGVLGKRGTLGPPGSPGSIGDMGAKGHPGSLGSKGRRGKLGPPGEPGKPGEILNLHL--------------LKQNTHLIVNDKKSLYLSEMDDKSKT---PLG-SRANPALTCHELK-HVNGIKDGEYWIDPNQGCSSDAIKVYCKFKTGGESCVRPALKMIPKETWNNAT--TAGWYQFSKLE----RGYKI-TYLTNKVQLNFLRLLTTKVTQKIIVKCKSVVVW-----FHRHSKSYDYSWKFHGF------NGHQFTYNSSRSSLNVLRD---------ACKYDDGRWR----RTELLFETTKLRTLPIVDFT---YYHRSLTTPEEFGVKIEPVCF 1111          

HSP 2 Score: 96.2857 bits (238), Expect = 8.777e-20
Identity = 140/402 (34.83%), Postives = 177/402 (44.03%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGI------AGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +GV+GLLG +G  GP G  G  G  G +G  GL G +GE   KG EG+ G+ G RG +G SG PG                                G  GP G  G KG IG+IG +G+PG+ G  GP G++G +GA G    KG KG  G +GP G QG                             G  G++G+ G  G  G KG  G  G+PGP GT GP G  G  G  G            +PG+PG  G  GP+G  G QG  G +GD G  G+      AGN G  GP G +G +G                        ++G+ G VG PG  G R L G +G  G +   G  G PGPPG  G  GK G  G +G  G+ G  G +G+ G+
Sbjct:   19 QGVQGLLGDRGPRGPPGEQGFSGVKGAVGSHGLIGRKGE---KGKEGRDGVKGERGSKGKSGLPGD------------------------------KGPVGPPGHPGHKGAIGIIGKSGSPGKDGSEGPTGESGPTGALG---AKGQKGGVGSIGPSGLQGF---------------------------QGRMGLKGDAGYDGVDGFKGHAGNTGIPGPQGTMGPIGAKGILGDVG------------APGMPGGVGSIGPSGLQGFQGRMGLKGDAGYDGVDGFKGHAGNTGIPGPQGTMGPIG------------------------AKGILGDVGAPGMPGSRGLNGTRGPEGDL---GHSGLPGPPGFQGDPGKPGIRGHEGLQGVSGKAGSIGDPGD 318          

HSP 3 Score: 76.6406 bits (187), Expect = 7.300e-14
Identity = 130/426 (30.52%), Postives = 175/426 (41.08%), Query Frame = 2
Query: 2852 EKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGS---EGKTGLMGSRGPQGVSGEPGS---PGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGE---AGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIK---------------------GSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
             +G  G  G +G++G  GP G +GD GP+GK G+ GT+G +G KG    +G  G  G  GP+G  GE GS   PGD G                                 E GV+G  G +G  G PG +G+ GP G  G  G +G  G+ G  G  G+ G  G  G+                            G +G++G +G+ G  G+ G  G IG PG                                G+PGK GL+GP G  G  GE    G+RG++G  G+ G+ G RG  GPIG         +    G  G  G  GP G +GL+G+ G  G  G R  +G +                     GS G  G +G+KGEP      G +G  G+ G  G  G +G +GP+GE G
Sbjct:  405 SRGAAGPSGPRGALGHPGPQGLEGDRGPIGKPGVSGTKGNIGAKGRIGLQGDRGEQGPFGPKGAGGEMGSKGPPGDFG---------------------------------EVGVRGPNGELGSEGVPGEVGKPGPIGYQGREGFKGPNGLPGLPGKRGLRGSEGSTGV---------------------------YGMKGMKGPIGVPGFRGQHGTPGDIGPPG-----------------------------SESGVPGKTGLRGPMGHKGPDGEKGYVGKRGEKGFMGLQGDQGSRGDRGPIG---------ERAKDGEKGLKGETGPIGGQGLEGRTGVQGDDGIRGPQGKQGGVGPTGGHGPPGPMGDDGDAGSKGKAGKIGTKGEP------GRKGSPGDIGYVGPKGFLGDVGPLGEKG 726          

HSP 4 Score: 72.7886 bits (177), Expect = 1.141e-12
Identity = 132/423 (31.21%), Postives = 171/423 (40.43%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEV------GIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIV 3748
            G  G  G PG  G  G +G  G  GD GPRG    PG QG  G +G  G  G +G KG++GK G  G  G++G +                        G  G  G  G  GP G  G KG  G++G+ GS G  G +G  G+ GP G  G KG +G V      G++G +G+ GL G  G  GV G  G  G+                                     G+ G  G +GP GA G +G+ G PG  G  G+ G  G++G +G  G+ G  G  G+D                     G  G++G  G+ G  G  GP G KG LG +G PG  G                           S GL G  G +G  G SG  G  G +GD G PGI G+ G +G +G  G +
Sbjct:    2 GLMGSRGTPGDQGGRGPQGVQGLLGDRGPRGP---PGEQGFSGVKGAVGSHGLIGRKGEKGKEGRDGVKGERGSK------------------------GKSGLPGDKGPVGPPGHPGHKGAIGIIGKSGSPGKDGSEGPTGESGPTGALGAKGQKGGVGSIGPSGLQGFQGRMGLKGDAGYDGVDGFKGHAGNT------------------------------------GIPGPQGTMGPIGAKGILGDVGAPGMPGGVGSIGPSGLQGFQGRMGLKGDAGYDGVD---------------------GFKGHAGNTGIPGPQGTMGPIGAKGILGDVGAPGMPG---------------------------SRGLNGTRGPEGDLGHSGLPGPPGFQGDPGKPGIRGHEGLQGVSGKAGSI 313          

HSP 5 Score: 71.633 bits (174), Expect = 2.869e-12
Identity = 106/285 (37.19%), Postives = 139/285 (48.77%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGL 3340
            +G  G++G SGLPG  G  G  G  G KG +G  G+SG+PG  G  G  G+SGPTG LG+KG +G  G  GP+G +G +G  G +G  G  G +G  G AG+ G  G                +G  G +G KG +G +G  G  G VG +G +GL+G +G +G+KG  G  G+ G +G  G +G PG  G  G                              P G KG+ GD+G  G  G+ G  G  GP G  G SG  G PG +G  G  GI G  G QG+
Sbjct:   67 KGERGSKGKSGLPGDKGPVGPPGHPGHKGAIGIIGKSGSPGKDGSEGPTGESGPTGALGAKGQKGGVGSIGPSGLQGFQGRMGLKGDAGYDGVDGFKGHAGNTGIPG---------------PQGTMGPIGAKGILGDVGAPGMPGGVGSIGPSGLQGFQGRMGLKGDAGYDGVDGFKGHAGNTGIPGPQGTMG------------------------------PIGAKGILGDVGAPGMPGSRGLNGTRGPEGDLGHSGLPGPPGFQGDPGKPGIRGHEGLQGV 306          

HSP 6 Score: 67.781 bits (164), Expect = 3.596e-11
Identity = 122/369 (33.06%), Postives = 154/369 (41.73%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGE---------------------LGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPD---------GEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPV 3514
            G RGP G PG+ G  G +G++G  G +G +G+ G  G  G++G RG  G +G                      +G  G  GK G +GPTG+ G                                               G  G  G+KG VG IGP          G KGD G  G  G KG  G  GI G +G  G +G++G  G  G PG PG  G  G  G QG QG  G  G  G  G+ G  G  G  G+ G  G +GP GA G +G+ G PG  G+ G  G  G +G  G +G+ GP G QG                   PG+ G  G+ G QGV G+ G  G PG  G+ G  GLPG +
Sbjct:   26 GDRGPRGPPGEQGFSGVKGAVGSHGLIGRKGEKGKEGRDGVKGERGSKGKSGLPGDKGPVGPPGHPGHKGAIGIIGKSGSPGKDGSEGPTGESG---------------------------------------------PTGALGAKGQKGGVGSIGPSGLQGFQGRMGLKGDAGYDGVDGFKGHAGNTGIPGPQGTMGPIGAKGILGDVGAPGMPGGVGSIGPSGLQGFQGRMGLKGDAGYDGVDGFKGHAGNTGIPGPQGTMGPIGAKGILGDVGAPGMPGSRGLNGTRGPEGDLGHSGLPGPPGFQG------------------DPGKPGIRGHEGLQGVSGKAGSIGDPGDPGDEGNEGLPGDI 331          

HSP 7 Score: 56.9954 bits (136), Expect = 7.238e-8
Identity = 44/106 (41.51%), Postives = 63/106 (59.43%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGI-AGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAG---NPGIKGSRGPVGNQGRDGEK 2137
            G RG+RGS G+ G +G     G +G +G+ G +G++G  G  GP G  +GV G  G++G  G +G DG KG  G  G  G+ G   + G +G RGP+G + +DGEK
Sbjct:  527 GKRGLRGSEGSTGVYG---MKGMKGPIGVPGFRGQHGTPGDIGPPGSESGVPGKTGLRGPMGHKGPDGEKGYVGKRGEKGFMGLQGDQGSRGDRGPIGERAKDGEK 629          

HSP 8 Score: 55.8398 bits (133), Expect = 1.849e-7
Identity = 51/128 (39.84%), Postives = 65/128 (50.78%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGP---------RGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY----------------AGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G  GS G  GD G  G  G  GE+G +GV GE G  GP         +GP G+ G+ G  G++G  G  G  G KG +G +G PG+                +G PG  G RGP+G++G DGEK
Sbjct:  466 KGAGGEMGSKGPPGDFGEVGVRGPNGELGSEGVPGEVGKPGPIGYQGREGFKGPNGLPGLPGKRGLRGSEGSTGVYGMKGMKGPIGVPGFRGQHGTPGDIGPPGSESGVPGKTGLRGPMGHKGPDGEK 593          

HSP 9 Score: 54.6842 bits (130), Expect = 3.574e-7
Identity = 47/103 (45.63%), Postives = 54/103 (52.43%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +GA G  G  G +GD GP G+ G  G  G  G KG  G+QG RG +G  G KG  G  G  GP G  G  G  G  G  G  G PG  G  GP+G QGR+G K
Sbjct:  415 RGALGHPGPQGLEGDRGPIGKPGVSGTKGNIGAKGRIGLQGDRGEQGPFGPKGAGGEMGSKGPPGDFGEVGVRGPNGELGSEGVPGEVGKPGPIGYQGREGFK 517          

HSP 10 Score: 54.299 bits (129), Expect = 4.969e-7
Identity = 45/107 (42.06%), Postives = 57/107 (53.27%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSV------GPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G +G +G  G KGD G  G  G +G  G  G+ G    QG  GP G  G+ GD+G  G  G RG +G +G EG +      GPPG+ G+PG  G RG  G QG  G+
Sbjct:  206 GLQGFQGRMGLKGDAGYDGVDGFKGHAGNTGIPGP---QGTMGPIGAKGILGDVGAPGMPGSRGLNGTRGPEGDLGHSGLPGPPGFQGDPGKPGIRGHEGLQGVSGK 309          

HSP 11 Score: 53.9138 bits (128), Expect = 6.967e-7
Identity = 43/101 (42.57%), Postives = 56/101 (55.45%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KGA GI G SG+ G  G  G  G  G  G  G KG+ G  G  GP G+ G +G +G++G+ G  G DG KG  G+ G PG  G  G  G++G +G+ G  G
Sbjct:   94 KGAIGIIGKSGSPGKDGSEGPTGESGPTGALGAKGQKGGVGSIGPSGLQGFQGRMGLKGDAGYDGVDGFKGHAGNTGIPGPQGTMGPIGAKGILGDVGAPG 194          

HSP 12 Score: 52.7582 bits (125), Expect = 1.313e-6
Identity = 47/111 (42.34%), Postives = 58/111 (52.25%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSG-------QVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGP---RGKDGNKGDEGSVGP---PGYAGNPGIKGSRGPVGNQGR 2125
            G  G RG  G  GD GP G       + G RG +G KG  GE G  G RG +G  G++GD G +G+ GP   R KDG KG +G  GP    G  G  G++G  G  G QG+
Sbjct:  551 GVPGFRGQHGTPGDIGPPGSESGVPGKTGLRGPMGHKGPDGEKGYVGKRGEKGFMGLQGDQGSRGDRGPIGERAKDGEKGLKGETGPIGGQGLEGRTGVQGDDGIRGPQGK 661          

HSP 13 Score: 52.7582 bits (125), Expect = 1.635e-6
Identity = 43/97 (44.33%), Postives = 55/97 (56.70%), Query Frame = 2
Query: 1853 SSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQ---GETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            S GA G  GP G +G  G  G++G +G  G  G  G +G  G KG IG+Q   GE GP G  G  G+ GS GPPG  G  G++G  G +G++G  GE
Sbjct:  405 SRGAAGPSGPRGALGHPGPQGLEGDRGPIGKPGVSGTKGNIGAKGRIGLQGDRGEQGPFGPKGAGGEMGSKGPPGDFGEVGVRGPNGELGSEGVPGE 501          

HSP 14 Score: 52.373 bits (124), Expect = 1.841e-6
Identity = 44/102 (43.14%), Postives = 56/102 (54.90%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA+G  G  G +G+ GP G  G  GE+G KG  G++G  G RGP G  G +G  G  G+ GP G  G +G +G  G PG  G  G++GS G  G  G  G K
Sbjct:  446 GAKGRIGLQGDRGEQGPFGPKGAGGEMGSKGPPGDFGEVGVRGPNGELGSEGVPGEVGKPGPIGYQGREGFKGPNGLPGLPGKRGLRGSEGSTGVYGMKGMK 547          

HSP 15 Score: 51.6026 bits (122), Expect = 3.410e-6
Identity = 44/109 (40.37%), Postives = 59/109 (54.13%), Query Frame = 2
Query: 1871 DHGPSGQVGTRG------EVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETG------------PRGKDGNKGDEGSVGPP---GYAGNPGIKGSRGPVGNQGRDGE 2134
            D GP G VG +G       +G  G+KG YG+ GPRGP+G  G+ G  G QGE G            P G++G  G  G++GPP   G  G+ G KG  G +G++GR G+
Sbjct:  754 DRGPQGPVGIKGIRGASGPIGDSGIKGGYGLMGPRGPDGNPGLLGRKGRQGEKGTAGRLGERGKKGPFGENGVLGKRGTLGPPGSPGSIGDMGAKGHPGSLGSKGRRGK 862          

HSP 16 Score: 50.8322 bits (120), Expect = 5.029e-6
Identity = 47/126 (37.30%), Postives = 61/126 (48.41%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGP------RGKDGNKGDEGSVGPPGYA------------------GNPGIKGSRGPVGNQGRDGEK 2137
            G  G+RG  G KG  G  G VG RGE G  G++G+ G +G RGP G     G+ G++GETGP       G+ G +GD+G  GP G                    G+ G KG  G +G +G  G K
Sbjct:  576 GKTGLRGPMGHKGPDGEKGYVGKRGEKGFMGLQGDQGSRGDRGPIGERAKDGEKGLKGETGPIGGQGLEGRTGVQGDDGIRGPQGKQGGVGPTGGHGPPGPMGDDGDAGSKGKAGKIGTKGEPGRK 701          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Nematostella
Match: EDO42953 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S046])

HSP 1 Score: 136.732 bits (343), Expect = 4.209e-32
Identity = 288/900 (32.00%), Postives = 395/900 (43.89%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGK---PGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVR--GLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP---------------GKTGASGAEGQPGVKGTKGDTGILGPRG------EQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQK------------GERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPK------------------GETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGR-YKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCFS 5014
            RGP G +GP G PG    PG+ GK G  GP+G+   RG  G PG  G RG +G++GPTG  G            PTG  G+RG  G+ G P                                   + +R   ++G  G+ GP       G+ G  G+ G+ G  G  G  G++G  G  G  GP G+ G+ G+PG  G+NG  G QG  G +G  G  G  G  G      + G +G  GL G  GAPGR GE G                 G  G  G+ G+PG+ GT G  G LG +G       QG+ G  GP G  G  G  G PG  G +G++G  GV+G  G  G PGR GE G  G PG  G  G  G DG PG  G  G+ G  G  G  G PG  G +GP G  G +GE G  G  G  G  G +G RGP G  G  G  G  G+PG  G  G  G PG KG RG  G+ G+ G++            GE  ++G KG+ G  G  G +G  GP G  GPQG +GN GP+                  G+ G  GP G  G DG  G  G +GP GP G PG  G PG  G  G  G     G + +    +G   R Y  S  A+   + + +    ++K+   +  IR PTG  K++ A+TC D+++       G Y+IDPNEGS KD+I V C FS +  +CIS  K +  L N +  ++   N ++    E     +   +S   + +Q+ FLR  + ++YQ + + C     + + + S + +     +      K    +                        I N     D   ++T        ++T     LPI+D  + D+  K    + + V S+CF+
Sbjct:  596 RGPVGPQGPQGAPGTQGPPGLPGKTGESGPRGE---RGTDGKPGAMGERGPQGETGPTGLTG------------PTGKPGDRGSNGADGTPVSK-------------------------------HEFIRYFAIIGEPGAQGP------SGNPGTPGRQGMPGASGRRGAPGAKGSRGERGRVGPPGIDGKDGAPGSDGLNGPPGPQGPAGEKGEAGQPGTNGFNG------KNGDQGPRGLPGTPGAPGRNGEQGASGPTGPVGPRGPGGIKGAKGVMGSSGRPGITGTLGQRGRLGSKGGEGTPGSQGMPGMSGPPGRPGPPGPPGPPGPSGPSGSNGRNGVKGSTGEGGRPGRDGEPGEPGTPGRDGEPGIPGPDGRPGERGPGGEIGRKGEKGPSGPPGITGNRGPPGSPGIKGERGYPGKDGRAGEPGAVGLRGPTGQPGENGLPGEPGKPGINGTNGRGGIPGLKGERGSPGEPGKAGKQGPPGPSGPLGANGEPGVQGAKGARGDDGKDGKQGPAGPQGPNGPQGDKGNRGPRGEKGDKGFTGSDGSPGAPGQQGEQGPPGVAGTDGPPGPRGNVGPMGPMGDPGLPGPPGPPGPPGYTGLSPVGGYLSVGTVEKGPGYRLYAGSKDAKKPDNPSVSFMTALEKMEIDVNTIRKPTGM-KNNPAKTCKDLRMDHPDYVSGYYWIDPNEGSKKDAIRVYCDFSNDF-SCISPTKAK--LLNLR--VQANVNKKDKWLSEAFEEESQIDYS--AEQSQIAFLRVFSKRAYQNVTYNCQNYKAWTNRKHSIKLRGDNDLIFHAKSPKKFQPTV-----------------------ILNECTTKDDQWHKT-----ILAIDTKQKHRLPIVDMALFDF-GKPHQKVTVEVGSVCFA 1400          

HSP 2 Score: 93.5893 bits (231), Expect = 6.013e-19
Identity = 204/566 (36.04%), Postives = 239/566 (42.23%), Query Frame = 2
Query: 2495 PGTRGPSGLPGKPGIDGK------------------EGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGP------------------LGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXX---XXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR---------IGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGE--RYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            PG  G  G PG PG +GK                  +G  G  G+ G RG  GNPG +G RGA      TG  GS G++G  GP GP G KG+ G  G  G PG  G  G +G  G  G  G +G  G PG  G  G  G+ G  G  G  G IG  GEKG  GP                  +G+ G  G  G+ G +G+ G  G  G+ GP G  GE GSPG AG  G KG++G  G  G  G     G  G PG PG  G+ G+ GD G  G  GAPG+          G  GP G  GA GA G+PG  G  G  G  G +GE GL                           +G +G+ G  G  G  G  GE GP+G  GP G                       G  G PGLPGK G  GP G  GT G+ G  G+RGP G  G  G  GP G  G                            RG +G  G P  K E  RY   I G PG  G  G+ G PG  G+ G  G+RG PG KG  G  G +GP G DG+
Sbjct:  251 PGFDGAPGAPGSPGAEGKVGLTGQTGPRGPTGPPGLQGVRGAPGNEGARGLPGNPGLKGKRGA------TGSRGSPGNQGPQGPSGPQGSKGQAGIDGRPGEPGLPGDKGAMGGPGPAGQPGIRGPSGAPGITGNPGATGINGDRGAPGRPGKIGSKGEKGSEGPNGSPGPRGAPGPPGKRGKIGRVGPMGPIGDPGSRGATGPRGFPGAAGPNGRKGEQGSPGAAGFPGSKGDKGSSGKNGEPGRVGLRGPPGQPGSPGREGKTGLPGDDGQDGKPGAPGKPDRLSLQGMTGMPGPSGVAGAKGANGEPGKDGDTGPQGPAGAKGESGL---------------------------AGSRGMPGVAGKNGNAGEPGERGPVGPQGPQGA---------------------PGTQGPPGLPGKTGESGPRGERGTDGKPGAMGERGPQGETGPTGLTGPTGKPG---------------------------DRGSNGADGTPVSKHEFIRYFA-IIGEPGAQGPSGNPGTPGRQGMPGASGRRGAPGAKGSRGERGRVGPPGIDGK 734          

HSP 3 Score: 86.6557 bits (213), Expect = 8.342e-17
Identity = 210/548 (38.32%), Postives = 269/548 (49.09%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGEL------------GSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGR---KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGS---------------RGPQGVSGEPGSPGDAGVNXXXXXX------XXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGEL---GPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIA---GNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGL 4006
            GP G+ GP G  G+ G+ G+ GS G +G  GP    G+PG  G  GA G+ G  GE             G KG+ G  G +G  G  G  G  G+ G PG  G  G +G+ G  G +G  G  G  G +G  G +G RGL G    KG  G  G  G  G+ GP G +G +G++G+ GI G  G+ GL G                RGP G  G  G+PG  G+NG++G        G +G +G  GP G  G  G PGP G++G  G +G +GP G PG  G  GP G  GA+G  G+ G +G+ G  G  G +G++G  G  G  G  GL+G  G+PG  G  G +G  G +G+ G  G PG+   L   G  G+PGP G +G  G +                     G PGK G  GP G +G +GE+G  G RG PG+A   GN G+ G  GP+G               P GP G PG +G  GL GK GE G +GER   G  G PG +G  G +GE GP GLTGP GK G+ G  G  G 
Sbjct:  165 GPRGSDGPRGQRGEVGLPGESGSAGRRGASGP---PGSPGIDGFSGAPGEQGEKGEAGPSGPPGFPGLKGQKGEEGNLGERGMVGRPGRPGFDGAPGAPGSPGAEGKVGLTGQTGPRGPTGPPGLQGVRGAPGNEGARGLPGNPGLKGKRGATGSRGSPGNQGPQGPSGPQGSKGQAGIDGRPGEPGLPGDKGAMGGPGPAGQPGIRGPSGAPGITGNPGATGINGDRGAPGRPGKIGSKGEKGSEGPNGSPGPRGAPGPPGKRGKIGRVGPMGPIGDPGSRGATGPRGFPGAAGPNGRKGEQGSPGAAGFPGSKGDKGSSGKNGEPGRVGLRGPPGQPGSPGREGKTGLPGDDGQDGKPGAPGKPDRLSLQGMTGMPGPSGVAGAKGAN---------------------GEPGKDGDTGPQGPAGAKGESGLAGSRGMPGVAGKNGNAGEPGERGPVG---------------PQGPQGAPGTQGPPGLPGKTGESGPRGER---GTDGKPGAMGERGPQGETGPTGLTGPTGKPGDRGSNGADGT 670          

HSP 4 Score: 76.6406 bits (187), Expect = 8.470e-14
Identity = 201/547 (36.75%), Postives = 258/547 (47.17%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGEL---GSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGR---KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGS---------------RGPQGVSGEPGSPGDAGVNXXXXXXX------XXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGEL---GPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GP G RG  GLPG+ G  G+ G+ GP G  G  G SG PG QG +G  G SGP G     G KG+ G  G +G  G  G  G  G+ G PG  G  G +G+ G  G +G  G  G  G +G  G +G RGL G    KG  G  G  G  G+ GP G +G +G++G+ GI G  G+ GL G                RGP G  G  G+PG  G+NG++G  G       +G +G  GP G  G  G PGP G++G  G +G +GP G PG  G  GP G  GA+G  G+ G +G+ G  G  G +G++G  G  G  G  GL+G  G+PG  G  G +G  G +G+ G  G PG+   L   G  G+PGP G +G  G +                     G PGK G  GP G +G +GE+G  G RG PG+AG                                           +G  GEPG++G    +G +G+PGT      +G PG PG TG  G RG  G  G+ G MG  GP GE G
Sbjct:  171 GPRGQRGEVGLPGESGSAGRRGASGPPGSPGIDGFSGAPGEQGEKGEAGPSGPPGFPGLKGQKGEEGNLGERGMVGRPGRPGFDGAPGAPGSPGAEGKVGLTGQTGPRGPTGPPGLQGVRGAPGNEGARGLPGNPGLKGKRGATGSRGSPGNQGPQGPSGPQGSKGQAGIDGRPGEPGLPGDKGAMGGPGPAGQPGIRGPSGAPGITGNPGATGINGDRGAPGRPGKIGSKGEKGSEGPNGSPGPRGAPGPPGKRGKIGRVGPMGPIGDPGSRGATGPRGFPGAAGPNGRKGEQGSPGAAGFPGSKGDKGSSGKNGEPGRVGLRGPPGQPGSPGREGKTGLPGDDGQDGKPGAPGKPDRLSLQGMTGMPGPSGVAGAKGAN---------------------GEPGKDGDTGPQGPAGAKGESGLAGSRGMPGVAGK------------------------------------------NGNAGEPGERGPVGPQGPQGAPGT------QGPPGLPGKTGESGPRGERGTDGKPGAMGERGPQGETG 648          

HSP 5 Score: 75.0998 bits (183), Expect = 2.453e-13
Identity = 191/555 (34.41%), Postives = 225/555 (40.54%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDG----------------EKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSR------GPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPP---------------GKTGASGAEGQPGVKGTKGDTGILGPR------GEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGE 4033
            G+RG  G+ GK G  G+ G  GP G  GP+G  G  GP G+ G  G+SGP GE G+ G  G  G +GP G+ G                                               G+ G  G+ G  G  G DG                E G  GP G  G  G +G  G  G  G  G  GSR      GP G+ G+ G+PG  G+N      G  G QGP G  G  G PG  G  G+ G +G  GL G  GAPGR GE G                 G  G  G+ G+PG+ GT G  G LG +      G QG+ G  GP G  G  G  G PG  G +G++G  GV+G  G  G PGR GE G  G PG  G                            PG+PG  G  G  G  G  G  GE+G  GPPGI GN   RGP G  GI                        KG RG  GK G  G+ G   L+G  G PG  G     GEPG PG+ G  G+ G PG KGE G  G  G  G+
Sbjct:  576 GSRGMPGVAGKNGNAGEPGERGPVGPQGPQGAPGTQGPPGLPGKTGESGPRGERGTDGKPGAMGERGPQGETG---------------------------------------------PTGLTGPTGKPGDRGSNGADGTPVSKHEFIRYFAIIGEPGAQGPSGNPGTPGRQGMPGASGRRGAPGAKGSRGERGRVGPPGIDGKDGAPGSDGLN------GPPGPQGPAGEKGEAGQPGTNGFNGKNGDQGPRGLPGTPGAPGRNGEQGASGPTGPVGPRGPGGIKGAKGVMGSSGRPGITGTLGQRGRLGSKGGEGTPGSQGMPGMSGPPGRPGPPGPPGPPGPSGPSGSNGRNGVKGSTGEGGRPGRDGEPGEPGTPGRDG---------------------------EPGIPGPDGRPGERGPGGEIGRKGEKGPSGPPGITGN---RGPPGSPGI------------------------KGERGYPGKDGRAGEPGAVGLRGPTGQPGENGL---PGEPGKPGINGTNGRGGIPGLKGERGSPGEPGKAGK 1022          

HSP 6 Score: 73.1738 bits (178), Expect = 1.013e-12
Identity = 188/541 (34.75%), Postives = 226/541 (41.77%), Query Frame = 2
Query: 2507 GPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQG------VSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSG---------TQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGP---------------KGETGLMGPIGPVGEDG 4039
            G +GLPG  G DGK G+ G    L  +G +G PGP G+ GA+G +G   E G  GD    GPQGP G KGE                               G+ G+ G  G  G+ G  G  G +G VGP GP G  G  GP G  G  G  G  G +G++GK G MG RGPQG      ++G  G PGD G NG  G    +                         + G+ G  GP+G PG  G  G PG   ASG  G PG KG++G+ G +GP G  G DG  G  G  G  G +G  G          +G  G+ G  G  G+ G+ GP GLPG                              +PG PG+ G QG +G +G          +G  G  G  G PGI G +GQR      G +G  G  G PG  G  G  G PG  G  G  G  G  G  G     G+KGS G  G  G  GEPG PG  G  G+ G PGP               KGE G  GP G  G  G
Sbjct:  507 GKTGLPGDDGQDGKPGAPGKPDRLSLQGMTGMPGPSGVAGAKGANG---EPGKDGD---TGPQGPAGAKGE------------------------------SGLAGSRGMPGVAGKNGNAGEPGERGPVGPQGPQGAPGTQGPPGLPGKTGESGPRGERGTDGKPGAMGERGPQGETGPTGLTGPTGKPGDRGSNGADGTPVSKHEFIRYFA-----------------IIGEPGAQGPSGNPGTPGRQGMPG---ASGRRGAPGAKGSRGERGRVGPPGIDGKDGAPGSDGLNGPPGPQGPAGE---------KGEAGQPGTNGFNGKNGDQGPRGLPG------------------------------TPGAPGRNGEQGASGPTGPVGPRGPGGIKGAKGVMGSSGRPGITGTLGQR------GRLGSKGGEGTPGSQGMPGMSGPPGRPGPPGPPGPPGPSGPSGSNGRNGVKGSTGEGGRPGRDGEPGEPGTPGRDGEPGIPGPDGRPGERGPGGEIGRKGEKGPSGPPGITGNRG 946          

HSP 7 Score: 72.4034 bits (176), Expect = 1.828e-12
Identity = 143/412 (34.71%), Postives = 177/412 (42.96%), Query Frame = 2
Query: 2903 GPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERY------------------LKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGP---------------KGETGLMGPIGPVGEDG 4039
            GPDG  GD+G  G +G  G  G +G  G  G  G+ G  GPQG  G  G PG +                        GI G        G+KG  G  GP G+PG  G  G  G  G  G  G PG  G+ G  G  GP G  G+DG  G  G +G KG  G  G  G  G  G +G EG LG +G  GR G  G  G PG  G+ G  GK G  G  G  G  G  G  G  G PG  G +G  G  G +G+ G  G RG PG   N G +GP+GP G                         KG  G+DG+ GEPG  G++                     GI G+PG  G  G +G PG PG  G +G++G+ GP               +G+ G +GP+GP+G+ G
Sbjct:   76 GPDGPPGDIGDQGPSGEAGKPGPLGYNGQPGNQGMRGDPGPQGDQGPQGPPGGS-------------------TQAAGGIKGPDPNDLAAGIKGSNGAPGPRGSPGLPGPRGSDGPRGQRGEVGLPGESGSAGRRGASGPPGSPGIDGFSGAPGEQGEKGEAGPSGPPGFPGLKGQKGEEGNLGERGMVGRPGRPGFDGAPGAPGSPGAEGKVGLTGQTGPRGPTGPPGLQGVRGAPGNEGARGLPGNPGLKGKRGATGSRGSPG---NQGPQGPSGPQG------------------------SKGQAGIDGRPGEPGLPGDKGAMGGPGPAGQPGIRGPSGAPGITGNPGATGINGDRGAPGRPGKIGSKGEKGSEGPNGSPGPRGAPGPPGKRGKIGRVGPMGPIGDPG 441          

HSP 8 Score: 66.6254 bits (161), Expect = 8.358e-11
Identity = 200/552 (36.23%), Postives = 245/552 (44.38%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGI------------RGARGQSGPTGELGSKGDRGKFGPQ------GPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG-PD-----GEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPG-PVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDR---GPPGIAGNMGQRGPNG---PIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER------------YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG 4012
            RGP G  G +G PG  GI+G  G+ G  G +G +G+ G+ GP G             RG  G+ GP G +G  G RG  GP+      GP G KGE+G PG+ G PG  G  G  G  G PG  G +G  G PG  G  G+ G+ G  G+ G  G  G PD     G  G  GP G  G KG  GE G  G  G  G  G++G  G++G  G PG AG N                           G  GE G +G +G  GP GAPG  G  G PGKTG SG  G+   +GT G  G +G R                                 GPQG  G  G+ GP G+ G+ G  G  G PV       K          G+ G  GP G+PG PG+ G+ G +G  G  G  G RG+R   GPPGI G  G  G +G   P G  GP G +G+ G  G  G  G  G +G RGL G  G PG+ GE+               GIKG+ G +G+ G       PG+TG  G+RG  G KG  G  G
Sbjct:  368 RGPSGAPGITGNPGATGINGDRGAPGRPGKIGSKGEKGSEGPNGSPGPRGAPGPPGKRGKIGRVGPMGPIGDPGSRGATGPRGFPGAAGPNGRKGEQGSPGAAGFPGSKGDKGSSGKNGEPGRVGLRGPPGQPGSPGREGKTGLPGDDGQDGKPGAPGKPDRLSLQGMTGMPGPSGVAGAKGANGEPGKDGDTGPQGPAGAKGESGLAGSRGMPGVAGKN---------------------------GNAGEPGERGPVGPQGPQGAPGTQGPPGLPGKTGESGPRGE---RGTDGKPGAMGER---------------------------------GPQGETGPTGLTGPTGKPGDRGSNGADGTPV------SKHEFIRYFAIIGEPGAQGPSGNPGTPGRQGMPGASGRRGAPGAKGSRGERGRVGPPGIDGKDGAPGSDGLNGPPGPQGPAGEKGEAGQPGTNGFNGKNGDQGPRGLPGTPGAPGRNGEQGASGPTGPVGPRGPGGIKGAKGVMGSSGR------PGITGTLGQRGRLGSKGGEGTPG 844          

HSP 9 Score: 63.5438 bits (153), Expect = 8.723e-10
Identity = 197/589 (33.45%), Postives = 237/589 (40.24%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRG------LLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP---QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGT------------KGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGE------------------LGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQ------------GEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLK------------------------GIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G RGP G PG              GD GP G++G PGP G  G  G  G  G+ G +GD+   GPQGP G   +                                              G++G        G KGS G  GP G  G  GP G  G +G RGEVG+ G  G  G  G+ GP    G+ G  G+PG+ G                 G  G  G PG PG +G+KG +G++G  G  G PGR G  G PG  G+ GAEG+ G+ G             +G  G  G  G +GL G  G  G +G  G RG PG QG  G SGPQG +G+ GI G PG  G                    GP G PG  G                 G  G +G  G+PG PGK G +G  G  G              G+ G+ G  GP G  G+ G RG  GP G  G  GP G+ G  G  G  G PG KG +G  GK GEPG+ G R                           G  G P  +   G  G PGP G+ G +G  G PG  G+TG  GP G  GE G
Sbjct:   73 GQRGPDGPPGD------------IGDQGPSGEAGKPGPLGYNGQPGNQGMRGDPGPQGDQ---GPQGPPGGSTQAAG-------------------------------------------GIKGPDPNDLAAGIKGSNGAPGPRGSPGLPGPRGSDGPRGQRGEVGLPGESGSAGRRGASGPPGSPGIDGFSGAPGEQGEK---------------GEAGPSGPPGFPGLKGQKGEEGNLGERGMVGRPGRPGFDGAPGAPGSPGAEGKVGLTGQTGPRGPTGPPGLQGVRGAPGNEGARGLPGNPGLKGKRGATGSRGSPGNQGPQGPSGPQGSKGQAGIDGRPGEPGLPGDKGAMGGPGPAGQPGIRGPSGAPGITGN---------------PGATGINGDRGAPGRPGKIGSKGEKGSEGPNGSPGPRGAPGPPGKRGKIGRVGPMGPIGDPGSRGATGPRGFPGAAGPNGRKGEQGSPGAAGFPGSKGDKGSSGKNGEPGRVGLRGPPGQPGSPGREGKTGLPGDDGQDGKPGAPGKPDRLSLQGMTGMPGPSGVAGAKGANGEPGKDGDTGPQGPAGAKGESG 573          

HSP 10 Score: 56.6102 bits (135), Expect = 1.152e-7
Identity = 46/101 (45.54%), Postives = 56/101 (55.45%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G  G +GAKG +G  G+ G  G  G  G KGE G+ G RG  G+AG  G+ G  GE GP G  G +G  G+ GPPG    PG  G  GP G +G DG+
Sbjct:  537 GMPGPSGVAGAKGANGEPGKDGDTGPQGPAGAKGESGLAGSRGMPGVAGKNGNAGEPGERGPVGPQGPQGAPGTQGPPGL---PGKTGESGPRGERGTDGK 634          

HSP 11 Score: 56.225 bits (134), Expect = 1.459e-7
Identity = 228/569 (40.07%), Postives = 264/569 (46.40%), Query Frame = 2
Query: 2498 GTRGPS---------GLPGKPGIDGKEGSIGPKGD------LGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL---GKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGR----------------------IGE---AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPG------------TIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G+ GP+         G PGK G  G+ G +GP GD       GPRG  G  GP G +G +G  G  G  GSKGD+G  G  G  G  G RG PG  G PG  G  G  G  G  G  G  G       QG  G  G  G+ G KG+ G  G DG+ G  GP    G++GL G+RG  G+ G  G  G  G RGP G  G  G+PG  G     G  GK G  GP G  G  G PG  G RG +G  G  GL GPTG PG                       IGE    GP G  G  G +G PG  G +G  G  G RGE+G  GP G  G  G  G  G  G  G  G +G +G  G+ G  G  G+ G+ GP GLPG  G  G +G+ GA GP G  G  G  G  G+ G+ G +G  G  G  G +G  G +G  G PG  G  G  GP G  G  GP GP G  GP G  G  G  G  G  G  G+ GEPG+ G     G  G PG             IG  G KG  GPPG+TG +G  G+PG KGE G  G  G  GE G
Sbjct:  405 GSEGPNGSPGPRGAPGPPGKRGKIGRVGPMGPIGDPGSRGATGPRGFPGAAGPNGRKGEQGSPGAAGFPGSKGDKGSSGKNGEPGRVGLRGPPGQPGSPGREGKTGLPGDDGQDGKPGAPGKPDRLSLQGMTGMPGPSGVAGAKGANGEPGKDGDTGPQGPAGAKGESGLAGSRGMPGVAGKNGNAGEPGERGPVGPQGPQGAPGTQG---PPGLPGKTGESGPRGERGTDGKPGAMGERGPQGETGPTGLTGPTGKPGDRGSNGADGTPVSKHEFIRYFAIIGEPGAQGPSGNPGTPGRQGMPGASGRRGAPGAKGSRGERGRVGPPGIDGKDGAPGSDGLNGPPGPQGPAGEKGEAGQPGTNGFNGKNGDQGPRGLPGTPGAPGRNGEQGASGPTGPVGPRGPGGIKGAKGVMGSSGRPGITGTLGQRGRLGSKGGEGTPGSQGMPGMSGPPGRPGPPGPPGPPGPSGPSGSNGRNGVKGSTGEGGRPGRDGEPGEPGTPGRDGEPGIPGPDGRPGERGPGGEIGRKGEKGPSGPPGITGNRGPPGSPGIKGERGYPGKDGRAGEPG 970          

HSP 12 Score: 52.373 bits (124), Expect = 1.960e-6
Identity = 52/134 (38.81%), Postives = 63/134 (47.01%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEV---------GMKGVKGEYGIQGPRGPEG-----------------IAGVKGDIGIQGET------GPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
             KG RG  G  G  GD GPSG+ G  G +         GM+G  G  G QGP+GP G                  AG+KG  G  G        GPRG DG +G  G VG PG +G+ G +G+ GP G+ G DG
Sbjct:   71 VKGQRGPDGPPGDIGDQGPSGEAGKPGPLGYNGQPGNQGMRGDPGPQGDQGPQGPPGGSTQAAGGIKGPDPNDLAAGIKGSNGAPGPRGSPGLPGPRGSDGPRGQRGEVGLPGESGSAGRRGASGPPGSPGIDG 204          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Nematostella
Match: EDO49368 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG67])

HSP 1 Score: 129.028 bits (323), Expect = 1.836e-30
Identity = 219/734 (29.84%), Postives = 294/734 (40.05%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGV---EGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLD-GKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRS---TRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMY-VDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCFS 5014
            +GV G  G +G  G +GP G++G+ GP G  G KG  G+       G  G++G+ G  G +G QGVSGE G+ G  G  G                                G+ GD G  GP G  G  G  GPPG  G  GA G PG  G +G  G  G +G++G               G G  G  G  G  GPQG    +G+ G QG  G  GE+GP G PGP G  G SG+ G                  + G PG+ G QG +G SG  G  G  G  G PG+ G  G++G  G  G  GP G          +         G R  D G  GE G+ G   L G++G  G  G  G+ GEPG  G  G  G  G PG  G  G  G IG  G  G  G  GP G  GP GPPG  G PG            P     I      +   ++ S   T+ Q    ++  L   ++ + NKI   +   G  K   AR+C ++K+       G YYIDPNEGS  D+ EV C F+    TC+     +    +      GE  W         I+   D+   Y +D  QM FLR  +  + Q + + C  S  + DS                   ++I L     LG NE  +  ++          N     D   ++T        + T     LPIID  +  Y+ +  +   + +  +CF+
Sbjct:   12 QGVPGTKGDRGEPGAVGPRGQQGEEGPTGPAGPKGDAGKNGQDGRPGYDGAKGERGAPGKQGKQGVSGEKGNQGKRGPAGA-------------------------------GIPGDDGRQGPPGPQGSRGVTGPPGFNGDKGANGAPGRAGDRGAQGPQGQKGDKG---------------GAGARGLPGVAGTPGPQGERGNDGKPGEQGERGAVGEVGPTGSPGPAGQPGVSGEAG------------------ADGTPGERGQQGSSGASGLPGRPGSPGPAGAPGLKGPRGEKGAKGDRGSKGPKGGASGFPGKPGLPGNPGIPGIGGRDGDMGPAGEDGRPGLSGLPGLRGQSGPAGEKGANGEPGSAGSPGTPGAMGPPGDSGSKGAAGIIGEPGSPGPRGPIGPQGDPGPEGPPGPPGPPGPPVSVNKLSHCIPYIIFAITGDNNANKQMFEESVSSTKQQLICFLDVCLIAVINLLINKIVTYKEQEG-TKGKPARSCRNLKIDNPDKKSGYYYIDPNEGSPSDAEEVWCDFTTNA-TCVFPKNTEIITRSLVVNSNGEDTW---------ITENDDVKIDYKLDVVQMKFLRLMHKTAKQNMTYHCYNSRAWEDS-------------------RTIKL-----LGDNEEEITAKLR--------LNECQNKDNQWHKT-----VLDISTKQKDKLPIID--VAPYDLQDGAQFKIELGPVCFA 631          

HSP 2 Score: 87.0409 bits (214), Expect = 3.520e-17
Identity = 150/429 (34.97%), Postives = 188/429 (43.82%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGIL---------GPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIV 3748
            G PG +G  G+PG  G  G+ G++GP+G  G  G +G  GP+G  G  GQ G  G  G+KG+RG  G QG  G  GE+G+ G +GP G   P                             + G +G  G +GS G  GP G  GD G  G  G  G RG  G +G +G  G  G+RG  GV+G PG  G+ G +                           G  GE+G +G +G +GPTG+P   G AG PG +G +GA+G PG +G +G +G           GP G  GL                            GP+G +G  G +G  G KG  G  G PG  G  G  G  G  G  G  G AG+DG  G  GLPG  G  GPAG  G  GE G  G  G PG  G  G  G  G  GI+
Sbjct:    4 GAPGPQGSQGVPGTKGDRGEPGAVGPRGQQGEEGPTGPAGPKGDAGKNGQDGRPGYDGAKGERGAPGKQGKQGVSGEKGNQGKRGPAGAGIPG----------------------------DDGRQGPPGPQGSRGVTGPPGFNGDKGANGAPGRAGDRGAQGPQGQKGDKGGAGARGLPGVAGTPGPQGERGND---------------------------GKPGEQGERGAVGEVGPTGSP---GPAGQPGVSGEAGADGTPGERGQQGSSGASGLPGRPGSPGPAGAPGL---------------------------KGPRGEKGAKGDRGSKGPKG--GASGFPGKPGLPGNPGIPGIGGRDGDMGPAGEDGRPGLSGLPGLRGQSGPAGEKGANGEPGSAGSPGTPGAMGPPGDSGSKGAAGII 345          

HSP 3 Score: 60.8474 bits (146), Expect = 4.379e-9
Identity = 106/291 (36.43%), Postives = 133/291 (45.70%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGS------IGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ-GVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            +GPPG +G  G+ G PG +G +G+       G +G  GP+GQ G+ G  G RG  G +G  G  G +G+ GK G QG  G  GE                         G  G  G  G PG  G  G  G  G  G++GS G  G  G  G  GP G  GLKG RGE G KG        GS+GP+ G SG PG PG                       G  GIPG  G  G+ G  G+ G  G +G PG  G++GP G+ GA+   G+PG  G+ G  G +GP G+ G
Sbjct:  101 QGPPGPQGSRGVTGPPGFNGDKGANGAPGRAGDRGAQGPQGQKGDKGGAGARGLPGVAGTPGPQGERGNDGKPGEQGERGAVGE------------------------VGPTGSPGPAGQPGVSGEAGADGTPGERGQQGSSGASGLPGRPGSPGPAGAPGLKGPRGEKGAKGDR------GSKGPKGGASGFPGKPG---------------------LPGNPGIPGIGGRDGDMGPAGEDGRPGLSGLPGLRGQSGPAGEKGAN---GEPGSAGSPGTPGAMGPPGDSG 337          

HSP 4 Score: 59.6918 bits (143), Expect = 8.305e-9
Identity = 93/273 (34.07%), Postives = 112/273 (41.03%), Query Frame = 2
Query: 3245 GKTGASGAEGQPGVKGTKGDTG---ILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPG--IAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGN---PGPKGETGLMGPIGPVGEDG 4039
            GK+GA G +G  GV GTKGD G    +GPRG+                                 QG EG  G  GP G  G+ G  G PG  G  G                          G PGK G QG        GE G +G RGP G  I G+ G++GP GP G                    G  GP G  G  G  G PG+ G+R  +G +G  G  G  G++G PG  G  GPQG+RGN   PG +GE G +G +GP G  G
Sbjct:    1 GKSGAPGPQGSQGVPGTKGDRGEPGAVGPRGQ---------------------------------QGEEGPTGPAGPKGDAGKNGQDGRPGYDGAKGER------------------------GAPGKQGKQG------VSGEKGNQGKRGPAGAGIPGDDGRQGPPGPQG------------------SRGVTGPPGFNGDKGANGAPGRAGDRGAQGPQGQKGDKGGAGARGLPGVAGTPGPQGERGNDGKPGEQGERGAVGEVGPTGSPG 192          

HSP 5 Score: 59.6918 bits (143), Expect = 1.028e-8
Identity = 49/121 (40.50%), Postives = 65/121 (53.72%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKG--VKGEYGIQGPRGPEGIAGVKGDIGIQGE---------------TGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            Y GA+G RG+ G +G  G SG+ G +G+ G  G  + G+ G QGP GP+G  GV G  G  G+                GP+G+ G+KG  G+ G PG AG PG +G RG  G  G  GE+
Sbjct:   59 YDGAKGERGAPGKQGKQGVSGEKGNQGKRGPAGAGIPGDDGRQGPPGPQGSRGVTGPPGFNGDKGANGAPGRAGDRGAQGPQGQKGDKGGAGARGLPGVAGTPGPQGERGNDGKPGEQGER 179          

HSP 6 Score: 58.9214 bits (141), Expect = 1.575e-8
Identity = 104/312 (33.33%), Postives = 127/312 (40.71%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLG---------PRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP----TGAPG---------------RIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQG 3337
            +G  G RGP+G  G PG DG++G  GP+G  G          +G +G PG  G RGA+G  G  G+ G  G RG  G  G  G +GER                                          G  G  G  G +G+VG +GP G     GP G+ G+    GE G  G+ G+ G  GS G  G+ G PGSPG AG                         PG  GPRGEKG KGD G  GP    +G PG               R G+ GP G+ G  G  G PG++G  G  G  G  GE G
Sbjct:   81 KGNQGKRGPAGA-GIPGDDGRQGPPGPQGSRGVTGPPGFNGDKGANGAPGRAGDRGAQGPQGQKGDKGGAGARGLPGVAGTPGPQGER------------------------------------------GNDGKPGEQGERGAVGEVGPTGSP---GPAGQPGVS---GEAGADGTPGERGQQGSSGASGLPGRPGSPGPAGA------------------------PGLKGPRGEKGAKGDRGSKGPKGGASGFPGKPGLPGNPGIPGIGGRDGDMGPAGEDGRPGLSGLPGLRGQSGPAGEKGANGEPG 319          

HSP 7 Score: 56.6102 bits (135), Expect = 7.190e-8
Identity = 47/105 (44.76%), Postives = 55/105 (52.38%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDE---GSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GARG+ G +G  G  G  G  G  GE G +G  GE G  G  GP G  GV G+ G  G  G RG+ G+ G     G  G PG AG PG+KG RG  G +G  G K
Sbjct:  150 GARGLPGVAGTPGPQGERGNDGKPGEQGERGAVGEVGPTGSPGPAGQPGVSGEAGADGTPGERGQQGSSGASGLPGRPGSPGPAGAPGLKGPRGEKGAKGDRGSK 254          

HSP 8 Score: 55.8398 bits (133), Expect = 1.447e-7
Identity = 43/101 (42.57%), Postives = 57/101 (56.44%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G+RG+ G  G  GD G +G  G  G+ G +G +G+ G +G  G  G+ GV G  G QGE G  GK G +G+ G+VG  G  G+PG  G  G  G  G DG
Sbjct:  107 QGSRGVTGPPGFNGDKGANGAPGRAGDRGAQGPQGQKGDKGGAGARGLPGVAGTPGPQGERGNDGKPGEQGERGAVGEVGPTGSPGPAGQPGVSGEAGADG 207          

HSP 9 Score: 53.9138 bits (128), Expect = 6.059e-7
Identity = 49/109 (44.95%), Postives = 57/109 (52.29%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGD---IGIQGETGPRGKDGNKGDEGSVGP----PGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G RG  G  G  G  GP+GQ G  GE G  G  GE G QG  G  G+ G  G     G  G  GPRG+ G KGD GS GP     G+ G PG+ G+ G  G  GRDG+
Sbjct:  176 QGERGAVGEVGPTGSPGPAGQPGVSGEAGADGTPGERGQQGSSGASGLPGRPGSPGPAGAPGLKGPRGEKGAKGDRGSKGPKGGASGFPGKPGLPGNPGIPGIGGRDGD 284          

HSP 10 Score: 51.2174 bits (121), Expect = 4.121e-6
Identity = 42/101 (41.58%), Postives = 52/101 (51.49%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGP---RGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            KGA G  G +G +G  GP GQ G +G  G +G+ G  G  GP   RG +G  G +G+ G  GE GP G  G  G  G  G  G  G PG +G +G  G  G
Sbjct:  122 KGANGAPGRAGDRGAQGPQGQKGDKGGAGARGLPGVAGTPGPQGERGNDGKPGEQGERGAVGEVGPTGSPGPAGQPGVSGEAGADGTPGERGQQGSSGASG 222          

HSP 11 Score: 50.8322 bits (120), Expect = 4.682e-6
Identity = 43/94 (45.74%), Postives = 51/94 (54.26%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G SGA G  G  G  GT+G+ G  G  G  G QG  GP G AG KGD G  G+ G  G DG KG+ G+   PG  G  G+ G +   GNQG+ G
Sbjct:    1 GKSGAPGPQGSQGVPGTKGDRGEPGAVGPRGQQGEEGPTGPAGPKGDAGKNGQDGRPGYDGAKGERGA---PGKQGKQGVSGEK---GNQGKRG 88          

HSP 12 Score: 50.8322 bits (120), Expect = 5.275e-6
Identity = 44/101 (43.56%), Postives = 53/101 (52.48%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G  G +G  G  G  GP GQ G  G  G  G KG+ G  G  G  G  G KG+ G  G+ G +G  G KG++G  GP G AG PG  G +GP G QG  G
Sbjct:   12 QGVPGTKGDRGEPGAVGPRGQQGEEGPTGPAGPKGDAGKNGQDGRPGYDGAKGERGAPGKQGKQGVSGEKGNQGKRGPAG-AGIPGDDGRQGPPGPQGSRG 111          

HSP 13 Score: 50.447 bits (119), Expect = 6.928e-6
Identity = 81/194 (41.75%), Postives = 93/194 (47.94%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAG 3067
            RG  G  GP+G PG  G  G  G  G  G  G RGQ G+ G  G+ G  G  GP G  G KG RG+ G +G  G KG +            G +G  G  G PG+ G  G+ G  G  GP GE G  GL G  G  G  GP GEKG  G  G  G  GT G +G  G        GS+G  G+ GEPGSPG  G
Sbjct:  179 RGAVGEVGPTGSPGPAGQPGVSGEAGADGTPGERGQQGSSGASGLPGRPGSPGPAGAPGLKGPRGEKGAKGDRGSKGPK-----------GGASGFPGKPGLPGNPGIPGIGGRDGDMGPAGEDGRPGLSGLPGLRGQSGPAGEKGANGEPGSAGSPGTPGAMGPPGDS------GSKGAAGIIGEPGSPGPRG 355          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Nematostella
Match: EDO42241 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S288])

HSP 1 Score: 103.219 bits (256), Expect = 6.082e-22
Identity = 207/552 (37.50%), Postives = 250/552 (45.29%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGP------TGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEV---------GIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGT---------KGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGV---------EGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG +GP GL G+P   G+ G+ GP+GD G  G+ G  GP G  GA G +GP       G +GS G +G+ GPQG  G KG+                                                RGL+GR+G  G +G  GEKG  GP G  G  GT G           G +GS G  G  G RGP G  G  G PG AG  G  G+ G QG  G  G  G  G+ G+ G  G+KG +G+ G  GP G PG  G  G PG+ GA GA G+P    +         KGD G  GP+G QG +G  G  GS G +GG+G  G +G  G  G  GV          GE G  G  G KG+ G  GLPG  G  GP G  G  GPPG  G+ G  G  G  G  G+ G QG AG  G+ G  G++GD+G PG  GN G  G  G  G                         +G  G  G+ G PG+ G    +G+ G PG  G  G +GEPG  G  G  GKRG PG +GE G  G  G  GE G 
Sbjct:  157 RGTPGAKGPPGLAGEP---GEPGTQGPRGDPGDVGEPGEAGPSGRNGANGTAGPPGVRGQPGAIGSPGPKGQQGPQGAEGFKGD------------------------------------------------RGLIGRRGPKGNLGEKGEKGAEGPGGVEGKNGTDGAAGPAGEPGADGPRGSPGALGDQGERGPAGAPGPQGLPGTAGPPGLPGDGGVQGDPGIEGESGPTGLAGRNGAGGDKGARGENGPAGPQGRPGAAGRQGDPGRDGAPGAPGKPVSTASNSESPDHIAKGDAGRAGPQGPQGAEGRTGRRGSLGERGGKGPQGSRGPVGPRGANGVPGSPGADGDTGEAGEHGADGLKGDRGADGLPGEDGIPGPRGPSGPTGPPGPDGKDGQKGEAGRDGENGQIGPQGRAGAPGSPGLGGQKGDQGMPGKPGNPGAAGLRGAPG------------------------ARGDYGERGEPGAPGRDGSPGQRGVPGVPGIPGLAGMQGEPGAQGPMGADGKRGAPGAQGERGPRGEQGKQGEKGH 633          

HSP 2 Score: 77.7962 bits (190), Expect = 4.037e-14
Identity = 187/554 (33.75%), Postives = 230/554 (41.52%), Query Frame = 2
Query: 2516 GLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGV---SGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGL---IGPTGAPGRI---GEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGI---------------------------AGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGE---PGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLM------GPIGPVGEDGE 4042
            G+PG PG  G  G+ G KG  G  G+ G PG QG RG  G  G  GE G  G  G  G  GP G +G+                                              G  G  G KG  GP G +G KGD G +G+ G KG  GE G KG+EG  G+ G  G  G    +GEPG+ G  G  G  G+QG++G  G  GP G+ G  G PG  G+ GV+GD G+    GPTG  GR    G+ G  G+ G +G +G+PG  G +GD G  G  G  G         S          G  G  G  GPQG EG  G +G  G +G  GP G  GPVG  G +                        G+PG  G  G  G +G  G  G +GDRG  G+                            G  G+ G NG IG                  P G  G  GS GL G+ G+   PG+ G     G++G+PG  G  G +GEPG PG  G  G+RG PG  G  GL       G  GP+G DG+
Sbjct:  146 GIPGLPGKRGDRGTPGAKGPPGLAGEPGEPGTQGPRGDPGDVGEPGEAGPSGRNGANGTAGPPGVRGQ---------------------------------------------PGAIGSPGPKGQQGPQGAEGFKGDRGLIGRRGPKGNLGEKGEKGAEGPGGVEGKNGTDGAAGPAGEPGADGPRGSPGALGDQGERGPAGAPGPQGLPGTAGPPGLPGDGGVQGDPGIEGESGPTGLAGRNGAGGDKGARGENGPAGPQGRPGAAGRQGDPGRDGAPGAPG---KPVSTASNSESPDHIAKGDAGRAGPQGPQGAEGRTGRRGSLGERGGKGPQGSRGPVGPRGAN------------------------GVPGSPGADGDTGEAGEHGADGLKGDRGADGLPGEDGIPGPRGPSGPTGPPGPDGKDGQKGEAGRDGENGQIG------------------PQGRAGAPGSPGLGGQKGDQGMPGKPGNPGAAGLRGAPGARGDYGERGEPGAPGRDGSPGQRGVPGVPGIPGLAGMQGEPGAQGPMGADGK 609          

HSP 3 Score: 72.4034 bits (176), Expect = 1.667e-12
Identity = 180/510 (35.29%), Postives = 217/510 (42.55%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGA------------------RGQSGPTGELG------SKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT 3955
            GT G +G  G+PG DG  GS G  GD G RG +G PGPQG+ G                    G+SGPTG  G       KG RG+ GP GP G  G  G  G  G  G  G  G      S  +       G+ G  GP G +G  G  GR+GS+G  G  G +G  GP+G  G  G  G  G  G  G+ G  G+ G +G  G  G PG+ G+ G +G  G  G  GP G  G +G  G+ G  G+ G +G  G  G  G  G+ G+ G PGK G  GA G  G  G +GD G  G  G  G D                           G  G  G  G+ G PG  G  G  G  GP+G  G                          G PG  G +GP G  G QGE G  G+ G  G  G  G++GP GP+G              GP+G  G+PG  G  G  G  GEPGQ+G R  +G  G  G  G  G  G PG  GLT
Sbjct:  269 GTDGAAGPAGEPGADGPRGSPGALGDQGERGPAGAPGPQGLPGTAGPPGLPGDGGVQGDPGIEGESGPTGLAGRNGAGGDKGARGENGPAGPQGRPGAAGRQGDPGRDGAPGAPGKPVSTASNSESPDHIAKGDAGRAGPQGPQGAEGRTGRRGSLGERGGKGPQGSRGPVGPRGANGVPGSPGADGDTGEAGEHGADGLKGDRGADGLPGEDGIPGPRGPSGPTGPPGPDGKDGQKGEAGRDGENGQIGPQGRAGAPGSPGLGGQKGDQGMPGKPGNPGAAGLRGAPGARGDYGERGEPGAPGRD---------------------------GSPGQRGVPGVPGIPGLAGMQGEPGAQGPMGADGKR------------------------GAPGAQGERGPRGEQGKQGEKGHAGEGGADGAPGIPGEQGPMGPVGA------------PGPVGNAGSPGSPGPSGPKGDSGEPGQQGPRGTQGSDGKRGANGNPGIPGVPGDAGLT 715          

HSP 4 Score: 67.0106 bits (162), Expect = 7.118e-11
Identity = 167/473 (35.31%), Postives = 209/473 (44.19%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGK---------------PGIDGKEGSIGPKG------DLGPRGQSGNPGPQGIRGARGQSGPTGELGS---------------------KGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPD---------------------GEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMG 3715
            RGP G  GP GLPG                PGI+G+ G  G  G      D G RG++G  GPQG  GA G+ G  G  G+                     KGD G+ GPQGP G +G  G  GS G  G  GP G  G  G  G  G  G  G  G  G  GE G  GL G +G+ G  G D                     G+KG+ G  G+ G  G +G  G  GS    GL G +G QG+ G+PG+PG AG+ G                                G +GD G  G  GAPGR G    PG+ G  G  G PG+ G +G+ G  GP    G DG  G  G++G +G RG+ G+QG  G++G  G +G  GI   PG +G +GP+G PGPVG +G  G  G  GP                G  G+ G QGP G  G+ G+ G  G+ G PG+ G+ G
Sbjct:  298 RGPAGAPGPQGLPGTAGPPGLPGDGGVQGDPGIEGESGPTGLAGRNGAGGDKGARGENGPAGPQGRPGAAGRQGDPGRDGAPGAPGKPVSTASNSESPDHIAKGDAGRAGPQGPQGAEGRTGRRGSLGERGGKGPQGSRGPVGPRGANGVPGSPGADGDTGEAGEHGADGLKGDRGADGLPGEDGIPGPRGPSGPTGPPGPDGKDGQKGEAGRDGENGQIGPQGRAGAPGS---PGLGGQKGDQGMPGKPGNPGAAGLRGA------------------------------PGARGDYGERGEPGAPGRDGS---PGQRGVPGVPGIPGLAGMQGEPGAQGP---MGADGKRGAPGAQGERGPRGEQGKQGEKGHAGEGGADGAPGI---PGEQGPMGPVGAPGPVGNAGSPGSPGPSGPK---------------GDSGEPGQQGPRGTQGSDGKRGANGNPGIPGVPGDAG 713          

HSP 5 Score: 62.3882 bits (150), Expect = 1.668e-9
Identity = 140/385 (36.36%), Postives = 167/385 (43.38%), Query Frame = 2
Query: 2936 LGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGD---------AGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXX---XSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGED 4036
            +G  GL G RG+ G  G++G  GL G  G  G  G  G PGD         +G NG  G  G  G +G  G +G  G  GQ GP+G +G KGD GLIG  G  G +GE       G  GAEG  GV+G  G  G  GP GE G D                           GP+G  G LG QG  G  G  GP GLPG                 G  G  GD G  G PG+ G++G  G AG +G  G+ G RG+ GP      PG AG  G  G +G  G  G                 G+ G     G +G +G+ G  G  GER  KG +GS G +G  G+ G PG PG  G  G+ G  G  G  G  G  G  GED
Sbjct:  145 VGIPGLPGKRGDRGTPGAKGPPGLAGEPGEPGTQGPRGDPGDVGEPGEAGPSGRNGANGTAGPPGVRGQPGAIGSPGPKGQQGPQGAEGFKGDRGLIGRRGPKGNLGE------KGEKGAEGPGGVEGKNGTDGAAGPAGEPGAD---------------------------GPRGSPGALGDQGERGPAGAPGPQGLPG---------------TAGPPGLPGDGGVQGDPGIEGESGPTGLAGRNGAGGDKGARGENGPAGPQGRPGAAGRQGDPGRDGAPGAPGKPVSTASNSESPDHIAKGDAGRAGPQGPQGAEGRTGRRGSLGERGGKGPQGSRGPVGPRGANGVPGSPGADGDTGEAGEHGADGLKGDRGADGLPGED 481          

HSP 6 Score: 60.077 bits (144), Expect = 9.804e-9
Identity = 46/109 (42.20%), Postives = 61/109 (55.96%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGI------QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G RG  G  G  G+ GPSG+ G  G  G  GV+G+ G       +G +GP+G  G KGD G+ G  GP+G  G KG++G+ GP G  G  G  G+ GP G  G DG +
Sbjct:  178 QGPRGDPGDVGEPGEAGPSGRNGANGTAGPPGVRGQPGAIGSPGPKGQQGPQGAEGFKGDRGLIGRRGPKGNLGEKGEKGAEGPGGVEGKNGTDGAAGPAGEPGADGPR 286          

HSP 7 Score: 59.6918 bits (143), Expect = 1.263e-8
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGT---------RGEVGMKGVKGEYGIQG---PRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVG 2113
            GA G+RG+ GA+GD+G  G+ G          RG  G+ G+ G  G+QG    +GP G  G +G  G QGE GPRG+ G +G++G  G  G  G PGI G +GP+G
Sbjct:  548 GAAGLRGAPGARGDYGERGEPGAPGRDGSPGQRGVPGVPGIPGLAGMQGEPGAQGPMGADGKRGAPGAQGERGPRGEQGKQGEKGHAGEGGADGAPGIPGEQGPMG 653          

HSP 8 Score: 55.0694 bits (131), Expect = 3.441e-7
Identity = 43/102 (42.16%), Postives = 57/102 (55.88%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA G +G  GA+G  GP+G  GT G  G +G  G+ G  G +G +G AG  G +G++G  G  G  G  GD G  GP G AG  G +G+ G VG+ G  G +
Sbjct:  901 GAIGPQGPQGAQGSQGPAGSPGTDGIPGARGTPGQPGTAGVKGNKGYAGNPGTLGLEGLRGRTGAQGPIGDRGRSGPKGAAGEDGAEGAAGLVGDSGEKGYR 1002          

HSP 9 Score: 53.9138 bits (128), Expect = 6.870e-7
Identity = 86/284 (30.28%), Postives = 114/284 (40.14%), Query Frame = 2
Query: 2516 GLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGI---RGARGQSGPTGELGSKGDRGK------FGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGL 3340
            G PG  G+ G +G  GP GD G  G  G+PG  G+   RG RG+ G  G+ GS+G RG+       GP G TG +G+                                 +        +  +G  G  GR G+ G IGP G +G  G  G  G  GT G  G +G+ G+ G  G +G +G +G PG+ G                                     +G++G  G  GP G  GR   +GP G  G  GAEG  G+ G  G+ G  G  G QGL
Sbjct:  797 GRPGPVGLPGPQGPAGPDGDHGMDGAKGDPGAAGVNGLRGPRGKPGRNGKDGSRGKRGEDGRPGAVGPPGKTGPRGK-------------------------------PGLQFGKELMFVCWQGDPGEPGRDGTPGAIGPQGPQGAQGSQGPAGSPGTDGIPGARGTPGQPGTAGVKGNKGYAGNPGTLG------------------------------------LEGLRGRTGAQGPIGDRGR---SGPKGAAGEDGAEGAAGLVGDSGEKGYRGAPGRQGL 1010          

HSP 10 Score: 52.7582 bits (125), Expect = 1.505e-6
Identity = 47/105 (44.76%), Postives = 58/105 (55.24%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVG---TRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G RG RG+ GAKG  G +G+ G   T+G  G  G  GE G  GP G  G  G  G  G++G+ G  G  G KG +G  G  G+ G+ G+ G RGP GN G  GEK
Sbjct:  152 GKRGDRGTPGAKGPPGLAGEPGEPGTQGPRGDPGDVGEPGEAGPSGRNGANGTAGPPGVRGQPGAIGSPGPKGQQGPQGAEGFKGDRGLIGRRGPKGNLGEKGEK 256          

HSP 11 Score: 52.7582 bits (125), Expect = 1.596e-6
Identity = 81/187 (43.32%), Postives = 97/187 (51.87%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIG------PDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQ 3028
            RG PG  G  G  GK G DG+ G++GP G  GPRG+ G    + +     Q  P GE G  G  G  GPQGP G +G +G  GS G  G  G  G  G  G+ G KG KG  GNPG        G+ GL GR G+ GPIG      P G  G+ G  G  GL G  GE G +G+ G+ GL G RGP+
Sbjct:  838 RGKPGRNGKDGSRGKRGEDGRPGAVGPPGKTGPRGKPGLQFGKELMFVCWQGDP-GEPGRDGTPGAIGPQGPQGAQGSQGPAGSPGTDGIPGARGTPGQPGTAGVKGNKGYAGNPGTL------GLEGLRGRTGAQGPIGDRGRSGPKGAAGEDGAEGAAGLVGDSGEKGYRGAPGRQGLQGKRGPE 1017          

HSP 12 Score: 52.373 bits (124), Expect = 1.874e-6
Identity = 44/106 (41.51%), Postives = 60/106 (56.60%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNK---GDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +GA+G +G +G+ G  G  G  GT G+ G  GVKG  G  G  G  G+ G++G  G QG  G RG+ G K   G++G+ G  G  G+ G KG RG  G QG  G++
Sbjct:  909 QGAQGSQGPAGSPGTDGIPGARGTPGQPGTAGVKGNKGYAGNPGTLGLEGLRGRTGAQGPIGDRGRSGPKGAAGEDGAEGAAGLVGDSGEKGYRGAPGRQGLQGKR 1014          

HSP 13 Score: 52.373 bits (124), Expect = 2.294e-6
Identity = 43/97 (44.33%), Postives = 57/97 (58.76%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G+RG  GA G  GP GQ G +   G +G KG+ G+ G RGP+G  G KG+   +G  GP G +G  G +G+ GP G  G  G +GS G +G+QG  G
Sbjct:  209 GVRGQPGAIGSPGPKGQQGPQ---GAEGFKGDRGLIGRRGPKGNLGEKGE---KGAEGPGGVEGKNGTDGAAGPAGEPGADGPRGSPGALGDQGERG 299          

HSP 14 Score: 51.2174 bits (121), Expect = 4.316e-6
Identity = 71/212 (33.49%), Postives = 86/212 (40.57%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRG------------EVGIKGSE------GKTGLMGSRGPQGVSGEPGSPGDAGV 3070
            G PG  GP G  G  G  G  GS G  G  G RG  G PG  G++G +G +G  G LG +G RG+ G QGP GD+G                                            G KG  G  G +G+ G +G  GEKG  G  G+ GL+G RG            E G KG +      G  G  G+RGP G  G+ G PG  G 
Sbjct:  898 GTPGAIGPQGPQGAQGSQGPAGSPGTDGIPGARGTPGQPGTAGVKGNKGYAGNPGTLGLEGLRGRTGAQGPIGDRGR------------------------------------------SGPKGAAGEDGAEGAAGLVGDSGEKGYRGAPGRQGLQGKRGPEGDPGSPGGSGEAGDKGVDGPPGSPGPQGFNGARGPNGPKGDSGRPGPRGF 1067          

HSP 15 Score: 50.8322 bits (120), Expect = 5.329e-6
Identity = 49/122 (40.16%), Postives = 59/122 (48.36%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP---------PGYA------------GNPGIKGSRGPVGNQGRDG 2131
            KG +G +G+ G KGD G  G+ G +G +G KG KG  G  G  G  G  G  G  G  G  GPRG  G  GD+G  GP         PG A            G+PGI+G  GP G  GR+G
Sbjct:  223 KGQQGPQGAEGFKGDRGLIGRRGPKGNLGEKGEKGAEGPGGVEGKNGTDGAAGPAGEPGADGPRGSPGALGDQGERGPAGAPGPQGLPGTAGPPGLPGDGGVQGDPGIEGESGPTGLAGRNG 344          

HSP 16 Score: 50.0618 bits (118), Expect = 9.614e-6
Identity = 48/131 (36.64%), Postives = 60/131 (45.80%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMK--------------------GVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGI---------KGSRGPVGNQGRDGEK 2137
            G+RG RG  G  G  GP G+ G RG+ G++                    G  G  G QGP+G +G  G  G  G  G  G RG  G  G  G  G  GYAGNPG           G++GP+G++GR G K
Sbjct:  848 GSRGKRGEDGRPGAVGPPGKTGPRGKPGLQFGKELMFVCWQGDPGEPGRDGTPGAIGPQGPQGAQGSQGPAGSPGTDGIPGARGTPGQPGTAGVKGNKGYAGNPGTLGLEGLRGRTGAQGPIGDRGRSGPK 978          

HSP 17 Score: 50.0618 bits (118), Expect = 9.696e-6
Identity = 44/99 (44.44%), Postives = 52/99 (52.53%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GI G  G +GD G     G +G  G+ G  GE G QGPRG        GD+G  GE GP G++G  G   + GPPG  G PG  GS GP G QG  G +
Sbjct:  146 GIPGLPGKRGDRG---TPGAKGPPGLAGEPGEPGTQGPRG------DPGDVGEPGEAGPSGRNGANG---TAGPPGVRGQPGAIGSPGPKGQQGPQGAE 232          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Nematostella
Match: EDO30432 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0T5])

HSP 1 Score: 97.8265 bits (242), Expect = 3.450e-21
Identity = 179/514 (34.82%), Postives = 217/514 (42.22%), Query Frame = 2
Query: 2519 LPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGT---RGEVGIKGSEGKT---GLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
             PG  G+ G  G+ G KG  G RG  G PG +G RG +G  G  G  G  G  GK GP+G TG +G                                              KG+RGL G KG +GP G  G +G +GP G  GLKGT   RG  G+KGS G T   G  G+RGPQG+ G  G  G  G+                               G +G+KG  G  GP G  GR G  G PGK   SG  G PG  G  G  G +GPRG +GL G  G  G++G +G              G  G  G  G+ G PG KG+ G  G  GP G  G                          G  G  GL+G  G  G QG  G+ G +G  G  G  G RG  GP G  G  G +G  G  G  GP G  G +G RG+ G+ G+ G  G+    G++G  G  G  G  G  GP GL G +G RG  GP+G+TG  GP G  G  G+
Sbjct:   17 FPGPRGLRGYTGARGLKGARGYRGWRGLPGQRGYRGLKGAKGQRGLRGLTGATGKQGPRGWTGARGP---------------------------------------------KGIRGLRGLKGPIGPRGLKGSRGFMGPRGLRGLKGTTGSRGYRGLKGSRGLTGPQGKTGARGPQGLRGYRGFTGKPGLT------------------------------GPRGLKGARGATGPRGYTGRTGLRGVPGK---SGRNGTPGKNGRPGAKGAMGPRGYRGLTGSKGARGARGKQGL------------RGATGRPGRNGVPGRPGSKGKTGARGATGPRGLRGLR------------------------GPQGNQGLRGLKGSRGVQGRPGKNGAKGRNGAPGRTGARGKIGPRGFTGLKGAKGVRGATGLRGPRGFTGKQGLRGVPGRTGKTGANGK---PGLRGPQGLKGLKGKTGATGPRGLRGLKGSRGWTGPRGKTGATGPQGLRGYRGK 413          

HSP 2 Score: 94.3597 bits (233), Expect = 4.335e-20
Identity = 170/501 (33.93%), Postives = 199/501 (39.72%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGK------TGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGK 3970
            RG PG RG  GL G  G  G  G  G  G  GPRG +G  GP+GIRG RG  GP G  G KG RG  GP+G  G KG                                      G +G  G KG RGL G +G  G  GP G +G  G  GK       GLKG RG  G +G  G+TGL G  G  G +G PG  G  G                             G  G +G +G  G  G  GA G+ G  G  G+ G +G  G+PG KG  G  G  GPRG +GL                      G  GN G +G++G  G+QG PG+ G  G  G PG  G              G  G  G  G  G+ G+ G  GL+GP G +G QG  G  G  G  G  G  G RGP                                  GL G  G+ G  G R L+G+KGS G  G  G  G  GP GL G +GK
Sbjct:   42 RGLPGQRGYRGLKGAKGQRGLRGLTGATGKQGPRGWTGARGPKGIRGLRGLKGPIGPRGLKGSRGFMGPRGLRGLKGT------------------------------------TGSRGYRGLKGSRGLTGPQGKTGARGPQGLRGYRGFTGKPGLTGPRGLKGARGATGPRGYTGRTGLRGVPGKSGRNGTPGKNGRPGAK---------------------------GAMGPRGYRGLTGSKGARGARGKQGLRGATGRPGRNGVPGRPGSKGKTGARGATGPRGLRGLR---------------------GPQGNQGLRGLKGSRGVQGRPGKNGAKGRNGAPGRTGA------------RGKIGPRGFTGLKGAKGVRGATGLRGPRGFTGKQGLRGVPGRTGKTGANGKPGLRGPQ---------------------------------GLKGLKGKTGATGPRGLRGLKGSRGWTGPRGKTGATGPQGLRGYRGK 413          

HSP 3 Score: 62.003 bits (149), Expect = 1.129e-9
Identity = 46/102 (45.10%), Postives = 60/102 (58.82%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G +GIRG  G KG  GP G  G+RG +G +G++G  G  G RG  G+ G +G  G QG+TG RG  G +G  G  G PG  G  G+KG+RG  G +G  G 
Sbjct:   81 RGPKGIRGLRGLKGPIGPRGLKGSRGFMGPRGLRGLKGTTGSRGYRGLKGSRGLTGPQGKTGARGPQGLRGYRGFTGKPGLTGPRGLKGARGATGPRGYTGR 182          

HSP 4 Score: 60.4622 bits (145), Expect = 3.828e-9
Identity = 49/121 (40.50%), Postives = 65/121 (53.72%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---------PEGIAGVKGDIGIQGE---------TGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG RG+RG +GA G  GP G  G RG  G++G++G  G  GPRG         P G+ G+KG  G +G          TGP+GK G +G +G  G  G+ G PG+ G RG  G +G  G +
Sbjct:   57 KGQRGLRGLTGATGKQGPRGWTGARGPKGIRGLRGLKGPIGPRGLKGSRGFMGPRGLRGLKGTTGSRGYRGLKGSRGLTGPQGKTGARGPQGLRGYRGFTGKPGLTGPRGLKGARGATGPR 177          

HSP 5 Score: 60.077 bits (144), Expect = 5.377e-9
Identity = 45/102 (44.12%), Postives = 57/102 (55.88%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G+RG RG  G++G  GP G+ G RG  G++G +G  G  G  GP G+ G +G  G +G TG  G  G  G  G  G PG  G PG KG+ GP G +G  G K
Sbjct:  121 GSRGYRGLKGSRGLTGPQGKTGARGPQGLRGYRGFTGKPGLTGPRGLKGARGATGPRGYTGRTGLRGVPGKSGRNGTPGKNGRPGAKGAMGPRGYRGLTGSK 222          

HSP 6 Score: 60.077 bits (144), Expect = 5.377e-9
Identity = 101/286 (35.31%), Postives = 129/286 (45.10%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGD---LGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGT---RGEVGIKGSEGKTGLMGSRGP------QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDT 3307
            RG PG  G +G PGK G  G +G++GP+G     G +G  G  G QG+RGA G+ G  G  G  G +GK G +G TG +G RG  G QG                                     +G+RGL G +G  G  G +G KG  G  G+TG +G    RG  G+KG++G  G  G RGP      QG+ G PG  G  G NG+ G +G QG                      KG+KG  G  GP G  G  G  G  G  G +GA G  G++G +G T
Sbjct:  186 RGVPGKSGRNGTPGKNGRPGAKGAMGPRGYRGLTGSKGARGARGKQGLRGATGRPGRNGVPGRPGSKGKTGARGATGPRGLRGLRGPQG------------------------------------NQGLRGLKGSRGVQGRPGKNGAKGRNGAPGRTGARGKIGPRGFTGLKGAKGVRGATGLRGPRGFTGKQGLRGVPGRTGKTGANGKPGLRGPQG---------------------LKGLKGKTGATGPRGLRGLKGSRGWTGPRGKTGATGPQGLRGYRGKT 414          

HSP 7 Score: 58.151 bits (139), Expect = 1.771e-8
Identity = 49/112 (43.75%), Postives = 60/112 (53.57%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY---------AGNPGIKGSRGPVGNQGRDGEK 2137
            KGARG RG  G  G  G  G  G +G+ G++G+ G  G QGPRG  G  G KG  G++G  GP G  G KG  G +GP G           G  G+KGSRG  G QG+ G +
Sbjct:   33 KGARGYRGWRGLPGQRGYRGLKGAKGQRGLRGLTGATGKQGPRGWTGARGPKGIRGLRGLKGPIGPRGLKGSRGFMGPRGLRGLKGTTGSRGYRGLKGSRGLTGPQGKTGAR 144          

HSP 8 Score: 56.9954 bits (136), Expect = 3.873e-8
Identity = 46/104 (44.23%), Postives = 57/104 (54.81%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGM------KGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            KG+RG+ G  G  G  GP G  G RG  G       +G+KG  G  GPRG  G  G++G  G  G  G  GK+G  G +G++GP GY G  G KG+RG  G QG
Sbjct:  129 KGSRGLTGPQGKTGARGPQGLRGYRGFTGKPGLTGPRGLKGARGATGPRGYTGRTGLRGVPGKSGRNGTPGKNGRPGAKGAMGPRGYRGLTGSKGARGARGKQG 232          

HSP 9 Score: 55.0694 bits (131), Expect = 1.527e-7
Identity = 46/107 (42.99%), Postives = 58/107 (54.21%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGP------RGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G RG++GS G  G  G  G  GT G  G +G+KG  G+ GP      RGP+G+ G +G  G  G TGPR   G KG  G+ GP GY G  G++G  G  G  G  G+
Sbjct:   97 GPRGLKGSRGFMGPRGLRGLKGTTGSRGYRGLKGSRGLTGPQGKTGARGPQGLRGYRGFTGKPGLTGPR---GLKGARGATGPRGYTGRTGLRGVPGKSGRNGTPGK 200          

HSP 10 Score: 54.6842 bits (130), Expect = 2.104e-7
Identity = 43/92 (46.74%), Postives = 54/92 (58.70%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            KGA+G+RG++G +G  G +G+ G RG  G  G  G  G  G RGP+G+ G+KG  G    TGPRG  G KG  G  GP G  G  G +G RG
Sbjct:  321 KGAKGVRGATGLRGPRGFTGKQGLRGVPGRTGKTGANGKPGLRGPQGLKGLKGKTGA---TGPRGLRGLKGSRGWTGPRGKTGATGPQGLRG 409          

HSP 11 Score: 53.1434 bits (126), Expect = 7.786e-7
Identity = 43/96 (44.79%), Postives = 54/96 (56.25%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  GAKG  GP G  G  G  G +G +G+ G++G  G  G  GV G  G +G+TG RG  G +G  G  GP G  G  G+KGSRG  G  G++G K
Sbjct:  202 GRPGAKGAMGPRGYRGLTGSKGARGARGKQGLRGATGRPGRNGVPGRPGSKGKTGARGATGPRGLRGLRGPQGNQGLRGLKGSRGVQGRPGKNGAK 297          

HSP 12 Score: 51.9878 bits (123), Expect = 1.530e-6
Identity = 45/110 (40.91%), Postives = 57/110 (51.82%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGET------------GPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G +G +GA G  G  G++G RG  G+KG KG  G  G RGP G  G +G  G+ G T            GP+G  G KG  G+ GP G     G+KGSRG  G +G+ G 
Sbjct:  295 GAKGRNGAPGRTGARGKIGPRGFTGLKGAKGVRGATGLRGPRGFTGKQGLRGVPGRTGKTGANGKPGLRGPQGLKGLKGKTGATGPRGLR---GLKGSRGWTGPRGKTGA 401          

HSP 13 Score: 50.447 bits (119), Expect = 5.223e-6
Identity = 44/113 (38.94%), Postives = 64/113 (56.64%), Query Frame = 2
Query: 1829 KGARGIRGSSGAK------GDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGP---EGIAGVKGDIGIQ---GETGPRGKD---GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            KGARG RG  G +      G +G  G+ G++G+ G +G  G  G++G RGP   +G+ G+KG  G+Q   G+ G +G++   G  G  G +GP G+ G  G KG RG  G +G
Sbjct:  222 KGARGARGKQGLRGATGRPGRNGVPGRPGSKGKTGARGATGPRGLRGLRGPQGNQGLRGLKGSRGVQGRPGKNGAKGRNGAPGRTGARGKIGPRGFTGLKGAKGVRGATGLRG 334          

HSP 14 Score: 49.6766 bits (117), Expect = 8.186e-6
Identity = 43/116 (37.07%), Postives = 63/116 (54.31%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG---IAGVKGDIGIQGE---------------TGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            +G +G+RG++G  G +G  G+ G++G+ G +G  G  G++G RGP+G   + G+KG  G+QG                TG RGK G +G  G  G  G  G  G++G RG  G QG
Sbjct:  228 RGKQGLRGATGRPGRNGVPGRPGSKGKTGARGATGPRGLRGLRGPQGNQGLRGLKGSRGVQGRPGKNGAKGRNGAPGRTGARGKIGPRGFTGLKGAKGVRGATGLRGPRGFTGKQG 343          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Medaka
Match: ENSORLT00000038463.1 (pep primary_assembly:ASM223467v1:6:10563937:10584228:1 gene:ENSORLG00000026346.1 transcript:ENSORLT00000038463.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 113.235 bits (282), Expect = 2.880e-25
Identity = 179/502 (35.66%), Postives = 223/502 (44.42%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKT---GLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGP---------------PGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPG 3949
            G +GP G PG  G +G  G +GPKG  G  G  G PGPQG +G  G  GP G++G KG +G  GPQG  G  G +G                                           KG  G  G KG  GP GP GE G  GP G +   G +G +G++G KG++G  G  G +G  G  G  G PG +G                                   G KGD+G  GPT   G +G  GP G  G+ G +G  G +G KG  G LGP+G+ G  GP       G KG  G  G QG  GN+G  G +GE+   GP G KG++GP GL G +G++GP G+ G PGP G  G  G  G  G+PG  G  G QGP G  G QG+ G+ G +GP               PG+ G++G  GP G +G+                           +GL G  G  GQKGE   KG KG  G +G  GS  EPGPPG
Sbjct:  112 GHQGPIGQPGIKGSEGPRGYMGPKGSNGIPGMCGTPGPQGPQGEIGAQGPKGKMGLKGAKGDIGPQGQKGQMGSKG------------------------------------------SKGEMGPKGLKGQTGPQGPKGETGPQGPPGPSENKGAQGPKGDIGPKGAKGAKGQQGPKGEMGEKGLKGLPGLSGT---------------------------------PGAKGDMGPQGPT---GDLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGDVGPQGPGTKGEMGGTKGAVGAMGPQGPQGNTGAMGAKGEM---GPNGLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQKGDTGPQGPKGNEGSQGPTGTPGNPGLKGDLGPPGPTGDMGL---------------------------QGLKGDKGAKGQKGEIGEKGTKGDLGPMGVKGSAAEPGPPG 505          

HSP 2 Score: 100.523 bits (249), Expect = 4.150e-21
Identity = 143/441 (32.43%), Postives = 187/441 (42.40%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGP------RGQSGNPGPQGIRGARGQSGPTGELGSK------------GDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDI---GLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGI 3745
            +G  G  GP GL G+ G  G +G  GP+G  GP      +G  G+ GP+G +GA+GQ GP GE+G K            G +G  GPQGPTGD G +                                    GP+G  G +G++G +G++G  G IGP G KGDVGP G     GT+GE+G  G++G  G MG +GPQG +G  G+ G+ G N                                 G+KGD+   GL G  G+ G  GE GPPG  G  G +G  G  G  G  G LGP+G +G                           + G QG +G+ G QGP G +G  GP G PG                              +PGL G  G  GP G  G QG  G++G +G  G  G  G +G  GP+G+
Sbjct:  186 KGSKGEMGPKGLKGQTGPQGPKGETGPQGPPGPSENKGAQGPKGDIGPKGAKGAKGQQGPKGEMGEKGLKGLPGLSGTPGAKGDMGPQGPTGDLGPQ------------------------------------GPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGDVGPQG----PGTKGEMG--GTKGAVGAMGPQGPQGNTGAMGAKGEMGPN---------------------------------GLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKG---------------------------DVGQQGQKGDTGPQGPKGNEGSQGPTGTPG------------------------------NPGLKGDLGPPGPTGDMGLQGLKGDKGAKGQKGEIGEKGTKGDLGPMGV 494          

HSP 3 Score: 96.6709 bits (239), Expect = 5.862e-20
Identity = 156/411 (37.96%), Postives = 193/411 (46.96%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSR---GPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE------RGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP------GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            KG  G+ G  G+ GP GP GE G  GP GK GLKG +G++G +G +G+ G  GS+   GP+G+ G+ G  G  G                       G  G PGP   KG +G  G IGP GA G  G+ GP G+ G  G +G PG+ GT G  G +GP+G  G                    G QG  G+ G QG++G++G QG  G  G LGP G  GP G                          G+ G  G  G QGP G +G  G  GE      +GD GP G+ GNMG  GP G IG  GP G  G  GP G  G  G  G  G +G  G VG+ GQKG+   +G KG+ G+ G  G+ G P      GPPG TG  G +G  G KG  G  G IG  G  G+
Sbjct:  135 KGSNGIPGMCGTPGPQGPQGEIGAQGPKGKMGLKGAKGDIGPQGQKGQMGSKGSKGEMGPKGLKGQTGPQGPKGET---------------------GPQGPPGPSENKGAQGPKGDIGPKGAKGAKGQQGPKGEMGEKGLKGLPGLSGTPGAKGDMGPQGPTG------------------DLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGDVGPQGP------------------GTKGEMGGTKGAVGAMGPQGPQGNTGAMGAKGEMGPNGLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQKGDTGPQGPKGNEGSQGPTGTPGNPGLKGDLGPPGPTGDMGLQGLKGDKGAKGQKGEIGEKGTKGD 488          

HSP 4 Score: 84.3445 bits (207), Expect = 5.036e-16
Identity = 138/377 (36.60%), Postives = 166/377 (44.03%), Query Frame = 2
Query: 2948 GLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGS------PGTIGAVGSKGEPGPP------GLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G +G  G+ GIKGSEG  G MG +G  G+ G  G+PG  G                                GE G +G  G +G  GA G IG  G  G+ G+ G++G+ G KG KG TG  GP+GE G          +G  G     G QG  G+ GP+G +G  G QGP G  GE G  GLPG  GT                           PG  G  G QGP G  G QG  G  G +G  G  G  G +G  GP+G  G  GP+G        G  G  G  G +G  G  G  G KGE    G+KG        G +G+ G KGE GPP      GL GP G  GNPG KG+ G  GP G VG+ G+
Sbjct:  112 GHQGPIGQPGIKGSEGPRGYMGPKGSNGIPGMCGTPGPQGPQ------------------------------GEIGAQGPKGKMGLKGAKGDIGPQGQKGQMGSKGSKGEMGPKGLKGQTGPQGPKGETG---------PQGPPGPSENKGAQGPKGDIGPKGAKGAKGQQGPKGEMGEKGLKGLPGLSGT---------------------------PGAKGDMGPQGPTGDLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGDVGPQGPGTKGEMGGTKGAVGAMGPQGPQGNTGAMGAKGEMGPNGLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQ 422          

HSP 5 Score: 65.855 bits (159), Expect = 2.994e-10
Identity = 61/164 (37.20%), Postives = 76/164 (46.34%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGS 2989
            G +GPSG PG PG  G  G  GPKGD+G +GQ G+ GPQG +G  G  GPTG  G+ G +G  GP GPTGD G +                                             G++G  G KG  G IG  G KGD+GP+   G+KG+  E G  G+
Sbjct:  391 GLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQKGDTGPQGPKGNEGSQGPTGTPGNPGLKGDLGPPGPTGDMGLQ---------------------------------------------GLKGDKGAKGQKGEIGEKGTKGDLGPM---GVKGSAAEPGPPGT 506          

HSP 6 Score: 65.4698 bits (158), Expect = 3.290e-10
Identity = 56/123 (45.53%), Postives = 66/123 (53.66%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSG------QVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGD---------EGSVGPPGYAGNPGIKGSRGP------VGNQGRDGEK 2137
            GA+G  G +G KGD GP G        G +GE+G  G KG  G+QGP G  G  G KGD+G QG  G  G+ G KGD         EGS GP G  GNPG+KG  GP      +G QG  G+K
Sbjct:  349 GAKGEMGPNGLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQKGDTGPQGPKGNEGSQGPTGTPGNPGLKGDLGPPGPTGDMGLQGLKGDK 471          

HSP 7 Score: 65.0846 bits (157), Expect = 5.189e-10
Identity = 97/282 (34.40%), Postives = 128/282 (45.39%), Query Frame = 2
Query: 3233 AGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGR------KGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPK------GETGLMGPIGPVGEDGE 4042
             GP G+ G  G  GQPG+KG++G  G +GP+G  G+                      G  G  GPQG +GE+G QGP G+      KG++GP G  G +G                           S G  G+ G +G  G +G QG  GE G +GPPG + N G +GP G IG                        PKG++G  G+ G  G+ GE+ LKG+ G  GT GA G  G  GP G  GPQG +G+ G +      G+ GL G IGP+G  G+
Sbjct:  105 PGPKGERGHQGPIGQPGIKGSEGPRGYMGPKGSNGIP---------------------GMCGTPGPQGPQGEIGAQGPKGKMGLKGAKGDIGPQGQKGQMG---------------------------SKGSKGEMGPKGLKGQTGPQGPKGETGPQGPPGPSENKGAQGPKGDIG------------------------PKGAKGAKGQQGPKGEMGEKGLKGLPGLSGTPGAKGDMGPQGPTGDLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGD 314          

HSP 8 Score: 63.929 bits (154), Expect = 1.204e-9
Identity = 106/291 (36.43%), Postives = 134/291 (46.05%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGP---IGPDG------------------EKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGT 3295
            +GP G  G  GL G PG+ G  G+   KGD+GP+G +G+ GPQG +G  G  G  G++G +G +G  GP GP GD G +G        G  G  G +G  G  G+ G  G  G  GP G  G+ G +GL G  GS GP   IGP G                  +KGD+GP G  G  G +G+ G  G +G  G  GS+GP G  G PG  GD G  G  G+ G                         +G+KGD G  G  G  G  G  G  G  G  G+  +PG  GT
Sbjct:  243 QGPKGEMGEKGLKGLPGLSGTPGA---KGDMGPQGPTGDLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGDVGPQGPGTKGEMGGTKGAVGAMGPQGPQGNTGAMGAKGEMGPNGLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQKGDTGPQGPKGNEGSQGPTGTPGNPGLKGDLGPPGPTGDMGL------------------------QGLKGDKGAKGQKGEIGEKGTKGDLGPMGVKGSAAEPGPPGT 506          

HSP 9 Score: 62.003 bits (149), Expect = 4.635e-9
Identity = 45/106 (42.45%), Postives = 63/106 (59.43%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGT---RGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KG+ GI G  G  G  GP G++G    +G++G+KG KG+ G QG +G  G  G KG++G +G  G  G  G KG+ G  GPPG + N G +G +G +G +G  G K
Sbjct:  135 KGSNGIPGMCGTPGPQGPQGEIGAQGPKGKMGLKGAKGDIGPQGQKGQMGSKGSKGEMGPKGLKGQTGPQGPKGETGPQGPPGPSENKGAQGPKGDIGPKGAKGAK 240          

HSP 10 Score: 61.6178 bits (148), Expect = 6.259e-9
Identity = 45/92 (48.91%), Postives = 56/92 (60.87%), Query Frame = 2
Query: 1847 RGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            +G+ G KGD GP G  G +G+ G KG  GE G++G  G  G  G KGD+G QG TG  G  G KG EGS G  G  G  G+KG+ GP+G +G
Sbjct:  222 KGAQGPKGDIGPKGAKGAKGQQGPKGEMGEKGLKGLPGLSGTPGAKGDMGPQGPTGDLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKG 313          

HSP 11 Score: 60.077 bits (144), Expect = 1.877e-8
Identity = 49/109 (44.95%), Postives = 66/109 (60.55%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVG-MKGVKGEYGIQGPRGPEGIAGVKGDIG---IQGETGPRGKDGNKGD---EGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G +G  G  G KGD GP G  GT+GE+G  KG  G  G QGP+G  G  G KG++G   ++G+ GP+G  GN G    +G +GPPG  GN G++G  G  GN G+ G+
Sbjct:  300 QGLKGAIGPLGPKGDVGPQG-PGTKGEMGGTKGAVGAMGPQGPQGNTGAMGAKGEMGPNGLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGD 407          

HSP 12 Score: 58.5362 bits (140), Expect = 4.861e-8
Identity = 45/103 (43.69%), Postives = 62/103 (60.19%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +G +G  G+ GAKG+ GP+G    +G++G +G+KG  G  GP+G  G  G KG+ G+QG +G  G  G KGD G  GP G  G  G KG  GP G +G +G +
Sbjct:  339 QGPQGNTGAMGAKGEMGPNG---LKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQKGDTGPQGPKGNEGSQ 438          

HSP 13 Score: 58.151 bits (139), Expect = 7.148e-8
Identity = 48/109 (44.04%), Postives = 59/109 (54.13%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSG------QVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
             +G  G  G+ G KGD GP G      Q G +G+ G +G KG  G QGP G  G  G+KGD+G  G TG  G  G KGD+G+ G  G  G  G KG  GP+G +G   E
Sbjct:  392 LQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQKGDTGPQGPKGNEGSQGPTGTPGNPGLKGDLGPPGPTGDMGLQGLKGDKGAKGQKGEIGEKGTKGDLGPMGVKGSAAE 500          

HSP 14 Score: 57.3806 bits (137), Expect = 1.024e-7
Identity = 48/104 (46.15%), Postives = 62/104 (59.62%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGP---EGIAGVKGDIGIQGETGPRGKDGNK---GDEGSVGPPGYAGNPGIKGSRGPVGNQG 2122
            +G +G  GS G+KG+ GP G    +G+ G +G KGE G QGP GP   +G  G KGDIG +G  G +G+ G K   G++G  G PG +G PG KG  GP G  G
Sbjct:  177 QGQKGQMGSKGSKGEMGPKG---LKGQTGPQGPKGETGPQGPPGPSENKGAQGPKGDIGPKGAKGAKGQQGPKGEMGEKGLKGLPGLSGTPGAKGDMGPQGPTG 277          

HSP 15 Score: 57.3806 bits (137), Expect = 1.079e-7
Identity = 42/98 (42.86%), Postives = 56/98 (57.14%), Query Frame = 2
Query: 1844 IRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            + G+ GA G  GP G  G  G +G KG  G  G++G  GP+G+ G  G  G +GE GP G  GN+G +G  G PG  G  G  G +GP G+ G+ G+K
Sbjct:  326 MGGTKGAVGAMGPQGPQGNTGAMGAKGEMGPNGLKGDMGPQGLKGNMGSTGPKGEIGPPGPKGNEGLQGPSGTPGNPGQKGDLGPQGPKGDVGQQGQK 423          

HSP 16 Score: 56.9954 bits (136), Expect = 1.713e-7
Identity = 45/110 (40.91%), Postives = 62/110 (56.36%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGV---------KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            +G +G  G  G KG  GP G +G +G  G+ G+         +GE G QGP+G  G+ G KGDIG QG+   +G+ G+KG +G +GP G  G  G +G +G  G QG  G
Sbjct:  111 RGHQGPIGQPGIKGSEGPRGYMGPKGSNGIPGMCGTPGPQGPQGEIGAQGPKGKMGLKGAKGDIGPQGQ---KGQMGSKGSKGEMGPKGLKGQTGPQGPKGETGPQGPPG 217          

HSP 17 Score: 54.299 bits (129), Expect = 9.020e-7
Identity = 50/118 (42.37%), Postives = 62/118 (52.54%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGP---------SGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGP------PGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA+G +G  G KG+ G          SG  G +G++G +G  G+ G QGP+G EG  G+KGD+G QG  G  G  G KGD G  GP       G  G  G  G +GP GN G  G K
Sbjct:  234 KGAKGAKGQQGPKGEMGEKGLKGLPGLSGTPGAKGDMGPQGPTGDLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGDVGPQGPGTKGEMGGTKGAVGAMGPQGPQGNTGAMGAK 351          

HSP 18 Score: 53.5286 bits (127), Expect = 1.916e-6
Identity = 45/109 (41.28%), Postives = 59/109 (54.13%), Query Frame = 2
Query: 1826 YKGARGIRGSSGAKGDHGPSGQVGT------RGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
             KG  G+ G+ GAKGD GP G  G       +G  G +G+KG+ G QG +G  G  G KGD+G QG        G KG  G++GP G  GN G  G++G +G  G  G+
Sbjct:  254 LKGLPGLSGTPGAKGDMGPQGPTGDLGPQGPKGHEGSQGLKGDMGQQGLKGAIGPLGPKGDVGPQGPGTKGEMGGTKGAVGAMGPQGPQGNTGAMGAKGEMGPNGLKGD 362          

HSP 19 Score: 52.373 bits (124), Expect = 3.577e-6
Identity = 42/102 (41.18%), Postives = 57/102 (55.88%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G +G  GS G KGD G   Q G +G +G  G KG+ G QGP     + G KG +G  G  GP+G  G  G +G +GP G  G+ G +G +G +G+ G  GE
Sbjct:  282 QGPKGHEGSQGLKGDMG---QQGLKGAIGPLGPKGDVGPQGPGTKGEMGGTKGAVGAMGPQGPQGNTGAMGAKGEMGPNGLKGDMGPQGLKGNMGSTGPKGE 380          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Medaka
Match: col1 (collagen type I alpha 1 [Source:NCBI gene;Acc:100144368])

HSP 1 Score: 113.62 bits (283), Expect = 1.306e-24
Identity = 218/555 (39.28%), Postives = 256/555 (46.13%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPG---PQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGD---------------------RGPPGIAGNMGQRGPNGPIGI---------VXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER---YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG+ GP+G  G PG  G  G +GPKG  G  G++G PG    +G+ G+ G  GP G+ G  G  G+ G  GP G  G RG PG  G PGP G  G  G                        + G RG++G     GP G  G+ GD GP G +G  G  GE G +G  G  G  G  GPQG  GEPG PG+ G+ G+ G  G                          G +GD G  G  GAPG +G AG  G  G++G +G  G  G  G  G  GP G QG+             G RG  G  G  G+ G QG +G  G  G  G +G  GPIGLPGP G  G  G+ GA GP G +G  G  G  G  G PG AG  GP G  G  G  GE G+                     +GP G  G  G RGP GP G          VGP GP G PGP GP G  G  GPKG+RG  G  G PG+ G        G KGSPG  GA GS G PGP G+ G +G  G PG +GE G  G  GP GE G+
Sbjct:  382 RGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAG------------------------KPGERGVMG---PTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGL------------------------TGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGM------------PGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENGAKGDAGPPGPAGPTGAPGPQGPVGNTGAKGARGPAGPPGATGFPGGAGRVGPPGPAGNPGPPGPTGQAGKEGPKGNRGETGPAGRPGEVGAAGPPGPSGEKGSPGADGAPGSAGLPGPQGIAGQRGIVGLPGQRGERGFPGLPGPAGEVGK 873          

HSP 2 Score: 94.3597 bits (233), Expect = 9.997e-19
Identity = 166/395 (42.03%), Postives = 195/395 (49.37%), Query Frame = 2
Query: 2921 GDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGER---------------GDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSR---GLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGP---IGPVGEDGE 4042
            G+VG  G  G  G +GE G  G++G  G  G  G +G  GEPG+PG  G        G++G+ G  G  G  G  G  G  GE+G  G +G  G TG PGR GE G PG  G +G+ G PG  G  G  G  G  G  G  G +G  G  G+ G  G  G  G  G  G +GV G  G  G PG+ G+ GP G  GP G +G  G+ G  G PG  G  G  G +G PG PG+ GL G AG  G  G  G+R               G RG PG AGN G +G  G  G  G  GP G  G  G  G  G PG +G R   G  G  G PG+ G R L G  G PG  GA G KGEPG  G  GP G RG PG +GE+G  GP    GP G DG+
Sbjct:  323 GEVGAPGAKGEPGAKGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGA------PGERGAPGGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQ 711          

HSP 3 Score: 91.6633 bits (226), Expect = 5.419e-18
Identity = 177/508 (34.84%), Postives = 209/508 (41.14%), Query Frame = 2
Query: 2507 GPSGLP---GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE---PGPPGLTGPQGKRGNPGPKGETGLMG 4012
            G  G P   G+PG  G+ G+ G +G  G  G+ G  G +G  GA G  G  GE G+ G RG  G  GP G KG                       G  G  G  G  G+ G  G  GE G+ G  G  GS G  GPDG+ G  G  G+ G  G  G VG +   G+ G+MG  GP+G +G+ G PG+ GV G  G  G  G  G +GP G  G  G  G RGE+G  G  G  G  G  G IGE G PG+ G  G  G PG+ G +GD G  G RG  G                             GP G  G  G  G  G KG+ G  G PG  G                            PGL G  G +G AG  G +G+ G++G +G  G  G  G RG  GPIG                              L G  G PG KGE    G  G  G  GA G +GE   PGP G  GP G  G PG KGE+G  G
Sbjct:  323 GEVGAPGAKGEPGAKGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKG---------------------APGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGAR---GQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPG---------------------------PVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGP---------------------------PGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIG------------------------------LPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENG 722          

HSP 4 Score: 82.8037 bits (203), Expect = 2.935e-15
Identity = 122/413 (29.54%), Postives = 174/413 (42.13%), Query Frame = 2
Query: 3833 LDGKVGEPGQKGERYLKGIKG---------SPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPV---GEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYK--RSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQ---QGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVD-HTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLE--TTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            L G  GE G+ GER +KG +G           G  G  G  G  GP G  GP G  G PG  G +GL GP GP    G  GE G AGP GP GP G PG  G   D G               I  P E +   ++  R+  A    D +  +   +  ++ +I++IR P G  K+  ARTC D+K+       G+Y+IDP++G  +D+I+V C   E  +TC+S  ++   Q   Y  K   + +  W      +G         S+  D + Q+ FLR  ++++ Q + + C  SV Y D+        K A +L   +   I      R                     F  ++  DGC   T G+  K  ++  T+  S LPIID    D         GL V  +CF
Sbjct:  965 LRGDKGETGEAGERGMKGHRGFTGMQGPPGPSGPPGEQGPAGSAGPAGPRGPAGSAGVPGKDGMSGLPGPTGPPGPRGRSGEMGPAGPPGPPGPAGAPGAPGGGFDLGF--------------IAQPQEKAPDPFRMFRADDANVLRDRDLEVDSTLKSLSQQIEQIRSPDGTRKNP-ARTCRDLKMCHPDWKSGEYWIDPDQGCTQDAIKVYCNM-ETGETCVSPTQREVAQKNWYISKNIKEKKHVWFGETMNDGFQFEYGSEGSLPEDVNIQLTFLRLMSTEASQNLTYHCKNSVAYMDAAA---GNLKKALLLQGSNEIEIRAEGNSR---------------------FTYSVLEDGCTSHT-GTWGKTVIDYKTSKTSRLPIIDIAPMDIGAP-DQEFGLEVGPVCF 1335          

HSP 5 Score: 82.0333 bits (201), Expect = 5.576e-15
Identity = 173/493 (35.09%), Postives = 202/493 (40.97%), Query Frame = 2
Query: 2597 GNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPI------GLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP---GPPGLTGP---QGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G PGP+G  G  G  G  GE G+KG+ G  G QGP G  G                                             E+G RG  G  G+ G  G  GE+G     G  G  G+ G  G KG+ G+ G  G  GP+G +GEPG  G+ G+                        PG  G  G  G  G  G  GP GAPG+ G  GPPG  GA G  G  G  G KG  G  G  GE+G+             G  G  G  G  G+SGPQG  G     GP G +GE GP       GLPGP G                             G PGK G QG  G +G  G  G RGDRG P      G+RG  GP+G  G  G  G  G  G  G  G PG  G++G  G  G PG++G   L G++G  G  GA G+ G P   G  GLTGP    G  G PG KGE G  GP+GP G  G
Sbjct:  314 GAPGPKGNNGEVGAPGAKGEPGAKGEAGAPGAQGPPGAHG---------------------------------------------EEGKRGARGEPGAPGARGAPGERGAP---GGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGL------------------------PGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGV------------MGPTGPAGAPGKDGDSGPQGPSGP---SGPAGERGEQGPAGAPGFQGLPGPQGAI---------------------------GEPGKPGEQGLPGDAGAPGLTGARGDRGFP------GERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARG 686          

HSP 6 Score: 71.2478 bits (173), Expect = 8.720e-12
Identity = 160/431 (37.12%), Postives = 190/431 (44.08%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPLG--------KTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGV--------KGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPR---GEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGE---------------LGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP---GPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G  GL G KG  G  G DG KGD GP G          G  G  G  G KG  G  G  G  GP G  GEPG+PG AG  G       +    P           +  P  +            G++G  G  G PG  GEAG PG  G  GA G+ G +G +G+ G  G R   GE+G  G  G  GS G  G +G PG +G+ G  GP+G  GE                G  G PG  G+ GP G PG  G  GP G  GA G PG  G  G  G  G  G PG+ G+ GP G +G  G+ G+ G +GP G +G  G+RG  GP G  G  G                       G  G +GEPG+ GE+ L G  G+PG  GA G +G P   G PG  GP G RG+PG  G  G  G  G  G  G
Sbjct:  199 GTPGLPGIKGHRGFSGLDGAKGDTGPAGPKVSGPTGPAGPPGFPGGPGAKGEIGPQGARGPEGPAGARGEPGNPGPAGPAGPSVSMKLECFGAPGAAGAPGFPGPRGPPGPQGAGGAPGPKGNNGEVGAPGAKGEPGAKGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLP---------------------GPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPG 608          

HSP 7 Score: 59.3066 bits (142), Expect = 3.934e-8
Identity = 42/96 (43.75%), Postives = 55/96 (57.29%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GS GA+G  G  G  G +G  G  G  GE G+ GP GP G  G  GD G QG +GP G  G +G++G  G PG+ G PG +G+   +G  G+ GE+
Sbjct:  458 GSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGA---IGEPGKPGEQ 550          

HSP 8 Score: 55.4546 bits (132), Expect = 6.104e-7
Identity = 42/103 (40.78%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +GA G+ G  G +GD G  G  G  G+ G +G+ G  G+ GP G  G  G  G  G  G  G RG  G +G+ G  GP G+AG PG  G  G  G  G +G K
Sbjct:  622 RGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENGAK 724          

HSP 9 Score: 54.299 bits (129), Expect = 1.315e-6
Identity = 44/109 (40.37%), Postives = 56/109 (51.38%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGE---------VGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            GA+G+ GS G+ G  G +G  G  G+         VG +G  G  G  GP+G  G AG  G+ G+ G TGP G  G  GD G  GP G +G  G +G +GP G  G  G
Sbjct:  425 GAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQG 533          

HSP 10 Score: 54.299 bits (129), Expect = 1.544e-6
Identity = 42/101 (41.58%), Postives = 52/101 (51.49%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KG  G  G+ G  G HG  G+ G RGE G  G +G  G +G  G  G  G  G  G +G  G RG  G  G +GS G PG  G PG+ G++G  G+ G  G
Sbjct:  337 KGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPG 437          

HSP 11 Score: 53.9138 bits (128), Expect = 1.782e-6
Identity = 47/106 (44.34%), Postives = 57/106 (53.77%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRG---KDGNKGDEGSVGPPGYAGNPGIK---GSRGPVGNQGRDG 2131
            G  G RGS G+ G+ G  G  G  G  G +G  G  G+ G RG  G+ G++GD G QG  G  G   KDG +G  G +G PG AG PG K   G+ GPVG  G  G
Sbjct:  581 GPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARG 686          

HSP 12 Score: 53.5286 bits (127), Expect = 2.219e-6
Identity = 45/102 (44.12%), Postives = 54/102 (52.94%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA G  G SG +G  GPSG  G RGE G  G  G  G+ GP+G  G  G  G+ G+ G+ G  G  G +GD G  G  G  G  G  G+RG  G+ G DG K
Sbjct:  497 GAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAK 598          

HSP 13 Score: 53.5286 bits (127), Expect = 2.604e-6
Identity = 41/95 (43.16%), Postives = 51/95 (53.68%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G+ GAKG+ G  G+ G  G  G  G  GE G +G RG  G  G +G  G +G  G RG  G+ G  G+ G PG  G PG+ G +G  G  GR GE
Sbjct:  326 GAPGAKGEPGAKGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGE 420          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Medaka
Match: col11a1a (collagen type XI alpha 1 chain [Source:NCBI gene;Acc:105356662])

HSP 1 Score: 112.079 bits (279), Expect = 3.890e-24
Identity = 144/496 (29.03%), Postives = 212/496 (42.74%), Query Frame = 2
Query: 3605 GLPGKAGLQGPAGGSGT---QGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTG------------------PQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDAL---FDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGD-SELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYM--DKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            G  G+AG +GPAG +G    QG AG+ G  G  GI G +G++G  G  G  GP GP G PG  G  G  G+ G KG  GL G +G PG++GE   KG +G PG  GA G KG+ G  G  G                   +G  G  GPKG+TGL+GP GP G  GE                                       + IR+P +        S  A + +D  + +   F  ++ +   I+R+++P G  +++ ARTC D++L   +  D +Y+IDPN+G   DS +V C F+   +TCI    +  G+     P +   +W +  +  G      D     ++  QM FL+   + + Q   + C QSV + D S  S E   ++                   LG N+  M  D   Y          +T   DGC  R     +  +L T  +  +PI+D   ND+ +      G +V  +CF
Sbjct: 1347 GAKGEAGAEGPAGKTGPVGPQGPAGKPGPEGLRGIPGPVGEQGLPGAPGQDGPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGE---KGDRGLPGPQGAPGGKGDSGMSGTAGPLGPPGPPGLPGPQGPKGAKGSSGPAGPKGDTGLIGPPGPPGPPGE-----------------------------------VIEPLPIRSPKKSKRSADMPSDTAGTIMDYGEGMEDIFGSLNSLKQDIERMKYPMG-TQNNPARTCKDLQLCHPEFPD-EYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDNKSSGVRISSWPKEVPGSWFSEFK-HGKTLNYVDADGNSINMVQMTFLKLLTASARQNFTYHCHQSVAWHDASSDSHERALRF-------------------LGANDEEMSFDNNPY----------ITAIADGCETRNGYGKTVLELNTPKIDQVPIVDVMFNDFGDPN-QKFGFDVGPVCF 1771          

HSP 2 Score: 107.842 bits (268), Expect = 7.331e-23
Identity = 66/228 (28.95%), Postives = 106/228 (46.49%), Query Frame = 2
Query:  245 SYRDGVVDLLTAVQISDKNIGTTAEEPFCGNSRRK----AFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRD----PSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYV----LQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGPV 892
            + R   VD+L  +   +   G T    FC N R      A+R      + A +  +F G FP++FSIL T + ++     + SI N+     L V  GR     Y D    P+   +  L    +L DG+WHR+A SV+K +V++++DC+   +  L R SD   +S   + V    + D  GD      +  LL+V     A+  C+ Y+P+C+    D +  Q P+
Sbjct:   34 AVRAEPVDVLKVLAFQNYPEGVTRTSGFCENRRASRPDSAYRVAKNIQISAPTTQVFPGVFPEDFSILTTLRPKSGLQSFLLSIYNEQGVQQLGVEVGRSPVFLYEDQNGKPAPEDY-PLFRALNLADGRWHRVAISVEKKTVTIIVDCKKTITKPLPR-SDHASVSTDGITVFGTRILDEEGD------IQQLLIVDDPKAAYDYCEHYSPECDTPQRDSLQAQEPL 253          

HSP 3 Score: 64.6994 bits (156), Expect = 1.002e-9
Identity = 52/105 (49.52%), Postives = 64/105 (60.95%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGD-HGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNK-GDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            KGA G+RG  G+KG+  G  G  G +G++G+KG +GE G+ GPRG +G  G KG  G  GE+GP G  G K G  G  G PGY G  G KGS G  G  G +GEK
Sbjct:  764 KGADGVRGLKGSKGEKQGEDGFPGFKGDMGIKGDRGEIGLLGPRGEDGPEGPKGRAGPTGESGPLGPAGEKQGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEK 868          

HSP 4 Score: 63.1586 bits (152), Expect = 3.456e-9
Identity = 134/367 (36.51%), Postives = 159/367 (43.32%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKF---------------GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGP-QGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASG-------AEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGT 3520
            G PG++G  G  G PG+ G++G    +G+ GP G +G PG  G RG  G+ GP G  G  G RG                 G  GP G KG  G  G  GPPG  G  G       P    G       GPQG  G  G+ G  G  G  G  GP GEKG  GPLG  G  G  G  G+KG++G  GL GS+G  QG  G PG  GD G+                                 KG +G+IGL+GP G  G  G  G  G TG SG        +G+ GV G  G  G  GP+G  G  G  G  G KG +G             +G QG  G+ G  GP G +G  GP G PGP GT
Sbjct:  588 GEPGSKGDRGFDGLPGLPGEKGH---RGEPGPMGPTGAPGEDGQRGEDGEIGPRGLAGETGPRGLLGPRGPPGPPGGPGVAGVDGPPGPKGNMGPQGEPGPPGQQGIPGTQQGLPGPQGPIGPPGEKLQGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGAQGPLGPQGPIGYPGPRGVKGADGVRGLKGSKGEKQGEDGFPGFKGDMGI---------------------------------KGDRGEIGLLGPRGEDGPEGPKGRAGPTGESGPLGPAGEKQGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGI------------AGKQGPRGQRGPTGPRGARGARGPTGKPGPKGT 906          

HSP 5 Score: 60.4622 bits (145), Expect = 1.801e-8
Identity = 152/429 (35.43%), Postives = 177/429 (41.26%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKP---------GIDGKEGSIGPKGDLGPRGQSGNPGP-QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIG 3742
            RGPPG  G +G  G           G  G  G  GP+G  GP GQ+G  G  QG  GA G  G  GE GSKGDRG  G  G  G+KG R                                                   G  G +GP G  GE G  G  G+ G +G  GE G +G  G  G  G  G  GV+G  G PG  G  G +GE G  G QG  G       P  P     + ++G  G  G  G PG  G  G PGK G  G +G  G  G +G  G  GPRG +G D              +G+ G  G  G+ G +G  GE+G+ GP G  G  GP G  GP G SGP G  G      G            PGLPG  G QGP G +G  G  G  G++G  GIAG  G RG  GP G
Sbjct:  523 RGPPGPMGLTGRSGPVGPPGPAGLKGEGGDPGPQGPRGIQGPPGQAGKSGKRQGRSGADGARGMPGEPGSKGDRGFDGLPGLPGEKGHR---------------------------------------------------GEPGPMGPTGAPGEDGQRGEDGEIGPRGLAGETGPRGLLGPRGPPGPPGGPGVAGVDGPPGPKGNMGPQGEPGPPGQQGIPGTQQGLPGPQGPIGPPGEKLQGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGAQGPLGPQGPIGYPGPRGVKGAD--GVRGLKGSKGEKQGEDGFPGFKGDMGIKGDRGEIGLLGPRGEDGPEGPKGRAGPTGESGPLGPAGEKQGKLGV-----------PGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKQGPRGQRGPTG 887          

HSP 6 Score: 60.4622 bits (145), Expect = 2.131e-8
Identity = 150/435 (34.48%), Postives = 184/435 (42.30%), Query Frame = 2
Query: 2855 KGVRGLLGRKGSVGPIGP------DGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQ---------------------PGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGI------------QGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXS-PGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            +G  G +G  G  GP+GP       GE GD GP G  G++G  G+ G  G   + G  G+ G +G+ GEPGS GD G +                        G PG  GEKG +G+ G +GPTGAPG  G+ G  G+ G  G  G+                      GV G  G  G +GP+GE G  G  G  G++    G   P         GPQG  G  G+            +GPPG KG  GP+G  GP+G  GP G  GA G  G  G  G+       PG  G  G++G  G  G  G  GE G  GP G AG  G+ GP GP G                            +G  G  G PG  G +  KG  G PG  GA G KG  G  G  GP+G+RG  GP+G  G  GP G  G  G
Sbjct:  523 RGPPGPMGLTGRSGPVGPPGPAGLKGEGGDPGPQGPRGIQGPPGQAGKSGK--RQGRSGADGARGMPGEPGSKGDRGFD------------------------GLPGLPGEKGHRGEPGPMGPTGAPGEDGQRGEDGEIGPRGLAGETGPRGLLGPRGPPGPPGGPGVAGVDGPPGPKGNMGPQGEPGPPGQQGIPGTQQGLPGPQGPIGPPGEKLQGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGAQGPLGPQGPIGYPGPRGVKGADGVRGLKGSKGEKQGEDGFPGFKGDMGIKGDRGEIGLLGPRGEDGPEGPKGRAGPTGESGPLGPAG--------------------------EKQGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKQGPRGQRGPTGPRGARGARGPTGKPGPKG 905          

HSP 7 Score: 52.373 bits (124), Expect = 5.622e-6
Identity = 43/98 (43.88%), Postives = 55/98 (56.12%), Query Frame = 2
Query: 1844 IRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGD-EGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            ++G  G  G  G  G  G  G  G +G  GE G QGP GP+G  G  G  G++G  G RG  G+KG+ +G  G PG+ G+ GIKG RG +G  G  GE
Sbjct:  712 LQGPQGRSGLPGLPGADGPPGHPGKEGPPGEKGAQGPLGPQGPIGYPGPRGVKGADGVRGLKGSKGEKQGEDGFPGFKGDMGIKGDRGEIGLLGPRGE 809          

HSP 8 Score: 51.9878 bits (123), Expect = 7.057e-6
Identity = 76/213 (35.68%), Postives = 92/213 (43.19%), Query Frame = 2
Query: 3425 GPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXX---XXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERY----LKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            GPQG  G+ G +GPPGR G  G  GLPGP GT               P  S Q      + S   P +  ++GP G  G  G +G  G  GP G+ G  G  GP GP                              RG+ G  G+ G+ G+R       G +G PG  G+ G +G  G PGL G +G RG PGP G TG  G  G  GEDGE
Sbjct:  453 GPQGTPGDPGDRGPPGRAGLPGADGLPGPPGTMLMLPFRFGGDGEKGPVVSAQEAQAQAILSQARP-QLAMRGPPGPMGLTGRSGPVGPPGPAGLKGEGGDPGPQGP------------------------------RGIQGPPGQAGKSGKRQGRSGADGARGMPGEPGSKGDRGFDGLPGLPGEKGHRGEPGPMGPTGAPGEDGQRGEDGE 634          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Medaka
Match: col1 (collagen type I alpha 1 [Source:NCBI gene;Acc:100144368])

HSP 1 Score: 111.309 bits (277), Expect = 5.652e-24
Identity = 218/555 (39.28%), Postives = 256/555 (46.13%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPG---PQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGD---------------------RGPPGIAGNMGQRGPNGPIGI---------VXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQ---KGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            RG PG+ GP+G  G PG  G  G +GPKG  G  G++G PG    +G+ G+ G  GP G+ G  G  G+ G  GP G  G RG PG  G PGP G  G  G                        + G RG++G     GP G  G+ GD GP G +G  G  GE G +G  G  G  G  GPQG  GEPG PG+ G+ G+ G  G                          G +GD G  G  GAPG +G AG  G  G++G +G  G  G  G  G  GP G QG+             G RG  G  G  G+ G QG +G  G  G  G +G  GPIGLPGP G  G  G+ GA GP G +G  G  G  G  G PG AG  GP G  G  G  GE G+                     +GP G  G  G RGP GP G          VGP GP G PGP GP G  G  GPKG+RG  G  G PG+    G     G KGSPG  GA GS G PGP G+ G +G  G PG +GE G  G  GP GE G+
Sbjct:  476 RGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAG------------------------KPGERGVMG---PTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGL------------------------TGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGM------------PGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENGAKGDAGPPGPAGPTGAPGPQGPVGNTGAKGARGPAGPPGATGFPGGAGRVGPPGPAGNPGPPGPTGQAGKEGPKGNRGETGPAGRPGEVGAAGPPGPSGEKGSPGADGAPGSAGLPGPQGIAGQRGIVGLPGQRGERGFPGLPGPAGEVGK 967          

HSP 2 Score: 102.064 bits (253), Expect = 4.262e-21
Identity = 72/278 (25.90%), Postives = 116/278 (41.73%), Query Frame = 2
Query: 4196 RYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQ---QGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVD-HTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSK--FKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            R  R+  A    D +  +   +  ++ +I++IR P G  K+ A RTC D+K+       G+Y+IDP++G  +D+I+V C   E  +TC+S  ++   Q   Y  K   + +  W      +G         S+  D + Q+ FLR  ++++ Q + + C  SV Y D+        K A +L   +   I      R                     F  ++  DGC   T G+  K     +T+  S LPIID    D         GL V  +CF
Sbjct: 1196 RMFRADDANVLRDRDLEVDSTLKSLSQQIEQIRSPDGTRKNPA-RTCRDLKMCHPDWKSGEYWIDPDQGCTQDAIKVYCNM-ETGETCVSPTQREVAQKNWYISKNIKEKKHVWFGETMNDGFQFEYGSEGSLPEDVNIQLTFLRLMSTEASQNLTYHCKNSVAYMDAAA---GNLKKALLLQGSNEIEIRAEGNSR---------------------FTYSVLEDGCTSHT-GTWGKTVIDYKTSKTSRLPIIDIAPMDIGAP-DQEFGLEVGPVCF 1445          

HSP 3 Score: 100.138 bits (248), Expect = 1.488e-20
Identity = 166/406 (40.89%), Postives = 200/406 (49.26%), Query Frame = 2
Query: 2852 EKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIG------LPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGP---IGPVGEDGE 4042
            E G +G  G  G+ GP G  GE+G  G  G+ G  G RG  G +G+ G  G  GS GP G  G PG  G  G+                  VG +G  G+PG  GE G+ G  G+ G  G+PG  G+ GP G  G  G  G PG  G +G  G++G  G +G  G  G  G +G+ G  G  G  G  G+SGPQG  G     GP G +GE GP G      LPGP G  G  GK G  G PG +G  G  G  G  G PG+ G  GP G +G +G  G  G+ G  G AG  G  G  GP G+      +G PG  G  G  G  G +G +G  G  G PG+ G R L G  G PG  GA G KGEPG  G  GP G RG PG +GE+G  GP    GP G DG+
Sbjct:  427 EPGAKGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKGAPGERGAPGL------------------VGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGP---SGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGL------QGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQ 805          

HSP 4 Score: 89.7373 bits (221), Expect = 2.234e-17
Identity = 177/508 (34.84%), Postives = 209/508 (41.14%), Query Frame = 2
Query: 2507 GPSGLP---GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGE---PGPPGLTGPQGKRGNPGPKGETGLMG 4012
            G  G P   G+PG  G+ G+ G +G  G  G+ G  G +G  GA G  G  GE G+ G RG  G  GP G KG                       G  G  G  G  G+ G  G  GE G+ G  G  GS G  GPDG+ G  G  G+ G  G  G VG +   G+ G+MG  GP+G +G+ G PG+ GV G  G  G  G  G +GP G  G  G  G RGE+G  G  G  G  G  G IGE G PG+ G  G  G PG+ G +GD G  G RG  G                             GP G  G  G  G  G KG+ G  G PG  G                            PGL G  G +G AG  G +G+ G++G +G  G  G  G RG  GPIG                              L G  G PG KGE    G  G  G  GA G +GE   PGP G  GP G  G PG KGE+G  G
Sbjct:  417 GEVGAPGAKGEPGAKGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKG---------------------APGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGAR---GQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPGP---------------------------VGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGP---------------------------PGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIG------------------------------LPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENG 816          

HSP 5 Score: 88.1965 bits (217), Expect = 7.410e-17
Identity = 210/507 (41.42%), Postives = 251/507 (49.51%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLG------PRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGA---PGRIGEAGPPGKTGASG---AEGQPGVKGTKGDTGI---------------------------------LGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER 3874
            GP G  GP+G PG+ G  G  GS+G +G  G      P+G +G+ G  G RG  G +GP G  G  GD G  GP GP+G  GERG+ G  G PG  G  GP G  G PG  G +G+ G+ G  G  G +G RG  G +G+ GP+GP G +G  G  G  G KG  G  G  G++G  GL G  G +G +G PG  GD G  G KG  G  G  G  G  G  G+PG  G  G+KG  G  G +GP GA   PG  GE+GPPG  G +G   ++GQPG KG  G+ G                                   GP G  G  G  G +G  G  G  G PG  G  G  GP+G  GE G   P GR GE+G  G PGP G  G  G DGAPG               S GLPG  G+ G  G  G  G+ GERG  G PG AG +G++GP GP G  GP GP G PG  GP G  G  G  G+ G  G+ G PG KG+R
Sbjct:  531 GPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENGAKGDAGPPGPAGPTGAPGPQGPVGNTGAKGARGPAGPPGATGFPGGAGRVGPPGPAGNPGPPGPTGQAGKEGPKGNRGETG---PAGRPGEVGAAGPPGPSGEKGSPGADGAPG---------------SAGLPGPQGIAGQRGIVGLPGQRGERGFPGLPGPAGEVGKQGPVGPGGERGPPGPSGPPGLAGPPGEPGREGTPGNEGSAGRDGAPGPKGDR 1019          

HSP 6 Score: 80.4925 bits (197), Expect = 1.590e-14
Identity = 173/493 (35.09%), Postives = 202/493 (40.97%), Query Frame = 2
Query: 2597 GNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPI------GLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEP---GPPGLTGP---QGKRGNPGPKGETGLMGPIGPVGEDG 4039
            G PGP+G  G  G  G  GE G+KG+ G  G QGP G  G                                             E+G RG  G  G+ G  G  GE+G     G  G  G+ G  G KG+ G+ G  G  GP+G +GEPG  G+ G+                        PG  G  G  G  G  G  GP GAPG+ G  GPPG  GA G  G  G  G KG  G  G  GE+G+             G  G  G  G  G+SGPQG  G     GP G +GE GP       GLPGP G                             G PGK G QG  G +G  G  G RGDRG P      G+RG  GP+G  G  G  G  G  G  G  G PG  G++G  G  G PG++G   L G++G  G  GA G+ G P   G  GLTGP    G  G PG KGE G  GP+GP G  G
Sbjct:  408 GAPGPKGNNGEVGAPGAKGEPGAKGEAGAPGAQGPPGAHG---------------------------------------------EEGKRGARGEPGAPGARGAPGERGAP---GGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGL------------------------PGAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGV------------MGPTGPAGAPGKDGDSGPQGPSGP---SGPAGERGEQGPAGAPGFQGLPGPQGAI---------------------------GEPGKPGEQGLPGDAGAPGLTGARGDRGFP------GERGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARG 780          

HSP 7 Score: 63.5438 bits (153), Expect = 2.274e-9
Identity = 88/266 (33.08%), Postives = 108/266 (40.60%), Query Frame = 2
Query: 3245 GKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G+ GA GA+G+PG KG  G  G  GP G  G +G                          G +G  G  G +G PG +G  G  G PG  G +G  G                     +PG  G  GL GP G +G  G  GE G  G  G+ G+ G  GP+G  G  G                        + G DG+ G PG  G R   G+ G PG  GA G  G+PG  G+ GP G  G PG  G++G  GP GP G  GE
Sbjct:  417 GEVGAPGAKGEPGAKGEAGAPGAQGPPGAHGEEG------------------------KRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKG---------------------APGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPGPDGKTGPAG------------------------APGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGE 613          

HSP 8 Score: 58.9214 bits (141), Expect = 4.979e-8
Identity = 201/571 (35.20%), Postives = 233/571 (40.81%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIK------------------GSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRG---------------------PPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKG---------SPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG---------------ETGLM 4009
            RG PG  GP+G  G PG  G +G+ G  G  G  G  G PG QG+ G RG +G  G  G +GD+G  G  G  G  G RG  G  G PGPAG  G  G  G+ G  G                 G RG  G +G  GP GP G  G  G  G+ G KG  GE G K                  G  G TG  G+RGP G  G  G PG AG              GP GP G  G PG  G  G++G KG+ G  GP G PG +G AGPPG    SG +G PG  G  G  G+                                     GPQG+ G+ GI G PG++GE G  GLPGP G  G  G  G  G  G  G +G  G  G PG PG+ G  G  G +G  G  G +GDRG                     P G +G+ G+ GP GP G                 GP G  GP G  GL G  GE G+ GER +KG +G           G  G  G  G  GP G  GP G  G PG  G                +G M
Sbjct:  668 RGAPGPVGPAGARGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGMPGERGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVG---------------PAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENGAKGDAGPPGPAGPTGAPGPQGPVGNTGAKGARGPAGPPGATGFPGGAG------------RVGPPGPAGNPGPPGPTGQAGKEGPKGNRGETGPAGRPGEVGAAGPPGP---SGEKGSPGADGAPGSAGL------------------------------------PGPQGIAGQRGIVGLPGQRGERGFPGLPGPAGEVGKQGPVGPGGERGPPGPSGPPGLAGPPGEPGREGTPGNEGSAGRDGAPGPKGDRGESGPSGAPGAPGPPGAPGPVGPAGKSGDRGETGPAGPAGA---------------SGPAGPRGPAGPPGLRGDKGETGEAGERGMKGHRGFTGMQGPPGPSGPPGEQGPAGSAGPAGPRGPAGSAGVPGKDGMSGLPGPTGPPGPRGRSGEM 1157          

HSP 9 Score: 58.9214 bits (141), Expect = 6.148e-8
Identity = 43/99 (43.43%), Postives = 56/99 (56.57%), Query Frame = 2
Query: 1841 GIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  GS GA+G  G  G  G +G  G  G  GE G+ GP GP G  G  GD G QG +GP G  G +G++G  G PG+ G PG +G+   +G  G+ GE+
Sbjct:  549 GPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGA---IGEPGKPGEQ 644          

HSP 10 Score: 54.6842 bits (130), Expect = 1.063e-6
Identity = 42/103 (40.78%), Postives = 53/103 (51.46%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            +GA G+ G  G +GD G  G  G  G+ G +G+ G  G+ GP G  G  G  G  G  G  G RG  G +G+ G  GP G+AG PG  G  G  G  G +G K
Sbjct:  716 RGAAGLPGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGERGESGPPGPAGFAGPPGSDGQPGAKGESGENGAK 818          

HSP 11 Score: 53.5286 bits (127), Expect = 2.176e-6
Identity = 44/109 (40.37%), Postives = 56/109 (51.38%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGE---------VGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            GA+G+ GS G+ G  G +G  G  G+         VG +G  G  G  GP+G  G AG  G+ G+ G TGP G  G  GD G  GP G +G  G +G +GP G  G  G
Sbjct:  519 GAKGMTGSPGSPGPDGKTGPAGAPGQDGRPGPPGSVGARGQPGVMGFPGPKGAAGDAGKPGERGVMGPTGPAGAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQG 627          

HSP 12 Score: 53.5286 bits (127), Expect = 2.408e-6
Identity = 42/101 (41.58%), Postives = 52/101 (51.49%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            KG  G  G+ G  G HG  G+ G RGE G  G +G  G +G  G  G  G  G  G +G  G RG  G  G +GS G PG  G PG+ G++G  G+ G  G
Sbjct:  431 KGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGEPGLPGAKGMTGSPGSPG 531          

HSP 13 Score: 53.1434 bits (126), Expect = 2.948e-6
Identity = 46/109 (42.20%), Postives = 60/109 (55.05%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSR------GPVGNQGRDGEK 2137
            +G+ G  G+ GAKGD G  G  G +G  G++G+ GE G  G     G+ G +GD G +G  G  GKDG +G  G +G PG AG PG KG        GP G +G  GE+
Sbjct:  680 RGSPGSAGNDGAKGDAGAPGAPGAQGPPGLQGMPGERGAAGL---PGLRGDRGDQGAKGADGAPGKDGARGLTGPIGLPGPAGAPGDKGEPGAAGPVGPAGARGAPGER 785          

HSP 14 Score: 52.7582 bits (125), Expect = 3.764e-6
Identity = 45/102 (44.12%), Postives = 54/102 (52.94%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GA G  G SG +G  GPSG  G RGE G  G  G  G+ GP+G  G  G  G+ G+ G+ G  G  G +GD G  G  G  G  G  G+RG  G+ G DG K
Sbjct:  591 GAPGKDGDSGPQGPSGPSGPAGERGEQGPAGAPGFQGLPGPQGAIGEPGKPGEQGLPGDAGAPGLTGARGDRGFPGERGAPGPVGPAGARGSPGSAGNDGAK 692          

HSP 15 Score: 52.7582 bits (125), Expect = 3.796e-6
Identity = 41/95 (43.16%), Postives = 51/95 (53.68%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G+ GAKG+ G  G+ G  G  G  G  GE G +G RG  G  G +G  G +G  G RG  G+ G  G+ G PG  G PG+ G +G  G  GR GE
Sbjct:  420 GAPGAKGEPGAKGEAGAPGAQGPPGAHGEEGKRGARGEPGAPGARGAPGERGAPGGRGFPGSDGPAGAKGAPGERGAPGLVGPKGSTGEPGRTGE 514          
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Medaka
Match: col5a1 (collagen alpha-1(V) chain [Source:NCBI gene;Acc:101155530])

HSP 1 Score: 107.842 bits (268), Expect = 7.022e-23
Identity = 61/264 (23.11%), Postives = 114/264 (43.18%), Query Frame = 2
Query: 4220 QSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            + + D  + +F  ++ +  +I++++ P G  + + ARTC D++L   + TD +Y+IDPN+G  +DS +V C F+   ++CI   K+  G    K P      W +  +    +S   D     +   QM FLR  ++ + Q + + C QSV + D +   ++ +  A   +                   G  D+ +  D    P     +  DGC  +     +  ++ T  +  +P +D   ND+        G  V  +CF
Sbjct: 1266 KDYDDGMEEIFGSLNSLKQEIEQMKHPLG-TQSNPARTCKDLQLCHPEFTD-EYWIDPNQGCSRDSFKVYCNFTAGGESCIFPDKKSEGSKMAKWPKDSPGTWYSRYKRGSMLSYV-DAEGNPIGVVQMTFLRLLSAAARQNMTYNCYQSVAWHDQQ---QDSYDKAIRFL-------------------GSNDEEMSYDN--NPYIRAVV--DGCALKKGYEKTVLEINTPKVEQVPFVDIMFNDFGGS-TQKFGFEVGPVCF 1499          

HSP 2 Score: 68.5514 bits (166), Expect = 6.528e-11
Identity = 159/393 (40.46%), Postives = 190/393 (48.35%), Query Frame = 2
Query: 2870 LLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLP------GPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSK---GEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            L G  G  GP GP G  G  GP GK G +G  G  G +G  G+TG  G RG  G++G PG  G  G                       G  G PG  GE G +GD G IGP G P   GE GP G  G  G +G PG  G  G  G  GP+G                 G +G+PG  G  GN G QG+ G  G  GPPG KG  G  GLP      GP G  G  G  G  G  G  G  G  G  G  G+ G  G++G  G  G +GE G  G +G  GI G+ G+ GP GP G  GP+GP+G+ G  G  GPIG  G KG  G+ G  G PG++G +  +G +G PG  G  G++   G+PGP G  GP G RG  GP+G TG  GP G
Sbjct:  239 LKGESGEGGPQGPRGPMGSAGPPGKPGRRGRSGADGARGMPGQTGPKGDRGFDGLAGLPGEKGHRG---------------------EAGPQGPPGSPGEDGERGDDGEIGPRGLP---GEPGPRGLLGPKGPQGPPGPPGVTGMDGQPGPKGN---------------IGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPTGEKGHMGPPGPQGPIGYPGPRGVKGADGVRGLKGNKGEKGEDGFPGFKGDMGIKGDRGELGPAGPRGEDGPEGPKGRSGLPGDPGPIGPGGEKGKLGVPGLPGYPGRQGPKGSQGFQGFPGANGEKGTRGTGGKPGPRGQRGPTGPRGERGPRGPTGKAGPKG 592          

HSP 3 Score: 55.0694 bits (131), Expect = 9.506e-7
Identity = 78/219 (35.62%), Postives = 99/219 (45.21%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQS------------------GNPGPQGIRGARGQSGPTGE---------LGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDA 3064
            GPPG +GP+G PG PG+ G +G  G  G  GP G+                   G  G  G+RG +G  G  GE         +G KGDRG+ GP GP G+ G                       G  G  G  G  G  GP G  G+ GV GL G  G  GP G  G +G  G  G+ G +GT G+ G +G  G TG  G RGP+G +G+ G  G++
Sbjct:  397 GPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPTGEKGHMGPPGPQGPIGYPGPRGVKGADGVRGLKGNKGEKGEDGFPGFKGDMGIKGDRGELGPAGPRGEDGPE---------------------GPKGRSGLPGDPGPIGPGGEKGKLGVPGLPGYPGRQGPKGSQGFQGFPGANGEKGTRGTGGKPGPRGQRGPTGPRGERGPRGPTGKAGPKGNS 594          
BLAST of Collagen type IV alpha 2 chain vs. Planmine SMEST
Match: SMESG000077782.1 (SMESG000077782.1)

HSP 1 Score: 1183.32 bits (3060), Expect = 0.000e+0
Identity = 840/843 (99.64%), Postives = 842/843 (99.88%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCFS 5014
            RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERGDPGSQGPPGPAGPNGPIGVAGSPGDKGGKGVVGNPGPQGPIGEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQGIPGQPGPRGEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDGP+GPIGSKGLKGGRGKPGRQGSTGNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTSGPSGKDGAPGPPGGSGQAGDDGPVGSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVGPDGPRGQPGPIGPMGPIGNPGPKGSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGESGAAGPLGPYGPPGPPGDRGLPGDSGDRGGQGRPGPPGQVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVF+EEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRA DLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCFS
Sbjct:  781 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERGDPGSQGPPGPAGPNGPIGVAGSPGDKGGKGVVGNPGPQGPIGEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQGIPGQPGPRGEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDGPIGPIGSKGLKGGRGKPGRQGSTGNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTSGPSGKDGAPGPPGGSGQAGDDGPVGSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVGPDGPRGQPGPIGPMGPIGNPGPKGSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGESGAAGPLGPYGPPGPPGDRGLPGDSGDRGGQGRPGPPGQVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFNEEVKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRASDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCFS 1623          

HSP 2 Score: 686.411 bits (1770), Expect = 0.000e+0
Identity = 439/439 (100.00%), Postives = 439/439 (100.00%), Query Frame = 2
Query:  146 MLNITSLKRGVXXXXXXXXXXXXXXXXXXTQALSYRDGVVDLLTAVQISDKNIGTTAEEPFCGNSRRKAFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGPVKXXXXXXXXXXXXXXXXXXXXXXXXXNGRDGLPGTPGVPGAPGHIFVLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIXXXXXXXXXXXXXXXXXXXTDGERGSNGLTGPKGRSGDIGMQGLPGNKXXXXXXXXXXXXXXXXXXXXXXASGRPGDV 1462
            MLNITSLKRGVLISRISLVSILLILYLSSTQALSYRDGVVDLLTAVQISDKNIGTTAEEPFCGNSRRKAFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGPVKKEETGGQQGPPGPKGPKGPKGQEGKNGRDGLPGTPGVPGAPGHIFVLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIGPRGLRGMRGIPGPPGRSGTDGERGSNGLTGPKGRSGDIGMQGLPGNKGEKGYRGGRGSKGSLGRRGELGASGRPGDV
Sbjct:    1 MLNITSLKRGVLISRISLVSILLILYLSSTQALSYRDGVVDLLTAVQISDKNIGTTAEEPFCGNSRRKAFRYESGNSLMAKSDVLFQGKFPKEFSILITAKSEALDSQDVFSIRNKNNNIILAVTYGRQITLRYRDPSSTSFMNLPFEFDLTDGQWHRIAFSVDKSSVSLLIDCQFIGSMKLNRTSDPLDISGGFLYVLQDHNGDASFTGILDSLLLVGGASEAFKQCQIYNPDCNNDFNDVMMNQGPVKKEETGGQQGPPGPKGPKGPKGQEGKNGRDGLPGTPGVPGAPGHIFVLPIPDSEENSNRRVAQFRTSIKRTASLLKGNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIGPRGLRGMRGIPGPPGRSGTDGERGSNGLTGPKGRSGDIGMQGLPGNKGEKGYRGGRGSKGSLGRRGELGASGRPGDV 439          

HSP 3 Score: 204.142 bits (518), Expect = 2.378e-52
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 2
Query: 1709 VQGSAGLPGNPXXXXXXXXXXXXXXXXXXXXXXXXXXXXYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            VQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK
Sbjct:  522 VQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 664          

HSP 4 Score: 98.2117 bits (243), Expect = 4.557e-20
Identity = 217/604 (35.93%), Postives = 264/604 (43.71%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEG---------------SIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEK---GVKGDIGLIGPTGAPG---------------RIGEAGPPGK---TGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGR---KGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSP------------------------GLPGKAGLQGPAGGSGTQGEAGE------RGDRGPPGIAGN------MGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNP------------GPKGETGLMGPIGPVGEDG 4039
            GP G +G +GLPG PG  G  G               + GP G  G RG  G+ G +G  G  GQ G  GE+G KG +G++G QGP G +G                                               GV+G +G +G  GP G DG KGD G +G  G  G  G  G +G  G  G  G +G  G++G PG  G  G  G  G +G  G  G  G  G QG  G  G  G     G +G IG IG +G PG                 GE G PG+    G  G+ GQPG +G+ G+ G  G RG  GL G  G  G +G  G +G  G +G +GN GPQG+ G  G  GP G    KG+ G  G  GP G  G  G  G+ GPPG +G  G  G  GSP                        GL G+ G  GP G  G +G+ G       +G RG  GI G+      MG RGP G  G  G  G  G  G  G  G  G+ GP G  G+ G  G+PG +GE+ +KG  G  G  GA G  GE GPPG TG  G  G P            GP+GE GL GPIGP+G  G
Sbjct:  518 GPQGVQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG---------------------------------------------IAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEKGDPGIAGTPGGNGPVGPIGSPGPRGGPGPSGTLGSKGEQGPGGPTGMPGPVGPVGPRGTIGFIGVSGPPGTSGRPGPPGYPGERGETGEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERGDPGSQGPPGPAGPNGPIGVAGSPGDKGGKGVVGNPGPQGPIGEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQGIPGQPGPRGEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDGPIGPIGSKG 1076          

HSP 5 Score: 97.8265 bits (242), Expect = 5.723e-20
Identity = 191/560 (34.11%), Postives = 248/560 (44.29%), Query Frame = 2
Query: 2543 GKEGSIGPKGDLGPRGQS------------------GNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX------------EKGVKGDIGL---IGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLK---------GIKGSPGTIGAVGSKGEPGPPGLTGPQGKR------------------GNPGPKGETGLMGPIGPVGEDGE 4042
            G  G+IG  G  GP G S                  G PG  G  G +G  G  GE GS G+RG  G +GP+G  G+                                                 G+ G++GS+GP      KGD+GP G++G  G +G  G +G  G TG +GS+G +G  G  G  GD G  G+ G QG  G  GP GP+GV G PG  G +G            EKGV+G +G    +GP G  G  G+ GP GKTG  G  G+ G+KG++G TG++G RG QG+ G  G  G  G+ G +G+ G+QGS G +GP GV+G  G  GP G KG  G IGL GP G                           +PG  G+AG  G  G SG +G+ G +G +G  GI G  G++G +GPIG +                        GS+GL G  G+PG++G              GI+G PG  G +G  G PGP G +GP GK                   G+PG  G+ GL GP G  G  GE
Sbjct:  719 GPRGTIGFIGVSGPPGTSGRPGPPGYPGERGETGEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRGPSGLPGK------------------------------------------------PGIDGKEGSIGP------KGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERGDPGSQGPPGPAGPNGPIGVAGSPGDKGGKGVVGNPGPQGPIGEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQGIPGQPGPRGEKGVKGDIGLIGPTG---------------------------APGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDGPIGPI------------------------GSKGLKGGRGKPGRQGSTGNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTSGPSGKDGAPGPPGGSGQAGDDGPVGSPGLPGKAGLQGPAGGSGTQGE 1173          

HSP 6 Score: 92.0485 bits (227), Expect = 3.665e-18
Identity = 214/545 (39.27%), Postives = 262/545 (48.07%), Query Frame = 2
Query: 2498 GTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTG---------------DKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRG---PQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSG------------TQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            G RGP G+ G PG  G  G  G KGD G  G +G  GP+G+RG RG  GP G  G+ G+RG  G  GP G               +KG RG  GS+G  G  G  G  G  G  G  G  G  G  GP+GP G +G+RGL G  G  GP G  GE G++G  G  G  G +G +G +G  G +G  G RG   PQGV G  G PG+ G  G+ GE+G  G  GP G +G  G           G KG  G+ G +GA G   + GP G+ G     G+ G+KG KG+ GI GPRG +G+                               GV+G++GIQG  G +G+ G  G  G VG  G +G  G  G  G  G  G DG  G PG+ G  G  GP G  G            T G  GE+G  GP G+ G +G  GP G IG +G  GP G  G  GP G  G  G         + GEPG  GE    G++GSPG  G  GS GE GPPG  GP G  G PG  G+ G +GP G +G  G+
Sbjct:  326 GNRGPQGMTGLPGFQGPIGYRGVKGDPGSNGDAGFIGPRGLRGMRGIPGPPGRSGTDGERGSNGLTGPKGRSGDIGMQGLPGNKGEKGYRGGRGSKGSLGRRGELGASGRPGDVGPPGENGPPGFQGPRGPPGPQGIRGLSGINGPPGPKGEIGEPGEIGSFGPGGPNGIQGPMGPQGGRGLSGPSGPRGIPGPQGVQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTG---------PPGYKGARGIRGSSGAKG---DHGPSGQVGT---RGEVGMKGVKGEYGIQGPRGPEGI------------------------------AGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEKGDPGIAGTPGGNGPVGPIGSPGPRGGPGPSGTLGSKGEQGPGGPTGMPGPVGPVGPRGTIGFIGVSGPPGTSGRPGPPGYPGERG---------ETGEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQ 816          

HSP 7 Score: 87.8113 bits (216), Expect = 7.448e-17
Identity = 197/529 (37.24%), Postives = 245/529 (46.31%), Query Frame = 2
Query: 2489 GPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSR---GPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGT---------IGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDG 4039
            GP G RG  G+PG PG  G +G  G  G  GP+G+SG+ G QG+ G +G+ G  G  GSKG  G+ G  G +G  G+                         G  G  G  G  GP+GP G +G+RGL G  G  GP G  GE G++G  G  G  G +G +G +G  G +G  G R   GPQGV G  G PG+ G         +QG  G  G  G  G PG+ G  G  G KG  G+ G +GA    G+ GP G+    G  G+ G+KG KG+ GI GPRG +G+                               GV+G++GIQG  G +G+ G  G  G VG                            PG  G  G++G  G  G QG  GE+GD   PGIAG  G    NGP+G +G  GPRG PGP G +G  G  GP G  G+ G VG  G +G     G+ G PGT          G  G  GEPG PG  GP G +G+PG  GE G MG  GP G  G
Sbjct:  362 GPRGLRGMRGIPGPPGRSGTDGERGSNGLTGPKGRSGDIGMQGLPGNKGEKGYRGGRGSKGSLGRRGELGASGRPGD------------------------VGPPGENGPPGFQGPRGPPGPQGIRGLSGINGPPGPKGEIGEPGEIGSFGPGGPNGIQGPMGPQGGRGLSGPSGPRGIPGPQGVQGSAGLPGNPG---------QQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAK---GDHGPSGQV---GTRGEVGMKGVKGEYGIQGPRGPEGI------------------------------AGVKGDIGIQGETGPRGKDGNKGDEGSVG---------------------------PPGYAGNPGIKGSRGPVGNQGRDGEKGD---PGIAGTPGG---NGPVGPIGSPGPRGGPGPSGTLGSKGEQGPGGPTGMPGPVGPVGPRGTIGFIGVSGPPGTSGRPGPPGYPGERGETGEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRG 788          

HSP 8 Score: 87.8113 bits (216), Expect = 7.901e-17
Identity = 223/564 (39.54%), Postives = 266/564 (47.16%), Query Frame = 2
Query: 2504 RGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTG---------------------------------------ILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXX---XXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRG---PPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKG---ETGLMGPIGPVGEDGE 4042
            RG SG+ G PG  G+ G  G  G  GP G +G  GP G +G RG SGP+G  G  G +G  G  G  G+ G++GD G +G  GP+GP G                           E G  G  G KG+ G  G  G KGD GP G+ G   TRGEVG+KG +G+ G+ G RGP+G++G     GD G+ GE G +G                        + G KGD G +GP    G PG  G  GP G  G  G +G PG+ GT G  G                                        +GPRG  G  G  GP G+ G  G  G PG +G TG  G  G  G +G+QG PG+ GE G +G  GP GT GPSG  G PG  G  G     GD GP G  G PG  G++G  G SG  GE G +GDRG   P G  G+ G+RG  G  G  GP GP G  G  G  G  G  G  G+ G  G +GE   KG R L G KGS G IG  G KG+ GP G TG +G RG  G KG   +TGLMG  GP G  GE
Sbjct:  463 RGLSGINGPPGPKGEIGEPGEIGSFGPGGPNGIQGPMGPQGGRGLSGPSGPRGIPGPQGVQGSAGLPGNPGQQGDMGEKGDGGPSGPPG---------------------------EMGTTGPPGYKGARGIRGSSGAKGDHGPSGQVG---TRGEVGMKGVKGEYGIQGPRGPEGIAG---VKGDIGIQGETGPRG------------------------KDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEKGDPGIAGTPGGNGPVGPIGSPGPRGGPGPSGTLGSKGEQGPGGPTGMPGPVGPVGPRGTIGFIGVSGPPGTSGRPGPPGYPGERGETGEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERGDPGSQGPPGPAGPNGPIGVAGSPGDKGGKGVVGNPGPQGPIGE---KGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGE 966          

HSP 9 Score: 87.4261 bits (215), Expect = 8.966e-17
Identity = 228/581 (39.24%), Postives = 269/581 (46.30%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKF------GPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPD---GEKGDV------GPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEK---------------GVKGDIGLIGPTGAPG---------------RIGEAGPPGK---TGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGR---KGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKA------GLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGP------QGKRGNPGPKGETGLMGPIGPVGEDG 4039
            RG  G  GP G  G+ G  G+ GS GP G   P G  G  GPQG RG  G SGP G  G +G +G        G QG  G+KG+ G  G  G  G  GP G  G  G  G  G KG  G  G  G  GE G++G+ G  G  GP GP+   G KGD+      GP GK G KG  G VG  G  G  G+ GSRGP G  G  G  GD G+ G  G  G  G  G  GP G  G  G  G +GE+               G +G IG IG +G PG                 GE G PG+    G  G+ GQPG +G+ G+ G  G RG  GL G  G  G +G  G +G  G +G +GN GPQG+ G  G  GP G    KG+ G  G  GP G  G  G  G+ GPPG +G  G  G  GSPG  G        G QGP G  G +G  G +G  GP G  G  G  GP G  G+                   G  G  G +G +GK G  G +G + + G  GSPG  G  G KGE G  G  GP      QG  G PGP+GE G+ G IG +G  G
Sbjct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

HSP 10 Score: 67.0106 bits (162), Expect = 1.419e-10
Identity = 53/117 (45.30%), Postives = 68/117 (58.12%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVG---TRGEVGMKGVKGEYGIQGPRGPEGIA---GVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSR---------GPVGNQGRDGE 2134
            KG+ G  G  G KGD GP G+ G   TRGEVG+KG +G+ G+ G RGP+G++   G  GD G+ GE G +GK G++G  G VG  G  G PG +G +         GP G  GR GE
Sbjct:  913 KGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQGIPGQPGPRGEKGVKGDIGLIGPTGAPGRIGE 1029          

HSP 11 Score: 63.5438 bits (153), Expect = 1.663e-9
Identity = 122/357 (34.17%), Postives = 150/357 (42.02%), Query Frame = 2
Query: 3005 LMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLK---------------GIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVG 4030
            L G+RGPQG++G PG  G  G                             G +G+ G  GD G IGP G  G  G  GPPG++G  G  G  G+ G KG +G +G +G  G                           N G +G  G  G +G  GR+GELG  G PG VG  G +G  G  GP G  G  G  G  G  G PG  G  G  G  G+ G  G  G +GP G  G  G  GP+GP GI                   G  G +GS GL G  G+ G  GE+                  G KG+ G  G+ G+KG+ GP G  G +G+ G  G KGE G+ GP GP G
Sbjct:  324 LKGNRGPQGMTGLPGFQGPIGYR---------------------------GVKGDPGSNGDAGFIGPRGLRGMRGIPGPPGRSGTDGERGSNGLTGPKGRSGDIGMQGLPG---------------------------NKGEKGYRGGRGSKGSLGRRGELGASGRPGDVGPPGENGPPGFQGPRGPPGPQGIRGLSGINGPPGPKGEIGEPGEIGSFGPGGPNGIQGPMGPQGGRGLSGPSGPRGI------------------PGPQGVQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG 608          

HSP 12 Score: 63.5438 bits (153), Expect = 1.779e-9
Identity = 202/540 (37.41%), Postives = 240/540 (44.44%), Query Frame = 2
Query: 2576 LGP---RGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGP---TGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTG------------------ILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRK------------GELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGER---------------YLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
             GP   RG SG  GP G +G  G+ G  G  G  G  G  GP GP G +G  G  G +G PGP G  G  G+ G+PG +G  G  G+ GP GP GE G  G  G KG+ G  G  G KGD GP G+    GTRGEVG+KG +G+ G+ G RGP+G++   G  GD G+                         G+ GPRG+ G KGD G +GP    G PG  G  GP G  G  G +G PG+ GT G  G                   LG +GEQ               G  G  G  G  G  GP+G  G +G+ GPPG              GE G  G PG  G  G  G  G PG  G  G+ G  G  G  GLPGK G+ G  G  G +G+ G RG  G PG  G  G RG +GP G +                     G KG RG  G  G  G KGER                  G+ GSPG  G  G  G PGP    GP G++G  G  G  G +GPIGP GE G+
Sbjct:  457 PGPQGIRGLSGINGPPGPKGEIGEPGEIGSFGPGGPNGIQGPMGPQGGRGLSGPSGPRGIPGPQGVQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAKGDHGPSGQV---GTRGEVGMKGVKGEYGIQGPRGPEGIA---GVKGDIGIQ------------------------GETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEKGDPGIAGTPGGNGPVGPIGSPGPRGGPGPSGTLGSKGEQ---------------GPGGPTGMPGPVGPVGPRGTIGFIGVSGPPGTSGRPGPPGYPGERGETGEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGEL---------------------GSKGDRGKFGPQGPTGDKGERGDPGSQGPPGPAGPNGPIGVAGSPGDKGGKGVVGNPGP---QGPIGEKGVRGLLGRKGSVGPIGPDGEKGD 927          

HSP 13 Score: 60.077 bits (144), Expect = 1.931e-8
Identity = 46/102 (45.10%), Postives = 60/102 (58.82%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            KG+ G  G  G++G  G SG+ G+ G+ G+ G KGE G QG +GP G  GV+G   I G+ GPRG+ G KGD G +GP G  G  G  G  G  G  G +G+
Sbjct:  946 KGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQG---IPGQPGPRGEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQ 1044          

HSP 14 Score: 60.077 bits (144), Expect = 2.066e-8
Identity = 47/110 (42.73%), Postives = 62/110 (56.36%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQG---PRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSR------GPVGNQGRDG 2131
            +G +G+ G  G+ GD G +G+ G +G+ G +G  G  G+QG     GP G  GVKGDIG+ G TG  G+ G  G  G  G  G  G PG+KG++      GP G QG DG
Sbjct:  958 RGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQGIPGQPGPRGEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDG 1067          

HSP 15 Score: 56.9954 bits (136), Expect = 1.484e-7
Identity = 180/550 (32.73%), Postives = 225/550 (40.91%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQ---SGNPGP------------------------QGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDV------------------GPLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPP---------GRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKR 3973
            +G  G  G  GLPG  G  G  G  G KG LG RG+   SG PG                         +G+ G  G  GP GE+G  G+ G FGP GP G +G                                G  G  G  GP G +G+ G  G +GS G  G  G++GD+                  GP G  G +G RG  G KG  G +G +G+RG  G+ G  G  G  G  G +G                             GVKGDIG+ G TG  G+ G  G  G  G  G  G PG+KG++G  G  G  GE+G  G  G  G  G  G  G PG +G  G SG  G +GE G  GP          G +G +G IG+ GP GTSG  G  G PG  G +G+ G  G  G  G+ G  G  G  G  G +G  G RG  G PG  G  G+ G  GP G +                           G  G+ G PG +G R  +G  G  G +G+ G +G+ GP G TG +G+R
Sbjct:  394 KGRSGDIGMQGLPGNKGEKGYRGGRGSKGSLGRRGELGASGRPGDVGPPGENGPPGFQGPRGPPGPQGIRGLSGINGPPGPKGEIGEPGEIGSFGPGGPNGIQGPM------------------------------GPQGGRGLSGPSGPRGIPGPQGVQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIA---------------------------GVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEKGDPGIAGTPGGNGPVGPIGSPGPRGGPGPSGTLGSKGEQGPGGPTGMPGPVGPVGPRGTIGFIGVSGPPGTSGRPGPPGYPGERGETGEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRGPSGLPGKPGIDGKEGSIGPKGDL---------------------------GPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGER 859          

HSP 16 Score: 56.6102 bits (135), Expect = 2.224e-7
Identity = 48/121 (39.67%), Postives = 59/121 (48.76%), Query Frame = 2
Query: 1844 IRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPE---------GIAGVKGDIGIQGETGPRGKDGNKGDEG---------------SVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            IRG SG  G  GP G++G  GE+G  G  G  GIQGP GP+         G  G+ G  G+QG  G  G  G +GD G               + GPPGY G  GI+GS G  G+ G  G+
Sbjct:  462 IRGLSGINGPPGPKGEIGEPGEIGSFGPGGPNGIQGPMGPQGGRGLSGPSGPRGIPGPQGVQGSAGLPGNPGQQGDMGEKGDGGPSGPPGEMGTTGPPGYKGARGIRGSSGAKGDHGPSGQ 582          

HSP 17 Score: 55.4546 bits (132), Expect = 4.787e-7
Identity = 44/93 (47.31%), Postives = 51/93 (54.84%), Query Frame = 2
Query: 1859 GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  G  G  G+ G +G  G  G +GE G +G RGP GIAG  G  G  G  G  G DG +G  G +GP G  GNPG KGSRG  G  G  G+K
Sbjct: 1148 GPVGSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVGPDGPRGQPGPIGPMGPIGNPGPKGSRGLDGKVGEPGQK 1240          

HSP 18 Score: 55.0694 bits (131), Expect = 7.053e-7
Identity = 44/100 (44.00%), Postives = 54/100 (54.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGN------PGIKGSRGPVG 2113
            G  G  G  G  G  G +G  G  G+ G+KG KG+ GI GPRG +G+ G  G IG +G  G RGK G +G  G+ GP G  G       PG KG  GP+G
Sbjct: 1019 GPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDGPIGPIGSKGLKGGRGKPGRQGSTGNSGPQGVEGELGIQGPPGRKGELGPIG 1118          

HSP 19 Score: 53.5286 bits (127), Expect = 1.812e-6
Identity = 46/101 (45.54%), Postives = 52/101 (51.49%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G RG  G KGD G  G  G  G +G  G  G+ G  G  G  G+ G KGD GI G  G +G DG  G  GS G  G  G PG +GS G  G QG +GE
Sbjct: 1001 GQPGPRGEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDGPIGPIGSKGLKGGRGKPGRQGSTGNSGPQGVEGE 1101          

HSP 20 Score: 53.5286 bits (127), Expect = 1.890e-6
Identity = 45/104 (43.27%), Postives = 57/104 (54.81%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGP---EGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G  G +GA G  G  G  GT+G+ G+ G +GE G+ GP GP   +G+ G +G  G QG TG  G  G +G+ G  GPPG  G  G  G  GPVG  G  G+
Sbjct: 1028 GEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDGPIGPIGSKGLKGGRGKPGRQGSTGNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTSGPSGK 1131          

HSP 21 Score: 53.1434 bits (126), Expect = 2.738e-6
Identity = 47/105 (44.76%), Postives = 57/105 (54.29%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKG---------DEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            GS G KG  G  G+ G+ G  G +GV+GE GIQGP G +G  G  G  G  G +GP GKDG  G         D+G VG PG  G  G++G  G  G QG  GE+
Sbjct: 1073 GSKGLKGGRGKPGRQGSTGNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTSGPSGKDGAPGPPGGSGQAGDDGPVGSPGLPGKAGLQGPAGGSGTQGEAGER 1177          

HSP 22 Score: 52.373 bits (124), Expect = 3.933e-6
Identity = 45/108 (41.67%), Postives = 58/108 (53.70%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPE------GIAGVKGDIGIQGETGPRGKDGN------KGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G  GA G++GP G  G+ G+ G +G  GE G  G RGP       GI G +G IG +G+ GPRG+ GN      +G  G  GP G  G+ G +G  GP G  G  GE+
Sbjct:  752 GEPGAPGENGPVGMQGSPGQPGEQGSMGERGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGER 859          

HSP 23 Score: 51.2174 bits (121), Expect = 8.831e-6
Identity = 42/100 (42.00%), Postives = 54/100 (54.00%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDG 2131
            G +G+RG  G KG  GP G  G +G+VG     G+ G++G RG  GI G +G  G+ G  GP+G  G  G  G  G  G  G  G +GS+GP G  G  G
Sbjct:  902 GEKGVRGLLGRKGSVGPIGPDGEKGDVG---PLGKTGLKGTRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNGEKGEQGKQGSQGPTGPVGVQG 998          
BLAST of Collagen type IV alpha 2 chain vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 278.485 bits (711), Expect = 5.875e-75
Identity = 270/874 (30.89%), Postives = 382/874 (43.71%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLP--GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGP---IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA--GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG------PIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE---------VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            RG PG  G  G+   G+ G+ G +G  G KG+ G  G  GNPG  G  G RG+ G  G  G KG++G+ G QG  G+ G                                             E G+ G+LG+ G+ G  G  G KG  G +GK+G KG  G        GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+G                               +G +G  GI+G PG +GE+G    IGLPG  G  G                        +PG  G  GL GP G  G +G A  G +G +G  G+ G++G++G  GP G                          G+ G+ G+VG  G+ G++ L  I    G IG  G+ G  GP GL G +G RG  G  GE G +G       IG +GE G+ G  G  G  G                             ++ N  +     +   T+  +F  +      ++  +   +   R   G + +    +C D++LQ         ++DPNEG+  DS +  C  SE          + TC+ S  + G L  Y KP   ET WQ+ LR++G +   P+L   Y   +Q+NFL+ ++  + QE+EFLC++S++YGD  L  ++  +YAT   T D   IDLSKG+ LGP  G+MDKR+Y  E GR IFNVTI YD C ++ PG+ +K  L T    MLPI+D++I  +N+ G   LG N+   CF
Sbjct: 1803 RGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMG---------------------------------------------EIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEKG------------------------------TKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRG------------------------TPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKG------------------------ENGNPGIPGEVGIKGEIGDKGLSVI----GKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRGTFILKNLAKE-------------------QLSNISKSLITIFNEETKIINFTQVKIDAVSRMKTVLQSVSSYRSNRGSSSNFPLNSCQDMQLQITIELPEAVWLDPNEGNSDDSFKGACKKSEYKYSAETIKPLLTCVRSSYEAGDLLTYLKPYDSETTWQSELRVDGLVLYPPNLRKGYPHISQLNFLQMRSEYAEQEVEFLCEESIVYGDV-LDNKDSLRYATAFQTFDDNYIDLSKGKSLGPGYGFMDKRIYLFENGRSIFNVTILYDECKFKAPGTFTKMLLSTNKPDMLPIVDYRIKYFNDNGKCMLGANIYPACF 2493          

HSP 2 Score: 113.235 bits (282), Expect = 1.671e-24
Identity = 166/534 (31.09%), Postives = 222/534 (41.57%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT-----GPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G  G  G  G PG+ GI G    +  KG+ G +G  G PG +G +G  G  G  G LG  G+RGK G +G  G KGE+                              G +G  G +G +GE G+ G+LG+ G+ G  G  G KG  G +GK+G KG  G        GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+                              G +G +G  GI+G PG +GE+G +GL                                  GLPG++GL+G  G  G +G  G  GD GP G A   G +G  G +G+                                 +G+ G+KG+R  KG  G+PG  G VG KGE G  GL+     G  G  GN GPKG  G+ GP GP+G  GE
Sbjct: 1794 KGEKGDIGFRGFPGENGIKG----VAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEK------------------------------GEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEK------------------------------GTKGAKGLPGIEGEPGDQGEIGAMGL---------------------------------IGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALP-GIKGVKGEMGL---------------------------------IGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGE 2160          

HSP 3 Score: 94.3597 bits (233), Expect = 8.746e-19
Identity = 138/400 (34.50%), Postives = 177/400 (44.25%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGL--MGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGR---KGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE----RGDRGPPGIA------GNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT---GPQGKRGNPGPKGETGLMGPIG 4021
            G KG+VG  G  G KGD+G  G  G KG +G++G +G  G+ G+  +  +G  GV G  G PG                                     KG KG+ G IG  G PG +G AG  GK G  G  GQ   KG KG+ G  G RGE G                             G  G+ G++G  G PG+   KG  G +G  GP G                           + G PGK  ++G  G  G++G  G+    RG +G PG+A      GN+G +G  G +GI G  G  G+ G +G  G IG PG  G +GL G++G+PG+KG    KG KG PG  G  G +GE G  GL    G  G RG PGP+G+ GL GPIG
Sbjct: 1768 GEKGTVGSKGHPGAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPG------------------------------------YKGEKGNTG-IG--GNPGLLGFAGNRGKKGVKGNNGQ---KGEKGEIGRQGNRGEMG---------------------------EIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKG---------------------------NSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGT---KGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHGPIG 2068          

HSP 4 Score: 88.9669 bits (219), Expect = 3.640e-17
Identity = 143/413 (34.62%), Postives = 184/413 (44.55%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPL---GKTGLKG--TRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQ---------------GVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERY--LKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVG 4030
            G +GL G+K         GEKGD+G     G+ G+KG   +GE G++GS+G  G  G +G  G+ G PG  G AG                      +G  G  G  G+KG KG+IG  G  G  G IG  G  GK G SG  G+ G+KG KG+ G  GP+G  G   P              K  +  S  N GPQ               GV+GE G+ G  G KG++G  G  G  G                             G PGK G+QG  G  G  GE G +G +G PGI G  G +G  G +G++         G  G  G  G PGP+G  GL G +G+ G +G     +KG+KG  G IG +G KG+       GP+G+ GNPG  GE G+ G IG  G
Sbjct: 1786 GEKGLPGKK---------GEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAG---------------------NRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSG--TPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLI---------------------------GEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLI---------GLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDR------GPKGENGNPGIPGEVGIKGEIGDKG 2124          

HSP 5 Score: 64.3142 bits (155), Expect = 9.680e-10
Identity = 47/109 (43.12%), Postives = 60/109 (55.05%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQ---------VGTRGEVGMKGVK-----GEYGI---QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            KG  G+ G  G KGD GP G+         VG +GE+G KG+      G+YG     GP+G EGI G +G IG+ GE G  G +G KG+ G +G  G  G PG+ G RG
Sbjct: 2084 KGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRG 2192          

HSP 6 Score: 62.3882 bits (150), Expect = 3.718e-9
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 2
Query: 1838 RGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAG--VKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            RG +G+ G+KG  G  G +G +G  G KG KG+ G +G  G  GI G  VKG+ G+QG  G  G  G KG+ G  G PG  G  G +G +G  GN G+ GEK
Sbjct: 1767 RGEKGTVGSKGHPGAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEK 1868          

HSP 7 Score: 61.6178 bits (148), Expect = 6.107e-9
Identity = 52/129 (40.31%), Postives = 73/129 (56.59%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRG------EVGMKGVKGEYGI---QGPRGPEGIAGVKGD------------IGIQGETGPRGKDGNKGDEGSVGPPGYAG-----NPGIKGSRGPVGNQGRDGEK 2137
            KG +G+ G SG KGD G SG++G  G      ++G++G+KGE G    +G +G +G+ G++G+            IG+ GE+G RG  G +GDEG  GP G  G      PGIKG +G +G  G  GEK
Sbjct: 1968 KGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEK 2096          

HSP 8 Score: 61.6178 bits (148), Expect = 6.756e-9
Identity = 49/117 (41.88%), Postives = 66/117 (56.41%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD---HGPSGQVGTRGEV--GMKGVKGEYGI---------QGPRGPEGIAGVKGDIGIQGETGPRGKD--GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G+RG+ G +GD   HGP G  G RG    G+KGVKGE G+         +GP+G  G  G+ G++GI+GE G +G    G  G  G++G  G  G  GI+G RGP+G  G  G+
Sbjct: 2047 GESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGK 2163          

HSP 9 Score: 59.3066 bits (142), Expect = 3.070e-8
Identity = 49/124 (39.52%), Postives = 63/124 (50.81%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKG---EYGIQG-------PRGPEGIAGV------------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +GI+G  G  G  GP G  GT G++ +KG+KG     GIQG       P+G  G+AG+            KG +GI GE G  GK G  G +G +G PG  G  G+KG  G  G +G  G K
Sbjct: 1899 GKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAK 2022          

HSP 10 Score: 58.151 bits (139), Expect = 8.075e-8
Identity = 46/115 (40.00%), Postives = 62/115 (53.91%), Query Frame = 2
Query: 1829 KGARGIRGSS----------GAKGDHGPSGQVGTRGEVGMKGV---KGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            KG++GI+G S          G  G  G +G +G +GE G+ G+   KG+ G  G  G +G+ G  G IGIQG  G  G+ G KG +G+ G PG  G PG +G  G +G  G  GE
Sbjct: 1934 KGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGE 2048          

HSP 11 Score: 56.6102 bits (135), Expect = 2.330e-7
Identity = 44/130 (33.85%), Postives = 66/130 (50.77%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTR---GEVGMKGVKGEYGIQGPRGPEGIAGVKGDI------------------GIQGETGPRGKDGN-------KGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G +G++G++G KG+ G  G+ G R   GE+G+ G+ G+ G  G  G +GI G KG++                  GI+G  G +G  G        +GD G  G PG  GN G+KG +G +G  G  G+
Sbjct: 1853 RGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGD 1982          

HSP 12 Score: 55.8398 bits (133), Expect = 3.731e-7
Identity = 47/108 (43.52%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG---IAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPG---IKGSRGPVGNQGRDGE 2134
            KG  GI G+ G  G  G  G+ G +G  G KG KGE G QG RG  G   + G+ G IG  G+ G +G  G KG+ G  GP G +G PG   IKG +G  G++G  G+
Sbjct: 1835 KGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQ 1942          

HSP 13 Score: 53.1434 bits (126), Expect = 2.333e-6
Identity = 44/112 (39.29%), Postives = 60/112 (53.57%), Query Frame = 2
Query: 1826 YKGARGIRGSS--GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY------AGNPGIKGSRGPVGNQGRDGEK 2137
            + G  GI+G +  G  G  G  G  G +GE G  G+ G  G+ G  G  G  GVKG+ G +GE G  G+ GN+G+ G +G PG       +G PG +G +G  GN G+ G K
Sbjct: 1805 FPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPK 1916          

HSP 14 Score: 52.373 bits (124), Expect = 4.769e-6
Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK------DGNKGDEGSVGPPGYAGNP---GIKGSRGPVGNQGRDG 2131
            G+ G KG+ G  G  G +G++G  G  G+ G+ G  G  GI G+KG+IG  GE G +G       +G  GD+G +G  G  G P   G++G+ GP G++G  G
Sbjct: 1963 GNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHG 2065          

HSP 15 Score: 52.373 bits (124), Expect = 5.016e-6
Identity = 45/106 (42.45%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKG-----VKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            GA+G  G  G  G  G  G +G RG  G  G     VKGE G+QG +G  G  G KG+ GI G  G  G  GN+G +      G  GN G KG +G +G QG  GE
Sbjct: 1780 GAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKK------GVKGNNGQKGEKGEIGRQGNRGE 1879          
BLAST of Collagen type IV alpha 2 chain vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 278.1 bits (710), Expect = 6.522e-75
Identity = 270/874 (30.89%), Postives = 382/874 (43.71%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLP--GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGP---IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA--GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG------PIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE---------VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKGISTLGLNVLSMCF 5011
            RG PG  G  G+   G+ G+ G +G  G KG+ G  G  GNPG  G  G RG+ G  G  G KG++G+ G QG  G+ G                                             E G+ G+LG+ G+ G  G  G KG  G +GK+G KG  G        GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+G                               +G +G  GI+G PG +GE+G    IGLPG  G  G                        +PG  G  GL GP G  G +G A  G +G +G  G+ G++G++G  GP G                          G+ G+ G+VG  G+ G++ L  I    G IG  G+ G  GP GL G +G RG  G  GE G +G       IG +GE G+ G  G  G  G                             ++ N  +     +   T+  +F  +      ++  +   +   R   G + +    +C D++LQ         ++DPNEG+  DS +  C  SE          + TC+ S  + G L  Y KP   ET WQ+ LR++G +   P+L   Y   +Q+NFL+ ++  + QE+EFLC++S++YGD  L  ++  +YAT   T D   IDLSKG+ LGP  G+MDKR+Y  E GR IFNVTI YD C ++ PG+ +K  L T    MLPI+D++I  +N+ G   LG N+   CF
Sbjct: 1808 RGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMG---------------------------------------------EIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEKG------------------------------TKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRG------------------------TPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKG------------------------ENGNPGIPGEVGIKGEIGDKGLSVI----GKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRGTFILKNLAKE-------------------QLSNISKSLITIFNEETKIINFTQVKIDAVSRMKTVLQSVSSYRSNRGSSSNFPLNSCQDMQLQITIELPEAVWLDPNEGNSDDSFKGACKKSEYKYSAETIKPLLTCVRSSYEAGDLLTYLKPYDSETTWQSELRVDGLVLYPPNLRKGYPHISQLNFLQMRSEYAEQEVEFLCEESIVYGDV-LDNKDSLRYATAFQTFDDNYIDLSKGKSLGPGYGFMDKRIYLFENGRSIFNVTILYDECKFKAPGTFTKMLLSTNKPDMLPIVDYRIKYFNDNGKCMLGANIYPACF 2498          

HSP 2 Score: 112.849 bits (281), Expect = 1.729e-24
Identity = 166/534 (31.09%), Postives = 222/534 (41.57%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT-----GPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G  G  G  G PG+ GI G    +  KG+ G +G  G PG +G +G  G  G  G LG  G+RGK G +G  G KGE+                              G +G  G +G +GE G+ G+LG+ G+ G  G  G KG  G +GK+G KG  G        GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+                              G +G +G  GI+G PG +GE+G +GL                                  GLPG++GL+G  G  G +G  G  GD GP G A   G +G  G +G+                                 +G+ G+KG+R  KG  G+PG  G VG KGE G  GL+     G  G  GN GPKG  G+ GP GP+G  GE
Sbjct: 1799 KGEKGDIGFRGFPGENGIKG----VAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEK------------------------------GEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEK------------------------------GTKGAKGLPGIEGEPGDQGEIGAMGL---------------------------------IGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALP-GIKGVKGEMGL---------------------------------IGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGE 2165          

HSP 3 Score: 94.3597 bits (233), Expect = 9.049e-19
Identity = 138/400 (34.50%), Postives = 177/400 (44.25%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGL--MGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGR---KGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE----RGDRGPPGIA------GNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT---GPQGKRGNPGPKGETGLMGPIG 4021
            G KG+VG  G  G KGD+G  G  G KG +G++G +G  G+ G+  +  +G  GV G  G PG                                     KG KG+ G IG  G PG +G AG  GK G  G  GQ   KG KG+ G  G RGE G                             G  G+ G++G  G PG+   KG  G +G  GP G                           + G PGK  ++G  G  G++G  G+    RG +G PG+A      GN+G +G  G +GI G  G  G+ G +G  G IG PG  G +GL G++G+PG+KG    KG KG PG  G  G +GE G  GL    G  G RG PGP+G+ GL GPIG
Sbjct: 1773 GEKGTVGSKGHPGAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPG------------------------------------YKGEKGNTG-IG--GNPGLLGFAGNRGKKGVKGNNGQ---KGEKGEIGRQGNRGEMG---------------------------EIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKG---------------------------NSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGT---KGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHGPIG 2073          

HSP 4 Score: 88.9669 bits (219), Expect = 3.830e-17
Identity = 143/413 (34.62%), Postives = 184/413 (44.55%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPL---GKTGLKG--TRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQ---------------GVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERY--LKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIGPVG 4030
            G +GL G+K         GEKGD+G     G+ G+KG   +GE G++GS+G  G  G +G  G+ G PG  G AG                      +G  G  G  G+KG KG+IG  G  G  G IG  G  GK G SG  G+ G+KG KG+ G  GP+G  G   P              K  +  S  N GPQ               GV+GE G+ G  G KG++G  G  G  G                             G PGK G+QG  G  G  GE G +G +G PGI G  G +G  G +G++         G  G  G  G PGP+G  GL G +G+ G +G     +KG+KG  G IG +G KG+       GP+G+ GNPG  GE G+ G IG  G
Sbjct: 1791 GEKGLPGKK---------GEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAG---------------------NRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSG--TPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLI---------------------------GEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLI---------GLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDR------GPKGENGNPGIPGEVGIKGEIGDKG 2129          

HSP 5 Score: 64.3142 bits (155), Expect = 9.601e-10
Identity = 47/109 (43.12%), Postives = 60/109 (55.05%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQ---------VGTRGEVGMKGVK-----GEYGI---QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            KG  G+ G  G KGD GP G+         VG +GE+G KG+      G+YG     GP+G EGI G +G IG+ GE G  G +G KG+ G +G  G  G PG+ G RG
Sbjct: 2089 KGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRG 2197          

HSP 6 Score: 62.3882 bits (150), Expect = 3.782e-9
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 2
Query: 1838 RGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAG--VKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            RG +G+ G+KG  G  G +G +G  G KG KG+ G +G  G  GI G  VKG+ G+QG  G  G  G KG+ G  G PG  G  G +G +G  GN G+ GEK
Sbjct: 1772 RGEKGTVGSKGHPGAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEK 1873          

HSP 7 Score: 61.6178 bits (148), Expect = 6.108e-9
Identity = 52/129 (40.31%), Postives = 73/129 (56.59%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRG------EVGMKGVKGEYGI---QGPRGPEGIAGVKGD------------IGIQGETGPRGKDGNKGDEGSVGPPGYAG-----NPGIKGSRGPVGNQGRDGEK 2137
            KG +G+ G SG KGD G SG++G  G      ++G++G+KGE G    +G +G +G+ G++G+            IG+ GE+G RG  G +GDEG  GP G  G      PGIKG +G +G  G  GEK
Sbjct: 1973 KGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEK 2101          

HSP 8 Score: 61.6178 bits (148), Expect = 6.930e-9
Identity = 49/117 (41.88%), Postives = 66/117 (56.41%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD---HGPSGQVGTRGEV--GMKGVKGEYGI---------QGPRGPEGIAGVKGDIGIQGETGPRGKD--GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G+RG+ G +GD   HGP G  G RG    G+KGVKGE G+         +GP+G  G  G+ G++GI+GE G +G    G  G  G++G  G  G  GI+G RGP+G  G  G+
Sbjct: 2052 GESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGK 2168          

HSP 9 Score: 59.3066 bits (142), Expect = 3.096e-8
Identity = 49/124 (39.52%), Postives = 63/124 (50.81%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKG---EYGIQG-------PRGPEGIAGV------------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +GI+G  G  G  GP G  GT G++ +KG+KG     GIQG       P+G  G+AG+            KG +GI GE G  GK G  G +G +G PG  G  G+KG  G  G +G  G K
Sbjct: 1904 GKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAK 2027          

HSP 10 Score: 58.151 bits (139), Expect = 8.009e-8
Identity = 48/115 (41.74%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 1829 KGARGIRGSS----GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---------PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            KG++GI+G S    G +GD G +G  G  G +G+KG KG  GI G +G          +G+ G  G IGIQG  G  G+ G KG +G+ G PG  G PG +G  G +G  G  GE
Sbjct: 1939 KGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGE 2053          

HSP 11 Score: 56.6102 bits (135), Expect = 2.350e-7
Identity = 44/130 (33.85%), Postives = 66/130 (50.77%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTR---GEVGMKGVKGEYGIQGPRGPEGIAGVKGDI------------------GIQGETGPRGKDGN-------KGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G +G++G++G KG+ G  G+ G R   GE+G+ G+ G+ G  G  G +GI G KG++                  GI+G  G +G  G        +GD G  G PG  GN G+KG +G +G  G  G+
Sbjct: 1858 RGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGD 1987          

HSP 12 Score: 55.8398 bits (133), Expect = 3.827e-7
Identity = 47/108 (43.52%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG---IAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPG---IKGSRGPVGNQGRDGE 2134
            KG  GI G+ G  G  G  G+ G +G  G KG KGE G QG RG  G   + G+ G IG  G+ G +G  G KG+ G  GP G +G PG   IKG +G  G++G  G+
Sbjct: 1840 KGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQ 1947          

HSP 13 Score: 53.1434 bits (126), Expect = 2.334e-6
Identity = 44/112 (39.29%), Postives = 60/112 (53.57%), Query Frame = 2
Query: 1826 YKGARGIRGSS--GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY------AGNPGIKGSRGPVGNQGRDGEK 2137
            + G  GI+G +  G  G  G  G  G +GE G  G+ G  G+ G  G  G  GVKG+ G +GE G  G+ GN+G+ G +G PG       +G PG +G +G  GN G+ G K
Sbjct: 1810 FPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPK 1921          

HSP 14 Score: 52.373 bits (124), Expect = 4.810e-6
Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK------DGNKGDEGSVGPPGYAGNP---GIKGSRGPVGNQGRDG 2131
            G+ G KG+ G  G  G +G++G  G  G+ G+ G  G  GI G+KG+IG  GE G +G       +G  GD+G +G  G  G P   G++G+ GP G++G  G
Sbjct: 1968 GNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHG 2070          

HSP 15 Score: 52.373 bits (124), Expect = 4.974e-6
Identity = 45/106 (42.45%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKG-----VKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            GA+G  G  G  G  G  G +G RG  G  G     VKGE G+QG +G  G  G KG+ GI G  G  G  GN+G +      G  GN G KG +G +G QG  GE
Sbjct: 1785 GAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKK------GVKGNNGQKGEKGEIGRQGNRGE 1884          
BLAST of Collagen type IV alpha 2 chain vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 269.24 bits (687), Expect = 3.455e-72
Identity = 265/861 (30.78%), Postives = 376/861 (43.67%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLP--GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGP---IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA--GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG------PIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE---------VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKG 4972
            RG PG  G  G+   G+ G+ G +G  G KG+ G  G  GNPG  G  G RG+ G  G  G KG++G+ G QG  G+ G                                             E G+ G+LG+ G+ G  G  G KG  G +GK+G KG  G        GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+G                               +G +G  GI+G PG +GE+G    IGLPG  G  G                        +PG  G  GL GP G  G +G A  G +G +G  G+ G++G++G  GP G                          G+ G+ G+VG  G+ G++ L  I    G IG  G+ G  GP GL G +G RG  G  GE G +G       IG +GE G+ G  G  G  G                             ++ N  +     +   T+  +F  +      ++  +   +   R   G + +    +C D++LQ         ++DPNEG+  DS +  C  SE          + TC+ S  + G L  Y KP   ET WQ+ LR++G +   P+L   Y   +Q+NFL+ ++  + QE+EFLC++S++YGD  L  ++  +YAT   T D   IDLSKG+ LGP  G+MDKR+Y  E GR IFNVTI YD C ++ PG+ +K  L T    MLPI+D++I  +N+ G
Sbjct: 1327 RGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMG---------------------------------------------EIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEKG------------------------------TKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRG------------------------TPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKG------------------------ENGNPGIPGEVGIKGEIGDKGLSVI----GKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRGTFILKNLAKE-------------------QLSNISKSLITIFNEETKIINFTQVKIDAVSRMKTVLQSVSSYRSNRGSSSNFPLNSCQDMQLQITIELPEAVWLDPNEGNSDDSFKGACKKSEYKYSAETIKPLLTCVRSSYEAGDLLTYLKPYDSETTWQSELRVDGLVLYPPNLRKGYPHISQLNFLQMRSEYAEQEVEFLCEESIVYGDV-LDNKDSLRYATAFQTFDDNYIDLSKGKSLGPGYGFMDKRIYLFENGRSIFNVTILYDECKFKAPGTFTKMLLSTNKPDMLPIVDYRIKYFNDNG 2004          

HSP 2 Score: 111.694 bits (278), Expect = 3.978e-24
Identity = 166/534 (31.09%), Postives = 222/534 (41.57%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT-----GPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G  G  G  G PG+ GI G    +  KG+ G +G  G PG +G +G  G  G  G LG  G+RGK G +G  G KGE+                              G +G  G +G +GE G+ G+LG+ G+ G  G  G KG  G +GK+G KG  G        GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+                              G +G +G  GI+G PG +GE+G +GL                                  GLPG++GL+G  G  G +G  G  GD GP G A   G +G  G +G+                                 +G+ G+KG+R  KG  G+PG  G VG KGE G  GL+     G  G  GN GPKG  G+ GP GP+G  GE
Sbjct: 1318 KGEKGDIGFRGFPGENGIKG----VAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEK------------------------------GEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEK------------------------------GTKGAKGLPGIEGEPGDQGEIGAMGL---------------------------------IGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALP-GIKGVKGEMGL---------------------------------IGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGE 1684          

HSP 3 Score: 88.9669 bits (219), Expect = 3.469e-17
Identity = 142/410 (34.63%), Postives = 183/410 (44.63%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPL---GKTGLKG--TRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQ---------------GVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERY--LKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            G +GL G+K         GEKGD+G     G+ G+KG   +GE G++GS+G  G  G +G  G+ G PG  G AG                      +G  G  G  G+KG KG+IG  G  G  G IG  G  GK G SG  G+ G+KG KG+ G  GP+G  G   P              K  +  S  N GPQ               GV+GE G+ G  G KG++G  G  G  G                             G PGK G+QG  G  G  GE G +G +G PGI G  G +G  G +G++         G  G  G  G PGP+G  GL G +G+ G +G     +KG+KG  G IG +G KG+       GP+G+ GNPG  GE G+ G IG
Sbjct: 1310 GEKGLPGKK---------GEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAG---------------------NRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSG--TPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLI---------------------------GEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLI---------GLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGD------RGPKGENGNPGIPGEVGIKGEIG 1645          

HSP 4 Score: 64.3142 bits (155), Expect = 1.058e-9
Identity = 47/109 (43.12%), Postives = 60/109 (55.05%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQ---------VGTRGEVGMKGVK-----GEYGI---QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            KG  G+ G  G KGD GP G+         VG +GE+G KG+      G+YG     GP+G EGI G +G IG+ GE G  G +G KG+ G +G  G  G PG+ G RG
Sbjct: 1608 KGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRG 1716          

HSP 5 Score: 62.3882 bits (150), Expect = 3.928e-9
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 2
Query: 1838 RGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAG--VKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            RG +G+ G+KG  G  G +G +G  G KG KG+ G +G  G  GI G  VKG+ G+QG  G  G  G KG+ G  G PG  G  G +G +G  GN G+ GEK
Sbjct: 1291 RGEKGTVGSKGHPGAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEK 1392          

HSP 6 Score: 61.6178 bits (148), Expect = 6.135e-9
Identity = 49/117 (41.88%), Postives = 66/117 (56.41%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD---HGPSGQVGTRGEV--GMKGVKGEYGI---------QGPRGPEGIAGVKGDIGIQGETGPRGKD--GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G+RG+ G +GD   HGP G  G RG    G+KGVKGE G+         +GP+G  G  G+ G++GI+GE G +G    G  G  G++G  G  G  GI+G RGP+G  G  G+
Sbjct: 1571 GESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGK 1687          

HSP 7 Score: 61.6178 bits (148), Expect = 7.198e-9
Identity = 53/129 (41.09%), Postives = 69/129 (53.49%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRG------EVGMKGVKGEYG---------------IQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAG-----NPGIKGSRGPVGNQGRDGEK 2137
            KG +G+ G SG KGD G SG++G  G      ++G++G+KGE G               I+G  G +G  G  G IG+ GE+G RG  G +GDEG  GP G  G      PGIKG +G +G  G  GEK
Sbjct: 1492 KGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEK 1620          

HSP 8 Score: 59.3066 bits (142), Expect = 3.299e-8
Identity = 49/124 (39.52%), Postives = 63/124 (50.81%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKG---EYGIQG-------PRGPEGIAGV------------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +GI+G  G  G  GP G  GT G++ +KG+KG     GIQG       P+G  G+AG+            KG +GI GE G  GK G  G +G +G PG  G  G+KG  G  G +G  G K
Sbjct: 1423 GKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAK 1546          

HSP 9 Score: 58.151 bits (139), Expect = 8.115e-8
Identity = 48/115 (41.74%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 1829 KGARGIRGSS----GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---------PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            KG++GI+G S    G +GD G +G  G  G +G+KG KG  GI G +G          +G+ G  G IGIQG  G  G+ G KG +G+ G PG  G PG +G  G +G  G  GE
Sbjct: 1458 KGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGE 1572          

HSP 10 Score: 56.225 bits (134), Expect = 2.817e-7
Identity = 44/130 (33.85%), Postives = 66/130 (50.77%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTR---GEVGMKGVKGEYGIQGPRGPEGIAGVKGDI------------------GIQGETGPRGKDGN-------KGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G +G++G++G KG+ G  G+ G R   GE+G+ G+ G+ G  G  G +GI G KG++                  GI+G  G +G  G        +GD G  G PG  GN G+KG +G +G  G  G+
Sbjct: 1377 RGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGD 1506          

HSP 11 Score: 55.8398 bits (133), Expect = 3.846e-7
Identity = 47/108 (43.52%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG---IAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPG---IKGSRGPVGNQGRDGE 2134
            KG  GI G+ G  G  G  G+ G +G  G KG KGE G QG RG  G   + G+ G IG  G+ G +G  G KG+ G  GP G +G PG   IKG +G  G++G  G+
Sbjct: 1359 KGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQ 1466          

HSP 12 Score: 53.1434 bits (126), Expect = 2.366e-6
Identity = 44/112 (39.29%), Postives = 60/112 (53.57%), Query Frame = 2
Query: 1826 YKGARGIRGSS--GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY------AGNPGIKGSRGPVGNQGRDGEK 2137
            + G  GI+G +  G  G  G  G  G +GE G  G+ G  G+ G  G  G  GVKG+ G +GE G  G+ GN+G+ G +G PG       +G PG +G +G  GN G+ G K
Sbjct: 1329 FPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPK 1440          

HSP 13 Score: 52.373 bits (124), Expect = 4.958e-6
Identity = 45/106 (42.45%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKG-----VKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            GA+G  G  G  G  G  G +G RG  G  G     VKGE G+QG +G  G  G KG+ GI G  G  G  GN+G +      G  GN G KG +G +G QG  GE
Sbjct: 1304 GAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKK------GVKGNNGQKGEKGEIGRQGNRGE 1403          

HSP 14 Score: 51.9878 bits (123), Expect = 5.042e-6
Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK------DGNKGDEGSVGPPGYAGNP---GIKGSRGPVGNQGRDG 2131
            G+ G KG+ G  G  G +G++G  G  G+ G+ G  G  GI G+KG+IG  GE G +G       +G  GD+G +G  G  G P   G++G+ GP G++G  G
Sbjct: 1487 GNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHG 1589          
BLAST of Collagen type IV alpha 2 chain vs. Planmine SMEST
Match: SMESG000077977.1 (SMESG000077977.1)

HSP 1 Score: 265.388 bits (677), Expect = 5.811e-71
Identity = 265/861 (30.78%), Postives = 376/861 (43.67%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLP--GKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGE------VGIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGP---IGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEA--GERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMG------PIGPVGEDGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVKIRNPPEGSSGRYKRSTRAQSFVDINDALFDKVDKITNKIKRIRFPTGHNKDSAARTCADIKLQRKQSTDGQYYIDPNEGSGKDSIEVECVFSEE---------VKTCISSVKQQGGLYNYKKPMKGETNWQNSLRLEGAISRAPDLHSMYVDHTQMNFLRNQNSKSYQEIEFLCDQSVIYGDSELSEENKFKYATVLMTHDFKSIDLSKGERLGPNEGYMDKRVYKDEFGRPIFNVTIEYDGCMYRTPGSSSKFKLETTDLSMLPIIDFQINDYNNKG 4972
            RG PG  G  G+   G+ G+ G +G  G KG+ G  G  GNPG  G  G RG+ G  G  G KG++G+ G QG  G+ G                                             E G+ G+LG+ G+ G  G  G KG  G +GK+G KG  G        GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+G                               +G +G  GI+G PG +GE+G    IGLPG  G  G                        +PG  G  GL GP G  G +G A  G +G +G  G+ G++G++G  GP G                          G+ G+ G+VG  G+ G++ L  I    G IG  G+ G  GP GL G +G RG  G  GE G +G       IG +GE G+ G  G  G  G                             ++ N  +     +   T+  +F  +      ++  +   +   R   G + +    +C D++LQ         ++DPNEG+  DS +  C  SE          + TC+ S  + G L  Y KP   ET WQ+ LR++G +   P+L   Y   +Q+NFL+ ++  + QE+EFLC++S++YGD  L  ++  +YAT   T D   IDLSKG+ LGP  G+MDKR+Y  E GR IFNVTI YD C ++ PG+ +K  L T    MLPI+D++I  +N+ G
Sbjct: 1808 RGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMG---------------------------------------------EIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEKG------------------------------TKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRG------------------------TPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKG------------------------ENGNPGIPGEVGIKGEIGDKGLSVI----GKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRGTFILKNLAKE-------------------QLSNISKSLITIFNEETKIINFTQVKIDAVSRMKTVLQSVSSYRSNRGSSSNFPLNSCQDMQLQITIELPEAVWLDPNEGNSDDSFKGACKKSEYKYSAETIKPLLTCVRSSYEAGDLLTYLKPYDSETTWQSELRVDGLVLYPPNLRKGYPHISQLNFLQMRSEYAEQEVEFLCEESIVYGDV-LDNKDSLRYATAFQTFDDNYIDLSKGKSLGPGYGFMDKRIYLFENGRSIFNVTILYDECKFKAPGTFTKMLLSTNKPDMLPIVDYRIKYFNDNG 2485          

HSP 2 Score: 107.071 bits (266), Expect = 1.227e-22
Identity = 161/549 (29.33%), Postives = 212/549 (38.62%), Query Frame = 2
Query: 2486 RGPPGTRGPSGLPGKPGIDGKEGSIGPKGDLGPRGQSGNPGPQGIRGARGQSGPTGELGSKGDRGKFGPQGPTGDKGERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVRGLLGRKGSVGPIGPDGEKGDVGPL---GKTGLKGTRGEV------------------GIKGSEGKTGLMG----SRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT-----GPQGKRGNPGPKGETGLMGPIGPVGEDGE 4042
            +G  G  G  G PG+ GI G    +  KG+ G +G  G PG +G +G  G  G  G LG  G+RGK G +G  G KG                                             EKG  G  G +G +G IG  G  G +G     GK G+KG +G V                  GIKG++G  G+ G    +RGPQG  G  G PG+ G                                   GVKG+ GL+G +G  G IG++G  GK G  G  G+ G++G KG+   +G  GE+                              G +G +G  GI+G PG +GE+G +GL                                  GLPG++GL+G  G  G +G  G  GD GP G A   G +G  G +G+                                 +G+ G+KG+R  KG  G+PG  G VG KGE G  GL+     G  G  GN GPKG  G+ GP GP+G  GE
Sbjct: 1799 KGEKGDIGFRGFPGENGIKG----VAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKG---------------------------------------------EKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENG---------------------------------NIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGE---IGQPGEK------------------------------GTKGAKGLPGIEGEPGDQGEIGAMGL---------------------------------IGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALP-GIKGVKGEMGL---------------------------------IGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGE 2165          

HSP 3 Score: 92.4337 bits (228), Expect = 3.347e-18
Identity = 138/400 (34.50%), Postives = 177/400 (44.25%), Query Frame = 2
Query: 2876 GRKGSVGPIGPDGEKGDVGPLGKTGLKGTRGEVGIKGSEGKTGL--MGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQGVEGELGIQGPPGR---KGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGE----RGDRGPPGIA------GNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERYLKGIKGSPGTIGAVGSKGEPGPPGLT---GPQGKRGNPGPKGETGLMGPIG 4021
            G KG+VG  G  G KGD+G  G  G KG +G++G +G  G+ G+  +  +G  GV G  G PG                                     KG KG+ G IG  G PG +G AG  GK G  G  GQ   KG KG+ G  G RGE G                             G  G+ G++G  G PG+   KG  G +G  GP G                           + G PGK  ++G  G  G++G  G+    RG +G PG+A      GN+G +G  G +GI G  G  G+ G +G  G IG PG  G +GL G++G+PG+KG    KG KG PG  G  G +GE G  GL    G  G RG PGP+G+ GL GPIG
Sbjct: 1773 GEKGTVGSKGHPGAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPG------------------------------------YKGEKGNTG-IG--GNPGLLGFAGNRGKKGVKGNNGQ---KGEKGEIGRQGNRGEMG---------------------------EIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKG---------------------------NSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGT---KGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHGPIG 2073          

HSP 4 Score: 86.6557 bits (213), Expect = 1.631e-16
Identity = 142/410 (34.63%), Postives = 183/410 (44.63%), Query Frame = 2
Query: 2858 GVRGLLGRKGSVGPIGPDGEKGDVGPL---GKTGLKG--TRGEVGIKGSEGKTGLMGSRGPQGVSGEPGSPGDAGVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKGVKGDIGLIGPTGAPGRIGEAGPPGKTGASGAEGQPGVKGTKGDTGILGPRGEQGLDXXXXXXXXXXXXXXXXXXXXXXXXXNSGPQ---------------GVEGELGIQGPPGRKGELGPIGLPGPVGTXXXXXXXXXXXXXXXXXXXXXXXXXXSPGLPGKAGLQGPAGGSGTQGEAGERGDRGPPGIAGNMGQRGPNGPIGIVXXXXXXXXXXXXXXXXXXXXXXXXXSRGLDGKVGEPGQKGERY--LKGIKGSPGTIGAVGSKGEPGPPGLTGPQGKRGNPGPKGETGLMGPIG 4021
            G +GL G+K         GEKGD+G     G+ G+KG   +GE G++GS+G  G  G +G  G+ G PG  G AG                      +G  G  G  G+KG KG+IG  G  G  G IG  G  GK G SG  G+ G+KG KG+ G  GP+G  G   P              K  +  S  N GPQ               GV+GE G+ G  G KG++G  G  G  G                             G PGK G+QG  G  G  GE G +G +G PGI G  G +G  G +G++         G  G  G  G PGP+G  GL G +G+ G +G     +KG+KG  G IG +G KG+       GP+G+ GNPG  GE G+ G IG
Sbjct: 1791 GEKGLPGKK---------GEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAG---------------------NRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSG--TPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLI---------------------------GEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLI---------GLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGD------RGPKGENGNPGIPGEVGIKGEIG 2126          

HSP 5 Score: 64.3142 bits (155), Expect = 1.147e-9
Identity = 47/109 (43.12%), Postives = 60/109 (55.05%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQ---------VGTRGEVGMKGVK-----GEYGI---QGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRG 2104
            KG  G+ G  G KGD GP G+         VG +GE+G KG+      G+YG     GP+G EGI G +G IG+ GE G  G +G KG+ G +G  G  G PG+ G RG
Sbjct: 2089 KGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGKVGYNGTKGEIGEIGEKGDKGLPGVPGQRG 2197          

HSP 6 Score: 62.003 bits (149), Expect = 6.058e-9
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 2
Query: 1838 RGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAG--VKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            RG +G+ G+KG  G  G +G +G  G KG KG+ G +G  G  GI G  VKG+ G+QG  G  G  G KG+ G  G PG  G  G +G +G  GN G+ GEK
Sbjct: 1772 RGEKGTVGSKGHPGAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEK 1873          

HSP 7 Score: 61.6178 bits (148), Expect = 7.995e-9
Identity = 52/129 (40.31%), Postives = 73/129 (56.59%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRG------EVGMKGVKGEYGI---QGPRGPEGIAGVKGD------------IGIQGETGPRGKDGNKGDEGSVGPPGYAG-----NPGIKGSRGPVGNQGRDGEK 2137
            KG +G+ G SG KGD G SG++G  G      ++G++G+KGE G    +G +G +G+ G++G+            IG+ GE+G RG  G +GDEG  GP G  G      PGIKG +G +G  G  GEK
Sbjct: 1973 KGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEK 2101          

HSP 8 Score: 61.6178 bits (148), Expect = 7.995e-9
Identity = 49/117 (41.88%), Postives = 66/117 (56.41%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGD---HGPSGQVGTRGEV--GMKGVKGEYGI---------QGPRGPEGIAGVKGDIGIQGETGPRGKD--GNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            G  G+RG+ G +GD   HGP G  G RG    G+KGVKGE G+         +GP+G  G  G+ G++GI+GE G +G    G  G  G++G  G  G  GI+G RGP+G  G  G+
Sbjct: 2052 GESGLRGTPGPEGDEGLHGPIGDDGPRGTALPGIKGVKGEMGLIGDIGEKGDRGPKGENGNPGIPGEVGIKGEIGDKGLSVIGKIGKYGTIGNTGPKGLEGIRGPRGPIGLVGEVGK 2168          

HSP 9 Score: 58.9214 bits (141), Expect = 4.049e-8
Identity = 49/124 (39.52%), Postives = 63/124 (50.81%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKG---EYGIQG-------PRGPEGIAGV------------KGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGEK 2137
            G +GI+G  G  G  GP G  GT G++ +KG+KG     GIQG       P+G  G+AG+            KG +GI GE G  GK G  G +G +G PG  G  G+KG  G  G +G  G K
Sbjct: 1904 GKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAK 2027          

HSP 10 Score: 57.7658 bits (138), Expect = 1.074e-7
Identity = 48/115 (41.74%), Postives = 63/115 (54.78%), Query Frame = 2
Query: 1829 KGARGIRGSS----GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRG---------PEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            KG++GI+G S    G +GD G +G  G  G +G+KG KG  GI G +G          +G+ G  G IGIQG  G  G+ G KG +G+ G PG  G PG +G  G +G  G  GE
Sbjct: 1939 KGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGE 2053          

HSP 11 Score: 56.225 bits (134), Expect = 3.339e-7
Identity = 44/130 (33.85%), Postives = 66/130 (50.77%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTR---GEVGMKGVKGEYGIQGPRGPEGIAGVKGDI------------------GIQGETGPRGKDGN-------KGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            +G +G++G++G KG+ G  G+ G R   GE+G+ G+ G+ G  G  G +GI G KG++                  GI+G  G +G  G        +GD G  G PG  GN G+KG +G +G  G  G+
Sbjct: 1858 RGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQSNVNRGPQGDPGLAGIPGENGNIGVKGEKGLMGISGEKGD 1987          

HSP 12 Score: 55.4546 bits (132), Expect = 5.483e-7
Identity = 47/108 (43.52%), Postives = 60/108 (55.56%), Query Frame = 2
Query: 1829 KGARGIRGSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEG---IAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPG---IKGSRGPVGNQGRDGE 2134
            KG  GI G+ G  G  G  G+ G +G  G KG KGE G QG RG  G   + G+ G IG  G+ G +G  G KG+ G  GP G +G PG   IKG +G  G++G  G+
Sbjct: 1840 KGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPKGNSGTPGKMAIKGIKGTKGSKGIQGQ 1947          

HSP 13 Score: 52.7582 bits (125), Expect = 3.543e-6
Identity = 44/112 (39.29%), Postives = 60/112 (53.57%), Query Frame = 2
Query: 1826 YKGARGIRGSS--GAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGY------AGNPGIKGSRGPVGNQGRDGEK 2137
            + G  GI+G +  G  G  G  G  G +GE G  G+ G  G+ G  G  G  GVKG+ G +GE G  G+ GN+G+ G +G PG       +G PG +G +G  GN G+ G K
Sbjct: 1810 FPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKKGVKGNNGQKGEKGEIGRQGNRGEMGEIGLPGILGKIGTSGKPGKQGIKGFKGNVGKSGPK 1921          

HSP 14 Score: 51.9878 bits (123), Expect = 6.434e-6
Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = 2
Query: 1850 GSSGAKGDHGPSGQVGTRGEVGMKGVKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGK------DGNKGDEGSVGPPGYAGNP---GIKGSRGPVGNQGRDG 2131
            G+ G KG+ G  G  G +G++G  G  G+ G+ G  G  GI G+KG+IG  GE G +G       +G  GD+G +G  G  G P   G++G+ GP G++G  G
Sbjct: 1968 GNIGVKGEKGLMGISGEKGDIGKSGKLGKDGLIGEPGKIGIQGLKGEIGQPGEKGTKGAKGLPGIEGEPGDQGEIGAMGLIGLPGESGLRGTPGPEGDEGLHG 2070          

HSP 15 Score: 51.6026 bits (122), Expect = 7.422e-6
Identity = 45/106 (42.45%), Postives = 54/106 (50.94%), Query Frame = 2
Query: 1832 GARGIRGSSGAKGDHGPSGQVGTRGEVGMKG-----VKGEYGIQGPRGPEGIAGVKGDIGIQGETGPRGKDGNKGDEGSVGPPGYAGNPGIKGSRGPVGNQGRDGE 2134
            GA+G  G  G  G  G  G +G RG  G  G     VKGE G+QG +G  G  G KG+ GI G  G  G  GN+G +      G  GN G KG +G +G QG  GE
Sbjct: 1785 GAKGDIGEKGLPGKKGEKGDIGFRGFPGENGIKGVAVKGEAGVQGSQGMPGYKGEKGNTGIGGNPGLLGFAGNRGKK------GVKGNNGQKGEKGEIGRQGNRGE 1884          
The following BLAST results are available for this feature:
BLAST of Collagen type IV alpha 2 chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
COL24A11.799e-3331.93collagen type XXIV alpha 1 chain [Source:HGNC Symb... [more]
COL27A11.425e-2631.61collagen type XXVII alpha 1 chain [Source:HGNC Sym... [more]
COL11A18.275e-2525.86collagen type XI alpha 1 chain [Source:HGNC Symbol... [more]
COL11A12.115e-2425.86collagen type XI alpha 1 chain [Source:HGNC Symbol... [more]
COL11A12.288e-2425.86collagen type XI alpha 1 chain [Source:HGNC Symbol... [more]
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BLAST of Collagen type IV alpha 2 chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
col-1357.147e-2739.21COLlagen [Source:UniProtKB/TrEMBL;Acc:G3MU58][more]
col-1359.852e-2135.10COLlagen [Source:UniProtKB/TrEMBL;Acc:G3MU58][more]
emb-93.424e-1132.14Collagen alpha-1(IV) chain [Source:UniProtKB/Swis... [more]
dpy-171.739e-934.33DumPY: shorter than wild-type [Source:UniProtKB/T... [more]
let-27.581e-937.62Collagen alpha-2(IV) chain [Source:UniProtKB/Swis... [more]
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BLAST of Collagen type IV alpha 2 chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Col4a12.096e-1637.18gene:FBgn0000299 transcript:FBtr0079003[more]
Col4a12.096e-1637.18gene:FBgn0000299 transcript:FBtr0079002[more]
Col4a12.096e-1637.18gene:FBgn0000299 transcript:FBtr0079001[more]
vkg8.747e-1634.98gene:FBgn0016075 transcript:FBtr0333541[more]
vkg8.747e-1634.98gene:FBgn0016075 transcript:FBtr0079036[more]
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BLAST of Collagen type IV alpha 2 chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
col27a1b1.091e-3929.81collagen, type XXVII, alpha 1b [Source:NCBI gene;A... [more]
si:dkey-21p1.31.178e-3732.02si:dkey-21p1.3 [Source:ZFIN;Acc:ZDB-GENE-081105-16... [more]
col1a21.317e-2932.45collagen, type I, alpha 2 [Source:ZFIN;Acc:ZDB-GEN... [more]
col11a1a1.979e-2925.68collagen, type XI, alpha 1a [Source:ZFIN;Acc:ZDB-G... [more]
col11a1a2.040e-2925.68collagen, type XI, alpha 1a [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of Collagen type IV alpha 2 chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000017769.11.593e-3731.85collagen, type XXVII, alpha 1 [Source:NCBI gene;Ac... [more]
ENSXETT00000032329.11.606e-3731.85collagen, type XXVII, alpha 1 [Source:NCBI gene;Ac... [more]
COL5A16.949e-3529.33collagen type V alpha 1 chain [Source:HGNC Symbol;... [more]
COL5A18.428e-3529.33collagen type V alpha 1 chain [Source:HGNC Symbol;... [more]
COL5A11.060e-3428.24collagen type V alpha 1 chain [Source:HGNC Symbol;... [more]
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BLAST of Collagen type IV alpha 2 chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5