FAS-associated factor 2

Overview
NameFAS-associated factor 2
Smed IDSMED30003540
Length (bp)1234
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of FAS-associated factor 2 (SMED30003540) t-SNE clustered cells

Violin plots show distribution of expression levels for FAS-associated factor 2 (SMED30003540) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of FAS-associated factor 2 (SMED30003540) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for FAS-associated factor 2 (SMED30003540) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003540

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30003540h1SMcG0004857 Contig3755newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003540h1SMcG0004857 Contig3755uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003540h1SMcG0016388 Contig3755newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003540h1SMcG0016388 Contig3755uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30003540h1SMcG0016388 SmedASXL_002250SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30003540h1SMcG0016388 Contig26018newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30003540h1SMcG0016388 dd_Smed_v4_10511_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30003540h1SMcG0016388 dd_Smed_v4_10511_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymal cellSMED30003540h1SMcG0016388 dd_Smed_v4_10511_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30003540h1SMcG0016388 dd_Smed_v6_10511_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30003540h1SMcG0016388 Contig26347newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30003540h1SMcG0016388 Contig26347uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Human
Match: FAF2 (Fas associated factor family member 2 [Source:HGNC Symbol;Acc:HGNC:24666])

HSP 1 Score: 159.458 bits (402), Expect = 1.126e-43
Identity = 116/361 (32.13%), Postives = 182/361 (50.42%), Query Frame = 3 
Query:  135 FPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQ-----KTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            F YY I   F     FI P           D+  F+  FE KYG   P+F    Y   +N AK  L +LL+YLHGDDH+DSD FCR TLC PE ++ I N  +LFWAC+ N  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   M  N   +   R EREER   ++ +Q+   + E +L     ++  E    E    K++     +  KL EE+ +  LQ   K++++  L P       E+ ++I   K P  S  +  F+ + S+ +I+  +F  ++ P +F++  NFP+  + C P++ EW N             PP   EA L+   +L+V D +
Sbjct:  105 FTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLI-NTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQ---QDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQ-EEKERKLECLPPEPSPDDPESVKIIF--KLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCIPSE-EWPN-------------PPTLQEAGLSHTEVLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Celegans
Match: H40L08.1 (pep chromosome:WBcel235:X:15080779:15084086:-1 gene:WBGene00010433.1 transcript:H40L08.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:H40L08.1)

HSP 1 Score: 48.521 bits (114), Expect = 5.906e-6
Identity = 82/304 (26.97%), Postives = 134/304 (44.08%), Query Frame = 3 
Query:  333 YLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEG--------FGVSKIIREHSYPFIGLVAINTNGRMYLVARF---EGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVI----LRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIR---SPRAE-FCDPPDFVEANLTSNAMLYV 1187
            +L+ Y+H +    SD+F  K L    F   I++   +F+ C + S +         FG S+     S   I ++  N+  R  L+      E VST   L   + D +AD++   A  R + E R       +E  +  +R L      ERI     E    KK   A+  EEQ Q     R  ++R++T+   + +IS +T        L I+FP G      F   D+I+ I+     SE CP  F+++Q+FPK  + C P   +W  + +      P++E   +   F EA +T  +M+Y+
Sbjct:  165 WLVTYIH-EPSGSSDFF--KYLFTSNFSELIKSRGGVFFGCVMGSEDAQRLRPDRAFGRSR----RSCILIFVIRGNSLTRKLLIDDLSNPESVSTNIDLA--LIDLIADDADRSARNRVQNESRRLMEEQNREYQESLQRDL------ERIAKSKSEEEAAKK---AQEEEEQKQ-----RDAEERLNTVETYKRTISGDTTLSTGSHDLLIRFPTGKKV-IKFNADDNIEKIFDEAMKSEMCPLFFQMHQSFPKKAVPCLP---KWYFEILSAEELEPKSECLSNNLTFQEAGITHGSMVYI 441
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Fly
Match: Faf2 (gene:FBgn0025608 transcript:FBtr0343115)

HSP 1 Score: 117.087 bits (292), Expect = 1.589e-28
Identity = 80/281 (28.47%), Postives = 145/281 (51.60%), Query Frame = 3 
Query:  222 DVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDY--FCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQK-RVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPK 1055
            DV +FI+ +  +Y    P+F    Y   +N AK  L +L++YLH D  ++ D   FCR TL     +++I N + L W C++ + EG+ V + I   SYP + ++++  N RM +V RFEG  T  +L+  ++     N V ++  RA+R ER   +  +++  +  E+ L      ER +     + + + ++  +      +L  +   +QK  ++TL+P + + +D    + +  K P G+  +  F  TDS+  +Y+ +FC    P EF++  NFPK
Sbjct:  145 DVMKFIREYYERYP-EHPVFYQGTYAQALNDAKQELRFLIVYLHKDPAKNPDVESFCRNTLSARSVIDYI-NTHTLLWGCDVATPEGYRVMQSITVRSYPTMVMISLRAN-RMMIVGRFEGDCTPEELLRRLQSVTNANEVWLSQARADRLERNFTQTLRRQQDEAYEQSLLADEEKER-QRQRERDAVRQAEEAVEQARRDVELRKEEIARQKIELATLVPSEPA-ADAVGAIAVVFKLPSGTRLERRFNQTDSVLDVYHYLFCHPDSPDEFEITTNFPK 420
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Fly
Match: Faf2 (gene:FBgn0025608 transcript:FBtr0081126)

HSP 1 Score: 117.087 bits (292), Expect = 1.589e-28
Identity = 80/281 (28.47%), Postives = 145/281 (51.60%), Query Frame = 3 
Query:  222 DVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDY--FCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQK-RVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPK 1055
            DV +FI+ +  +Y    P+F    Y   +N AK  L +L++YLH D  ++ D   FCR TL     +++I N + L W C++ + EG+ V + I   SYP + ++++  N RM +V RFEG  T  +L+  ++     N V ++  RA+R ER   +  +++  +  E+ L      ER +     + + + ++  +      +L  +   +QK  ++TL+P + + +D    + +  K P G+  +  F  TDS+  +Y+ +FC    P EF++  NFPK
Sbjct:  145 DVMKFIREYYERYP-EHPVFYQGTYAQALNDAKQELRFLIVYLHKDPAKNPDVESFCRNTLSARSVIDYI-NTHTLLWGCDVATPEGYRVMQSITVRSYPTMVMISLRAN-RMMIVGRFEGDCTPEELLRRLQSVTNANEVWLSQARADRLERNFTQTLRRQQDEAYEQSLLADEEKER-QRQRERDAVRQAEEAVEQARRDVELRKEEIARQKIELATLVPSEPA-ADAVGAIAVVFKLPSGTRLERRFNQTDSVLDVYHYLFCHPDSPDEFEITTNFPK 420
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Zebrafish
Match: faf2 (Fas associated factor family member 2 [Source:ZFIN;Acc:ZDB-GENE-050208-312])

HSP 1 Score: 148.673 bits (374), Expect = 5.830e-40
Identity = 102/353 (28.90%), Postives = 174/353 (49.29%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FI  FE KYG + P+F    Y   +N AK  L YLL+YLHG+DH+D+D FCR TLC  E + FI N  +LFWAC+ +  EG+ VS+ +RE++YPF+ ++ +  + +M +V R EG+     L+  +   M  N   +   R EREER   ++ +Q+  +  E  L      +R +    E    +++K  + +  + +       +++R S  LP +  + D+   V +  + P  +  +  F    S+ +I+  +F  ++ P +F+V  N+P+  + C P + E               +PP  ++A L+ + +L+V D +
Sbjct:  108 YTLLDIFRFALRFIRPDPRGRVTDPVGDVMSFIHSFEEKYGRSHPVFYQGTYSQALNDAKRELRYLLVYLHGEDHQDTDEFCRSTLCSEEALTFI-NTRMLFWACSTSKPEGYRVSQALRENTYPFLAMIMLK-DRKMTVVGRLEGLIQPEDLINQLTFIMEANQTYLMSERLEREERNQTQVLRQQQDEAYEASLRADQEKDRKKREEQEQKRQEEEKVRQTVLAEERRRRTLEEEKERRSECLPAEPPV-DDPDGVKIVFRLPNDTRVERRFLFGQSLTVIHDFLFSLKETPEKFQVVTNYPRRVLPCLPTEDE--------------PNPPTLMQAGLSRSEVLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Xenopus
Match: faf2 (uncharacterized LOC733461 [Source:Xenbase;Acc:XB-GENE-5967086])

HSP 1 Score: 159.844 bits (403), Expect = 7.890e-44
Identity = 109/355 (30.70%), Postives = 178/355 (50.14%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHI--THNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FIQ FE KYG   P+F    Y   +N AK  L +LL+YLHG+DH+DSD FCR TLC PE  +F+ N  +LFWAC+ N  EGF VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   +  N   +   R EREER   ++ +Q+  +     L        ++ E    +    ++ +  +++EE+ +  L+   +++R S  LP +  + D    V +  K P G+  +  F  T S+ +I+  +F  ++ P +F++  NFP+  + C P++              E   PP   EA L+ + +L+V D +
Sbjct:  108 YTLLDIFRFAVRFIRPDPRSRVTDPVGDVVSFIQLFEEKYGRIHPVFYQGTYSQALNDAKQELRFLLVYLHGEDHQDSDDFCRNTLCIPEVTHFL-NSRMLFWACSTNKPEGFRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPQDLINQLTFIIEANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKKEKQEQKRREEEEAQLKQMLEERKKRNLE--EEKERKSECLPAE-PVPDHPDNVKIIFKMPNGTRVERRFLFTQSLSVIHDFLFSLKETPEKFQIVTNFPRRVLPCLPSE--------------EIPVPPTLQEAGLSQSQLLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Xenopus
Match: faf2 (uncharacterized LOC733461 [Source:Xenbase;Acc:XB-GENE-5967086])

HSP 1 Score: 159.073 bits (401), Expect = 1.074e-43
Identity = 109/355 (30.70%), Postives = 178/355 (50.14%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHI--THNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FIQ FE KYG   P+F    Y   +N AK  L +LL+YLHG+DH+DSD FCR TLC PE  +F+ N  +LFWAC+ N  EGF VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   +  N   +   R EREER   ++ +Q+  +     L        ++ E    +    ++ +  +++EE+ +  L+   +++R S  LP +  + D    V +  K P G+  +  F  T S+ +I+  +F  ++ P +F++  NFP+  + C P++              E   PP   EA L+ + +L+V D +
Sbjct:  103 YTLLDIFRFAVRFIRPDPRSRVTDPVGDVVSFIQLFEEKYGRIHPVFYQGTYSQALNDAKQELRFLLVYLHGEDHQDSDDFCRNTLCIPEVTHFL-NSRMLFWACSTNKPEGFRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPQDLINQLTFIIEANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKKEKQEQKRREEEEAQLKQMLEERKKRNLE--EEKERKSECLPAE-PVPDHPDNVKIIFKMPNGTRVERRFLFTQSLSVIHDFLFSLKETPEKFQIVTNFPRRVLPCLPSE--------------EIPVPPTLQEAGLSQSQLLFVQDLT 438
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Xenopus
Match: faf2 (uncharacterized LOC733461 [Source:Xenbase;Acc:XB-GENE-5967086])

HSP 1 Score: 159.073 bits (401), Expect = 1.270e-43
Identity = 112/358 (31.28%), Postives = 181/358 (50.56%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQ-----KTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FIQ FE KYG   P+F    Y   +N AK  L +LL+YLHG+DH+DSD FCR TLC PE  +F+ N  +LFWAC+ N  EGF VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   +  N   +   R EREER   ++ +Q+   + E +L     ++  E    E    K++     +  +++EE+ +  L+   +++R S  LP +  + D    V +  K P G+  +  F  T S+ +I+  +F  ++ P +F++  NFP+  + C P++              E   PP   EA L+ + +L+V D +
Sbjct:  112 YTLLDIFRFAVRFIRPDPRSRVTDPVGDVVSFIQLFEEKYGRIHPVFYQGTYSQALNDAKQELRFLLVYLHGEDHQDSDDFCRNTLCIPEVTHFL-NSRMLFWACSTNKPEGFRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPQDLINQLTFIIEANQTYLVSERLEREERNQTQVLRQQ---QDEAYLASLRADQEKERKKKEKQEQKRREEEEAQLKQMLEERKKRNLE--EEKERKSECLPAE-PVPDHPDNVKIIFKMPNGTRVERRFLFTQSLSVIHDFLFSLKETPEKFQIVTNFPRRVLPCLPSE--------------EIPVPPTLQEAGLSQSQLLFVQDLT 447
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Xenopus
Match: faf2 (uncharacterized LOC733461 [Source:Xenbase;Acc:XB-GENE-5967086])

HSP 1 Score: 157.918 bits (398), Expect = 4.375e-43
Identity = 109/355 (30.70%), Postives = 178/355 (50.14%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHI--THNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FIQ FE KYG   P+F    Y   +N AK  L +LL+YLHG+DH+DSD FCR TLC PE  +F+ N  +LFWAC+ N  EGF VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   +  N   +   R EREER   ++ +Q+  +     L        ++ E    +    ++ +  +++EE+ +  L+   +++R S  LP +  + D    V +  K P G+  +  F  T S+ +I+  +F  ++ P +F++  NFP+  + C P++              E   PP   EA L+ + +L+V D +
Sbjct:  123 YTLLDIFRFAVRFIRPDPRSRVTDPVGDVVSFIQLFEEKYGRIHPVFYQGTYSQALNDAKQELRFLLVYLHGEDHQDSDDFCRNTLCIPEVTHFL-NSRMLFWACSTNKPEGFRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPQDLINQLTFIIEANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKKEKQEQKRREEEEAQLKQMLEERKKRNLE--EEKERKSECLPAE-PVPDHPDNVKIIFKMPNGTRVERRFLFTQSLSVIHDFLFSLKETPEKFQIVTNFPRRVLPCLPSE--------------EIPVPPTLQEAGLSQSQLLFVQDLT 458
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Mouse
Match: Faf2 (Fas associated factor family member 2 [Source:MGI Symbol;Acc:MGI:1923827])

HSP 1 Score: 157.147 bits (396), Expect = 3.275e-43
Identity = 115/361 (31.86%), Postives = 181/361 (50.14%), Query Frame = 3 
Query:  135 FPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQ-----KTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            F YY I   F     FI P           D+  F+  FE KYG   P+F    Y   +N AK  L +LL+YLHGDDH+DSD FCR  LC PE ++ I N  +LFWAC+ N  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   M  N   +   R EREER   ++ +Q+   + E +L     ++  E    E    K++     +  KL EE+ +  LQ   K++++  L P       E+ ++I   K P  S  +  F+ + S+ +I+  +F  ++ P +F++  NFP+  + C P++ EW N             PP   EA L+   +L+V D +
Sbjct:   86 FTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNALCAPEVISLI-NSRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQ---QDEAYLASLRADQEKERKKREEKERKRRKEEEVQQQKLAEERRRQNLQ-EEKERKLECLPPEPSPDDPESVKIIF--KLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCVPSE-EWPN-------------PPTLQEAGLSHTEVLFVQDLT 424
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Mouse
Match: Faf2 (Fas associated factor family member 2 [Source:MGI Symbol;Acc:MGI:1923827])

HSP 1 Score: 157.532 bits (397), Expect = 4.226e-43
Identity = 115/361 (31.86%), Postives = 181/361 (50.14%), Query Frame = 3 
Query:  135 FPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQ-----KTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            F YY I   F     FI P           D+  F+  FE KYG   P+F    Y   +N AK  L +LL+YLHGDDH+DSD FCR  LC PE ++ I N  +LFWAC+ N  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   M  N   +   R EREER   ++ +Q+   + E +L     ++  E    E    K++     +  KL EE+ +  LQ   K++++  L P       E+ ++I   K P  S  +  F+ + S+ +I+  +F  ++ P +F++  NFP+  + C P++ EW N             PP   EA L+   +L+V D +
Sbjct:  105 FTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNALCAPEVISLI-NSRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQ---QDEAYLASLRADQEKERKKREEKERKRRKEEEVQQQKLAEERRRQNLQ-EEKERKLECLPPEPSPDDPESVKIIF--KLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCVPSE-EWPN-------------PPTLQEAGLSHTEVLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Mouse
Match: Faf2 (Fas associated factor family member 2 [Source:MGI Symbol;Acc:MGI:1923827])

HSP 1 Score: 110.538 bits (275), Expect = 2.402e-27
Identity = 53/124 (42.74%), Postives = 77/124 (62.10%), Query Frame = 3 
Query:  258 YGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQL---MEFVKDS 620
            Y V+RP          +N AK  L +LL+YLHGDDH+DSD FCR  LC PE ++ I N  +LFWAC+ N  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+     L   + F+ D+
Sbjct:   53 YVVSRPQ------PRALNDAKRELRFLLVYLHGDDHQDSDEFCRNALCAPEVISLI-NSRMLFWACSTNKPEGYRVSQALRENTYPFLAMIML-KDRRMTVVGRLEGLIQPDDLINQLTFIMDA 168
[Close]
BLAST of FAS-associated factor 2 vs. UniProt/SwissProt
Match: sp|Q28BP9|FAF2_XENTR (FAS-associated factor 2 OS=Xenopus tropicalis OX=8364 GN=faf2 PE=2 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 8.279e-44
Identity = 113/358 (31.56%), Postives = 181/358 (50.56%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQ-----KTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FIQ FE KYG   P+F    Y   +N AK  L +LL+YLHG+DH+DSD FCR TLC PE  NF+ N  +LFWAC+ N  EGF VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   +  N   +   R EREER   ++ +Q+   + E +L     ++  E    E    K++     +  +++EE+ +  L+   +++R S  LP +  + D    V +  K P G+  +  F  T S+ +I+  +F  ++ P +F++  NFP+  + C P++              E   PP   EA L+ + +L+V D +
Sbjct:  108 YTLLDIFRFAVRFIRPDPRSRVTDPVGDVVSFIQLFEEKYGRIHPVFYQGTYSQALNDAKQELRFLLVYLHGEDHQDSDDFCRNTLCIPEVTNFL-NSRMLFWACSTNKPEGFRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPQDLINQLTFIVEANQTYLVSERLEREERNQTQVLRQQ---QDEAYLASLRADQEKERKKKEKQEQKRREEEEAQLKQMLEERKKRNLE--EEKERKSECLPAE-PVPDHPDNVKIIFKMPNGTRVERRFLFTQSLSVIHDFLFSLKETPEKFQIVTNFPRRVLPCLPSE--------------EIPVPPTLQEAGLSQSQLLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. UniProt/SwissProt
Match: sp|Q6AZH6|FAF2A_XENLA (FAS-associated factor 2-A OS=Xenopus laevis OX=8355 GN=faf2-a PE=2 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 3.264e-43
Identity = 113/365 (30.96%), Postives = 173/365 (47.40%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDEC------------ERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FI  FE KYG   P+F    Y   +N AK  L +LL+YLHG+DH+DSD FCR TLC PE  +FI N  +LFWAC+ N  EGF VS+ +RE++YPF+G++ +  + RM +V R EG+     L+  +   +  N   +   R EREER   ++ +Q+  DE              +         R E       +L+++K   L EE           ++R S  LP +  + D    V +  K P G+  +  F  T S+ +I+  +F  ++ P +F++  +FP+  + C P++              E   PP   EA L+ + +L+V D +
Sbjct:  108 YTLLDIFRFTLRFIRPDPRSRVTDPVGDVVSFIHLFEEKYGRIHPVFYQGTYSQALNDAKQELRFLLVYLHGEDHQDSDDFCRNTLCTPEVTHFI-NSRMLFWACSTNKPEGFRVSQALRENTYPFLGMIMLK-DRRMTVVGRLEGLMQPQDLINQLTFIIEANQTYLVSERLEREERNETQVLRQQ-QDEAYLVSLRADQEKERKKKEKQEQKRREEEEAQRKQMLEERKKRNLEEE-----------KERKSECLPAE-PVPDHPDNVKIIFKMPNGTRVERRFLFTQSLSVIHDFLFSLKETPEKFQIVTSFPRRVLPCLPSE--------------EIPVPPTLQEAGLSQSQLLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. UniProt/SwissProt
Match: sp|Q96CS3|FAF2_HUMAN (FAS-associated factor 2 OS=Homo sapiens OX=9606 GN=FAF2 PE=1 SV=2)

HSP 1 Score: 159.458 bits (402), Expect = 5.408e-43
Identity = 116/361 (32.13%), Postives = 182/361 (50.42%), Query Frame = 3 
Query:  135 FPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQ-----KTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            F YY I   F     FI P           D+  F+  FE KYG   P+F    Y   +N AK  L +LL+YLHGDDH+DSD FCR TLC PE ++ I N  +LFWAC+ N  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   M  N   +   R EREER   ++ +Q+   + E +L     ++  E    E    K++     +  KL EE+ +  LQ   K++++  L P       E+ ++I   K P  S  +  F+ + S+ +I+  +F  ++ P +F++  NFP+  + C P++ EW N             PP   EA L+   +L+V D +
Sbjct:  105 FTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLI-NTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQ---QDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQ-EEKERKLECLPPEPSPDDPESVKIIF--KLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCIPSE-EWPN-------------PPTLQEAGLSHTEVLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. UniProt/SwissProt
Match: sp|Q6GQ69|FAF2B_XENLA (FAS-associated factor 2-B OS=Xenopus laevis OX=8355 GN=faf2-b PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 8.421e-43
Identity = 110/359 (30.64%), Postives = 174/359 (48.47%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMK------QKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            Y +   F     FI P           DV  FI  FE KYG   P+F    Y   +N AK  L +LL+YLHG+DH+DSD FCR TLC  E  +FI N  +LFWAC+ N  EGF VS+ + E +YPF+ ++ +  + RM +V R EG+     L+  +   +  N   +   R EREER   ++ +Q+   + E +L     +   +    +    + QK  +  E Q + +L+ R K      ++R S  LP +  + D    V +  K P G+  +  F  T S+ +I+  +F  ++ P +F++  +FP+  + C P++              E   PP   EA L+ + +L+V D +
Sbjct:  108 YTVLDIFRFALRFIRPDPRSRVTDPVGDVVSFIHLFEEKYGSTHPVFYQGTYSQALNDAKQELRFLLVYLHGEDHQDSDDFCRNTLCTSEVTHFI-NSRMLFWACSSNKPEGFRVSQALHESTYPFLAMIMLK-DRRMTVVGRLEGLIQPQDLINQLTFIIEANQTYLVSERLEREERNQTQVLRQQ---QDEAYLVSLRAD---QEKERKKKEKQDQKRREEEEAQRKQMLEERKKRNLEEEKERKSECLPAE-PVPDHPDNVKIIFKMPNGTRVERRFLFTQSLSVIHDFLFSLKETPEKFQIVTSFPRQVLPCLPSE--------------EIPVPPTLQEAGLSQSQLLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. UniProt/SwissProt
Match: sp|Q5BK32|FAF2_RAT (FAS-associated factor 2 OS=Rattus norvegicus OX=10116 GN=Faf2 PE=2 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 2.505e-42
Identity = 113/361 (31.30%), Postives = 181/361 (50.14%), Query Frame = 3 
Query:  135 FPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQ-----KTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            F YY I   F     FI P           D+  F+  FE KYG   P+F    Y   ++ AK  L +LL+YLHGDDH+DSD FCR  LC PE ++ I N  +LFWAC+ N  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+     L+  +   M  N   +   R EREER   ++ +Q+   + E +L     ++  E    E    K++     +  KL EE+ +  LQ   K++++  L P       ++ ++I   K P  S  +  F+ + S+ +I+  +F  ++ P +F++  NFP+  + C P++ EW N             PP   EA L+   +L+V D +
Sbjct:    6 FTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALSDAKRELRFLLVYLHGDDHQDSDEFCRNALCAPEVISLI-NSRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQ---QDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRQNLQ-EEKERKLECLPPEPSPDDPDSVKIIF--KLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCVPSE-EWPN-------------PPTLQEAGLSHTEVLFVQDLT 344
[Close]
BLAST of FAS-associated factor 2 vs. TrEMBL
Match: K1Q3W9 (FAS-associated factor 2-B OS=Crassostrea gigas OX=29159 GN=CGI_10013075 PE=4 SV=1)

HSP 1 Score: 194.126 bits (492), Expect = 6.839e-53
Identity = 121/369 (32.79%), Postives = 189/369 (51.22%), Query Frame = 3 
Query:  114 FLQHLKAFPY-YIYGFFVTIFHFIHPIESLDYESNSTD----VEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISD-ETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 1202
            +L H+  FP+ ++Y   + +  F   +   D   N TD    V  FI  +E   G   P F   +Y   +N AK  L +L +YLHGDDH+D+  FCR TL + + ++FI N  +LFWACN NS EGF VS+ ++E++YPF+ L+ +  N +M +VAR EG    G+L+E ++  + DN   +   RAEREER   +  ++E        L      ER      + I  +KQ+      ++ +++ +    ++ +   +P + ++ D +  R++L  K P GS  +  F    S+K +YY  FC E CP +F V  NFP+  + C+P++                 DPP F EA L  N ML+V D  A
Sbjct:  174 WLYHVIIFPFRFVYSTLLDLVRFTIRLIRPDPRRNVTDPVGDVTRFINSYEETLGTTHPTFYQGSYSQALNDAKRELRFLCVYLHGDDHQDTGDFCRNTLGNQDLIDFI-NTRMLFWACNTNSPEGFRVSRALKENTYPFLALIVLRQN-KMTVVARIEGPIGPGELIEKLERILQDNEASLIAARAEREERDFTQTLRREQDAAYLESLKADQEKERKRREEQDKIDQEKQRLVDEENKRKEMIQERERMKEELKIEIPEEPAVDDPDVVRIVL--KLPHGSRIERRFLKNQSLKFLYYFAFCHEDCPDDFHVVTNFPRRTVPCEPSK--------------NGPDPPSFEEAGLGKNEMLFVQDNEA 524
[Close]
BLAST of FAS-associated factor 2 vs. TrEMBL
Match: A0A210QMY2 (FAS-associated factor 2 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT23485 PE=4 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 3.917e-50
Identity = 110/312 (35.26%), Postives = 169/312 (54.17%), Query Frame = 3 
Query:  135 FPY-YIYGFFVTIFHFIHPIESLDYESNSTD----VEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPK 1055
            FP+ + Y   + I  F+  +   D   N TD    VE F+  +   YG   P F   +Y   +N AK  L +LL+YLHGDDH+D+  FCR TL +P+  +FI N  +LFWACN NS EG+ VS+ +RE++YPF+ LV +  N +M +VAR EG     +L E ++  M DN   +   RAEREER   +  ++E  +     L      ER      E +  +KQK      E+ ++L +  ++++ +   +P + + +D   RV + +K P G   Q  F  +DS+K +Y+  FC E+CP +F +  NFP+
Sbjct:  103 FPFRFAYTSILDILKFVFRLIRPDPRRNVTDPVGDVERFLAKYNELYGDEHPAFYRGSYSQALNDAKRELRFLLVYLHGDDHQDTHSFCRNTLGNPDVRDFI-NGRVLFWACNTNSPEGYRVSRALRENTYPFLALVVLRQN-KMTVVARIEGPVDGQELTEKLERVMTDNETSLIAARAEREERNFTQTLRREQDEAYLESLRADQEKERTRRGDLEKLEQEKQKVVDAEMERQRMLEERELRKEDLRNSIPPEPA-ADHPDRVQIVLKLPHGDRVQRRFLKSDSLKHLYHFAFCHEQCPDDFHIVTNFPR 411
[Close]
BLAST of FAS-associated factor 2 vs. TrEMBL
Match: A0A0B7A409 (UBX domain-containing protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF92545 PE=4 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 1.146e-49
Identity = 119/353 (33.71%), Postives = 185/353 (52.41%), Query Frame = 3 
Query:  147 IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQ-LMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 1202
            ++     +F F  P           DV  FI  FESK+G + P+F +  Y   +N AK  L +LL+YLH +DH+D++ FCR  LC   FVNFI N N+LFWACN +S EG+ VS+ +REH+YPF+ ++ +  N +M +VAR EG  T  Q L++ ++  M++N   +   RAER ER  N+  + +  +     L      E+ +    E + L++ K  +  +E+ +  L+   K+  +  +LP +   +D    V + +K P GS  +  F    SIK ++Y +FC + CP +F++  NFPK  + C+P   E               DPP F E  L  + ML+V D  A
Sbjct:  121 VFSIIRAVFSFFRPDPRRFVTDPVRDVTNFISLFESKFGRSHPVFYHGTYSQALNDAKRELRFLLVYLHSEDHQDTEQFCRDILCSDTFVNFI-NSNMLFWACNTSSPEGYRVSQALREHAYPFLAIIVLRDN-KMSVVARIEGHVTGSQELIDRLEQVMSENESSLVTARAERAERSFNQSLRLQQDEAYLESLRADQEKEKKKREEKEKLELEELKKRQAKKEEEEQFLKKEEKKSAIEAMLPEEPE-ADHPNSVRIVLKIPNGSRIERRFLRNHSIKWLFYYVFCHKDCPDDFQIVTNFPKRTLPCEPKDDE--------------TDPPTFEEIGLGKSEMLFVHDNEA 456
[Close]
BLAST of FAS-associated factor 2 vs. TrEMBL
Match: A0A5K3FK75 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 6.062e-49
Identity = 130/417 (31.18%), Postives = 192/417 (46.04%), Query Frame = 3 
Query:  141 YYIYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKY--GV---------NR-------PMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENL-NILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNG----------RMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHE---------------NILLKKQKTAKLIEEQTQLLLQWRM-KQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFC------------------DPPDFVEANLTSNAMLYVVDCSA 1202
            + +Y F   I  FI P         + DV  FI  F  KY  GV         +R       P F +  Y   + +AK +L +L+IYLHGD HED+D FCR+TL DP  ++F++N   ++FWACN+NS EG+ VS+  REH+YPFIG+V ++ N           RM L+ R EG ++A  L+E++   M D+                  +   E  D  ER L      E+ E+                     + +  + A     + +LL   R+ +Q+R +  LP Q + S +   V + +K P G   Q  F   DS+KL+YY I   E  P++F V  N+PK  I C+PA+ E D +    +P  E C                  DPP F EA L ++ ML ++D  +
Sbjct:   10 HVVYDFIRIILSFIWPDPRRSLTDPAGDVRNFIADFRQKYVPGVLGDQDESETDRVIRESKIPPFFDGTYSEALREAKQSLRFLIIYLHGDSHEDTDSFCRQTLLDPILLSFLDNPEQVIFWACNVNSPEGYRVSQTFREHTYPFIGVVGLSANSAYSRTPYSSTRMALLGRIEGFTSAPILIEYLSKIMNDHQ----------------SILTAERADRAERELSTRLRREQDEAFAASLAQDRAKAAERAASEEAVARLAREAAEARRRDELLGCARLRRQRRWANSLPEQPTPSSDV--VCISLKLPNGRRTQRLFSFKDSLKLLYYFIISQEDAPKQFSVASNYPKRVIECRPAE-ESDIEDY--TPDKEACEDEILCKPVKDWTPNGTTDPPSFEEAGLVNHEMLLILDMDS 405
[Close]
BLAST of FAS-associated factor 2 vs. TrEMBL
Match: A0A147BTL3 (Putative regulator of the ubiquitin pathway (Fragment) OS=Ixodes ricinus OX=34613 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 9.218e-49
Identity = 115/334 (34.43%), Postives = 177/334 (52.99%), Query Frame = 3 
Query:  219 TDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNER-----IESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVI-LRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 1202
             DV  FI+ +ES+YG N P+F    Y   +N AK  L +LLIYLHGDDH+D+  FCR TL + + V FI N ++LFWAC++N  EG+ VS+ +RE++YPF+ ++ +  + RM +V RFEG+     ++  ++  MADN   +   R ER+ER   +  +Q+  +  +  L      ER     +  +  E     +Q+   L+E Q +  +Q RMK + V   +P +   SD  P  I L IK P G+  +  F  + S+K +Y+ +FC  + P  F++  NFP+  + C+P Q              E  +PP F E  L     L+V D  A
Sbjct:  132 ADVMNFIESYESRYGSNHPVFYQGTYSQALNDAKRELKFLLIYLHGDDHQDTGAFCRDTLSNRDLVEFI-NGHMLFWACSVNYPEGYRVSQALRENTYPFLAVIVLREH-RMTVVGRFEGLMRPDVVLLRLQQVMADNEASLVTARMERDERSLTQSIRQQQDEAYQASLLADQEKERRRQEQLRVLEEEQ---SRQRERDLVEVQRKQEIQ-RMKMELVDQ-IPEEPPDSD--PHSIRLVIKLPAGTRLERRFRRSQSLKYLYFYVFCQTEAPDSFEIITNFPRRTLPCEPTQ--------------ECPEPPSFAELGLGKTETLFVHDLDA 442
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Cavefish
Match: faf2 (Fas associated factor family member 2 [Source:NCBI gene;Acc:103021607])

HSP 1 Score: 148.288 bits (373), Expect = 6.990e-40
Identity = 113/377 (29.97%), Postives = 182/377 (48.28%), Query Frame = 3 
Query:   72 LIYSYMFIMRATKVFLQHLKAFPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            L +SY  IM   +        F YY +   F     FI P           DV  FI  FE KYG + P+F    Y   +N AK  L YLL+YLHGDDH+D+D FCR TLC  E + FI N  +LFWAC+ +  EG+ VS+ +RE++YPF+ ++ +  + +M +V R EG+     L+  +   M  N   +   R EREER   ++ +Q+  +     L      ER +    E    +++   + +  + +       +++R S  LP + ++ D+   V +  K P  +  +  F  + S+ +IY  +F  ++ P +F++  NFP+  + C P     D Q           +PP   EA L+ + +L+V D +
Sbjct:   92 LGWSYYLIMLPFR--------FTYYTLLDIFRFALRFIRPDPRGRVTDPVGDVVSFIHSFEEKYGRSHPVFYQGTYSQALNDAKRELRYLLVYLHGDDHQDTDEFCRNTLCTEEVLTFI-NTRMLFWACSTSRPEGYRVSQALRENTYPFLAMIMLK-DRKMTVVGRLEGLIQPEDLINQLTFIMEANQTYLMSERLEREERNQTQVLRQQQDEAYLASLRADQEKERRKREEQEQKRQEEEAVRQSVLAEERRRRTLEEEKERKSKCLPPEPAV-DDPDSVKIVFKLPNDTRVERRFLFSQSLAVIYDFLFSLKETPEKFQIVTNFPRRVLPCLPT----DEQP----------NPPTLKEAGLSRSEVLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Yeast
Match: UBX3 (Clathrin-coated vesicle component, regulator of endocytosis; copurifies with the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; required for efficient clathrin-mediated endocytosis; ortholog of fission yeast Ucp10 [Source:SGD;Acc:S000002249])

HSP 1 Score: 64.6994 bits (156), Expect = 8.117e-12
Identity = 37/132 (28.03%), Postives = 64/132 (48.48%), Query Frame = 3 
Query:  207 ESNSTDVEEFIQY-FESKYGVNRPMFANC----NYVTCVNKAKVTLNYLLIYLHG---DDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEG 578
            +S S +    +Q+ F S Y      F+      +Y   ++     + + +IYLH    D+H D   +  K LC   FVN I    +L W  ++ +SEG  VS  ++   YP +G++++    ++ L+AR EG
Sbjct:  135 DSTSNNESSGVQFSFGSLYNPENGTFSKSIMQNSYTELLDACSEQVKFGVIYLHDPLLDNHMD---YVNKILCSEAFVNMIRKYQVLLWYGDVTTSEGLQVSNALKIRQYPLLGIISLKAEKKIELIARVEG 263
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Nematostella
Match: EDO39604 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9M6])

HSP 1 Score: 136.732 bits (343), Expect = 6.143e-36
Identity = 95/297 (31.99%), Postives = 153/297 (51.52%), Query Frame = 3 
Query:  222 DVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHI---THNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVI-LRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPK----LEIACKPAQLE 1088
            DV  F + FE+KYG   P F   +Y   +N AK  L ++L+YLH DDH+D+  FCR T+ +P F  ++ N N+LFW  +I S EG  VS  +RE +YPF+ L+    N RM +V R EG+ T  Q +  +   + DN   +   R +R+ER   +  + E  ++  R L          R E    +     +++  + I E+ + + + R++++     LP +   S+  P  + +RIK   G   Q  F  T  ++ +Y  +FC E  P EF++  +FP+    LE +C+ A LE
Sbjct:  149 DVLRFKEEFEAKYGTVHPTFYQGSYSQVLNDAKQELRFVLVYLHSDDHQDTPEFCRSTMTNPGFQEYV-NGNMLFWTASIKSPEGSRVSNALRESTYPFLALICRRDN-RMMVVGRMEGLMTVDQYVALLARFIEDNEPALVAARVDRQERSLAQTLRDEQDEDYRRSLQADQEKERRRREEQEKKQKEEEAERRKKQAILEKLESIARLRVEKQ---DQLPDEPDASN--PEALCVRIKLASGKQLQRYFLKTHKLQTLYDFVFCDEDAPTEFRLASHFPRKVYSLE-SCQDATLE 437
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Medaka
Match: faf2 (Fas associated factor family member 2 [Source:NCBI gene;Acc:101159831])

HSP 1 Score: 154.451 bits (389), Expect = 4.345e-42
Identity = 112/377 (29.71%), Postives = 180/377 (47.75%), Query Frame = 3 
Query:   72 LIYSYMFIMRATKVFLQHLKAFPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCS 1199
            L +SY  IM   +        F YY +   F     FI P           DV  FIQ FE KYG + P+F    Y   +N AK  L YLL+YLHGDDH+D+D FCR TLC  E + F+ N  +LFWAC+ +  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+      +  +   M  N   +   R EREER   ++ +Q+  +     L      ER +    E    +++K  +    + +       +++R S  LP +   +D+   V +  K P  +  +  F    S+ +I+  +F  ++ P +F++  NFP+  + C P +              E  +PP   EA L+ + +L+V D +
Sbjct:   92 LGWSYYLIMLPFR--------FTYYTLLDIFRFALRFIRPDPRGRVTDPVGDVVSFIQSFEEKYGRSHPVFYQGTYSQALNDAKRELRYLLVYLHGDDHQDTDEFCRSTLCTEEVITFL-NTQMLFWACSTSKPEGYRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPEDFINQLTFIMDANQTYLMSERLEREERNQTQVLRQQQDEAYLVSLRADQEKERKKREELEQRRQEEEKVRQSALAEERRRRNLEEEKERKSECLPLE-PPADDPESVKIVFKLPNDTRVERRFLFGQSLTVIHDFLFSLKETPEKFQIVTNFPRRVLPCLPTE--------------EQPNPPSLKEAGLSRSEVLFVQDLT 443
[Close]
BLAST of FAS-associated factor 2 vs. Ensembl Medaka
Match: faf2 (Fas associated factor family member 2 [Source:NCBI gene;Acc:101159831])

HSP 1 Score: 147.902 bits (372), Expect = 1.167e-39
Identity = 109/370 (29.46%), Postives = 175/370 (47.30%), Query Frame = 3 
Query:   72 LIYSYMFIMRATKVFLQHLKAFPYY-IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAM 1178
            L +SY  IM   +        F YY +   F     FI P           DV  FIQ FE KYG + P+F    Y   +N AK  L YLL+YLHGDDH+D+D FCR TLC  E + F+ N  +LFWAC+ +  EG+ VS+ +RE++YPF+ ++ +  + RM +V R EG+      +  +   M  N   +   R EREER   ++ +Q+  +     L      ER +    E    +++K  +    + +       +++R S  LP +   +D+   V +  K P  +  +  F    S+ +I+  +F  ++ P +F++  NFP+  + C P +              E  +PP   EA L+ + +
Sbjct:   92 LGWSYYLIMLPFR--------FTYYTLLDIFRFALRFIRPDPRGRVTDPVGDVVSFIQSFEEKYGRSHPVFYQGTYSQALNDAKRELRYLLVYLHGDDHQDTDEFCRSTLCTEEVITFL-NTQMLFWACSTSKPEGYRVSQALRENTYPFLAMIMLK-DRRMTVVGRLEGLIQPEDFINQLTFIMDANQTYLMSERLEREERNQTQVLRQQQDEAYLVSLRADQEKERKKREELEQRRQEEEKVRQSALAEERRRRNLEEEKERKSECLPLE-PPADDPESVKIVFKLPNDTRVERRFLFGQSLTVIHDFLFSLKETPEKFQIVTNFPRRVLPCLPTE--------------EQPNPPSLKEAGLSRSEV 436
[Close]
BLAST of FAS-associated factor 2 vs. Planmine SMEST
Match: SMESG000048881.1 (SMESG000048881.1)

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 3 
Query:  276 MFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 1202
            MFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGVAVCRAEREERCNNRLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA
Sbjct:    1 MFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGVAVCRAEREERCNNRLFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLLQWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 309
[Close]
BLAST of FAS-associated factor 2 vs. Planmine SMEST
Match: SMESG000037279.1 (SMESG000037279.1)

HSP 1 Score: 170.629 bits (431), Expect = 2.961e-48
Identity = 112/371 (30.19%), Postives = 177/371 (47.71%), Query Frame = 3 
Query:  147 IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNR-PMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEG----VSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERF----LHITHNNERIESVG----HENILLKKQKTAKLIEEQTQLLLQWRMKQ----KRVSTLLPCQLSISDETPR--VILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 1202
            I   F+ +F    P        ++ DV+ FIQ  + +   +  P F +  Y  C+  AK  L YL +YLH D+  +     R  LC  EF N I   N+L W C++   EG+ V++ +R+HSYPFIGL+ +  N +M L+ R EG    V    +L + ++D  A                           D  ER     L +  + +  ES+      E IL  K++ A+ I+   +   + R+KQ     R+  L+  +L    E P   +++RI+ P G +++  FY +DS+K++YY +      P  F++  NFPK    C P+ LE +N  IIR PR  F DPP F++  +T+N  + V+D  A
Sbjct:   91 IIEMFMYLFTAFQPDPRHTIIDSAEDVKIFIQQLKLEAAPSELPDFLSQPYGYCIADAKEKLQYLAVYLH-DNTSNCMNTVRNVLCSDEFKNLIREENLLLWGCSVQKPEGYRVAQTLRQHSYPFIGLIGMQNNYKMALLVRVEGEIEKVDIISKLKKVIRDFEAPMVAART--------------------DREERMKTQSLRLQQDMDYAESLRMDREKEKILEDKKERARRIDAIKKEEEEKRVKQIDEMTRIRQLIAAKLPAEPENPENTILIRIQTPSGQLSR-RFYTSDSLKMLYYAVKSQSSAPWNFQITSNFPKKTWPCIPSNLEHENIEIIREPRPNFDDPPSFIDYGITTNITVMVIDQEA 439
[Close]
BLAST of FAS-associated factor 2 vs. Planmine SMEST
Match: SMESG000037279.1 (SMESG000037279.1)

HSP 1 Score: 170.244 bits (430), Expect = 1.038e-47
Identity = 112/371 (30.19%), Postives = 177/371 (47.71%), Query Frame = 3 
Query:  147 IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNR-PMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEG----VSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERF----LHITHNNERIESVG----HENILLKKQKTAKLIEEQTQLLLQWRMKQ----KRVSTLLPCQLSISDETPR--VILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 1202
            I   F+ +F    P        ++ DV+ FIQ  + +   +  P F +  Y  C+  AK  L YL +YLH D+  +     R  LC  EF N I   N+L W C++   EG+ V++ +R+HSYPFIGL+ +  N +M L+ R EG    V    +L + ++D  A                           D  ER     L +  + +  ES+      E IL  K++ A+ I+   +   + R+KQ     R+  L+  +L    E P   +++RI+ P G +++  FY +DS+K++YY +      P  F++  NFPK    C P+ LE +N  IIR PR  F DPP F++  +T+N  + V+D  A
Sbjct:  140 IIEMFMYLFTAFQPDPRHTIIDSAEDVKIFIQQLKLEAAPSELPDFLSQPYGYCIADAKEKLQYLAVYLH-DNTSNCMNTVRNVLCSDEFKNLIREENLLLWGCSVQKPEGYRVAQTLRQHSYPFIGLIGMQNNYKMALLVRVEGEIEKVDIISKLKKVIRDFEAPMVAART--------------------DREERMKTQSLRLQQDMDYAESLRMDREKEKILEDKKERARRIDAIKKEEEEKRVKQIDEMTRIRQLIAAKLPAEPENPENTILIRIQTPSGQLSR-RFYTSDSLKMLYYAVKSQSSAPWNFQITSNFPKKTWPCIPSNLEHENIEIIREPRPNFDDPPSFIDYGITTNITVMVIDQEA 488
[Close]
BLAST of FAS-associated factor 2 vs. Planmine SMEST
Match: SMESG000020232.1 (SMESG000020232.1)

HSP 1 Score: 169.859 bits (429), Expect = 1.263e-47
Identity = 112/371 (30.19%), Postives = 177/371 (47.71%), Query Frame = 3 
Query:  147 IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNR-PMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEG----VSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERF----LHITHNNERIESVG----HENILLKKQKTAKLIEEQTQLLLQWRMKQ----KRVSTLLPCQLSISDETPR--VILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYVVDCSA 1202
            I   F+ +F    P        ++ DV+ FIQ  + +   +  P F +  Y  C+  AK  L YL +YLH D+  +     R  LC  EF N I   N+L W C++   EG+ V++ +R+HSYPFIGL+ +  N +M L+ R EG    V    +L + ++D  A                           D  ER     L +  + +  ES+      E IL  K++ A+ I+   +   + R+KQ     R+  L+  +L    E P   +++RI+ P G +++  FY +DS+K++YY +      P  F++  NFPK    C P+ LE +N  IIR PR  F DPP F++  +T+N  + V+D  A
Sbjct:  140 IIEMFMYLFTAFQPDSRHTIIDSAEDVKIFIQQLKLEAAPSELPDFLSQPYGYCIADAKEKLQYLAVYLH-DNTSNCMNTVRNVLCSDEFKNLIREENLLLWGCSVQKPEGYRVAQTLRQHSYPFIGLIGMQNNYKMALLVRVEGEIEKVDIISKLKKVIRDFEAPMVAART--------------------DREERMKTQSLRLQQDMDYAESLRMDREKEKILEDKKERARRIDVIKKEEEEKRVKQIDEMTRIRQLIAAKLPAEPENPENTILIRIQTPSGQLSR-RFYTSDSLKMLYYAVKSQSSAPWNFQITSNFPKKTWPCIPSNLEHENIEIIREPRPNFDDPPSFIDYGITTNITVMVIDQEA 488
[Close]
BLAST of FAS-associated factor 2 vs. Planmine SMEST
Match: SMESG000020232.1 (SMESG000020232.1)

HSP 1 Score: 164.081 bits (414), Expect = 9.684e-45
Identity = 109/366 (29.78%), Postives = 174/366 (47.54%), Query Frame = 3 
Query:  147 IYGFFVTIFHFIHPIESLDYESNSTDVEEFIQYFESKYGVNR-PMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFCRKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAINTNGRMYLVARFEG----VSTAGQLMEFVKDSMADNSVGXXXXXXXXXXXXXXXLFKQELGDECERF----LHITHNNERIESVG----HENILLKKQKTAKLIEEQTQLLLQWRMKQ----KRVSTLLPCQLSISDETPR--VILRIKFPCGSIAQSNFYLTDSIKLIYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCDPPDFVEANLTSNAMLYV 1187
            I   F+ +F    P        ++ DV+ FIQ  + +   +  P F +  Y  C+  AK  L YL +YLH D+  +     R  LC  EF N I   N+L W C++   EG+ V++ +R+HSYPFIGL+ +  N +M L+ R EG    V    +L + ++D  A                           D  ER     L +  + +  ES+      E IL  K++ A+ I+   +   + R+KQ     R+  L+  +L    E P   +++RI+ P G +++  FY +DS+K++YY +      P  F++  NFPK    C P+ LE +N  IIR PR  F DPP F++  +T+N  + +
Sbjct:  140 IIEMFMYLFTAFQPDSRHTIIDSAEDVKIFIQQLKLEAAPSELPDFLSQPYGYCIADAKEKLQYLAVYLH-DNTSNCMNTVRNVLCSDEFKNLIREENLLLWGCSVQKPEGYRVAQTLRQHSYPFIGLIGMQNNYKMALLVRVEGEIEKVDIISKLKKVIRDFEAPMVAART--------------------DREERMKTQSLRLQQDMDYAESLRMDREKEKILEDKKERARRIDVIKKEEEEKRVKQIDEMTRIRQLIAAKLPAEPENPENTILIRIQTPSGQLSR-RFYTSDSLKMLYYAVKSQSSAPWNFQITSNFPKKTWPCIPSNLEHENIEIIREPRPNFDDPPSFIDYGITTNITVMI 483
The following BLAST results are available for this feature:
BLAST of FAS-associated factor 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
FAF21.126e-4332.13Fas associated factor family member 2 [Source:HGNC... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
H40L08.15.906e-626.97pep chromosome:WBcel235:X:15080779:15084086:-1 gen... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Faf21.589e-2828.47gene:FBgn0025608 transcript:FBtr0343115[more]
Faf21.589e-2828.47gene:FBgn0025608 transcript:FBtr0081126[more]
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BLAST of FAS-associated factor 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
faf25.830e-4028.90Fas associated factor family member 2 [Source:ZFIN... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4
Match NameE-valueIdentityDescription
faf27.890e-4430.70uncharacterized LOC733461 [Source:Xenbase;Acc:XB-G... [more]
faf21.074e-4330.70uncharacterized LOC733461 [Source:Xenbase;Acc:XB-G... [more]
faf21.270e-4331.28uncharacterized LOC733461 [Source:Xenbase;Acc:XB-G... [more]
faf24.375e-4330.70uncharacterized LOC733461 [Source:Xenbase;Acc:XB-G... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Faf23.275e-4331.86Fas associated factor family member 2 [Source:MGI ... [more]
Faf24.226e-4331.86Fas associated factor family member 2 [Source:MGI ... [more]
Faf22.402e-2742.74Fas associated factor family member 2 [Source:MGI ... [more]
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BLAST of FAS-associated factor 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q28BP9|FAF2_XENTR8.279e-4431.56FAS-associated factor 2 OS=Xenopus tropicalis OX=8... [more]
sp|Q6AZH6|FAF2A_XENLA3.264e-4330.96FAS-associated factor 2-A OS=Xenopus laevis OX=835... [more]
sp|Q96CS3|FAF2_HUMAN5.408e-4332.13FAS-associated factor 2 OS=Homo sapiens OX=9606 GN... [more]
sp|Q6GQ69|FAF2B_XENLA8.421e-4330.64FAS-associated factor 2-B OS=Xenopus laevis OX=835... [more]
sp|Q5BK32|FAF2_RAT2.505e-4231.30FAS-associated factor 2 OS=Rattus norvegicus OX=10... [more]
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BLAST of FAS-associated factor 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1Q3W96.839e-5332.79FAS-associated factor 2-B OS=Crassostrea gigas OX=... [more]
A0A210QMY23.917e-5035.26FAS-associated factor 2 OS=Mizuhopecten yessoensis... [more]
A0A0B7A4091.146e-4933.71UBX domain-containing protein (Fragment) OS=Arion ... [more]
A0A5K3FK756.062e-4931.18Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A147BTL39.218e-4934.43Putative regulator of the ubiquitin pathway (Fragm... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
faf26.990e-4029.97Fas associated factor family member 2 [Source:NCBI... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FAS-associated factor 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
UBX38.117e-1228.03Clathrin-coated vesicle component, regulator of en... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO396046.143e-3631.99Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of FAS-associated factor 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
faf24.345e-4229.71Fas associated factor family member 2 [Source:NCBI... [more]
faf21.167e-3929.46Fas associated factor family member 2 [Source:NCBI... [more]
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BLAST of FAS-associated factor 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000048881.10.000e+0100.00SMESG000048881.1[more]
SMESG000037279.12.961e-4830.19SMESG000037279.1[more]
SMESG000037279.11.038e-4730.19SMESG000037279.1[more]
SMESG000020232.11.263e-4730.19SMESG000020232.1[more]
SMESG000020232.19.684e-4529.78SMESG000020232.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003540 ID=SMED30003540|Name=FAS-associated factor 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1234bp
TTTGAATGTATGATATTTTTTTGATTTATTGTTAACGAGAATTTCTAAGA
TATTTTAATATTTAATGAACTCTTATTTATAGTTATATGTTCATTATGAG
AGCTACTAAAGTCTTTCTACAACATTTAAAAGCTTTTCCTTATTATATTT
ACGGATTTTTTGTAACGATTTTTCATTTCATTCATCCTATTGAGTCACTT
GATTATGAAAGTAATTCAACGGATGTTGAAGAATTTATTCAGTATTTTGA
AAGCAAATATGGCGTTAATCGACCAATGTTTGCCAATTGTAATTATGTTA
CTTGCGTTAATAAAGCAAAAGTGACTTTAAATTATTTACTAATCTATCTT
CACGGCGATGATCACGAGGATTCCGATTATTTTTGCAGGAAAACTCTTTG
TGATCCAGAATTTGTGAATTTCATTGAGAATTTGAATATTTTATTCTGGG
CTTGCAATATCAACAGCAGTGAAGGGTTCGGAGTGTCGAAAATTATACGG
GAGCATTCGTATCCATTTATCGGGCTGGTAGCGATCAATACCAACGGTAG
AATGTATTTGGTAGCGAGATTCGAAGGCGTTTCGACAGCCGGTCAGTTGA
TGGAGTTTGTCAAAGACTCCATGGCGGATAATTCGGTTGGTGTGGCTGTT
TGTCGAGCAGAAAGGGAGGAAAGATGTAATAATCGTCTTTTCAAACAAGA
ACTGGGCGATGAATGTGAGCGGTTCCTACACATTACTCATAATAACGAGC
GGATAGAATCCGTTGGTCATGAGAATATTCTTTTAAAAAAACAAAAAACC
GCTAAACTAATAGAGGAACAGACTCAATTATTGTTACAATGGCGGATGAA
ACAGAAACGCGTGAGCACCCTACTTCCATGTCAACTGTCAATTTCAGACG
AGACCCCCCGTGTTATACTGCGGATTAAGTTTCCCTGTGGATCAATTGCC
CAGAGCAATTTCTATTTAACAGATTCAATTAAGCTGATTTATTATACGAT
ATTTTGTTCCGAAAAGTGTCCTCGTGAATTTAAGGTTTACCAGAATTTCC
CGAAGCTTGAAATCGCTTGTAAGCCTGCGCAATTAGAGTGGGACAATCAA
TCAATTATCAGGTCACCGCGAGCCGAATTTTGTGATCCTCCAGATTTTGT
TGAGGCGAATTTGACGTCAAACGCAATGCTGTATGTGGTCGATTGCAGTG
CTTAGCAGGCCCGGGATTGCCGGGAGTCCCCGAG
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protein sequence of SMED30003540-orf-1

>SMED30003540-orf-1 ID=SMED30003540-orf-1|Name=SMED30003540-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=373bp
MFIMRATKVFLQHLKAFPYYIYGFFVTIFHFIHPIESLDYESNSTDVEEF
IQYFESKYGVNRPMFANCNYVTCVNKAKVTLNYLLIYLHGDDHEDSDYFC
RKTLCDPEFVNFIENLNILFWACNINSSEGFGVSKIIREHSYPFIGLVAI
NTNGRMYLVARFEGVSTAGQLMEFVKDSMADNSVGVAVCRAEREERCNNR
LFKQELGDECERFLHITHNNERIESVGHENILLKKQKTAKLIEEQTQLLL
QWRMKQKRVSTLLPCQLSISDETPRVILRIKFPCGSIAQSNFYLTDSIKL
IYYTIFCSEKCPREFKVYQNFPKLEIACKPAQLEWDNQSIIRSPRAEFCD
PPDFVEANLTSNAMLYVVDCSA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002089reproductive organ
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036249Thioredoxin-like_sf
IPR006577UAS
IPR012336Thioredoxin-like_fold
IPR029071Ubiquitin-like_domsf
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005783endoplasmic reticulum
GO:0005811lipid particle
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006577UASSMARTSM0059445neu3coord: 49..176
e-value: 1.6E-23
score: 94.1
IPR036249Thioredoxin-like superfamilyGENE3DG3DSA:3.40.30.10coord: 35..184
e-value: 2.0E-35
score: 123.9
IPR036249Thioredoxin-like superfamilySUPERFAMILYSSF52833Thioredoxin-likecoord: 45..183
NoneNo IPR availableGENE3DG3DSA:3.10.20.90coord: 275..369
e-value: 3.5E-8
score: 35.3
NoneNo IPR availablePANTHERPTHR23322:SF72FAS-ASSOCIATED FACTOR 2coord: 19..326
NoneNo IPR availablePANTHERPTHR23322FAS-ASSOCIATED PROTEINcoord: 19..326
NoneNo IPR availableCDDcd16120UBX_UBXN3Bcoord: 278..369
e-value: 1.85126E-14
score: 66.1418
IPR029071Ubiquitin-like domain superfamilySUPERFAMILYSSF54236Ubiquitin-likecoord: 271..332