SMED30003533
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003533 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30003533 aligns in the following genomic locations:
Homology
BLAST of SMED30003533 vs. Planmine SMEST
Match: SMESG000074376.1 (SMESG000074376.1) HSP 1 Score: 250.366 bits (638), Expect = 4.224e-78 Identity = 118/127 (92.91%), Postives = 124/127 (97.64%), Query Frame = 1 Query: 1144 QARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNYELWCGICQISFTNQNFLDLHLAGFKHFVRLNRGNQAPEGAFGERLIKMRAKLQNLKQSRKAKKSSNLENCQILSNEQSQN 1524 + ++ADI TTVEHCGYCH+SLK+A+DAIAHYQTQKHALNMTNRSRNYELWCGICQISFTNQNFLDLHLAGFKHFVRLNRGNQAPEGAFGERLIKMRAKLQNLKQSRKAKKSSNL NCQILSNEQSQN Sbjct: 219 KTKNADIHTTVEHCGYCHVSLKSANDAIAHYQTQKHALNMTNRSRNYELWCGICQISFTNQNFLDLHLAGFKHFVRLNRGNQAPEGAFGERLIKMRAKLQNLKQSRKAKKSSNLANCQILSNEQSQN 345
BLAST of SMED30003533 vs. Planmine SMEST
Match: SMESG000067725.1 (SMESG000067725.1) HSP 1 Score: 224.557 bits (571), Expect = 3.779e-68 Identity = 104/119 (87.39%), Postives = 112/119 (94.12%), Query Frame = 1 Query: 1144 QARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNYELWCGICQISFTNQNFLDLHLAGFKHFVRLNRGNQAPEGAFGERLIKMRAKLQNLKQSRKAKKSSNLENCQI 1500 +ARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRN+ELWC +CQISFTNQ+FLDLHL GFKHFVR+NRGN APEGAFGERL+KMRAKLQ+LKQSRK KKSSN E Q+ Sbjct: 213 KARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNFELWCDVCQISFTNQSFLDLHLTGFKHFVRVNRGNSAPEGAFGERLMKMRAKLQSLKQSRKEKKSSNDEKRQL 331
BLAST of SMED30003533 vs. Planmine SMEST
Match: SMESG000067725.1 (SMESG000067725.1) HSP 1 Score: 224.557 bits (571), Expect = 4.300e-68 Identity = 104/119 (87.39%), Postives = 112/119 (94.12%), Query Frame = 1 Query: 1144 QARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNYELWCGICQISFTNQNFLDLHLAGFKHFVRLNRGNQAPEGAFGERLIKMRAKLQNLKQSRKAKKSSNLENCQI 1500 +ARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRN+ELWC +CQISFTNQ+FLDLHL GFKHFVR+NRGN APEGAFGERL+KMRAKLQ+LKQSRK KKSSN E Q+ Sbjct: 216 KARSADIPTTVEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNFELWCDVCQISFTNQSFLDLHLTGFKHFVRVNRGNSAPEGAFGERLMKMRAKLQSLKQSRKEKKSSNDEKRQL 334
BLAST of SMED30003533 vs. Planmine SMEST
Match: SMESG000067728.1 (SMESG000067728.1) HSP 1 Score: 68.9366 bits (167), Expect = 1.814e-12 Identity = 33/94 (35.11%), Postives = 55/94 (58.51%), Query Frame = 1 Query: 1174 VEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNYELWCGICQISFTNQNFLDLHLAGFKHFVRLNRGN-QAPEGAFGERLIKMRAKLQNLK 1452 +E CG C SL SDA+ HY ++ H L + + N WC IC+I+ +++ L++HL GF+H+ LNR + G + E+++ R K + L+ Sbjct: 210 MEFCGLCDQSLSGISDAVDHYNSENH-LKLVKNNEN-SCWCNICKITLPDESLLEIHLKGFRHYRILNREECISKTGTYKEKMLYKRMKQKELR 301
BLAST of SMED30003533 vs. Planmine SMEST
Match: SMESG000067728.1 (SMESG000067728.1) HSP 1 Score: 52.7582 bits (125), Expect = 3.125e-7 Identity = 23/61 (37.70%), Postives = 36/61 (59.02%), Query Frame = 1 Query: 1174 VEHCGYCHMSLKNASDAIAHYQTQKHALNMTNRSRNYELWCGICQISFTNQNFLDLHLAGF 1356 +E CG C SL SDA+ HY ++ H L + + N WC IC+I+ +++ L++HL F Sbjct: 210 MEFCGLCDQSLSGISDAVDHYNSENH-LKLVKNNEN-SCWCNICKITLPDESLLEIHLKEF 268 The following BLAST results are available for this feature:
BLAST of SMED30003533 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30003533 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30003533 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30003533 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30003533 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003533 ID=SMED30003533|Name=SMED30003533|organism=Schmidtea mediterranea sexual|type=transcript|length=1709bpback to top protein sequence of SMED30003533-orf-1 >SMED30003533-orf-1 ID=SMED30003533-orf-1|Name=SMED30003533-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=151bp MNDGMMMMMKNNASNKIEFIYKCQARSADIPTTVEHCGYCHMSLKNASDAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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