SMED30003519

Overview
NameSMED30003519
Smed IDSMED30003519
Length (bp)2018
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003519 (SMED30003519) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003519 (SMED30003519) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003519

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30003519SMESG000018116.1 dd_Smed_v4_8136_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30003519SMESG000018116.1 dd_Smed_v4_8136_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003519SMESG000018116.1 dd_Smed_v4_8136_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30003519SMESG000018116.1 dd_Smed_v4_8136_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30003519SMESG000018116.1 dd_Smed_v4_8136_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30003519SMESG000018116.1 dd_Smed_v6_8136_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30003519SMESG000018116.1 dd_Smed_v4_8136_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003519 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.031617:3422..5439 +10090
Homology
[Close]
BLAST of SMED30003519 vs. Ensembl Human
Match: ZBED4 (zinc finger BED-type containing 4 [Source:HGNC Symbol;Acc:HGNC:20721])

HSP 1 Score: 45.4394 bits (106), Expect = 4.961e-8
Identity = 26/70 (37.14%), Postives = 41/70 (58.57%), Query Frame = -3 
Query:  940 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYIS 1149
            H LIQDVP++ ++  ++L+R IEQK+AI       N   LIS    D++ V+  + + L  F  A+R +S
Sbjct:  882 HHLIQDVPSKWSTSFHMLERLIEQKRAINEMSVECNFRELISC---DQWEVMQSVCRALKPFEAASREMS 948

HSP 2 Score: 32.7278 bits (73), Expect = 4.961e-8
Identity = 38/174 (21.84%), Postives = 73/174 (41.95%), Query Frame = -1 
Query: 1167 LPLIYQQAKTQLETFLSRA--IFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNC-------FLDISSFPEMHTHVNLMLKINEIINNW----NLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLK-RKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQK 1646
            +P +Y   K  + + L  A    + FT+    S  T  YL++ AH++  +S     C        LD+S     ++  ++  ++      W     LQ   V I   + A+I K  +      +QCF+H++ L ++  +K ++ +Q ++     +      S  A   L E Q+
Sbjct:  705 IPGMYDNVKQIIMSHLKEAESGVIHFTSGIWMSNQTREYLTLTAHWVSFESPARPRCDDHHCSALLDVSQVDCDYSGNSIQKQLECWWEAWVTSTGLQ---VGITVTDNASIGKTLNEGEHSSVQCFSHTVNLIVSEAIKSQRMVQNLLSLARKICERVHRSPKAKEKLAELQR 875
[Close]
BLAST of SMED30003519 vs. Ensembl Celegans
Match: C10A4.1 (pep chromosome:WBcel235:X:7415839:7418627:1 gene:WBGene00015662.1 transcript:C10A4.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C10A4.1)

HSP 1 Score: 49.6766 bits (117), Expect = 6.341e-6
Identity = 28/100 (28.00%), Postives = 50/100 (50.00%), Query Frame = -1 
Query: 1155 HTHVNLMLKINEIINNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRK-EIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1451
            HT  N+ L + E+I+ + ++ K V +  D  +N+  A D+L  E   C +H L LA N   K    + +++  +  +V   K ST  +   E  Q++L +
Sbjct:  221 HTAQNIALGLKELISEYKIEGKIVCMCRDGGSNVQNACDLLKCESFDCLSHKLHLAANDATKNMVGVGRVLLLYKKLVRKLKKSTVTAKQFESIQEVLEL 320
[Close]
BLAST of SMED30003519 vs. Ensembl Xenopus
Match: ZBED4 (zinc finger, BED-type containing 4 [Source:NCBI gene;Acc:100270676])

HSP 1 Score: 41.9726 bits (97), Expect = 4.431e-9
Identity = 26/70 (37.14%), Postives = 39/70 (55.71%), Query Frame = -3 
Query:  940 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYIS 1149
            H LIQDVP++ N+  ++L R IEQK+AI       +   LIS    D++ V+  +   L  F  A+R +S
Sbjct:  859 HHLIQDVPSKWNTSFHMLDRLIEQKRAIDEMSIECSFRELISC---DQWEVMQSVCHALKPFEAASREMS 925

HSP 2 Score: 39.6614 bits (91), Expect = 4.431e-9
Identity = 40/174 (22.99%), Postives = 76/174 (43.68%), Query Frame = -1 
Query: 1167 LPLIYQQAKTQLETFLSRA--IFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNC-------FLDISSFPEMHTHVNLMLKINEIINNW----NLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLK-RKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQK 1646
            +P +Y+  K  + + L  A    + FT     S  T  YL++ AH++  +S+F  +C        L +S     +  V++   +  +  +W     LQ   + I   +  +I+K  +      +QCF H++ L +N  +K ++ +Q ++     +      ST A   L E QK
Sbjct:  682 IPDMYENVKQIIVSHLKEAESGVIHFTAGIWMSSQTREYLTLTAHWVTFESSFRPHCENHHCSALLHVSQIDCDYNDVSVQKHLEYLWESWIASCGLQ---IGITVTDNHSIAKTLNESEHSSVQCFGHTVDLIVNEAIKSQRMVQNLLSIARKICERVHRSTKAKEKLAELQK 852
[Close]
BLAST of SMED30003519 vs. Ensembl Mouse
Match: Zbed4 (zinc finger, BED type containing 4 [Source:MGI Symbol;Acc:MGI:2682302])

HSP 1 Score: 43.8986 bits (102), Expect = 4.570e-7
Identity = 25/70 (35.71%), Postives = 41/70 (58.57%), Query Frame = -3 
Query:  940 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYIS 1149
            H LIQDVP++ ++  ++L+R IEQK+A+       N   LIS    D++ V+  +  +L  F  A+R +S
Sbjct:  881 HQLIQDVPSKWSTSFHMLERLIEQKRAVNEVSIECNFRELISC---DQWEVMQSVCHVLRPFDAASREMS 947

HSP 2 Score: 30.4166 bits (67), Expect = 4.570e-7
Identity = 38/174 (21.84%), Postives = 71/174 (40.80%), Query Frame = -1 
Query: 1167 LPLIYQQAKTQLETFLSRA--IFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNC-------FLDISSFPEMHTHVNLMLKINEIINNW----NLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLK-RKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQK 1646
            +P +Y   K  + + L  A    V FT+    S  T  YL++ AH++   S    +C        LD+S     ++  ++  ++      W     LQ   + I   +  +I K         +QCF+H++ L ++  +K ++ +Q ++     +      S  A   L E QK
Sbjct:  704 IPGMYDNVKQIIMSHLKEAESGVVHFTSGIWMSSQTREYLTLTAHWVTFASAVRPHCEDHHCSALLDVSQIDCDYSGNSIQKQLECWWEAWVTSIGLQ---IGITVTDNPSIGKMLSEGEHSSVQCFSHTVNLIVSEAIKSQRMVQNLLSIARKLCERVHRSPRAREKLAELQK 874
[Close]
BLAST of SMED30003519 vs. UniProt/SwissProt
Match: sp|O75132|ZBED4_HUMAN (Zinc finger BED domain-containing protein 4 OS=Homo sapiens OX=9606 GN=ZBED4 PE=1 SV=2)

HSP 1 Score: 45.4394 bits (106), Expect = 2.181e-7
Identity = 26/70 (37.14%), Postives = 41/70 (58.57%), Query Frame = -3 
Query:  940 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYIS 1149
            H LIQDVP++ ++  ++L+R IEQK+AI       N   LIS    D++ V+  + + L  F  A+R +S
Sbjct:  882 HHLIQDVPSKWSTSFHMLERLIEQKRAINEMSVECNFRELISC---DQWEVMQSVCRALKPFEAASREMS 948

HSP 2 Score: 32.7278 bits (73), Expect = 2.181e-7
Identity = 38/174 (21.84%), Postives = 73/174 (41.95%), Query Frame = -1 
Query: 1167 LPLIYQQAKTQLETFLSRA--IFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNC-------FLDISSFPEMHTHVNLMLKINEIINNW----NLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLK-RKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQK 1646
            +P +Y   K  + + L  A    + FT+    S  T  YL++ AH++  +S     C        LD+S     ++  ++  ++      W     LQ   V I   + A+I K  +      +QCF+H++ L ++  +K ++ +Q ++     +      S  A   L E Q+
Sbjct:  705 IPGMYDNVKQIIMSHLKEAESGVIHFTSGIWMSNQTREYLTLTAHWVSFESPARPRCDDHHCSALLDVSQVDCDYSGNSIQKQLECWWEAWVTSTGLQ---VGITVTDNASIGKTLNEGEHSSVQCFSHTVNLIVSEAIKSQRMVQNLLSLARKICERVHRSPKAKEKLAELQR 875
[Close]
BLAST of SMED30003519 vs. UniProt/SwissProt
Match: sp|Q80WQ9|ZBED4_MOUSE (Zinc finger BED domain-containing protein 4 OS=Mus musculus OX=10090 GN=Zbed4 PE=2 SV=1)

HSP 1 Score: 43.8986 bits (102), Expect = 2.775e-6
Identity = 25/70 (35.71%), Postives = 41/70 (58.57%), Query Frame = -3 
Query:  940 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYIS 1149
            H LIQDVP++ ++  ++L+R IEQK+A+       N   LIS    D++ V+  +  +L  F  A+R +S
Sbjct:  881 HQLIQDVPSKWSTSFHMLERLIEQKRAVNEVSIECNFRELISC---DQWEVMQSVCHVLRPFDAASREMS 947

HSP 2 Score: 30.4166 bits (67), Expect = 2.775e-6
Identity = 38/174 (21.84%), Postives = 71/174 (40.80%), Query Frame = -1 
Query: 1167 LPLIYQQAKTQLETFLSRA--IFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNC-------FLDISSFPEMHTHVNLMLKINEIINNW----NLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLK-RKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQK 1646
            +P +Y   K  + + L  A    V FT+    S  T  YL++ AH++   S    +C        LD+S     ++  ++  ++      W     LQ   + I   +  +I K         +QCF+H++ L ++  +K ++ +Q ++     +      S  A   L E QK
Sbjct:  704 IPGMYDNVKQIIMSHLKEAESGVVHFTSGIWMSSQTREYLTLTAHWVTFASAVRPHCEDHHCSALLDVSQIDCDYSGNSIQKQLECWWEAWVTSIGLQ---IGITVTDNPSIGKMLSEGEHSSVQCFSHTVNLIVSEAIKSQRMVQNLLSIARKLCERVHRSPRAREKLAELQK 874
[Close]
BLAST of SMED30003519 vs. TrEMBL
Match: W4YN88 (BED-type domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.354e-27
Identity = 55/174 (31.61%), Postives = 90/174 (51.72%), Query Frame = -1 
Query: 1155 RLPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPD---STFVYNC-----FLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIG-VVGHFKHSTTASNMLEEQQKLLNV 1649
            R+ L+Y+  K ++   L     V +TTD  TSI T  Y+++ AH I  D   + FV          + +  P+ HT   L  ++  ++ +W L+ K VA+ HDNAAN+ +  + +    + C AH+LQL +N  L      + + +    +VGHFKHS  AS  LE +Q+ + +
Sbjct:  120 RIRLLYEHKKEEIRESLVGVQHVSYTTDCWTSIGTRDYMTVTAHTIDEDWCRANFVLETKPVVGVGEEADQPQRHTTEALQNQLERVVEDWGLRRKSVAVVHDNAANVRRIGEGIDAADVGCAAHTLQLCVNTALDSAPANRTLSAAASRLVGHFKHSVVASKALEAEQRQMGI 293

HSP 2 Score: 41.2022 bits (95), Expect = 3.354e-27
Identity = 27/85 (31.76%), Postives = 44/85 (51.76%), Query Frame = -3 
Query:  907 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLIS----KLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSIL 1149
            H L+Q +  R NS   + KR  EQ+ AI   LS +   NL      +L D+++ ++D +I  L  F  AT  +S ++  T S ++
Sbjct:  296 HKLVQSMKVRWNSTFDMFKRLKEQRWAICRVLSDRRHTNLGDARTLELKDEQWRLLDGLISALEPFKVATTVLSGEEQTTLSVVV 380

HSP 3 Score: 37.3502 bits (85), Expect = 3.354e-27
Identity = 28/112 (25.00%), Postives = 49/112 (43.75%), Query Frame = -3 
Query: 1651 WDIFNEIYFKSAVCKLCNKNVSTNGATNN-MLQHVRLFHTXXXXXXXXXXXXXXKVEYY------------LYALICENRLPISFL-GGKCIAYTEPLNPIFKIPNRWTFSS 1944
            W +FN+I   S  C++C+K  + +G++   ML H+   H+     + ++      ++              +  LI    LPIS +  G   A  + L P +  P+R T SS
Sbjct:    8 WKVFNKISDNSVQCRICDKKYAYSGSSTGVMLSHIAKSHSTALDKDKSQPTMSDFIKAPPTPMRRETITDKIVELIARESLPISLVEAGAFKALLKCLEPGYVTPSRKTVSS 119
[Close]
BLAST of SMED30003519 vs. TrEMBL
Match: H3A927 (Dimer_Tnp_hAT domain-containing protein OS=Latimeria chalumnae OX=7897 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 8.246e-25
Identity = 56/162 (34.57%), Postives = 87/162 (53.70%), Query Frame = -1 
Query: 1155 YQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VAIEHDNAANI--SKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1634
            Y+     L   L  AI V FTTD  T++ T++Y+++  H+I  ++  + +  L   S  E HT  NL  K+N I+  W L    +A  HDNA+NI  +     +++E + CF+H+LQLAIN       + + + +   +V HF HST A+  LE +Q  L V
Sbjct:   89 YEDCVKSLWEKLEHAITVAFTTDCWTALTTESYMTVTCHYI--ENWELQSAVLQTESMAEQHTAENLAKKLNTIVEKWGLAGCVLACVHDNASNIVLANTPRYVTWESVNCFSHTLQLAINDGFSSSSVDRAIAAASRLVAHFHHSTVAAKALERKQTQLQV 248

HSP 2 Score: 45.4394 bits (106), Expect = 8.246e-25
Identity = 30/80 (37.50%), Postives = 46/80 (57.50%), Query Frame = -3 
Query:  910 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSI 1149
            H LIQ   TR NSV  + KR  EQ+ AI A LS  + +   S L D+++ +I+ I+ LL+    AT  +S ++  + S+I
Sbjct:  251 HQLIQSCKTRWNSVYDMFKRLSEQRWAITAVLS-DSSVTKQSDLQDEQWQLIEDILSLLSTLKCATTAMSAEQSVSISNI 329
[Close]
BLAST of SMED30003519 vs. TrEMBL
Match: A0A444UVN6 (Zinc finger BED domain-containing protein 1 OS=Acipenser ruthenus OX=7906 GN=EOD39_20358 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 6.037e-24
Identity = 54/152 (35.53%), Postives = 86/152 (56.58%), Query Frame = -1 
Query: 1167 LSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFP--EMHTHVNLMLKINEIINNWNLQNK*VA-IEHDNAANI----SKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQK 1601
            L+ A  +  TTD  TSI T+AYLS+ AHFI P  T      ++++S P  E HT  N++  + E++  + +    VA +  DN +N+    +K +++  +  + C  HSLQL IN+ LK   I++ +G+   VV HFK S  A+  L+ +QK
Sbjct:  118 LATASSIALTTDIWTSIATEAYLSVTAHFITPAWTMKT---INLTSKPLTERHTGGNIVAWVEEVLKKFEISPAQVAALVRDNGSNVVSTANKLSELHGWLSVSCAGHSLQLTINNALKNITIERAIGAARSVVEHFKKSELATTALKRKQK 266

HSP 2 Score: 57.3806 bits (137), Expect = 6.037e-24
Identity = 31/84 (36.90%), Postives = 53/84 (63.10%), Query Frame = -3 
Query:  910 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLS--TQNKINLISKLN--DDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSI 1149
            HTL+QDVPTR NS L+++KR +EQ+  I A LS  T      +  L+   D++++++ ++ +L  F  AT Y+S + Y + S++
Sbjct:  273 HTLLQDVPTRWNSSLHMIKRLLEQRWPIVAVLSDPTVKSARAVRHLDLSQDQWSLLNELVSVLQPFETATAYLSGETYVSLSAV 356
[Close]
BLAST of SMED30003519 vs. TrEMBL
Match: A0A3N0Y7T9 (Zinc finger BED domain-containing protein 1 OS=Anabarilius grahami OX=495550 GN=DPX16_18204 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.749e-23
Identity = 50/162 (30.86%), Postives = 91/162 (56.17%), Query Frame = -1 
Query: 1161 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLL 1646
            LP +Y++ K ++   + +A  V  T+D  TS+ T++Y++I +H+I  D   V +  L   +  E HT  ++   + +++  W L +K V +  DNAAN+  A +I  F  ++CFAH+L LA    LK   + +++G    +  +F  ST A ++ EE Q+++
Sbjct:  154 LPALYKETKAKVMESMCKARRVAITSDAWTSVATESYVTITSHYISEDWKIVSHV-LQTRAVYESHTGAHMARLLLDVVEEWQLTDKSVVLVTDNAANMISAAEIGKFPHVKCFAHTLNLAAQRALKLPAVSRLLGRVRRISAYFHRSTKAKHLFEENQRVV 314

HSP 2 Score: 36.1946 bits (82), Expect = 2.749e-23
Identity = 27/86 (31.40%), Postives = 44/86 (51.16%), Query Frame = -3 
Query:  916 LIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDL----------IIKLLTLF*EATRYISRDKYATGS 1143
            LI DV TR NS   +++RF++ + A++ATL       L   LN DE +++ L          ++K L    +AT ++S +   T S
Sbjct:  323 LITDVATRWNSAHDMMERFLQLQAAVHATL-------LSPALNVDESDIVTLSRADLANVEEVVKTLKPVKDATVFMSEESSPTVS 401

HSP 3 Score: 26.5646 bits (57), Expect = 2.749e-23
Identity = 23/110 (20.91%), Postives = 41/110 (37.27%), Query Frame = -3 
Query: 1648 AVCKLCNKNVSTNGATNNMLQHVRLFHTXXXXXXXXXX---XXXXKVEYYLYALICEN--------RLPISFLGGKCIAYT-----------EPLNPIFKIPNRWTFSST 1911
            AVCK+C+  +   G T N   HV  +H          +    +  +++  L + +  N        R    F+      Y+           + + P +K+P R TFS +
Sbjct:   43 AVCKICHTKIKHVGNTTNFTNHVDRWHPELASTITTTQKVDTSQPRIDESLVSTLPHNSERAKRITRSVACFIAKDLRPYSVVENAGFRHMLKTIEPRYKLPTRATFSDS 152
[Close]
BLAST of SMED30003519 vs. TrEMBL
Match: A0A1X7VJT8 (DUF659 domain-containing protein OS=Amphimedon queenslandica OX=400682 GN=105316672 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 3.308e-23
Identity = 57/165 (34.55%), Postives = 88/165 (53.33%), Query Frame = -1 
Query: 1158 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTF-VYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VA-IEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLN 1646
            LP +++  K ++   LS       TTD  TS    AY  +  HFI  D+ F +++  L+   FPE HT +N+  ++  I+  WNL  + VA I  DN  NI+ A   L +  I CF+H+LQL +   LK  ++ K V     +  HF HS+ +S +L+E+Q+ L 
Sbjct:   28 LPQLFEGEKMKIMHCLSDVKSFSLTTDIWTSRANHAYTGVTIHFI--DNDFKLHHFLLETKEFPESHTSLNIANELTSILKEWNLGAENVAAITTDNGRNITGAIRDLDWINIPCFSHTLQLGVQKVLKLPQVAKAVARCKKIATHFHHSSKSSYILKEKQRSLK 190

HSP 2 Score: 49.2914 bits (116), Expect = 3.308e-23
Identity = 27/85 (31.76%), Postives = 48/85 (56.47%), Query Frame = -3 
Query:  910 IKCSVHTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSI 1164
            +KC  H+++QDV T  NS+ Y++ R IEQ+Q I   L    K++L+    D E+  ++  + L+    + T  I  +K+ T S++
Sbjct:  189 LKCKEHSIVQDVATCWNSLYYMVSRIIEQQQPICTILLEIQKLDLMP--TDHEFKTMEEFVSLMKSLVDVTEAIGAEKWITISTL 271
[Close]
BLAST of SMED30003519 vs. Ensembl Cavefish
Match: ENSAMXT00000030725.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000043.1:648734:652625:-1 gene:ENSAMXG00000030453.1 transcript:ENSAMXT00000030725.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 93.5893 bits (231), Expect = 1.446e-25
Identity = 57/165 (34.55%), Postives = 89/165 (53.94%), Query Frame = -1 
Query: 1155 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTF-VYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1646
            +P +Y+  K  + T L     V  T D+  S   D+YL+I AH+I  D+ + + +  L        HT  NL   ++E I  W L NK  AI  DNAAN+++A ++ +   + CFAH+L LA    LK   + +++G    +   F  STTAS+ L+E+QK+L +
Sbjct:  170 IPNLYRDVKHGVATSLKSTERVALTCDSWVSRAMDSYLTITAHYI--DNEWRLMSHVLQTREIETSHTASNLAELLSEAIQEWELANKDPAIVTDNAANMTRAVELTNLLHVGCFAHTLNLASQAALKTPPVARLLGRVRRIASFFHRSTTASHQLKEKQKILGL 332

HSP 2 Score: 43.1282 bits (100), Expect = 1.446e-25
Identity = 32/91 (35.16%), Postives = 52/91 (57.14%), Query Frame = -3 
Query:  910 EIIKCSVHTLIQDVPTR*NSVLYILKRFIEQKQAIYATLST----QNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSI 1170
            +I+    H L+ DV TR NS L +L+RF+EQ+ AI A L +    +N+ +L + L++ +  V + ++K L     AT  +S +K  T S I
Sbjct:  328 KILGLPAHKLVIDVTTRWNSALEMLQRFLEQQPAISAALLSPEVRRNESDLCT-LSELDITVAEDVVKALGPLKAATLVMSEEKSPTLSVI 417
[Close]
BLAST of SMED30003519 vs. Ensembl Cavefish
Match: ENSAMXT00000057362.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001541.1:173309:174871:-1 gene:ENSAMXG00000043106.1 transcript:ENSAMXT00000057362.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.4925 bits (197), Expect = 9.172e-23
Identity = 51/165 (30.91%), Postives = 83/165 (50.30%), Query Frame = -1 
Query: 1155 IYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQ--NK*VAIEHDNAANISKATDILSF--ERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1637
            +Y++    +   LS A  +  TTD  TS  T++YL++ AH+I P      +C L      E HT   L  K+ E++  W L+  N  + +  DNA NI  A  +      +I CFAH++ LA    +   +I +++     VV  F  STTA+ +L+ +Q +L +
Sbjct:  178 LYEETSVDIVKELSEACQIALTTDGWTSRSTESYLTVTAHYITPQWEL-RSCVLQTRPIYESHTSKILSEKLREVVEEWKLERDNGTIPVTTDNANNIVNAVGLTEGLGPQISCFAHTVNLAAKSAISTAQISRLLAKVRKVVTFFHRSTTAAFILKTKQDMLTL 341

HSP 2 Score: 46.595 bits (109), Expect = 9.172e-23
Identity = 29/84 (34.52%), Postives = 47/84 (55.95%), Query Frame = -3 
Query:  907 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQN---KINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSIL 1149
            H LI DVPTR NS   +L+R++EQ+ A+Y+ ++ +N   K   IS L D E  + + +I +L      T  +S +   + S I+
Sbjct:  344 HKLIHDVPTRWNSTYDMLERYVEQQAALYSAVTDKNMKKKTKDISLLTDSETKLTEGLIDVLKPLKNVTTLMSTETSPSVSMIM 427
[Close]
BLAST of SMED30003519 vs. Ensembl Cavefish
Match: ENSAMXT00000045953.1 (zinc finger BED domain-containing protein 1-like [Source:NCBI gene;Acc:111189856])

HSP 1 Score: 79.7221 bits (195), Expect = 1.095e-22
Identity = 53/167 (31.74%), Postives = 86/167 (51.50%), Query Frame = -1 
Query: 1155 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNL--QNK*VAIEHDNAANISKATDILSFER-IQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1646
            LP +Y+  K  +   LS A  +  TTD  TS  T++Y+++ AH++  +   + +  L      E HT  NL   + E +  W L  QN  + +  DNA NI  A++       I CFAH++ LA    L   +I +++G    VV  F  STTA+ +L+ +Q++L +
Sbjct:  155 LPSMYEDTKENVLRGLSNAELIAITTDGWTSRATESYITVTAHYVN-NEWEIESPVLQTRPIYESHTSDNLAEVLQEAVLEWKLDRQNTTIPVTTDNAKNIVNASNAAGLSPHIGCFAHTVNLASQKGLGVNQISRLLGKVRRVVTFFHKSTTAAAVLKAKQEMLQL 320

HSP 2 Score: 47.3654 bits (111), Expect = 1.095e-22
Identity = 31/92 (33.70%), Postives = 55/92 (59.78%), Query Frame = -3 
Query:  907 EIIKCSVHTLIQDVPTR*NSVLYILKRFIEQKQAIYATLST----QNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSIL 1170
            E+++   H LIQDV TR NS   +L R++EQ+ A+Y+ L+     +N  +LI+ L+D +  V++ ++++L      T  +S ++  T S IL
Sbjct:  316 EMLQLPPHKLIQDVATRWNSSYDMLTRYLEQQAAVYSALTEKDVKKNAKDLIT-LSDQDVTVLEDVVEVLKPMKTITTLLSSEQQPTVSMIL 406
[Close]
BLAST of SMED30003519 vs. Ensembl Cavefish
Match: ENSAMXT00000046756.1 (pep primary_assembly:Astyanax_mexicanus-2.0:22:9736382:9743382:-1 gene:ENSAMXG00000040138.1 transcript:ENSAMXT00000046756.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 89.3521 bits (220), Expect = 1.694e-19
Identity = 53/164 (32.32%), Postives = 87/164 (53.05%), Query Frame = -1 
Query: 1155 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1646
            +P +YQ+ K ++   L++   V  T D  TS  T ++++  AH+I  D+  + +  L      E HT VN+    + + + W L    + I  DNAAN+  AT I +   I CFAH+L LA    LK   + +++G    + G F  ST A++ L+E++KLL +
Sbjct:  128 VPQLYQETKRKVANTLTKTTRVALTCDAWTSRATQSFVTFTAHYIT-DTWLLESRVLQTRVMHESHTAVNVNAMFHSVADEWELTVTDLVIVTDNAANMLAATQIDNLTHITCFAHTLNLAAQRALKLPTVSRLLGRIRRITGFFHRSTVANHELQEKKKLLQL 290

HSP 2 Score: 26.9498 bits (58), Expect = 1.694e-19
Identity = 21/100 (21.00%), Postives = 39/100 (39.00%), Query Frame = -3 
Query: 1633 EIYFKSAVCKLCNKNVSTNGATNNMLQHVRLFHTXXXXXXXXXXXXXXKVEYYLYALICENRLPISFLGGKCIAYT-EPLNPIFKIPNRWTFSSTTATHL 1929
            E+     VCK+C   +   G T N   H+   H   +  E ++     +    +   I ++  P S +  +   +  +   P + IP+R  F+ T    L
Sbjct:   32 ELDMSFIVCKVCKMKIKNFGNTTNARAHITRHHPELKDAEQSQPPAADQRTLSIACFIAKDLRPYSVVENEGFTHMLQTAEPRYAIPSRKFFTETAVPQL 131
[Close]
BLAST of SMED30003519 vs. Ensembl Cavefish
Match: ENSAMXT00000047448.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002238.1:20880:24927:1 gene:ENSAMXG00000041080.1 transcript:ENSAMXT00000047448.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 80.4925 bits (197), Expect = 1.900e-19
Identity = 55/174 (31.61%), Postives = 91/174 (52.30%), Query Frame = -1 
Query: 1152 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQN-K*VAIEHDNAANISKATDILSFERIQ--------CFAHSLQLAINHCLKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNVL 1646
            L L Y   + Q+   +  +  +  TTD  TS+  +AY+S+  HFI  +     N  L   +    HT V++   + E+  ++ + N K VA+ HDNA+N+  +T+ L  E  +        C  H+LQL IN  LK+  I++ + +   +VGHFK S  A+  L+++QK  NV+
Sbjct:   78 LSLKYSDLQDQVSALIENSSALSLTTDIWTSLNMEAYMSVTCHFINQEWELC-NFVLATKAMEVSHTGVHIAQLLGEVAESFAIPNYKRVAVVHDNASNMKLSTETLKKEPEKWGNVQGVFCSGHTLQLCINSALKQDRIRRTISAARNLVGHFKKSAKATIALKDKQKQQNVV 250

HSP 2 Score: 35.4242 bits (80), Expect = 1.900e-19
Identity = 22/84 (26.19%), Postives = 46/84 (54.76%), Query Frame = -3 
Query:  907 HTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQN---KINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSIL 1149
            H+L QD+ TR NS   +L R +EQ+  + A LS  +   K +    L+ +++ +   ++++L      T  +S++++ + SS +
Sbjct:  252 HSLTQDIATRWNSTCEMLDRLLEQRWPVTAVLSDPSITKKGDRTLDLSTEQWKLAQDLVEVLRPLITLTEVLSQEEHVSLSSAV 335
[Close]
BLAST of SMED30003519 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001841.1 (pep scaffold:Pmarinus_7.0:GL477152:41279:42850:-1 gene:ENSPMAG00000001672.1 transcript:ENSPMAT00000001841.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 6.097e-14
Identity = 43/162 (26.54%), Postives = 71/162 (43.83%), Query Frame = -1 
Query: 1167 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTD-AYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCL-KRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQK 1646
            +P  Y +    +   L  A ++ FTTD  ++     + LS   HFI  D+ +     L ++   + H    L  K+ E +  W +  K      DNAAN+  A  +     I C +H+LQL +   L  +  ++ ++     +V H KHS  A   L E Q+
Sbjct:   75 MPATYDKVVKHVTNLLQEATWISFTTDGWSNPTKSCSLLSFTGHFIH-DAVW-RKVILSVTVLEQDHDGKYLASKLCEAMTKWEIAEKIHVGVRDNAANMKLAMRLAGVADIGCMSHTLQLVLRDALFTQTSVEVVIKKARKIVTHLKHSEQACRHLVEHQQ 234

HSP 2 Score: 37.7354 bits (86), Expect = 6.097e-14
Identity = 24/79 (30.38%), Postives = 43/79 (54.43%), Query Frame = -3 
Query:  934 EIIKCSVHTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRD 1170
            + +K   H+L+QDV TR N +  +L+R +EQ++A+      ++ I+  S     E+ +   ++K+L  F  AT  I  D
Sbjct:  234 QTVKTPEHSLLQDVETRWNGMYLMLERLVEQRKAVNLYSVQRSGIDSFSLA---EWELAARVVKILKPFYTATLEICVD 309
[Close]
BLAST of SMED30003519 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008230.1 (pep scaffold:Pmarinus_7.0:GL479508:145707:147445:-1 gene:ENSPMAG00000007442.1 transcript:ENSPMAT00000008230.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 43.8986 bits (102), Expect = 6.447e-10
Identity = 26/85 (30.59%), Postives = 39/85 (45.88%), Query Frame = -1 
Query: 1158 NNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCL-KRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLN 1409
              W +  K      DNAAN+  A  +     I C +H+LQL +   L  +  ++ +V     +V HFKHS  A   L E Q+ + 
Sbjct:  196 TKWEIAEKINVRVRDNAANMKMAMRLAGITDIGCMSHTLQLVLRDALFTQTSVEAVVKKARKIVTHFKHSEQACRHLVEHQQTIK 280

HSP 2 Score: 37.3502 bits (85), Expect = 6.447e-10
Identity = 16/35 (45.71%), Postives = 25/35 (71.43%), Query Frame = -3 
Query: 1066 EIIKCSVHTLIQDVPTR*NSVLYILKRFIEQKQAI 1170
            + IK   H+L+QDV TR NS   +L+R +EQ++A+
Sbjct:  277 QTIKSPEHSLLQDVDTRWNSAYLMLERLVEQRKAV 311
[Close]
BLAST of SMED30003519 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008876.1 (pep scaffold:Pmarinus_7.0:GL476791:39849:44790:1 gene:ENSPMAG00000008036.1 transcript:ENSPMAT00000008876.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.3066 bits (142), Expect = 2.033e-9
Identity = 48/168 (28.57%), Postives = 85/168 (50.60%), Query Frame = -1 
Query: 1152 YQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQN-K*VAIEHDNAANISKATDIL----SFERIQCFAHSLQLAI-NHCL-KRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNVL 1634
            Y + K Q    L +A  +  + D   S   DAY+S++ HFI  D     N  L+ ++  E HT + +   I + +  + ++  K V++ HD   N+S A  +L    S++ +QC AHSLQL + +H + K   + + V     +V +F+ S  A + L  ++K  N++
Sbjct:  151 YNEVKQQCIAELKKASGLSLSCDVWNSAAVDAYMSVSVHFIDEDWQL-RNFLLESTNVLESHTPIVIRQWIEDALQRFEVEPAKIVSMVHDTGENMSAAARLLEEAYSWKPVQCAAHSLQLIVKDHGISKLPALDRAVDVARQLVEYFRKSEFALSQLSVREKEYNII 317
[Close]
BLAST of SMED30003519 vs. Ensembl Medaka
Match: ENSORLT00000035819.1 (pep primary_assembly:ASM223467v1:2:6014183:6016710:-1 gene:ENSORLG00000024444.1 transcript:ENSORLT00000035819.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 70.8626 bits (172), Expect = 6.178e-21
Identity = 52/161 (32.30%), Postives = 80/161 (49.69%), Query Frame = -1 
Query: 1158 QLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFP--EMHTHVNLMLKINEIINNWNLQNK*V-AIEHDNAANISKATDILS----FERIQCFAHSLQLAINHCLK-RKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLN 1616
            +L+  L +   V  TTD  TS+ T+AYL +  HF+  D        L +++ P  E HT  N+   + E    + +  + V A+ HDN AN+  A  IL+    +  ++C  H+L L +   LK  K I   VGS   +V HFK S  A   L+E+Q+ + 
Sbjct:  151 KLKAVLKQTECVALTTDIWTSVATEAYLGVTCHFLGEDWEMKS---LSLTTMPLEERHTAANIADWLEETTAQFEIPREKVKAVVHDNRANVVAAARILTERHGWASVRCAGHTLNLVVQSTLKNNKTIASCVGSARCLVEHFKKSELACTKLKEKQQQMG 308

HSP 2 Score: 50.447 bits (119), Expect = 6.178e-21
Identity = 29/86 (33.72%), Postives = 50/86 (58.14%), Query Frame = -3 
Query:  910 VHTLIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISK-----LNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSI 1152
            +H LIQDV TR NS  ++L R +EQ+  + A LS    +N   K     L  +++++ + + ++L  F  AT ++S +KY T S++
Sbjct:  311 LHMLIQDVSTRWNSTYHMLSRLLEQRWPVTAVLS-DPAVNPRGKHHYLDLKPEQWHISEELTQILGPFEGATEFLSGEKYVTLSAL 395
[Close]
BLAST of SMED30003519 vs. Ensembl Medaka
Match: ENSORLT00000046033.1 (pep primary_assembly:ASM223467v1:18:700339:706184:1 gene:ENSORLG00000022856.1 transcript:ENSORLT00000046033.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.6918 bits (143), Expect = 7.143e-9
Identity = 44/177 (24.86%), Postives = 73/177 (41.24%), Query Frame = -1 
Query: 1161 QSLKFQIDGHFLLRLPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIG-VVGHFKHSTTASNMLEEQQKLL 1688
            Q+LK  +D          YQ+ K   +  L  A  V  T D  TS   D  L++  H+++ +S       L +  FP+ H   ++      ++  W L+ K   +  D    +  A D L+   ++C AH+L   +   L +    K +    G VV  F+ S  A   L  +Q+ L
Sbjct:   97 QALKKMVDDR--------YQEEKADAQAQLREAEAVSLTVDLWTS--ADTRLAVACHYVLENSKLA-TVLLGVLPFPQNHGPTDVADAQRTLMAEWGLEGKVKCLVSDTTPAMVAAADDLALPHVECIAHALNGVVRRALDKTAGLKGLRRRAGEVVALFRSSAAAKEKLRSKQQKL 262
[Close]
BLAST of SMED30003519 vs. Ensembl Medaka
Match: ENSORLT00000037653.1 (pep primary_assembly:ASM223467v1:21:546436:551984:-1 gene:ENSORLG00000024821.1 transcript:ENSORLT00000037653.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.7582 bits (125), Expect = 9.843e-7
Identity = 40/151 (26.49%), Postives = 65/151 (43.05%), Query Frame = -1 
Query: 1203 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNL-QNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLK--RKEIQKIVGSFIGVVGHFKHS 1646
            +P  Y   K  +   L    +   T D  +S+    Y+S+  H++  +      C L+    PE HT  NL   +      W+L + K + I  DN ANI  A   L +  + CF H+L LA+ + +K  +    + +G    +V  F  S
Sbjct:  172 IPEKYLSVKDGVIQELKYVDYFSVTADMWSSVNNMPYMSLTIHYLSANWELKSKC-LETVFTPESHTADNLAEALRSSFEEWSLDERKLICITTDNGANIVAAVRKLGWTWLNCFGHNLHLAVTNSMKTEKDRTARAMGVCRNLVTAFSQS 321
[Close]
BLAST of SMED30003519 vs. Planmine SMEST
Match: SMESG000034450.1 (SMESG000034450.1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.980e-14
Identity = 42/167 (25.15%), Postives = 82/167 (49.10%), Query Frame = -1 
Query: 1155 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSI-LTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*V-AIEHDNAANISKATDILSFERIQCFAHSLQLAINHC-LKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1646
            +P+++++ + ++   +S    V FT+D  +   ++ A+LS+ AH+                   E HT   L   + +I+ ++ ++   V  +  D+ AN+ K + ++    I CFAH+LQL IN   + ++ ++ I+     +VG F+HS  A   L + Q  L V
Sbjct:  154 IPVMHEKIQNKVREMVSSQPSVSFTSDAWSDPSISVAFLSLTAHW-------------------ESHTGDYLGGMLLKILGDYTIEKSAVHVLLRDSGANMIKESRVVDIHSISCFAHTLQLVINDSIMSQRSVKNILAISRSIVGKFRHSVLAQQYLSDLQGSLGV 301

HSP 2 Score: 40.817 bits (94), Expect = 2.980e-14
Identity = 24/68 (35.29%), Postives = 43/68 (63.24%), Query Frame = -3 
Query:  940 LIQDVPTR*NSVLYILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYIS 1143
            L+QDV TR N+ L +L+  +EQKQ + A  +T++++     L+  ++ +I  II  L+ F +AT+ +S
Sbjct:  306 LVQDVQTRWNATLAMLRSLVEQKQVLGA-YATEHELK---NLDAHQWTLISHIIDTLSPFEDATKKVS 369
[Close]
BLAST of SMED30003519 vs. Planmine SMEST
Match: SMESG000045018.1 (SMESG000045018.1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.166e-10
Identity = 46/167 (27.54%), Postives = 85/167 (50.90%), Query Frame = -1 
Query: 1155 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSI-LTDAYLSINAHFIMPDSTFVYNCFLDISSFPEMHTHVNLMLKINEIINNWNLQNK*V-AIEHDNAANISKATDILSFERIQCFAHSLQLAINHC-LKRKEIQKIVGSFIGVVGHFKHSTTASNMLEEQQKLLNV 1646
            +P ++++ + ++   +S    V FT+D  +   ++ A LS+ AH++  D        L  S   E HT   L   + +I+ ++ ++   V  +  D+ AN+ KA+ +   + I CFAH+LQL IN   + ++ ++ I+     +VG F+HS  A   L + Q  L V
Sbjct:  194 IPAMHEKIQNKVREMVSSQPSVSFTSDAWSDPSISVALLSLTAHWVTEDFQR-KQAILAASPIEESHTGDYLGGMLLKILGDYTIEKSAVHVLLRDSGANMIKASRVADIDSISCFAHTLQLVINDSIMSQRSVKDILAISRSIVGKFRHSVLAQQHLSDLQTSLGV 359
[Close]
BLAST of SMED30003519 vs. Planmine SMEST
Match: SMESG000026828.1 (SMESG000026828.1)

HSP 1 Score: 57.3806 bits (137), Expect = 8.833e-10
Identity = 32/78 (41.03%), Postives = 46/78 (58.97%), Query Frame = -1 
Query: 1416 LPLIYQQAKTQLETFLSRAIFVRFTTDT*TSILTDAYLSINAHFIMPDSTFVYNCF-LDISSFPEMHTHVNLMLKINE 1646
            LP +Y  A  +L+  +++  ++ FTTDT TS+  D+Y+ I AHFI  D  F  N + L + S PE HT VNL  +  E
Sbjct:   41 LPDMYNNANVELQDKMNKVEYMSFTTDTWTSVSMDSYMGITAHFI--DGDFNLNSYLLSMVSLPEQHTAVNLHTEFEE 116
[Close]
BLAST of SMED30003519 vs. Planmine SMEST
Match: SMESG000050347.1 (SMESG000050347.1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.828e-9
Identity = 36/95 (37.89%), Postives = 60/95 (63.16%), Query Frame = -3 
Query:  820 ILKRFIEQKQAIYATLSTQNKINLISKLNDDEYNVIDLIIKLLTLF*EATRYISRDKYATGSSILR**KQLKLCLI-REYQNNELKLVEEFYSDM 1101
            +++RF+EQ  AIYATL  QNK+NLI+ L +++Y ++  ++ +L  F EAT + S +K +T S IL    QLK+  I  + +     +++ FY D+
Sbjct:    1 MVERFLEQNNAIYATLLEQNKMNLINDLKNEDYIMLKELMMILKPFEEATTFGSAEKSSTASVILPTLNQLKIIAIDAKLKCEHTNILKNFYDDL 95
[Close]
BLAST of SMED30003519 vs. Planmine SMEST
Match: SMESG000007132.1 (SMESG000007132.1)

HSP 1 Score: 47.7506 bits (112), Expect = 2.160e-6
Identity = 29/90 (32.22%), Postives = 46/90 (51.11%), Query Frame = -1 
Query: 1170 INEIINNWNL-QNK*VAIEHDNAANISKATDILSFERIQCFAHSLQLAINHCLKRKEIQKIVGSFIGVV----GHFKHSTTASNMLEEQQ 1424
            + EI+  WN+  ++   I  DN A++  A  +  F+ + C+ H++QL +N  LK    Q  V   I V     GH +HST A+  L + Q
Sbjct:   14 LQEILREWNINDDRKGIIVSDNGASMKAAARLGGFQYLGCYIHTIQLVVNDELK-SSTQSSVADTIAVARSIAGHLRHSTQATEALRKIQ 102
The following BLAST results are available for this feature:
BLAST of SMED30003519 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ZBED44.961e-837.14zinc finger BED-type containing 4 [Source:HGNC Sym... [more]
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BLAST of SMED30003519 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C10A4.16.341e-628.00pep chromosome:WBcel235:X:7415839:7418627:1 gene:W... [more]
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BLAST of SMED30003519 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003519 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003519 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ZBED44.431e-937.14zinc finger, BED-type containing 4 [Source:NCBI ge... [more]
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BLAST of SMED30003519 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Zbed44.570e-735.71zinc finger, BED type containing 4 [Source:MGI Sym... [more]
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BLAST of SMED30003519 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|O75132|ZBED4_HUMAN2.181e-737.14Zinc finger BED domain-containing protein 4 OS=Hom... [more]
sp|Q80WQ9|ZBED4_MOUSE2.775e-635.71Zinc finger BED domain-containing protein 4 OS=Mus... [more]
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BLAST of SMED30003519 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
W4YN883.354e-2731.61BED-type domain-containing protein OS=Strongylocen... [more]
H3A9278.246e-2534.57Dimer_Tnp_hAT domain-containing protein OS=Latimer... [more]
A0A444UVN66.037e-2435.53Zinc finger BED domain-containing protein 1 OS=Aci... [more]
A0A3N0Y7T92.749e-2330.86Zinc finger BED domain-containing protein 1 OS=Ana... [more]
A0A1X7VJT83.308e-2334.55DUF659 domain-containing protein OS=Amphimedon que... [more]
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BLAST of SMED30003519 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000030725.11.446e-2534.55pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000057362.19.172e-2330.91pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000045953.11.095e-2231.74zinc finger BED domain-containing protein 1-like [... [more]
ENSAMXT00000046756.11.694e-1932.32pep primary_assembly:Astyanax_mexicanus-2.0:22:973... [more]
ENSAMXT00000047448.11.900e-1931.61pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of SMED30003519 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000001841.16.097e-1426.54pep scaffold:Pmarinus_7.0:GL477152:41279:42850:-1 ... [more]
ENSPMAT00000008230.16.447e-1030.59pep scaffold:Pmarinus_7.0:GL479508:145707:147445:-... [more]
ENSPMAT00000008876.12.033e-928.57pep scaffold:Pmarinus_7.0:GL476791:39849:44790:1 g... [more]
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BLAST of SMED30003519 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003519 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003519 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000035819.16.178e-2132.30pep primary_assembly:ASM223467v1:2:6014183:6016710... [more]
ENSORLT00000046033.17.143e-924.86pep primary_assembly:ASM223467v1:18:700339:706184:... [more]
ENSORLT00000037653.19.843e-726.49pep primary_assembly:ASM223467v1:21:546436:551984:... [more]
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BLAST of SMED30003519 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000034450.12.980e-1425.15SMESG000034450.1[more]
SMESG000045018.12.166e-1027.54SMESG000045018.1[more]
SMESG000026828.18.833e-1041.03SMESG000026828.1[more]
SMESG000050347.14.828e-937.89SMESG000050347.1[more]
SMESG000007132.12.160e-632.22SMESG000007132.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003519 ID=SMED30003519|Name=SMED30003519|organism=Schmidtea mediterranea sexual|type=transcript|length=2018bp
ATTAGAATGAGATTAATACCTAATATAAGGTTGAATCAAATTGTTTTAAG
TATGATAAAATGTAAAATCATTATGTTTGAAATTAGAAAAATCAATAATT
AAAAAATGCGACGAACTTAATATTTATTCAAATATTTGTAATTATACTTC
TAACATTTATACGTTTTTTGAAGTTAACCTATTACGTAAATCATCAACCG
CTATTCATGCACTTGAAAAAACACGTTCAGGTGACGCACTAGACCCAGGA
ATTAATAAGTATTTCATTGCCAGCTTATATAGTATAGGGAATTTTTTTAT
TCTCCTTCCAAAATTTAATTGGATTATTAGTTATTTTGCTGACTGGCATT
CTTTCATACTCTTCTATTTCCGACAATTCTAACATTTTTATTATAATTTT
TCCATAATGATGATTTAGTTGATGGAGTGTCAATAATAGCAGTGTTTTTT
TAAATTATCCATGAAATTAAATTCAGCTTTCACATATTCCTTAGTAATAT
ACGGAAAATTGCCATCTAAAAATTATCAAGGTAGGTTGCAATGCATATAG
TTTTTATAGAATAAACATAATTTTTTCTAATGTATCAATTTAATTTAACA
TATCACTATAAAATATCACTATAACATATCACTATTTAGTTGATGGAGTG
TCAATAATAGCAGTGTTTTTTAAATTATCCATGAACATTAAATTCAGCTT
TCACATATTCCTTAGTAATATACCGAAAATTGCCATCTAAAAATTATCAA
GGTAGGTTGCAATGCATATAGTTTTCATAGAATAAACATAATTTTTTCTA
ATGTATCAATTTAATTTAACATATCACTATAAAACTCTTCAACTAACTTT
AATTCATTATTTTGGTATTCTCGTATTAAACATAATTTTAATTGTTTTTA
TCATCGCAATATGCTTGATCCAGTAGCGTATTTATCCCGTGAAATGTATC
TGGTGGCTTCCTAAAATAGCGTTAAAAGTTTTATAATCAAATCTATCACA
TTATATTCATCATCATTCAATTTACTTATCAAATTAATTTTATTTTGTGT
CGATAGTGTAGCATAAATTGCTTGTTTTTGTTCTATAAAACGTTTCAATA
TGTACAAGACACTGTTTCATCTGGTTGGAACGTCCTGTATTAATGTATGA
ACAGAACATTTAATAATTTCTGTTGCTCTTCCAACATATTACTTGCTGTT
GTGCTATGTTTAAAATGTCCGACCACCCCTATAAACGATCCTACTATTTT
TTGTATCTCCTTTCTTTTCAAGCAGTGATTAATCGCTAACTGCAACGAGT
GTGCAAAACATTGAATTCTTTCAAAAGATAAAATATCTGTTGCCTTGGAT
ATATTTGCAGCGTTATCATGTTCGATGGCTACTCATTTATTTTGCAAATT
CCAGTTATTTATAATTTCATTAATTTTCAACATAAGATTTACATGTGTGT
GCATTTCGGGAAAACTTGAAATATCAAGGAAACAGTTATAGACGAAGGTA
CTATCTGGCATAATAAAATGCGCATTGATTGACAAATATGCATCTGTTAA
AATAGATGTTCAAGTATCGGTGGTGAATCTAACAAATATTGCTCTACTTA
AAAATGTTTCGAGTTGAGTTTTTGCTTGTTGATAGATGAGTGGCAGTCGT
AGAAGAAAATGTCCATCTATTTGGAATTTTAAAGATTGGATTTAATGGTT
CAGTATATGCAATACATTTTCCCCCTAGAAATGAAATAGGTAAACGGTTT
TCACATATAAGTGCATAAAGATAATATTCTACTTTTTAAAAATTTATTTC
TTTGTTGTTTTCATTCATTTCCTTATTTGTGTGAAATAATCTCACGTGTT
GTAACATGTTGTTTGTTGCGCCATTTGTTGAAACATTTTTGTTACATAAC
TTACAGACAGCGGATTTAAAATAGATTTCATTGAAGATGTCCCACTATGC
TGGCTCTTTTACATTTTTTGCTGATGGCTGACAAAAAGCTAATCTAACTT
GCTGAATACCGATTTCAT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000016pharynx
PLANA:0000099neuron
PLANA:0000484secretory system
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR003656Znf_BED
IPR011528NERD
IPR008906HATC_C_dom
IPR012337RNaseH-like_sf
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0046983protein dimerization activity
GO:0003676nucleic acid binding