Epiphycan
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003556 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Epiphycan vs. Ensembl Human
Match: LGR4 (leucine rich repeat containing G protein-coupled receptor 4 [Source:HGNC Symbol;Acc:HGNC:13299]) HSP 1 Score: 105.531 bits (262), Expect = 3.139e-22 Identity = 113/402 (28.11%), Postives = 186/402 (46.27%), Query Frame = 2
Query: 185 CPIPCKC-THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMT---KLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF--------VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-NLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPA-SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIES 1345
C PC C VDC + L VP+G+S L L+ N + + + L LK L L +N++K + A GLS L++L L+ N ++ + PE +GL++L H++L +N ++PV +++ L L NKI S++ D +F + L L L N+I+ ++Q F ++L L + +L ++ F S+I + +GN LL + + F G + F L S LH L + SM++ N G +L+ L L KIS P+ L L ++ NN+ + P +L+ + LQ N I QI++ F+ L +L+ L L +N I I + +F L +T+LD+ FN LT +
Sbjct: 29 CAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSV-PEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKI-SSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNL--------------------SDLHSLVIRGASMVQQFPN--LTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPT 406 HSP 2 Score: 80.1073 bits (196), Expect = 1.667e-14 Identity = 82/288 (28.47%), Postives = 125/288 (43.40%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP-HRLP--ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQ------------QNPVLLTIA----PNSFV-------LSNLESIELQGNRI--KFDATAG-----------------------------------------PIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMN 1621
+N+ LK++ ++A RGL LQ L L I+ P+ F + +L HL+++DN+L P H L +L+ L L N I I F +L++L LHL N I S+ + FD L +L +LDL +N L I +P L L NP+L TI P SFV LS+L S+ ++G + +F G P F+ L +SL+ NQI ++ E F G ++++++DLS N
Sbjct: 89 QNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAI-KALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRN 375 HSP 3 Score: 57.3806 bits (137), Expect = 1.509e-7 Identity = 50/177 (28.25%), Postives = 86/177 (48.59%), Query Frame = 2
Query: 1109 NDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWP 1639
+ L P L A + L L N++ I L LK L L+ N + ++ + + L++L SL L N +T + F + +L L L+ + + + P S L L+++ L N+I + F NL LV L L +N+I L+++ F G N++ +DL+ N+L +P
Sbjct: 44 SGKGLTAVPEGLSAFTQALQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLS-NLPTLQALTLALNKIS--SIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFP 217 HSP 4 Score: 57.3806 bits (137), Expect = 1.789e-7 Identity = 51/188 (27.13%), Postives = 91/188 (48.40%), Query Frame = 2
Query: 1076 SAMAKLTHLFMNDNNLINCPH---RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
S + +L L + +N L P R ++L+ L L +N+I + +D F+ L L+ L L NS+ + + +L +L +L L N+++ I F + L+ L+L N + +++ + F L NLE+++L N + A LP L L N I+ + + F G+ ++ I L N L
Sbjct: 78 SGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHL--HNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQA---IKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPL 260
BLAST of Epiphycan vs. Ensembl Human
Match: LGR4 (leucine rich repeat containing G protein-coupled receptor 4 [Source:HGNC Symbol;Acc:HGNC:13299]) HSP 1 Score: 104.375 bits (259), Expect = 7.178e-22 Identity = 116/425 (27.29%), Postives = 188/425 (44.24%), Query Frame = 2
Query: 185 CPIPCKC-THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-----------------------PETFNKGLIKLLHVYLGNNEFEKIPVLKATHMT---KLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF--------VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-NLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPA-SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIES 1345
C PC C VDC + L VP+G+S LD+S N I L ++F+ L+ L+L+ N++ I+ A GL L+ L L+NN L + PE +GL++L H++L +N ++PV +++ L L NKI S++ D +F + L L L N+I+ ++Q F ++L L + +L ++ F S+I + +GN LL + + F G + F L S LH L + SM++ N G +L+ L L KIS P+ L L ++ NN+ + P +L+ + LQ N I QI++ F+ L +L+ L L +N I I + +F L +T+LD+ FN LT +
Sbjct: 29 CAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKI-SSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNL--------------------SDLHSLVIRGASMVQQFPN--LTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPT 430 HSP 2 Score: 80.1073 bits (196), Expect = 1.988e-14 Identity = 82/288 (28.47%), Postives = 125/288 (43.40%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP-HRLP--ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQ------------QNPVLLTIA----PNSFV-------LSNLESIELQGNRI--KFDATAG-----------------------------------------PIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMN 1621
+N+ LK++ ++A RGL LQ L L I+ P+ F + +L HL+++DN+L P H L +L+ L L N I I F +L++L LHL N I S+ + FD L +L +LDL +N L I +P L L NP+L TI P SFV LS+L S+ ++G + +F G P F+ L +SL+ NQI ++ E F G ++++++DLS N
Sbjct: 113 QNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAI-KALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRN 399 HSP 3 Score: 63.929 bits (154), Expect = 1.952e-9 Identity = 71/260 (27.31%), Postives = 125/260 (48.08%), Query Frame = 2
Query: 866 GFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPH---RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
G + +D GLT++ + L F + LD+S N++ + + DAF+ L+ L LA +S P S + +L L + +N L P R ++L+ L L +N+I + +D F+ L L+ L L NS+ + + +L +L +L L N+++ I F + L+ L+L N + +++ + F L NLE+++L N + A LP L L N I+ + + F G+ ++ I L N L
Sbjct: 37 GDRRVDCSGKGLTAVP-EGLSAFTQA----LDISMNNITQ-LPEDAFKNFPFLEELQLAGNDLSFIHPK-ALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHL--HNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQA---IKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPL 284 HSP 4 Score: 55.0694 bits (131), Expect = 9.764e-7 Identity = 55/201 (27.36%), Postives = 94/201 (46.77%), Query Frame = 2
Query: 1109 NDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFK---------------------SLTNLKELH---LKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWP 1639
+ L P L A + L + NNI Q+ +D FK +L+ LKEL L+ N + ++ + + L++L SL L N +T + F + +L L L+ + + + P S L L+++ L N+I + F NL LV L L +N+I L+++ F G N++ +DL+ N+L +P
Sbjct: 44 SGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLS-NLPTLQALTLALNKIS--SIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFP 241
BLAST of Epiphycan vs. Ensembl Human
Match: IGFALS (insulin like growth factor binding protein acid labile subunit [Source:HGNC Symbol;Acc:HGNC:5468]) HSP 1 Score: 99.3673 bits (246), Expect = 1.096e-20 Identity = 132/494 (26.72%), Postives = 205/494 (41.50%), Query Frame = 2
Query: 185 CPIPCKCTHH------SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDF-------------------LKPFFDSKLHHLD---LSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLF---MNDNNLINCPH---RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQI 1555
CP C C++ SV C + L +P G+ L L N + S+ +FQ L +L L L ++ + A +GL NL +LHLE N L L TF L + L NN ++ + LNL N + + + D +F L+ L+L N + + F +L L L N+L I A FV + L L L+ N I A F G ++LDL + + D L+P LH L+ L N + + + +F GL L++L L ++ +++ A LT++ ++ N L N P R L LHL+ + + +IR F L+ L+ L LK N ++ IE S LA L LDL N+LT + +F + KL L L + L P + + L + + +A + L +L LSLR+N +
Sbjct: 41 CPAACVCSYDDDADELSVFCSSRNLTRLPDGVPGGTQALWLDGNNLSSVPPAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAH-TPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSL-AVLPDAAFRGLGSLRELVLAGNRLAYLQPALF--SGLAELRELDLSRNALRAIKANVFVQLPR-LQKLYLDRN-----LIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRI-RQLAERSFEGLGQLEVLTLDHNQLQ--EVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQLTHLPHRLFQGLGKLEYLLLSRNR---LAELPADALGPLQRAFWLDVSHNRLEALPNSLLAPLGRLRYLSLRNNSL 518 HSP 2 Score: 74.7146 bits (182), Expect = 5.378e-13 Identity = 63/214 (29.44%), Postives = 95/214 (44.39%), Query Frame = 2
Query: 1100 LFMNDNNLINCPHRLPA------SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKF----------------------DATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTF 1651
L+++ NNL + P PA SL FL+LQ + + L NL LHL++N + S+ +F +L SL L NRL+R+E G+F + L LNL + L + P++ L +L + L GNR+ + A +F LP+L L L N I + F G ++ +DLS N + G TF
Sbjct: 79 LWLDGNNLSSVP---PAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNS---LAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTF 286
BLAST of Epiphycan vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 100.523 bits (249), Expect = 1.307e-20 Identity = 158/643 (24.57%), Postives = 275/643 (42.77%), Query Frame = 2
Query: 176 VFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDS---NKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKS-----CGLTSISGDFLK--------PFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDL---GFNRLTRIES-GIFHRMPKLISLNLEQQNPVLLTIAP---------------------------------NSFVLSNLESIELQGNRIKFDATA----GPIFDNLPK-------LVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID-FNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQIL-CNIPNEKAGK 1894
V CP C C+++ VDCR + L ++P + +T + L N IK + +F L+R+ LS+N++ E+ +AF GL +L +L L N ++ L P++ +GL L + L N+ + V + LNL S NK+++ + +F +QT+ L +N F+ + K L N +E A P + + + +K + E + +G F DL C T++ K P + ++L L+ +E ++L++ F+ L L+ +N + KI+D + F + + + + N L N H++ SLK L L+SN I + D F L++++ L L N I ++ +FD L SL++L+L FN + G + R ++++ N Q P L P ++ V + + +++ I D T G F +PK L + L +N+I+ L+ FS + + LS N L P +TF L S + + L+ N +S + FN D + L ++++ N C C+ +QWL + K I C P E A K
Sbjct: 270 VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITEL-PKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQT-IAKGTFSPLRAIQTMHLAQNP--------FICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDC---FADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAEL-RLNNNEFTVLEAT--GIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRL-LSLHGNDISVVPEGAFN-------DLSALSHLAIGANPLYCDCN-MQWLSDWVKSEYKEPGIARCAGPGEMADK 887 HSP 2 Score: 79.337 bits (194), Expect = 4.223e-14 Identity = 62/185 (33.51%), Postives = 86/185 (46.49%), Query Frame = 2
Query: 140 LLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF 685
L+L I N V CP C C+ +VDC AL+ VP+ I + LDL+ N I ++ F L +L+ L+L N++ I AF L L L L N L L PE G KL + L N+ + IP A + L LD N+I S + D +F L+ L L N I ++ F
Sbjct: 15 LVLAILNKVAPQA--CPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQ-LFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQI-SCIEDGAFRALRDLEVLTLNNNNITRLSVASF 195 HSP 3 Score: 54.299 bits (129), Expect = 1.482e-6 Identity = 58/230 (25.22%), Postives = 88/230 (38.26%), Query Frame = 2
Query: 1133 PHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWL 1822
P +P + + L L NNI +I + DF L +L+ L L +N I +IE +F DL L L L N L +F KL L+L + NQI + F G+V+I+ + L N + F L + + LN N ++ +S+ S N++P KL+ L +N C CH + WL
Sbjct: 50 PRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSE---------------------------------------------------NQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEV-LTLNNNNITRLSV--ASFNHMP----KLRTFRLHSNNLYCDCH-LAWL 220 HSP 4 Score: 51.9878 bits (123), Expect = 7.768e-6 Identity = 37/119 (31.09%), Postives = 58/119 (48.74%), Query Frame = 2
Query: 167 CLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNS-FQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNE 520
C CP C+C +VDC +Q L +P+ I L L+ N L F+ L L+++ S+N++ +I E AF G S + + L +N L + + F KGL L + L +N
Sbjct: 492 CFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMF-KGLESLKTLMLRSNR 609
BLAST of Epiphycan vs. Ensembl Human
Match: SLIT2 (slit guidance ligand 2 [Source:HGNC Symbol;Acc:HGNC:11086]) HSP 1 Score: 100.523 bits (249), Expect = 1.473e-20 Identity = 158/643 (24.57%), Postives = 275/643 (42.77%), Query Frame = 2
Query: 176 VFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDS---NKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKS-----CGLTSISGDFLK--------PFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDL---GFNRLTRIES-GIFHRMPKLISLNLEQQNPVLLTIAP---------------------------------NSFVLSNLESIELQGNRIKFDATA----GPIFDNLPK-------LVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID-FNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQIL-CNIPNEKAGK 1894
V CP C C+++ VDCR + L ++P + +T + L N IK + +F L+R+ LS+N++ E+ +AF GL +L +L L N ++ L P++ +GL L + L N+ + V + LNL S NK+++ + +F +QT+ L +N F+ + K L N +E A P + + + +K + E + +G F DL C T++ K P + ++L L+ +E ++L++ F+ L L+ +N + KI+D + F + + + + N L N H++ SLK L L+SN I + D F L++++ L L N I ++ +FD L SL++L+L FN + G + R ++++ N Q P L P ++ V + + +++ I D T G F +PK L + L +N+I+ L+ FS + + LS N L P +TF L S + + L+ N +S + FN D + L ++++ N C C+ +QWL + K I C P E A K
Sbjct: 274 VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITEL-PKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQT-IAKGTFSPLRAIQTMHLAQNP--------FICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDC---FADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAEL-RLNNNEFTVLEAT--GIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRL-LSLHGNDISVVPEGAFN-------DLSALSHLAIGANPLYCDCN-MQWLSDWVKSEYKEPGIARCAGPGEMADK 891 HSP 2 Score: 78.9518 bits (193), Expect = 4.560e-14 Identity = 62/185 (33.51%), Postives = 86/185 (46.49%), Query Frame = 2
Query: 140 LLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF 685
L+L I N V CP C C+ +VDC AL+ VP+ I + LDL+ N I ++ F L +L+ L+L N++ I AF L L L L N L L PE G KL + L N+ + IP A + L LD N+I S + D +F L+ L L N I ++ F
Sbjct: 15 LVLAILNKVAPQA--CPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQ-LFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQI-SCIEDGAFRALRDLEVLTLNNNNITRLSVASF 195 HSP 3 Score: 54.299 bits (129), Expect = 1.534e-6 Identity = 58/230 (25.22%), Postives = 88/230 (38.26%), Query Frame = 2
Query: 1133 PHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWL 1822
P +P + + L L NNI +I + DF L +L+ L L +N I +IE +F DL L L L N L +F KL L+L + NQI + F G+V+I+ + L N + F L + + LN N ++ +S+ S N++P KL+ L +N C CH + WL
Sbjct: 50 PRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSE---------------------------------------------------NQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEV-LTLNNNNITRLSV--ASFNHMP----KLRTFRLHSNNLYCDCH-LAWL 220 HSP 4 Score: 51.9878 bits (123), Expect = 8.106e-6 Identity = 37/118 (31.36%), Postives = 58/118 (49.15%), Query Frame = 2
Query: 167 CLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNS-FQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNN 517
C CP C+C +VDC +Q L +P+ I L L+ N L F+ L L+++ S+N++ +I E AF G S + + L +N L + + F KGL L + L +N
Sbjct: 496 CFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMF-KGLESLKTLMLRSN 612
BLAST of Epiphycan vs. Ensembl Celegans
Match: lron-15 (ELRR (Extracellular Leucine-Rich Repeat) ONly [Source:UniProtKB/TrEMBL;Acc:Q18902]) HSP 1 Score: 91.2781 bits (225), Expect = 3.399e-18 Identity = 144/522 (27.59%), Postives = 239/522 (45.79%), Query Frame = 2
Query: 269 DVTILDLSFNLIKSLSNNSFQTL-INLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKG-LIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNT-----------------------GLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLK---FLHLQSNNIRQIRQDDF------KSLTNLKELHLKKNSILSIETN-SFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRI-KFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQL--IDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTIS 1714
D+ LD S N I++++ N+F+ L + L +L LSHN + I A L +L+ LHLENN + VL TF++ L L +YL NN+ IP L H+ + L R + K L T LQ++ L N I + T+ V ++ L N + + +F + + L++ N++ VE + F Q LDL + + LK F + L L L+EN M+ + +A R L NL LNL K++ D D+ L LF+ +N L + PH + + +K L + N I +++ + + ++ L+L N I ++ F+ + LT +D+ FNR+ I +F ++ L SL L+ L P+ F L L + L N+I K D + +LPKL +LSL NQ+ + EN+F S + +L ++L+ N + +++F L + +RL+ N + TI+
Sbjct: 119 DIQTLDFSNNQIQTVNVNAFRGLEMKLTQLDLSHNNLSVIPTWALTYLHSLQILHLENNRIDVLRSNTFDETQLNNLQFLYLDNNQLRIIPNLAFNHLRLVVLMLANNRITEIQKMSLPQTLKFLVLRNNLLTQIPYVALNDLKTLQSIDLEGNNITHLMDTNEVTFESEMKVILRN--NKVRRLDKHSFRSFR-KIRELDISYNQI---QTVEDS--SFETVGHMQSLDLSYNRIAYLPRGMLKNFAKT-LKTLKLAEN-MIHATP-EALRDLRNLTHLNLNGNKLNRIDGDVLKGCTDTLVELFIANNYLEHIPHGVLSGMKQLEHLDISKNKIMSLKKPTSLLSITKEETSTVRRLNLAGNRINNMSDYLIFEHMPLLTYVDVSFNRIRFISPRVFEKLKNLESLFLQNNQ---LAHFPSLFRLDKLRHLMLDNNQIQKIDNFS---LADLPKLQHLSLAGNQLDIITENMFGSSSSSELKSLNLAHNKIHSISSRSFSDL-DNLQQLRLSHNNIRTIT 622 HSP 2 Score: 83.1889 bits (204), Expect = 8.659e-16 Identity = 144/539 (26.72%), Postives = 246/539 (45.64%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMK-----------------EINEN-------AFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP--VLKAT-HMTKLNLDSNKIRSAVVDKSFLLNTGLQT-----LILTKNEIKEINQTDF-VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKL-----------LNSTIVE----QALTGFNGA--TGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLI----NCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISL---NLEQQNPVLLTIAPNSFVLSNLESIELQGNR-IKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID 1720
LD+S+N I+++ ++SF+T+ +++ L LS+N + ++ EN A L NL +L+L N L+ + + L+ +++ NN E IP VL + L++ NKI S S L T +T L L N I N +D+ + LT + + N + I+ F +KN L +L L+ N+L L +++ Q + F+ A Q L L L I+ + S+L L+L+ N + SI + +F LDNLQ L L+ I + FS + L +L ++ N +I + ++LPA L LHL NN+ +I +D F+S ++L+ L L N+ ++ + LDL N++ I+ R + +SL ++E+ N LL A + L SI++ N I D+ A F KL ++ L N I L + F ++ +D+S N ++ TF ++ + + +N NKLS I ++
Sbjct: 315 LDISYNQIQTVEDSSFETVGHMQSLDLSYNRIAYLPRGMLKNFAKTLKTLKLAENMIHATPEALRDLRNLTHLNLNGNKLNRIDGDVLKGCTDTLVELFIANNYLEHIPHGVLSGMKQLEHLDISKNKIMSLKKPTSLLSITKEETSTVRRLNLAGNRIN--NMSDYLIFEHMPLLTYVDVSFNRIRFISPRVFEKLKN-LESLFLQNNQLAHFPSLFRLDKLRHLMLDNNQIQKIDNFSLADLPKLQHLSLAGNQLDIITENMFGSSSSSELKSLNLAHNK-IHSISSRSFSDLDNLQQLRLSHNNIRTITSMTFSNLRNLRYLDLSHNRIIKILPSALYQLPA-LDVLHLDHNNLNEIDRDAFRSFSDLQSLKLSHNAFRRFSCEFLGSISQVHQLDLSSNQINEIDIFCIARGIRKLSLASNSVEKINRKLLQDA------TELTSIDISHNGIIDVDSDA---FCECRKLSHIKLSHNYIRNLWKGTFQYQESLHTLDISFNDILFLHQGTFGK--NNILQLHVNNNKLSRIPLE 837 HSP 3 Score: 67.781 bits (164), Expect = 4.649e-11 Identity = 120/464 (25.86%), Postives = 201/464 (43.32%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPET-FNKGLIKLLHVYLGN-NEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLN-TGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFD-SKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAK-----LTHLFMNDNNLINCP----HRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIK--FDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
L+L+ N I S+S+ SF L NL++L+LSHN ++ I F L NLR L L +N + + P + + +LH+ N NE ++ + + L L N R + FL + + + L L+ N+I EI+ + + L+L NS+E I KLL AT +D+ G+ + D F + KL H+ LS N ++++ F+ ++L L+ IS DI F + L +N+N L P ASL L L NNI+ + S NL L N + SIE +F++L SL LDL N +T F + IS ++ N L ++ F +++S+ + N+I + P L K+V L + N + +++ F ++++ +++S N +
Sbjct: 587 LNLAHNKIHSISSRSFSDLDNLQQLRLSHNNIRTITSMTFSNLRNLRYLDLSHNRIIKILPSALYQLPALDVLHLDHNNLNEIDRDAFRSFSDLQSLKLSHNAFRRFSCE--FLGSISQVHQLDLSSNQINEID----IFCIARGIRKLSLASNSVEKI------------------NRKLL------------QDATELTSIDISHNGIIDVDSD---AFCECRKLSHIKLSHN-YIRNLWKGTFQYQESLHTLD-----ISFNDILFLHQGTFGKNNILQLHVNNNKLSRIPLEALSSTMASLHLLDLAHNNIKIVDSSQLTSFGNLSILSFANNKVDSIEDGAFENLLSLKILDLSNNPVTSWSPTAFRDLSHSIS-SINMANTGLFSMP--KFSHRSIQSLNISCNKIYELSEKDLAP----LTKVVALDISHNNLKQISSMAFEPLIHLKQLNVSANPI 998 HSP 4 Score: 65.0846 bits (157), Expect = 3.345e-10 Identity = 103/411 (25.06%), Postives = 178/411 (43.31%), Query Frame = 2
Query: 731 NSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLS--ENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH---------------------------LQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPK-LISLNLEQQNPVLLTIAPNSFV-LSNLESIELQG---NRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLIS---SAIDIRLN-----------------MNKLSTISIDFNSTNYL-PIDFAKLKNIS---LENNQ 1789
N ++ + AF ++ L L+L N L S I ALT + Q L L++ + + + F +++L++L +N+ L+ I N AF L L +L LA +I++ + S L L + +N L P+ LK L L++N +R++ + F+S ++EL + N I ++E +SF+ + + SLDL +NR+ + G+ K L +L L + ++ P + L NL + L G NRI D G LV L + +N + + + SG ++ +D+S N ++ T L I+ ++ RLN M L+ + + FN ++ P F KLKN+ L+NNQ
Sbjct: 128 NQIQTVNVNAFRGLEMKLTQLDLSHNNL--SVIPTWALTYLHS---LQILHLENNRIDVLRSN---TFDETQLNNLQFLYLDNNQLRIIPNLAFNHL-RLVVLMLANNRITEIQ-KMSLPQTLKFLVLRNNLLTQIPYVALNDLKTLQSIDLEGNNITHLMDTNEVTFESEMKVILRNNKVRRLDKHSFRSFRKIRELDISYNQIQTVEDSSFETVGHMQSLDLSYNRIAYLPRGMLKNFAKTLKTLKLAEN---MIHATPEALRDLRNLTHLNLNGNKLNRIDGDVLKGCT----DTLVELFIANNYLEHIPHGVLSGMKQLEHLDISKNKIMSLKKPTSLLSITKEETSTVRRLNLAGNRINNMSDYLIFEHMPLLTYVDVSFNRIRFISPRVFEKLKNLESLFLQNNQ 521 HSP 5 Score: 53.5286 bits (127), Expect = 1.066e-6 Identity = 122/459 (26.58%), Postives = 205/459 (44.66%), Query Frame = 2
Query: 263 SIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPV------LKATHMTKLNLDSNKIRSAVVDKSFLLNTG-LQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQI---------RQDDFKS-LTNLKELHLKKNSILSIETNSFD------DLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLN 1567
+ ++T +D+S N I + +++F L +KLSHN ++ + + F +L L + ND+ LH TF K I LHV NN+ +IP+ + + H+ L+L N I+ +VD S L + G L L N++ I F NL + K+ L+ N + + + AF + +S+ ++ + L ++ F+ + Q L++ + +S L P +K+ LD+S N+ LK I + AF L +L+ LN++ I+ + + KL L ++D + RLP S F Q NN++ R S L+NL LH + I+ ++ D D L L + LT+IE G F + + + +E N SIE +RI FD G +++LSL DN++T N
Sbjct: 747 ATELTSIDISHNGIIDVDSDAFCECRKLSHIKLSHNYIRNLWKGTFQYQESLHTLDISFNDILFLHQGTFGKNNILQLHV--NNNKLSRIPLEALSSTMASLHL--LDLAHNNIK--IVDSSQLTSFGNLSILSFANNKVDSIEDGAFENLLSLKILDLSN--NPVTSWSPTAFRDLSHSISSINMANTGLF-------SMPKFSHRS-IQSLNISCNKIYELSEKDLAPL--TKVVALDISHNN-LKQISSMAFEPLIHLKQLNVSANPITHLTNEHIQQLYKLETLHVSDMPFL---LRLPDSHAF--GQMNNLKHFFLYNIPDMARPYQISSILSNLPPLH---TIYVDIKESALDRQFYTADTRLLRHLVVAGRNLTKIEVGAFATI-RGFRVRIEIHN----------------SSIEEFPSRI-FDTLTG------ISILSLSLTDNKLTTFN 1154
BLAST of Epiphycan vs. Ensembl Celegans
Match: slt-1 (Slit homolog 1 protein [Source:UniProtKB/Swiss-Prot;Acc:G5EFX6]) HSP 1 Score: 83.5741 bits (205), Expect = 7.019e-16 Identity = 152/628 (24.20%), Postives = 242/628 (38.54%), Query Frame = 2
Query: 155 YNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFN-------KGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNE---------IKEIN-QTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS-DPDIRFSAMAKLTHLFMNDNNLINCPHRLP-------ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILS----------IETNSFD--DLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDAT----------AGPIFD--NLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLP----IDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQILCNIPN 1879
Y C CP+PC CT +VDCR L VP + T + L N I S+ ++SF+ L NL RL LS N + EI AF+GL NL L L N+++ L +TF L + F+ +P L + L+L N I+S + + +F T L TL L KN + +IN Q + L+ + F K S + + + +I + G T +D GL +I P F ++L LS N++ N L+NL++L+L+ I+ D F ++KL L +NDN L H ++L+ L L NNI+ F T ++E+ + N +L + +NS D+ N + F P+ + P + SN +++ +RI FD T P D L L L L N++ L N FS + + +S N L + PL + +N L +S+ N ++LP + + +I++ +N C C+ + + + C PN
Sbjct: 250 YKKTCETAEICPLPCTCTGTTVDCRDSGLTYVPTNLPPSTTEIRLEQNQISSIPSHSFKNLKNLTRLDLSKNIITEIQPKAFLGLHNLHTLVLYGNNITDLKSDTFEGLGSLQLLLLNANQLTCIRRGTFDHVPKL-----SMLSLYDNDIKS-ISEVTFQNLTSLSTLHLAKNPLICDCNLQWLAQINLQKNIETSGARCEQPKRLRKKKFATLPPNKF-KCKGSESFVSMYADSCFIDSICPTQCDCY-GTT----VDCNKRGLNTIPTSI--PRFATQLL---LSGNNISTVDLNSNIHVLENLEVLDLSNNHITFINDKSFEKLSKLRELRLNDNKL----HHFSSMVLDEQSNLEILDLSGNNIQCFSSIFFNKATRIREIKVIGNDLLCDCRILPLMSWLRSNSSHSIDIPPCQQFQYSDNESDKQRCAAF---PEETCSDDSNLCPPKCSCLDRVVRCSN-KNLTSFPSRIPFDTTELYLDANYINEIPAHDLNRLYSLTKLDLSHNRLISLENNTFSNLTRLSTLIISYNKL-----RCLQPLAFNG------LNALRILSLHGNDISFLPQSAFSNLTSITHIAVGSNSLYCDCNMAWFSKWIKSKFIEAGIARCEYPN 841 HSP 2 Score: 67.781 bits (164), Expect = 4.499e-11 Identity = 127/538 (23.61%), Postives = 200/538 (37.17%), Query Frame = 2
Query: 185 CPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIR-------SAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAE----AFVPVKNSLINLELEGNKLLNS----------TIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIR-FSAMAKLTHLFMNDNNLIN-----------------------CPHR-----LPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLES-IELQGNRIKFDATAGPIFDNLPKLVNLSLRD-----NQITKLNENLFSGSVNIQLIDLSMN 1621
CP C C +V C Q L +VP+ I D LDL N I + N F +L+NLK L+L N++ I+ +F L L+ L L N + L F L KL H+ L N+ + + + LNLD N I S+ V L G + + Q D N L LE + P+ ++E+ +K + T + +D + GLT + + + +L E + + SI + +F+ L NL L+L+K I++ + F + L L + NN+ + C R +P L L L N+I+ I + F++LT+L LHL KN ++ + N L L ++L N T SG PK + + T+ PN F ES + + + D+ D V+ + R I + L NI +DL+ N
Sbjct: 17 CPAECVCVDRTVSCVGQQLTEVPQNIPNDTIRLDLQDNEITKIGPNDFSSLMNLKALQLMDNQIVTIHNQSFSSLVFLQKLRLSRNRIRHLPDNVFQNNL-KLTHLDLSENDITVVSDAQLQGPEFLEVLNLDKNHIFCLENNVISSWVSLEVLTLNGNRLTTFEEPSNARFRQLDLFNNPWNCDCRLRWMRKWLEKAEGQNKTVCATPLNLQGSSIEILQDKFMTCSGNRKRRYKKTCETAEICPLPCTCTGTTVDCRDSGLTYVPTNLPPSTTEIRL------EQNQISSIPSHSFKNLKNLTRLDLSKNIITEIQPKAFLGLHNLHTLVLYGNNITDLKSDTFEGLGSLQLLLLNANQLTCIRRGTFDHVP-KLSMLSLYDNDIKSISEVTFQNLTSLSTLHLAKNPLI-CDCN----LQWLAQINLQKNIET---SGARCEQPKRL------RKKKFATLPPNKFKCKGSESFVSMYADSCFIDSICPTQCDCYGTTVDCNKRGLNTIPTSIPRFATQLLLSGNNISTVDLNSN 532 HSP 3 Score: 66.2402 bits (160), Expect = 1.601e-10 Identity = 65/248 (26.21%), Postives = 102/248 (41.13%), Query Frame = 2
Query: 1133 PHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQILCNIP 1876
P +P L LQ N I +I +DF SL NLK L L N I++I SF L L L L NR+ + +F KL L+L + + +++ A +LQ GP F L L+L N I L N+ S V++++ + LN N+L+T N A+ + + L NN WNC C +++W+ + + +++ +C P
Sbjct: 39 PQNIPNDTIRLDLQDNEITKIGPNDFSSLMNLKALQLMDNQIVTIHNQSFSSLVFLQKLRLSRNRIRHLPDNVFQNNLKLTHLDLSENDITVVSDA------------QLQ----------GPEF-----LEVLNLDKNHIFCLENNVISSWVSLEV-------------------------LTLNGNRLTTFEEPSN---------ARFRQLDLFNNPWNCDC-RLRWMRKWLEKAEGQNKTVCATP 224
BLAST of Epiphycan vs. Ensembl Celegans
Match: sma-10 (Leucine-rich repeats and immunoglobulin-like domains protein sma-10 [Source:UniProtKB/Swiss-Prot;Acc:Q965M2]) HSP 1 Score: 78.5666 bits (192), Expect = 1.955e-14 Identity = 137/607 (22.57%), Postives = 252/607 (41.52%), Query Frame = 2
Query: 128 FTFCLLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDN--LPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHF-RKLILNRDQIL--CNIPNEKAGKNLFLVMVNCSDV 1930
+ F +V ++ VC+ + C C + VDC L ++P I + IL LS N I+S+ + + L+ L +S+N ++ I+ F L NL+ L++ N +L + G++E H+ KL+L SN I S V + ++++ L++N I + + + L L NS+ +I + F N+L+ L+L N + T + Q F F L+ KL LDL+ N M++ + AF L +LQ ++LA+ + ++D C LK L+L +N ++ + + LT+L+ L L N I S +S+ L L L NR+ + SG F + +L L L + + ++ + V +S+L ++L N + G + N +P L +L +NQ+ + + F ++ +DL+ N + + F PL +LK + + ++ C C QI WL + +L L++ I+ C+ P A +L++V ++ +++
Sbjct: 12 YDFLPKVVTFDTVCIGL------CHCVGNVVDCSSLDLSEIPTTIPNNTRILLLSDNEIESIDKSRLKGFYFLQTLDISNNIIRHIDFEFFYNLPNLKILNIRKN---------------RLARIPRGSHEL--------GHLEKLDLRSNLI-STVTSEELSYLAAVRSVDLSRNLISYLPKP--TTSAKVNIEKLDLASNSITDIGTDHFSSF-NTLVTLKLARNHI---TTLNQ----------FSFSRLR------------------KLESLDLTRN-MIREVRFLAFNQLPSLQNVSLARNDVYR---------------LDDGMFYAC-----EGLKHLNLSTNRVQAVTEGWMFGLTSLEVLDLSYNQIQSFHISSWSHTPKLKWLSLHSNRIQSLPSGSFRVLRQLEELILSANS--IDSLHKFALVGMSSLHKLDLSSNTLAVCVEDGAVLYNTSMPFLRSLRFTNNQLRVIPKRAFERFPALEELDLTDNPIATIHPEAFEPL--------------------------------ELKRLVMNSSSILCDC-QISWLASWIYRLKLDKSSIIAKCSYPPPLA--DLYVVAIDTANL 496
BLAST of Epiphycan vs. Ensembl Celegans
Match: sma-10 (Leucine-rich repeats and immunoglobulin-like domains protein sma-10 [Source:UniProtKB/Swiss-Prot;Acc:Q965M2]) HSP 1 Score: 78.5666 bits (192), Expect = 1.955e-14 Identity = 137/607 (22.57%), Postives = 252/607 (41.52%), Query Frame = 2
Query: 128 FTFCLLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDN--LPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHF-RKLILNRDQIL--CNIPNEKAGKNLFLVMVNCSDV 1930
+ F +V ++ VC+ + C C + VDC L ++P I + IL LS N I+S+ + + L+ L +S+N ++ I+ F L NL+ L++ N +L + G++E H+ KL+L SN I S V + ++++ L++N I + + + L L NS+ +I + F N+L+ L+L N + T + Q F F L+ KL LDL+ N M++ + AF L +LQ ++LA+ + ++D C LK L+L +N ++ + + LT+L+ L L N I S +S+ L L L NR+ + SG F + +L L L + + ++ + V +S+L ++L N + G + N +P L +L +NQ+ + + F ++ +DL+ N + + F PL +LK + + ++ C C QI WL + +L L++ I+ C+ P A +L++V ++ +++
Sbjct: 12 YDFLPKVVTFDTVCIGL------CHCVGNVVDCSSLDLSEIPTTIPNNTRILLLSDNEIESIDKSRLKGFYFLQTLDISNNIIRHIDFEFFYNLPNLKILNIRKN---------------RLARIPRGSHEL--------GHLEKLDLRSNLI-STVTSEELSYLAAVRSVDLSRNLISYLPKP--TTSAKVNIEKLDLASNSITDIGTDHFSSF-NTLVTLKLARNHI---TTLNQ----------FSFSRLR------------------KLESLDLTRN-MIREVRFLAFNQLPSLQNVSLARNDVYR---------------LDDGMFYAC-----EGLKHLNLSTNRVQAVTEGWMFGLTSLEVLDLSYNQIQSFHISSWSHTPKLKWLSLHSNRIQSLPSGSFRVLRQLEELILSANS--IDSLHKFALVGMSSLHKLDLSSNTLAVCVEDGAVLYNTSMPFLRSLRFTNNQLRVIPKRAFERFPALEELDLTDNPIATIHPEAFEPL--------------------------------ELKRLVMNSSSILCDC-QISWLASWIYRLKLDKSSIIAKCSYPPPLA--DLYVVAIDTANL 496
BLAST of Epiphycan vs. Ensembl Celegans
Match: sma-10 (Leucine-rich repeats and immunoglobulin-like domains protein sma-10 [Source:UniProtKB/Swiss-Prot;Acc:Q965M2]) HSP 1 Score: 77.411 bits (189), Expect = 4.640e-14 Identity = 137/607 (22.57%), Postives = 252/607 (41.52%), Query Frame = 2
Query: 128 FTFCLLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDN--LPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHF-RKLILNRDQIL--CNIPNEKAGKNLFLVMVNCSDV 1930
+ F +V ++ VC+ + C C + VDC L ++P I + IL LS N I+S+ + + L+ L +S+N ++ I+ F L NL+ L++ N +L + G++E H+ KL+L SN I S V + ++++ L++N I + + + L L NS+ +I + F N+L+ L+L N + T + Q F F L+ KL LDL+ N M++ + AF L +LQ ++LA+ + ++D C LK L+L +N ++ + + LT+L+ L L N I S +S+ L L L NR+ + SG F + +L L L + + ++ + V +S+L ++L N + G + N +P L +L +NQ+ + + F ++ +DL+ N + + F PL +LK + + ++ C C QI WL + +L L++ I+ C+ P A +L++V ++ +++
Sbjct: 12 YDFLPKVVTFDTVCIGL------CHCVGNVVDCSSLDLSEIPTTIPNNTRILLLSDNEIESIDKSRLKGFYFLQTLDISNNIIRHIDFEFFYNLPNLKILNIRKN---------------RLARIPRGSHEL--------GHLEKLDLRSNLI-STVTSEELSYLAAVRSVDLSRNLISYLPKP--TTSAKVNIEKLDLASNSITDIGTDHFSSF-NTLVTLKLARNHI---TTLNQ----------FSFSRLR------------------KLESLDLTRN-MIREVRFLAFNQLPSLQNVSLARNDVYR---------------LDDGMFYAC-----EGLKHLNLSTNRVQAVTEGWMFGLTSLEVLDLSYNQIQSFHISSWSHTPKLKWLSLHSNRIQSLPSGSFRVLRQLEELILSANS--IDSLHKFALVGMSSLHKLDLSSNTLAVCVEDGAVLYNTSMPFLRSLRFTNNQLRVIPKRAFERFPALEELDLTDNPIATIHPEAFEPL--------------------------------ELKRLVMNSSSILCDC-QISWLASWIYRLKLDKSSIIAKCSYPPPLA--DLYVVAIDTANL 496
BLAST of Epiphycan vs. Ensembl Fly
Match: Toll-6 (gene:FBgn0036494 transcript:FBtr0075614) HSP 1 Score: 98.2117 bits (243), Expect = 3.211e-20 Identity = 145/527 (27.51%), Postives = 238/527 (45.16%), Query Frame = 2
Query: 266 IDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI-PVLKAT--HMTKLNLDSNKIRSAVVDK-SFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLI---NCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPI-DFAKLKNISLENNQWNCSCHQIQWL 1822
+D+ LD+S N L N F TL L+ L +++N + I + A GL NL+ L+L +N + L E F + + VYL NN + P L + + L+L N+I S +DK +F+ L L L+ N++ ++ F L L L+ N LENIAA+ F P+ N+L L L NKL ++LD + L + L LD N+ L + DAFR LQ LNL ++ + M L + + +N + + + +L L L N + I F+ L NL+ L+L +N I +E +F+ +S+ ++ L N L I +G+F MP L+ LN+ L V S L+ ++L NR+ + + D+ KL L + NQ+ ++ + S I+L+ L+ N + TF+ ++ + L N+++T +D S LP+ + L + N + C C+ I WL
Sbjct: 277 LDLEYLDVSHNDFVVLPANGFGTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIRLVLLNLSHNKLTKLEPEIF--SDLYTLQILNLRHNQLENIAADTFAPM-NNLHTLLLSHNKL-------------------KYLDAYA----------LNGLYVLSLLSLD---NNALIGVHPDAFRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFEMTSSIQAVRLDGNELNDI-NGLFSNMPSLLWLNISDNR---LESFDYGHVPSTLQWLDLHKNRLSSLSNRFGL-DSELKLQTLDVSFNQLQRIGPSSIPNS--IELLFLNDNLITTVDPDTFMH-KTNLTRVDLYANQITT--LDIKSLRILPVWEHRALPEFYIGGNPFTCDCN-IDWL 757 HSP 2 Score: 79.337 bits (194), Expect = 1.986e-14 Identity = 145/524 (27.67%), Postives = 216/524 (41.22%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLS-----------------------------NLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTK----LNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKI--SDPDIRFSAMAKLTHLFMNDNNLINCPHRLP--ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSV--NIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMN---KLSTISIDFN 1726
LDLS N I SL +N F TL L L +S N ++++NE F S +L L + +ND VL F G ++ L V NN + KA K LNL SNKI + + +Q + L N I +N F NL + L++ + I FV + L+ L L NKL T +E + F+ Q L+L+ L +I+ D P + LH L LS N LK +D A GL L +L+L + PD F + L L +N N L P L L+ + L N I + FK L NL L L N + +I ++F DL +L L+L NR+ +E G F + S+++++ L GN + +F N+P L+ L++ DN++ E+ G V +Q +DL N L S++ R ++ KL T+ + FN
Sbjct: 205 LDLSSNNIWSLPDNIFCTLSELSALNMSENRLQDVNELGFRDRSKEPTNGSTESTSTTESAKKSSSSSTSCSLDLEYLDVSHNDFVVLPANGF--GTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIR-LVLLNLSHNKL---TKLEPEI--FSDLYTLQILNLRHNQLENIAADTFAPM--NNLHTLLLSHNK-LKYLDAYALNGLYVLSLLSLDNNALIGVHPDA-FRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFE-------------------------MTSSIQAVRLDGNELN---DINGLFSNMPSLLWLNISDNRL----ESFDYGHVPSTLQWLDLHKNRL-------------SSLSNRFGLDSELKLQTLDVSFN 671
BLAST of Epiphycan vs. Ensembl Fly
Match: Toll-6 (gene:FBgn0036494 transcript:FBtr0306307) HSP 1 Score: 98.2117 bits (243), Expect = 3.211e-20 Identity = 145/527 (27.51%), Postives = 238/527 (45.16%), Query Frame = 2
Query: 266 IDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI-PVLKAT--HMTKLNLDSNKIRSAVVDK-SFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLI---NCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPI-DFAKLKNISLENNQWNCSCHQIQWL 1822
+D+ LD+S N L N F TL L+ L +++N + I + A GL NL+ L+L +N + L E F + + VYL NN + P L + + L+L N+I S +DK +F+ L L L+ N++ ++ F L L L+ N LENIAA+ F P+ N+L L L NKL ++LD + L + L LD N+ L + DAFR LQ LNL ++ + M L + + +N + + + +L L L N + I F+ L NL+ L+L +N I +E +F+ +S+ ++ L N L I +G+F MP L+ LN+ L V S L+ ++L NR+ + + D+ KL L + NQ+ ++ + S I+L+ L+ N + TF+ ++ + L N+++T +D S LP+ + L + N + C C+ I WL
Sbjct: 277 LDLEYLDVSHNDFVVLPANGFGTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIRLVLLNLSHNKLTKLEPEIF--SDLYTLQILNLRHNQLENIAADTFAPM-NNLHTLLLSHNKL-------------------KYLDAYA----------LNGLYVLSLLSLD---NNALIGVHPDAFRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFEMTSSIQAVRLDGNELNDI-NGLFSNMPSLLWLNISDNR---LESFDYGHVPSTLQWLDLHKNRLSSLSNRFGL-DSELKLQTLDVSFNQLQRIGPSSIPNS--IELLFLNDNLITTVDPDTFMH-KTNLTRVDLYANQITT--LDIKSLRILPVWEHRALPEFYIGGNPFTCDCN-IDWL 757 HSP 2 Score: 79.337 bits (194), Expect = 1.986e-14 Identity = 145/524 (27.67%), Postives = 216/524 (41.22%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLS-----------------------------NLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTK----LNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKI--SDPDIRFSAMAKLTHLFMNDNNLINCPHRLP--ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSV--NIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMN---KLSTISIDFN 1726
LDLS N I SL +N F TL L L +S N ++++NE F S +L L + +ND VL F G ++ L V NN + KA K LNL SNKI + + +Q + L N I +N F NL + L++ + I FV + L+ L L NKL T +E + F+ Q L+L+ L +I+ D P + LH L LS N LK +D A GL L +L+L + PD F + L L +N N L P L L+ + L N I + FK L NL L L N + +I ++F DL +L L+L NR+ +E G F + S+++++ L GN + +F N+P L+ L++ DN++ E+ G V +Q +DL N L S++ R ++ KL T+ + FN
Sbjct: 205 LDLSSNNIWSLPDNIFCTLSELSALNMSENRLQDVNELGFRDRSKEPTNGSTESTSTTESAKKSSSSSTSCSLDLEYLDVSHNDFVVLPANGF--GTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIR-LVLLNLSHNKL---TKLEPEI--FSDLYTLQILNLRHNQLENIAADTFAPM--NNLHTLLLSHNK-LKYLDAYALNGLYVLSLLSLDNNALIGVHPDA-FRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFE-------------------------MTSSIQAVRLDGNELN---DINGLFSNMPSLLWLNISDNRL----ESFDYGHVPSTLQWLDLHKNRL-------------SSLSNRFGLDSELKLQTLDVSFN 671
BLAST of Epiphycan vs. Ensembl Fly
Match: Toll-6 (gene:FBgn0036494 transcript:FBtr0306308) HSP 1 Score: 98.2117 bits (243), Expect = 3.211e-20 Identity = 145/527 (27.51%), Postives = 238/527 (45.16%), Query Frame = 2
Query: 266 IDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI-PVLKAT--HMTKLNLDSNKIRSAVVDK-SFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLI---NCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPI-DFAKLKNISLENNQWNCSCHQIQWL 1822
+D+ LD+S N L N F TL L+ L +++N + I + A GL NL+ L+L +N + L E F + + VYL NN + P L + + L+L N+I S +DK +F+ L L L+ N++ ++ F L L L+ N LENIAA+ F P+ N+L L L NKL ++LD + L + L LD N+ L + DAFR LQ LNL ++ + M L + + +N + + + +L L L N + I F+ L NL+ L+L +N I +E +F+ +S+ ++ L N L I +G+F MP L+ LN+ L V S L+ ++L NR+ + + D+ KL L + NQ+ ++ + S I+L+ L+ N + TF+ ++ + L N+++T +D S LP+ + L + N + C C+ I WL
Sbjct: 277 LDLEYLDVSHNDFVVLPANGFGTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIRLVLLNLSHNKLTKLEPEIF--SDLYTLQILNLRHNQLENIAADTFAPM-NNLHTLLLSHNKL-------------------KYLDAYA----------LNGLYVLSLLSLD---NNALIGVHPDAFRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFEMTSSIQAVRLDGNELNDI-NGLFSNMPSLLWLNISDNR---LESFDYGHVPSTLQWLDLHKNRLSSLSNRFGL-DSELKLQTLDVSFNQLQRIGPSSIPNS--IELLFLNDNLITTVDPDTFMH-KTNLTRVDLYANQITT--LDIKSLRILPVWEHRALPEFYIGGNPFTCDCN-IDWL 757 HSP 2 Score: 79.337 bits (194), Expect = 1.986e-14 Identity = 145/524 (27.67%), Postives = 216/524 (41.22%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLS-----------------------------NLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTK----LNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKI--SDPDIRFSAMAKLTHLFMNDNNLINCPHRLP--ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSV--NIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMN---KLSTISIDFN 1726
LDLS N I SL +N F TL L L +S N ++++NE F S +L L + +ND VL F G ++ L V NN + KA K LNL SNKI + + +Q + L N I +N F NL + L++ + I FV + L+ L L NKL T +E + F+ Q L+L+ L +I+ D P + LH L LS N LK +D A GL L +L+L + PD F + L L +N N L P L L+ + L N I + FK L NL L L N + +I ++F DL +L L+L NR+ +E G F + S+++++ L GN + +F N+P L+ L++ DN++ E+ G V +Q +DL N L S++ R ++ KL T+ + FN
Sbjct: 205 LDLSSNNIWSLPDNIFCTLSELSALNMSENRLQDVNELGFRDRSKEPTNGSTESTSTTESAKKSSSSSTSCSLDLEYLDVSHNDFVVLPANGF--GTLRRLRVLSVNNNGISMIADKALSGLKNLQILNLSSNKIVALPTELFAEQAKIIQEVYLQNNSISVLNPQLFSNLDQLQALDLSMNQITSTWIDKNTFVGLIR-LVLLNLSHNKL---TKLEPEI--FSDLYTLQILNLRHNQLENIAADTFAPM--NNLHTLLLSHNK-LKYLDAYALNGLYVLSLLSLDNNALIGVHPDA-FRNCSALQDLNLNGNQLKTVPLALRNMRHLRTVDLGENMITVMEDSAFKGLGNLYGLRLIGNYLENITMHTFRDLPNLQILNLARNRIAVVEPGAFE-------------------------MTSSIQAVRLDGNELN---DINGLFSNMPSLLWLNISDNRL----ESFDYGHVPSTLQWLDLHKNRL-------------SSLSNRFGLDSELKLQTLDVSFN 671
BLAST of Epiphycan vs. Ensembl Fly
Match: atk (gene:FBgn0036995 transcript:FBtr0331569) HSP 1 Score: 96.2857 bits (238), Expect = 1.113e-19 Identity = 115/454 (25.33%), Postives = 193/454 (42.51%), Query Frame = 2
Query: 218 VDCRHQALKDVPKGISIDVTI-----LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFD----------------------SKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKL--THLFMNDNNLINCPHRL--PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF--VLSNLESIELQGNRI 1477
+ + + +P S D+ + LDLS N I+ L + FQ + L+ L L+ NE++++ + +F+G+ L LHL+ N L E ++ +L + LNL SNK+ A+ D F N+ L+ L L++N I+ I+ T F + SL L+L GN LL+ ++ G + +DL ++ I D + + + KL +LDLS N +++++ A +GLD LQ LA K+ + D F + L +H N I P SL FL+L +N+ R + +S+ NL+ L L N + + T L L L + N++ RI+ F MP+L L++ +N L +I +F V N+ +++ GN I
Sbjct: 528 ISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLG----------------------EADERALLPLAELRNLNLQSNKL-EAITDNFFSNNSRLEQLDLSRNLIRSISPTAF---------------------------DTQRSLEYLDLSGNALLDISV------GLGNLNNLRDIDLSYNQISRIQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNE-IRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYI-SPESFHNANSLVFLNLSNNHFRNMENIGLRSMRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGSPFETMPRLRVLSM--RNNQLRSIKERTFRNVRGNIAILDVDGNPI 921 HSP 2 Score: 88.9669 bits (219), Expect = 2.058e-17 Identity = 121/459 (26.36%), Postives = 203/459 (44.23%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHR--LP-ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIEL---QGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
LD+S NL+ L + + L++L L+HN ++ I +A + + LR L + NN LS P F L L + L N+F ++ + + +L+L N + + SF N L+TL ++ NE+ +I+ + + + L + A ++ +K+ + L IVE+ + LK +TS+ K L LDLS+N ++ + F+G L++L+LA+ ++ D F + +L L + +N L R LP A L+ L+LQSN + I + F + + L++L L +N I SI +FD SL LDL N L I G+ + L+NL I+L Q +RI+ D G + N +V + L +N I +L + F +Q +DLS N +
Sbjct: 363 LDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPF-WNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGL-IELAPNSFRHNPLLETLNISSNELTKIHSSTLI------------HLERLFEVDA-SYNQLKSVIAGL---------PRIVER-------------ISLKGNQITSLPAAASKDLQLPNLRMLDLSQNR-IEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLGEADERALLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQRSLEYLDLSGNALLDISVGLGN--------------------------LNNLRDIDLSYNQISRIQSDVIGG--WRN---VVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNEI 752 HSP 3 Score: 87.0409 bits (214), Expect = 8.687e-17 Identity = 131/509 (25.74%), Postives = 231/509 (45.38%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPET-------------FNK------GLIKLL-HVYLGNNEFEKIPV-----LKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTG--FNGATGFQFLDLKSCGLTSISGDFLKPFFDSK--LHHLDLSENSMLK-SIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPH----RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQN-PVLLTIAPNSFVLSNLESIELQGNRIKFDAT----------------------AGPIFDNLPKLVNLSLRDNQITKLNENLFSG-SVNIQLIDLSMNSL 1627
LDL+ N + + L +L+RL LS N + E+ N+F L L++ +N+L+ +H T +N+ GL +++ + L N+ +P L+ ++ L+L N+I + F L+ L L +NE++++ T F+ ++ +L L N L A +P+ L NL L+ NKL +A+T F+ + + LDL + SIS FD++ L +LDLS N++L S+ L+NL+ ++L+ +IS D+ + + +++N ++ LP L++L L SN IR + K L L+E L N ++ ++ + F++L SL + +N+L I FH L+ LNL + + I S + NLE ++L N +K +T G F+ +P+L LS+R+NQ+ + E F NI ++D+ N +
Sbjct: 435 LDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQ-LPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQE--NQLGEADERALLPLA-ELRNLNLQSNKL-------EAITDNFFSNNSRLEQLDLSRNLIRSIS----PTAFDTQRSLEYLDLSGNALLDISV---GLGNLNNLRDIDLSYNQISRIQSDV-IGGWRNVVEIRLSNNLIVELQQGTFRNLP-KLQYLDLSSNEIRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLSNNHFRNMENIGLRS--MRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGSPFETMPRLRVLSMRNNQLRSIKERTFRNVRGNIAILDVDGNPI 921 HSP 4 Score: 80.1073 bits (196), Expect = 9.467e-15 Identity = 111/424 (26.18%), Postives = 191/424 (45.05%), Query Frame = 2
Query: 728 MNSLENIAAEAFVPVKNSLINLELEG-NKLLNSTIVE------QALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVL---SNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMN--SLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDF---AKLKNISLENNQW-NCSCHQIQWLLHFRKLILNRDQILCNIPNEKAGKNLFLVMVNCSDVILSRILS 1951
+N LEN E FV V+ L ++ E N ++N + + L F+G +L +++ L + + KL H+ ++ S L ++ F GL +L+ L+L+ ++ I A+++L +L SLK H Q +++ + + K L +LK+L L N I IE SF DL +L+ L L NR+T ++ G F R P+L ++ L QN ++ I P S + S +E++ + N I + D LP+L L + N +++L G ++ + L+ N LI A +P + ++R+ N LS+ LP+ F LK + L NQ+ + L R+L L+ + ++ PN N L +N S L++I S
Sbjct: 108 LNELENGLVEIFV-VEPQLRSIPAESLNGMINMLAITIQSEELKHLPDFSGLLSLTYLSVQTGALQELPSHLFRHL--PKLQHIHITGGSGLTRLEAGLFDGLISLKNLDLSHNGLNW--IHLRALSRLPNL---------------VSLKLSHNQISDVGMVGRI-VKDLEHLKKLRLDNNLITVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYL--QNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALR---ELRMRNNSLSS---------DLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFR-HNPLLETLNISSNELTKIHS 495 HSP 5 Score: 70.4774 bits (171), Expect = 9.939e-12 Identity = 74/286 (25.87%), Postives = 130/286 (45.45%), Query Frame = 2
Query: 1079 AMAKLTHLFMNDNNLINCPH---RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF------------------------------------------VLSNL----ESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQ 1789
A+ +L +L M+ N L P+ R +L+ LHL N++R I +D ++ L+EL ++ NS+ S F +L L LDL N+ R++S + +P L L+L + L+ +APNSF V++ L E I L+GN+I A LP L L L N+I +L + F G++ ++++ L+ N L +F+ + + + L N+L + + LP+ A+L+N++L++N+
Sbjct: 356 ALPRLRYLDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENG--LIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLEL-LHLQENQLG----EADERALLPL--AELRNLNLQSNK 632 HSP 6 Score: 70.0922 bits (170), Expect = 1.359e-11 Identity = 123/500 (24.60%), Postives = 214/500 (42.80%), Query Frame = 2
Query: 266 IDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVG-----LSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAA-EAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKI-SDPDIRFSAMAKLTHLFMNDNNLINCPHRLPA---SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTN 1735
I + LDLS N + + + L NL LKLSHN++ ++ VG L +L+ L L+NN ++V+ + F +P ++++L+L+ N+I + + +FL L+T+ L N I+ I+ + + + + + N + ++ A A + L L++ GN L S + AL G L L L+ N L+ I+ DA + L+ L + + SD + F + L L + N +L A SL+ L L N + ++ + F+ L+ L++ N + I +++ L L +D +N+L + +G+ R+ + ISL Q L A L NL ++L NRI+ G F +L LSL N++ +L + F G ++L+ L N L + LPL + ++ L NKL I+ +F S N
Sbjct: 208 ISLKNLDLSHNGLNWIHLRALSRLPNLVSLKLSHNQISDV---GMVGRIVKDLEHLKKLRLDNNLITVIE-----------------DGSFVDLP-----NLSELHLNDNRI-TELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNLL--SELPYGALRGH-----------------------------GTLEQLHLNHNH-LRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGL-PRIVERISLKGNQIT-SLPAAASKDLQLPNLRMLDLSQNRIEQLPRHG--FQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLGEADERALLPL-AELRNLNLQSNKLEAITDNFFSNN 644 HSP 7 Score: 67.3958 bits (163), Expect = 8.098e-11 Identity = 123/517 (23.79%), Postives = 211/517 (40.81%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETF---NKGLIKLLHVYLGNNEFEKIPVLKA-----THMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMND---NNLINCPHRLPASLKFLHLQSNNIRQIRQDDFK--SLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRI-KFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQ 1789
L L NLI + + SF L NL L L+ N + E+ AF+ L+ ++L+NN + +HPE+ + ++ +H+Y NNE + L+A + L++ N + S + + + L+ L L N ++ I + + M +L + ++ N+++ F G + LDL + L L LDLSEN +++ N +FR L+ LN++ +++ I S + L LF D N L + LP ++ + L+ N I + K L NL+ L L +N I + + F L L L N L +++ F + LE + LQ N++ + D A L +L NL+L+ N++ + +N FS + ++ +DLS N LI + T S + L+ N L IS+ + N L++I L NQ
Sbjct: 263 LRLDNNLITVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMY--NNEIGHVEALRALLDALPRLRYLDMSGNLL-SELPYGALRGHGTLEQLHLNHNHLRLIERDALMA------------MPALREL--------------------RMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGL--PSLRRLDLSENGLIELAPN-SFRHNPLLETLNISSNELTK--IHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSF-------------------------IGIQRLELLHLQENQLGEADERA---LLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRN-LIRSISPTAFDTQRSLEYLDLSGNALLDISVGLGNLN-------NLRDIDLSYNQ 703
BLAST of Epiphycan vs. Ensembl Fly
Match: atk (gene:FBgn0036995 transcript:FBtr0331570) HSP 1 Score: 96.2857 bits (238), Expect = 1.113e-19 Identity = 115/454 (25.33%), Postives = 193/454 (42.51%), Query Frame = 2
Query: 218 VDCRHQALKDVPKGISIDVTI-----LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFD----------------------SKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKL--THLFMNDNNLINCPHRL--PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF--VLSNLESIELQGNRI 1477
+ + + +P S D+ + LDLS N I+ L + FQ + L+ L L+ NE++++ + +F+G+ L LHL+ N L E ++ +L + LNL SNK+ A+ D F N+ L+ L L++N I+ I+ T F + SL L+L GN LL+ ++ G + +DL ++ I D + + + KL +LDLS N +++++ A +GLD LQ LA K+ + D F + L +H N I P SL FL+L +N+ R + +S+ NL+ L L N + + T L L L + N++ RI+ F MP+L L++ +N L +I +F V N+ +++ GN I
Sbjct: 528 ISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLG----------------------EADERALLPLAELRNLNLQSNKL-EAITDNFFSNNSRLEQLDLSRNLIRSISPTAF---------------------------DTQRSLEYLDLSGNALLDISV------GLGNLNNLRDIDLSYNQISRIQSDVIGGWRNVVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNE-IRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYI-SPESFHNANSLVFLNLSNNHFRNMENIGLRSMRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGSPFETMPRLRVLSM--RNNQLRSIKERTFRNVRGNIAILDVDGNPI 921 HSP 2 Score: 88.9669 bits (219), Expect = 2.058e-17 Identity = 121/459 (26.36%), Postives = 203/459 (44.23%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHR--LP-ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIEL---QGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
LD+S NL+ L + + L++L L+HN ++ I +A + + LR L + NN LS P F L L + L N+F ++ + + +L+L N + + SF N L+TL ++ NE+ +I+ + + + L + A ++ +K+ + L IVE+ + LK +TS+ K L LDLS+N ++ + F+G L++L+LA+ ++ D F + +L L + +N L R LP A L+ L+LQSN + I + F + + L++L L +N I SI +FD SL LDL N L I G+ + L+NL I+L Q +RI+ D G + N +V + L +N I +L + F +Q +DLS N +
Sbjct: 363 LDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPF-WNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGL-IELAPNSFRHNPLLETLNISSNELTKIHSSTLI------------HLERLFEVDA-SYNQLKSVIAGL---------PRIVER-------------ISLKGNQITSLPAAASKDLQLPNLRMLDLSQNR-IEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLGEADERALLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRNLIRSISPTAFDTQRSLEYLDLSGNALLDISVGLGN--------------------------LNNLRDIDLSYNQISRIQSDVIGG--WRN---VVEIRLSNNLIVELQQGTFRNLPKLQYLDLSSNEI 752 HSP 3 Score: 87.0409 bits (214), Expect = 8.687e-17 Identity = 131/509 (25.74%), Postives = 231/509 (45.38%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPET-------------FNK------GLIKLL-HVYLGNNEFEKIPV-----LKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTG--FNGATGFQFLDLKSCGLTSISGDFLKPFFDSK--LHHLDLSENSMLK-SIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPH----RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQN-PVLLTIAPNSFVLSNLESIELQGNRIKFDAT----------------------AGPIFDNLPKLVNLSLRDNQITKLNENLFSG-SVNIQLIDLSMNSL 1627
LDL+ N + + L +L+RL LS N + E+ N+F L L++ +N+L+ +H T +N+ GL +++ + L N+ +P L+ ++ L+L N+I + F L+ L L +NE++++ T F+ ++ +L L N L A +P+ L NL L+ NKL +A+T F+ + + LDL + SIS FD++ L +LDLS N++L S+ L+NL+ ++L+ +IS D+ + + +++N ++ LP L++L L SN IR + K L L+E L N ++ ++ + F++L SL + +N+L I FH L+ LNL + + I S + NLE ++L N +K +T G F+ +P+L LS+R+NQ+ + E F NI ++D+ N +
Sbjct: 435 LDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQ-LPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQE--NQLGEADERALLPLA-ELRNLNLQSNKL-------EAITDNFFSNNSRLEQLDLSRNLIRSIS----PTAFDTQRSLEYLDLSGNALLDISV---GLGNLNNLRDIDLSYNQISRIQSDV-IGGWRNVVEIRLSNNLIVELQQGTFRNLP-KLQYLDLSSNEIRNVEPGALKGLDELQEFVLADNKLVELKDHVFEELPSLLASHFQYNKLRYISPESFHNANSLVFLNLSNNHFRNMENIGLRS--MRNLEVLDLSTNGVKLVSTMPLKALNWLVELKMDNNQICRIQGSPFETMPRLRVLSMRNNQLRSIKERTFRNVRGNIAILDVDGNPI 921 HSP 4 Score: 80.1073 bits (196), Expect = 9.467e-15 Identity = 111/424 (26.18%), Postives = 191/424 (45.05%), Query Frame = 2
Query: 728 MNSLENIAAEAFVPVKNSLINLELEG-NKLLNSTIVE------QALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVL---SNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMN--SLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDF---AKLKNISLENNQW-NCSCHQIQWLLHFRKLILNRDQILCNIPNEKAGKNLFLVMVNCSDVILSRILS 1951
+N LEN E FV V+ L ++ E N ++N + + L F+G +L +++ L + + KL H+ ++ S L ++ F GL +L+ L+L+ ++ I A+++L +L SLK H Q +++ + + K L +LK+L L N I IE SF DL +L+ L L NR+T ++ G F R P+L ++ L QN ++ I P S + S +E++ + N I + D LP+L L + N +++L G ++ + L+ N LI A +P + ++R+ N LS+ LP+ F LK + L NQ+ + L R+L L+ + ++ PN N L +N S L++I S
Sbjct: 108 LNELENGLVEIFV-VEPQLRSIPAESLNGMINMLAITIQSEELKHLPDFSGLLSLTYLSVQTGALQELPSHLFRHL--PKLQHIHITGGSGLTRLEAGLFDGLISLKNLDLSHNGLNW--IHLRALSRLPNL---------------VSLKLSHNQISDVGMVGRI-VKDLEHLKKLRLDNNLITVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYL--QNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALR---ELRMRNNSLSS---------DLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFR-HNPLLETLNISSNELTKIHS 495 HSP 5 Score: 70.4774 bits (171), Expect = 9.939e-12 Identity = 74/286 (25.87%), Postives = 130/286 (45.45%), Query Frame = 2
Query: 1079 AMAKLTHLFMNDNNLINCPH---RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF------------------------------------------VLSNL----ESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQ 1789
A+ +L +L M+ N L P+ R +L+ LHL N++R I +D ++ L+EL ++ NS+ S F +L L LDL N+ R++S + +P L L+L + L+ +APNSF V++ L E I L+GN+I A LP L L L N+I +L + F G++ ++++ L+ N L +F+ + + + L N+L + + LP+ A+L+N++L++N+
Sbjct: 356 ALPRLRYLDMSGNLLSELPYGALRGHGTLEQLHLNHNHLRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENG--LIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSFIGIQRLEL-LHLQENQLG----EADERALLPL--AELRNLNLQSNK 632 HSP 6 Score: 70.0922 bits (170), Expect = 1.359e-11 Identity = 123/500 (24.60%), Postives = 214/500 (42.80%), Query Frame = 2
Query: 266 IDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVG-----LSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAA-EAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKI-SDPDIRFSAMAKLTHLFMNDNNLINCPHRLPA---SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTN 1735
I + LDLS N + + + L NL LKLSHN++ ++ VG L +L+ L L+NN ++V+ + F +P ++++L+L+ N+I + + +FL L+T+ L N I+ I+ + + + + + N + ++ A A + L L++ GN L S + AL G L L L+ N L+ I+ DA + L+ L + + SD + F + L L + N +L A SL+ L L N + ++ + F+ L+ L++ N + I +++ L L +D +N+L + +G+ R+ + ISL Q L A L NL ++L NRI+ G F +L LSL N++ +L + F G ++L+ L N L + LPL + ++ L NKL I+ +F S N
Sbjct: 208 ISLKNLDLSHNGLNWIHLRALSRLPNLVSLKLSHNQISDV---GMVGRIVKDLEHLKKLRLDNNLITVIE-----------------DGSFVDLP-----NLSELHLNDNRI-TELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMYNNEIGHVEALRALLDALPRLRYLDMSGNLL--SELPYGALRGH-----------------------------GTLEQLHLNHNH-LRLIERDALMAMPALRELRMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGLPSLRRLDLSENGLIELAPNSFRHNPLLETLNISSNELTKIHSSTLIHLERLFEVDASYNQLKSVIAGL-PRIVERISLKGNQIT-SLPAAASKDLQLPNLRMLDLSQNRIEQLPRHG--FQGAMELRVLSLAQNELRQLKDTSFIGIQRLELLHLQENQLGEADERALLPL-AELRNLNLQSNKLEAITDNFFSNN 644 HSP 7 Score: 67.3958 bits (163), Expect = 8.098e-11 Identity = 123/517 (23.79%), Postives = 211/517 (40.81%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETF---NKGLIKLLHVYLGNNEFEKIPVLKA-----THMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMND---NNLINCPHRLPASLKFLHLQSNNIRQIRQDDFK--SLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRI-KFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQ 1789
L L NLI + + SF L NL L L+ N + E+ AF+ L+ ++L+NN + +HPE+ + ++ +H+Y NNE + L+A + L++ N + S + + + L+ L L N ++ I + + M +L + ++ N+++ F G + LDL + L L LDLSEN +++ N +FR L+ LN++ +++ I S + L LF D N L + LP ++ + L+ N I + K L NL+ L L +N I + + F L L L N L +++ F + LE + LQ N++ + D A L +L NL+L+ N++ + +N FS + ++ +DLS N LI + T S + L+ N L IS+ + N L++I L NQ
Sbjct: 263 LRLDNNLITVIEDGSFVDLPNLSELHLNDNRITELQYGAFLRTPQLKTIYLQNNLIRRIHPESLLQASGSGVEAVHMY--NNEIGHVEALRALLDALPRLRYLDMSGNLL-SELPYGALRGHGTLEQLHLNHNHLRLIERDALMA------------MPALREL--------------------RMRNNSLSSDLPLPFWNLPGLKGLDLAQNQFARVDSQLLAGL--PSLRRLDLSENGLIELAPN-SFRHNPLLETLNISSNELTK--IHSSTLIHLERLFEVDASYNQLKSVIAGLPRIVERISLKGNQITSLPAAASKDLQLPNLRMLDLSQNRIEQLPRHGFQGAMELRVLSLAQNELRQLKDTSF-------------------------IGIQRLELLHLQENQLGEADERA---LLPLAELRNLNLQSNKLEAITDNFFSNNSRLEQLDLSRN-LIRSISPTAFDTQRSLEYLDLSGNALLDISVGLGNLN-------NLRDIDLSYNQ 703
BLAST of Epiphycan vs. Ensembl Zebrafish
Match: si:dkey-182i3.11 (si:dkey-182i3.11 [Source:ZFIN;Acc:ZDB-GENE-121214-258]) HSP 1 Score: 112.464 bits (280), Expect = 7.776e-25 Identity = 186/662 (28.10%), Postives = 291/662 (43.96%), Query Frame = 2
Query: 134 FCLLLVIYNLVCLYVFPCPIPCKC----------------------THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP--VLKAT-HMTKLNLDSNKIRSAVVDKSFLLN-TGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP----HRLPASLKFLHLQSNNI-----------RQIRQDDFKS--LT---------NLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI------------SIDFNSTNYLPID------FAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQILCNIPNEKAGKNL 1900
F LL+I +L+ V CPI C C T H R++ ++ +P ++ +LDL+ N+ N ++Q++ L L L N ++ + F GL NL+ L L NN + L P+ F GLI L + L N+ + VL+ +T L L N I +++ + N T L L L+KN++K + F T L L L +N L I N L +L L+ N + ++I + F+ + LDL GL SIS + S+L +LDLS N L+++ F L L+ LNL K++ P+ F + L L ++ NN+ P H L A LK L L +N+I RQ++Q D S LT NLKEL+L+ N I I SF +L L SL L N L+++ + + +L L L + + P F L NL ++L N++ F F++L L +L L N + L E++F+ N+ + L N L P++ F L+ ++ L+ N + I +D S ++ KLK I L+ N W+CS I L+ + N Q++ P +G+NL
Sbjct: 2 FWPLLIILDLIA-AVDACPISCSCVDVDIVTCWNTNLLEFPPLPNGTKHFYATRNK-IQALPNETFHELRVLDLTKNVFNLSLNTNWQSVRGLTHLHLGGNRLRALTPRQFEGLLNLQVLDLSNNAIKSL-PQMFLYGLINLQTLNLNINQILSLSYGVLEGPLALTDLQLRDNMID--MIEMNVFENCTYLAKLYLSKNKLKSVGNGSF--KGATGLNHLDLGLNGLAGIPTIVLQETSN-LTSLYLQKNDI--TSIPDNV---FSEILSLKHLDLSYNGLVSISNGSFRSL--SQLVYLDLSFNQ-LQTLTQHVFEDLGKLENLNLYHNKLTSLPNNMFKNLTMLKELQLDSNNISVIPPDLFHPLSA-LKDLQLDNNHISKLHSHTFKKLRQLKQLDISSNDLTKIPNHLFHKNLKELNLENNHISFISKFSFKNLHRLQSLKLSHNNLSKLYRELLTNLTRLRELLLNENQ---IETIPVGFFKGLENLRVLDLSNNKMHFILPDA--FNDLSALKDLDLSFNFLHNLPEDIFASLRNLTKLHLQNNKLRYLPSRLFSALVGLE-ELHLDRNYIQRIHPTQFEGLVKLHELDMKSNQLRSMEDGTLMPLRKLKRIHLDGNPWDCSTVVI---LYISQWFNNNTQLVKTTPMCSSGQNL 637
BLAST of Epiphycan vs. Ensembl Zebrafish
Match: igfals (insulin-like growth factor binding protein, acid labile subunit [Source:ZFIN;Acc:ZDB-GENE-061009-25]) HSP 1 Score: 105.531 bits (262), Expect = 9.289e-23 Identity = 140/504 (27.78%), Postives = 216/504 (42.86%), Query Frame = 2
Query: 182 PCPIPCKCTHH------SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSM-----------------------LKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLP-------ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPK----LISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKL 1564
PC C C +V C + VP I + L L NL +L +F+ L NL+ L L + + ++ +AF GL++L +LHLE N+L L F +G L + L NN+ +I +HM LN + S + + F GL+ LIL N + + F +L L L N L+ I A F+ + +L+ L + IV A F G ++LDL LT + + LH L LS NS+ ++++ F GL +L++LNL ++ + R L HL + +C H LP + L +HL + + ++ F LT L+ L L+ N+I +E SF +L L LDL FN+LT I S F+ + L+S NL P A L +L ++L GN+++ A LP+L L+L+DN + L
Sbjct: 48 PCAKGCTCLQDDYSLELNVYCSTRNFTQVPSDIPLSTRSLWLDGNLFSNLPAGAFEKLTNLEFLNLQSSLLVSLDAHAFRGLNSLAHLHLERNNLRSLSGLVF-QGTSNLATLSLNNNQMFRIDDRLFAGMSHMWLLN-LGWNLLSVLPETGFQDLHGLRELILAGNRLAYLQPQLF--QGLNELKELDLTGNYLKVIKANVFLKLP------KLQKLYLGQNQIVTVAPRAFVGMKSLRWLDLSRNRLTVLHDETFIGL--QSLHVLRLSNNSINSLRPGTFRDLQYLEELCLYHNQIRALGVRVFEGLGHLEVLNLENNRLQE--ARMGTFMGLNHLAVMKLT-GSCFHSLPDQVFKGLSKLHSIHLDRSCLTKVSSQSFSGLTGLRRLFLQHNNISLVERQSFVELQRLQQLDLRFNKLTTITSNTFYGLKNLDYLLLSGNLLHNLP-----AEALLPLQHLSWLDLSGNKLELILNA--TIYMLPRLRYLNLKDNILVNL 529 HSP 2 Score: 64.6994 bits (156), Expect = 4.411e-10 Identity = 97/332 (29.22%), Postives = 157/332 (47.29%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNE--------FEKIPVLKA-------------------THMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSK-LHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS-DPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIR 1189
LDL+ N +K + N F L L++L L N++ + AFVG+ +LR L L N L+VLH ETF GL L + L NN F + L+ H+ LNL++N+++ A + LN L + LT + + D V +KL ++ L + L +++++F + L L L+ N N ++VE+ F Q LDL+ LT+I+ + F+ K L +L LS N +L ++ +A L +L L+L+ K+ + + +L +L + DN L+N P +P SL L L NN +
Sbjct: 231 LDLTGNYLKVIKANVFLKLPKLQKLYLGQNQIVTVAPRAFVGMKSLRWLDLSRNRLTVLHDETF-IGLQSLHVLRLSNNSINSLRPGTFRDLQYLEELCLYHNQIRALGVRVFEGLGHLEVLNLENNRLQEARMGTFMGLNH-LAVMKLTGSCFHSL--PDQVFKGLSKLHSIHLDRSCLTKVSSQSFSGL-TGLRRLFLQHN---NISLVERQ--SFVELQRLQQLDLRFNKLTTITSN---TFYGLKNLDYLLLSGN-LLHNLPAEALLPLQHLSWLDLSGNKLELILNATIYMLPRLRYLNLKDNILVNLPVSIPESLDQLWLSGNNWK 548
BLAST of Epiphycan vs. Ensembl Zebrafish
Match: igfals (insulin-like growth factor binding protein, acid labile subunit [Source:ZFIN;Acc:ZDB-GENE-061009-25]) HSP 1 Score: 105.531 bits (262), Expect = 9.289e-23 Identity = 140/504 (27.78%), Postives = 216/504 (42.86%), Query Frame = 2
Query: 182 PCPIPCKCTHH------SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSM-----------------------LKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLP-------ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPK----LISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKL 1564
PC C C +V C + VP I + L L NL +L +F+ L NL+ L L + + ++ +AF GL++L +LHLE N+L L F +G L + L NN+ +I +HM LN + S + + F GL+ LIL N + + F +L L L N L+ I A F+ + +L+ L + IV A F G ++LDL LT + + LH L LS NS+ ++++ F GL +L++LNL ++ + R L HL + +C H LP + L +HL + + ++ F LT L+ L L+ N+I +E SF +L L LDL FN+LT I S F+ + L+S NL P A L +L ++L GN+++ A LP+L L+L+DN + L
Sbjct: 48 PCAKGCTCLQDDYSLELNVYCSTRNFTQVPSDIPLSTRSLWLDGNLFSNLPAGAFEKLTNLEFLNLQSSLLVSLDAHAFRGLNSLAHLHLERNNLRSLSGLVF-QGTSNLATLSLNNNQMFRIDDRLFAGMSHMWLLN-LGWNLLSVLPETGFQDLHGLRELILAGNRLAYLQPQLF--QGLNELKELDLTGNYLKVIKANVFLKLP------KLQKLYLGQNQIVTVAPRAFVGMKSLRWLDLSRNRLTVLHDETFIGL--QSLHVLRLSNNSINSLRPGTFRDLQYLEELCLYHNQIRALGVRVFEGLGHLEVLNLENNRLQE--ARMGTFMGLNHLAVMKLT-GSCFHSLPDQVFKGLSKLHSIHLDRSCLTKVSSQSFSGLTGLRRLFLQHNNISLVERQSFVELQRLQQLDLRFNKLTTITSNTFYGLKNLDYLLLSGNLLHNLP-----AEALLPLQHLSWLDLSGNKLELILNA--TIYMLPRLRYLNLKDNILVNL 529 HSP 2 Score: 64.6994 bits (156), Expect = 4.411e-10 Identity = 97/332 (29.22%), Postives = 157/332 (47.29%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNE--------FEKIPVLKA-------------------THMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSK-LHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS-DPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIR 1189
LDL+ N +K + N F L L++L L N++ + AFVG+ +LR L L N L+VLH ETF GL L + L NN F + L+ H+ LNL++N+++ A + LN L + LT + + D V +KL ++ L + L +++++F + L L L+ N N ++VE+ F Q LDL+ LT+I+ + F+ K L +L LS N +L ++ +A L +L L+L+ K+ + + +L +L + DN L+N P +P SL L L NN +
Sbjct: 231 LDLTGNYLKVIKANVFLKLPKLQKLYLGQNQIVTVAPRAFVGMKSLRWLDLSRNRLTVLHDETF-IGLQSLHVLRLSNNSINSLRPGTFRDLQYLEELCLYHNQIRALGVRVFEGLGHLEVLNLENNRLQEARMGTFMGLNH-LAVMKLTGSCFHSL--PDQVFKGLSKLHSIHLDRSCLTKVSSQSFSGL-TGLRRLFLQHN---NISLVERQ--SFVELQRLQQLDLRFNKLTTITSN---TFYGLKNLDYLLLSGN-LLHNLPAEALLPLQHLSWLDLSGNKLELILNATIYMLPRLRYLNLKDNILVNLPVSIPESLDQLWLSGNNWK 548
BLAST of Epiphycan vs. Ensembl Zebrafish
Match: lgr4 (Danio rerio leucine-rich repeat containing G protein-coupled receptor 4 (lgr4), mRNA. [Source:RefSeq mRNA;Acc:NM_001353862]) HSP 1 Score: 98.2117 bits (243), Expect = 2.869e-20 Identity = 125/465 (26.88%), Postives = 198/465 (42.58%), Query Frame = 2
Query: 185 CPIPCKCTH-HSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-----------------------PETFNKGLIKLLHVYLGNNEFEKIPVLKATH---MTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF---------------------VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-NLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKI-SDPDIRFSAMAKLTHLFMNDNNLINCPH-RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKL-ISLNLEQQNPVLLTIAP 1423
C C+C DC + L VP G+S LD+S N I L N F+ L L+ L+L+ N++ I+ A GL L+ L L+NN L + PE +GL +L H++L +N ++P+ H + L L N+I + + D +F + L L L N I+EI + F KL L N++ +I AF +NSL+ + L N L F G T FQ L S LH L L SM++ D + G NL+ L L KI S P + L + ++ N++ + P + L+ ++LQ N I+QI + F+ +T+L+ L L +N I I ++F L++LT+LDL N L + + + +L ++ N+E +N ++ P
Sbjct: 32 CSPLCRCDEDGGADCSGRGLTSVPTGLSAFTYYLDISMNNITELPANVFRNLPYLEELRLAGNDLAFIHPEALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQSNLQALTLALNRI-THIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEAIQMLPKLKELGFHSNNIASIPEGAF--CRNSLLRTIHLFDNPL-----------SFVGTTAFQNL--------------------SDLHSLMLRGASMMQ--DFPSLTGTINLESLTLTGTKIRSIPADLCEDLTVLRTVDLSYNDIEDLPSFQGCVRLQDINLQHNQIKQIDRGTFQGMTSLRVLDLSRNQIKFIHRDAFLSLSALTNLDLSLNSLASVPTAGLSALNQLKLTGNMELRNGLMSKTLP 460 HSP 2 Score: 78.9518 bits (193), Expect = 2.526e-14 Identity = 92/338 (27.22%), Postives = 149/338 (44.08%), Query Frame = 2
Query: 920 FLKPFFDSKLHHLD--LSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP-----HRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQ------------QNPVLLTIA----PNSFVLS------------------------------NLESIELQGNRIKF--------------------DATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI 1711
F+ P S LH L + +N+ LK++ + A + L+ LQ L L I+ P+ F + +L HL+++DN+L P H+ ++L+ L L N I I + F +L++L LHL N I I N F+ L +L +LDL FN L I +PKL L +N +L TI P SFV + NLES+ L G +I+ D P F +L +++L+ NQI +++ F G +++++DLS N + FL L S+ ++ L++N L+++
Sbjct: 98 FIHPEALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQ--SNLQALTLALNRITHIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEAI-QMLPKLKELGFHSNNIASIPEGAFCRNSLLRTIHLFDNPLSFVGTTAFQNLSDLHSLMLRGASMMQDFPSLTGTINLESLTLTGTKIRSIPADLCEDLTVLRTVDLSYNDIEDLPSFQGCVRLQDINLQHNQIKQIDRGTFQGMTSLRVLDLSRNQIKFIHRDAFLSL-SALTNLDLSLNSLASV 431 HSP 3 Score: 66.2402 bits (160), Expect = 1.999e-10 Identity = 68/239 (28.45%), Postives = 110/239 (46.03%), Query Frame = 2
Query: 881 DLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPHRLPA---SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLF 1579
D GLTS+ + ++LD+S N++ + + + FR L L+ L LA ++ P+ S + +L L + +N L P +L+ L L +N+I + +D F+ L L+ L L NS+ + + ++L +L L NR+T I F + L+ L+L N + I N F L NLE+++L N +K A LPKL L N I + E F
Sbjct: 45 DCSGRGLTSVPTGL-----SAFTYYLDISMNNITE-LPANVFRNLPYLEELRLAGNDLAFIHPE-ALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQSNLQALTLALNRITHIPDNAFANLSSLVVLHL--HNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEA---IQMLPKLKELGFHSNNIASIPEGAF 271 HSP 4 Score: 56.9954 bits (136), Expect = 1.345e-7 Identity = 54/171 (31.58%), Postives = 89/171 (52.05%), Query Frame = 2
Query: 1154 LKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGP--IFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWP-AKTFLP 1657
L+ L L N++ I + L LK L L+ N + ++ + + +L +L SL L N +T + F + +L L L+ + + I+P SNL+++ L NRI T P F NL LV L L +N+I ++ +N F+G N++ +DL+ N+L +P A LP
Sbjct: 86 LEELRLAGNDLAFIHPEALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQ-SNLQALTLALNRI----THIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEAIQMLP 251
BLAST of Epiphycan vs. Ensembl Zebrafish
Match: tlr5a (toll-like receptor 5a [Source:ZFIN;Acc:ZDB-GENE-040219-14]) HSP 1 Score: 98.2117 bits (243), Expect = 3.190e-20 Identity = 159/611 (26.02%), Postives = 250/611 (40.92%), Query Frame = 2
Query: 197 CKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMK-EINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLG---------NNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTG-LQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTG--FNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQN----------PVL--LTIAPNSF--------VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKL-NENLFSGSVNIQLIDLSMNSL-IGWPAKTFLPLI-----------------SSAIDIRLNMNKLSTISIDFNSTNYLPID-----------------------FAKLKNISLENNQWNCSC 1804
C + C + L++VPK IS +T +DLS N I L+ SF L L+ L L H I N+F+ LSNL +L L+ N + FN GL L ++ L + F K P++ + L+L N IR V SF LN L LT N++K I + D +N + +L +++ + + E GN N++I L+G FN +F D +IS ++ S + + S K D F GL + I D+ S++ LT+ + + L+ + L + I +I + F +TNL +L+L KN + I+ +F +L L LDL +N + R+ S F +P L+SLNL + P L L + N+ + +L++++LQ N+I + I D P++ + R NQ+ +N S NIQ++DLS L + W L + S DI ++ L + + FNS YLP D F+ L +SL NN + C C
Sbjct: 25 CSTDGYFAFCMDRGLQEVPK-ISTYITNVDLSKNRIAELNETSFSHLEALEVLILMHQTPGLVIRRNSFMRLSNLTSLQLDYNHHLRIDAGAFN-GLSDLKNLTLTQCGLDDSLLSGNFLK-PLMS---LEMLDLSRNNIRR-VQPASFFLNMRRFHVLDLTFNKVKSICEEDLLNFQGKHF--------TLLRLSSITLQDMNEYWLGWEKCGNPFKNTSITTLDLSGNGFNVDMAKRFFD-------AISSTKIQSLIISNTYRIGKSSGDNSKDPDKSTFSGLKS--------SGIKSFDLSNSSIFSLTYSVFS----------YLSDLEQITLAESQINKIENNAFLGMTNLLKLNLSKNFLGYIDFRTFQNLKGLEVLDLSYNHIWRLGSQSFQGLPNLLSLNLTGNSLGHVYTFATLPKLEKLYLGDNNIQYVYEIPNIAKHLKTLDLQFNQIASMSELYTILDEFPQIEEVVFRGNQLLYCPQDNHEVLSQNIQVLDLSFAGLQVIWSEGKCLNVFDDLHQLEVLHLSSNLLQSLPKDIFKDLTSLLILDLSFNSLKYLPTDVFPKTLQVLHLDYNSIYSVDPNLFSTLSYLSLMNNDFRCDC 595
BLAST of Epiphycan vs. Ensembl Xenopus
Match: rpl24 (ribosomal protein L24 [Source:Xenbase;Acc:XB-GENE-963922]) HSP 1 Score: 114.775 bits (286), Expect = 3.442e-25 Identity = 128/414 (30.92%), Postives = 185/414 (44.69%), Query Frame = 2
Query: 185 CPIPCKCTHHS----VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLR-----NLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKL---NLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQ--ILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPH----RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKL 1372
CP PC+C VDC +AL +P+ +SI + LDLS N I +L +N L L+ L+L+ N++ I + AF GL +L+ L L+ N +S + P +FN GL L H++L +N +IPV ++ L L NKI + D +F + L L L N I + + F L TL L N+L+ A A +KN L L N + +I EQA G + L +I F+D+ + H + AF+ L L+ ILN A PD+ + L L + L+ P +LP +L+ L L+ N + +IR F+ L L+ L L N I I NSF L SL LDL N LT H + L
Sbjct: 32 CPTPCECEQEGMLVRVDCSDRALTSLPRNLSIFTSYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLPLIKLRLDANHISYVPPSSFN-GLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHH-IPDYAFRNLSSLVVLHLHNNRIYSLGKKCF--DGLHSLETLDLNYNNLDEFPA-AIKTLKN-LKELGFHSNNI--KSIPEQAFIG-------------NPSLITIH------FYDNPIQH-----------VGRSAFQHLPELRTLILNGASQITEFPDL--TGTTSLESLTLTGAQLVYLPSAVCTQLP-NLQVLDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSLPSLIKLDLSSNHLTSFPVTGLHGLTHL 404 HSP 2 Score: 76.6406 bits (187), Expect = 1.655e-13 Identity = 86/311 (27.65%), Postives = 134/311 (43.09%), Query Frame = 2
Query: 947 LHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD------PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH---LQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGI----------FHR-------------MPKLISLNLE------------QQNPVLLTIA----------PNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISI 1717
L L L+ N L I AF GL +L++L L K ++ P F+ + L HL+++DN+L P R SL L L N I I F++L++L LHL N I S+ FD L SL +LDL +N L + I FH P LI+++ Q P L T+ P+ ++LES+ L G ++ + +A + LP L L LR N++ ++ F V ++ +DL+ N + +F L S I + L+ N L++ +
Sbjct: 89 LEELRLAGND-LTYIPKGAFAGLGSLKVLPLIKLRLDANHISYVPPSSFNGLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHHIPDYAFRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAAIKTLKNLKELGFHSNNIKSIPEQAFIGNPSLITIHFYDNPIQHVGRSAFQHLPELRTLILNGASQITEFPDLTGTTSLESLTLTGAQLVYLPSA--VCTQLPNLQVLDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSL-PSLIKLDLSSNHLTSFPV 395
BLAST of Epiphycan vs. Ensembl Xenopus
Match: IGFALS (insulin like growth factor binding protein acid labile subunit [Source:NCBI gene;Acc:100489984]) HSP 1 Score: 110.923 bits (276), Expect = 2.188e-24 Identity = 160/539 (29.68%), Postives = 245/539 (45.45%), Query Frame = 2
Query: 212 HSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSF--LLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFD-SKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIR-FSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSC 1804
+SV C + L VP+ L L N + ++ +F+ L +L L L +++ + + A GL L +LHLE N + L P TF L+ + L NN KI +++ LNL N + + D F LLN L+ L+L N + + + F++L K L+ NSL+ I A F + + L L N + ST+ +A F+G ++LDL LT++ D FF L+ L LS NS L S+ F+ L L LNL + KI R F +A+L + L L NN+++IR F L N+ ++L N + S+ + F L L SL + + L+ I+ +F + + L L QN ++ I +SF L L ++L+ N++ T F L L L L NQI ++ +FS +Q +DLS N L FLPL SS + L N L TIS++F I ++ + L NQW+C C
Sbjct: 49 YSVFCSSRNLTRVPEPPPPAARSLWLDGNNLTTVQAGAFRNLSHLDFLNLQSSQVASLEQQALHGLKALAHLHLERNMIKSLAPSTFTHT-QNLVSLTLNNNFLTKIEDGLFSGLSNLWYLNLGWN-LLVVLPDMVFHDLLN--LRELVLAGNRLVYLQPSLFLSLGELKELDLSG--NSLKGIKANVFTRQQKT-HKLYLNHNHI--STVAPRA---FSGMKNLRWLDLSHNRLTALYED---TFFGLPSLNVLRLSNNS-LTSLRPRIFKDLQFLVELNLGQNKIKILLERTFEGLAQL---------------------ELLSLNHNNVQEIRPGSFLGLLNVAVINLSGNCLKSLAEHCFKGLGKLHSLHMESSCLSHIKPQMFAGLSSIRRLFL--QNNEIVAIENHSFTDLHGLLELDLRSNKLTHLTTRS--FTGLKNLSYLLLSSNQILTISPEVFSPVQQLQWLDLSDNQLKALTEDIFLPL-SSLRYLSLKNNCLKTISVNF------LIPPPTMQQLWLNGNQWDCGC 539
BLAST of Epiphycan vs. Ensembl Xenopus
Match: rpl24 (ribosomal protein L24 [Source:Xenbase;Acc:XB-GENE-963922]) HSP 1 Score: 108.997 bits (271), Expect = 2.013e-23 Identity = 129/433 (29.79%), Postives = 186/433 (42.96%), Query Frame = 2
Query: 185 CPIPCKCTHHS----VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLEN------------------------NDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKL---NLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQ--ILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPH----RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKL 1372
CP PC+C VDC +AL +P+ +SI + LDLS N I +L +N L L+ L+L+ N++ I + AF GL +L+ L L+N N +S + P +FN GL L H++L +N +IPV ++ L L NKI + D +F + L L L N I + + F L TL L N+L+ A A +KN L L N + +I EQA G + L +I F+D+ + H + AF+ L L+ ILN A PD+ + L L + L+ P +LP +L+ L L+ N + +IR F+ L L+ L L N I I NSF L SL LDL N LT H + L
Sbjct: 32 CPTPCECEQEGMLVRVDCSDRALTSLPRNLSIFTSYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLMLQNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFN-GLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHH-IPDYAFRNLSSLVVLHLHNNRIYSLGKKCF--DGLHSLETLDLNYNNLDEFPA-AIKTLKN-LKELGFHSNNI--KSIPEQAFIG-------------NPSLITIH------FYDNPIQH-----------VGRSAFQHLPELRTLILNGASQITEFPDL--TGTTSLESLTLTGAQLVYLPSAVCTQLP-NLQVLDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSLPSLIKLDLSSNHLTSFPVTGLHGLTHL 423 HSP 2 Score: 75.8702 bits (185), Expect = 3.546e-13 Identity = 82/299 (27.42%), Postives = 132/299 (44.15%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH---LQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGI----------FHR-------------MPKLISLNLE------------QQNPVLLTIA----------PNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISI 1717
+N++L+ + ++A L +LQ L L IS P F+ + L HL+++DN+L P R SL L L N I I F++L++L LHL N I S+ FD L SL +LDL +N L + I FH P LI+++ Q P L T+ P+ ++LES+ L G ++ + +A + LP L L LR N++ ++ F V ++ +DL+ N + +F L S I + L+ N L++ +
Sbjct: 119 QNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFNGLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHHIPDYAFRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAAIKTLKNLKELGFHSNNIKSIPEQAFIGNPSLITIHFYDNPIQHVGRSAFQHLPELRTLILNGASQITEFPDLTGTTSLESLTLTGAQLVYLPSA--VCTQLPNLQVLDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSL-PSLIKLDLSSNHLTSFPV 414
BLAST of Epiphycan vs. Ensembl Xenopus
Match: rpl24 (ribosomal protein L24 [Source:Xenbase;Acc:XB-GENE-963922]) HSP 1 Score: 108.612 bits (270), Expect = 2.966e-23 Identity = 138/484 (28.51%), Postives = 210/484 (43.39%), Query Frame = 2
Query: 185 CPIPCKCTHHS----VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLEN------------------------NDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKL---NLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQ--ILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPH----RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKL 1525
CP PC+C VDC +AL +P+ +SI + LDLS N I +L +N L L+ L+L+ N++ I + AF GL +L+ L L+N N +S + P +FN GL L H++L +N +IPV ++ L L NKI + D +F + L L L N I + + F L TL L N+L+ A A +KN L L N + +I EQA G + L +I F+D+ + H + AF+ L L+ ILN A PD+ + L L + L+ P +LP +L+ L L N+I+ + F L+++ L+ N + I + +F L L SLDL +N++ I F +P LI L+L + LT P + L L ++L GN D ++ PKL
Sbjct: 32 CPTPCECEQEGMLVRVDCSDRALTSLPRNLSIFTSYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLMLQNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFN-GLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHH-IPDYAFRNLSSLVVLHLHNNRIYSLGKKCF--DGLHSLETLDLNYNNLDEFPA-AIKTLKN-LKELGFHSNNI--KSIPEQAFIG-------------NPSLITIH------FYDNPIQH-----------VGRSAFQHLPELRTLILNGASQITEFPDL--TGTTSLESLTLTGAQLVYLPSAVCTQLP-NLQVLDLSYNHIKDL--PSFSGCQRLQKIDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSLPSLIKLDLSSNH---LTSFPVT-GLHGLTHLKLTGNSALQDLIPS---EHFPKL 465 HSP 2 Score: 73.559 bits (179), Expect = 1.874e-12 Identity = 86/297 (28.96%), Postives = 128/297 (43.10%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH---LQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGI----------FHRM-------------PKLISLNLE------------QQNPVLLTIA----------PNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI 1711
+N++L+ + ++A L +LQ L L IS P F+ + L HL+++DN+L P R SL L L N I I F++L++L LHL N I S+ FD L SL +LDL +N L + I FH P LI+++ Q P L T+ P+ ++LES+ L G ++ + +A + LP L L L N I L FSG +Q IDL N + + TF L+ + L NK++ I
Sbjct: 119 QNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFNGLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHHIPDYAFRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAAIKTLKNLKELGFHSNNIKSIPEQAFIGNPSLITIHFYDNPIQHVGRSAFQHLPELRTLILNGASQITEFPDLTGTTSLESLTLTGAQLVYLPSA--VCTQLPNLQVLDLSYNHIKDLPS--FSGCQRLQKIDLRHNEVYEIRSTTFQQLVGLR-SLDLAWNKIAVI 410 HSP 3 Score: 56.6102 bits (135), Expect = 3.013e-7 Identity = 56/189 (29.63%), Postives = 95/189 (50.26%), Query Frame = 2
Query: 1094 THLFMNDNNLINCPHRLPASLKFL---HLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAK 1645
++L ++ NN+ N P + +L FL L N++ I + F L +LK L L+ N + + + + +L SL SL L N ++ + F+ + L L L+ + LT P + LS L+++ L N+I F NL LV L L +N+I L + F G +++ +DL+ N+L +PA
Sbjct: 66 SYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLMLQNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFNGLFSLRHLWLDDNS---LTEIPVRALESLSALQAMTLALNKIHHIPDYA--FRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAA 249
BLAST of Epiphycan vs. Ensembl Xenopus
Match: ENSXETT00000011039.1 (toll like receptor 3 [Source:NCBI gene;Acc:100486223]) HSP 1 Score: 106.686 bits (265), Expect = 1.009e-22 Identity = 183/727 (25.17%), Postives = 279/727 (38.38%), Query Frame = 2
Query: 128 FTFC------LLLVIYNLVCLYVFPCPI--PCKCTHH-SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLS-------------NNSFQTL-----------INLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNK--GLI-------KLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDK-SFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DP-------------------------DIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQ--IRQDDFKSLTN--LKELHLKKNSILSIETNSFDDLASLTSLDLG-------------------------FNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIK------FDA----------------------TAGPI--FDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID-FNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHF-----RKLILNRDQILCNIPNEKAG 1891
F+FC L L ++ L +Y PCK T + DC H L +P + ++ LDLS N +K L + F TL + LK L L HNE +I+EN F +L LHL +N +S + F K L+ K++ LGN + + ++ +L L+SNKI + FL NT LQ L L+ N + EIN F + I + A + ++N+ L+ EQ + TG L L + L+ I K D+ L LDLS NS L IDND+F L L+ILNL IS +P D+ F ++ + +L M N +I SL L L + ++Q + F SL+ L L+L K + IE +F L L LD+G +NR + S F P L L L + + P+ F VL NL ++L N I F++ GP+ L L LSL N ++ N F G ++++D+ N++ P F S + + L+ N ++++ D F + F+ LK +++ N ++C+C I W + + L Q +CN P G
Sbjct: 16 FSFCRNEMGQLWLSLHILYAIYGMSLVAGNPCKITEDLTADCSHLKLSAIPSDLPRNIKALDLSHNQLKKLPAANLSVYDQLERLDAGFNTLHQFEPALCKQLLMLKTLNLEHNEFTKISENDFTFCVHLSELHLASNGISNIDGNPFEKLENLLFLDMSHNKMISTALGNKQ-------QLNNLKELYLNSNKISKLNKEAFEFLANTSLQKLDLSSNPLTEINPGCF------------------QAIGSFAALVIQNT----------QLDPQRTEQVCSEL-AHTGVLNLTLNNIRLSKIHNTTFKGLADTNLTVLDLSGNS-LSQIDNDSFVSLGALEILNLENNDISHVNPKTFNGLSKVRVLNLRKSLSSNLKLDDLSFQSLHNVQYLNMEANKMIAITEHTFIGLTSLTNLSLSDSTLQQHTLTNRTFLSLSKSPLYHLNLTKTGLTKIENGAFLCLKHLQLLDMGLNQIDQEVSGNELEGLANIEMIYLSYNRRITLTSNSFIFAPSLSKLYLRKTALTFRDLNPSPFKVLQNLTVLDLSNNNIANIQEDVFESLSNLRILSFEHNNLARLWKEANPGGPVLFLKGLKNLEKLSLLSNGFDEIPANAFKGLCKLKVLDIGENNVYILPPSVFDDQHSLTL-LDLHKNLITSVEQDLFKNV------FSSLKYLNMGGNPYDCTCESIAWFASWLNTTNTSVPLAHSQYICNTPARYHG 698
BLAST of Epiphycan vs. Ensembl Mouse
Match: Igfals (insulin-like growth factor binding protein, acid labile subunit [Source:MGI Symbol;Acc:MGI:107973]) HSP 1 Score: 108.227 bits (269), Expect = 1.336e-23 Identity = 137/504 (27.18%), Postives = 225/504 (44.64%), Query Frame = 2
Query: 185 CPIPCKCTHH------SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFN-----------------------KGLIKLLHVYLGNNEFEKIP--VLKA-THMTKLNLDSNKIRSAVVDKSFLLNTG-LQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLD---LSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHL-FMNDNNLINCPHRLP-------ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLT---IAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQI 1555
CP+ C C++ SV C + L +P I + L L N + S+ + +FQ L +L L L + ++ + A +GL NL +LHLE N L L F +GL L + LG N +P V + ++ +L L NK+ + + L G L+ L L++N ++ + F++L + L N + +A AF+ +K +L L+L N++ + ++E F G G L L +TS L+P LH L+ L N + + + F GL L++L L +I + ++ A L ++ MN + NC LP L LHL+ + + +IR F L+ L+ L L+ NSI SIE S L+ L LDL N+LT + +F + +L L L +L+ + P L ++L NR++ A +F +L +L L+LR+N +
Sbjct: 41 CPVTCTCSYDDYTDELSVFCSSRNLTQLPDSIPVSTRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRHTPSLASLSLGNNLLGRLEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKL--TYLQPALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDR--NLITAVAPRAFLGMK-ALRWLDLSHNRV--AGLLEDT---FPGLLGLHVLRLAHNAITS-----LRPRTFKDLHFLEELQLGHNRI-RQLGEKTFEGLGQLEVLTLNDNQIHE--VKVGAFFGLFNVAVMNLSG--NCLRSLPEHVFQGLGRLHSLHLEHSCLGRIRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTANQLTHLPRQLFQGLGQLEYLLLSNNQLTMLSEDVLGP----LQRAFWLDLSHNRLETPAEG--LFSSLGRLRYLNLRNNSL 518 HSP 2 Score: 80.4925 bits (197), Expect = 6.794e-15 Identity = 91/313 (29.07%), Postives = 147/313 (46.96%), Query Frame = 2
Query: 731 NSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLA-KCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLP---ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLN-LEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTF 1651
N+L +I + AF + +SL L L+G+ L ++ QAL G L L+ L S++ + L L L N++L ++ FRGL +L LNL + PD F + L L + N L L L+ L L N +R ++ + F L L++L+L +N I ++ +F + +L LDL NR+ + F P L+ L+ L + + ++ P +F L LE ++L NRI+ F+ L +L L+L DNQI ++ F G N+ +++LS N L P F
Sbjct: 84 NNLSSIPSAAFQNL-SSLDFLNLQGSWL--RSLEPQALLGLQN---LYHLHLERNLLRSLAAGLFR--HTPSLASLSLG-NNLLGRLEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTF---PGLLGLHVLRLAHNAITSLRPRTFKDLHFLEELQLGHNRIR--QLGEKTFEGLGQLEVLTLNDNQIHEVKVGAFFGLFNVAVMNLSGNCLRSLPEHVF 382 HSP 3 Score: 77.7962 bits (190), Expect = 4.088e-14 Identity = 68/231 (29.44%), Postives = 107/231 (46.32%), Query Frame = 2
Query: 1100 LFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATA----------------------GPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI 1711
L+++ NNL + P +SL FL+LQ + +R + L NL LHL++N + S+ F SL SL LG N L R+E G+F + L LNL + L + P++ L NL + L GN++ + A +F +LP+L L L N IT + F G ++ +DLS N + G TF L+ + +RL N ++++
Sbjct: 79 LWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRHTPSLASLSLGNNLLGRLEEGLFRGLSHLWDLNLGWNS---LVVLPDTVFQGLGNLHELVLAGNKLTYLQPALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTFPGLLGLHV-LRLAHNAITSL 305
BLAST of Epiphycan vs. Ensembl Mouse
Match: Lgr4 (leucine-rich repeat-containing G protein-coupled receptor 4 [Source:MGI Symbol;Acc:MGI:1891468]) HSP 1 Score: 101.679 bits (252), Expect = 2.776e-21 Identity = 113/402 (28.11%), Postives = 186/402 (46.27%), Query Frame = 2
Query: 185 CPIPCKC-THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMT---KLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF--------VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-NLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPH-RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIES 1345
C PC C VDC + L VP+G+S L L+ N + + + L LK L L +N++K + A GLS L++L L+ N ++ + PE +GL++L H++L +N ++PV +++ L L N I S++ D +F + L L L N+IK ++Q F ++L L + +L ++ F S+I + GN LL + + F G + F L S LH L + S+++ N A G +L+ L L KIS PD L L ++ N++ + P +L+ + LQ N I I++ F+ LT+L+ L L +N I I + +F L ++T+LD+ FN LT +
Sbjct: 29 CAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSV-PEDSFEGLVQLRHLWLDDNILTEVPVRPLSNLPTLQALTLALNNI-SSIPDFAFTNLSSLVVLHLHNNKIKSLSQHCFDGLDNLETLDLNYNNLDEFPQAIKALPSLKELGFHSNSISVIPDGAFAGNPLLRTIHLYDNPLSFVGNSAFHNL--------------------SDLHSLVIRGASLVQWFPNLA--GTVHLESLTLTGTKISSIPDDLCQNQKMLRTLDLSYNDIRDLPSFNGCRALEEISLQRNQISLIKETTFQGLTSLRILDLSRNLIREIHSGAFAKLGTITNLDVSFNELTSFPT 406 HSP 2 Score: 88.5817 bits (218), Expect = 3.548e-17 Identity = 85/283 (30.04%), Postives = 135/283 (47.70%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHR----LPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSN--LESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPI--DFAKLKNISLENNQ 1789
+N+ LK++ ++A RGL LQ L L I+ P+ F + +L HL+++DN L P R LP +L+ L L NNI I F +L++L LHL N I S+ + FD L +L +LDL +N L I +P L L + +++ P+ N L +I L N + F + F NL L +L +R + + NL +G+V+++ + L+ + P D+ N L T+ + +N LP L+ ISL+ NQ
Sbjct: 89 QNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNILTEVPVRPLSNLP-TLQALTLALNNISSIPDFAFTNLSSLVVLHLHNNKIKSLSQHCFDGLDNLETLDLNYNNLDEFPQAI-KALPSLKELGFHSNS---ISVIPDGAFAGNPLLRTIHLYDNPLSFVGNSA--FHNLSDLHSLVIRGASLVQWFPNL-AGTVHLESLTLTGTKISSIPD-----------DLCQNQKMLRTLDLSYNDIRDLPSFNGCRALEEISLQRNQ 352
BLAST of Epiphycan vs. Ensembl Mouse
Match: Lgr4 (leucine-rich repeat-containing G protein-coupled receptor 4 [Source:MGI Symbol;Acc:MGI:1891468]) HSP 1 Score: 100.908 bits (250), Expect = 4.861e-21 Identity = 116/425 (27.29%), Postives = 188/425 (44.24%), Query Frame = 2
Query: 185 CPIPCKC-THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-----------------------PETFNKGLIKLLHVYLGNNEFEKIPVLKATHMT---KLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF--------VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-NLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPH-RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIES 1345
C PC C VDC + L VP+G+S LD+S N I L ++F+ L+ L+L+ N++ I+ A GL L+ L L+NN L + PE +GL++L H++L +N ++PV +++ L L N I S++ D +F + L L L N+IK ++Q F ++L L + +L ++ F S+I + GN LL + + F G + F L S LH L + S+++ N A G +L+ L L KIS PD L L ++ N++ + P +L+ + LQ N I I++ F+ LT+L+ L L +N I I + +F L ++T+LD+ FN LT +
Sbjct: 29 CAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNILTEVPVRPLSNLPTLQALTLALNNI-SSIPDFAFTNLSSLVVLHLHNNKIKSLSQHCFDGLDNLETLDLNYNNLDEFPQAIKALPSLKELGFHSNSISVIPDGAFAGNPLLRTIHLYDNPLSFVGNSAFHNL--------------------SDLHSLVIRGASLVQWFPNLA--GTVHLESLTLTGTKISSIPDDLCQNQKMLRTLDLSYNDIRDLPSFNGCRALEEISLQRNQISLIKETTFQGLTSLRILDLSRNLIREIHSGAFAKLGTITNLDVSFNELTSFPT 430 HSP 2 Score: 88.5817 bits (218), Expect = 3.343e-17 Identity = 85/283 (30.04%), Postives = 135/283 (47.70%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHR----LPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSN--LESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPI--DFAKLKNISLENNQ 1789
+N+ LK++ ++A RGL LQ L L I+ P+ F + +L HL+++DN L P R LP +L+ L L NNI I F +L++L LHL N I S+ + FD L +L +LDL +N L I +P L L + +++ P+ N L +I L N + F + F NL L +L +R + + NL +G+V+++ + L+ + P D+ N L T+ + +N LP L+ ISL+ NQ
Sbjct: 113 QNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNILTEVPVRPLSNLP-TLQALTLALNNISSIPDFAFTNLSSLVVLHLHNNKIKSLSQHCFDGLDNLETLDLNYNNLDEFPQAI-KALPSLKELGFHSNS---ISVIPDGAFAGNPLLRTIHLYDNPLSFVGNSA--FHNLSDLHSLVIRGASLVQWFPNL-AGTVHLESLTLTGTKISSIPD-----------DLCQNQKMLRTLDLSYNDIRDLPSFNGCRALEEISLQRNQ 376
BLAST of Epiphycan vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 99.7525 bits (247), Expect = 1.457e-20 Identity = 158/643 (24.57%), Postives = 274/643 (42.61%), Query Frame = 2
Query: 176 VFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDS---NKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKS-----CGLTSISGDFLK--------PFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDL---GFNRLTRIES-GIFHRMPKLISLNLEQQNPVLLTIAP---------------------------------NSFVLSNLESIELQGNRIKFDATA----GPIFDNLPK-------LVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID-FNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQIL-CNIPNEKAGK 1894
V CP C C+++ VDCR + L ++P + +T + L N I+ + +F L+RL LS+N++ E+ +AF GL +L +L L N ++ L P++ +GL L + L N+ + V + LNL S NK+++ V +F +QT+ L +N F+ + K L N +E A P + + + +K + E + +G F DL C T++ + P + ++L L+ +E ++L++ F+ L L+ +N + KI+D + F + + + + N L N H++ SLK L L+SN I + D F L +++ L L N I ++ +FD L SL++L+L FN + G + R ++++ N Q P L P ++ V + + +++ I D T G F +PK L + L +N+I+ L+ FS + + LS N L P +TF L S + + L+ N +S + FN D + L ++++ N C C+ +QWL + K I C P E A K
Sbjct: 274 VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITEL-PKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQT-VAKGTFSALRAIQTMHLAQNP--------FICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDC---FADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAEL-RLNNNEFTVLEAT--GIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRL-LSLHGNDISVVPEGAFN-------DLSALSHLAIGANPLYCDCN-MQWLSDWVKSEYKEPGIARCAGPGEMADK 891 HSP 2 Score: 77.411 bits (189), Expect = 9.174e-14 Identity = 57/170 (33.53%), Postives = 80/170 (47.06%), Query Frame = 2
Query: 185 CPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF 685
CP C C+ +VDC AL+ VP+ I + LDL+ N I ++ F L +L+ L+L N + I AF L L L L N+L L PE G KL + L N+ + IP A + L LD N+I S + D +F L+ L L N I ++ F
Sbjct: 28 CPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQ-LFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQI-SCIEDGAFRALRDLEVLTLNNNNITRLSVASF 195 HSP 3 Score: 53.1434 bits (126), Expect = 2.978e-6 Identity = 58/230 (25.22%), Postives = 88/230 (38.26%), Query Frame = 2
Query: 1133 PHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWL 1822
P +P + + L L NNI +I + DF L +L+ L L +N I +IE +F DL L L L N L +F KL L+L + NQI + F G+V+I+ + L N + F L + + LN N ++ +S+ S N++P KL+ L +N C CH + WL
Sbjct: 50 PRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSE---------------------------------------------------NQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEV-LTLNNNNITRLSV--ASFNHMP----KLRTFRLHSNNLYCDCH-LAWL 220 HSP 4 Score: 51.2174 bits (121), Expect = 8.933e-6 Identity = 37/123 (30.08%), Postives = 58/123 (47.15%), Query Frame = 2
Query: 167 CLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNS-FQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI 532
C CP C+C +VDC +Q L +P I L L+ N L F+ L L+++ S+N++ +I E AF G S + + L +N L + + F KGL L + L +N +
Sbjct: 496 CFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMF-KGLESLKTLMLRSNRISCV 617
BLAST of Epiphycan vs. Ensembl Mouse
Match: Slit2 (slit guidance ligand 2 [Source:MGI Symbol;Acc:MGI:1315205]) HSP 1 Score: 99.7525 bits (247), Expect = 1.584e-20 Identity = 158/643 (24.57%), Postives = 274/643 (42.61%), Query Frame = 2
Query: 176 VFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDS---NKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKS-----CGLTSISGDFLK--------PFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDL---GFNRLTRIES-GIFHRMPKLISLNLEQQNPVLLTIAP---------------------------------NSFVLSNLESIELQGNRIKFDATA----GPIFDNLPK-------LVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID-FNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQIL-CNIPNEKAGK 1894
V CP C C+++ VDCR + L ++P + +T + L N I+ + +F L+RL LS+N++ E+ +AF GL +L +L L N ++ L P++ +GL L + L N+ + V + LNL S NK+++ V +F +QT+ L +N F+ + K L N +E A P + + + +K + E + +G F DL C T++ + P + ++L L+ +E ++L++ F+ L L+ +N + KI+D + F + + + + N L N H++ SLK L L+SN I + D F L +++ L L N I ++ +FD L SL++L+L FN + G + R ++++ N Q P L P ++ V + + +++ I D T G F +PK L + L +N+I+ L+ FS + + LS N L P +TF L S + + L+ N +S + FN D + L ++++ N C C+ +QWL + K I C P E A K
Sbjct: 270 VLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITEL-PKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQT-VAKGTFSALRAIQTMHLAQNP--------FICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDC---FADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAEL-RLNNNEFTVLEAT--GIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRL-LSLHGNDISVVPEGAFN-------DLSALSHLAIGANPLYCDCN-MQWLSDWVKSEYKEPGIARCAGPGEMADK 887 HSP 2 Score: 77.411 bits (189), Expect = 9.324e-14 Identity = 57/170 (33.53%), Postives = 80/170 (47.06%), Query Frame = 2
Query: 185 CPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF 685
CP C C+ +VDC AL+ VP+ I + LDL+ N I ++ F L +L+ L+L N + I AF L L L L N+L L PE G KL + L N+ + IP A + L LD N+I S + D +F L+ L L N I ++ F
Sbjct: 28 CPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQ-LFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQI-SCIEDGAFRALRDLEVLTLNNNNITRLSVASF 195 HSP 3 Score: 53.1434 bits (126), Expect = 2.952e-6 Identity = 58/230 (25.22%), Postives = 88/230 (38.26%), Query Frame = 2
Query: 1133 PHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWL 1822
P +P + + L L NNI +I + DF L +L+ L L +N I +IE +F DL L L L N L +F KL L+L + NQI + F G+V+I+ + L N + F L + + LN N ++ +S+ S N++P KL+ L +N C CH + WL
Sbjct: 50 PRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSE---------------------------------------------------NQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEV-LTLNNNNITRLSV--ASFNHMP----KLRTFRLHSNNLYCDCH-LAWL 220 HSP 4 Score: 51.6026 bits (122), Expect = 8.633e-6 Identity = 37/123 (30.08%), Postives = 58/123 (47.15%), Query Frame = 2
Query: 167 CLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNS-FQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI 532
C CP C+C +VDC +Q L +P I L L+ N L F+ L L+++ S+N++ +I E AF G S + + L +N L + + F KGL L + L +N +
Sbjct: 492 CFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMF-KGLESLKTLMLRSNRISCV 613
BLAST of Epiphycan vs. UniProt/SwissProt
Match: sp|P70389|ALS_MOUSE (Insulin-like growth factor-binding protein complex acid labile subunit OS=Mus musculus OX=10090 GN=Igfals PE=1 SV=1) HSP 1 Score: 111.309 bits (277), Expect = 1.063e-23 Identity = 138/504 (27.38%), Postives = 226/504 (44.84%), Query Frame = 2
Query: 185 CPIPCKCTHH------SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFN-----------------------KGLIKLLHVYLGNNEFEKIP--VLKA-THMTKLNLDSNKIRSAVVDKSFLLNTG-LQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLD---LSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHL-FMNDNNLINCPHRLP-------ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLT---IAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQI 1555
CP+ C C++ SV C + L +P GI + L L N + S+ + +FQ L +L L L + ++ + A +GL NL +LHLE N L L F +GL L + LG N +P V + ++ +L L NK+ + + L G L+ L L++N ++ + F++L + L N + +A AF+ +K +L L+L N++ + ++E F G G L L +TS L+P LH L+ L N + + + F GL L++L L +I + ++ A L ++ MN + NC LP L LHL+ + + +IR F L+ L+ L L+ NSI SIE S L+ L LDL N+LT + +F + +L L L +L+ + P L ++L NR++ A +F +L +L L+LR+N +
Sbjct: 41 CPVTCTCSYDDYTDELSVFCSSRNLTQLPDGIPVSTRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRHTPSLASLSLGNNLLGRLEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKL--TYLQPALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDR--NLITAVAPRAFLGMK-ALRWLDLSHNRV--AGLLEDT---FPGLLGLHVLRLAHNAITS-----LRPRTFKDLHFLEELQLGHNRI-RQLGEKTFEGLGQLEVLTLNDNQIHE--VKVGAFFGLFNVAVMNLSG--NCLRSLPEHVFQGLGRLHSLHLEHSCLGRIRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTANQLTHLPRQLFQGLGQLEYLLLSNNQLTMLSEDVLGP----LQRAFWLDLSHNRLETPAEG--LFSSLGRLRYLNLRNNSL 518 HSP 2 Score: 80.4925 bits (197), Expect = 4.877e-14 Identity = 91/313 (29.07%), Postives = 147/313 (46.96%), Query Frame = 2
Query: 731 NSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLA-KCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLP---ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLN-LEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTF 1651
N+L +I + AF + +SL L L+G+ L ++ QAL G L L+ L S++ + L L L N++L ++ FRGL +L LNL + PD F + L L + N L L L+ L L N +R ++ + F L L++L+L +N I ++ +F + +L LDL NR+ + F P L+ L+ L + + ++ P +F L LE ++L NRI+ F+ L +L L+L DNQI ++ F G N+ +++LS N L P F
Sbjct: 84 NNLSSIPSAAFQNL-SSLDFLNLQGSWL--RSLEPQALLGLQN---LYHLHLERNLLRSLAAGLFR--HTPSLASLSLG-NNLLGRLEEGLFRGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTF---PGLLGLHVLRLAHNAITSLRPRTFKDLHFLEELQLGHNRIR--QLGEKTFEGLGQLEVLTLNDNQIHEVKVGAFFGLFNVAVMNLSGNCLRSLPEHVF 382 HSP 3 Score: 77.7962 bits (190), Expect = 2.835e-13 Identity = 68/231 (29.44%), Postives = 107/231 (46.32%), Query Frame = 2
Query: 1100 LFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATA----------------------GPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI 1711
L+++ NNL + P +SL FL+LQ + +R + L NL LHL++N + S+ F SL SL LG N L R+E G+F + L LNL + L + P++ L NL + L GN++ + A +F +LP+L L L N IT + F G ++ +DLS N + G TF L+ + +RL N ++++
Sbjct: 79 LWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYHLHLERNLLRSLAAGLFRHTPSLASLSLGNNLLGRLEEGLFRGLSHLWDLNLGWNS---LVVLPDTVFQGLGNLHELVLAGNKLTYLQPALLCGLGELRELDLSRNALRSVKANVFIHLPRLQKLYLDRNLITAVAPRAFLGMKALRWLDLSHNRVAGLLEDTFPGLLGLHV-LRLAHNAITSL 305
BLAST of Epiphycan vs. UniProt/SwissProt
Match: sp|P35859|ALS_RAT (Insulin-like growth factor-binding protein complex acid labile subunit OS=Rattus norvegicus OX=10116 GN=Igfals PE=1 SV=1) HSP 1 Score: 107.457 bits (267), Expect = 1.451e-22 Identity = 145/580 (25.00%), Postives = 235/580 (40.52%), Query Frame = 2
Query: 185 CPIPCKCTHH------SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFN-----------------------KGLIKLLHVYLGNNEFEKIP--VLKA-THMTKLNLDSNKIRSAVVDKSFLLNTG-LQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLD---LSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDI-RFSAMAKLTHLFMNDNNLINCPHRLPASL---KFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSC 1804
CP+ C C+H SV C + L +P I + L L N + S+ + +FQ L +L L L + ++ + A +GL NL LHLE N L L F +GL L + LG N +P V + ++ +L L NK+ + + G L+ L L++N ++ + FV+L + L N + +A AF+ +K +L L+L N++ + ++E F G G L L + S L+P LH L+ L N + + + F GL L++L L +I++ + FS + + + ++ N L + P R+ L LHL+ + + +R F L+ L+ L L+ NSI SIE S L+ L LDL NRLT + +F L +LE + L N++ + + L + L + N + L E LFS ++ + L NSL +TF P L+ + L+ N W+CSC
Sbjct: 41 CPVACTCSHDDYTDELSVFCSSKNLTHLPDDIPVSTRALWLDGNNLSSIPSAAFQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYYLHLERNRLRNLAVGLFTHTPSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKL--TYLQPALFCGLGELRELDLSRNALRSVKANVFVHLPRLQKLYLDR--NLITAVAPGAFLGMK-ALRWLDLSHNRV--AGLMEDT---FPGLLGLHVLRLAHNAIAS-----LRPRTFKDLHFLEELQLGHNRI-RQLGERTFEGLGQLEVLTLNDNQITEVRVGAFSGLFNVAVMNLSGNCLRSLPERVFQGLDKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTTNRLTHLPRQLFQG-------------------------LGHLEYLLLSYNQLT--TLSAEVLGPLQRAFWLDISHNHLETLAEGLFSSLGRVRYLSLRNNSL-----QTFSPQ-------------------------------PGLERLWLDANPWDCSC 541
BLAST of Epiphycan vs. UniProt/SwissProt
Match: sp|F7D3V9|LGR5_XENTR (Leucine-rich repeat-containing G-protein coupled receptor 5 OS=Xenopus tropicalis OX=8364 GN=lgr5 PE=2 SV=2) HSP 1 Score: 108.612 bits (270), Expect = 1.571e-22 Identity = 138/484 (28.51%), Postives = 210/484 (43.39%), Query Frame = 2
Query: 185 CPIPCKCTHHS----VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLEN------------------------NDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKL---NLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQ--ILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPH----RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKL 1525
CP PC+C VDC +AL +P+ +SI + LDLS N I +L +N L L+ L+L+ N++ I + AF GL +L+ L L+N N +S + P +FN GL L H++L +N +IPV ++ L L NKI + D +F + L L L N I + + F L TL L N+L+ A A +KN L L N + +I EQA G + L +I F+D+ + H + AF+ L L+ ILN A PD+ + L L + L+ P +LP +L+ L L N+I+ + F L+++ L+ N + I + +F L L SLDL +N++ I F +P LI L+L + LT P + L L ++L GN D ++ PKL
Sbjct: 32 CPTPCECEQEGMLVRVDCSDRALTSLPRNLSIFTSYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLMLQNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFN-GLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHH-IPDYAFRNLSSLVVLHLHNNRIYSLGKKCF--DGLHSLETLDLNYNNLDEFPA-AIKTLKN-LKELGFHSNNI--KSIPEQAFIG-------------NPSLITIH------FYDNPIQH-----------VGRSAFQHLPELRTLILNGASQITEFPDL--TGTTSLESLTLTGAQLVYLPSAVCTQLP-NLQVLDLSYNHIKDL--PSFSGCQRLQKIDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSLPSLIKLDLSSNH---LTSFPVT-GLHGLTHLKLTGNSALQDLIPS---EHFPKL 465 HSP 2 Score: 73.559 bits (179), Expect = 9.929e-12 Identity = 86/297 (28.96%), Postives = 128/297 (43.10%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH---LQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGI----------FHRM-------------PKLISLNLE------------QQNPVLLTIA----------PNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI 1711
+N++L+ + ++A L +LQ L L IS P F+ + L HL+++DN+L P R SL L L N I I F++L++L LHL N I S+ FD L SL +LDL +N L + I FH P LI+++ Q P L T+ P+ ++LES+ L G ++ + +A + LP L L L N I L FSG +Q IDL N + + TF L+ + L NK++ I
Sbjct: 119 QNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFNGLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHHIPDYAFRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAAIKTLKNLKELGFHSNNIKSIPEQAFIGNPSLITIHFYDNPIQHVGRSAFQHLPELRTLILNGASQITEFPDLTGTTSLESLTLTGAQLVYLPSA--VCTQLPNLQVLDLSYNHIKDLPS--FSGCQRLQKIDLRHNEVYEIRSTTFQQLVGLR-SLDLAWNKIAVI 410 HSP 3 Score: 56.6102 bits (135), Expect = 1.596e-6 Identity = 56/189 (29.63%), Postives = 95/189 (50.26%), Query Frame = 2
Query: 1094 THLFMNDNNLINCPHRLPASLKFL---HLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAK 1645
++L ++ NN+ N P + +L FL L N++ I + F L +LK L L+ N + + + + +L SL SL L N ++ + F+ + L L L+ + LT P + LS L+++ L N+I F NL LV L L +N+I L + F G +++ +DL+ N+L +PA
Sbjct: 66 SYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLMLQNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFNGLFSLRHLWLDDNS---LTEIPVRALESLSALQAMTLALNKIHHIPDYA--FRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAA 249
BLAST of Epiphycan vs. UniProt/SwissProt
Match: sp|F1MLX5|LGR4_BOVIN (Leucine-rich repeat-containing G-protein coupled receptor 4 OS=Bos taurus OX=9913 GN=LGR4 PE=3 SV=3) HSP 1 Score: 107.842 bits (268), Expect = 2.892e-22 Identity = 120/425 (28.24%), Postives = 189/425 (44.47%), Query Frame = 2
Query: 185 CPIPCKC-THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-----------------------PETFNKGLIKLLHVYLGNNEFEKIPVLKATHMT---KLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF--------VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-NLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAK-LTHLFMNDNNLINCPHRLPA-SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIES 1345
C PC C VDC + L VP+G+S +LD+S N I L ++F+ L+ L+L+ N++ I+ A GL L+ L L+NN L + PE +GL +L H++L +N ++PV +++ L L NKI S++ D +F + L L L N+IK + Q F ++L L + +L ++ F S+I + +GN LL + + F G + F L S+LH L + SM++ N G L+ L L KIS K L L ++ N++ + P +L+ + LQ N I QI++D F+ LT+LK L L +N I I+ +F L S+T+LD+ FN LT +
Sbjct: 29 CAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQLLDISMNNITQLPEDAFKNFPFLEELRLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSSLQSLRLDANHITSVPEDSFEGLTQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKI-SSIPDFAFTNLSSLVVLHLHNNKIKSLGQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELLFHSNSISVIPDGAFDGNPLLKTIHLYDNPLSFVGNSAFHNL--------------------SELHSLVIRGASMVQRFPN--LTGTVRLESLTLTGTKISSISNNLCQEQKRLRTLDLSYNSIKDLPSFNGCHALEEISLQRNQIHQIKEDTFQGLTSLKILDLSRNLIHEIDDRAFAKLGSITNLDVSFNELTSFPT 430 HSP 2 Score: 73.559 bits (179), Expect = 8.819e-12 Identity = 93/358 (25.98%), Postives = 147/358 (41.06%), Query Frame = 2
Query: 824 TIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLK--PFFDS-KLHHLDLS------------------ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP----HRLP-----------------------ASLKFLHLQSNNIRQIRQDDF-----------------------KSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIK---------------FDATAG-----PIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMN 1621
T V + L+ F Q LD+ +T + D K PF + +L DLS +N+ LK++ ++A RGL +LQ L L I+ P+ F + +L HL+++DN+L P LP +SL LHL +N I+ + Q F K+L +LKEL NSI I +FD L ++ L N L+ + + FH + +L SL + + ++ PN LES+ L G +I D + P F+ L +SL+ NQI ++ E+ F G +++++DLS N
Sbjct: 49 TAVPEGLSAFT-----QLLDISMNNITQLPEDAFKNFPFLEELRLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSSLQSLRLDANHITSVPEDSFEGLTQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIKSLGQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELLFHSNSISVIPDGAFDGNPLLKTIHLYDNPLSFVGNSAFHNLSELHSLVIRGAS--MVQRFPNLTGTVRLESLTLTGTKISSISNNLCQEQKRLRTLDLSYNSIKDLPSFNGCHALEEISLQRNQIHQIKEDTFQGLTSLKILDLSRN 399 HSP 3 Score: 64.6994 bits (156), Expect = 5.262e-9 Identity = 66/230 (28.70%), Postives = 112/230 (48.70%), Query Frame = 2
Query: 956 LDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPH---RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
LD+S N++ + + DAF+ L+ L LA +S P S + +L L + +N L P R +SL+ L L +N+I + +D F+ LT L+ L L NS+ + + +L +L +L L N+++ I F + L+ L+L N + ++ + F L NLE+++L N + A LP L L N I+ + + F G+ ++ I L N L
Sbjct: 62 LDISMNNITQ-LPEDAFKNFPFLEELRLAGNDLSFIHPK-ALSGLKELKVLTLQNNQLKTVPSEAIRGLSSLQSLRLDANHITSVPEDSFEGLTQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHL--HNNKIKSLGQHCFDGLDNLETLDLNYNNLGEFPQA---IKALPSLKELLFHSNSISVIPDGAFDGNPLLKTIHLYDNPL 284 HSP 4 Score: 57.3806 bits (137), Expect = 8.092e-7 Identity = 56/201 (27.86%), Postives = 93/201 (46.27%), Query Frame = 2
Query: 1109 NDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDF---------------------KSLTNLKELH---LKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWP 1639
+ L P L A + L + NNI Q+ +D F K+L+ LKEL L+ N + ++ + + L+SL SL L N +T + F + +L L L+ + + + P S L L+++ L N+I + F NL LV L L +N+I L ++ F G N++ +DL+ N+L +P
Sbjct: 44 SGKGLTAVPEGLSAFTQLLDISMNNITQLPEDAFKNFPFLEELRLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSSLQSLRLDANHITSVPEDSFEGLTQLRHLWLDDNSLTEVPVHPLS-NLPTLQALTLALNKIS--SIPDFAFTNLSSLVVLHLHNNKIKSLGQHCFDGLDNLETLDLNYNNLGEFP 241
BLAST of Epiphycan vs. UniProt/SwissProt
Match: sp|Q9BXB1|LGR4_HUMAN (Leucine-rich repeat-containing G-protein coupled receptor 4 OS=Homo sapiens OX=9606 GN=LGR4 PE=1 SV=2) HSP 1 Score: 104.375 bits (259), Expect = 3.447e-21 Identity = 116/425 (27.29%), Postives = 188/425 (44.24%), Query Frame = 2
Query: 185 CPIPCKC-THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-----------------------PETFNKGLIKLLHVYLGNNEFEKIPVLKATHMT---KLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF--------VXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-NLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPA-SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIES 1345
C PC C VDC + L VP+G+S LD+S N I L ++F+ L+ L+L+ N++ I+ A GL L+ L L+NN L + PE +GL++L H++L +N ++PV +++ L L NKI S++ D +F + L L L N+I+ ++Q F ++L L + +L ++ F S+I + +GN LL + + F G + F L S LH L + SM++ N G +L+ L L KIS P+ L L ++ NN+ + P +L+ + LQ N I QI++ F+ L +L+ L L +N I I + +F L +T+LD+ FN LT +
Sbjct: 29 CAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKI-SSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNL--------------------SDLHSLVIRGASMVQQFPN--LTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDVSFNELTSFPT 430 HSP 2 Score: 80.1073 bits (196), Expect = 9.547e-14 Identity = 82/288 (28.47%), Postives = 125/288 (43.40%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP-HRLP--ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQ------------QNPVLLTIA----PNSFV-------LSNLESIELQGNRI--KFDATAG-----------------------------------------PIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMN 1621
+N+ LK++ ++A RGL LQ L L I+ P+ F + +L HL+++DN+L P H L +L+ L L N I I F +L++L LHL N I S+ + FD L +L +LDL +N L I +P L L NP+L TI P SFV LS+L S+ ++G + +F G P F+ L +SL+ NQI ++ E F G ++++++DLS N
Sbjct: 113 QNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAI-KALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDLSYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRN 399 HSP 3 Score: 63.929 bits (154), Expect = 9.376e-9 Identity = 71/260 (27.31%), Postives = 125/260 (48.08%), Query Frame = 2
Query: 866 GFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPH---RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
G + +D GLT++ + L F + LD+S N++ + + DAF+ L+ L LA +S P S + +L L + +N L P R ++L+ L L +N+I + +D F+ L L+ L L NS+ + + +L +L +L L N+++ I F + L+ L+L N + +++ + F L NLE+++L N + A LP L L N I+ + + F G+ ++ I L N L
Sbjct: 37 GDRRVDCSGKGLTAVP-EGLSAFTQA----LDISMNNITQ-LPEDAFKNFPFLEELQLAGNDLSFIHPK-ALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHL--HNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQA---IKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLYDNPL 284 HSP 4 Score: 55.0694 bits (131), Expect = 4.689e-6 Identity = 55/201 (27.36%), Postives = 94/201 (46.77%), Query Frame = 2
Query: 1109 NDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFK---------------------SLTNLKELH---LKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWP 1639
+ L P L A + L + NNI Q+ +D FK +L+ LKEL L+ N + ++ + + L++L SL L N +T + F + +L L L+ + + + P S L L+++ L N+I + F NL LV L L +N+I L+++ F G N++ +DL+ N+L +P
Sbjct: 44 SGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLS-NLPTLQALTLALNKIS--SIPDFAFTNLSSLVVLHLHNNKIRSLSQHCFDGLDNLETLDLNYNNLGEFP 241
BLAST of Epiphycan vs. TrEMBL
Match: L1I8L9 (Uncharacterized protein OS=Guillardia theta (strain CCMP2712) OX=905079 GN=GUITHDRAFT_121393 PE=4 SV=1) HSP 1 Score: 178.333 bits (451), Expect = 2.834e-41 Identity = 169/563 (30.02%), Postives = 269/563 (47.78%), Query Frame = 2
Query: 197 CKCTHHSVDCRHQALKDVPKGI---SIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVL---KATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTG---------------------FNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGP--IFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLS----TISIDFNSTNYLPIDFAKLKNI 1771
C T SVD + ++ + +G+ + +T + LS N + S+ F L +L+ L LS N++ I E F GL+++R L L N+L+ + PET GL L ++YL NN+ +P + L L SNK+ S V + F L++L L NE+ + +T F L + + L N L +I A F + SL L L NKL +++ E G F+G Q+L L S LTS+ + L L LS N L S+ F GL +LQ L L+ K++ P+ F+ +A L L+++ N L + P + ASL++L+L N + I F LT+L+ L+L N + S+ FD LASL +L L N+LT + + +F+ + L +L L L +I F L++L+++ L N + T+ P +F L L L L N++T + E +F+G ++Q + LS N L P F L +S + L+ N+L+ T+ S YL + KL ++
Sbjct: 64 CTTTCTSVDLSYAGIRRIDEGVFNNTWSLTEIRLSGNKLTSVPATVFAGLASLQYLYLSSNKLTSIPETVFAGLASIRVLILSGNELTSV-PETVFAGLASLQYLYLDNNKLTSVPATVFNGLASLQTLYLSSNKLTS-VPETVFNGLASLRSLYLDNNELTSVPETVFAGLASLQTLYLYD--NELTSIPATVFAGLA-SLQTLYLSYNKL--TSVPETVFDGLASLRSLYLSYNELTSVPETVFDGLASLQYLYLSSNKLTSVPATVFAGL--TSLQTLYLSGNE-LTSVPETVFTGLASLQTLYLSSNKLTSVPETVFNGLASLQTLYLSSNKLTSVPATVFAGLASLQYLYLYDNELTSIPATVFAGLTSLQSLYLSSNKLTSVPETVFDGLASLQTLYLSSNKLTSVPATVFNGLASLQTLYLYDNE--LTSIPATGFNGLASLQTLYLSSNEL----TSIPETVFAGLASLQTLYLSGNELTSVPETVFAGLASLQTLYLSGNELTSVPETVFAGL-ASLQTLYLSSNELTSIPETVFAGLASLQYLYLSSNKLTSV 609 HSP 2 Score: 167.933 bits (424), Expect = 4.530e-38 Identity = 159/514 (30.93%), Postives = 251/514 (48.83%), Query Frame = 2
Query: 212 HSVDCRHQALKDVPKGIS---IDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLN---LDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATAGP--IFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI 1711
S+ + L VP+ + + L LS N + S+ F L +L+ L LS NE+ + E F GL++L+ L+L +N L+ + PET GL L +YL +N+ +P + L L N++ S + F T LQ+L L+ N++ + +T F L TL L N L ++ A F + SL L L N+L TGFNG Q L L S LTSI + L L LS N L S+ F GL +LQ L L+ +++ P+ F+ +A L L+++ N L + P + ASL++L+L SN + + + F L +L+ L+L N + S+ F+ LASL +L L +N+LT + + +F + L SL L LT P + L++L S+ L N + T+ P +F L L L L DN++T + E +F+G ++Q + L N L P F L +S + L+ N+L+++
Sbjct: 285 RSLYLSYNELTSVPETVFDGLASLQYLYLSSNKLTSVPATVFAGLTSLQTLYLSGNELTSVPETVFTGLASLQTLYLSSNKLTSV-PETVFNGLASLQTLYLSSNKLTSVPATVFAGLASLQYLYLYDNELTS-IPATVFAGLTSLQSLYLSSNKLTSVPETVF--DGLASLQTLYLSSNKLTSVPATVFNGLA-SLQTLYLYDNEL-----TSIPATGFNGLASLQTLYLSSNELTSIPETVFAGL--ASLQTLYLSGNE-LTSVPETVFAGLASLQTLYLSGNELTSVPETVFAGLASLQTLYLSSNELTSIPETVFAGLASLQYLYLSSNKLTSVPETVFAGLASLQTLYLSYNELTSVPETVFNGLASLQTLYLSYNKLTSVPATVFAGLASLRSLGLYDNK---LTSVPATVFAGLASLRSLSLDFNEL----TSIPETVFAGLTSLQTLYLYDNELTSVPETVFNGLASLQYLYLDNNKLTSIPETVFAGL-ASVQTLYLSGNELTSV 777 HSP 3 Score: 139.043 bits (349), Expect = 3.097e-29 Identity = 161/574 (28.05%), Postives = 242/574 (42.16%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLN----------------------STIVEQALTG---------------------FNGATGFQFLDLKSCGLTSI----------------SGDFLKPFFD------SKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPA---------------------------------------------------SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPA 1642
L LS N + S+ F L +L+ L LS NE+ + E F GL++L+ L+L N+L+ + PET GL L +YL +NE IP + L L SNK+ S V + F LQTL L+ NE+ + +T F L TL L N L ++ A F + SL +L L NKL + ++I E G FNG Q+L L + LTSI SG+ L + + L +L++S N L S+ F GL +LQ L+L+ K++ P+ F+ +A L L++++N L + P + A SL+ L+L SN + I D F L++L L L N + S+ +F LA LT+L + NRLTR+ G F + L +L+L + LT + L + +++L N++ D A + NL L NLSL DNQ+T L+ + ++ + LS N L PA
Sbjct: 503 LYLSSNELTSIPETVFAGLASLQTLYLSGNELTSVPETVFAGLASLQTLYLSGNELTSV-PETVFAGLASLQTLYLSSNELTSIPETVFAGLASLQYLYLSSNKLTS-VPETVFAGLASLQTLYLSYNELTSVPETVF--NGLASLQTLYLSYNKLTSVPATVFAGLA-SLRSLGLYDNKLTSVPATVFAGLASLRSLSLDFNELTSIPETVFAGLTSLQTLYLYDNELTSVPETVFNGLASLQYLYLDNNKLTSIPETVFAGLASVQTLYLSGNELTSVPETVFNGLASLQYLNVSSNE-LTSVPETVFDGLASLQTLDLSYNKLTSVPETVFAGLASLRSLYLDNNELTSVPETVFAGLDSLWRLDLHSNRLASLALSLFYDCHDLMELYLNNNLLSGLLPGSLDGLVSLEALYLHSNQLADISSDVFAQLSSLTTLTLHNNRLSSLSPGAFAGLARLTTLSIHHNRLTRLSPGAFQGLSTLATLDLHDNHLTSLTAGALTG-LDAMRALDLSSNKLA-DLPAQAL-HNLTGLRNLSLDDNQLTSLSAGVLEPLAGLEYLWLSHNRLAEVPA 1067 HSP 4 Score: 130.183 bits (326), Expect = 1.829e-26 Identity = 139/484 (28.72%), Postives = 225/484 (46.49%), Query Frame = 2
Query: 359 LSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI-SIDFNSTNYLPIDFAKLKNISLENNQ 1789
LS+ ++ I+E F +L + L N L+ + P T GL L ++YL +N+ IP + F ++ LIL+ NE+ + +T F L + + L N L ++ A F + SL L L NKL +++ E T FNG + L L + LTS+ + L L L +N L SI F GL +LQ L L+ K++ P+ F +A L L+++ N L + P + ASL++L+L SN + + F LT+L+ L+L N + S+ F LASL +L L N+LT + +F+ + L +L L LT P + L++L+ + L N + + +F L L +L L N++T + E +F G ++Q + LS N L PA F L +S + L N+L++I + FN A L+ + L +N+
Sbjct: 73 LSYAGIRRIDEGVFNNTWSLTEIRLSGNKLTSV-PATVFAGLASLQYLYLSSNKLTSIP----------------------ETVFAGLASIRVLILSGNELTSVPETVFAGLASLQYLYLDN--NKLTSVPATVFNGLA-SLQTLYLSSNKL--TSVPE---TVFNGLASLRSLYLDNNELTSVPETVFAGL--ASLQTLYLYDNE-LTSIPATVFAGLASLQTLYLSYNKLTSVPETVFDGLASLRSLYLSYNELTSVPETVFDGLASLQYLYLSSNKLTSVPATVFAGLTSLQTLYLSGNELTSVPETVFTGLASLQTLYLSSNKLTSVPETVFNGLASLQTLYLSSNK---LTSVPATVFAGLASLQYLYLYDNELT--SIPATVFAGLTSLQSLYLSSNKLTSVPETVFDGLASLQTLYLSSNKLTSVPATVFNGL-ASLQTLYLYDNELTSIPATGFNG-------LASLQTLYLSSNE 509
BLAST of Epiphycan vs. TrEMBL
Match: A0A1S3ICW5 (chondroadherin-like protein OS=Lingula unguis OX=7574 GN=LOC106163136 PE=3 SV=1) HSP 1 Score: 171.4 bits (433), Expect = 8.708e-40 Identity = 166/636 (26.10%), Postives = 299/636 (47.01%), Query Frame = 2
Query: 116 MKTRFTFCLLLVIYNLVCLYVF---PCPIPCKCT--HHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVL-KATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSI-SGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPD-IRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLT-------------------IAPNSFVL------SNLESIELQGNRIKF---DATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLIS-SAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQI---QWLLHFRKLILNRDQILCNIPNEKAGKNLF 1903
++ + F L+I +VC CP C C + VDCR++ L ++P GI + V L L N + + ++F+ LI+L+ L LS+ ++ +I F +N+ +L+L +N L + + +GL + ++LG NE IP L + + +D+N++ +A + F L + L+ N+I + D +L+ +K+ L L N + I + P+ ++L++L L G+ L+ST + L G + LD+ + + + D + + L L + NS+ ++ F + N ++ + C+I D + F+ MAKL L +N+N L P LP SL+ L L N I + F + N++ L L+K+ + I TNSF L + +LDL NR++ I +F + KL +L L L+ I +S L S L+ + L N + + + G +F NL KL NL+L N+I++L F +++ + LS N + + ++ F P+ S A+++ NM L NS++ ++ +L+ + L + + C+C + +W+ + D CN P E+ G L
Sbjct: 2 VRYKIPFFCALIILAIVCASKSNGQTCPNKCHCKNNYQIVDCRNRGLSEIPSGIPVSVQELRLDHNTLTDIQADAFKGLIHLRVLYLSNCKINKIAPGTFKEPTNITDLYLTSNVLKDFNATSL-QGLQNVQMLHLGGNELTAIPDLGRLRALVSFVIDTNELSNASFPEGFGKLLNLTRITLSNNKITRLADGDLKSLRQSKVAKLYLARNQIAQIGKLSLEPIADTLVSLSL-GHNPLSSTSLRDGLFGLRQSKQLTSLDIPALSFGGVMNSDTFQYLNSTPLLKLKMYCNSV-HQLEPRLFSHVRNADYIDASSCQIRDIEQTVFAGMAKLKELRLNENFLSYVPQNLPNSLRILDLSENQIINFKDFQFAGMRNIETLRLRKSGVERIPTNSFAGLEKIKTLDLSENRISSIGKSVFGMLHKLKTLKLNDNRIGLIQTDIFSSSSALQFLDMSKNYIGRSSIPLDLFKGTSKLKILFLSDNELGYVFKNDPNGMLFKNLYKLENLTLERNRISQLWPAQFQNLTSVKNLSLSDNQVSFFTSQLFAPMTSLRALNLSQNMISL------VNSSSIGGLE-GRLQTLDLSGSPFACTCDLVWFRRWINQTNITLSQLDVYTCNTPAERRGMPLL 627
BLAST of Epiphycan vs. TrEMBL
Match: A0A0D2WX36 (LRRNT domain-containing protein OS=Capsaspora owczarzaki (strain ATCC 30864) OX=595528 GN=CAOG_007756 PE=4 SV=1) HSP 1 Score: 169.474 bits (428), Expect = 4.819e-39 Identity = 177/571 (31.00%), Postives = 264/571 (46.23%), Query Frame = 2
Query: 197 CKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLN---LDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKF---LHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRI-KFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTI-SIDFNSTNYLPIDFAKLKNISLENNQ-WNCSCHQIQWLLHFRKLILNRDQILCNIP 1876
C CT V+C ++L +P GI T L L N I S+S+++F L L ++L N++ + NAF GLS L L L NN LS + F GL L+ + L NN+ +P T +T L L +N+I + ++ +F T LQTL L N+I + F L L L N + + A AF + L L+L N L S I A TG T L L + +T++ + + L +L L N+ + ++ +AF G L L L +I+ P + ++ LT L++ +N + + P + L L L +N I I + F LT L L L N + SI +F L +LT L L N L+ + S F + LI L L N + T+A N+F L+ L ++L GN+I A+A F L LV L L N+IT + N F+G ++ L++LS N + PA F L ++ + L N LS + S F L+ + L NNQ + + L L L R+QI IP
Sbjct: 36 CTCTGTLVNCISRSLTAIPTGIPATTTTLYLQSNQITSISSSAFTGLTALTYMRLDTNQITTVPANAFSGLSTLNTLQLNNNWLSAIPSSAF-TGLTALIQLLLNNNQITTVPSSAFTGLTALQILYLHNNQIATVAIN-AFSGLTALQTLYLYNNQIITVATNAF--SGLAALQVLRLDTNQITTVPANAFSGLSK-LNTLQLNNNWL--SAIPSSAFTGL---TALIQLRLDTNQITTVPANAFSGL--TALIYLYL-YNNQITTVATNAFSGPTALVQLQLYGNQITTIPSSALTGLSALTQLYLYNNQITSVPANGFSGLTALTDLRLSNNTITSILANAFTGLTKLTYLDLSLNQLTSIPAGAFSGLTALTQLLLFNNWLSAVPSSAFTGLTALIYLYLN--NNQITTVAANAFTGLTALVQLQLYGNQITTIPASA---FAGLSALVQLYLYSNRITAIFVNAFTGLTHLSLLELSNNQITSLPANAFAGL-TAMTQLSLYNNSLSAVPSSAFT-------GLTALQALYLYNNQITTVAANAFTGLTALVLLHLYRNQI-TTIP 579 HSP 2 Score: 139.813 bits (351), Expect = 9.404e-30 Identity = 145/504 (28.77%), Postives = 222/504 (44.05%), Query Frame = 2
Query: 239 LKDVPKGISIDVTILDLSF---NLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPV---LKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLN----------------------STIVEQALTG---------------------FNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLK---FLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRI-KFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSG 1585
L VP +T L + N I +++ N+F L L +L+L N++ I +AF GLS L L+L +N ++ + F GL L + L NN+ +P T MT+L+L +N + SAV +F T LQ L L N+I + F L L L N + I A AF + +L+ L L N++ +++ A TG F G T Q L L + ++S++ + + L L L N ++ +I AF GL L +L L +S P F+ + LT L ++ N + P + L +L L N I I F LT L+ L+L N+I +I N+F L +L LDL +++T I + +F +P L LNL N L + ++F L+ L + + GNRI A A F L LV L L+ NQIT ++ + F+G
Sbjct: 407 LSAVPSSAFTGLTALIYLYLNNNQITTVAANAFTGLTALVQLQLYGNQITTIPASAFAGLSALVQLYLYSNRITAIFVNAF-TGLTHLSLLELSNNQITSLPANAFAGLTAMTQLSLYNNSL-SAVPSSAFTGLTALQALYLYNNQITTVAANAFTGLTALVLLHLYR--NQITTIPASAFAGLS-ALVQLYLNSNRITTIFANAFPSLTKLTYLDISNNQITSLPANAFTGLTAMTQLHLYNNLFSTVPSSAFTGLTALQALFLFNNQISSVAANAFTSL--TALIQLQLYGN-LITTIPAGAFSGLSKLNLLQLYNNWLSAIPSSAFTGLTALTQLRLDTNQITTVPANAFSGLTALIYLSLYGNQITTISASAFAGLTALQALYLNDNTITTIAANAFAGLTALNWLDLSDSQITSIPANVFSSLPALAQLNLY--NNWLSAVPTSAFTGLTALTQLTMYGNRITTISANA---FTGLNALVQLFLQSNQITTISASAFTG 897 HSP 3 Score: 128.642 bits (322), Expect = 3.079e-26 Identity = 140/476 (29.41%), Postives = 216/476 (45.38%), Query Frame = 2
Query: 272 VTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPV---LKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIK-FDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTF--LPLIS 1666
+T LDLS N + S+ +F L L +L L +N + + +AF GL+ L L+L NN ++ + F GL L+ + L N+ IP + + +L L SN+I +A+ +F T L L L+ N+I + F L +L NSL + + AF + L+ L N+ I A F G T L L +T+I S L L L+ N + +I +AF L L L+++ +I+ P F+ + +T L + +N P +L+ L L +N I + + F SLT L +L L N I +I +F L+ L L L N L+ I S F + L L L+ + T+ N+F L+ L + L GN+I A+A F L L L L DN IT + N F+G + +DLS + + PA F LP ++
Sbjct: 373 LTYLDLSLNQLTSIPAGAFSGLTALTQLLLFNNWLSAVPSSAFTGLTALIYLYLNNNQITTVAANAF-TGLTALVQLQLYGNQITTIPASAFAGLSALVQLYLYSNRI-TAIFVNAFTGLTHLSLLELSNNQITSLPANAFAGLTAMTQLSLYN--NSLSAVPSSAFTGLT------ALQALYLYNNQITTVAANAFTGLTALVLLHLYRNQITTIPASAFAGL--SALVQLYLNSNRI-TTIFANAFPSLTKLTYLDISNNQITSLPANAFTGLTAMTQLHLYNNLFSTVPSSAFTGLTALQALFLFNNQISSVAANAFTSLTALIQLQLYGNLITTIPAGAFSGLSKLNLLQLYNNWLSAIPSSAFTGLTALTQLRLDTNQ--ITTVPANAFSGLTALIYLSLYGNQITTISASA---FAGLTALQALYLNDNTITTIAANAFAGLTALNWLDLSDSQITSIPANVFSSLPALA 830 HSP 4 Score: 89.7373 bits (221), Expect = 2.900e-14 Identity = 104/359 (28.97%), Postives = 152/359 (42.34%), Query Frame = 2
Query: 272 VTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDS--NKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRI 1339
+T LD+S N I SL N+F L + +L L +N + +AF GL+ L+ L L NN +S + F L L+ + L N IP + ++KLNL N SA+ +F T L L L N+I + A AF + +LI L L GN++ +TI A F G T Q L L +T+I+ + + L+ LDLS+ S + SI + F L L LNL +S P F+ + LT L M N I I + F L L +L L+ N I +I ++F + LT L L N++T +
Sbjct: 613 LTYLDISNNQITSLPANAFTGLTAMTQLHLYNNLFSTVPSSAFTGLTALQALFLFNNQISSVAANAFTS-LTALIQLQLYGNLITTIPAGAFSGLSKLNLLQLYNNWLSAIPSSAFTGLTALTQLRLDTNQITT--------------------------VPANAFSGLT-ALIYLSLYGNQI--TTISASA---FAGLTALQALYLNDNTITTIAANAFAGL--TALNWLDLSD-SQITSIPANVFSSLPALAQLNLYNNWLSAVPTSAFTGLTALTQLTM---------------------YGNRITTISANAFTGLNALVQLFLQSNQITTISASAFTGPSLLTQLYLSNNQITTM 914
BLAST of Epiphycan vs. TrEMBL
Match: A0A2H4HHW4 (Toll-like recptor 11 OS=Oncomelania hupensis OX=56141 GN=TLR11 PE=2 SV=1) HSP 1 Score: 163.31 bits (412), Expect = 4.954e-37 Identity = 180/639 (28.17%), Postives = 294/639 (46.01%), Query Frame = 2
Query: 185 CPIPCKC--THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNK-GLIKLLHVYLGNNEFEKIPVLKA-THMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLS----ENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLES---IELQGNR---IKFDATAGPIFDNLPKLVNLSLRDNQITKL-----NENLFSGSVNIQL------------------------IDLSMNSLIGWPAKTFL---PLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKL-------ILNRDQILCNIPNEKAGKNLFLV---MVNCSD 1927
CP C+C SV+C + L D+P+GI V L L N L + + L ++ L +++ ++ AF LS L+ L+L NN++ L PE F K G + LL+ L N +P L +T L L++N+I++ + + L T++L+ N + E+ F L N L L + ++++ + A A P+ L +L++ G ++ + L G + +G LD++ + + G F S L H L N+ + +I AF L L L+L CKIS DP FS + L LF+N+N L P LP+SL L+LQ N++ ++ DF +L L+EL+L + I + +SF L +L L L N+L+ I +F PKL LNL N + ++ PN +++L S +++ N I FD F +L L NL L N++ L + L SG VN+Q I+L N + W K F L + + L++N ++ + N T+ + F ++ N+S N + C+C ++W FR + N+ + CN P E K L+ +NC+D
Sbjct: 96 CPAKCQCGKAGTSVNCVGKGLTDIPEGIPSQVETLYLDRNNFGVLKPGALENFQFLSKVYLRSCKIQTVSPGAFHNLSLLQELYLGNNNIDQLQPEAFQKAGALYLLN--LQANRLTSVPDLHTLPGLTNLVLEANRIQNGTFPEGYSSLQKLSTVVLSNNNLTELTNGTFTTLANCSLRRLEISRSNIKRVEAGALTPLV-MLQSLKI-GYNPIDGEQLHNLLEGLSADSGLVSLDIR---MIDLRGSLPSVSF-SILAHTPLITLHFRNNEVGTIPAKAFANLPKLTTLDLQICKISVVDPQA-FSDLPALQLLFLNNNALTTVPKTLPSSLTKLYLQFNSLSALKGGDFSNLGKLQELYLSTSGITEVNEDSFLGLNNLVILHLNQNKLSNIPKKLFSYTPKLRILNLRNNN--IQSLKPNPGAMASLTSLLTLDMSHNNCGYIPFD-----YFTDLTSLENLDLSYNRLGGLMAGDVSGRLLSGMVNLQRLSLESNNINFIYDAQFQNLHKLANINLKNNRISSWGPKLFYTPGTLRKTLKTVDLSINTIALV----NETSVRDLQFVQMLNLS--TNPFACTC-DLRW---FRAWLENTTINVANKTKYTCNSPAEWEHKLLWTFGADKINCTD 708
BLAST of Epiphycan vs. TrEMBL
Match: A0A2I7NB23 (Tool like receptor 3 OS=Octopus vulgaris OX=6645 PE=2 SV=1) HSP 1 Score: 160.614 bits (405), Expect = 1.785e-36 Identity = 170/640 (26.56%), Postives = 299/640 (46.72%), Query Frame = 2
Query: 164 VCLYVFPCPIPCKCTHHS--VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKA-THMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSI---SGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKF------------------------LHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRI-KFDAT--AGPIFDNLPKLVNLSLRDNQITK-LNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKL----ILNRDQILCNIPNEKAGKNLF---LVMVNCSDVILSRILS 1951
+C+ CP C+C V C+ Q+L VPK + +L L +NL++ ++ SF+ L+ LK L L+ N++ I +N+F S L +L+L NN + + +F G I L +YL +N+ + +P L + L +++N+I + L + L+ N+I + +NL + + ++ N + NI E F + N + +L L N + +++ +L F L GL I + + + ++ L L LS +S + + ++ F+ LD+L++L L C+I+ + F+ + L LF+N N + P+ L + LHLQ N I Q +D F L NLK L L+ N I I F LA L+ L LG N +T I IF R+ L L+L + ++ P SF L+ + + L N++ KF +T G + L L + L N+I L++ +F + +++ I N + GW TF +++ + L+++ I+ D +S Y I+ K NI+L N + C C+ ++W + I++++ ++CN P + GK L ++C+D L IL+
Sbjct: 14 ICIVNTTCPESCQCNKEQTKVSCKRQSLTSVPKSLPTTTEVLSLEYNLLREITAESFKNLVKLKELVLTSNKISVIRKNSFKYQSKLVSLYLINNIIHTIENGSF-VGAISLASIYLNSNQLKDVPCLYGLISLKHLLINTNQISEVFFPAEYRQIKNLTYINLSNNKINRLTVNSLMNLNESNIKKISFSRNQITNIEPETFRVLSN-INSLNLAENP-FTADVLKNSLYAMR---NFNLRSLDLSGLKKINVLADNIFEALQNTSLQTLILS-HSNISRVSSNTFKLLDSLEVLKLDFCQIASLEESTFTNLTHLRRLFLNGNYISKLTAVFPSKLLYLYLNSNKIQKVPSKVFDELFHLIELHLQYNKIVQFDKDAFIGLENLKTLKLQHNHINVIPGGLFSTLAGLSDLQLGSNDITTIPEEIFIRLKSLSFLDLSNNH---ISKMPKSFSKELTRIRKLYLDSNKLGKFLSTDKKGFLLSGLKNLERIDLSYNEIQGHLSKKIFQNNKHLKYIYFRGNKITGWENNTF--EVTNLTLMELDVSNNFIITFDSDSLKY--INRKKKFNITL--NPFACDCN-LRWFRDWLNTTTVDIVDKNDLMCNSPPDWQGKKLLDFSRSKIDCTDYTLYYILA 636
BLAST of Epiphycan vs. Ensembl Cavefish
Match: igfals (insulin like growth factor binding protein acid labile subunit [Source:NCBI gene;Acc:103036545]) HSP 1 Score: 107.842 bits (268), Expect = 1.481e-23 Identity = 142/514 (27.63%), Postives = 223/514 (43.39%), Query Frame = 2
Query: 188 PIPC--KCT--------HHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKN--------------EIKEI----NQTDFVXXXXXXXXXXXXXM----NSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLP-------ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPK----LISLNLEQQNP--VLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSG 1585
PIPC +CT SV C + L P + L L NL +L +SF+ L NL L L ++ ++ F L NL +LHLE N L +L P + L + L NN+ +I +HM LNL N + S + + F GL+ L+L+ N E+KE+ NQ + K+ N + +A AFV +K SL L+L N+L TI+ F G L L + + S+ + L LD+ N + +++ F GL +L++L+L K+ D R L+HL + +C LP + L +HL + ++ F L +L+ L L+ N+I +++ SF +L L LD+ FN L + S F+ + L+S NL + P VLL L L ++L GN+++ A LP+L L+L+DN +T L ++ +G
Sbjct: 30 PIPCSKRCTCLLDDYTSELSVYCSSRNLTQAPPDLPASTRSLWLDGNLFATLPADSFKNLTNLDFLNLQSGQLTSLDSQVFKRLHNLAHLHLERNSLRIL-PAAIFQNTPNLATLNLHNNQLSRIEERLFAGLSHMWLLNLGWNSL-SVLPETGFHDLHGLRELVLSGNRLAYLQPQLFQGLTELKELDLSGNQLRVIKANVFLKLPKLQKLYLAQNQITTVAPRAFVGMK-SLRWLDLSRNRL---TILHDE--TFLGLHSLHVLRLSNNSINSLRPGTFRDL--QYLEELDMCHNQI-RALGERVFEGLGHLEVLSLEYNKLQD--ARSGTFMGLSHLAVIKLT-GSCFRSLPDQVFKGLSKLHSMHLDKGCLTKVSTQGFIGLASLRRLFLQHNNISAVDRQSFVELQGLQQLDMRFNNLASLTSHTFYGLKALEYLLLSKNLIRHFPSEVLLP-------LQQLSWLDLSGNKLEVLINA--TLYMLPRLRYLNLKDNFLTTLPSSVPNG 520 HSP 2 Score: 73.9442 bits (180), Expect = 5.370e-13 Identity = 82/295 (27.80%), Postives = 129/295 (43.73%), Query Frame = 2
Query: 851 FNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSM-----------------------LKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTF 1651
F T FL+L+S LTS+ K + L HL L NS+ L I+ F GL ++ +LNL +S P+ F + L L ++ N L +L LK L L N +R I+ + F L L++L+L +N I ++ +F + SL LDL NRLT + F + L L L N + ++ P +F L LE +++ N+I+ A +F+ L L LSL N++ F G ++ +I L+ + P + F
Sbjct: 86 FKNLTNLDFLNLQSGQLTSLDSQVFKRLHN--LAHLHLERNSLRILPAAIFQNTPNLATLNLHNNQLSRIEERLFAGLSHMWLLNLGWNSLSVLPETGFHDLHGLRELVLSGNRLAYLQPQLFQGLTELKELDLSGNQLRVIKANVFLKLPKLQKLYLAQNQITTVAPRAFVGMKSLRWLDLSRNRLTILHDETFLGLHSLHVLRLS--NNSINSLRPGTFRDLQYLEELDMCHNQIR--ALGERVFEGLGHLEVLSLEYNKLQDARSGTFMGLSHLAVIKLTGSCFRSLPDQVF 374 HSP 3 Score: 70.4774 bits (171), Expect = 6.570e-12 Identity = 65/206 (31.55%), Postives = 97/206 (47.09%), Query Frame = 2
Query: 1100 LFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTIS 1714
++ + NL P LPAS + L L N + D FK+LTNL L+L+ + S+++ F L +L L L N L + + IF P L +LNL N L I F LS++ + L N + G F +L L L L N++ L LF G ++ +DLS N L A FL L + L N+++T++
Sbjct: 50 VYCSSRNLTQAPPDLPASTRSLWLDGNLFATLPADSFKNLTNLDFLNLQSGQLTSLDSQVFKRLHNLAHLHLERNSLRILPAAIFQNTPNLATLNL--HNNQLSRIEERLFAGLSHMWLLNLGWNSLSVLPETG--FHDLHGLRELVLSGNRLAYLQPQLFQGLTELKELDLSGNQLRVIKANVFLKL-PKLQKLYLAQNQITTVA 250
BLAST of Epiphycan vs. Ensembl Cavefish
Match: ENSAMXT00000052087.1 (toll-like receptor 13 [Source:NCBI gene;Acc:103042013]) HSP 1 Score: 106.686 bits (265), Expect = 7.677e-23 Identity = 175/706 (24.79%), Postives = 287/706 (40.65%), Query Frame = 2
Query: 206 THHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI---PVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLE--NIAAEAF-------VPVKNSLINLELEG-------NKL------LNSTIVEQALTGFN-----------GATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLP--ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDD------------------------LASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNEN------------------------------------------LFSGSVNI--------------QLIDLSMNSLIGWPAKTFLPL--------ISSAID-----IRLNMNKLSTISIDFNSTNYLPID----FAKLKNISLENNQWNCSCHQ---IQWLLHFRKL-ILNRDQILCNIPNEKAGKNL 1900
T+ V C + L+ VP I LD+S+N I + N + L NL+ L +S N + E+ + AF GL+ L L+LE N L + F + L L + L N +I L + LNL N + S + L +Q L + KN V+ + +L L L N L+ I A+ F + + N+ I E++ N+L L + ++ L FN G + L +C +TS++ L L N + SI + + NL +L+LA+ +++ + F +M KL L + N L P + +SLK L L N+I + D +LT L LH+ +N + S+E +F D L SL LD N+L+ I+ GIF + L +LE Q+ + + +F L NL ++LQ N++ + + IF LP L L L +N+I+ +++ L++G++NI L+D S N+LI A F PL I + I I+ N+N++ + + NS + + F+KL ++ L+ N+++C C I W +H +L+ D+ CN P+ G L
Sbjct: 53 TNLKVLCYKRQLQAVPDSIPSRSRALDISYNKISLIKRNDLRDLSNLQHLNISKNMIVEVEDGAFRGLAALEELNLEFNKLKTISDGLF-QNLFNLTFLRLNENRISEISSSAFLPLRSLVMLNLSGNHLHSIHKAQPAFLMPNIQELHIGKNGFASFESLQ-VSNTSIQLKALDLSHNPLQLFRITADIFPHLETLDLALINTSIKWEVQNKDYFRNVNRLNISGIQLATEETKELLRTFNSTLDNLRLYYFGEEKAKSLVKDACLITSLTS-------------LRLESNG-INSISENELKHCKNLILLDLAENHLTNISPLTFKSMGKLNTLSLTHNKLKKVPKAIRNLSSLKVLDLGYNSITTVACSDLTNLTGLITLHIYRNPLSSVEHCAFQDLQNLKVLLIGSARLFTLKGYFMRGLQSLQRLDTSHNKLSTIQKGIFKGLLNLT--HLEFQDNQIQILEEGAFEGLDNLIILDLQSNKLTKSSISASIFSGLPNLKELVLNNNRISYTSQSSLSVPPFKNLNSLHSLIIFSQGTDGMKYFPSNLLEGLANLKKLWAGNININSLHPETFTYTPNLWLVDFSKNNLISISADIFQPLKKLSALTMIQTGIQSLDFVIQANINEVQVLLVRKNSIAVINENLINAFSKLSHLDLQGNEFSCDCDNAWFIDWAVHNNDTQVLSLDKFTCNYPSNLRGSRL 740
BLAST of Epiphycan vs. Ensembl Cavefish
Match: ENSAMXT00000025596.2 (pep primary_assembly:Astyanax_mexicanus-2.0:1:16543202:16552825:-1 gene:ENSAMXG00000024870.2 transcript:ENSAMXT00000025596.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 100.523 bits (249), Expect = 4.538e-21 Identity = 162/581 (27.88%), Postives = 251/581 (43.20%), Query Frame = 2
Query: 104 LKNFMKTR---FTFCLLLVIYNLVCLYVFPCPIPCKCTHHS--VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKE--INENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFD-SKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH---LQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPI----DFAKLKNISLENNQ 1789
++ +MK R T LL + + C CKC ++ + C ++ L + P+ + L ++ NLI++L + L+ L L++N + E +S+L L L N +S L F + L L + L N + +P + T++ L L N+I S +F + L+ + L KN IK I + F N L+ N+L ++ F+ + + L +L+L N+L ST+ L T L L G+T + + F+ S L L+L+ N LK I A RGL L L+L+ K+ P F + KL HL + D L + P L +L L L N I I D F L+ LK LH+ N I + +++F L L +DL N+LT I SG+F KL L+L + +LT P F +P+L L L+ N I+ + EN FSG ++ +DLS N PL D + L+ + + FN LP D KLKN++L NN+
Sbjct: 18 VQKYMKLRSKCGTMLGLLSVVMTLTAAAGACIKSCKCVDNATIITCSNKNLLEFPQ-LPPGTKNLYVTHNLIRALPREGLEELL---VLDLTNNRLSEWLTGTTTLPNMSSLTELFLRGNGISSLKTGKFQE-LTNLTLLDLSENNIKILPQKFLQGLTNLKILVLSLNEILSLSYG-AFEGGSDLEEIHLNKNHIKIIEKGVFENCTYLNKLVLSN--NNLSSVEEGTFLGL-HGLRHLDLSKNRL--STVPTGTLKDTPNLTS---LYLHQNGITRVPEN---AFYSLSSLQMLNLNNNP-LKVIHAAALRGLRKLHDLDLSSSKLQVLPPSVFQDVGKLKHLCLFDIALTSLPQGLFNNLTLLQELQLDKNKIHIIPPDLFHPLSKLKHLHMDSNLISELNSSTFRTLRRLVHIDLSNNQLTNISSGLFKGTRKLAHLDLSKN---MLTAVPTE------------------------AFKEIPRLNRLFLQKNAISSVPENAFSGLAGLKKLDLSNN-----------PLEILDEDSLRGLFMLAELDLSFNQLQDLPSCVFQDLGKLKNLNLYNNE 542 HSP 2 Score: 99.7525 bits (247), Expect = 8.184e-21 Identity = 153/539 (28.39%), Postives = 236/539 (43.78%), Query Frame = 2
Query: 218 VDCRHQALKDVPKGISID---VTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMT---KLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIF----------------HRMPKLISLNLEQQNPVLL------------------------------TIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLIS 1666
+D L VP G D +T L L N I + N+F +L +L+ L L++N +K I+ A GL L +L L ++ L VL P F + + KL H+ L + +P ++T +L LD NKI D L + L+ L + N I E+N + F L+ L+ N L NI++ F + L +L+L N L + + +A F L L+ ++S+ + + L LDLS N L+ +D D+ RGL L L+L+ ++ D P F + KL +L + +N L P RL LK L L SNNI I + F LT L++L L N+IL + + F +L L SL L N L+RI +F H +PKL +L+ + L ++ F L NL +++L N + + + F +L L + L N++ L ++FS N+Q + L N L P F L S
Sbjct: 248 LDLSKNRLSTVPTGTLKDTPNLTSLYLHQNGITRVPENAFYSLSSLQMLNLNNNPLKVIHAAALRGLRKLHDLDLSSSKLQVLPPSVF-QDVGKLKHLCLFDIALTSLPQGLFNNLTLLQELQLDKNKIHIIPPDLFHPL-SKLKHLHMDSNLISELNSSTFRTLRRLVHIDLSN--NQLTNISSGLFKGTR-KLAHLDLSKNML--TAVPTEA---FKEIPRLNRLFLQKNAISSVPENAFSGL--AGLKKLDLSNNP-LEILDEDSLRGLFMLAELDLSFNQLQDLPSCVFQDLGKLKNLNLYNNELDTIPERLFNNLTQLKVLQLDSNNISVIFPETFNPLTELRDLQLDNNNILQLNASLFKNLKHLHSLYLNNNALSRIPKYLFQTTRHLKELQLTNNHIHNIPKLSFKSLKMLRALRLNDNHLSDLHKELLAGLDDLRELSLNRNRIESLPVEFFTLPNLRTLDLSNNNL--NTLSSEDFKHLTTLREIHLSFNKLKALPHSIFSSQGNLQKLFLQNNYLSHLPLNVFYKLNS 771 HSP 3 Score: 87.8113 bits (216), Expect = 4.701e-17 Identity = 112/358 (31.28%), Postives = 171/358 (47.77%), Query Frame = 2
Query: 281 LDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPV-----LKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP----HRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFN 1324
LDLSFN ++ L + FQ L LK L L +NE+ I E F L+ L+ L L++N++SV+ PETFN L +L + L NN ++ LK H LN N S + F L+ L LT N I I + F SL+ + A L L N L S + ++ L G + + L L + S+ +F F L LDLS N+ L ++ ++ F+ L L+ ++L+ K+ P FS+ L LF+ +N L + P ++L SLK L L++N++ + F SL NL++LHLK N + S+ +F+ L SL ++ L N
Sbjct: 512 LDLSFNQLQDLPSCVFQDLGKLKNLNLYNNELDTIPERLFNNLTQLKVLQLDSNNISVIFPETFNP-LTELRDLQLDNNNILQLNASLFKNLKHLHSLYLN---NNALSRIPKYLFQTTRHLKELQLTNNHIHNIPKLSF---------------KSLKMLRA------------LRLNDNHL--SDLHKELLAGLD---DLRELSLNRNRIESLPVEF---FTLPNLRTLDLSNNN-LNTLSSEDFKHLTTLREIHLSFNKLKALPHSIFSSQGNLQKLFLQNNYLSHLPLNVFYKL-NSLKQLDLENNHLHHLANKQFHSLKNLEQLHLKSNRLRSLSNGTFEALTSLRAIYLSEN 828 HSP 4 Score: 86.2705 bits (212), Expect = 1.342e-16 Identity = 121/456 (26.54%), Postives = 207/456 (45.39%), Query Frame = 2
Query: 218 VDCRHQALKDVPKGI---SIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKL---NLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLS-ENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRF-SAMAKLTHLFMNDNNLINCP---HRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDN 1549
+D + L ++ G+ + + LDLS N++ ++ +F+ + L RL L N + + ENAF GL+ L+ L L NN L +L ++ +GL L + L N+ + +P + KL NL +N++ + ++ F T L+ L L N I I E F P+ EL +L N+ I++ + F L L + L+ I K F + H +L N+ + +I +F+ L L+ L L +SD + + L L +N N + + P LP +L+ L L +NN+ + +DFK LT L+E+HL N + ++ + F +L L L N L+ + +F+++ L L+LE N L +A F L NLE + L+ NR++ + + F+ L L + L +N
Sbjct: 416 IDLSNNQLTNISSGLFKGTRKLAHLDLSKNMLTAVPTEAFKEIPRLNRLFLQKNAISSVPENAFSGLAGLKKLDLSNNPLEILDEDSL-RGLFMLAELDLSFNQLQDLPSCVFQDLGKLKNLNLYNNEL-DTIPERLFNNLTQLKVLQLDSNNISVI--------------------------FPETFNPLT------ELRDLQLDNNNILQLNASLFKNLKHLHSLYLNNNALSRIP----KYLFQTTRHLKELQLTNNHIHNIPKLSFKSLKMLRALRLNDNHLSDLHKELLAGLDDLRELSLNRNRIESLPVEFFTLP-NLRTLDLSNNNLNTLSSEDFKHLTTLREIHLSFNKLKALPHSIFSSQGNLQKLFLQNNYLSHLPLNVFYKLNSLKQLDLE--NNHLHHLANKQFHSLKNLEQLHLKSNRLR--SLSNGTFEALTSLRAIYLSEN 828
BLAST of Epiphycan vs. Ensembl Cavefish
Match: tlr3 (toll-like receptor 3 [Source:ZFIN;Acc:ZDB-GENE-040219-7]) HSP 1 Score: 98.2117 bits (243), Expect = 2.657e-20 Identity = 167/699 (23.89%), Postives = 291/699 (41.63%), Query Frame = 2
Query: 161 LVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-PETFNKGLIKLLHVYLGNNEF----EKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSL--ENIAAEAFVPVKNSLINLELEG---NKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSM-----------------------LKSIDNDAFRGLDNLQILNL----AKCKISDP---DIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH-----LQSNNIRQIRQDDFKSL--TNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESG----------------------IFHR----MPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSG--------SVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKL----STISIDFNSTNYL---------------PIDFAKLKNISLENNQWNCSCHQIQWLLHF-----RKLILNRDQILCNIPNEKAGKNLFLVMVN-CSDVIL 1936
++C FP C DC H L +VP G+ D++ LD+S N +K L + +L+ L +S+N + + L L++L ++ N VLH T + +L H+ L +N E VLK+ +T L++ NK+ +A + L + L TLIL+ N+ + +TD + ++ + L ++SL + + F PV ++L +L L+G N LL E + T + L L+S T + K + L LDLS N + +K + D F GL ++ LNL K K + P D F + KL HLFM D + + + A L L ++ I F SL + L+ L+L + ++ ++ +F L +LT+L L +N +++ +G + HR +P L L L + L + P+ F L+NL ++L N I + +G + + L L L ++ N + +L ++ G + N+ +++L N L P K L ++ L+ N L +I D + YL + + L + +E+N ++C+C IQW + + +CN P +++ N C D++L
Sbjct: 14 VLCCDSFPHKTTCTIKEGKADCSHMNLNNVPPGLPPDISTLDMSHNRLKVLDPGALSRYPSLEHLDVSYNSIMGLGPGLCDALPRLQHLSVQRNQ--VLHLTATELRNCSRLTHLDLSDNRLKLIGEPFSVLKS--LTWLDVSRNKLITAGLGTKPQLPS-LVTLILSGNDFTTL-KTDDFSFLSSSPSFRVLGLSSLPLKKVEHGCFKPV-SALTDLVLDGCNLNTLLTKVCDELS------DTSVRNLTLRSTLQTVLKNITFKGLLKTNLTMLDLSSNKISKIDDGTFQWLPLLESLSLENNNIKHLTKDTFMGLKSVTQLNLQKALVKTKAAYPIIDDFSFQPLVKLEHLFMRDTSFRDITENMFAGLPSLRTLDLSWSKAGLKTISNTTFASLQGSPLQVLNLTRMALTKLDPGAFSCLGNLTTLVLKYNFISQDLTGEEFRGLNSIREIYLSLNQQKISLTHRSFIHVPTLRVLMLGRALTGTLDMEPSPFRPLTNLTVLDLSNNNIA-NFNSG-LLEGLYHLKVLKMQHNNLARLWKDANPGGPVLFLKDTQNLSVLELDYNGLDEIPLKALRGLFHLN-ELSLSGNLLNYMHGSIFNDLQALRYLRMEKNLLTSVLMGTFSVPLSNLSELHMEHNPFDCTCESIQWFSDWLNSTNTSVPERTSSYICNTPAAYFNRSVLDFQTNSCKDLLL 696
BLAST of Epiphycan vs. Ensembl Cavefish
Match: tlr3 (toll-like receptor 3 [Source:ZFIN;Acc:ZDB-GENE-040219-7]) HSP 1 Score: 95.9005 bits (237), Expect = 1.113e-19 Identity = 166/693 (23.95%), Postives = 288/693 (41.56%), Query Frame = 2
Query: 179 FPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-PETFNKGLIKLLHVYLGNNEF----EKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSL--ENIAAEAFVPVKNSLINLELEG---NKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSM-----------------------LKSIDNDAFRGLDNLQILNLAKC----KISDP---DIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLH-----LQSNNIRQIRQDDFKSL--TNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESG----------------------IFHR----MPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSG--------SVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKL----STISIDFNSTNYL---------------PIDFAKLKNISLENNQWNCSCHQIQWLLHF-----RKLILNRDQILCNIPNEKAGKNLFLVMVN-CSDVIL 1936
FP C DC H L +VP G+ D++ LD+S N +K L + +L+ L +S+N + + L L++L ++ N VLH T + +L H+ L +N E VLK+ +T L++ NK+ +A + L + L TLIL+ N+ + +TD + ++ + L ++SL + + F PV ++L +L L+G N LL E + T + L L+S T + K + L LDLS N + +K + D F GL ++ LNL K K + P D F + KL HLFM D + + + A L L ++ I F SL + L+ L+L + ++ ++ +F L +LT+L L +N +++ +G + HR +P L L L + L + P+ F L+NL ++L N I + +G + + L L L ++ N + +L ++ G + N+ +++L N L P K L ++ L+ N L +I D + YL + + L + +E+N ++C+C IQW + + +CN P +++ N C D++L
Sbjct: 25 FPHKTTCTIKEGKADCSHMNLNNVPPGLPPDISTLDMSHNRLKVLDPGALSRYPSLEHLDVSYNSIMGLGPGLCDALPRLQHLSVQRNQ--VLHLTATELRNCSRLTHLDLSDNRLKLIGEPFSVLKS--LTWLDVSRNKLITAGLGTKPQLPS-LVTLILSGNDFTTL-KTDDFSFLSSSPSFRVLGLSSLPLKKVEHGCFKPV-SALTDLVLDGCNLNTLLTKVCDELS------DTSVRNLTLRSTLQTVLKNITFKGLLKTNLTMLDLSSNKISKIDDGTFQWLPLLESLSLENNNIKHLTKDTFMGLKSVTQLNLQKALVKTKAAYPIIDDFSFQPLVKLEHLFMRDTSFRDITENMFAGLPSLRTLDLSWSKAGLKTISNTTFASLQGSPLQVLNLTRMALTKLDPGAFSCLGNLTTLVLKYNFISQDLTGEEFRGLNSIREIYLSLNQQKISLTHRSFIHVPTLRVLMLGRALTGTLDMEPSPFRPLTNLTVLDLSNNNIA-NFNSG-LLEGLYHLKVLKMQHNNLARLWKDANPGGPVLFLKDTQNLSVLELDYNGLDEIPLKALRGLFHLN-ELSLSGNLLNYMHGSIFNDLQALRYLRMEKNLLTSVLMGTFSVPLSNLSELHMEHNPFDCTCESIQWFSDWLNSTNTSVPERTSSYICNTPAAYFNRSVLDFQTNSCKDLLL 701
BLAST of Epiphycan vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005066.1 (pep scaffold:Pmarinus_7.0:GL476584:229611:317338:-1 gene:ENSPMAG00000004069.1 transcript:ENSPMAT00000005066.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 90.1225 bits (222), Expect = 1.927e-20 Identity = 70/231 (30.30%), Postives = 104/231 (45.02%), Query Frame = 2
Query: 1133 PHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPID-FAKLKNISLENNQWNCSCHQIQWL 1822
P +P + ++L+L N I ++ F SL L E L+ + S+ T FD L LT L+L N+L + G+F R L NL+ + L GN++ A + D+L +L L+LR+NQ+T L E +F VN+Q + L +N L PA F D N+ +L S S D L +I L NN W+C+C I +L
Sbjct: 24 PAGIPTTTQYLYLLVNQITKLEPGVFHSLAALVETRLRSEELASLPTGVFDKLTQLTRLELQTNQLKGVPRGLFDR-------------------------LVNLQQLYLGGNQL--SALPDGVLDSLTQLTYLTLRNNQLTALPEGVFDRLVNLQQLYLHLNRLSSIPAGMF--------DKLTNLKELKLYSNQLKSIPRGAFDNLKSLTHIWLLNNPWDCACTDIMYL 219 HSP 2 Score: 68.9366 bits (167), Expect = 3.568e-13 Identity = 53/169 (31.36%), Postives = 77/169 (45.56%), Query Frame = 2
Query: 188 PIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP--VLKA-THMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF 685
P C C+ SVDCR + VP GI L L N I L F +L L +L E+ + F L+ L L L+ N L + F++ L+ L +YLG N+ +P VL + T +T L L +N++ +A+ + F LQ L L N + I F
Sbjct: 3 PSQCSCSGTSVDCRSRRHASVPAGIPTTTQYLYLLVNQITKLEPGVFHSLAALVETRLRSEELASLPTGVFDKLTQLTRLELQTNQLKGVPRGLFDR-LVNLQQLYLGGNQLSALPDGVLDSLTQLTYLTLRNNQL-TALPEGVFDRLVNLQQLYLHLNRLSSIPAGMF 169 HSP 3 Score: 58.151 bits (139), Expect = 1.746e-9 Identity = 50/134 (37.31%), Postives = 70/134 (52.24%), Query Frame = 2
Query: 935 FD--SKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPH----RLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDL 1315
FD ++L L+L N LK + F L NLQ L L ++S PD ++ +LT+L + +N L P RL +L+ L+L N + I F LTNLKEL L N + SI +FD+L SLT + L
Sbjct: 73 FDKLTQLTRLELQTNQ-LKGVPRGLFDRLVNLQQLYLGGNQLSALPDGVLDSLTQLTYLTLRNNQLTALPEGVFDRL-VNLQQLYLHLNRLSSIPAGMFDKLTNLKELKLYSNQLKSIPRGAFDNLKSLTHIWL 204 HSP 4 Score: 48.9062 bits (115), Expect = 1.848e-6 Identity = 35/112 (31.25%), Postives = 53/112 (47.32%), Query Frame = 2
Query: 1061 PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNL 1387
P F + +LT L + N L P L +L+ L+L N + + SLT L L L+ N + ++ FD L +L L L NRL+ I +G+F ++ L L L
Sbjct: 69 PTGVFDKLTQLTRLELQTNQLKGVPRGLFDRLVNLQQLYLGGNQLSALPDGVLDSLTQLTYLTLRNNQLTALPEGVFDRLVNLQQLYLHLNRLSSIPAGMFDKLTNLKELKL 180
BLAST of Epiphycan vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010345.1 (pep scaffold:Pmarinus_7.0:GL477728:405586:411287:1 gene:ENSPMAG00000009370.1 transcript:ENSPMAT00000010345.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 81.6481 bits (200), Expect = 9.530e-17 Identity = 81/289 (28.03%), Postives = 136/289 (47.06%), Query Frame = 2
Query: 968 ENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV-LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCH---QIQWL 1822
N+ + +I +AF+ L++L+ ++L IS D R A AK SL+ ++L NNIR ++ F+ + +LK L L KNSILS +++F L+ L+ LD+ N +T + + F P L+ L+L + I+P +FV L NL+ + L N+I A +L L L L +N I ++ + F G N++ + S N+++ K + ++ NK+ I ST + L+ I L N ++C C +QWL
Sbjct: 67 ANNEITNIGANAFQNLNHLKNIHLESNHISGFDSRAFAGAK--------------------SLQMINLYGNNIRSLQSKAFQGVPSLKYLILGKNSILSFTSSTFQGLSELSDLDMPMNNITSLPALAFQHTPSLVILDLAYNK--IQQISPKAFVGLKNLDYLNLDNNKIT--TIAPGTLQSLQNLEMLVLNNNNIKRVTSSTFKGLTNLRELYFS-NNMVSVLTKDAFNSNIKLTQLTISDNKIKVIEAGTFST------LSGLQMIYLHGNPYHCDCKLNPLVQWL 324 HSP 2 Score: 77.7962 bits (190), Expect = 1.862e-15 Identity = 95/337 (28.19%), Postives = 155/337 (45.99%), Query Frame = 2
Query: 131 TFCLLLVIYNLVCLYVFPCPIPCKCTHHS------VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNN---EFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDS--KLHHLDLSENSMLKSIDNDAFRGLDNLQILNL------AKCKISDPDIRFSAMAK 1090
+ C ++++ V V CP CKC S VDC Q +K VP I D + + L+ N I ++ N+FQ L +LK + L N + + AF G +L+ ++L N++ L + F +G+ L ++ LG N F + ++ L++ N I S + +F L L L N+I++I+ FV LKN L N + IA SL NLE+ L N+ I + F G T + L + ++ ++ D F+S KL L +S+N +K I+ F L LQ++ L CK+ +P +++ A K
Sbjct: 8 SVCWVVLVLVGVSRQVLGCPSGCKCAKQSQQDGLDVDCGDQRMKSVPSKIPSDASTIKLANNEITNIGANAFQNLNHLKNIHLESNHISGFDSRAFAGAKSLQMINLYGNNIRSLQSKAF-QGVPSLKYLILGKNSILSFTSSTFQGLSELSDLDMPMNNITS-LPALAFQHTPSLVILDLAYNKIQQISPKAFVGLKNLDYLNLDN--NKITTIAPGTL----QSLQNLEML--VLNNNNIKRVTSSTFKGLTNLRELYFSNNMVSVLTKD----AFNSNIKLTQLTISDNK-IKVIEAGTFSTLSGLQMIYLHGNPYHCDCKL-NPLVQWLATTK 328
BLAST of Epiphycan vs. Ensembl Sea Lamprey
Match: LINGO2 (leucine rich repeat and Ig domain containing 2 [Source:HGNC Symbol;Acc:HGNC:21207]) HSP 1 Score: 82.8037 bits (203), Expect = 2.118e-16 Identity = 95/409 (23.23%), Postives = 166/409 (40.59%), Query Frame = 2
Query: 134 FCLLLVIYNLVCLYVFPCPIPCKCTH--HSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIF 1354
L+ V+ ++ CP C C+ SV C + L +P+GI + +LDLS N +++LS F + L+ L+L+ N + + AF GL +LR L + N + ++ P F LGN +T+L++ NKI ++D +F L L + NE+ + + F +AA L L LE L + + +AL + G L L+ G+ ++ + +L L++ +L+++ +F L NL L++ I+ + A+ L H L+ L L N I + F +LT L+EL +++ +L +E + + L L+L N L +E G
Sbjct: 28 LALITVVLPVLESSAMGCPARCDCSAPLRSVVCHRRKLVSIPEGIPTETRLLDLSKNRLRALSAGDFASHGQLEELQLNENVIAGVEPGAFGGLPHLRALGMRGNRIKLIPPGVF---------AGLGN-------------LTRLDVSENKI-VVLLDFAFRDLRRLLELRVGDNELVYVTRHAFA------------------GLAA---------LRELALERCNL--TAVPTEALAQLH---GLSSLRLRHLGIAAVKPHAFQRL--PRLRSLEIDHWPLLETLGAGSFSSL-NLTSLSVTNTNIT--RVPRLALQTLVH------------------LRTLDLSFNAIASVEAGAFATLTRLRELRMERTGLLVVEPRALQGVEQLGLLNLSGNGLRTLEEGAL 358 HSP 2 Score: 59.3066 bits (142), Expect = 4.133e-9 Identity = 35/124 (28.23%), Postives = 57/124 (45.97%), Query Frame = 2
Query: 1040 AKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLL 1411
A+C S P L + + L++ P +P + L L N +R + DF S L+EL L +N I +E +F L L +L + NR+ I G+F + L L++ + V+L
Sbjct: 47 ARCDCSAP---------LRSVVCHRRKLVSIPEGIPTETRLLDLSKNRLRALSAGDFASHGQLEELQLNENVIAGVEPGAFGGLPHLRALGMRGNRIKLIPPGVFAGLGNLTRLDVSENKIVVL 161
BLAST of Epiphycan vs. Ensembl Sea Lamprey
Match: tlr21 (toll-like receptor 21 [Source:ZFIN;Acc:ZDB-GENE-040220-4]) HSP 1 Score: 82.4185 bits (202), Expect = 3.740e-16 Identity = 141/527 (26.76%), Postives = 240/527 (45.54%), Query Frame = 2
Query: 203 CTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI------------------------PVLKATH----MTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEIN-QTDFVXXXXXXXXXXXXXMN--SLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALT----------------GFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDN-LQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFN--RLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKL-NENLFSGSVNIQLIDLSMNSLI 1630
C H ++ +A+ D+ +G + IL+LS N + + NNSF L +L L++ N + I AF GLS L++L+L N++S L P FN GL+ L + + NN E V++A + + LNL+SN++ +V K L ++ + L+ N I+ ++ + DF+N + + N L + + + ++ S+ NL + + LN+ I + LT N L L + ++++S F ++ LDLS N +KS++ F +N L+ L L K +I ++ S + K DN++ LP L+ + ++ NNI Q+ F + NL++L L N IL + F L +L+ L L N R ++S F + L SL L +L + S L+NLE ++L GN + + G F L L NL L N I L N +F+G +++++DL N ++
Sbjct: 40 CVHRNIHELEEAISDL-RGAPV---ILNLSNNALTVIRNNSFIRLGSLIHLRMDINHIYVIEAGAFNGLSQLQHLNLSFNNISRLIPGAFN-GLVDLKQLIIDNNRLEPTLEMFKPLKNVIQLSLTNNGLSNFSQVVEAIYELPLLQSLNLESNRLTKIIVGKDLRLQETVELMFLSNNSIESLSLERDFLNHITSLDLFNSSLTNVSELAGLNMSSLLQLRLSMRNLNVSKLQTLNN-IFPKTLTFAYFPLGSLPNLHLVCRLNVTRQVMSLTLTNSYISNVSESFCNC---REIQSLDLSFNR-IKSVEKYNFLSPNNTLRNLTLNKNQI---EVLESCVKK-------DNSISVICTPLPY-LENVFIKYNNIFQLESYAFVHMKNLRKLDLTGNDILRLSKRPFHGLHNLSHLVLANNVIRSLNVQSP-FSPLENLKSLMLRNNRLKILNRSVFS-NLTNLEILDLGGNALSY--LHGETFSELSNLKNLYLDRNAIRSLDNRAVFAGLHSLRVLDLVKNMIV 541 HSP 2 Score: 67.0106 bits (162), Expect = 2.055e-11 Identity = 84/304 (27.63%), Postives = 137/304 (45.07%), Query Frame = 2
Query: 947 LHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIR--FSAMAKLTHLFMNDNNLINCPHRLPASL---KFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNS-FDDLASLTSLDLGFNRLTR----IESGIFHRMPKLISLNLEQQNPVLLTIAPNSFV--LSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRD--NQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNY----LPIDFAKLKNISLENNQWNCSC 1804
L LDL+ N +L+ + F GL NL L LA I +++ FS + L L + +N L + ++L + L L N + + + F L+NLK L+L +N+I S++ + F L SL LDL N + I + F + LI+L L Q P + P + L LE ++L GN++ F G FD + L L + D + + +N+ +F + +DL + L + F N+ L T+ ++ N+ Y L LK + L NN +C+C
Sbjct: 408 LRKLDLTGNDILR-LSKRPFHGLHNLSHLVLANNVIRSLNVQSPFSPLENLKSLMLRNNRLKILNRSVFSNLTNLEILDLGGNALSYLHGETFSELSNLKNLYLDRNAIRSLDNRAVFAGLHSLRVLDLVKNMIVYNSPTIRNPPFVELNNLINLKLSAQQPYGMKSIPKNLFKGLYALEKLQLGGNKLLFHG--GHPFDGMHNLKYLDIHDVCSGLQIMNKTMFYKLKKLSSLDLGNDGLGSINNQQFQ-----------NLTHLETLYLNDNAIRYVTEELITPMTNLKYLDLRNNPIDCTC 697
BLAST of Epiphycan vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001020.1 (pep scaffold:Pmarinus_7.0:GL477382:11904:26610:1 gene:ENSPMAG00000000923.1 transcript:ENSPMAT00000001020.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 73.9442 bits (180), Expect = 8.280e-16 Identity = 44/130 (33.85%), Postives = 71/130 (54.62%), Query Frame = 2
Query: 212 HSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVL---KATHMTKLNLDSNKIRS 592
+ VDC+ + L+ VP GI +D +L L FN KSL +F+ L ++ L L ++ ++E F NL++L L N L+ L F++ L +L +YLG N+ +P + T + KL L +N+I S
Sbjct: 1 NEVDCQSKGLQAVPPGIPVDTAMLRLDFNKFKSLDATAFEDLGSVTYLGLESAGIESLSEGVFDHFPNLQSLWLNRNVLATLPLGVFDR-LTQLDKLYLGTNQITSLPTTVFDRLTQLDKLYLGTNQITS 129
BLAST of Epiphycan vs. Ensembl Nematostella
Match: EDO48179 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK32]) HSP 1 Score: 85.1149 bits (209), Expect = 1.239e-16 Identity = 143/541 (26.43%), Postives = 224/541 (41.40%), Query Frame = 2
Query: 131 TFCLLLVIYNLVCLYVFPCPI---------PCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRL----KLSHNEMKEINENAFVG--------------------LSNLRNLHLENNDLSVLHP-ETFNKGLIKLLHVYLGNNEFEKIPVLKATH---MTKLNLDSNKIRSAVVDKSFLLNTGLQTLI----LTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLI 1630
TF L++ ++C++V PC C + V+C + L +P GI D+T L+L +N I L LK L S N + + N G ++++ + HL +N++ + P ++F L+ L L +N+ IP L T + L L+SNKI S++ +F + LQ L L + E + D + N L ++ ++A ++ SL L+L N ++I+ A FN + + L L +++I G F + +KL L L N+ +I +F GL +LQ L L +IS +T F + + L+ L+L+ N + I+ FK L LK L+L N I I SF DL SL LDL N IS +E+ N L+NL S+ L GNRI A A F L + L+L N IT + EN F G +Q + L+ + L+
Sbjct: 7 TFSWLVLYIWILCVFVSSQQTEGTACLEIKPCYCLGNRVNCEGKHLTAIPVGIPSDITALNLGYNNIVDLDPKQLLKFTALKELFNPIPGSFNPIPG-SFNPIPGGFNPIPGGFNPILVASIPFLVASILSCHLHHNNIRGIPPWDSFPSTLMTLT---LHHNKIVAIPSLNTTKQHALRNLYLNSNKI-SSIGHHAFTNLSNLQNLYSIAYLRYTSLTEYKKEDCIKKLGK---------NRLTSVPSDALSQLQ-SLKRLDLSRN--FFTSILASA---FNRLSSLEVLKLSKNRISTIRGAF---WGQNKLQQLYLDRNN-FTAIQTSSFFGLRDLQNLYLQSNQIS---------TIITTKFADWRSF--------PVLRKLNLERNRLSHIQDTTFKHLLALKVLNLANNRIYHISQGSFSDLRSLEGLDLSNND---------------ISWTVEEMNGPFRG-------LTNLASLRLDGNRIT--AIAATAFLGLENIKYLNLSANIITSIQENSFQGMDKLQKLWLNTSQLM 482
BLAST of Epiphycan vs. Ensembl Nematostella
Match: EDO43258 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZD5]) HSP 1 Score: 61.2326 bits (147), Expect = 2.020e-9 Identity = 97/358 (27.09%), Postives = 152/358 (42.46%), Query Frame = 2
Query: 320 NSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRS----AVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKL 1372
+ + L L+ L L+ N + ++ NAF L L L L+ NDL+V+ P+ K L +L H+ L N K+P K + +LNL NK++S A+ D L+ LQ L L N I+ I D +LT + L LE + AF + L+ GN L + G Q L L+S + + +F F KL+ + + N + + D G ++++L L +IS MA+L K L L+ N I ++ + FK + +L+ L L KN I I N+F L +LDL N R+ S ++ +L
Sbjct: 53 DGLKALSRLQHLNLNFNRISKVPRNAFNKLRLLVELFLDYNDLTVV-PDGL-KALSRLQHLNLNFNRISKVPRNAFNKLRLLVELNLRGNKLKSIPTSAIND---LMR--LQQLELDGNPIRVIK--DHAFKGLFRLTEIALGWEKLEEVYHNAFT--ELPLLRRLPLGNVKL------KHFPNLTGTHSLQILTLESNYIEYLPRNFCGMF--PKLYDFNANMNKIKRIPD---MSGCKSMELLKLVHNQISSIGSSLQGMARL---------------------KDLTLEGNRITEVNDNTFKGVKSLETLDLAKNQIKHISKNAFSHFERLQTLDLSENLFVRLPSAGMQQIRRL 367 HSP 2 Score: 54.299 bits (129), Expect = 2.904e-7 Identity = 70/277 (25.27%), Postives = 115/277 (41.52%), Query Frame = 2
Query: 998 DAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPA--SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDL--------------GFNRLTRIESG------IFH-RMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKF-------------------------------------------DATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSL 1627
D + L LQ LNL +IS P F+ + L LF++ N+L P L A L+ L+L N I ++ ++ F L L EL+L+ N + SI T++ +DL L L+L G RLT I G ++H +L L V L PN +L+ + L+ N I++ ++ G + +L +L+L N+IT++N+N F G +++ +DL+ N +
Sbjct: 53 DGLKALSRLQHLNLNFNRISKVPRNAFNKLRLLVELFLDYNDLTVVPDGLKALSRLQHLNLNFNRISKVPRNAFNKLRLLVELNLRGNKLKSIPTSAINDLMRLQQLELDGNPIRVIKDHAFKGLFRLTEIALGWEKLEEVYHNAFTELPLLRRLPLGNVKLKHFPNLTGTHSLQILTLESNYIEYLPRNFCGMFPKLYDFNANMNKIKRIPDMSGCKSMELLKLVHNQISSIGSSLQGMARLKDLTLEGNRITEVNDNTFKGVKSLETLDLAKNQI 329 HSP 3 Score: 53.5286 bits (127), Expect = 5.217e-7 Identity = 103/378 (27.25%), Postives = 157/378 (41.53%), Query Frame = 2
Query: 365 HNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLP-----ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNR 1474
H + + I N F L+ NDL+V+ P+ K L +L H+ L N K+P K + +L LD N + VV + LQ L L N I ++ + F K L L L+ N L++I A + L LEL+GN + I + A G F+ ++ + G + + F + L N LK N G +LQIL L I F M + F + N I R+P S++ L L N I I + + LK+L L+ N I + N+F + SL +LDL N++ I F +L +L+L + V L A + + + L+GNR
Sbjct: 21 HRDQQSIFRNTFFPYCCTLFRFLDYNDLTVV-PDGL-KALSRLQHLNLNFNRISKVPRNAFNKLRLLVELFLDYNDL--TVVPDGLKALSRLQHLNLNFNRISKVPRNAF--NKLRLLVELNLRGNKLKSIPTSAINDLMR-LQQLELDGNPI--RVIKDHAFKGL-----FRLTEI-ALGWEKLEEVYHNAFTELPLLRRLPLGNVKLKHFPN--LTGTHSLQILTLESNYIEYLPRNFCGMFPKLYDFNANMNKIK---RIPDMSGCKSMELLKLVHNQISSI-GSSLQGMARLKDLTLEGNRITEVNDNTFKGVKSLETLDLAKNQIKHISKNAFSHFERLQTLDLSENLFVRLPSAG----MQQIRRLYLRGNR 373
BLAST of Epiphycan vs. Ensembl Nematostella
Match: EDO35407 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLJ8]) HSP 1 Score: 58.5362 bits (140), Expect = 4.268e-9 Identity = 69/258 (26.74%), Postives = 116/258 (44.96%), Query Frame = 2
Query: 1154 LKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIK--FDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNIS---LENNQWNCSCHQ---IQWLLHFRKLILNRDQILCNIPNEKAGKNL 1900
LK+L L +N+I I+ F++L LK+L+L +N I ++ + F L SL LDL FN L + G+F + L L L + I +F L NL + + +K T G + +L L L + +N +T + + ++ +DLS N LI + + T S + L+ N + I D F++LK + L N + C C WL + + L+ + C P++ G++L
Sbjct: 20 LKWLDLSNNDISDIQDGTFENLRYLKQLNLGENKIRTVSASMFKGLVSLEKLDLRFNDLKALPRGVFKPLKSLRELFLHDNRNMGSRIEEGAFNGLDNLRKLHVSATGMKKITQGTFGSLM-SLEFLDVLHINNNSLTTVPLHALRKVKFLKELDLSAN-LISYVSSTAFANNSKLQKLLLHNNTIRLIHED---------AFSQLKGLRALFLRGNPFQCDCQLNGFHAWLRNNQYLVNFNGHMYCMTPDKLTGRDL 266 HSP 2 Score: 54.6842 bits (130), Expect = 7.754e-8 Identity = 50/151 (33.11%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 254 KGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKL--SHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGL-IKLLHV-YLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDF 685
KG+ + + LDL FN +K+L F+ L +L+ L L + N I E AF GL NLR LH+ + + TF + ++ L V ++ NN +P + K + +L+L +N I S V +F N+ LQ L+L N I+ I++ F
Sbjct: 63 KGL-VSLEKLDLRFNDLKALPRGVFKPLKSLRELFLHDNRNMGSRIEEGAFNGLDNLRKLHVSATGMKKITQGTFGSLMSLEFLDVLHINNNSLTTVPLHALRKVKFLKELDLSANLI-SYVSSTAFANNSKLQKLLLHNNTIRLIHEDAF 211
BLAST of Epiphycan vs. Ensembl Nematostella
Match: EDO44877 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUM0]) HSP 1 Score: 55.4546 bits (132), Expect = 5.167e-9 Identity = 33/104 (31.73%), Postives = 53/104 (50.96%), Query Frame = 2
Query: 185 CPIPCKCTHHS----VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGL 484
CP C+C S ++C +P I ++ T L S I +L N+FQ L NL+ +++S+N+++ I + F GL+ L L L N + + F KGL
Sbjct: 30 CPSVCQCGERSLVKYIECSRANFTVLPNDIPVETTELYFSRTQISNLVENAFQQLPNLEIIEISNNQIENITDGVFRGLTKLTELRLPENGIKRISVNAF-KGL 132
BLAST of Epiphycan vs. Ensembl Nematostella
Match: EDO47109 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNB0]) HSP 1 Score: 59.3066 bits (142), Expect = 6.687e-9 Identity = 78/278 (28.06%), Postives = 131/278 (47.12%), Query Frame = 2
Query: 221 DCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKI---PVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGF-QFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLA 1042
DC + L+ +P + ++ L L+ N I++L +F ++L+ L LS+NE+ I F L L+ L L +N L V++ ETF +GL L ++ L +N I + + L++ N++ + L++ L L L N ++ + + +T L L N LE +AF + SL L L+GNKL ++ F F Q LD+ L I+ D L + LDL+EN++ K I A RG+ L+ ++L+
Sbjct: 57 DCSNATLQTIPTSLPRNLGSLFLNTNHIRALQAKAFANYLSLRVLDLSNNELSVIANGTFKSLGKLKTLTLNSNRLEVVYAETF-RGLRDLRYLSLRHNSIRTIGDGAFRFSAFLICLDISFNRLSEFKTETLRGLSSTLTELRLRSNILRRV-----AIKRRFNITLLDLGDNLLEVFEVDAFDGL--SLRKLRLDGNKL------DEIPEAFAKVGHFLQELDMSGNYLHEITSDQLLDLH--AIERLDLNENNISK-IRIGALRGMKKLRQISLS 317 HSP 2 Score: 55.4546 bits (132), Expect = 9.633e-8 Identity = 66/229 (28.82%), Postives = 106/229 (46.29%), Query Frame = 2
Query: 1148 ASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIK-FDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAID-IRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLH 1828
LK L L SN + + + F+ L +L+ L L+ NSI +I +F A L LD+ FNRL+ ++ + ++ L ++ +L +A N+ ++L N ++ F+ A FD L L L L N++ ++ E +Q +D+S N L + L L AI+ + LN N +S I I KL+ ISL N +C C + WL H
Sbjct: 120 GKLKTLTLNSNRLEVVYAETFRGLRDLRYLSLRHNSIRTIGDGAFRFSAFLICLDISFNRLSEFKTETLRGLSSTLT-ELRLRSNILRRVAIKRRF--NITLLDLGDNLLEVFEVDA---FDGL-SLRKLRLDGNKLDEIPEAFAKVGHFLQELDMSGNYLHEITSDQLLDL--HAIERLDLNENNISKIRIG------ALRGMKKLRQISLSGNPLSCDCRLV-WLKH 332
BLAST of Epiphycan vs. Ensembl Medaka
Match: lgr6 (leucine rich repeat containing G protein-coupled receptor 6 [Source:NCBI gene;Acc:101156653]) HSP 1 Score: 102.064 bits (253), Expect = 1.797e-21 Identity = 121/441 (27.44%), Postives = 183/441 (41.50%), Query Frame = 2
Query: 185 CPIPCKCTHH----SVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-----------------------PETFNKGLIKLLHVYLGNNEFEKIPVL----------------KATH-----------MTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRLPAS-LKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRI 1339
CP C C +VDC L VP +S T LDLS N I + +F L L L++S N+++ I+ A GL NLR L L+NN L L P +G+ L H++L +N +IPV+ + TH + L+L +N I+S + + F L+TL L N+++E +KL L N+++ I AFV GN L + + F G + FQFL KLH L L+ + ++ + +G +L+IL L + +S P + +L L ++ N + + P S L+ + LQ N IR+I F+ LT+LK L L N + SI ++F L SL LDL NRL+ +
Sbjct: 37 CPFHCHCEEDGIFLTVDCSEIGLSSVPSNLSPFTTYLDLSMNNISEIQPRAFHHLHLLSELRISGNQLRYISGQALQGLHNLRVLMLQNNQLERLPDDAPWDLPNLLSLRLDANLLSDVPSEAFEGVRSLRHLWLDDNSLTEIPVMALDSLPSLQAMTLALNQITHIPDYAFANLSALVVLHLHNNHIQS-MGSRCFEGLQSLETLDLNYNDLQEF---PVAVRTLSKLQELGFHNNNIKAIPERAFV------------GNPQLQTIHFYENPIQFVGKSAFQFL--------------------PKLHTLSLNGATQIQEFPD--LKGTTSLEILTLTRAGLSALPLDLCEQLPRLRVLELSHNQIEDLPSFYHCSALQEIGLQHNKIRRIESSTFQQLTSLKALDLSWNVLESIHPDAFVSLHSLIKLDLSENRLSSV 439
BLAST of Epiphycan vs. Ensembl Medaka
Match: lgr4 (leucine rich repeat containing G protein-coupled receptor 4 [Source:NCBI gene;Acc:101167435]) HSP 1 Score: 95.9005 bits (237), Expect = 1.555e-19 Identity = 119/428 (27.80%), Postives = 174/428 (40.65%), Query Frame = 2
Query: 185 CPIPCKCTH-HSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLH-----------------------PETFNKGLIKLLHVYLGNNEFEKIPVLKATH---MTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVK---------NSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQS-----NNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIES 1345
C C C DC + L VP G+S LDLS N I L N F++ ++ L+L+ N++ I+ A GL L+ L L+NN L + P+ +GL +L H++L +N ++PV TH + L L N+I + + +F T L L L N I+EI F L N + L + EA +K +S+ N LL + + F G T FQ L S+LH L L + ++ D G NL+ L L+ KIS + +L N I +LP+ +H+Q N I+QI +D F+SL+ L L L +N I I ++F L SL +LDL N L I S
Sbjct: 28 CSPSCSCDEDGGADCSGRGLTAVPTGLSTFTYYLDLSMNNITELPANVFKSFPYMEELRLAGNDLTFIHPEALSGLHQLKVLMLQNNQLKSVPSAALKNLQSLQSLRLDANHITTVPDESFQGLQQLRHLWLDDNHLTQVPVGSLTHQANLQALTLALNRI-TYIPANAFANLTSLVVLHLHNNRIREIASNSFAGLVNLETLDLNFNNLMVFPKPIEALPKLKELGFHSNGISSIPEGAFHNNPLLRTIHLYDNPLSFVGTTAFQNL--------------------SELHSLILRGANKMR--DFPVLTGTKNLESLTLSGTKISSLPMDLCENLRLLRTLDLSYNEIE---QLPSFQGCIHIQEISFQHNKIQQIDRDTFQSLSALHLLDLSRNQIQVIHRDAFLTLTSLNNLDLSMNSLATIPS 429 HSP 2 Score: 70.4774 bits (171), Expect = 8.261e-12 Identity = 92/339 (27.14%), Postives = 137/339 (40.41%), Query Frame = 2
Query: 920 FLKPFFDSKLHHLD--LSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCP-----HRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGF-----------------------NRLTRIESGIFHRMPKLISL----------------NLEQQNPVLLTIA------PNSFVLSNLESIELQGNRI-------------------------KFDATAGPI-------------------FDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAK 1645
F+ P S LH L + +N+ LKS+ + A + L +LQ L L I+ PD F + +L HL+++DN+L P H+ A+L+ L L N I I + F +LT+L LHL N I I +NSF L +L +LDL F N ++ I G FH P L ++ NL + + ++L A P NLES+ L G +I + + G I F +L L L L NQI ++ + F ++ +DLSMNSL P++
Sbjct: 94 FIHPEALSGLHQLKVLMLQNNQLKSVPSAALKNLQSLQSLRLDANHITTVPDESFQGLQQLRHLWLDDNHLTQVPVGSLTHQ--ANLQALTLALNRITYIPANAFANLTSLVVLHLHNNRIREIASNSFAGLVNLETLDLNFNNLMVFPKPIEALPKLKELGFHSNGISSIPEGAFHNNPLLRTIHLYDNPLSFVGTTAFQNLSELHSLILRGANKMRDFPVLTGTKNLESLTLSGTKISSLPMDLCENLRLLRTLDLSYNEIEQLPSFQGCIHIQEISFQHNKIQQIDRDTFQSLSALHLLDLSRNQIQVIHRDAFLTLTSLNNLDLSMNSLATIPSR 430 HSP 3 Score: 58.5362 bits (140), Expect = 4.132e-8 Identity = 58/204 (28.43%), Postives = 94/204 (46.08%), Query Frame = 2
Query: 1109 NDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSIL------------------------SIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVL--SNLESIELQGNRIKF-DATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWP 1639
+ L P L +L L NNI ++ + FKS ++EL L N + S+ + + +L SL SL L N +T + F + +L L L+ + LT P + +NL+++ L NRI + A A F NL LV L L +N+I ++ N F+G VN++ +DL+ N+L+ +P
Sbjct: 43 SGRGLTAVPTGLSTFTYYLDLSMNNITELPANVFKSFPYMEELRLAGNDLTFIHPEALSGLHQLKVLMLQNNQLKSVPSAALKNLQSLQSLRLDANHITTVPDESFQGLQQLRHLWLDDNH---LTQVPVGSLTHQANLQALTLALNRITYIPANA---FANLTSLVVLHLHNNRIREIASNSFAGLVNLETLDLNFNNLMVFP 240
BLAST of Epiphycan vs. Ensembl Medaka
Match: lrig1 (leucine rich repeats and immunoglobulin like domains 1 [Source:NCBI gene;Acc:101171324]) HSP 1 Score: 93.9745 bits (232), Expect = 6.040e-19 Identity = 112/396 (28.28%), Postives = 179/396 (45.20%), Query Frame = 2
Query: 128 FTFCLLLVIYNLVCL---------YVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPV--LKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS----DPDIRFSAMAKLTHLFMNDNNLINCPHRLPA---SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSIL 1261
F F LV+Y ++ + PC C C SVDC L P + + + L N I+S+ + L++++ L LS+NE+ E+ + F +R+L+L NN +SVL ++ L + L N +IP+ + +T+L L+ N+IR + +F + L+ L L +N I ++ TD K+ L L+ N+L + + + + SL L L N++I G + L+L LT + L D LH L L NS + I+ AFRGL ++IL L IS D + FS + +L L + N + + + SL+ L+L N IR I+ D F + NLK L + NS L
Sbjct: 8 FGFVCRLVLYLMLSMGERNSSGLGSELPCAQNCTCDGDSVDCSRLELTATP--LDLPARTVSLHHNNIRSIDGRRIRELVSVETLDLSNNEITELRGHCFPAGLQIRDLYLSNNKISVLELGALDRLGSSLQVLRLSRNRISQIPIRAFQLPRLTQLELNRNRIRQ-IEGLTFQGLSSLEVLKLQRNSISKL--TDGAFWDLAKMKVLHLEYNNLTEVNSGSLYGL-TSLTQLFLS-----NNSIARINPDGLKFCQRLRELNLSYNNLTRLDEGSLAMLGD--LHTLRLGHNS-ISQINEGAFRGLKAVRILELDHNDISGTIEDTNGAFSGLDRLNKLTLFGNKIKSVAKEAFSGLESLEHLNLGENPIRSIQPDAFSKMRNLKNLIMDSNSFL 389 HSP 2 Score: 67.3958 bits (163), Expect = 7.621e-11 Identity = 82/293 (27.99%), Postives = 132/293 (45.05%), Query Frame = 2
Query: 1019 NLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRI-KFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNM-----NKLSTISID-FNSTNYL--------------PIDFAK---LKNISLENNQWNCSCHQIQWL 1822
+LQ+L L++ +IS IR + +LT L +N N IRQI F+ L++L+ L L++NSI + +F DLA + L L +N LT + SG + + L L L N + I P+ L + L N + + D + L L L L N I+++NE F G +++++L N + G T S +D RLN NK+ +++ + F+ L P F+K LKN+ +++N + C C Q+QWL
Sbjct: 135 SLQVLRLSRNRISQIPIRAFQLPRLTQLELN---------------------RNRIRQIEGLTFQGLSSLEVLKLQRNSISKLTDGAFWDLAKMKVLHLEYNNLTEVNSGSLYGLTSLTQLFLS--NNSIARINPDGLKFCQRLRELNLSYNNLTRLDEGS---LAMLGDLHTLRLGHNSISQINEGAFRGLKAVRILELDHNDISGTIEDTNGAF--SGLD-RLNKLTLFGNKIKSVAKEAFSGLESLEHLNLGENPIRSIQPDAFSKMRNLKNLIMDSNSFLCDC-QLQWL 397
BLAST of Epiphycan vs. Ensembl Medaka
Match: lingo4b (leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 [Source:NCBI gene;Acc:101164675]) HSP 1 Score: 92.8189 bits (229), Expect = 7.884e-19 Identity = 111/409 (27.14%), Postives = 172/409 (42.05%), Query Frame = 2
Query: 185 CPIPCKCTH--HSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPV 1405
CP C C V C + L VP+G S D LDLS N IK++ F L+ L+ L LS N + I AF+GL NL+ L ++NN L +L F+ GL L + L NE +D +F LQTL +N++ I+Q AF ++N L L L+ + L ++I +ALT T + L ++S+ + + +L L + L +I +++ GL NL L ++ C ++ + ++A+ L +L +FL L N I I+ + L L+ELHL S+L IE +F L+ L++ N LT +E FH + L L L+ NP+
Sbjct: 28 CPSRCACRPEVREVICSGKYLNSVPEGFSNDARRLDLSRNKIKTVGRRQFSGLVQLQDLDLSDNLISMIEVEAFLGLKNLKTLRIKNNRLKILPVGVFS-GLYSLRLLDLSQNEI----------------------LVFLDYTFKEVANLQTLEAGENDLVFISQ--------------------------RAFFGLQN-LQELNLDRSNL--TSIPTEALTQLQSLTVIRMFRLT---ISSLPNNAFRRL--PRLRTLQILNWPALDTIASNSLIGL-NLTSLTISSCNLT--AVPYAALRHLVYL------------------RFLDLSYNPITVIQGNLLGDLLRLQELHLAGASLLRIEPGAFKGLSFFRMLNVTSNMLTTLEESAFHSVGNLQVLRLD-GNPL 357 HSP 2 Score: 71.633 bits (174), Expect = 3.174e-12 Identity = 91/321 (28.35%), Postives = 156/321 (48.60%), Query Frame = 2
Query: 944 KLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLI---NCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRD---NQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID-FNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFR-KLILNRDQILCNIPN 1879
+L LDLS+N ++ I+ +AF GL NL+ L + ++ P FS + L L ++ N ++ + + A+L+ L N++ I Q F L NL+EL+L ++++ SI T + L SLT + + ++ + + F R+P+L +L + P L TIA NS + NL S+ + + TA P + L LV L D N IT + NL + +Q + L+ SL+ F L S + + N L+T+ F+S L+ + L+ N C C ++ W++ R +L + Q C+ P+
Sbjct: 82 QLQDLDLSDN-LISMIEVEAFLGLKNLKTLRIKNNRLKILPVGVFSGLYSLRLLDLSQNEILVFLDYTFKEVANLQTLEAGENDLVFISQRAFFGLQNLQELNLDRSNLTSIPTEALTQLQSLTVIRMFRLTISSLPNNAFRRLPRLRTLQILNW-PALDTIASNSLIGLNLTSLTISSCNL----TAVP-YAALRHLVYLRFLDLSYNPITVIQGNLLGDLLRLQELHLAGASLLRIEPGAFKGL-SFFRMLNVTSNMLTTLEESAFHSV-------GNLQVLRLDGNPLACDC-RLLWVMRRRQRLNFDGHQPTCSSPD 386
BLAST of Epiphycan vs. Ensembl Medaka
Match: lingo4b (leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1 [Source:NCBI gene;Acc:101164675]) HSP 1 Score: 92.8189 bits (229), Expect = 7.884e-19 Identity = 111/409 (27.14%), Postives = 172/409 (42.05%), Query Frame = 2
Query: 185 CPIPCKCTH--HSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPV 1405
CP C C V C + L VP+G S D LDLS N IK++ F L+ L+ L LS N + I AF+GL NL+ L ++NN L +L F+ GL L + L NE +D +F LQTL +N++ I+Q AF ++N L L L+ + L ++I +ALT T + L ++S+ + + +L L + L +I +++ GL NL L ++ C ++ + ++A+ L +L +FL L N I I+ + L L+ELHL S+L IE +F L+ L++ N LT +E FH + L L L+ NP+
Sbjct: 28 CPSRCACRPEVREVICSGKYLNSVPEGFSNDARRLDLSRNKIKTVGRRQFSGLVQLQDLDLSDNLISMIEVEAFLGLKNLKTLRIKNNRLKILPVGVFS-GLYSLRLLDLSQNEI----------------------LVFLDYTFKEVANLQTLEAGENDLVFISQ--------------------------RAFFGLQN-LQELNLDRSNL--TSIPTEALTQLQSLTVIRMFRLT---ISSLPNNAFRRL--PRLRTLQILNWPALDTIASNSLIGL-NLTSLTISSCNLT--AVPYAALRHLVYL------------------RFLDLSYNPITVIQGNLLGDLLRLQELHLAGASLLRIEPGAFKGLSFFRMLNVTSNMLTTLEESAFHSVGNLQVLRLD-GNPL 357 HSP 2 Score: 71.633 bits (174), Expect = 3.174e-12 Identity = 91/321 (28.35%), Postives = 156/321 (48.60%), Query Frame = 2
Query: 944 KLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLI---NCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRD---NQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISID-FNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFR-KLILNRDQILCNIPN 1879
+L LDLS+N ++ I+ +AF GL NL+ L + ++ P FS + L L ++ N ++ + + A+L+ L N++ I Q F L NL+EL+L ++++ SI T + L SLT + + ++ + + F R+P+L +L + P L TIA NS + NL S+ + + TA P + L LV L D N IT + NL + +Q + L+ SL+ F L S + + N L+T+ F+S L+ + L+ N C C ++ W++ R +L + Q C+ P+
Sbjct: 82 QLQDLDLSDN-LISMIEVEAFLGLKNLKTLRIKNNRLKILPVGVFSGLYSLRLLDLSQNEILVFLDYTFKEVANLQTLEAGENDLVFISQRAFFGLQNLQELNLDRSNLTSIPTEALTQLQSLTVIRMFRLTISSLPNNAFRRLPRLRTLQILNW-PALDTIASNSLIGLNLTSLTISSCNL----TAVP-YAALRHLVYLRFLDLSYNPITVIQGNLLGDLLRLQELHLAGASLLRIEPGAFKGL-SFFRMLNVTSNMLTTLEESAFHSV-------GNLQVLRLDGNPLACDC-RLLWVMRRRQRLNFDGHQPTCSSPD 386
BLAST of Epiphycan vs. Planmine SMEST
Match: SMESG000057458.1 (SMESG000057458.1) HSP 1 Score: 1513.05 bits (3916), Expect = 0.000e+0 Identity = 824/824 (100.00%), Postives = 824/824 (100.00%), Query Frame = 2
Query: 116 MKTRFTFCLLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQILCNIPNEKAGKNLFLVMVNCSDVILSRILSHVEQXXXXXXXXXXXXXXXXXXXXXXXXCRHRKRLKFCKRMSSPSYDILHNSSFENTLGEDIXXXXXXXXXXXGSLCLKDYENEQHCEINSKEEPIYDLELDFDVLYVTDNVNDRDFVRLTVKFVNELTESKVKKQILHCEVLAIDYRFKKMENXXXXXXXXXXXXXNFFLYESAICEEELINEMKLYCDCRIFDSDINQMFYDLIDMNYSS 2587
MKTRFTFCLLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVNLKNTKLTTLTLKMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQILCNIPNEKAGKNLFLVMVNCSDVILSRILSHVEQKSKVKSIVISFVVVIIIVVILVLICRHRKRLKFCKRMSSPSYDILHNSSFENTLGEDINNNNDTRGNFNGSLCLKDYENEQHCEINSKEEPIYDLELDFDVLYVTDNVNDRDFVRLTVKFVNELTESKVKKQILHCEVLAIDYRFKKMENLKEILKERGKKKKNFFLYESAICEEELINEMKLYCDCRIFDSDINQMFYDLIDMNYSS
Sbjct: 1 MKTRFTFCLLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVNLKNTKLTTLTLKMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSFVLSNLESIELQGNRIKFDATAGPIFDNLPKLVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDFNSTNYLPIDFAKLKNISLENNQWNCSCHQIQWLLHFRKLILNRDQILCNIPNEKAGKNLFLVMVNCSDVILSRILSHVEQKSKVKSIVISFVVVIIIVVILVLICRHRKRLKFCKRMSSPSYDILHNSSFENTLGEDINNNNDTRGNFNGSLCLKDYENEQHCEINSKEEPIYDLELDFDVLYVTDNVNDRDFVRLTVKFVNELTESKVKKQILHCEVLAIDYRFKKMENLKEILKERGKKKKNFFLYESAICEEELINEMKLYCDCRIFDSDINQMFYDLIDMNYSS 824
BLAST of Epiphycan vs. Planmine SMEST
Match: SMESG000044588.1 (SMESG000044588.1) HSP 1 Score: 100.138 bits (248), Expect = 1.752e-21 Identity = 98/308 (31.82%), Postives = 160/308 (51.95%), Query Frame = 2
Query: 218 VDCRHQALKDVPKGIS-IDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKLLNSTIVEQALTGFNGATGFQFLDL----KSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNL 1123
+DC+ L+ +P + +V I L FN ++ L N+SFQ LK L+L +N+MK + EN F+GL NL L+L NN L L P + L KL +YL +NEF KIP + + + L L +NKI S KS+L ++T+ L N I+++++ NLK +++ + L NS N+ E F ++ + +++ N L+ ++ + FN + L + GLT +S L + LDLSEN ++ + D FR ++L L+L + KI +F + KL L M+ N++
Sbjct: 19 LDCQRLQLRLIPSCKNQKNVKIFLLGFNQLEILHNSSFQMYSKLKELRLYNNKMKILQENTFIGLINLEILYLNNNFLKQL-PASLLSELPKLRKIYLHHNEFVKIPEISSRSLVNLYLGNNKIYSLYFPKSYLNCKKMRTIDLKHNLIEKLDKNHLYNLKKSQIESFILLSNSFRNVTTEIFYHIRKRIRKIDVSYNPHLSDYGFQKMIASFNHERHKKRWHLDIFASNIGLTKLSNFTLFTLTNVSTQKLDLSEN-FIEDLLTD-FRETNDLLELSLNRNKIFKIAKYQFVNLRKLRKLDMSYNSI 323
BLAST of Epiphycan vs. Planmine SMEST
Match: SMESG000015729.1 (SMESG000015729.1) HSP 1 Score: 77.0258 bits (188), Expect = 7.860e-14 Identity = 109/375 (29.07%), Postives = 175/375 (46.67%), Query Frame = 2
Query: 236 ALKDVP---KGISIDVTILDLSFNLIK------SLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPVLKATHMTKLNLDSNKIRSAVVDKS-FLLNTGLQTLILTKNEIK----EINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLINLELEGNKL---LNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFF---DSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISD-PDIRFSAMAKLTHLFMNDNNLINCPHRL---PASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDD 1288
LKD P + I+ V I++ S NLI SL + F+ L L+ L LS N++K++ + F + L L+L++N + L P TF L +YL NN+F + +K +T IR +D S L+ T Q ++++ E N + + + + L L L N ++ + F+ ++N L+ L L GN+L + ST +E K+C + PF+ + L HLDLS+N L I + GL N+QIL ++ C I+ D F M ++ L ++ NNL P L ++L+ L+L NNI Q+R + L NL+ L+L N ++ T S D
Sbjct: 93 GLKDPPFIQESIN-SVEIINFSNNLIDEGSQFYSLGMHFFKRLPKLRVLNLSKNKLKDLPKVWFECPNQLEILNLDDNMIGGLDP-TFASCFANLKQLYLRNNKFSYLKKIKDVDVT---FSGIPIRPVELDNSDHLIRTPNQLPFNVRHKVSKSSNEPNSHNNIIPQFSTLEILDLSFNKIQALEQIPFIGLQN-LVKLSLRGNRLKSYVKSTSLE-----------------KNCDFGT------APFYIKSPTSLIHLDLSDNIGLTRIHSCWIFGLQNIQILEISNCSINTIGDSTFHKMKNISRLDLSYNNLNELPKELVSISSTLRDLNLARNNISQLRSGNLIRLINLRALNLSHNRFQTLLTFSSKD 438
BLAST of Epiphycan vs. Planmine SMEST
Match: SMESG000068721.1 (SMESG000068721.1) HSP 1 Score: 72.4034 bits (176), Expect = 2.103e-12 Identity = 146/607 (24.05%), Postives = 242/607 (39.87%), Query Frame = 2
Query: 185 CPIPCKCTHHS-VDCRHQALKDVPKGISIDVTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIP---VLKATHMTKLNLDSNKIRSAVVDKSFLLNTGLQTLILTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIAAEAFVPVKNSLI-----NLELEGN-KLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKIS--DPDIRFSAMAKLTHLFMNDNNLI---NCPHRLPASLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSI-LSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLN------------------LEQQNPV--LLTIAPNSFVLSNLESIELQGNRIKFDATA---GPIFDNLPK-LVNLSLRDNQITKLNENLFSGSVNIQLIDLSMNSLIGWPAKTFLPLISSAIDIRLNMNKLSTISIDF--------------NSTNYLPI----DFAKLKNISLENNQWNCSCHQIQWLLHF 1831
C C C+H V C + L ++P+ + +V LDL N I L LK++ L+ N M +I AF+GL+ L +++L N+L + F GL+ L+ +YL N+ E +P + + + L+L NK+ +++ ++F L+ L L N + + ++ T T L+ + P++N I N E E + + V + ++ + GL S+ F +K L +S N++ K + NL L+L+ +I +PD+ F+ +KL + +N N L N + L+ L L N I I D FK+ NLK L L KN + + + LG +RL +S I + + L+ + N V +I S + + K D + I + +PK L L N + LNE F +N++ + LS N + PL S + L+ NKL I D N + +P D L NI+L N ++C C+ I+WL F
Sbjct: 266 CSKKCNCSHDGIVKCVGKRLSNIPQDLPTNVVELDLKDNNITILQQGYLSKYKFLKKIILTRNGMVDIEPGAFLGLTQLTSIYLNENNLKTIRKNAFG-GLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKL-TSLKKETFKPFLNLRYLYLVHNPLNCDCRMSWIVDYIKDKETNTPVAACLQPRNLQG-TPLRNLQIYNFQCNKEFESKYETHDEECVPKVSCPLKCTCSSDVVNCSNSGLMSLPESI---FPSTKT--LIMSHNNLKKLTPIYSIHEAPNLMKLDLSFNQIETLEPDV-FTRSSKLREINLNSNRLKCINNETFKNLKELEILQLSENAISCIVTDSFKNNLNLKYLMLNKNQFHCDCKLKWMSEWSRNHYTVLGNHRLPTCQSPIILKDTPITHLDDKYFLCDGNASVDHSVDSCHETNSVKTCCSIGNEECTKSKKCPSKCKCESTKVDCSDLQLKEIPNEIPKDTTELYLDRNHLQSLNETSFKNLLNLKTLVLSYNGITELNKNVLTPL-KSLETLVLSFNKLQCIHQDAFKNLHNLKVLILQSNDVSTIPYQAFNDLKNLNNIALGQNPFHCDCN-IKWLNQF 861 HSP 2 Score: 68.5514 bits (166), Expect = 2.823e-11 Identity = 122/486 (25.10%), Postives = 204/486 (41.98%), Query Frame = 2
Query: 185 CPIPCKCTHHSVDCRHQALKDVPK------------GISIDV------------TILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNK-GLIKLLHVYLGNNEF---EKIPVLKATHMTKLNLDSNKIRSA---------VVDKSFLLNTGLQTLI------LTKNEIKEINQTDFVXXXXXXXXXXXXXMNSLENIA-AEAFVPVKNSLIN----LELEGNKLLNSTIVEQALTGFNGATGFQFLDLKSCGLTSISGDFLKPFFDSKLHHLDLSENSMLKSIDNDAFRGLDNLQILNLAKCKISDPDIRFSAMAKLTHLFMNDNNLINCPHRLPA---SLKFLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISL--NLEQQNPVLLTIAPNSF---VLSNLESIELQGNR 1474
CP C+C ++SV+C+H+ +VP G I+V +L LS N I S+ +F L+NL +L+L+ N ++ + ++ F L+ L+ L L +N L ++ FNK G++K L + GN F E I LK H+ +L + NK+ + KS ++ G +++ L+ I ++ +F K +LEN+ ++ V K + ++ G +L N + Q L T LDLK +T + +L + L + L+ N M+ I+ AF GL +LT +++N+NNL SL FL+LQ N+I + +D +S L LHL +N + S++ +F +L L L N L RM ++ + E PV + P + L NL+ Q N+
Sbjct: 39 CPNGCQCENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQLQCINDNIFNKLGMLKYL-LLEGNKIFWISEAISKLK--HLKELRIHKNKLNCNCHLAWLNWFLKQKSNIIKPGAHSVLCSTPFRLSHIPISSLSSYEFQCASEDKA--------NLENMCPSDKKVCSKKCNCSHDGIVKCVGKRLSN---IPQDL-----PTNVVELDLKDNNITILQQGYLSKY--KFLKKIILTRNGMV-DIEPGAFLGL-----------------------TQLTSIYLNENNLKTIRKNAFGGLVSLMFLYLQYNDIECLPKDTLESSKRLHLLHLSENKLTSLKKETFKPFLNLRYLYLVHNPLN-----CDCRMSWIVDYIKDKETNTPVAACLQPRNLQGTPLRNLQIYNFQCNK 474 HSP 3 Score: 55.4546 bits (132), Expect = 2.746e-7 Identity = 49/137 (35.77%), Postives = 69/137 (50.36%), Query Frame = 2
Query: 1112 DNNLINCPHRL----PASLK----FLHLQSNNIRQIRQDDFKSLTNLKELHLKKNSILSIETNSFDDLASLTSLDLGFNRLTRIESGIFHRMPKLISLNLEQQNPVLLTIAPNSF-VLSNLESIELQGNRIKFDATA 1495
+N + C HRL P L+ L+L N I IR+ DF L NLK L L N I SIE +FD+L +L L L N + + +F ++ KL L+L L I N F L L+ + L+GN+I + + A
Sbjct: 46 ENYSVECKHRLFPNVPVILQEKTTRLNLLGNRINVIRKSDFLKLNNLKVLQLSNNRITSIEPGAFDNLVNLIKLRLNRNLIQYLPDDLFSKLTKLQKLDLRDNQ--LQCINDNIFNKLGMLKYLLLEGNKIFWISEA 180
BLAST of Epiphycan vs. Planmine SMEST
Match: SMESG000012091.1 (SMESG000012091.1) HSP 1 Score: 58.151 bits (139), Expect = 3.315e-8 Identity = 50/170 (29.41%), Postives = 83/170 (48.82%), Query Frame = 2
Query: 197 CKCTHHSVDCRHQ-ALKDVPKGISID--------------------------VTILDLSFNLIKSLSNNSFQTLINLKRLKLSHNEMKEINENAFVGLSNLRNLHLENNDLSVLHPETFNKGLIKLLHVYLGNNEFEKIPV--LKATHMTKLNLDSNKIRSAVVDKSFLL 619
C+CT +VDC +Q A+ +P I D +T L + ++ ++S+ NN+F ++ L + LSHN I AFVGL+ L+NL+LE N + + GL + +YL NN +I V ++ ++KL L +N I++ +D+ F L
Sbjct: 46 CQCTSKTVDCNYQDAISFIPSDIKEDYDQLHVSHAEFQDPRLMKANFSTIIHLTSLKIVYSKLRSIDNNTFTSMDRLVYMDLSHNNFSVIGSRAFVGLT-LKNLYLEQNLGGLQLGQDMFYGL-TVTDLYLKNNGISQISVGIIEKLQISKLYLFNNSIKT--LDRRFKL 211 The following BLAST results are available for this feature:
BLAST of Epiphycan vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Epiphycan vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Epiphycan vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Epiphycan vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Epiphycan vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Epiphycan vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Epiphycan vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Epiphycan vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Epiphycan vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Epiphycan vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Epiphycan vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Epiphycan vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Epiphycan vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Epiphycan vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003556 ID=SMED30003556|Name=Epiphycan|organism=Schmidtea mediterranea sexual|type=transcript|length=3437bpback to top protein sequence of SMED30003556-orf-1 >SMED30003556-orf-1 ID=SMED30003556-orf-1|Name=SMED30003556-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=825bp MKTRFTFCLLLVIYNLVCLYVFPCPIPCKCTHHSVDCRHQALKDVPKGISback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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