Threonine synthase-like 2

Overview
NameThreonine synthase-like 2
Smed IDSMED30003523
Length (bp)1653
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Threonine synthase-like 2 (SMED30003523) t-SNE clustered cells

Violin plots show distribution of expression levels for Threonine synthase-like 2 (SMED30003523) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Threonine synthase-like 2 (SMED30003523) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Threonine synthase-like 2 (SMED30003523) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003523

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30003523h1SMcG0021775 SmedASXL_011734SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30003523h1SMcG0021775 Contig41918newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003523h1SMcG0021775 Contig41918uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30003523h1SMcG0021775 dd_Smed_v4_1547_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003523h1SMcG0021775 dd_Smed_v4_1547_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003523h1SMcG0021775 dd_Smed_v4_1547_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30003523h1SMcG0021775 dd_Smed_v6_1547_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003523h1SMcG0021775 dd_Smed_v6_1547_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Threonine synthase-like 2 vs. Ensembl Human
Match: THNSL2 (threonine synthase like 2 [Source:HGNC Symbol;Acc:HGNC:25602])

HSP 1 Score: 255.758 bits (652), Expect = 3.354e-78
Identity = 155/484 (32.02%), Postives = 250/484 (51.65%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFP----LAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDT-----VVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYH---KNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M ++ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSYP L+ E     +       + ++D+    FS+F  + +V + +L++   V+EL+HG T AFKD  L      L Y ++K      +E     K   +++ T+GDTGS+++ES +   + + + VL P    T  Q LQMTT    N       G  DELD+ I+ +  D  F K  N+  LNSIN  R++ QMA H F Y Q    + DT     V + +P G  GNL +  +A ++G+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + V +P ++  K+ S  + S + +D+   +T+  C++E  Y +CPH+A A++Y Y    + +       L  AS  KF EA + AGL    E    +  L+ K+   T++   ++ +  L++ I
Sbjct:    1 MWYVSTRGVAPRVNFEGALFSGYAPDGGLFMPEELPQLDRGTLCQWSTLSYPGLVKELCALFIGSELLPKDELNDLIDRAFSRFRHREVVHLSRLRNGLNVLELWHGVTYAFKDLSLSCTTQFLQYFLEK------RE-----KHVTVVVGTSGDTGSAAIESVQGAKN-MDIIVLLPKGHCTKIQELQMTTVLKQNVHVFGVEGNSDELDEPIKTVFADVAFVKKHNLMSLNSINWSRVLVQMAHHFFAYFQCTPSL-DTHPLPLVEVVVPTGAAGNLAAGYIAQKIGLPIRLVVAVNRNDIIHRTVQQGDFSLSEAVKSTLASAMDIQVPYNMERVFWLLSGSDSQVTRALMEQFERTQSVNLPKELHSKL-SEAVTSVSVSDEAITQTMGRCWDENQYLLCPHSAVAVNYHYQQIDRQQPSTPRCCLAPASAAKFPEAVLAAGLTP--ETPAEIVALEHKETRCTLMRRGDNWMLMLRDTI 468          
BLAST of Threonine synthase-like 2 vs. Ensembl Human
Match: THNSL2 (threonine synthase like 2 [Source:HGNC Symbol;Acc:HGNC:25602])

HSP 1 Score: 210.305 bits (534), Expect = 8.414e-62
Identity = 125/372 (33.60%), Postives = 193/372 (51.88%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFP----LAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDT-----VVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEK 1160
            M ++ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSYP L+ E     +       + ++D+    FS+F  + +V + +L++   V+EL+HG T AFKD  L      L Y ++K      +E     K   +++ T+GDTGS+++ES +     + + VL P    T  Q LQMTT    N       G  DELD+ I+ +  D  F K  N+  LNSIN  R++ QMA H F Y Q    + DT     V + +P G  GNL +  +A ++G+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + V +P ++  K
Sbjct:    1 MWYVSTRGVAPRVNFEGALFSGYAPDGGLFMPEELPQLDRGTLCQWSTLSYPGLVKELCALFIGSELLPKDELNDLIDRAFSRFRHREVVHLSRLRNGLNVLELWHGVTYAFKDLSLSCTTQFLQYFLEK------RE-----KHVTVVVGTSGDTGSAAIESVQ-GAKNMDIIVLLPKGHCTKIQELQMTTVLKQNVHVFGVEGNSDELDEPIKTVFADVAFVKKHNLMSLNSINWSRVLVQMAHHFFAYFQCTPSL-DTHPLPLVEVVVPTGAAGNLAAGYIAQKIGLPIRLVVAVNRNDIIHRTVQQGDFSLSEAVKSTLASAMDIQVPYNMERVFWLLSGSDSQVTRALMEQFERTQSVNLPKELHSK 359          
BLAST of Threonine synthase-like 2 vs. Ensembl Human
Match: THNSL1 (threonine synthase like 1 [Source:HGNC Symbol;Acc:HGNC:26160])

HSP 1 Score: 150.984 bits (380), Expect = 3.681e-38
Identity = 130/455 (28.57%), Postives = 211/455 (46.37%), Query Frame = 3
Query:  120 FSEAILSGLCPDGGLLMP--EFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKND----PVYEHISDISKETFSKFDKNIVEIKKLK-SCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLES-SRFYTD---TLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNI------SCLNSINCGRIICQMALHIFIYLQLAEK----IGDTVVIAIPCGGMGNLISACLANEMGIPIELIVC-TNENDLGNKLIKTGIIEKKETS-HYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQL 1415
            FSEA++ GL  DGGL +P  EF PK +  + +S    +Y       L+  +   D     + E I     E F+     I  ++ L  + F++ELFHGPT +FKD  L  + ++    I               +L+AT+GDTGS+ L   SR   +    + V   FP + ++  Q+ Q+  +  +NG+A+      D     I+ I +D +F+    +      S  NSIN GR++ Q+  H   YL L  +     G  V + IP G  GN+++A  A  MGIPI   +C +N+N +    IKTG  + +E     ++S ++D     NL R L + ++ D        +++E      I   ++EK++   +  + + + E L  I++ +N   Y + PHTA A        +      I  TA   KF+ A ++A LK K  N    +QL
Sbjct:  251 FSEAVIEGLASDGGLFVPAKEF-PKLSCGEWKSLVGATYVERAQILLERCIHPADIPAARLGEMIETAYGENFAC--SKIAPVRHLSGNQFILELFHGPTGSFKDLSLQLMPHIFAHCIPPSCN--------YMILVATSGDTGSAVLNGFSRLNKNDKQRIAVVAFFPENGVSDFQKAQIIGSQRENGWAVGVESDFDFCQTAIKRIFNDSDFTGFLTVEYGTILSSANSINWGRLLPQVVYHASAYLDLVSQGFISFGSPVDVCIPTGNFGNILAAVYAKMMGIPIRKFICASNQNHVLTDFIKTGHYDLRERKLAQTFSPSIDILKSSNLERHLHLMANKDGQLMTELFNRLESQHHFQIEKALVEKLQQDFVADWCS-EGECLAAINSTYNTSGYILDPHTAVAKVVADRVQDKTCPVIISSTAHYSKFAPAIMQA-LKIKEINETSSSQL 692          
BLAST of Threonine synthase-like 2 vs. Ensembl Human
Match: THNSL1 (threonine synthase like 1 [Source:HGNC Symbol;Acc:HGNC:26160])

HSP 1 Score: 150.984 bits (380), Expect = 3.681e-38
Identity = 130/455 (28.57%), Postives = 211/455 (46.37%), Query Frame = 3
Query:  120 FSEAILSGLCPDGGLLMP--EFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKND----PVYEHISDISKETFSKFDKNIVEIKKLK-SCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLES-SRFYTD---TLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNI------SCLNSINCGRIICQMALHIFIYLQLAEK----IGDTVVIAIPCGGMGNLISACLANEMGIPIELIVC-TNENDLGNKLIKTGIIEKKETS-HYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQL 1415
            FSEA++ GL  DGGL +P  EF PK +  + +S    +Y       L+  +   D     + E I     E F+     I  ++ L  + F++ELFHGPT +FKD  L  + ++    I               +L+AT+GDTGS+ L   SR   +    + V   FP + ++  Q+ Q+  +  +NG+A+      D     I+ I +D +F+    +      S  NSIN GR++ Q+  H   YL L  +     G  V + IP G  GN+++A  A  MGIPI   +C +N+N +    IKTG  + +E     ++S ++D     NL R L + ++ D        +++E      I   ++EK++   +  + + + E L  I++ +N   Y + PHTA A        +      I  TA   KF+ A ++A LK K  N    +QL
Sbjct:  251 FSEAVIEGLASDGGLFVPAKEF-PKLSCGEWKSLVGATYVERAQILLERCIHPADIPAARLGEMIETAYGENFAC--SKIAPVRHLSGNQFILELFHGPTGSFKDLSLQLMPHIFAHCIPPSCN--------YMILVATSGDTGSAVLNGFSRLNKNDKQRIAVVAFFPENGVSDFQKAQIIGSQRENGWAVGVESDFDFCQTAIKRIFNDSDFTGFLTVEYGTILSSANSINWGRLLPQVVYHASAYLDLVSQGFISFGSPVDVCIPTGNFGNILAAVYAKMMGIPIRKFICASNQNHVLTDFIKTGHYDLRERKLAQTFSPSIDILKSSNLERHLHLMANKDGQLMTELFNRLESQHHFQIEKALVEKLQQDFVADWCS-EGECLAAINSTYNTSGYILDPHTAVAKVVADRVQDKTCPVIISSTAHYSKFAPAIMQA-LKIKEINETSSSQL 692          
BLAST of Threonine synthase-like 2 vs. Ensembl Human
Match: THNSL2 (threonine synthase like 2 [Source:HGNC Symbol;Acc:HGNC:25602])

HSP 1 Score: 134.035 bits (336), Expect = 1.734e-35
Identity = 71/205 (34.63%), Postives = 108/205 (52.68%), Query Frame = 3
Query:  576 VYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDT-----VVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVI 1175
            + VL P    T  Q LQMTT    N       G  DELD+ I+ +  D  F K  N+  LNSIN  R++ QMA H F Y Q    + DT     V + +P G  GNL +  +A ++G+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + V +P ++  K   V+
Sbjct:    3 IIVLLPKGHCTKIQELQMTTVLKQNVHVFGVEGNSDELDEPIKTVFADVAFVKKHNLMSLNSINWSRVLVQMAHHFFAYFQCTPSL-DTHPLPLVEVVVPTGAAGNLAAGYIAQKIGLPIRLVVAVNRNDIIHRTVQQGDFSLSEAVKSTLASAMDIQVPYNMERVFWLLSGSDSQVTRALMEQFERTQSVNLPKELHSKHSPVL 206          
BLAST of Threonine synthase-like 2 vs. Ensembl Zebrafish
Match: thnsl2 (threonine synthase-like 2 [Source:NCBI gene;Acc:641568])

HSP 1 Score: 249.595 bits (636), Expect = 3.361e-76
Identity = 142/458 (31.00%), Postives = 237/458 (51.75%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFD-KNIVEIKKLK-SCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKI--GDTVVIA---IPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKN-EVGNINAILGTASIEKFSEAGIEAGLKDKFENSI-FLTQLQSK 1424
            M++ ST    +  SF + + SG   DGG+ MPE LP  +   L SW+ LSY  L+ E  +  + + +   E +  +     S F   + V + +LK S  ++ELFHG T AFKD  +   A+ +   ++   +       +  +L+ T+GDTGSS++ S       + + V+FP  RIT  Q LQMTT+  +N       GT D++D  +R +  D +  +   +  LNS+N  RI+ Q A  +F YLQL   +  GDT+ +    +P GG GN+ +  +   MG+P+ L+   N ND+ ++ +++G      +   + + A+D   PYN+ R+ W+ S  D    KS M + ++  K+++P  + +++ S ++ S + +DD  L+ +  C+ + HY ICPHTA A+   Y      G     + TAS  KF +A   AGL  +   S+  L QL+ +
Sbjct:    1 MRYCSTRAGVQGRSFRDVLFSGYAADGGMFMPENLPSLSAETLRSWRRLSYRQLLCEVCELFIPEQEIPREQLEGLVSSALSSFSVPDAVRLVQLKDSLSILELFHGETLAFKDLAMSCTAHFLQYFLRRDRQ-------RATILVGTSGDTGSSAIRSV-LGLREVDIVVVFPRGRITKIQELQMTTSVAENVHVFAADGTSDDIDVPLRKLFADADLVQRHRLMSLNSVNWSRIMVQTAHFLFAYLQLTPSLPEGDTLPVLEVLVPTGGAGNITAGIIVKRMGVPLRLVAMVNANDIVHRTVQSGDFSMSSSVTQTLAPAIDIQDPYNMERVFWLLSGGDGLMVKSLMEEFQKTHKLSLPASLHQQL-SEVLSSGSVSDDGILEAMRRCWQDNHYLICPHTAVAVWRHYQSPVRPGENRCCIATASPHKFQQAVQRAGLTLELPESLQVLDQLEMR 449          
BLAST of Threonine synthase-like 2 vs. Ensembl Zebrafish
Match: thnsl2 (threonine synthase-like 2 [Source:NCBI gene;Acc:641568])

HSP 1 Score: 205.682 bits (522), Expect = 1.114e-60
Identity = 112/369 (30.35%), Postives = 192/369 (52.03%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFD-KNIVEIKKLK-SCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKI--GDTVVIA---IPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKI 1163
            M++ ST    +  SF + + SG   DGG+ MPE LP  +   L SW+ LSY  L+ E  +  + + +   E +  +     S F   + V + +LK S  ++ELFHG T AFKD  +   A+ +   ++   +       +  +L+ T+GDTGSS++ S       + + V+FP  RIT  Q LQMTT+  +N       GT D++D  +R +  D +  +   +  LNS+N  RI+ Q A  +F YLQL   +  GDT+ +    +P GG GN+ +  +   MG+P+ L+   N ND+ ++ +++G      +   + + A+D   PYN+ R+ W+ S  D    KS M + ++  K+++P  + +++
Sbjct:    1 MRYCSTRAGVQGRSFRDVLFSGYAADGGMFMPENLPSLSAETLRSWRRLSYRQLLCEVCELFIPEQEIPREQLEGLVSSALSSFSVPDAVRLVQLKDSLSILELFHGETLAFKDLAMSCTAHFLQYFLRRDRQ-------RATILVGTSGDTGSSAIRSV-LGLREVDIVVVFPRGRITKIQELQMTTSVAENVHVFAADGTSDDIDVPLRKLFADADLVQRHRLMSLNSVNWSRIMVQTAHFLFAYLQLTPSLPEGDTLPVLEVLVPTGGAGNITAGIIVKRMGVPLRLVAMVNANDIVHRTVQSGDFSMSSSVTQTLAPAIDIQDPYNMERVFWLLSGGDGLMVKSLMEEFQKTHKLSLPASLHQQM 361          
BLAST of Threonine synthase-like 2 vs. Ensembl Xenopus
Match: krtcap3 (keratinocyte associated protein 3 [Source:Xenbase;Acc:XB-GENE-5847078])

HSP 1 Score: 255.758 bits (652), Expect = 2.384e-78
Identity = 155/450 (34.44%), Postives = 238/450 (52.89%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAE----KIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINA---ILGTASIEKFSEAGIEAGLKDKFENSI 1400
            MK+ ST        F + + +G  PDGGL MPE +PK     L++W + SY  L+ E     +         +  +  + FS+F  ++IV I KLKS   V+E++HG T AFKD  +  +  L+   +K   + NK I     +L+AT+GDTGSS++ES R   + + + VL P  R T  Q LQMTT   DN       GT DELD  I+ +  D +F K  NI   NS+N  RI+ Q+A   + YLQ       +    V I +P GG GN+ + C+A  MG+PI L+   N ND+ ++ ++ G     +T+  + +SAMD   PYN+ R+LW+ +  D +  K  M + ++ ++V IP ++ ++I  V+  S    DD  L TI  C+ E HY +CPH+A  + Y+++++  GN  +    L  AS  KF +  ++A L       I
Sbjct:    1 MKYTSTRGGLTGVDFEKVLFAGFAPDGGLFMPEEIPKLDKRTLQTWSSYSYIQLVKEICSLFIPPESIPRAELEGLIDKAFSRFRHRDIVPIAKLKSGLNVMEMWHGVTHAFKDLAMSCVGELLGYFLK---RSNKHI----TILVATSGDTGSSAIESVRG-RENIDIVVLLPRGRCTRIQELQMTTVIEDNVHVFAVDGTSDELDYPIKKLFADSDFVKTHNIISTNSVNWARILVQIAHFFYGYLQCVPPTELQCPTPVEIVVPTGGAGNITAGCIAQAMGLPIRLVAVVNRNDIIHRTVQHGDFSLGDTA-ATLASAMDIQEPYNMERILWLLAGSDRSHIKELMEEFQEKKRVKIPEQLHKRIAGVMT-SCRVTDDNILGTIGRCWEENHYLLCPHSAVGV-YYHYQHIDGNQKSPRCCLAPASAAKFQDVIMKANLTPDIPQEI 439          
BLAST of Threonine synthase-like 2 vs. Ensembl Xenopus
Match: slc49a4 (solute carrier family 49 member 4 [Source:Xenbase;Acc:XB-GENE-488397])

HSP 1 Score: 147.517 bits (371), Expect = 7.111e-37
Identity = 131/474 (27.64%), Postives = 221/474 (46.62%), Query Frame = 3
Query:   75 SMKFISTNN------DYEVHSFSEAILSGLCPDGGLLMPE-FLPKFTITQLESWKNLSYPYLMVEFLKFIVDKND-PVYEHISDISKETFSKFD-KNIVEIKKL-KSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTD----TLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFN------ISCLNSINCGRIICQMALHIFIYLQLAEK----IGDTVVIAIPCGGMGNLISACLANEMGIPIELIVC-TNENDLGNKLIKTGIIE-KKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQ 1418
            S  FIST N      + +   FS+A++ GL PDGGL +PE  LPK T  + ++    SY       L+  +   D P +     + K     F    I  ++ L  + F++ELFHGPT +FKD  L  + +L   A  I    N  +M      +AT+GDTGS+ ++     +D    T+ V+ L+P + I+  Q+LQ+     +N   +      D   + I+ +  + +++          +S  NSIN  R++ Q+  H   YL L  +     G  V + IP G  GN+++A  A +MG+PI  ++C +N+N +    +KTG  + + +    S S A+D     N+ R L + +++D  F K    +++      +   ++  I+  II  + +  D  L+ I + +    Y + PHTA A        +      I  TA   KF+ A ++A   D  ++S  L+QL 
Sbjct:  267 SEAFISTRNTSDDARECQTKYFSDAVIEGLAPDGGLYVPEKGLPKLTRGEWKTLVGFSYAERAQIILERCIHPVDVPAFTLGEIVEKAYGENFACHKIAPVRHLVGNQFILELFHGPTASFKDLALQFMPHLF--AYCIPKTCNYLVM------VATSGDTGSAVMDGFGRLSDIDKKTIGVFCLYPENGISQAQKLQIIACQGENSLGLGVGSDFDFCQNTIKKLFTNSDYTGFLTAEYGTVLSTANSINWVRLLPQVVYHASAYLDLVSQGIISFGSHVDVCIPTGNFGNILAALYAKQMGVPIRKLICASNQNHVLTDFLKTGHYDLRGKKLLQSCSPAIDILKSSNIERYLHLLTNNDGKFVKQLFVQLQNEGHFQLRSNLVCLIQEDIIGGWCSEKD-CLEMIHSVYEATGYILDPHTAIAKVVADRVQDRTCPVIISSTAHYSKFAPAILQALRIDSIKHSP-LSQLH 730          
BLAST of Threonine synthase-like 2 vs. Ensembl Mouse
Match: Thnsl2 (threonine synthase-like 2 (bacterial) [Source:MGI Symbol;Acc:MGI:3041254])

HSP 1 Score: 257.684 bits (657), Expect = 3.666e-79
Identity = 149/441 (33.79%), Postives = 234/441 (53.06%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIG----DTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVG--NINAILGTASIEKFSEAGIEAGL 1376
            M + ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSY  L+ E     +         ++D+    FS+F  +N+V + KLK+   ++EL+HG T AFKD  L   A  +   ++ K K          +++ T+GDTGS+++ES +  +  + + VL P    +  Q LQMTT   +N    +  G  DELD+ I+ +  D  F +  N+  LNSIN  R++ QMA H F Y Q    +      TV + +P G  GNL + C+A +MG+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + + +P  +  K+ S  + S +  D+   +T++ C+ E  Y +CPH+ATA++Y Y + + G  +I   L +AS  KF EA   AGL
Sbjct:    1 MWYTSTRGMAPRVNFEGALFSGYAPDGGLYMPEELPRLDEETLRHWSTLSYRSLVKELCALFIGLELIPRHDLNDLIDRAFSRFRHRNVVHLCKLKNGLNILELWHGVTYAFKDLSLSCTAQFLQYFLEKKKKH-------VTIVVGTSGDTGSAAIESVQG-SKNVDIIVLLPKGHCSKIQELQMTTVLKENVHVFEVEGNSDELDEPIKAVFADVAFVQRHNVMSLNSINWSRVLVQMAHHFFAYFQCTPSLDTHPLPTVEVVVPTGAGGNLAAGCIAQKMGLPICLVVAVNRNDIIHRTVQKGDFSLCEVLRTTLASAMDIQVPYNMERIFWLLSGSDSQTTRALMEQFERTQSLQLPKDLHNKL-SEAVTSESVTDEAITQTMARCWEENQYLLCPHSATAVNYHYQQTDSGQSSIRCCLASASAVKFPEAVQAAGL 432          
BLAST of Threonine synthase-like 2 vs. Ensembl Mouse
Match: Thnsl2 (threonine synthase-like 2 (bacterial) [Source:MGI Symbol;Acc:MGI:3041254])

HSP 1 Score: 257.684 bits (657), Expect = 3.666e-79
Identity = 149/441 (33.79%), Postives = 234/441 (53.06%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIG----DTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVG--NINAILGTASIEKFSEAGIEAGL 1376
            M + ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSY  L+ E     +         ++D+    FS+F  +N+V + KLK+   ++EL+HG T AFKD  L   A  +   ++ K K          +++ T+GDTGS+++ES +  +  + + VL P    +  Q LQMTT   +N    +  G  DELD+ I+ +  D  F +  N+  LNSIN  R++ QMA H F Y Q    +      TV + +P G  GNL + C+A +MG+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + + +P  +  K+ S  + S +  D+   +T++ C+ E  Y +CPH+ATA++Y Y + + G  +I   L +AS  KF EA   AGL
Sbjct:    1 MWYTSTRGMAPRVNFEGALFSGYAPDGGLYMPEELPRLDEETLRHWSTLSYRSLVKELCALFIGLELIPRHDLNDLIDRAFSRFRHRNVVHLCKLKNGLNILELWHGVTYAFKDLSLSCTAQFLQYFLEKKKKH-------VTIVVGTSGDTGSAAIESVQG-SKNVDIIVLLPKGHCSKIQELQMTTVLKENVHVFEVEGNSDELDEPIKAVFADVAFVQRHNVMSLNSINWSRVLVQMAHHFFAYFQCTPSLDTHPLPTVEVVVPTGAGGNLAAGCIAQKMGLPICLVVAVNRNDIIHRTVQKGDFSLCEVLRTTLASAMDIQVPYNMERIFWLLSGSDSQTTRALMEQFERTQSLQLPKDLHNKL-SEAVTSESVTDEAITQTMARCWEENQYLLCPHSATAVNYHYQQTDSGQSSIRCCLASASAVKFPEAVQAAGL 432          
BLAST of Threonine synthase-like 2 vs. Ensembl Mouse
Match: Thnsl1 (threonine synthase-like 1 (bacterial) [Source:MGI Symbol;Acc:MGI:2139347])

HSP 1 Score: 144.821 bits (364), Expect = 3.090e-36
Identity = 123/447 (27.52%), Postives = 208/447 (46.53%), Query Frame = 3
Query:  120 FSEAILSGLCPDGGLLMPEF-LPKFTITQLESWKNL---SYPYLMVEFLKFIVDKND----PVYEHISDISKETFSKFDKNIVEIKKLK-SCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLES----SRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNI------SCLNSINCGRIICQMALHIFIYLQLAEK----IGDTVVIAIPCGGMGNLISACLANEMGIPIELIVC-TNENDLGNKLIKTGIIE-KKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEA-GLKD 1382
            FSEA++ GL  DGGL +PE   PK +  +   W NL   +Y       L+  +   D     + E I     E F+     +  ++ L  + F++ELF+GPT +FKD  L  + ++    I               +L+AT+GDTGS+ L      ++   + + V   FP + ++  Q+ ++  +  +NG+AI      D     IR I +D +F+    +      S  NSIN  R++ Q+  H   YL+L  +     G  V + +P G  GN+++A  A  MGIPI   +C +N+N +    IKTG  + +      ++S ++D     NL R L++ ++ D     +   ++E      I   ++EK++   +  + + + E L  IST +N   Y + PHTA A        +      I  TA   KF+ A ++A G+K+
Sbjct:  251 FSEAVVEGLASDGGLFVPEKEFPKLSPGE---WNNLIGATYIERAQVLLERCIHPADIPAAKLGEMIETAYGENFAC--SKVAPVRHLSGNQFILELFYGPTGSFKDLSLQLMPHIFAYCIPPGCN--------YVILVATSGDTGSAVLNGFSHLNKNDKERIAVVTFFPENGVSDFQKAEIIGSQRENGWAIGVRSDFDFCQTAIRKIFNDSDFTGFLAVEYGTILSSANSINWARLLPQIVYHASAYLELVNQRFISFGSPVDVCVPTGNFGNVLAAVYAKMMGIPIRKFICASNQNHVLTDFIKTGHYDLRNRKLAQTFSPSIDILKSSNLERHLYLMANKDGQLMANLYHQLESQLHFRIEKMLVEKLQQEFVADWCS-EGECLAAISTTYNASGYILDPHTAVAKVVADKMQDKSCPVLIASTAHYSKFAPAIMQALGIKE 683          
BLAST of Threonine synthase-like 2 vs. Ensembl Mouse
Match: Thnsl2 (threonine synthase-like 2 (bacterial) [Source:MGI Symbol;Acc:MGI:3041254])

HSP 1 Score: 68.9366 bits (167), Expect = 2.281e-13
Identity = 40/148 (27.03%), Postives = 68/148 (45.95%), Query Frame = 3
Query:  939 NENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVG--NINAILGTASIEKFSEAGIEAGL 1376
            N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + + +P  ++                                 CPH+ATA++Y Y + + G  +I   L +AS  KF EA   AGL
Sbjct:    2 NRNDIIHRTVQKGDFSLCEVLRTTLASAMDIQVPYNMERIFWLLSGSDSQTTRALMEQFERTQSLQLPKDLL---------------------------------CPHSATAVNYHYQQTDSGQSSIRCCLASASAVKFPEAVQAAGL 116          
BLAST of Threonine synthase-like 2 vs. Ensembl Mouse
Match: Thnsl1 (threonine synthase-like 1 (bacterial) [Source:MGI Symbol;Acc:MGI:2139347])

HSP 1 Score: 54.299 bits (129), Expect = 8.617e-8
Identity = 44/162 (27.16%), Postives = 77/162 (47.53%), Query Frame = 3
Query:  906 MGIPIELIVC-TNENDLGNKLIKTGIIE-KKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEA-GLKD 1382
            MGIPI   +C +N+N +    IKTG  + +      ++S ++D     NL R L++ ++ D     +   ++E      I   ++EK++   +  + + + E L  IST +N   Y + PHTA A        +      I  TA   KF+ A ++A G+K+
Sbjct:    2 MGIPIRKFICASNQNHVLTDFIKTGHYDLRNRKLAQTFSPSIDILKSSNLERHLYLMANKDGQLMANLYHQLESQLHFRIEKMLVEKLQQEFVADWCS-EGECLAAISTTYNASGYILDPHTAVAKVVADKMQDKSCPVLIASTAHYSKFAPAIMQALGIKE 162          
BLAST of Threonine synthase-like 2 vs. UniProt/SwissProt
Match: sp|Q5M7T9|THNS2_RAT (Threonine synthase-like 2 OS=Rattus norvegicus OX=10116 GN=Thnsl2 PE=2 SV=1)

HSP 1 Score: 258.07 bits (658), Expect = 2.406e-78
Identity = 156/480 (32.50%), Postives = 248/480 (51.67%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQ----LAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAI----LGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M + ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSY  L+ E     V         ++ +  + FS+F  +++V + KLK+   ++EL+HG T AFKD  L   A  +   ++ K K          +++ T+GDTGS+++ES +  +  + + VL P    +  Q LQMTT   +N       G  DELD+ I+ +  D  F +  N+  LNSIN  R++ QMA H F Y Q    L   +  TV + +P G  GNL + C+A +MG+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ +  D+  T++ M + E+ + + +P  +  K+ S  + S + +D+   +T+  C+ E  Y +CPH+ATA+SY Y + + G  ++I    L  AS  KF EA   AGL  +    I    L+ K+   T +   +D    L++ I
Sbjct:    1 MWYTSTRGMAPRVNFEGALFSGYAPDGGLYMPEELPRLDKETLRHWSTLSYRSLVKELCALFVGLELIPRHDLNGLIDQAFSRFRHRDVVHLCKLKNGLNILELWHGVTYAFKDLSLSCTAQFLQYFLEKKKKH-------VTIVVGTSGDTGSAAIESVQG-SKNVDIIVLLPKGHCSKIQELQMTTVVKENVHVFGVEGNSDELDEPIKAVFADVAFVQRHNLMSLNSINWSRVLVQMAHHFFAYFQCTPSLDMHLLPTVEVVVPTGAGGNLAAGCIAQKMGLPISLVVAVNRNDIIHRTVQKGDFSLCEVVRKTLASAMDIQVPYNMERIFWLLAGSDSQTTRALMEQFERTQSLHLPKDLHSKL-SEAVTSESVSDEAITQTMGRCWEENQYLLCPHSATAVSYHYQQTDSGQPSSIPRCCLAPASAVKFPEAVQAAGLTPETPAEIL--ALEHKETRCTPMRRGDDWTQMLRDTI 469          
BLAST of Threonine synthase-like 2 vs. UniProt/SwissProt
Match: sp|Q80W22|THNS2_MOUSE (Threonine synthase-like 2 OS=Mus musculus OX=10090 GN=Thnsl2 PE=1 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 2.564e-78
Identity = 149/441 (33.79%), Postives = 234/441 (53.06%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIG----DTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVG--NINAILGTASIEKFSEAGIEAGL 1376
            M + ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSY  L+ E     +         ++D+    FS+F  +N+V + KLK+   ++EL+HG T AFKD  L   A  +   ++ K K          +++ T+GDTGS+++ES +  +  + + VL P    +  Q LQMTT   +N    +  G  DELD+ I+ +  D  F +  N+  LNSIN  R++ QMA H F Y Q    +      TV + +P G  GNL + C+A +MG+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + + +P  +  K+ S  + S +  D+   +T++ C+ E  Y +CPH+ATA++Y Y + + G  +I   L +AS  KF EA   AGL
Sbjct:    1 MWYTSTRGMAPRVNFEGALFSGYAPDGGLYMPEELPRLDEETLRHWSTLSYRSLVKELCALFIGLELIPRHDLNDLIDRAFSRFRHRNVVHLCKLKNGLNILELWHGVTYAFKDLSLSCTAQFLQYFLEKKKKH-------VTIVVGTSGDTGSAAIESVQG-SKNVDIIVLLPKGHCSKIQELQMTTVLKENVHVFEVEGNSDELDEPIKAVFADVAFVQRHNVMSLNSINWSRVLVQMAHHFFAYFQCTPSLDTHPLPTVEVVVPTGAGGNLAAGCIAQKMGLPICLVVAVNRNDIIHRTVQKGDFSLCEVLRTTLASAMDIQVPYNMERIFWLLSGSDSQTTRALMEQFERTQSLQLPKDLHNKL-SEAVTSESVTDEAITQTMARCWEENQYLLCPHSATAVNYHYQQTDSGQSSIRCCLASASAVKFPEAVQAAGL 432          
BLAST of Threonine synthase-like 2 vs. UniProt/SwissProt
Match: sp|Q5XH07|THNS2_XENLA (Threonine synthase-like 2 OS=Xenopus laevis OX=8355 GN=thnsl2 PE=2 SV=1)

HSP 1 Score: 255.758 bits (652), Expect = 1.275e-77
Identity = 160/475 (33.68%), Postives = 246/475 (51.79%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVV-IAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINA--ILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            MK+ ST        F   + SG  PDGGL MPE +PK     L++W + SY  L+ E     +         +  +    F +F  ++IV I +LKS   V+E++HG T AFKD  +  +  L+D  +K   ++NK +     +L+AT+GDTGSS++ES R   + + + VL P+ R T  Q LQMTT   DN       GT DELD  I+ +  D +F K  NI   NS+N  RI+ Q+A   + Y+Q A     T V I +P GG GN+ + C+A  MG+PI L+   N ND+ ++ ++ G     +T   + +SAMD   PYN+ R+LW+ +  + +  K  M + ++ ++V +P ++ +KI   +  S    D+  L TI  C+ E HY +CPH+A A+ Y Y + +  + +    L  AS  KF +  I+A L       I    L+ KK     L + +D    L+  I
Sbjct:    1 MKYTSTRGGLIGVDFEGVLFSGFAPDGGLFMPEDIPKVDKRTLQTWSSYSYIQLVKEICSLFISPESIPRADLEGLIDRAFIRFRHRDIVPITRLKSGLNVMEMWHGVTHAFKDLAMSCVGELLDYFLK---RKNKHV----TILVATSGDTGSSAIESVR-RRENMDIIVLLPHGRCTKIQELQMTTVIEDNVHVFSVDGTSDELDYPIKRLFADSDFVKKHNIMSTNSVNWARILVQIAHFFYGYMQCAPLTELTPVEIIVPTGGAGNITAGCIAQAMGLPIHLVAVVNRNDIVHRTVQYGDFSLGDTK-ATLASAMDIQEPYNMERILWLLAGSEKSHIKEMMKEFQEKKRVKLPEQLHKKIAGAMT-SCVVTDENILGTIGRCWEENHYLLCPHSAVAVYYHYQQMDSNDKSPRCCLAPASAAKFQDVIIKANLTPDIPQEI--KDLEKKKTRSHHLTKEDDWEKVLRQTI 463          
BLAST of Threonine synthase-like 2 vs. UniProt/SwissProt
Match: sp|Q86YJ6|THNS2_HUMAN (Threonine synthase-like 2 OS=Homo sapiens OX=9606 GN=THNSL2 PE=2 SV=3)

HSP 1 Score: 255.758 bits (652), Expect = 1.611e-77
Identity = 155/484 (32.02%), Postives = 250/484 (51.65%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFP----LAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDT-----VVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYH---KNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M ++ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSYP L+ E     +       + ++D+    FS+F  + +V + +L++   V+EL+HG T AFKD  L      L Y ++K      +E     K   +++ T+GDTGS+++ES +   + + + VL P    T  Q LQMTT    N       G  DELD+ I+ +  D  F K  N+  LNSIN  R++ QMA H F Y Q    + DT     V + +P G  GNL +  +A ++G+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + V +P ++  K+ S  + S + +D+   +T+  C++E  Y +CPH+A A++Y Y    + +       L  AS  KF EA + AGL    E    +  L+ K+   T++   ++ +  L++ I
Sbjct:    1 MWYVSTRGVAPRVNFEGALFSGYAPDGGLFMPEELPQLDRGTLCQWSTLSYPGLVKELCALFIGSELLPKDELNDLIDRAFSRFRHREVVHLSRLRNGLNVLELWHGVTYAFKDLSLSCTTQFLQYFLEK------RE-----KHVTVVVGTSGDTGSAAIESVQGAKN-MDIIVLLPKGHCTKIQELQMTTVLKQNVHVFGVEGNSDELDEPIKTVFADVAFVKKHNLMSLNSINWSRVLVQMAHHFFAYFQCTPSL-DTHPLPLVEVVVPTGAAGNLAAGYIAQKIGLPIRLVVAVNRNDIIHRTVQQGDFSLSEAVKSTLASAMDIQVPYNMERVFWLLSGSDSQVTRALMEQFERTQSVNLPKELHSKL-SEAVTSVSVSDEAITQTMGRCWDENQYLLCPHSAVAVNYHYQQIDRQQPSTPRCCLAPASAAKFPEAVLAAGLTP--ETPAEIVALEHKETRCTLMRRGDNWMLMLRDTI 468          
BLAST of Threonine synthase-like 2 vs. UniProt/SwissProt
Match: sp|Q5RFE6|THNS2_PONAB (Threonine synthase-like 2 OS=Pongo abelii OX=9601 GN=THNSL2 PE=2 SV=1)

HSP 1 Score: 254.988 bits (650), Expect = 3.009e-77
Identity = 151/480 (31.46%), Postives = 247/480 (51.46%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDT-----VVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYH---KNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M ++ST       +F  A+ SG  PDGGL MPE LP+     L  W  LSYP L+ E     +       + ++D+    FS+F  + +V + +L++   V+EL+HG T AFKD  L   A  +   ++ + K          +++ T+GDTGS+++ES +     + + VL P    T  Q LQMTT   +N       G  DELD+ I+ +  D  F K  N+  LNSIN  R++ QMA H F Y Q    + DT     V + +P G  GNL +  +A ++G+P+ L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  D+  T++ M + E+ + V +P ++  K+ S  + S + +D+   +T+  C++E  Y +CPH+A A++Y Y    + +       L  AS  KF EA + AGL    E    +  L+ K+   T +   ++ +  L++ I
Sbjct:    1 MWYVSTRGIAPRVNFEGALFSGYAPDGGLFMPEELPQLDRKTLCQWSTLSYPGLVKELCALFIGSELIPKDELNDLIDRAFSRFRHREVVHLSRLRNGLNVLELWHGVTYAFKDLSLSCTAQFLQYFLEKREKH-------VTVVVGTSGDTGSAAIESVQG-AKNMDIIVLLPKGHCTKIQELQMTTVLKENVHVFGVEGNSDELDEPIKTVFADVAFVKKHNLMSLNSINWSRVLVQMAHHFFAYFQCMPSL-DTHPLPLVEVVVPTGAAGNLAAGYIAQKIGLPVRLVVAVNGNDIIHRTVQQGDFSLSEAVKSTLASAMDIQVPYNMERVFWLLSGSDSQVTRALMEQFERTQSVNLPKELHSKL-SEAVTSVSVSDEAITQTMGRCWDENQYLLCPHSAVAVNYHYQQMDRQQPSTPRCCLAPASAAKFPEAVLAAGLTP--ETPAEIVALEHKETRCTPMRRGDNWMLMLRDTI 468          
BLAST of Threonine synthase-like 2 vs. TrEMBL
Match: A0A1S3KBX2 (threonine synthase-like 2 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106180649 PE=4 SV=1)

HSP 1 Score: 275.404 bits (703), Expect = 2.305e-82
Identity = 165/492 (33.54%), Postives = 261/492 (53.05%), Query Frame = 3
Query:   27 SITNSPGLLFSLIVELSMKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYH---KNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            +I+ SP + ++     +M++ S+       +F EA+ SG   DGG+LMP+ +PK T  Q+  W  LSY  L+ E +   V + +   E  S +  + FS+F   ++V I KLK+   ++ELFHG T AFKD  +  +    +  +K K +          +L+ T+GDTGS+++E+ R   + + + V+ P  R T  Q LQMTT   DN    +  GT DE+D+ ++    D  F++  N+  +NSIN  RI+ Q+A + + Y QL     DTV IA+P GG GN+ + CLA  MG+PIE+ V  N ND+   +   G ++   T   S + AMD   PYN+ R+L++ S+ +    K  M   E G +  IP  + +K++ ++  S+ A+DD     ++ C+ E  Y +CPHTA A +Y Y+    ++V      + TAS  KF EA   AGL  +    I    L++K   +  +N+ ED    LK  I
Sbjct:   20 AISKSPRMAYT-----NMRYNSSRGQINDFTFEEALFSGFTSDGGMLMPDEIPKVTSDQIRHWSKLSYTELVKEVVALFVPEEEVPREDQSSLIDKAFSRFTHPDVVNIAKLKNGLNILELFHGTTLAFKDLAMSCVGQFFNYFLKKKERH-------MVVLVGTSGDTGSAAIEAMRGL-EAVDIVVMLPKGRCTRIQELQMTTVIEDNVHVYRVDGTSDEIDEPLKNCFTDAAFAQENNLCSVNSINWCRIMVQIAHYFYAYFQLCPGCDDTVEIAVPTGGCGNITAGCLAWFMGLPIEITVTVNRNDIMYHICTEGKLKMPSTMTPSLAPAMDIQNPYNMQRILYMLSNSNGKLVKEIMETFEAGREAVIPDDVFKKMKKIVTKSFVADDDLIKSAMARCWKENQYLLCPHTAVAAAYHYNTLVNSDVIPKRVCIATASPAKFPEAVTLAGLTPQPTPEI--AALETKPTRYIDMNKGEDWTGILKEKI 496          
BLAST of Threonine synthase-like 2 vs. TrEMBL
Match: A0A099ZC60 (Threonine synthase-like 2 (Fragment) OS=Tinamus guttatus OX=94827 GN=N309_10227 PE=4 SV=1)

HSP 1 Score: 274.248 bits (700), Expect = 3.561e-82
Identity = 160/480 (33.33%), Postives = 253/480 (52.71%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDT----VVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINA---ILGTASIEKFSEAGIEAGLKDKFENSI-FLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M+++ST        F EA+ SG  PDGGL MP  +P+     L SW + SYP L+ E     V         + D+    FS+F  K++V + +LK    V+EL+HG T AFKD  L      +   ++ K        K  ++L+ T+GDTGSS++ES R   + + ++VL P+   T TQ LQMTT   +N       G  DE+D+ I+ +  D +F++  N+  LNS+N  RI+ Q+A H + Y Q A  +  +    V I +P GG GN+ + C+A +MG+PI L+   N ND+ ++ ++ G      T   + +SAMD   PYN+ R+LW+ SD D++  K+ M +    + + +P  +  K+ +  +DS AA+D++ ++ +  C+ E  Y +CPH+A A  Y Y +   G+ +     L  AS  KF +A + AGL       I  LT L+++    T L + ED    L+  I
Sbjct:    1 MEYVSTRGGPGTVGFEEALFSGYAPDGGLFMPSAVPRLEAHTLRSWSSFSYPRLVKELCALFVAAELIPRSVLEDLIDRAFSRFRHKDVVHLTRLKDGLNVLELWHGATYAFKDLSLSCTGQFLQYFLEKK-------QKHLSILVGTSGDTGSSAIESVRGQKN-MDIFVLLPHGLCTQTQELQMTTVIEENVHVFDVHGNSDEIDEPIKELFADTDFARKHNLMSLNSVNWSRIMVQIAHHFYAYFQCAPSLDTSPLPMVEIVVPTGGGGNITAGCIAQKMGLPIRLVAVVNSNDIIHRAVQHGDFSLSGTVKATLASAMDIQEPYNMERILWLLSDGDSSLIKTLMEQFSVSKSLKLPENLHRKL-AQTLDSCAASDEDIMRAMRRCWEENRYLLCPHSAVAAHYHYAQLGCGSRSTPRCCLAPASAAKFQDALLRAGLVPDVPPEISALTTLETRS---TPLKKGEDWAKVLRERI 468          
BLAST of Threonine synthase-like 2 vs. TrEMBL
Match: K1PM14 (Threonine synthase-like 2 OS=Crassostrea gigas OX=29159 GN=CGI_10007336 PE=4 SV=1)

HSP 1 Score: 273.863 bits (699), Expect = 5.236e-82
Identity = 154/476 (32.35%), Postives = 254/476 (53.36%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFD-KNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHK----NEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            MK+ ST  D    +F EA+ +G   DGGL++PE +PK    +L SW  LSY  L  + +   VD+++     +SDI  + FS+F    +  IK+L     V+ELFHGPT +FKD  L  +   I   +  + K         N+++ T+GDTGSS++E+ R     + + VL P  R +  Q LQMTT   DN    +  G+ D+LD  I+    D  F+K  N+S +NSIN  RI+ Q   +I+ YLQ+ +     V + +P G +GN+ S  +A EMG+ +E +    +ND+  + I+TG     +    + + AMD  +PYN+ R+ ++ +  D    K+ M++ E+   V +P +I E ++ V++D++ A DD   +T+   ++   Y +CPHTAT ++Y Y +     E      I+ TAS+ KF EA   A +   F     +++L   K+ +  +N+ ++ +  L+  I
Sbjct:    1 MKYKSTRGDVTGQTFEEALFTGYAADGGLILPETIPKIDAKELTSWAKLSYVELAKKIVPLFVDEDEIPTSDLSDIIDKAFSRFSVPEVSPIKRLPDGLNVMELFHGPTWSFKDLALSCVGQFIPYFLSKRKKHF-------NIIVGTSGDTGSSAIEAVRG-QKWIDIVVLLPKGRCSQIQELQMTTVIEDNVHVYRVDGSSDDLDAPIKLCFRDNAFTKRHNLSSINSINWARIMVQTVHYIYAYLQMCQSCDKVVQVVVPTGALGNITSGIIAREMGVQLEFVCAVTQNDVVARTIQTGNYAIADHVIETLAPAMDIQIPYNMERIWYVLTGGDCKLIKNLMTEFEETGNVMVPKEITEALQKVVVDTFVATDDVIRRTMKRTWDAHEYLVCPHTATGVAYHYQQLQKNPEEKKARIIIATASVIKFEEALQSAAI--PFTRDPRVSKLFESKQRYNDMNKEDNWLEMLQEKI 466          
BLAST of Threonine synthase-like 2 vs. TrEMBL
Match: A0A1S3KD04 (threonine synthase-like 2 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106180649 PE=4 SV=1)

HSP 1 Score: 272.707 bits (696), Expect = 1.073e-81
Identity = 162/475 (34.11%), Postives = 252/475 (53.05%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYH---KNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M++ S+       +F EA+ SG   DGG+LMP+ +PK T  Q+  W  LSY  L+ E +   V + +   E  S +  + FS+F   ++V I KLK+   ++ELFHG T AFKD  +  +    +  +K K +          +L+ T+GDTGS+++E+ R   + + + V+ P  R T  Q LQMTT   DN    +  GT DE+D+ ++    D  F++  N+  +NSIN  RI+ Q+A + + Y QL     DTV IA+P GG GN+ + CLA  MG+PIE+ V  N ND+   +   G ++   T   S + AMD   PYN+ R+L++ S+ +    K  M   E G +  IP  + +K++ ++  S+ A+DD     ++ C+ E  Y +CPHTA A +Y Y+    ++V      + TAS  KF EA   AGL  +    I    L++K   +  +N+ ED    LK  I
Sbjct:    1 MRYNSSRGQINDFTFEEALFSGFTSDGGMLMPDEIPKVTSDQIRHWSKLSYTELVKEVVALFVPEEEVPREDQSSLIDKAFSRFTHPDVVNIAKLKNGLNILELFHGTTLAFKDLAMSCVGQFFNYFLKKKERH-------MVVLVGTSGDTGSAAIEAMRGL-EAVDIVVMLPKGRCTRIQELQMTTVIEDNVHVYRVDGTSDEIDEPLKNCFTDAAFAQENNLCSVNSINWCRIMVQIAHYFYAYFQLCPGCDDTVEIAVPTGGCGNITAGCLAWFMGLPIEITVTVNRNDIMYHICTEGKLKMPSTMTPSLAPAMDIQNPYNMQRILYMLSNSNGKLVKEIMETFEAGREAVIPDDVFKKMKKIVTKSFVADDDLIKSAMARCWKENQYLLCPHTAVAAAYHYNTLVNSDVIPKRVCIATASPAKFPEAVTLAGLTPQPTPEI--AALETKPTRYIDMNKGEDWTGILKEKI 465          
BLAST of Threonine synthase-like 2 vs. TrEMBL
Match: A0A485PR69 (Threonine synthase-like 2-like OS=Lynx pardinus OX=191816 GN=LYPA_23C014884 PE=4 SV=1)

HSP 1 Score: 272.322 bits (695), Expect = 2.314e-81
Identity = 159/479 (33.19%), Postives = 250/479 (52.19%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKF-DKNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIG----DTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNIN---AILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M++IST        F  A+ SG  PDGGL MPE LP+ +I  L  W  LSYP L+ E     +       + ++ +  + FS+F  + +V + +L++   V+EL+HG T AFKD  L   A  +   ++ K K          +++ T+GDTGS+++ES +     + + VL P    T  Q LQMTT   +N       G  DELD+ I+ +  D  F K  N+  LNSIN  R++ QMA H F Y Q A  +       V + +P G  GNL + C+A +MG+PI L+V  N ND+ ++ ++ G     E    + +SAMD  +PYN+ R+ W+ S  ++  T++ M + E+ + V++P ++  K+ S  + S + +D    +T++ C+ E  Y +CPH+A A+SY Y + E    +     L  AS  KF EA + AGL  +    I    L+SK    T + + +D    L+N I
Sbjct:    1 MRYISTRGMAPRVDFEGALFSGYAPDGGLFMPEELPQLSIETLRQWSTLSYPGLVKELCTLFIGPELIPRDVLNGLIDQAFSRFRHREVVHLSRLRNGLNVLELWHGVTYAFKDLSLCCTAQFLQYFLEKKEKH-------VTVVVGTSGDTGSAAIESVQ-GAKNMDIIVLLPKGHCTKIQELQMTTVLRENVHVFGVEGNSDELDEPIKAVFADVAFVKKHNLMSLNSINWSRVLVQMAHHFFAYFQCASSLDMDPLPLVEVVVPTGAAGNLAAGCIAQKMGLPIRLVVAVNRNDIIHRTVQQGDFSLSEAVEQTLASAMDIQVPYNVERIFWLLSGSNSQVTRALMEQFERTQSVSLPKELHSKL-SGAVTSESVSDKAITQTMARCWEENKYLLCPHSAVAVSYHYQQTEQQPPSLPRCCLAPASAAKFPEAVLAAGLTPETPAEIL--ALESKGTRCTPMRKGDDWTLMLRNTI 468          
BLAST of Threonine synthase-like 2 vs. Ensembl Cavefish
Match: thnsl2 (threonine synthase-like 2 [Source:NCBI gene;Acc:641568])

HSP 1 Score: 192.586 bits (488), Expect = 5.391e-55
Identity = 139/474 (29.32%), Postives = 223/474 (47.05%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLK-FIVDKNDPVYEHISDISKETFSKFD-KNIVEIKKLK-SCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYH-KNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFI 1487
            M++ ST  +    SF + + SG  PDGG+ MPE +P      L SW +LSY  L+ E    FI ++  P ++  + IS    SKF   + V + KLK S  ++ELFHG T AFKD  +      ++  +   +KE+    +   +L+ T+GDTG S++ SS    + + V V+FP  RIT  Q  QMTT   DN       G+ DE+D  +R +  D +  K      L S+N G ++   A                                C+   MGIP+ L+   N ND+ ++ ++ G     ++   + + A+D   PYN+ R+ W+ S  D    K  M + ++  ++T+P  + +++ SV + S + +D+  ++ +  C+ E  Y +CPHTA AL + Y      G     + TAS  KF  A  +AGL      +  L  L++K+     L   ED    L+  I
Sbjct:    1 MQYCSTRGEVHGWSFRDVLFSGYAPDGGMFMPEEIPILPPATLRSWSSLSYQQLVCEVCSLFIPEQLIPRHDLEALISS-ALSKFTVPDAVRMVKLKDSVSILELFHGETLAFKDLAMMCTTRFLEYFL---SKES----RHATILVGTSGDTGGSAI-SSACGLNGVDVVVVFPRGRITMVQEKQMTTHMEDNVHVFAADGSSDEIDVPLRKLFTDLDLVKSHG---LMSLNSGELVTNSA-------------------------------GCIVKRMGIPLRLVAVVNSNDILHRAVQNGDFSMAQSVKQTLAPAIDIQDPYNMERVFWLLSGEDGALVKDMMEQFQKTHRLTLPETLHKELCSV-LSSGSVSDEGIMEAMVRCWKENQYLVCPHTAVALCHHYRCPIPPGEKRCCIATASPAKFLSAVQKAGLSLDLPEA--LKSLETKETRCKDLERGEDWEAALRECI 428          
BLAST of Threonine synthase-like 2 vs. Ensembl Cavefish
Match: THNSL1 (threonine synthase like 1 [Source:HGNC Symbol;Acc:HGNC:26160])

HSP 1 Score: 134.035 bits (336), Expect = 5.259e-33
Identity = 120/440 (27.27%), Postives = 197/440 (44.77%), Query Frame = 3
Query:  120 FSEAILSGLCPDGGLLMP-EFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSK-FDKNIVEIKK--LKSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSL-------ESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNI------SCLNSINCGRIICQMALHIFIYLQLAEK----IGDTVVIAIPCGGMGNLISACLANEMGIPIELIVC-TNENDLGNKLIKTGIIE-KKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEA 1370
            FS+ ++ GL PDGGL +P +  PK   ++     + SY    +  L+  +   D     +  +  + + K F    V   K  L+  +V+ELFHGPT +FKD  L  +  L    +          M    +L+AT+GDTGS+ L       ES R   + + + V FP D ++  Q+LQMT     N  +I      D     I+ +  D        +      S  NSIN  R++ Q+  H   YL L +      GD + + IP G  GN +SA  A +MGIPI  I+C +N+N + +  I TG  + +      S S A+D     NL R ++  S  D    +     +E+ +   +   +++ +R  +   + + +D  L TI   +++  Y +  HTA A +     ++      +  TA   KF+ A ++A
Sbjct:  118 FSDVVVEGLAPDGGLYVPAKGFPKLEASEWIRLADASYSERALIILEKCIHPVDVSPLELRSMVDQAYGKNFASEFVAPVKHLLEDQYVLELFHGPTASFKDLALQLMPQLFAHCLPQ--------MCNYLILVATSGDTGSAVLSGFSRLKESDR---ERVGLLVFFPEDGVSHIQKLQMTGFKEGNARSISVLSDFDFCQRSIKRMFGDSSLVGHLAVEYAAVLSTANSINWARLLPQVVYHCSAYLDLLKSGTIGFGDQIDVCIPTGNFGNAMSAVYAKQMGIPIRKIICASNQNCVISDFISTGRYDLRGRKLIVSNSPAIDILKSSNLERFIYHASGGDGGLVRDLFQSLEKQQCFRVSENLLKTMRQEVHGGWCSEED-CLATIHKVYSKTGYIMDTHTAVAKAVADRVHDRTCPLVLCSTAHHGKFAPAVLKA 545          
BLAST of Threonine synthase-like 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008621.1 (pep scaffold:Pmarinus_7.0:GL477138:106992:108480:-1 gene:ENSPMAG00000007812.1 transcript:ENSPMAT00000008621.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 83.5741 bits (205), Expect = 2.337e-19
Identity = 44/125 (35.20%), Postives = 67/125 (53.60%), Query Frame = 3
Query:   75 SMKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFDKN-IVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLI 443
             MK++ST       SF EA+ SG   DGGL +PE +P+ +   L  W+ LSYP L+       +  ++   + +  + +   S+F  + +V  K+L+    V+ELFHGPT AFKD  +  LA  I
Sbjct:   12 QMKYVSTRGAAAGSSFREALFSGYAVDGGLFVPEEVPRVSADTLRQWRALSYPQLVQNICSLYISPDEIPVQELGALIERALSRFAHSEVVTFKRLRDGLNVLELFHGPTMAFKDLAMCCLAQFI 136          
BLAST of Threonine synthase-like 2 vs. Ensembl Yeast
Match: THR4 (Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway [Source:SGD;Acc:S000000649])

HSP 1 Score: 195.667 bits (496), Expect = 1.829e-56
Identity = 134/442 (30.32%), Postives = 220/442 (49.77%), Query Frame = 3
Query:  117 SFSEAILSGLCPDGGLLMPEFLPKF-TITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFDKNIVEIKKL--------KSCFVVELFHGPTKAFKDYPLFPLAYLIDKAI-KIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAE-KIGDTVVIAIPCGGMGNLISACLANEMGIPIE-LIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDH---------DTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDD--ETLKTI-STCFNEEHYCICPHTAT---ALSYFYHKNEVGNINAI-LGTASIEKFSEA 1358
            SF EAI+ GL  DGGL +P  +P+    T    W  LS+  L    ++  + + +     + D+ K ++S F  +  E+  L        ++  ++ELFHGPT AFKD  L  +  L +  + +  A   +G  K+  ++ AT+GDTGS+++   R   D + V++L+P  RI+P Q  QMTT   +N   +  +GT D   D+++ I  DKEF+   N+  +NSIN  RI+ QM  + + + Q    K    V   +P G  G++++   A +MG+PIE L + TNEND+ ++ +K+G+ E+ +    + S AMD  +  N  RLLW  +                 +   +++   K  +   I+E           +N++  ET+K I  +  N +HY + PHTA    A      K+   +I  I L TA   KF++A
Sbjct:   20 SFEEAIIQGLATDGGLFIPPTIPQVDQATLFNDWSKLSFQDLAFAIMRLYIAQEEIPDADLKDLIKRSYSTFRSD--EVTPLVQNVTGDKENLHILELFHGPTYAFKDVALQFVGNLFEYFLQRTNANLPEGEKKQITVVGATSGDTGSAAIYGLRGKKD-VSVFILYPTGRISPIQEEQMTTVPDENVQTLSVTGTFDNCQDIVKAIFGDKEFNSKHNVGAVNSINWARILAQMTYYFYSFFQATNGKDSKKVKFVVPSGNFGDILAGYFAKKMGLPIEKLAIATNENDILDRFLKSGLYERSDKVAATLSPAMDILISSNFERLLWYLAREYLANGDDLKAGEIVNNWFQELKTNGKFQVDKSIIEGASKDFTSERVSNEETSETIKKIYESSVNPKHYILDPHTAVGVCATERLIAKDNDKSIQYISLSTAHPAKFADA 458          
BLAST of Threonine synthase-like 2 vs. Ensembl Nematostella
Match: EDO37536 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFD7])

HSP 1 Score: 255.373 bits (651), Expect = 7.221e-79
Identity = 157/480 (32.71%), Postives = 251/480 (52.29%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETF--SKF-DKNIVEIKKL-KSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVT-IPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEV---GNINAILGTASIEKFSEAGIEAGLKDKFENSIF-LTQLQSKKEVFTVLNENEDPVTFLKNFIA 1490
            MKF ST    +  SF EAI +G   DGGLL+P+ +PK  I  L+ W  LSY  L  E L   V + + +   + +I  + +  SKF +KNI  + +L  +  ++ELFHG TKAFKD  L  +A  +D  +  K +          +L+ T+GDTGS+++ES R     + + VLFP       Q LQMTT   DN    +C G+ D+LD  ++    D+     +N+  +NSIN GR++ Q+  + + Y ++ +++G  V   +P G MGN+ +  +A  MG+P++L    N+N++ +++I  G     E+   +WS AMD  MPYN+ R+  I SD + +   S     E+   VT  P  +  K++SV+   + + +  TL T+   ++  HY +CPHTA  L     K +    G     + TAS +KF EA   AG+       I  L ++ +++E    + + ED    L++ IA
Sbjct:    1 MKFNSTRGKVKGLSFEEAIFTGYALDGGLLLPQAIPKLEIKILKEWATLSYKELCYEILSLFVSEEEVLRHDLKEIISKAYCESKFPNKNICNLTRLDNNLHILELFHGKTKAFKDNALVLVAAFLDFFLGRKKRHT-------TILVGTSGDTGSAAIESIRGLA-LIDIVVLFPKGYCNTIQELQMTTVIEDNVHVFECEGSSDDLDIPVKKCLTDEGLVTKYNLGSINSINIGRLLAQIVFYFYAYFKVCKEVGSIVKFVVPTGAMGNITAGYVAYNMGLPLKLHAAVNDNNIVHRMISQGDFSLAESITKTWSPAMDIQMPYNMERMWLIASDFNYDLVSSLTQTFEEHSAVTSFPETLTNKLKSVVSSDWVSRE-ATLDTMRRAWDSYHYLVCPHTAVGLRLALQKRDAEDDGTPVVCVATASPDKFPEAVKAAGIDVPPSPDILRLAEMPTRRE---FMRQGEDWEKILRDKIA 468          
BLAST of Threonine synthase-like 2 vs. Ensembl Medaka
Match: thnsl2 (threonine synthase like 2 [Source:NCBI gene;Acc:101172788])

HSP 1 Score: 233.802 bits (595), Expect = 4.427e-70
Identity = 133/443 (30.02%), Postives = 231/443 (52.14%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFD-KNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAE----KIGDTVV---IAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINA-ILGTASIEKFSEAGIEAGL 1376
            M++ ST       SF + + SG  PDGG+ MPE LP      L SW+ LSYP ++VE     +       + +  + +E  S F    +V + +LK    V+ELFHG T AFKD  +    + +   +K   KE++    +  +++ T+GDTG S+++S++     L V V++P  R+TP Q   MTT   DN       G+ D++D  +R +  D++      +  LNS+N  RI+ Q+A  ++ YL+L+E    + G  +    + +P GG GN+ +A +  +MG+P+ L+   N ND+ ++ +  G     +    + ++A+D   PYN+ R+ W+    D    K+ M + ++  + ++  +   ++ S ++ + A +D+  L+T+  C+ E  Y +CPHTA A+ + YH      +N   + TAS  KF  A  +AGL
Sbjct:    1 MQYCSTRGGVGGWSFRDVLFSGYAPDGGMFMPETLPVLDPDALRSWRGLSYPRVVVEVASLFIPPQLIPRDDLGVLVEEALSGFSVPEVVRVARLKDGLTVLELFHGETLAFKDLAMSCTVHFLSYFLK---KESR----RAVVVVGTSGDTGGSAIQSAKGLPG-LDVVVVYPRGRVTPVQEKHMTTCLEDNVHVFAADGSSDDIDQPLRRLFADQQLVLSHGLMSLNSVNWSRILIQLAHFVYAYLELSEVERAEPGAALPELEVVVPTGGAGNIAAAFIVKQMGVPLRLVAMVNANDIVHRTVTRGDFSMADKVTQTLATAIDIQDPYNMERVFWLLLGRDGASVKAMMEEFQRSHRHSLSEE-QRRLLSDVLSTGAVSDEGILETMRRCWEENLYLLCPHTAVAVWHHYHCPHSWGVNRCYVATASPAKFQAAVQKAGL 434          
BLAST of Threonine synthase-like 2 vs. Ensembl Medaka
Match: thnsl2 (threonine synthase like 2 [Source:NCBI gene;Acc:101172788])

HSP 1 Score: 194.126 bits (492), Expect = 2.964e-56
Identity = 107/360 (29.72%), Postives = 186/360 (51.67%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFD-KNIVEIKKLKSCF-VVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAE----KIGDTVV---IAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEK 1130
            M++ ST       SF + + SG  PDGG+ MPE LP      L SW+ LSYP ++VE     +       + +  + +E  S F    +V + +LK    V+ELFHG T AFKD  +    + +   +K   KE++    +  +++ T+GDTG S+++S++     L V V++P  R+TP Q   MTT   DN       G+ D++D  +R +  D++      +  LNS+N  RI+ Q+A  ++ YL+L+E    + G  +    + +P GG GN+ +A +  +MG+P+ L+   N ND+ ++ +  G     +    + ++A+D   PYN+ R+ W+    D    K+ M + ++  +
Sbjct:    1 MQYCSTRGGVGGWSFRDVLFSGYAPDGGMFMPETLPVLDPDALRSWRGLSYPRVVVEVASLFIPPQLIPRDDLGVLVEEALSGFSVPEVVRVARLKDGLTVLELFHGETLAFKDLAMSCTVHFLSYFLK---KESR----RAVVVVGTSGDTGGSAIQSAKGLPG-LDVVVVYPRGRVTPVQEKHMTTCLEDNVHVFAADGSSDDIDQPLRRLFADQQLVLSHGLMSLNSVNWSRILIQLAHFVYAYLELSEVERAEPGAALPELEVVVPTGGAGNIAAAFIVKQMGVPLRLVAMVNANDIVHRTVTRGDFSMADKVTQTLATAIDIQDPYNMERVFWLLLGRDGASVKAMMEEFQRSHR 352          
BLAST of Threonine synthase-like 2 vs. Ensembl Medaka
Match: THNSL1 (threonine synthase like 1 [Source:NCBI gene;Acc:101171618])

HSP 1 Score: 134.806 bits (338), Expect = 5.955e-33
Identity = 128/505 (25.35%), Postives = 222/505 (43.96%), Query Frame = 3
Query:   84 FISTNNDYEVHS-----FSEAILSGLCPDGGLLMPE-FLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFDKN-----IVEIKKL-KSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLES-SRFY---TDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELD----DVIRFINDDKEFSKLFN-----ISCLNSINCGRIICQMALHIFIYLQLAE----KIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVC-TNENDLGNKLIKTGIIE-KKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAIL-GTASIEKFSEAGIEA-GLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFIALHN 1499
            ++ST    E  S     FS+  + GL  DGGL +P    P+    + +   ++SYP   +  L+  +    P+    +D+    F  +  N     +  +K L +  FV+ELFHGPT +FKD  L  +  L    + +        M    +++AT+GDTGS+ L   SR          V V FP + ++  Q+LQM      NG A+   G   + D     + R   +      L       +S  NSIN  R++ Q+  H   YL L      + G+ V + IP G  GN +SA  A +MG+PI  ++C +N N +   LI TG  + ++     S S A+D     N+ R ++  SD D    +   ++++  +   +   ++ ++R   + +   ++DE L  +        Y +  HT  A +    + + G+   +L  TA   KF+ A  +A G+++  E+ +   QL+  +        + D +  L+     H+
Sbjct:  209 YVSTRRSGEERSKDKAYFSDVAVEGLAADGGLYVPRNGFPQVDAGEWKRLVSMSYPERALLLLEKCIH---PLDVSAADLRSMVFKAYGSNFSSEQVAPVKHLMEHQFVLELFHGPTGSFKDLALQLMPQLFAHCLPL--------MCNYLVVVATSGDTGSAVLSGFSRLSGVDAQRTGVLVFFPEEGVSEIQKLQMLGFGEGNGRAV---GVLSDFDFCQKSIKRMFGESGLVGHLAVEYGTVLSTANSINWARLLPQVVYHSSCYLDLCRDGVIRFGEPVDVCIPTGNFGNAMSAVYAKQMGVPIRKVICASNHNRVITDLINTGEYDLRRRRLLPSHSPAIDILKSSNVERFIFHASDGDGGLVEDLFTRLDTQKHFRVAAPLLGRMRQE-VQAGCCSEDECLSAVRRVHARTGYLMDTHTGVAKA-VADRLQDGSCPVVLCSTAHYGKFAPAVFKALGIQNPPEDPV--EQLRQLERTAPRPGGHRDMLRGLRGGSGAHS 695          
BLAST of Threonine synthase-like 2 vs. Planmine SMEST
Match: SMESG000081526.1 (SMESG000081526.1)

HSP 1 Score: 969.918 bits (2506), Expect = 0.000e+0
Identity = 471/474 (99.37%), Postives = 472/474 (99.58%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFDKNIVEIKKLKSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFIALHN 1499
            MKFISTNNDYEVHSFSEAIL+GLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLM EFLKFIVDKNDPVYEHISDISKETFSKFDKNIVEIKKLKSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKE FTVLNENEDPVTFLKNFIALHN
Sbjct:    1 MKFISTNNDYEVHSFSEAILTGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMAEFLKFIVDKNDPVYEHISDISKETFSKFDKNIVEIKKLKSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEDFTVLNENEDPVTFLKNFIALHN 474          
BLAST of Threonine synthase-like 2 vs. Planmine SMEST
Match: SMESG000081544.1 (SMESG000081544.1)

HSP 1 Score: 829.706 bits (2142), Expect = 0.000e+0
Identity = 390/474 (82.28%), Postives = 441/474 (93.04%), Query Frame = 3
Query:   78 MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLSYPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFDKNIVEIKKLKSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDTGSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGTPDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAEKIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTGIIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGEKVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATALSYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKKEVFTVLNENEDPVTFLKNFIALHN 1499
            MKFISTN  +EV SFS+AIL+GLCPDGGLLMPE+LPK TI QLESWKNLSYPYL+ EFLKFIVDKNDPV+E ISDISKETF+KF KN VEIKKLKSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKG+MKKQNLLIAT+GDTGSS+LE+SRFYTDTL VYVLFPNDRITPTQRLQMTT N++NGFAI C GT DE++D IR INDDKE+SKLFNISCLNSINCGRIICQMALH+FIYLQ+AEK+GDT+V+AIPCGG GNL+ ACL NEMG+PI+LI+CTNENDLG+KLIKTG++EK+ETSH+SWSSAMDT +PYN+ RL W++S HDTNFTKSTMSKI+QGEKVTIP KIMEKIRSVIIDSYA++ ++TL+T+S+CFNEEHYCICPHTATALSYFYHK E+ N+  +LGTASI KFSE GIEAG+ +KFENSIFLTQLQ+K+E +TV+NENEDPVTFLKNFIALHN
Sbjct:    1 MKFISTNGGFEVSSFSDAILTGLCPDGGLLMPEYLPKLTIVQLESWKNLSYPYLIAEFLKFIVDKNDPVFEDISDISKETFTKFCKNTVEIKKLKSCFVVELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGVMKKQNLLIATSGDTGSSALEASRFYTDTLRVYVLFPNDRITPTQRLQMTTVNSENGFAIGCPGTCDEIEDYIRSINDDKEYSKLFNISCLNSINCGRIICQMALHVFIYLQIAEKVGDTLVVAIPCGGWGNLLGACLVNEMGLPIKLILCTNENDLGDKLIKTGMVEKQETSHFSWSSAMDTILPYNIARLFWMYSGHDTNFTKSTMSKIDQGEKVTIPPKIMEKIRSVIIDSYASDCNDTLETMSSCFNEEHYCICPHTATALSYFYHKKEIDNVKVVLGTASISKFSEVGIEAGIIEKFENSIFLTQLQNKEEFYTVMNENEDPVTFLKNFIALHN 474          
The following BLAST results are available for this feature:
BLAST of Threonine synthase-like 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
THNSL23.354e-7832.02threonine synthase like 2 [Source:HGNC Symbol;Acc:... [more]
THNSL28.414e-6233.60threonine synthase like 2 [Source:HGNC Symbol;Acc:... [more]
THNSL13.681e-3828.57threonine synthase like 1 [Source:HGNC Symbol;Acc:... [more]
THNSL13.681e-3828.57threonine synthase like 1 [Source:HGNC Symbol;Acc:... [more]
THNSL21.734e-3534.63threonine synthase like 2 [Source:HGNC Symbol;Acc:... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Threonine synthase-like 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Threonine synthase-like 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
thnsl23.361e-7631.00threonine synthase-like 2 [Source:NCBI gene;Acc:64... [more]
thnsl21.114e-6030.35threonine synthase-like 2 [Source:NCBI gene;Acc:64... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
krtcap32.384e-7834.44keratinocyte associated protein 3 [Source:Xenbase;... [more]
slc49a47.111e-3727.64solute carrier family 49 member 4 [Source:Xenbase;... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Thnsl23.666e-7933.79threonine synthase-like 2 (bacterial) [Source:MGI ... [more]
Thnsl23.666e-7933.79threonine synthase-like 2 (bacterial) [Source:MGI ... [more]
Thnsl13.090e-3627.52threonine synthase-like 1 (bacterial) [Source:MGI ... [more]
Thnsl22.281e-1327.03threonine synthase-like 2 (bacterial) [Source:MGI ... [more]
Thnsl18.617e-827.16threonine synthase-like 1 (bacterial) [Source:MGI ... [more]
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BLAST of Threonine synthase-like 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5M7T9|THNS2_RAT2.406e-7832.50Threonine synthase-like 2 OS=Rattus norvegicus OX=... [more]
sp|Q80W22|THNS2_MOUSE2.564e-7833.79Threonine synthase-like 2 OS=Mus musculus OX=10090... [more]
sp|Q5XH07|THNS2_XENLA1.275e-7733.68Threonine synthase-like 2 OS=Xenopus laevis OX=835... [more]
sp|Q86YJ6|THNS2_HUMAN1.611e-7732.02Threonine synthase-like 2 OS=Homo sapiens OX=9606 ... [more]
sp|Q5RFE6|THNS2_PONAB3.009e-7731.46Threonine synthase-like 2 OS=Pongo abelii OX=9601 ... [more]
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BLAST of Threonine synthase-like 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3KBX22.305e-8233.54threonine synthase-like 2 isoform X1 OS=Lingula un... [more]
A0A099ZC603.561e-8233.33Threonine synthase-like 2 (Fragment) OS=Tinamus gu... [more]
K1PM145.236e-8232.35Threonine synthase-like 2 OS=Crassostrea gigas OX=... [more]
A0A1S3KD041.073e-8134.11threonine synthase-like 2 isoform X2 OS=Lingula un... [more]
A0A485PR692.314e-8133.19Threonine synthase-like 2-like OS=Lynx pardinus OX... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
thnsl25.391e-5529.32threonine synthase-like 2 [Source:NCBI gene;Acc:64... [more]
THNSL15.259e-3327.27threonine synthase like 1 [Source:HGNC Symbol;Acc:... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000008621.12.337e-1935.20pep scaffold:Pmarinus_7.0:GL477138:106992:108480:-... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
THR41.829e-5630.32Threonine synthase; conserved protein that catalyz... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO375367.221e-7932.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Threonine synthase-like 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
thnsl24.427e-7030.02threonine synthase like 2 [Source:NCBI gene;Acc:10... [more]
thnsl22.964e-5629.72threonine synthase like 2 [Source:NCBI gene;Acc:10... [more]
THNSL15.955e-3325.35threonine synthase like 1 [Source:NCBI gene;Acc:10... [more]
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BLAST of Threonine synthase-like 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000081526.10.000e+099.37SMESG000081526.1[more]
SMESG000081544.10.000e+082.28SMESG000081544.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003523 ID=SMED30003523|Name=Threonine synthase-like 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1653bp
CTATTTAAAAGCGACAAAATGATTATTCAATTACAAATTCTCCTGGATTA
CTTTTTAGTCTCATTGTTGAATTATCAATGAAATTTATAAGTACAAACAA
CGATTATGAAGTTCATTCATTTTCAGAAGCTATTCTTTCAGGGTTATGTC
CAGACGGTGGTTTATTGATGCCAGAATTTTTACCAAAGTTTACGATAACT
CAACTTGAAAGTTGGAAAAATTTAAGTTATCCTTATCTCATGGTAGAATT
TTTGAAATTTATTGTGGATAAAAATGATCCAGTTTATGAACATATTTCTG
ATATAAGCAAAGAAACATTTTCTAAATTTGATAAAAATATTGTGGAAATA
AAAAAATTAAAAAGTTGTTTTGTTGTCGAATTATTTCATGGACCAACGAA
AGCATTTAAGGATTACCCATTGTTTCCATTGGCTTATCTTATTGACAAAG
CTATTAAAATTAAAGCTAAAGAAAATAAAGGTATCATGAAAAAACAAAAT
CTGTTAATAGCAACGACTGGTGATACCGGCAGTAGTTCATTGGAATCATC
AAGATTTTACACGGATACTTTATGCGTCTATGTTTTATTTCCAAATGATA
GAATTACACCAACTCAAAGATTACAAATGACTACTGCAAATACTGATAAT
GGCTTTGCAATACAATGTTCTGGTACTCCGGATGAATTAGATGACGTTAT
TCGGTTTATTAATGATGATAAGGAATTTAGTAAATTATTTAACATTTCTT
GTCTCAATTCTATCAATTGTGGCCGAATTATATGCCAAATGGCGCTACAT
ATTTTTATTTACTTACAATTAGCGGAAAAAATCGGTGATACTGTAGTGAT
TGCCATACCATGTGGAGGAATGGGGAATTTAATAAGTGCTTGTTTAGCAA
ATGAAATGGGCATACCGATTGAACTTATTGTTTGTACAAACGAGAATGAT
TTAGGAAATAAACTTATTAAAACTGGAATTATTGAAAAGAAAGAAACTTC
TCATTATTCTTGGTCATCTGCAATGGATACTAATATGCCATATAATCTTG
TAAGACTGTTATGGATATTTTCTGATCACGATACAAATTTCACTAAATCA
ACAATGAGCAAAATCGAACAAGGAGAGAAAGTAACAATTCCACTTAAGAT
TATGGAAAAAATTCGTTCGGTAATAATCGATTCGTACGCTGCGAATGATG
ATGAAACTTTAAAAACAATATCTACTTGTTTCAATGAAGAGCATTACTGT
ATTTGTCCTCATACTGCAACAGCTCTTTCATACTTTTACCATAAAAATGA
AGTTGGTAATATCAATGCAATTCTAGGAACTGCAAGTATTGAGAAATTTA
GCGAAGCAGGAATTGAAGCTGGATTAAAAGATAAATTTGAAAATAGCATC
TTTTTAACTCAACTTCAAAGTAAGAAGGAAGTTTTTACTGTTTTGAATGA
AAATGAAGATCCAGTAACTTTTTTGAAAAATTTTATAGCTTTGCACAATT
GAATAACATTTTCCTAAGTTTATTGATTCGTAGAATTTTGTATTTGTCAT
TTGAATTAGACATTTGAATAAAATTTGATTTTTTATATTTGTAATGCACA
TTTATTTGGTCATTTTTTATTTAATATATTTTTGTATAAACTCATTAAAT
GCT
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protein sequence of SMED30003523-orf-1

>SMED30003523-orf-1 ID=SMED30003523-orf-1|Name=SMED30003523-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=475bp
MKFISTNNDYEVHSFSEAILSGLCPDGGLLMPEFLPKFTITQLESWKNLS
YPYLMVEFLKFIVDKNDPVYEHISDISKETFSKFDKNIVEIKKLKSCFVV
ELFHGPTKAFKDYPLFPLAYLIDKAIKIKAKENKGIMKKQNLLIATTGDT
GSSSLESSRFYTDTLCVYVLFPNDRITPTQRLQMTTANTDNGFAIQCSGT
PDELDDVIRFINDDKEFSKLFNISCLNSINCGRIICQMALHIFIYLQLAE
KIGDTVVIAIPCGGMGNLISACLANEMGIPIELIVCTNENDLGNKLIKTG
IIEKKETSHYSWSSAMDTNMPYNLVRLLWIFSDHDTNFTKSTMSKIEQGE
KVTIPLKIMEKIRSVIIDSYAANDDETLKTISTCFNEEHYCICPHTATAL
SYFYHKNEVGNINAILGTASIEKFSEAGIEAGLKDKFENSIFLTQLQSKK
EVFTVLNENEDPVTFLKNFIALHN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR037158Thr_synth_N_sf
IPR036052Trypto_synt_PLP_dependent
IPR029144Thr_synth_N
IPR001926PLP-dep
Vocabulary: biological process
TermDefinition
GO:0009071serine family amino acid catabolic process
GO:0016311dephosphorylation
GO:00463602-oxobutyrate biosynthetic process
Vocabulary: molecular function
TermDefinition
GO:0016829lyase activity
GO:0030170pyridoxal phosphate binding
GO:0070905serine binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029144Threonine synthase, N-terminalPFAMPF14821Thr_synth_Ncoord: 2..67
e-value: 1.9E-15
score: 56.8
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 101..403
e-value: 4.2E-9
score: 36.2
IPR037158Threonine synthase, N-terminal domain superfamilyGENE3DG3DSA:3.90.1380.10coord: 1..87
e-value: 1.4E-18
score: 68.8
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3DG3DSA:3.40.50.1100coord: 97..435
e-value: 5.5E-85
score: 287.2
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3DG3DSA:3.40.50.1100coord: 107..229
e-value: 5.5E-85
score: 287.2
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILYSSF53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 1..453
NoneNo IPR availablePANTHERPTHR42690FAMILY NOT NAMEDcoord: 1..471